LOCUS       DATDBZ010000001         2192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2864335, whole genome shotgun sequence.
ACCESSION   DATDBZ010000001 DATDBZ010000000
VERSION     DATDBZ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2864335"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VIX62_00005"
     CDS             complement(<1..263)
                     /locus_tag="VIX62_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEY5858650.1"
                     /translation="MTPAERRVARALLETYPTAGLESLPQLADGAGVTGPTVLRFVRK
                     IGFDGYPDFQRSLRQEVQARTEGLPSLSETRGSTPSDEVLRPP"
     gene            441..1649
                     /locus_tag="VIX62_00010"
     CDS             441..1649
                     /locus_tag="VIX62_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5858651.1"
                     /translation="MLKRSVALMFVLCLVAAACSTSDDTGDEGGDTGATGATGAPSGD
                     PIVIGFPADLTTDWAYYDSPMDEGAQFAVDQINDAGGVLGRPLELKTVDMRNDVAEAA
                     KVTQQLIDDGAVYLVGTVGDGILAEGQVACATGVPISTGLGTAPTLVGDMGECAYQLV
                     MSDNIQAAVLAEYALDEGYENAYVLGSNEIPYTRDLPIFFSDAFENGGGTVVGTDQYK
                     LGAGDYSAVVTKIDQADPEPDVIFTPMYIPDSVVFLRQLRQAGITTPVLSTDGNADAA
                     LLDAGAKAIDGLTFSNSVCTAEGDPNVQAFYDDYNAANGEDPSSYVAVIGYDEVNIVA
                     QAIEAAGSTDPAAIQEELAALDYTGVSGHVIMDPATRRANKPAALIQMDGEEFTCLGQ
                     PDFPDYVPSI"
     gene            1652..>2192
                     /locus_tag="VIX62_00015"
     CDS             1652..>2192
                     /locus_tag="VIX62_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5858652.1"
                     /translation="MTDDRGEDRVADATGSGPPLLVVDGLTVAYGAVVAIRDVSLTVG
                     RGEIVAALGPNGAGKTTLLRTIAGALKPQHGSVGFDGSPLTGLVPEVVVRRGVALVPE
                     GRHVFPNLTVQENLTIGGIARTDRDELRADTDRWLARFPILGERASQMAGTLSGGEQQ
                     QLAIARALMSRPRMLLLDEP"
BASE COUNT          372 a          715 c          760 g          345 t
ORIGIN      
        1 tggggcgggc ggaggacctc gtcgctcggc gtgctgccgc gggtctcgga gagggacggc
       61 agtccctcgg tgcgggcctg cacctcctgg cggagcgacc gctggaaatc ggggtagcca
      121 tcgaagccga tcttgcggac gaagcgaagg acggtcgggc ccgtgacccc ggcgccgtcg
      181 gcgagctggg gcaggctctc gaggccagcc gtgggatagg tctccaggag cgcgcgagcc
      241 accctgcgct cggcaggcgt catctcggct agtcgctcac ggacctcttc accgatcgtc
      301 tcgctcgtgt gcctgtcccc cttcccgaca cggacaggat tcttacctat ccgtgcagcg
      361 agcgaaagtc cgtaacggtc ttgacaggtc gaagggggca gcgtacgaat gccggatacg
      421 gggatcgggg aggttcaggg atgctcaaga gatcggtcgc actcatgttc gtgctgtgtc
      481 tggtggccgc agcgtgcagc acatcggacg ataccggcga tgagggggga gacaccgggg
      541 ctaccggtgc cacgggtgcc ccgtccggtg acccgatcgt catcggcttc ccggccgacc
      601 tcacgacgga ctgggcctac tacgactcgc cgatggatga gggcgcgcag ttcgcggtcg
      661 accagatcaa cgacgccggc ggcgtgttgg gccggcccct cgagctgaag accgtcgata
      721 tgcgcaacga cgtcgccgag gccgcgaagg tcacgcagca gctgatcgat gacggtgccg
      781 tctacctcgt gggcaccgtg ggcgatggca tcctcgccga aggacaggtg gcgtgcgcca
      841 ccggcgtgcc gatctccacg ggactgggca ccgcgccgac gctggtcggg gatatgggtg
      901 agtgcgcgta ccagctcgtc atgagcgaca acatccaggc agccgtgctg gccgaatacg
      961 ccctcgacga gggctacgag aacgcatacg tcctgggatc caacgagatc ccctacaccc
     1021 gtgatctccc gatcttcttc tccgacgcgt tcgagaacgg tggcggcacg gtggtgggca
     1081 ccgaccagta caagctcggc gcgggtgact actccgccgt cgtcacgaag atcgatcagg
     1141 ccgaccctga accggacgtg atcttcacgc ccatgtacat ccccgacagc gtggtcttcc
     1201 tgcggcagct gcggcaggcg gggatcacga cgccggtgct atcgaccgac ggcaacgccg
     1261 atgccgcgct gctggatgcg ggcgccaagg cgatcgatgg tctgacgttc tccaactcgg
     1321 tgtgcacggc cgaaggtgac cccaacgtcc aggccttcta cgacgactac aacgccgcca
     1381 acggtgagga tccgagctcc tacgtcgccg tgatcggcta cgacgaggtc aacatcgtcg
     1441 cccaggcgat cgaggcagcg ggcagcaccg accccgcggc gatccaagaa gagctggcgg
     1501 ccctcgacta cacgggagtc tccggccacg tcatcatgga tcctgcgacc aggcgggcga
     1561 acaagcctgc ggcactgatc cagatggatg gcgaggagtt cacctgcctg gggcagccgg
     1621 acttcccgga ctacgtgcct tcgatctgag cgtgaccgac gaccgagggg aggaccgggt
     1681 ggctgatgcc accggaagcg gtcctcccct gctcgtcgtc gacggattga cggtcgccta
     1741 cggtgccgtg gtcgcgatcc gagatgtctc gctgacggtg ggccgtggcg agatcgtcgc
     1801 agcgctcggc cccaatggag ccggcaagac gacgctgctg cgcacgatcg ccggagccct
     1861 gaaaccgcag catgggtcgg tgggcttcga cgggagcccg ctcacgggcc tcgtccccga
     1921 agtggtggtc cgtcgaggcg tcgcgctcgt gcccgaaggt cgccacgtgt tccccaacct
     1981 caccgtgcag gagaacctga cgatcggcgg gatcgcacgc accgatcgcg acgagctgcg
     2041 cgccgacacc gatcgttggt tggcgcggtt cccgatcctg ggggaacggg cgagccagat
     2101 ggcgggcacg ttgtctggcg gcgagcaaca gcagctcgcg atcgcgcggg ccttgatgtc
     2161 acgtccgcgg atgctgctcc tcgacgaacc ct
//
LOCUS       DATDBZ010000002         3978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1227579, whole genome shotgun sequence.
ACCESSION   DATDBZ010000002 DATDBZ010000000
VERSION     DATDBZ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227579"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1225)
                     /locus_tag="VIX62_00020"
     CDS             complement(113..1225)
                     /locus_tag="VIX62_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858653.1"
                     /translation="MDGDEARIEELERGFRRDGLPNLVVDYSAAEDVFTRALPFFALV
                     FAVEVVVAVDAELGWTGIALVAAVLATLVMAFGVMNAVRGQRFFSVPERVDTPELVAF
                     VVLPVLVSFAFGDRRLDALAVLAINLVIVGGTYVVVGFGVLSIVRWAGVRFFTQLRTT
                     LPVLVRAVPLLLFFSLVMFFTTEIWQVFSLSPTALFWTAIALFMVLGIVFLSVRLPGV
                     IREVETDADVGEDPLRRRERRNLEAVALISEGLQVLFVSAAVWLFYVVIGSLLVTEEI
                     RELWFTTSGSPGWTLSLLGEQVQIGPPLLRVATGVASFAGLYYAVAILVDSAYRDQFV
                     DTLTSQLRETFERRVEYLRLLHHREGITPADPSAGT"
     gene            1284..2012
                     /locus_tag="VIX62_00025"
     CDS             1284..2012
                     /locus_tag="VIX62_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY5858654.1"
                     /translation="MAGAPNEERTFASAVVLFAHPDDAEFMCGGTVAKWARDGCEVHY
                     IVCTDGSAGSNEPGARREDVAPIREREQRAAADVLGVKSIAFLGEPDGLLEVNPRTRK
                     MVTREIRRLRPEVIVAPDPSRLWFASGYINHWDHKQAGLLALTAVMPDAPTRVMFQEL
                     EEEGLEPYEIPNLYLSGNEPDAFVDITETIDTKIEALAQHVSEEGEAAAPWVRERARE
                     VGEPVGMEYAESFKAFRFVDDDEG"
     gene            2052..2549
                     /locus_tag="VIX62_00030"
     CDS             2052..2549
                     /locus_tag="VIX62_00030"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HEY5858655.1"
                     /translation="MDHDEPIELTAAERRDIEADLNDLGSMRAVFSPQGVKGVVIACQ
                     DCGANHFYEWELLRDNLEHMLSSGEPRMHEPAFDLVEEEYIQWDYGKGYIDALADTGL
                     QPDRTVEVTQCPWCQTPFGDEFAFCPVCGRTLATVRLYRELLDRGFDERDARAMLIRA
                     GYEAF"
     gene            complement(2566..>3978)
                     /gene="ligD"
                     /locus_tag="VIX62_00035"
     CDS             complement(2566..>3978)
                     /gene="ligD"
                     /locus_tag="VIX62_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY5858656.1"
                     /translation="GPEGPTEPAPGDVAAATGNSFVIHKHRATRLHYDVRLEKDGALP
                     SWAVPKGLPTVKGDKRLAVRTEDHPLEYGSFEGTIPEGHYGAGEVRIFDDGWYEPVEW
                     TDSKVSFILHGRRYPGLEFHFVKTRTDWLAFLASAQSAPLIPVPDRFQPMLAEGGWEA
                     FDDDGWWFEPKLDGIRCLADLATGETILRTRRGRDVTAQYPELHMMHELVDQVNAVVD
                     GEIVAFDERGRNSFEALQQRMNLQGQRAIARASKQIPVALVVFDLLWLDGHETTGLGL
                     EQRRELLELVVEEDPRLQVMSHVQGDGKSFTESARALELEGVVAKRMGSTYLPGRRSS
                     DWRKIKLTDTQDCVILGWTPGQGGRGTSFGALLVGAIDTDGEMRWIGQVGTGFTDTTL
                     KAVLAQLEPLVRADPPIDDPELAAFRGATFVEPELVCEVGYLEITKSTKKMRAPTFKG
                     MRPDKTPDECVLELRRGSRG"
BASE COUNT          680 a         1392 c         1311 g          595 t
ORIGIN      
        1 tcctcgccgc cggtggaact gcagccggtg atcaccctgc ggccgcgcta cggcatgccc
       61 atgcgcgggc accgccggga gcggccgtga cggttcgacg gtggcaacga cgtcacgttc
      121 ctgcggacgg gtcggcgggc gtgatccctt cgcggtggtg gagcaggcgc aggtactcga
      181 cgcgacgttc gaacgtctct cggagctggc tcgtgagggt gtcgacgaac tgatcgcggt
      241 aggcggagtc gacgaggatc gccaccgcgt agtagagccc ggcgaaggag gccaccccgg
      301 ttgcgacgcg cagcagcggc ggaccgatct gtacctgctc gccgagcagc gacagtgtcc
      361 agcctgggct ccccgacgtc gtgaaccaca gttcgcggat ctcttcggtc accagcagcg
      421 agccgatcac gacgtagaac agccacacgg ccgcactgac gaacaacacc tggagccctt
      481 cgctgatcaa cgccaccgcc tcgaggttcc gccgctcgcg tcggcgtaag gggtcctcac
      541 ccacgtccgc atcggtctcg acctcgcgga tcacaccggg gagccggacc gagaggaaca
      601 cgatccccag caccatgaac agggcgatcg cggtccagaa caacgccgtc ggcgagagac
      661 tgaagacctg ccagatctcg gtggtgaaga acatgaccag ggagaagaac agcagcaacg
      721 gcaccgcgcg caccaggacg gggagcgtgg tgcgcagctg cgtgaagaac cgcacgccgg
      781 cccagcgaac gatcgacagc accccgaacc cgaccaccac gtaggtgccg cccacgatca
      841 ccaggttgat cgccagcacg gcgagggcat ccagtcgccg gtcgccgaac gcgaaggaca
      901 ccagcacggg cagcaccacg aacgccacga gctccggcgt gtcgacgcgc tccgggacgg
      961 agaagaaccg ctgcccccgg acggcgttca tgaccccgaa ggccatgacc agggtggcca
     1021 acaccgcggc caccaaggcg atcccggtcc acccgagctc ggcgtcgacg gccaccacca
     1081 cctcgaccgc gaacaccagc gcgaagaagg gcagcgcccg cgtgaacacg tcctcggcgg
     1141 ccgagtagtc caccaccagg ttggggagtc catctcgccg gaacccgcgt tccagctcct
     1201 cgatccgcgc ctcgtctccg tccatcgggg catcgaacca catcgacgtg tgcggaagcg
     1261 tgaacagccg gtaggctcgt tgcgtggcag gagcaccgaa cgaagaacgg acgttcgcga
     1321 gcgccgtcgt gctgttcgcc catcctgacg atgcggagtt catgtgcggg ggcacggtgg
     1381 ccaaatgggc tcgcgacgga tgcgaggtgc actacatcgt gtgcaccgac ggatccgcgg
     1441 gttccaacga acccggagcg cgccgtgaag acgtcgcccc catccgcgag cgggagcagc
     1501 gcgccgcggc cgacgtgttg ggggtcaaga gcatcgcctt cctcggcgag cccgacgggc
     1561 tcctcgaggt gaacccgcgc acgcgcaaga tggtgacccg cgagatccgc cggctgcggc
     1621 ccgaggtgat cgtggcgccc gatccctcgc ggctctggtt cgcctccggc tacatcaacc
     1681 actgggacca caagcaggcg gggctgctgg cgctgacggc ggtcatgccc gatgcgccca
     1741 cgcgagtgat gttccaggaa ctcgaggagg agggcctcga gccctacgag atccccaacc
     1801 tctacctcag cgggaacgag cccgacgcct tcgtcgacat caccgagacg atcgacacga
     1861 agatcgaggc cctggcccag cacgtgtccg aagaaggcga ggccgccgcc ccttgggtgc
     1921 gcgaacgagc tcgcgaggtg ggggaaccgg tgggcatgga gtacgccgaa tccttcaagg
     1981 ccttccggtt cgttgacgac gatgaagggt gatgcccgcg cgagactcgc gcgaggccac
     2041 ggaccactcc catggatcac gacgaaccca tcgagctgac cgccgccgaa cgacgtgaca
     2101 tcgaggccga tctcaacgac ctcgggtcga tgcgtgccgt cttctcaccg caaggcgtga
     2161 agggcgtcgt catcgcctgc caggactgtg gggccaacca cttctacgaa tgggagctcc
     2221 tgcgcgacaa cctcgagcac atgttgagct cgggcgagcc ccggatgcac gaacccgcct
     2281 tcgacctcgt cgaggaggag tacatccagt gggactacgg caagggctac atcgacgccc
     2341 tcgccgacac cggtctccag cccgatcgca cggtcgaagt gacgcagtgt ccgtggtgtc
     2401 agaccccgtt cggggacgag ttcgcgttct gccccgtgtg cggtcggacg ctcgccacgg
     2461 ttcgcctcta ccgcgaactg ctcgaccgcg ggttcgacga gcgtgacgcg cgcgcgatgt
     2521 tgatccgcgc cggctacgaa gcgttctgac gcgtcgttcg gaccgtcacc ctcgtgagcc
     2581 cctgcgcagc tcgagcacgc attcatccgg cgtcttgtcc ggccgcatgc ctttgaaggt
     2641 cggggcgcgc atcttcttcg tgctcttcgt gatctcgagg tagccgacct cacacacgag
     2701 ctcgggctcc acgaacgtcg cccccctgaa ggcggcgagc tccggatcgt cgatcggcgg
     2761 gtcggctcgg acgagcggct ccagctgggc cagcaccgcc ttcagcgtgg tgtcggtgaa
     2821 gccggtgccc acctgcccga tccagcgcat ctcgccatcg gtatcgatcg cgcccacgag
     2881 cagcgcgccg aacgaggtgc cgcgccctcc ttggcccggg gtccacccca ggatcacgca
     2941 gtcctgggtg tcggtgagtt tgatcttgcg ccagtcggac gagcgacgac ccggcagata
     3001 ggtcgagccc atccgcttgg ccaccacccc ttcgagctcg agggcccgcg cgctctcggt
     3061 gaacgacttc ccgtcgccct gcacgtgcga catcacctgc aggcgcgggt cctcctccac
     3121 caccagctcc aggagctcgc gtcgctgctc caggccgagc ccggtggtct cgtgtccgtc
     3181 gagccacagc aggtcgaaca cgaccagggc caccgggatc tgtttcgagg cccgggcgat
     3241 cgctcgttgg ccctgcaggt tcatccgctg ctgcaacgcc tcgaacgaat tcctgcctcg
     3301 ctcgtcgaag gccacgatct cgccgtcgac cacggcgttc acctggtcga ccagttcgtg
     3361 catcatgtgc agctcggggt actgggccgt gacgtcgcgg ccgcggcggg tccgcaggat
     3421 ggtctcgccc gtggcgaggt cggcgaggca ccggatcccg tcgagcttgg gttcgaacca
     3481 ccatccgtcg tcgtcgaacg cctcccaccc gccctcggcc agcatcggct ggaaccggtc
     3541 gggcaccggg atcagcggcg ccgattgggc gctcgccagg aacgcgagcc aatccgtgcg
     3601 ggtcttcacg aagtggaact ccaggcctgg gtagcggcgg ccgtgcagga tgaagctcac
     3661 cttcgagtcg gtccattcca caggctcgta ccagccgtcg tcgaagatgc ggacctcgcc
     3721 cgcgccgtag tggccctcgg ggatcgtgcc ctcgaacgag ccgtattcca gcgggtgatc
     3781 ttcggtgcgc accgcgaggc gcttgtcgcc cttgaccgtg ggcaggccct tgggcaccgc
     3841 ccaactcggg agcgcgccgt ccttctccag gcgcacgtcg tagtgcagtc gtgtcgcccg
     3901 atgtttgtgg atcacgaacg agttcccggt cgcggcggcg acgtcgcccg gcgcgggttc
     3961 ggtggggccc tcgggtcc
//
LOCUS       DATDBZ010000003         3533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1227641, whole genome shotgun sequence.
ACCESSION   DATDBZ010000003 DATDBZ010000000
VERSION     DATDBZ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227641"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VIX62_00040"
     CDS             complement(<1..533)
                     /locus_tag="VIX62_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5858657.1"
                     /translation="MTQGRARRTLDADERRERIVETAMVHFAEHGYRGGRVEDIASQV
                     GIAKGTVFLDFGSKEGLFMAVYQRAVSKLPAWLDAPQDVVAQGFWATLDWWLERTEDF
                     VESDPVPHRIAVIGRYDTGMSMRRSIDRFMHSEDPYGTLEFVEFGVGQGEIRDDVDPE
                     MLASMLDWAAERFQDALV"
     gene            complement(1151..2416)
                     /gene="mshA"
                     /locus_tag="VIX62_00045"
     CDS             complement(1151..2416)
                     /gene="mshA"
                     /locus_tag="VIX62_00045"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HEY5858658.1"
                     /translation="MGHNPRVAVLSVHTSPMDQPGTGDSGGMNVYIRAVTERLAGRGI
                     DVDVFTRCRGGDDHEVKPLGEGIRVVSIKAGPCEPIPKSDIPRFLPEFLGGLLRIAHE
                     DAQGYDVVHSHYWLSGWVGRAAKHALDAPLVSSFHTLGKVKNYSLARGEPAEPAVRIA
                     GEQAVIDESDRVLAPTPSEASQLVGLYRADADQIRIVPPGVDHELFVPRDRAQARAAL
                     DLAGRRIALYVGRLQAHKGPDVAVRTVAEAIARDPDATEDLVLVIVGGPSGAEKGAEL
                     SRLLELAGALGVRDRLVLVPPQPQAELADYYAAADVVLVPSRSESFGLVALEAQACGT
                     PVVAARVGGLRYVVEDGRTGFLVEGHDPGDHAVRVLEVLTDAELQRGLGARGAERALR
                     FTWDATADAVLGVYREVLPARVFSPLHPA"
     gene            complement(2426..3403)
                     /locus_tag="VIX62_00050"
     CDS             complement(2426..3403)
                     /locus_tag="VIX62_00050"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY5858659.1"
                     /translation="MGAVPLARVVRSGLEESVHTGHVAVCDADGRLIARAGDPSHQVF
                     ARSCMKPLQASVSLASMDDLALPAREVAVMCASHNGEPVHLGAVRALLERASLGPEAL
                     QTPPDYPLDRDEMARAQHPNRVFHNCSGKHAGMLLACARAGWNVETYRRRSNPLQRRV
                     RSAVERATDIEDLVIGIDGCGVPVHGMPLQAMATLFARLARPERLGALAPAAGRATAA
                     MLAEPYLVGGRHRLDTEVIRVTGDVVVKEGAEALICASIQPQGVGIAVKVADGGYRAY
                     APALIEVLRQVDAVSGPQLRELATFARPPVIGGGERVGEVEPLVTLRRR"
BASE COUNT          546 a         1314 c         1177 g          496 t
ORIGIN      
        1 accagcgcgt cctggaaccg ttcggcggcc cagtccagca tggaggcgag catctccggg
       61 tcgacgtcgt cgcggatctc cccctgcccc acgccgaact cgacgaactc caacgtgccg
      121 tatggatctt cgctgtgcat gaaccggtcg atcgagcgcc gcatgctcat gccggtgtcg
      181 tagcgcccga tgacggcgat gcgatggggc acggggtcgg attcgacgaa gtcctcggtg
      241 cgctccagcc accagtcgag cgtggcccag aacccctggg ccaccacgtc ctgcggggca
      301 tcgagccagg ccgggagctt cgagaccgcc cgctggtaca cggccatgaa caggccttcc
      361 ttcgaaccga agtccaggaa gacggtcccc ttggcgatcc ccacctgcga ggcgatgtcc
      421 tcgacgcgcc cgccgcggta cccgtgttcg gcgaagtgga ccatggcggt ctcgacgatg
      481 cgttcacggc gttcgtccgc gtccagcgtc cggcgggctc gaccctgggt catgacggaa
      541 cgctaacgga aaaccgccgc cgacccggga ccggacaaga aagaacccga gagacctacc
      601 ggctcgccgg gaaccgaagc tcccagccac cgtcggcctc tcgggctctg gtgccccaag
      661 caacctccgc gccgtcacag cggggaccga cgccctggcg gcggctcgcc cgaaggcgct
      721 ccagccgcct cgccacctgg tggtcggcct ttcggccttc ctccgcggca gcgggctccc
      781 cgcccaggtc caccttgcgg cgttccccga cgggcacatc gcgtcggcct tgcggctctc
      841 gcgacgccgg atcgtcgtcc gggtcttgcg acccttcttc cgatccggtg gtccgcttcc
      901 aggtcttgcg acccttccgc tcgccctccg ccccttcccc gtcttgcgac ggttccggcg
      961 cgggtccggt ctcctcgagt cggcacttcg actgcttgga gcgaggcgaa gactacgagc
     1021 ggcccgaggc agggtcaact ccacatctcc gcaggtcagg gcatatatct gcaggtcaga
     1081 ggctcaggac aaagagtgac aattcgcgcg cgaccgtgac gacgatcacg gcgaggggag
     1141 aacgtgcagg tcatgcgggg tgcagcggcg agaacacccg ggccggcagc acctcacggt
     1201 agacacccag gaccgcatcg gcggtcgcat cccaggtgaa ccggagggcc cgctcggccc
     1261 ctcgggcccc gagcccacgc tggagctcgg cgtcggtcag cacctccagc acacgcaccg
     1321 cgtggtcgcc ggggtcgtgg ccttcgacca ggaacccggt ccggccgtcc tcgaccacgt
     1381 agcgcaagcc acccaccctg gcggccacca ccggcgtgcc gcacgcctgc gcctccaacg
     1441 ccacgagccc gaacgactcc gacctcgacg gcacgagcac cacatcggcg gcggcgtagt
     1501 agtcggccag ctcggcttgc ggctgcggcg gcaccaggac caggcggtcg cgcaccccga
     1561 gggcaccggc cagttccagg agccgcgaga gctcggcgcc cttctccgcg ccgctcggac
     1621 cgccgacgat caccaacacc agatcctcgg tggcatcggg gtcgcgggcg atcgcctcgg
     1681 ccacggtgcg caccgcgacg tcagggccct tgtgtgcctg cagcctgccc acgtagaggg
     1741 cgatccgccg gccggccagg tcgagtgccg cccgtgcctg cgcgcgatcc cgcggcacga
     1801 acaactcgtg gtcgacgccc ggtggcacga tccggatctg atccgcatcg gcgcggtaca
     1861 gccccaccag ttgcgaggcc tccgagggcg tgggggccag gacgcgatcc gattcgtcga
     1921 tcaccgcctg ctcgcccgcg atccggaccg ccggctcggc cggctccccg cgagccaggg
     1981 agtagttctt caccttgccc aacgtgtgga acgaggacac cagcggcgcg tcgagcgcgt
     2041 gcttggccgc ccgacccacc cagccggaga gccaatagtg gctgtgcacg acgtcgtagc
     2101 cctgcgcatc ttcgtgcgcg atgcgcagca ggcccccgag gaactcgggg aggaaccggg
     2161 ggatgtcgga cttcgggatg ggctcgcacg gaccggcctt gatcgagacc acccggatgc
     2221 cctcaccgag gggcttcacc tcgtggtcgt cgccgccgcg acatcgggtg aacacgtcga
     2281 cgtcgatgcc gcggcccgcg agccgctcgg tgacggcgcg gatgtagacg ttcatcccgc
     2341 cggaatcacc ggtgcccggt tggtccatcg gggacgtgtg caccgagagc accgcgaccc
     2401 gcggattgtg gcccatcgag gaaggctacc gtcgccgcag cgtaacgagg ggttcgacct
     2461 cgcccacccg ctcccctcct ccgatcacgg gcggccgggc gaatgtcgcc agctcgcgga
     2521 gctgcgggcc gctcacggca tcgacctgtc ggaggacctc gatcagcgcc ggcgcgtagg
     2581 cgcgatagcc cccgtcggcg accttcacgg cgatccctac cccctgcggc tggatcgatg
     2641 cgcagatcaa cgcctcggcg ccctccttga ccacgacgtc gccggtgacg cggatcacct
     2701 cggtgtcgag ccgatgccgg ccaccgacca ggtagggctc ggcgagcatc gcggccgtcg
     2761 cccggccggc cgcgggtgcc agcgctccca ggcgctcggg tcgggcgagc cgcgcgaaca
     2821 gcgtcgccat cgcctgcagc ggcatcccgt gcaccggcac cccgcacccg tcgatgccga
     2881 tcacgaggtc ctcgatgtcg gtcgcccgtt ccaccgccga tcgcacacga cgctgcagcg
     2941 ggttggaccg acgccgatag gtctcgacgt tccaacccgc ccgtgcgcac gccagcagca
     3001 tgccggcgtg cttgcccgag cagttgtgga acacgcggtt gggatgttgc gcgcgagcca
     3061 tctcgtcacg gtcgagcggg tagtccggcg gggtctggag cgcttcgggc cccagtgatg
     3121 cccgttccag cagcgctcgg accgcaccga ggtggaccgg ctctccgttg tgcgaggcgc
     3181 acatcaccgc gacctcgcga gcgggcagcg ccaggtcgtc catcgacgcg agcgacaccg
     3241 acgcctgcag cggtttcatg cacgagcgcg cgaacacctg gtgggaggga tcgccggccc
     3301 gggcgatcag ccgaccgtcg gcatcacaga cggcgacgtg cccggtgtgg accgattcct
     3361 ccagaccgga ccgaaccaca cgcgccagcg gcacggcacc catggaggtg gagcgtagac
     3421 cgaactcaga cggcctgagc gatctccgct tcctcgtgcg cgggttcggc ccagaggcca
     3481 ccctcgctcg ccagatcgag gaagacctcc acgatgtcgg gatcgaactg ggt
//
LOCUS       DATDBZ010000004         2111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1432813, whole genome shotgun sequence.
ACCESSION   DATDBZ010000004 DATDBZ010000000
VERSION     DATDBZ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432813"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..768)
                     /gene="rpsG"
                     /locus_tag="VIX62_00055"
     CDS             complement(298..768)
                     /gene="rpsG"
                     /locus_tag="VIX62_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY5858660.1"
                     /translation="MPRKGPAPRRKLMPDPVYRSLLVTQLVNKILLSGKRSIAEKIVY
                     DALKIVEEKTGAEPVATLKRAMDNVKPPLEVKSRRVGGATYQVPIEVRAPRANTLAIR
                     WVVNYSRARREKTMAQRLANELMDAANGLGASIKRRDDVQKMAESNKAFAHYRW"
     gene            complement(774..1145)
                     /gene="rpsL"
                     /locus_tag="VIX62_00060"
     CDS             complement(774..1145)
                     /gene="rpsL"
                     /locus_tag="VIX62_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY5858661.1"
                     /translation="MPTIQQLVRHGRSTKSEKSKTPALKGSPQRRGVCTRVFTTTPKK
                     PNSALRKVARVRLTSGVEITAYIPGEGHNLQEHSIVLVRGGRVRDLPGVRYKIIRGAL
                     DAAGVKNRKQARSRYGAKKDK"
     gene            complement(1239..>2111)
                     /locus_tag="VIX62_00065"
     CDS             complement(1239..>2111)
                     /locus_tag="VIX62_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY5858662.1"
                     /translation="TMRTFHTGGVAGKDIAGGLPRVVELFEARTPRGAARLARASGVV
                     RIGEDEAKGIPVTIVTDEGTEETVILPLQSRLLVEDGQEIRAGEPITDGPYDPKELME
                     IKGPRETQVYLVEEVQRVYRDQGVSIHDKHIELIVRQMTRRIGVQEPGETGFLPGERA
                     DAKVFRDTNKRTVESGGKPAEGRPELMGITKASLATESWLSAASFQETTRVLTEAAID
                     GRGDHLIGLKENIIIGKLIPAGSGMTKYREFDVAAPDYVPMSYYSSEMDDPAAYLASL
                     QGSYNGDGEALAEG"
BASE COUNT          307 a          703 c          711 g          390 t
ORIGIN      
        1 agtagaggat ccgctccgtc gtcgtggtct tgcccgcgtc gatgtgcgcc atgatcccga
       61 tgtttcgggt gcggtcgagg ggatagtcac gctttgccat cgtgttcact tccggttcat
      121 tcgggcgggg gtgcggaagg ccgggcgcgt agcgctgcac cggccggcgg cacgaaaatc
      181 aatgctatcg ggcgcgcctc gccgttcacc caaaccggct gacctgggga tgtgtcaccc
      241 cagagcgcgg aacggcccgc cgggaaggcg ggccggtcac gaggcagtcg ggcgcagtta
      301 ccagcggtag tgggcgaacg ccttgttcga ctctgccatc ttctgcacgt cgtcacgacg
      361 cttgatcgag gcgcccagac cgttggccgc atccatcagc tcgttggcga gccgctgcgc
      421 catcgtcttc tcgcggcggg cccgggagta gttcacgacc caccggatcg ccagcgtgtt
      481 cgcacgcggg gcgcggacct cgatcggaac ctggtacgtg gcaccgccga cgcgacggct
      541 cttgacctcg agcggcggct tcacgttgtc catcgcccgc ttcagcgtgg cgaccggttc
      601 ggcgccggtc ttctcttcga cgatcttcaa cgcgtcgtac acgatcttct cggcgatcga
      661 gcgcttgccc gacaggagga tcttgttgac gagctgcgtc acgaggagcg accggtacac
      721 ggggtccggc atcaacttgc ggcggggggc ggggccttta cggggcattc agatcacttg
      781 tccttcttcg cgccgtaacg gctgcgggcc tgcttgcggt tcttcacacc ggccgcatcg
      841 agcgcgcccc ggatgatctt gtaacggaca cccggcagat cgcggacccg cccgccccgc
      901 accagcacga tcgagtgctc ctgcaggttg tggccctcgc cggggatgta ggccgtgatc
      961 tcgaccccac tcgtcagccg cacgcgggcg accttgcgca gggccgagtt cggcttcttc
     1021 ggcgtggtgg tgaacacgcg ggtgcacacg ccgcgacgct gcggcgatcc cttcagggct
     1081 ggcgtcttgg atttctcgct cttggtgctg cgaccatgac ggaccagctg ctgaatggtg
     1141 ggcacgcgtt acctctgagt agttctcgga accggacaag gttaggggtc gccctgttcg
     1201 cacggcaacc tcggtggtgg aagtggcaga gcaggcgctc agccttcggc aagggcctcg
     1261 ccatcgccgt tgtacgagcc ctgcaggctc gccaggtagg ccgcgggatc gtccatctcc
     1321 gacgagtagt acgacatcgg cacgtagtcg ggcgcggcga cgtcgaactc gcggtacttc
     1381 gtcatgcccg aaccggccgg gatgagcttg ccgatgatga tgttctcctt gagcccgatg
     1441 agatggtcgc cgcggccgtc gatggcggcc tcggtgagca cccgggtggt ctcctggaac
     1501 gatgcggccg agagccacga ctcggtggcg agcgatgcct tggtgatgcc catgagctcg
     1561 ggccgtccct cagcgggctt gccgccggac tcgaccgtgc gcttgttggt gtcgcgaaac
     1621 accttggcgt cggcccgctc accgggcagg aagccggtct cgccgggctc ctgcacgccg
     1681 atgcggcgcg tcatctggcg cacgatcaac tcgatgtgct tgtcgtggat cgacacaccc
     1741 tggtcgcgat agacccgctg gacctcttcg accaggtaca cctgcgtctc gcgcgggccc
     1801 ttgatctcca tgagctcctt cgggtcgtac gggccgtcgg tgatcggctc accggcacgg
     1861 atctcctggc cgtcctcgac cagcagccgg ctctgcaacg gcaggatcac cgtctcctcg
     1921 gtgccctcgt cggtgacgat cgtgacgggg atgcccttcg cctcgtcctc accgatgcga
     1981 accacaccgg acgcacgggc cagacgagcc gcgccccgag gggtacgggc ctcgaacagc
     2041 tcgacgacgc ggggcagacc gccggcgatg tccttcccgg caacaccgcc ggtgtggaag
     2101 gtccgcatgg t
//
LOCUS       DATDBZ010000005         9566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1228393, whole genome shotgun sequence.
ACCESSION   DATDBZ010000005 DATDBZ010000000
VERSION     DATDBZ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228393"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VIX62_00070"
     CDS             complement(<1..1308)
                     /locus_tag="VIX62_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY5858663.1"
                     /translation="MSDVPSGDAPTHGHGRVARIAVAVTATLSLLIGVGSAYGFVAYR
                     QAGAEGTNPSGEWIPPRADGEGSTSDDPCARDVCNYLLLGSDSRAGLTSEERDQFGTD
                     QEIGGENRADTIMLVHTDPNLEKAIILSFPRDLWVQIPGQGWDKINAAFEGGLDGGGP
                     LLMAKTVQKLTGLPIDHTLYVDLAGFQGLVETLGGVDMCISGENVNTPGSVSAPGGGT
                     VYYEEQGFIADPYTGLLVQPGCNTLPAEQALAYVRTRHLRCDAAAPDFFRIARQQQFL
                     RAVINELLKPDQLAQLPFQIKPILRNLERDDGLRIADLAYLVGQLEGISSGAAEFRTV
                     PGIPTTVDGLAVIRLDPAAKQMFTAIGDGKQLGSTGTAVYTQSEATIPVLVVDHASGG
                     AAAEVQEVLSQAGFDISPGTTTSTAYQEKVAGSVIAYAPDADAE"
     gene            1479..2279
                     /locus_tag="VIX62_00075"
     CDS             1479..2279
                     /locus_tag="VIX62_00075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5858664.1"
                     /translation="MDDAVTVVLVGLETAMTDELADDERFDVRAAESIDQIAAGERVD
                     AVVVAMDGQGPLELLGSLRGKAPEAAILVVTEAGREADGAVAMHAGAEDHLVVGSIPH
                     GLLPRAVQYAVSIRRLRRELATTDEATGLPNLRGFVPIAEHHLRMADRSQTPVVFLFV
                     RLEGETDDDDAREAAEVLLEAVRDADLPARITADTFAVLLTGDAAGAETLVLSRLVEA
                     IAVHNAKQDPPRPLSLSVGSALYEPASPNTLTEILETAGRRLRDHSEG"
     gene            2344..2961
                     /locus_tag="VIX62_00080"
     CDS             2344..2961
                     /locus_tag="VIX62_00080"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5858665.1"
                     /translation="MSQAMLMLERSAPIDMGDLPEAGDRDLMARLHRGDEEAFRGLFH
                     RYAPTANALAQRVVRQSHLAEEIVQEAFMAVWRDPGAYDGERGSVRSWLMGMVHHRAV
                     DLVRREEAHRRRAEAAIPQAIEEQADHADDVVQQLGLPEERRIVRAALADLPEEQRDV
                     LESMYFDGLSQSQIAEKTGIPLGTVKSRTMLGMRRMRDVLAEVER"
     gene            2958..3698
                     /locus_tag="VIX62_00085"
     CDS             2958..3698
                     /locus_tag="VIX62_00085"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY5858666.1"
                     /translation="MTRDHTVIEELMAVDALGGLDGDDRALLERERESHGADCAECAL
                     LEKGFAETAGRLAFALDAEPVDDAVVDRILGSSDGSPASEAVVEIPQAPRDEVEERRA
                     RRPRVWLVAAAAAAAVVAIAVVAAGTIVPSTTSVQATTSQRLVVFTGETEGTLAMAFT
                     PGEPGAVFWGSDLPDPGADRVYEIWMIEGDEAVSGGCVTPTDGVIAVRVEASIGTTDT
                     MAVTTEPSDCPSTPSSDPILTADLTTMV"
     gene            3831..4229
                     /locus_tag="VIX62_00090"
     CDS             3831..4229
                     /locus_tag="VIX62_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858667.1"
                     /translation="MNKRSALMLSAALVLTLIVGGLAVATGLTGPTVSNAVPRAERRA
                     SSEPVVRTVRRTVKVHKKADAKPGEVVTVAAPAAAAASPSSSNDLSGDDDSYEDDDAH
                     EDEGDDHGDGGGDHGDHEDEGAHEDESDDD"
     gene            4557..5474
                     /locus_tag="VIX62_00095"
     CDS             4557..5474
                     /locus_tag="VIX62_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5858668.1"
                     /translation="MEHAPTERLDTPAMGTTLSVYGPGDGFAAGAEAVVETFRVEEQR
                     FSRFRPDSELSMVNARAGTWVTLSPGFESLLRSCLDQAAATGGAFDPTVLGAVEAAGY
                     DRDLDEVIRSARVVLRRPVPCGRWTEIERTDGWVRLPAGVGLDLGGIAKGWTVDLAVE
                     RALASGMSWVLVSAGGDLRVGGDAPPLAIDVEDPVDPSSAIMTVQIDRGALATSSTAK
                     RSWGPGLHHVIDPRTGAPAITDAVQVTSWAPTCAQAEVAATVALLRGTRAVADAPSVI
                     VGRDGTIFHSVTSSRSGEPSDEPEDEVAA"
     gene            5471..6049
                     /locus_tag="VIX62_00100"
     CDS             5471..6049
                     /locus_tag="VIX62_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY5858669.1"
                     /translation="MNTWILLRAAGIGSYLMLFLSIAWGLVATTSVVSTRISKPSANL
                     FHAFAATTGLALLGVHLGLLLIDEFMPFSVADLTVPMASEYRPVAITAGVVAMYAVVV
                     IVVSSWLRKPLGTAWWRRLHLLAVPAFALALAHGVFAGTDTERPWMFAMYAVTGLLVV
                     FLTLVRAFTYGYRPARPAPPVRTRPTEPVTPA"
     gene            complement(6012..6692)
                     /locus_tag="VIX62_00105"
     CDS             complement(6012..6692)
                     /locus_tag="VIX62_00105"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5858670.1"
                     /translation="MSRTVADVMTRTVVVVPGSAPFKQVVRAMREYRVSALPVTDPDG
                     VIEGVVSEADMILREDPSVLEPHFLEGHTRRDERKKAEALVARDLMTAPPVTVGPQAS
                     VADVARLMHERRVKRLPVVDLEGRIVGIVSRMDLLAEFLRDDDDIKAEVCRVLTDDLT
                     VVADQILVLVDDGVVHLEGRVERRSLVLTIWGAVRAVPGVVGLDERLTWELDDTITPA
                     SPVPWVGF"
     gene            6832..7995
                     /locus_tag="VIX62_00110"
     CDS             6832..7995
                     /locus_tag="VIX62_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY5858671.1"
                     /translation="MSDREQAPSVPPDETRTRMLIEAGLALSSELDLDSVLERIVELA
                     LGITGARYGALGVLGEDAPRIERFITQGVTDEERAAIGHLPVGHGILGLLIEERRPMR
                     IPEIGADPRSAGFPPNHPPMHSFLGAPVKALGQIFGNIYLTEKQGAPEFTDDDEAALV
                     VLATQAGVAIENARLYDEMRRAHEELARLELLEERERIAKELHDGVIQALFAVGMGLQ
                     GTAAMANDPEIARRIENATEEVDRAIRDLRNYIFGLRPGILADRQLDQALQQLALEFQ
                     ERTDVLTIVDVDGSVAAELASRAADVVQLVREGLSNVGRHAQATSCRITLRRSNGGAE
                     LEIDDDGLGFDPSSPSSGMGMPNLRERVTSLGGELTILSVAGEGTTIRAQLPL"
     gene            complement(8057..9421)
                     /locus_tag="VIX62_00115"
     CDS             complement(8057..9421)
                     /locus_tag="VIX62_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY5858672.1"
                     /translation="MRRFRMGALALATVLALVAVACSGDGEQAPAAGDGEAAAAGGPV
                     TVDVMLSDFAIEPAAIEVPAGSDITFNVMNHGQSPHTFGVVVNGGTIETPSIDVDGST
                     TLEVPALAAGTYDTLCTVPGHDQLGMVGTLTASDGATEGTTAASGGGTDATSHAAMTA
                     EEMAAGHEEGVQDFLAGKETDSYGNQPMEPEMDGGVKVYNFTVEEIQWEIAKGEFVDA
                     MAFNGQVPGPEVRVNEGDRVRFVVQNQMTEPFVLHFHGLTVPNSEDGVPYVTQPPIMP
                     GEYWTYEFTIKDPPGMYVYHSHFNSAEQVDKGLYGSLIVEPKNANWTAVYGADPAVEY
                     TMFVGDGTLGYNLNGKSFPATQPIVASKGDWVLIHLANDGALLHPMHLHGFHFLVVGE
                     DGYPLAKQNRYLADTLVIAPGSRFDIMVKADYPGAWAFHCHILPHAEGPGGMFGMVTA
                     LVVQ"
BASE COUNT         1486 a         3306 c         3304 g         1470 t
ORIGIN      
        1 ctcggcgtcg gcgtcgggcg cataggcgat cacgcttccc gccaccttct cctggtaggc
       61 ggtggaggtg gtcgtgcccg gcgagatgtc gaacccggcc tgcgagagca cctcctgcac
      121 ctcggccgcc gcgcctcccg atgcatgatc gacgacgagg accggaatcg tcgcttcaga
      181 ctgcgtgtac acggccgttc ccgtcgatcc aagctgcttg ccgtcaccga tggccgtgaa
      241 catctgcttc gctgcgggat cgagacggat gacggcgaga ccatccaccg tggtcgggat
      301 gccgggcacc gttcggaact ccgccgcgcc cgacgatatg ccttcgagct gtccgacgag
      361 gtaggcaagg tccgcgatcc tgaggccatc atcacgctcg aggttgcgga ggatcggctt
      421 gatctggaac ggaagctgag cgagctgatc gggtttcagc agttcgttga tgaccgcacg
      481 caggaactgc tgttgtctcg cgatgcggaa gaagtcgggc gcggccgcat cgcacctcag
      541 gtgtcgggtt cgtacatatg cgagcgcctg ttcggccggc agcgtgttgc agccgggctg
      601 gaccaacaaa cccgtatacg ggtcggcgat gaagccctgc tcctcgtagt agacggtgcc
      661 acctcccggc gccgagaccg aacccggcgt gttcacgttc tcgcccgaga tgcacatgtc
      721 caccccgccg agggtctcga ccagtccttg gaagcccgcg agatccacgt acaaggtgtg
      781 gtcgatcggc aggcccgtga gcttctgaac ggtcttcgcc atcaagagcg ggccgccgcc
      841 gtccagaccg ccctcgaacg ccgcgttgat cttgtcccac ccttgaccgg ggatctgcac
      901 ccacagatcc cgggggaacg acaggatgat cgccttctcg agattcgggt cggtgtgaac
      961 aagcatgatc gtatcggcgc ggttctcccc cccgatctcc tgatcggttc cgaactgatc
     1021 gcgttcctcc gacgtgagcc cggccctgga gtcgctgccc agcaggaggt agttgcacac
     1081 gtcgcgcgcg cacggatcgt ccgaagtcga cccttcgccg tcggccctcg gggggatcca
     1141 ttccccgctt gggttcgttc cctcggcacc tgcctgtcga taggcgacga atccgtaggc
     1201 gctgccgacg ccgatcagca gggagagcgt ggccgtgacc gcgacggcga tgcgggcgac
     1261 gcggccgtga ccgtgggtgg gggcgtcgcc ggagggcacg tcggacatgc cgccaagggt
     1321 acccgtgcga tcagaagacc cgccccactg gcctcgtggt gatcgtgtgg cggaaccgtg
     1381 gcggcgcagg ggctcccgcc tcgccgggct gccccggccg gtgccctgcg gcgcgagggt
     1441 tcccggatgg gataatcccg ccatcgacag gaggccagat ggacgatgcg gtgacggtgg
     1501 tgctcgtggg gctcgagacg gcgatgaccg acgagctcgc cgacgacgag cggttcgacg
     1561 tgcgtgctgc cgagagcatc gatcagatcg cggcgggcga acgggtcgac gcagtggtcg
     1621 tcgcgatgga cggccaggga ccgctcgagt tgctcggctc cttgcggggc aaggcacctg
     1681 aggccgcgat cctggtcgtc accgaggcag ggcgcgaggc cgacggcgcc gtggcgatgc
     1741 atgccggcgc cgaggaccat ctcgtcgtcg gctcgatccc ccacggactc ctgccccggg
     1801 ccgtgcagta cgcggtgtcg atccgacggc tccgtcgcga gctcgccacg accgacgaag
     1861 cgaccggcct gccgaacctg cgcgggttcg tgccgatcgc ggagcaccac ctgcgcatgg
     1921 ccgatcgcag ccagaccccg gtggtgttcc tgttcgtgcg gctcgagggc gagaccgacg
     1981 acgacgacgc gcgcgaggcg gctgaggtgc tgctcgaggc cgtgcgcgac gccgatctgc
     2041 cggcgcggat caccgccgac acgttcgccg tgttgctgac cggtgacgcg gccggcgccg
     2101 agaccctcgt gctctcccgt ctcgtcgaag cgatcgccgt gcacaacgcc aagcaggatc
     2161 ctccccggcc actctcgctc tcggtcggca gcgccctgta cgaaccggcg tcgccgaaca
     2221 ccttgaccga gatcttggag acggcgggtc gcaggctgcg cgatcactcg gagggctgag
     2281 ccatccgatc aacctcgcgc tgcgaatacc ccgctgaacg atctcgctcc gaggaaggtg
     2341 ccgttgtccc aggcgatgct gatgctggaa cggagtgcgc cgatcgacat gggtgacctg
     2401 cccgaggcgg gcgatcgcga cctgatggct cgcctgcatc gcggcgacga ggaggcgttc
     2461 cgagggttgt tccatcggta tgccccgacg gccaatgcgc tcgcgcagcg ggtcgtgcgg
     2521 caatcgcatc tggcggagga gatcgtgcag gaggcattca tggcggtctg gagggatccg
     2581 ggcgcctacg atggcgagcg aggctcggtg aggagctggc tcatgggcat ggtgcatcat
     2641 cgtgccgtcg atctcgtgcg ccgagaagag gcgcatcgtc gccgcgccga ggcggccatc
     2701 ccccaggcga tcgaggagca ggcggatcac gccgacgacg tcgtccagca gctggggctg
     2761 cccgaggagc gcaggatcgt gcgcgccgcc ctcgccgacc tgcccgaaga acagcgggat
     2821 gtgctcgagt cgatgtactt cgacgggttg tcccagtcgc agatcgccga gaagaccggt
     2881 atcccgctcg gcaccgtgaa gtcccggacg atgctcggca tgcgccggat gcgcgacgtt
     2941 ctcgcggagg tcgaacgatg acgcgcgacc acaccgtgat cgaagagctc atggcggtcg
     3001 acgccctcgg cggactcgat ggcgacgacc gggccttgct ggagcgcgag cgcgagtcgc
     3061 acggagcgga ctgcgccgag tgcgccctgc tcgagaaggg gttcgccgag accgcgggcc
     3121 ggctggcgtt cgccctcgac gcggaaccgg tggacgacgc cgtcgtggat cgcatcctgg
     3181 gctcgagcga cggatcgccg gcgagcgagg ccgtcgtcga gatcccgcag gctccgcggg
     3241 acgaagtcga ggagcgccgg gctcggcgcc cgcgtgtctg gctcgtggcc gcggccgcgg
     3301 ctgcggccgt cgtcgcgatc gcggtcgtgg ccgccggcac gatcgtgccc tcgacgacga
     3361 gcgtgcaggc gacgacgtcg cagcgcctcg tggtcttcac gggcgagacc gagggtaccc
     3421 tcgcgatggc cttcacccca ggtgagccgg gagcggtgtt ctggggcagc gacctccctg
     3481 accccggcgc tgacagggtc tacgagatct ggatgatcga aggcgacgag gccgtgtcgg
     3541 ggggatgcgt gaccccgacg gacggggtga tcgcggtccg ggtcgaggcg agcatcggca
     3601 ccaccgacac gatggcggtg acgaccgagc cgtcggactg cccctcgaca ccctcgagcg
     3661 atcccatcct gaccgccgat ctgaccacga tggtctgatc cactcggccg ccccgccgga
     3721 aggcgaggct tacccgaacc ttaaacccgc cttgcccggg cccttgatga caccccccta
     3781 gcgtggtcct cggcgggaac gagcccgccg tgacccgaag gagatccggg atgaacaagc
     3841 gctccgcact gatgttgtca gcagcactcg tgctcaccct gatcgtcggt ggcctcgccg
     3901 tggccacagg actgacgggc ccgaccgtca gcaacgcagt cccgagggcg gaacgtcgcg
     3961 cctcatccga gccggtcgtc cgcaccgttc gccgaaccgt gaaggtgcac aagaaggcgg
     4021 acgcgaagcc gggcgaggtc gtgacagtcg cggctccggc agcggcggcc gcctccccga
     4081 gcagctcgaa cgacctgtcg ggagacgatg attcctacga ggacgacgac gctcacgagg
     4141 acgagggcga cgaccacggg gacggcggcg gggaccacgg ggaccacgag gacgagggcg
     4201 cccacgagga cgagagcgac gatgactgac gcacctcgcc ggaagcctcg ccggaagcct
     4261 cggtggagcc gccaccgact gcgcgcactc gcgtggacga ccgggatcgc cacattcctg
     4321 gcgggcgtcg gtgccctggg tgcctccccg atgcccgagc aggcgcagcc gccgggggac
     4381 cgctgggtcc cccggcaacg cgtgatcgtg cggcgcatca cccggcgcat cgtcgtggtg
     4441 gaacccgccg ccagcgcacc gatcacctac gtccaggccc cgtcggtgag tagttcgtcg
     4501 agctcatcgg gtggcgcacc tgcccctgca ccgccgcctt ccacgggagg atcgtgatgg
     4561 agcacgctcc caccgagcga ctcgacaccc cggcgatggg caccacgctc agcgtctacg
     4621 gaccgggcga cggattcgcc gccggagccg aggcagtggt cgagaccttc cgggtcgagg
     4681 agcagcggtt cagccggttc cggcccgaca gcgagctgtc gatggtaaat gcccgtgcgg
     4741 gcacatgggt gaccctctcc cctggattcg agtccctgct ccggtcctgc ctggatcagg
     4801 cggccgccac cggcggagcg ttcgatccga cggtgctcgg tgccgtcgag gccgccggat
     4861 acgaccgcga tctcgacgag gtcatcagat cggctcgcgt cgtgctgcgc cgaccggttc
     4921 cgtgcggacg gtggaccgag atcgagagaa ccgacggatg ggtgcgactg ccggccggtg
     4981 tgggactcga cctcggcggc atcgccaagg ggtggacggt cgatctcgcc gtcgagcggg
     5041 cgctggcgtc cggcatgtcg tgggtgctcg tctcggcggg cggcgacctc cgcgtcggcg
     5101 gagacgctcc cccgctggcg atcgatgtcg aggaccccgt cgatccctcg agcgcgatca
     5161 tgaccgtgca gatcgacagg ggtgccctcg cgacctcgag caccgcgaag cgctcgtggg
     5221 gtccgggact ccaccacgtc atcgatccac gcacgggagc gcccgcgatc accgatgccg
     5281 tgcaggtcac ctcgtgggcg cccacctgcg cccaggcgga ggtcgcggca acggtcgcgc
     5341 tgctccgagg cacccgggcc gtcgccgacg caccgagcgt gatcgtcggc cgcgacggca
     5401 ccatcttcca ctcggtcacc tcgtctcgaa gcggcgagcc atcagacgag ccagaagacg
     5461 aggtggccgc atgaacacgt ggatcctgct ccgcgccgcc ggcatcggca gctacctgat
     5521 gctcttcctc tcgatcgcgt ggggattggt ggcgacgacg tccgtggtct ccacacggat
     5581 ctcgaagccc tcggcgaacc tcttccatgc gttcgcggcg acgaccggac tcgcgctgct
     5641 cggcgtgcac ctcgggctcc tgctcatcga cgagttcatg ccgttctcgg tcgcggacct
     5701 caccgtgccg atggcgtcgg agtaccggcc ggtggcgatc acggccggcg tcgtcgcgat
     5761 gtacgcggtc gtggtcatcg tggtgtcgtc gtggctgcgc aagccgctcg gcacggcatg
     5821 gtggcggcgc ctgcacctgc ttgcggtgcc ggccttcgca ctggcgctcg ctcacggcgt
     5881 cttcgccggc accgacaccg agcgaccctg gatgttcgcg atgtacgcgg tcacggggct
     5941 gctcgtcgtg ttcctcacgc tcgttcgggc attcacctac ggctaccggc ctgcgcgccc
     6001 ggcgcccccg gtcagaaccc gacccacgga accggtgacg ccggcgtgat cgtgtcgtcg
     6061 agctcccacg tgaggcgctc gtcgaggccc acgacgccgg gaacggcgcg gacggcgccc
     6121 cagatcgtga gcacgagcga cctgcgttcg acgcggcctt cgaggtgcac cacaccgtcg
     6181 tcgacgagca cgaggatctg atcggcgacg accgtgaggt cgtcggtgag cacccggcag
     6241 acctcggcct tgatgtcatc gtcgtcgcgg aggaactccg cgagcaggtc catgcgcgag
     6301 acgatgccga cgatccgacc ctccaggtcg accactggca aacgcttcac acgacgttca
     6361 tgcatcagcc gggcaacgtc ggcgaccgac gcttgagggc cgaccgtcac cggtggtgcg
     6421 gtcatcaggt cgcgggccac cagcgcctcg gccttcttcc gctcgtcacg acgtgtgtgc
     6481 ccctcgagga agtgcggttc cagcacggat gggtcctcac gcaggatcat gtcggcctcg
     6541 gacaccacgc cttcgatcac accatctgga tcggtcacgg gcagagcgct cacccggtac
     6601 tcgcgcatcg cgcgaaccac ctgtttgaag ggagccgagc ccggcaccac cacgacggtc
     6661 ctcgtcatca cgtcggccac ggtgcgtgac atcgtcgaca cctcctcgcg gggatgcgga
     6721 gccgttcggc ctcgaacccc acggtcaccg taggtccgac gctcgctcgc gccccgggtc
     6781 cggtgccccc caacgggcgg gacggatcgg ccccgttgct aggctcggga tgtgagcgat
     6841 cgcgagcagg cccccagcgt gcctcccgac gagacgcgaa cgcgcatgtt gatcgaggcg
     6901 gggctcgccc tctcctcaga gctcgacctc gactccgtgc tcgagcgcat cgtggagctg
     6961 gcgctgggca tcaccggggc tcgctacgga gcgctcggcg tgctgggcga ggacgcaccc
     7021 cgcatcgagc gattcatcac ccaaggggtg accgacgagg agcgagccgc gatcggccac
     7081 cttcccgtcg gtcacggcat cctggggctc ctgatcgagg aacgacggcc gatgcgtatc
     7141 cccgagatcg gcgcagaccc gaggtcggcc ggcttcccac cgaaccaccc gccgatgcac
     7201 tcgttcctcg gtgcaccggt caaggcgttg ggccagatct tcggcaacat ctacctcacc
     7261 gagaagcagg gggcgcctga gttcaccgac gacgacgagg cggcgctggt cgtgctggcg
     7321 acacaggccg gcgtcgcgat cgagaacgcc cggctgtacg acgagatgcg ccgcgcccac
     7381 gaggagctcg cgcgtctcga gctgctcgag gagcgcgagc gcatcgccaa ggagctgcac
     7441 gacggggtga tccaggcgtt gttcgcggtg ggcatggggt tgcaaggcac cgcagcgatg
     7501 gccaacgacc ccgagatcgc ccggcgcatc gagaacgcga ccgaagaggt cgaccgcgcg
     7561 atccgcgacc tgcgcaacta catcttcggg ctgcggccgg ggatcctggc cgatcgccag
     7621 ctcgaccaag cattgcagca gctcgcgctc gagttccagg agcgcaccga cgtgctcacg
     7681 atcgtcgacg tcgacgggag cgtggcggcc gagctggcga gccgcgccgc cgacgtggtg
     7741 caactcgtgc gcgaaggcct gtcgaacgtc ggccgccatg cccaggccac gtcgtgccgt
     7801 atcacgctgc ggcgctcgaa cggcggcgcc gaactcgaga tcgacgacga cggtctcggg
     7861 ttcgacccct cgtcgccgtc ctcggggatg ggcatgccga acctgcgcga acgggtgacc
     7921 tcgctgggcg gagagctcac gatcctcagc gtcgccggcg agggcacgac gatcagggct
     7981 caactccccc tctaggcggg cacgagggcc ggggctcgaa ccccggccct cacacggccc
     8041 ccgtcggatc agagactcac tgcacgacga gcgcggtcac catgccgaac atgccaccgg
     8101 gtccctcggc gtgcggcagg atgtggcagt ggaatgccca cgcaccggga tagtcggcct
     8161 tgaccatgat gtcgaacctc gaccccggcg cgatcacgag cgtgtccgcc aggtaccggt
     8221 tctgcttggc caacgggtag ccgtcctccc ccacgaccag gaagtggaag ccgtgcaggt
     8281 gcatcgggtg aagcagggca ccgtcgttcg caaggtggat caggacccag tcgcccttgg
     8341 acgccacgat cggctgtgtg gccgggaacg acttcccgtt caggttgtac ccgagcgtgc
     8401 cgtcgccgac gaacatcgtg tactcgacgg ccgggtcggc gccgtacacc gcggtccagt
     8461 tcgcgttctt cggctccacg atcagcgagc cgtacaaccc cttgtcgacc tgctccgccg
     8521 aattgaagtg cgagtgatag acgtacatgc cgggtgggtc cttgatcgtg aactcgtagg
     8581 tccagtactc gccaggcatg atcggcggct gggtgacgta ggggacgccg tcctcgctgt
     8641 tcggaacggt cagaccgtgg aagtgcagca cgaagggctc ggtcatctgg ttctggacga
     8701 cgaaccgcac gcggtcgccc tcgttgaccc gcacctcggg cccgggcacc tgtccgttga
     8761 acgccatcgc gtcgacgaac tcacccttgg cgatctccca ctggatctcc tcgaccgtga
     8821 agttgtagac cttcacgccg ccgtccatct cgggctccat cggctggttg ccgtaggagt
     8881 cggtctcctt gccggccagg aagtcctgca cgccttcctc gtgacccgcg gccatctcct
     8941 cggcggtcat ggcggcgtgc gaggtcgcat cggtcccccc accgctcgca gccgtcgtcc
     9001 cctcggtggc accatccgac gccgtcaggg ttccgaccat gccgagctgg tcgtgtccgg
     9061 gcaccgtgca cagcgtgtcg tacgtgcccg ccgccagagc aggcacctcg agcgtggtcg
     9121 acccgtccac gtcgatgctc ggcgtctcga tcgtcccgcc gttcacgacg acgccgaacg
     9181 tgtgcgggga ctgaccgtgg ttcatcacgt tgaacgtgat gtcgctgccc gcgggcacct
     9241 cgatcgcggc cggttcgatc gcgaagtcgg acaacatcac gtcgacggtg acgggaccgc
     9301 cggcggccgc cgcctcgccg tcgccggcgg ccggggcctg ctcaccgtcg ccgctgcagg
     9361 cgacggcgac gagcgcgagc accgtcgcca gtgccagcgc tcccatgcgg aaccttcgca
     9421 tcatcgatct ccttccggaa cggacgttcg tgcgtacatg aggggggagt ctgggggccc
     9481 cgccgggccg atgggggtgc caggagaccc cacccggccg ggacctgtgt ccctgcgtcg
     9541 agaccaccgg taccgcgaca ctgggt
//
LOCUS       DATDBZ010000006         3050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_614903, whole genome shotgun sequence.
ACCESSION   DATDBZ010000006 DATDBZ010000000
VERSION     DATDBZ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614903"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VIX62_00120"
     CDS             complement(<1..183)
                     /locus_tag="VIX62_00120"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEY5858673.1"
                     /translation="MLNPRGSKVPAQDSDFLTSAQGQRLPHTDDSLTAGARGPTLLED
                     FHLREKIMHFDHERIPE"
     gene            394..759
                     /locus_tag="VIX62_00125"
     CDS             394..759
                     /locus_tag="VIX62_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEY5858674.1"
                     /translation="MDRFQYLILMGLCVLITLPLEFVFGARVWRRPKRLAIALLPALA
                     LFVAWDLWASATGTWGFNRAYTIGIELPGGMAIEELVFFVVIPTCGLLTIEAVRNILS
                     GRVRLRPLPVREQASSSRR"
     gene            756..1067
                     /locus_tag="VIX62_00130"
     CDS             756..1067
                     /locus_tag="VIX62_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HEY5858675.1"
                     /translation="MMPIYPLLAIVAAVAVLLLELTVFRSGLFRQRAYWVSMAIVFAF
                     MIPVDGWLTKLSAPIVVYREEDTSGIRPVWDIPLEEFAYAFALLTLVILVWDRTGREA
                     S"
     gene            1064..1555
                     /locus_tag="VIX62_00135"
     CDS             1064..1555
                     /locus_tag="VIX62_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY5858676.1"
                     /translation="MTALVIVVVAFAAMEVVSYASHRWLMHGPGMAWHRSHHHPPAGR
                     FEKNDLFPLCFSTLGVAAFAVAALSERLLVFRWIGLGMTLYGVTYLFVHEIVIHGRVP
                     LRIPARGYAKWLSDSHRIHHLFGGEPYGMLMPFVARSLRERAGSATRAPLDRSTRETR
                     IRL"
     gene            complement(1447..1992)
                     /locus_tag="VIX62_00140"
     CDS             complement(1447..1992)
                     /locus_tag="VIX62_00140"
                     /inference="COORDINATES: protein motif:HMM:NF040142.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858677.1"
                     /translation="MPLVHMSNPVVVAVNVGAWAVIHSASGYAVHRLPVERLSRDGWL
                     FRKRAVEREGRLYVETLRIKRWKDRVPEAGALFAGGISKRHLTDDATGGLERFAVETR
                     RAELGHWLAMAPAPLFAIWNPPLGVALMVAYGVGVNLPFIAIQRYNRIRVSRVLRSRG
                     ARVALPARSRNERATNGMSMP"
     gene            complement(1994..>3050)
                     /locus_tag="VIX62_00145"
     CDS             complement(1994..>3050)
                     /locus_tag="VIX62_00145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5858678.1"
                     /translation="VEDAAPCDGLEADAGRVTVVVPARNEAATLPILLATLRALDPAP
                     REVIVVDDGSEDDTAGVASAGGAVVIVAASPPEGWLGKPWACHTGALAATGSHILFLD
                     ADVRLAPSALGALVAVNADRGGLISVQPYHLTSAPYEELSAYFNAVALMGTGAFAVTN
                     LATSAVAFGPCLFTAADDYHRVGGHAAVRAEIIEDIHLARRYRDAGLPVTCGTGGALV
                     RFRMYPGGLRQLVEGWSKNVAAGASAAGRIAVVGAVLWVASHAAVATATVVGVVGWAR
                     GASGVPVIPVVAYLAVAIHQWWVLRRIGSFRWTSAAVFPVPLAAFIAIFLRSVVLTRL
                     RHEVTWRGRRIRVHQTR"
BASE COUNT          459 a         1050 c         1034 g          507 t
ORIGIN      
        1 ctccgggatc cgctcgtgat cgaagtgcat gatcttctcg cggagatgga agtcctccag
       61 caacgtcggc ccgcgcgcac cggcggtcaa cgagtcgtca gtgtggggca ggcgctggcc
      121 ctgagccgaa gtcaggaagt cgctgtcctg agcgggaact ttgctccctc gcgggttcag
      181 gatgggcggc tctgccgttc gagcgacaag gcggcctttg gctccctgct gatccttctt
      241 cgttgcccga gggtcgactc gcgctcgctt gctcgtggcc actgatcgct cctccgctga
      301 ttggttggca cgaggtaccc agcgctcgat gaggcgaaac gcggcccgcc ggattcccgt
      361 tcgtagactc accgtgaacg gaggtgtggc cgagtggatc ggttccagta tctgatcttg
      421 atgggtctgt gcgtcctgat cacgctgccg ctcgagttcg ttttcggggc gcgggtgtgg
      481 cgccgcccga agcgtctcgc gatcgcgctc ctgcccgcgc tggcgctctt cgtcgcctgg
      541 gacctgtggg cgtcggcgac gggaacgtgg ggcttcaacc gcgcctacac catcggcatc
      601 gagctgccgg gcggcatggc gatcgaagag ctcgtgttct tcgtggtcat ccccacctgc
      661 gggctgctca cgatcgaggc ggtgcgcaac atcctgtccg gccgtgttcg gttgcgtccc
      721 ctgccggtcc gcgaacaggc atcgagctcg cgtcgatgat gccgatctac cccctgctcg
      781 ccatcgtcgc cgcggtggcc gtgctcctgc tcgagttgac ggtgttccgc tcggggctgt
      841 tccgccagcg cgcctactgg gtgtcgatgg cgatcgtgtt cgcgttcatg atcccggtcg
      901 acggttggct gacgaagctg tcggcgccga tcgtcgtcta ccgcgaagag gacacgagcg
      961 gcatccgacc ggtttgggac atcccgctcg aggagttcgc gtatgcgttc gcactcctca
     1021 ccctggtgat cctggtatgg gatcggaccg gacgggaggc gtcatgactg cgttggtgat
     1081 cgtcgtcgtc gcattcgcgg ccatggaggt ggtgtcctac gcctcgcacc gttggctgat
     1141 gcacggtcct ggcatggcat ggcatcgcag ccaccaccat ccgcccgccg gacggttcga
     1201 gaagaacgac ctgttcccgc tctgcttctc cacgctcggc gtggccgcct tcgccgttgc
     1261 ggcactgtcg gagcggctgc tggtgttccg ctggatcgga ctggggatga cgctctacgg
     1321 cgtcacgtac ctcttcgtgc acgaaatcgt gatccacggc cgtgtgccac tcaggatccc
     1381 ggcccggggc tacgccaaat ggttgagcga ctcgcatcgc atccaccacc tcttcggggg
     1441 cgagccctac ggcatgctca tgccgttcgt tgcccgttcg ttgcgcgagc gcgccggtag
     1501 cgcgactcgt gcccctctcg accggagcac gcgcgagacg cggatccggt tgtaacgctg
     1561 gatggcgatg aacggcaggt tgaccccgac gccgtaggca accatcagcg cgacaccgag
     1621 gggcgggttc cagatcgcga acagtggggc gggcgccatg gccaaccagt ggccgagctc
     1681 ggcccggcgg gtctcgacgg cgaatcgctc caagccgccg gtcgcgtcgt cggtcagatg
     1741 ccgcttcgag atgccgccgg cgaacagcgc gccggcctcc ggcacccggt ccttccatcg
     1801 cttgatccgc agcgtctcga cgtagagccg tccctcgcgc tcgaccgccc gcttccggaa
     1861 caaccagccg tcgcgcgaca accgctcgac agggagccga tgcacggcat accccgacgc
     1921 cgaatggatc actgcccagg ccccgacgtt gacggcgacg accaccggat tggacatgtg
     1981 gacgagcggc acgtcagcga gtctggtgca cgcggatccg tcgcccgcgc cacgtgacct
     2041 cgtggcggag ccgcgtcagg accaccgacc gcaggaagat cgcgatgaat gccgccaatg
     2101 gcaccgggaa gacggctgcg ctcgtccagc ggaacgaacc gatgcggcgg agcacccacc
     2161 actggtggat ggcgacggcg agatacgcga cgaccgggat gacgggtacc ccggacgcac
     2221 cgcgtgccca accgacgaca ccaaccacgg tggctgtggc aaccgcagcg tgcgacgcga
     2281 cccagagcac cgccccgacc accgcgatcc gtcccgctgc cgacgcgccg gcagccacgt
     2341 tcttgctcca cccctccacg agctggcgga ggccgcctgg gtacatcctg aacctcacga
     2401 gcgcgccgcc cgtgccacac gtgaccggga gacccgcgtc ccggtaccga cgagcgaggt
     2461 ggatgtcctc gatgatctcg gctcgcacgg cggcgtggcc gcccactcgg tggtagtcgt
     2521 cggccgccgt gaacaggcat ggcccgaagg cgaccgctga ggtcgcgagg tttgtcaccg
     2581 cgaaggcgcc ggtgcccatc aatgcaacgg cgttgaagta cgccgacagc tcctcgtacg
     2641 gtgccgacgt gaggtggtac ggctggacgg agatcagccc accccggtcg gcgttgacgg
     2701 cgacgagcgc gccgagcgcc gacggcgcca gccggacgtc ggcatcgagg aacaggatgt
     2761 gcgaacccgt ggcggcgagc gctcccgtgt ggcacgccca cggtttgccc agccagccct
     2821 ccggcggcga cgccgcgacg atgacgaccg cgccgccggc gctggcaacg cccgcagtgt
     2881 cgtcctcgga tccgtcatcg acgacgatga cctcccgcgg ggcgggatcg agggcccgga
     2941 gcgtcgcgag caggatcggc aacgtggctg cttcgttacg agccggaacg acgacggtca
     3001 cccggccggc gtcggcctcg agcccgtcgc agggggccgc gtcttcgaca
//
LOCUS       DATDBZ010000007         5309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2865611, whole genome shotgun sequence.
ACCESSION   DATDBZ010000007 DATDBZ010000000
VERSION     DATDBZ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2865611"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VIX62_00150"
     CDS             complement(<1..696)
                     /locus_tag="VIX62_00150"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5858679.1"
                     /translation="MSPVAVTTLSGPGGEVHIGDEHPFAIIGERINPTGRSVLTEQMK
                     AGDMTTVQADAIAQVQAGAAVLDLNAGIPGFDEPAMLSQVVKAVHEVVDVPLCIDSST
                     PEALEAAVPLAQGKVLINSVTAEAHSLERLLPLVKKFGAAVIGMANDEEGISMDPQER
                     LAAARKVVEAADDHGIAREDVIIDPLTMPIGAAGDAATAMFETVRLIRDELGVNVSCG
                     ASNISFGMPDRQGI"
     gene            complement(693..1346)
                     /locus_tag="VIX62_00155"
     CDS             complement(693..1346)
                     /locus_tag="VIX62_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY5858680.1"
                     /translation="MQEDLEVLKRALIAADMDLGVAAVQRLVDHDADPKWILEQGMAV
                     AMFDLGEMWKRGEVFLPEVVASAEVFKACNAIVEPVLLAQKDPNAEANALVLLATVKG
                     DLHDLGKNMVGAMLRTSGFDVHDLGKDVSADTIIAAIEEMQPKIIGLSALLTTTVPYQ
                     ETVIKKLVAAGLRDKVIVMVGGAPVTPEWADKIGADGYANNAPEAVEIAKKLVGVAV"
     gene            1526..2191
                     /locus_tag="VIX62_00160"
     CDS             1526..2191
                     /locus_tag="VIX62_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5858681.1"
                     /translation="MATTPTLGSRSERTQAKRERIVECAMRHFAEQGYHGAKVEDIAR
                     ELGIAKGSIFQHFKSKAGLFFEAYKRAATSLPAWLDVPDDVKAEGFFGVVTFWLERTE
                     HLIKEDWVPNRVILIGNYGTDLELKRDINRFLVSEDPYGTLEFVEWGQERGEVRADVD
                     LEMLVSMVDWLSNSLQDALVTEELDPGLFHRLRTQPERQQQRVVDFTTLLASAIGASP
                     RSV"
     gene            complement(2188..2364)
                     /locus_tag="VIX62_00165"
     CDS             complement(2188..2364)
                     /locus_tag="VIX62_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858682.1"
                     /translation="MIDFIEDNHDLIMGAALMIAGVLTVLAIRKPSKRVKTFALVGAA
                     VTFVIGALLFLGLA"
     gene            complement(2446..2940)
                     /locus_tag="VIX62_00170"
     CDS             complement(2446..2940)
                     /locus_tag="VIX62_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858683.1"
                     /translation="MPASRITTTVHGLTPERVRHALNGLPRADGYALHVKPLRYRQRP
                     HLSAWTDFDERSITLQVPEPFFPFGEIVPYAAKRRPSARKGGPPKFIWLTEGVTFRTP
                     REVLRFLYCHEWMHVYGTVREGKRRVGETACDRFALQNYRRRTVTIEDAEASLLPISA
                     APKG"
     gene            3048..3749
                     /locus_tag="VIX62_00175"
     CDS             3048..3749
                     /locus_tag="VIX62_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY5858684.1"
                     /translation="MSEPARERDHRDGARAVAPLAIAVLGFGASYGVLARAAGMGVLA
                     PIVMSATTFAGSAQFAAASILGTGGATLSAITAAILLNARYAPIGVSVAPYLTGGFWS
                     RFLHAQLTVDETWAVAAEGEGRFNPKVLLGAGLILYAAWVLGTILGVAFGDVIGDPAN
                     WGLDAAFPALFLALLVPQLKDRRTLEAALLGAAIALALTPFVPAGVPIIAASAACLLG
                     LRETPPAPPDPGTTP"
     gene            3776..4063
                     /locus_tag="VIX62_00180"
     CDS             3776..4063
                     /locus_tag="VIX62_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY5858685.1"
                     /translation="MVVGIATIAIKALGPVLLGGRPLPPRLAGPIALLAPALLAALVA
                     INTFGADRQLVLDARVPGVAAAAVAIWLKAPVLVVIIVAAAVTAVVRAVAG"
     gene            4075..5223
                     /locus_tag="VIX62_00185"
     CDS             4075..5223
                     /locus_tag="VIX62_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY5858686.1"
                     /translation="MATSPPRYRDLGLTVGMLDPGPNNAITDVDGVTVGVTTLIEGEG
                     PLVPGKGPVRTGVTAILPHADIGAEPVFAGCHRLNGNGELTGLEWVRESGQLTTPIAI
                     TNTHSVGVVRDAIVAADMRARSDIGAFWSLPVVGETWDGTLNDINGFHVKPEHVHHAL
                     ETATTGPVPEGNVGGGTAMICHGFKGGTGTASRTLSFDEGGWTVGALVQANYGRRERL
                     TVDGVPVGREIGPDVVPTPYDESPTSPPPGAGSIIVILATDAPLLPHQCERIAQRAGL
                     GIARVGGAGEHSSGDLFLAFSTANRGRLASYKDDIAAGPFNVTMLPDAAISPLFWAAI
                     EATEEAILNALIAAETLTGIDGVTAHALPHDTLVDVMARYGRGPQPRV"
BASE COUNT          778 a         1879 c         1805 g          847 t
ORIGIN      
        1 gatcccctgg cggtcgggca tgccgaacga gatgttcgag gccccgcagc tcacgttgac
       61 gccgagctca tcgcggatca gccgaaccgt ctcgaacatc gccgtggcgg cgtcgcccgc
      121 cgcgccgatg ggcatcgtga gcggatcgat gatgacgtcc tcgcgagcga tgccgtggtc
      181 gtcggcggct tccaccacct tgcgcgcggc ggcgaggcgt tcctgcgggt ccatggagat
      241 gccctcttcg tcgttggcca tgccgatcac cgcggcgccg aacttcttga ccagcggcag
      301 caggcgttcg agcgaatggg cctcggcggt cacggagttg atcagcacct tgccctgcgc
      361 gagcggcacg gccgcctcga gcgcttcggg ggtggacgag tcgatgcaga gcggcacgtc
      421 gacgacctcg tgcacggcct tgaccacctg cgagagcatc gccggttcgt cgaagccggg
      481 gatccccgcg ttgaggtcga gcaccgcggc gccggcctgg acctgggcga tcgcatcggc
      541 ctggaccgtg gtcatgtcgc cggccttcat ctgttcggtg aggaccgagc ggccggtggg
      601 gttgatgcgt tcgccgatga tcgcgaacgg gtgctcgtcc ccgatgtgga cctcgccacc
      661 gggaccggac agcgtggtga ccgccaccgg gctcatacgg cgacgcccac cagcttcttc
      721 gcgatctcga ccgcctcggg cgcgttgttc gcgtagccgt cggcgccgat cttgtcggcc
      781 cactcggggg tgacgggcgc gccgcccacc atgacgatga ccttgtcgcg caggccggcg
      841 gccacgagct tcttgatcac cgtctcctgg tagggaaccg tggtggtgag cagggccgag
      901 aggccgatga tcttcggctg catctcctcg atggccgcga tgatcgtgtc ggcgctgacg
      961 tccttgccca ggtcgtgcac atcgaagccg ctcgtccgca gcatggcgcc caccatgttc
     1021 ttgcccaggt cgtgcaggtc gcccttcacg gtggcgagga gcacgagggc gttcgcttcg
     1081 gcgttcggat ccttctgggc gagcagcacg ggttcgacga tggcgttgca ggccttgaac
     1141 acctcggcgc tggccaccac ctcgggcagg aacacctcgc cgcgcttcca catctctccc
     1201 aggtcgaaca tggccaccgc catgccctgc tccaggatcc acttggggtc ggcgtcgtgg
     1261 tcgaccaggc gttggacggc cgccacgccg agatccatgt cggcggcgat cagcgcccgt
     1321 ttcagaacct cgaggtcttc ctgcatcgtc gcgtccttcg tggaggccgg tggtgcgggc
     1381 tcgctgacta ccagtcatgg catcgccggg aggcgggttc tcccgatgat gccaggtcat
     1441 cggcatcggc gggagtcctc atcagccttg ccgcgtgacc acgagtcatg caagacttcg
     1501 cgcactctct accctttgga gcgtcatggc aacgaccccc accctcggca gccgaagtga
     1561 gcgcacgcaa gccaagcgcg agcgcatcgt ggagtgcgcg atgcgccatt tcgccgagca
     1621 gggctaccac ggcgccaagg tcgaggacat cgcgcgcgag ctgggcatcg cgaaaggttc
     1681 gatcttccag cacttcaagt cgaaggccgg gctgttcttc gaggcctaca agcgggcggc
     1741 gacctcgctc cccgcctggc tcgacgtccc cgatgacgtc aaggcggagg ggttcttcgg
     1801 cgtggtgacg ttctggctcg agcggaccga gcacctgatc aaggaggact gggtgcccaa
     1861 ccgggtgatc ctgatcggga actacggcac cgacctggag ctgaagcgcg acatcaaccg
     1921 cttcctggtg agcgaggacc cctacggcac gctggagttc gtggaatggg gtcaggagcg
     1981 aggtgaagtg cgggccgacg tcgacctgga gatgctggtg tcgatggtcg actggctgtc
     2041 gaactcgctt caggacgcct tggtgaccga ggagctcgac cccgggctct tccatcgcct
     2101 gcggacccag cccgagcgac agcagcagcg ggtcgtggac ttcacgacgc tgttggcgag
     2161 cgcgatcggc gcgtcgcccc gctcggtcta ggcgagcccc aggaacagca gcgcgccgat
     2221 cacgaacgtg acggcggcgc ccaccagcgc gaaggtcttc acccgcttgg acggtttcct
     2281 gatcgcgagg accgtcagca cgccggcgat catcagggcc gcccccatga tcaggtcgtg
     2341 gttgtcctcg atgaagtcga tcacgccatc tccctccgtc gccgcgtcca gtcagaagcc
     2401 gcaagcagcg tagaccatgc caggcggcgg ctggagccgt cggcgctagc ccttcggagc
     2461 cgcgctgatg ggcaggagtg aggcttcagc gtcctcgatc gtgaccgtcc tccggcggta
     2521 gttctgcagc gcgaagcggt cgcatgcggt ttctccgact cggcgcttcc cctcgcggac
     2581 ggtcccgtag acgtgcatcc actcgtggca gtagaggaac cgaaggacct cgcgtggcgt
     2641 gcggaacgtg acgccctcgg tgagccagat gaacttgggc gggccaccct tgcgggccga
     2701 cggtcgccgt ttggcggcgt aggggacgat ctccccgaac gggaagaacg gttcggggac
     2761 ctgcaacgtg atggagcgct cgtcgaagtc ggtccatgcc gacaggtgag ggcgttgccg
     2821 gtagcgaagc ggcttgacgt gaagggcata cccgtcggcg cgcggaaggc cgttcagggc
     2881 gtgccggacg cgttcgggcg tgaggccatg gaccgtcgtg gtgatcctgg atgcgggcac
     2941 gagcagaggg tagcggcggc caccgacagg cagcgaggtt cccccggcga aggatggcaa
     3001 ggaccccacg gatcgcatat ccacctgccc gtagcatgag cagcaccatg tccgagcccg
     3061 cccgtgagcg agaccaccgc gatggcgcgc gcgccgtcgc gcccctcgcc atcgccgtgc
     3121 tcgggttcgg cgcctcctac ggcgtcctcg cgcgcgccgc gggcatgggc gtcctcgcgc
     3181 ccatcgtgat gtcggccacc accttcgccg ggtccgccca gttcgccgcc gcctcgatct
     3241 tgggcaccgg cggcgccacc ctctcggcca tcaccgccgc tatcctgctc aacgcccggt
     3301 acgccccgat cggcgtcagc gtcgcgccct acctcaccgg tggcttctgg tcccgcttcc
     3361 tgcacgccca gctcaccgtc gatgagacat gggcggtggc cgccgagggc gagggccgct
     3421 tcaatccgaa ggtcctgctc ggcgccgggc tgatcctcta cgccgcctgg gtgctcggca
     3481 ccatcctggg tgtcgcgttc ggcgacgtca tcggggaccc cgcgaattgg ggcttggacg
     3541 ccgccttccc cgccctcttc ctggcgctcc tggtccccca actcaaggac cgcaggaccc
     3601 tcgaagcggc tctgctcggc gccgcgatcg ccctcgcgct cacgccgttc gtaccggccg
     3661 gtgtcccgat catcgcagcc agcgccgcct gtctcctggg tctccgcgaa acgccgccgg
     3721 caccccccga ccccgggaca accccatgag cgtgagcacc gcctggaccg tcgtcatcgt
     3781 cgtggggatc gccacgatcg ccatcaaggc gctcgggccg gtcctgctcg gtgggcgccc
     3841 gctcccgccg aggctcgcgg ggccgatcgc cctgctcgcg cccgcgttgc tggccgccct
     3901 ggtcgcgatc aacacgttcg gcgccgaccg ccagttggtg ctcgacgccc gcgtcccggg
     3961 cgtggccgcc gccgccgtcg cgatctggtt gaaagccccc gtcctggtgg tgatcatcgt
     4021 ggccgcggcg gtgaccgccg tggtgcgcgc ggtcgccggg taggctctgc ggccatggcg
     4081 accagcccac cccgctaccg ggacctcggt ctcaccgtcg ggatgctcga cccgggaccg
     4141 aacaacgcga tcaccgacgt tgacggcgtc accgtcggcg tcacgacctt gatcgagggc
     4201 gagggtccgc tcgttccggg caagggaccc gttcgcaccg gggtcacggc gatcctgccg
     4261 cacgccgaca tcggcgccga gcccgtcttc gccgggtgtc atcgcctgaa tggcaatggc
     4321 gagctcaccg gtctcgaatg ggtgcgcgaa tcggggcagc tcaccacccc gatcgccatc
     4381 accaacaccc acagcgtggg cgtggtgcgg gacgccatcg tggcggccga catgcgggcg
     4441 cggtccgata tcggcgcctt ctggagcctg ccggtggtgg gcgagacgtg ggacggcacc
     4501 ctcaacgaca tcaacgggtt ccacgtgaag cccgagcacg tccaccacgc actcgagacg
     4561 gcgaccaccg ggcccgtccc cgagggcaac gtgggcggcg gcaccgcgat gatctgccac
     4621 ggcttcaagg gcggaaccgg cacggcctca cgaacgctgt ccttcgacga gggtggctgg
     4681 accgtgggcg ccctcgtgca agcgaactac ggccgccgcg agcgcctcac ggtcgacggg
     4741 gttcccgtgg gccgcgagat cggacccgat gtcgtgccta ccccgtacga cgagtccccc
     4801 acgagcccgc cgccgggcgc tggatcgatc atcgtgatcc tggcgaccga cgcgccgcta
     4861 ctcccccacc agtgcgagcg catcgcgcaa cgtgccgggc tggggatcgc gcgcgtcggc
     4921 ggagcaggcg aacactcgag cggcgacctg ttcctcgcgt tctccaccgc gaaccgcggt
     4981 cggctggcca gctacaagga cgacatcgcc gccgggccgt tcaacgtcac gatgctgccc
     5041 gacgccgcga tcagcccgct cttctgggct gcgatcgagg ccaccgagga agcgatcctg
     5101 aatgcgctga tcgccgccga aacactcacc gggatcgacg gcgtcaccgc gcacgccctg
     5161 ccgcacgaca cgctggtcga cgtgatggcc cgctacgggc gcgggccgca gccgcgcgtc
     5221 tagcgcgact cgcccgggca gcgacggtcc aggtcggtcg ccgtcggccc cgcgatctgc
     5281 accaccgtgg aacgaccgtt gtcggtctc
//
LOCUS       DATDBZ010000008         6819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_2097,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000008 DATDBZ010000000
VERSION     DATDBZ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VIX62_00190"
     CDS             complement(<1..554)
                     /locus_tag="VIX62_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016476825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5858687.1"
                     /translation="MDDDRLIEAAVVDPEDREFDVALRPRSLTEFVGQERVKEQLALL
                     IDGARRRSEPVDHLLFSGPPGLGKTTLAQIVATEMAAGFNPTSGPALDRPSDLAAILT
                     NLGDGDVLFVDEIHRMPRPVEEVLYPALEDFVLDVVLGKGPTARSIRLELPRFTLVAA
                     TTRPGRITLPLRERFGFSPRLDYYS"
     gene            complement(556..1122)
                     /gene="ruvA"
                     /locus_tag="VIX62_00195"
     CDS             complement(556..1122)
                     /gene="ruvA"
                     /locus_tag="VIX62_00195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY5858688.1"
                     /translation="MISFLEGEVVEKGANRIVLAVGGVGYDLLVPTSVVSKLPPTGKT
                     TRVHTRMVVRDDAMVLYGFSATDERELFDLLTGVSGVGPKVALSFLSALAPDALRRSV
                     VAGDAEALTVVPGVGKKVAQRVVLDLRDKLGGEGELVAEGPLADVREALVSLGLTPAE
                     ASEALASVQIDDREVEDLLREALQKAGR"
     gene            complement(1119..1631)
                     /gene="ruvC"
                     /locus_tag="VIX62_00200"
     CDS             complement(1119..1631)
                     /gene="ruvC"
                     /locus_tag="VIX62_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY5858689.1"
                     /translation="MFDTCVLGVDPGVARLGLAVVGREGRKPALVWADTIRTPSIQDE
                     SARLLHLVTAMRTAIAQHAPTCVAIERVAFSRNRSSALTVARVTGAVMVVAAEAGLPV
                     AEYSPTQVKSAVTGIGNADKDQVRTALIRVHAMREVPDQPDAADAIAVALTHLTGERL
                     RTAARAVGAR"
     gene            complement(1699..2448)
                     /locus_tag="VIX62_00205"
     CDS             complement(1699..2448)
                     /locus_tag="VIX62_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5858690.1"
                     /translation="MSGHSKWATIKRKKEATDAKRSNMYAKLLRAVEVAAREGGGNVE
                     GNMTLASAVEKAKSFSVPNDNIDRAIKRGTGDLGDGARYEELLYEGYAPGGVALLIEA
                     LTDNRNRTGQEVRHAFTRNNGNMGEQGSTGWMFTRKGLIVVEKSVAPDEERLLELALE
                     SGAEDLSDSESSWDIVTEPGQFKVVQQALEGAGVPVFSAEITMIPQNTIPVEGGEAKQ
                     VLGLIEALEELDDVQNVYANFDIPEEVMASL"
     gene            complement(2458..3024)
                     /gene="pdxT"
                     /locus_tag="VIX62_00210"
     CDS             complement(2458..3024)
                     /gene="pdxT"
                     /locus_tag="VIX62_00210"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEY5858691.1"
                     /translation="MTTVKAGVLALQGDFREHGRMLAEAGATPVEVRNADDLAEVGCL
                     VIPGGESTTMAKLARAYDLVEPVRARAARGMPIFGTCAGMIVLARRVEGGDPLFAVMD
                     IGVQRNAYGRQVDSFEDQVDVEGIDHAVRGVFIRAPKITDVGADVRILASHEGDPVVL
                     EQGNLLVSSFHPELIGETGLHRYLLSKM"
     gene            complement(3034..3915)
                     /gene="pdxS"
                     /locus_tag="VIX62_00215"
     CDS             complement(3034..3915)
                     /gene="pdxS"
                     /locus_tag="VIX62_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEY5858692.1"
                     /translation="MQRETGTFTVKSGLAEMLKGGVIMDVTTADQAKIAEDAGACAVM
                     ALERVPADIRRDGGVARMADATKVEEIQQVVSIPVMAKARIGHFVEAQVLQALGVDYV
                     DESEVLTPADEEYHIDKWAFTVPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVVE
                     AVRHERQILRDIAEVTTLRPEQYVTKAKELGAPVELVRMVAEQGKLPVVNFSAGGIAT
                     PADAALMMQLGVDGVFVGSGIFKSQDPATMARAVVEATTNFENPDVLAKLSHGLGDPM
                     AGLEISKLETRLQDRGW"
     gene            complement(4000..5055)
                     /locus_tag="VIX62_00220"
     CDS             complement(4000..5055)
                     /locus_tag="VIX62_00220"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY5858693.1"
                     /translation="MALTTRGKALIAGGAVVAVLGGGVAALALNGDNPVAKVLDPVVP
                     GDQTPPTPCPLTNQPVGEDKEPPARPALAIKVENTPDAMPLVGLDKADVVYEEVVEGG
                     ITRFIVVFNCSSAERVGPVRSARTTDPKILLQFSPHPLLAYSGGANRVVKIIKESGVV
                     GMTEGDPAPPFTRDDGREVPHNLFVNTNKLWVAGQKRAKDEPVPEAIFSYGDLNKPSK
                     KTTSATIAFPLTDVEWRWAGGRWVRYQNGEEMLLENGDAIETDNVLIQQVKTSESDIV
                     DVVGYPSPEVEVTGAGKAWLLRDGKLVIGTWERNSEGDITVFRTKKGDEFQLKPGTTF
                     VELAPTGMFTAPVTFGS"
     gene            complement(5211..6116)
                     /locus_tag="VIX62_00225"
     CDS             complement(5211..6116)
                     /locus_tag="VIX62_00225"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5858694.1"
                     /translation="MYEEIASNKRKSVLLVIGAFLFLGVIGLAIGYLFGTGPLGLAIA
                     LVVAFLLATWSYFSGDKVVLASTRAKLVTPDEQPRLHNIVEGIAIAAGVPKPKVYVVP
                     EQAPNAYATGRDPEHSSIAVTEGLLTMMNRVELEGVIAHEMSHVVDRDILLGTIVATL
                     VGAAVLLSEFFMRSWWWGGIGGRRGNDSNGGGFGLIMFAVGFALMAIAPLAGQVIKLS
                     VSRNREYLADAQGALLTRYPPGLISALRKLETAPTAMRSANNATAHLWLNQPSRIPGE
                     KNSTMERLFSTHPPLAERIRRLEEM"
     gene            complement(6117..6683)
                     /locus_tag="VIX62_00230"
     CDS             complement(6117..6683)
                     /locus_tag="VIX62_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY5858695.1"
                     /translation="MSPVLWIVVGLLAVVLLAGVYLFNRLVALRTRVDNGWSQIDVQL
                     RRRYDLIPNLVETVKGYASHEREVFEHVTEARAGAMAADGVDQQAQAENAITGGLRQL
                     FAVAENYPELKANQNFLALQEELTGTESKIAYARQFYNDQVMKLNTLVGSFPSNLVAK
                     AFGFTTRDFFDIDDPERGPVRVDLTPGT"
BASE COUNT         1041 a         2368 c         2271 g         1139 t
ORIGIN      
        1 gagtagtagt cgagccgcgg cgagaacccg aagcgttccc gcagcggcag cgtgatgcgt
       61 cccggtcgcg tggtggccgc caccagggtg aaccgcggga gttccagcct gatcgaccgc
      121 gccgtcgggc ctttgcccag caccacatcg agcacgaagt cctccagcgc cggatacagc
      181 acttcctcga cgggccgcgg catgcggtgg atctcatcga cgaacaacac gtcgccgtcc
      241 ccgaggttgg tgaggatggc ggcgaggtca ctgggacgat ccagggccgg gccggacgtg
      301 gggttgaacc ccgcggccat ctcggtggcc acgatctggg ccagcgtggt cttgcccagc
      361 cccggtggac cgctgaacag cagatggtcg acgggctcgg agcggcgtct cgcgccgtcg
      421 atcagcagcg ccaactgttc cttcacccgc tcctgaccca cgaactcggt gagcgaacgg
      481 gggcgcagtg cgacgtcgaa ctcccgatcc tccgggtcga cgacggcggc ttcgatcaac
      541 cggtcgtcgt ccatgtcagc gacccgcctt ctgcagggct tcccgcaaca gatcctcgac
      601 ctcgcggtcg tcgatctgca cggacgccag ggcctcgctc gcttcggccg gggtgagacc
      661 caacgacacc aacgcctcgc gcacatcggc gagcgggccc tcggccacga gttctccctc
      721 gccgcccagt ttgtcgcgca ggtcaagcac cacgcgctgc gccaccttct tgcccacccc
      781 cgggacgacc gtgagggcct cggcgtcgcc ggccaccacg ctgcgccgca gcgcatcggg
      841 cgccaacgcc gacaggaagg acagggccac cttgggaccg acgccgctca caccggtcag
      901 caggtcgaac agttcgcgct cgtcggtggc ggagaagccg tacaggacca tggcgtcgtc
      961 ccgcacgacc atgcgcgtgt gcacacgtgt ggtcttgccg gtgggtggga gcttgctcac
     1021 caccgacgtg ggcaccagca ggtcgtagcc gaccccgccg acggccagca cgatccggtt
     1081 cgcccccttc tcgaccacct cgccctccag gaacgagatc atcgcgctcc caccgctcga
     1141 gcggccgtgc gcagccgctc gccggttaga tgggtgaggg ccaccgcgat ggcatcggcc
     1201 gcgtcgggtt ggtccggcac ctcgcgcatc gcgtggacgc ggatgagggc ggtgcggacc
     1261 tggtccttgt cggcgttgcc gatgccggtg accgcgctct tcacctgggt ggggctgtac
     1321 tcggccaccg gcaggcccgc ctcggcggcc accaccatca ccgccccggt gacacgggcg
     1381 acggtgagcg cgctgctccg gttcctgctg aaggcgacgc gctcgatggc cacacaggtg
     1441 ggtgcgtgct gcgcgatcgc cgtgcgcatc gcggtcacca gatgcaggag cctggccgac
     1501 tcgtcctgga tggatggcgt gcggatcgtg tctgcccaga ccagcgcggg cttgcgaccc
     1561 tcacgaccga cgacggcgag gcccaaccgg gccacgccag ggtccacgcc gagcacacag
     1621 gtatcgaaca tccgttcgcc catcctaggg accggcggcg gccggggcaa cgacctcgga
     1681 acgccgtgat gagcctcgtc agagggaggc catgacctct tcggggatgt cgaagttggc
     1741 atagacgttc tgcacgtcgt cgagttcctc gagcgcctcg atcaggccga gcacctgctt
     1801 cgcctcgccg ccctccacgg ggatcgtgtt ctgcgggatc atcgtgatct ccgccgagaa
     1861 caccggaacc cccgcgcctt ccagtgcttg ctgcaccacc ttgaactgcc cgggctcggt
     1921 gacgatgtcc caggagctct cggagtcgga caggtcctcg gcgccggact ccaacgcgag
     1981 ctccaggagg cgttcctcgt cgggcgccac cgacttctcc accacgatca aacccttgcg
     2041 ggtgaacatc caaccggtac tgccctgctc gcccatgttg ccgttgttcc gggtgaaggc
     2101 gtgccgaacc tcctggccgg ttcggttgcg gttgtcggtg agcgcctcga tcaggagggc
     2161 gacgccgccg ggcgcatacc cctcgtagag gagctcctcg tatcgcgcgc cgtccccgag
     2221 gtcaccggta ccccgcttga tcgcgcgatc gatgttgtcg ttcgggacgc tgaagctctt
     2281 cgccttctcg acggcgctgg cgagcgtcat gttgccctcg acgttgccgc cgccctcgcg
     2341 cgccgccacc tccacggcac gcaggagctt cgcgtacatg ttcgaccgct tcgcgtcggt
     2401 cgcttccttc ttccgtttga tcgtcgccca cttggaatgg cccgacatgg tgtcccctca
     2461 catcttcgag agcaggtagc ggtgcagacc ggtctcgccg atcagttccg gatggaacga
     2521 tgatacgagc aggttcccct gctccaacac gacggggtcg ccctcgtgac tggccaggat
     2581 ccgcacgtcc gcgcccacgt ccgtgatctt cggcgcacgg atgaagaccc cccgcaccgc
     2641 atggtcgatg ccttcgacgt ccacttgatc ctcgaacgaa tcgacctgcc ggccgtaggc
     2701 gttgcgctgc acgccgatgt ccatcactgc gaacagggga tcgccgccct cgacgcgccg
     2761 ggcgagcacg atcatgccgg cgcacgtgcc gaagatcggc atgccccggg cggcacgtgc
     2821 ccggacgggc tcgaccaggt cgtacgcgcg tgcgagcttg gccatcgtgg tggattcgcc
     2881 gccggggatc accaggcagc cgacctcggc gagatcgtcg gcgttccgga cctcgaccgg
     2941 cgtggcaccc gcctcggcca gcatgcgacc gtgctcacgg aagtcaccct gcagcgcgag
     3001 gacgcccgct ttcacggtcg tcaacctcct tgactaccag ccgcggtctt gcaggcgggt
     3061 ctccagcttc gagatctcga gcccggccat cgggtcgccc aggccgtggg agagcttcgc
     3121 cagcacgtcg gggttctcga agttcgtggt ggcctcgacg acggcacgcg ccatcgtggc
     3181 ggggtcctgg gacttgaaga tgcctgagcc cacgaacacg ccgtcgaccc ccagctgcat
     3241 catcaacgcg gcgtcggcag gggtggcgat gccaccggca gagaagttca cgacgggcag
     3301 cttgccctgc tcggccacca tgcgcaccag ctcgaccggt gcacccagct ccttggcctt
     3361 cgtgacgtac tgctcgggcc gcagcgtggt gacctcggcg atgtcgcgca ggatctggcg
     3421 ctcgtgccgg accgcctcga cgacgttgcc cgtgccggcc tcgcccttcg tgcggatcat
     3481 cgcagcgccc tcggcgatgc gacgaagcgc ctcacccagg ttccgcgcgc cgcagacgaa
     3541 cggcacggtg aaggcccact tgtcgatgtg gtactcctcg tcggccggcg tcagcacctc
     3601 ggattcgtcg acgtagtcga ccccgagcgc ctggagcacc tgggcctcga cgaaatgccc
     3661 gatgcgcgct ttggccatga cggggatcga gacgacctgc tggatctcct cgaccttcgt
     3721 ggcgtcggcc atgcgggcga ccccgccatc tctgcggatg tcggccggaa cacgctcgag
     3781 ggccatgacg gcgcaggcgc cggcatcctc ggcgatcttc gcctgatcgg ccgtggtgac
     3841 gtccatgatg acgccgccct tgagcatctc ggccaggccg gacttcacgg tgaaggtgcc
     3901 ggtctcgcgc tgcacgggta tcgcctccta cgaagggaac ggtctgcctc cgagtgtacc
     3961 ggaggccacc tcgcagctcg gaggccgaac cgtcgcgcgc taggacccga aggtcaccgg
     4021 agcggtgaac atgccggtgg gcgccagctc gacgaacgtg gtgccgggct tgagctggaa
     4081 ctcatcgccc ttcttcgtgc ggaagacggt gatgtcgccc tcggagttgc gttcccaggt
     4141 gccgatgacg agcttgccgt cgcgcagcaa ccacgccttg ccggcgccgg tgacctccac
     4201 ctcgggcgag ggatagccca ccacgtcgac gatgtcgctc tcggaggttt tcacctgctg
     4261 gatcaggacg ttgtcggtct cgatcgcgtc gccgttctcc aacagcatct cctcgccgtt
     4321 ctggtagcgg acccagcggc ctcccgccca gcgccactcg acgtcggtga gcgggaacgc
     4381 gatcgtcgcg gaggtggtct tcttgctggg cttgttgagg tcgccgtagc tgaagatcgc
     4441 ctcgggcacg ggctcgtcct tggcgcgctt ctggccggcc acccagagct tgttcgtgtt
     4501 gacgaacagg ttgtgcggca cctcgcgccc atcgtcgcgg gtgaacggcg gtgcgggatc
     4561 gccctcggtc atgcccacca cgcccgactc cttgatgatc ttcacgaccc ggttcgcgcc
     4621 gcccgagtag gccagcagcg gatgggggct gaactgcagc aggatcttgg ggtcggtggt
     4681 acgggcgctg cgcaccgggc cgacacgctc ggcgctcgaa cagttgaaga ccacgatgaa
     4741 ccgcgtgata ccgccttcga cgacctcttc gtagacgacg tcggccttgt ccaggcccac
     4801 gagcggcatt gcgtcaggcg tgttctccac cttgatggcg agcgcggggc gggccggcgg
     4861 ttccttgtcc tcgccgaccg gctggttcgt gagcgggcac ggtgtggggg gtgtctggtc
     4921 accgggaacg acggggtcga gcaccttggc gacggggttg tcgccgttca gagcgagggc
     4981 ggcgacgcct ccgccgagca cggcaacgac cgcgcctccg gcgatcaacg ccttgccgcg
     5041 agtggtgagt gccacggtgg gggaacctcc tggggaccgg ttggatgtcg gttcgcatgg
     5101 ccctgttcga agggcccttc atgaggatgg gtcccgacat ggggacccga actgatgcta
     5161 ccggcggttc gcggacgcct caaacacccg ggcccacttc gacatgaacg tcacatctcc
     5221 tccagccggc ggatccgctc ggcgagcggg gggtgggtcg agaacagccg ctccatcgtg
     5281 gagttcttct cccccgggat gcgcgatggc tggttgagcc acagatgggc ggtggcgttg
     5341 ttggccgacc gcatcgccgt cggcgcggtt tccagcttcc gcagcgccga gatcaatccg
     5401 ggcggatacc gtgtgagcaa ggcaccctga gcatcggcga ggtactcgcg gttgcgcgag
     5461 accgacagct tgatcacctg gccggccagt ggcgcgatcg ccatcaacgc gaaccccacc
     5521 gcgaacatga tcaggccgaa cccgcccccg ttcgaatcgt tgccacggcg tccgccgatc
     5581 ccgccccacc accagctgcg catgaagaac tccgagagca gcaccgcggc gcccaccagg
     5641 gtggcgacga tggtgcccag caggatgtct cggtcgacga cgtgggacat ctcgtgggcg
     5701 atcacgccct cgagctccac gcggttcatc atggttagca ggccttcggt cacggcgatc
     5761 gacgaatgct cggggtcgcg ccccgtggcg taggcgttgg gcgcctgctc gggcaccaca
     5821 tagaccttcg gcttcggcac gcccgcggcg atcgcgatgc cctcgacgat gttgtgcagg
     5881 cgcggctgtt cgtcgggggt caccagcttg gcgcgcgtgg aggcgaggac caccttgtcg
     5941 cccgagaagt acgaccaggt ggccaggagg aacgccacca ccagggcgat cgccaggccg
     6001 agcggccccg tgccgaagag gtagccgatc gcgaggccga tcacgcccag gaagaggaag
     6061 gccccgatga cgagcaggac cgacttccgt ttgttcgacg cgatctcttc gtacatctag
     6121 gtgcccgggg tgagatccac ccgcaccggt cctcgttccg gatcgtcgat gtcgaagaag
     6181 tcgcgggtgg tgaacccgaa cgccttggcc accaggttgg aggggaacga gcccaccagc
     6241 gtgttcagct tcatcacctg gtcgttgtag aactgccggg cgtaggcgat cttcgactcc
     6301 gttccggtca gctcctcctg caacgcgagg aagttctggt tcgccttcag ctccggatag
     6361 ttctcggcga ccgcgaacaa ctggcgcaga ccgcccgtga tcgcgttctc cgcctgggcc
     6421 tgctggtcga cgccatcggc ggccatggcg cccgccctgg cctcggtgac gtgttcgaag
     6481 acctcgcgtt cgtggctcgc atagcccttc acggtctcga cgaggttggg gatcaggtcg
     6541 tatcggcggc ggagctggac gtcgatctgc gaccagccgt tgtcgacccg ggtgcggagc
     6601 gccaccaggc ggttgaacag gtagacgccc gccaggagca cgacggcgag cagcccgacc
     6661 acgatccaga gcaccggtga catgcgccta tcgtatcgac ggtggcgacc cggtggccgc
     6721 ccgctcgtag atcgcctcca gccgtgcggt gaccaccgac cagtcgtagg tcgccgctcg
     6781 tgcccgaccc gctgccgaca ggcgttcggc gagtgccgg
//
LOCUS       DATDBZ010000009         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_2550,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000009 DATDBZ010000000
VERSION     DATDBZ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VIX62_00235"
     CDS             complement(<1..186)
                     /locus_tag="VIX62_00235"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEY5858696.1"
                     /translation="MPNLRDFERRLGGLVEGLFSKTFRSGVQPIELAKRLIKEMDAGR
                     TVGVNEVWAPNHFDFSLS"
     gene            complement(232..606)
                     /locus_tag="VIX62_00240"
     CDS             complement(232..606)
                     /locus_tag="VIX62_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858697.1"
                     /translation="MTERSQHIDFDELMRVLTVTGFIALATWVTASSIGVISGYVAVS
                     PPATLRFLLAVLILVFARRTYWELREWRWGKLPPDERFGFSNPLAEHPRTSEFGFAQG
                     PGGGDEAEPTDTAATGSSHPRA"
     gene            728..814
                     /locus_tag="VIX62_00245"
     tRNA            728..814
                     /locus_tag="VIX62_00245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:762..764,aa:Leu,seq:cag)
     gene            885..1595
                     /locus_tag="VIX62_00250"
     CDS             885..1595
                     /locus_tag="VIX62_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5858698.1"
                     /translation="MSTFVVVPGAWDTPATMEPLIDPLESAGHAVIVVDLPCEDADAT
                     LERYAGAMRVALPDDLADVVLVGYSFGGFTASRIAVEHPDVPVVYVAAWIPRDGASVL
                     DLFVGGDPFEAGEEAGIAAFDGLILSAGPGLCALNIDRYVAAADPSEQDAIRSYLERT
                     QRPQGIAVLREKWRGELPTSRRRTYVLTTADTLVPPEAQRVMAASVGAEVVEIDTDHG
                     PFREQPERLVELLVATTR"
     gene            1870..2244
                     /locus_tag="VIX62_00255"
     CDS             1870..2244
                     /locus_tag="VIX62_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858699.1"
                     /translation="MGTKAETIRVSARPAGFTLWTAAVSALAAAALIMSAVALTVATR
                     DRAVTGVAGDGIHEAASTEASLWNAGKLDAMEGRMLAESVRINGYVPLWNAGKLDAME
                     GRMLAESVRINGYNMEGRGQAG"
BASE COUNT          377 a          818 c          861 g          399 t
ORIGIN      
        1 ggagagcgag aaatcgaagt ggttcggcgc ccagacctcg ttgaccccga cggtgcgccc
       61 ggcgtccatc tccttgatca gacgcttcgc gagctcgatc ggctgcacac cggatcggaa
      121 cgtcttggag aagagcccct ccacgaggcc ccccagacgt cgttcgaagt cgcggagatt
      181 gggcacaggc gtcacacggg gcctgcggga gccccgttcc cggcgcctat gttacgcccg
      241 ggggtgtgac gaaccggttg cggccgtgtc ggtcggttcg gcctcgtcgc cgccgcccgg
      301 tccctgggcg aacccgaact cggatgtccg ggggtgctcg gcgagcgggt tcgagaagcc
      361 gaaccgctcg tcgggcggca gcttgcccca ccgccactcg cgcagttccc agtaggtgcg
      421 ccgcgcgaac accaggatga ggaccgccag caggaaccgc agggtcgcgg gcggagagac
      481 ggcgacgtag ccggagatca cgccgatcga ggatgccgtc acccaggttg cgagcgcgat
      541 gaaaccggtg accgtcagta cccgcatcag ctcgtcgaag tcgatgtgct gtgatcgttc
      601 ggtcatggca gaacggggta tcggcagcta cccggttccg ggtacatcgg gcgaagggat
      661 ggttctcgcg gtcactcgtt cgggggacgt cgcgcgccga ggtcttgtta cgatgccggt
      721 cccactcggg cgagtggcgg aattggcaga cgcgcaggat tcaggttcct gtgtccgaaa
      781 ggacgtgggg gttcaactcc cccctcgccc accagccaga cttgcgcgat gacccctccc
      841 cgcgtttgat acgcccttgt acgcagagca ggaggacccg ttcgatgtcc acgttcgtcg
      901 tcgttcccgg agcctgggat acgcccgcca cgatggagcc gctgatcgat ccgctggagt
      961 cggccggtca cgcggtgatc gtcgtcgatc tgccgtgtga ggacgccgac gcgaccctcg
     1021 aacgttacgc gggggcaatg cgcgtcgcgc tccccgatga cctggccgac gtcgtgctgg
     1081 tcggctactc gttcggaggc ttcaccgcct cgaggatcgc cgtggagcac cccgacgtgc
     1141 cggtggtcta cgtcgcggcc tggatcccgc gcgatggcgc gtcggtgctc gatctgttcg
     1201 tggggggcga tccgttcgag gccggcgaag aagcggggat cgccgccttc gacgggctga
     1261 tcctcagcgc cggcccaggt ctctgcgccc tgaacatcga tcgctacgtt gccgcggccg
     1321 atccgtccga gcaggacgcg atccgctcgt acctcgagcg gacgcaacgc ccgcagggca
     1381 tcgccgtgct gcgtgagaag tggcgaggcg agctgccgac ctcccgccgc cgcacctacg
     1441 tcctgaccac cgccgacacg ctggtgccac cggaggccca gcgggtgatg gcggcatcgg
     1501 tgggcgcgga agtcgtcgag atcgatacgg atcacgggcc cttccgtgag cagcccgagc
     1561 gcctcgttga gctgttggtc gccaccaccc gctgacgttt ccgccgtcgg cgcgggcgac
     1621 gccctggtga cttgccatcg ggtaagagat cggccaggac ggtcagcggc ggtcacaggc
     1681 cctgccactt cacgcgcggc atgaccgccc ttgaccagcc gtagccgctc tatcgtcact
     1741 caggcacgcg tttcaggcga aggcgtaccg gccccacgtt cgatcggtac ggatttcggg
     1801 gattcctcgc gtcgccgctc cccctagcct cgggctcacg atcggtaccg aggtgagagg
     1861 aggagaacca tgggcacgaa ggccgaaacg atcagggtct cagcccggcc tgcggggttc
     1921 acgctctgga ccgcggccgt gtccgccttg gccgccgcag cgctgatcat gagcgcggtg
     1981 gcgctcaccg tggccacgcg cgatcgcgcg gtcaccggtg tggcgggcga cggcatccac
     2041 gaggcggcga gcaccgaagc gtcgctctgg aacgcgggca agctcgacgc catggagggc
     2101 cgcatgctgg ccgagagcgt tcggatcaac gggtacgtcc cactctggaa cgcgggcaag
     2161 ctcgacgcca tggagggccg catgctggcc gagagcgttc ggatcaacgg gtacaacatg
     2221 gaaggccgtg gtcaggccgg gtagctcgtc ggtcgaggcg gcaagttcgc gctcaggttc
     2281 gcccgggtgg ccgggcggac ctcgagcgcg cggtcgttcg gggtctgcgc agcgttcggg
     2341 ttcccaccct agaatcctcg cgtccaccga ccctgggagt gcgctgatgg ccggagacgt
     2401 catcgggcga gagtccgagc tcgcgatcct cgatcgcttc ctcgactcga tcccg
//
LOCUS       DATDBZ010000010         3549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2458030, whole genome shotgun sequence.
ACCESSION   DATDBZ010000010 DATDBZ010000000
VERSION     DATDBZ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2458030"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VIX62_00260"
     CDS             complement(<1..195)
                     /locus_tag="VIX62_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEY5858700.1"
                     /translation="MTEYLDPTDVEALLDEEGFHYKDGTRGRNLLLSALAAPMPVFGE
                     DVHPTLEEKAAALLLAVNRNH"
     gene            complement(192..428)
                     /locus_tag="VIX62_00265"
     CDS             complement(192..428)
                     /locus_tag="VIX62_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003797348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY5858701.1"
                     /translation="MASTPNSEVTTMAMTLRLDEQHDELLDALAERFGRSKTEVVEIA
                     LDELAAREDRSARTRAAFARVRERDADLLERLSR"
     gene            complement(482..1339)
                     /locus_tag="VIX62_00270"
     CDS             complement(482..1339)
                     /locus_tag="VIX62_00270"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY5858702.1"
                     /translation="MTGHVGSAAARELLDRGVPVRAVVRDPAQREEFSQQGALTAVAD
                     LGDRAALSRALRGSDGAFVLLPISPTGGDAEHRRLADSIAGAVGDSGIRHVVMLSSIG
                     ADLPDGSGPIRWVHHLEQRLSETGAVLSAIRSWHFQEKVETILPAVLGEGVYPVFGDS
                     ADVPTQMIATRDIGRAVAETLLAPPSASQVIDLEGPVYTERDVATHLEAILGRALQVV
                     TIPRPGWVGAMLGAGLPESFAHELAALYDAEQNDRFHARGDRRYTCTTGIDETLRHVV
                     EAATSRAVR"
     gene            1482..2456
                     /locus_tag="VIX62_00275"
     CDS             1482..2456
                     /locus_tag="VIX62_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY5858703.1"
                     /translation="MDPLADLLDGVRARTSAFCQAILDPPWGLRIDDRAALALATTLS
                     GHAWIVPDDGDPVLMRTGDVAIVKGPHPYTVADDPSTVPDLIVHPGNRLTTMDGVDVT
                     DAWRLGPRTAGRTRDGSALVASGTYQVTNDVSERLLNALPDIVLVPAADAPKPAMDFL
                     AYEVGNDGPAQQVLLDRLLDIALVATLRAWFARPGTAAPGWYRAHGDPLAGPALRLIH
                     EDPAHPWTVAGLAAEVGGSRAAFARRFTSLVGEPPMTYLTGWRIARAADLLRATDHTI
                     EAIAHQVGYANAFALSVAFKRIRGVSPQRYRAGEGSAALAGAVRVGAS"
     gene            complement(2553..3200)
                     /locus_tag="VIX62_00280"
     CDS             complement(2553..3200)
                     /locus_tag="VIX62_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEY5858704.1"
                     /translation="MAGRRTYDDGCGIARALDLVGERWALLVVRELLLGPKRFTDLRT
                     GLPGVSANILAQRLRELEESGVVLRRRMEPPAGSWVYELTESGRGLEQVILDLGRWGL
                     QSTSLPHDAPIGVDSVILSLRARFDPKAAADLRGEYELRLDGYRFRAVVDDGRIDLAR
                     GASEHADAVIETTPGRLDSLVSGELSVTELVGTGGATVEGDLAAVERFATLFSAS"
     gene            3305..>3549
                     /locus_tag="VIX62_00285"
     CDS             3305..>3549
                     /locus_tag="VIX62_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010063819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5858705.1"
                     /translation="MGNPISATTSEAGPAAPDPRRWLVLAVLGSAFFIVILDGTIVYV
                     AVPQIQVSLGFTEASVQWVLSAYLLTFGGLLLFGGRLA"
BASE COUNT          533 a         1264 c         1186 g          566 t
ORIGIN      
        1 atggttccga ttgaccgcga ggagcagtgc cgccgccttc tcctcgagcg tcggatgcac
       61 gtcttcgccg aacacgggca tcggcgcggc gagtgccgag agcaggaggt tccggccgcg
      121 ggtgccgtcc ttgtaatgga agccctcctc gtcgaggagg gcttccacgt cggtcgggtc
      181 gaggtactcc gtcaccggga gagacgctcg agcaggtcgg cgtcacgctc gcggacccgc
      241 gcgaacgcgg cgcgcgtgcg agccgacctg tcttcacgcg cggccagctc gtcgagggcg
      301 atctcgacga cctcggtctt cgagcgcccg aatcgctccg caagggcgtc gaggagttcg
      361 tcgtgctgct catcgagtcg caacgtcatg gccatcgtgg tcacctccga gttcggggtc
      421 gaggccattc taggggaggt ggtatcaccg cggtatcaac cgaaccccta cgtcgaagga
      481 atcacctgac ggctcggctc gtggccgcct cgacgacgtg gcgcagtgtc tcgtcgatcc
      541 ccgtggtgca ggtgtatcgc cggtcgcccc gcgcatggaa gcggtcgttc tgctcggcgt
      601 cgtagagcgc cgcgagctcg tgggcgaacg actccgggag gccggcaccg agcatggctc
      661 cgacccatcc cggtcgagga atcgtgacca cctggagggc tctcccgagg atcgcctcga
      721 ggtgggtcgc gacatcccgc tccgtgtaga ccggtccctc gaggtcgatg acctggctgg
      781 ccgatggcgg cgcgaggagc gtctcggcga ctgccctgcc gatgtcgcgc gtggcgatca
      841 tctgcgtggg gacgtcggcc gagtcgccga agaccgggta gacgccctcg ccgagaacgg
      901 ccggcaggat cgtctccacc ttctcctgga agtgccacga acggatcgca ctcagcacgg
      961 cgcccgtctc gctcaagcgc tgctcgaggt ggtgcaccca cctgatgggt ccgctgccat
     1021 cggggaggtc ggcaccgatc gacgagagca tgacgacgtg tcggatgccg ctgtcgccga
     1081 ccgcgccggc gatcgaatcc gcgaggcggc gatgctcggc gtccccgccg gtcgggctga
     1141 tgggcaggag cacgaacgct ccatcgctgc cacggagcgc acgagacagc gccgcccgat
     1201 cgccgaggtc ggcgaccgcg gtcagtgctc cctgctggct gaactcctcg cgctgggcag
     1261 ggtcgcgcac cacggcccgc accggaacac cccggtccag caactccctg gcggccgccg
     1321 aaccgacgtg gcctgtcact cctgtgatcg tgtacatgcg attccctctt cgttctctgc
     1381 gcccgctctt cgggctcgtg ggtcccaagt caatcgtcgt gtatcgagat gatccatatc
     1441 tgaaagccgc gattccatgt ctgtgcgtct acgatggggg catggatccg cttgctgacc
     1501 tcctggacgg ggttcgcgcg cgcacgagcg cgttctgcca ggccatcctc gatccgccgt
     1561 ggggcctgcg catcgacgac cgcgccgccc tcgccctggc cacgacgttg agcgggcacg
     1621 cctggatcgt gcccgacgac ggcgatcccg tgctgatgcg caccggagac gtggccatcg
     1681 tcaaggggcc gcacccctac acggtcgccg acgacccgag cacggtcccc gatctcatcg
     1741 tccatcccgg caatcgcctg accacgatgg acggcgtcga cgtcacggat gcctggcggt
     1801 tgggaccccg caccgccggt cgcacccgcg acggctccgc cctcgtggcc agcggcacct
     1861 accaggtcac caacgacgtc tccgagcgcc tcctcaacgc gctccccgac atcgtgctgg
     1921 tgcccgcagc cgacgccccg aaaccggcga tggacttcct ggcgtacgag gtcggcaacg
     1981 acggcccggc ccagcaggtc ctgctcgacc ggctcttgga catcgcgctc gtcgcgacgt
     2041 tgcgcgcgtg gttcgcccga cccggcacgg cagcaccggg ctggtaccgg gctcacggcg
     2101 acccgctggc cggcccggcg ttgcggctca tccacgagga ccccgcccat ccgtggaccg
     2161 tcgccgggct cgcagccgag gtcggcggtt ccagggccgc cttcgcgcgc cggttcacct
     2221 cgctggtcgg cgaaccaccc atgacctacc tgaccggctg gcgcatcgcg cgcgctgccg
     2281 acctgctccg tgccaccgac cacaccatcg aagccatcgc gcatcaggtc ggctatgcca
     2341 acgcgttcgc gctcagcgtc gcgttcaagc gcatccgcgg cgtatcgcct cagcggtacc
     2401 gtgccggcga gggatccgcg gctctggctg gggcggtgcg ggtcggcgca tcctgaaccc
     2461 ggccgcaacg agtcccccac gtcgggcgtg gtggaggcgc aaggccgggc ggatgccgcg
     2521 atccgcgctc agggcaacgg cgtcctcgcc gatcagctcg ccgagaagag cgtcgcgaac
     2581 cgttcgaccg ctgccaggtc gccctcgacg gtggctccgc cggtgcccac cagctcggtc
     2641 accgacagct cgcccgagac caacgaatcc agtcggcctg gcgtggtctc gatgaccgcg
     2701 tcggcatgtt cggaagcgcc gcgagccagg tcgatccggc cgtcgtcgac gaccgccctg
     2761 aaccgatacc cgtccaggcg cagctcgtac tcgccccgga ggtcggcggc agctttcggg
     2821 tcgaatcgcg ctcgcaggga gaggatcacc gagtcgacac cgatcggagc gtcatgcggc
     2881 agtgaggtcg actgcaggcc ccaacgtccg aggtcgagga tgacctgctc gaggccacgt
     2941 ccgctctccg tcaactcata gacccaggag ccggcgggcg gctccatccg gcggcgcagg
     3001 accacgcccg actcttcgag ttcacgcagt cgctgggcca ggatgttcgc gctcaccccg
     3061 gggagaccgg tccgaaggtc ggtgaatcgc ttcgggccga gcaggagctc gcgaaccacg
     3121 aggagcgccc accgctcccc gacgaggtcg agtgcgcggg cgatgccgca cccatcgtcg
     3181 tacgtgcgtc gtccggccat gcgctcagtc tacgcgggag gagcacaaag caactttcta
     3241 gtagttatta ctaacgctct agtagtaccg tcgtactcat cgagcgagga cgaggtgacg
     3301 catcatgggc aacccgatca gtgcaacgac gtccgaggcc ggcccggcgg cgccggatcc
     3361 acgaaggtgg ctcgtgctgg ccgtgctcgg cagcgccttc ttcatcgtga tcctcgacgg
     3421 cacgatcgtg tatgtcgccg ttccgcagat ccaagtgagc ttggggttca cggaagccag
     3481 cgtgcagtgg gtgctgagcg cctatctcct gacgttcggc ggcctcctgc tcttcggcgg
     3541 tcgcctcgc
//
LOCUS       DATDBZ010000011         5435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1640248, whole genome shotgun sequence.
ACCESSION   DATDBZ010000011 DATDBZ010000000
VERSION     DATDBZ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640248"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..3364
                     /locus_tag="VIX62_00290"
     CDS             221..3364
                     /locus_tag="VIX62_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858706.1"
                     /translation="MTGGTAARQRVQAAPEVSPTAAWVSQRRRLASSVMVSAFVAATT
                     VIAMAEIDRLIAGVLTAEGRSSPLVSVIGPLALIGRDAWSDWASSTAADSVGWWIIAS
                     VLLDAVFIASYVWLFSRVLRLIAVSDSRRFAKRVLFALILLEALEGVVLIVGAIALMV
                     TLNPGDLGDLPRAIVAPESPFTVVVAVLATLKCGAIVLLAILVLRDGAARDRLGRLVK
                     RLAQAVWLHRLSAVLVLTLLVFTCIPAADVLDQLPDLQRQWVPNDWGSDWPAVGIAAR
                     QSVLASVSVGLAAGAALILGRARTRALAASITALDVPPARIHALWWSAPILVWAALWV
                     VTGLTTGTWAPSWSAIAFLVVPFIVILSYLWPDAPWPITPRPDRDARARWAWLTGDAI
                     AASIAGIAGLGLVRSFTAPVFTGAALPRLESLTYGVSVALLVIGLGIAVASPFLLRAP
                     EPRAPELSAPELRASQRFVALLDPERRVDELPGAEALRRLHLIIIVGFFLAGLLVLAW
                     VGFYPVDAAAFVGAPGLTVLLLTAWGAVLGAFTVGVQEHPPAPIFRFMRLRADPVLTL
                     AITVPLVWSVIAAGLSIDDARLHATRTGPDASAGTLTDAPDEVDESAFADQLDARLGD
                     LATSGCEATAGSETFVPALIVVAEGGGIRAAYWTARAMEKLAEDECLGQSVLLSSGVS
                     GGSVGLALTAVRGRAADSELRALADPDAVGTGAAALLVGDVVASATGLRFPSVLEEGV
                     KWRDRAALIEEVWIDKVPGLAGLADLGASDRIGIPVLNSTDVRTKCKVLVSNDVATSG
                     GTDAGNAPDAAGTAESASTAPDCETTSTAPAASLPVPAQCFDDMDWATAAMLSARAPV
                     ITPAARVVDDVCGPEEMQLVDGGYAEGSGLGTAADLAPVIADRISVHNAQADGDNPPI
                     VPIVVYLKNSAGYDLRQDLESVAAEPLVPIVGVAALSKAGTADVLLQRASSALGTVGD
                     EGGAAARAVQAVDSSFPGLAVTVAPSTVPAVVPPFGWALSDLSVATLERALERQVVPP
                     DGWTVATLGDLLAATVG"
     gene            complement(3440..3826)
                     /locus_tag="VIX62_00295"
     CDS             complement(3440..3826)
                     /locus_tag="VIX62_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5858707.1"
                     /translation="MPRSTVNAPSAPAAIGPYSQAATGEGTLFLSGQTPIDPATGLLI
                     EGDVGAQTRRVFDNLNAVLQEAGGSLDDVVKVNVYLTSMSDFAAMNAVYADVFSEPFP
                     ARTTVAVAGLPLDARVEIECVAAVPR"
     gene            4504..5298
                     /locus_tag="VIX62_00300"
     CDS             4504..5298
                     /locus_tag="VIX62_00300"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5858708.1"
                     /translation="MSGTVTGAPGDTDFMLTLYPLGGPDSEVYDHMAFADPDFTAEVP
                     PGRYHVHIDTAESASAWLGGSLFAAGSEVLVVPSTGTTLDIVLSADFGSISGSVDGFP
                     AGLHQLITVEAFAYDAATNGAGVGRYTATLIGDGFDGAFTIAGLAPGTYQLRGYSDPD
                     VGGATRYLPERTVVTVSAGSTTDVGALTFTEEPIDFERVSGADRFRTAAALSAEFFDA
                     TATDTVFIASGVAFPDALSAGPAAAVSEAPVLLVPRWGSPPRWSGN"
BASE COUNT          740 a         1877 c         1897 g          921 t
ORIGIN      
        1 ctcgatcagc cccgccgact cgtagtaccg gatcgtgctc gccggcaacc cagtccgttc
       61 ctcgcagtcg cgccggtcga ccgcctggac gcggccgcgg ggtgaggagg ctcgatcgcc
      121 cgtcgacgcg caggctcgcg cgattgctgc ccccggacgg ggggagacgg ggaacgggcg
      181 cacaggacta gcatcccgat caggtcaggg agggggaccc atgaccgggg gaacagcagc
      241 gcggcagcgg gtgcaggcag cacccgaggt cagcccgacg gcggcgtggg tgagtcagcg
      301 acgccgactg gcgtcatccg tcatggtctc ggccttcgtg gccgccacga ccgtcatcgc
      361 tatggcggag atcgatcggc tcatcgcagg cgtcctcacc gccgagggac gcagctcgcc
      421 cctcgtcagc gtgatcggcc cgctcgcgtt gatcggacga gacgcgtggt ccgactgggc
      481 gtcgtcgact gccgccgact ccgtcgggtg gtggatcatc gcgtcggtgc tgctcgacgc
      541 ggtgttcatc gcgtcctacg tgtggctctt cagtagggtg ctgcgcctga tcgccgtcag
      601 cgactcgcgc cgattcgcga agcgggtcct gttcgcgttg atcctgctcg aggcgctcga
      661 gggggtcgtt ctcattgtcg gcgcgatcgc cctgatggtg acgttgaatc ccggcgatct
      721 cggtgacctc ccgcgcgcga tcgtcgcacc cgaaagcccg ttcaccgtgg tggtcgcagt
      781 cctggcgacc ctcaagtgcg gagcgatcgt gctcctcgcg atcctggtgc tcagggatgg
      841 cgccgcgcgc gatcgcctcg gccggctggt gaagcggctg gcacaggcgg tctggcttca
      901 ccgcctgtcg gcagtgctcg tactgaccct cctggtcttc acgtgcatcc ccgcggccga
      961 tgtgctcgac cagctgcccg acctgcagcg gcagtgggtc ccgaacgact ggggctccga
     1021 ctggcccgcc gtagggatcg ccgcccgcca gagtgtgctg gcttcggtgt cagtcggact
     1081 cgccgcgggc gcggccctca tcctcggccg tgctcggacg cgcgcgctcg cggcatcgat
     1141 caccgctctg gatgtcccgc cggcgcgaat acacgcgctc tggtggtcgg ctcccattct
     1201 ggtgtgggcg gccctgtggg tcgtcaccgg cctcacgacc ggcacgtggg cacccagctg
     1261 gtcggccatt gccttcctgg tcgtcccatt catcgtcatc ctgagctacc tctggccgga
     1321 cgctccgtgg cccatcaccc cgcgccccga ccgcgacgcg cgtgcgcgat gggcatggct
     1381 caccggcgac gccatcgccg cctcgatcgc aggcatcgcg ggactcggcc tcgtgcgctc
     1441 cttcacggcg cccgtcttca cgggtgcggc cctgccgcgc ctcgagagcc tcacttatgg
     1501 ggtgtccgtc gcgctcctcg tcattggcct tgggatagcg gtggcctccc cgttcctcct
     1561 ccgcgcaccc gagcctcgcg cacccgagct cagcgcaccc gagcttcgcg catcccagcg
     1621 gttcgttgcc ctgctcgacc cggagcggcg tgtcgacgaa ctgcccggag cagaggccct
     1681 gcgacggctc cacctcatca tcatcgtggg gttcttcctc gccggtctcc tcgtcctcgc
     1741 atgggtgggc ttctacccgg tggatgccgc ggcgttcgtc ggcgcaccgg gcctcacggt
     1801 gctgttgctc acggcctggg gcgccgtcct cggcgcgttc acggtcggag tccaggagca
     1861 cccgccggcc ccgatcttcc gattcatgcg cttgcgggcc gacccagtgc tcacgctcgc
     1921 catcacggtt ccgctcgtct ggtcggttat tgccgccggg ctcagtatcg acgacgcccg
     1981 gctgcatgcc acgcgcacgg ggccggatgc gtcggctgga accttgaccg atgccccgga
     2041 cgaggtcgac gaatcggcgt tcgccgatca gctcgatgcc cggctcggcg atctcgcaac
     2101 gagcggatgc gaggcgaccg cgggaagcga gacgttcgtg cccgcgctga tcgtggtcgc
     2161 cgaaggcggc ggtatccggg cggcctactg gacagctcgg gcgatggaga agctcgccga
     2221 agacgagtgc ctcgggcagt cggtgctgct ctcgagcggc gtcagcggcg ggtcggtggg
     2281 gctcgcgctc accgcggtcc gtgggcgagc cgccgattcc gagctccgcg ctctcgcgga
     2341 cccggacgcg gtcggcaccg gtgccgccgc gttgctcgtg ggggacgtcg tcgcgtcggc
     2401 gaccggcttg cggttcccgt cggtcctaga ggagggcgtc aagtggcgcg accgggccgc
     2461 cctcatcgag gaggtctgga tcgacaaagt gcccggtctc gcgggcctag ccgacctcgg
     2521 ggcatccgac cggatcggca taccggtgct caattcgacc gacgtcagga cgaagtgcaa
     2581 ggtgctcgtc tcgaacgacg tcgcaacgtc gggcggcacg gacgccggca atgcgccgga
     2641 tgcggccggc accgctgaat cggcgagcac agcgccggat tgcgagacca catcgaccgc
     2701 cccagccgcg agcctgcccg tcccggcaca atgcttcgac gacatggatt gggcgaccgc
     2761 cgcgatgctg tcggcgaggg ccccggtgat cacgcccgcg gcgagggtgg tggacgacgt
     2821 gtgtggcccg gaggagatgc aacttgtcga cggcgggtac gcggagggct cgggactcgg
     2881 caccgccgcc gacctcgcgc cggtcatcgc cgaccgcatc tcggtccaca acgcgcaggc
     2941 cgacggagac aacccgccca tcgtgccgat cgtggtgtac ctcaagaact ccgcagggta
     3001 cgacctgcgt caggatctcg agagcgtcgc cgccgagccg ctcgtgccga tcgtcggggt
     3061 cgccgcgctg tcgaaggccg gcacggccga cgtcctgctc cagcgggcgt cgagcgcgct
     3121 cgggacagtc ggcgacgagg gcggggcagc cgcgcgcgcc gtccaggcgg tggactcgtc
     3181 gttccccggg ctcgccgtga cggtcgctcc gtcgaccgtc cccgccgtgg tcccgccatt
     3241 cggttgggca ctcagcgacc tcagcgtcgc gaccctcgaa cgggcgcttg agcggcaggt
     3301 tgttccgccg gatggctgga cggtggcgac cctcggcgac ctcctggcgg cgaccgtggg
     3361 ctgactccta tatccgaccc tgggcgtcgg gccggatcag gttccgccag ctcgccggct
     3421 ccctcggatc tccggtcgac tatctcggca ccgcagctac gcactcgatc tcgacccgag
     3481 catcgagcgg aaggccagcg accgcgaccg tcgtgcgagc cgggaacggc tctgagaaca
     3541 cgtctgcgta caccgcattc atcgccgcga agtcgctcat cgaggtgagg tagacgttca
     3601 ctttcacgac gtcatccaga ctcccacccg cctcctgcaa cacggcgttg agattgtcga
     3661 acacgcggcg tgtctgagcg ccgacgtcac cttcgataag gagcccggtg gcggggtcga
     3721 tcggcgtctg ccctgagagg aatagtgtgc cctctccagt cgcggcctgg gagtacgggc
     3781 cgatcgcagc gggagccgac ggagcgttca cggttgagcg aggcatgagc tcatcgtctc
     3841 aggtcgatcc tcacgcgaac acctccgcgc tttccggatt gggaaactca ccgctggaac
     3901 cagcctgacg atccgtgtcg gcaccgcctt tcagtttcga tcgtacggtg acgtggcact
     3961 gagaccactt gggcgaccat tccgcaatgg ccgggggccg atgaggccat gtcggttagg
     4021 gggccgtggg tgcatagcgc gtaacgcgat tggagccggc gccggccgtt cgcgtcagcc
     4081 cgcgagcgct cgttcgcgcc cgaggcgttt ctcgtaggct cgcgcccgct cgcacgcgtg
     4141 taccgcacgt cggggtgcac cactcgcgtc gtgggtggtt cctcaggaag aaaagtacgc
     4201 atcccggccg ttcgcaccgg cgcagccggg gtgcatcggc accggtgaac aatacgatcg
     4261 cgtcactcgc gagggccccg agcacctggt cgattggcgc tgccgcagcc tgcgtgatcg
     4321 cgatcgcgcc catcacgtgt ggcggtgtcc aagatacagt ggggcaacgt ccgtgcggca
     4381 taaggggttg ccaaggcggg agggcacatg gtgaggtggt cagcggtcgt tgcggtcagc
     4441 gcgctggtcg ttgcgggtgc catgggcatc gcacctgcga cagctgtgga tgagcttgtc
     4501 acggtgagtg ggaccgtgac cggcgcgccg ggcgataccg acttcatgct gacgctctac
     4561 ccgctcggcg gtcccgattc cgaggtctat gaccacatgg cattcgcgga tcccgacttc
     4621 acggcagagg tcccgcctgg tcgctatcac gtgcacatcg acaccgcaga gtccgcatca
     4681 gcctggttgg gtgggtcgct cttcgcggcc gggtcggagg tgctcgtcgt tccgagcacc
     4741 ggcacgacct tggacatcgt tctatccgcc gacttcggat cgatctcggg ttcggtggat
     4801 ggtttccccg cgggcctgca ccagctcatc accgtcgagg ccttcgccta cgacgcggcg
     4861 acgaacggcg ccggggtggg ccggtacact gccacgctga taggcgacgg gttcgacggc
     4921 gcattcacga tcgccgggtt ggcgccaggg acctaccagc tgcgcgggta cagcgacccc
     4981 gatgtcggcg gcgccacgcg gtaccttccg gagcggaccg tcgtcacggt ttcggcgggt
     5041 tcgaccaccg atgtcggagc gctgacattc accgaagagc cgatcgactt cgagcgggtc
     5101 tcgggggcgg accggttccg gaccgcagcg gcgctctcgg cggagttctt cgacgccacc
     5161 gcgacggaca ccgtgttcat cgcgagcggc gtggcgttcc cggatgcgtt gagcgccggg
     5221 cctgcggcgg ccgtgagtga ggcgccggtg ctcctcgttc cccggtgggg atccccgcct
     5281 cggtggtccg ggaactgacc aggatcgcgc cctcgacgat ctacgtgatc ggaggcccgg
     5341 gcgcggtctc tgcggcatcg gaggcggcac tcggcgccta tgccgatgaa gtggtaagga
     5401 tctccgggcc cgaccggtac gcgacctccc gtgcg
//
LOCUS       DATDBZ010000012         6404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1845455, whole genome shotgun sequence.
ACCESSION   DATDBZ010000012 DATDBZ010000000
VERSION     DATDBZ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845455"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="VIX62_00305"
     CDS             <1..929
                     /locus_tag="VIX62_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5858709.1"
                     /translation="ANAGKAQLVIAHVWERPEGAEQVLQAALASAEASGVKRLVGELH
                     GGRPPADVLIDLAESKDVGLIVVSGGRSTTALGPTADRLSHHAPRDLLIVMDEVRGDA
                     DRLYRRVVIATDGSPTADRAARKGYDVAESVGAPVTLVFVGHPATGELVTRDTVAVYG
                     TQVETDVILREGDPSQEIVDVAHEVDADLIVVGNKGMQGRKRFFLNPVPEKVVDLSDR
                     DVLVARTVAQVASELQPGDGGIIVQAGERLAAFMDAQGELHVMSAKCTHMGCTVAWNA
                     GTHLFECPCHGSKFGSMGEVVNGPATRPLPPA"
     gene            complement(952..1431)
                     /locus_tag="VIX62_00310"
     CDS             complement(952..1431)
                     /locus_tag="VIX62_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858710.1"
                     /translation="MYRYWFMLHILGVVAFMAAHGASMFTMFRVRNLDLDRTRIADAI
                     SFSGTTTRPMYISLAVLVVAGFVAGVQGNWLDDWWLWIAVVVLLVTTGLMTAIAAPYF
                     RKITEACGVRPSGVPRTSDEELTALLHGPTTTLITIIGTAGLLVILYLMIFKPGQTF"
     gene            complement(1466..2317)
                     /locus_tag="VIX62_00315"
     CDS             complement(1466..2317)
                     /locus_tag="VIX62_00315"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY5858711.1"
                     /translation="MAIQDQTSALRRVFVRPPEATDAGTWHDYGWRSAPDPGRASDEH
                     EAFRAHLEAAGATLVLADTPVPGDIDAIYAYDPTLLTDAGAVLLRTGKPGRRAEPGAM
                     GLDLNAAGIEVLGTMQAPGTAEGGDMFWLDERTLLVGRGYRTNDEGIAQLGTFLEPLG
                     ADVVSFDLPHFHGESACMHLMSFISPLAPDLAVVFAPMVPVRLMEMLNDRAITLVQVP
                     DEEFDSQGPNVLALAPRVALALDGNPETRRRMESVGVDVRTYAGSEISRKGDGGPTCL
                     TRPLERG"
     gene            complement(2335..3438)
                     /locus_tag="VIX62_00320"
     CDS             complement(2335..3438)
                     /locus_tag="VIX62_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858712.1"
                     /translation="MNAHGVRIVVGEGRSSQHGLLRFVLDGEGYDVVADAANPAELAR
                     ILAAHKPDVVVLDDGIGATAVGMVREMVPGAKIVLVWPGAVVPIGGAAQVSPSRVLQE
                     LGPAVARVSGVAGVTAASAGAALVDEAKRDPDNLRRLVRGAGAASAAAAAGAIIVDGA
                     SAPLVILPVTPSVGPPAGDDVVTVPEADEIADVAAAGVGAVGVAAGVAAISTPAASAV
                     AVGAQSSLNRRLGNLALGGAAVAGAIVLALALGGAKVPIAQVSGEAFTFTPPIAPEPG
                     GGSGVPPIGGNGNHEGPGGGNHGGPGASGKLDFYPIQPYISSFLPDSSLPPVAGGPIP
                     PVEASGRRGGLNQHHRHANTNKGDHARAGNRGS"
     gene            3563..3970
                     /locus_tag="VIX62_00325"
     CDS             3563..3970
                     /locus_tag="VIX62_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5858713.1"
                     /translation="MSDQLIRWTGQGLQFEAQTTYGMSVKVGGDVEGPGAKPSDLLPV
                     SLAVCTSYDVVTILKKQRQTLVGLEVRIHAEQEREAPWAFISIHQHFVLTGVIDEAKA
                     ARAIELSEGRSCSVAATLRPVVRLSHSFEVTTS"
     gene            complement(3993..4544)
                     /locus_tag="VIX62_00330"
     CDS             complement(3993..4544)
                     /locus_tag="VIX62_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY5858714.1"
                     /translation="MTTFVDHDVTFGRRYEYRVVALGAGGPSPPSPTIVVRLPRPPTT
                     SAQLTGVYTVHLTVREAHALSSALGIEDPTPGESATDRWTFEPVCATPLSACPTQWEG
                     LGGYLVATGRSWVGTVMGPEARCGNGTKAPAPTRFDLRVNDAYGDSDAWVVGSFEGSV
                     SLSFHCPGFLVSRGSVVVRGIRR"
     gene            complement(4840..5664)
                     /locus_tag="VIX62_00335"
     CDS             complement(4840..5664)
                     /locus_tag="VIX62_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858715.1"
                     /translation="MRRSLRFSGRPLGVAVLVLCIGACSSTVVTDDRVARRVTVAPSA
                     TPAIDGLAITEPTWRSDGSNWALGLTWSVTGGTPLDHYEVRRNGEAVDESIGAATWTD
                     RDVEPGTRYRYAVIGVTADGARTRPERSVISTGTPPVADARLEGSFVMVMDATSSHGV
                     DDPVDGGRVVFRFDPTCGQGACSTIWTVRDRETDMTLARDGAIYAGRGRTPLLIRNCF
                     GDEIAEHVRARFRVVEAASRDGRWRAVEIEGAIRERSASNGCMVASIRWAVHGVIR"
     gene            5752..>6404
                     /locus_tag="VIX62_00340"
     CDS             5752..>6404
                     /locus_tag="VIX62_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858716.1"
                     /translation="MRRLPLFLIPCLAALMLVASPMPAYAQDPGSPELRTLDRKIQTA
                     ERRIRTWNERLERWHERIARSAVAVDRLTDVAEDSEPVALEGVMHGFPTRELGSHRLA
                     RAHEHLRKVLKDHTARRAQRELDAWSSVLIELRHAREHLLRREEHVEPPHVPTHTGPA
                     TYESWARAFLAKVSAPACSENLAIVVTWETSESTDAAFNPLATTHAMEGATDFNSVGV
                     "
BASE COUNT         1106 a         2115 c         2164 g         1019 t
ORIGIN      
        1 tggccaacgc cgggaaggcc caactcgtga tcgcgcatgt ctgggagcgg cccgagggcg
       61 cggaacaggt cctgcaagct gcactggcca gcgcggaggc gtcgggcgtg aagcgactgg
      121 tgggtgagtt gcacgggggt cgaccgccgg cggacgtgct catcgatctt gcggagtcca
      181 aggatgtcgg gctgatcgtc gtgagcggag gccgcagcac cacggcactc gggcctacag
      241 ccgaccggct ctcacaccac gcaccccgcg atctgctgat cgtgatggac gaggtccgcg
      301 gcgatgccga tcgcctctat cgccgggtag tgatcgccac cgatggttca ccgaccgccg
      361 accgagcggc gcggaaggga tacgacgtcg ccgaatcggt gggcgctccg gtgaccctgg
      421 tgttcgtcgg ccatccggcg accggcgagt tggtgacccg ggacaccgtc gcggtctacg
      481 gcacccaggt cgagaccgat gtgatcctgc gcgaaggcga cccttcgcag gagatcgtcg
      541 atgttgcgca cgaggtcgat gccgacctga tcgtggtcgg gaacaagggc atgcagggca
      601 ggaagcgatt cttcttgaac cccgtgccgg agaaggtcgt cgatctcagc gatcgtgacg
      661 tgctggtggc acgcaccgtg gcccaggtgg cgtccgaact ccaaccgggt gatggaggca
      721 tcatcgtgca ggctggggag cggctcgcag ccttcatgga cgcccagggt gagttgcacg
      781 tgatgtcggc aaagtgcacc cacatgggct gcacggtcgc gtggaacgcg ggtacgcacc
      841 tcttcgaatg cccgtgccat gggtcgaagt tcggatcgat gggtgaggtc gtgaacgggc
      901 cggcaacccg tcctttgcct ccagcctgac gctcgacgac ggggcgttgg ttcagaaggt
      961 ctggcccggc ttgaagatca tgaggtagag gatcacgagg agtccggcgg tcccgatgat
     1021 ggtgatcagg gtcgtcgtgg gtccgtgtag gagcgccgtg agttcttcgt cgctggtgcg
     1081 tgggacgccg gagggccgga ccccgcacgc ctcggtgatc tttcggaaat acggcgcggc
     1141 gatcgccgtc atgagtccgg tcgtgaccag caggaccacg accgcgatcc acagccacca
     1201 gtcgtccagc cagttgccct ggacgccggc gacgaatccc gcgaccacca gcaccgccag
     1261 cgagatgtac atggggcgtg tcgtggtgcc cgagaaggag atggcatcgg cgatccgcgt
     1321 gcggtccagg tcgaggttgc ggacacggaa catcgtgaac atggacgcgc cgtgggcggc
     1381 catgaacgcc acgaccccca ggatgtgcaa catgaaccag tagcggtaca tcgggcggac
     1441 tatagctgct agatacctgt gctcgtcagc cgcgttccag cggacgcgtc aggcatgtcg
     1501 gtccgccgtc gcccttgcgg gagatctcac ttcccgcata cgtgcgaacg tcgacaccga
     1561 ccgactccat gcgtcgtcgg gtctcggggt tgccatccag cgccagggca acccggggcg
     1621 cgagcgccag cacgttgggt ccctgtgaat cgaactcttc gtcgggaacc tggacgaggg
     1681 tgatcgcccg atcgttcagc atctccatca gccggacggg caccatcgga gcgaacacca
     1741 ccgcgaggtc gggagcaagt ggcgagatga atgacatcag atgcatgcat gccgactcac
     1801 catggaagtg cgggagatcg aacgacacga cgtctgcgcc gagcggctcg aggaatgtac
     1861 cgagttgcgc gatcccttcg tcgttggtcc ggtagccgcg gccaaccagg agggtgcgct
     1921 cgtcgagcca gaacatgtcg ccaccctcgg cggtcccggg ggcctgcatg gtgcccagga
     1981 cctcgatccc ggctgcgttc aggtcgagtc ccatggcccc cggctcggcg cgacgacccg
     2041 gtttgccggt gcgaagcaag acagccccgg cgtcggtgag gagggtgggg tcataggcgt
     2101 agatggcgtc gatgtcgcct gggaccggag tgtcggcgag cacgagggtg gcaccggccg
     2161 cctccaggtg cgcgcggaac gcctcgtgct catcggaggc cctaccgggg tcgggcgccg
     2221 atcgccagcc gtagtcgtgc caggtgccgg catcggtggc ctcaggtggc cgaacgaaga
     2281 cgcgccgaag ggctgaggtt tgatcctgga tggccatgcg tggatgctac gcggtcaaga
     2341 accgcggttg cccgcacgag cgtggtcgcc cttgttcgtg ttggcgtgcc ggtgatgctg
     2401 attgagacca ccacgacgcc cggacgcttc cacgggaggg atgggacctc ctgcgacggg
     2461 gggcagcgag gaatccggca ggaacgagga gatgtagggc tggatcgggt agaagtcgag
     2521 cttgccactg gcaccaggtc ctccgtggtt gccgccgcct ggtccttcgt ggtttccgtt
     2581 gcccccgatg ggagggacgc ctgatccgcc gccgggctcg ggggcgatcg gtggcgtgaa
     2641 cgtgaacgcc tcgccgctga cctgggcgat gggcaccttc gcgccgccga gcgccaatgc
     2701 caggacgatg gcgcctgcca ccgcagcccc accgagggcc aggttgccga gccgacggtt
     2761 gagcgagctc tgggcaccca cggcgacggc cgacgccgca ggggttgaga tcgcggccac
     2821 tcccgcggcg acgcccacag cgcccacacc ggccgcggcg acgtccgcga tctcgtcggc
     2881 ttcgggaacg gtgacgacgt cgtcgccggc aggcggaccg acagacggcg tgaccgggag
     2941 gatcacgagg ggtgctgatg cgccatcgac gatgatggcc ccggccgccg cggcggccga
     3001 cgccgctcca gcaccacgga ccaagcggcg gaggttgtcg gggtcgcgtt tggcctcatc
     3061 gaccagggcg gcaccggcag aggccgccgt caccccggcg acgcccgaga ccctagcgac
     3121 ggcaggtccg agttcctgca ggactcgaga cggagacacc tgcgctgctc cgccgatggg
     3181 gaccaccgcg cccggccaga ccagcacgat cttggcccca ggcaccatct cgcgaaccat
     3241 gccgacggcg gtggccccga taccgtcgtc gagcacgacg acgtcgggct tgtgagccgc
     3301 aaggatcctc gcgagttcgg ccggattggc tgcgtcggcg acgacgtcgt agccttcgcc
     3361 atcgaggacg aaccgcagga gcccatgctg ggaggagcgg ccttcaccga ccacgatccg
     3421 cacgccgtgg gcattcatgc gttcgctgtc ccccgttcga ccgtcatcga aggtgagatt
     3481 acctcagggg gcgcgcgcgt tgaggtcatc gtctgaccca aaaactatgg cccagtcgta
     3541 ctagttaccc gaaggagcgc cattgtccga tcagctgatc cggtggacag ggcaaggatt
     3601 gcagttcgaa gcgcagacca catatggcat gtcggtcaag gtcgggggcg atgtcgaggg
     3661 tccgggggcg aaaccatcgg atctgctccc ggtgtcgctt gctgtctgca cgtcgtacga
     3721 cgtcgtgacg atcctgaaga agcaacgcca gacactcgtg ggcctcgagg tccggatcca
     3781 tgcggaacaa gaacgcgagg ctccctgggc attcatctcg atccaccagc acttcgtcct
     3841 gacgggcgtg atcgatgagg cgaaggctgc ccgtgccatc gagttgtcag aagggagatc
     3901 ttgttccgtt gcggccacgt tgcggccggt cgttcgactc agtcactcgt tcgaggtcac
     3961 cacgagctga tccggtcatc gacctgcccc gctcatcgac gaataccccg cacgaccacc
     4021 gatccccgtg aaacgaggaa cccggggcag tggaacgaga gactgaccga cccctcgaac
     4081 gatcccacca cccaggcgtc cgagtccccg tacgcatcgt tgacccgcag gtcgaaccgg
     4141 gtaggggccg gcgccttggt cccgtttccg catcgtgcct cgggacccat caccgttccg
     4201 acccaggatc gcccagtcgc gaccaggtag ccgccgagtc cttcccactg ggtggggcag
     4261 gcactcagcg gcgtggcgca cacgggctcg aacgtccacc ggtcggtggc gctctcaccc
     4321 ggcgtgggat cctcgatgcc caatgccgag ctgagcgcat gagcttctcg gaccgtgaga
     4381 tggacggtgt acaccccggt cagctgcgcg ctcgtcgtcg ggggcctcgg gagccgaacg
     4441 acgatcgtcg gggagggggg cgacggtccc ccagcaccga gcgcgaccac ccggtactcg
     4501 taccgccgcc cgaacgtcac gtcgtgatcg acgaatgtgg tcatcggcgc ggtctcttca
     4561 ccgaggccga ccaacgtcac cccatcgcgc tcgatgcgat agcccgttgg ttctcccccg
     4621 ctgggtggtt cggaccaccg aagggtgatc gacggatcga gtcggcggca cgggcctgag
     4681 catcggaagg gctcagccgt cacatcgccg ggagctcccg gcggtcctgg ggcacgtgtc
     4741 agcaaccacc cggccacgat cccgcacacg gccgccgacg cgatcacgac ccagacgcgc
     4801 cctccactcc gcccgctcat cctcgctcga gaccggccac taccggatga caccgtggac
     4861 ggcccatcgg atcgaggcca ccatgcagcc gttcgacgcg gagcgttccc tgatggcacc
     4921 ttcgatctcc acggcgcgcc atcgtccatc tcgcgatgct gcttcgacca ctcggaaccg
     4981 agccctcaca tgctccgcta tctcgtcacc gaagcagttg cggatcagca gcggggtgcg
     5041 gccacggccg gcatagatcg ccccgtcgcg agccagcgtc atatcggtct cacgatcacg
     5101 gacggtccat atggtggaac acgcaccctg accacacgtg gggtcgaatc ggaacaccac
     5161 ccgtccgccg tcgaccggat cgtccacgcc gtgcgaggac gtggcatcca tgaccatcac
     5221 gaacgaacct tcgagtcggg catcagcgac ggggggtgtg ccggtgctga tcaccgaacg
     5281 ctcgggccgt gtccgtgctc catcggccgt gacgccgatc acggcatacc gataccgggt
     5341 tccgggctca acgtcgcgat ccgtccaggt tgccgccccg atcgactcat ccacggcttc
     5401 accgttgcgc cttacctcgt agtggtcgag gggcgttccg ccggtgacgc tccacgtgag
     5461 ccccaatgcc cagttcgagc cgtcggagcg ccaggttggt tcggtgatcg ccaacccgtc
     5521 gatcgccggc gtcgcggaag gagcgacggt gacacgtcga gcgacacgat cgtcggtgac
     5581 gaccgtggag ctacacgccc cgatgcacag gacgagcacc gcgacgccga ggggtcggcc
     5641 cgagaaccga agagatcgtc gcatctgcct ccgatgctag tcgcctgtgg ggcgatcctg
     5701 cgtctgacgg tcaagggatc gggcccgttc gtcgatacca tccccgtctc catgcgacgc
     5761 cttcccctct tcctcatccc gtgtctcgct gcgctgatgc tcgtggcgtc gccgatgccg
     5821 gcatatgcgc aggaccccgg ctcgcccgaa cttcgcacgc tcgatcgcaa gatccagacg
     5881 gccgaacgtc gcatccgaac ctggaacgaa cgcctcgagc gatggcacga acggatcgcc
     5941 aggtccgccg tcgcggtgga cagactcacc gatgttgcgg aggacagcga gccagtcgcg
     6001 ctcgaaggtg tgatgcatgg attcccgacc agggaactcg gttcgcatcg tctcgcacga
     6061 gcccatgagc acctgcggaa ggtgttgaag gatcacactg cgcggcgagc gcagcgcgaa
     6121 ctcgatgcat ggtcgagcgt gctgattgag ctccgacatg ctcgggagca cctgctccgc
     6181 cgtgaagagc atgtcgaacc gccgcacgta cccactcata caggcccggc gacgtacgag
     6241 tcgtgggccc gagcgttcct ggccaaggtg agcgcgcctg cgtgttcgga gaacctcgcg
     6301 atcgtcgtca cgtgggaaac ctcggagtcg accgacgccg cgttcaatcc gctcgccacg
     6361 acccatgcca tggaaggtgc caccgatttc aacagcgtgg gcgt
//
LOCUS       DATDBZ010000013         3408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1435602, whole genome shotgun sequence.
ACCESSION   DATDBZ010000013 DATDBZ010000000
VERSION     DATDBZ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435602"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VIX62_00345"
     CDS             <1..424
                     /locus_tag="VIX62_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858717.1"
                     /translation="DSGAADQALPAAPDARDLRLEVVSTDFDADSLQAEAQTSAARYA
                     DATSDAQEGAALSSPAGATKTEGAARSSEALRCLRTAFEGFPGDPVRLVEASFEGTPA
                     YLAFVLEGPGADQPADQMTVWVAARDDCSIVSLTSVRL"
     gene            503..1453
                     /gene="trxB"
                     /locus_tag="VIX62_00350"
     CDS             503..1453
                     /gene="trxB"
                     /locus_tag="VIX62_00350"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY5858718.1"
                     /translation="MSDVRNVIVIGSGPAGYTAALYAARANLEPLVLKGLEAGGQLML
                     TTEVENYPGFIDGIMGPELMEQFEKQAARFGAEILAVHVTEVDLSARPFTVKAGDQTW
                     QTETIIVATGASAKWLDIPGEQQLRGRGVSACATCDGFFFKDRELMVVGGGDSAMEEA
                     TFLTKFASKVTIVHRREEFRASKIMQDRALANPKIEVAWNATIDEIHGEDAVTGVTLR
                     DTLTGETREVATDGVFMAIGHTPNTSLFEGALDLDEGGYVLVAEPRTDTSVEGVFAAG
                     DVTDTIYKQAVTAAGQGCKAAIDAERFLEAKLHAAAASVS"
     gene            1520..1843
                     /gene="trxA"
                     /locus_tag="VIX62_00355"
     CDS             1520..1843
                     /gene="trxA"
                     /locus_tag="VIX62_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY5858719.1"
                     /translation="MSNLGTVNDQEFQTSVLDSETPVLVDFWAEWCVPCHMVSPVVEE
                     IGQEKGENLKVVKLNIDENPGATRTYGVMSIPSLILFKGGQEVARVIGAKPKDAILRD
                     LEPHL"
     gene            1955..2983
                     /locus_tag="VIX62_00360"
     CDS             1955..2983
                     /locus_tag="VIX62_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY5858720.1"
                     /translation="MRIFRLGDAGPEVGDIQERLFALGLAIDDAERGGRFGPSTDQAV
                     RGFQQDRLLRVDGIVGPDTWGQLVEAGYRLGDRTLYLHSPMFRGDDVRTLQRKLNALG
                     FDAGREDGLFGANCDRAFREFQRNVGEVPDGIAGLHALSMLERMRPQDSAPSRAMVRE
                     EEELRHMRASIEGQAVAIEAGAATDDPNDVVTHAIAGAIADELTAAGAKPVVVGSEGS
                     SMSSDRAREANEMGASMCISLHLASGLPEASGPTCSYFGSASTHSPAGMLLAQLILEE
                     LEAELGVRGRLQRLTGAMLRETRMPAVQVEPVFITNAVEAALLVDPEFGGRIGRAVAA
                     GVRRFFQG"
     gene            3120..>3408
                     /locus_tag="VIX62_00365"
     CDS             3120..>3408
                     /locus_tag="VIX62_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5858721.1"
                     /translation="MRYCLMLEGQEGVTWPEWMAAAQTAERLGFEAIYTSDHYFSVMG
                     NRERGSSDAWANLAALAAVTSTIRLGTMVSPVTFRLPAVLAKCAVTVDRISG"
BASE COUNT          563 a         1164 c         1189 g          492 t
ORIGIN      
        1 ggacagcggc gccgccgacc aggcgcttcc cgccgccccg gacgcccgag acctgcggtt
       61 ggaggtcgtt tccaccgact tcgacgccga ttcgctccaa gccgaggcgc agacctcggc
      121 ggcgcggtac gccgacgcca cctccgatgc ccaggaaggc gccgccctct cctccccggc
      181 cggagccacg aagacggagg gagccgcgcg ttcatccgag gccctgcgat gcctgcgaac
      241 cgcgttcgaa ggcttcccgg gcgaccccgt gcgcctcgtt gaggcatcgt tcgagggcac
      301 tccggcctac ctggcgttcg tgctggaagg ccccggagcc gatcagccgg ccgaccagat
      361 gacggtctgg gtggctgcgc gcgacgactg ttcgatcgtg tccctgacct ccgtcaggct
      421 ctgatccgag cggatcggag ccggcgctca ggaacaaccc gggaatggcc gggcgttctg
      481 gggctgtggg aggattcacg ccatgagcga cgtacggaac gtgatcgtga tcggatcggg
      541 ccccgccggg tacacggcgg cgctctatgc cgccagagcc aacctcgagc ccctcgtcct
      601 gaagggcctg gaggccggcg ggcagctgat gctcacgacc gaggtcgaga actaccccgg
      661 gttcatcgac ggcatcatgg gccccgagct catggagcag ttcgagaagc aggcggcccg
      721 gttcggggcc gagatcctgg cggtgcacgt caccgaggtc gacctcagcg cccgtccatt
      781 caccgtgaag gcgggcgacc agacgtggca gaccgagacg atcatcgtcg ccaccggcgc
      841 cagcgcgaag tggctcgaca tccccggcga acagcagctc cgaggacggg gcgtcagcgc
      901 ctgcgccacg tgcgacgggt tcttcttcaa ggaccgcgag ctgatggtgg tcggcggtgg
      961 cgactccgcg atggaggagg ccacgttcct cacgaagttc gcgtcgaagg tcacgatcgt
     1021 gcaccgccgc gaggagttcc gggcttcgaa gatcatgcag gaccgtgcgc tggccaaccc
     1081 caagatcgag gtcgcgtgga acgccacgat cgacgagatc cacggcgagg acgcggtcac
     1141 aggcgtcacg ttgcgcgaca ccctcaccgg ggagacccgt gaggtggcca ccgacggcgt
     1201 gttcatggcg atcggccaca cgccgaacac ctcgctgttc gaaggcgcgc tcgacctgga
     1261 cgaaggcggc tatgtcctgg tggccgagcc gcggaccgac accagcgtcg aaggcgtgtt
     1321 cgcggcgggg gacgtcaccg acacgatcta caagcaggcg gtcaccgcgg ccggccaggg
     1381 atgcaaggcg gcgatcgacg ccgaacggtt cctggaggcc aagctccacg ccgcggcggc
     1441 gagtgtctca taacgaggga atcccccggc gagactgagt gttgacagac caacgtagcc
     1501 aacaaggaga accccaacga tgtcgaacct cggaaccgtg aacgatcagg agttccagac
     1561 ctcggtcctc gactccgaga ccccagtgct cgtcgatttc tgggccgagt ggtgcgtgcc
     1621 gtgccacatg gtctcccccg tcgtggagga gatcggccag gagaagggcg agaacctgaa
     1681 ggtcgtcaag ctgaacatcg acgagaaccc gggcgccacc cgcacctacg gggtcatgag
     1741 catcccgtcg ctgatcctgt tcaagggagg ccaggaggtc gcccgcgtga tcggcgccaa
     1801 gcccaaggac gccatcctcc gcgacctcga gccacacctc taagttgcgc taccgcttcg
     1861 ctgcttgagc gcaagacccc cgtcgcaccg ctgcgctgct gcttgagccc ggatccgttt
     1921 gccgctcggc ggtcggcggc gcacactgac acgcgtgcgc atcttccgac tcggagacgc
     1981 cggacccgag gtcggcgaca tccaggaacg actcttcgcg ctcggcctcg cgatcgacga
     2041 cgccgagcgc ggcgggcgtt tcggcccctc gaccgaccag gccgtgcgcg ggttccagca
     2101 ggatcggcta ctccgtgtgg acggcatcgt ggggcccgac acctgggggc agctggtgga
     2161 agcgggctac cgcttgggcg accgcaccct gtacctgcac tccccgatgt tccgcggcga
     2221 cgatgtccgc acgttgcagc gcaagctgaa cgcgctcggg ttcgatgcgg gccgagagga
     2281 cggcctgttc ggggccaact gcgaccgggc cttccgcgag ttccagcgca acgtcggtga
     2341 ggtgcccgac gggatcgccg gcctgcacgc gctgtccatg ctcgagcgca tgcgcccaca
     2401 ggattcggcg ccgtcccgcg ccatggtgcg cgaggaagaa gagctgcggc acatgcgagc
     2461 ctcgatcgag ggacaggccg tcgcgatcga agccggggcc gcaaccgacg atccgaacga
     2521 cgtcgtgacc cacgcgatcg ccggcgcgat cgccgacgaa ctcaccgctg ccggtgcgaa
     2581 gccggtcgtg gtggggagcg aaggctcctc catgtcgtcc gatcgcgcgc gggaagccaa
     2641 cgagatgggc gcctcgatgt gcatctcact ccatctcgca tcgggattgc cggaggcctc
     2701 gggccccacc tgctcctact tcgggagcgc gtcgacccat tcccccgcgg gcatgctgct
     2761 ggcgcagctg atcctggaag aactcgaggc cgagctcggt gttcgggggc ggctgcagcg
     2821 gctcaccggc gccatgctgc gagaaacgcg catgcccgcc gtgcaggtgg agccggtgtt
     2881 catcacgaac gccgtggagg cggcgctgct ggtcgacccc gagttcggcg ggcggatcgg
     2941 ccgggcggtc gcggccggcg tgcgccggtt cttccagggc taacggcggc gacggcccaa
     3001 cagggtgagc gcgaggccca gcagcgcgcc gaccaccacc gcgtggacgg cacgttcgcg
     3061 ttccgtcggg ggcgtggact gggcgggctc ggacatcgcg gaggctatgc tacgcgccga
     3121 tgcgctactg cctgatgctc gaaggacaag agggcgtgac ctggccggag tggatggccg
     3181 ccgcccagac cgccgagcga ctcgggttcg aggcgatcta cacctccgat cactacttca
     3241 gcgtcatggg caaccgcgaa cgcgggtcct ccgacgcatg ggccaacctg gcggcgctgg
     3301 cggccgtcac gtcgacgatc cggctgggca cgatggtctc gcccgtcacg ttccggttgc
     3361 cggcggtcct cgcgaagtgc gccgtcaccg tcgatcggat cagcggcg
//
LOCUS       DATDBZ010000014         4161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2050713, whole genome shotgun sequence.
ACCESSION   DATDBZ010000014 DATDBZ010000000
VERSION     DATDBZ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050713"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VIX62_00370"
     CDS             complement(<1..1219)
                     /locus_tag="VIX62_00370"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5858722.1"
                     /translation="MTDTAPPVASRFGILRPLRIRDFRLLWTGQTVSMIGDGIYFIAL
                     TWQVYEITNDDPGALAIVGIAWTIPVVLLSLFAGVLADRFDRRRLLVVGDLLRLVAIG
                     SMGVLSILDLITMPWVVGLAVLFGIGQAIFNPSLTSIVPTLVPEDLLVQANSLAQFVR
                     PVTYTLIGPLIGGIIIELFGTGWAFVVDALTFGASAVAVTMIRTRPDPTREVPTTSAW
                     TEAKEGLAYVRKERWILIALLAATVSLLATWGPWETLVPYIVRNDLNGSSVALGIVFG
                     AGGVGSVLVALIFGRQGRLPRKAVTVLYLAWALGMLMTAGFGVVTEVWQAAAVALVAE
                     GSITVLIIVWYTLLQRLVPGELLGRVASLDWLISAAGVPLSFAIVGPLSKAIGVDATL
                     IAAGITGAVVTIAF"
     gene            complement(1269..2705)
                     /locus_tag="VIX62_00375"
     CDS             complement(1269..2705)
                     /locus_tag="VIX62_00375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5858723.1"
                     /translation="MTTPTVTRDRPVFEVPIVRRDAERLRSIAGAEAGSRYDSLVRTV
                     RATLRGHTVWQINATAEGGGVAELLRSCLGYLADDGIATRWLVIDADPAFFDITKRIH
                     NRLHGELGDGGPLGPEERAHYDEVTRTNLRSVLTLVARGDVVVVHDPQPLGLVPGLVA
                     AGATVVWTCHVGIDMPNAIARSAWDFLRDDVLAAHAVTFTRGAYAWHGLDADRVHVIP
                     PCIDDASRKNVALDPEHVESIVRSAGLLDHRPAGAASFLREDGSPDTIVEQAEVTEMR
                     PTPPDAPLVVQVSRWDALKDPIGVMRGFADDPSLGEAHLMLAGPRPASVADDPEADAV
                     LAEVLDVWRRLPTKDRSRVHIANLPTDDVEANAIIVNALQRRADVVVQKSLAEGFGLT
                     VTEAMWKERPIVASAVGGIRDQIRDGVHGLLVHPRDLTAFGNAVHRLLADPRRAAQLG
                     TAARQRVETHSLPTHYLGAYLALFDQLR"
     gene            2837..3241
                     /locus_tag="VIX62_00380"
     CDS             2837..3241
                     /locus_tag="VIX62_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858724.1"
                     /translation="MKLTWRDGVATVLMLGTVAVALAVTQGWNLTLLGSTKAGVAAVA
                     VIGYMMCRLGMHWITDAKDMVRGPALIAASVLGVAALVLIVAGLIVGTEALLVALTID
                     IVALWFITTWRHAVVGVPEQPNEHELQAWIHG"
     gene            3363..3908
                     /locus_tag="VIX62_00385"
     CDS             3363..3908
                     /locus_tag="VIX62_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858725.1"
                     /translation="MRRVMMVMLSAVMLLATVAGPADAVSRLQKTTGPLPMVQGYVIE
                     GACPFPVIWTDRGGRTLTTWERDHTIVSQRITGTSKVVLTNGASGLELTFDIKETTTI
                     VYDQQRGTATTLQGGSSGLVFDPGTISGAGSFTWYGGAALTTGRLDPKTLLYTDVESQ
                     HTVGLEGDVCDMLVSGLKTRH"
     gene            3950..>4161
                     /locus_tag="VIX62_00390"
     CDS             3950..>4161
                     /locus_tag="VIX62_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858726.1"
                     /translation="MPLIPGPCAGNTFDESILILGVWLLVSGGFLVAGRGRHRPSFTV
                     VGLASAAGLIGTITGLLAGFSEQGTFL"
BASE COUNT          698 a         1403 c         1431 g          629 t
ORIGIN      
        1 tgaacgcgat cgtgacgacc gccccggtga tcccagcagc gatcagggtg gcgtcaacgc
       61 cgatcgcctt cgacagaggg ccgacgatcg cgaaggacaa gggaacgcct gcggcggaga
      121 tcaaccagtc caggctggcg acccggccga ggagctcacc gggcaccagc cgctgcagca
      181 gcgtgtacca gacgatgatc aacacggtga tcgacccttc ggctaccagc gccaccgccg
      241 cggcctgcca cacctcggtc accacgccga acccggcggt catcagcatg cccagcgccc
      301 acgccaggta gaggaccgtc accgccttgc gcggaagcct gccctgccgt ccgaagatca
      361 gggccaccaa gaccgatccg accccgcccg ccccgaacac gatgccgagg gccaccgacg
      421 atccgttcag gtcgtttcgc acgatgtacg gcacgagcgt ctcccacggc ccccacgtgg
      481 cgagcaggct gacggtggcg gcgagcaacg cgataaggat ccaccgctcc ttgcggacat
      541 aggcgagtcc ctccttcgcc tcggtccacg ccgacgtggt gggcacctcg cgcgtggggt
      601 caggccgcgt gcggatcatc gtgacggcga ccgccgaggc accgaaggtc aaggcatcga
      661 ccacgaaggc ccatccggtc ccgaacaact cgatgatgat gccgccgatc aaggggccga
      721 tcagcgtgta cgtgacgggg cgtacgaatt gcgcgagcga gttcgcctgc accagcaggt
      781 cctcggggac gagcgtgggc acgatcgagg tcagcgacgg attgaagatc gcctgaccga
      841 tgccgaacag cacggccaac cccactaccc acggcatcgt gatcaggtcc aggatggaga
      901 ggacccccat cgaaccgatc gccaccagtc gcaggaggtc gccgaccacc agcagccggc
      961 ggcgatcgaa gcggtctgcg agcacgcccg cgaacaagct cagcagcacc acggggatcg
     1021 tccaggcgat gcccacgatc gcgagcgcgc ccggatcgtc gttcgtgatc tcgtagacct
     1081 gccaggtgag cgcgatgaag tagatgccgt cgccgatcat cgagaccgtc tgcccggtcc
     1141 acagcagtcg gaaatcgcgg atccggagcg ggcgcaggat gccgaaccgc gaagcgacag
     1201 ggggcgcggt gtcggtcacg ttcccaggat acggagcacc ggcgacctcg gcgttgccgg
     1261 gaaatggctc agcgcagctg gtcgaacagg gcgagatagg cgccgaggta gtgcgtgggc
     1321 aaggagtggg tctcgacgcg ttgacgggcg gccgtcccta gctgggccgc ccggcggggg
     1381 tcggcgagca gtctgtgcac cgcgttcccg aacgcggtga ggtcgcgcgg gtgaacgagg
     1441 agcccgtgca cgccgtcacg gatctgatca cggatgccgc ccaccgccga tgcgacgatg
     1501 ggacgctctt tccacatcgc ctcggtcacg gtgagcccga agccctcggc caggctcttc
     1561 tgcaccacga cgtcggctcg gcgctgcagc gcgttgacga tgatcgcgtt ggcctccaca
     1621 tcgtcggtgg gcaggttggc gatgtggacc ctgctccgat ccttcgtcgg caagcgcctc
     1681 cagacgtcga ggacctcggc gagcaccgca tccgcctcgg gatcgtcggc caccgatgcc
     1741 ggccggggtc ccgccagcat caggtgggcc tcgccgaggg acgggtcgtc cgcgaacccg
     1801 cgcatgacgc cgatcgggtc cttcagggca tcccatcgcg acacctgaac cacgagcggg
     1861 gcgtcgggag gcgtcgggcg catctcggta acctcggcct gctccacgat cgtgtcgggc
     1921 gagccgtctt cgcggaggaa cgacgccgca ccagctggac gatggtccag caagcccgcc
     1981 gaccggacga tcgactcgac atgctctgga tcgagcgcga cgttcttgcg ggacgcatcg
     2041 tcgatgcatg gggggatcac gtggacgcgg tcggcgtcga gcccatgcca ggcgtaggcg
     2101 ccccgggtga aggtgacggc atgcgcggcg aggacatcgt cccgcaggaa gtcccaggcc
     2161 gaccgcgcga tcgcgttggg catgtcgatc cccacgtggc aggtccacac cacggtcgca
     2221 ccggcggcaa ccaggccggg taccagcccg agcggctgag gatcgtgcac gaccacgaca
     2281 tcgccgcgag ccaccagggt gaggaccgag cgcaggttcg tccgcgtgac ctcgtcgtaa
     2341 tgcgcgcgtt cctccggccc caggggtcct ccgtcgccga gttccccgtg gagccggttg
     2401 tggatccgct tcgtgatgtc gaagaacgcg ggatcggcgt cgatgacgag ccagcgggtg
     2461 gcgatgccgt catcggccag gtagccgaga cacgaccgga ggagttccgc gacgccgcca
     2521 ccttcggccg tcgcgttgat ctgccagacg gtgtggccgc gcagggtcgc gcggacggtt
     2581 cgaacgagcg agtcatagcg cgaacccgcc tccgcccccg cgatcgagcg gaggcgttcc
     2641 gcgtcccgac gcacgatcgg cacctcgaac acgggacggt cccgggtgac agttggggtc
     2701 gtcacgcccg ggaccgtacc gcgggttcgc gtccacgtcg cctctagcga aggtcctttg
     2761 cgtcgaggtc gcacggccgt tttcggggtc ggtgatcggt gccacggtga tggtggagtc
     2821 tcagaaggag gccgtcatga aactcacgtg gagagacggt gtcgcgacgg tgctcatgct
     2881 gggcacggtc gccgtggccc tagcggtgac gcagggttgg aacttgacgt tgttgggaag
     2941 caccaaggcc ggggtcgctg cggtcgccgt gatcggctac atgatgtgcc gcctgggcat
     3001 gcactggatc acggacgcca aggacatggt gcgtggtccc gccttgatcg ccgcatcggt
     3061 gctcggggtg gccgcgctgg tcctgatcgt tgccggtctg atcgtgggca ccgaggcgct
     3121 gctggtcgca ctgaccatcg acatcgtggc gctgtggttc atcacgacct ggaggcacgc
     3181 cgtggtcggc gttcccgagc aaccgaacga gcacgagctg caggcctgga tccacggctg
     3241 atcgccacac gatctccgcc acatggccct tggccaaggc cgggtctgtg tgggaccatc
     3301 cttggacggc ttgcgacccc cctggcggtg atcgcctccg caggcccagg aggtggcgag
     3361 cgatgcgtcg cgtgatgatg gtgatgctct cggcggtcat gttgctcgcc acggtggccg
     3421 gacccgctga cgcggtctcc cggctgcaga agaccacggg cccactcccg atggtgcagg
     3481 gctacgtgat cgagggcgcg tgcccgttcc ccgtgatctg gaccgatcgc ggcggccgca
     3541 cgctcaccac ctgggaacgc gaccacacga tcgtctcgca gcggatcacc ggcacctcca
     3601 aggtggtcct gacgaacggc gcctccgggt tggagctcac gttcgacatc aaggagacga
     3661 ccacgatcgt ctacgaccag cagcgcggaa cagcgacgac gctgcagggc ggatcgtcgg
     3721 ggctcgtctt cgacccgggc accatctccg gcgcgggctc gttcacctgg tacggcggcg
     3781 cggcgttgac caccggccgg ctcgacccca agacgctgct ctacaccgac gtcgagtcgc
     3841 agcacaccgt cggactcgag ggcgacgtct gcgacatgtt ggtctcaggc ctgaagaccc
     3901 gccactgagg agttcactcc ggcaacatgg gctcggctag gcttcaaccg tgccactgat
     3961 cccaggtccc tgcgccggga acacgttcga cgaatcgatc ctgatccttg gggtgtggct
     4021 cctcgtctcg ggcggattcc tcgtcgccgg tcgaggtcgt catcgaccgt cgttcaccgt
     4081 cgtcgggctg gcgtccgccg ccggactcat cggcacgatc accggcctgc tcgcggggtt
     4141 cagcgagcag ggaacgttcc t
//
LOCUS       DATDBZ010000015         2117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1232110, whole genome shotgun sequence.
ACCESSION   DATDBZ010000015 DATDBZ010000000
VERSION     DATDBZ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232110"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1288
                     /locus_tag="VIX62_00395"
     CDS             44..1288
                     /locus_tag="VIX62_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5858727.1"
                     /translation="MLGNTLAASFIWGINTLFLLDAGLSNMEAFAANAFFTAGMLIFE
                     IPTGVVADTVGRRASYLIGTVTLAGSTALYWMLWVWESPFWAWAIVSVLLGLGFTFFS
                     GAVDAWLVDALKATGYHGSLETVFGRAQIVGGIAMLAGSVLGGVIAQLTDLGVPFLIR
                     AGILAVMFVVAALLMHDVGFTPERGESPLQATRTVFRASVKYGLGNPPVRWLMLASPF
                     TAGVGIYVFYALQPYLLELWGDEEAYTVAGLAAALLSGASILGGALAPWVRKLFRHRT
                     SSILLAAVSSAIVLLGLGLVRNFWVAIALVAVWGIASAIDDPVHRAYLNDMIPSKQRA
                     TVLSFDSLMGSGGGVVFQPVLGRVADVGGYGASMIWSGVISAIAIPFILLSRAQHSPA
                     DTAHEVNEDADAEADAGKPSSD"
     gene            1352..>2117
                     /locus_tag="VIX62_00400"
     CDS             1352..>2117
                     /locus_tag="VIX62_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005272818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY5858728.1"
                     /translation="MSSSALVVGPMLRYTAETCATVWVETDADAVLTVRAGEREWSSR
                     TFAVHGHHYALIALDDLAPGSVLPYEVRVNGERVWPESGSPMPDPVIATREPEKRLRM
                     AWGSCRTSVDHDAKGNRTHGVDAMRAFALALSEDESLRPDLILLLGDQVYADMTTEAM
                     QEFIRDRRDIKEPPGKELKDYEEYAHLYRLAWSDPANRWLLSTLPSLMIFDDHDVRDD
                     WNASRDWKRQMEATSWWHGRIVAGLSSYWVYQHLGNL"
BASE COUNT          299 a          726 c          726 g          366 t
ORIGIN      
        1 gttgccgagt ccgcgacggg tggtgggcac ctactacacg ctgatgctgg gcaacacgct
       61 cgcggcatct ttcatctggg gcatcaacac gctgttcctg ctcgacgccg ggctgtccaa
      121 tatggaggcc ttcgccgcca acgccttctt caccgccggg atgctgatct tcgagatccc
      181 gacgggcgtg gtggccgaca ccgtggggcg gcgtgcgtcg tacctgatcg gcaccgtgac
      241 gctcgccgga tcgaccgcgc tgtactggat gctctgggtg tgggagtcgc cgttctgggc
      301 gtgggcgatc gtgtctgtac tcctcgggct ggggttcacg ttcttctccg gcgcggtcga
      361 cgcctggctc gtcgatgccc tgaaggcgac cggctatcac ggcagcctcg agaccgtctt
      421 cggccgcgcg cagatcgtgg gcggcatcgc gatgctcgcc ggctccgtgc tcggcggcgt
      481 gatcgcccag ctcaccgatc tcggcgttcc cttcctgatc cgcgccggaa tcctcgccgt
      541 gatgttcgtc gtcgccgcgc tgctgatgca cgacgtcggc ttcactcccg agcggggaga
      601 gagcccgctc caggcgacgc gcaccgtgtt ccgcgcgtcg gtgaagtacg ggctgggcaa
      661 cccgccggtg cgctggctga tgctcgcgag cccgttcacc gccggggtgg gcatctacgt
      721 cttctacgcg ctgcagccgt acctgctcga actgtggggc gacgaagagg cgtacacggt
      781 ggccggtctg gcggctgcgc tgctctcggg tgcatcgatc ctgggcggag cgctcgcccc
      841 ctgggtgcgc aagctgttcc ggcatcgcac ctcgtcgatc ctgctcgccg cagtctcgag
      901 cgcgatcgtg ctgctcggtc tcgggctcgt ccgcaacttc tgggtcgcca tcgccctcgt
      961 ggccgtgtgg ggcatcgcct ccgcgatcga cgaccccgtg caccgggcct acctcaacga
     1021 catgatcccg tcgaagcagc gcgccaccgt gctgtcgttc gactcgctga tgggctcggg
     1081 cggcggcgtg gtcttccagc ccgtcctcgg ccgtgtcgcc gacgtgggcg gctacggcgc
     1141 gtcgatgatc tggagcggcg tgatctcggc gatcgcgatc ccgttcatcc tcctgagccg
     1201 cgcccagcat tcccccgccg acaccgcgca cgaagtgaac gaggacgccg acgcggaggc
     1261 ggatgccggg aagccatcgt ccgactgagc acgccgaggc ccgcacggca accggtggca
     1321 cgcacgggcc aggggcgcta cacatggacc catgtcatcc tctgcgctcg tggtggggcc
     1381 gatgctgcgc tacaccgccg agacctgcgc gaccgtctgg gtcgagaccg acgccgacgc
     1441 cgtgttgacc gtgcgcgcgg gcgagcgcga gtggagctcg cggaccttcg cggtgcacgg
     1501 ccaccactac gcgctcatcg cgctcgatga cctcgccccc ggatcggtgc tgccgtacga
     1561 ggtgcgggtg aacggggagc gggtgtggcc cgaatccggc tcgcccatgc cggacccggt
     1621 catcgcgact cgagagcccg aaaagcgact gcgcatggcg tggggatcgt gtcgcacgag
     1681 cgtggatcac gacgcgaagg gcaaccgcac gcacggggtc gatgcgatgc gcgcgttcgc
     1741 cctggctctg agcgaggacg agtccctgcg gcccgatctc atcctcttgc tgggtgacca
     1801 ggtctacgcc gacatgacca ccgaggcgat gcaggagttc atccgcgatc ggcgcgacat
     1861 caaggagccg ccgggcaaag agctcaagga ctacgaggag tacgcgcacc tctaccggct
     1921 cgcctggagc gacccggcga accgctggct gctctcgacg ctgccgagcc tgatgatctt
     1981 cgacgaccat gacgtgcgcg atgactggaa cgcctcacgg gactggaagc ggcagatgga
     2041 ggccacctcg tggtggcacg gccgcatcgt cgccgggctg agttcgtact gggtgtatca
     2101 acacctcggc aacctct
//
LOCUS       DATDBZ010000016         6261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_618950, whole genome shotgun sequence.
ACCESSION   DATDBZ010000016 DATDBZ010000000
VERSION     DATDBZ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618950"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VIX62_00405"
     CDS             <1..1171
                     /locus_tag="VIX62_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY5858729.1"
                     /translation="PTEVEFVGPVRPRLALTEISLDAQVQEAFSPPGRAAAAAELGEA
                     LAAGWRRYFAWEIAFVTLGAVVVLGAIAGWRGAGWKRTLVIVLGGLVFVQAVNLTAIM
                     VTAFTAPDILRGVGSVNEMVGRDELRPIPAATGPTQPDVQAVVLGDSTAAGLGGPPLA
                     DAAPDDTACQRSSIAFAETLARVNEWNVDNLACSGATIDDGILGLQQVGDRRIAPQLA
                     VAKRASEAEVVIVNVGANDMRWSSLVFLCAASASCDNRALTALFQRSLDDFTQDYYEM
                     LRQLSAIPGDPLVLINRYYAPFDPTLDCLEPLGLTSEKLVVLLDRLDALNDVLTAGAE
                     AFGHRTVQPDFAGHELCTDQSYVQGPDDPAPLHPNARGHLVIALADERALLDEAA"
     gene            complement(1191..1457)
                     /locus_tag="VIX62_00410"
     CDS             complement(1191..1457)
                     /locus_tag="VIX62_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY5858730.1"
                     /translation="MDDIGFFDIVWYIIAGLIIGGLARLLLPGKQSMGWIATMILGVV
                     GAIIGGLIWNAIFSGNEGVAWIGSIIAAIILLVIYERVVAKKQA"
     gene            1610..2755
                     /locus_tag="VIX62_00415"
     CDS             1610..2755
                     /locus_tag="VIX62_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY5858731.1"
                     /translation="MTLPEHTRLNWGPWYRRSPFFEATLRAGCSAYDVYQHMYHPNTY
                     GDPTEEYWALVNDVTLWDVSVERIVEITGPDSSAFTNRLTCRDLTTCAVKQGKYMLVT
                     AEDGGIVNDPVLLRVEENRWWLALADSDAGLYAMGVAVNSGLDVKVTHPEVYPVQVQG
                     PKAKDTMRDLFGDWILDMKYYWCDEADLDGIPVVISRTGWTAVVGYEIYLRDPSRGDD
                     LWQRILDAGRPHGIRVTPSSDIRRIEAGIFDWGSDIGLTDNPFEVTGLERLVQAQEAD
                     YIGKDALERIRREGVSRKLIGIELEGEPVDQPTQHWPVAHGGETVGHVTDACWSPRLE
                     KNIGYVWVPIALATPGSRLDVETDDGIRAGTTAVIPFIDPQKKVPAA"
     gene            2880..3092
                     /locus_tag="VIX62_00420"
     CDS             2880..3092
                     /locus_tag="VIX62_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY5858732.1"
                     /translation="MTESIYRVTEVIGTSTASWEDAAKNAVETAAESLRDLRVGEVVK
                     LDLTIEDGKVAHYRARVNISFKYEHE"
     gene            3300..4505
                     /locus_tag="VIX62_00425"
     CDS             3300..4505
                     /locus_tag="VIX62_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858733.1"
                     /translation="MKKALTVLLSFGLLLILTASSAHALPGTDANAVAEPWPEVGPLS
                     GSRVRAVQVVGGVIWLGGKFSNATDSTGATRSVSNLVAFDAGTGRFASVSAPGFTSSG
                     AEIWDLDVRGGELIVGGKFVNPANSAQKNLAAVNLSTGAVRWFKGVPTAKTVLVNGDS
                     VYAAGTTLGLWNVASGSPIWKGRTKVTVQAGLRGHSTPPAYRELVLAGGALFAACQCD
                     FAGGQPVKALIKLSADGVLDPSWVPSDLTPGGASGASAATGIAVATDGSALYLGAGGS
                     DFFAKYSFAEALQWKRDTSGSVQSLEIMGGQVVVGGHFVEIADATGDRCGFKSNNPGD
                     LDPFGECVRHNYLAAYGTNGALDAWTPSVTGKFNGIWDLEAEGAALHFGGEFTKVNGV
                     NATFYGKLT"
     gene            complement(4533..4667)
                     /locus_tag="VIX62_00430"
     CDS             complement(4533..4667)
                     /locus_tag="VIX62_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858734.1"
                     /translation="MASKDKGGAKGSKTAASKTLKEKRAAKKAKGKGGSSSSDWNLNK
                     "
     gene            complement(4756..5424)
                     /locus_tag="VIX62_00435"
     CDS             complement(4756..5424)
                     /locus_tag="VIX62_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein"
                     /protein_id="HEY5858735.1"
                     /translation="MKDAPLLRGSLLLRLVALFVGLLLIAVAIVAMLESELGVPPWDV
                     LHMGIAEHTPLTLGTAAVVVGLVIVAIGWIGGAPPGFGTIANAVVIGLAIDVFSAIGW
                     VEQLSDASLGVRVGLVALGVWLFGVGSALYIGAGMGAGPRDSFMLALTRRTGWRIGIV
                     RGSIEVTVLVVGLLLGGLAGIGTLALALLVGPSVELSFWLLLRLDLADPGLHAPEFGP
                     LDVA"
     gene            complement(5513..5656)
                     /locus_tag="VIX62_00440"
     CDS             complement(5513..5656)
                     /locus_tag="VIX62_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858736.1"
                     /translation="MTPLLALGDGTIFAIGSVIFIAVFTAALGLAYARFEELGEDDRR
                     SGG"
     gene            complement(5653..5844)
                     /locus_tag="VIX62_00445"
     CDS             complement(5653..5844)
                     /locus_tag="VIX62_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858737.1"
                     /translation="MKLGTKAAILIILGAAFVSFLLSVSLWFGVFGDPDRELGLFVGL
                     WVPSILALGALLSPRGAAR"
BASE COUNT         1072 a         2167 c         2104 g          918 t
ORIGIN      
        1 gccgaccgag gtggagttcg tggggccggt gcgaccccgc ctggccctca ccgagatcag
       61 cctcgacgcc caggtgcagg aggcgttctc gccgccgggg cgcgccgcgg cggccgcaga
      121 gctcggcgag gctctggccg cgggatggcg acggtacttc gcgtgggaga tcgcattcgt
      181 cacgctcggc gccgtggtgg tgctcggcgc catcgccgga tggcgcggtg ccggctggaa
      241 gcggaccctg gtgatcgtgc tcggcggact ggtgtttgtc caggcggtga acctcaccgc
      301 gatcatggtc accgcgttca ccgcgccaga catcctgcgc ggggtgggca gcgtgaacga
      361 gatggtgggc cgggacgagt tgcgacccat ccccgccgcc actggtccga cgcaacccga
      421 cgtgcaggcg gtcgtgctcg gagattcgac ggccgccggg ctcggcggtc cgcccctcgc
      481 cgacgcggcg cccgacgaca ccgcatgcca acgcagctcg atcgcgttcg cggagacgct
      541 cgcccgggtc aacgaatgga acgtcgacaa cctggcctgc agtggggcga cgatcgacga
      601 cggcatcctc gggttgcagc aggtgggcga tcggcggatc gcgccgcaac tggccgtcgc
      661 gaagcgcgcg agcgaggccg aggtggtgat cgtcaacgtc ggcgcgaacg acatgcggtg
      721 gagcagcctc gtgttcctgt gcgcagcgtc ggcatcgtgc gacaaccgcg ccctgacggc
      781 gttgttccag cggtcgctcg acgacttcac ccaggattac tacgagatgt tgcgccagct
      841 atcggccatc cccggcgatc cgctcgtgct gatcaaccgg tactacgcgc cgttcgatcc
      901 gaccctcgat tgcctcgagc ccctcggatt gacgagcgag aagctcgtcg tgctgctcga
      961 tcgactcgac gccttgaacg acgtgttgac ggcgggggcc gaggcgttcg gacaccgcac
     1021 ggtgcagccc gacttcgccg gccacgagct gtgcacggat cagagctacg tgcagggacc
     1081 cgacgacccg gcgccgctgc atcccaacgc gcgcggccat ctcgtcatcg cgctcgccga
     1141 cgaacgcgcc ctgctcgacg aggctgcgtg accccaatcc tcccgcggca tcaggcctgc
     1201 ttcttcgcga ccacgcgttc gtagatcacg agcaggatga tcgcggcgat gatcgatccg
     1261 atccaggcga cgccttcgtt gccggagaag atcgcgttcc agatgagccc gccgatgatc
     1321 gcgccgacga cgccgaggat catggtggcg atccatccca tcgactgctt gccgggcagg
     1381 agcagccgcg ccaatccacc gatgatcagt cccgcgatga tgtaccagac gatgtcgaag
     1441 aacccgatgt catccatggt gagccgacgc tccttccggt gatgctcgct cgtcagcgag
     1501 cccttgcggc gcatcgtagg gcggcggttg cgagaagcgc aaacgacacc gtcggatgca
     1561 tagactcgcg cccacggttc cgcgaagccg acgagtgggg ggacacgaca tgacgctgcc
     1621 ggagcacacc aggttgaact ggggaccgtg gtaccgccga tcgccgttct tcgaggcgac
     1681 cctgcgcgcc gggtgctccg cctacgacgt ctatcagcac atgtaccacc cgaacaccta
     1741 cggcgatccc accgaggagt actgggccct cgtgaacgac gtgacgctgt gggacgtgag
     1801 cgtggagcgg atcgtcgaga tcaccggacc ggattcgagc gcgttcacga accggctgac
     1861 ctgccgtgat ctcacgacgt gcgccgtgaa gcagggcaag tacatgctcg tcaccgccga
     1921 ggacggcggc atcgtgaacg atccggtgct gctgcgggtc gaggagaacc gttggtggct
     1981 cgcgctcgcc gactccgacg ccggcctcta cgccatgggc gtcgcggtga actccggcct
     2041 cgacgtgaag gtcacgcatc ccgaggtcta cccggtgcag gtgcaggggc cgaaggccaa
     2101 ggacacgatg cgcgacctgt tcggcgactg gatcctcgac atgaagtact actggtgcga
     2161 cgaggccgat ctcgacggca tcccggtcgt gatcagccgc acgggctgga cggcggtggt
     2221 cggttacgag atctacctgc gcgacccgag ccggggcgac gatctgtggc agcgcatcct
     2281 cgacgccggg aggccgcacg gcatccgcgt gacgccctcg tcggacatcc gacgcatcga
     2341 ggccggcatc ttcgactggg gctcagacat cggcctcacc gacaacccgt tcgaggtcac
     2401 cgggctggaa cgtctcgtcc aggcgcagga ggccgattac atcggcaagg acgcgctcga
     2461 gcgcatccgg cgcgagggcg tctcgcgcaa gctcatcggc atcgagctcg agggcgagcc
     2521 ggtcgaccag cccacccagc actggccggt ggcgcacggg ggcgagacgg tcggacacgt
     2581 caccgacgca tgctggtcgc cgcggctgga gaagaacatc ggatacgtgt gggtgccgat
     2641 cgcactggcg acaccgggca gccggctcga cgtggagacc gacgacggca tccgcgccgg
     2701 cacgaccgcc gtgatcccgt tcatcgatcc ccagaagaag gtgcccgcgg cgtagcgagc
     2761 ccgactcggg gcgaccggag tgcaccaccg cgacccggtc ctcgcgggac ctgtggaccg
     2821 ggtcgcgccc gggcacgcga cagacgatga tggtgggaca cggccctgga gggatgacga
     2881 tgaccgagag catctaccga gtcaccgagg tgatcggcac cagcacggcg tcatgggagg
     2941 acgccgcgaa gaacgcggtc gagaccgcgg cggaatccct gcgcgacctg cgggtcggcg
     3001 aggtcgtgaa gctcgatctc acgatcgagg acggcaaggt cgcgcactac cgcgcgcggg
     3061 tcaacatctc gttcaagtac gaacacgagt gaacgcggcc tgagcggttc cgcccgaagc
     3121 ggttgccctg caaggcacca agggaggcac cggctcggcg acatgcgtct cttgaccagg
     3181 ccttcctcat ttcggcctag gtcgaacggt tgacgctgcg gttccccctc cgcgatactc
     3241 ggggcgaccc cgacaccact cgaggcgccc tcgccccgtg aaccccccgg aggctgttga
     3301 tgaagaaggc actcaccgtc ctgctgtcgt tcggcttgct cctgatcctg accgcgtcga
     3361 gcgcccacgc cctgcccggc accgatgcca acgcggtcgc agagccttgg cccgaggtcg
     3421 ggccgctctc aggcagcagg gtgcgcgcgg tgcaggtcgt cgggggagtg atctggctgg
     3481 gcggcaagtt ctcgaatgcg accgactcga caggtgccac tcggtcggtc tcgaacctcg
     3541 tcgcgttcga tgcagggacc gggcgattcg cctcggtgag cgcccccggc ttcaccagct
     3601 cgggcgccga gatctgggac ctcgacgtac gcggcggcga gctgatcgtc ggcgggaagt
     3661 tcgtgaaccc ggccaacagc gcccagaaga acctcgccgc cgtgaacctc tcgaccggag
     3721 ccgtccgctg gttcaagggg gtgcccaccg ccaagacggt gctcgtgaac ggcgacagcg
     3781 tctatgccgc cggcaccacg ctcggcctgt ggaacgtcgc gagtggttcc ccgatatgga
     3841 aggggcgcac gaaggtcacg gtccaagccg ggcttcgtgg gcactcgacg cctcccgcct
     3901 accgcgaact cgtgctggcc ggcggggcgc tgttcgcggc ttgccagtgc gacttcgccg
     3961 gcggtcagcc ggtcaaggca ttgatcaagc tgagtgccga cggggtgctc gatccgtcgt
     4021 gggtaccgag cgacctcacg ccggggggtg cctcaggagc cagcgcggcc accggcatcg
     4081 ccgtcgcgac cgatgggagc gccctctacc tcggcgccgg gggttccgac ttcttcgcga
     4141 agtattcgtt cgccgaggcc ctccaatgga agcgcgatac ctccggatcg gtgcagtccc
     4201 tcgagatcat gggcggccag gtcgtggtgg gaggacactt cgtcgagatc gccgacgcca
     4261 ccggcgatcg atgcgggttc aagtccaaca accccggcga cctcgacccg ttcggggaat
     4321 gcgtgcggca caactacctc gccgcctacg gcacgaacgg agcgttggac gcctggaccc
     4381 cgtcggtcac cggcaagttc aacggcatct gggacctcga ggccgagggc gccgctctgc
     4441 acttcggagg cgagttcacg aaggtgaacg gcgtcaacgc gacgttctac ggcaagctga
     4501 cctgaaccga aacaggagcg ggttgagtcc cgctacttgt tcaggttcca gtccgaggag
     4561 ctcgagccgc ccttgccctt cgccttcttg gcggcacgct tctccttgag ggttttgctc
     4621 gccgccgtct tcgagccctt cgcgccgccc ttgtccttac tggccatgac tgccccttcc
     4681 gtgggcgatg tgtgggtcca cgatgtcaca catcggcgtt ccggtcgacg gacactcgag
     4741 tcggtcctcc ggggctcagg ccacatcgag tggaccgaac tccggggcgt gcaggccagg
     4801 atcggccagg tcgagtcgca gcagcaacca gaacgagagc tcgaccgagg gtccgacgag
     4861 cagtgcgagc gccagagtgc cgatgcccgc gaggccgccg agcagcagtc cgacgacgag
     4921 caccgtcacc tcgatcgagc cgcgcacgat gccgatgcgc caaccggtgc gcctcgtgag
     4981 agcaagcatg aaggagtcac gcgggccggc acccatgccc gctccgatgt acagggccga
     5041 accgaccccg aagagccaga cgccgagcgc cacgagcccg acccgaacgc cgagtgaggc
     5101 gtccgagagc tgctcgaccc acccgatcgc gctgaacacg tcgatcgcga gcccgatcac
     5161 cacggcgttc gcgatcgtcc cgaagcccgg gggtgcgccg ccgatccagc cgatcgccac
     5221 gatgacgagt cccaccacga cggccgccgt tcccagcgtc aacggcgtgt gctccgcgat
     5281 gcccatgtgc agcacgtccc acggcggcac cccgagctct gactccagca tcgcgacgat
     5341 ggccacggcg atcaacagca atccgacgaa cagcgccacg agccgcagca gcagtgaacc
     5401 tcggagcagg ggcgcatcct tcacccatgc acggtaacga cgggctcacc cccgcgaccg
     5461 acggcggcgg tcggggcggc cggcgggccg gcctatcggc tcacggatcg cctcacccgc
     5521 cagagcgccg atcgtcctcc ccgagctcct cgaagcgcgc gtacgccagc cccaacgccg
     5581 cggtgaacac ggcgatgaag atcacgcttc cgatcgcgaa gatcgtgccg tcgccgagag
     5641 cgagcagcgg ggtcaccgtg ccgctcccct cgggctgagg agggcgccga gcgcgaggat
     5701 cgaggggacc cacaggccca cgaagagccc cagctcgcga tcgggatcgc cgaacacccc
     5761 gaaccacagc gagaccgaga gcaggaacga tacgaacgcg gcgccgagga tgatcaggat
     5821 cgctgccttg gttccgagct tcatcatgtt ctccttccgt cgaacgcgat tcggaaccca
     5881 tgcgccgcta gacgcgatcc gggcacgtcc tgcataggtt caggagcggt gacggccgag
     5941 ccccctcggc ttcgggacct cggtgtgcgt tccgcttccg gcgagcgagg cgtgcatcgc
     6001 gtatccttgg tgaagaccgc gccgatctcg tacgagacgc gcgcgcgacg aagtctcgcg
     6061 atccttcgca cgcaccgccc tcctgggcgt tcctgcgcag aggcgcctgc cctcgacgcg
     6121 caccgaacga cgaccaggag aggacacccg aaccgtgccc accaccaccg caacagctcg
     6181 atccatcgac cgcgagcccg accagcccac gttcagggcg ctcggcgtct ccgaacccgt
     6241 ctcgcaggcc ctacggatgc g
//
LOCUS       DATDBZ010000017         3249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2870089, whole genome shotgun sequence.
ACCESSION   DATDBZ010000017 DATDBZ010000000
VERSION     DATDBZ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2870089"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /gene="groEL"
                     /locus_tag="VIX62_00450"
     CDS             complement(<1..703)
                     /gene="groEL"
                     /locus_tag="VIX62_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5858738.1"
                     /translation="MAAKIITFDEEARRSLERGMDQLANAVKITLGPKGRNVVLEKKW
                     GAPTITNDGVSIAKEIELDDPSEKIGAELVKEVAKKTDDVAGDGTTTATVLAQAIVKE
                     GLRTVAAGANPMSLKRGIERATELAVEAIKNQSKEVDDKAQIANVGAISAADPQIGET
                     IAEAVDKVGKDGVITVEESNTFGIEMDLVEGMRFDKGYISPYFVTDPERMEAVLEDPY
                     ILIANSKVSAVKDLLP"
     gene            complement(844..1221)
                     /locus_tag="VIX62_00455"
     CDS             complement(844..1221)
                     /locus_tag="VIX62_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858739.1"
                     /translation="MPPPIIGPQGRALGRRSRPIDDDVEGPPPDDDWRYRRDGAPRLL
                     ALLGVVAGFLALIVPGVFALRSYRRWQDEIILQPSFAWVMGILALWAAIVVPLLVFTD
                     FIGLAIPLAVVGPFASIVIALKS"
     gene            1334..3130
                     /locus_tag="VIX62_00460"
     CDS             1334..3130
                     /locus_tag="VIX62_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5858740.1"
                     /translation="MSIEPAWERRFTATSVGFPAWSDAVPDHLALITTRSGASQVWTY
                     DLGDGSWRQVTDDPVGIEHVRVAPDGRLVWWLDATGNERGHWVSAPFDGGEPRPLVRG
                     VPDAWSMGISMVGDRVAVGCATDDDYRVYIADGDQEARLVYRHEHPAGVGAEWPEGVG
                     GLSQDGTLLCIRHGEDGDILRSALRVLDAENGQPVGDLVDRARRLEAVAWSPRGHLLA
                     FVSEMGPFERPGLWDLDSGERRDLTLPTLPGAAWPVAWYADASALLVRHEHEGRSQLV
                     RVNVDDETTDVIADPGGEIDDGAIRPDGQVWFETSSGIDPPRIVDLGGLPVLTIEDEP
                     PPPGTAMHGFWFDNPHGEPIHAFLATPEGDGPFPTVMSVHGGPEWHEGDRFDAEVLAF
                     VDAGYAVALVNYRGSTGYGVGFREALIGNPCLPESEDVNACLDRLIADGVADPARVYW
                     SGWSWGGCLACLNAGLHPDRWRAMFAGIPAGDFVAAHWASAPALQAWDDAVYGGSPVE
                     VPEMYRERDPMTYAARVRAPVLIIAGENDSRCPVEGITPWVEAVRANGVEVRVHWYPT
                     GHHANATEDQVRHMRMILDFFAEHGGPPTPTA"
BASE COUNT          489 a         1070 c         1146 g          544 t
ORIGIN      
        1 ccggcagcag gtccttcacg gccgagacct tcgagttggc gatcaggatg tacgggtcct
       61 cgagcacggc ttccatgcgc tcggggtcgg tgacgaagta gggcgagatg tagcccttgt
      121 cgaaccgcat gccctcgacg aggtccatct cgatgccgaa ggtgttcgac tcctcgaccg
      181 tgatgacgcc gtccttgccg accttgtcga cggcctcagc aatcgtctcc ccgatctgcg
      241 ggtcggcggc ggagatggcg ccgacgttgg cgatctgggc cttgtcgtcg acctccttgg
      301 actggttctt gatggcctcc acggcgagct cggtggcgcg ttcgatgcca cgcttgaggc
      361 tcatcgggtt ggcgccggcc gcgacggtgc gcaggccttc cttcacgatc gcctgggcga
      421 gcacggtggc cgtcgtcgtg ccgtcgccgg cgacgtcgtc ggtcttcttg gcgacctcct
      481 tgaccagctc ggcgccgatc ttctcggagg ggtcgtcgag ctcgatctcc ttggcgatcg
      541 agacgccgtc gttcgtgatc gtgggggcgc cccacttctt ctcgaggacc acgttgcggc
      601 ccttggggcc gagcgtgatc ttgacggcgt tggcgagctg gtccatgccc cgctcgagcg
      661 aacgccgcgc ttcctcgtcg aaggtgatga tcttcgcagc catcccttct cctttccttc
      721 gcgttggtgg cttgttcagc cctggtcagt cttggtctcc gactgctagc actcgatggt
      781 accgagtgct aacgattgta acgggaaagg gcgtgggtgt gacaagccct ctttcggacg
      841 cgatcaggac ttcagcgcga tcacgatgct ggcgaacggg cccactacgg ccagcgggat
      901 cgccagaccg atgaagtcgg tgaatactag gagcggcacc acgatcgccg cccagagcgc
      961 gaggatcccc atcacccagg cgaaggacgg ctgcaggatg atctcgtctt gccaccggcg
     1021 atacgaacgc agggcgaaga cgccggggac gatcagcgcc aggaacccgg caacgacccc
     1081 caggagcgcg aggagcctgg gggcgccatc acgtcggtag cgccagtcat cgtcgggcgg
     1141 cgggccttcc acgtcgtcat cgatcggtcg cgagcgacga cccaaggccc ggccctgggg
     1201 accgatgatg gggggaggca tgctcccgga gcccgacatc gtccgatggt gaccgctgga
     1261 tcgccgaacg gcaaggcatc ggcgtcatcc cggcgatagg ctcgcgactg tccgcatcga
     1321 ggaggaacgc gagatgtcga tcgagcccgc ctgggaacga cggttcaccg ccaccagcgt
     1381 cggattcccc gcgtggtccg atgctgtgcc cgaccatctg gcgctgatca cgactcggag
     1441 cggcgcgtca caggtgtgga cgtacgacct cggcgatggc tcgtggcggc aggtcaccga
     1501 cgacccggtg gggatcgagc acgtgagggt cgcccccgac gggcgactgg tgtggtggct
     1561 cgatgcgacc ggcaacgaac gcggtcactg ggtgagcgct cccttcgacg gcggcgaacc
     1621 gcgaccgctg gtgcgtggtg ttcccgacgc ctggtccatg ggcatctcga tggtgggtga
     1681 ccgcgtcgcg gtgggatgcg cgaccgacga cgactaccgg gtgtacatcg cggacggcga
     1741 ccaggaagcg cggctggtct accggcacga gcacccagcc ggcgtcggtg ctgaatggcc
     1801 cgaaggggtc ggaggtctct cgcaggacgg gaccctgctc tgcatccggc atggagagga
     1861 cggcgacatc ctgcgttcgg cgctccgcgt cctcgacgcc gagaacgggc agccggtcgg
     1921 cgacctggtc gatcgggccc gacggctgga ggcggtggcg tggtcaccgc gcggccacct
     1981 gctcgcgttc gtcagcgaga tgggaccgtt cgaacggccc gggctctggg acctggattc
     2041 cggggagcgc cgcgatctga cgctgcccac gctgccgggg gccgcctggc cggtggcctg
     2101 gtatgccgac gcgtcggcgc tcttggtgcg tcacgaacac gaggggcggt cacagctggt
     2161 ccgcgtgaac gtcgacgacg agaccaccga cgtgatcgct gatcccggcg gcgagatcga
     2221 cgacggcgcg atccggccgg acggacaggt gtggttcgaa acgagcagtg ggatcgaccc
     2281 acccaggatc gtcgacctcg ggggtctgcc cgtcctcacg atcgaggacg aaccgccacc
     2341 gcccgggacg gcgatgcacg gattctggtt cgacaacccg cacggcgaac ccatccatgc
     2401 gttcctggcg acgcccgagg gcgatggtcc cttccccacg gtgatgtcgg tacacggcgg
     2461 ccccgagtgg cacgagggag accgtttcga cgccgaggtc ctggccttcg tggatgcggg
     2521 ctacgcggtg gcgttggtca actaccgggg ttccaccggc tacggcgtcg gcttccgtga
     2581 agccctgatc ggcaacccgt gcctgccgga gtccgaggac gtcaacgcct gcctggatcg
     2641 gttgatcgcc gacggcgtcg ccgatcccgc acgcgtctac tggagcggct ggtcctgggg
     2701 cggctgcctg gcgtgcctca acgccgggtt gcacccggat cggtggcgcg cgatgttcgc
     2761 cgggatcccg gcgggggact tcgtggcggc ccattgggcg agcgccccgg cgctccaggc
     2821 gtgggatgac gccgtctacg gcgggagccc cgtcgaggtg ccggagatgt accgggaacg
     2881 cgaccccatg acgtacgccg cccgcgtccg tgcgccggtc ttgatcatcg cgggcgagaa
     2941 cgactcgcga tgccccgtgg aggggatcac gccgtgggtg gaggcggtgc gagccaacgg
     3001 cgtcgaggtt cgggtccact ggtatcccac cggccatcac gccaacgcca ccgaggatca
     3061 ggtgcggcac atgcggatga tcctggactt cttcgccgag cacggcggcc ccccgacccc
     3121 gacagcttga atcagtcggg cgcccaccgc cagctgctca tgaccttgtc gacgcgcacc
     3181 tcgatgatga ccgagctgcc ctcctcgatc ggagcccgct cctcgtactg cggatacttc
     3241 tcgtagagc
//
LOCUS       DATDBZ010000018         3115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_209725, whole genome shotgun sequence.
ACCESSION   DATDBZ010000018 DATDBZ010000000
VERSION     DATDBZ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209725"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VIX62_00465"
     CDS             <1..1130
                     /locus_tag="VIX62_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY5858741.1"
                     /translation="AAELFDVPAQNLVYADVDGNIGYQTPGKLPICGAGDGWMPQPGW
                     DSTYAWQGFIPFDELPYSLNPPDGYIVTANNAVVSADYPYLLTRDWDYGWRAARVLDL
                     LQRKSAMGKLTADDMRDIQADNEFVMGKELAAVYMDVSTGRPGPDAALDQLRSWDAQN
                     TAESGAAAYANVLWDQLVTDLFVTGREHPAPVTGQGRLFLVVEQLLETPDSAWWTNAE
                     LGVDGQAEMLEHAADEAYDRLVGLQGDNPSRWNWGSLHALSLTSATFGLSGIAPIEML
                     FNRGPYPVGGGSSVANATGWDIGADFETVTVPSMRMVVDLADFDASRWNHLTGTSGHT
                     YHPNYVDQTETWQRAELTPWPFTEAAVDAATTHRLTLVPAD"
     gene            complement(1250..1687)
                     /gene="mnhG"
                     /locus_tag="VIX62_00470"
     CDS             complement(1250..1687)
                     /gene="mnhG"
                     /locus_tag="VIX62_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEY5858742.1"
                     /translation="MTMLLRAASAVSAVSVGDSPVADAATPLDAWLNGAALVLILLGA
                     LLRLTAAIGLLRFRDVPTRLHAATKPQVLGLILICLAIALSLRSWPVVAFLVPVVLIQ
                     LATAPLSAHMVGRQAYRNGTLEEQSLFVDELAESRQTPPAAGG"
     gene            complement(1684..1953)
                     /locus_tag="VIX62_00475"
     CDS             complement(1684..1953)
                     /locus_tag="VIX62_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HEY5858743.1"
                     /translation="MSALLLVIYVVFGVAALLTLWRIVVGPSILDRAVASDVLLTEVL
                     CVLGAEMVINQHTRTLPVLLIIAAVGVFGTISIARFVARRDNVDR"
     gene            complement(1950..2633)
                     /locus_tag="VIX62_00480"
     CDS             complement(1950..2633)
                     /locus_tag="VIX62_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEY5858744.1"
                     /translation="MTAPIHHTSRPRHSAGHNAGEPARDAADRASDGTIGGRRGVLQQ
                     VWRQLPFFVWLVALWMLLWGQFTLVAFVTGVIAAVAVTRVFRLPPVELSGRVNLWYGL
                     IFVLTFFAALVRGSLLVAWQVLDFRRQPGSSIIAVHLRTDDDLIMTHTAVTASLIPGS
                     IIIEADRDRRILYLHVIGATSEEEVERQRRAILGWEARIVRAVGSRAQLEIIRRAEID
                     TPQQGGAPA"
     gene            complement(2630..>3115)
                     /locus_tag="VIX62_00485"
     CDS             complement(2630..>3115)
                     /locus_tag="VIX62_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5858745.1"
                     /translation="ILRVKGLMRAAPVIAVLYFIPAINLGGLPPFSGFIGKYALFNAG
                     AEVGTPIMIALIVGGIITSLLTLYALMRAWNLSFWREEDDSAETEERISYLGSAPAAA
                     IETERRVIPRIMTAATAGMVAVTVSLTLFAGPLYEVCARIGASLLEPVSISQLEDEAG
                     R"
BASE COUNT          541 a         1055 c         1077 g          442 t
ORIGIN      
        1 cagcagccga gctgttcgac gtgccggctc agaacctcgt ctatgccgac gtagacggca
       61 acatcggcta ccagaccccc ggcaaactcc cgatctgcgg ggcgggcgac ggctggatgc
      121 cgcagccggg ctgggattcg acctacgcgt ggcagggctt catccccttc gacgagctgc
      181 cgtactccct caatcctccc gacggctaca tcgtcacggc caacaacgcc gtcgtcagcg
      241 ccgactatcc ctacctcctc acccgggact gggactacgg atggcgtgcc gcccgcgttc
      301 tggatctgct gcagcggaag tccgcgatgg gcaagctcac ggccgacgac atgcgcgaca
      361 tccaggccga caacgagttc gtgatgggca aggagctggc agcggtctat atggacgtct
      421 ccaccggccg ccccggaccg gatgccgctc tcgaccagct gcgctcgtgg gacgcgcaga
      481 acactgcgga gtccggtgcc gcggcgtatg cgaacgtgct gtgggaccag ctcgtgacgg
      541 atcttttcgt gaccggccgc gagcacccgg caccggtcac aggtcaaggc cgtctgttcc
      601 tcgtggtcga gcagctgctg gagacccccg actccgcgtg gtggacgaac gcggagctcg
      661 gcgtggacgg tcaggccgag atgctcgagc atgccgccga cgaggcgtac gaccgactcg
      721 tcgggctgca gggcgacaac ccgtcgcggt ggaactgggg gtcgctgcat gcgctgtcgc
      781 tcaccagcgc cacgttcggc ctgtcgggca tcgcgccgat cgagatgctc ttcaaccgcg
      841 gaccgtatcc ggtgggcggc ggctcatcgg tcgcgaacgc gacggggtgg gacatcggag
      901 cggacttcga gacggtcacc gttccctcga tgcgcatggt ggtcgacctc gccgacttcg
      961 acgcgtcgcg gtggaatcac ctcaccggca cgagcggtca cacgtaccac cccaactacg
     1021 tcgaccagac cgagacgtgg cagcgcgcag agctcacacc gtggccgttc accgaggccg
     1081 cggtcgacgc cgccacgact caccggctga ccctggttcc cgccgactga tcgccccacc
     1141 tcaccgcgag actgcacccg caggtcgaga ctgcgggtat caccctcagt ctcgacgggc
     1201 agttgcagtc tcggcgtggg cggtgggtgc gtgggtgcgg gcagtcgcct cagccgccgg
     1261 cggcgggagg cgtctgccgg gactcggcga gctcgtcgac gaacagcgac tgctcctcga
     1321 gggttccgtt gcggtaggcc tggcggccga ccatgtgcgc cgacaacgga gcggtggcca
     1381 gctggatcag caccaccgga acgaggaacg cgaccaccgg ccaggatcgc agcgacagcg
     1441 cgatcgcgag gcagatgaga atcagcccga gcacctgcgg cttggtcgcc gcgtgcaggc
     1501 gcgtgggcac gtcgcggaac cgcagcaggc cgatcgccgc ggtcaggcgc agcagcgcac
     1561 ccagcaggat cagcacgagc gcggcgccgt tcagccaggc gtcgagcggg gtggcggcat
     1621 ccgccacggg actgtcgccc accgagacgg cggacactgc ggatgcggcg cgcagaagca
     1681 tcgtcatcgg tccacgttgt cccttctcgc gacgaagcgg gcgatcgaga tcgtgccgaa
     1741 gacgcccacc gcggcgatga tgagcagcac gggcagagtg cgcgtgtgct ggttgatcac
     1801 catctccgca ccgagcacgc acagaacctc ggtgaggagc acgtcggatg ccaccgcccg
     1861 gtcgaggatc gaggggccga cgacgatgcg ccacagggtg agcagcgcgg ctacgccgaa
     1921 gacgacgtag atgacgagaa gaagtgcgct catgcgggtg cacctccctg ctgcggcgtg
     1981 tcgatctcgg ctctgcggat gatctcgagc tgggcacgag atccgacggc gcgaacgatg
     2041 cgggcctccc agcccaggat cgcgcgccgc tgtcgttcga cctcctcctc cgaggtcgcg
     2101 ccgatcacgt gcagatacag gatccgccga tcccgatccg cctcgatgat gatcgaccct
     2161 ggtatgagtg aggccgtcac ggcggtgtgc gtcatgatca ggtcgtcgtc ggtccgcaga
     2221 tgcaccgcga tgatggagct gcccggctga cgccggaagt cgagcacctg ccaggcgacc
     2281 aggagcgaac cgcgcacgag cgccgcgaag aacgtgagga cgaagatcag tccgtaccac
     2341 aggttcacgc ggccggagag ctcgacgggg ggcaatcgga acacacgcgt caccgccacg
     2401 gcagcgatca cgccggtgac gaacgccacg agtgtgaact gaccccacag cagcatccac
     2461 agcgcgacga gccagacgaa gaacggcagc tgccgccaca cctgctggag caccccgcgc
     2521 ctgcccccga tcgtcccgtc tgaggcccgg tcggccgcat cgcgcgcggg ctcgccggcg
     2581 ttatgaccgg cgctgtgccg ggggcgcgag gtgtggtgga tcggcgcggt catcggcccg
     2641 cctcatcttc gagctgagag atgctgaccg gttcgagcag gctcgcgccg atccgcgcgc
     2701 acacctcgta gagcggtcct gcgaagagcg tgagcgacac cgtgaccgcg accatgccgg
     2761 ccgtcgccgc ggtcatgatg cgggggatga cgcgccgctc ggtctcgatc gcggccgccg
     2821 gcgcggagcc gaggtaggag atccgctcct cggtctcggc ggagtcgtcc tcctcccgcc
     2881 agaacgagag gttccacgcg cgcatgagcg catacagggt gagcagcgac gtgatgatgc
     2941 cgccgacgat gagcgcgatc atgatcggcg ttccgacttc ggcccccgcg ttgaagaggg
     3001 cgtacttccc gatgaagccc gagaacggtg ggagcccgcc gaggttgatg gccgggatga
     3061 agtacaggac cgcgatcacc ggtgcagcgc gcatgagtcc cttgacccgc aggat
//
LOCUS       DATDBZ010000019         6133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1232948, whole genome shotgun sequence.
ACCESSION   DATDBZ010000019 DATDBZ010000000
VERSION     DATDBZ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232948"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1544
                     /locus_tag="VIX62_00490"
     CDS             246..1544
                     /locus_tag="VIX62_00490"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 4"
                     /protein_id="HEY5858746.1"
                     /translation="MGPRVVVIGGGSAYMPGIAFAFANASAAFPDAVLVLHDLDRDAL
                     HLQEHLTGAIMRSRGASGIRVEAHLDREPALEGADVVLAAFRPGGFEARHLDEKIAID
                     HGVIGQETAGPGGFAMALRSVPVVLAIADQLRRVGNDGAVLLNYTNPVQIVSEAVARF
                     ASGVPYVGLCDQTEGERRFLGRLMGCDPRSIELDTCGTNHMTFTRGVSIDGQDRTVSV
                     WARLDTLALDELETEAERRIVRLFRMFGRIPSEYMQYFLFHDEVLAEQRARGRTRAEE
                     IMELLPGVLTSYRAEASSDHPEPSMARASEEHGDFAVSVMAAMLGGERARFVLNLPND
                     GQVEDLPRMAIVETPAWVHGRVVEPVPQGALPPEVSGMVRQVAAHAELTAEAAVTGNR
                     ALAVEALALHPLIRSVGTAESLVDAYLHAHADLLPQFPVT"
     gene            1541..2833
                     /locus_tag="VIX62_00495"
     CDS             1541..2833
                     /locus_tag="VIX62_00495"
                     /inference="COORDINATES: protein motif:HMM:NF025397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858747.1"
                     /translation="MNPFEAHPPLALDDVLASVVDGRTKGMPAHEVALGDVPARGLSL
                     ADLSFPVMSLRSSALEHNVALTADYCRRAGVSLAPHGKTTMSPQLWDRQLRAGAWGLT
                     AASAQQAQVMRGVGVRRVMIANELVDPGSIRWAAACLEDPDLELWSWVDSPDAVSLLE
                     AGMRGSGVGGAHRVLIELGHPGGRTGARSVADAIEVAASVAASPVLELRGVAGYEGTV
                     CAERRPECLDAVRRYLGDLHALSVAVLLEGFIDERHEMLVTAGGSTFFDLAAEALTQL
                     RPEHAGGVRVVIRPGCTLTHDHGFYAERSPFAAVDDPAGRFHPALELWAAVSSLPEPG
                     LAIVGFGRRDTSFDQGLPVPLSIQGGDGTRRDARGITVDRVVDQHAFCRVDEGADLAV
                     GEVIRFGVSHPCTAFDKWRVMPVLDDDDVVVEAVATYF"
     gene            complement(2839..3753)
                     /locus_tag="VIX62_00500"
     CDS             complement(2839..3753)
                     /locus_tag="VIX62_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY5858748.1"
                     /translation="MTTPYDVIALGETMLSLVAIDEPLASATRFHVTHGGAETNTLVG
                     LSRLGLRTAWVSRLGTDVVGERIHRALDDEGIDLRWTRRDDHRPTGVMIRDCHGCVEY
                     RRAGSAASILSADDLEEVRVAEARAVMVTGITGLLGAEPQQAALALLKRATGLRAVDP
                     HLRPGLWGSDRGRELVLPLIANCDLALGGEHELRHLVGEDDVTVRGEALARRILELGP
                     AEVVVKRGSDGAGALDRAGRWHEHSPAPVPDVDPVGAGDAFNAGYLAARLSGADATDA
                     LVQGAHCGAAVAGVIGDTEGFPRPGERP"
     gene            complement(3750..4364)
                     /locus_tag="VIX62_00505"
                     /pseudo
     CDS             complement(3750..4364)
                     /locus_tag="VIX62_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
     gene            4504..5718
                     /locus_tag="VIX62_00510"
     CDS             4504..5718
                     /locus_tag="VIX62_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY5858749.1"
                     /translation="MSWEGSRTSLVQTSVAGTPSLLRVINERTVLESVRRVGPVSRAQ
                     IARETALSKPTVSQALTGLLRAKLVREAGRSSGGKGPTAVLYELNPHAGWVVGIDVGR
                     HWVRAAIADVTGEFVARRDERARVKSARTLITQIGAVAHGLAGDAGIRWKQVTFATVG
                     SPGVLEADRGQVAFAYALPGWGRQGLVEAVQQELGTKIAFENDVNLAALGEQWHGIGK
                     GVDNFVYLHLGTGVGMGLVLNGELYRGSGGAAGEVGYLPLAGTDLRDPSSRRRGPLDA
                     AGSAAGVVATARRLGMTPPLTAKKVFTEARKGDAKAKRVVRTEAARIALAIAAISSVL
                     DPELVILGGGIGGNGDLLLEPVELELRELSPFRPRIEVSTLGEEATLHGSVSMALQAA
                     HDHLFERGKVSA"
     gene            5807..>6133
                     /locus_tag="VIX62_00515"
     CDS             5807..>6133
                     /locus_tag="VIX62_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5858750.1"
                     /translation="MKRWLVALVTFALVSAACTAGGGDDEPSINADASASHEPVTIEM
                     WSAWTSKAELEDFDKIFASFEEAYPWITVKSVGNVDDTKMLAAINSGTPPDAALSFSF
                     DNVGKFC"
BASE COUNT          877 a         2120 c         2197 g          939 t
ORIGIN      
        1 cggcgaacgc ctcctcgaac gctccgccgt ggaccccggg ctcggggaac ccgccccact
       61 ccccgctgcg caccacatcg gtgacggctg cgacgacgtc gtcatcgacg accggccacg
      121 ccgggtagtc ggtcgagcgc acgggggtgc ctccgttgat cgcgagttca gccatggtgg
      181 tgctcgctcc ttccaggtac ggacgtggtc ggccgctgcg gtgcggtgct gttagcgtgc
      241 gcggcatggg accgcgggtc gtcgtcatcg gtggtggttc cgcctacatg ccggggatcg
      301 cgttcgcctt cgcgaacgcg tccgcggcct tcccggacgc tgtgctcgtg ctgcacgatc
      361 tcgatcgcga tgccctccac ctgcaggagc acctcacggg cgccatcatg cgctcccggg
      421 gcgcatccgg catccgtgtc gaggcgcatc tcgaccgcga acccgcattg gaaggcgccg
      481 acgtggtcct ggccgccttc cggccgggcg ggttcgaagc ccggcacctc gacgagaaga
      541 tcgcgatcga ccacggcgtg atcggtcagg aaaccgcagg cccgggaggg ttcgcgatgg
      601 cgctgcggtc ggtgcccgtg gtgctggcga tcgccgacca actccgacgc gtgggcaacg
      661 acggtgccgt gctgttgaac tacacgaacc ccgtgcagat cgtgagcgag gcggtggcgc
      721 gattcgcttc gggcgtgccc tacgtcggat tgtgcgatca gaccgaaggg gaacgacggt
      781 tcctcggacg cctcatgggg tgcgacccgc gttcgatcga actcgacacc tgcggcacga
      841 accacatgac gttcacccgc ggggtctcga tcgacgggca ggaccgaacc gtgtcggtgt
      901 gggctcggct cgacaccctc gccctcgacg agctcgagac cgaggccgaa cgccggatcg
      961 tgcgcctgtt ccggatgttc ggccgcatcc ccagcgagta catgcagtac ttcctgttcc
     1021 acgacgaggt gctcgcggag cagcgggccc gggggcgcac ccgcgccgaa gagatcatgg
     1081 aactcctgcc cggcgtgctc accagctacc gagccgaggc atcgagcgac catcccgagc
     1141 cgtcgatggc ccgcgccagc gaggaacacg gcgacttcgc cgtctcggtc atggccgcga
     1201 tgttgggcgg cgagcgcgcg cggttcgtct tgaacctgcc caacgacggc caggtcgagg
     1261 atctgcctcg gatggccatc gtggagacgc ccgcctgggt gcacggtcgc gtcgtggagc
     1321 cggtcccgca aggtgcgttg cctcccgagg tgagcggcat ggtccgccag gtggccgccc
     1381 acgccgagct caccgccgaa gcggccgtca ccggcaaccg cgcgctcgcg gtcgaagcgc
     1441 tggcgctgca cccgttgatc cgttccgtgg ggacggccga gtcgctcgtc gatgcctacc
     1501 tgcatgccca tgccgatctc ctgccacagt tcccggtgac gtgaacccgt tcgaagccca
     1561 cccgccgttg gccctggacg acgtgctcgc gagcgtggtc gacggccgca ccaaaggcat
     1621 gccggcgcac gaggtcgccc tcggcgacgt ccccgcacgg gggctgtccc tcgcggatct
     1681 gtcgttcccc gtgatgtcgc tgcgctcgtc cgccctcgag cacaacgtcg ccctgacggc
     1741 cgactactgc cgccgtgccg gtgtgtcgct ggcaccccac gggaagacga cgatgtcccc
     1801 gcaactgtgg gatcggcagc tccgtgccgg cgcctggggg ctcactgccg cctcggcgca
     1861 gcaggcccag gtgatgcgtg gcgtcggcgt gcgccgggtg atgatcgcca acgaactcgt
     1921 cgacccggga tcgatccgat gggcggctgc ctgcctggag gatccggatc tcgagctgtg
     1981 gtcgtgggtc gattcccccg acgcggtgtc cttgctcgaa gcagggatgc ggggatccgg
     2041 tgtcgggggg gcccatcgcg tcctgatcga gctgggtcac ccgggcggtc gcacgggcgc
     2101 ccgatccgtg gccgacgcga tcgaggtcgc cgcttcggtc gcggcctcgc cggtgctgga
     2161 gctgcgtggg gtcgccggat acgaaggcac ggtctgcgcc gaacgacgcc ccgagtgcct
     2221 ggacgccgtc cggcggtacc tcggcgatct ccacgccctg tcggtcgcgg tgctcctcga
     2281 ggggttcatc gatgaacggc acgagatgct cgtgaccgcc ggcggcagca cgttcttcga
     2341 tctcgccgcc gaggcgctga cccagctccg cccggagcac gccggcggcg tccgggtggt
     2401 gatccggccc ggatgcaccc tcacgcacga ccatggcttc tacgccgagc ggtcgccgtt
     2461 cgccgcggtg gatgatccgg cggggcggtt ccatcccgcg ctcgaactgt gggctgcggt
     2521 ctcgtcgctg cccgaacccg gtctcgcgat cgtgggtttc ggacgacgtg acacgtcctt
     2581 cgaccagggc ctgcccgtgc cgctctcgat ccagggtggg gacggcaccc ggcgcgatgc
     2641 ccgaggcatc acggtggacc gcgtcgtgga ccaacacgcg ttctgccggg tggacgaggg
     2701 ggccgacctc gccgtcggcg aggtcatccg tttcggggtc tcgcacccat gcaccgcgtt
     2761 cgacaagtgg cgggtcatgc ccgtcctcga cgatgacgat gtcgtggtcg aggccgtggc
     2821 gacgtatttc tgatcgcctc acgggcgctc acccggtcga gggaagcctt cggtgtcgcc
     2881 gatcaccccg gcgaccgcag cgccgcagtg ggcaccctgg acgagtgcat cggtcgcgtc
     2941 ggcgccgctg aggcgagcgg cgaggtagcc ggcgttgaac gcgtcaccgg cccccacagg
     3001 gtcgacgtcg ggcaccggcg cgggggaatg ttcgtgccat cgcccggccc gatcgagggc
     3061 accggcgccg tccgaccctc gtttgaccac gacctccgca ggaccgagct cgaggatgcg
     3121 ccgtgcgagc gcctcgccgc ggaccgtgac gtcgtcctca cccaccaggt gccgcagttc
     3181 gtgttcgccg ccgagggcga ggtcgcagtt cgcgatcagc gggagcacga gttcgcggcc
     3241 gcggtccgac ccccacaggc cgggtcgaag gtgggggtcg accgctcgca atcccgtggc
     3301 ccgcttcagc agggccagcg ccgcctgctg cggttcggcg ccgagcaatc cggtgatgcc
     3361 cgtgaccatc accgcacgag cttccgcgac gcggacctcc tcgagatcgt cggccgacag
     3421 gatcgaggcg gccgatcccg cccgccggta ctccacacaa ccgtggcagt cgcggatcat
     3481 cacgccggtg gggcggtggt cgtcccgtcg tgtccaacgc aggtcgatgc cctcgtcgtc
     3541 cagggcccgg tggatgcgct cgcccacgac gtcggtgccg agccggctca cccaggcggt
     3601 acggagcccc aggcgtgaca gccccaccag ggtgttggtc tccgcgccgc cgtgggtgac
     3661 gtggaaccgc gtcgccgatg cgagcggctc gtcgatggcc accaacgaca gcatcgtctc
     3721 cccgagcgcg atcacgtcgt atggcgttgt caccgcggct ccggccgggc gtccctcgcg
     3781 gccgacaccg cggccgcggc gcggtcacgg atgcgatcca ggtcgtcgcc cgaccgtggc
     3841 ggtccggcgc ccgtcaacgt cgtgcccatg ccgacgcagg cagccccagc cgcgaggtaa
     3901 gcaccgatgt cgtcgatgcc gatccccccg gtcggcacga accggacacc gtcgaacggg
     3961 cctcggaggg cgcggagatg gcccggcccg cccgtcgagg ccgggaacag cttgatcgtg
     4021 ccggcgccga gcgacctcgc gagctggacc tccgtggggg agaaggcgcc ggggaccggc
     4081 gtcaggccca gagcgagcgc ggtggtgatg gcctcgggga tcacggcggg gctcaccacg
     4141 aaccgggcac cggcatcggc ggcgcgacgg acctcgtcgg ccgagagcac cgtgccggca
     4201 ccgatgaacg tcccggcctc cgaggcgcga cgcaccgcgt cgagcgcgcc gggggtgtcc
     4261 agcgtgacct cgagcatctc gatgccaccg tccatcaggg ccgcgaggat cgggtccagc
     4321 tcggcaggct cgtggaaccg gacgatggcc aggatgtcgc cccggtcgag ggctgtctcg
     4381 tcgttcgtgg gaacctcccg cgacccgggc ggccgccgcg gccggggatc gtgccgcctc
     4441 gttgtcttga ctcccctcga gggcgcagcg tagcatccgg ctcaatagtt aggaaagttt
     4501 cctttgagct gggagggatc gcgcacgagc ctcgtccaga cctccgtcgc cggaaccccc
     4561 agcctgctcc gcgtgatcaa cgagcgcacg gtgctggaga gcgtccgtcg cgtcggcccg
     4621 gtgtcccgcg cccagatcgc ccgcgagacc gccttgtcca agccgaccgt ctcccaggcg
     4681 ctcaccggtc tgctccgcgc caagctggtg cgtgaggcgg gccgctccag cgggggcaag
     4741 ggcccgaccg cggtcctgta tgagctcaac ccgcatgcgg gctgggtcgt ggggatcgac
     4801 gtggggcgtc actgggtgcg tgccgccatc gccgatgtca ccggcgagtt cgtcgcccgc
     4861 cgggacgagc gggcccgcgt caagagcgcg cgaaccctga tcacccagat cggtgcggtg
     4921 gcccacggcc tggcgggcga tgccgggatc cgttggaagc aagtgacgtt cgccaccgtg
     4981 ggaagccctg gcgtgctgga ggccgaccga ggacaggtcg ccttcgccta cgcgctcccg
     5041 gggtggggac gtcaggggct ggtcgaagcc gtgcagcagg aactcggcac gaagatcgcg
     5101 ttcgagaacg acgtcaacct cgcggcgctc ggcgagcagt ggcacggcat cggcaagggc
     5161 gtcgacaact tcgtctatct ccatctgggg actggcgtgg gcatggggct cgtcctcaac
     5221 ggtgagcttt atcgggggag cggcggcgct gccggcgagg tgggatacct ccccttggcc
     5281 ggaaccgatc tgcgtgatcc atcgagccgt cgtcggggcc cgctggatgc ggcgggaagc
     5341 gccgccggcg tcgtcgcgac cgcgcgtcgc ctggggatga cgccgccgct caccgcgaag
     5401 aaggtcttca cggaagcgag gaagggcgat gccaaggcca agcgggtcgt gcgcacggag
     5461 gccgcccgga tcgccctggc gatcgccgcg atctcctcgg tcctcgatcc cgaactcgtg
     5521 atcctgggcg gcgggatcgg tggcaacggc gacctcctgc tcgagcccgt cgagctggaa
     5581 ctccgcgaac tgtcgccctt ccggccccgg atcgaggttt cgaccctggg agaggaagcc
     5641 acgctgcacg gctcggtgtc gatggccctg caggccgcgc acgatcacct gttcgaacga
     5701 gggaaggtgt ccgcatgaaa cctcgagacg tacgaagcaa cgtagggaac ggttggatgg
     5761 atgggttcgt ggatgatccg ggctcggctc ggggagggag ggcacgatga agcgttggtt
     5821 ggtggcgttg gtcaccttcg cgctcgtgtc ggcagcgtgc acggcgggtg gcggcgacga
     5881 cgaaccttcg atcaacgccg acgcgagtgc ttcgcacgag ccggtcacga tcgagatgtg
     5941 gagcgcatgg acgtcgaagg ctgaactcga agacttcgac aagatcttcg cctcgttcga
     6001 ggaggcgtac ccgtggatca ccgtgaagag cgtcggcaac gtcgacgaca cgaagatgct
     6061 cgcggcgatc aacagcggga ccccgcccga cgcggcgctg tcgttcagtt tcgacaacgt
     6121 gggcaagttc tgt
//
LOCUS       DATDBZ010000020        10669 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2051555, whole genome shotgun sequence.
ACCESSION   DATDBZ010000020 DATDBZ010000000
VERSION     DATDBZ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10669
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2051555"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..2243
                     /locus_tag="VIX62_00520"
     CDS             303..2243
                     /locus_tag="VIX62_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858751.1"
                     /translation="MHGIRVLQPVFRATRFPLVVVLMLVVTAATHPVLASNDVSHGNV
                     MVSPNPANGITPDVLDGQVRSMDRIGTSIVVGGSFTRVQLNGGPILNRPFLFAFDVNT
                     GAISKTFTPNVDAKVETVESADDGQHVFIGGYFGSVNGQSQRRVAKLDVQTGALAPGF
                     SVDVDNGERVMDMSVAGDRLFVGGQFSRIGGVDRSRLAAVDIATGAVAPGVDFDFEGR
                     QSGGSNRIQRMDVSPDGESLVVIGNFLTVNGQARAQIAKFDIGSATATLDGWATTAFA
                     PQCHPKFDTYMRDIDISPDGSYFIVGTTGAYGGGPGAGVYCDSITRWELNRQGSGQQP
                     TWREYPGGDTTWSVLSTGAAVYVGGHMRWMNNPFAGDRAGEGSVVRQGIAALDPLNGL
                     PLDWHPRRDPRREGVFKFLATGTGLYFGSDSCCIGGERHERLAFFPLAGGASIPAPTA
                     VTLPSTMYQLPQSGGGAHLTRRSFDGSSFGPSKSVKTPGVDWTTARGAFFTNGHLYSG
                     GNNGNLVRRKFNGTDLGPAQVVDLNGLSATRFPVSNITGMFLSGGLLYYTVSGDSRLF
                     YRYFTHQTPIVGAETFIAGSGGIDWSSVQGMTRAEGRIYFSRTNGSLYRVNFANGAPV
                     AGTEQLVDGSVDWHSRGLFFRG"
     gene            2254..3765
                     /locus_tag="VIX62_00525"
     CDS             2254..3765
                     /locus_tag="VIX62_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY5858752.1"
                     /translation="MSRFRPTAIAVLAIVISQLTITPVLARPLGDVAPARPARPNIVL
                     ILSDDQRVDMMGAMPTVRSRLIDRGVSFRNAYVVDPLCCPSRATILRGQYSHTTRIYG
                     ISRPYGGAGPFKDRGLDRSTVATWLQGAGYRTAMIGKYLNGYDSTSSVEPGWSVWNAL
                     VDTTEQDQYYGYRLNTNGHTTSFGNSPEDYSTDVLAARADDFIRATPAKKPLFLYFAP
                     TAPHLPAEPAPRHLDDPRCSDAANSGYVPGQTGPPPSFNEADLDDKPGWVGRKDLLDA
                     AAQSETWIRICRTLLAVDDAVRTIVTALADTGRINDTLIVYTSDNGLLRGEHRLIYKK
                     AAYEESIRVPMVIRYDGMTKHGVIEDRFALNVDLARTFADAAGVEPGVHQQGRSLLRL
                     LNGSATDWRKQFLVEHADPPGGAGGDRYVPTYCALHGSRFVYMALTTGEEELYDLRTD
                     PYQLQNLLSGPSGSWPPGVSARRDDLYARLFLGAKGAKPLCFPLPPDYSPPPA"
     gene            3959..4459
                     /locus_tag="VIX62_00530"
     CDS             3959..4459
                     /locus_tag="VIX62_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY5858753.1"
                     /translation="MRKSTLAIVAFGTAAILAVGGAFAMADAPQHVKARLNGYAEVPT
                     NSTTGNGEFEATIDGNSIDYRLEYGELEGGDVLFAHIHLGRRATNGGVAAFLCGGGGK
                     PACPQSGTVEGTIVPADVVGPAVQGLDVGEFRELVRAIRRHATYVNVHTEVFPSGEIR
                     GNVIAS"
     gene            4575..5618
                     /locus_tag="VIX62_00535"
     CDS             4575..5618
                     /locus_tag="VIX62_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020113242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5858754.1"
                     /translation="MEYRILGGTGIRVSTHCLGTMAFGAWGNEDHDECIRTVHQALDA
                     GINFVDTADMYSDGESEEIVGKAIVGRRDEVVLASKFHNPMGSGPNDRGNSRVWIMRA
                     VEASLRRLGTDHLDLYQVHRPEEETDIEETLAALTDLQRAGTIRAFGTSTYAGWQLVN
                     AQSVSERRGLARFRTEQPPYSIFVRHVERDVFPVAQEYGIGVLVWAPLGRGWLTGRYR
                     RGNFDRSPEARATRNRERGGPIAAQFDEDRPEIQRKLDVVEELAAIADGAGISMTHMA
                     VSFTLAHPAVTSAIVGVRTPRQIEELIAGADLRLDEATLDAIDAVVPPGSTLDENDMG
                     FAPWWMEAARRRR"
     gene            complement(5666..6460)
                     /locus_tag="VIX62_00540"
     CDS             complement(5666..6460)
                     /locus_tag="VIX62_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5858755.1"
                     /translation="MATRTSYEPGTFSWVELATTDRDDAKRFYSGLFGWECEDESRLG
                     GDVSTIARLDGEAVAGITDQPASQGREVPSTWVSYVTVASADESATRAGELGGTVHTR
                     PFDIGDAARVAVVADPTGAAIGLWEPRSSIGAERVNGSGCLTSNELATDDVDAASKFY
                     RELFGWGIEEVDTGGGPRYWLIHGEGALEGRNGGVRELGRPGEGMTPNWMPYFTTESA
                     GKALARADELGGTTLMPATQIPAGIIAAVRDPQGAVFSIFEGDVDD"
     gene            complement(6475..6786)
                     /locus_tag="VIX62_00545"
     CDS             complement(6475..6786)
                     /locus_tag="VIX62_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY5858756.1"
                     /translation="MTHVLLNKLTAKPGRREQVVQNLIESGTLFDDNAACVMYLVTES
                     SDSPNDIWVFDLWTSEEAHSQALQAPELQPSIAETVPLLEGMPEQIEVQARGGKGLPT
                     G"
     gene            complement(6834..7172)
                     /locus_tag="VIX62_00550"
     CDS             complement(6834..7172)
                     /locus_tag="VIX62_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5858757.1"
                     /translation="MFDVEASDSEGVRRIVLVGEFDLAAMPTAETSLAEATANGYGAL
                     EIDLRALTFLDSSGVRSLMTTVDRCRDRGVPLTIIPGPRGVQRVFEITGLVDALPFST
                     PTDEADGERT"
     gene            7270..7668
                     /locus_tag="VIX62_00555"
     CDS             7270..7668
                     /locus_tag="VIX62_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5858758.1"
                     /translation="MSEISTQLPPLAEAPAMARHILAEFVDETLPPEMLEDAALLTSE
                     VVTNSVQHAGLSTNEGIGIELVLSDDSLRVSVTDPGPGFEPDLDAPAPEQEMGGWGLA
                     LVDRVSDRWGVIRNRPNVVWFELDRRTTIT"
     gene            complement(7685..9238)
                     /locus_tag="VIX62_00560"
     CDS             complement(7685..9238)
                     /locus_tag="VIX62_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY5858759.1"
                     /translation="MPERYDVIIIGTGAGGGTLAHTLAPSGKKILLIERGDFLPRERE
                     NWDPNQVFIEGRYISPDTWYDPDGKTFQPQVHYYVGGATKMYGAALYRLRPQDFGEIQ
                     HIDGLSPAWPLSYDDFEPWYSRAEQLYQVHGNHGEDPNEGHWSQQYPWPAVSHEPRIQ
                     QLADDLTAAGYQPYHAPCGIMLNEAHRAKSACIRCTWCDGYPCLVHAKSDAESIAVRP
                     VLTSPNVTLLVNTEVVKLETDPTGRSITGVVVTREGERETYEADIVSLSAGASNTAKI
                     LLSSANDQHPNGLANGSDQVGRNYMFHNSKAVVALSKEPNDTVFQKTLAINDFYLGAD
                     DYGWPVGQIQMVGKSNAEAMKGEEPMLTKLAPHWSLAETARHVVDFWLTTEDLPQADN
                     RVTLADDGNVRLAYRSTNDEEAGRLYHELKIILNHAGMADHHVLHKNFYMHMSIPIAG
                     VAHQAGTCRFGSDPASSVLDANCKAHELDNLYVVDTSFFPSIGAVNPALTAMANAIRV
                     GEHLTERMA"
     gene            complement(9231..10568)
                     /locus_tag="VIX62_00565"
     CDS             complement(9231..10568)
                     /locus_tag="VIX62_00565"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5858760.1"
                     /translation="MTKKHKKKKDKKKKKDAAKTTEVVIVGAGFAGIGCAKELAKRHD
                     VHVTLLDKHNYHQFLPLLYQVATYQLSPTDVAMDLRQEFRKHPRVSVKMGEVASIDPS
                     TRSVALADGGTFSGDYIVLAAGSQANFFDTPGAEHVFPLYSLDDAERLRSRLLAIFED
                     ADRDPSLVEKGALNVVVVGAGATGTEVAGAIGDLVRDVLPSEFHDLAVDKARVIVVDL
                     GDAVLGPFSPKAHAYASKVLLGLGVELRLGTSVKEIARDHVVLSDGSTILTHCVVWGG
                     GLKAAPLAGASGLKQGRGGRIDVEPDLTVADHPGIYAVGDIANTPDEQGATFPQLGSV
                     ALQAGSTVAANIIADIEGKPRETFDYHDKGIMAMIGRGAAVAEVGEHRHELHGAIAFA
                     AWLGVHAWLMSGVRQRIDAFVSWGWDYFGKSRAPQILDRSDAAHIDWGNDDDA"
     gene            complement(10565..>10669)
                     /locus_tag="VIX62_00570"
     CDS             complement(10565..>10669)
                     /locus_tag="VIX62_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEY5858761.1"
                     /translation="IEPMTCPPNALRTGTDLIRLEPGSSWTSRWGIRA"
BASE COUNT         1736 a         3519 c         3563 g         1851 t
ORIGIN      
        1 acgtccaggg agcgacgctc ggcaagaacg tggcgaattg ggtggcatcg cactacctca
       61 agccctcgta ttgatcgacg gagttccctg atgatcgcca cgccgccgga caggcggcgt
      121 ggcgatccgt catccaggca acgatgggat gagcgacatc ttcccgacgc aacggtcgaa
      181 ggtcccgaga ccccatgggg cgttcagccg tcgctcatct ttccgattga ccgcttttcg
      241 tctcgatgca acgttggagt cactgagggg cccggcaccg cggggggacg tcaggggggt
      301 acatgcacgg gatcagagtg cttcagccgg tattccgagc gacgcggttc ccgctcgtgg
      361 tcgtcctgat gctggtcgtg acggccgcca cgcaccccgt cctcgcgtcg aacgacgtct
      421 cccacgggaa cgtcatggtg agcccaaatc ctgcgaacgg gatcaccccc gatgtcctcg
      481 atgggcaggt tcggtcgatg gatcggatcg gcacgagcat cgtcgtcggc ggaagcttca
      541 cccgggtgca gctgaacggc ggcccgatcc tgaacaggcc cttcctgttc gcgttcgacg
      601 tgaacacggg agcgatcagc aagacgttca caccgaacgt cgatgcgaag gtggagacgg
      661 tggaatccgc cgacgacggc cagcacgtgt tcatcggtgg ctacttcggt tccgtgaacg
      721 gtcaatcgca gcggcgcgtc gcgaaactcg atgtgcagac cggcgccctc gcgcccgggt
      781 tctcggtcga cgtggacaac ggcgaacggg tcatggacat gtcggtcgcg ggggaccgct
      841 tgttcgtcgg cgggcagttc agcaggatcg gcggcgtcga tcgctccagg ctggcggccg
      901 tcgacatcgc gaccggcgct gtggccccag gcgtggactt cgacttcgaa ggaaggcaga
      961 gcggcggcag caaccgcatc cagcgcatgg atgtgtcgcc tgacggcgag tcgctcgtgg
     1021 tgatcgggaa cttcttgacc gtcaacggcc aggctcgtgc gcagatcgcg aagttcgata
     1081 tcggctcggc gaccgccacg ctcgacgggt gggcaacgac cgcgttcgcg ccgcaatgtc
     1141 atcccaagtt cgacacgtac atgcgcgata tcgacatctc acccgacggc tcatacttca
     1201 tcgtcggcac gaccggcgcg tacggtggcg ggccgggcgc aggcgtctac tgcgactcga
     1261 tcacgcgctg ggagttgaac cggcagggtt ccggtcagca acccacgtgg cgcgaatacc
     1321 ccggcggcga cacgacgtgg agcgtgctct cgaccggcgc ggccgtgtac gtgggtggcc
     1381 acatgcggtg gatgaacaac ccgttcgccg gcgatcgcgc cggggaaggc tcggtcgtgc
     1441 gccaggggat cgcggcgctc gatccgctca acggcctgcc cctcgactgg cacccccgcc
     1501 gcgatccacg gcgagagggt gtgttcaagt tcctggcgac gggcacgggg ctctacttcg
     1561 gcagtgacag ctgctgcatc gggggagagc gccacgagcg cctcgcgttc ttcccactcg
     1621 cggggggagc ctcgatccct gcgccgaccg cggtcacgtt gccttccacc atgtatcaac
     1681 tcccgcagtc gggcggcggg gcacatctga cccgcagatc gttcgacggt tcgtcgttcg
     1741 gcccctcgaa gagcgtgaag acccccgggg tggactggac gacggcgagg ggcgcgttct
     1801 tcaccaacgg ccatctctat tcgggcggca acaacgggaa cctcgtccgc cggaagttca
     1861 acggcaccga cctgggaccc gcgcaggtcg tggacctgaa cgggctctcg gcgaccaggt
     1921 tcccggtctc gaacatcacc ggcatgttcc tgtcgggcgg gctcctctac tacacggtct
     1981 cgggcgacag taggctcttc tatcgctact tcacccatca aacaccgatc gtgggggctg
     2041 agacattcat cgcgggcagc ggcgggatcg actggtccag cgtgcagggc atgacccgcg
     2101 ccgaggggcg gatctacttc tccaggacca atggcagcct ctaccgggtc aacttcgcga
     2161 acggggcgcc ggtcgccggt acggagcagc tggttgatgg ctccgtggac tggcactcgc
     2221 gtggattgtt cttcaggggc tgagccgaca ccgatgtcgc gtttccggcc gaccgcgatc
     2281 gcggtcctgg cgatcgtgat ctcccaactg acgatcaccc ccgttctggc gcgcccgctg
     2341 ggcgatgtag cgccggcccg tccggctcgc ccgaacatcg tcctgatcct gtccgacgac
     2401 caacgggtgg acatgatggg ggcgatgccg accgtccggt cgaggctgat cgatcgaggt
     2461 gtgtcgttcc ggaacgcgta cgtggtggac ccgctgtgct gcccgtcgcg ggcaacgatc
     2521 ctgcgtgggc agtattcgca caccacgcgg atctacggca tctcgcgccc gtacggcggc
     2581 gccggaccgt tcaaggaccg cggtctggat cggtccacgg tcgcgacatg gctgcagggg
     2641 gccggctatc gcaccgcgat gatcggcaag tatctcaacg gctatgactc gacctcgtcg
     2701 gtcgagccgg gctggagcgt ctggaacgcc ttggtcgaca cgaccgagca ggaccagtac
     2761 tacgggtacc ggctcaacac gaacggccac acgacctcgt tcggcaactc gcccgaggac
     2821 tactcgaccg acgtgctggc ggctcgagcc gacgacttca tccgggcgac gcctgcgaag
     2881 aagccgctct tcctgtattt cgcccccacg gcgccccacc tgccggcgga gccggcgcct
     2941 cgtcacctcg acgatccgcg ctgcagcgac gctgcgaaca gcggctacgt gcccggacag
     3001 accggtccac ctccctcgtt caacgaagcg gacctcgatg acaaaccggg ctgggtgggc
     3061 aggaaggacc tcctggacgc ggccgcccag tccgaaacct ggatccggat ctgccgcacg
     3121 ttgctcgccg tggacgatgc ggttcgcacg atcgtgacgg ccctcgccga taccggacgg
     3181 atcaacgaca ccctgatcgt ctacacgtcc gacaacggac tgctccgggg cgaacaccgc
     3241 ctgatctaca agaaggccgc ctacgaggaa tcgatccggg tcccgatggt gatccggtat
     3301 gacggcatga ccaagcacgg cgtgatcgag gaccggttcg cgctcaacgt cgacctcgcc
     3361 cggaccttcg ccgatgcggc gggcgtcgag ccgggcgtcc atcaacaagg caggagcctg
     3421 ctccggttgc tcaacggctc ggcgaccgat tggcggaagc agttcctggt cgagcatgcg
     3481 gaccctcccg gtggcgcagg gggagaccga tacgtaccga cctactgcgc gctccacggg
     3541 tctcggttcg tctacatggc cctcaccacc ggcgaagagg agctctacga tctgcgaacg
     3601 gatccgtatc agctccagaa cctgctctcc ggaccttcgg ggtcgtggcc gccgggcgta
     3661 tcggcgcgac gtgacgacct gtacgcgcgg ctgttcctcg gagccaaggg tgcgaagccg
     3721 ctgtgcttcc ccctgccgcc cgactactcg ccacccccgg cgtagccagc agcaccgtcc
     3781 ccgagcatcc tgccctgggc gcagcagggc cgaaccacgc tgaaggccga gacctccctc
     3841 tggagatgaa cccgatggtg cgccgttcgt acacctatcg aggaggggtc gatgctggac
     3901 gtcgccggga gggtcgcccg ccggccaggg catcgacgga gagatgggag gaacgcagat
     3961 gaggaagagc acgctcgcga tcgtcgcctt cggcacggcg gcgatcttgg cggtcggcgg
     4021 cgcattcgcc atggccgatg ccccgcagca cgtgaaagcc cgcctgaacg gatacgccga
     4081 ggtgccaacc aactcgacca cgggcaatgg cgagttcgag gccacgatcg atggcaactc
     4141 gatcgactac cgtctcgagt acggggagct ggagggtggc gatgtgttgt tcgcccacat
     4201 ccatctgggt cgccgagcca cgaacggcgg cgtcgccgcg ttcctgtgcg gcgggggcgg
     4261 caagcccgca tgcccgcagt cgggaacggt ggaaggcacg atcgtccccg ccgacgtggt
     4321 cggtccggcc gtacagggcc tcgacgtcgg tgagttccgt gagctcgttc gagcgatccg
     4381 gcgtcacgct acctacgtca acgtccacac cgaggtgttc cccagtggcg agatccgcgg
     4441 caacgtgatc gcgtcctgaa acgacggagc cgaccggtga cggccccacc gggacggctc
     4501 cgagccaggc agttcccccc atcgaccgtt cccacaccct tgaccgtcgc ggccagcgga
     4561 cggagggttg agcgatggaa tatcgcatcc tcggaggaac cggcatccgc gtgagcaccc
     4621 actgcctggg cacgatggcc ttcggcgcgt ggggcaacga ggatcacgac gagtgcatcc
     4681 gcacggtgca tcaggcgctg gacgccggga tcaacttcgt cgataccgcc gacatgtact
     4741 cggacggtga gtccgaggag atcgtcggca aggccatcgt gggccgtcgg gacgaggtcg
     4801 tgctggcgtc gaagttccac aaccccatgg gttccggtcc gaacgatcgc ggcaactccc
     4861 gtgtgtggat catgcgcgcc gtcgaagcga gtctgcgcag gctgggaacg gaccatctcg
     4921 acctctacca ggtgcatcgc ccggaggagg agacggatat cgaagagacg ctggcagcgc
     4981 tgaccgatct ccagcgtgcc ggcacgatcc gggcgttcgg gacgtccacc tacgccggtt
     5041 ggcagttggt gaacgcccaa tcggtgtccg agcgacgcgg tctcgcgcgc ttccggaccg
     5101 agcagccccc atattcgatc ttcgtgcgcc acgtcgagcg cgatgtgttt cccgttgccc
     5161 aggagtacgg gatcggggtg ctggtgtggg cgcccctcgg tcgtgggtgg ctgacgggcc
     5221 gataccgccg cgggaacttc gatcgctcgc ccgaagcgcg tgccacccgc aatcgcgaac
     5281 ggggtgggcc gatcgccgcc cagttcgatg aggaccgccc ggagatccag cggaagctcg
     5341 acgttgtgga ggagctggcc gccatcgcgg acggtgcggg gatctccatg acgcacatgg
     5401 cggtgtcgtt caccctggcg caccccgccg tgaccagcgc gatcgtcggt gttcgaacac
     5461 cccggcagat cgaggagctg atcgccgggg ccgatctgcg gctcgacgag gccacgctcg
     5521 atgcgatcga cgccgtcgtg cctcccggct caacgctcga cgagaacgac atggggttcg
     5581 caccgtggtg gatggaagcc gcccgccggc gccgataggc ggatcgagaa gatgcgagaa
     5641 gagcggagct ggctacgcgc tgggctcagt cgtcgacgtc gccttcgaag atcgagaaga
     5701 cggcaccttg agggtcccga accgcggcga tgatgccggc ggggatctgc gtcgcaggca
     5761 tgagcgtggt gccgcctagc tcgtcggctc gtgccaacgc cttgccggcc gactcggtag
     5821 tgaagtacgg catccagttg ggcgtcatgc cttcccctgg gcggcccagc tcgcgcacgc
     5881 ccccgttacg gccctcgagg gcgccctcgc cgtggatgag ccagtagcgc ggcccgccgc
     5941 ccgtgtcgac ctcctcgatg ccccacccga acagctcgcg gtagaacttc gaggcggcgt
     6001 cgacgtcgtc ggtcgcgagt tcgttggagg tcaggcatcc ggagccgttc acgcgctccg
     6061 cgccgatcga actccgaggc tcccagagtc cgatcgcggc cccggtcgga tcggcgacga
     6121 ccgccaccct cgccgcatcg ccgatgtcga acggtcgcgt atgcaccgtg ccgcccagtt
     6181 cgccggcacg cgtcgcggac tcgtccgcgc tcgcgacggt cacgtagctc acccaggtgg
     6241 atggaacctc tcgcccctgg gatgcgggct ggtcggtgat ccccgcgaca gcctcaccat
     6301 cgaggcgggc gatcgtggag acgtcacctc ccaggcgcga ttcgtcctcg cactcccagc
     6361 cgaacagccc gctgtagaag cgcttcgcgt cgtcgcgatc cgtggtggcg agctcgaccc
     6421 aggagaaggt gccgggctcg tagctggttc tcgttgccat cgtcgatacc tcgctcagcc
     6481 tgtggggaga cccttcccgc ctcgagcctg tacctcgatc tgctcgggca tcccctccag
     6541 caacggcacg gtctcagcga tggagggctg cagttcgggg gcttggagcg cctgcgagtg
     6601 tgcttcctcg ctcgtccaca gatcgaacac ccagatgtcg ttcggggagt cggatgactc
     6661 ggtcacgaga tacatcacgc acgccgcgtt gtcatcgaac agcgttcccg actcgatcag
     6721 gttctggacg acctgttcgc gcctgcccgg cttcgccgtg agtttgttga gcaatacatg
     6781 ggtcatgtgg tcgcggtacc caggctgact cgcgacaaac atgccgggtg ttgctacgtc
     6841 cgttcgccgt ccgcttcgtc cgttggcgtc gagaacggca gggcatcgac cagcccggtg
     6901 atctcgaaca cccgctggac cccccggggg ccggggatga tggtgagcgg caccccacga
     6961 tcgcggcacc gatccacggt ggtcatcagt gaacggacac cggaggaatc caggaacgtc
     7021 aaggctctga ggtcgatctc gagcgctccg tagccgttcg cggtcgcttc agcgagcgag
     7081 gtctcagccg tcggcatcgc cgcgagatcg aactctccca cgaggacgat cctgcggaca
     7141 ccttccgagt cggatgcctc gacgtcgaac atcgttgcct ccttccaggg cgatcaaggg
     7201 tgcctatttt agcgaagagc gggtatgcgt cgtaccgagg agtcgtccac gaccaccgaa
     7261 ggcacccaga tgtccgagat ctcaacccag cttccccccc tcgccgaggc gccggcgatg
     7321 gctcgccaca tcctcgctga gttcgtcgac gagaccctcc cgccggagat gctggaggat
     7381 gcagcactcc tgaccagcga agtcgtgacg aacagcgtcc agcatgcggg gctttcgacg
     7441 aatgagggga tcggcatcga gctggtgttg tcggacgact ccctgcgggt gagcgtgacc
     7501 gaccccggac caggcttcga acccgacctc gacgcaccgg cgccggaaca agagatgggc
     7561 ggttggggtc tggccctcgt ggatcgggtc tctgatcgct ggggcgtcat ccggaaccgc
     7621 ccgaacgtcg tgtggttcga actcgaccgc cggacgacga tcacctgacc tcgaagctcg
     7681 ttcgctaggc catccgctcg gtcagatgtt caccgacccg gatggcgttc gccatggcgg
     7741 tcagcgccgg gttcaccgct ccgatgctgg ggaagaagct cgtatccacg acgtagaggt
     7801 tgtcgagttc gtgagccttg cagttggcgt caaggaccga gctcgcgggg tcggagccga
     7861 accggcacgt gccggcctgg tgcgctactc ccgcgatcgg gatgctcatg tgcatgtaga
     7921 agttcttgtg aaggacgtgg tgatcggcca tgccggcgtg gttcaggatg atcttcagct
     7981 cgtgatagag gcggccggcc tcctcgtcgt tcgtcgaccg atacgccaac cggacgttgc
     8041 cgtcgtcggc gagggtgacc cggttgtccg cttgcgggag atcctcggtg gtcagccaga
     8101 agtccacgac gtgccgcgcg gtttccgcga ggctccagtg cggcgcgagc ttggtgagca
     8161 tcggctcctc acccttcatg gcttccgcgt tcgacttgcc caccatctgg atctgcccga
     8221 ccggccagcc gtagtcgtcg gctcccaggt agaagtcgtt gatggccagg gtcttctgga
     8281 agacggtgtc gttcggctcc ttcgagaggg ctacgaccgc tttgctgttg tggaacatgt
     8341 agttgcggcc gacctggtcg gaaccgttgg cgagcccgtt cgggtgctgg tcgttggcgg
     8401 agctcagcag gatcttcgcg gtgttggagg cgcccgccga cagggagacg atgtccgcct
     8461 cgtaggtctc tcgttcgccc tcccgcgtca cgacgacccc cgtgatcgag cgtccggtcg
     8521 ggtccgtctc gagcttcacc acttcggtgt tcacgagcag cgtcacgttc ggcgaggtca
     8581 ggacgggccg aaccgcgatc gattcggcgt cagacttcgc atgcaccagg cacgggtagc
     8641 catcgcacca ggtgcagcgg atgcacgcgc tcttcgcgcg atgcgcctcg ttgagcatga
     8701 tgccgcacgg cgcgtggtag ggctgatagc cggcggcggt gaggtcgtct gcgagctgct
     8761 ggatcctggg ttcgtgcgag accgccggcc aagggtactg ctggctccaa tgtccctcgt
     8821 tcggatcctc gccgtggttg ccgtgcacct ggtagagctg ctcggccctc gaataccacg
     8881 gctcgaagtc gtcgtaagac agcggccacg cgggggagag gccgtcgatg tgctggatct
     8941 cgccgaagtc ctggggacgg aggcggtaga gcgccgcacc gtacatcttc gtcgcgccac
     9001 cgacgtagta atggacctgc ggctggaagg tcttgccgtc ggggtcatac caggtgtcgg
     9061 gggagatgta gcgcccctcg atgaacacct ggttggggtc ccagttctcg cgttcccggg
     9121 ggaggaagtc cccgcgctcg atcagcagga tcttcttgcc cgacggagcg agggtgtgcg
     9181 ccagcgtgcc tccgcccgct ccggtcccga tgatgatcac gtcgtagcgc tcaggcatcg
     9241 tcgtcgttcc cccaatcgat gtgtgctgcg tccgatcgat ccaggatctg cggtgcgcga
     9301 ctctttccga agtagtccca gccccagctg acgaaggcgt cgatgcgctg acgcacgccg
     9361 ctcatcagcc aggcatggac ccccagccag gcggcgaacg cgatcgcacc gtgaagctcg
     9421 tgccgatgct ctcccacctc ggcgaccgcc gccccgcggc cgatcatcgc catgatcccc
     9481 ttgtcgtggt agtcgaacgt ctcgcggggt ttcccttcga tgtccgcgat gatgttggcg
     9541 gccacggtgc ttccggcctg cagcgccacc gatcccagtt gcgggaacgt ggcaccctgc
     9601 tcgtcggggg tgttggcgat gtcgccgacc gcgtagatcc ccgggtggtc cgccaccgtg
     9661 aggtcgggtt cgacgtcgat ccgtccaccg cgcccctgct tgaggcccga cgcgccggcg
     9721 aggggggcgg ccttcaaccc tccgccccag acgacgcagt gtgtgaggat cgtggaaccg
     9781 tccgacagca ccacgtgatc gcgtgcgatc tccttcaccg acgtccccaa ccggagttcg
     9841 acgccgaggc cgagcaggac cttggaggca tatgcatggg ccttcggcga gaacgggccc
     9901 aggaccgcgt cgccgagatc gaccacgatc acccgggcct tatcgacggc gaggtcgtgg
     9961 aactccgacg gcagcacgtc gcgcaccagg tcgccgatcg cccctgcaac ctcggtaccg
    10021 gtggcgcccg ccccgacgac cacgacgttg agcgcaccct tctcgacgag cgacgggtcg
    10081 cgatcggcat cctcgaagat ggccagcagt cgtgaccgga ggcgttcggc gtcatcgagt
    10141 gagtacagcg ggaacacgtg ctcagcaccg ggcgtgtcga agaagttggc ctggctccct
    10201 gcggcgagga cgatgtagtc cccggagaat gtcccgccgt cggccagcgc gaccgatcgc
    10261 gtcgacggat cgatcgaggc cacctcgccc atcttgacgc tgaccctcgg gtgcttccgg
    10321 aactcctgac gcagatccat cgccacgtcg gtaggcgaga gttgataggt cgcgacctgg
    10381 tacaggagcg gcaggaactg gtggtagttg tgtttgtcga gcagggtgac gtggacgtcg
    10441 tggcgtttcg cgagctcctt cgcgcagccg atgccggcga acccggctcc cacgatgacg
    10501 acttcggtcg tcttcgcggc gtccttcttc ttcttcttgt ccttcttctt cttgtgtttc
    10561 ttcgtcatgc gcggatcccc caacgtgacg tccacgatga tccgggttcg agccggatca
    10621 ggtcggtgcc ggttcgcaat gcgttcggtg ggcaggtcat cggttcgat
//
LOCUS       DATDBZ010000021         2678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1028907, whole genome shotgun sequence.
ACCESSION   DATDBZ010000021 DATDBZ010000000
VERSION     DATDBZ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028907"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VIX62_00575"
     CDS             <1..932
                     /locus_tag="VIX62_00575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY5858762.1"
                     /translation="FDYRPMLAPLAPLVRSADLAICHLETPLSGPGVPISGPPRFAAP
                     HQLATAIRSTGYDGCSTASNHSLDQGKAGIRTTLDALDRVGLGHTGTARTRAESRRIA
                     RYRIDGAEIAHLAFSYGFNGLRPRHAWMANRIRAATIVRRATAARETGASLVIVSLHW
                     GTEFRHVPDPSQQRLARRIMDSGVVDLIIGHHAHVVQPVRRVHDRWVAYGVGNSLTGM
                     TAADSIIDAQDGMVLIATFAQNTDGWSLARMRFAPTWVQPQRFLVHLVGPALDRRRLP
                     SPILAALRRSWRRTVGAVDASSLGVIPFRGRHV"
     gene            complement(985..1248)
                     /locus_tag="VIX62_00580"
     CDS             complement(985..1248)
                     /locus_tag="VIX62_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858763.1"
                     /translation="MEDELISAAEDAATDALEEAGAASELHADAAAKAAAAVELEAAA
                     EELMSEAVVEEAVAEELDDMAVGDAVVAEALIEEAEAEASDEA"
     gene            1340..2011
                     /locus_tag="VIX62_00585"
     CDS             1340..2011
                     /locus_tag="VIX62_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein"
                     /protein_id="HEY5858764.1"
                     /translation="MKDAPLLRGNVLARMVALFAGLIVIAVAIVSMLESGLGLPPWDV
                     FHMGVAEHTPLSLGNAAIVVGLALIAIAWVAGTAPGFGTVANAIVIGLAIDVLSAIPA
                     VERLSGSALPVRIFLVAVGILLFGVGSALYIGAGFGAGPRDSLMLVLTRRTGWRIGAV
                     RAGIEVIVLAAGLLLGGVAGIGTVALALLVGPSVELSFWALIRARLALPGSRQAQEFG
                     PLDVG"
     gene            complement(2026..2442)
                     /locus_tag="VIX62_00590"
     CDS             complement(2026..2442)
                     /locus_tag="VIX62_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEY5858765.1"
                     /translation="MAEANDWNTNIIEEFRANGGKVGGPFEGAPLLLLHTTGARSGAE
                     RVNPLMYLPDGDRLVVFASKGGAPDNPDWYHNVLANPGVSIEVGDETVDARATVADDT
                     ERDTLFAEQARRYPQFQGYQDGIDRTIPVVILERAS"
     gene            2499..>2678
                     /locus_tag="VIX62_00595"
     CDS             2499..>2678
                     /locus_tag="VIX62_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858766.1"
                     /translation="MVRTAARWILAGALAFAGVMHFVATETFNRLVPPLLPAPTALVW
                     VTGVMEVGFAVALVAT"
BASE COUNT          334 a          888 c          949 g          507 t
ORIGIN      
        1 ggttcgacta ccggccgatg ttggccccgc tggcgccgtt ggtgaggtcg gcagacttgg
       61 cgatctgtca cctggaaacg ccgttgtcag gccctggggt gccgatcagc gggccgcccc
      121 gcttcgcagc cccgcatcag ctggccaccg cgatccggtc gaccggctac gacggatgct
      181 cgacggcctc caaccattcc ctcgaccagg ggaaagcagg gatccgcacg accctggatg
      241 cgctggatcg cgtcgggctc ggacacaccg gcacggcgag gacgcgcgcg gaatcccggc
      301 ggatcgcccg gtaccggatc gatggcgccg agatcgcgca cctcgccttc tcatacgggt
      361 tcaacggact gcgaccccgg cacgcgtgga tggccaaccg gatccgggcg gccacgatcg
      421 tccgtcgcgc gacagcggct cgcgagacgg gtgcctccct ggtgatcgtg agcctgcact
      481 ggggcacgga gttccggcat gtcccggacc cctcacagca gcggttggcg cgcaggatca
      541 tggactcggg cgtcgtcgac ctgatcatcg gtcaccatgc ccatgtcgtg cagccggtcc
      601 gtcgcgtgca cgaccgatgg gttgcctatg gcgtcgggaa cagcctcacc gggatgaccg
      661 cggctgactc gatcatcgat gcccaagacg gcatggtcct catcgccacg ttcgcacaga
      721 acaccgatgg ctggtcactc gcgcggatgc ggttcgctcc gacgtgggtc cagccccaaa
      781 ggttcctcgt gcacctggtc ggtcccgcgc tcgaccgccg ccggctcccc tcgccgatcc
      841 tggctgcgtt gcgccggtcg tggcggcgca cggtgggagc ggtcgatgct tcgtccctgg
      901 gggtcatccc gttccgtggt cgtcacgtgt gagagggcgc cccagcgggg cgccctctct
      961 gcgtgccgga tgatgctcac gcggtcaggc ttcgtcgctc gcctcggctt cggcttcctc
     1021 gatcagggcc tcggcaacga cggcgtcgcc cacggccatg tcgtcgagtt cctcggcgac
     1081 ggcttcctcc accacggctt cggacatcag ttcctcagcg gcggcttcga gttccaccgc
     1141 ggcggcggcc ttcgcggcag catcggcgtg gagttcggag gctgcgcccg cttcctcgag
     1201 tgcgtcggtg gctgcgtcct ctgcagcact gatcaactcg tcttccatct cgtgctcctt
     1261 cctgggtcgg gaggaaggag catagggcca cgcggcgacg cgtgcaggcg tcccggtagc
     1321 ctgccgaggt ggatacccgt tgaaagatgc tccgctgctc cgaggcaacg tcctcgctcg
     1381 catggtggcg ttgttcgccg gcttgatcgt gatcgcagtc gcgatcgtct ccatgctgga
     1441 atcggggctc ggacttcccc cgtgggatgt gttccacatg ggcgtggccg agcacacacc
     1501 gttgtcgctg ggcaatgctg ccatcgtggt cgggttggcg ttgatcgcca tcgcatgggt
     1561 ggccgggacg gcgccggggt tcggcacggt ggccaacgcg atcgtgatcg gcctggcgat
     1621 cgacgtgctc agcgcgatcc ctgcggtgga acggctgtcg ggctcggcct tgccggtgcg
     1681 catcttcctg gtggcggtcg ggatcctgtt gttcggggtc ggttcggcgc tctacatcgg
     1741 cgccgggttc ggcgcgggcc cgcgtgactc attgatgctg gtgctgaccc gccggaccgg
     1801 gtggcgcatc ggcgccgtgc gtgcgggcat cgaggtgatc gtcctggccg cgggcttgct
     1861 gctgggcggt gtcgcaggca tcgggacggt ggcgctggcc ttgttggtcg gtccgtcggt
     1921 cgagctttcg ttctgggcgc tgatacgggc gaggctggcg ctgcctggct cgcggcaggc
     1981 gcaggagttc ggcccgctcg acgtggggta gggggttctt cgggcctaac tcgcacgctc
     2041 caagatcacc acggggatcg tgcggtcgat cccatcctga tacccctgga actgtgggta
     2101 gcgacgagcc tgttcggcga acaaggtgtc ccgctcggtg tcgtcggcca ccgtcgctcg
     2161 ggcatcgacc gtctcatcgc cgacctcgat ggagacaccg gggttcgcca gcacgttgtg
     2221 gtaccagtcc gggttgtcgg gcgcaccgcc tttcgatgcg aacacgacga ggcgatcgcc
     2281 gtcgggtagg tacatgaggg ggttgacccg ctcggcgccc gacctcgcgc cggtggtgtg
     2341 gagcaggagc agcggagccc cttcgaacgg tccaccgacc ttgccgccgt tggcgcggaa
     2401 ctcctcgatg atgttcgtgt tccagtcgtt ggcctcggcc atgtccgttc ctctcgatcg
     2461 cgtcgatggg gtgcgcaacc cgatgctacc gtccgggcat ggtccggacg gcggcgcggt
     2521 ggatcctggc cggcgccctc gcgttcgcag gcgtcatgca cttcgtggcg accgaaacct
     2581 tcaacaggct ggtgcccccc ttgctcccgg cgccgaccgc gctcgtgtgg gtgacgggtg
     2641 tgatggaggt cgggttcgcg gtggccctgg tcgcgacg
//
LOCUS       DATDBZ010000022         2414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_210807, whole genome shotgun sequence.
ACCESSION   DATDBZ010000022 DATDBZ010000000
VERSION     DATDBZ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210807"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VIX62_00600"
     CDS             <1..384
                     /locus_tag="VIX62_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5858767.1"
                     /translation="VLRELTSSGVGADALVGSARDAGERVEVGALSRSVPVPLIVRTE
                     GVDGGSFTTSDGVEARYPAVPAAAAPDDDADAYGAGDSFAAGLTFALGQGTSIIDALT
                     LAARCGAACAAGAGPYARQLTVADI"
     gene            434..2266
                     /locus_tag="VIX62_00605"
     CDS             434..2266
                     /locus_tag="VIX62_00605"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEY5858768.1"
                     /translation="MDIAGFLGRYPPFDALTHEHLAEIAGSVEIEHFAAGVTILQQGG
                     EPATAQYVIRKGAVELLDDGQLLDLLVEGELFGQFSLLAHESPTLTVRAQEDTLCYLV
                     PAEVADAVLGSHAGMAFVIGSMRRRITSAIEAAANAPDRRLTTIGELVRRDVVTTPPS
                     TSVADAAALMAAERVSSLLIPVHEGWAIVTDRDLRTKVVAVRAGSELVVGEIATFPAR
                     MLPAQTLAGEALLSMFADGVHHFPVTDPHGKVIGVVTDTDLMGLGRHTPFAAKSAIER
                     ARTDAEVAEAGRELPQVVTTMVDARADPIDVGRVVSLIVDALTERLLQLAIAEHGDPP
                     CAWAWLALGSAARHEQALRTDQDHALAYDPVEGQPDPDPYFAKMAEFVTAGLEAAGIP
                     RCNGDAMATHPALRRPLDGFVDMFAEWMDRADQQATVLSSIGYDFRQVSGPLDAEPAL
                     VEAVRRARARPIFVRQLSRRALDLSPPTGFFRDLVVHAKGEHAGHLDIKHGGITIVNN
                     LARVWAVRTGVAAKGTIARLSAAAVAGGMDTSVASELSEAFHFLWDVRLRHQVDQVKA
                     GVAPDDFIDPKELASFSRSGLKEAFRAITRAQRLLRSEEGLQPR"
     gene            complement(2270..>2414)
                     /locus_tag="VIX62_00610"
     CDS             complement(2270..>2414)
                     /locus_tag="VIX62_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5858769.1"
                     /translation="PLGWDLADPTPVGNTVAFLLSDMAAGITGELIHCDGGFHAMGTD
                     LSV"
BASE COUNT          371 a          856 c          814 g          373 t
ORIGIN      
        1 gtcctgcggg agctcaccag ctcgggcgtc ggtgcggacg cgctcgtggg gagcgcgcgc
       61 gatgcgggcg agcgggtgga ggtcggcgcg ctctcacgct cggtcccggt cccgctgatc
      121 gttcgcaccg agggtgtgga cggtggctcg ttcacgacct ccgatggcgt cgaagcccgg
      181 taccccgccg tccctgccgc cgcggcacct gatgacgatg ccgacgccta cggtgccggg
      241 gattccttcg cggcggggct gacgttcgcg ttggggcagg gcacatcgat catcgacgcg
      301 ttgacgctcg cagcgcggtg cggtgcggcg tgtgccgccg gcgccggtcc ctacgcgaga
      361 cagctcaccg tcgccgacat ctgaggagac ccacggccgc gcacgcacat gcctgtcgcg
      421 ggatactcac gccgtggaca tcgccgggtt cctgggccgc taccccccgt tcgacgcact
      481 gacccacgag cacctcgccg agatcgccgg ctcggtcgag atcgagcact tcgccgccgg
      541 cgtcacgatc ctgcagcagg gaggcgaacc cgcgaccgcg caatacgtga tccgcaaggg
      601 cgcggtggaa ctcctcgatg acggacagct gctcgacctg ttggtggaag gtgagctgtt
      661 cggacagttc tcgctattgg cccacgagag cccgaccctc accgttcgtg cccaggaaga
      721 cacgctctgt tacctggtgc cagcggaggt cgcggacgcc gtcctcggat cgcacgccgg
      781 catggccttc gtgatcggca gcatgcgccg acgcatcacg tcggcgatcg aagccgccgc
      841 caacgcaccc gaccggcgcc tcaccacgat cggtgaactc gtgcgccgcg acgtggtcac
      901 gacgccgccg agcacctcgg tcgccgatgc agccgcgctc atggccgccg aacgcgtgtc
      961 gtcgttgttg atccccgtgc atgagggatg ggcgatcgtg accgaccgcg acctgcgcac
     1021 gaaggtcgtg gcggtccgtg ccggctccga gctggtggtc ggcgagatcg ccacgttccc
     1081 ggcacggatg ctgcctgccc agaccctcgc cggtgaagcg ctactgtcga tgttcgccga
     1141 cggtgtgcac cacttccccg tcaccgatcc ccacgggaag gtcatcggcg tggtcaccga
     1201 caccgacctg atgggcctcg gtcgccacac cccgttcgcg gccaagagcg cgatcgaacg
     1261 ggcgcgcacc gacgctgagg ttgccgaagc gggacgcgaa ctgccccagg tcgtcacgac
     1321 catggtcgat gcgcgcgctg atcccatcga tgtgggccgg gtcgtctcgc tcatcgtcga
     1381 cgccctgacg gagcgactgc tgcaactcgc gatcgccgaa cacggtgacc ccccgtgtgc
     1441 atgggcgtgg ctcgccttgg gcagtgccgc gcgccacgag caagcgttgc gcaccgatca
     1501 ggatcacgcc ctcgcctacg acccggtcga gggtcagccc gaccccgacc cctacttcgc
     1561 gaagatggcg gagttcgtga ccgcggggct cgaggcggcg ggcatcccgc ggtgcaacgg
     1621 cgacgccatg gcaacccacc cagccctgcg ccgaccgctc gacgggttcg tagacatgtt
     1681 cgccgagtgg atggatcgcg ccgaccaaca ggccacggtg ctctcctcga tcgggtacga
     1741 cttccgccag gtgtcgggtc cgctcgatgc cgaacccgcc ttggtggaag cggtccgccg
     1801 tgcccgcgcg cgcccgatct tcgtgcgaca gctttcgcgt cgagcgctcg acctgtcgcc
     1861 gccgaccggg ttcttccgcg acctggtggt gcacgcgaag ggcgaacacg cgggccacct
     1921 cgatatcaag catgggggca tcacgatcgt gaacaacctc gctcgcgtgt gggccgttcg
     1981 cacgggcgtc gccgccaaag gcacgatcgc ccgactgagt gccgccgcgg tagccggagg
     2041 catggacacg tcggtggcga gcgaactgtc cgaggccttc cacttcctat gggacgttcg
     2101 cctccgccac caggtcgatc aggtgaaggc aggcgtggcg ccggatgact tcatcgatcc
     2161 caaggaactc gcatccttct cccgcagcgg gctgaaggag gcgttccgcg cgatcacgcg
     2221 tgcccagcga ctcctacgca gcgaagaagg cctgcaacca cgctgatcgc tagaccgaca
     2281 ggtcggtccc catggcgtgg aacccgccgt cgcagtggat cagctcgccg gtgatgccgg
     2341 ccgccatgtc ggacaggagg aacgccaccg tgttgcccac cggcgtgggg tcggcgagat
     2401 cccatccaag cggc
//
LOCUS       DATDBZ010000023         4834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2052316, whole genome shotgun sequence.
ACCESSION   DATDBZ010000023 DATDBZ010000000
VERSION     DATDBZ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052316"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="VIX62_00615"
     CDS             complement(<1..90)
                     /locus_tag="VIX62_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HEY5858770.1"
                     /translation="MLWIIAAVLVVVGIVSIVRGALLWGILLII"
     gene            complement(250..471)
                     /locus_tag="VIX62_00620"
     CDS             complement(250..471)
                     /locus_tag="VIX62_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858771.1"
                     /translation="MPAEEDPTELAGADAAHAASIETAEAIEQAAKVNDDPEVAEVLD
                     DAAVKADQASSRVGWLRGLLHRRFGTRRA"
     gene            complement(483..1490)
                     /locus_tag="VIX62_00625"
     CDS             complement(483..1490)
                     /locus_tag="VIX62_00625"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEY5858772.1"
                     /translation="MAMVPGAMFRTIDESHPGSKLAAVFSTRAQAYAAWYLQDGDEAR
                     PSVAEGVDALTVHMPELLPIYEAMVREVGGDDPVFARMFTMWKPPGAAIACSQAVLQE
                     GERGPRLVRNYDYPAELMDAVILRSAFTGAGVIGVTDAVWGLCDGMNDRGLAVSLTFG
                     GRASMGEGFGVPIVIRYLLETCETVDDARAVLARLPIAHTHNLTIVDAGGGVITAYLN
                     PDRPPSFRRLPIAVNHQWAVESWDPVLAESTLQREWWLLRLLDDPDVDRERFLGAFLV
                     PPLYSLAHDVGRGTVYTAAYDPAEGDITYAWPTFRLPLRFDSFAEGEHEVVFPPSPPS
                     L"
     gene            1582..2712
                     /locus_tag="VIX62_00630"
     CDS             1582..2712
                     /locus_tag="VIX62_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY5858773.1"
                     /translation="MAHHTSDHARSPQAPNAARVEPSHVRSVDEAVGEVRVEVRTKRR
                     AIVLRLVFLIITVIALYILWPSLLTVFETWPELLDLNPAWFVAMLALEALSFFCIWGL
                     QRIALVTDRWFGVATAQLAANAFSRVVPGGAAAGGALQWRMLTDSGVDGARVATSLTA
                     ASLISTGTLFMLPLLTVPAALLGRPVPGGLAQAAWLGGVVFVAAFVFGWRLLFHDRLL
                     RRIGRGAQWLRNRFQRGKPPVTDLPARLVVQRDQIRDALGDTWWKALLFSLGNWLFDY
                     LALLAAITAVGSRPRPTLVLLAYSASMVLAMIPITPGGLGFVEAGLTGLLTLAGVPAG
                     DAVLAVLAYRLVSFWLPLPAGGVAAYLHRRHYRGREAVAGPG"
     gene            complement(2912..3469)
                     /locus_tag="VIX62_00635"
     CDS             complement(2912..3469)
                     /locus_tag="VIX62_00635"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY5858774.1"
                     /translation="MTAADPLPIDLGEGAVVRRYVMDDLDAIWAVAAGERERLGEWMP
                     WVEGTTSVDVQRAWLEKVVGESAGLEGCGIFVDGELAGGVGLTWDGYGIAGEIGYWTR
                     APFEGRGLITRSAQEFTRLAFEHVGLNRVVIRAGVGNVRSRAVPERLGYVEESVERGG
                     GRGLGGYYDIVVYSMLAEEWRDRPR"
     gene            complement(3466..>4834)
                     /locus_tag="VIX62_00640"
     CDS             complement(3466..>4834)
                     /locus_tag="VIX62_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY5858775.1"
                     /translation="DSGAKVMIVLDQIYHRFGQIRDDAGVQEVIAVKLNHYMKPPIKW
                     LAPLKFKSDARKLGTPLPFIPTDHRVRWWADVMGQAGPVPPEAVVEDPASEVAALVYT
                     GGTTGLSKGAMLSHSNLVSNALQASAWLNVVRQGEDGIVAALPFFHSFGTLVMDFSMT
                     KAAKLILLPRFEIDMALKAISKEKPTLFPGVPRMYIALNQDPRTPKHDLASLKACISG
                     AAPLPMAVAKRFEEITGGATVVEGYGLSECSPVTHANPLVGERKEGFIGMPLPDTDVK
                     LVDLDEPDREVPQGERGEMCIKGPQVMLGYWNRPDESGLVIRNGWLHTGDVAIMDEQG
                     YFKIVDRIKDMILVSGFNVYPTEVEAVLFHHPKILKCAVVGVPDDTTGERVKAFIVLK
                     PGETATADEIAQWCRDPDQGLTGYRVPKEVEFREELPETLIGKVLRRVLQDEERKKRE
                     AAGST"
BASE COUNT          741 a         1724 c         1583 g          786 t
ORIGIN      
        1 gatgatcagc aggatgcccc agagcaatgc cccacgaacg atggacacga tgcctaccac
       61 cacgagcaca gcggcgatga tccacaggat cgtgtccgtg gcaccctcat tccccccgtt
      121 ctgggcgagc agcgcaagac cagtctgcaa catgattccc tccctcagcg cgcacggtgc
      181 gcggtcatgg atgtcgggag gtgtcttgtc catccatgcc ggtcggcaaa cctcgccgag
      241 ggtgtcgagt caggcccgac gggtgccgaa gcgtcggtgc aagagcccgc gcagccagcc
      301 gacacggctc gacgcctgat cggccttcac ggcggcatcg tcaagcacct cggcgacttc
      361 ggggtcgtcg ttcaccttcg cggcctgttc gatcgcctct gcggtctcga tcgaggccgc
      421 atgcgcggcg tcggctccgg cgagctccgt ggggtcttct tcggctggca tcgacaccca
      481 acctacaacg acggtgggga tggcgggaac acgacctcgt gctcgccttc ggcgaacgag
      541 tcgaaccgca gagggagcct gaacgtgggc caggcgtacg tgatgtcgcc ctccgccggg
      601 tcgtacgcgg ccgtgtacac ggtgccccgg ccgacgtcgt gagcgaggct gtacagcggc
      661 ggcacgagga acgcaccgag gaaccgttcg cggtcgacat cgggatcgtc gagcagtcgc
      721 agcagccacc actcccgctg cagcgtgctc tctgccaaca ccggatccca cgactccacc
      781 gcccactggt ggttcacggc gatcggcaat cgccggaacg acggcggacg atcggggttc
      841 aggtaggcgg tgatcacgcc tccccccgcg tcgacgatcg tgaggttgtg agtgtgggcg
      901 atcggcaagc gcgcgagcac ggcgcgcgcg tcgtcgaccg tctcgcacgt ctcgagcagg
      961 tagcggatca cgatcggcac gccgaaccct tcgcccatcg acgcccgccc accgaacgtg
     1021 agtgagacgg ccaacccgcg atcgttcatc ccgtcacaga gcccccagac cgcgtcggtc
     1081 accccgatca caccggcgcc cgtgaaggca ctccgcagga tcacggcgtc catcagctcg
     1141 gcggggtagt cgtagttgcg cacgaggcgc ggaccacgct caccctcctg cagcacggcc
     1201 tgcgaacagg cgatcgccgc ccccggaggc ttccacatcg tgaacatgcg ggcgaagacg
     1261 ggatcgtcgc cgccgacctc acgcaccatc gcctcgtaga tcggcagcag ctcgggcatg
     1321 tgcaccgtga gcgcatcgac accttcggcc accgagggac gggcctcgtc gccgtcctgc
     1381 aggtaccacg cggcgtacgc ctgcgccctc gtcgagaaca ccgccgcgag cttcgagccg
     1441 gggtgcgact cgtcgatcgt tcggaacatg gctccgggta ccatcgccat cggcggttcc
     1501 tcctaccggc tcgtctcacg tacgctcctg caaggctacg cgtctgcgac gccgagacga
     1561 cgaccaggca cgaggcttcg gatggcacac cacaccagcg accatgcacg ctcgccgcag
     1621 gcgcccaacg ccgcgcgcgt ggaaccctcc cacgtgcggt cggtcgacga ggccgtcggc
     1681 gaggtccggg tcgaggtccg gacgaagcgc agggcgatcg tcctgcgtct ggtgttcctg
     1741 atcatcacgg tcatcgcgct ctacatcctg tggcccagcc tgctcacggt cttcgagacg
     1801 tggccggagc tcctcgacct gaacccggcc tggttcgtcg cgatgctcgc gttggaagcc
     1861 ctgagcttct tctgcatctg ggggctgcaa cgcatcgcgc tcgtcaccga ccgctggttc
     1921 ggggtggcga cggcgcagct cgcggcgaac gcgttcagtc gggtggtgcc gggcggagcc
     1981 gccgccggcg gagcgctgca gtggcgcatg ctcaccgact caggcgtcga cggcgcgcgt
     2041 gtggcgacct cgctcacggc cgcgtcgttg atcagcacag gcacactgtt catgttgcct
     2101 ctgctcaccg tgccggcggc gttgctcggc cggcccgttc cgggggggct cgcccaggcc
     2161 gcgtggctgg ggggcgtcgt gttcgtcgcg gcgttcgtgt tcggctggcg cttgctgttc
     2221 cacgatcggc tattgcgccg catcgggcgc ggtgcgcagt ggctgcggaa caggttccag
     2281 cgagggaagc cgccagtgac cgacctgccg gctcgcctcg tcgtgcagcg agaccagatc
     2341 cgcgacgcgc tcggcgacac gtggtggaag gcgttgctgt tctcgctcgg caactggctc
     2401 ttcgactacc tggcgttgct ggcggcgatc acggctgtcg gatcgcggcc acgtccgacg
     2461 ctcgtgctgc tcgcgtactc ggcgtcgatg gtgctggcga tgatcccgat cacgccgggc
     2521 ggcctcggat tcgtggaggc gggcctcacc ggactgctga cgctcgcggg cgtgccggcc
     2581 ggtgacgcgg tgctggcggt cctcgcctat cggctcgtgt cgttctggtt gccgttgccg
     2641 gcggggggtg tggcggccta cctgcaccga cgtcactatc gagggcggga ggcagtcgcg
     2701 ggccccggct gaagcggtca ccgtccgagc gcgtcggaac ggcagctctg gcgttgctgc
     2761 cagccactag gcaaggacct ccgtagtggc acctgcgatc cactacggag gtccttgacg
     2821 agactcgccg acgaaggcac atcctcggct accccggcac gagctcgagc acctcgcgca
     2881 ggtcggcgat cgtggccgaa caggtcgaag atcaccgcgg gcggtcccgc cactcttccg
     2941 ccagcatcga gtacacgacg atgtcgtagt agccgccgag cccccggccg ccgccgcgct
     3001 ccacgctctc ctcgacgtag ccgagccgct cggggacagc acggctgcgc acgttcccca
     3061 cgcccgcccg gatcacgacc ctgttgagtc cgacgtgctc gaaggcgagg cgggtgaact
     3121 cctgagcgga gcgcgtgatc aaccctcgcc cctcgaacgg cgcccgggtc cagtagccga
     3181 tctcacccgc gatgccgtac ccgtcccacg tcagcccgac cccgccggcg agctcgccgt
     3241 cgacgaagat gccgcagcct tccagccccg cggactcgcc gacgaccttc tcgagccacg
     3301 cccgctgcac gtcgaccgag gtcgtcccct cgacccaggg catccactcc ccgaggcgct
     3361 cgcgctcgcc ggcagcgacc gcccagatgg catcgagatc gtccatcacg taccggcgca
     3421 cgacggcgcc ctcaccaagg tcgatcggca gcggatcggc ggcggtcacg tcgagccggc
     3481 cgcttcccgc ttcttcctct cctcgtcctg cagcacgcgg cgcagcacct tgccgatcag
     3541 cgtctcgggc agctcctcgc ggaactcgac ctccttcggc acgcggtagc cggtgagacc
     3601 ctggtcggga tcgcggcacc actgcgcgat ctcgtcggcg gtcgcggtct cgcccggctt
     3661 cagcacgatg aacgccttca cgcgctcgcc cgtcgtgtcg tcgggcacgc ccacgaccgc
     3721 gcatttcagg atcttcggat gatggaagag cacggcctcc acttcggtgg ggtacacgtt
     3781 gaaacccgac accaggatca tgtccttgat gcgatcgacg atcttgaagt agccctgctc
     3841 gtccatgatc gcgacgtcgc cggtgtgcag ccatccgttg cggatcacca agccgctctc
     3901 gtcagggcgg ttccagtagc cgagcatcac ctgggggcct ttgatgcaca tctcccctcg
     3961 ctcaccctgg ggcacctcgc gatcgggctc gtcgaggtcg acgagcttca catcggtgtc
     4021 gggcaagggc atgccgatga agccctcttt gcgctcgccg accaaggggt tggcgtgcgt
     4081 gaccggggaa cactcgctca agccgtagcc ctcgacgacc gtcgcgccgc ccgtgatctc
     4141 ctcgaaccgc ttcgccaccg ccatcggcag cggggccgcg cccgagatgc acgccttcag
     4201 cgacgcgagg tcgtgcttgg gcgtgcgcgg gtcctggttc agggcgatgt acatgcgcgg
     4261 cacgccgggg aacaacgtcg gcttctcctt cgagatcgcc ttcagcgcca tatcgatctc
     4321 gaaacggggc agcaggatca gcttcgcggc cttcgtcatc gagaagtcca tcacgagcgt
     4381 gccgaaggag tggaagaagg gcagcgccgc cacgatgccg tcctcgccct ggcgcacgac
     4441 gttgagccag gccgacgcct gcagggcgtt cgagacgagg ttcgagtgtg acagcatcgc
     4501 ccccttcgac aggcccgtcg taccgcccgt gtagaccagg gccgccacct cggaggccgg
     4561 gtcctcgacg accgcttcgg gcggcaccgg accagcctga cccatcacat cggcccacca
     4621 ccgaacccgg tggtcggtcg ggatgaacgg cagcggcgtg ccgagcttgc gagcgtccga
     4681 cttgaacttc aggggcgcga gccacttgat cggcggcttc atgtagtggt tcagcttcac
     4741 cgcgatcact tcctggacgc cggcgtcgtc gcggatctga ccgaaccggt ggtagatctg
     4801 gtcgagcacg atcatcacct tcgcgcccga atcc
//
LOCUS       DATDBZ010000024         4493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_211253, whole genome shotgun sequence.
ACCESSION   DATDBZ010000024 DATDBZ010000000
VERSION     DATDBZ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211253"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..946
                     /locus_tag="VIX62_00645"
     CDS             77..946
                     /locus_tag="VIX62_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY5858776.1"
                     /translation="MLPSSFEYHRPATLGEALDVLASLGDEAKVLAGGQSLIPMMKLR
                     FASPGHLVDINRLEGLDTIEERDGDLVLGALVRHNQLAGSELIRSKYPMIAAAAPQIA
                     DPLVRNLGTIGGSLTHCDPSGDLGAVMLAANARVVLKSKDAEREVAITDFLADTFQSS
                     IEPDEILTHVKATSPSGSYGGTYLKLERKVGDYATVAVATRLVMDGGTIKTAGIGLTS
                     VGLTNIKATAAEGSLVGQAPTEEAFAEAGRLAAAASSPVDDVRGSETYKRHMVEVYVR
                     RGLARSAEIAGAA"
     gene            975..1463
                     /locus_tag="VIX62_00650"
     CDS             975..1463
                     /locus_tag="VIX62_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5858777.1"
                     /translation="MEVTITVNGTAETLDIEPRQLLVHVLREDLRLTGTHIGCDSTSC
                     GACTVLVDGTPIKSCTMFGVQVDGQEVTTVEGLLSAAGLDPIQASFKEEHGLQCGYCT
                     PGMMLVGKALIEQNPTPSDDDVRWAISGNICRCTGYMNIVKAIQAAAKTNAAATLTPA
                     DV"
     gene            1494..3860
                     /locus_tag="VIX62_00655"
     CDS             1494..3860
                     /locus_tag="VIX62_00655"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEY5858778.1"
                     /translation="MSTESEEIRGIGHSVKRVEDDRFIRGRGNYIDDITMPRMLHMAI
                     LRSPYAHAKLNGVDASRAQALPGVVAVVTGELMAQHNLAWMPTLSGDTQAVLVTDTVR
                     FQGQEVAAVIAEDPYVAKDALELIDVDYDPLDPVVTPQRALEEDAPTIRTDKEGQEGN
                     HIYHWEAGDKEATDRAFADAEVVVSLDTFYPRCHPAPLETCGCVADVDPATGQVTIYM
                     TSQAPHAIRTVFALVTGLPEEKIRIVSPDIGGGFGNKVPVYPGYVVATAASLLIGRPV
                     KWIEDRTENLISTGFARDFHMTGELALKRDGTMLGLRSSLLADEGAFHADAQPSKFKV
                     GLFHIVSGSYDLPAVHVSADGAYTNKAPGGVAYRCSFRVTEASYMIERLVSNAAYELD
                     MDPAELRRKNFVQPDQFPYESATGFVYDSGDYETAMNLALEQIGYSELRREQEEARAD
                     GKLYGIGLASFTEVVGAGPAKDYDIIGIKMNDGAELRIHPTGTAILKISSKTQGQGHE
                     TTFAQIVAEEIGIQPEKIKVMHGDTDNTPYGLGTYASRSTPTVGAATAMVTRRVREKA
                     TKLAAHLLEVSEEDIEFHAGKFTVRGAPDRIKTIQEVAFAAYTDFPDGMEAGLEAVYY
                     YNPPNLTFPFGTYAVAVEVDEDTGVWKVRRMVAVDDCGVRINPMIVEGQIQGGLTEGY
                     GIAAMELITFDENGNCIGSNFMDYLLPTAWETPKFELHETVTPSPHHPIGAKGVGESA
                     TVGSPAAYVNAVIDALQPLGVKNIDMPLTSAKVWDAIQTAKAGGAGGS"
     gene            3916..>4493
                     /locus_tag="VIX62_00660"
     CDS             3916..>4493
                     /locus_tag="VIX62_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY5858779.1"
                     /translation="MKPALIRLAADLSAKGEPYVLATVVWRRAPSSGKEGATALITPD
                     RAVRGWISGACAEPTVVREALKAIEEGTPRLLFLGPADELDLHRRDGVVAVPIACQSE
                     GALEVYVEPVLPQPQLVAIGRSPAAQALTALASGLGWRTVAVDDGGDASVREDVDRVL
                     TSLDLDAAAIDERTYVIVATQGHYDEDALERAL"
BASE COUNT          816 a         1523 c         1544 g          610 t
ORIGIN      
        1 acccgtgcga taagtggatg ctgccaccgg atgacggcga cagccgagag agccaaccga
       61 acgaggaggg tccacggtgt tgccttccag tttcgagtat caccggccgg ccacgctggg
      121 agaggcgctc gatgtgctcg cctcactcgg cgacgaggcg aaggtcctcg ccggcggaca
      181 gagcctgatc cccatgatga agctgcggtt cgcgtcgccg gggcatctgg tcgacatcaa
      241 ccgcctcgag ggcctcgaca cgatcgagga gcgcgacggc gatctggtgc tcggggcgct
      301 ggttcgacac aaccaactcg ccgggtccga gctgatccgc tcgaagtacc cgatgatcgc
      361 cgcggccgct ccgcagatcg ccgacccgtt ggtgcgcaac ctgggcacga tcggcggttc
      421 cctcacccac tgcgatccca gcggggacct cggcgcggtg atgctcgcgg cgaacgcccg
      481 tgtggtcctg aagtccaagg acgccgagcg tgaggtggcc atcaccgact tcctggccga
      541 cacgttccag tcctcgatcg agccggacga gatcctcacc cacgtcaagg cgacgtcgcc
      601 ctccggcagc tacggcggca cctacctgaa gctggagcgc aaggtgggcg actacgcgac
      661 cgtcgcggtg gccacgcggc tggtgatgga cggcggcacg atcaagaccg ccggcatcgg
      721 cctcaccagc gtcgggctca ccaacatcaa ggcgaccgcc gccgagggat ccctggtggg
      781 gcaggccccg accgaggaag ccttcgcaga agccgggcga ctggcggccg cggcctcgag
      841 cccagtcgac gacgtgcggg gcagcgagac gtacaagcgg cacatggtcg aggtctacgt
      901 gcggcgcgga ctggcgcggt ccgccgagat cgccggcgcg gcctgatccc agggaaccgg
      961 agaggacaca cgagatggaa gtcacgatca ccgtcaacgg caccgccgag accctggaca
     1021 tcgaaccacg gcagctcctc gtccacgtcc tgcgagagga cctgcgcctg accggcaccc
     1081 acatcgggtg cgactccacc agctgcggcg cctgcaccgt gctggtcgat ggcaccccga
     1141 tcaagtcgtg cacgatgttc ggggtgcagg tggacggcca agaggtcacc accgtggagg
     1201 gcctgctcag cgccgccggc ctcgacccca tccaggcctc cttcaaggag gagcacgggt
     1261 tgcagtgcgg ctactgcacc ccgggcatga tgctggtcgg caaggccctg atcgagcaga
     1321 accccacgcc cagcgacgac gacgtccggt gggcgatctc gggcaacatc tgccgctgca
     1381 ccggctacat gaacatcgtc aaggcgatcc aggccgcggc caagaccaac gccgccgcga
     1441 cgctgacccc cgccgacgtc tgatcccaga aggctgcgag aaagagagcg acgatgagca
     1501 ccgagagcga agagatccgc gggatcggcc acagcgtcaa gcgggtcgag gacgaccggt
     1561 tcatccgagg ccggggcaac tacatcgatg acatcacgat gccgcgcatg ttgcacatgg
     1621 cgatcctgcg cagcccgtac gcccacgcca agctgaacgg cgtcgacgcg agccgcgcgc
     1681 aggcgttgcc cggcgtggtg gccgtcgtca ccggggagct catggcccag cacaacctgg
     1741 cgtggatgcc caccctctcg ggtgacacgc aagcggtgtt ggtcaccgac acggttcgct
     1801 tccagggcca ggaggtcgcg gcggtgatcg ccgaggatcc ctacgtcgcc aaggatgcgc
     1861 tggagctgat cgacgtcgac tacgacccgc tggacccggt ggtcacgccg cagcgggcgc
     1921 tcgaggaaga cgcacccacg atccgcaccg acaaggaggg ccaggagggc aaccacatct
     1981 accactggga ggccggcgac aaggaggcca ccgatcgcgc gttcgccgac gccgaggtgg
     2041 tggtgtcgct cgacaccttc tacccgcgct gccaccccgc cccgctggag acgtgtgggt
     2101 gcgtcgccga cgtcgatccc gctaccggcc aggtgacgat ctacatgacc tcgcaggctc
     2161 cgcacgcgat ccggaccgtg ttcgcgctcg tgacaggact gcccgaagag aagatccgca
     2221 tcgtaagtcc cgacatcggc ggcggcttcg gcaacaaggt gccggtctac cccgggtacg
     2281 tggtcgccac ggcggcctcg ctgttgatcg ggcgcccggt gaagtggatc gaggaccgca
     2341 ccgagaacct gatctccacg ggcttcgcgc gcgacttcca catgacgggc gaactcgcgc
     2401 tcaaacgcga cggcacgatg ctgggcctgc gctccagcct gctcgccgat gagggcgcgt
     2461 tccacgccga cgcgcagccg tcgaagttca aggtggggct gttccacatc gtgagcggca
     2521 gctacgacct gccggcggtg cacgtgagcg cggacggcgc gtacacgaac aaagcgcccg
     2581 gcggcgtcgc ctaccggtgc tcgttccgcg tcaccgaggc ctcgtacatg atcgagcgct
     2641 tggtctccaa tgcggcgtat gaactcgaca tggacccggc ggaactgcgc cggaagaact
     2701 tcgtgcagcc cgaccagttc ccctacgagt cggcgaccgg gttcgtctac gactccggtg
     2761 actacgagac cgcgatgaac ctggcactgg agcagatcgg ctacagcgag ctgcgccggg
     2821 aacaggagga ggcacgcgcc gacggcaagc tgtacggcat cggactggcc tcgttcaccg
     2881 aggtggtggg ggccgggccg gcgaaggact acgacatcat cggcatcaag atgaacgacg
     2941 gcgccgagct ccgcatccat ccgacgggca cggcgatcct gaagatcagc tccaagacgc
     3001 aggggcaggg gcacgagacc acgttcgccc agatcgtggc cgaggagatc gggatccagc
     3061 ccgagaagat caaggtgatg cacggcgaca cggacaacac gccgtacgga ctgggcacct
     3121 acgcgtcgcg ctccacaccg accgtgggcg ccgccaccgc gatggtaacg cgtcgagtgc
     3181 gcgagaaggc gacgaagctc gccgcccacc tgctcgaggt ctccgaagaa gacatcgagt
     3241 tccacgccgg caagttcacc gtgcgtggcg cgcccgaccg catcaagacg atccaggagg
     3301 tggcgttcgc cgcctacacc gacttccccg atggcatgga ggccggactg gaggccgtct
     3361 attactacaa cccgccgaac ctgacgttcc cgttcgggac gtacgcggtg gccgtcgagg
     3421 tcgacgagga caccggcgtc tggaaggtac ggcgcatggt ggccgtggac gactgcggcg
     3481 tgcgcatcaa cccgatgatc gtcgaggggc agatccaggg tggcctcacc gagggatacg
     3541 gcatcgcggc gatggagctg atcacgttcg acgagaacgg gaactgcatc ggctcgaact
     3601 tcatggacta cctgttgccc acggcctggg agaccccgaa gttcgaactg cacgagacgg
     3661 tgacgccgtc gccgcatcac ccgatcggcg ccaagggcgt gggcgaatcc gcgaccgtcg
     3721 gttcacccgc cgcctacgtc aacgcggtga tcgacgccct gcagccgctg ggggtcaaga
     3781 acatcgacat gccgctcacc tcggccaagg tctgggacgc catccagacg gcgaaggcgg
     3841 ggggtgcggg gggctcatga agggaggccg aaggccgacc gtagcccccc gttcgaaggc
     3901 gggagcaggc tcggcgtgaa gccggcactg atccggctcg cggccgacct gtcggcgaag
     3961 ggcgagccgt acgtgctggc caccgtcgtg tggcggcgcg cgccgtcctc gggcaaggag
     4021 ggggccaccg ccctgatcac gcccgaccgg gccgtgcgcg gctggatcag cggcgcgtgc
     4081 gctgaaccca ccgtggtgcg cgaggcgctg aaggccatcg aggagggcac gcccaggctg
     4141 ctgttccttg gcccggccga cgaactcgac ctgcaccggc gcgacggcgt cgtcgccgta
     4201 ccgatcgcgt gccagagcga aggggcattg gaggtctacg tggaaccggt cctgccacaa
     4261 ccgcagctcg tcgcgatcgg acgttcgccc gccgcgcagg cgctcacggc gctggcctcc
     4321 gggctgggct ggcggacggt cgcggtcgat gacggcggcg atgcctcggt ccgcgaggat
     4381 gtcgaccgcg tgctgacctc gctcgacctc gatgccgccg cgatcgacga acgcacgtac
     4441 gtcatcgtgg cgacgcaggg tcactacgac gaggacgcgc tcgaacgcgc gct
//
LOCUS       DATDBZ010000025         3424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2053376, whole genome shotgun sequence.
ACCESSION   DATDBZ010000025 DATDBZ010000000
VERSION     DATDBZ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053376"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VIX62_00665"
     CDS             <1..1036
                     /locus_tag="VIX62_00665"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HEY5858780.1"
                     /translation="GHADYVEAIRAVTDRVNEAMAAPFDLEGTEFFARGSIGISVHPH
                     DAHDVETLMRNADVAMYRAKRLDPGGHAFFAADADDAQDRLSFATRLRQAVEDESWLL
                     HYQPVIDLADRRIVGAEALIRWDDVAAGIVPPGEFIPVAEELGLIEAIGDWVVDEVAR
                     QQRRWIDAGLHLEVSFNLSPRQMWTPRLGERLIGKLHDAGVDPSCMQAEITETSAMAD
                     PERTQRILRELHACGLRLALDDFGTGYSSLARLKHMPVDVLKIDREFVRHVDKDVRLA
                     GMVRAMIQVAQSLDMVPLAEGVETEGEYIFLRSNGCRLAQGFWFAHPMVARDFERLAT
                     SGEPLGPSTI"
     gene            1145..1531
                     /locus_tag="VIX62_00670"
     CDS             1145..1531
                     /locus_tag="VIX62_00670"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEY5858781.1"
                     /translation="MPRTTVEFEVTGWEPADAVEHPDGLVLGRAVVRKVFRGDLDATS
                     VADLLTVGRGDDGLAYTAIERVEGTFAGRSGTFVIVHGAADPKPPARGTIVAGAGTGD
                     LATLRGTVEIEHDDRGARFTFEYEFD"
     gene            1566..2273
                     /locus_tag="VIX62_00675"
     CDS             1566..2273
                     /locus_tag="VIX62_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5858782.1"
                     /translation="MTQRERDPSTPSHTATEGRHRMRDMDTHTRTDLITIAVGVLVLV
                     GCALLARNDLTDVEVWIFQAANELPQGYHAFVWPFMQYGTFITIPVLALIAFAFRRFR
                     LGWALLLAGTSVYLLALVAKQAVERGRPGWLLKGVETRERFGAESLGFPSGHAAVAAA
                     LTVVVAVHLSKRWAIAAVILACVVMFGRMYVGAHLPLDLVGGAALGAIAGGVANLLIP
                     PRPRQHPEGASEHHREP"
     gene            2320..>3424
                     /locus_tag="VIX62_00680"
     CDS             2320..>3424
                     /locus_tag="VIX62_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858783.1"
                     /translation="MVNRLQESSRARTIAWVVGVVSVVLIVASLIIMYLDRGVPLPED
                     VTEGDLSYGLDVLVNIFVPIIGVVLATRRPESPIGWMFLIAGFFLGLGSFTQQYALRG
                     LIVEPGSLPLVYGVAWVANWSWAIPVGVLPLLFLLFPDGGLPSERWRWVAWFDTAVVA
                     ALTAAAMLLAALEWSTPFSADPSEASGSLSAVGIATFAISFLLVMVCLPVSFVGLVTR
                     FRHSRGEERLQLKWFVTAAAFVAASFAVTGVFDGAELLSTFSLLFLYASIAIAVLKYR
                     LYDIDVVIGKAVVFAVLAAFITIVYIGLVVGVGTLVGNDRSPLLSAVAAAVVAVAFQP
                     VRERARRLANRVVYGKRATPYEVLAGFAERASET"
BASE COUNT          481 a         1169 c         1185 g          589 t
ORIGIN      
        1 cggccacgcc gactacgtcg aggcgatccg ggccgtcacc gaccgcgtga acgaggccat
       61 ggccgcgccg ttcgaccttg agggcacgga gttcttcgcg cggggctcca tcggcatcag
      121 cgtgcacccc cacgacgcgc acgacgtcga gaccctgatg cgcaacgccg acgtcgccat
      181 gtaccgcgcg aagcggctcg atcccggcgg ccacgccttc ttcgccgccg atgccgacga
      241 cgcgcaggac cggttgtcgt tcgccacccg cctccggcag gcggtcgagg acgagagctg
      301 gctgttgcac taccaaccgg tcatcgacct cgccgaccgg cgcatcgtgg gcgccgaggc
      361 gctgatccgc tgggacgacg tcgcggccgg catcgtcccg cccggagagt tcatccccgt
      421 ggcggaggaa ctcggcctga tcgaagcgat cggtgactgg gtggtggacg aggtcgcgcg
      481 tcagcaacgt cgatggatcg atgccgggct ccacctggag gtctcgttca acctgtcgcc
      541 gcggcagatg tggacgcctc gcctcggcga acggctgatc ggcaagctcc acgacgcggg
      601 ggtcgaccct tcgtgcatgc aggccgagat caccgagacc agtgcgatgg ccgaccccga
      661 acggacccag cgcatcctgc gcgagctcca cgcctgcggg ctccggctcg ccctcgacga
      721 cttcggcacc gggtactcct ccttggcccg cttgaagcac atgccggtcg acgtgttgaa
      781 gatcgaccgc gagttcgtgc ggcacgtcga caaggacgtc cgattggcgg ggatggtgcg
      841 agcgatgatc caggtggccc agagcctgga catggtgccg ctcgccgaag gcgtcgagac
      901 cgaaggcgag tacatcttcc tgcggtcgaa cggctgtcgc ctggcgcagg gtttctggtt
      961 cgcccacccg atggtggccc gtgacttcga gcggctggcc acctcgggcg aacccctcgg
     1021 cccgtccacg atctgactca acctggatcg agccggggac gccgacacct cgccttgcgt
     1081 tgagcgcggt gtccccggtg cctgggacga tggcgggcac ccaccatcca aggagcgtga
     1141 tcacgtgccc cgcaccaccg tcgagttcga ggtcaccggc tgggaacccg cagacgccgt
     1201 tgagcatcct gacgggctcg tcctgggccg cgccgtcgtc cgcaaggtgt tccgaggcga
     1261 cctcgacgcc accagcgtcg ccgacctgct caccgtgggc cgcggggacg acgggctcgc
     1321 ctacaccgcg atcgaacgcg tggaagggac cttcgcggga cgatcgggaa cgttcgtgat
     1381 cgtgcacggc gcggccgacc cgaagcctcc cgcgcgcggc acgatcgtgg cgggcgccgg
     1441 taccggcgac ctcgcgaccc ttcgcggcac ggtcgagatc gagcacgacg accgaggcgc
     1501 ccggttcacg ttcgaatacg agtttgactg agcgccgccc gccgtcgctc cctaggatgg
     1561 gcgcgatgac ccagcgggaa cgtgacccct ccaccccgag ccacacggcg accgaaggcc
     1621 gtcaccggat gcgcgatatg gacacgcaca cccgaacgga cctgatcaca atcgccgtgg
     1681 gtgtcctggt gctggtgggg tgcgccctgc tcgcacgcaa cgacctcacc gacgtcgagg
     1741 tctggatctt ccaggcggcc aacgaactcc cgcagggcta ccacgcgttc gtgtggccgt
     1801 tcatgcagta cggcacgttc atcacgatcc cggtcctggc gctgatcgcg ttcgcgttcc
     1861 ggcggttccg cctggggtgg gcgttactgc tggcgggcac gagcgtctac ctgctggccc
     1921 tggtcgcgaa acaggccgtc gaacgcgggc gacccggatg gctcctcaag ggggtcgaga
     1981 cgcgcgaacg gttcggcgcc gagagtctcg ggttcccgtc ggggcacgcg gcggtggccg
     2041 ctgcgctcac cgtggtggtg gcggtgcatc tgtcgaaacg ctgggcgatc gcggcggtga
     2101 tcttggcctg cgtggtcatg ttcgggcgga tgtacgtggg tgctcacctg ccgctggatc
     2161 tggtgggcgg cgccgcgctg ggagcgatcg cgggcggcgt ggcgaacctg ctcataccgc
     2221 cgaggccccg acagcatcct gagggagcga gcgagcacca ccgcgaaccg tgatgctgac
     2281 ccacttccgg tggcgccccg atcgggtcag gatcggctcg tggtgaaccg tctgcaggag
     2341 tcctcgagag ctcggacgat cgcgtgggtc gtgggggtgg tgtcggtcgt cctgatcgtg
     2401 gcctccctga tcatcatgta cctcgatcga ggggtcccgt tgcccgagga cgtcaccgag
     2461 ggagacctca gctacggcct cgacgtgctg gtgaacatct tcgtcccgat catcggcgtg
     2521 gtgctcgcga cgcgccgccc cgagagcccg atcgggtgga tgttcctgat cgcggggttc
     2581 ttcctcggcc tgggcagctt cacccagcag tacgccctcc gaggcctgat cgtcgaaccc
     2641 ggctccttgc cattggtcta cggcgtcgcc tgggtggcga actggtcgtg ggcgatcccg
     2701 gtgggggtct tgcccttgct gttcctgctg ttccccgacg gtggtcttcc gtcggagcgg
     2761 tggcgatggg tggcatggtt cgacaccgcc gtcgtcgccg ccctcaccgc ggcggcgatg
     2821 ctcctggcgg cgctcgagtg gagtaccccg ttctccgccg atccgagcga ggcctccggg
     2881 tccttgagcg ccgtcggcat cgccacgttc gctatcagct tcctcctggt catggtgtgc
     2941 ctgcccgtgt cgttcgtggg tctcgtgacg cggttccgac actcacgagg cgaggagcgg
     3001 ctccaactca agtggttcgt caccgcggcc gcgttcgtcg cggcatcgtt cgcggtgacc
     3061 ggcgtcttcg acggcgccga gctcctctcg accttctcgc tgctcttcct ctacgcatcg
     3121 atcgcgatcg cggtgctgaa gtaccggctg tacgacatcg atgtcgtgat cggcaaggcc
     3181 gtggtgttcg cggtgctggc ggcattcatc acgatcgtct acatcgggct cgtcgtcggc
     3241 gtcggcacgc tggtcggcaa cgatcggagt ccactgctct ccgcggtggc cgccgcggtg
     3301 gtggccgtgg ccttccaacc ggtgcgggaa cgcgcgcgac gactggccaa ccgcgtggtc
     3361 tacggcaagc gcgcgacccc gtacgaagtg ctcgcgggtt tcgccgagcg tgcgagcgag
     3421 acct
//
LOCUS       DATDBZ010000026         3704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1849564, whole genome shotgun sequence.
ACCESSION   DATDBZ010000026 DATDBZ010000000
VERSION     DATDBZ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849564"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VIX62_00685"
     CDS             <1..286
                     /locus_tag="VIX62_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858784.1"
                     /translation="PQIRDLYVPGGDIGFWQGSLRDPDDPDHAALLEAVTTRRLFEVE
                     VLYSDHEGGQRAIGRFGLAPFDDGRWLCSVVRHWNLDRPDPRPPQQQGGR"
     gene            complement(283..633)
                     /locus_tag="VIX62_00690"
     CDS             complement(283..633)
                     /locus_tag="VIX62_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY5858785.1"
                     /translation="MVTIEEVRALASTLPRSREALVHDRVKFRVGRIVYLAFSRDETQ
                     MGFGFPKEEREALVAAEPDKFLMPTTSDLRFHWVEVRLAAIDATEMRELVLDAWRMVV
                     PKRVAAEFDRLNEP"
     gene            complement(703..1083)
                     /locus_tag="VIX62_00695"
     CDS             complement(703..1083)
                     /locus_tag="VIX62_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5858786.1"
                     /translation="MSRVLVIDDERIVPIILSYALEAIGHEMIAADGGESGIRTALDE
                     HPDAIVLDLMMPEVNGIDVIDTLRHDDATREVPILVLTAVTMSREHVECMKAGADQVL
                     TKPFDPQDVADAVEDLLSPSHPAA"
     gene            1200..2111
                     /locus_tag="VIX62_00700"
     CDS             1200..2111
                     /locus_tag="VIX62_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5858787.1"
                     /translation="MAIEFLWRGEATSRELNELHAEAFETEVFSDDVWPWTTLLERHS
                     LGWVVARQDGSLVGFVNVLWDGLVHAWIQDTMVADSARHRGVATRLIEIARDGARDAG
                     CEWLHVDFDRHLRPFYFDACGFAPTDAGLIALQEEPSDDDAGAWEQQLAALWTSIDDH
                     SETAFMAQIDALVAELPEGSAIAAYERGSAFDSTGHSDLAVPLYRQALAEGLPPSRRR
                     QAVIQLASSLRNLGEVEESVALLTMERAAGSDELDDAVDAFLALSQADAGREREAVAT
                     ALTALSGHMTRYRRSLSAYAEELADRG"
     gene            2126..3409
                     /locus_tag="VIX62_00705"
     CDS             2126..3409
                     /locus_tag="VIX62_00705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5858788.1"
                     /translation="MDIDVVVIGAGFAGIAAARDLVEAGRRVVVLEARDRIGGRTWWR
                     EIPGTGVMAEYGGMFFSRATQPNLAREVERYEAAVLPGVEPDVLAWIDGDRRDEGTEA
                     IDRIQGKLASSNLGEAIRITADAFRNGGRTALDALDITAAEWVAGLDTDPEAADYVKG
                     FLVAMGGAPLEHCSVLPLLWDMVELGYSPADVFVDVGELLADGTTSLIEPMSAGVDIR
                     FGTVVSRVVHDDGGVHVTLDDGTVINAATAVVALPVNVWNDIEFDPPLAEPKRRAAAQ
                     RHPGRVSKVLAVIHDAPETYFGMGWDTPINAGFVLRPAGDGRLFMGFSVQDRVDLSDH
                     DAVAAAVNVHLPEAVVDTTAGHDWISDRFSQGTWLSVPPTWFSDGTFDALAVAEGRLA
                     FAGSDIAPEGAGWIEGAVGSGIDAAAHVQGLLSGA"
     gene            3502..>3704
                     /locus_tag="VIX62_00710"
     CDS             3502..>3704
                     /locus_tag="VIX62_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858789.1"
                     /translation="MPRRRNPRGRARSAGGILLLTVAAMALSVSAPVVAGSNETPATP
                     HERGGGPPNIVLILTDDQRVDQLS"
BASE COUNT          572 a         1274 c         1269 g          589 t
ORIGIN      
        1 ccctcagatc cgagatctct acgtccctgg gggcgacatc ggcttctggc aggggtcgct
       61 cagggatcct gacgaccccg accacgccgc tctcctcgag gccgtgacga cgcgccggct
      121 cttcgaggtg gaggtcctgt actccgatca cgagggcgga cagcgtgcga tcggtcggtt
      181 cggactcgca ccgttcgacg atggccgctg gctgtgctcg gtggtcagac actggaacct
      241 cgaccgcccc gaccctcgcc ctccgcagca gcagggcggc cgctagggct cgttcagtcg
      301 gtcgaactcg gccgccactc gcttcggcac caccatgcgc caggcgtcga ggacgagctc
      361 acgcatctcg gtcgcatcga tggccgccaa ccgaacctcg acccagtgga agcgcaggtc
      421 cgaggtggtc ggcatcagga acttatcggg ctcggccgcc accagtgcct cgcgttcctc
      481 cttcgggaac ccgaatccca tctgggtctc atcgcgcgag aacgccaggt agacgatgcg
      541 acccacccgg aacttcacgc ggtcgtgcac cagcgcttcc ctgctgcgcg gcaaggtcga
      601 cgcgagcgct cgtacttcct cgatcgtgac catggctagc cgccacgcta cgccgagcct
      661 aggacacggt gctcaccggc acgtggatcg gtcgagggca ggtcaggcag cgggatgcga
      721 gggcgacaac aggtcttcca ccgcgtcggc cacgtcctgt gggtcgaacg gcttcgtcag
      781 cacctggtcg gcgccggcct tcatgcactc gacgtgctcg cgcgacatcg tcactgcggt
      841 cagcaccagg atgggcacct cgcgggtggc gtcgtcgtgc cggagcgtgt cgatcacgtc
      901 gatcccgttg acctcgggca tcatcaggtc gagcacgatc gcgtcggggt gctcgtcgag
      961 cgccgttcgg atgcccgatt cgccgccgtc ggcggcgatc atctcgtgac cgatcgcctc
     1021 tagggcgtag gacaggatga tgggcacgat ccgttcatcg tcgatcacca acacacgaga
     1081 catgcatccc tcctcccatg tcgctcttcc ccggcatcgg cctgccgcgc ccgagactga
     1141 agccctcgcg tcgagatctc cagcgttcag ccggcaaagg cgctgctacg gtgccccgga
     1201 tggcgatcga gttcctgtgg cggggcgagg ccacgagccg tgaattgaac gaactgcacg
     1261 ccgaagcctt cgagaccgag gtcttctccg atgacgtgtg gccgtggacg acgctcctcg
     1321 agcgccacag cctcggctgg gtcgtggccc gccaggatgg ctcgctcgtc ggcttcgtga
     1381 acgtgctgtg ggacggcttg gtgcacgcct ggatccagga cacgatggtg gccgactcgg
     1441 cgcgccaccg aggcgttgcc acccggctca tcgagatcgc ccgtgacggg gcgcgggatg
     1501 cgggatgcga gtggctccac gtcgacttcg accgtcactt gcgtcccttc tacttcgacg
     1561 cgtgcgggtt cgcccccacc gacgccggcc tcatcgcgct gcaggaggaa ccgtcggacg
     1621 atgatgcggg tgcttgggag cagcagctcg cggcgctgtg gacctcgatc gacgatcaca
     1681 gcgaaacggc attcatggcc cagatcgatg cactggtcgc ggagctcccg gaaggcagtg
     1741 cgatcgcggc ctacgaacgt ggttccgcgt tcgactcgac cgggcactcc gatctcgcgg
     1801 tgccgctgta ccgacaggcg ctggccgagg gcctcccgcc cagccgccgg cgccaggcgg
     1861 tgatccagct tgcgagttcg ctccgcaacc tcggcgaggt cgaggagagc gtggcgttgc
     1921 tcacgatgga acgggcagcc ggctccgacg aactcgacga cgccgtggac gcgttcctgg
     1981 cgctgagcca ggccgatgcc ggtcgcgaac gcgaggcggt cgcgacggca ctcaccgcgc
     2041 tctcggggca catgaccagg taccgacgat cgctcagcgc ctacgccgag gagctggccg
     2101 accgagggtg atagcgtgcc tcgccatgga tatcgacgtg gtggtgatcg gggcgggatt
     2161 cgcggggatc gcggcggcgc gtgacctcgt ggaagcggga cgcagggtcg tcgtgctcga
     2221 ggcgcgtgac cgcatcggcg ggcgcacctg gtggcgcgag atccccggca cgggcgtgat
     2281 ggccgagtac ggcggcatgt tcttctcgcg cgccacccag cccaacctcg ctcgtgaggt
     2341 cgagcgctac gaagccgcgg tcctccccgg tgtagagccg gacgtgctcg cctggatcga
     2401 cggcgaccgt cgggacgagg gcaccgaggc catcgatcgc atccagggca agctcgcgtc
     2461 ctcgaacctc ggcgaggcga tccggatcac ggctgacgca ttccggaacg gcgggcggac
     2521 tgccttggat gcgctcgaca tcaccgccgc ggagtgggtg gccggcctgg atacggaccc
     2581 cgaagccgcc gactacgtga aggggttcct ggtcgccatg ggcggcgcgc cgctggaaca
     2641 ctgctccgtg cttcccctcc tgtgggacat ggtcgaactc gggtacagcc ccgcggatgt
     2701 cttcgtcgac gtgggcgaac tgctcgccga cggcacgacg agcctgatcg agcccatgtc
     2761 ggccggtgtc gacatccggt tcggcacggt ggtgtcccgg gtggtgcacg acgacggagg
     2821 tgtccacgtc acgctggacg acggcaccgt catcaacgcg gcgacggcgg tcgtcgccct
     2881 gccggtgaac gtctggaacg acatcgagtt cgacccgccg ctcgccgagc cgaagcgtcg
     2941 ggcggccgcc cagcgccacc ccggccgggt ctcgaaggtg ctggccgtga tccacgacgc
     3001 cccggagacc tacttcggca tggggtggga cacgccgatc aacgccgggt tcgtgttgcg
     3061 gccggcaggg gacggacgcc tgttcatggg cttctcggtg caggatcgcg tcgatctgtc
     3121 cgatcacgac gccgtggccg cggcggtgaa cgtgcacctg cccgaggccg tcgtcgacac
     3181 cacggccggc cacgactgga tcagcgaccg cttctcccag gggacctggc tctcggtccc
     3241 gcccacctgg ttcagcgacg gcacgttcga cgccctggcc gtggccgagg gccggctcgc
     3301 gttcgctggt tccgacatcg ccccagaggg cgccggctgg atcgagggcg cggtgggcag
     3361 tggcatcgat gccgcggccc acgtgcaagg cctgctcagc ggcgcctgag tggcgccgca
     3421 cctatcgcaa aaaacagtgc gtcgtgatag aacccccctg aagcgtcctt gagacagggc
     3481 gtaccctccg aagaaggcga catgcctaga aggcgcaacc cccgaggtcg agcacgctcc
     3541 gcgggcggga tcctgctgct caccgtggcc gcgatggcac tgtcggtgtc cgctcccgtc
     3601 gtcgccggat cgaacgagac acccgcgacg cctcacgagc gaggtggcgg accgccgaac
     3661 atcgtcctca tcctgaccga cgaccaacgg gtcgatcagc tctc
//
LOCUS       DATDBZ010000027         2579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2668278, whole genome shotgun sequence.
ACCESSION   DATDBZ010000027 DATDBZ010000000
VERSION     DATDBZ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2668278"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VIX62_00715"
     CDS             complement(<1..1144)
                     /locus_tag="VIX62_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEY5858790.1"
                     /translation="MAVTVKDIVAVPGMPLKLLAGQEQAQRPIRWVHVSELEDPTPWL
                     KGGELILTTGMGIGTTPAKQRAYVKRLAKAGLAGLGFGLGFSHDKTPRSLVTAATSED
                     FPLFEVPYPVPFIAITEAVFTRIVAEQYDTLQRAVDAEHVLTRAVMEGKGPEGIARSL
                     ADVTKGWALLLDLHGLPIASTSRAASLRAERVWDELRDSRPESTSFSVTLVDRGHHIW
                     VQPVGAQGRVEAFLAVGKPEQLSQMDRIVAGHALSLFAIELAKSRAVADAQRRLQGDF
                     FDELARGGLTNADAGRGLARFGFERDAAVLVVSLETVGEAPDPETVALAATDERSRAG
                     GGFLASAHDDGVHLLLPAEPEVDLEEVTKAIGHRVGAELRAGAGSTV"
     gene            1319..>2579
                     /gene="gabT"
                     /locus_tag="VIX62_00720"
     CDS             1319..>2579
                     /gene="gabT"
                     /locus_tag="VIX62_00720"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY5858791.1"
                     /translation="MDIPQERKLVTEIPGPKSREWFARREQAVARGVGAIHPIVTARA
                     SGSIVEDVDGNRLIDFATGIAVLNVGHTAPEVVAAIQQQAELETHTCFHVSANEPYIE
                     LAERLNALTPGDHAKKTMFANSGAEAVENAVKIARKHTGRSAIVTFDHAFHGRTLLAM
                     SLTAKVMPYKQGMGPFAPEIYRLPFAYPYRWKSEGADPETVTREALAYAVDTMHKHIG
                     EENIAAVIMEPIQGEGGFIVPSPGFVAGIAEFCATHGIVFIADEIQSAMGRAGRWYAI
                     EDEGVVPDLVTTAKSMGGGLPISAVTGRAEIMDAVHAGGLGGTYGGNPVAAAASLAVL
                     DKIEREDLLARSREVGATIMERFTAMAERYDAIGDIRGRGAMLAMEFVKPGTDEPDAE
                     LTKAIAAHALEQGVILLTCGTYGNVIRL"
BASE COUNT          434 a          908 c          866 g          371 t
ORIGIN      
        1 tcacggtgga gccggcgccg gcacggagct cagcgccgac gcgatggccg atcgccttcg
       61 tgacctcctc gagatcgacc tcgggctcgg cgggcaacaa cagatgcacc ccatcgtcat
      121 gggccgaggc gaggaacccc ccaccggccc gagaccgctc gtcggtcgcg gccagcgcca
      181 ccgtctccgg atcgggcgcc tcacccaccg tttcgaggga gacgacgagc accgcggcgt
      241 ctcgctcgaa cccgaagcgt gcgagcccgc gaccggcgtc ggcgttcgtg agtccgccgc
      301 gcgcgagttc gtcgaagaag tcgccctgga gccgccgctg ggcgtcggcc accgcacggg
      361 acttcgcgag ttcgatcgcg aacaagctca gcgcatgccc cgccacgatc cggtccatct
      421 ggctgagctg ctcgggtttg cccacggcga ggaacgcctc gacgcggccc tgcgcgccga
      481 cgggctggac ccagatgtga tggccacgat cgaccagggt cacgctgaac gaggtgctct
      541 cggggcggct gtcacgcagc tcgtcccaca cgcgctcggc ccggagcgag gccgcgcggc
      601 tggtgctggc gatcggcagg ccgtggaggt cgagcagcaa cgcccacccc ttcgtgacgt
      661 cggcgagcga ccgcgcgatg ccttcgggcc ccttgccctc catgacggca cgcgtgagca
      721 cgtgttcggc atcgacggcc cgctgcagcg tgtcgtactg ctcggccacg atgcgcgtga
      781 acacggcctc ggtgatcgcg atgaacggca ccgggtaggg cacctcgaag agcgggaagt
      841 cctccgaggt ggcggcggtc accagcgatc ggggcgtctt gtcgtggctg aaccccagcc
      901 cgaacccgag cccggccagg cccgccttcg cgagtcgctt cacgtaggcg cgctgcttgg
      961 ccggcgtggt gccgatgccc atgccggtcg tgaggatcag ctcgccgcct ttgagccacg
     1021 gcgtggggtc ctccagctcg gacacgtgca cccatcggat cgggcgctga gcctgctcct
     1081 ggccggcgag cagcttcagc ggcatgcccg gcacggcgac gatgtccttg acggtgacgg
     1141 ccatgtatcg attgtacgga acgtatagcc aggacacctc ggacgaacgg ctgcgccgct
     1201 taggatggac gaaccgtaca accccgagcg cgaaggctag ccccgccatc gcctgacagg
     1261 accccgcgtc gggcccgaca ctcgagcggt acccccagca ggagggaaac gctcgatcat
     1321 ggacatcccc caggaacgca aactcgtcac cgagatcccc ggcccgaagt cccgtgagtg
     1381 gttcgcgcgg cgcgagcagg ccgtcgccag gggtgtcggc gcgatccatc cgatcgtcac
     1441 cgcgcgtgca tcgggctcga tcgtcgaaga cgtcgacggc aaccgcctga tcgacttcgc
     1501 caccggcatc gccgtgctca acgtcgggca caccgcgccg gaggtcgtcg ccgcgatcca
     1561 gcagcaggcc gaactcgaaa cccacacctg cttccacgtc tccgcgaacg agccctacat
     1621 cgagttggcc gagcggctga acgccctgac gcccggtgac cacgcgaaga agacgatgtt
     1681 cgctaactcg ggtgccgaag cggtcgagaa cgccgtgaag atcgctcgca agcacaccgg
     1741 tcggtcggcg atcgtcacct tcgaccacgc gttccacgga cgcacgctgc tcgcgatgtc
     1801 cctcaccgcc aaggtgatgc cctacaagca gggcatgggg ccgttcgccc ccgagatcta
     1861 ccgattgccc ttcgcctatc cgtaccgctg gaagtccgag ggtgccgatc cggagaccgt
     1921 gacccgcgaa gcgctggcct acgcggtcga cacgatgcac aagcacatcg gcgaagagaa
     1981 catcgccgcg gtgatcatgg agcccatcca gggcgagggc ggcttcatcg tcccctcccc
     2041 cgggttcgtc gcggggatcg ccgagttctg cgccacgcac gggatcgtct tcatcgccga
     2101 cgagatccag tcggcgatgg gacgggccgg ccgctggtat gcgatcgagg acgagggggt
     2161 cgtgcccgac ctggtgacca ccgcgaagtc gatgggcggc ggattgccga tcagcgccgt
     2221 caccggtcgc gccgagatca tggacgccgt gcacgccggc ggcctgggtg gcacgtacgg
     2281 cggcaacccc gtcgcggccg ccgcatcgct cgccgtactc gacaagatcg agcgcgagga
     2341 tctgttggct cgctcgcgcg aggtgggcgc cacgatcatg gagcgtttca ccgcgatggc
     2401 cgagcgctac gacgcgatcg gcgacatccg cggccgagga gcgatgctcg cgatggagtt
     2461 cgtcaagccc ggcaccgacg agccggacgc cgaactgacg aaggcgattg ccgcacatgc
     2521 gctcgaacaa ggtgtcattc tgctgacgtg cggtacctac ggaaacgtca tccgactgc
//
LOCUS       DATDBZ010000028         4890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1850448, whole genome shotgun sequence.
ACCESSION   DATDBZ010000028 DATDBZ010000000
VERSION     DATDBZ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850448"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /gene="arsM"
                     /locus_tag="VIX62_00725"
     CDS             complement(<1..626)
                     /gene="arsM"
                     /locus_tag="VIX62_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEY5858792.1"
                     /translation="MSELREQVRDRYAAAAKAVGDGYAANDWDREAGCCDPGEGLGQE
                     LYDITQRSELPDEAVLASLGCGNPLMVADLHEGETVLDLGSGGGIDVLLSAQRVGPTG
                     KAYGLDMTAEMLSLARANAAKANATNVEFLEGYIEDIPLLDASVDVVISNCVINLSTD
                     KSKVLSEIFRVLRPGGRMGVSDVVAADDLNEEERRKRGSFVGCIAGAMT"
     gene            complement(623..994)
                     /locus_tag="VIX62_00730"
     CDS             complement(623..994)
                     /locus_tag="VIX62_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5858793.1"
                     /translation="MKLIQTIDAECCPQVLAAPLDREEADRLADALKVLADPARLQLL
                     SLIGAAPEGEACVCNLVEPMGLSQPTVSHHLKVLTDAGLLGREQRGRWVYYWLLPEPI
                     ELLRSALAGPVDSVTPRRKTG"
     gene            1084..2226
                     /locus_tag="VIX62_00735"
     CDS             1084..2226
                     /locus_tag="VIX62_00735"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5858794.1"
                     /translation="MLPRGFVPGGLLLTRASGGRASDPAPVIVVGGGQSGLAAARILR
                     EFRVPTVILEAGDRAAGSWPYYYDSLESFSPAGYSSLPGMPFPGAPDHYPSRDEVADY
                     LERYAAELEIEIQTNTTVATIRQEGRAFLVFTEDGRVLAASGIVAASGSFSNPYRPRF
                     QGEDTFTGELSHVADYRNPAPYDGQRVIVVGAGDSAAQVGNELALVATTSIASRHPIQ
                     FIPQHIGGHDVHYWFRETGFDTLPPEWLRIIINGSVVTDSVGFRQTLAEGKADRRPMF
                     VALDGRQAVWSDGEREPIDAIILATGYRPSVHYLRELGALNGDGAPLHVGGISSTHAG
                     LVYVGLEYQRSYASNTLRGVSEDARAVIEPLIAWIRDAPTKVGLEP"
     gene            2411..3346
                     /locus_tag="VIX62_00740"
     CDS             2411..3346
                     /locus_tag="VIX62_00740"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5858795.1"
                     /translation="MEGQDLIPPPPPPTDDRCYRHPDRVTGVHCTRCGRAICTECMVP
                     APVGHQCPTCVGEARREFAAGPGRRIAIANAKRVSVTNVLVAIIVGVFVIEVFQSGVG
                     GLINGPNAGTLIDLGAENPVLVASGETWRMVTAMFLHIGLIHLAVNAYSLYILGNVLE
                     REVGRVRFLALYLLSGLFASAASYYFSPLNSVSAGASGAIFGIFGAVFAINYRRRHTA
                     QGAMAMRAMAQIIVLNVVINVVLASYLDWRAHVGGAIAGMVMGFAFAAPGRESKQRWI
                     SIAGCVAVALVIVATVVVRTDQIRTAMGFVLDTVS"
     gene            complement(3353..>4890)
                     /locus_tag="VIX62_00745"
     CDS             complement(3353..>4890)
                     /locus_tag="VIX62_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5858796.1"
                     /translation="VLPIGRRPGLTMADRFESPGQPAHPSVAVVLAVAAALVVGIVGA
                     TIWRAGDEGERGEASQGAAAAIKTPPPINSAIEGLTTFRGNASRSYYGEGPVPLHPVI
                     RWRSPADEKLCSDSIVGVDQSEWCGTGWTGQPNVIQQPDGAIEVREGAYDANYHFVDG
                     LTGDPIGTPYVTRDLAKGSASSDPDGFPLYYAGSRDGHFRVIAMDRPEPTLLWDIDAD
                     TTVPLPLRNNDWDGAALIVGDYLLEGGENGWLYVVHLNRGYDEQGLVTVDPEIVATIA
                     GFDDELLGNLGDREVSIENSVAYRKGVVYFANSGGLVQGWDISDLLAGGTSYSQVFRF
                     WVGDDVDASIVLDSKGFLYVAAEYQRFNERSQEIGQLMKLDPKQPDDPLVWSIDAREI
                     GFEGAGGSWSTPALYGPTVVFSTAAGRVLLVDRTSGEITHELSVGAPSIASPVVVDGV
                     LILGDCLGDLYAWDMADPGVEPPLLWTMDLEGCIESTPAVWHGWLYLGTREGYLFGIA
                     DPA"
BASE COUNT          785 a         1681 c         1598 g          826 t
ORIGIN      
        1 gtcatcgcac ccgcgatgca tccgacgaag ctgcctcgtt tgcggcgttc ctcctcgttg
       61 agatcgtccg cagccacgac gtcactgacc cccatgcgac ctcccggccg gagtacgcgg
      121 aagatctcgg agagcacctt cgatttgtcg gtcgagagat tgatcacgca gttggagatc
      181 acgacatcga ccgaggcgtc gaggagcggg atgtcttcga tgtagccctc gaggaactcc
      241 acgttcgtcg cgttcgcttt cgcggcattg gcgcgggcca acgagagcat ctcggcggtc
      301 atgtcgagcc cgtatgcctt gccggtcggt ccgacgcgct gagccgagag cagcacgtcg
      361 atgccaccgc ccgatccgag atcgagcacc gtctcccctt catgaaggtc ggcgaccatc
      421 agcgggttcc cacatccgag gctggccagg accgcttcgt cggggagctc gctccgctgg
      481 gtgatgtcgt agagctcctg gccaagaccc tccccgggat cgcagcagcc agcttcccgg
      541 tcccagtcgt tcgccgcgta gccgtccccg accgccttcg cggcggcggc gtagcgatcg
      601 cggacctgtt cgcggagttc gctcatcccg tcttcctccg tggtgtgacg ctgtcgaccg
      661 ggccagcgag cgccgaccgc agcagttcga tcggctcagg taggagccag tagtagaccc
      721 agcgcccgcg ctgctcccgg ccgaggagac cggcatcggt cagcaccttc agatggtgac
      781 tcacggtcgg ttgcgacagg cccatcggct cgacgaggtt gcagacgcat gcctcgccct
      841 cgggggctgc cccgatcagg ctcaggagct ggaggcgggc gggatcggcc aacaccttca
      901 acgcgtcggc gagccgatcg gcttcctctc ggtccagcgg cgcggcgagc acctgcgggc
      961 agcactccgc gtcgatcgtc tggatcagct tcatgcggag atattgacgt tcatctatgg
     1021 aactgtcaac gtatccacca ccatcgatat gaaggaggtg ttcgggatga tgctgctcga
     1081 cccatgctgc cccgagggtt cgtgcccgga gggttgctgc tgacccgagc gagcgggggc
     1141 cgggcttctg acccggctcc cgtgatcgtc gtcggcggcg ggcagtcggg cctcgcggcc
     1201 gcccggattc ttcgcgagtt ccgcgtgccg acggtgatcc tcgaggcagg cgaccgcgca
     1261 gccggatcgt ggccgtatta ctacgacagc ctggaatcat tctctccggc gggatacagc
     1321 tcgctccccg ggatgccgtt ccccggcgcg ccggatcatt acccgagccg tgatgaggtc
     1381 gccgactacc tcgagcggta cgccgccgag ctcgaaatcg agatccagac gaacacgacc
     1441 gttgcgacga tcaggcaaga aggtcgggca ttcctggtct tcaccgagga cgggcgggtt
     1501 ctggcggcct caggcatcgt ggccgcgagc ggatcattct cgaacccgta tcgcccgcgt
     1561 ttccagggtg aggacacctt cacgggtgaa ctctcgcacg tcgccgacta ccggaacccg
     1621 gcgccgtacg acggtcaacg tgtgatcgtc gtcggtgcgg gagactccgc cgctcaggtt
     1681 gggaacgaac tcgcgctcgt cgccacgact tcgatcgcca gtcgccatcc gatccagttc
     1741 atcccccagc acatcggtgg ccacgacgtc cactactggt tccgcgagac gggcttcgac
     1801 accctcccgc cggaatggct gcgcatcatc atcaacggca gcgtggttac cgacagcgtc
     1861 gggtttcgac agacgctcgc cgagggcaag gcggatcgac gcccgatgtt cgtcgcgctc
     1921 gacgggaggc aggcggtttg gagcgacggc gaacgagaac ccatcgatgc gatcatcctt
     1981 gccaccggct accgacccag cgtgcactac cttcgtgagc tcggggcgct caacggcgat
     2041 ggggcaccgc tccatgtcgg gggcatctct tcaacgcatg ccgggctcgt ctacgtcggt
     2101 ttggaatacc agcgttcgta tgcctcgaac acgctcaggg gtgtcagcga ggacgcgagg
     2161 gcagtgatcg aaccgctgat cgcgtggatc cgcgacgcgc cgacgaaggt tggccttgaa
     2221 ccctgatcat catgcatcgt gaaccgatgg ggtccgcccg acccaacccg ttgcatggtc
     2281 cagcccgacg gcggggaacg aacgtcaggc gaacaggagc gagcgaagag aaacccctga
     2341 gtcgttgcag gccacggctg gtgggctccg acctcatgca tccacgtccg cgggccgtag
     2401 catgagggac atggaaggtc aggacctgat ccccccgccg cctccgccca ccgacgatcg
     2461 gtgctaccgc catcccgatc gcgtcacggg cgtgcactgc acgcgctgcg ggcgcgcgat
     2521 ctgcacggag tgcatggtgc ctgcccctgt gggccaccaa tgccccacgt gtgtcggcga
     2581 ggctcgcagg gagttcgcgg ccgggccggg gcgtcggatc gccatcgcga acgcgaagcg
     2641 ggtctcggtc acgaacgtgc tggtggcgat catcgtcggg gtcttcgtga tcgaagtgtt
     2701 ccagagcggc gtcggcggcc tgatcaacgg ccccaacgcc ggcacgctga tcgacctcgg
     2761 ggcggagaac cccgtgctcg tcgcctccgg cgagacctgg cggatggtca ccgcgatgtt
     2821 cctgcacatc ggattgatcc acctcgcggt caacgcctac tcgctgtaca tcctcggcaa
     2881 cgtgctggaa cgcgaggtgg gccgggtgcg gttcctggcc ctctacctcc tgtccggcct
     2941 gttcgccagc gcggcgtcct attacttcag cccgctgaac tcggtgagcg ccggtgcctc
     3001 gggcgcgatc ttcggcatct tcggggcggt gttcgcgatc aactaccgcc gacgccacac
     3061 cgctcagggc gcgatggcga tgcgggcgat ggcacagatc atcgtgctca acgtggtgat
     3121 caacgtggtg ctcgccagct acctcgattg gcgagctcac gtcggcggcg cgatcgccgg
     3181 gatggtcatg gggttcgcct tcgccgcgcc gggccgggag tcgaagcagc gctggatctc
     3241 gatcgcggga tgcgtcgcgg tcgccctggt catcgtcgcg accgtggtgg tgcgcaccga
     3301 tcagatccgc accgcgatgg gttttgtcct ggacaccgtg tcctgagtcg cgctacgccg
     3361 gatcggcgat gccgaacagg tacccctccc gggtgcccag gtagagccat ccgtgccaca
     3421 cggccggcgt ggactcgatg cagccctcga ggtccatcgt ccacagcagc ggcggctcga
     3481 ctccgggatc ggccatatcc cacgcgtaga gatcgccgag gcaatccccc aggatcaaca
     3541 cgccgtccac gaccaccggc gaggcgatcg acggggcgcc caccgagagc tcatgcgtga
     3601 tctcgccgga cgtgcgatcc accaggagca cccgtccggc cgcggtcgag aacacgacgg
     3661 tcggaccgta gagggccggc gtcgaccaac tcccacccgc cccctcgaag ccgatctcgc
     3721 gcgcatcgat gctccagacc agcggatcgt ccggctgctt cggatcgagc ttcatgagct
     3781 gaccgatctc ctgggagcgc tcgttgaacc gctggtactc ggccgccacg tagaggaacc
     3841 ccttcgagtc cagcacgatc gaggcatcca cgtcgtcgcc cacccagaac cggaacacct
     3901 gactgtagga ggtgccgccc gccagcaggt cggagatgtc ccagccctgc accagcccgc
     3961 ccgagttcgc gaagtacacg acccccttac ggtaggcgac cgagttctcg atcgagacct
     4021 cacgatcgcc caggttgccg agcagctcgt cgtcgaaccc cgcgatcgtg gcgacgatct
     4081 ccgggtcgac ggtcaccagc ccctgttcgt cgtagccgcg gttcaggtgg accacataca
     4141 gccatccgtt ctcgccgcct tcgagcaggt agtcgccgac gatgagcgcg gcaccgtccc
     4201 agtcgttgtt gcggagcggc agcggcacgg tggtgtcggc gtcgatgtcc cacagcaacg
     4261 tcggctcggg gcggtccatc gcgatcaccc ggaagtgacc gtcccgggat cccgcgtagt
     4321 acagcgggaa cccgtccggg tcggagctgg ccgagccctt cgccaggtcg cgggtcacgt
     4381 acggcgtccc gatcgggtct ccggtcaggc cgtccacgaa gtggtagttc gcgtcgtagg
     4441 cgccctcgcg aacctcgatc gcgccatccg gttgctggat cacgttcggc tgacccgtcc
     4501 agccggtgcc gcaccattcg ctctgatcca cgcccacgat cgagtccgag cacagcttct
     4561 cgtcggcggg gctccgccac cggatcaccg ggtgcaacgg caccggcccc tccccgtagt
     4621 aggaacgcga cgcgttgccc cggaacgtgg tgagcccttc gatcgcactg ttgatgggcg
     4681 gcggcgtctt gatcgcggcg gcggcgccct gggatgcttc gccgcgctct ccttcatcgc
     4741 cggcgcgcca gatcgtggcg cccacgatgc ccacgacgag tgcggccgcg acggcgagca
     4801 ccacggcgac gctgggatgg gctggttgac ccggcgactc gaaccggtcg gccatggtca
     4861 gaccgggtcg ccggccgatc ggtaggaccc
//
LOCUS       DATDBZ010000029         6273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1031154, whole genome shotgun sequence.
ACCESSION   DATDBZ010000029 DATDBZ010000000
VERSION     DATDBZ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031154"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VIX62_00750"
     CDS             complement(<1..1526)
                     /locus_tag="VIX62_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5858797.1"
                     /translation="MAAPTTRVPGSFVHLDVRSCFSLKEGAFTPEQLAARAADLDMPA
                     VAMTDRDGLYGAARFVRACADEGVKPILGASITVRATQPGGRGSERDAHVVLLALDDT
                     GYANLCHLLTDAHMLGERGDPWVDATQICAHAAGLVAIAGPRSHPGRAAIAGRVDAAA
                     RLFDPFREAFGPERLFVGVEHRVERGSGDEARALLRLANRVGVRAVATNPVRYLEPGD
                     AFVADALECMRRIVPVADTNVSRRNAEGWLKPTPAMRALFDERSDLADMTLEIAEMSA
                     FDLGLDRIYFPDFPTPEGRSADALLAERTWRGVHERGMHEDEALRDRLHHELSMIRRM
                     GFAAYFLTVADIVADVKAMGIRAACRGSAAGSLVCYLTGISDVDALRHDLSFERFMNP
                     MRKDLPDIDIDVESARREDVYDMILSRHGTERAGCVAMIDTYRARSAIREVGKALGLP
                     EVELGMIAKSFPHISASHLREAIDRLPELQGINLRFEQMELLFSVAERISGFPRHIAM
                     "
     gene            complement(1541..1786)
                     /locus_tag="VIX62_00755"
     CDS             complement(1541..1786)
                     /locus_tag="VIX62_00755"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEY5858798.1"
                     /translation="MTKRFDEEITVERDTGMPCAFEWRGRRYDVIDVIGKWRIEGRWW
                     SDGRDREYWRVEARGGAVWDLYFDRGKDRWHMERLWD"
     gene            1968..3230
                     /gene="rocD"
                     /locus_tag="VIX62_00760"
     CDS             1968..3230
                     /gene="rocD"
                     /locus_tag="VIX62_00760"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEY5858799.1"
                     /translation="MTTVADPNEQLSPASATSSEAQIELMERYSAHNYHPLPVVVADG
                     EGAWVTDVDGKRYLDMLAAYSALNFGHRHPDLVAAAREQLDHVTLTSRAFHNDKLGPF
                     CKELAELCGMEMVLPMNTGAEAVETALKTARRWGYDVKGVPEDRAKILTCSGNFHGRT
                     IAIVSFSTDPDARGGFGPFTPGFETVPYGDIDALDAALADPDVVGFLVEPIQGEAGVV
                     VPPDGYLRRARALCTERNVLLLADEIQSGLGRTGRTFACEHEGVQPDVYILGKALGGG
                     IVAISAVVSTREVLGVFRPGEHGSTFGGNPLACAVGLEVMRLLRTGEFQQRADELGTA
                     LIARLRAEAPLEAVSEIRGKGLWIGIELTPEAAPAREVCERLLDMGVLAKDTHETTVR
                     LAPPLCVTQEDLDWATERILEALRERVA"
     gene            3305..3880
                     /locus_tag="VIX62_00765"
     CDS             3305..3880
                     /locus_tag="VIX62_00765"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEY5858800.1"
                     /translation="MAARPLRIVEDLPDRHRAFWFVPGTQWMNDPRHDGEVRFLDGAW
                     ELEPLVRERGVLSFAFPDSAYGILMNTDDEGRFTGYYVNIQSPLQRWDGGFDYVDWFL
                     DVRIPIDRSTYAWKDEDELAEAIERGLITSDEARGIRWAGERAIEHLLLREPPFDLDW
                     EDWRPDPAWTPPTLAERWNDAVGMDPRGSHT"
     gene            3937..4803
                     /locus_tag="VIX62_00770"
     CDS             3937..4803
                     /locus_tag="VIX62_00770"
                     /inference="COORDINATES: protein motif:HMM:NF021615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEY5858801.1"
                     /translation="MDDSGGFTTPPPPPPPGGAGGGAGGGLAPRGLGDILSAAFEIYK
                     NNASGLIMIVAIVVVPLTFISAFLGGVVFAPDTHTETIFGQTVEVSTRSATVGIFAAL
                     IGVVIAVIISAVLQAALIRGAAQASIGDAVDIDASYKWGFARFGSVLLISILVGLAVL
                     GGLILFIIPGLILIVMFAVAIPALVVENRKGTEAMGRSWNLVKGHFWHVVVVILVAAI
                     IAGVVSGIINGIGTAISDNWFVTWVFQAIGQIIVAPFSALVAVLLYVDLRARTEALTA
                     DQLRGELATNPT"
     gene            4930..5739
                     /locus_tag="VIX62_00775"
     CDS             4930..5739
                     /locus_tag="VIX62_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858802.1"
                     /translation="MPLAPLTPGSIIGRAFSTLGEHWKPLLGISAVFTAISFVAGLLI
                     GLAAGDPGDTMDPDTSATIAVLVGTGLYFIVWMAMTGALTRLVASEVAGAPGGVAESL
                     RYGLRHLGSILVVGLLLFAILFVEILVGSLFAGALHAPFLLFFVLLGALFLGLTLSMS
                     IPALVIENKRGSEALSRSWALIMSAFGHSLGTLALTFLVLVAGSIAVGVVGTAGEAMS
                     VIANLALQVITLPFVSLVLVLLYVNLRVKAGGITKDSLRAELRATGPTPMP"
     gene            complement(6050..>6273)
                     /locus_tag="VIX62_00780"
     CDS             complement(6050..>6273)
                     /locus_tag="VIX62_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858803.1"
                     /translation="MRDIIDRAAEAAGRDPSTILRASSLSISEDWDEVRTAFGWMAAE
                     GIGYLVIEWPGEGEGRVRTFLDEVLPTLG"
BASE COUNT          916 a         2168 c         2157 g         1032 t
ORIGIN      
        1 tgcatcgcga tgtggcgagg gaacccggag atccgctcgg ccaccgagaa cagcagttcc
       61 atctgctcga accgcaggtt gatcccctgg agttctggca ggcggtcgat cgcctcgcgc
      121 aggtggctcg ccgagatgtg cgggaacgac ttcgcgatca tgcccagctc gacctcgggc
      181 agaccgagcg ccttgcccac ctcgcggatc gccgagcgtg cgcggtaggt gtcgatcatc
      241 gcgacgcatc ccgcccgctc ggtgccgtga cgggacagga tcatgtcgta gacgtcttcg
      301 cgccgggcgg actcgacgtc gatgtcgatg tcgggcagat ccttgcgcat cgggttcatg
      361 aaccgttcga acgacaggtc gtgacgcagc gcgtcgacgt cggagatgcc ggtgaggtag
      421 cacacgagcg agccggcggc gctgccccgg cacgcggccc ggatgcccat agccttcacg
      481 tcggcgacga tgtcggccac ggtcaggaag taggccgcga accccatgcg ccggatcatc
      541 gagagctcgt ggtgcagccg gtctcggagc gcttcgtcct cgtgcatgcc gcgttcgtgg
      601 acgccgcgcc aggtgcgttc ggccagcagg gcatcggcgc tgcggccttc gggcgtgggg
      661 aagtcgggga agtagatccg gtccagtccc aggtcgaacg cgctcatctc ggcgatctcg
      721 agcgtcatgt cggccaggtc ggaccgttcg tcgaacagcg cacgcatcgc cggagtcggc
      781 ttcagccagc cttcggcgtt gcggcgggag acgttggtgt cggccaccgg cacgatgcga
      841 cgcatgcact cgagcgcgtc ggccacgaac gcatcgccgg gctccaggta gcgaaccggg
      901 ttggtcgcca ccgcccgcac gcccacccgg ttggccaggc gcagcagcgc gcgggcttca
      961 tcccccgatc cgcgctccac ccggtgttcc acgcccacga acaaccgctc gggaccgaac
     1021 gcctcgcgga acggatcgaa caggcgcgcg gcggcatcga cccgtcccgc gatcgcggcg
     1081 cgcccggggt gcgaccgtgg cccggcgatc gcgacgagcc cggccgcgtg cgcgcagatc
     1141 tgcgtggcat cgacccaggg gtcgccgcgc tcacccagca tgtgcgcgtc ggtgagcagg
     1201 tgacacaggt tggcgtagcc cgtgtcgtcg agagcgagca ggacgacatg ggcgtctcgt
     1261 tcgcttcccc gcccacccgg ttgcgtcgcc cggaccgtga tcgatgcgcc aaggatgggc
     1321 ttcacgcctt cgtcggcaca cgcgcggacg aaccgggcag caccgtagag cccatcgcgg
     1381 tcggtcatgg ccaccgccgg catgtccagg tcggcggcgc gcgcggccag ctgctccggc
     1441 gtgaacgcgc cctccttcaa cgagaagcac gaccgcacgt ccaggtgtac gaacgagccg
     1501 gggacgcgcg tcgtcggagc ggccactagt ccattccaga ctagtcccag agccgttcca
     1561 tgtgccagcg gtccttgccg cggtcgaagt acagatccca taccgcgccg ccgcgcgcct
     1621 cgacccgcca gtactcgcgg tcgcgcccgt ccgaccacca gcggccttcg atccgccact
     1681 tgccgatcac gtcgatgacg tcgtagcgcc tgcctcgcca ctcgaacgcg cacggcatcc
     1741 cggtgtctcg ctcgaccgtg atctcctcgt cgaaccgctt cgtcaccgtg gcctcgatgt
     1801 gatcgacctc acagacatga actcgaacat atgttcgagc ggaggatgaa tgtacgcccg
     1861 ggctccgaca tccgtcaaga cgcgccgaac gaccgtcggc gtcgaggtcg ggttcgcgaa
     1921 ccccttgcgc cgcccaacgc ttgggtcgcg gccgtatcat gctggccatg acgacggtgg
     1981 ccgatccgaa cgagcaactg tcccccgctt ccgccacgag ctccgaggcc cagatcgagc
     2041 tgatggagcg gtattcggcg cacaactatc acccgcttcc cgtggtggtg gccgacggcg
     2101 agggcgcctg ggtcaccgac gtcgatggca agcgttacct cgacatgctg gccgcgtatt
     2161 cggcgctgaa cttcgggcac cgtcatcccg acctggtggc cgccgcgcgc gagcagctcg
     2221 accacgtcac gctcacctcg cgcgcgttcc acaacgacaa gctcggtccg ttctgcaagg
     2281 agctcgccga gctctgcggc atggagatgg tgctgcccat gaacaccggg gccgaggccg
     2341 tcgagaccgc gctgaagacc gcgcgtcgat ggggctacga cgtcaagggc gtgccggagg
     2401 accgcgcgaa gatcctcacg tgcagcggca acttccacgg tcgcacgatc gcgatcgtga
     2461 gcttctcgac cgacccggac gcgcgcggtg gcttcggccc gttcacgccg gggttcgaga
     2521 ccgtgcccta cggcgacatc gacgcgctgg atgccgcgct cgccgatccc gacgtcgtgg
     2581 ggttcctggt cgaaccgatc cagggcgagg ccggggtcgt ggtgccgccc gacgggtacc
     2641 tgcggcgcgc ccgtgcgctg tgcaccgagc gcaacgtgct gctcctcgcc gacgagatcc
     2701 aatccgggct gggccgcacc ggccgcacgt tcgcgtgcga acacgagggc gtgcaacccg
     2761 acgtctacat cctgggcaag gcgctgggcg gcggcatcgt cgcgatcagc gccgtggtgt
     2821 ccacccgcga ggtgctgggc gtgttccggc ccggcgaaca cgggtcgacg ttcggcggga
     2881 acccgctggc ctgcgcggtg ggcctcgagg tcatgcggct gctgcggacc ggggagttcc
     2941 agcagcgggc cgatgagctg ggcaccgcgc tgatcgcacg gctccgcgcc gaagctccgc
     3001 tggaagccgt gagcgagatc cgcggcaagg gcctgtggat cggcatcgag ctcacccccg
     3061 aggcggcgcc ggcccgcgag gtgtgcgaac ggctcctcga catgggcgtc ctggcgaagg
     3121 acacgcacga gaccaccgtg cgcctggcgc ccccgctgtg cgtgacgcag gaggacctgg
     3181 actgggcgac ggagcggatc ctggaagcgc tgcgcgagcg agtggcctga cccccgccga
     3241 gccggaacct tccctcgagc ccggcgaggc cgtcacgctt cgcgaggtgt ggcacggtcg
     3301 cgtcgtggcg gcacgtccgc tgcggatcgt cgaagacctg cccgaccggc accgggcgtt
     3361 ctggttcgtc cccggcaccc agtggatgaa cgaccctcgc cacgacggcg aggtccgctt
     3421 cctcgatgga gcctgggagc tcgagccctt ggtgcgcgaa cgcggggtgc tgtcgttcgc
     3481 gttcccggac tcggcgtacg ggatcttgat gaacaccgat gacgagggcc ggttcaccgg
     3541 ctattacgtc aacatccagt cgccgctcca gcgatgggac ggcgggttcg actacgtgga
     3601 ctggttcctg gacgtgcgga tcccgatcga tcggtcgacc tacgcctgga aggacgagga
     3661 cgagctggcc gaggcgatcg aacgcggact gatcacctcg gacgaggcga ggggcatccg
     3721 ctgggccggg gagcgcgcca tcgaacacct cctgctccgg gaacccccgt tcgatctgga
     3781 ctgggaggat tggcgccccg atcccgcctg gaccccgccc acgctggcgg aacgctggaa
     3841 cgacgcggtg gggatggatc ctcgcggctc ccacacctga tcgggcccgg ggcgtaccat
     3901 ccgggcgttc ccctacctgt tcgaagggag ttcctcatgg acgattccgg cgggttcacg
     3961 acgccgccac cgccaccgcc tccgggcggt gcaggaggtg gcgcgggtgg tggcctggcc
     4021 ccgcgagggc tcggcgacat cctcagcgcc gccttcgaga tctacaagaa caacgcgtcc
     4081 ggcctgatca tgatcgtcgc gatcgtggtg gtgccgctga cgttcatcag cgcgttcctc
     4141 ggcggcgtgg tcttcgcgcc cgacacgcac acggagacca tcttcggcca gacggtcgag
     4201 gtgagcaccc ggagcgccac cgtcgggatc ttcgcggcgc tgatcggcgt cgtgatcgcg
     4261 gtgatcatct ccgcggtgct gcaggcggcc ctgatccgag gcgccgccca ggccagcatc
     4321 ggggatgcgg tcgacatcga tgcctcgtac aagtgggggt tcgctcgatt cggcagcgtg
     4381 ctgctgatct cgatcctggt gggcctcgcg gtcctcggcg gactgatcct gttcatcatc
     4441 cccggtctca tcctgatcgt gatgttcgcc gtggccatcc ccgccctcgt ggtcgagaac
     4501 cgcaagggca ccgaagccat ggggcgatcc tggaacctgg tgaagggaca cttctggcac
     4561 gtcgtggtgg tgatcttggt cgcggccatc atcgccgggg tggtcagcgg gatcatcaac
     4621 ggcatcggca ccgcgatcag cgacaactgg ttcgtcacct gggtcttcca ggcgatcggc
     4681 cagatcatcg tggcgccgtt ctcggcgctc gtcgcggtgc tgctgtacgt ggacctgcga
     4741 gcccgaaccg aggcgctcac cgccgatcag ctccgtgggg aactggcaac gaaccccacc
     4801 tgatctcagg gcatatccgc atggcgaagg gcgtcccgcg gggcgccctt cgcacggaga
     4861 ggtgaatcgc gaatggccgc catcccacca ccgcctccac cgccgaaccc gggcagtagc
     4921 ggtggcgcct tgcccctcgc tcccctcacc cccgggtcga tcatcggccg ggcgttctcc
     4981 accctgggcg agcactggaa gccgttgctc gggatcagcg ccgtgttcac cgcgatctcg
     5041 ttcgtcgcgg ggctgctgat cgggctggcg gcgggcgacc cgggcgacac catggatccg
     5101 gacacgtcgg ccacgatcgc ggtgctggtg gggaccgggt tgtacttcat cgtgtggatg
     5161 gccatgaccg gggccctcac ccgcctcgtg gcgtccgagg tggccggagc ccccggcggt
     5221 gtcgccgaga gcctccggta cggcctccgc cacctggggt cgatcctcgt cgtggggctg
     5281 ctgttgttcg cgatcctctt cgtcgagatc ctcgtcggct cgctgttcgc cggggcgctg
     5341 catgcaccgt tcctgctctt cttcgtgctc ctcggtgcgt tgttcctcgg gttgacgctg
     5401 tcgatgtcga tccccgcgtt ggtgatcgag aacaagcgcg ggagcgaggc attgtcccgg
     5461 tcctgggcgt tgatcatgtc ggcgttcgga cattcgctgg gaacgctggc gctgacgttc
     5521 ctcgtgctcg tcgccggctc gatcgcggtc ggcgtggtcg gaaccgcggg cgaggcgatg
     5581 tccgtgatcg cgaacctcgc gttgcaggtg atcacgttgc cgttcgtgtc cctggtgctg
     5641 gtgctgctct acgtgaacct gcgggtgaag gcgggcggca tcacgaagga ttcgctccgc
     5701 gcggagctgc gggcgacggg ccccacgccg atgccgtagc atggggcctg agccgaccgg
     5761 cctgaccgga ggctcgtggg gctgaccaga tccctggagg ccgcgaccgt gcttcgtgtc
     5821 gcggcctttc gtgcgttcga cgtgcgttcc cggccgcggg aaggggatcg atcgtgaaga
     5881 cgaccgcagt gtgcttcggc atcgccctgg tgctcggcct catcaactac gtgctcgccg
     5941 tccgtccacg gctccactcg atggaggacg tactggatga aaccggcacg ctcgagtcgt
     6001 ggtgggccat ggaggccgcc cccgaccctc aggtccctcg gccgacgact cagcccagcg
     6061 tgggcaacac ctcgtcgagg aaggtccgaa cccggccctc tccctcgccc ggccactcga
     6121 tcaccaggta accgatccct tcggccgcca tccagccgaa ggcggttcgc acctcgtccc
     6181 agtcctcgct gatcgacagg ctcgaggccc gcaggatcgt cgaggggtcc cggccggctg
     6241 cttcggcggc ccggtcgatg atgtcgcgca tct
//
LOCUS       DATDBZ010000030         7167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2669583, whole genome shotgun sequence.
ACCESSION   DATDBZ010000030 DATDBZ010000000
VERSION     DATDBZ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669583"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VIX62_00785"
     CDS             complement(<1..399)
                     /locus_tag="VIX62_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858804.1"
                     /translation="MMGPTELFVVVVVVVAIGFWISRRLQSDDEYIARWADATGVEIT
                     PEQAPYVRRYLDWSRRGRRIGALVGFVSPWLYSAISGRTFDEGAWAVATMLVGYLLGA
                     LVAEIVVNRALESGPAAVMQPRRLTDYLPGH"
     gene            complement(502..1896)
                     /gene="glnA"
                     /locus_tag="VIX62_00790"
     CDS             complement(502..1896)
                     /gene="glnA"
                     /locus_tag="VIX62_00790"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY5858805.1"
                     /translation="MAKEQGAQIVDFRFTDLPGQVQHLSVPVHELTEEKLTEGQPFDG
                     SSIRGFQEIQESDMLLMPDCDTAFMDPFTKHPTMNITCFVKDPVTGEDYSRDPRNIAK
                     KAELYLKQTGIADRSYWGPEAEFYIFDSIRYDQNEFEGYYHVDSVAGAWQTGKEETGG
                     NLGYKPRYKGGYFALPPMDKYQDLRTDMVLNLEKVGLTIEVHHHEVGTAGQAEIDMRY
                     DTLLTTADNVMKYKYVVKNTAYQAGKTVTFMPKPLFMDNGSGMHTHQSLWTGDENLFW
                     DEVGYGGISDAARWYIGGLIAHAPSLLAFTNPTTNSYRRLVPGYEAPIKLVYSQRNRS
                     ACVRIPLGSKSPAAKRLEFRTPDPSCNPYLAFSAMLMAGIDGIKNKIEPPDPIDKDLY
                     DLPPEEHAAVKQVPGSLLEVLGELEADHQWLLEGGVFTQDVIDTWIEYKRIEEHDAVA
                     LRPHPYEFSLYYDV"
     gene            2119..2250
                     /locus_tag="VIX62_00795"
     CDS             2119..2250
                     /locus_tag="VIX62_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858806.1"
                     /translation="MDERLAAHDDPQDWDLPDWLFDDQGEMIREVEDTEDDRFADAS"
     gene            complement(2650..3312)
                     /locus_tag="VIX62_00800"
     CDS             complement(2650..3312)
                     /locus_tag="VIX62_00800"
                     /inference="COORDINATES: protein motif:HMM:NF044852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5858807.1"
                     /translation="MAFLALLSDRSPTVALPALGDLLPDLKLEPLSLASLDHVVGFDP
                     TAVLVDAVENPAQAWSVLQELRGRDDRVPAMLIVERDQLERYPWHEVADEFVYPGAPE
                     GELRVRLAMLRRRSGAGDGSVIRLGALALDTETYRVTANGRSLDLTFKEFELLRFLAS
                     SPGRVFTRPTLLREVWGYDFYGGTRTVDVHVRRLRAKLGPEHEHLIETVRSVGYRAAD
                     PD"
     gene            3393..4193
                     /locus_tag="VIX62_00805"
     CDS             3393..4193
                     /locus_tag="VIX62_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5858808.1"
                     /translation="MKTVQGITSHHWRVPAYPYTVTTQDGVEIAGTRLGEPGADLPAL
                     ILSHGMIGWHRKPKFAVFAEKLTPWFAVYAMDMRGHGESTGVCDFGGLEIYDIEAVHQ
                     RARADGHERIITVGTSMGGIATIRHGALIGGVDAVVGISSLAYWDWHDDAHPKLRRNM
                     HTKIGTPAGRAALRAFGVRMPEHWDEPESPEEVIGKIAPTPVIIVHGTDDKLFPPSHA
                     QRLFDAAGEPKRLLLGEGFGHAEDGLSPAFASKLVSVVHESVGWAWSG"
     gene            4190..4420
                     /locus_tag="VIX62_00810"
     CDS             4190..4420
                     /locus_tag="VIX62_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5858809.1"
                     /translation="MRLFAALREQADASRVEASGRTVGEVVDQLSERYGERFASIAAV
                     GSYVVNGERASRDTVIAEGDEVALLPPVSGGR"
     gene            4471..5385
                     /locus_tag="VIX62_00815"
     CDS             4471..5385
                     /locus_tag="VIX62_00815"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY5858810.1"
                     /translation="MRFQHVLLIVNPVARTVSKPTLAVIEKALSADFRLEVIETKERG
                     HANEIAAQAVHEGVDLVVVFSGDGTINEVVNALAGTETALGVIPGGATNILVRALGLP
                     TNPVDATGVLIGKALDDGAFRLHLGRADGRYFAVNCGAGVDAAAMHRMDTKYPRTKAK
                     FDRVAFRSVAWELMVGYAGRMPDLEVAIDDRPATPSLSVMVGRTDPYTYYKDRGLRMT
                     PQASLDSGLDVLSIRKLARRSLPRIAWQIFGSARHVQGRDIDYVHDASRVVVTSSEEF
                     PVQVDGDSMGGRTRFEIELAREALWVVA"
     gene            complement(5634..6383)
                     /locus_tag="VIX62_00820"
     CDS             complement(5634..6383)
                     /locus_tag="VIX62_00820"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY5858811.1"
                     /translation="MHTLLPDDELVALNLAFEDAHPREILTWALETAAFPRIAIASAF
                     QTEGTITIHMATSIMPDVPILFLETGYQFAETLAFKEQLTDQLGLNVLDLYGEYTVAR
                     QSEELGERLYERDPERCCDLNKVQPMFQALRDLDAWVTAFRRDSSPTRANAPFVEQYE
                     LEPGRWIVKVNPMANWTHDQVWDYLREHDLPHNPLYDLGYTSIGCAPCTRLRFEGEPE
                     RAGRWAGRAKWECGIHEREAAQQRRITLDDA"
     gene            6554..>7167
                     /gene="sat"
                     /locus_tag="VIX62_00825"
     CDS             6554..>7167
                     /gene="sat"
                     /locus_tag="VIX62_00825"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF025664.4"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEY5858812.1"
                     /translation="MTEHDQITPHGGTLVNLLVGTDAAAALSAEAANLPKLVVGEREL
                     SDLEMLAVGALSPLTGFQGEADYRSVLDSMRLANGLPWAIPVVLSVSEDQAHQFGSGG
                     SVALLPAEGADALAVLRVSETFKRDKAKEAVSVYRTDDTEHPGVKAVYEAGDYCVAGT
                     LEVISLPPHDDFQNYRLTPAETRAVFAERGWKTVVGFQTRNPIHR"
BASE COUNT         1122 a         2458 c         2409 g         1178 t
ORIGIN      
        1 atggccagga aggtagtcgg ttagtcgccg gggttgcatc accgctgccg gcccgctctc
       61 cagggcacga ttcaccacga tctcggccac gagggcgccg agcaggtatc cgacgagcat
      121 cgtggcgacc gcccacgccc cctcgtcgaa tgtccgacct gagatcgcgg agtagagcca
      181 cggcgacacg aagccaacga gggcgccgat ccttcgaccg cgcctgctcc agtcgaggta
      241 gcggcggacg tacggcgcct gctccggggt gatctccacc cccgttgcat cggcccacct
      301 ggcgatgtac tcgtcgtcac tctggagtcg tctgctgatc cagaacccga tagccacgac
      361 gaccacgaca acgacgaaga gctcggtcgg gcccatcatc ggcgcctcct cggcgtgcta
      421 gttcatatca acgtatcgat acaatcgcac gattcgatgg cgcggtcaaa tcccggggct
      481 gcggatccct tcgacgacgg gtcacacgtc gtagtacagg ctgaactcgt acgggtgcgg
      541 ccgcagggcc accgcgtcgt gttcctcgat ccgcttgtac tcgatccagg tgtcgatcac
      601 gtcctgggtg aacacgccgc cctcgagcag ccactggtgg tcggcctcca gctcgcccag
      661 cacctcgagc agcgaacctg gcacctgctt cacggccgcg tgctcctcgg gcggcaggtc
      721 gtacaggtcc ttgtcgatcg ggtccggcgg ctcgatcttg ttcttgatgc cgtcgatccc
      781 cgccatgagc atcgcgctga acgccaggta cgggttgcac gacgggtccg gcgtgcggaa
      841 ctccaggcgc ttggcggccg ggctcttcga ccccagcggg atccgaacgc aggcgctgcg
      901 gttccgctgg ctgtacacga gcttgatcgg tgcctcgtag ccgggaacca gccggcggta
      961 ggagttcgtc gtggggttcg tgaacgccag gagcgacggt gcgtgggcga tcagtccgcc
     1021 gatgtaccag cgcgcggcgt cgctgatgcc gccgtagccc acctcgtccc agaacaggtt
     1081 ctcgtcgccg gtccacagcg actggtgggt gtgcatgccc gacccgttgt ccatgaacag
     1141 cggcttgggc atgaaggtca cggtcttgcc ggcctggtac gccgtgttct tgaccacgta
     1201 cttgtacttc atcacgttgt ccgccgtggt caacagcgtg tcgtaacgca tgtcgatctc
     1261 ggcctggccc gcggtgccga cctcgtggtg atgcacctcg atggtgaggc ccaccttctc
     1321 caggttcagc accatgtcgg tgcgcaggtc ctggtacttg tccatcgggg gaagcgcgaa
     1381 gtacccgccc ttgtagcggg gcttgtagcc caggttgccg ccggtttctt ccttgccggt
     1441 ctgccaggcg cccgccaccg agtccacgtg gtagtagccc tcgaactcgt tctggtcgta
     1501 gcggatcgag tcgaagatgt agaactccgc ctcgggtccc cagtacgaac gatcggcgat
     1561 gccggtctgc ttgaggtaca gctcggcctt cttcgcgatg ttgcgggggt cgcgtgaata
     1621 gtcctcgccg gtcactgggt ccttcacgaa gcacgtgatg ttcatggtgg ggtgcttggt
     1681 gaacggatcc atgaacgcgg tgtcgcagtc gggcatcaac agcatgtccg actcctggat
     1741 ctcctggaac ccgcggatcg agctgccgtc gaacggctgg ccctcggtga gcttttcctc
     1801 ggtgagctcg tgcaccggca ccgacaggtg ctggacctga cccggcaggt ccgtgaagcg
     1861 gaagtccacg atctgggctc cctgttcctt cgccatcgcg atgacgtcct tcggtgatgc
     1921 cacgccttcc cccttgcctc gaccgacgtt ccgccaaggc taggagccga acagcggcgg
     1981 cctgttacct cggcgttaac tctgcgttac gagccgtcga tcgctcccat ccgcccgggc
     2041 aggcggggct acggttcggc ccgacggatg ccgatacccg cgggttcacc ttggccgcga
     2101 tgcgagaggg agggcggcat ggacgaacgg ctcgcagccc acgacgatcc ccaagactgg
     2161 gaccttccgg attggttgtt cgacgaccag ggcgagatga tccgcgaggt cgaagacacc
     2221 gaggacgacc gcttcgccga cgcctcctga gcctgcgata ggcgaacggc ccccgagccc
     2281 gagggggtgg gcgaggggcc gttctgggtt gagcttgcca gctcgcgtcg acgcgacggg
     2341 tgcgggccac gaagcgccga cgcgagccgg gtgaacggaa ggctcagggc gcgagaccga
     2401 gggggatgag tcccgagagc gggacgagca tcgcgccttc gtgctctccg ttcatgcccc
     2461 agacatcggc cgcgaagcgc tctacagcgc agagcccgag cgggtcattt cgggggtgac
     2521 gccgacgtgc taggtcgtgg gtcctggacc gttcggtccc ggtgcgccgg cgccgtccgt
     2581 ggccagatgc cgcgtgatgg ctgccgatcg accgtgcctc ggaggccggc gagcatcgac
     2641 ggtccgacct cagtcggggt cggcggctcg gtaccccacg ctccgaacgg tctcgatcag
     2701 gtgctcgtgc tcgggtccga gcttggctcg cagccgccgc acgtgcacgt ctacggtgcg
     2761 cgtgccgccg tagaagtcgt agccccagac ctcccgcaag agcgtcgggc gcgtgaacac
     2821 tcgcccaggg ctcgatgcca ggaaccgcag cagctcgaac tccttgaacg tgaggtccag
     2881 ggagcgtccg ttcgcggtga cgcggtaggt ctcggtgtcg agcgccagcg cgccgagccg
     2941 gatcaccgac ccgtcgcccg cgcccgagcg gcgccgcagc atcgccaggc gaacccgcaa
     3001 ctcgccctcg ggcgcccccg gatacacgaa ctcgtccgcg acctcgtgcc acggatagcg
     3061 ctcgagctgg tcccgctcga cgatcaacat cgccggcacg cggtcgtcgc gcccacgcag
     3121 ttcctgcaac acactccatg cctgcgccgg gttctcgacg gcgtccacga gcaccgcggt
     3181 ggggtcgaac ccgacgacgt ggtcgagcga ggcgagcgac agcggctcga gcttgaggtc
     3241 ggggaggagg tcacccagcg cgggcagcgc cacggtgggg gagcgatccg acagcaacgc
     3301 caggaaggcc acggtgtctc cacgattgcg agtgggaacg gtcgaactcc acgataccgg
     3361 gtccgcatcg gtgccggtag gcttgacctg cgatgaagac ggttcagggc atcacctccc
     3421 atcactggcg agtgcccgcg tatccgtaca cggttaccac ccaggacggc gtcgagatcg
     3481 ccggaacccg cctgggcgaa cccggcgccg acctgccggc cttgatcctg tcccacggca
     3541 tgatcggctg gcaccgcaag cccaagttcg cggtgttcgc cgagaagctc acgccgtggt
     3601 tcgcggtcta tgccatggac atgcgcggac acggcgagag caccggcgtc tgcgacttcg
     3661 gcggcctgga gatctacgac atcgaggccg tgcaccagcg ggcacgcgcc gacggccacg
     3721 agcgcatcat caccgttggt acctccatgg gcgggatcgc cacgatccgg cacggcgcgc
     3781 tgatcggcgg cgtcgacgcc gtggtcggga tctcgtcgtt ggcgtactgg gattggcatg
     3841 acgatgccca tcccaagctc cgtcgcaaca tgcatacgaa gatcggcacg cccgccggcc
     3901 gggccgcgct gcgcgccttc ggtgtccgca tgcccgagca ctgggacgaa cccgaatcgc
     3961 ccgaagaggt gatcggcaag atcgccccca cgccggtgat catcgtccac ggcaccgacg
     4021 acaagctgtt ccctccctcg cacgctcagc ggctgttcga cgccgcagga gaacccaaac
     4081 ggctcttgct cggcgagggc ttcggccatg cggaagacgg actctcgccc gcgttcgcga
     4141 gcaagctcgt cagcgtagtc cacgaatcgg tgggttgggc atggtccggg tgaggttgtt
     4201 cgcggccctc cgcgagcagg ccgacgcctc ccgggtcgaa gcctccggcc gcacggtggg
     4261 cgaggtggtc gaccagctgt ccgagcgcta cggcgagcgg ttcgcctcga tcgccgccgt
     4321 ggggtcctac gtcgtgaacg gcgaacgcgc gagccgggac acggtgatcg ccgagggcga
     4381 cgaggtcgcg ctgctcccgc ccgtgagcgg cggccgctga gcccctaagg gcgcagcgct
     4441 ccccagggtg tctgtgcata ctggcgcgta gtgcggttcc aacatgtcct gctcatcgtc
     4501 aacccggtcg cccggacggt gtccaagcca acgctggccg tgatcgagaa ggcgctctcc
     4561 gccgacttcc gcctcgaggt gatcgagacc aaggaacgcg gccacgccaa cgagatcgcg
     4621 gcgcaggcgg tgcacgaggg cgtggacctc gtcgtggtct tctcgggtga cggcacgatc
     4681 aacgaggtcg tgaacgcgct cgccggcacc gagaccgcgc tcggggtgat ccccggggga
     4741 gccacgaaca tcctggtgcg cgcgctcggc ctgccgacga atccggtcga tgccaccggc
     4801 gtcctgatcg gcaaagcgct cgacgacggc gcgttccgcc tgcatctggg gcgagccgac
     4861 ggccggtact tcgcggtgaa ctgtggcgcg ggcgtcgatg ccgccgccat gcaccgcatg
     4921 gacacgaagt acccgaggac caaggcgaag ttcgaccggg tggcgttccg ctcggtggcg
     4981 tgggagctga tggtgggtta cgcaggccgc atgcccgacc tcgaggtggc gatcgatgac
     5041 cggccggcga ccccatcgtt gtccgtcatg gtgggacgca ccgacccgta cacctactac
     5101 aaggaccgcg ggctccggat gacgccgcag gcttcgctgg acagcgggct cgacgtcctg
     5161 agcatccgca agctcgcgcg gcgaagcctc ccgcgcatcg cgtggcagat cttcgggtcg
     5221 gcgcgccacg tgcagggccg tgacatcgac tacgtgcacg acgcctcacg cgtggtggtc
     5281 acctcctcgg aggagttccc cgtgcaggtc gacggcgact cgatgggcgg gcgcacgcgg
     5341 ttcgagatcg aactcgcgcg cgaggccctc tgggtcgtcg cgtagcccgc gcgccgggag
     5401 gcccccctgc atcccgcaag cggtatcgga tggctcgcac cctgtgcaga tgccgcttcg
     5461 tctcgcgact gacatgcgag atggctggct ggggggacga ggtcccgctg cctatcccac
     5521 ttgcggcatg caatgagggt cgcaaacgcc ttcagccgaa cgcatctcag ttccggtgta
     5581 atagcctgcc gcccgatcta gcgaaatcga ccctcagcac ccgccccgtc gcctcaggcg
     5641 tcgtcgaggg tgatccgacg ttgctgcgcc gcttcgcgct cgtggatccc gcactcccac
     5701 ttcgcacgtc ccgcccaccg gccggcgcgt tcgggctcac cctcgaagcg cagccgggtg
     5761 cacggcgcgc agccgatcga ggtgtacccg aggtcgtaga gcgggttgtg cggaaggtcg
     5821 tgctcacgca ggtagtccca cacctgatcg tgggtccagt tcgccatcgg gttcaccttc
     5881 acgatccagc ggccgggctc gagctcgtac tgctccacga acggcgcgtt cgctcgcgtc
     5941 ggtgacgagt cgcgacggaa cgccgtcacc cacgcgtcca ggtcgcggag cgcctggaac
     6001 atcggctgca ccttgttgag gtcgcagcac cgttcggggt cgcgctcgta gagccgctcg
     6061 cccagttctt cggactggcg cgcgaccgtg tactcgccgt acaggtcgag cacgttcagc
     6121 ccgagctggt cggtgagctg ctccttgaac gcgagcgtct cggcgaactg gtagccggtc
     6181 tccaggaaca ggatgggcac gtccggcatg atcgaggtgg ccatgtggat cgtgatggtg
     6241 ccctcggtct ggaacgccga cgcgatcgcg atccgcggga acgccgccgt ttccagcgcc
     6301 cacgtcagga tctcgcgcgg gtgcgcatct tcgaacgcca ggttgagggc gacgagctcg
     6361 tcgtcgggga gcagggtgtg caaggaggcc tttccacgct cgggtcccgc tcgggtcggg
     6421 cgagcatacc gcagcccttt cgcactcgcc gacagggctt cgtccccgcc gtttccgtac
     6481 actggcgccg cgcggctgct ggagcgcccg cgcgcgaaac cccccggaac cacgtctgaa
     6541 ggagtcgaca cgcatgactg agcacgacca gatcacgccg cacggcggca ccctcgtgaa
     6601 ccttctcgtc ggcaccgacg ctgcagccgc gctgtccgcc gaggccgcca acctgcccaa
     6661 gctcgtcgtg ggcgagcgag agctctccga cctcgagatg ctggccgtcg gcgcactgag
     6721 cccgctcacc gggttccagg gcgaggccga ctaccgctcc gtgctcgact cgatgcggct
     6781 ggcgaacggc ctcccatggg ccatccccgt ggtcctgtcg gtttccgaag atcaagccca
     6841 ccagttcggt tccggtggca gcgtggcgct gctgcccgcc gagggcgccg acgccctggc
     6901 cgtcctccgc gtcagcgaga ccttcaagcg cgacaaggcc aaggaagcgg tctcggtcta
     6961 ccgcacggat gacaccgagc acccgggcgt gaaggcggtc tacgaggccg gcgactactg
     7021 cgtcgccggc accctcgagg tgatctcgct gcccccgcac gacgacttcc agaactaccg
     7081 cctgacgcca gccgagaccc gcgccgtgtt cgccgagcgt ggttggaaga ccgtggtcgg
     7141 gttccagacc cgcaacccca tccaccg
//
LOCUS       DATDBZ010000031         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2875263, whole genome shotgun sequence.
ACCESSION   DATDBZ010000031 DATDBZ010000000
VERSION     DATDBZ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2875263"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VIX62_00830"
     CDS             complement(<1..809)
                     /locus_tag="VIX62_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858813.1"
                     /translation="MPRIADALERESRTVDLEQGDFERLLGRRERKQRNQRIRAGALG
                     VFVALAMGLAFVRSLTSDPVPVDPPVEPTPAPAQPTGAGEVLTRMALPTRKDLVAQDP
                     DSGEVRTIVDARSLPELLPRYRQDITGAAWSSDRRWVAFRRGAGSGAGALWVAYSTQV
                     PAPSWKVAVEVQVWTASADGSTPSLVASQCCVSDGGGPVWSPDGSQIAFETENAGGTA
                     DFRLRYLVVNADGTGEPGEIDGLHVPQLARRLVLLPLLRVKSSLRQHSAMPR"
     gene            complement(802..1326)
                     /locus_tag="VIX62_00835"
     CDS             complement(802..1326)
                     /locus_tag="VIX62_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5858814.1"
                     /translation="MTEPIVVSDAAFDEVEASRTFETFYEAEAQTLFRRLWLVTGNRA
                     EAEEVMQDAFLKVWERWDRVGGMDEPVAYLYRTAMNLFRKRYRRAMLSVRRTVGLAPS
                     VDEFSEADDRQVVRHVLATLPPRQRAALVLTEMLGFMSKEAGDALGVTDATIRSLTRH
                     GRDAFRAVMEVDDA"
     gene            complement(1490..>2012)
                     /locus_tag="VIX62_00840"
     CDS             complement(1490..>2012)
                     /locus_tag="VIX62_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858815.1"
                     /translation="DPETLDIADTYDVPEGGTAVRAGLGGIWITYFAQDRVVRVDPGT
                     GAVVSEIDVGSGPRFLDVGEGGVWVMNQSGGSVSHVDPTSNRVVATIAVDADSIFGGD
                     VMVGAGFVWLRASSELVAQIDPATDRVVARIGRPQASGGVHAGEGQLWIVAHFEKPDG
                     WRAVLYRISLRDG"
BASE COUNT          338 a          697 c          670 g          307 t
ORIGIN      
        1 cggggcatgg ccgagtgctg ccgcagagag gacttcaccc gtagcagcgg cagaagtacc
       61 aaccgccgag ccagctgcgg tacgtggagt ccgtcgatct cccccggttc gcccgttccg
      121 tcggcattga cgaccagata gcgcaaacgg aagtccgcgg tgccccctgc gttttcggtt
      181 tcgaaggcta tctgtgagcc gtcgggcgac cacaccggac ccccaccatc tgacacgcaa
      241 cattgcgacg cgaccagcga tggggtcgag ccgtccgccg aggccgtcca gacctggacc
      301 tccacggcaa ccttccaact cggggcgggc acctgggttg agtaggcgac ccagagggct
      361 ccggcaccgg atcccgcgcc tcgtcggaac gcgacccacc tgcggtcgga tgaccatgcg
      421 gcgccggtga tgtcctggcg gtaccgcggg agcaactcgg ggagcgacct ggcatcgacg
      481 atcgtgcgca cctcaccgct gtcgggatcc tgcgcgacca ggtccttccg ggtcggcagt
      541 gccatcctgg tgaggacctc gccagccccg gtcggctgtg ccggcgccgg cgtgggctcg
      601 acgggcgggt ccacggggac cgggtccgag gtcagcgagc ggacgaaggc gaggcccatc
      661 gcgagcgcga cgaagacacc gagggcgcca gcgcggatcc gctggttgcg ctgtttgcgc
      721 tcgcgtcggc ccagcagtcg ctcgaagtcg ccctgctcga ggtcgacggt gcggctctca
      781 cgttccaggg cgtccgcgat cctaggcatc gtcgacctcc atcaccgcgc ggaacgcgtc
      841 gcgcccgtgg cgcgtcagcg agcggatcgt cgcgtccgtg acaccgagcg cgtcccccgc
      901 ttccttggac atgaacccga gcatctcggt cagcacgagc gccgctcgtt gccgcggtgg
      961 aagcgtggcg aggacgtgcc gaacgacctg gcgatcgtcg gcctcggaga actcgtcgac
     1021 cgaaggcgcg agcccgacgg ttcgccggac cgagagcatg gcccgccggt agcgcttgcg
     1081 gaacaggttc atcgcggtcc ggtagaggta ggcgacgggc tcgtccatcc caccgacgcg
     1141 gtcccatcgc tcccacacct tgaggaaggc gtcctgcatc acctcttcgg cctcggcgcg
     1201 gttcccggtc acgagccaca gccgtcggaa cagcgtctga gcctcggcct cgtagaaggt
     1261 ctcgaaggtg cgggaagctt cgacctcgtc gaaggcggcg tccgaaacga cgatgggttc
     1321 ggtcatgtcg gtgtatgaac cctcgaaacc ccccgagcgt gcaagtggac atgcatgcgg
     1381 gacgaaacct gtaccaggat gacggggtga agcggcgggt cgggaccgaa tcaccgttgc
     1441 catcggttaa tcggggcggc gattcggtgg agtgcaggtc gtatcgtggc tacccatccc
     1501 tgagggagat ccggtagagc accgcccgcc acccatcggg cttctcgaaa tgggccacga
     1561 tccagagctg cccctcgcct gcgtgcacgc ctccgctggc ctgcggccgc ccgatccgcg
     1621 caacgacgcg atcggtggcg gggtcgatct gcgccaccaa ctcggagctg gcacggagcc
     1681 agacgaaccc cgctccgacc atgacatcac cgccgaagat gctgtccgcg tccaccgcga
     1741 tcgtggcgac gaccctgttg gacgttgggt caacgtggga caccgatcct ccgctttggt
     1801 tcatgaccca cacaccacct tcaccaacgt ccaagaaccg tgggccggaa ccaacgtcga
     1861 tctccgacac gaccgcgccc gtcccagggt caacgcgcac gacgcgatcc tgggcaaagt
     1921 aggtgatcca gatcccgccg agcccggcac gcacggccgt gccgccttcc ggaacgtcgt
     1981 acgtgtcggc gatgtcgagg gtttccggat cg
//
LOCUS       DATDBZ010000032         2610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1442060, whole genome shotgun sequence.
ACCESSION   DATDBZ010000032 DATDBZ010000000
VERSION     DATDBZ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442060"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VIX62_00845"
     CDS             <1..561
                     /locus_tag="VIX62_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858816.1"
                     /translation="GQPDMLEAFASTATAAGVRAVPVEGRWPDVAPEVPSADVVMSGH
                     TIYNAPDLVPFVRALHDHARQRVVLEATDRHPLGWMADLWRTFHGLDIPDEPRVELAI
                     EVMNSVGIEPLRQDRPGGDDDPAGGGFTTRDAAVALVRTRLCLRPGDDAAIADALGSR
                     LRLRNGRWSAGPHERTVVTLWWDRAA"
     gene            complement(578..1174)
                     /locus_tag="VIX62_00850"
     CDS             complement(578..1174)
                     /locus_tag="VIX62_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858817.1"
                     /translation="MRRALRTVLWIAVFGACFGVGAYVAAHTDPFPPGVDDPGARPDP
                     DGSPDDPAGGDGWVVQIEMRTYHDLYVGGRCAANWRVEVTLDEVADPALSGTGAAILK
                     GELRCDESTAQIQADRIELEAVGTVRGDEVRFRLDDTARSPVGAQELSGLVKTLPTLR
                     FRMPQRDGATATFDITVPDGDRGTYGAIGDVLLLAPAA"
     gene            complement(1183..>2610)
                     /locus_tag="VIX62_00855"
     CDS             complement(1183..>2610)
                     /locus_tag="VIX62_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858818.1"
                     /translation="PEDIARFKDMLAELNGLIEMRERGEDTQPTFEDFMSRYGDLFPD
                     RPRTLDELLENMAKRMAAMSRLMASLSPEQREELAALSQQVMQDMDLAFEVDRLSANL
                     SDAFPDMGWGEPAMGDGDDAMPMSAAVDAMERLHDYEDLERAMEGDYAGAALDDVDED
                     ALRRTLGDTAAQDLRRLKEIERALEKAGLLQRRKGRLEVTPRGARRLGERALTTIFEE
                     LKRDREGTHEARDPGGMAEPTGATRPWVFGDHGQLAVQKTVFNAVVRSEPGEPVRLRP
                     DDFEMIEAEQRTEVATALLLDLSFSMPLRGHFIHAKKMALALHALIEGRYPNDTLYLI
                     GFSDYARQLQPEDLTAPGFERVYGTNMEHAFLLANRLLSQHPRATRQVIMVTDGEPTA
                     HLEGDQAFFSWPPVRETIERTLTEAMRLSKSGVRLNIFMLEESEGLAAFMEQLARLTH
                     GRVFLMDDHRVGDFVVRDYVTRRSR"
BASE COUNT          415 a          910 c          850 g          435 t
ORIGIN      
        1 ggacagccgg acatgctcga ggcgttcgcg tcgacagcga ccgcggcggg tgtgcgagcg
       61 gtgcccgtcg aaggtcgctg gcccgacgtc gctcccgagg tgccgtcggc cgacgtcgtg
      121 atgtccggtc acacgatcta caacgcgccc gacctggtgc ccttcgtccg cgccctgcac
      181 gaccacgccc ggcagcgggt ggtgttggaa gccaccgatc gccatccgct cgggtggatg
      241 gccgacctgt ggcgaacctt ccatggcctg gacatccccg atgaacctcg cgtggaactc
      301 gcgatcgaag tcatgaactc ggtcgggatc gagccgctgc ggcaggatcg acccggtgga
      361 gacgacgacc cagcgggcgg tggtttcacc acccgggacg ccgctgtggc gttggtgcgg
      421 acccgcctct gtctgcggcc cggggatgac gcagcgatcg ccgatgcgct cggcagccgg
      481 cttcgcctgc ggaacggacg ttggtcggcc ggaccacatg agcgaacggt cgtcaccctg
      541 tggtgggatc gagccgcctg aacgacaccg gccctgccta ggcagcgggt gcgagcaaga
      601 gcacatcccc gatggcaccg tacgtccccc ggtcaccgtc gggcacggtg atgtcgaacg
      661 ttgccgttgc cccgtcgcgc tgcggcatcc ggaaacgcaa ggtcggcagg gtcttgacga
      721 gtccggagag ctcctgtgcc cccaccggcg accgcgcggt gtcatccagc ctgaatcgga
      781 cctcgtcgcc tctcaccgtc cctaccgcct ccagttcgat gcgatcggct tggatctgtg
      841 cggtggattc atcacaccgg agctcacctt tcaggatcgc agcgccggtg ccgctcagcg
      901 ccgggtcggc cacctcatcg agcgtgacct cgacgcgcca gttggccgcg cagcgaccgc
      961 ccacgtagag gtcgtgatag gtgcgcatct cgatctgcac cacccatcca tcgcctccag
     1021 cagggtcgtc cggtgagccg tcaggatcgg gacgagcgcc gggatcgtcg acccccggtg
     1081 ggaacgggtc ggtgtgggcg gccacgtacg ccccaacgcc gaagcacgcc ccgaagacgg
     1141 cgatccacaa cacggtcctc agggcgcggc gcatgcccga ccctaccgcg agcgtcgcgt
     1201 cacgtagtcg cgcaccacga agtcccccac ccggtgatcg tccatcagga acacccgtcc
     1261 gtgggtgaga cgcgccagtt gttccatgaa cgccgcgagg ccctcggact cctcgagcat
     1321 gaagatgttg agacgcaccc cggacttgga aagccgcatc gcctcggtga gggtgcgctc
     1381 gatcgtctca cgcaccggcg gccacgagaa gaacgcctgg tcaccttcca ggtgcgccgt
     1441 gggctctccg tcggtcacca tgatcacctg gcgggtcgct cgaggatgtt gggacagcag
     1501 acggttcgcc agcaggaacg cgtgctccat gttcgtgccg tacacgcgct cgaagccggg
     1561 cgcggtgagg tcttcgggct gcagttgccg cgcgtagtcg ctgaacccga tcaggtacag
     1621 ggtgtcgttc gggtaacgac cttcgatcag ggcatgcagc gccagcgcca tcttcttcgc
     1681 gtggatgaag tgaccgcgaa gcggcatcga gaacgacagg tccagcagca acgccgtggc
     1741 gacctccgtg cgctgttcgg cttcgatcat ctcgaagtcg tcgggtcgca ggcgaacggg
     1801 ttcaccgggc tcagaccgga ccacggcgtt gaagacggtc ttctgcaccg caagctgtcc
     1861 gtgatcgccg aacacccaag ggcgagtggc gccggtgggc tcggccatgc caccaggatc
     1921 tcgagcctca tgcgtcccct cgcggtcgcg tttgagttcc tcgaagatcg tcgtcagggc
     1981 acgttcgccc aggcggcgtg ccccacgcgg cgtcacctcc aatcgcccct tgcgccgctg
     2041 cagcaggccc gccttctcca gtgcgcgctc gatctccttg aggcgtcgta gatcctgggc
     2101 agccgtgtcg cccagcgtcc ggcggagggc atcctcgtcg acgtcatcca gggcggcacc
     2161 cgcgtagtcg ccttccatcg cgcgctcgag gtcctcgtag tcgtgcaggc gttccatcgc
     2221 atcgacggcg gccgacatcg gcatcgcgtc gtccccatcg cccatcgccg gctcacccca
     2281 ccccatgtcg gggaacgcgt cggacaggtt cgcgctgagc cggtcgacct cgaacgcaag
     2341 gtccatgtcc tgcatcacct gctgggagag cgcggccaac tcctcacgtt gctcgggcga
     2401 caaggaggcc atgagccgac tcatcgccgc catacgcttc gccatgttct cgagcaactc
     2461 gtccagggtc cgcgggcgat cggggaacag atccccgtag cgcgacatga agtcctcgaa
     2521 ggtcggctga gtgtcctcgc ctcgctcgcg catctcgatc aagccgttga gctcagcgag
     2581 catgtccttg aacctggcga tgtcctcggg
//
LOCUS       DATDBZ010000033        11208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1442296, whole genome shotgun sequence.
ACCESSION   DATDBZ010000033 DATDBZ010000000
VERSION     DATDBZ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442296"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1078)
                     /locus_tag="VIX62_00860"
     CDS             complement(149..1078)
                     /locus_tag="VIX62_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5858819.1"
                     /translation="MRVALFGCGWVQEFHARGALACGAELVAVANHREATAASFAERH
                     GIPRVTTDWEALARDPDIDAAIVATPNALHAPQSIALLEAGKHVMVEKPMAVSVAECD
                     AMIEASRRSDTSLMVAHCWRFREEVIAVRDRIAAGELGDVVKTRGYGVHANWGPSGWF
                     TDRSLAGGGSLVDMGVHAIDTARFLLGDPEPTQVFATIGTRYGAYDVDDDTILLISWS
                     QGTNSIVESGWWHRHAEGMEADTEVYGTRGYGRIFPREAPSEDYEHCTQPMYSAQMAE
                     FLGAVAEGRQPRPTGDDGRVVMRVVEAAVAAAR"
     gene            complement(1100..2011)
                     /locus_tag="VIX62_00865"
     CDS             complement(1100..2011)
                     /locus_tag="VIX62_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY5858820.1"
                     /translation="MTTQQATAPQDAVVDSVPALIPRPVPPDVRRRRFLMAVANHTIL
                     IAVAAMFTLPFLFVLLTSVMTTDQALTTELWPRPFVWSNYAEVFERLPVFRYAFNTMT
                     IAVLSTVGVLVSSIPVAYALARIRWRGRQAAFVVILATIMLPYQVTIVPLYVVFARYG
                     WIPSFKPLIVPLFFGDAFSIFLLRQFFMTIPEELSDAARVDGGSEWQIMTRVIVPLAK
                     PAIAAVALFQFLYSWNDFFAPLLFLSDDSSLWTLAIGLNEFRGLHQVEYNLMLAASVL
                     FMLPVIVLFFLAQRVFIEGVTLTGVKG"
     gene            complement(2008..2895)
                     /locus_tag="VIX62_00870"
     CDS             complement(2008..2895)
                     /locus_tag="VIX62_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY5858821.1"
                     /translation="MLLFLSPWIVGFLAFTLYPMVSSLYFSFTSYDLLSTPRWIGLEN
                     YRFMFTKDPVFWTAIKNTAWIIIVGVPLRILAAIVTASLLVRPRKGVKGYRTMFFVPT
                     LAPAVAAALAFLYLFNPATGPVNQVLSGIGIKDPPLWLYDAQWSKPTLVLLILWGVGD
                     AMIIFLAGMLDVPRQLYEAAEIEGASGWQQFRSVTLPLISPVIFFSLVIGVIYGFQYF
                     TQPYVISFWLTGDAAQIGHPQNSLLFFTSRLYQMGFQNFKMGYASALAWVLLVVTMIC
                     TLVILRNSKRWVHYGGGLR"
     gene            complement(2997..4358)
                     /locus_tag="VIX62_00875"
     CDS             complement(2997..4358)
                     /locus_tag="VIX62_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5858822.1"
                     /translation="MRRLVVATAVLTFVAAACTAGGGDEPAPTVDTAASNEPVTVTLT
                     GEWTSKRECDEWQNSFKENFEAEYDWITVDAKCGVTEEKQIAAINAGNPPDAFLSFGV
                     DNVGRFCDSGAWVDLNPYIDGPDGVDRSVFVDAALTYTSFEGTQCSLPFLTDTYGLYY
                     NLDLFKAAGLTDPPKTTDELTEDAKKLTEFNPDGSIKVAGFVPVTDYYCCSSNLVNMS
                     HMFGASYLDEGGSPAFASDPAWAEMLQWQHDFIAEVYGEGDFQTGAEKLRKFVAGAGN
                     EWGNPQDFQQGRVAMMIDGEWRTAFMTDFAPEVNYATAPLPTSPSRTDLYGSATAGGT
                     IVGIPKGAEHPAEAWLLVKYLATNTDTLVYMANWVGNVPTTHEAIDSPDLDLPEQFGV
                     FMEAFAHPDSAWRPTTVLGEELEQYLGNFTSDWQTGDATDLEKGLQDATEQTQDALDQ
                     AQL"
     gene            complement(4445..5572)
                     /locus_tag="VIX62_00880"
     CDS             complement(4445..5572)
                     /locus_tag="VIX62_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY5858823.1"
                     /translation="MLDCVLRMGPISRAQIARETALSKPTVSQALAGLELAGLVHEAG
                     RSKGGKGPTAILYELNHRAGWVVGIDVGRDWVRAAIADVTGEFVARRDERARVKSAPT
                     LITQIGAIAHGLAADAGIRWRQVTFATVGSPGVFEPERGQVALAYSLPGWGREGLVEM
                     VQAELGTKTAFENDVNLAALGEQWHGLGKGVGNFVYLHLGTGVGMGLVLDGELFRGST
                     GAAGEVGYMPLAGTDLKDPTSRRRGPLDAAASAAGVVATARKLGMLPPLTAKRVFNAA
                     RAGDAKARRVVRMEAQRIALAVAAVASVVDPELVILGGGIGGNGDLLLEPVERELRSI
                     SPFRPRVEASVLREEATLYGSVFMALQAAQDQLFARGKVPA"
     gene            5769..6383
                     /gene="eda"
                     /locus_tag="VIX62_00885"
     CDS             5769..6383
                     /gene="eda"
                     /locus_tag="VIX62_00885"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY5858824.1"
                     /translation="MTIIESGPLVGIVRYRATADLIPVLEALADGGIPLLEVTLDTPG
                     ALDAIASIAGTGTTIGAGTVLDGDQVRASADAGAGFVVSPGLVDAVVEQALALGIEPI
                     PGVLTPTELLRARRLGASAVKIFPAGPCGGPELIRALRSPFPEVGLLATGGIAVDDVG
                     AYLAAGSTAVGLGSALTGHRPPETPAELDALRARAAAALEAARR"
     gene            6380..7279
                     /locus_tag="VIX62_00890"
     CDS             6380..7279
                     /locus_tag="VIX62_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY5858825.1"
                     /translation="MSGTTYDVIGLGETMLSLVARDGPLESASAFAATHGGAESNALV
                     ALSRLGFRTAWISRLGDDPAGTRVREALEVEGVDLRWVRTDPARFTGLMLRDTRGGVR
                     YWRTGSAASALDPAALDGVPIADARAVLVTGITAMLGAGPQAAAIALLDRAGGLRAVD
                     PNLRPGLWGSDRAHELVMPLLERCDVVLGGERELASLVGGSGAGSARRCAELGPNEVV
                     VKRGADGAAVLEPDGAWRELGPSASDDVDPVGAGDAFNAGYLAARLDGASCLESLRAG
                     AAAGAAAAGVLGDVGARTQDVDV"
     gene            complement(7320..8609)
                     /locus_tag="VIX62_00895"
     CDS             complement(7320..8609)
                     /locus_tag="VIX62_00895"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF025397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY5858826.1"
                     /translation="MTGGRRLDLRAVLAVRVEETTTGFPAGADLAVADVGSRGWNLFE
                     DLVPPVVVLRERALEHNLELMSAYCRANGVELAPHGKTSMAPQLWARQLDAGAWGVTA
                     ATPVQARAMRSAGVPRILLADELVDAGSIGWVARELADEAFGFACWVDSTRGVELLRE
                     GLVAGGAPRPLSVLVELGHARGRSGCRSVAEALDLAALVHEAPDLALVGVAGYEGTVC
                     GDRGPVCVDAVDAFLHDLRELTSELLARGAFDTAGEAIVSAGGSAFFDRVVAVLGKSS
                     AAGAAVRLVLRSGCYLTHDHGVYERNSPLSAATDPGGRFLPAFEAWGAVLSRPETRVV
                     VVGLGKRDVPSDIDLPVPLRIRRSTGSFTDAAGIVVRRLMDQHAICDVGGSGAPPDVG
                     DLVAFGVSHPCTAFDRRRVIPVVDDDDRVVDAVATLF"
     gene            complement(8606..9907)
                     /locus_tag="VIX62_00900"
     CDS             complement(8606..9907)
                     /locus_tag="VIX62_00900"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 4"
                     /protein_id="HEY5858827.1"
                     /translation="MGARVVVIGGGSAYMPGIAFAFANHAEAFGDATLVLQDIDAGVL
                     DLQRRLTGSILRSRGAGGVRVEAHADRSPALEGADVVLAAFRPGGLEARHLDERIAID
                     HGVIGQETAGPGGFAMALRSVPIVLEIAEEMRRLAASEAVLLDYTNPVQVVSEAMRRF
                     GPALPFLGLCDQTAGEARFLARLLGVDADVIALDTCGTNHMTFTRGVRVASEDVTREL
                     WTLLDSLPLERLTSDAERRIVQLFRTLHHVPSEYMQYFFFHDEVLAEQRARGRTRAQE
                     VMAILPSVLESYRREADAEDPRPSMARASEEHGDFAVSVMAAMLTDTHRRAILNLPNA
                     GQVDDLPDATIVETPATIDGRTVSPVPQGALPVEVSGLVRQVAVHAAMTAEAAVTGDR
                     ELAVTALAVHPLVRSVNLADALVGAYLDAHAAHLPAGWGRR"
     gene            10029..>11208
                     /locus_tag="VIX62_00905"
     CDS             10029..>11208
                     /locus_tag="VIX62_00905"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5858828.1"
                     /translation="MAELAVNGGTPVRSTPYPAWPAPDDEYVAAVSEVVRGGEWGGFP
                     EPGVNAAAFEEAFAAYQGARHGVLMMNGTVTMEVACKALDVGWGDEVILPALTFAATA
                     YAPMAAGALPVIVDVTPETWCIDPDRVEAAITPRTRAIIPVHLGHQMADMDRLTEIAD
                     RHGLAVIEDCAHAHGQEWRGRGAGCIGDFGSFSHQSSKILTSGEGGTLLTNDDTLARR
                     AHSIIDCGRAKDAEEKEFTFGANYRLSELNAALLVVAMRRFPGQQEERATNGLRFERL
                     AADIPGVRVMPRDGRITRWSFYNYILAIDPGAFGGATNEIVCAALEAEGIPAEVQYPP
                     MSRYELFQPSLSRLPVAVEHADRLDPRGMSFPVAEAAGLRESVYLMEAVFRDGERGVQ
                     D"
BASE COUNT         1813 a         3975 c         3767 g         1653 t
ORIGIN      
        1 tcgcacctgc ccgttcacgg tcacggacac gttcgtcacg gacggatccc ccttcccgcg
       61 gcggtgcctg ccagcctcag ccggcgtggt cggaacctgg ctagggcacc ggagtgtagg
      121 gcaggtgtgg aaaacgcgcg agccgacctc agcgggcggc cgcgaccgcc gcctcgacca
      181 cccgcatcac gacccgtccg tcgtcgccgg tcggccgagg ctgcctgcct tcggcgaccg
      241 ccccgaggaa ctcggccatc tgcgcgctgt acatcggctg cgtgcagtgc tcgtagtcct
      301 cgctcggcgc ctcgcgaggg aagatgcgac cgtacccacg ggttccgtac acctcagtgt
      361 cggcctccat gccttcagcg tgccggtgcc accacccgct ctcgacgatc gagttcgtcc
      421 cctgggacca ggagatcaac aggatcgtgt cgtcgtcgac gtcgtacgcg ccgtaccggg
      481 tgccgatcgt ggcgaacacc tgcgtcggct cggggtcccc caggaggaac cgcgccgtat
      541 cgatcgcgtg cacgcccatg tcgaccagcg acccgccgcc ggcgagggag cggtcggtga
      601 accatccgga cggcccccag ttcgcgtgca cgccgtagcc tcgcgtcttc acgacgtcgc
      661 cgagctcgcc agccgcgatc cggtctcgca ccgcgatcac ctcctcacgg aagcgccagc
      721 aatgcgcgac catcagggac gtatccgaac ggcgcgacgc ctcgatcatc gcgtcgcact
      781 cggcaaccga caccgccatc ggcttctcca ccatcacgtg cttcccggcc tcgagcagag
      841 cgatcgattg cggggcatgc aacgcgttcg gggtcgcgac gatcgcggca tcgatgtcgg
      901 gatcgcgagc caaggcctcc cagtcggtgg tcacgcgcgg gatgccatgg cgctcggcga
      961 acgacgcggc cgtcgcctcc cgatggttgg cgaccgccac cagctcggcg ccgcacgcga
     1021 gtgcgccacg ggcatggaac tcctgcaccc acccgcaccc gaacagcgcg accctcatcg
     1081 gcgtcccacg ccgggcaacc taccccttga cgcctgtcaa cgtgacccct tcgatgaaga
     1141 cacgttgcgc gaggaagaac agcacgatca ccggcagcat gaagagcacc gacgcggcca
     1201 gcatcaggtt gtattcgacc tggtggagtc ctcggaactc gttgaggccg atcgcgaggg
     1261 tccacaggct cgagtcgtcg ctcaggaaca gcaagggcgc gaagaagtcg ttccacgagt
     1321 acaggaattg gaacagcgcg accgccgcga tcgccggctt cgcgagtggc acgatgacac
     1381 gcgtcatgat ctgccactcg ctgcccccgt cgacccgggc cgcatcggag agctcctcgg
     1441 ggatcgtcat gaagaactgt cgcaacaaga agatcgagaa cgcgtcaccg aagaacagcg
     1501 gcacgatcag tggcttgaat gacgggatcc acccatagcg cgcgaacacc acgtagagcg
     1561 gcacgatcgt cacctggtag ggcaacatga tcgtcgcgag gatcacaacg aacgcggcct
     1621 gccggccgcg ccaccgaatg cgggcgagcg cataggccac cgggatgctc gagaccagga
     1681 cgcccaccgt cgagagaacg gcgatcgtca tcgtgttgaa cgcgtagcgg aagaccggga
     1741 gccgctcgaa cacctcggcg tagttcgacc agacgaacgg cctgggccag agctccgtcg
     1801 tcagtgcctg atcggtggtc atgaccgacg tcagcagcac gaagaggaac ggaagcgtga
     1861 acatcgccgc cacggcgatc aggatcgtgt ggttcgcaac ggccatgagg aagcggcggc
     1921 ggcgcacgtc tggcggcacc ggacgaggga tcagtgccgg gaccgagtcg acgacggcgt
     1981 cctgcggagc cgtggcttgc tgcgtggtca tcggagacca cctccgtagt gcacccagcg
     2041 cttcgagttc cgcaggatga ccagagtgca aatcatcgtg accaccagca gcacccatgc
     2101 cagggcggaa gcgtagccca tcttgaagtt ctggaacccc atctggtaca gccgcgaggt
     2161 gaagaacagc agggagttct gcgggtgccc gatctgggcg gcgtcacccg tgagccagaa
     2221 gctgatcacg tacggttggg tgaagtactg gaacccgtag atcacgccga tcacgaggga
     2281 gaagaagatg accggcgaga tcaacggcag cgtcaccgac cggaactgct gccacccgga
     2341 cgccccctcg atctcggcgg cttcgtagag ctggcgcggc acgtcgagca tgcccgcgag
     2401 gaagatgatc atcgcgtcgc cgacccccca gaggatgagc aacacgagcg tcggcttcga
     2461 ccattgcgcg tcgtacagcc acagcggcgg atccttgatg ccgatcccgc tgagcacctg
     2521 gttcaccgga ccggtcgcgg ggttgaagag gtagaggaac gccagcgccg ccgcgaccgc
     2581 cggggccagc gtcggcacga agaacatcgt ccgatagccc ttgacgccct tcctcggacg
     2641 cacgagcaac gacgcagtga cgatggccgc caggatccgc agcggcacac cgacgatgat
     2701 gatccacgcc gtgttcttga tcgcggtcca gaagaccggg tccttcgtga acatgaaccg
     2761 gtagttctcg agcccgatcc acctgggcgt ggagagcagg tcgtagctcg tgaacgagaa
     2821 gtacaggctc gagaccatcg ggtacagggt gaaggccagg aacccgacga tccacggcga
     2881 caggaagagg agcaccgtga ggtagcgcct cctcgtctgg cgccgccgag cgggagacgc
     2941 tcgtcgcgtc ccccgctcga tcggagccgt cgctgcctca gtcgtcgccg ctccccttac
     3001 agctgcgcct gatcgagcgc gtcttgggtc tgctcggtcg cgtcctggag tcccttttcc
     3061 aggtcggtcg cgtcaccggt ctgccagtcc gacgtgaagt tgcccaggta ctgctccaac
     3121 tcctcgccga gcaccgtcgt cggcctccag gccgagtccg ggtgcgcgaa cgcttccatg
     3181 aagacaccga actgctcggg aagatcgagg tccggcgaat cgatcgcttc gtgcgtggtg
     3241 ggcacgttgc cgacccagtt cgccatgtag acgagcgtgt cggtgttcgt ggcgaggtac
     3301 ttcacgagca accaggcttc cgccgggtgc tccgcaccct tcgggatccc gacgatcgtg
     3361 ccaccggccg tcgcggaccc gtagaggtcc gtgcggctgg gtgacgtcgg gagaggagcg
     3421 gtcgcgtagt tgacctcagg tgcgaagtcc gtcatgaagg ccgttcgcca ttcgccgtcg
     3481 atcatcatgg ctaccctgcc ttgctggaag tcctgggggt tcccccactc gttcccggcg
     3541 ccggccacga acttccggag cttctcggcc ccggtctgga aatcgccttc accgtagacc
     3601 tcggcgatga aatcgtgttg ccactgcaac atctccgccc aggcgggatc cgacgcgaag
     3661 gccgggctgc ctccctcatc caggtaggac gccccgaaca tgtggctcat gttcaccagg
     3721 ttgctgctgc agcagtagta gtcggtcacc gggacgaacc cggccacctt gattgaaccg
     3781 tcggggttga actccgtgag cttcttcgcg tcctccgtca gctcgtccgt agtcttcggc
     3841 gggtcggtga ggcctgccgc cttgaagaga tcaaggttgt agtagaggcc ataggtgtcg
     3901 gtcaggaacg ggagcgagca ctgcgtgccc tcgaagctcg tgtacgtcag ggcggcgtct
     3961 acgaacaccg aacgatcgac cccgtccggt ccgtcgatgt acgggttcag atcgacccat
     4021 gccccggagt cgcagaacct gcctacgttg tcgacgccga acgacaggaa cgcgtcgggt
     4081 ggattccctg cgttgatcgc cgcgatctgc ttctcctcgg tcacaccgca cttcgcgtcg
     4141 accgtgatcc agtcatactc ggcctcgaag ttctccttga acgagttctg ccactcgtcg
     4201 cactcgcgct tcgaggtcca ttcccccgtg agcgtcacgg tcaccggctc gttcgacgcg
     4261 gcggtgtcca cggtgggagc gggctcgtcc ccgccaccag ccgtacacgc cgcggccacg
     4321 aacgtgagca cggccgtcgc caccaccaaa cgcctcatcg tgcgcctccc tcccccggtc
     4381 gttcccggga tctcctccga gctgctgatg atcggttgta cgttcgtgag cgatcttccg
     4441 tgcatcaggc cggcaccttc cctctggcga acagctggtc ctgcgccgcc tgcagcgcca
     4501 tgaacaccga gccgtacagc gtggcctcct cgcggagcac cgatgcttcg acccggggtc
     4561 ggaacggcga gatcgaacgc agctcccgtt ccaccggttc gagcaggagg tctccgttgc
     4621 cgccgatgcc gccgccgagg atcacgagct ccggatcgac gaccgaggcg accgccgcga
     4681 cagcgagcgc gatgcgctgg gcctccatgc gcacgacccg cctggctttc gcgtcacccg
     4741 cgcgagccgc gttgaacacg cgcttggcgg tcagcggcgg caacatgccg agcttccgcg
     4801 cggtcgcgac gacccctgcg gcgctcgcgg cagcgtcgag cggaccccgg cggcggctcg
     4861 tcgggtcctt cagatcggtg cccgccagcg gcatgtaccc cacctcgccg gcagccccgg
     4921 tgctgccgcg gaacagctcg ccgtcgagca ccagccccat gcccacgccc gtgcccaggt
     4981 gcaggtagac gaagttgccc acgcccttgc cgagaccgtg ccactgctcg ccgagcgccg
     5041 ccaggttcac gtcgttctcg aaggcggtct tggtgccgag ctcggcctgc accatctcga
     5101 ccaggccctc tcgcccccac cccggcaggc tgtaggcgag cgccacctga ccacgctctg
     5161 gctcgaacac gccgggactc ccgaccgtgg cgaacgtcac ctgtcgccac ctgatgccgg
     5221 cgtcggccgc gagcccgtgg gcgatcgcgc cgatctgcgt gatcaacgtc ggcgcgctct
     5281 tcactcgcgc ccgctcgtcc cggcgtgcga cgaactcccc ggtgacgtcg gcgatcgcgg
     5341 cccggaccca gtcccgtccg acgtcgatac cgacgaccca gccggcccgg tggttcagct
     5401 cgtacaagat ggccgtgggt cccttcccgc ccttcgaccg gcctgcctcg tggacgaggc
     5461 ctgcaagctc gagcccggcg agtgcctgcg aaaccgtcgg cttggacaag gcggtctcac
     5521 gtgcgatctg ggctcgagag atcggaccca tgcgcagcac gcaatccagc acggtgcgtt
     5581 cgttgatcgc gcgaagcagg cttggcgtgc ccgcggccgg agccttccgg tcgaggttcg
     5641 ttgcgcctcc cctcagtgca aaggaaactt tcctaactat tcgggcggat gctacgctcg
     5701 ctctcggacc cgagtcaaga caatcggcgg aaccgccccg accggcgcac ggagggccgc
     5761 gggcaacgat gacgatcatc gagtcaggac cgttggtcgg gatcgtccgc tatcgggcga
     5821 cggctgacct catcccggtc ctggaggcac tggccgacgg cggaatcccg ctcctggagg
     5881 tcacgctcga cacgcccggg gccttggacg cgatcgcatc gatcgcgggg accggcacca
     5941 caatcggcgc gggcaccgtg ctcgacggcg atcaggtgcg ggcgagcgcc gacgccggag
     6001 ccggcttcgt cgtgtcgccc ggactcgtcg acgcggtggt cgagcaggcg ctcgccctcg
     6061 gcatcgagcc gatccccggt gtgctcacgc cgaccgagct gctgcgagct cgtcgcctgg
     6121 gagcctcggc cgtgaagatc ttccccgccg gtccgtgtgg cgggccggag ctgatccgtg
     6181 ccctgcgctc gcctttcccc gaggtcgggc tgctggccac gggcggcatc gccgtcgacg
     6241 atgtcggcgc ctatctcgcc gccgggtcga cggcggtcgg gctcgggtcg gcactcaccg
     6301 gacaccgtcc gcccgagaca cccgccgagc tcgacgcgct gcgagcccgc gccgctgccg
     6361 cgctcgaggc ggcgaggaga tgagcggcac gacctacgac gtgatcgggc tcggcgagac
     6421 gatgctctcc ctcgtcgctc gcgacgggcc gctcgagagc gcgtcggcgt tcgccgcgac
     6481 ccacggcggc gccgagagca atgcactcgt cgccctctcc cgtctggggt ttcgtacggc
     6541 gtggatcagc cggctcgggg acgaccctgc cggaacgcgc gtgcgcgagg ccctcgaggt
     6601 ggaaggggtc gatctccgat gggtccgaac cgacccggcg cgattcaccg gcctgatgct
     6661 ccgcgatacc cggggagggg tgcgctactg gcggaccgga tcggccgcga gcgcgctcga
     6721 cccggccgcg ctggacgggg tgccgatcgc cgacgcccgc gccgtgctcg tgacggggat
     6781 cacggcgatg ctcggggcgg gaccgcaggc cgcggcgatc gcgctcctcg accgtgccgg
     6841 cgggctccgc gccgtggacc cgaaccttcg tccgggcctc tggggatccg atcgcgcaca
     6901 cgagctcgtg atgccgctgc tggaacgctg cgacgtggta ctcggcggcg agcgagagct
     6961 cgcgtcgctc gtgggcggct cgggcgcggg gtcggccagg cgctgtgcgg agctggggcc
     7021 caacgaggtc gtcgtgaagc gcggcgccga cggcgccgcg gttttggagc ccgacggcgc
     7081 ctggcgcgag ctcggtccgt cggcctccga cgacgtcgac cccgtcggcg ccggcgacgc
     7141 gttcaacgct ggctatctgg ctgcccggct cgacggggca tcatgcctcg agtcgctgcg
     7201 agcgggagcg gcggcaggag cggcggccgc gggcgtcctc ggcgatgtcg gtgcgcgcac
     7261 gcaggacgtc gatgtgtgac ccagccctcg tcgcacgaga tcgggcgtcg acgccgcgat
     7321 cagaacagcg tggccacggc atccacgacg cgatcgtcat catcgacgac ggggatcacc
     7381 cggcgacgat cgaaggccgt gcagggatgg gagacaccga acgccacgag gtcgcccacg
     7441 tccggtggcg caccgctgcc gcccacgtcg cagatcgcat gctggtccat cagccgccgc
     7501 accacgatcc cggcggcgtc ggtgaacgat ccggtcgacc tccggatgcg gagcggcacg
     7561 ggcagatcga tgtccgacgg cacatcgcgc ttgcccagac cgacgacgac gacacgcgtc
     7621 tcgggacgcg acaacacggc gccccacgcc tcgaacgccg gcaggaagcg gccgccggga
     7681 tcggtcgccg cggacaacgg agagttgcgc tcgtacacgc cgtgatcgtg ggtgaggtag
     7741 cacccagacc gcagcacgag ccgtaccgcg gcccccgcag ccgagctctt cccgagcacc
     7801 gccaccacac ggtcgaagaa cgcgctccct ccggcgctga caatcgcctc accggcggta
     7861 tcgaaggcgc cgcgagccag cagttcgctc gtgagctcac ggaggtcgtg gaggaacgca
     7921 tcgaccgcgt cgacacacac cggtccacgg tcgccgcaga ccgtgccctc gtagcccgcg
     7981 accccgacga gcgccaggtc cggcgcctcg tggaccaggg cggcgaggtc gagcgcctcc
     8041 gcgacgctcc ggcatccgga tcggccccgc gcatgcccga gctcgaccag caccgacagc
     8101 ggccgcggcg ccccgccggc cacgagtccc tcgcgcagca gttccactcc tcgcgtcgag
     8161 tcgacccagc aggcgaagcc gaacgcctcg tcggcgagct cgcgggcgac ccacccgatc
     8221 gaccccgcgt cgaccagctc gtccgcgagc aggatgcgag gcaccccggc cgagcgcatc
     8281 gcacgagcct ggacgggggt ggctgccgtg acgccccacg cacccgcatc gagctggcgg
     8341 gcccacagct gcggggccat cgacgtcttc ccgtgcggag cgagctcaac accgttcgcc
     8401 cggcagtaag ccgacatcag ctcgaggttg tgctcgagag cgcgctctct cagtaccacg
     8461 accggcggca cgaggtcttc gaacaggttc cacccccgcg atccgacgtc ggcgaccgcg
     8521 agatcggcgc cggccgggaa ccccgtcgtc gtctcctcga cccggacggc gagcaccgct
     8581 cgcaggtcca atctcctgcc gccggtcatc ggcgccccca gcccgcgggg aggtgcgcgg
     8641 cgtgcgcatc gaggtaggcg cccacgagcg catccgccag gttcacgctt cgaacgaggg
     8701 ggtgcacggc cagggccgtc acggccagct cgcgatctcc cgtgaccgca gcctcggctg
     8761 tcatcgcagc gtgcaccgcg acctgtcgga ccagcccgct cacctccacg gggagcgctc
     8821 cctgcgggac aggcgagacg gttcggccgt cgatcgtcgc aggggtctcg acgatcgtgg
     8881 cgtcggggag gtcgtcgacc tgtccggcgt tcgggaggtt caggatcgct cgccggtgcg
     8941 tgtcggtcag catcgcggcc ataacggaga cggcgaagtc gccgtgctcc tcgctcgcac
     9001 gtgccatcga cggtcgcgga tcctcggcgt cggcctcccg tcggtagctc tcgagcaccg
     9061 aggggaggat cgccatcacc tcctgcgcgc gcgtgcgacc ccgcgctcgt tgctcggcca
     9121 gcacctcgtc gtggaagaag aagtactgca tgtactcgct cggcacgtga tgcagcgtgc
     9181 ggaacagctg cacgatgcgc cgctcggcgt cggacgtcag gcgttccaac ggcagggagt
     9241 cgagcagcgt ccacagctcg cgggtgacgt cctcgctcgc gacccgcacc cctctcgtga
     9301 acgtcatgtg gttcgtgccg cacgtgtcga gcgcgatcac atcagcgtcg acgcccagca
     9361 gcctcgcgag gaaccgggct tcgccggcgg tctgatcgca gaggccgagg aacggcagcg
     9421 ccgggccgaa ccggcgcatc gcctcgctca cgacctgcac cgggttcgtg tagtccagga
     9481 ggacggcctc cgaggccgcg agcctcctca tctcttcggc gatctcgagc acgatcggca
     9541 ccgatctgag ggccatggcg aaccccccgg gtccggcggt ctcctgtccg atcacgccgt
     9601 ggtcgatcgc gatgcgctcg tcgaggtgcc gggcctccaa accacccggc cggaaggccg
     9661 cgagcacgac gtcggcgccc tccagcgccg gggaccgatc ggcatgagcc tccacccgaa
     9721 cgcctccagc tcccctcgaa cgcaggatgc tgcccgtcag acgccgttgc aggtcgagca
     9781 cgccggcgtc gatgtcctgc agcaccaggg tcgcgtcccc gaacgcttcg gcatgattgg
     9841 cgaaggcgaa cgcgatcccc ggcatgtagg ccgatccccc gccgatcacg acgacgcgtg
     9901 cgcccatcgg ggccacgcta acagccgggt catgagcggg agcggccgag atgttggcca
     9961 gcgcgagacg ttgggtgacg gagacctgac cgatacccga gagagcactg agagaggaga
    10021 gcacgacgat ggctgaactc gcagtgaacg gagggacccc cgtgcgctcg acgccgtacc
    10081 ctgcgtggcc cgcgcccgac gatgagtacg tcgcggccgt gtccgaggtc gtgcgaggcg
    10141 gcgagtgggg agggttcccc gagcccggcg tgaacgcggc ggcgttcgag gaggccttcg
    10201 ccgcgtacca gggcgctcgc catggggtct tgatgatgaa cggcaccgtg acgatggagg
    10261 tcgcgtgcaa ggcgctcgac gtcggatggg gcgacgaggt gatcctgccg gccctgacgt
    10321 tcgccgccac cgcctacgca ccgatggccg cgggcgccct gcccgtgatc gtggacgtca
    10381 cccccgagac gtggtgcatc gaccccgatc gcgtggaggc ggcgatcacg ccgcgcacgc
    10441 gggcgatcat cccggtgcac ctcggtcacc agatggccga catggaccgg ctgaccgaga
    10501 tcgccgaccg gcacggattg gccgtgatcg aggactgcgc acatgcccac gggcaggagt
    10561 ggcgtggccg cggcgccgga tgcatcggcg acttcggctc gttcagccac caatcgtcga
    10621 agatcctcac ctccggcgag ggcggcacgc tcctgacgaa cgacgacacg ctggcacggc
    10681 gcgcacactc gatcatcgac tgcggacgcg cgaaggacgc cgaggagaag gagttcacgt
    10741 tcggagcgaa ctaccggctc tccgaactga acgccgcgct gctcgtggtc gcgatgcggc
    10801 ggttcccggg acagcaggaa gagcgcgcga ccaacggcct tcgcttcgag cggttggcgg
    10861 ccgacatccc aggcgtgcgg gtcatgcccc gcgacgggcg catcacgcga tggagcttct
    10921 acaactacat cctggcgatc gacccaggtg cgttcggcgg cgccacgaac gagatcgtct
    10981 gcgccgccct cgaggctgag ggcatcccgg ccgaggtgca gtacccgccg atgagccgct
    11041 acgagctgtt ccagccgtcg ctgtcacgtt tacccgtggc cgtcgagcac gccgaccgcc
    11101 tcgacccacg ggggatgtcg ttccccgtcg ccgaggccgc agggctccgc gaatccgtct
    11161 acctcatgga ggcggtgttc cgcgacgggg agcgcggggt gcaggacg
//
LOCUS       DATDBZ010000034         3431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2057138, whole genome shotgun sequence.
ACCESSION   DATDBZ010000034 DATDBZ010000000
VERSION     DATDBZ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2057138"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VIX62_00910"
     CDS             <1..588
                     /locus_tag="VIX62_00910"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5858829.1"
                     /translation="SYVLLGLMVERAAGVSYRDYVTANVFEPAGMQRSGFFRMDHVEP
                     DVAEGTDPIVEDGTVTGWRRNIYSYPPLGSPDGGAHVTAADLVSFHRSLIEGRLLGDD
                     LTASMIEPKEDLRPKGKGMHRTGFGFEFEVTANGEVASYWKEGINVGASGILQHHPAS
                     DVTFVVLSNRESGAWEPIEMIEQMIGGSDPSDDRS"
     gene            complement(585..794)
                     /locus_tag="VIX62_00915"
     CDS             complement(585..794)
                     /locus_tag="VIX62_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858830.1"
                     /translation="MTTIPPGMWQDPEAFEPREVEEIEHRHHADHDHEHGEGCGHESQ
                     PHGDHVDYVHDGHRHWFHHGHWDEH"
     gene            complement(868..2061)
                     /locus_tag="VIX62_00920"
     CDS             complement(868..2061)
                     /locus_tag="VIX62_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858831.1"
                     /translation="MTPSVTPNPNDDHATAAAPISAPGRLAIVGPDGSLVTMRADGSD
                     RVEVSQDAGPSSQPTWSPDGDRLAWVVQVTSESGFSGAIVVAGSRGEQPVVTPTPFLP
                     YYLAWSPTGDRVAFLGAGGDPKAPVEMGVLDLSEDPVRARAVAGGDPFFYFVWTPGGR
                     RVLAHAGFDRLEEIGLDGQATSVTDRPGLFAAPAWSENGATLVYAERAGSGMQRLIAV
                     VNGGRPRVLVEGEGALSFVLRPDGGAVAYQLLGEDDGDFYDRRPTQPGDGVRVVDVRT
                     GKTRKATSIRAMTFWWSPDGERLLALAPEPGSSGTIPFVWQVWEGGRATEAEGRHSPT
                     IEVLRDYAPFFTQYAHSATPWAPDGSAFAFAAEAADGTGRIVVQEVGGRPVAIGEGVY
                     VVWSP"
     gene            2297..>3431
                     /locus_tag="VIX62_00925"
     CDS             2297..>3431
                     /locus_tag="VIX62_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5858832.1"
                     /translation="MLRGRAGGPTVLTQPTVRSSLQPYLPRLTLHWLAEDPKALHRAV
                     DGSVVFVDISGFTKLSEKLARFGRVGAEEMADAINTCFADLLGVAYAEDGMLLKFGGD
                     ALMLLFTDDEPAEHALRAARAAHGMRKRLRTVGKLETTGGKVNLKMSVGVHAGTFDFF
                     LVGGSHRELIVTGPAATQVVTMEGTADAGEIVVSPALASLLPRNCLGDAKGPGEILRF
                     APGGESPSAVWALPEVDDELLRQSVPLATREHLLAGVAEPEHRQASVAFVHFDGTDEA
                     IEREGPEWVADELDRLVRDTQEAVDEFGVCFLASDVDADGGKLILTAGVPRATGEDEE
                     RMLLALRRIVGGERAIPVRIGVNRGSVFSGDIGPPYRRTYTVMG"
BASE COUNT          542 a         1149 c         1184 g          556 t
ORIGIN      
        1 tcatacgtgc tgctcggact catggttgaa cgagccgccg gcgtgtccta ccgggactac
       61 gtcaccgcga acgtgttcga accggccggc atgcaacgtt cggggttctt ccggatggac
      121 cacgtggaac ccgatgtcgc cgaggggacg gatcccatcg tcgaggacgg gaccgtgacc
      181 ggatggcgac gcaacatcta ctcgtatcca ccgctcggct cgcccgacgg aggcgcgcac
      241 gtgacggcag ccgacctggt gtcgttccat cgttcgttga tcgaggggcg gctcctggga
      301 gatgacctca cggcgtcgat gatcgaaccg aaggaggatc tccggccgaa ggggaagggg
      361 atgcatcgca cggggttcgg cttcgagttc gaggtcacgg caaacggcga ggtcgcctcc
      421 tattggaagg aaggcatcaa cgtcggagcc agcggcatcc ttcagcacca cccggcgagc
      481 gatgtgacct tcgtggtgtt gagcaaccgt gagtcgggcg cctgggagcc gatcgagatg
      541 atcgaacaga tgatcggcgg cagtgacccc agcgacgatc gaagctagtg ctcgtcccag
      601 tggccgtgat ggaaccaatg gcggtgcccg tcgtgcacgt agtcgacgtg gtcgccgtgc
      661 ggctgggact cgtgaccgca cccttccccg tgctcatggt cgtggtcggc atgatggcga
      721 tgctcgatct cctcgacctc ccgcggctcg aaggcctcgg ggtcctgcca catgccgggc
      781 gggatcgtcg tcatcggctc ctcctcggat cgggatcgac accggttcct atcacggaac
      841 gtcgtccgca tgggccgcac cgaccggtca cggcgaccag accacgtaga cgccctcgcc
      901 gatcgcgacg ggccggccgc cgacttcctg cacgacgatc cgtcccgtcc cgtctgcggc
      961 ttccgccgcg aaggcgaacg ccgagccgtc gggggcccac ggcgtcgcgc tgtgggcgta
     1021 ctgcgtgaag aacggcgcgt agtcacgcag gacctcgatc gtgggggaat gtcgcccttc
     1081 ggcttcggtc gcgcgccctc cctcccacac ctgccagacg aacgggatcg tccccgacga
     1141 tccgggctcg ggcgcgagcg cgagcagtcg ctcaccgtcc gggctccacc agaacgtcat
     1201 cgctcggatc gaggtggcct tgcgggtctt ccctgtgcgc acgtcgacca cccgtacccc
     1261 gtcgcccggc tgggtagggc gccgatcgta gaaatcaccg tcgtcttcgc ccagcagttg
     1321 gtaggcgacc gctcctccgt cgggtcgcag cacgaacgag agcgccccct ctccctcgac
     1381 cagcacccga ggccgaccgc cgttcacgac cgcgatgagt cgctgcatcc cgcttccggc
     1441 gcgctccgcg tacacgagcg tagcgccgtt ctcgctccat gcgggtgccg cgaacaggcc
     1501 gggtcggtcg gtgacgctgg tggcctggcc gtccaggccg atctcctcga gtcgatcgaa
     1561 cccggcgtgg gcgagcactc ggcgtccgcc gggagtccag acgaagtaga agaacgggtc
     1621 accgcctgcg acggcccggg ccctcacagg gtcttcggag aggtcgagca cccccatctc
     1681 cacgggcgcc ttggggtcgc ctcccgcacc gaggaacgcc acccgatcgc cggtggggct
     1741 ccacgcgagg tagtagggga ggaacggggt gggtgtcacg accggctgct caccacggga
     1801 tccggccacc acgatcgctc cagagaaccc actctcactc gtgacctgga cgacccacgc
     1861 caatcggtcg ccgtcggggg accaggtcgg ttgcgacgag ggccctgcgt cctgtgacac
     1921 ctcgacccga tcggatccgt cggcgcgcat cgtgacgagt gaaccgtcgg ggcccacgat
     1981 cgcgagacgg ccgggcgccg agatcggcgc ggccgcggtt gcgtggtcat cgttcgggtt
     2041 tggggtgacc gaaggggtca cggtggtcga gccgaccgat acctccgccc ggggttcgcc
     2101 gagcgtgaga gagcatccag tcatacttgc tccagagagg agcaccatga gtacgggggc
     2161 gatcgatcca ggccggcgca ccatcgctcg accgtaccgc aggtgggcga tacagcgtca
     2221 ggcctgcacc ccctggaggt tccataccgg tgcatgagtt gtgtacggtg caagcgatga
     2281 gcttcccccg gagcgcgtgc tcagaggccg agccggtggt ccgaccgtgc tgacccagcc
     2341 cacggtcaga agcagtctgc agccctacct gcctcgcctc actctgcact ggctcgccga
     2401 ggatccgaag gccctgcatc gggctgtgga cggctcggtg gtgttcgtgg acatctccgg
     2461 gttcacgaag ctctcggaga agctcgcacg gttcggacgg gtcggcgccg aggagatggc
     2521 cgacgccatc aacacgtgct tcgccgacct tctcggtgtc gcgtacgccg aggacggcat
     2581 gctgctgaag ttcggcggcg acgccctcat gctgttgttc accgacgacg agcccgcgga
     2641 gcacgcgttg cgggctgccc gagcggcgca cggcatgcgc aagcgccttc ggaccgtcgg
     2701 caagctcgag accacgggcg gcaaggtgaa cctcaagatg tcggtcggtg tgcatgccgg
     2761 caccttcgac ttcttcctgg tcggtggctc ccaccgagag ctgatcgtga ccggccccgc
     2821 tgctacgcag gtcgtgacga tggaagggac ggccgacgcc ggcgagatcg tcgtgagtcc
     2881 tgcgctcgcg agcctgcttc ctcgaaactg tttgggcgac gccaaagggc cgggcgagat
     2941 ccttcgcttc gctcccggcg gggaatcgcc atcggcggtg tgggcgttgc ccgaggtcga
     3001 cgacgagctc ttgaggcaga gcgtgcccct cgcgacccgc gagcatctcc tcgccggcgt
     3061 cgccgagccg gagcacaggc aggcctccgt cgcgttcgtc cacttcgatg ggacggacga
     3121 agcgatcgag cgcgaggggc cggagtgggt cgccgacgag ttggatcgtc tcgttcgcga
     3181 cacgcaggag gcggtggacg agttcggtgt ctgcttcctc gcatccgacg tcgacgccga
     3241 cggcgggaag ctgatcctga cggccggggt acccagggcc acgggggaag acgaggagcg
     3301 catgctgctg gcgcttcgcc gcatcgtcgg cggtgaacgg gcgatccccg tgcgcatcgg
     3361 cgtcaaccgc ggcagtgtgt tctcaggtga catcggtccg ccgtaccgac gcacctacac
     3421 cgtgatgggt g
//
LOCUS       DATDBZ010000035         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_627301, whole genome shotgun sequence.
ACCESSION   DATDBZ010000035 DATDBZ010000000
VERSION     DATDBZ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627301"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VIX62_00930"
     CDS             complement(<1..554)
                     /locus_tag="VIX62_00930"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEY5858833.1"
                     /translation="MQIAFVLYDRFTALDIIGPFNVLAYVPGVEARFVAETLEPVTAD
                     VGRPVIVPDLTFAECDRPDIVVVPGGPGSTRIAELTALTDWLRRVHPTTTYTTSVCTG
                     AYALAGAGLLDGLRATTHWSALDGLSAYGAEPVAERVVDEGRIITGAGVSAGIDMALT
                     LVGRHWGDTVAQAIQLGIEYDPQPP"
     gene            complement(581..1429)
                     /locus_tag="VIX62_00935"
     CDS             complement(581..1429)
                     /locus_tag="VIX62_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858834.1"
                     /translation="MKLLQRTISIVLTVVGAALLAMGTVLWWADGHLFDTATVVAQTD
                     DVLTSPDVQVLLTARITDHVMVYVGDQTFRPQVEMIVEEAVADPRVRALVNDGVRQTH
                     LALVSGDAEQVALRLEALSREIRQTVVATVPELAGRIPDTDVLTFDLITRSDLPDVWR
                     LAERFHEAAAMFLSIGAVLVGVGLVIGPARWLRLLVGGLTFAAFGVVCFVALRAAITV
                     VEDRITDPLAAAAAGDIFDAFFSSLDTRAIVMVIGGLLAAMFGVAIRLMRPEYELHTR
                     RASPFR"
     gene            complement(1443..2117)
                     /locus_tag="VIX62_00940"
     CDS             complement(1443..2117)
                     /locus_tag="VIX62_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5858835.1"
                     /translation="MSVPEQPVADTGADTDTDAGADAGPDPSVAGGRLDRQIDFLLEA
                     DRLKQIERMTKILGGSRRENSAEHSWHLALFALVLCEHADDPLDLARAITMLLLHDLV
                     EIDAGDTFAYDDAGYETKVAREEAAADRLFGLLPEAQGAQLRALWDDFEAGTSPEARF
                     ANAVDRLQPLLLNHANQGGPWREHGISRERIVARNSPIAAGSERLWALAQDRIDDVQA
                     LGLLPD"
     gene            complement(2114..2320)
                     /locus_tag="VIX62_00945"
     CDS             complement(2114..2320)
                     /locus_tag="VIX62_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858836.1"
                     /translation="MFLGDDLLTYLVMAMGGALCVGNVFAVARPPAQHREGELARAPV
                     GRSLVMAAIGFLAFAWAMASLVAG"
     gene            2381..2929
                     /locus_tag="VIX62_00950"
     CDS             2381..2929
                     /locus_tag="VIX62_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5858837.1"
                     /translation="MGFHHVALATRDTAATHDFYTRVMEFELVKVVTAPTPGEHGGWS
                     KHFFYATGSSSDNGMIAFWEIHDEQIGDQFPVDINAAAGLPWWVNHIAFDAPSREDLD
                     RHRRRWQECGFRVLEVDHEFCVSIYIRDPNGNMVEFCQTVRDFTPAERSRAHDLLLDE
                     HPPLEKDFSAQVWEPVATVSSP"
BASE COUNT          566 a         1074 c         1129 g          495 t
ORIGIN      
        1 ggcggttgcg ggtcgtattc gatgccgagc tggatcgcct gtgcgaccgt gtcgccccag
       61 tggcggccga cgagcgtcaa cgccatgtcg atcccggctg agacgccggc tcccgtgatg
      121 atccggccct cgtcgaccac ccgctcggcc accggctcag ctccgtatgc ggacagccca
      181 tcgagcgcgc tccagtgggt cgtggcccgc aatccgtcca gtaggccggc gccggcgagt
      241 gcgtacgcgc cggtgcagac cgaggtggtg taggtggtgg tcgggtggac ccgacgcagc
      301 caatcggtca gggcggtgag ctcggcgatc cgggtggaac cggggccacc cggcaccacg
      361 acgatgtcgg ggcgatcgca ctcggcgaag gtgaggtccg gcacgatcac cggacggccg
      421 acgtcggcgg tgaccggctc cagcgtctcg gccacgaacc gggcctcgac gcccggcaca
      481 taggcgagca cgttgaacgg tccgatgatg tcgagcgccg tgaaacggtc gtagaggacg
      541 aaggcgatct gcacgagggt gctcctcctg atcgggcgag tcagcgaaac ggtgacgcgc
      601 gacgggtgtg cagttcatac tccgggcgca tgaggcggat cgccaccccg aacatcgccg
      661 ccagcagccc gccgatcacc atcacgatgg ctcgagtgtc gaggctggag aaaaaggcat
      721 cgaagatgtc gccggccgcc gcggctgcca gtggatcggt gatgcggtcc tcgaccacgg
      781 tgatcgcggc gcgcaaggcg acgaagcaca cgacgccgaa cgccgcgaac gtgagcccgc
      841 cgacgagcag gcggagccag cgggcgggtc cgatcacgag gcccacgccg accagcactg
      901 cgccgatgct caggaacatc gcggcggctt cgtggaatcg ctcggcgagg cgccacacgt
      961 cgggaaggtc ggagcgggtg atgagatcga aggtcaacac atcggtatcg gggatacgac
     1021 cagcgagttc gggcaccgtg gcgacgacgg tctggcggat ctcacggctg agcgcttcga
     1081 ggcggagcgc cacctgctcg gcgtcgccgg aaacgagcgc caggtgcgtc tgacgaaccc
     1141 cgtcgttgac gagggcgcgc acccgaggat cggcaactgc ttcctccacg atcatctcga
     1201 cctgcggccg gaacgtctgg tcgccgacgt agaccatgac gtgatcggtg atgcgagccg
     1261 tgaggagcac ctgcacatcc gggctcgtga gcacgtcgtc cgtctgcgcc accacggtgg
     1321 cggtgtcgaa caggtgcccg tcggcccacc agaggaccgt tcccatggcg aggagggcag
     1381 caccgaccac cgtcagcacg atcgagatcg tgcgctggag cagcttcatc ggggaaggcc
     1441 catcagtcgg gaagcaatcc caatgcctgc acgtcgtcga tccggtcctg ggcgagcgcc
     1501 cagagccgct ccgagcccgc ggcgatcggc gagttccggg ccacgatccg ctcgcggctg
     1561 atgccgtgct cccgccaggg accgccctgg ttggcgtggt tcaacagcag cggttgcaac
     1621 cggtcgacgg cgttcgcaaa ccgcgcctcg ggagacgttc cggcttcgaa gtcgtcccag
     1681 agcgcccgca gttgcgcgcc ctgagcctcg ggtagcagac cgaaaagacg gtcggcagca
     1741 gcctcctcgc gggccacctt ggtctcgtag cccgcatcgt cgtaggcgaa cgtgtcgccg
     1801 gcatcgatct cgacgagatc gtgcaggagc agcatcgtga tggcgcgagc caggtcgagc
     1861 gggtcgtcgg catgctcgca gagcacgagc gcgaacaacg cgagatgcca ggagtgttcc
     1921 gcgctgttct cacgacggga gccgccgagg atcttcgtca tccgctcgat ctgcttgagg
     1981 cgatcagctt cgaggaggaa gtcgatctgt cggtcgagac ggcccccagc cacggacggg
     2041 tcgggaccgg catcggcacc ggcgtcggtg tcggtgtcgg caccggtatc ggcgacgggc
     2101 tgctccggca cgctcatccg gccacgagcg atgccatcgc ccatgcgaac gcgaggaacc
     2161 cgatcgcagc catgacgaga ctgcggccca ccggtgcgcg cgccaactcg ccctcccggt
     2221 gctgtgcagg tggacgggcg acggcgaaca cgttgccgac gcacagcgcg ccgcccatcg
     2281 ccatcacgag atacgtcaac aggtcgtcac cgaggaacac gggaaggatc gtacggcgct
     2341 cagcgtcgcc cgtgcccggc aggcgcgcga cacttgacgg atgggattcc atcacgtcgc
     2401 gctcgccact cgtgacaccg ccgccaccca cgatttctac acgcgggtga tggaattcga
     2461 gctcgtcaag gtcgtcaccg cacctacccc gggcgaacac ggggggtggt cgaagcactt
     2521 cttctacgcc accgggtcct cgagcgacaa cggcatgatc gccttctggg agatccacga
     2581 cgaacagatc ggcgaccagt tccccgtcga catcaacgcc gccgccgggc tgccgtggtg
     2641 ggtgaaccac atcgcattcg acgctccgtc gcgggaggat ctcgatcgcc atcgtcggcg
     2701 ctggcaggag tgtggctttc gcgtgctcga agtcgaccac gagttctgcg tctcgatcta
     2761 catccgcgat ccgaacggca acatggtcga gttctgccaa acggtccgcg acttcacccc
     2821 ggccgagcgc agccgcgccc acgacctgct cctggacgag cacccgccac tggagaagga
     2881 cttctccgcc caggtctggg agcccgttgc cactgtgtcc agcccctgac gccgtcggac
     2941 aaccggccgg aagttcgcct atagggagaa tagaccgcca cagccctgta accagggcaa
     3001 accgacgggt ggcgtcccgg agggactctt ttccgccatg gcgccccact ggggctacgg
     3061 aatctttgtg atgtctgggg cgtgggtgtc atcgacgggt gaggcgtgcg acggcggtgg
     3121 ggaccacgat ggcgtgtgtg cactgggttg cgcatagcgc aactgggcgc gcaatcgctg
     3181 gtcgttcagc cgggcgtcga tggcgtgtgt gcactgggtt gcggatggcg caaaatcgct
     3241 tgtcgttcag gtcgggcgtc gatg
//
LOCUS       DATDBZ010000036         7495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1854873, whole genome shotgun sequence.
ACCESSION   DATDBZ010000036 DATDBZ010000000
VERSION     DATDBZ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854873"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..940
                     /locus_tag="VIX62_00955"
     CDS             152..940
                     /locus_tag="VIX62_00955"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5858838.1"
                     /translation="MHVWEAGPDDADAPTIFAVHGLGGSGRYWQGLANRTAGTHRLIA
                     PDLAGFGRSEEPTLDADRALHLDDLDAVIEATAPSSPVWVVGHSLGGVLAALWAGDRH
                     DHVAGLALAAAPFPTGEAMDFRSRADLQASPVRRAMGRGVRTIWPVIAVPVGLARGYP
                     PATVIDFGRQSVRSRAWTLWSLWSDPSLVEEIQRVGSLDGTLPALLAHARDDRTVPIT
                     AHGAWTHLLPHAEEHVIESGGHQFLLRTGFEPVTSWLRTSSGTS"
     gene            complement(915..3614)
                     /locus_tag="VIX62_00960"
     CDS             complement(915..3614)
                     /locus_tag="VIX62_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HEY5858839.1"
                     /translation="MRRNLRRISLFSLLAIVIGLAGMVPAGATLSLLPDDTWGTNNQV
                     SVILRVGGTIYLGGEFTALEADNGATMARNHLAAIDAATGEPTSFNPNVSGSVFGLAS
                     SPDGSRLYAVGNFTTVGGQARKMVAAFDLPSGALSSWKPAGSPNTVVRAVAVTSDKVY
                     IGGSFNTLGSTPRAKLAALSPVDGSISTTWMPTADGLVRDIVVQGTRVFIGGNFVQVN
                     GTDQRRFAGLDVSSGASACSTCYHPTYPILDIDAGPNRIYLAGGGGGGKAVALTTSTG
                     AKLWEKKTDGNVQAVSTLNGVPYFGGHFFKYTSIPVNQLVRTDPVTGVLDQSWLPNVT
                     AGFLGVFAVRGFNANLYVGGDFTRVAQTKQLNFAQWTDSAVSASIDVGVSLADAPDPV
                     DAGSSLTYTATVTNAGPDAAAGVTLTDNLPASATFVSASPSCTYSSGTHSVSCDLGTI
                     AAGGSTAPTITVTPGSAGSLNNTVNVTAVGNDPNAANNSSSASTTVNAVAGADLNLTM
                     SPANAAKIDQGSMFSYVLTVLNQGPDSDPSVTVTDTLPGNVTPAGAATTTAGSCSGTT
                     TVTCALGAMNANGVATITIPVTAPAQPQTVVNTASVDGLANDPDHADDEVVRFNAVKD
                     PALGGDTTAPSRTATQMFDVDADGFVDQVVVTFSENLAVCPAPCTSGWVLTNVPSGSS
                     LQSVSIAGNQAILSIGNEQNQPDTAVGLFRVQLATPNQIQDAAGNRSVFSAVVPTDKA
                     SPVAVGFRKGHNSSGVCSGAPSNGGVAEPCDELTSEWSEALLPSSIPSTANLTITDPA
                     GSGNDLLTVSDFIQGSMDIKSNNYVTLDTGTASWASSLLLLQGGAAQDTVTVRIAGSC
                     TGTGCASLASVGDVTIVYVPAASVKDVAGNPAAGSFTKFQKMF"
     gene            3806..4222
                     /locus_tag="VIX62_00965"
     CDS             3806..4222
                     /locus_tag="VIX62_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858840.1"
                     /translation="MDARSFDFWLGTWRCAWDGGEGTNAVTRTLDEHVILERFTAAGA
                     EAFEGMSVSVFDRAQGWRQTWVDSNGSYWHFVGGHDEAGDLCLTTPRPVDAERHVKRM
                     VFSGITPEAFAWRWESSPDAVTWTQRWAIDYRRASP"
     gene            complement(4238..5599)
                     /locus_tag="VIX62_00970"
     CDS             complement(4238..5599)
                     /locus_tag="VIX62_00970"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5858841.1"
                     /translation="MDQRTSDADPPEPDARNTGRSIPRLFRTFLEQAPEIDQFTLRFV
                     EVDTEHAFQRSYQRDTLPYVRLAFILGVAGWALFGFLARRLVPEGGDVDTILRYGVAV
                     PLTLMALVCTYTAWWGRRWQLSISLVLVANALIWSVDRVALPASAPTDWGYAGMMVIL
                     AFIYVLSRLQFAYAAIVGAVSIVIYDVLTIAFASDTNTQFLVANYFLVTFAFVGMAAS
                     YGLDRSARLLFLRERQLDAARVRADELLHNTLPAAIVERLKARGSEPGVELLADGHEQ
                     VTVVFADLVSFTEHAERIAPAPLVAVLDEVFTMFDDLAQRVGMEKIKTVGDAYMAVAG
                     APEPRPDHAQAAAEMALQIRASMADARWPTGESIQVRVGIATGPVVAGVIGRHRFAYD
                     LWGDTVNLASRLESSGTPGEILVSETTAALLEGRYELGTRRVMELKGKGATPAVPLIG
                     PGS"
     gene            complement(5633..6313)
                     /locus_tag="VIX62_00975"
     CDS             complement(5633..6313)
                     /locus_tag="VIX62_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858842.1"
                     /translation="MGTARPLEVVAPTDEPIVGWRAWHLAPGAPGLLPAGAGVGSDTW
                     QPRAATTARCGLNRLLSIGRPVHESPGERCRCGIYAHRSLEDFARDTVPAYPPPSVVG
                     TVALWGRVIEHERGWRAAHAYPARLRLVCVLCAWVEPGPGTPAVVHEFDGHASVLCRD
                     HREGIELPDGRRTTPTGIDPALLQARLLDAYAVDLLPADRLDPLLDRPSAAPPPPFFP
                     VIRPVRLP"
     gene            6379..6870
                     /locus_tag="VIX62_00980"
     CDS             6379..6870
                     /locus_tag="VIX62_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY5858843.1"
                     /translation="MAPVDSPTPATEALEGSGLAFEVVRFEPPSSAQESADRQGIDLA
                     SLLRTIVVRRGADDFVFVLVPGGRQIAWPKLRSHLGVKRISLPDAEEAKAVTGYERGA
                     ITPFGSIHPWPVIADATVASLGRVAIGGGARGVNLHLDANDLIGATHAQVADVTVEAP
                     VPN"
     gene            complement(6881..>7495)
                     /locus_tag="VIX62_00985"
     CDS             complement(6881..>7495)
                     /locus_tag="VIX62_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5858844.1"
                     /translation="GDTVVLYTDGLVERRDESLDEGLERLRQTAEHVAGRDADRDRLG
                     DALAAELIDEGTSVDDVAILTVTLRPSFHHIIERDPGALAPARAALRTWLDGHQVPGG
                     IVEELVLASSEALANAIEHAHPTPEGSIEFRGWIDGARVVVHVADDGRWTERDPDPVR
                     GNGLMIMRTLMDEVVVDREADGTRIELIRSLTRPPMSVGGPVGS"
BASE COUNT         1142 a         2562 c         2673 g         1118 t
ORIGIN      
        1 cccacggtag ggtcgcacga tgggcgttcg tcggtacatc accttgccgc cgcgcggcct
       61 cggcgtgcac gacgcccccc tgcgggtcgg ccgctgagtg gccgatcccg cccgcctgcg
      121 cagcttgacg ctcgaccgac cgtggggaca catgcacgtg tgggaagccg gccccgacga
      181 cgcggatgcg cccacgatct tcgccgtcca tggcctgggc gggtcgggcc ggtactggca
      241 agggttggcc aaccggacgg ccggcaccca ccggctcatc gcgcccgacc tcgccgggtt
      301 cggccgctcc gaggaaccga cgctcgacgc cgatcgcgca ctgcacctgg acgatctcga
      361 cgccgtgatc gaggccaccg ctccttcctc gccggtgtgg gtggtcggac attcactggg
      421 cggcgtcctt gccgcactgt gggcgggcga ccggcacgat catgtggcgg gtctggcgct
      481 cgccgccgcg cccttcccaa cgggcgaggc gatggacttc cgatcccggg ctgatctgca
      541 ggcctcaccg gtgcgacgag cgatggggcg gggcgtccgc acgatctggc cggtgatcgc
      601 ggtgccggtg ggcttggcgc gcggctaccc gcccgccacc gtgatcgact tcggccggca
      661 atcggtccgc agccgggcgt ggaccttgtg gtcgctgtgg tccgacccgt cgttggtcga
      721 ggagatccag cgggtcggtt cgctcgatgg gacgctcccg gcgctgctcg cccacgcgcg
      781 cgacgaccgg acggtgccga tcaccgccca cggagcctgg acgcacctgc tcccgcacgc
      841 ggaggaacac gtgatcgaga gcggcggcca ccagttcctg ctccgcaccg gcttcgagcc
      901 ggtgacgagc tggctcagaa catcttctgg aacttcgtga agctgcccgc cgccgggttg
      961 ccagcgacgt ccttgaccga ggccgccggc acgtacacga tcgtgacgtc gccgaccgag
     1021 gccaacgatg cgcagcccgt cccggtgcac gaacccgcga tgcggacggt cacggtgtcc
     1081 tgcgccgctc ccccttggag caacagcaac gacgatgccc acgacgccgt accggtgtcg
     1141 agcgtgacgt agttgttgga cttgatgtcc atcgaaccct ggatgaagtc gctgacggtc
     1201 agcaggtcgt tgcccgagcc cgccgggtcc gtgatcgtca ggttcgccgt cgacgggatc
     1261 gacgacggca acagcgcctc gctccactcc gacgtcaact catcgcacgg ctcggccacg
     1321 ccgccgttcg acggcgcacc gctgcacacg ccgctggagt tgtgaccctt gcggaacccg
     1381 accgcgacag ggctcgcctt gtcggtgggc accaccgccg agaagaccga gcggttgccg
     1441 gctgcgtcct ggatctggtt cggggtggcc agttgcacgc ggaacaaccc cacggcggtg
     1501 tcgggctggt tctgctcgtt gccgatcgac aggatggcct ggttcccggc gatcgagacc
     1561 gactgcaggg agctgcccga gggcacgttg gtcagcaccc agccggacgt gcacggagcg
     1621 ggacacacgg cgaggttctc gctgaacgtg acgaccacct gatcgacgaa cccgtccgca
     1681 tcgacgtcga acatctgcgt cgcggtcctg gacggggccg tggtgtcgcc accgagggcc
     1741 gggtccttca cggcgttgaa gcggaccacc tcgtcatccg cgtggtccgg gtcgttcgcg
     1801 aggccatcga cggacgcggt gttgaccacc gtctgcggct gcgccggggc ggtcacgggg
     1861 atcgtgatcg tggccacacc gttcgcgttc atggcgccga gcgcacaggt gacggtggtg
     1921 gtcccggagc acgaacccgc cgtcgtggtg gccgcccccg ccggcgtgac gttgcccggc
     1981 agggtgtcgg tgacggtcac gctcgggtcg ctgtccgggc cctggttcag cacggtgagc
     2041 acgtaggaga acatgctgcc ctggtcgatc ttcgcggcgt tggccggcga catcgtcagg
     2101 ttgaggtcgg cgcctgcgac ggcgttcacc gtggtgctgg cgctcgagct gttgttcgcc
     2161 gcgttcggat cgttgccgac ggccgtgacg ttcacggtgt tgttcaacga gccggcgctg
     2221 cccggcgtca cggtgatcgt gggggccgtg gatccgccgg ccgcgatcgt gccgaggtcg
     2281 catgagaccg agtgcgtgcc cgacgagtag gtgcagctcg gggacgcgct cacgaacgtc
     2341 gctgaggccg ggaggttgtc ggtgagggtc acgccggcgg cagcgtcggg cccggcgttc
     2401 gtcacggtcg cggtgtaggt gagggacgac cccgcgtcga cgggatcggg cgcgtcggcc
     2461 agggacacac ccacgtcgat cgaggcggag accgcgctgt cggtccactg cgcgaagttc
     2521 agctgcttcg tctgcgccac gcgcgtgaag tccccgccca cgtacaggtt ggcgttgaag
     2581 cctcgcaccg cgaacacgcc gaggaacccg gccgtcacgt tcgggagcca cgactggtcg
     2641 agcacaccgg tgaccgggtc ggtcctgacg agctggttca ccgggatcga cgtgtacttg
     2701 aagaagtgcc cgccgaagta gggcacgccg ttcagcgtgc tgaccgcctg cacgttgccg
     2761 tcggtcttct tctcccagag cttcgcgccc gtggaggtgg tcagcgccac cgccttgcca
     2821 ccaccgccac cgccggcgag gtatatccgg ttcggaccgg cgtcgatgtc gaggatcggg
     2881 tacgtcgggt ggtaacacgt ggagcatgct gaagcgccgc tgctgacgtc gaggccggcg
     2941 aaccgccgct ggtcggtgcc gttcacctgg acgaagttgc cgccgatgaa cacccgcgtc
     3001 ccctgcacca cgatgtcgcg caccaggccg tcggcggtgg gcatccacgt ggtcgagatc
     3061 gaaccgtcga ccgggctcaa cgccgcgagc ttggcgcggg gcgtcgaccc cagcgtgttg
     3121 aacgagccgc cgatgtagac cttgtccgag gtcacggcga ccgcacgcac gacggtgttg
     3181 ggcgatccgg ccggcttcca gctggagagg gcgccggacg ggaggtcgaa ggccgcgacc
     3241 atcttgcggg cctgtccgcc caccgtggtg aagttgccga ccgcgtagag acgggaaccg
     3301 tcgggcgagg aggcgaggcc gaagaccgag ccgctgacgt tggggttgaa ggaggtgggt
     3361 tcacccgtgg cggcgtcgat cgcggcgagg tggttgcggg ccatcgtggc gccgttgtcc
     3421 gcctcgagcg cggtgaactc cccgcccagg tagatcgtgc cacccacacg caggatgacg
     3481 ctgacctggt tgttggtgcc ccacgtgtcg tcgggcagga gcgacagggt tgcgccggcg
     3541 gggaccatgc cggccagacc gatgacgatg gcgagcagcg agaagagcga gatccttcga
     3601 aggttgcgcc tcatgatgtt ccgccccctc ccagggcgaa tgccttccag ggagaatccc
     3661 gagatactac tccgaacgca ctagggagca acccccacgc cgataatcga cccgttcgcc
     3721 ctagtgctga tgcgccgaga aggcatctcc gaagcgggca acgaaccgga agcatctcgc
     3781 gacggacgtc catagccttc ctgggatgga tgcgcgcagt ttcgatttct ggctcgggac
     3841 ctggcgatgt gcctgggacg gcggcgaggg caccaacgcg gtcacgcgca ccttggacga
     3901 gcacgtcatc ctcgaacggt tcacggcggc cggagccgaa gcgttcgagg gcatgagcgt
     3961 gtcggtgttc gaccgcgcgc aaggatggcg gcagacgtgg gtcgactcga acgggagtta
     4021 ctggcacttc gtgggcggac acgatgaagc gggtgacctc tgcctcacca ctccgagacc
     4081 ggtcgatgcc gaacgtcacg tcaagcgcat ggtgttcagc ggcatcacgc ccgaggcgtt
     4141 cgcctggcgg tgggaatcct caccggacgc ggtcacctgg acgcagcgat gggcgatcga
     4201 ctaccgccga gcttctccat gaagagagcg tgagcgctca cgaccccggt ccgatcagtg
     4261 gaacggcagg tgtcgcgccc ttgcccttga gctccatcac ccggcgggtg cccagctcat
     4321 accgaccttc caacagcgcc gcggtggtct cggatacgag gatctccccg ggggtcccgg
     4381 acgattccag ccggctcgcc aggttcacgg tgtcgcccca caggtcgtag gcgaaccgat
     4441 gccggccgat caccccggcc accaccgggc cggtcgcgat gccgacacgg acctggatcg
     4501 attcaccggt cggccaccgg gcatcggcca tgctcgcgcg gatctgcaac gccatctcgg
     4561 cggcggcctg cgcgtgatcg ggacgcggct cgggcgcccc cgccaccgcc atgtaggcgt
     4621 cgcccaccgt cttgatcttc tccatcccca cgcgttgcgc gagatcgtcg aacatcgtga
     4681 agacctcgtc gaggaccgcc accaacggcg ccggggcgat gcgttcggcg tgctcggtga
     4741 acgagaccag gtcggcgaac accaccgtca cctgttcgtg accgtcggcg aggagctcga
     4801 cgccgggttc ggacccgcgc gccttcagcc gctcgacgat cgcggccggc agggtgttgt
     4861 gcagcaactc gtcggcgcga acgcgcgcgg cgtccaactg acgctcgcgc aagaacagca
     4921 ggcgggcgga ccggtcgaga ccgtaggagg ccgccatccc gacgaacgcg aacgtcacca
     4981 agaagtagtt ggcgaccagg aactgggtgt tggtgtcgct ggcgaacgcg atcgtgagca
     5041 cgtcgtagat cacgatcgac accgcgccca cgatcgcggc gtacgcgaac tgcaggcgtg
     5101 acagcacgta gatgaacgcc aggatcacca tcatccccgc gtacccccag tcggtgggcg
     5161 cgctggcggg caacgccacg cgatcgaccg accagatcag ggcgttcgcc accagcacga
     5221 gcgagatgga cagctgccaa cgccggcccc accaggcggt gtacgtgcac accagcgcca
     5281 tcagggtgag cgggaccgcc acgccgtagc ggaggatggt gtcgacatct ccgccttcgg
     5341 gcacgagccg ccgggcgagg aaaccgaaga gggcccatcc cgccaccccg aggatgaagg
     5401 ccagccggac gtacgggagg gtgtcgcgct ggtagctcct ctggaaggcg tgttcggtgt
     5461 cgacctcgac gaaccggagc gtgaactgat cgatctcggg cgcctgctcc aggaaggtcc
     5521 ggaacagccg agggatcgag cgcccggtgt tccgggcgtc gggctcaggc gggtcggcgt
     5581 cggacgtgcg ctgatccatc cggctggatg ttcgcatgca ggaccgccct cgtcagggca
     5641 ggcgaaccgg ccggatcacg gggaagaacg gcggcggcgg cgcggcggag ggccggtcca
     5701 gcagcgggtc gagccggtcg gcgggcagca ggtcgaccgc ataggcatcg agcagccggg
     5761 cctggagcag cgcggggtcg atgccggtgg gggtggtgcg ccggccgtct gggagctcga
     5821 tcccctcccg atggtcccga cacaggaccg aggcatgacc gtcgaactcg tgcacgacgg
     5881 ccggggtgcc cgggccgggc tcgacccagg cacagaggac gcacaccagt cgcaggcggg
     5941 cggggtaggc gtgagcggcg cgccagccac gttcgtgttc gatcacgcgc ccccacaacg
     6001 ccaccgttcc caccaccgag ggtggcgggt acgccggcac cgtgtcgcgg gcgaagtcct
     6061 cgagggaacg gtgcgcgtag atgccgcatc ggcaccgctc gcccggactc tcgtggacgg
     6121 ggcgtccgat cgagagcaac cggttgagac cgcaccgagc cgtggtggcg gcgcgtggct
     6181 gccaggtgtc cgaccccacg ccggcgccgg ccgggagcag ccccggcgcc ccgggcgcca
     6241 gatgccacgc tcgccagccg acgatgggct cgtcggtcgg cgcgacgacc tcgaggggcc
     6301 gggctgtacc catcgttcga gggtgcgccc caccggcggg aaccgcaagg gagccgccgg
     6361 acgctaggct gcgccgcgat ggctcccgtc gacagcccca cgcccgccac ggaggcgctc
     6421 gaaggctcag gcctcgcctt cgaggtggtg cggtttgagc cgccgtcgag tgcacaggag
     6481 agtgcggacc gccaggggat cgacctcgcc tcgttgctgc gcacgatcgt cgtccggcgc
     6541 ggcgccgacg acttcgtctt cgtgctggtg ccgggaggac ggcagatcgc ctggccgaaa
     6601 ctccggtcgc atctgggggt caagcgcatc tcgctccccg acgccgagga ggccaaggcc
     6661 gtgaccggct acgaacgggg cgccatcacg cccttcggct ccatccaccc gtggccggtg
     6721 atcgccgatg cgaccgtggc ttcgctcggc cgcgtcgcca tcggcggcgg cgcccgcggg
     6781 gtgaacctgc atctcgacgc gaacgatctg atcggcgcga cccacgccca ggtcgcggat
     6841 gtcacggtcg aggcgcccgt gccgaactga cccccccgcc tcaggaaccg accggaccgc
     6901 ccaccgacat agggggtctg gtgagcgatc ggatcagttc gatgcgggtg ccgtcagcct
     6961 cgcggtcgac caccacctcg tccatcaggg tccgcatgat catcaggccg ttgccccgga
     7021 cggggtccgg atcgcgttcg gtccaccgtc cgtcgtccgc cacgtgcacc acgacccggg
     7081 cgccgtcgat ccagccacgg aactcgatgg agccttcggg cgtggggtgg gcatgctcga
     7141 tcgcgttcgc gagggcctcg ctgctcgcca ggaccagttc ctccacgatg cccccgggga
     7201 cctggtggcc gtccaaccag gtccgcagcg cggcgcgcgc gggagcgagt gcaccggggt
     7261 cgcgctcgat gatgtggtgg aagctcggcc ggagcgtcac ggtgaggatg gcgacgtcgt
     7321 cgaccgacgt gccctcgtcg atcagttcgg cggccagggc atcgccgagg cggtcgcggt
     7381 ccgcgtcccg tcccgccacg tgctccgccg tctggcgcag acgttcgagg ccttcgtcca
     7441 gggactcgtc gcgacgctcc accaagccgt cggtgtagag gaccaccgtg tcgcc
//
LOCUS       DATDBZ010000037         3689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2059746, whole genome shotgun sequence.
ACCESSION   DATDBZ010000037 DATDBZ010000000
VERSION     DATDBZ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059746"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VIX62_00990"
     CDS             <1..1135
                     /locus_tag="VIX62_00990"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEY5858845.1"
                     /translation="MGSFSRPSVIGRAHGALIAINGDFGLLDGYPIHPFLMDGTLMGR
                     GVQNGTSFAVAHDETAAFVGPPRPVVHGLLETGNVGFPVSGWNSHRPAGGTISAFTRY
                     GGGIHRPPDNGCAVRLDADGPLGWAALEAGVSRRYTVDVVRCVSPPRRLGSQEVVLAS
                     RRWGPGADAIEAMQPGDRVRLSWSVGWPRVLDALGGMPRLVDDGRSVAPRCRSSFCRR
                     NPRTGIGVTSDGRLLLVVVDGRSRRSVGMTLRRFARTFLDLGAVEAVNLDGGGSSVMW
                     IRGRGIVNSPSDPGGERAVVNALMILPGADAGEEPMTGIDVDGVIGRASIGGAWSATA
                     GSLAASDAGSTGGLAAAWLDGALGPEPVSPALRRLGLSFSVGR"
     gene            complement(1263..1550)
                     /locus_tag="VIX62_00995"
     CDS             complement(1263..1550)
                     /locus_tag="VIX62_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858846.1"
                     /translation="MLVLPDEGSIDEGSEDWGRLGREVRLARIDVPASDFSYRYRLTA
                     SIAMAEIARQIGIRGREVRAASRRGLTYESVLRTLSPEHAEEITWDALSLY"
     gene            complement(2302..2982)
                     /locus_tag="VIX62_01000"
     CDS             complement(2302..2982)
                     /locus_tag="VIX62_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858847.1"
                     /translation="MARTKHTDPPRIRAERRAKDPRASRGSGDISDAHARARLFKELG
                     ITLVPQGIATDEDRSLPRITTTRPRVGFAHPLDRREIRRMLTFVGPIGTYGIKTVELR
                     HRPGEQAGTISLARYRSPDSILLFEQPTPPWVLPGRLNEATAVRLHRAGALIDLAEMT
                     TVIDWPDDSLSLFMAFDGLLHEIGHHVIQQYEGKRNIRKKRTADHERRADAFALACRK
                     AWTDEAGR"
     gene            complement(3176..>3689)
                     /locus_tag="VIX62_01005"
     CDS             complement(3176..>3689)
                     /locus_tag="VIX62_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5858848.1"
                     /translation="DHVIANAAGCGAHMQTYGELADGTELPVRDVIAFLHDEGLGAVE
                     LAPADVERVAYHDACHALRAQGIHAQPRALLGAIPGLQVVEIPHGDRCCGAAGLYNVT
                     EPELAERLQRDKTEAVRSTGARVIASANPGCTMQLRAGLAAIGVDARVVHPVELLDAA
                     IEAGSERRRA"
BASE COUNT          539 a         1201 c         1319 g          630 t
ORIGIN      
        1 gatgggctcg ttctcacggc cgagcgtgat cggccgggcg cacggcgcgt tgatcgcgat
       61 caacggggac ttcgggctcc tggacgggta tccgatccac ccgttcctga tggacgggac
      121 cctgatgggc cggggcgtgc agaacgggac gagcttcgcg gtcgcccacg acgagaccgc
      181 ggcgttcgtc gggccgccgc gaccggtcgt ccacgggctc ctcgagacgg ggaacgtcgg
      241 ctttccggtc tccgggtgga actcacatcg tcccgcgggc ggcacgatct cggcgttcac
      301 gcggtacggg ggaggcatcc accggcctcc cgacaacggg tgcgcggtga ggctcgatgc
      361 ggacgggccg ttgggttggg cggcgctcga agcaggggtg tccaggcgct acacggtcga
      421 tgtcgttcga tgcgtctctc cgcccaggcg gttgggatct caggaggtcg tgctcgcgtc
      481 gaggcgctgg gggcccggcg ccgacgcgat cgaggcgatg cagcccggcg atcgcgtccg
      541 cctctcctgg tcggtcgggt ggccgagggt gctcgacgcg ctcggcggca tgccgcggct
      601 cgtcgacgac ggcaggtccg tcgccccgag gtgccggtcg tcgttctgcc ggcgcaatcc
      661 ccgaacgggc atcggggtca cgagcgatgg caggctgctg ctggtcgtcg tcgacggccg
      721 gtcgcgacgg tcggtcggca tgaccctgcg gcggttcgcc cgcaccttcc tcgacctcgg
      781 agccgtcgag gccgtgaacc tcgacggcgg cggcagctcg gtgatgtgga tcaggggccg
      841 agggatcgtg aactcgccct ccgaccccgg cggcgagcgg gccgtcgtga atgcgttgat
      901 gatcctgccc ggggccgacg cgggcgagga accgatgacg ggtatcgacg tcgacggggt
      961 gatcggccgg gcgtcgatcg ggggagcgtg gtccgcgacg gcggggtcgc tggccgcgag
     1021 cgatgccggg tcgaccggag gactcgccgc cgcatggctc gatggcgcgc tgggcccgga
     1081 gccggtgtcg ccggcccttc ggcggctcgg gctgtcgttc agcgtcggac gctaaggttg
     1141 ctcacgccaa gcacggatcc gggttctacg tgtcgtcctg tgtcgcccgg tgttgttccc
     1201 tgcccctgcg tgtcctcctg tcgtgagcag cccgtgagca aggccggcgt cgcactcagg
     1261 ggtcagtaca acgacaatgc gtcccacgtg atctcctcgg catgttcggg cgagagcgtt
     1321 cgcaggacgc tttcgtaggt cagcccgcgt cgacttgcag cgcggacctc gcggccgcgg
     1381 atcccgatct gcctggcgat ctccgccatc gcgatcgaag cggtgagcct gtagcgatag
     1441 gagaagtcgg aggcaggaac atctatccgc gccagacgga cttcccttcc caggcgcccc
     1501 cagtcctcgg atccctcgtc gatcgaacct tcgtcgggaa gcacaagcac cgatgaaggg
     1561 ccactgtcca gccggaggcg gtgcaggggt ggcgcgtcga gcgaagtcga agctaccgtc
     1621 actcgatcga atccgggcgt gagcccttca taacgcgcga cccgtagggc ggctcgatgg
     1681 ctccgtgccg cagctcgtcc ctccgttcgg atcggccggg gggtgtcgaa ccagacgtgg
     1741 catcgatcct cgagagccac catcctccat ccgaacgctg cggccgtgcg aagggcgctg
     1801 cccgcctcga cgtgatctcg gggggccatc agcaagaggt tggggcgccg atcgggtcgt
     1861 ggcgtttgcg tgcggatcgt gcccgagcgg gaaagcgcgt gcaaggccac cgctgccgcc
     1921 gacgcaacgt tcaaggtatt gatgcgcgcc gacccggctg tggggatgtg gagtgtccgg
     1981 gtcgcaacgc gaaggacgtc tcgaccgatg ccacgacgtt cgtttccgac caccatcgaa
     2041 tggctgcctt taaccgtcac cgagaacaag gactcagagc cgcgcgtgtt ctcgatcgcc
     2101 acgatcggag tctgttcctg gagcagagcc ttcgaatcga tcgtcacaag ccgagagcct
     2161 ccgaggtgct cgtcccagcg ttccaggagg ccggacgtat ctcggaagag ggttgggatg
     2221 gcgaacatcg aggctgcatc catcagagcg cgagcgttgc gggggttctc cacgccgtcc
     2281 gctacgaggc tcccttgatg gctaccgccc cgcttcatcg gtccaagcct ttcggcaggc
     2341 aagcgcaaag gcgtcagccc tccgctcgtg atcagccgtt cgtttcttgc ggatgttccg
     2401 cttcccctcg tactgctgga tcacgtgatg gccgatctcg tgcaacagcc cgtcgaacgc
     2461 catgaagagc gagagcgagt catccggcca atcgatgacg gtagtcatct cggcgagatc
     2521 gatgagcgcc cccgcccggt gcagtcgaac cgccgttgcc tcgttcaatc tgccgggaag
     2581 cacccaggga ggcgtgggtt gctcgaacag aagaatcgaa tcaggtgaac ggtatctggc
     2641 aagagagatc gttccggctt gctcgcccgg gcggtggcgc agctcgaccg tcttgatgcc
     2701 gtacgtcccg atcggcccga cgaaggtgag catgcgtcgg atctctcggc ggtcgagcgg
     2761 atgtgcgaat ccgacgcgcg ggcgggtggt ggtgatccgg ggcagtgagc gatcctcatc
     2821 cgtcgcgatt ccctgaggga cgagggtgat gccaagttcc ttgaagaggc gcgcacgcgc
     2881 atgcgcgtcg ctgatgtcgc cagaacccct ggaggctctg ggatccttcg ctcgccgctc
     2941 cgcacgaatg cggggtgggt ccgtgtgctt cgtacgtgcc actcggtgag tatccctggc
     3001 ggggctggtt accgctcaca gcgacgtgcg ccgcctcgtg gccggcttcg tccggtgcag
     3061 ttctggcgtg agcgagcggt gagcaagatt gcttgttatc gcggcagctc gcaacgccga
     3121 ggacacgtat cggctgggga gattcgaact ccccacgccc tttcgggccc cggtcctaag
     3181 cccggcgtcg ctcagacccg gcctcgatcg cggcgtcgag cagctcgacg ggatgcacga
     3241 cgcgggcgtc gaccccgatc gccgcgaggc ccgcacggag ctgcatcgtg cagccggggt
     3301 tcgcgctcgc gatcacgcgc gcccccgtcg accggacagc ctccgtcttg tcgcgttgga
     3361 gtcgttcggc gagctcgggt tccgtgacgt tgtagaggcc ggcggcgccg cagcatcggt
     3421 cgccgtgggg gatctcgacg acctgcaggc ccgggatcgc gcccagcagc gcccgcggct
     3481 gtgcgtggat cccctgcgcg cgaagcgcgt gacacgcatc gtggtaggcg acgcgctcga
     3541 cgtcggcagg cgcgagctcg acggcgccga ggccctcgtc gtgcaggaac gcgatcacgt
     3601 cacgcacggg aagctctgtg ccgtccgcga gctcgccgta cgtctgcatg tgcgcaccgc
     3661 agccggccgc gttcgcgatc acgtgatcg
//
LOCUS       DATDBZ010000038         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2469114, whole genome shotgun sequence.
ACCESSION   DATDBZ010000038 DATDBZ010000000
VERSION     DATDBZ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2469114"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VIX62_01010"
     CDS             complement(<1..184)
                     /locus_tag="VIX62_01010"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY5858849.1"
                     /translation="MQPVALARVVRSDLEESVHLGHVAVCDAEGRLVAHAGDASHLVF
                     ARSCMKPLQAAVSLHAI"
     gene            complement(227..1018)
                     /locus_tag="VIX62_01015"
     CDS             complement(227..1018)
                     /locus_tag="VIX62_01015"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEY5858850.1"
                     /translation="MTITGVPHPAGMEGSHQDAELPTIHAQLTVFARELGGLYHAERL
                     RSRELDAALEDVREMYVSTMTSLAQIVEAKDSATRGHLDRTQRYGLALAARVDPDLAS
                     RPEVAYGFFLHDIGKVGIPESVLCKPGPLTDDEWTIMRSHPVIGAQIVEPIRFLQGAV
                     EIVRTHHERWDGAGYPAGLAGEQIPLAARVFAVADSFDAMTSDRPYRAALSIDEALDE
                     IRRGAATQFDPTVVGVFFDLVDDGELELEHERAHRIEIGQVAEAV"
     gene            complement(1180..1722)
                     /locus_tag="VIX62_01020"
     CDS             complement(1180..1722)
                     /locus_tag="VIX62_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HEY5858851.1"
                     /translation="MSDELNHPANRRIDRVRDPAYMEGLADLPLDDLRERRDECLAER
                     EYLSLLRRLVQGRAEILQAELTSRGDEIDRGPLVDRLNEILGSEERQGPGRGEAVRIG
                     VPEDEMMLARRRVERLVADAGISDPGSLDDEKLASAVGLLTAEEHSVSEARGAVIAAL
                     DTVQAELKRRYKQDPSLALR"
     gene            complement(1748..>2034)
                     /locus_tag="VIX62_01025"
     CDS             complement(1748..>2034)
                     /locus_tag="VIX62_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY5858852.1"
                     /translation="LGTPVVATHSGTVHRTGSSIGGYGIVLFHDGSADWTFYTHFSRY
                     GSYGEGAHVGAGQVIGYVGNTGTTVYHLHFEYHPGGGAAINPYSALLGVC"
BASE COUNT          331 a          676 c          699 g          328 t
ORIGIN      
        1 cgatcgcatg cagcgacacc gctgcctgca acggcttcat gcacgaacgc gcgaagacga
       61 ggtgggacgc gtcgccggcg tgggccacca accgcccctc cgcgtcgcac accgcgacat
      121 ggcccaggtg gacggattcc tccaggtccg aacggaccac acgcgccagc gcgacaggtt
      181 gcacgcccgc gaccatagcg ggcctgcggt ggacagaggc gcacggtcag acggcttcgg
      241 cgacctgacc gatctcgatc cggtgcgctc gctcgtgctc cagttccagt tcgccgtcat
      301 cgaccagatc gaagaacacg cccacgaccg tggggtcgaa ctgcgtcgcc gcgccacggc
      361 ggatctcgtc gagtgcttca tcgatcgaca gggccgctcg gtagggacgg tcgctcgtca
      421 tcgcgtcgaa cgagtcggcg accgcgaaca ctcgggccgc cagggggatc tgctccccag
      481 cgagtccggc cggataaccg gcaccgtccc agcgttcgtg atgggtgcgg acgatctcga
      541 cggctccctg aaggaagcgg atgggttcca cgatctgcgc cccgatcacc gggtgggatc
      601 gcatgatcgt ccactcgtcg tcggtgagcg ggccgggctt gcacaacacc gactcgggga
      661 tgcccacctt cccgatgtca tgcaggaaga acccgtaggc cacctcgggt cggctcgcca
      721 gatcggggtc gaccctggcg gcaagcgcga gtccgtatcg ctgggtgcga tcgaggtgtc
      781 cccgcgtggc gctgtccttg gcttccacta tctgggcgag cgaggtcatg gtcgagacat
      841 acatctcgcg aacgtcctcc agcgcggcat cgagctcgcg actgcgcagc cgttccgcgt
      901 ggtacaggcc acccagctcg cgcgcgaaga cggtgagctg ggcgtggatg gtgggcagtt
      961 cggcgtcttg atgcgacccc tccatgcccg ccggatgggg aacaccagtg atggtcacgg
     1021 tcacctcggt cgggaaagtc gcgtcacttt cgccgctcga gcccaccggt accatcggcc
     1081 gggcgagcga ttcgtcggag gccacacagc ccaggcgtga tggcccggct ggcctcggtc
     1141 atgtcggcga aggctcaccc cagacggggg acagtgggac tagcgcagcg ccagcgaggg
     1201 gtcctgcttg tagcgccgct tcagttcggc ttggacggtg tcgagcgcgg cgatcacggc
     1261 gccgcgagct tccgacacgg aatgctcttc ggcggtgagg agtccgacgg cggacgcgag
     1321 cttctcgtcg tccagagagc cggggtcgga gatgccggcg tcagcgacca gtcgctccac
     1381 gcgcctgcga gccagcatca tctcgtcctc gggcacgccg atgcggacgg cttcgccgcg
     1441 gcccggacct tgacgctcct ccgatcccag gatctcgttg aggcgatcga ccagcggtcc
     1501 acggtcgatc tcatcgcctc gagatgtcag ttcggcttgc aggatctcgg cgcgtccctg
     1561 caccagccga cgcaacaacg acaggtattc acgctcggcg aggcactcgt cgcggcgctc
     1621 tcggaggtca tcgaggggta ggtccgcgag accttccatg tacgcggggt cgcgaacacg
     1681 gtcgatccgg cggttggcgg gatggttcag ctcgtcgctc atcgcgcgcc atcctagccg
     1741 gagtcggcta gcagacgccc aggagcgcgg aatacgggtt gatggcggcg ccgccaccgg
     1801 ggtgatactc gaagtgcagg tgatagacgg ttgtgccggt gttgccgacg tagccgatga
     1861 cctgaccggc gccgacgtgg gcaccttccc cgtagctccc gtaccgagag aagtgcgtgt
     1921 agaaggtcca gtcggcactg ccgtcgtgga agaggacgat gccgtagccg ccgatggagc
     1981 tgccggttcg atgcaccgtg ccactgtgcg tggccaccac cggcgtgccc aagg
//
LOCUS       DATDBZ010000039         2657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_419971, whole genome shotgun sequence.
ACCESSION   DATDBZ010000039 DATDBZ010000000
VERSION     DATDBZ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419971"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VIX62_01030"
     CDS             complement(<1..202)
                     /locus_tag="VIX62_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5858853.1"
                     /translation="MEHVTSNDGTAIGYDRLGEGPPVVLVCGGSVDRMSNAPVAELLA
                     ADHTVFNYDRRGRGDSGDTQPYA"
     gene            complement(202..726)
                     /locus_tag="VIX62_01035"
     CDS             complement(202..726)
                     /locus_tag="VIX62_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5858854.1"
                     /translation="MADQTPEGVVSAHSDGNGIEIVRVFDAPRELVFKAWTEPERFAT
                     WFGEHGSSIPLDRIEMDARPGGAWSAVMLHGPDKIEIPFAGEFREVVEPERVVMTLAD
                     PGGTTSNVEVLTAEFKDIGDGRTEMTFTQRGGNLPADEYSRAMRGSLIFFDRLAEHVG
                     GETLKARHDQDGDS"
     gene            complement(719..1057)
                     /locus_tag="VIX62_01040"
     CDS             complement(719..1057)
                     /locus_tag="VIX62_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5858855.1"
                     /translation="MAVDQLSTTFGALADPTRRAILSRLSEGEATVSQLAEPFPISLQ
                     AVSKHLTVLEGATLITRGRVAQWRPCRLDAAPLGDVSAWLDHYRRFWEPRFDRLEDHL
                     RSIQEGATDG"
     gene            complement(1122..>2657)
                     /locus_tag="VIX62_01045"
     CDS             complement(1122..>2657)
                     /locus_tag="VIX62_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5858856.1"
                     /translation="EHHPSHFWLVFGVALLNVALGLIVSEVARRSGDERLFLVSMVLL
                     TSAGFLALHALATPGVVLDGPNGGFVLATPAGLILAAGFAALSAIEMDERTETRLRGL
                     QAPMRVGLVVVLIVWATASVAGFGPLDRVIESERAPWLLVLLPFAVAAYLFAAWRYLR
                     IYRTRRRPLPLAVAVAFVFLAEALIAMAFGRAWQASWWEWHVLMAIAFATIVLAARHE
                     YRHARTASGTFGGIYLERTLERLDHRQSAALAELVRADASGTLGSTGEELRQQGFTGD
                     QVAVMTDSARELARMDRLLRQYVSPHLADRLDAEPELAQLGGQEQEVTVLFADLSGFT
                     TFSEDRPAAEVIEMLNAYWGVVVPFVVDEEGGLIERFAGDAVLAVFNALGDQPDHAAR
                     AVRAAIAIRDRSAQVRASEDWPRFRVGVNTGVAVIGNVGAGSQRSFSVIGDTTNVAAR
                     LQTLSTPGHITIAGRTLDEVNTEPDVIVDVRPMGLADLKGKREPTEAFELLSVSATHD
                     DQR"
BASE COUNT          429 a          930 c          903 g          395 t
ORIGIN      
        1 cggcgtaggg ctgggtgtca ccgctgtcgc cacgaccacg gcggtcgtag ttgaagaccg
       61 tgtggtcggc cgccagcagc tcggcaaccg gtgcgttcga catgcggtcc accgacccgc
      121 cgcacaccag gaccacggga gggccttcgc cgagccggtc atagccgatc gccgtgccgt
      181 cgttcgatgt cacgtgttcc atcagctgtc cccgtcctgg tcgtgacgcg ccttcagggt
      241 ctcgccgccg acgtgctcgg cgagccggtc gaagaagatc agcgaaccgc gcatcgcgcg
      301 ggagtactcg tccgccggca ggttgccgcc gcgctgggtg aacgtcatct cggtgcggcc
      361 atcgccgatg tccttgaact cggcggtcag gacctcgacg ttgctggtgg taccaccggg
      421 gtccgccagc gtcatgacca cgcgctcggg ttccacgacc tcccggaact cccccgcgaa
      481 ggggatctcg atcttgtcgg gcccgtgcag catcacggcg ctccacgcgc cgccgggccg
      541 tgcgtccatc tcgatgcggt cgagcgggat cgaggaaccg tgctcaccga accaggtggc
      601 gaagcgttcg ggctcggtcc acgccttgaa cacgagttcg cgtggggcgt cgaacacccg
      661 cacgatctcg atgccgttgc cgtcgctgtg ggcggagacg acgccttcgg gtgtctgatc
      721 agccatcggt ggctccttcc tggatcgatc ggagatggtc ctcgaggcgg tcgaaccggg
      781 gctcccagaa ccgacggtag tggtccagcc atgcggagac gtccccgagc ggggcggcat
      841 ccaaacggca cgggcgccac tgtgcgaccc gcccccgggt gatcagcgtg gcgccctcca
      901 acacggtgag gtgtttggac accgcctgca ggctgatcgg gaacggttcg gccagctggc
      961 tgacggtggc ctctccttcg gacaggcggg acaagatcgc tcgccgcgtg ggatcggcga
     1021 gcgccccgaa cgtggtgctg agttggtcga ccgccatcgc gagatgcctt cctccgttaa
     1081 tcaaccgtta ggttgaacat agagcaacgc aaggagttcc gtcaacgttg atcgtcgtgg
     1141 gtggcgctga ccgagagcaa ctcgaaggct tcggtcggct cacgtttgcc cttcaggtcg
     1201 gccagcccca tcggccgcac gtcgacgatc acgtcgggct cggtgttgac ctcgtccagg
     1261 gtccggccgg cgatcgtgat gtggcccggg gtcgagaggg tctgcagacg ggcggcgacg
     1321 ttggtcgtgt cgccgatcac cgagaacgac cgttgcgacc cggcgcccac gttgccgatc
     1381 acggccaccc ccgtgttcac gccgacacgg aaccgcggcc agtcctcgga cgcgcgcacc
     1441 tgggcggaac ggtcgcggat cgcgatcgcc gcccgcaccg ctctcgccgc atggtcgggc
     1501 tggtcgccga gcgcgttgaa caccgcgagc acggcgtcgc cggcgaaccg ttcgatcaat
     1561 ccgccctcct cgtcgacgac gaacggcacc acgacacccc agtacgcgtt caacatctcg
     1621 atcacctcgg ccgccggccg atcctcggag aacgtggtga acccggagag atccgcgaac
     1681 aacacggtga cctcctgctc ctgcccgccc agctgcgcga gctcgggttc ggcgtcgaga
     1741 cggtcggcaa ggtgcgggct gacgtactgg cgcagcaggc ggtccatccg cgcgagttcg
     1801 cgcgccgagt ccgtcatgac ggccacctgg tcgccggtga acccttgctg acggagttcc
     1861 tcaccggtgc tgccgagggt ccccgaggcg tcggcacgca cgagctcggc gagcgcagcc
     1921 gattgtcggt gatcgaggcg ctccagggtg cgttccaggt agatgccgcc gaaggtgccc
     1981 gacgcggtgc gcgcatggcg gtactcgtgc cgggcagcga gcacgatcgt ggcgaatgcg
     2041 atcgccatca gcacgtgcca ctcccaccac gatgcttgcc acgcgcgccc gaacgccatg
     2101 gcgatcaagg cctcggcgag gaacacgaac gccaccgcga cggccagcgg aagggggcgg
     2161 cggcgtgtgc ggtagatccg caggtaccgc cacgccgcga acaggtaggc cgccacggcg
     2221 aacggcagca ggaccagcag ccacggcgcg cgttcggact cgatcacgcg atcgagcggt
     2281 ccgaacccgg ccaccgaggc cgtcgcccac acgatcaaca cgacgaccag gcccacccgc
     2341 atcggtgcct gcagcccacg cagcctcgtc tcggtgcgct cgtccatctc gatcgcggag
     2401 agcgccgcga accccgccgc caggatgagg ccggcggggg tggccagcac gaatccgccg
     2461 ttcggcccgt cgagcaccac gcctggtgtg gcgagcgcgt gcaacgccag gaaccccgcg
     2521 ctcgtgagca gcaccatcga caccaggaac agccgttcgt cgccgctccg ccgcgcgacc
     2581 tcgctcacga tcaggccgag ggccacgttg agcagggcga cgccgaacac gagccagaag
     2641 tgcgatggat ggtgctc
//
LOCUS       DATDBZ010000040         4132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2879775, whole genome shotgun sequence.
ACCESSION   DATDBZ010000040 DATDBZ010000000
VERSION     DATDBZ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2879775"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VIX62_01050"
     CDS             <1..1013
                     /locus_tag="VIX62_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY5858857.1"
                     /translation="TFGDPAAEYRAVREDVGMWDLSPLNKWDVRGTDAVEAVQRVNTN
                     DIAGMRVGQVRYGAFVDEDGLLVDDGTVYKHADDHLWMCTNGDERADYFADATKGLEV
                     SIEYIAPELPSMQIQGPRSRELMRTITDAPIDELAYFTFFPEQIQIGGIPAWVSRTGF
                     SGELGYEVFLRPEHAEALWEAVESAGARPYGVEIIEAIRVETGMIVTGYDYDEHERSP
                     YDLGLDRMVALDGAGEFMGKDELRTIATDPPNRFKTVKLEHDTLPEYGAALTKDGEDV
                     GVLTSPALSPILGPLGLAIIRTDVAVDGTQVDVAMPDGSTQAGTIDVLALYDPKKARP
                     RA"
     gene            1049..2143
                     /locus_tag="VIX62_01055"
     CDS             1049..2143
                     /locus_tag="VIX62_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY5858858.1"
                     /translation="MTDEHRSPLHEWHEAHGADTLVEDGWPWPMHEGTDPMVEYEAVR
                     TGTGIWDLFSTSKYEVTGPDAGRLIQRRFTNSLAGMQDGQVRYGAFVNADGKMIDDGN
                     VYKHAEDKYWVMINTPDIQDWFTETADGLDAQVAHRTEDFGMIAVQGPTAQGTLQGLT
                     DRDLGELKYFRFWPEPTKVAGKDAWVLRTGFSGEKGYEVVTAPEDSQAVWDALIAAGG
                     VPFGLTAIDLARTEVGLIIIAVDYNPNEISPWDLSMDKFIKTDTENVGAEALASYGAT
                     PPKRFKTLKIEGDAMPDYGAAVTKDGQQVGVVTSPAVSPRLGTIGLAILDAGVANDGE
                     KVEVAIEGGTAPATVEVLSLLDPAKEKPRA"
     gene            2210..3133
                     /gene="ftcD"
                     /locus_tag="VIX62_01060"
     CDS             2210..3133
                     /gene="ftcD"
                     /locus_tag="VIX62_01060"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEY5858859.1"
                     /translation="MTDGELYGVVPNFSEGRRRDVIEAIVEALHVPGARVVYAEADPD
                     HNRLDTTVLGDADAVRRSALAGAAAAVERIDMTQHEGGHPRMGAADVIPFMPVRGVGM
                     DACVELARAFGRELAETLDVPVYLYDRGALIPERASLADVRRGQFEGLRDAVATGERL
                     PDFGPNTIGRAGATAVGARKPLVAFNVYLDSTDEAGAKAIASALRESNGGLTAVRAIG
                     FAVPERGGIVTVSMNLVDHEVTGLRVAYDEVTRLASDHGMAVTDSEIVGLVPASALGQ
                     DDIDHLKLAGFDPDRQVLERLVDAAEEGTQA"
     gene            3130..3771
                     /locus_tag="VIX62_01065"
     CDS             3130..3771
                     /locus_tag="VIX62_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEY5858860.1"
                     /translation="MSDLELGALSVEGFCDVLASDSPTPGGGAVGAIEGAAAAALIGM
                     VGRLTIGKAGFEDLEQRMKDMVASADAARAEFLVLADRDAHAFDSVMVGFKMPKETDE
                     EKAARSAAIQAGYLAAASVPQEVARKAVDLMELAEDATAMGNPQAASDGLSSAASLYS
                     AALCAIANVEINAASLKDEGIRTEMLEELITLKARADELLEEAQTAFKLRLAS"
     gene            3813..>4132
                     /locus_tag="VIX62_01070"
     CDS             3813..>4132
                     /locus_tag="VIX62_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5858861.1"
                     /translation="MRIDAIVFDCLDAAPLARFWAEALGWRVAAYDQAELDRLADKGI
                     HDPEDDPSVMVEPPEDEMPGLPVLFFTEVPEDKAGKNRVHIDVVAQETVDEDVIRLEA
                     MGATL"
BASE COUNT          710 a         1392 c         1455 g          575 t
ORIGIN      
        1 ccacgttcgg cgatcccgcg gccgagtacc gcgcggtgcg cgaggacgtc ggcatgtggg
       61 acctctcgcc cctcaacaag tgggatgtgc gcggcaccga cgccgtcgaa gccgtgcagc
      121 gcgtcaacac gaacgacatc gccggcatgc gggtcggcca ggtccgttac ggcgccttcg
      181 tggacgagga cggtcttttg gtcgatgacg gcaccgtcta caagcacgcc gacgatcacc
      241 tgtggatgtg caccaacggc gacgagcgcg cggactactt cgccgatgcc acgaaaggac
      301 tcgaggtctc gatcgagtac atcgcgcctg agctccccag catgcagatc caggggccgc
      361 gttcgcgcga gctgatgcgc accatcaccg acgcgccgat cgacgagctg gcctacttca
      421 cgttcttccc cgagcagatc cagatcggcg gcatcccggc ctgggtgtcg cgaaccggat
      481 tctccggcga gctgggctac gaggtgttcc tgcggcccga gcacgccgag gcgctctggg
      541 aagcggtcga gtcagcaggc gccaggcctt acggcgtgga gatcatcgaa gcgatccggg
      601 tcgagaccgg catgatcgtc acgggctacg actacgacga acacgagcgc tccccgtacg
      661 acctgggatt ggaccggatg gtggcactcg acggggcggg cgagttcatg ggcaaggacg
      721 agctccgcac gatcgccacc gacccaccga accgcttcaa gacggtcaag ctcgagcacg
      781 acacgctccc cgagtacggc gccgccctga cgaaggacgg cgaggacgtc ggcgtgctca
      841 ccagccccgc gctcagcccc atcctgggac cgctgggcct cgcgatcatc cgcaccgacg
      901 tcgcggtcga cggcacccag gtcgacgtcg cgatgcccga cggctccacg caggcgggca
      961 cgatcgacgt gctcgcgctc tacgacccga agaaggcacg tcctcgcgcc tgacgcgagg
     1021 ctccgaaccc gtcgcacagg aggacacgat gaccgacgaa caccgctcac cgctgcacga
     1081 gtggcacgag gcgcacggcg ccgacacgct cgtggaggac ggctggccct ggccgatgca
     1141 cgagggcacc gacccgatgg tcgagtacga ggccgtccgc accggcaccg ggatctggga
     1201 cctgttctcc accagcaagt acgaggtgac cgggcccgac gcgggccggc tgatccagcg
     1261 ccggttcacg aactcgctcg ccggcatgca ggacggccag gtgcgctacg gcgcgttcgt
     1321 caacgccgac gggaagatga tcgacgacgg caacgtgtac aagcacgccg aagacaagta
     1381 ctgggtcatg atcaacaccc ccgacatcca ggactggttc accgagaccg ccgacggtct
     1441 cgacgcgcag gtggcccacc gcaccgagga cttcgggatg atcgcggtcc agggccccac
     1501 cgcgcagggc accctgcagg gcctcaccga ccgcgacctg ggcgagttga agtacttccg
     1561 cttctggccc gagcccacga aggtggcggg caaggacgca tgggtgctcc gcaccgggtt
     1621 cagcggtgag aaggggtacg aggtcgtcac ggcgcccgag gactcgcagg cggtctggga
     1681 cgcgttgatc gcggcaggcg gcgtgccgtt cggtctcacc gcgatcgatc tggcccgcac
     1741 cgaggtcggg ttgatcatca tcgccgtcga ctacaacccc aacgagatct cgccgtggga
     1801 cctgtcgatg gacaagttca tcaagaccga caccgagaac gtgggcgccg aggcgctcgc
     1861 cagctacggc gcgaccccgc cgaagcggtt caagaccctg aagatcgagg gcgacgcgat
     1921 gccggactac ggcgccgcgg tcacgaagga cggtcagcag gtcggggtcg tgacgtcccc
     1981 ggcggtcagc ccacgccttg gcacgatcgg gcttgcgatc ctcgacgcag gtgtcgccaa
     2041 cgacggcgag aaggtcgaag tcgcgatcga gggtggcacc gcgcccgcca cggtcgaagt
     2101 gctgtcgctg ctcgatcccg cgaaggagaa gccgcgcgcc tgacgcacgg tggtacgaac
     2161 cccgaagggg cgggccagac aggcccgccc ctttgcctat gctggggtga tgaccgacgg
     2221 tgagctctac ggcgtggtgc cgaacttcag cgaaggccgg cgcagggacg tgatcgaggc
     2281 gatcgtcgaa gccctgcatg tgcccggcgc tcgcgtcgtc tacgcagagg ccgaccccga
     2341 ccacaaccgg ctcgacacca cggtgctcgg cgatgccgac gccgtccggc gcagtgcgct
     2401 ggcgggagcg gccgcggccg tcgagcggat cgacatgacc cagcacgaag gcggacaccc
     2461 gcgcatgggc gccgccgacg tgatcccgtt catgccggtg cgcggcgtgg gcatggatgc
     2521 gtgcgtcgag ctcgctcggg ccttcggacg cgagctcgcc gagacgctcg acgttccggt
     2581 ctacctctac gaccggggtg cgctcatacc cgagcgggct tcgctggccg acgtccgacg
     2641 cggccagttc gaagggctgc gggacgcggt cgcgacgggc gaacggctcc cggacttcgg
     2701 tccgaacacg atcgggcggg ccggcgccac cgccgtgggc gcccgaaagc cgttggtcgc
     2761 gttcaacgtc tacctggact ccaccgacga ggccggcgcc aaggccatcg cctcggcgtt
     2821 gcgggagtcc aacgggggac tcaccgcggt gcgagcgatc gggttcgcgg tgcccgaacg
     2881 cggtggcatc gtcacggtgt cgatgaacct ggtcgaccac gaggtgacgg gcctgcgcgt
     2941 cgcgtacgac gaggtgacgc gtctcgcctc cgaccacggc atggccgtca cggattcgga
     3001 gatcgtgggt ctggtgccgg catcggcgct cggccaggac gacatcgacc acctgaagct
     3061 ggccgggttc gacccggacc gccaggtgct ggagcggctc gtggacgccg cggaggaggg
     3121 aacgcaagcg tgagcgatct cgaactgggt gcgctgagcg tcgaagggtt ctgcgatgtg
     3181 ctggcgtccg attcgcccac ccccggcggg ggagcggtcg gcgcgatcga aggggccgcc
     3241 gccgcggcgt tgatcggcat ggtgggccgg ctcacgatcg gcaaggcggg gttcgaggac
     3301 ctcgagcagc gcatgaagga catggtggct tcagccgatg ccgcccgtgc cgagttcctc
     3361 gtgctggccg accgcgacgc ccacgccttc gattcggtga tggtggggtt caagatgccg
     3421 aaggagaccg acgaggagaa ggccgcgagg tcggccgcga tccaggccgg gtacctcgcc
     3481 gccgcgtcgg tgccccagga ggtcgcgcgg aaagccgtcg acctgatgga actggcggaa
     3541 gacgccacgg cgatggggaa cccgcaagcg gcctccgacg ggctgtcgtc agccgcttcg
     3601 ctgtactcgg ccgccctctg tgccatcgcg aacgtcgaga tcaacgcggc gtcgttgaag
     3661 gacgagggca tccgaaccga gatgctcgag gaactcatca ccctcaaggc ccgtgccgac
     3721 gaactcctgg aggaagccca gaccgcgttc aagctgcgcc tcgcgtccta gctggtcttg
     3781 cgggccgccc cggcgtgggg caaggtcagg gcatgaggat cgacgcgatc gtgttcgatt
     3841 gcctcgacgc cgcgccgctg gctcgcttct gggcggaggc gctcggctgg cgcgtcgccg
     3901 cctacgacca ggccgagctc gaccggctcg ccgacaaggg catccacgat cccgaggacg
     3961 atccctccgt catggtggaa cctccggagg acgagatgcc aggtctgccc gtgctgttct
     4021 tcaccgaggt ccccgaggac aaggccggca agaaccgtgt gcacatcgat gtggtggcgc
     4081 aggagaccgt cgacgaggac gtgatccgtc tcgaagccat gggcgccacc ct
//
LOCUS       DATDBZ010000041         2196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1446594, whole genome shotgun sequence.
ACCESSION   DATDBZ010000041 DATDBZ010000000
VERSION     DATDBZ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446594"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VIX62_01075"
     CDS             complement(<1..216)
                     /locus_tag="VIX62_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017559650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY5858862.1"
                     /translation="MTRRRLDTELVRRGLASSRAEAQEAVRAGVVLVAGSPATKAATM
                     VADDQPVTLSAPARRFVSRGGDKLDAAL"
     gene            complement(242..673)
                     /locus_tag="VIX62_01080"
     CDS             complement(242..673)
                     /locus_tag="VIX62_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858863.1"
                     /translation="MVLDDLRKTLEATLGLLTPAKAQQLAKDMMDPGTAKEQIAKTAA
                     DLMDWSQSNRERLTTFIRAEISEQVKNVGVATSAELDSVKKRVRDLERDAGKTASGRK
                     TAARKTAARKTPARRKASTAKRSTTSGSGDHSGSTPDPSGA"
     gene            complement(766..1668)
                     /locus_tag="VIX62_01085"
     CDS             complement(766..1668)
                     /locus_tag="VIX62_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEY5858864.1"
                     /translation="MDLRRSCRTRVRGTGRSEASTLYSSRDRSNDRHPEGSARSVLAD
                     RYDAFLFDLDGVLYRGADPVPAAAEALTRLRAAGKRVAFVTNNASATPSSVAQRLGGA
                     GVEANVEEIETSALATAALLAERGVTSAFVVGEVGIKEALTQVGIRVVGAEQGEVEVV
                     VVGLDRTADYDSLRAAAMLIDRGAAFIATNPDGSFPAPGGVRWPGAGALVAAVEATTD
                     AKAEVVGKPNAPIFRAALARAGGGEPLVVGDRIDTDIAGAAALGWDSMLVLTGISTRD
                     EAARSATPPTYIAADLRALFEPVA"
     gene            1611..>2196
                     /locus_tag="VIX62_01090"
     CDS             1611..>2196
                     /locus_tag="VIX62_01090"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY5858865.1"
                     /translation="MPRISPFHGLVFDTTVAGPLDQVTAPPYDVISDRGRLDYLRMSP
                     YSVVHLDLAEGDDDPSLPDSRYTRAAHLLQDWRLRGIVTRSTEPRYYAYEMTFAIDGR
                     EHAIRGLLCAMDLEPWNGAIVPHERVMEGPVQDRLQLLRATRTHVSPVYGIIGGPCEP
                     LADLLGRLSGTPAPSRAQDEHGVTHSMWPIAADQA"
BASE COUNT          336 a          758 c          723 g          379 t
ORIGIN      
        1 caatgcggcg tcgagcttgt caccgcctcg ggagacgaac cgccgcgccg gtgcggacaa
       61 cgtgaccggc tgatcatcag ccaccatcgt ggctgccttg gttgcagggg aaccagcgac
      121 gaggacgaca ccggctcgga cggcttcctg cgcctcggcg cgactcgaag ccagaccgcg
      181 acgcaccaac tcggtgtcga gcctccgtct ggtcacggtg gtgtgcaccc tccgatgccg
      241 gttaggcgcc ggatggatcg ggggtcgaac cggagtggtc tcccgagccg gaggtcgtgg
      301 aacgcttggc cgtcgaagcc ttgcgacgcg cgggcgtctt gcgagcggcg gtcttgcgag
      361 cggcggtctt gcggcccgaa gcggtcttgc ccgcgtctcg ctctagatcg cgaacccgct
      421 tcttcacgga gtcgagctct gcgctggttg ccaccccgac gttcttcacc tgttcggaga
      481 tctcagcgcg gatgaacgtc gtgagccgct cacggttgga ctgtgaccag tccatgaggt
      541 cggccgccgt cttcgcgatc tgctccttgg cggtgccggg atccatcatg tccttcgcga
      601 gttgctgtgc tttggcggga gtgagcagtc cgagggtcgc ctcgagggtc tttcggaggt
      661 cgtcgagcac catgggaacg gtcctttcgc gggtgtcgcg ttgggttgac cccagcatac
      721 cgtcctgccg cggcgccctc gtgccgacgt cggctccgtc ccgatctagg cgaccggctc
      781 gaacagggct cgcaggtcgg cggcgatgta ggtgggcggc gtcgcgctcc gtgcagcctc
      841 gtcgcgcgtg gagatgcccg tgaggaccaa catcgagtcc caacccaacg cggctgcgcc
      901 ggcgatatcg gtatcgatcc tgtcgcccac caccaacggt tccccaccgc cggcacgcgc
      961 cagcgccgcc ctgaatatcg gcgcgttggg tttgccaacc acctccgctt tggcgtcggt
     1021 ggtggcttcg accgcggcga ccagtgcacc ggcgccgggc caccgaacac cgcccggtgc
     1081 ggggaaggat ccatccgggt tggtcgcgat gaacgcagcg ccccgatcga tcaacatcgc
     1141 ggcggcgcgc agcgagtcgt agtccgcggt gcgatcgagg cctaccacga ccacctcgac
     1201 ctcgccctgc tccgcgccca cgacccggat ccccacctgg gtcaaggctt ccttgatccc
     1261 gacctcgcca accacgaagg cactggtgac ccctcgttct gcgaggagtg cagccgtcgc
     1321 cagcgcggat gtctcgatct cttccacgtt ggcctccacc cccgcgcccc cgaggcgttg
     1381 ggccaccgac gacggtgtcg cgctcgcgtt gttcgtgacg aacgcgaccc gtttgccagc
     1441 tgcccgaaga cgggtcaacg cctcagcggc ggccgggacg ggatcggcac ctcggtacaa
     1501 gacgccgtcc agatcgaaga ggaaggcgtc gtagcggtct gcgaggaccg atcgagcgga
     1561 accttctggg tgtcggtcgt tgcttcggtc acgactgcta tacaaggtgg atgcctcgga
     1621 tctccccgtt ccacggactc gtgttcgaca cgaccgtcgc aggtccactc gaccaggtga
     1681 cggcccctcc ctacgacgtt atcagcgacc gtggccggct cgactatctg cgcatgagcc
     1741 cctacagcgt ggtgcatctg gacctggccg aaggggacga cgacccttcg ctccctgaca
     1801 gtcgctatac gcgtgccgcc cacctcctgc aggactggcg cctgcgcggc atagtgactc
     1861 gctcaacgga gccccgctac tacgcatatg agatgacctt cgcgatcgac gggcgtgagc
     1921 acgcgatccg gggattgttg tgcgcgatgg acctggaacc ctggaacggc gcgatcgtgc
     1981 cgcacgagcg ggtcatggaa ggaccggtgc aggaccggct gcagttgctg cgcgcgaccc
     2041 gtacccacgt gtcaccggtg tacggcatca tcggcggtcc gtgcgaaccg ctcgccgacc
     2101 tgttgggacg cctgtccggc acccctgccc catcgagggc acaggacgag cacggagtca
     2161 cccacagcat gtggccgatc gctgccgacc aggcga
//
LOCUS       DATDBZ010000042         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2470581, whole genome shotgun sequence.
ACCESSION   DATDBZ010000042 DATDBZ010000000
VERSION     DATDBZ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2470581"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VIX62_01095"
     CDS             <1..200
                     /locus_tag="VIX62_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY5858866.1"
                     /translation="VRLDPGLRPGLAFMTMHFPDDIDVNILTIDATDPKSGTAEFKAS
                     AVRIDTMPLDQVVGVSGGGGE"
     gene            205..2082
                     /locus_tag="VIX62_01100"
     CDS             205..2082
                     /locus_tag="VIX62_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY5858867.1"
                     /translation="MDLHLTQAGATDEERAAVDSLLGPPESSWDGAVERTEFDHRVAR
                     GGKDARGQRDLLLPTLHALQAGVGWISPGGLNYISERLTVPPAEAYGVATFYAMFSVE
                     ERPKSVVHVCDDLACRQVGGSELLSRLQGSQAHAMASPCLGLCEHAPAALIQVAGTDA
                     SDASVAPVTEEVERLLAAGGAVDGAIQGVSAPQPPDPLRLLRRVGVVDPTSIDDYRAH
                     GGFEALRLALEHGPEWTLREITDAKLMGRGGAAFPTGVKWKAVAEQPVHPHYFICNAD
                     ESEPGTFKDRVVMEQDPFAVIEALTIAGITTGCELGYLYIRGEYPLATQRLEHAIVEA
                     RHHGFLGADVMGHGATFDIELRRGAGAYICGEETALFNSLEGKRGEPRNKPPFPVQRG
                     VFGKPTGINNVETLINVLEVLPIGGQAYAELGTEGSTGPRLFCLSGAVTAPGVYEFEH
                     GITLGEVIDAAGGVRDGKQLKAVLLGGAAGGFVGPDRLDVRMTFEDARAGGYTLGSGV
                     VMVFDEDADLTDACLRIARFFRDESCGQCVPCRVGTVRQEEALHRMVQGRMLGSHQDE
                     LQLLEDVAQVMRDASICGLGQTAANAIQSAIASLGVFGGDTGDTLQGDAVPTATEEQE
                     S"
     gene            2085..>2381
                     /locus_tag="VIX62_01105"
     CDS             2085..>2381
                     /locus_tag="VIX62_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5858868.1"
                     /translation="MASAVPLEPPRRLFTMEVDGTEVRVADGATILDACRALEVDTPT
                     LCFADNLTPVNACRVCVVEVEGARTLVPACSRKAEDGMKVKTDTERVRHSRTLVM"
BASE COUNT          382 a          787 c          834 g          378 t
ORIGIN      
        1 cggtgcggct ggatccgggg ctacggcccg ggctcgcgtt catgaccatg cacttcccgg
       61 acgacatcga cgtcaacatc ctcacgatcg acgccaccga cccgaagtcc ggcaccgcgg
      121 agttcaaggc ctcggccgtc cggatcgaca cgatgcccct cgaccaggtg gtcggcgtgt
      181 cgggtggggg cggggagtag ctggttggat ctccatctga cccaggccgg ggccaccgac
      241 gaggaacgtg ccgccgtcga ttcgctgcta ggcccgcccg agagttcatg ggacggcgcc
      301 gtcgagcgca ccgagttcga ccatcgggta gcgcgaggcg gcaaggacgc gcgcggccaa
      361 cgcgatctcc tgttgcccac gctgcacgcg ctccaggcag gcgtgggctg gatcagcccc
      421 ggaggcctca actacatcag cgagcgactc acggtgccgc ccgccgaggc gtacggggtc
      481 gccaccttct acgccatgtt cagcgtcgag gaacgaccga agtccgtcgt gcatgtgtgc
      541 gacgacctcg cgtgccgcca ggtcgggggt tcagagctcc tgtcccgact ccaggggtcg
      601 caggcccacg cgatggccag tccctgtctc ggcctgtgcg aacacgcgcc ggcggccctg
      661 atccaagtgg cagggacgga cgcgtctgac gcctcggtgg cgcccgtcac cgaggaagtg
      721 gagcggctcc tcgccgccgg cggcgcggtc gatggtgcga tccagggggt cagcgcaccg
      781 cagcctcctg acccactccg actcctgcgc cgcgtcgggg tcgtcgatcc cacctcgatc
      841 gatgactacc gggcccatgg cgggttcgag gcgctgcgac tcgcgctcga gcacggtccg
      901 gaatggacct tgcgagagat caccgacgcc aagctcatgg gtcgtggcgg tgcggcgttc
      961 cccaccgggg tcaagtggaa ggcggtcgcc gaacagccgg tgcacccgca ctacttcatc
     1021 tgcaacgccg acgaatccga gcccggcacg ttcaaggacc gggtcgtgat ggagcaggat
     1081 ccgttcgcgg tgatcgaggc acttacgatc gccggcatca cgaccgggtg cgaactgggc
     1141 tacctctaca tccgaggtga gtacccgttg gcgacccaac gtttggaaca tgcgatcgtc
     1201 gaggctcgcc atcacggatt cctcggtgcg gatgtcatgg gccacggtgc cacgttcgac
     1261 atcgagttgc ggcgtggcgc gggcgcctac atctgcggcg aggagacggc gttgttcaac
     1321 tcgctggaag gcaagcgcgg tgagccgcgc aacaagcccc cgttcccggt gcagcggggc
     1381 gtgttcggca agcccaccgg catcaacaac gtcgagaccc tgatcaacgt gctcgaggtg
     1441 ctgcccatcg gtggccaggc gtacgcagaa ctcggcaccg aaggttcgac gggaccgcgc
     1501 ttgttctgtc tgtccggcgc cgtgaccgcg cccggcgtct acgagttcga acacggcatc
     1561 acgctgggcg aagtcatcga cgcggcgggt ggcgtccgcg acggcaagca gctgaaggcg
     1621 gtgctgctgg gtggcgcggc gggtggattc gtgggacccg atcggctcga cgtccgtatg
     1681 acgttcgaag acgctcgcgc tggtggctac acgttgggct cgggcgtcgt gatggtgttc
     1741 gacgaggatg cggatctcac cgatgcgtgc cttcgcatcg cgcggttctt ccgcgatgaa
     1801 tcgtgcggcc aatgtgtgcc gtgtcgcgtg ggcaccgtgc gccaggaaga agccctccac
     1861 cggatggtgc aaggacgcat gctgggaagt caccaggacg agctccagct gctcgaggac
     1921 gtcgcccagg tcatgcgcga tgcgtcgatc tgcggcctgg gccagaccgc cgcgaacgcg
     1981 atccagtcgg cgatcgcatc gctcggcgtg ttcggtggcg acaccggcga tacgctccag
     2041 ggcgacgcgg tgcctaccgc gaccgaggaa caggagtcgt agcgatggcg tccgccgtgc
     2101 ccctggaacc gccccgccgc ctgttcacga tggaggtgga cggcaccgag gtgcgcgtcg
     2161 ccgatggcgc cacgatcctg gatgcgtgcc gtgccctcga ggtcgacaca ccgacgctct
     2221 gtttcgccga caacctcacg ccggtgaacg cctgtcgcgt gtgcgtcgtc gaggtcgaag
     2281 gcgcccggac gttggtgcct gcctgcagcc ggaaggccga agacggcatg aaggtcaaga
     2341 ccgacaccga gcgggtgcgg cactcccgca cgctggtcat g
//
LOCUS       DATDBZ010000043         2256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_630075, whole genome shotgun sequence.
ACCESSION   DATDBZ010000043 DATDBZ010000000
VERSION     DATDBZ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630075"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VIX62_01110"
     CDS             <1..979
                     /locus_tag="VIX62_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5858869.1"
                     /translation="FDAAAYERFAALADETFRQVSERTGIPLELLMVVREAIGMAPHS
                     PDDRLREDEAAIVPFIELQLAEGFRPAGIERLLRVQGDSIRRMTEQEGAWWHSEVIEP
                     ALAAGKSPDDVGHAELSDRISPLAEQSVLAMYHAQQTRAWTANIIGAFEVMMERAGIH
                     SRLERLPAICFLDISGYTRLTQERGDDAAADVAATVARLVRRNSLEHGGKPIKWLGDG
                     VMFHFPEPGPGVLAALEMVSGLATAGLPPCHVGLHAGPVLYQEGDYFGQTVNLSARIA
                     DYARANEVLVSAAVADASQDDGVSFDDIGPVELKGVSGTVHLLRAGLAM"
     gene            1020..>2256
                     /locus_tag="VIX62_01115"
     CDS             1020..>2256
                     /locus_tag="VIX62_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5858870.1"
                     /translation="MSDLAGIDGLDDAAVTIYANGTVFTGNRQRPWAEACAVRGSTVL
                     GVGPTSEVRRDHPRAIEHDLDGGTLLPGLIDAHNHFLSTGESLGSLDLRYPGVDSAGA
                     LLQAIRDAAEITSPGDPITGFGFDNAKYELPALAELDAAAGNHPLHLFHTSGHNVLVN
                     TVVFAEAGVDETASDPPGGRFVRDRDGRLTGLCLDAACGVVLPTAVDIGSHGPNFHTQ
                     APIDALVAAVDRASRAYVEAGLTCVADAQVTARELAAYREARARGVLGVRTVCMPLSH
                     QLEAFRSVGLVGPFGDGELSIGHLKIYADGSLTGGTAAFSQELHVRGQEASFFHEPGA
                     LVELIERAWSSGWRIAVHAQGDRAISCVLDGFEKGHASGSHDDPRPRIEHCGYPGIVG
                     IQRMRAVGAIAVNQPSYLHD"
BASE COUNT          360 a          768 c          769 g          359 t
ORIGIN      
        1 cttcgacgcc gccgcctacg aacggttcgc cgccctggcc gacgagacat tccggcaggt
       61 cagcgagcgg accggcatcc cgctcgagct gctgatggtc gtcagggagg cgatcggcat
      121 ggcaccccac tcgcccgacg atcggttgcg agaggacgag gccgcgatcg tgccgttcat
      181 cgaactgcag ctcgccgagg gcttccgtcc ggcgggcatc gagcggctgc tccgcgtcca
      241 gggcgacagc atccggcgga tgaccgagca ggaaggcgcc tggtggcact ccgaggtgat
      301 cgagcccgct ttggccgcgg gcaagagtcc ggatgacgtc gggcacgccg agctctccga
      361 ccggatctca ccgctcgcgg agcagagcgt cctggccatg taccacgcgc agcagacgcg
      421 cgcttggacg gcgaacatca tcggcgcgtt cgaagtgatg atggagcggg cggggatcca
      481 cagtcgcctc gagcggctcc cggcgatctg cttcctcgac atcagcgggt atacccggct
      541 gacgcaggaa cgcggcgacg atgcggcggc cgacgtcgcc gctaccgtcg cacgcctggt
      601 gcgacgcaac tctctggagc acggcggcaa acccatcaaa tggctcggcg acggcgtgat
      661 gttccacttc cccgaacccg gcccgggtgt gctcgcggcg ctcgagatgg tgagcgggct
      721 cgcgaccgcc ggactgccac cgtgccacgt gggactgcat gcgggcccgg tcctgtacca
      781 ggagggcgac tacttcggtc agacggtcaa cctcagcgcg cggatcgccg actacgcgcg
      841 ggcgaacgaa gtgctggtca gcgccgcggt cgccgacgcc tcgcaagacg acggcgtgtc
      901 gttcgacgat atcggtcccg tggaactcaa aggcgtgtcg ggaacggtgc acctcttgcg
      961 ggccggcctg gccatgtaac aggaactggc ccaaggccaa tgagacgctg cctgaaagga
     1021 tgagtgatct ggccggcatc gacggcctcg acgacgctgc cgtcacgatc tatgcgaacg
     1081 ggacggtgtt caccgggaac cggcagcgcc cgtgggccga ggcatgcgcg gtccgcggct
     1141 cgaccgtgct gggggtcggt cccacatcgg aggttcgccg cgaccatccc cgcgcgatcg
     1201 agcacgacct cgacggtggg acgctcctcc ccgggttgat cgatgcccac aaccatttcc
     1261 tgtccaccgg tgagtcgctc ggctccctcg acctgcgcta cccgggtgtg gattccgctg
     1321 gggcgctcct ccaggcgatc cgcgatgccg ccgagatcac ctcgccgggc gatccgatca
     1381 cgggattcgg gttcgacaac gcgaagtacg agctgccggc gctggccgag ctcgacgccg
     1441 ccgctgggaa ccatcccctc catctcttcc acacgtccgg gcacaacgtc ttggtcaaca
     1501 ccgtggtctt cgcggaggcc ggggtcgatg aaacggcctc cgatcccccg ggtggacggt
     1561 tcgtgcgtga tcgggacggg aggctgacgg gtctgtgtct cgatgccgcg tgcggcgtcg
     1621 tgttgccgac cgccgtggac atcggttcgc acggaccgaa cttccacacg caagcaccga
     1681 tcgacgcctt ggtcgcggcg gtggatcggg ccagccgcgc ctacgtcgag gcaggtctga
     1741 cctgcgttgc cgatgcccaa gtcaccgccc gcgagctcgc cgcctatcgc gaggcgcgcg
     1801 cccgtggggt cctcggcgtg cgcacggtct gcatgcctct ttcccaccag ctcgaggcgt
     1861 ttcgaagcgt cggactcgtc ggcccgttcg gcgacggtga actctcgatc ggccatctga
     1921 agatctacgc cgacggctcg ctcacgggtg ggacggctgc gttcagccag gagctccacg
     1981 tccgagggca agaagcctcg ttcttccacg aacccggagc actcgtcgag ttgatcgagc
     2041 gcgcgtggtc gtcgggctgg cggatcgcgg tccacgcgca aggggaccgt gcgatctcct
     2101 gcgtgctgga cggcttcgag aagggacacg cgagtggatc ccatgacgat ccgcgtcccc
     2161 ggatcgaaca ctgcggatat cccggcatag tcggcatcca acgcatgcgt gccgtaggag
     2221 ccatcgcggt gaaccagccg tcatacctgc acgact
//
LOCUS       DATDBZ010000044         4806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1033579, whole genome shotgun sequence.
ACCESSION   DATDBZ010000044 DATDBZ010000000
VERSION     DATDBZ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033579"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1300
                     /gene="aroF"
                     /locus_tag="VIX62_01120"
     CDS             287..1300
                     /gene="aroF"
                     /locus_tag="VIX62_01120"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY5858871.1"
                     /translation="MKQDATDSDIELVCEKVRNAGGEAFVSRGAVHTVVGLVGDTARF
                     QAIPFTQLHGVDHVIQIGKPYKMVARDLHPEATTVNVGSTPLGRGTFTIIAGPCAVES
                     EEQAMIGTRAAKAAGATILRGDIYKPRTSPYSFQGLGERGLEILIECRRDTGLPLVIE
                     VVDVSQVAHVAEVADCIRVGTRNAQNFELLKEVGLTKKPVMLKRGLAMTIDEWLQAAE
                     YVAQRGNSEIILCERGIRTFEPATRNTLDLAGMAVAQHESHLPVIVDPSHAVGKKHLV
                     APMARAAVAAGADGVMIDVHHDPEQALCDGPQALLPEEMAALGKELATLAEAMGRPVN
                     RPE"
     gene            complement(1318..1788)
                     /locus_tag="VIX62_01125"
     CDS             complement(1318..1788)
                     /locus_tag="VIX62_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5858872.1"
                     /translation="MRNADLLPQVSPWELMVTLRRCQHQLEVFMDESLDGMGMSFAQY
                     RALEAISEIGPMHIAYLARLLKVSRQAAQRSARQLANHGLVDLRPEGREVVIEITPEA
                     RRRLELCRTATSRLRESLADRLSEAQRSSLASLLRDAERAFQPPPPRVRPWWLD"
     gene            complement(1981..2760)
                     /locus_tag="VIX62_01130"
     CDS             complement(1981..2760)
                     /locus_tag="VIX62_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858873.1"
                     /translation="MELVGELVAAAVVDARVWEAYGNKANDGVYLVGQPGPALPFVIF
                     RAWKVPVGFVTEEVRLIGPSGRTIHRWGPEVRRMRGAMDLTVELDHVEDAVFDESGTY
                     VASFILDDQIVGEIDFPVFVQTSAEKLPKETEDGLKKSDVIWVGYERGGRRRTAPVWF
                     AYKNGKIYVVSQREVGPEEQTVPGVPDAQEMLVITRRKGRDTSLEEFTAAQRVLEGAE
                     WEEAAKWLVDKRKSRAGAPAESIQRWRGSADIIELTPNLPS"
     gene            complement(2789..3973)
                     /locus_tag="VIX62_01135"
     CDS             complement(2789..3973)
                     /locus_tag="VIX62_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY5858874.1"
                     /translation="MSDPPGGLGGFPFGMDPESLRDAPLFREMQRVMASSSGPINWEL
                     ARQVGVASAVEAGEDREPTDDERSGFTEAVRVAELHVARFTGLDAPTDVAEVRAIRRA
                     EWINANAESMKALLEPAAQRVSEAMMRAQGTSMPAEMQAMSGMLAQISPLLMGAQVGS
                     VLGFLAQRVLGQYDVAVPRSGPGAVLFVAANIRQFESDWSLDPTDFRTWVAFHEVTHR
                     FEFARPWARDRFSELLDDFLSTLTIDVEGMQAKLMTMDGADPEALQGLLEGDEGLFGA
                     ELDDEQRIKLGRIQSFMAAAEGYGDHVMHALGAELLPSYGRIEEAMRRYREGEAGDPV
                     FERLLGIEMKREQYVNGRAFCDTVVELTDEATLARMWDSAEAMPSLPEIGEPRLWLAR
                     TV"
     gene            4013..4465
                     /locus_tag="VIX62_01140"
     CDS             4013..4465
                     /locus_tag="VIX62_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY5858875.1"
                     /translation="MSRPSSPLRSSTRILARVQADPLSADEALAFVADPGAGGTCVFL
                     GTVRDHSNAGDVTGLTYEAWDDLAVKRLEEIAGEARDRWDLCAVSLLHRTGALQVGET
                     SVIVAASAPHRADAFEAARHAIERLKEDVPIWKKEGLVTGESHWIMGS"
     gene            4467..>4806
                     /locus_tag="VIX62_01145"
     CDS             4467..>4806
                     /locus_tag="VIX62_01145"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5858876.1"
                     /translation="MGRDLAIDLGTANTLVYRQGDGIVFDEPCVVAMHSGTGEVVAMG
                     EEAWQMIGGDSGSVVAVRPLRKGTMTEFDITQRMLEVVIRKVGATRFPRPRVLVAVPS
                     MASEVEKRAIE"
BASE COUNT          765 a         1649 c         1633 g          759 t
ORIGIN      
        1 tcagcaaatc gccggcgagc gggtgatcga ccacggtgaa ggagggcatc cggcgcatcc
       61 tacgtgggtt cgtggggcgt tcgcttgcca ctgcggggtc gcgtcgggca tcatgggcgg
      121 gacatggagc gcttcttcgc ctgctggttc acctacttcg ggcctgaccc ggggtagtcg
      181 ccggtagcct tcgagccaac agcgaccccg ggtccgacac ccggggtttt cccttccccg
      241 ggaccggccc ggccgatcgg gaggtcaaca gcacatggtc gtcgtcatga aacaggacgc
      301 gacggactcg gacatcgagc tcgtctgcga gaaggtgcgc aacgccgggg gcgaagcgtt
      361 cgtctcgcgt ggcgccgtcc acaccgtggt gggcctggtg ggcgacacgg cgcgtttcca
      421 agcgatcccg ttcacgcagc tccacggcgt cgaccacgtg atccagatcg gcaagcccta
      481 caagatggtg gcgcgcgacc tgcatccgga agccaccacg gtgaacgtgg gttcgacgcc
      541 gctgggccgc ggaacgttca cgatcatcgc ggggccgtgt gcggtggagt ccgaggagca
      601 ggcgatgatc ggcacccgcg cggccaaggc cgcgggcgcc acgatcctgc gtggggacat
      661 ctacaagccg cgcacctcgc cgtattcgtt ccaggggttg ggggagcgtg ggctcgagat
      721 cttgatcgag tgtcgccgag acaccgggtt gccgctcgtc atcgaggtgg tcgatgtgtc
      781 gcaggtggcg cacgtggccg aggtggccga ctgcatccgg gtgggcacgc ggaacgcgca
      841 gaacttcgag ctgctgaagg aggtcggcct cacgaagaag cccgtgatgc tgaagcgggg
      901 cctggcgatg acgatcgacg agtggctgca ggcggccgag tacgtggccc agcgcggcaa
      961 cagcgagatc atcctgtgcg agcgggggat ccggacgttc gagcccgcga cccggaacac
     1021 gctggacctg gcggggatgg cggtggccca gcacgagtcg catctgccgg tgatcgtcga
     1081 cccgtcccac gcggtcggca agaagcatct ggtggcgccg atggctcggg cagcggtcgc
     1141 cgcgggagcg gacggcgtga tgatcgatgt ccaccacgat ccggagcagg cgctgtgcga
     1201 cggcccgcag gcgctcctgc ccgaggagat ggcggcgctg ggcaaggagc ttgcgacgct
     1261 cgccgaagcg atggggcgtc cggtcaaccg acccgagtag ggcggttcgc ggcttcgtca
     1321 gtcgagccac cacgggcgga cgcgtggcgg agggggctgg aacgcccgct cagcgtcgcg
     1381 gagcagggat gcgagcgacg accgttgtgc ctcggagagg cgatctgcga gcgactcacg
     1441 gagtcggctg gtcgctgtcc ggcagagttc cagacgtcga cgagcctcag gggtgatctc
     1501 gatgacgacc tcgcggcctt caggccgcag gtcgacgagg ccatggttcg cgagctgacg
     1561 agcgcttcgc tgagccgctt gacggctcac cttgaggagt cgagcgagat aggcgatgtg
     1621 catcgggccg atctcgctga tggcttcgag ggcgcggtac tgagcgaacg acatccccat
     1681 gccgtcgagg gactcgtcca tgaacacctc gagctgatgc tggcaacgac ggagcgtgac
     1741 catgagttcc cacgggctca cctgcggcag gaggtcggcg tttcgcatcg ggcagtctta
     1801 cctcgccccg gtgacaccgg aatcgagagc gtccggtgtt ctgctcagcg gaccgtcaac
     1861 aggtatacaa gttgcttgct gtgatggaac ccgtacgccg ttgacgatcg gcggggcgag
     1921 gggacgtcat cattgttcaa gagaacaacc tttacccatc gccacgggtc ggggcggtga
     1981 ctagctgggc aagttcgggg tgagttcgat gatgtcggcg ctcccgcgcc accgttggat
     2041 cgactccgcc ggcgcccccg cgcggctctt ccgcttgtcg accaaccact tcgcggcctc
     2101 ttcccactcc gcgccctcga gcacccgctg cgccgccgtg aactcctcca gcgacgtgtc
     2161 tcgacctttg cgtcgcgtga tcaccagcat ctcctgcgcg tccgggacgc cgggcacggt
     2221 ctgctcctcc ggccccacct cgcgctgcga caccacgtag atcttgccgt tcttgtacgc
     2281 gaaccacacc ggcgccgtgc gccgtcgccc gccccgctcg tagccgaccc agatcacgtc
     2341 ggacttcttc agcccgtcct cggtctcttt cggcagcttc tccgccgacg tctgcacgaa
     2401 caccgggaag tcgatctcgc ccacgatctg gtcgtccaag atgaacgacg ccacgtacgt
     2461 gcccgactcg tcgaacaccg cgtcctcgac atgatccagc tccaccgtca ggtccatggc
     2521 cccgcgcatg cggcgaacct caggtcccca ccggtggatc gtgcgccccg acgggccgat
     2581 cagcctgacc tcctcggtca cgaagcccac cggcaccttc cacgcccgga agatcacgaa
     2641 cggcagcgcc ggtccgggct ggcccaccag gtacacaccg tcgttggcct tgttgccata
     2701 ggcctcccac acgcgggcgt cgaccacggc ggcggcgacg agttctccga ccagctccat
     2761 cgagcggaga ggatacccga ggaaggggtc agaccgtgcg cgcgagccac aggcgcggct
     2821 cgccgatctc gggcagcgat ggcatcgcct cggccgagtc ccacatccgc gccagggtgg
     2881 cctcgtccgt gagttccacc accgtgtcgc agaacgcgcg cccgttcacg tactgctcac
     2941 gcttcatctc gatgcccagc agccgctcga acaccggatc cccggcctcg ccctcgcggt
     3001 agcggcgcat cgcctcctcg atccggccgt acgacggcag cagctcggcg cccagcgcgt
     3061 gcatcacgtg gtcgccgtag ccctcggcag ccgccatgaa cgactggatc cgccccagct
     3121 tgatccgctg ctcgtcgtcg agttcggcgc cgaacaagcc ttcgtcgccc tccagcaatc
     3181 cctgcagggc ctcgggatcg gcgccgtcca tcgtcatcaa cttcgcctgc atgccctcga
     3241 cgtcgatcgt gagcgtcgac aggaaatcgt ccagcagctc cgagaaccgg tcgcgcgccc
     3301 acggccgggc gaactcgaac cggtgggtca cctcgtggaa cgccacccag gtacggaagt
     3361 cggtggggtc gagcgaccag tcgctctcga actgacggat gtttgccgcc acgaagagca
     3421 ccgcgcccgg cccggatcgg ggcacggcca cgtcgtactg gcccagcacg cgttgggcca
     3481 ggaagccgag gaccgagccc acctgcgcgc ccatcagcaa cggcgagatc tgcgccagca
     3541 tgccgctcat ggcctgcatc tcggcgggca tggaggtgcc ctgggcgcgc atcatcgcct
     3601 cggacacgcg ttgcgcggcc ggctccagca gcgccttcat gctctcggcg ttcgcgttga
     3661 tccactcggc cctgcggatc gcccggacct cggcgacgtc ggtgggcgca tccaggccgg
     3721 tgaaccgcgc cacgtgcagc tcggcgacgc gcaccgcttc ggtgaagccc gagcgttcgt
     3781 cgtcggtcgg ctcgcggtcc tcgccggctt cgacggcgct cgccacgccc acctggcgcg
     3841 cgagctccca attgatgggt ccgctcgagg acgccatcac ccgctgcatc tcacggaaca
     3901 acggcgcgtc gcgcagggac tcggggtcca tcccgaacgg gaaaccgccg aggccaccgg
     3961 gagggtcgct catcccgcca tcctacgacc gcgccttcgt tacgatggcg ccgtgtcacg
     4021 gccctcatca ccgctgcgtt cgtccacgcg catcctcgcg cgggtgcagg cggatccact
     4081 gtcggcggac gaggcgttgg cgttcgtcgc cgaccccggc gcgggcggca cctgcgtgtt
     4141 cctggggacg gtgcgcgacc attcgaacgc cggggatgtc accggactca cctacgaggc
     4201 ctgggacgac ctcgccgtga agcgcctgga ggagatcgct ggagaggcgc gcgaccgctg
     4261 ggacctgtgc gcagtgtcgt tgctccaccg gaccggcgcc ttgcaagttg gggagaccag
     4321 cgtgatcgtc gccgcttcag ccccccatcg ggccgacgcc ttcgaagcgg cccgccacgc
     4381 gatcgaacgc ctcaaggaag acgtccccat ctggaagaag gagggcctcg tcaccggcga
     4441 gtcccactgg atcatgggga gctgacgtgg ggcgggatct cgcgatcgac ctgggcacgg
     4501 cgaacacgct cgtctaccgc cagggcgacg gcatcgtgtt cgacgagcca tgtgtcgtgg
     4561 cgatgcactc cgggacgggc gaggtcgtcg cgatgggtga ggaagcctgg cagatgatcg
     4621 gcggcgactc cggcagcgtg gtcgcggtgc gtcccttgcg caaggggacg atgaccgagt
     4681 tcgacatcac ccaacgcatg cttgaagtcg tgatccgcaa ggtgggagcc accaggttcc
     4741 ctcggcctcg cgtgctcgta gcggtgccgt cgatggcctc agaggtcgag aagcgcgcga
     4801 tcgaag
//
LOCUS       DATDBZ010000045         2998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2266871, whole genome shotgun sequence.
ACCESSION   DATDBZ010000045 DATDBZ010000000
VERSION     DATDBZ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266871"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1135)
                     /locus_tag="VIX62_01150"
     CDS             complement(47..1135)
                     /locus_tag="VIX62_01150"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEY5858877.1"
                     /translation="MKVQVGVATDIGRVREGNEDSYLIEPPLYAVADGMGGHRGGEVA
                     SQLALETIEDLFRKDEGSLIEQVHSANHAVFMQSQEDTAVSGMGTTLTAALVEGSQLQ
                     LAHVGDSRAYLLRADSLRQLTADHTLVARMVKTGEITEAEAEVHPHRNVLTRSLGTEA
                     DVQVDEDVVGLLDGDRLLLCSDGLTGMVTEDQIKAILETEPDPQRASDRLIKAANRAG
                     GIDNITVVVLDAIGEDDDPVGPAPDAPRWRRWLIGGLVALVLLVGTFFAARTFIDRQW
                     YVGVADDKVAVYQGIPAKPLSLSLSSVDTITEIPAADAGALELYRDLPQGITADSRED
                     ALAIVERIRTDVADDAVPPKKPKNAGGQ"
     gene            complement(1132..1647)
                     /locus_tag="VIX62_01155"
     CDS             complement(1132..1647)
                     /locus_tag="VIX62_01155"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5858878.1"
                     /translation="MTASLAPPAIPFVAEGVTPFALSALKYGLFALLVLFLWRSMRWA
                     VRGLSVEAAPARGGRSARKARDAAEGPTVTPGISDVVVHADGAKPRTVRVASDSLVIG
                     RAPECELAIDDTYVSQQHARVFAKGGSWYVEDLGSTNGTFVNDQRLSSPAMVQAGDKV
                     RIGTTVMELRR"
     gene            complement(1650..2339)
                     /locus_tag="VIX62_01160"
     CDS             complement(1650..2339)
                     /locus_tag="VIX62_01160"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEY5858879.1"
                     /translation="MPNLRDFERRLGGLVEGLFSKTFRSGVQPVELAKRLIKEMDAER
                     TVGPNGVWAPNHFEFALSTEDAERFEQAETALASELAQVARENADERGWGLVGPPEIT
                     FTTDASLKRGAITCVATLVQGPDEPAPSSAPSASAMLLIRENGAERRVPIAKEVVTIG
                     RMSDCDVVLKDKGASRRHVQLRTSEGVTTLTDLGSTNGTRLNGQTVQSGALSDGDRIT
                     IGTTVLEYRRA"
     gene            complement(2390..2749)
                     /locus_tag="VIX62_01165"
     CDS             complement(2390..2749)
                     /locus_tag="VIX62_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858880.1"
                     /translation="MNTPTRIDFDELMRILMIVGFGALATWAMTAAVGVMSGFTYISG
                     SATLRFLLAVLILVFSRRTYWEIREWHWKKLNPDDRSGFANPLWEHGRSNEHLGEDTS
                     HEDAGFATATGSSQPRA"
     gene            2871..2957
                     /locus_tag="VIX62_01170"
     tRNA            2871..2957
                     /locus_tag="VIX62_01170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2905..2907,aa:Leu,seq:cag)
BASE COUNT          433 a         1030 c         1031 g          504 t
ORIGIN      
        1 ccggtgacgg ggcgcttccg ctgatcgacc gcgaacgcga cctcgctcac tgcccgcccg
       61 cgttcttcgg cttcttcggc gggaccgcgt cgtccgccac gtcggtccgg atccgctcga
      121 cgatggccag cgcgtcctcg cgactgtcgg cggtgatccc ttgcgggagg tcgcggtaga
      181 gctcgagggc gccggcatcg gcagcgggga tctcggtgat ggtgtcgacg ctcgacaggg
      241 acagactcag cggcttggcc gggatgccct ggtagacggc gaccttgtcg tcagcgacgc
      301 cgacgtacca ctggcggtcg atgaaggttc gggccgcgaa gaacgtcccc accagcagca
      361 ccagggccac caggccgccg atcagccagc gccgccagcg gggtgcatcc ggcgccgggc
      421 cgacgggatc gtcgtcctca ccgatcgcat ccagcacgac caccgtgatg ttgtcgatgc
      481 caccggcgcg gttcgccgcc ttgatgagac ggtcggacgc gcgctgcggg tcgggttcgg
      541 tctccaggat cgccttgatc tgatcctcgg tcaccatgcc ggtgaggccg tcgctgcaca
      601 gcaacaggcg gtcgccgtcc agcaggccca cgacgtcttc gtccacctgc acgtccgcct
      661 cggtgccgag cgagcgcgtg agcacgttgc ggtgcgggtg gacctcggct tcggcctcgg
      721 tgatctctcc ggtcttgacc atgcgggcca ccagcgtgtg gtcggcggtg agctgtcgaa
      781 gcgagtcggc tcgcagcaga taggcgcggg agtcgcccac gtgcgccagt tggagctggc
      841 tgccctccac cagggcggcg gtgagcgtcg tccccatgcc gctcacggcg gtgtcttcct
      901 gcgactgcat gaacacggcg tggttggcgc tgtgcacctg ctcgatgagc gagccttcgt
      961 ccttgcggaa cagatcttcg atcgtctcga gtgccagctg ggacgcgacc tcaccgcccc
     1021 gatgcccgcc catgccgtcg gccaccgcgt agagcggcgg ctcgatcagg taggagtcct
     1081 cgttgccctc gcggacccgg ccgatgtcgg tggccacccc gacctggacc ttcatcgtcg
     1141 gagctccatc acggtggtgc cgatccgcac cttgtcgcct gcctgcacca tggcggggga
     1201 ggacaggcgc tgatcgttca cgaacgtgcc gttggtcgaa cccaggtcct cgacgtacca
     1261 ggagccaccc ttggcgaaca cgcgggcatg ctgttgcgag acgtaggtgt cgtcgatcgc
     1321 gagctcgcac tcgggagccc ggccgatcac caacgaatcg ctcgccaccc ggacggtgcg
     1381 cggcttcgcg ccgtcggcat ggaccacgac gtcggagatc cccggcgtca ccgtgggacc
     1441 ctcggcggcg tcgcgagcct ttcgagccga acgtccaccg cgcgccggcg ccgcttcgac
     1501 gctgaggccg cggacggccc agcgcatcga cctccacagg aacaggacca gcagggcgaa
     1561 caggccgtac ttcagcgcgg acagggcgaa cggcgtcacc ccttcggcga cgaaggggat
     1621 ggcgggcggc gccaggctcg ccgtcaccgt cacgccctcc ggtactccag caccgtggtg
     1681 ccgatcgtga tgcggtcgcc gtcggagagc gctcccgact ggaccgtctg gccgttcagg
     1741 cgcgtgccgt tcgtcgatcc gaggtcggtg agtgtggtga ccccttcgct ggtgcgcaac
     1801 tgcacgtgac gccgcgaggc ccccttgtcc ttgagcacta cgtcgcaatc gctcatccgc
     1861 ccgatcgtga cgacctcctt ggcgatgggc acccgtcgct cggcgccgtt ctcgcggatc
     1921 aggagcatcg cggaggcgga gggggcggac gatggcgcgg gttcgtcggg cccctgcacc
     1981 aaggtggcca cgcaggtgat cgccccgcgc ttcagggatg cgtccgtggt gaaggtgatc
     2041 tccggcggcc cgaccagccc ccagccgcgt tcatcggcgt tctcgcgggc cacctgcgcg
     2101 agctcgctcg ccagggcggt ctcggcctgt tcgaaccgtt cggcatcctc ggtggagagc
     2161 gcgaactcga agtggttcgg ggcccagacg ccgttggggc ccacggtgcg ttcggcgtcc
     2221 atctccttga tgagtcgctt ggcgagctcc acgggttgga cgccgcttcg gaacgtcttg
     2281 gagaacaggc cttcgacgag gccccccaag cgtcgttcga agtcgcggag gttcggcacc
     2341 ggtagcgtgt ggcaggaggg ggacgaccct cccgcgcccg gcgcctatcc tacgcccggg
     2401 gctgtgacga gcctgtggcg gtcgcgaagc cggcgtcctc gtgggaagtg tcctcaccga
     2461 ggtgctcgtt gctgcggccg tgttcccaca acgggttggc gaagccggac cggtcgtcgg
     2521 ggttgagctt cttccagtgc cactcgcgga tctcccaata ggtgcggcgc gagaacacca
     2581 ggatcagcac cgccagcagg aaccggagcg tggccgatcc ggagatgtag gtgaaccccg
     2641 acatcacgcc gacggcggcg gtcatggccc acgtggcgag cgcaccgaac cccacgatca
     2701 tcaggatccg catcagttcg tcgaagtcga tcctggttgg cgtgttcatc tcgactctta
     2761 tcggcggcta cccgtttccg ggtagatggg ccgaacgggt ggtttcctgt gtccctcctc
     2821 cgggggacgg ctggcgaagg accgagcttg ctaccatcgc tgtcccactc gggcgagtgg
     2881 cggaattggc agacgcgcag gattcaggtt cctgtgtccg agaggacgtg ggggttcaac
     2941 tcccccctcg cccaccaccg atcgacggcc cctcctcgat ggctcaccac agcaaacg
//
LOCUS       DATDBZ010000046         5878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_422296, whole genome shotgun sequence.
ACCESSION   DATDBZ010000046 DATDBZ010000000
VERSION     DATDBZ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422296"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VIX62_01175"
     CDS             complement(<1..751)
                     /locus_tag="VIX62_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEY5858881.1"
                     /translation="MPRPGFVLEVDERTPPLLVHQGEGFRMQKFPLGTRVVYPPDALP
                     GIRDVGAAVQHALMHPLGDSKPLTELLFPGMKLTIAIDDISIPLPPMRTPDVRQRILE
                     PVIELAARKGVEDLELIIATALHRRMTSAEIRRAVGERVFRSFWPDQLYNFDAEDPQA
                     LLEIGTTDQGELVEISKRAAESDLIVYVNINLVAMDGGHKSVAVGLAGYRSLKHHHNV
                     NTMLHSKSYMDPREGHRAINDSVNRMGRVLAD"
     gene            complement(779..1987)
                     /locus_tag="VIX62_01180"
     CDS             complement(779..1987)
                     /locus_tag="VIX62_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY5858882.1"
                     /translation="MAQSLEYFRSVSRVLTARAVGSRAPGLIAGPLAPLRLVNRSDPE
                     ASRDGWARMKPILSGICGSDLSTIAGHSSFYFSPLVSLPFVPGHEVVGELLDDCGEHK
                     VGQRVVISSVLGCAARGLPDLCPNCEAGDYGRCDRVTVGDLKPGLQTGYCADTGGGWS
                     RMMLVHRSQIWAVPSEMDDRAAVLVEPLACAIHSVFRANVPDGADVMVIGAGTVGILT
                     LIALRRFANPGRVISAAKHTRQRGAAQMAGADEVVRPEHALKAVRRTESAVKLTPERG
                     QDFLLGGADVVFECTGSKSALDMALRTTKAGGRVVVSGIPGPGADLTPLWFRELELVG
                     AYTSGMETVGDGRRHSFALALEIASQLGPVLDQLVGATYPLDRWREAIDHAMSAGRLG
                     TFKVAFAPQD"
     gene            complement(1996..3072)
                     /locus_tag="VIX62_01185"
     CDS             complement(1996..3072)
                     /locus_tag="VIX62_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY5858883.1"
                     /translation="MLRRAERFTDIAEQVVTHMPKSRAMRNSVRRVDRDKARVDFVKR
                     YSDLYGMYTETEVVYTDDRTFALFESMSDEDKAMFPFDAAMVDWKYYLKDVHSPAVTQ
                     SLRELSKRDRERPTVKLREREEPVLAVFDMEGTIITSNVVESYVWTRMADLPSEEWPA
                     ELASVFGKVPRYLQIDRRDRGDFLRTFFRRYEGASVEGIDRLVANHVGEFMLQKASSA
                     AIRRIRDHRAAGHRTVLITAAAEPFIRPLAPLFDVLIGAELEVRDGRYTGFMSQPPLV
                     GEARSAWLKRYALLEGIDLKYSYAYADSYSDLPLLRAVGNPVAVAPDAALYRHARRRR
                     WPIEEWGMTKGMPRVRFPKPAVRG"
     gene            complement(3286..4299)
                     /locus_tag="VIX62_01190"
                     /pseudo
     CDS             complement(3286..4299)
                     /locus_tag="VIX62_01190"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(4379..5086)
                     /locus_tag="VIX62_01195"
     CDS             complement(4379..5086)
                     /locus_tag="VIX62_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5858884.1"
                     /translation="MSQRRPANGRWLFDQLVGEVRAGTVKARMRPFAKGEVVFHEDDP
                     GDTIHLIKKGLFAVRTSTSGRSLIINVLAPGDIFGEFALFSPGGRRTSDVTALIEGET
                     VAVERDALRAALIARPELVEGLMSSVVVKAESTRRRLVELLSISADLRVLRALLFVDT
                     LSHDDAPVPLTQQDLSSLAATTRPTANRVLREEAARGTLVLSRGRVTVVDPQALAKRA
                     GVELPLALANYVPFRPP"
     gene            5147..5434
                     /locus_tag="VIX62_01200"
     CDS             5147..5434
                     /locus_tag="VIX62_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEY5858885.1"
                     /translation="MDLSELQHTMRTTYLERDAARGRDATFRWLTEEVGELAKAMRTG
                     DRENLQHEFGDVLAWLLSLANVEGVELEDAIARYAAGCPRCGTSPCGCPAA"
     gene            5431..>5878
                     /locus_tag="VIX62_01205"
     CDS             5431..>5878
                     /locus_tag="VIX62_01205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5858886.1"
                     /translation="MNAQIVEPSEAHREQIAGVLSTSINFPLERALLNAPLLKLDDIR
                     VALEGDRVVATAGEFRFDQWFGGRGLDCCGITRVGTLPERRADGLATACTDALLQRAY
                     ERGAPLAALFPAVLRPYRRMGFEIAGSYTTHRLPLDAIAPDRDTLSV"
BASE COUNT          935 a         2101 c         1906 g          936 t
ORIGIN      
        1 tgtcggccag cacgcggccc atccggttca ccgagtcgtt gatcgcgcgg tggccctcac
       61 gcggatccat gtacgacttg gaatgcaaca tcgtgttcac gttgtggtgg tgcttcaggc
      121 tgcggtagcc ggccagaccc accgccacgg acttgtgtcc gccgtccatg gcgaccaggt
      181 tgatgttgac gtagacgatc aggtcggact ccgcggcgcg cttggagatc tcgaccagtt
      241 ctccttggtc ggtggtgccg atctcgagca gcgcctgcgg gtcctccgcg tcgaagttgt
      301 atagctggtc gggccagaac gagcggaaca cgcgctcgcc caccgcccgg cggatctccg
      361 cgctggtcat ccggcggtgc agcgccgtgg cgatgatcag ctcgaggtcc tcgacgccct
      421 tgcgcgccgc gagttcgatc accggctcca ggatccgctg ccgcacgtcg ggggtgcgca
      481 tcggcggcag cgggatcgag atgtcgtcga tcgcgatcgt gagcttcatc ccggggaaca
      541 gcagctcggt gagcggcttc gagtcgccca gcggatgcat cagcgcgtgt tgcaccgcgg
      601 cgcccacgtc gcggatcccc ggcagcgcgt cgggcgggta caccacgcgc gtccccagcg
      661 ggaacttctg catgcggaag ccctcgccct ggtgcaccaa cagcgggggc gtccgctcgt
      721 ccacctccaa cacgaatcct ggtcgtggca tcgtctgtcg tctcgctctc tcttgtgtct
      781 agtcctgcgg cgcgaacgcc actttgaacg tccccagccg gccggccgac atcgcgtggt
      841 cgatcgcctc gcgccatcgg tcgagcgggt acgtcgcgcc caccaactgg tccagcaccg
      901 gccccagctg cgacgcgatc tccaacgcga gcgcgaacga atgccgccgt ccgtcgccca
      961 cggtctccat gccgctcgtg tacgcgccca ccaactccag ctcgcggaac cacagcggcg
     1021 tcagatcggc gcccggcccc gggatgccgc tcaccaccac gcggccgccc gctttggtcg
     1081 tgcgcaaggc catgtcgagc gcgctcttgg agcccgtgca ctcgaacacg acgtcggccc
     1141 cacccagcag gaagtcctgc ccccgctcgg gcgtgagctt caccgccgat tccgtgcggc
     1201 gcaccgcctt cagggcgtgt tcgggacgaa ccacctcgtc ggcaccggcc atctgcgcgg
     1261 ccccccgttg gcgcgtgtgc ttggccgccg agatcacccg gcccgggttg gcgaaccgcc
     1321 gaagcgcgat caacgtcagg atgcccaccg tgcccgctcc gatcaccatc acgtcggccc
     1381 cgtcgggcac gttcgcgcgg aacaccgaat ggatcgcgca cgccagtggc tccaccagca
     1441 ccgcggcccg gtcgtccatc tccgagggga ccgcccagat ctgcgaacga tgcaccagca
     1501 tcatccggct ccagccaccg ccggtgtcgg cgcagtaccc ggtctgcagg cccggcttca
     1561 gatcgcccac cgtgacccgg tcgcagcggc cgtagtcgcc ggcctcgcag ttcggacaca
     1621 ggtccggaag cccccgcgcc gcgcacccca gcacgctcga gatcacgacg cgctgcccga
     1681 ccttgtgctc accgcaatca tcgagcagtt cgcccacgac ctcgtggccc ggcacgaagg
     1741 gcagcgacac cagcggcgag aagtagaacg acgaatgccc tgcgatcgtg gacaggtcgc
     1801 tgccgcagat gcccgacagg atcggtttca tgcgggccca tccgtcgcgg gacgcctcgg
     1861 ggtcggaccg attcactaag cgcagcggcg cgagcggacc ggcgatcagg ccgggcgcgc
     1921 gggaacctac cgcgcgcgcg gtcaacaccc gcgagacgga acggaagtac tcgagcgatt
     1981 gcgccatcta cgcgctcacc cccgcaccgc cggcttcggg aatcgcaccc gcggcatccc
     2041 cttggtcatg ccccactcct cgatcggcca gcgccgacgt cgcgcgtgtc gatagagcgc
     2101 cgcatcgggc gccaccgcga ccgggttgcc cacggcacgc agcaacggca ggtcggaata
     2161 ggaatccgca taggcgtacg agtacttcag gtcgatcccc tcgagcaggg cgtaccgctt
     2221 gagccaggcg ctgcgtgcct cgcccaccag cggtggctgc gacatgaacc cggtgtagcg
     2281 cccgtcgcgc acctccagct ccgccccgat cagcacgtcg aacaacggcg ccagcggacg
     2341 gatgaacggc tccgctgccg cggtgatcaa gactgtgcga tgcccggcgg cgcggtggtc
     2401 gcggatgcgc cggatcgccg cggagctggc cttctgcagc atgaactcgc cgacgtggtt
     2461 cgccaccagg cggtcgatcc cctcgacgct cgcgccctcg taccggcgga agaacgtgcg
     2521 caggaagtcc ccgcggtcgc gccggtcgat ctgcaggtac cgcggcacct tgccgaacac
     2581 gctggcgagc tccgcgggcc actcctccga cggaagatcc gccatccgcg tccacacgta
     2641 ggactccacc acgttcgacg tgatgatcgt gccctccatg tcgaacacgg cgagcacggg
     2701 ttcctcgcgc tctcgcagct tcacggtggg ccgttcgcgg tcgcgcttgc tcagctcgcg
     2761 caggctctgc gtcaccgccg gggagtgcac gtccttcagg tagtacttcc aatcgaccat
     2821 ggcggcatcg aacgggaaca tcgccttgtc ctcatcgctc atggactcga acaacgcgaa
     2881 cgtgcggtcg tcggtgtaga ccacctcggt ctcggtgtac atgccgtaca ggtcgctgta
     2941 gcgcttcacg aagtcgacgc gggccttgtc gcggtcgacg cggcgcaccg agttccgcat
     3001 cgcccttgac ttgggcatgt gggtgaccac ctgctcggcg atatcggtga accgctccgc
     3061 acgccgcagc atccgttcga ccgtgaggtt gcccgggaag tcccaatcgg gaaccttgtg
     3121 ctcgcctcgg ccacgttcgg gcagcgggtg ctcttcgaag tagccgcgca cccattcgta
     3181 caactcgaag aaccgcaggg ggttccgcga acccgagctc acgttgtaat gcgcggcctc
     3241 gccggcggcg ggtgggttgg cggcgaccgc caggatcgcg ttcaccacga agtcgaccgg
     3301 gatgatgtcg atgatgcctt cggggatccc agggaactcg gggatctggc ccagcccgta
     3361 tgcgcggatg atcgggtcag ccatcttgaa cccgtcgatc cacccgggga acggatgggt
     3421 gtaggccgac tcgatgatcg agggccgcac gatgctgagc ggcaggttcg cctcagcggc
     3481 gagctcctcg accgcgcgct cgcccatcgc cttggtgaac gtgtagacgt cgggccagcc
     3541 caacgaacgg gcccgcgcgc gtccgtactc aaccagccgc ttcgtgaccc acttctgccg
     3601 gcgctcctcg gcgtcggtcg ccacggtcga ggggccggcg cgctcgtgct ccttccgcgc
     3661 ggccgcgagg aacgcgttca gcatctcggg cttgcgggac tgcgcctcca cgtcatggcg
     3721 ggcttccatc gcgagctcca gctccaagcg atagtccacc cggtgatcga gcggaccttc
     3781 cgggatcacg cccttctgca cgccggcgac gtaggccgtg gacacatgga cgagcgccgg
     3841 ggtggatccg ccggcgatca ctccgccgta gaggttcatc gcaccctgca ggttggtggt
     3901 gaagccctcg tcgatcggtg ggtcgaacgc gaccgtggcc gccgagtggc aggcgatgtc
     3961 gatgccaccg gggatctcgg gcacgccacg tccgaagtcc gcgtcgatca cgtccacacg
     4021 ttcgtcaagt accttgacca actactcctg gcccacacgt tcacgcagcg tgtcgaacgc
     4081 cggcttgcgc agcaggtact cgacccgctc tcggctggtg gcgccggtct gcgagcgcac
     4141 caacaggatg acgcgggtct cgtggaagtc ggccatgagc cgttcgaaca cgacctgggc
     4201 caggaatccg gtgacgccgg tcagcaggat cgtcttgccg gacagcgcct ccacgatcga
     4261 ggagtcgctt cctcgggcct ccgccccgtt ctctaccatg cggcagatta tgccctctct
     4321 accacttggt agacaagtcg agaggccatg cccactctgg cccgacgccc cagctgtcct
     4381 acgggggcct gaagggaacg tagttggcga gggcgagggg aagttccacg ccggcacgct
     4441 tggcgagcgc ctgagggtcg accaccgtga cgcgccctcg agacagcacc agcgtccccc
     4501 gcgccgcctc ttcccgaaga acacgattcg ccgtgggacg tgtcgtcgcg gctaagctcg
     4561 acagatcctg ttgtgtcagc ggtacgggtg cgtcgtcgtg gctgagtgtg tcgacgaaca
     4621 gcagcgcgcg gaggacccgg aggtccgccg agatcgagag caactcgacg agccggcgcc
     4681 gagtgctctc cgccttgacg acgaccgagg acatcaggcc ttcgaccagt tccggcctgg
     4741 cgatgagcgc tgctcggagc gcgtcgcgtt cgaccgcgac ggtctcgccc tcgatgagcg
     4801 cagtgacgtc gctggtcctt ctgccgcccg gtgagaagag cgcgaactct ccgaagatgt
     4861 cgcccggcgc gaggacgttg atgatgaggc tccgcccgct ggtggacgtc cgcacggcga
     4921 acagcccctt cttgatcagg tggatcgtgt cgcccggatc gtcctcgtgg aagacgacct
     4981 cccccttggc gaacgggcgc atgcgggcct tgaccgttcc ggctcgtact tcacccacca
     5041 gctgatcgaa cagccaccga ccgttggcgg gtctccgctg cgacatcggg cacagtgtga
     5101 cggtcggtga acatcggcgc aacggcgacg cgcctagact ctcccgatgg acctgtccga
     5161 actgcaacac acgatgcgca ccacctacct cgagcgagac gctgctcggg gccgcgacgc
     5221 cacgttccgc tggctcaccg aggaggtggg cgaactcgcg aaagcgatgc gcaccggcga
     5281 ccgggagaac ctccagcacg agttcggtga cgtactcgcc tggctgctgt cgctcgccaa
     5341 cgtcgagggt gtcgaactcg aggacgcgat cgcccgctac gccgccggct gcccgcgatg
     5401 cggcacgtcc ccgtgcgggt gcccagccgc atgaacgcac agatcgtcga gcccagcgaa
     5461 gcccaccgcg aacagatcgc gggtgtgctg tcgacgtcga tcaacttccc gctcgaacgc
     5521 gccctgctca atgcgccgct gctcaagctc gacgacatcc gcgtcgcact cgaaggcgat
     5581 cgcgtggtcg ccaccgccgg agagttccgg ttcgatcagt ggttcggcgg acgcggcctc
     5641 gactgctgtg ggatcacccg cgtgggcacg ttgcctgagc gtcgagcgga cggcctcgcc
     5701 accgcgtgca ccgatgcgct gctccaacgg gcgtatgaac gaggggcgcc gctcgcggcg
     5761 ctcttccccg cggtactacg gccgtaccga cggatggggt tcgagatcgc ggggtcatac
     5821 accacgcacc gactgcccct cgacgcgatc gcccccgacc gcgacacgct gagcgtcg
//
LOCUS       DATDBZ010000047         6557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2471176, whole genome shotgun sequence.
ACCESSION   DATDBZ010000047 DATDBZ010000000
VERSION     DATDBZ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471176"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VIX62_01210"
     CDS             <1..329
                     /locus_tag="VIX62_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858887.1"
                     /translation="DRTTRQIVRSVRAQVPSTAFVVTSTGSLRGARGEDAAAIAQRVD
                     ASLGSQVIEASAADGLFIDRTVATQASITAAGVSDAMEAERTPAGSPVFAGTFPSFAV
                     AFARYC"
     gene            329..1351
                     /locus_tag="VIX62_01215"
     CDS             329..1351
                     /locus_tag="VIX62_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY5858888.1"
                     /translation="MAVDVARRPGATEPRRGSASSRAGLWVLTGLAGIAILVRLLDPE
                     QFAWVRNFLIVFGSLLVEAMPFIVLGAFVSAAIEVFVPASAFEKIARLPRGLQIPAAA
                     ASGMAFPVCECGSVPVARRLAARGLAPSAAVTFMLAAPIVNPVVVASTYVAYRGRDTV
                     AIMVLGRLALGFIVAMAVGWVLGAVRGDQLLKQRDAEVEAHEVDAAERRWRRFFGHLT
                     GDIVFMARFLILGAAIAAAVQTFIPQSILNGVANLPILSLIAMMALAFIMSLCSESDA
                     FVAASFTAFGPAPQLAFLVFGPMVDLKLGALYVGTYERGFFRMVVIAVGSTTLAATLW
                     IQVFFG"
     gene            1348..2043
                     /locus_tag="VIX62_01220"
     CDS             1348..2043
                     /locus_tag="VIX62_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEY5858889.1"
                     /translation="MKRHWSPARVGAGLVIGAWAGLFWFVWAAGRTSLYLSTRTAWLI
                     PMGAILMTVAAVGRLASARVERTEHLGSRETVMFGVIALPVVLLLALPPATLGSYAAG
                     RRSNSTGIAASARIVSGPIDFVDIAAARSVKSAAADLRARAGERVTMDGFVTVDEGDT
                     DRFQLTRFVITCCVADATISYVTVVDAPPGQVANDDWVRVTGTIYPLGSDILVDAETV
                     VPIPPPDDPYLTP"
     gene            complement(2033..2491)
                     /locus_tag="VIX62_01225"
     CDS             complement(2033..2491)
                     /locus_tag="VIX62_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY5858890.1"
                     /translation="MTASANDLHATVEASLRSVGQRYTPKRRALVTLLERADKPLSMS
                     DLLAGKTGLPQSSIYRNLAVLEQAGAVRRVITEDEFARFELTEALTEHHHHLICSNCG
                     KVEDVTIPADLESSMDRTIDRVARRAGFAHVSHRLDLIGTCRSCADAATA"
     gene            complement(2488..3411)
                     /locus_tag="VIX62_01230"
     CDS             complement(2488..3411)
                     /locus_tag="VIX62_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY5858891.1"
                     /translation="MIALTLPYPFDLHFMQRALIAGVAVGVFAPMIGTFLVQKRMSLI
                     GDGIGHIAFAGVGAGFLAQSGVIAGVALGLGPVWMALVFAVAGSLGIEWLRSRRRASG
                     DLALALFFYSGIALGVVLMSLGGGLNASVLTYLFGQPLTVSAQEVVLIVSLGAAIVVA
                     MLVLRRVLFAVVTDEDWSNVAGLPVGLVNNVLAVITAVAVVAAMQIVGILLIAAMMVL
                     PVASGQLLARSFAGTMRWAVAIGVASVVAGLAASRIWDLAPGGTIVLLAAGVFMVVAL
                     VTRRVGGRVPMIEPYAPDEPGAPSHEGAHAG"
     gene            complement(3408..4187)
                     /locus_tag="VIX62_01235"
     CDS             complement(3408..4187)
                     /locus_tag="VIX62_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEY5858892.1"
                     /translation="MTVAEPARRPITASLLRADGVRFAYGRSPVLEDVSLDVAPGEFV
                     ALVGPNGSGKSTLLKVLLGSLIPDRGIVRLFGEDPVAFRDRGRLGYVPQRPSLASEVP
                     ATVAEIVAAGRLSRRGWWRPLSGSDRTAVSHALESVGLADLADRPINQLSGGQQQRAF
                     IARAFASEPSLLVLDEPIAGVDAESQRRFRDAIVHLIAEHGSGVLLVSHELSAVAADL
                     DRVVVLKRRILFDGPPSQLTAEGVSLGVHREDLPLWLEGLT"
     gene            complement(4174..5085)
                     /locus_tag="VIX62_01240"
     CDS             complement(4174..5085)
                     /locus_tag="VIX62_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY5858893.1"
                     /translation="MNPRRAVGVVAAMLMAALPACAETGSSASEADVSIAASFYPLAY
                     AAEQVGGACVQVTNLTPPGTEPHDLELTPDDVESIATADLVVYLGGGFQPAVQDAIDE
                     AQGATLDLLAEVDTASAPPDEADEGLAVDPHVWLDPGLFATAIPPLVDAMESAATDAA
                     CDFRANGAALIEELDGLDAEFVAGLADCEVTTLVTSHAAFGYLAAAYGLTQAAISGLA
                     PDTEPTPDRIAELVDLVHRDGVTTVFSEALVSPEVAQTLADEAGVQVAVLDTLEGLTP
                     DEVDAGQDYGSVMRRNLETLRGGLDCR"
     gene            5199..6239
                     /locus_tag="VIX62_01245"
     CDS             5199..6239
                     /locus_tag="VIX62_01245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5858894.1"
                     /translation="MVVRVGIGLFTGQVPAGSPRSFAQEYRETLELVRLAEALGFDSA
                     WVSEHHGSSDGYLPSLMVMLGAFADATSHIRLGTGVILTPFHDPIRLAEDAAVVDQLS
                     DGRLMLGLGIGWREEEFRMFGVPIAERVPRLVDTVAILRRAWTGERFSYEGEVLSCDR
                     VRVTPPPAQAGGPPILLGGYVDAAMRRAGRIGDGHITDADDAGQVQHAVSLMDEGARE
                     AGRDPSSSHLALMVNAFVADDGEQAWDLARNGVLHQLGAYEAWDAGHDTPEHDSLETD
                     GADDHAARRGTAAGTPEDVVHALRPLVATHGSRKEFDVIVRLHYPGMAFDTAARAVEL
                     FGERVLPALKGE"
     gene            complement(6251..>6557)
                     /locus_tag="VIX62_01250"
     CDS             complement(6251..>6557)
                     /locus_tag="VIX62_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858895.1"
                     /translation="QPWCRFAAAGSAGTRERRATTRRQAPFEGSMRQVRGSVVALLRE
                     RPSVTIAAAVSATGHTPARITEAVVGLSHDGVIDASPAALRGSTRGTIKLAEDTSVN"
BASE COUNT          973 a         2307 c         2300 g          977 t
ORIGIN      
        1 tggatcgcac gacccgccag atcgttcgct ccgtccgggc gcaggttccc tcgacggcgt
       61 tcgtggtgac gtcgaccggg agccttcgtg gcgcccgcgg tgaggacgcc gcggcgatcg
      121 cacaacgggt ggacgcgtcg ctggggagtc aggtgatcga ggccagcgcg gcagacggac
      181 tgttcatcga tcggacggtg gcgacgcagg catcgatcac ggcggccggc gtctcggacg
      241 cgatggaagc cgaacgcaca cccgccggca gcccggtgtt cgccggtacc ttcccgtcgt
      301 tcgccgtggc gttcgcgagg tactgctgat ggccgtcgac gtcgcgcgcc ggccgggcgc
      361 caccgagcct cgccgcgggt ccgcatcatc acgcgcgggc ctgtgggtgc tcaccgggct
      421 cgccgggatc gcgatcctcg tccggctcct cgatcccgag cagttcgcct gggtgcgcaa
      481 tttcctgatc gtgttcggat cgctgctggt cgaggcgatg ccgttcatcg tgctgggcgc
      541 gttcgtctcg gcggcgatcg aggtcttcgt gccggcgtcg gcgttcgaga agatcgcccg
      601 tctcccccgc ggtctgcaga tcccggcggc agcggcctcg ggcatggcct tccccgtgtg
      661 cgaatgcggc tcggtgccgg tggcccggag gctcgccgcg agagggctgg cacccagcgc
      721 cgcggtgacg ttcatgctgg ccgccccgat cgtgaacccg gtcgtcgtgg cctcgaccta
      781 cgtcgcctac cgcggccgtg acaccgtcgc gatcatggtc ctgggccggc tggcgctggg
      841 cttcatcgtg gcgatggcgg tcggctgggt gctgggcgcc gtgcgtggag accagctgtt
      901 gaagcagcgc gatgccgagg tcgaggcgca cgaggtcgac gcggccgagc gccggtggcg
      961 acggttcttc ggacacctca cgggtgacat cgtgttcatg gccaggttcc tgatcctcgg
     1021 cgcggcgatc gccgcagcgg tgcagacctt catcccgcag tcgatcctca acggcgtggc
     1081 caatctgccg atcctgagcc tcatcgcgat gatggcgctg gcgttcatca tgtcgctctg
     1141 ctcggagtcg gatgccttcg tggcggcctc gttcaccgcg ttcggcccgg ccccgcagct
     1201 ggcgttcctg gtgttcgggc ccatggtcga cctgaagctc ggtgccctct acgtcgggac
     1261 ctacgaacgc gggttcttcc ggatggtggt gatcgccgta ggatccacga cgcttgcggc
     1321 gaccctgtgg atccaggtgt tcttcggatg aagcggcact ggagtcccgc tcgcgtcggc
     1381 gccggcttgg tgatcggggc ctgggccgga ctgttctggt tcgtgtgggc ggcgggccgc
     1441 acgtcgctgt acctgtcgac gcgcaccgca tggctgatcc ccatgggggc gatcttgatg
     1501 acggtcgcgg ccgtgggccg cctggcgagc gcgcgcgtgg aacgcaccga gcacctgggc
     1561 tcccgcgaga ccgtgatgtt cggcgtgatc gccctgccgg tggtgctgct gttggcgttg
     1621 cccccggcca ccttgggctc ctacgccgcg ggccggaggt cgaactccac cgggatcgcg
     1681 gcgagcgccc gcatcgtttc gggtccgatc gacttcgtcg acatcgccgc cgcccgctcc
     1741 gtgaagtccg cggccgccga cctccgggcc cgcgccggcg agcgggtcac gatggacggg
     1801 ttcgtcaccg tggacgaggg cgacaccgac cggttccagc tcacgaggtt cgtcatcacc
     1861 tgctgcgtgg ccgacgccac gatctcctac gtcaccgtgg tggatgcccc gccgggccag
     1921 gtggcgaacg acgactgggt gcgggtgacg gggacgatct acccgctagg ttcggacatt
     1981 ctggtcgacg ccgagaccgt ggtgcccatc ccgccgccgg acgatcccta cctcacgccg
     2041 tagccgcgtc ggcgcagctg cggcaggtgc cgatcaggtc gagacgatgg ctcacgtgcg
     2101 cgaaccccgc ccgccgcgcg acgcggtcga tcgtgcgatc catcgaggac tccagatcgg
     2161 cagggatcgt gacgtcctcc accttgccgc agttcgaaca gatcaggtga tgatgatgct
     2221 cggtgagcgc ctcggtgagc tcgaaccgcg cgaactcgtc ctcggtgatc acgcgccgga
     2281 cggcgccggc ctgctcgagc accgcgaggt tgcggtagat cgagctctgc ggcagcccgg
     2341 tcttgcccgc cagcaggtcg ctcatcgaca gcggcttgtc ggcgcgttcc aggagcgtga
     2401 cgagcgcccg ccgcttcggc gtgtatcgct ggcccaccga gcgcaggctc gcctcgacgg
     2461 tggcgtggag gtcgttggcc gacgcggtca tccggcgtgc gctccctcgt gcgacggtgc
     2521 gccgggctca tccggggcgt acggctcgat catggggaca cgaccgccga cgcgccgggt
     2581 caccaacgcc accaccatga acactcccgc ggccagcaac acgatcgtgc cgcccggcgc
     2641 gaggtcccag atgcgcgaag ccgccagacc ggccaccacg gaggcgacac cgatcgccac
     2701 cgcccaccgc atcgtgccgg cgaacgaacg cgcgagcaat tgcccgctgg cgacgggcag
     2761 caccatcatc gcggcgatca acaggatgcc cacgatctgc atcgcggcga ccacggctac
     2821 cgcggtgatc accgcgagca cgttgttcac cagccccacc ggcagaccag cgacgttcga
     2881 ccaatcctca tcggtcacga ccgcgaagag cacccgccgc agcaccagca tcgccaccac
     2941 gatggccgcg cccagcgaca cgatcagcac gacctcctgc gcgctcaccg tgagcggttg
     3001 accgaacagg taggtcagga cgctcgcgtt cagcccgccg ccgagactca tcagcaccac
     3061 cccgagcgcg atgcccgaat agaagaacag cgcgagcgcg aggtcaccgg acgcccggcg
     3121 tcgtgaccgc agccactcga tgccgagcga tccggccacc gcgaacacga gggccatcca
     3181 caccgggccg agcccgagcg ccacgccggc gatgaccccc gactgcgcga ggaatcccgc
     3241 accgaccccg gcgaacgcga tgtggccgat gccgtcaccg atcaaggaca tgcgcttctg
     3301 caccaggaac gtgccgatca tcggcgcgaa cacccccacc gccacgccgg cgatcaatgc
     3361 gcgctgcatg aaatgcagat cgaacggata cggcagggtc agcgcgatca cgtgagccct
     3421 tccagccaca agggcaggtc ctcacggtgc acgcccaggc tcacgccctc ggcggtgagc
     3481 tgcgacggtg gcccgtcgaa caggatgcgt cgcttcaaca cgacgacccg gtcgagatcg
     3541 gccgcaacgg ccgacagttc gtgggagacc aggagcacgc cggaaccgtg ttcggcgatc
     3601 agatgcacga tcgcgtcgcg gaaccgacgt tgggactccg cgtcgacgcc ggcgatgggc
     3661 tcgtcgagca ccaacagcga tggctcgctc gcgaacgccc gggcgatgaa cgcccgctgc
     3721 tgctgaccgc ccgagagctg gttgatcggc cggtcggcga ggtcggcgag gccaaccgat
     3781 tccaacgcgt gggacaccgc ggtgcggtcg ctcccgctga gcggtcgcca ccagccgcgg
     3841 cgggacaggc ggccggcggc cacgatctcg gcgacggtgg ccggcacctc cgaggcgagc
     3901 gacgggcgtt gcggcacgta cccgagacgc ccgcgatcgc ggaaggccac cggatcctcg
     3961 ccgaacagcc gcacgatgcc gcgatcgggg atcagcgacc ccagcagcac cttgagcaac
     4021 gtcgacttgc ccgaaccgtt cggccccacg agcgccacga actcgccggg cgccacgtcg
     4081 agcgagacgt cctcgagcac gggactgcgg ccataggcga accggacgcc gtcggcccgc
     4141 aacagcgagg cggtgatcgg tcggcgggcc ggctcagcga cagtcaaggc cacctcgcag
     4201 ggtctcgagg ttgcggcgca tcacggaacc gtagtcctgg cccgcatcga cctcgtccgg
     4261 cgtcagccct tccagggtgt ccagcaccgc gacctggacg ccggcttcgt ccgccaacgt
     4321 ctgggccacc tccggcgaga ccaacgcttc gctgaagacc gtggtgacgc cgtcacgatg
     4381 cacgaggtcg acgagttcgg cgatccggtc gggcgtgggt tcggtgtcgg gcgcgagccc
     4441 ggagatcgcc gcttgcgtga ggccgtaggc ggcggcgagg tagccgaacg cggcgtggct
     4501 cgtgaccagg gtcgtgacct cgcagtcggc aaggcccgcg acgaactcgg cgtcgaggcc
     4561 atccaactcc tcgatcagcg cggccccgtt ggcacggaaa tcgcacgcgg cgtcggtagc
     4621 cgcggactcc atggcgtcga ccagtggcgg gatcgccgtc gcgaacagac cgggatcgag
     4681 ccagacatgg gggtcgaccg cgagtccctc gtcggcctca tccggcggcg ccgaggcggt
     4741 gtcgacctcg gcgagcaggt cgagcgtggc gccctgggcc tcatcgatcg catcctggac
     4801 cgcgggctgg aacccgcctc ccaggtagac caccagatcg gcggtggcga tcgactcgac
     4861 gtcgtcgggc gtcagctcga ggtcgtgtgg ctcggtgccc ggcggagtga ggttcgtcac
     4921 ctggacgcag gcgccgccca cctgttccgc cgcatacgcg agcggataga agctcgcagc
     4981 gatggagacg tcagcttcgc tcgccgagga acccgtttcc gcgcaggccg gaagcgcggc
     5041 catcagcatg gcggccacga cacccacggc acggcgggga ttcatgtctc tagttctaag
     5101 aatcggtctc attctcggac ctcacggtag catcgcgggt ggtccgggga caacggctgg
     5161 ggtggaggac cgctggtgtc gtgcggtagc gtgctccgat ggtcgtccgg gtggggatcg
     5221 gactgttcac cggccaggtg ccggcaggat cgccacggtc gttcgcgcag gagtaccgcg
     5281 agacgctgga gctggtgcga ctggccgaag cgttggggtt cgactcggcg tgggtcagcg
     5341 agcaccacgg ttcttccgac ggctacctgc cttcgctcat ggtgatgctg ggggcgttcg
     5401 cggacgccac gagccacatc cggctgggca cgggcgtgat cctcaccccg ttccacgatc
     5461 cgatccggct cgccgaggat gcggccgtgg tcgatcagct ctccgacgga cggctcatgc
     5521 tcggcctggg catcggctgg cgcgaggagg agttccggat gttcggcgtc ccgatcgcag
     5581 aacgggtccc gcgcctggtc gataccgtcg cgatcctgcg gcgcgcctgg acgggcgagc
     5641 gcttctccta cgagggcgag gtgctctcct gcgaccgggt gcgcgtgacg ccgccgcccg
     5701 cacaagccgg tggtccgccg atcctgctgg gcggctacgt cgacgccgcc atgcgtcgcg
     5761 ccggccggat cggtgacggg cacatcaccg acgccgacga tgccggccag gtgcagcacg
     5821 ccgtctcctt gatggacgaa ggtgcgagag aggccgggcg cgatccctcc tcgtcgcatc
     5881 tggccctgat ggtcaacgcc ttcgtcgccg atgacgggga acaggcgtgg gacctggccc
     5941 ggaacggggt gctccatcag ctgggcgcgt acgaggcctg ggacgccggc cacgacaccc
     6001 cggagcacga ttccctggag accgacggcg cggacgacca cgccgcccgt cgcggcaccg
     6061 cggccggcac gccagaggac gtcgtccacg cgctgcgacc gctggttgcc acccacgggt
     6121 cgcgcaagga gttcgacgtg atcgttcgcc tccactaccc gggcatggcc ttcgacaccg
     6181 cggcgcgcgc cgtcgagctg ttcggcgaac gcgtcctgcc cgccctcaag ggcgagtagt
     6241 ccgtggtgga ctagttgacg gacgtgtcct cggccagctt gatggtgccc cgcgtgctgc
     6301 cgcggagcgc cgcaggcgac gcatcgatca caccgtcgtg gctcagtccc accacggcct
     6361 cggtgatgcg ggccggggtg tgcccggtcg ccgacaccgc ggcggcgatc gtcaccgagg
     6421 gacgttcgcg gagcagcgcc accaccgaac cacgcacctg acgcatcgag ccctcgaacg
     6481 gagcctggcg ccgagtggtg gcccggcgct ccctggtccc ggccgaaccc gctgccgcga
     6541 accggcacca cggctgc
//
LOCUS       DATDBZ010000048         4399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2267125, whole genome shotgun sequence.
ACCESSION   DATDBZ010000048 DATDBZ010000000
VERSION     DATDBZ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267125"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VIX62_01255"
     CDS             complement(<1..167)
                     /locus_tag="VIX62_01255"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEY5858896.1"
                     /translation="MTTARQRVAGGILGLALGDALGAPFEFRRAHDIPSPIPAFELPW
                     MDRPPGSWTDDT"
     gene            360..962
                     /gene="rpsD"
                     /locus_tag="VIX62_01260"
     CDS             360..962
                     /gene="rpsD"
                     /locus_tag="VIX62_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018394170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY5858897.1"
                     /translation="MDKIGPKHRTCRRVGAPLCGRPNCPSNKRPYPPGQHGRGRKRIS
                     EYQTRLVEKQKLRAIYGVSESQMRRYYDEATRSKGVTGEEMIGRLETRLDTVVLRLGF
                     ALTTRQARQLVSHGHVTLDGKRLNIPSAKVKPGQTVEIAAKAKNFVAVREALEITADP
                     PPYLYRNKDEVSGTLSRAPERTEVPLPVEVEERLIVEYYS"
     gene            1043..2065
                     /gene="gnd"
                     /locus_tag="VIX62_01265"
     CDS             1043..2065
                     /gene="gnd"
                     /locus_tag="VIX62_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEY5858898.1"
                     /translation="MQLGMVGLGRMGANLSRRLMRAGHEVVAYDVSPEAVEGLADEGA
                     VGASSLEDFVAKLTAPRAAWIMVPAAFAGSTAEQLAALMETDDVIIDGGNSYYRDDMA
                     RSAAFAKRGIHYLDVGTSGGVFGLERGFCMMIGGDPAPVQRLDPIFSALAPGIETAER
                     TPGRAESGRGGTAEHGYLHCGPSGAGHFVKMVHNGIEYGLMAAFAEGLNVLRHANVGA
                     ADREDDAETAPLREPEFYRYDLDIADVAEVWRRGSVVSSWLLDLTASALVNDPTLEGF
                     TGRVSDSGEGRWTLQAAIDEGVPAQVLSAAVFDRFASQGSAEFANKVLSAMRKEFGGH
                     DEKAST"
     gene            2062..3714
                     /gene="pgi"
                     /locus_tag="VIX62_01270"
     CDS             2062..3714
                     /gene="pgi"
                     /locus_tag="VIX62_01270"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY5858899.1"
                     /translation="MSRTAVDGSPAWQALADHAVANRSTTLRDLFAAEGDRGNRLTAE
                     AGDLYLDYSKHLVTDETLALLLQLAQDAGVAGRRDAMFAGEHINVTEDRAVLHVALRM
                     PRDATLVVDGVDVVAEVHEVLGRMGAFADRVRSGEWLGATGERITTVVNIGIGGSDLG
                     PSMAYEALLDVSQRDIGCRFVSNVDGADIAEATRDLDPARTLFVISSKTFTTLETLTN
                     AHSAREWLVASLGEDAVANHFVAVSTNAEGVTTFGIDPENMFGFWDWVGGRYSMDSAI
                     GLSLMIAIGPEAFADMLAGFHTIDEHFRTAPFERNLPVLQGVLGIWYRNFLGAQTKAI
                     LPYSHHLSKLPAYLQQLDMESNGKSVTLDGEPVGYDTGPIVWGTPGTNGQHAYYQLIH
                     QGTTLIPADFIGFAHATDPLGDHHDLLMANVFAQTEALAFGKTREEVEAEGVPAHQVP
                     HRTFHGNHPTSTILAPSLTPNTLGQLIALYEHTVFVQGTIWDIDSFDQWGVELGKVLA
                     GRIATELAAPSDPDAGAHDSSTMALISRYRRLRDDDPEGTSR"
     gene            3711..>4399
                     /locus_tag="VIX62_01275"
     CDS             3711..>4399
                     /locus_tag="VIX62_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY5858900.1"
                     /translation="MTLQPPIPQAIVVFGASGDLAKKKILPAIYNLAVDGLLPPAYEV
                     IGFASSDMDDERFRQHARDAIEEHSRRPIDEAVWNALAPKFRYIMGSFEDPEAMKRLG
                     EVLDEADRVHGCDGCRLFYMAVPPTLFPVIARSLDAAGLNSELGRLVVEKPYGDSLES
                     AKALTNALYEGFTEEQIFRIDHYLGKETVQNLVVFRFANSIWERVWNRDAVDSVQLTV
                     AESIGVEGRAG"
BASE COUNT          734 a         1497 c         1489 g          679 t
ORIGIN      
        1 gtgtcgtcgg tccaggaacc cggcgggcga tccatccacg gcagctcgaa tgccggtatc
       61 ggcgacggga tgtcgtgcgc ccggcggaac tcgaacggcg cccccaacgc gtccccgagc
      121 gcaagtccga ggatgccgcc ggcgacccgt tgcctcgccg tggtcatcga gggagcctac
      181 tccggggttc cgtggggcgt tcctgcaggt caactggtat cttcggtggg catcagggca
      241 cgcgttctcg caggtcgcat gccacatccg gcgacacttc acggtcaggg gtcctccagt
      301 cccacgccct gaccagcggg aacacccgac ggactggacg aactgaggga tcgacgcaca
      361 tggacaagat cggaccgaag caccgcacct gccggcgggt cggcgcgccg ctctgcggcc
      421 gccccaactg cccgtcgaac aagcgcccct acccgcccgg ccagcacggc cgcggccgca
      481 agcgcatctc cgagtatcag acccgtctgg tcgagaagca gaagctccgc gcgatctacg
      541 gcgtgagcga gagccagatg cgtcgctact acgacgaggc cacgcgcagc aagggcgtca
      601 ccggtgagga gatgatcgga cggctcgaga cccgcctgga caccgtggtg ctccggctcg
      661 ggttcgcgct gaccacccgt caggctcgcc agctcgtctc ccacgggcac gtcaccctgg
      721 acggcaagcg cctcaacatc ccgagcgcga aggtcaagcc gggccagacc gtggagatcg
      781 ccgccaaggc gaagaacttc gtggcggttc gtgaggccct tgagatcacg gccgacccgc
      841 cgccgtacct ctatcgcaac aaggacgagg tctcgggcac attgtcccgg gcgcccgagc
      901 gcaccgaggt gccgttgccg gtcgaggtcg aagagcgcct gatcgtcgag tactactcgt
      961 aacgatcgcc gggccgagcc cggttcgcag gagctcagga gctccctcgc ctatcctccc
     1021 tccagacgac cgaggaggac ggatgcagct gggtatggtc ggactgggac ggatgggcgc
     1081 caacctgtcc cgccgcttga tgcgtgccgg acatgaggtg gtcgcctacg acgtgagccc
     1141 cgaggcggtc gaaggcctcg ccgacgaagg cgccgtcggg gcatcctcgc tcgaagactt
     1201 cgtcgccaag ctcaccgccc cccgggcggc atggatcatg gtgccggcgg cgttcgcggg
     1261 ttccacggcc gagcagctcg ccgcgttgat ggagaccgac gacgtcatca tcgacggcgg
     1321 caactcctac taccgcgacg acatggcgcg atcggcagcc ttcgcgaagc gcggcatcca
     1381 ctacctggac gtcggcacga gtggcggcgt gttcgggttg gagcggggtt tctgcatgat
     1441 gatcggcggc gaccccgcgc ccgtgcaacg cctcgatccg atcttctccg ccctggctcc
     1501 cggcatcgag accgcggagc gcacgcccgg ccgggcggag tccggacgtg gtggcaccgc
     1561 cgagcacggt tacctgcact gtgggccgtc cggtgccggc cacttcgtga agatggtgca
     1621 caacggcatc gagtacggcc tgatggcggc tttcgccgaa ggcctcaacg tcttgcgcca
     1681 cgcgaacgtc ggtgccgccg atcgcgagga cgacgccgag accgctccgt tgcgcgaacc
     1741 cgagttctac cggtacgacc tcgacatcgc cgacgtcgcc gaggtgtggc gccgaggcag
     1801 cgtcgtcagc tcgtggctgc tcgatctcac ggcgtcggcg ctggtgaacg atccgaccct
     1861 ggagggcttc accggccggg tgagcgattc gggcgagggc cggtggacgc tgcaagccgc
     1921 gatcgatgaa ggcgtgccgg cccaggtgct gtcggcggcc gtgttcgacc gattcgcctc
     1981 gcaaggatcg gcggagttcg ccaacaaggt gctctcggcc atgcgcaagg agttcggcgg
     2041 gcacgacgag aaggcttcga cgtgagccga acggcggtcg acggttcgcc cgcgtggcag
     2101 gcgctcgccg accatgccgt cgcgaaccgg tcgacgacgt tgcgggacct gttcgccgcc
     2161 gagggcgacc gcgggaaccg gctcacggcc gaagccggtg atctctacct cgactactcg
     2221 aagcacctgg tcaccgacga aacgctggcg ctgctgttgc aactcgccca ggacgcgggc
     2281 gtcgccgggc gccgcgacgc gatgttcgcc ggcgagcaca tcaatgtcac cgaggatcgg
     2341 gcggtgctcc acgtggcgct ccggatgcct cgggatgcca cgctcgtggt cgacggcgtc
     2401 gatgtggtgg ccgaggtgca cgaggtcttg gggcggatgg gtgcgttcgc cgatcgggtg
     2461 cgcagcgggg agtggctcgg cgccacgggc gagcgcatca ccaccgtggt caacatcggg
     2521 atcggcggct ccgacctcgg cccgtccatg gcctacgaag cgttgctgga cgtctcgcaa
     2581 cgcgatatcg ggtgccggtt cgtgtccaac gtggacggcg ccgacatcgc cgaggccacc
     2641 cgcgacctcg atcccgcccg cacgctcttc gtcatctcct ccaagacgtt cacgacgctg
     2701 gagaccctga cgaacgccca cagcgcccgc gagtggctgg tcgcctcgct cggcgaggac
     2761 gcggtcgcga accatttcgt ggcggtgtcc acgaacgccg agggcgtgac gacgttcggc
     2821 atcgaccccg agaacatgtt cgggttctgg gactgggttg gcggacggta ttcgatggac
     2881 tcggcgatcg gcctgtcgct gatgatcgcg atcggtcccg aggcgttcgc cgacatgctg
     2941 gccgggttcc acacgatcga cgaacatttc cgcaccgcgc cgttcgaacg caatctgccg
     3001 gtgctgcaag gcgtgctggg catctggtac cgcaacttcc tgggcgccca gaccaaggcc
     3061 atcctgccct acagccacca cctttcgaag ctgccggctt acctccagca gctcgacatg
     3121 gagtcgaacg gcaagtcggt cacgctggac ggggagccgg tcggctacga caccggtccg
     3181 atcgtgtggg gcacaccggg cacgaacggc cagcacgcgt actaccaact gatccaccag
     3241 ggcacgacgc tgatccccgc cgacttcatc gggttcgccc acgccaccga tccactcggc
     3301 gaccaccacg acctgctgat ggcgaacgtg ttcgcgcaga ccgaagcgct ggcgttcggc
     3361 aagacgcgcg aggaggtcga ggccgagggc gtgccggccc atcaggtccc tcatcggacc
     3421 ttccacggga accatccaac gtcgacgatc ctggcgccct cattgacgcc gaacaccctc
     3481 gggcaactga tcgccctcta cgagcacacg gtgttcgtgc agggcacgat ctgggacatc
     3541 gactcgttcg atcagtgggg cgtcgagctg ggcaaggtgc tggcgggccg gatcgcgacc
     3601 gagctcgccg ccccgtcgga tccggacgcc ggcgcgcacg attcgtcgac catggccttg
     3661 atcagccgct accgtcgtct gcgagacgac gatccggaag ggacctcacg atgaccctgc
     3721 agccgccgat cccgcaagcc atcgtcgtgt tcggcgccag cggcgacctc gcgaagaaga
     3781 agatcctgcc ggcgatctac aacttggcgg tcgacggcct gctcccgccg gcatatgagg
     3841 tgatcgggtt cgcctcctcg gacatggacg acgagcgctt ccgtcagcac gctcgcgatg
     3901 cgatcgagga gcattcacgc cggccgatcg acgaggcggt gtggaacgcg ctcgcaccca
     3961 agttccggta catcatgggg tcgttcgagg accccgaggc gatgaagcga ctgggtgagg
     4021 tcctggacga ggccgaccgc gtccacgggt gcgacgggtg ccggctgttc tacatggcgg
     4081 tgcctcccac cctgttcccc gtgatcgcgc gatcactgga cgccgccggc ctcaacagcg
     4141 agctcggtcg cttggtggtc gagaagccgt acggcgactc gctcgagtcg gcgaaagcac
     4201 tcacgaacgc cctctacgag ggcttcaccg aggagcagat cttccggatc gaccactacc
     4261 tgggcaagga gaccgtccag aacctggtgg tattccggtt cgcgaactcg atctgggaac
     4321 gcgtctggaa ccgcgacgcg gtggactcgg tgcagctcac ggtggccgaa tcgatcggcg
     4381 tcgagggtcg cgccggcta
//
LOCUS       DATDBZ010000049         3662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_11164,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000049 DATDBZ010000000
VERSION     DATDBZ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11164"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..151)
                     /locus_tag="VIX62_01280"
     tRNA            complement(79..151)
                     /locus_tag="VIX62_01280"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Ala,seq:ggc)
     gene            complement(165..581)
                     /gene="rsfS"
                     /locus_tag="VIX62_01285"
     CDS             complement(165..581)
                     /gene="rsfS"
                     /locus_tag="VIX62_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY5858901.1"
                     /translation="MTETDRETREGDSPSLPPSRDVAIAAARAASDKQAGDIVVLDVH
                     DIIVITDFFVICSAGTQRQIRTVIDAVEASLRGLDVKPLRREGEPEAGWWLLDYVDVV
                     VHVFGDEERAYYDLERLWSDAPRVEWEQTDAASSAG"
     gene            complement(568..1665)
                     /locus_tag="VIX62_01290"
     CDS             complement(568..1665)
                     /locus_tag="VIX62_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY5858902.1"
                     /translation="MSRQRPEGAPVRASRAGRQAERRRRNVLAGIAIVVVLALVAFFV
                     VRRGGDDTPAAGSNDGASTAPGAEETMAFQVSGTTAPMMAVVGKGSEPVAMPYPQQLT
                     VVMPGRGETTAPDVAGLPATSMRIALSNMSGIWLAHYAVVTVNGLADAIDREGGLPVT
                     LTAAYPTKAGPIGPGSVTLTGAQTKAFLTGTTDDAGTRWELVLKALLANPPTFEPAEI
                     DETDDAAGAAEVFSAAKGAEITDVPTDLVTESIEVPAYPELDALMTGFFDTSTPTPAI
                     VQNGSGAPGVGEGVATQIIPAGFRITLSQNAQTFDVETTDVFANGVEFEEAAKRAREA
                     LGVGRVRVSQVASGIGDITIVVGKDFTVDGD"
     gene            complement(1662..2267)
                     /gene="nadD"
                     /locus_tag="VIX62_01295"
     CDS             complement(1662..2267)
                     /gene="nadD"
                     /locus_tag="VIX62_01295"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY5858903.1"
                     /translation="MGAVGTRLGIMGGTFDPIHYGHLVTAEEALHQFGLDGVVFVPTG
                     QPWMKEHEVVSGPEDRYLMTVIATASNPLFRVSRMEVDRDGPTYTVDTLRGMKAELGE
                     DTDLFFVTGADAILEIFQWKHREELFDLAHFIAATRPGYDIEAFGAHRPSSVPDITVM
                     NIPALAISSTDVRTRVHAGSPIRYLVPEGVKSYIEKAGLYR"
     gene            complement(2269..2718)
                     /locus_tag="VIX62_01300"
     CDS             complement(2269..2718)
                     /locus_tag="VIX62_01300"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5858904.1"
                     /translation="MDLEGLLAADESGWKELVELFAQIPAARFDEPTVTPEGWSPKDV
                     MFHVAAWMADAGLQLERMRAGTFNPSEETPASIQERNGQLFEASRAMEPSDVRIEFFS
                     AHQRMCEELGEMPELTPEAVEWFEESGPLHYAQHIKDLRGWLGDAAS"
     gene            complement(2719..>3662)
                     /gene="cgtA"
                     /locus_tag="VIX62_01305"
     CDS             complement(2719..>3662)
                     /gene="cgtA"
                     /locus_tag="VIX62_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEY5858905.1"
                     /translation="TRAVVAAGGRGGRGNTAFAGPRNRVPRTAELGEDGEDRTVTVEL
                     RTVADVGLVGLPNAGKSTLLARLTAAKPKIANYPFTTLTPNLGVAGEDTDRFVVADIP
                     GLVEGASEGKGLGHRFLRHIVRCRALVLVVDLAAEDPGTDLATLVAELEAYDSELARR
                     PSIVVGTKTDLVDDAASAIAALGPEAIPVSAMTGDGIEDLLDRVGLLAEEGEAAEPER
                     SPYVVLRPGKPRFTVSREDSGRWRVQGRSVEQWVLATDLDDDQDVERLQQRMAKEGVE
                     RKLVSLGARHGDEVAILDRVFEFLPDVDPEAVEEGDA"
BASE COUNT          548 a         1330 c         1199 g          585 t
ORIGIN      
        1 ccaaggcggg cacgcgaaga aggtgcatcc ggccactcga acctggccac tcgaaccttg
       61 ggtaggggac tggtggagtg gacccaaggg gagtcgaacc cctgacctct tggctgccag
      121 ccaagcgctc taccagctga gctatgggcc cttgcgcgac gattctaccc agccgaggag
      181 gctgcgtccg tctgctccca ctcgacccgg ggagcatcgc tccagagccg ttccaggtcg
      241 taataggcgc gttcttcgtc gccgaacacg tgcacgacga cgtcgacgta gtcgagcagc
      301 caccagccgg cctcgggctc accctcgcgc cggagcggct tgacgtccaa gcctcgcagt
      361 gacgcttcca ccgcgtcgat cacggtgcgg atctgccgct gggtgcccgc cgagcagatc
      421 acgaagaagt cggtgatgac gatgatgtcg tggacatcga gcacgacgat gtcgcccgcc
      481 tgcttgtccg aagccgcgcg ggccgccgcg atcgcgacgt cgcggctggg cggcagcgaa
      541 gggctgtcgc cttcccgcgt ttcccgatca gtctccgtca accgtgaagt ccttccccac
      601 gacgatcgtg atatcgccga tacccgatgc tacctgggac acccgcactc ggcccacccc
      661 cagggcctcc cgggcgcgtt tggcggcctc ctcgaactcc accccgttgg cgaatacgtc
      721 cgtggtctcc acgtcgaagg tctgggcgtt ctgcgacaag gtgatccgga atcccgccgg
      781 gatgatctgg gtcgccacgc cctcccccac gcctggcgcg ccgctgccgt tctgcacgat
      841 ggcgggcgtc ggcgtggagg tgtcgaagaa cccggtcatc aaggcgtcca actcgggata
      901 ggcgggcacc tcgatcgatt cggtcaccag gtcggtggga acgtcggtga tctccgcgcc
      961 cttcgcggca ctgaacacct ccgcggctcc ggccgcgtca tcggtctcgt cgatctccgc
     1021 cggctcgaac gtgggcgggt ttgcgagcaa cgccttcagc acgagctccc accgggtgcc
     1081 ggcgtcgtcg gtggtgcccg tgaggaacgc cttggtctgg gcgccggtca gggtcaccga
     1141 cccgggcccg atcggcccgg ccttcgtggg ataggccgcg gtgagcgtca cgggcaggcc
     1201 gccctcacgg tcgatcgcgt ccgcgagacc gttcacggtc accacggcgt agtgcgcgag
     1261 ccagatgccc gacatgttcg acagcgcgat gcgcatcgag gtcgcgggca gcccggcgac
     1321 atcgggggcg gtcgtttcgc cgcggcccgg catgaccacg gtgagctgct gcggatacgg
     1381 catggccacc ggttcgctgc ccttgccgac caccgccatc atcggcgccg tcgtccccga
     1441 cacctggaag gccatcgtct cctccgcccc aggggcggtg gacgcgccgt cgttcgaacc
     1501 cgccgccggc gtgtcgtctc ccccgcggcg cacgacgaag aaggcgacga gcgccaggac
     1561 gacgacgatc gcgatgccgg ccaggacgtt gcggcggcgc cgctcggcct gtcggcccgc
     1621 acgcgacgcc cgaacgggag ccccctcggg acgttgacgg ctcaccggta caggcccgcc
     1681 ttctcgatgt agctcttgac gccttcgggc accaggtagc ggatcgggct ccccgcgtgc
     1741 acgcgggtgc gcacgtccgt cgaggagatc gccagcgcgg ggatgttcat cacggtgatg
     1801 tccggcacgc tcgagggccg gtgcgcgccg aatgcctcga tgtcgtagcc gggacgagtg
     1861 gcggcgatga agtgcgccag atcgaacagc tcctcgcggt gcttccactg gaagatctcc
     1921 aggatcgcgt cggcgccggt cacgaagaac aggtcggtgt cctcccccag ttcggccttc
     1981 atgccccgca gggtatcgac cgtgtacgtc ggaccgtcgc ggtccacctc catccgcgag
     2041 acacggaaca aggggttgga ggccgtggcg atcaccgtca tcaggtagcg gtcctcggga
     2101 ccggaaacca cctcgtgttc cttcatccac ggttggccgg tcggcacgaa gacgacccca
     2161 tccaacccga actggtgcag cgcctcctcg gcggtgacga ggtgtccgta gtggatgggg
     2221 tcgaacgtgc cacccatgat ccccaggcgc gtccccacgg cgcccatcct acgaggcagc
     2281 gtcacccagc cacccgcgga gatccttgat gtgctgggcg tagtgcagcg gcccggattc
     2341 ttcgaaccac tcgaccgctt cgggcgtcag ttcgggcatc tcgcccagct cctcgcacat
     2401 ccgttgatgc gccgagaaga actcgatccg cacgtcgctc ggttccatcg ccctggaagc
     2461 ttcgaacaac tggccgttgc gctcctggat ggaggcgggg gtctcctcgg aggggttgaa
     2521 cgtgccggcc cgcatgcgct cgagttgcag gcccgcatcg gccatccacg ccgcgacgtg
     2581 gaacatcaca tccttgggcg accacccctc gggggtcacg gtgggctcgt cgaaccgcgc
     2641 ggcagggatc tgcgcgaaca gctcgaccag ttccttccac cccgactcgt cggccgccaa
     2701 caggccttcc agatccatct acgcgtcccc ctcctcgacc gcctccgggt cgacgtccgg
     2761 caggaactcg aacacccggt ccaagatggc gacctcgtcg ccgtgccgcg cgcccaacga
     2821 gaccagcttg cgttcgacgc cttccttggc catgcgttgc tgcagccgct cgacatcctg
     2881 gtcatcgtcg aggtcggttg ccaacaccca ctgttcgacg ctgcgaccct gcacgcgcca
     2941 ccggccgctg tcctcacggc tcacggtgaa ccggggcttg ccggggcgca gcacgacgta
     3001 gggcgagcgt tcgggttcgg cggcctcgcc ctcctcggcc agcaggccga cgcggtcgag
     3061 caggtcctcg atcccgtccc cggtcatcgc cgacaccggg atggcctccg gacccagcgc
     3121 cgcgatcgcc gaggcggcgt catcgaccag gtcggtcttc gtgcccacca cgatggacgg
     3181 gcgccgtgcc aactcggagt cgtaggcctc cagctcggca acgagggtcg ccaggtcagt
     3241 cccggggtcc tccgccgcca gatcgaccac caggacgagc gcccggcatc gcacgatgtg
     3301 ccgcaggaac cggtgcccca gcccctttcc ctcgctggct ccttccacca agccggggat
     3361 gtcggccacc acgaaccggt ccgtgtcctc gcccgccacg ccgaggttgg gcgtcagcgt
     3421 cgtgaaggga tagttggcga tcttgggttt ggcggcggtg aggcgagcca gcaacgtgga
     3481 cttgcccgcg ttcggtaacc ccaccaggcc aacgtctgcc accgtgcgga gctccaccgt
     3541 gaccgtgcga tcctccccgt cctcgccgag ctcggcggtg cggggcacgc ggttccgagg
     3601 tccggcgaac gccgtgttgc cccggccccc gcggccgcct gcggccacca cggcgcgcgt
     3661 gc
//
LOCUS       DATDBZ010000050         3672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2472496, whole genome shotgun sequence.
ACCESSION   DATDBZ010000050 DATDBZ010000000
VERSION     DATDBZ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472496"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="VIX62_01310"
     CDS             complement(<1..2067)
                     /locus_tag="VIX62_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5858906.1"
                     /translation="MSTQPTPAFEACPRCGHALPPDANFCPNCGAPVGLSPASERRVV
                     TVVFADLAGSTELAAHLDPERFREVLAAFHGVVTDEINALGGRAEGFIGDAVLGVFGV
                     PTVHDDDALRGVRAGLEIVDRASRLGARLGLTMPIEVRVGVNTGRVAVGTATDRTIVI
                     GAEVNIGARLQQAAHPGEVLVGAATRQLVGNGVRFGQVRPVEAKGLDGAMAAWPALDL
                     AGPATRRSLHLVNRRRELALLDDTFERVKVRERAHLVTLLGEPGIGKTRVVEEFLGGL
                     DDDVRVLAGRSSPFEEEVTFWPLAEMVYREIDAERDAPDEEIAERLRSLVDGWVEPED
                     ADDVVRRLALALGVDDDGSSEEGRYHAAEVRAGLLALLTGLASRGPVVLVFEDLHEAD
                     PLLLDLIEQLVKEARKVPLMVLCVARWEFLEGRSNWAGGIADAVTLWVEALTPGHAAE
                     LAMEAGDLAYEEAERVALHAGGNPYFVVEMVGMMRREERDLPPRGPAASARLLPPTVQ
                     AVIAARIDQLSAAARAMVQRASVFPRGRFDLEELSLIAEPRKDLLSEAEDEGLLVPDE
                     DRPGLWRFGSDILRDVAYDTLAKRERQRLHLRVANKLSEGETAGRYPRTIAFHLEQAA
                     RAALDLNPGDRQIAERAVEALAAAGDQSRRRIESRAAVDLYERALALAGPEEGWGPRE
                     AWIVSML"
     gene            complement(2091..2444)
                     /locus_tag="VIX62_01315"
     CDS             complement(2091..2444)
                     /locus_tag="VIX62_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5858907.1"
                     /translation="MIGPIDHVYYWTRDMDVSVAFYRDVVGLTLVRRDGNEWAEFEAG
                     PIRLALHGSGERPASGAGTVVFRVEDLDGTRWVLEQRGASFEDHLGEVEGFARFATFH
                     DPDGNPLQIIEYRSG"
     gene            complement(2448..3158)
                     /locus_tag="VIX62_01320"
     CDS             complement(2448..3158)
                     /locus_tag="VIX62_01320"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY5858908.1"
                     /translation="MAFPRKLLIPGEQLVLELRPHPVALAFSALITFIVMAVGIWLIS
                     AFDFDWIVYAAILIALIAYPLRHLVSWLTSYFVVTSDRVIQREGWISKRSIEIPLEQI
                     NDIRFEQGVFERVIGAGDIHIRSASNDGPTTFRDIRHPEEVQRTVYHQAELNNQRMYH
                     GGAATGAGLSPGAPVPQGAGVPPPAPVAAAPIRPDGSAPHAPSTTTELERLADLRARG
                     VLTEEEFQAQKAKILGQG"
     gene            complement(3200..>3672)
                     /locus_tag="VIX62_01325"
     CDS             complement(3200..>3672)
                     /locus_tag="VIX62_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858909.1"
                     /translation="LEVRCKWPNDLLVDGRKVGGILAESAGGAGLDQFEPDQTAGAVA
                     HVVIGSGINLVAPPEIEEAVGLGAGTDPTELLGGFLRRFREGYGSHGEHAASRSLDRW
                     RAVADTLGRRVRAHRLDGEPIEGVARDVDEHGGLVVETLAGTMTVRFGEVEYLG"
BASE COUNT          599 a         1318 c         1192 g          563 t
ORIGIN      
        1 gagcatcgac acgatccacg cctcgcgggg gccccacccc tcctcgggac cggcgagcgc
       61 cagcgctcgt tcgtagaggt cgacggcggc gcgcgactcg atccgtcggc gcgactggtc
      121 gccggcggcg gcgagcgcct cgacggcgcg ctcggcgatc tggcggtcgc ccgggttgag
      181 atcgagcgcc gcgcgcgccg cctgctccag gtggaacgcg atcgtgcgcg ggtagcgacc
      241 agcggtctcc ccctcggaca acttgttggc gacacgcaga tgaagtcgct gacgctcacg
      301 cttggcgagc gtgtcgtagg cgacgtcacg caggatgtcc gaaccgaatc gccacagtcc
      361 gggccggtct tcgtcgggca ccaacagtcc ttcatcctcg gcctcggaga gcagatcctt
      421 gcgaggctcg gcgatgagtg agagctcctc cagatcgaac cgaccacgcg ggaacaccga
      481 ggcgcgttgc accatggcac gggccgccgc gctcaactgg tcgatgcgtg ccgcgatcac
      541 ggcttgcacc gtcggtggca gcaatcgggc gctcgccgcg gggccgcggg gcggcagatc
      601 gcgttcctcc cgtcgcatca tgccgaccat ctccaccacg aagtaggggt tgcctccggc
      661 gtgcagggcg acgcgctcag cctcctcgta cgcgaggtcg ccggcctcca tcgcgagctc
      721 ggcggcgtgc ccgggcgtga gtgcttcgac ccacaacgtc acggcgtccg cgatgccgcc
      781 cgcccagttc gagcgccctt ccaagaactc ccaccgcgcc acacagagca ccatcagagg
      841 aaccttgcgg gcttccttca cgagttgctc gatcaggtcc aacaggaggg ggtcggcttc
      901 gtgcaggtct tcgaacacca atacgaccgg gccgcgggag gccaggcccg tgaggagcgc
      961 gagcagaccg gctcggacct cggcggcgtg gtagcggccc tcctcggatg aaccgtcgtc
     1021 gtcgacaccc agcgccaggg cgagacgcct gacgacatcg tcggcgtcct cgggctcgac
     1081 ccacccgtcg accagtgacc gcaagcgttc ggcgatctcc tcgtccgggg catctcgctc
     1141 ggcatcgatc tcgcggtaga ccatctccgc gagcggccag aacgtcacct cttcttcgaa
     1201 cgggctggaa cgaccggcga ggaccctgac atcgtcatcg agccctccga ggaactcctc
     1261 gacgacccgg gtcttgccga tgcccggttc gcccagcagg gtcacgaggt gggctcgttc
     1321 gcggaccttc acccgctcga acgtgtcatc caacaaggcc agctcacggc gccggttcac
     1381 caggtggagc gaacggcggg tggctgggcc ggcgagatcc aacgccggcc acgcggccat
     1441 cgccccgtcc agccctttgg cttcgaccgg gcgaacctgg ccgaatcgca caccgttgcc
     1501 gaccagttgc cgggtcgctg cgcccaccag gacctcgcct ggatgggctg cctgctgtag
     1561 ccgagccccg atgttgacct ccgcgccgat cacgatcgtg cggtcggtcg ctgtgcccac
     1621 cgcgacccgg ccggtattga cgcccacccg aacctcgatc ggcatcgtga gaccgagtct
     1681 ggcacccaac cgagatgcgc gatccacgat ctccagacct gctcgcaccc ctcgcagggc
     1741 gtcgtcgtcg tgcacggtcg gcaccccgaa cacgccgagg accgcgtccc cgatgaaccc
     1801 ttcggcgcgc cctcccaggg cgttgatctc gtcggtgacc acgccatgga acgcagccag
     1861 cacctctcgg aaccgctcgg gatccagatg ggccgccaac tccgtggaac ccgccaggtc
     1921 ggcgaacacc acggtcacca cccgtcgctc ggacgcaggc gacaacccca ccggcgcgcc
     1981 acagttcgga cagaaattgg cgtctggcgg gagtgcatgt ccgcagcggg gacacgcctc
     2041 gaacgccggg gttggctggg tgctcacgcg ccccagcata ggtggcagac tcagccgctt
     2101 cgatactcga tgatctgcag cgggttgccg tccgggtcgt ggaaggtggc gaacctcgcg
     2161 aacccttcga cctcgcccag atggtcctcg aaggaggcac cacgttgttc gagcacccat
     2221 cgggtaccgt ccaggtcttc gacgcggaac accacggtgc ctgcacctga tgcggggcgc
     2281 tcaccggacc cgtggagcgc caaccggatg ggcccggcct cgaactcggc ccactcgttc
     2341 ccgtcgcgtc ggacgagcgt gagccccacc acgtcccgat agaacgccac cgacacgtcc
     2401 atgtcccgcg tccagtagta gacgtggtcg atggggccga tcatgggtca gccctggccc
     2461 aagatcttcg ccttctgggc ctggaactct tcttcggtga gcacacctcg agcacgcagg
     2521 tccgcgagcc gctcgagttc cgtggtggtc gagggcgcgt gcggcgccga cccgtcgggc
     2581 cggatcggag cagctgccac cggtgcgggc ggcggtaccc ctgcaccctg gggcacgggg
     2641 gcacccgggg acagccccgc gcccgtcgcc gctccaccgt ggtacatgcg ttggttgttc
     2701 aactccgcct ggtggtagac cgtcctctgc acctcttcgg ggtgacggat gtcacggaac
     2761 gtggtgggtc cgtcgttgct cgccgagcgg atgtggatgt cccctgcacc gatgacgcgc
     2821 tcgaacacgc cctgctcgaa gcggatgtcg ttgatctgct ccagcgggat ctcgatcgag
     2881 cgcttcgaga tccagccttc tcgttggatc acccgatcac tcgtgaccac gaaataggag
     2941 gtgagccagc tgacgaggtg gcgcagcggg taggcgatga gcgcgatcag gatcgccgca
     3001 tagacgatcc agtcgaagtc gaacgcgctg atcagccaga tgcccaccgc catcacgatg
     3061 aacgtgatca gcgcggagaa cgccagggcc accggatggg gacgaagttc caggaccagt
     3121 tgctcgccgg ggatcaagag tttccgaggg aatgccatgg ccgaacggta ccgcccacgt
     3181 cggtggtggg caaccgacct cagcccagat actcgacctc gccgaagcgc actgtcatgg
     3241 ttccggcgag ggtctcgacg accaagccac cgtgctcgtc cacgtctcgc gcgacccctt
     3301 cgatcggttc gccgtccagc cggtgtgctc ggacgcgccg acccagcgtg tcggccacgg
     3361 cgcgccaccg atccaacgac cgcgatgccg cgtgctcgcc gtgggatccg tagccctccc
     3421 ggaaccgccg caggaacccg ccgaggagtt cggtggggtc ggtgccagcg ccgaggccaa
     3481 cggcctcttc gatctccggc ggtgccacta ggttgatccc ggaaccgatc acgacatgcg
     3541 ccacagcacc ggcggtctga tcgggctcga actggtcgag ccctgccccg cccgcggact
     3601 ccgcaaggat gcctcccacc ttgcgaccgt cgaccagcag gtcgttgggc cacttgcagc
     3661 gaacctcgag cc
//
LOCUS       DATDBZ010000051         4090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_836529, whole genome shotgun sequence.
ACCESSION   DATDBZ010000051 DATDBZ010000000
VERSION     DATDBZ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836529"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VIX62_01330"
     CDS             <1..1026
                     /locus_tag="VIX62_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013332308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY5858910.1"
                     /translation="GEEVVVRFDASTGAWMFVGVHSTVLGPAMGGTRLKAYASPDDAL
                     VDVLRLSAAMTFKQAAADLPYGGGKAVIAVPAVPTPQERGELLRRYGRLVDSLHGTYV
                     TAADMNTNQTDMDVIGEVTSHVLGRSREAGGSGDPAPGTAVGVFHGIVASVRHAFGSG
                     DLADVRVLVQGVGSVGGRLIGLLRDAGARVLVSDVDEVRAGEAAASVDGPVVAPGDVI
                     GTECDVYAPCATGAVLDAESIPALRCPVVAGAANNQLATAEDAGRLRAAGVLYAPDYV
                     INAGGVIHLAGYERFGWDGAAVAARLAGIGQTLTDVFESADREGITTAEAADRLAQER
                     LATGPRD"
     gene            complement(1046..2716)
                     /locus_tag="VIX62_01335"
     CDS             complement(1046..2716)
                     /locus_tag="VIX62_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY5858911.1"
                     /translation="MSGAVADPLGPIDEFAEQVMVPMADGIRLATDVYLSRGGRSADR
                     SPCILVRLPYDKSAPFAFMPRLAAWFTERGYAFVAQDVRGRARSEGETFAFVHEVSDG
                     SATLDWIVDQPWSNGVVGMFGDSYYGWTQWAAVASGHAALRAIVPRVTSVEVGTDWRE
                     TGGVFNAYQMIEWAAGTWSGTENFECDLGWSVRPLADVQADAHGGRRSRSLDHWISVA
                     GDDPWWWEALYAGRGDPRARLRVPALHTGGWWDVFRRGQIRDFHAVHGRVPDQHLVME
                     ATDHFDDWLRDRDAPPFVDFIDDAAEMERWLPAYAGPATAFFDRYLRGKDGDGRIPEV
                     RWFCAHDGWREAATWPPPQARLMRLSLTGAASAAGNGGGLAPRGDRDASWSRWTHDPA
                     NPVPDGIEDAWRSLVSLPDERDVEARPDVLTFTGDPVDADLDLAGPVIASLRVSSTAP
                     STHAVAKLVDVAPDGFAQRIVEGIVAVPRTGSRDEARDAVVDLGSTGYRVREGHRLRL
                     EVATSCFPRFAVHPGTSEDPMRAIEGTPSDQGLLVGGTEGSAVSVTVI"
     gene            complement(2713..3111)
                     /locus_tag="VIX62_01340"
     CDS             complement(2713..3111)
                     /locus_tag="VIX62_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858912.1"
                     /translation="MTLLRRTLKVQAALWLLWSLALGLAPRLVLEDLFSQPILAEYAW
                     VRASAVMGAVLALLMVLVAQRIEELWWWSWAFAVLEVGTATVFAINALFGVPEGAAAW
                     PWWGLAAINASFGALLLAGMGLAGQEKPFV"
     gene            3266..>4090
                     /locus_tag="VIX62_01345"
     CDS             3266..>4090
                     /locus_tag="VIX62_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5858913.1"
                     /translation="MQQGPRVAVIGLDCGTPQLLFHDLQAEVPNIAKLMGEGAYGNLA
                     SITPPITVPAWACAMTGKVPGQLGIYGFRNRKDTTYDGLAIAHSGSIKEPAVWDKLGE
                     QGMRSVLIGVPPSFPPPKEFPGWRVGCFLTPPSAERWAFPQPLEAEIEEELEGKTYIF
                     DIPNFRQAGMDVTLDQVFKMTERRFQVGRRLIQNKPWDFFMLCDIALDRLHHVFWQYF
                     DPRHPLYQPGNPYEATFRDYYRFLDQQVGTLLELIPDDAITMVMSDHGARPMMGGLC"
BASE COUNT          646 a         1456 c         1408 g          580 t
ORIGIN      
        1 ggggaagagg tcgtcgttcg gttcgatgca tcgaccggcg cgtggatgtt cgtgggcgtg
       61 cactcgaccg tgctcgggcc ggcgatgggt ggaactcggc tgaaggctta tgcgtcgccg
      121 gacgatgcgc tggtggacgt gctcaggctc tcggcggcga tgaccttcaa gcaggcggcg
      181 gcggatctgc cttacggcgg gggcaaggcg gtgatcgccg ttccggcggt gccgacgccg
      241 caggagcgag gggaactgct gcgacggtac gggcggctgg tggactcgct gcatgggacc
      301 tacgtcacgg ccgccgacat gaacacgaac cagaccgaca tggatgtgat cggcgaggtc
      361 acatcccacg tgctgggacg ttcgcgtgag gcgggaggct cgggcgatcc cgcgcccggc
      421 accgcggtgg gggtcttcca cgggatcgtg gcgagcgtgc ggcatgcctt cggctcgggc
      481 gacctggcgg acgtgcgggt gttggtgcaa ggggtcggat cggtgggggg gcggctgatc
      541 ggcctgctcc gcgatgctgg ggcccgggtg ctggtctcgg acgtcgacga ggtccgcgcc
      601 ggcgaagctg cggcttccgt cgacgggccg gtggtggcgc cgggcgatgt gatcggcacc
      661 gagtgtgacg tctacgcgcc gtgcgcgacc ggcgccgtgc tggacgccga gtcgatcccg
      721 gcgctgcggt gcccggtggt ggcgggggcg gccaacaacc aattggccac ggccgaggac
      781 gcgggccggt tgcgagcggc gggcgtcctg tacgcgcccg actacgtcat caacgcgggt
      841 ggggtgatcc acctggctgg atacgagcgg ttcgggtggg acggggcggc ggtggcggcc
      901 cggctggcgg ggatcggaca gacgttgacc gacgtgttcg aaagcgccga ccgcgagggg
      961 atcacgaccg ccgaggccgc cgaccgactg gcgcaggagc ggttggcgac cggaccccgg
     1021 gactgaccgg ggggtctggg acgcgctaga tcacggtcac cgacacggcg gacccctcgg
     1081 tgccgcccac cagcaaccct tggtcgctcg gtgtgccttc gatcgcccgc atcgggtctt
     1141 cgctggtgcc gggatggacc gcgaatcgcg ggaagcagct cgtcgccacc tcgagccgga
     1201 gccggtgccc ctcgcgcacc cggtaccccg tcgagccgag gtccaccacc gcgtccctcg
     1261 cttcgtcgcg cgagcccgtc cgcggcaccg ccacgatccc ctcgacgatc cgctgcgcga
     1321 acccgtcggg cgcgacatcg acgagcttgg ccacggcgtg ggtcgagggc gccgtcgacg
     1381 acacccgcag cgaggcgatc accggccccg cgagatcgag gtcggcatcc accggatcgc
     1441 cggtgaacgt cagcacatcg ggacgggcct caacgtcgcg ctcgtcgggc agcgacacca
     1501 acgaccgcca ggcatcctcg atgccgtcgg gcaccgggtt cgccggatcg tgggtccagc
     1561 gcgaccacga ggcatcacgg tcaccccgcg gcgcgagccc gcctccgtta ccggcggccg
     1621 acgccgcccc cgtcaacgac aagcgcatga gccgcgcctg gggcggtggc catgtcgccg
     1681 cctcacgcca gccgtcgtgc gcacagaacc accggacctc cgggatccgg ccatcgccgt
     1741 ccttcccgcg caggtaccga tcgaagaacg ccgtcgccgg cccggcgtag gccggcagcc
     1801 accgttccat ctcggcggca tcgtcgatga agtccacgaa cggcggcgca tcgcgatcgc
     1861 ggagccagtc atcgaagtga tcggtggcct ccatgacgag gtgctggtcc ggcacgcggc
     1921 cgtgcacggc atggaaatca cggatctgcc cccgccggaa cacatcccac cagccgccgg
     1981 tgtggagcgc cggcacgcgc aaccgtgctc gcggatcgcc tcggccggcg tacagcgcct
     2041 cccaccacca cggatcgtcg ccggccaccg agatccagtg atcgagcgac cgcgaccgac
     2101 gcccaccgtg cgcatcggcc tgcacgtcgg cgagcggccg cacgctccag ccgaggtcac
     2161 actcgaagtt ctccgtcccc gaccaggttc ccgcggccca ttcgatcatc tgataggcgt
     2221 tgaacacccc gccggtctcg cgccagtccg tgccgacctc caccgacgtc acccggggga
     2281 cgatcgccct gagcgcagcg tgcccgctcg ccaccgccgc ccactgcgtc cagccgtagt
     2341 aggagtcccc gaacatcccc accacgccgt tcgaccacgg ctggtccacg atccagtcca
     2401 gggtcgcgga accgtcgctc acctcgtgca cgaacgcgaa cgtctcgccc tcgctgcgag
     2461 cccgcccacg gacgtcctgg gcgacgaagg cgtagccacg ttccgtgaac cacgcggcga
     2521 gccgcggcat gaacgcgaag ggtgcgctct tgtcgtaggg gagccgcacc aggatgcagg
     2581 gcgatcgatc cgcgctgcgt ccgccgcgcg aaaggtagac atcggtggcg agccggatgc
     2641 cgtcggccat cggcaccatg acctgctcgg cgaactcgtc gatgggcccg aggggatccg
     2701 cgacggcgcc gctcatacga acggcttctc ctggcctgcc aggcccatgc ccgccagcag
     2761 caacgcgccg aacgaggcgt tgatcgccgc cagcccccac catggccagg ccgccgcccc
     2821 ttcgggcacc ccgaacagcg cgttgatcgc gaacaccgtc gccgtgccca cctcgagcac
     2881 cgcgaacgcc cacgaccacc accagagctc ctcgatccgc tgggcgacca ggaccatcag
     2941 cagcgccagc acggcgccca tcacggccga ggcccggacc caggcgtatt cggccaggat
     3001 cggttgggag aacaggtctt ccagcaccag acgaggcgcc agaccgagcg cgaggctcca
     3061 caggagccag agggccgctt gcaccttcaa ggttcgccgg agcagggtca tggccgccta
     3121 tcttcgcagg ttggaggccc tgcgaagggt tgggagccct ccgggcggtt ggtaggatac
     3181 gcggaccgcg gaagctcccc ctggcgcccc agcgcgccct tcgtttccgc ctcttgaggg
     3241 ttcccgactc cggaaggatc gacgcatgca acaaggaccc agggtggccg tgatcgggct
     3301 cgactgcggt acgccgcagc tcctgttcca cgacctccag gccgaggtgc cgaacatcgc
     3361 caagctcatg ggagaaggcg cctacggcaa cctcgcttcg atcacgccgc ccatcacggt
     3421 gcctgcgtgg gcatgcgcga tgaccggcaa ggttcccgga cagctcggga tctacgggtt
     3481 ccgcaaccgc aaggacacca cctacgacgg gctcgcgatc gcccactcgg gttcgatcaa
     3541 ggagcccgcg gtgtgggaca agctcggtga gcagggtatg cggtcggtcc tgatcggcgt
     3601 gccgccgtcg ttcccaccgc ccaaggagtt ccccggctgg cgcgtcggct gcttcctgac
     3661 gccgccgtcg gccgagcgct gggccttccc gcagccgctc gaagccgaga tcgaagagga
     3721 actcgaaggc aagacctaca tcttcgacat cccgaacttc cgccaggcgg gcatggacgt
     3781 cacgctcgac caggtgttca agatgaccga gcgccggttc caggtgggac gtcgcctcat
     3841 ccagaacaag ccctgggact tcttcatgtt gtgcgacatc gcgctcgacc gtctgcacca
     3901 cgtgttctgg cagtatttcg acccacggca cccgctctat cagcccggga acccgtacga
     3961 ggccaccttc cgcgactact accgcttcct ggaccagcag gtgggcacgc tgctcgaact
     4021 gatccccgac gacgcgatca cgatggtgat gagcgatcac ggcgctcgtc cgatgatggg
     4081 gggcctgtgc
//
LOCUS       DATDBZ010000052         2778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_222816, whole genome shotgun sequence.
ACCESSION   DATDBZ010000052 DATDBZ010000000
VERSION     DATDBZ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222816"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VIX62_01350"
     CDS             <1..788
                     /locus_tag="VIX62_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5858914.1"
                     /translation="TNTPAENVALALERGLSGIAVTDHDTTEGLDEAFAAAEGTGLEI
                     VPGIEFSAEYQGASLHVLSYWVDPANAPLREELQRLTDTRFRRGEMMVEKLQELGYDI
                     SFERVRELADDGVIARPHVAKAMVEAGIVPTEKEAFDRFISDDGIAYVPKHALDPIDS
                     LDLIAGAGGICVLAHPGMWKGAGSVPDELIEQMAEGGMVGLEVDHPDHDETQRAYYHE
                     VARRLELIPTGASDCHGARYDYRMGCDTTDAERFAELQRRAGR"
     gene            803..1597
                     /locus_tag="VIX62_01355"
     CDS             803..1597
                     /locus_tag="VIX62_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017606232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY5858915.1"
                     /translation="MSPIERITGPADPRLRDFTELRDVQVRSRREPIEGLFIAEGEAT
                     IRRALDAGYVPRAVLTTDRWLTSMGDLLGSADAPVLVVDEQLLEATTGYPVHRGALAS
                     MPRRPLPSLADVVEGATRIVVLEDLVDHTNVGAVFRSAAALGMDAVVLTDRCADPLYR
                     RAIRTSMGAVFTVPWTRVDGPEALSEAGFELVALTPAADAVDLRSYDGAPKVALALGS
                     EGDGLSARWLEAADVRVFIPMAHGVDSLNVAAAAAVACYALGASGR"
     gene            1608..2351
                     /locus_tag="VIX62_01360"
     CDS             1608..2351
                     /locus_tag="VIX62_01360"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5858916.1"
                     /translation="MPPDPAADARHEAQPRAQPEFQPTILMVFFDIGGVMYDDTVYAR
                     SWMKALRESGAAFTDEEFEREYVAARAAQSGSFRRRLMRTFLGEDAELAPVEARAAKY
                     WAYPPTAIYSDVIPCLDALIDRYRLGIIANQPSSVRAAMDRDGLSPYFEVWGVSDDLG
                     LQKPDPKLFSQVLYTAGVAPTRAVMLGDRLDYDVRPAKTAGMRTVWVLRGEAPDDPTP
                     AQLAEPDASVRTLAEIPAMLESWEAASAS"
     gene            2327..>2778
                     /locus_tag="VIX62_01365"
     CDS             2327..>2778
                     /locus_tag="VIX62_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5858917.1"
                     /translation="MGSRKRVLIVGGGFAGMEAALHLKPLAKAGYDVTLVSSDNFMQY
                     QPFLPEVASGTIDPRAAVVPLRPLLRHTSIAIGEVMAIDHEANSATVTIPDGTSVALG
                     YDILVVTAGSWSRVLPVPGLAERGIGFKTVQEAIYLRNHVLSRLDVAA"
BASE COUNT          417 a          947 c          986 g          428 t
ORIGIN      
        1 gcacgaacac cccggccgag aacgtcgccc tggcgttgga gcgcggcctg tcggggatcg
       61 ccgtcaccga ccacgacacc accgaaggac tcgatgaggc gttcgccgct gcggagggca
      121 ccggcctgga gatcgtgccg gggatcgagt tctccgccga gtaccagggg gcgagcctgc
      181 acgttctgtc gtactgggtc gaccccgcga acgcgccgct gcgtgaggag ctgcagcgcc
      241 tcacggacac ccggttccgg cgcggcgaga tgatggtgga gaagctgcag gagctgggct
      301 acgacatctc gttcgagcgc gtccgcgagc tggccgacga cggcgtgatc gcccggccgc
      361 atgtcgccaa ggccatggtg gaggccggca tcgtgcccac cgagaaggaa gcgttcgacc
      421 ggttcatcag tgacgacggc atcgcctatg tccccaagca cgcgctcgac ccgatcgatt
      481 cgctcgacct catcgcgggc gccggcggca tctgcgtgct ggcccatccc gggatgtgga
      541 agggcgcggg ttccgttccc gatgagctga tcgagcagat ggccgaaggt ggcatggtcg
      601 gtttggaggt ggatcacccg gatcacgacg aaacgcagcg tgcctactac cacgaggtgg
      661 cacggcggct cgaactgatc cccaccggcg ccagcgactg tcacggggcg cgctacgact
      721 accgcatggg gtgcgacacc accgacgccg aacggttcgc cgagctgcag cgtcgcgcgg
      781 ggcgctagcg cccggtgccg ccgtgagtcc catcgaacgc atcactggcc cggccgatcc
      841 gcgcctccgc gacttcaccg agctgcgaga cgtccaggtc cggtcccgcc gggaaccgat
      901 cgagggactg ttcatcgccg aaggggaggc gacgatccgt cgcgcgctcg acgccgggta
      961 cgtccctcgc gcggtcctca ccaccgaccg gtggctgacg tcgatgggcg atctgctggg
     1021 gagcgccgac gcgccggtcc tggtcgtgga cgaacagctg ctggaggcca cgaccggcta
     1081 tccggtgcat cggggcgcgc tcgcctcgat gcctcggaga ccgttgccat cgctggccga
     1141 cgtggtcgaa ggcgccacgc ggatcgtggt cctggaagat ctcgtcgacc acacgaacgt
     1201 cggcgccgtg ttccgcagcg cggcagcgct ggggatggac gccgtcgtcc tgaccgatcg
     1261 gtgcgccgac ccgctgtacc ggcgcgcgat ccgaacgtcg atgggtgccg tgttcaccgt
     1321 gccgtggacc cgggtggacg gccccgaggc cctgagcgag gccggcttcg aactcgtggc
     1381 gctgacgccg gcggccgacg ccgtggacct tcgttcctac gacggtgcgc cgaaggtcgc
     1441 gctggccctc ggatcggaag gcgatggcct gtcggcccga tggctcgaag cggcggacgt
     1501 ccgcgtgttc atcccgatgg cgcacggggt cgattcgctg aacgtggcgg cagcggcagc
     1561 ggtcgcgtgc tacgcgcttg gagcatccgg ccgctagcat gccggtcgtg cccccggacc
     1621 ccgccgccga tgcccgacat gaggcccagc cgagggccca gccggagttc cagccaacca
     1681 tcctcatggt gttcttcgac atcggcggcg tgatgtacga cgacaccgtg tacgcgcgat
     1741 cgtggatgaa ggcgctgcgc gaatcgggcg cagcgttcac cgacgaggaa ttcgagcgcg
     1801 agtacgtcgc ggcccgggcc gcccagtccg gatcgttccg ccgccgcctc atgcgaacct
     1861 tcctgggtga ggatgcggag ctggcgcccg tcgaggcgcg tgccgcgaag tattgggcct
     1921 acccgcccac ggcgatctac tccgacgtga tcccctgcct ggatgccctc atcgaccgct
     1981 accggctggg gatcatcgcg aaccagccga gctcggtgcg cgccgcgatg gaccgcgacg
     2041 gtctcagtcc ctatttcgag gtgtggggcg tgagcgacga cctgggattg cagaagcccg
     2101 acccgaagct gttctcgcag gtgctctaca cggccggtgt ggcaccgacc cgcgcggtga
     2161 tgctggggga ccgcctcgac tacgacgtgc gtccggccaa gaccgccggg atgcggaccg
     2221 tctgggtgct gcgtggcgag gcaccggacg atcccacgcc cgcccaactg gccgagcccg
     2281 atgcgtccgt acggacgctc gccgagatcc ccgccatgct ggagtcatgg gaagccgcaa
     2341 gcgcgtcctg atcgtcggcg gtggcttcgc cggcatggag gctgccctcc acctgaagcc
     2401 gctggcgaag gccggctatg acgtcaccct ggtgagctcg gacaacttca tgcagtacca
     2461 gccgttcctg ccggaagtgg ccagcggcac gatcgacccc cgcgccgccg tggtgccgtt
     2521 gcggcctctg ttgcggcaca cctcgatcgc gatcggtgag gtcatggcga tcgatcacga
     2581 ggcgaactcg gcgacggtga cgatccccga cgggacttct gtcgcgctcg gctacgacat
     2641 cctggtggtg acggcggggt cgtggtcgcg cgtgctgccg gtgcccgggc tcgccgagcg
     2701 ggggatcggg ttcaagaccg tgcaggaggc gatctacctc cgcaaccacg tgttgtcccg
     2761 gttggacgtc gccgccga
//
LOCUS       DATDBZ010000053         2613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_222852, whole genome shotgun sequence.
ACCESSION   DATDBZ010000053 DATDBZ010000000
VERSION     DATDBZ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222852"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VIX62_01370"
     CDS             complement(<1..626)
                     /locus_tag="VIX62_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY5858918.1"
                     /translation="MPNPWDAGSARALEQMGFKALATTSAGFAWTSGHADNHVTLDQA
                     LDHLRDVAKAVTVPVNADFEGGYAVDPQQVGENVKLAAATGIAGLSIEDSSGDEAQPL
                     FEFDLAVERVRAARRAIDESGTGIVLTGRSEGFVCGRPDIDETIRRLRAYAEAGADCL
                     YAPRIGTEHIAAVVAAVSPKPVNLLINAPFITVAEAADLGVRRISVGGT"
     gene            873..1748
                     /locus_tag="VIX62_01375"
     CDS             873..1748
                     /locus_tag="VIX62_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858919.1"
                     /translation="MSARFARQEVSLMRLTVWRLALAGLASAALIMPASVPTAASPGP
                     RDPSSPIVAEQTTGGGASFMRVAITRHGNLWFASPKGVALTSFDEGYALCRRDEVGED
                     TAYAYDLPLEDVDPGDVGLGPVTVTQPTPGAFPVTIRRKTLDGTIRLTQIWALPDTTE
                     KDVTVTMTVRNVGPSTLEGLRLMRSADSHSDYADAPDSTFFRGATTADSVFQWWDADT
                     DGTANGLVMTARTVGVPHEAGLSSDVDLALYCGGFSEMEPSDLSSGGSPQIVYRLPSL
                     RPGAEVSVAFTYRRL"
     gene            complement(1775..2497)
                     /locus_tag="VIX62_01380"
     CDS             complement(1775..2497)
                     /locus_tag="VIX62_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY5858920.1"
                     /translation="MTAKGTLHLGTSGWSFDGWKHGVFYPEGLKNREMLHYYSTQLSS
                     VEINYTFRRFPTEKSLTTWREQAAEGFVFTLKANQRITHFKRLRDTDDDVRDFLDRAK
                     VLGDRLGCVLFQCPPNLHYDRELIERFVGYLPPGPPAFAMEFRHASWNEAKDLLRDQG
                     IAWCVAETDERDPKPEELSWEPVGYLRLRKTEYGDDELREWAGRIQPALDSGADIFCY
                     FKHEDDGFGPKMAKRLEGMVNP"
BASE COUNT          395 a          923 c          863 g          432 t
ORIGIN      
        1 gtgcctccca cgctgatccg ccgtacgccc aggtccgccg cctcggcgac cgtgatgaac
       61 ggggcgttga tcaagaggtt gaccggcttg ggcgacaccg cggcgacgac cgcggcgatg
      121 tgctcggtgc cgatgcgcgg agcgtagagg cagtcggcgc cggcttcggc gtaggcacgc
      181 agccgccgga tcgtctcgtc gatatcggga cgcccgcaca cgaaaccctc ggaccgcccg
      241 gtcagtacga tgccggtgcc actctcgtcg atggctcgtc gcgcggcccg gacacgctcg
      301 acggccaggt cgaactcgaa gagcggctgt gcctcgtcgc ccgaggaatc ctcgatcgag
      361 agaccggcga tgcctgtcgc ggcagccagc ttgacgttct cacccacctg ctgtggatcg
      421 acggcgtagc ctccctcgaa gtcggcgttc accggcaccg tcacggcctt cgcgacatca
      481 cggagatgat cgagcgcctg atccagcgtc acgtgattgt ccgcgtgacc cgatgtccat
      541 gcgaacccgg cgctcgtcgt cgcgagcgcc ttgaagccca tctgctccag cgctcgtgcg
      601 ctcccagcgt cccaggggtt cggcatcacg aagcagccgg cttcgtgcag tcggtggaac
      661 gcagcgaccc gctcgcgttg ctcgctcacg atgtacctca cctcccaggg aaggccgaag
      721 ctccgatggc tcgtgtgtcc agcgctcgct gaccctccca aggctctcac gggcacgctg
      781 atgctgaccc ggccctccag ccgatgtagg caggcccatc ggtggcgggc cgctcggcga
      841 ccgcgcggca tgcaggggaa cctacggtgt cggtgagcgc gagattcgcg cgtcaggagg
      901 tgagcctcat gcggctcacg gtgtggcgcc tcgccctcgc cggtctggcg agcgcagcgc
      961 tgatcatgcc cgcgtcggtt cccacggcgg catcgcccgg tccacgagat ccgagctcgc
     1021 cgatcgtggc cgagcagacc accggcggcg gggcgagctt catgcgcgtg gccatcaccc
     1081 ggcatgggaa cctgtggttc gcatcaccca agggcgtcgc gctgacctcg ttcgacgagg
     1141 gatacgcgct ctgccggcgc gacgaggtcg gcgaggacac ggcgtacgcc tacgacctgc
     1201 cgctcgagga cgtcgacccc ggcgacgtcg ggctcggtcc ggtcacggtc acgcagccca
     1261 cgccgggcgc gttcccggtc acgatcaggc gcaagactct cgatggcacc atccgcctta
     1321 cacagatatg ggcgctcccc gacacgaccg agaaggacgt caccgtcacc atgacggtgc
     1381 gcaacgtcgg tccatcgacg ctcgagggcc tgcgcctcat gcgcagcgcg gattcccact
     1441 cggactacgc tgatgctccc gattccacgt tcttccgagg cgccacgacc gccgactcgg
     1501 tcttccagtg gtgggacgcc gacaccgacg gcaccgcgaa cgggctcgtg atgacggctc
     1561 gcacggtcgg tgtgccgcac gaggcgggat tgtcgagcga cgtcgacctc gcgctgtact
     1621 gcgggggctt ctccgagatg gagccctccg atctcagcag cggcggctcg ccccagatcg
     1681 tgtaccgctt gccctcactt cgaccgggtg ctgaggtgtc ggtggcgttc acctaccgcc
     1741 ggctctgact tcccgaggag gtcggtcggt cacgctaggg gttcaccatg ccttccagtc
     1801 gcttggccat cttcgggccg aagccgtcgt cctcgtgctt gaagtagcag aagatgtcgg
     1861 cgcccgagtc cagcgccggc tggatgcgcc ccgcccactc gcgaagctcg tcgtcgccgt
     1921 actcggtctt gcgcaggcgc aggtagccca ccggctccca tgacagttcc tcgggcttcg
     1981 ggtcgcgctc gtcggtctcg gccacgcacc aggcgatgcc ctggtcgcgc agcaggtcct
     2041 tcgcttcgtt ccacgacgcg tggcggaact ccatcgcgaa cgccggaggt ccgggcggca
     2101 ggtagcccac gaaccgctcg atcagctcgc ggtcgtagtg caggttcggc gggcactgga
     2161 acaacacgca gccgagccgg tcgccgagca ccttcgcccg atcgaggaag tcgcgcacgt
     2221 cgtcgtcggt gtcgcgcagc cgcttgaagt gcgtgatgcg ctggttcgcc ttcagcgtga
     2281 acacgaaccc ctcggccgcc tgctcgcgcc aggtcgtgag cgacttctcg gtcgggaacc
     2341 gccggaacgt gtagttgatc tcgaccgagc tgagctgggt cgagtagtag tgcagcatct
     2401 cgcggttctt caggccctcg gggtagaaga ccccgtgctt ccagccgtcg aacgaccagc
     2461 ccgaggtgcc caggtgcaac gtgcccttcg ccgtcatggc atgcatgatg gcatgcgtgc
     2521 cgacgcccga ggaacgcttc acgatcgagg tgcccgacaa gggcacggtg agcgccgccc
     2581 tcacgcgccc cagcgacgcg ttcgcctcac tgg
//
LOCUS       DATDBZ010000054         2520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2884270, whole genome shotgun sequence.
ACCESSION   DATDBZ010000054 DATDBZ010000000
VERSION     DATDBZ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2884270"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..671)
                     /locus_tag="VIX62_01385"
     CDS             complement(111..671)
                     /locus_tag="VIX62_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858921.1"
                     /translation="MRAEISPAGRIAGFVTAGCIIVAGIAFIVTSWDELTCAKGPSCE
                     DWAGAAGLVSLVSLAAIGAGLRIAWTVWRRPVDPEGSSLWIWGLSVLFILAALAVALL
                     IPVATCPSGTSLDPVFRLCIRGSERVPATQWMWAKWAIAIGGIGLGIAIGKLRRIVVV
                     SAIVTVLAWMFALTWLLRETFATTHS"
     gene            803..1246
                     /locus_tag="VIX62_01390"
     CDS             803..1246
                     /locus_tag="VIX62_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858922.1"
                     /translation="MESAKLSYAGIAGAVAGVLGLLGAVSKWYAVSVAGGTLTIKGTS
                     DASGKLALAMSVALFAFSAAYVLIADARIRRSMAALMIVTAVVLTLAAVWGLTRADGA
                     SVEMGLWLTGLAGVLGIGAGVLTLKDAPVDEDIVEPDPVNAEAAS"
     gene            1295..>2520
                     /locus_tag="VIX62_01395"
     CDS             1295..>2520
                     /locus_tag="VIX62_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY5858923.1"
                     /translation="MPDASVQARTWWAREGLENRGGRLTIAGRDAEQLAREHGTPLFV
                     YDLERAREQAELLRDAFEGTGVSALVRFALKAQREPAFLAFLRERAPFVGMDVCSPGE
                     LAWALDHGWAPEEISYTGTNLSDADLARILPTGAHLNVDLLSQLERVGQAEPGRRVGI
                     RVNPRIGATHAGGDESTYAGSKPTKFGIYPERLDDAIEIARRHDLTIDTVHVHSGYLY
                     FSSSLDVVDEMVGRIASITRTLQDRGCPITEVNTGGGLGVPFRPDDEPLDLERWAGIM
                     ARHLGPLGVTVATEPGEFIAKQTGVHLAEVVTVEDRDGTTFVGLDTGWNVLNEHFVYR
                     IPFFPIVCRAADAEPVHRVTVSGHINEGPDLFAEDHPMPEVHEGDIVALPNVGSYNAS
                     MSSAHCLRGPAASIAFP"
BASE COUNT          395 a          876 c          876 g          373 t
ORIGIN      
        1 cagaacgtcc tgcggttggt cgtcgatcat gccgggcctc ctcgaagtgc gagcgggaac
       61 gggttcggac cgccatgcta accgtgtcgg cggtcgtccg tggttcgcgc tcaggagtgc
      121 gtcgtggcga aggtctcccg cagcagccag gtgagcgcga acatccaggc cagcacggtg
      181 acgatcgccg agacgaccac gatgcgccgg agcttgccga tcgcgatccc cagcccgatg
      241 ccgccgatcg cgatcgccca cttcgcccac atccactgcg tggccgggac ccgttccgag
      301 ccgcggatgc acagccggaa caccgggtcg aggctcgtgc ccgaggggca ggtggccacc
      361 gggatcaaga gggcgacggc cagggccgcc aggatgaaca gcaccgaaag cccccagatc
      421 cacaggctcg atccttccgg gtcgaccggc cggcgccaca cggtccaggc gatccggagg
      481 ccggcgccga tcgcggcgag cgagaccagc gagacgagcc cggcggctcc cgcccagtcc
      541 tcgcacgagg gccccttcgc gcaggtgagt tcgtcccacg acgtcacgat gaacgcgatc
      601 cccgccacga tgatgcagcc ggccgtgacg aagccggcga tcctgcccgc cggtgatatc
      661 tctgcacgca tcggcccatc gttccacatg cgatgggcgc aacgcgggtt caagcccagg
      721 ggtggttcct gccgacaacc catcgagagt gagcgctcac tgggtgctcg cgtgcgcacg
      781 aggcgcgacg aagggagctt cgatggagtc ggcaaagctg tcgtacgcgg ggatcgccgg
      841 ggcggtcgcg ggggtcctcg gactgttggg tgcggtatcc aagtggtacg cggtctccgt
      901 ggccggtggc accctgacca tcaagggcac gagcgacgcc agcggcaagc tcgccctggc
      961 gatgtcggtc gcgctgttcg cgttctcggc ggcctacgtg ctgatcgccg acgcccggat
     1021 ccgacgttcc atggcagcgc tgatgatcgt caccgccgtc gtcctgacgc tggccgcggt
     1081 ctggggcctc acgcgcgccg atggcgccag cgtcgagatg ggcctgtggc tcaccggcct
     1141 ggccggcgtg ctcggcatcg gcgcaggcgt gctgacgttg aaggacgctc cggtcgacga
     1201 agacatcgtc gagcccgacc ccgtcaacgc cgaagccgcc tcgtagccca ccgaaccccg
     1261 cgacggcggg gcggacggcc gctagcctgt gcggatgcct gacgcatccg tgcaagcgcg
     1321 aacgtggtgg gcccgcgagg gactggagaa ccgtgggggc cggctgacga tcgcgggccg
     1381 tgacgcggag cagctcgcgc gcgaacacgg cacaccgttg ttcgtctacg acctcgagcg
     1441 cgcccgcgag caggcggagc tcctgcggga cgccttcgaa ggcaccggtg tgtcggccct
     1501 cgtgcggttc gcactgaagg cgcagcgcga gcccgcgttc ctcgcgttcc tgcgggagcg
     1561 cgcacccttc gtgggcatgg acgtgtgttc cccgggcgaa ctcgcgtggg cgctcgacca
     1621 tgggtgggcg cccgaggaga tcagctacac cggcacgaac ctgtccgatg ccgatctcgc
     1681 ccggatcctg ccgaccggcg ctcatctcaa cgtggacctg ctctcgcagt tggagcgcgt
     1741 gggacaggcc gagccgggac gccgcgtcgg catccgggtg aaccctcgca tcggcgctac
     1801 gcacgcgggc ggggacgaga gcacctacgc cgggtccaag cccacgaagt tcggcatcta
     1861 tcccgagcgg ctggacgatg cgatcgagat cgcgcggcgc cacgacctca cgatcgacac
     1921 cgtgcacgtc cactcgggct acctctactt cagctcctca ctcgatgtgg tggacgagat
     1981 ggtcggacgc atcgcctcga tcacccggac cctgcaggac cgcggctgtc ccatcaccga
     2041 ggtcaacacc ggtggcggcc tgggtgtgcc cttccgtccc gacgacgaac cgctggacct
     2101 ggagcgctgg gccggcatca tggcccgcca cctcggtccc ctcggcgtca cggtcgcgac
     2161 cgagccgggc gagttcatcg cgaagcagac cggggtgcac ctggccgagg tcgtgacggt
     2221 ggaggatcga gacggcacca cgttcgtggg actcgacacc ggctggaacg tgctgaacga
     2281 gcacttcgtg taccggatcc cctttttccc gatcgtgtgc cgtgccgccg acgctgaacc
     2341 ggtgcatcgc gtcacggtgt cgggtcacat caacgaaggc ccggacctgt tcgccgagga
     2401 ccacccgatg cccgaggtgc acgagggcga catcgtggcg ctgccgaacg ttggcagcta
     2461 caacgcttcg atgtccagcg cgcattgcct gcgtgggccg gcggcctcga tcgcgttccc
//
LOCUS       DATDBZ010000055         2434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1860529, whole genome shotgun sequence.
ACCESSION   DATDBZ010000055 DATDBZ010000000
VERSION     DATDBZ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860529"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VIX62_01400"
     CDS             <1..659
                     /locus_tag="VIX62_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5858924.1"
                     /translation="SFDAIFEIGRYLAGHVEDHRATRRDDIITHLLDARMPDGRALED
                     PEILGVCILLLLAGIDTTWSAIGASLLHLATHPDDRRRLIEEPDLIHSATEEFLRVFA
                     PVTMAREVIEDTVIGGCPMHTGDRVLLPFPSGNRDPAVFDEPDSVILDREINRHFAFG
                     VGIHRCLGSNLARMELRVTLQTWLRRIPDFTLTDPDAVTWSAGQVRGPRTLPLAFPPA
                     "
     gene            668..1699
                     /locus_tag="VIX62_01405"
     CDS             668..1699
                     /locus_tag="VIX62_01405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY5858925.1"
                     /translation="MKLDIMTGGLPLHVVQQLARHAADSGHSGLVITEAGRTAYLSCA
                     AAALAADIDILTGIAVAFPRSPMVTAQTAWELADATGGRFRLGLGTQVRAHIERRYST
                     LFDPPGPRMRDYVLAVRSCFEAFRSGGTPDHHGPYYDISLLPAMWSPGPIDAPDPPID
                     IAAVNPWMLRLAGEHADGVHIHPLNTRTYLADTVAPQLAAGAQRAGRRLDDFEITVPA
                     FLVVGDTDTDRARWRELARMQVAFYGSTPNYAFIFEQLDHPGTTAALRERQKAGDIAG
                     MATIITDDILRHFTIEGTWATIADGIADRYAGLATRVVNYFGAIAWTEDPQSLARWKP
                     IATALADAP"
     gene            complement(1806..2231)
                     /locus_tag="VIX62_01410"
     CDS             complement(1806..2231)
                     /locus_tag="VIX62_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858926.1"
                     /translation="MNDRIVRVDEHVVVACRPQDALSCLDGPTAIAAWFGGDRSGSRT
                     TIASPAGELVLERAHEEWLPDDGALLVVGTTGDLWFRAHLTVRSVMLPDAHPYLHPGT
                     EIWAHVELGPADRAVQASAVIRDVLRHGLEHLRLELDAS"
BASE COUNT          419 a          984 c          689 g          342 t
ORIGIN      
        1 ccagtttcga tgccatcttc gagatcggca ggtacctcgc gggccacgtc gaggatcacc
       61 gcgcaacacg gcgtgacgac atcatcaccc acctcctcga cgcccgcatg cctgacggcc
      121 gcgccctcga agaccccgag atcctcggcg tgtgcatcct cctgctcctc gccggcatcg
      181 acaccacctg gtccgcgatc ggggcatcgc tcctccacct cgccacccac cccgacgacc
      241 gacgccgcct catcgaagag cccgacctca tccactccgc caccgaggag ttcctgcggg
      301 tgttcgcacc cgtcaccatg gcacgcgagg tcatcgagga caccgtcatc ggcggctgcc
      361 ccatgcacac gggcgaccgg gtcctgctcc ccttcccatc cggaaaccgc gaccccgccg
      421 tcttcgacga acccgacagc gtgatcctcg accgcgaaat caaccgacac ttcgcgttcg
      481 gagtcgggat ccaccgctgc ctgggctcca acctcgcccg catggaactg cgagtgacac
      541 tccaaacctg gctgcgccgc atcccagact tcacgctcac cgaccccgac gccgtcacct
      601 ggtccgctgg ccaagtgcgc ggtccccgca cccttcccct cgcgttccct cccgcatagg
      661 acacgccgtg aagctcgaca tcatgaccgg cggtctccca ctccacgtcg tccaacaact
      721 cgcccgccac gccgccgaca gcggccacag cggcctcgtg atcaccgaag ccggccgcac
      781 cgcctaccta tcctgcgcgg ctgccgcgct cgccgccgac atcgacatcc tcaccggcat
      841 cgcggtcgcg ttcccacgca gccccatggt cactgctcaa actgcgtggg agctcgccga
      901 tgccactggc ggccggttcc gcctcgggct gggaacccag gtccgagccc acatcgaacg
      961 ccgctacagc accctgttcg atccacccgg accacgcatg cgcgactacg tcctcgccgt
     1021 acggtcctgc ttcgaggcct tccgcagcgg cggcaccccc gaccaccacg gcccctacta
     1081 cgacatctcc ctcctgcccg ccatgtggtc acccggcccc atcgacgcgc cagacccacc
     1141 catcgacatc gccgcggtca acccctggat gctgcgcctc gctggcgaac acgccgacgg
     1201 agtccacatc caccccctca acacccgcac ctacctcgcc gacaccgtcg caccacaact
     1261 cgccgctggt gcccaacgag cgggacggcg cctcgacgac ttcgagatca ccgtccccgc
     1321 gttcctcgtc gtcggcgaca ccgacaccga ccgggcccgc tggcgcgagc tcgcccgcat
     1381 gcaagtcgcc ttctacggat ccacgcccaa ctacgcgttc atcttcgaac aactcgacca
     1441 tcccggcacc accgccgccc tacgcgaacg ccagaaggcc ggcgacatcg ccggcatggc
     1501 caccatcatc accgacgaca tcctccgcca cttcaccatc gaaggcacct gggccaccat
     1561 cgccgacggc atcgccgacc gctacgccgg cctcgcgacc cgagtcgtca actacttcgg
     1621 cgccatcgcc tggaccgaag acccccaaag cctcgcccgc tggaagccca tcgccaccgc
     1681 cctcgccgat gccccctagc gcgagcgggt caaaggcgtc gaccgctgac ctcgacatca
     1741 cgtccacaaa cgcgggcacc accgggtttg gcgaagcatc cacccagacg gtgagccacc
     1801 cgcggtcatg aggcgtcgag ctcgagacga aggtgctcga ggccgtgccg gaggacgtct
     1861 cggatcaccg cgctcgcctg caccgctcga tccgcgggtc cgagctccac gtgggcccag
     1921 atctcggtgc ccgggtggag gtacgggtgc gcatcgggca gcatgaccga gcgaacggtg
     1981 aggtgagcgc gaaaccacag gtcgccggtg gtcccgacca ccagcagcgc gccatcgtcg
     2041 ggcagccatt cctcatgggc acgctcgagc acgagctcgc ccgcgggtga ggcgatcgtg
     2101 gttcgagatc cagaccggtc gccaccgaac caggcggcga tcgccgtcgg cccgtccagg
     2161 cagctgagcg cgtcctgggg acgacacgcc acgacgacgt gttcgtcgac gcggacgatg
     2221 cggtcgttca ccctggcctg ctcagagcct cagcgtcgca gtgatgtcgc atgatcggga
     2281 cgggtggcgc gtcagctggg ctcatgagga gcggcgcgtt caggttcggg aggaacgacg
     2341 agcacaggtc ggtcgcaccg gtccacgagg cgctcactca cggtcgagtg tgatccgcgg
     2401 gtgaagcggc gtcgagcgct gcgcccgatg gcga
//
LOCUS       DATDBZ010000056         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2474684, whole genome shotgun sequence.
ACCESSION   DATDBZ010000056 DATDBZ010000000
VERSION     DATDBZ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474684"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VIX62_01415"
     CDS             <1..944
                     /locus_tag="VIX62_01415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5858927.1"
                     /translation="FMPPIDGLAEAQPWDNRDATAAEAVPGSLVVLGGGPIGAELAQA
                     FSRLGARVTMIEGNHRLLSRDEPFAGEQVAAAFVDEGIDVRLGVKAVAVRRDGDGVHV
                     TLDDGAQVDGDEILVAVGRRANTADVGLDTVGLEPGRPISVDERLRVRGVEGEWLYAV
                     GDVSGGAQLTHMGKYQGRVFGDVLAGRDTVDEANARAVPRVTFTDPQIAAVGPTEAEA
                     RERGLDVRVVSYDTGGVAGAYLRGDGVHGTSQLVIDEARGVVVGATFTGPDVQEMLHA
                     ATIAIVGEVPLERLWHAVPAFPTVSEVWLRLLEAYGL"
     gene            956..1399
                     /locus_tag="VIX62_01420"
     CDS             956..1399
                     /locus_tag="VIX62_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5858928.1"
                     /translation="MSVTRIAITMSFVAGLAGAAQASVSGALGKRVGSVQAAAFGAIV
                     AAVLLVGLAVLTGRGPGIADVFRQPAWLLISGVFGAGIVLTLAYAPPRIGTFAAVALM
                     IAGQLLAGALIDGFGWLGTERIPLTAYRVIGLILLTAGAALTLKR"
     gene            complement(1418..2152)
                     /locus_tag="VIX62_01425"
     CDS             complement(1418..2152)
                     /locus_tag="VIX62_01425"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY5858929.1"
                     /translation="MRTSEVEALPIPGVRLRLLGCFELSHGQAIARLPMLAQRLATLL
                     AIRGETMDRSRAAGTLWPEVDTHHAMANLRTALWRLRSTRFHIVEPIGQALRLSPGVE
                     VDLRDREDLAHRALRGELPRDEARGASAALSQDVLLDWDDEWLVFDRERFHELRLHAL
                     ERLCDQLAAWGDHAEAVECALLAIEAEPLRESAHRALMRAHIAEGNHARAMQHLHSLT
                     RQLWQELEVEPSQETIDLARRPRLSG"
BASE COUNT          355 a          869 c          904 g          367 t
ORIGIN      
        1 cgttcatgcc accgatcgac gggctggccg aggcgcagcc ctgggacaac cgggacgcga
       61 ccgccgcgga agcggtcccg gggagcctcg tcgtgctggg aggcggtccg atcggcgccg
      121 agctcgcgca ggcgttctcc cggctgggcg ccagggtcac gatgatcgaa gggaaccacc
      181 gattgctgtc gcgtgacgag cccttcgccg gcgagcaggt cgccgccgcg ttcgtcgacg
      241 aaggcatcga cgtgcggctc ggggtgaagg cggtcgcggt gcggcgcgac ggcgacggcg
      301 tgcacgtcac gctcgacgac ggcgcgcagg tggacggcga cgagatcctc gtcgccgtcg
      361 ggcgccgcgc caacaccgcc gacgtcggcc tcgacacggt cgggctcgag ccgggccgtc
      421 cgatctccgt cgacgaacgg ctccgcgtcc gtggtgtcga aggggagtgg ctctacgcgg
      481 tgggcgacgt gtcgggcggc gcgcagctca cccacatggg caagtatcag ggccgggtgt
      541 tcggcgacgt gctcgcaggg cgcgacaccg tcgacgaggc gaacgctcgt gcggtgccca
      601 gggtcacctt cacggacccg cagatcgcgg cggtggggcc gaccgaggcc gaggcccgtg
      661 agcggggcct cgacgtgcgg gtcgtgtcct acgacaccgg gggcgtcgcc ggcgcctacc
      721 tacgcggtga cggcgtccac ggcaccagcc aactcgtgat cgacgaggcc cgaggtgtgg
      781 tcgtgggcgc gaccttcacc ggacccgacg tgcaggagat gctccacgcc gccacgatcg
      841 cgatcgtggg cgaggtgccc ctggagcgac tgtggcacgc ggtccccgcg ttcccgacgg
      901 tcagcgaggt ctggctgcgg ttgctggagg cgtacggcct gtagcctgca gcggtatgtc
      961 cgtcacccgg atcgcgatca cgatgtcgtt cgtggcggga ctcgccgggg ccgcgcaggc
     1021 gtcggtcagc ggggccctcg gcaaacgggt gggaagcgtg caggcggccg cgttcggcgc
     1081 gatcgtggcc gccgtgctgc tggtcggact cgcggtgctg acggggcggg gcccgggcat
     1141 cgccgacgtc ttccgtcagc ccgcgtggct gttgatctcg ggggtgttcg gcgccggcat
     1201 cgtgctgacg ctcgcgtacg cgcccccgcg gatcggcacg ttcgccgccg tcgccctgat
     1261 gatcgccggg caactgctcg cgggcgctct gatcgacggg ttcgggtggc tcggcaccga
     1321 gcgcatccca ctgaccgcct acagggtgat cggactgatc ctgttgaccg cgggggcggc
     1381 gctcaccctg aagcgctgag cgtggtcccc cgatcgctca accgctgagg cgtgggcgtc
     1441 tcgccaggtc gatcgtctcc tgggagggct ccacctcgag ctcctgccac agctgacgcg
     1501 tgagcgagtg caggtgctgc atcgccctcg catggttgcc ctcggcgatg tgcgcccgca
     1561 tcaaggctcg gtgcgcagat tcccgcagcg gctccgcctc gatcgcgagc aacgcgcact
     1621 cgaccgcctc cgcgtggtcg ccccacgccg ccagctggtc gcacaaccgt tccagggcgt
     1681 gcagccgcag ctcgtggaag cgctcccgat cgaagaccag ccattcgtcg tcccagtcga
     1741 ggagcacgtc ctggctgagg gcggcggatg ccccgcgagc ctcatcgcga ggcagttcgc
     1801 cgcgcagcgc ccggtgagcc agatcctcgc ggtcgcgcag gtcgacctcg acccccggcg
     1861 acaggcgcag ggcctgaccg atcggctcca cgatatggaa ccgcgtgctc cgcagtcgcc
     1921 acagggccgt gcgcaggttc gccatcgcgt gatgcgtgtc gacctcgggc cacagggtgc
     1981 cggcggccct cgatcgatcc atcgtctcgc ctcggatggc cagcagcgtc gccaatcgct
     2041 gcgccagcat cggcagccgg gcgatcgcct ggccgtggga gagctcgaag cagccgagca
     2101 atcgcagccg gacccccgga atcgggagcg cctcgacttc cgacgttctc acccgcacag
     2161 cgatcaccaa tcctcttcag gggggaaggc cctgaagacg ctcgatccga gcgggcctgc
     2221 actgcccgtt cggacgcccg atcgcctccg ggtgttgcct gtaacccgta cccgtgcgtt
     2281 ctacctcgaa gcaacgtgag gatgcaagtg gcagttcaat cgtgctcagg aggtgacacg
     2341 gcgaacgttg gggcctgaca gccgacgaag cgttgacgat gcgaagacgc aacgttgacg
     2401 ccgcagccac gcacgagccc caccctctcc tcaggtggtc cggggcgccg ccccgccgac
     2461 gtcggcggaa agcccgttcg agagggggaa cacat
//
LOCUS       DATDBZ010000057         4613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2474690, whole genome shotgun sequence.
ACCESSION   DATDBZ010000057 DATDBZ010000000
VERSION     DATDBZ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474690"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1392
                     /locus_tag="VIX62_01430"
     CDS             40..1392
                     /locus_tag="VIX62_01430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5858930.1"
                     /translation="MSDADVIVMGMGPGGEEVAGRCTDGGLRVLGIERKLVGGECPYW
                     GCVPSKVMVRAADSLAEAARAIGLAGSLRIDPEWAKVAARVREFTADWNDEAAVERHE
                     AKGTKIMRGAARLRGPRSVEVDGAVYEAERGVVIATGGEPAIPPIEGLNETPYWTNRG
                     AIEATDVPGRLIVLGGGAIGLELAQVFHRFGADVTVVESAARVLALEEPENGEALGRV
                     LSDEGITLRLGVRAERVSSAGDGVRVALSDGSGVEGDRLLVATGRRSDLEAVGVAAAG
                     LDPSARSIEVDDRMRVADGLWAVGDITGKGGFTHIATYQGRIAAADILGQPHAPADYS
                     AVPRVTFTDPEVASVGLTQQQAEDAGILVQVGVSDTSYSTRGSIHGTNAELGVIKLIA
                     DTDRGVLVGGSIMSPAAGEMLGIVMLAVRAQVPIQTLKDLIYPYPTFIRGLESSLAEL
                     "
     gene            complement(1402..2790)
                     /locus_tag="VIX62_01435"
     CDS             complement(1402..2790)
                     /locus_tag="VIX62_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEY5858931.1"
                     /translation="MPTPRTPQYLSVEERRAQGKRARERAPRSSIATWRPAADRPDPV
                     ATLTEQDETREPDLVPIRHGRMMVSPFTFFRGGARIMAWDLASVATSGLKVQLCGDAH
                     LSNFGVFASPERQLLFDINDFDETLPGPFEWDVARLAASFVIASRNNGFTEEEARVSA
                     VAAVTAFREAMAGFAQMRTLEIWYAHLPAEEILASVRTPRLKESIQRGLDHSRTRDSL
                     QALTKLAEMVDGHYRIVTRPPVVMPIRDLAHLSEEEVQAGIHEMLHEYRRSLPDERRH
                     LLERYRFTDAAHKIVGVGSVGLRAFIALLQGRDEDDPLFLQMKEARHSVLEEHLPKSR
                     FRNHGQRVVEGQRLMQAASDIFLGWTKGIDRQRNYYWRQLRDMKGSLDVEHAPPGVMT
                     LYARTCGWTLARAHARSGDPVAIAAYLGVSDRFDRAMGDFAERYADVNDGDHREFCSA
                     IATGKLPVREGV"
     gene            2825..3757
                     /locus_tag="VIX62_01440"
     CDS             2825..3757
                     /locus_tag="VIX62_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5858932.1"
                     /translation="MASPSSRADEASAELTHRGGGIVAVSITTVIWGLVPLVFKETDM
                     PTLAFATYRLWMGVLVYAVVFAFTGRRVRWSVMRVCALGGVFFAFDIAFTFSAFRLTS
                     IANATIIGALAPVFITLGAARWFGERVERRDLLFVGMSFAGVAMVALGSAGSPTWSPL
                     GDAFAAASVLSWTCYWLFSKRARARTEVGALEYMACVMTVAAVIMTFLALAFGQSLSW
                     PSRADWGWIWLVTIFPGFIGHSLISWSHRHLEAWLGSLITQLQPVIATAAGWVLLSEA
                     PTELTIAGGLVVVAATAAIMIRAGRQSVSIGEGL"
     gene            complement(3727..4155)
                     /locus_tag="VIX62_01445"
     CDS             complement(3727..4155)
                     /locus_tag="VIX62_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006142557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5858933.1"
                     /translation="MPAKRVEELLQDARARLQRITPEQAHAEQRDHGALIVDTRTFEQ
                     RRAQGLIPGALVIDRTVFEWRLDPAGEHHVAGVTDHDVRIIVICRQGYSSSLAAASLH
                     DLGLVRATDVIGGFDAWRAAGLPVEAFDPDTYRPSPIDTD"
     gene            4262..>4613
                     /locus_tag="VIX62_01450"
     CDS             4262..>4613
                     /locus_tag="VIX62_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019749472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5858934.1"
                     /translation="MTDRDAAADALAGRIFGAVLETFDIYAVYIGDRLGYYRALATDP
                     LTSTELATRTGTAERYAREWLEQQAVTGILVTDDRVVDALARRYALPAAHVGPLTDAL
                     GPSHFAPFVQFVVGC"
BASE COUNT          648 a         1571 c         1634 g          760 t
ORIGIN      
        1 ctaccgtggg cacgggcctc cgaacggaag gatcagggtg tgagcgacgc cgacgtcatc
       61 gtgatgggga tgggccccgg aggcgaggag gtcgccggtc ggtgcaccga tgggggcctc
      121 cgcgtcctgg gcatcgaacg caagctcgtg ggaggcgaat gcccctactg gggctgcgtg
      181 ccctcgaagg tcatggtgcg cgccgccgat tccctcgccg aagcggcgcg tgccatcggg
      241 ctggccggct cgttgcgcat cgatcctgag tgggcgaagg tcgcggcgcg cgtccgggag
      301 ttcaccgccg actggaacga tgaagcggcg gtcgagcggc acgaggcgaa aggcacgaag
      361 atcatgcgtg gcgcggcccg gctccgcggg ccacgttccg tcgaggtcga cggcgccgtc
      421 tacgaggcag agcgcggggt cgtgatcgcc accggcggcg aacccgcgat cccacccatc
      481 gaaggcctga acgagacgcc ctactggacc aaccgtggtg cgatcgaggc gaccgacgtg
      541 cccggccgac tcatcgtgct cggaggtggc gcgatcggct tggaactggc gcaggtgttc
      601 caccggttcg gcgccgacgt caccgtcgtg gagtccgccg cccgcgtgct cgcgctggag
      661 gaacccgaga acggcgaggc gctcggccgc gtcctgtcgg atgaagggat cacgctccgc
      721 ctcggcgtcc gggccgaacg agtctcgtcg gcgggcgacg gcgtgcgcgt ggccctgtcc
      781 gatggctccg gggtcgaagg ggaccggctc ctcgtcgcca cgggacggcg cagcgatctc
      841 gaggcggtcg gcgtggctgc cgccgggctc gacccttcgg ctcgctcgat cgaggtggac
      901 gaccggatgc gcgtggccga cggcctctgg gcggtgggcg acatcaccgg caagggcggg
      961 ttcacccaca tcgccaccta tcaggggcgg atcgcggccg ccgacatcct cggccaaccg
     1021 cacgcaccgg ccgactactc ggcggtgcct cgggtcacgt tcaccgatcc agaggtggcg
     1081 agcgtggggt tgacccagca gcaagccgaa gatgccggga tcctggtgca ggtcggcgtc
     1141 tccgacacca gctattccac ccgcgggtcc atccacggga cgaacgccga gctcggcgtc
     1201 atcaagctga tcgccgatac cgatcgcggc gtcctggtgg gcggttcgat catgagcccg
     1261 gccgccggcg agatgctggg catcgtgatg ctggcggtgc gcgcccaggt gccgatccag
     1321 acgctgaagg atctgatcta cccctatccg acgttcatcc gcggattgga gtcatcgctg
     1381 gccgagctgt gagcccggcg atcacacgcc ttcgcggacc ggcagcttcc cggtggcgat
     1441 cgcgctgcag aactcgcggt gatcaccgtc gttcacgtcg gcgtaccgtt cggcgaagtc
     1501 gcccatggcg cggtcgaacc gatcgctgac cccgaggtag gcggcgatcg ccactgggtc
     1561 gccggagcgc gcatgcgcac gggccagcgt ccatccgcac gtgcgggcat acagcgtcat
     1621 cacgccgggc ggcgcgtgct ccacatcgag cgaacccttc atgtcgcgga gctgtcgcca
     1681 gtagtagttg cgttgtcggt cgatgccctt cgtccacccg aggaagatgt cgctggcggc
     1741 ctgcatcagc cgctggccct ccaccacgcg ttgcccgtgg ttgcggaagc gtgacttcgg
     1801 caggtgctcc tccaacaccg aatggcgtgc ctccttcatc tgcaggaaca acgggtcgtc
     1861 ttcgtcgcgc ccctgcagca gggcgatgaa cgcccgcagc cccacgctgc cgacaccgac
     1921 gatcttgtgc gccgcgtcgg tgaagcgata gcgctccagg aggtggcgtc gctcgtcggg
     1981 aagcgaccga cggtactcgt ggagcatctc gtggatgccc gcctgcacct cctcttcact
     2041 caggtgggcg aggtcgcgga tcggcatgac caccgggggc cgcgtcacga tccggtagtg
     2101 cccgtcgacc atctcggcca gcttcgtgag cgcctggagg ctgtcccgcg tcctcgagtg
     2161 gtccaaccct cgttggatcg actccttcag tctcggcgtg cgcaccgagg cgaggatctc
     2221 ctcggccggc aggtgcgcgt accagatctc caacgtgcgc atctgcgcga agcccgccat
     2281 cgcctcccgg aaggccgtga ccgcggcgac ggccgagacg cgcgcctcct cttcggtgaa
     2341 cccgttgttg cgcgaggcga tgacgaagct cgccgcgagc cgcgccacgt cccactcgaa
     2401 cgggccgggg agcgtttcgt cgaagtcgtt gatgtcgaac aacagctggc gctccggtga
     2461 ggcgaacacg ccgaagttgg aaaggtgggc gtcgccgcag agctggacct tcagcccgga
     2521 cgtggccacg ctcgccagat cccacgccat gatcctggct ccgccccgga agaacgtgaa
     2581 gggcgagacc atcatccggc cgtggcggat cggcaccagg tcgggttcac gcgtctcgtc
     2641 ctgctcggtc agggtcgcga cggggtcggg gcgatcggcg gccggtcgcc acgttgcgat
     2701 gctggagcgg ggcgcgcgtt cgcgggcccg cttgccctgg gctcggcgct cctcgacgga
     2761 taggtactgc ggcgttcggg gcgtgggcat gggggtaacc gtagtcgctg atagcgtccc
     2821 cggcatggca tctccgtcat cccgcgctga cgaggcttcc gccgaactca cccatcgcgg
     2881 cggcggcatc gtcgccgttt cgatcaccac cgtgatctgg ggactggtgc cgctggtctt
     2941 caaagagacc gacatgccca cgctggcatt cgccacctat cgcctgtgga tgggcgtgct
     3001 ggtctacgcg gtggtgttcg ccttcacggg acggcgggtg cgctggtcgg tgatgcgggt
     3061 gtgcgcgctg ggcggggtgt tcttcgcctt cgacatcgcg ttcacgttca gcgccttccg
     3121 gctgacgagc atcgcgaacg ccacgatcat cggggcactg gcgccggtgt tcatcacgct
     3181 gggtgcggcg cggtggttcg gcgaacgtgt ggagcgtcgc gacctcctgt tcgtggggat
     3241 gtccttcgcg ggcgtggcca tggtggcgct cggctccgcc ggctcgccca cgtggagccc
     3301 gctcggtgat gcgttcgccg ccgcgagcgt gctgagttgg acctgctact ggctcttctc
     3361 caagcgggcg cgcgcccgca ccgaggtcgg cgccctcgag tacatggcgt gcgtgatgac
     3421 cgtggccgcg gtgatcatga cgttcctggc gctggcgttc ggtcagtcgc tctcgtggcc
     3481 ctcgcgcgcc gactggggat ggatctggct cgtcacgatc ttccccggct tcatcgggca
     3541 ctcgttgatc tcgtggtcgc accgccacct cgaagcgtgg ctgggttcgc tcatcaccca
     3601 gctgcagccg gtgatcgcca ccgccgcggg gtgggtcctg ttgtccgagg ctcccacgga
     3661 gctgacgatc gccggcgggc tcgtcgtggt ggcggcgacc gcggccatca tgatccgtgc
     3721 cggccgtcag tcggtgtcga tcggcgaggg tctgtaggta tccggatcga aggcctcgac
     3781 gggcaggccg gcggctcgcc aggcgtcgaa cccgccgatc acgtcggtgg cccggaccag
     3841 accgaggtcg tgcagcgagg ccgcggccag cgatgaactg tagccctgac gacagatcac
     3901 gatgatgcgc acgtcgtggt cggtgacgcc ggccacgtgg tgctcgcccg cggggtcgag
     3961 ccgccactcg aacaccgtgc gatcgatcac cagcgccccc gggatcaacc cttgcgcgcg
     4021 ccgctgctcg aaggtgcggg tgtcgacgat caacgcgcca tggtcacgct gttcggcgtg
     4081 cgcctgctcg ggggtgatgc gctgcaaccg cgcgcgggca tcctgcagca gctcctccac
     4141 acgcttggcc ggcatccccg aatcctggca cacaccgtgg ccggcatcct ccgaacgtgc
     4201 gaggagaccc gggcgcgctc gcccgactag gctcgtccgg acgacggagg aaggaacagt
     4261 catgaccgat cgtgacgcgg cggcggacgc ccttgcgggc aggatcttcg gggcggtact
     4321 ggagaccttc gacatctatg ccgtctacat cggagaccgc ctcggctact accgggcgct
     4381 cgccacggat cccctcacgt ccaccgagct ggccacgcgc accggcaccg ccgagcggta
     4441 cgcccgcgag tggctggaac agcaggcggt gaccgggatc ctcgtgacgg atgatcgggt
     4501 cgtcgacgcg ctggcccggc gctacgcgct gccggccgcg cacgtcggac cgctgaccga
     4561 cgccctcggc ccctcgcact tcgctccgtt cgtccagttc gtcgtcggct gtc
//
LOCUS       DATDBZ010000058         4771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2679170, whole genome shotgun sequence.
ACCESSION   DATDBZ010000058 DATDBZ010000000
VERSION     DATDBZ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679170"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..267)
                     /locus_tag="VIX62_01455"
     CDS             complement(166..267)
                     /locus_tag="VIX62_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEY5858935.1"
                     /translation="MGSLVKKRRKKMRKKKHKKLLKRTRHQRMKLGK"
     gene            complement(285..1196)
                     /locus_tag="VIX62_01460"
     CDS             complement(285..1196)
                     /locus_tag="VIX62_01460"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HEY5858936.1"
                     /translation="MDDGGSVVNEHDRLVDRAAREARTVVFIGGLDAGKSTLARATAA
                     YALRRGRTVAYLDADVAQKTVGPPATVGLKHIREPDDLTLERLSHADALGFVGSTTPQ
                     DHLLPLVGALARLRDRAREEGSDLVVVDTSGMVSGIWGQLVKYYKIDLLEPDLVVGLQ
                     RGGELDPILGVIERFFGIDAVSLPIHPGVVPTSVEQRSEQRERAMASYFQGDLQRFRV
                     KPTVFMPTLPELFDLRELDRLLVGLSDGQGGFTGLGYLEFVGEDGGLRLISPVAAPPK
                     ALRLGSVRLEASFRVKRIDLRNLFGTE"
     gene            complement(1247..1444)
                     /locus_tag="VIX62_01465"
     CDS             complement(1247..1444)
                     /locus_tag="VIX62_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5858937.1"
                     /translation="MRREPFTEARLLTVNEVADLLRVSRMTVYRLIKTGDMPALRVGR
                     SYRLREEDVHTYLRERYTEAG"
     gene            complement(1539..2369)
                     /locus_tag="VIX62_01470"
     CDS             complement(1539..2369)
                     /locus_tag="VIX62_01470"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEY5858938.1"
                     /translation="MDVIGAHVRRLPPLDDRWWPHAQDAAVLAEALLEGGVAGTATHP
                     LDNVRGNALMLLEGDPDKLFGFDADLPAGLDLDDVLAIVETGAGAAIDRDARYGPVDI
                     RPEPILAACAALGDRLAEACRRGESVIVATGHPVGLALLYQAIDGLVTERGARVITPA
                     DGARWRDPFLHHDWTIEHWNGVGMLTDGHEPRHTHRPDAMRTMLTEATPDLVIADHGF
                     AGAAIAAGIETVSIADVNDPALLVAQAKGLTDTVLVMDDHVDPSAYWPCFQAIAARLD
                     "
     gene            2407..3195
                     /locus_tag="VIX62_01475"
     CDS             2407..3195
                     /locus_tag="VIX62_01475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5858939.1"
                     /translation="MELKQAIVVVTGASAGIGKATAVAFAQRGATVVLAARRLDRLES
                     LADRIERAGGSALALKVDVTDPREVDGLPSLIREVYQRPADVLINNAGIRGGPIQDLS
                     YEQIRKVVDVNLLGVLYGTRAFLPQMLRDGRGHIVNVASLAGRFASPGTGVYSATKHA
                     VVAFSESTNYDAEGRGVHVTAVNPGFIDTEGFPQDDLPSWMVMRVERVAEAIVKVVRD
                     EIAPELSVPRWVSPLQAFRVLTPPLYRWGVRRARRAGTARTKQP"
     gene            3192..3491
                     /gene="clpS"
                     /locus_tag="VIX62_01480"
     CDS             3192..3491
                     /gene="clpS"
                     /locus_tag="VIX62_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY5858940.1"
                     /translation="MSTAQTAPVEVERPGSDEAIEPDVPWIVIVWNDPINLMVYVTWV
                     FQKLFGYSRAKAEKLMMDVHVKGKAVVSNGSRDEAERDVARLHSYGLWATMQQDR"
     gene            3488..3982
                     /locus_tag="VIX62_01485"
     CDS             3488..3982
                     /locus_tag="VIX62_01485"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEY5858941.1"
                     /translation="MNLSGPIRREGEGRFALRLGAKDREAIVAFTAQLRQILVNESAS
                     SDPAVARLFPAAHPDDIFRNLEFERTHAGSLLTERLEALDVVDRTAQADVLTEDELLA
                     WLRSLNALRLVLGTRLDVTEDSTEADFAGNDEAAGAFDLYGYLTWLEAAAVEALSTDL
                     GDPA"
     gene            complement(3979..4152)
                     /locus_tag="VIX62_01490"
     CDS             complement(3979..4152)
                     /locus_tag="VIX62_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858942.1"
                     /translation="MGRTPADFPGRPGKDVVPTKREHMDLDEKATRHRDMEAAHPHGL
                     PWYKRLFRRSKPG"
     gene            complement(4175..>4771)
                     /locus_tag="VIX62_01495"
     CDS             complement(4175..>4771)
                     /locus_tag="VIX62_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY5858943.1"
                     /translation="QMLESMIRAARPTRAEASDVANAVLDGADGIMLSGETAIGEYPF
                     EAAAAATKIAEAVEARASEFRSGEPPCRHTGEAAAVAHAVADVASSEAEIVAITCYTE
                     TGRTARLLSAERPSCPIYAFIPEAGVRRVSTLHWGVTPLAAERPADTDEMIETMDRGL
                     LQHDLARAGQSVVMAAASPSGRTTTNMLKVHQVGSPIR"
BASE COUNT          727 a         1593 c         1671 g          780 t
ORIGIN      
        1 aggggaacgt gaccgcctgt gaccggtcga gcgcgaaccg ctgcacgtcg gcggcgcgat
       61 caccgcacgc cgacaacgcg agcgaggtca ccacgaatcc ggcgatcaac cgagcgatcg
      121 gggaccaggc caggatccgg acgggccgca cggaggggtc cggcgctact tgcccagctt
      181 catgcgctgg tgccgcgtgc gcttgaggag cttcttgtgc ttcttcttgc gcatcttctt
      241 gcggcgcttc ttgaccaaag accccaaacg tcaagccctt tcgttcattc ggttccgaag
      301 agattccgga gatcgatccg cttcacgcgg aagctcgcct ccagacggac ggagcctagt
      361 ctaagcgctt tcggcggcgc tgccaccggt gagatcaggc gcagcccgcc gtcctcgccc
      421 acgaactcga ggtagccgag cccggtgaac cctccctggc cgtcggagag ccccaccagg
      481 agacggtcga gttcgcgcag atcgaacagt tcgggcagcg tgggcatgaa caccgtgggc
      541 ttgacgcgga accgctgcag atcgccctgg aaataggacg ccatcgcccg ttcgcgctgc
      601 tccgagcgct gctcgacgct ggtggggacc acgcccggat ggatcggcag cgagaccgcg
      661 tcgatcccga agaagcgctc gatcaccccg aggatggggt cgagttcgcc gccacgctgc
      721 agccccacga ccagatccgg ttcgaggaga tcgatcttgt agtacttcac cagctggccc
      781 cagatgccgc tcaccatgcc gctggtgtcg acgaccacga gatcgctccc ctcctcgcgt
      841 gcgcggtcgc ggagccgggc gagcgctccc accaacggca gcaggtggtc ctggggtgtc
      901 gtcgatccca cgaacccgag cgcgtcggcg tgggagagcc gttccagcgt gaggtcgtcg
      961 ggctcacgga tgtgtttcag gcccacggtg gcggggggtc cgaccgtctt ctgggcgacg
     1021 tcggcgtcga ggtaggccac ggtacgacct cgccggaggg cataggcggc ggtggcacga
     1081 gccaaggtgc tcttgcccgc gtcgaggccg ccgatgaaca cgacggtgcg tgcctcccgg
     1141 gcggctcgat cgacgagccg gtcgtgctcg ttcacaacgc tccctccgtc atccacggag
     1201 gttcggtcgc gacgtgtgcg tcaggaagtg acgggcgggc gcggcatcag ccggcctcgg
     1261 tgtagcgctc cctcaggtag gtatggacgt cttcctcgcg gagccggtag ctcctgccca
     1321 cccgcaaggc gggcatgtct cctgtcttga tcagtcggta gacggtcatc ctggatacac
     1381 gcaagaggtc tgcgacctcg ttcacggtca acaatcgagc ctcggtgaag ggttctctcc
     1441 tcacgatggg cgcgattgta cgggcacggg cctatgcctg gatagccccc gttcgggccc
     1501 ttgtggttgg gggtggtagc gcttgcgccc gaacatcttc agtcgaggcg ggctgcgatg
     1561 gcttggaagc agggccagta ggcgcttggg tcgacgtggt cgtccatgac caggaccgta
     1621 tcggtgagcc ctttggcttg cgctaccagg agggcggggt cgttgacgtc ggcgatgctc
     1681 accgtttcga tgccggcggc gatggcggcg ccggcgaagc catggtcggc gatgacgaga
     1741 tcgggggtgg cctcggtgag catcgtgcgc atcgcgtcgg gccggtgggt gtggcgaggt
     1801 tcgtggccgt cggtgagcat gcccaccccg ttccagtgct cgatcgtcca gtcgtggtgc
     1861 aggaacgggt cccgccaccg ggcgccgtcg gcgggagtga tcacgcgggc gcctcgttcg
     1921 gtgacgagac cgtcgatcgc ctggtagagg agcgcaagtc ccacagggtg accggtggcc
     1981 acgatcaccg attcgcctcg tcggcacgcc tccgcgaggc gatcgcccag cgcggcgcat
     2041 gccgccagga tcggttcggg acggatgtcg accggcccgt atcgggcatc ccggtcgatg
     2101 gccgctccgg ccccggtctc cacgatcgcg aggacgtcgt cgaggtccag ccctgccgga
     2161 agatccgcgt cgaacccgaa cagcttgtcg gggtcgccct ccagcagcat gagcgcattc
     2221 ccgcggacgt tgtccagcgg atgggtggcg gtgcccgcca ccccgccctc cagcagggct
     2281 tcggcgagca ccgccgcgtc ctgtgcgtgc ggccaccacc gatcgtccag cggtggcagt
     2341 cgccggacgt gtgctccgat aacgtccatg ggtccagcgt agaggagccc acacgatgag
     2401 gaccccgtgg aactgaagca ggcgatcgtc gtcgtcaccg gagcctccgc cggcatcggt
     2461 aaggccaccg cggtggcctt cgcccagcgg ggagccaccg tggtgctcgc ggcccgccgc
     2521 ctcgatcgac tggaatccct ggccgaccgg atcgaacggg cgggtggctc ggcgctcgcc
     2581 ctcaaggtcg acgtcacgga tccgcgagag gtggacggtc ttccgagcct gatccgcgag
     2641 gtctaccagc gccccgccga cgtgttgatc aacaacgccg gcatccgcgg aggaccgatc
     2701 caggatctgt cctacgagca gatccgcaag gtggtcgacg tgaacctact gggcgtcctc
     2761 tacggcacgc gagcgttcct gccgcagatg ctgcgagatg gtcgcggcca catcgtcaac
     2821 gtcgcctcgc tcgcggggag gttcgcgtca cctggcaccg gcgtgtactc ggccaccaag
     2881 cacgcggtgg tggcgttcag cgaatccacg aactacgacg cggagggccg gggcgtgcat
     2941 gtcaccgcgg tcaatcccgg cttcatcgac acggagggct tcccccagga cgacttgccg
     3001 tcatggatgg tgatgcgggt cgagcgggtg gccgaggcca tcgtgaaggt cgttcgcgac
     3061 gagatcgcgc cggagctgag cgtcccccgg tgggtgagcc ccctgcaggc attccgcgtc
     3121 ctcaccccac cgctgtaccg ttggggggtt cgccgggccc gtcgagccgg gacggcgagg
     3181 acgaagcagc catgagcacc gcgcagacgg ctccggtcga ggtcgagcgc cccggttccg
     3241 acgaggcgat cgagccggac gtgccgtgga tcgtcatcgt gtggaacgat ccgatcaacc
     3301 tgatggtcta cgtcacgtgg gtgttccaga agctcttcgg ctactcacgg gccaaggccg
     3361 agaagctgat gatggacgtc cacgtcaaag gcaaggccgt cgtgtcgaac gggtcgcgcg
     3421 acgaggccga acgcgacgtg gccaggctcc attcctacgg cttgtgggcc acgatgcagc
     3481 aggaccgatg aacctgtccg gcccgatcag gcgcgagggc gaggggcggt tcgcgctgcg
     3541 tctgggtgcc aaggaccgcg aggccatcgt ggcgttcacg gctcaactcc gccagatcct
     3601 ggtgaacgaa agcgccagca gcgatccggc ggtggcccgc ctgttccccg ccgcgcatcc
     3661 cgacgacatc ttccgcaatc tcgagttcga gcggacgcac gcgggttcgc tgctcaccga
     3721 acgcctcgaa gcgctcgacg tcgtcgaccg cacggcgcaa gcggacgtgc tcaccgagga
     3781 cgaactgctg gcgtggctcc ggtcgctcaa cgccctgcgc ctggtgttgg gtacccggct
     3841 ggacgtcacg gaggacagca ccgaggccga tttcgcgggc aacgacgagg cggccggagc
     3901 cttcgacctc tatggctacc tcacgtggct ggaagccgcg gcggtcgagg cgctctcgac
     3961 cgacctggga gaccccgcct agcccggttt gctgcgtcgg aagagtcgct tgtaccaggg
     4021 gagcccgtgc ggatgcgccg cttccatgtc gcgatgccgg gtggccttct cgtccagatc
     4081 catgtgctcc cgcttcgtgg gaacgacgtc cttgccgggg cgtccgggga aatctgcggg
     4141 ggttctgccc atggttggcc ctcctcgcgt gggctcagcg gatcggggag cccacctgat
     4201 ggaccttcag catattcgtc gtggtgcgac cggaggggga tgccgccgcc atcaccacgg
     4261 attgcccggc ccgcgcgaga tcgtgctgca gcaggccacg gtccatcgtc tcgatcatct
     4321 cgtcggtgtc ggcgggtcgt tcggcggcca agggggtgac gccccagtgc aaggtgctga
     4381 cccggcgcac gcccgcctcg gggatgaacg cgtagatcgg gcacgacggc cgttcggccg
     4441 acagcagccg ggcggtgcgg ccggtctcgg tgtagcaggt gatcgccacg atctcggctt
     4501 cgctcgaggc gacgtcggca acggcgtggg cgacggccgc cgcttcgccg gtgtgccggc
     4561 acggcggctc ccccgatcgg aactccgacg cccgcgcctc gacggcctcg gcgatcttcg
     4621 tcgccgccgc ggccgcttcg aaggggtact caccgatcgc ggtctcgccc gagagcatga
     4681 tcccgtccgc gccgtccagc accgcgttgg cgacgtcgga cgcttcggcg cgcgtcggcc
     4741 gggcagcgcg gatcatcgac tccagcatct g
//
LOCUS       DATDBZ010000059         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1451483, whole genome shotgun sequence.
ACCESSION   DATDBZ010000059 DATDBZ010000000
VERSION     DATDBZ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451483"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VIX62_01500"
     CDS             complement(<1..667)
                     /locus_tag="VIX62_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY5858944.1"
                     /translation="MSTGSYHAIVVGGGHNGLVAGAYFAKSGARTVVLEARPKVGGAV
                     DTSAPFADHPEIKVSTYSYVMSLMPAFLIEELQLAKHGYQVTPFGPYYQAFPDGRSIT
                     IYGDDPAKTHASVSQFSVKDADTLAAYEAWIHGVTEVVGPLLHQVPPNLGSMTVGDLL
                     EQSKAAWLMRALGTRGIADATRLFTMSLSELLDRWFESEQMKTIMMVDGLIGQWSGPD
                     EPGT"
     gene            complement(664..1836)
                     /locus_tag="VIX62_01505"
     CDS             complement(664..1836)
                     /locus_tag="VIX62_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5858945.1"
                     /translation="MQIDLTGADVIDAHCHPFRTQDLLDREPRTFETRCMFLGTALIS
                     SNHANLEAAAFVEELTETTMFGLALRRWIAEHLGCEPTKDAVVAARDAALRADPPGYV
                     RGLLESEHIVAVVADEGYPQPTITRDEFESALGGVPVHRVGRIEPWIVEAREEGTFDG
                     LVQRFEDLANGAADDPRLIGFKTIIAYRTGLDITDPSTSDCVAAFERWRADDWRESRE
                     HAKPVRDFLLRRAFDIAKERDRVFHVHVGAGDPDVNLTHAKPSDAFSFFVEHQEQPIL
                     MIHSGWPWVAEATYIPTVLPNVYMDVSELVPWGWGQIDWSLEMILGAVPAAKVVHGCD
                     ESSEPEMFPVSARLVRQSLERVLGAFVDRDFLSVPDAESIGRGVLADNVRKLHGLS"
     gene            complement(1866..>2066)
                     /locus_tag="VIX62_01510"
     CDS             complement(1866..>2066)
                     /locus_tag="VIX62_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY5858946.1"
                     /translation="VKVARGIHQLPASLAEAAERLAASDVLRAAMGDFLFETFVATRR
                     GECAQYEGLDDDEMIRRLRWRY"
BASE COUNT          327 a          743 c          674 g          322 t
ORIGIN      
        1 cggtgccggg ctcgtcgggc cccgaccact gcccgatcaa gccgtcgacc atcatgatgg
       61 tcttcatctg ctcggattcg aaccaacggt cgagcagctc ggacaggctc atcgtgaaca
      121 gccgcgtggc gtcggcgatc ccgcgcgtgc ccagcgcccg catgagccac gccgccttcg
      181 actgctccag caggtccccc accgtcatcg atccgaggtt gggcggcacc tggtgcagca
      241 gcggtcccac cacctcggtg acgccgtgga tccacgcctc gtaggcggca agggtgtcgg
      301 cgtccttcac cgagaactgc gacaccgacg cgtgcgtctt cgcagggtcg tcgccgtaga
      361 tcgtgatcga tcgcccgtcg gggaacgcct ggtagtacgg cccgaacggc gtgacctggt
      421 agccgtgctt cgccagttgc agctcctcga tcaggaacgc cggcatcagc gacatcacgt
      481 acgagtaggt cgagaccttg atctcggggt gatcggcgaa cggcgcgctg gtatcgacgg
      541 cgccgcccac cttcggccgt gcctccagca ccacggtgcg ggcgccggac ttcgcgaagt
      601 aggcgccggc caccaggccg ttgtgcccac cgcctacgac gatcgcgtga taggacccgg
      661 tactcatgag aggccgtgca gcttccgcac gttgtcggcg agcacgccgc gaccgatcga
      721 ctcggcatcg ggcaccgaca ggaagtcccg gtcgacgaac gcgcccagga cccgctcgag
      781 cgactggcgc accagccggg ccgacaccgg gaacatctcg ggttcggagg actcgtcgca
      841 tccgtgcacc accttggcag ccggcaccgc ccccaggatc atctccagcg accagtcgat
      901 ctgcccccaa ccccacggga ccagctcgga cacgtccatg tagacgttcg gaagcaccgt
      961 ggggatgtac gtcgcctcgg ccacccacgg ccatcccgag tggatcatca ggatcggctg
     1021 ctcctggtgc tcgacgaaga acgagaacgc gtccgacggc ttagcatgcg tgaggttcac
     1081 gtcggggtcg cccgcgccga cgtgcacgtg gaacacgcgg tcgcgctcct tcgcgatgtc
     1141 gaacgcgcga cgcagcagga agtcccgtac cggcttggcg tgttcccggg attcgcgcca
     1201 gtcgtcggct cgccatcgct cgaacgccgc gacgcagtcc gaggtcgacg gatcggtgat
     1261 gtccaggccg gtgcggtagg cgatgatcgt cttgaacccg atcaaccgtg gatcgtccgc
     1321 ggcgccgttc gccaggtcct cgaaccgctg cacgaggccg tcgaacgttc cctcctcgcg
     1381 agcctcgacg atccagggct cgatccggcc gacgcgatgc accggcacgc cgccgagcgc
     1441 cgactcgaac tcatcccgcg tgatcgtggg ttgcgggtag ccctcgtcgg ccaccaccgc
     1501 gacgatgtgt tcggactcca gcagtccccg gacgtagccg ggcgggtcgg cgcgcagggc
     1561 ggcgtcgcgg gccgcgacca ccgcgtcctt cgtgggctcg cacccgaggt gctcggcgat
     1621 ccaccggcgc agcgccagac cgaacatcgt cgtctcggtg agctcctcca cgaacgcggc
     1681 cgcctccagg ttcgcgtggt tcgacgagat cagcgctgtc cccaggaaca tgcaccgcgt
     1741 ctcgaacgtg cgcggctcgc ggtcgagcag gtcctgggtg cggaacgggt ggcagtgcgc
     1801 gtcgatgacg tcggcgcccg tgagatcgat ctgcatcgct cgtcctcctg ggaccgcggc
     1861 cccgctcagt accgccatcg cagacggcgg atcatctcgt cgtcgtccag gccttcgtac
     1921 tgcgcgcatt cgccacgccg cgtggcgacg aaggtctcga acaggaagtc gcccatcgcc
     1981 gctcgaagca cgtcggaggc ggcgagccgt tccgccgcct ctgccaatga ggccggcagc
     2041 tgatggatgc cccgcgccac cttcac
//
LOCUS       DATDBZ010000060         3007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2271585, whole genome shotgun sequence.
ACCESSION   DATDBZ010000060 DATDBZ010000000
VERSION     DATDBZ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271585"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VIX62_01515"
     CDS             <1..442
                     /locus_tag="VIX62_01515"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEY5858947.1"
                     /translation="GTEDARFALDVDLAATFADAANVTPGVPQEGTSLLPLLDGSATS
                     WRTSFLVEHADPPATSGDSRYVPTYCAIRTTTHVFAVLTTGEEELYDLVADPFELQNL
                     LRPPGGQVDPANQALRNTLWSRLFTGLNGKPPMCSPLPPDYPAP"
     gene            complement(456..884)
                     /gene="arfB"
                     /locus_tag="VIX62_01520"
     CDS             complement(456..884)
                     /gene="arfB"
                     /locus_tag="VIX62_01520"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY5858948.1"
                     /translation="MVGEPLRVTRSVVVPDKELRWRFSRSSGPGGQSVNTADSRVELS
                     LDVSTTTALGPVLRPRAMERLQDRLVDGVLTVTASEHRSQFRNREAARERMASILADA
                     VAPPPKQRRPTKPSRGAVERRIAEKKRRAQTKRLRRSDDD"
     gene            957..1232
                     /locus_tag="VIX62_01525"
     CDS             957..1232
                     /locus_tag="VIX62_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858949.1"
                     /translation="MADYVECPCRGYRECPMCHDLRPCGEFGKSRWCPPCVRLRREGM
                     RPRAKRRGVMPAGRVCAGQGCETKLSVYNAGDRCSLCVNKDLVIRVP"
     gene            1235..2107
                     /locus_tag="VIX62_01530"
     CDS             1235..2107
                     /locus_tag="VIX62_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5858950.1"
                     /translation="MAFDTLQVGDIEVLALIDGDQDLGSPIAEAYPDIPIERLMAHRD
                     LEPGIHGPDGSRRLRIRAWLVRHPAGVLLVDTGIGRSGAPGVEWFGVPGTLLDALRET
                     GTAPDAIDTVLLTHVHDDHIGGTVAFDDHQVPAPAFPNARYVLQAADREWQRELARTD
                     EEDRAIETLLLRPLEAAGVLDVIEGDQRLAEGLEAHLAPGHTPGHQVVRIHSEDRRAI
                     ISADAFNHPLQVGHPDWPSGSDDVPADAAMTRRALLAEALSHPGTTIAPTHFTDSFGH
                     IRSGADGVAVWTAS"
     gene            2265..2723
                     /locus_tag="VIX62_01535"
     CDS             2265..2723
                     /locus_tag="VIX62_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY5858951.1"
                     /translation="MRNLSRAIIVLTAFAVVVSSGPVASAGPTSPKARALRSTMFQLV
                     NGSRRHHDLRALDLNATLSKKAWRHSKRMAERNSVYHTTDLWSLVRRYRPSAWGENVG
                     MAGTLKRMEVLFMRSAPHRANILSSRFRHIGVGVVRARDRIWVTLDFYGG"
     gene            complement(2732..>3007)
                     /locus_tag="VIX62_01540"
     CDS             complement(2732..>3007)
                     /locus_tag="VIX62_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5858952.1"
                     /translation="TGLTQSQLAARAATSQSAISSMETGNRLPSVRTLMRIAEAAGFE
                     LVVGLRVPEAHNPTALGALIVDPEDDLADFVPITTPTALDRLPATTI"
BASE COUNT          458 a         1068 c         1009 g          472 t
ORIGIN      
        1 ggggaccgag gatgcacggt tcgccttgga cgtcgatctc gcggccacgt tcgccgacgc
       61 cgcgaacgtg acgcccggcg tcccgcagga gggcactagc ctgctgccgt tgttggacgg
      121 ctcggccacg tcgtggagga cgagcttcct ggtggagcac gccgatcccc ccgccacctc
      181 cggtgactcc cgctacgtgc cgacctattg cgccatcagg accaccaccc acgtcttcgc
      241 ggtgctgacc accggtgagg aggagctcta tgacctcgtg gccgatccct tcgaacttca
      301 gaacctgctg cgcccaccgg gaggtcaggt cgacccggcg aaccaggccc tgcggaacac
      361 gctctggagc cggctgttca ccgggctgaa cggcaaaccg ccgatgtgct ccccgctgcc
      421 gccggattac ccggcgccct gacccgctcc tgagctcaat cgtcgtccga gcgccggagg
      481 cgtttcgtct gcgctcggcg cttcttctcg gcgatccgac gttcgaccgc cccccggctc
      541 ggcttcgtgg gacggcgctg cttcggtggc ggggccaccg cgtcggcgag gatcgacgcc
      601 atccgttccc gcgccgcctc acgattgcga aactgcgagc gatgttcgga tgccgtcacg
      661 gtgagcaccc cgtcgaccag ccggtcctgc agccgctcca tggcacgggg gcgcagcacg
      721 ggtccaaggg ccgtcgtggt cgacacgtcc aggctgagct cgacgcgcga gtccgcggtg
      781 ttgaccgact gcccgcccgg tcccgacgaa cggctgaacc gccaccgcag ctccttgtcg
      841 ggaacgacga ccgagcgcgt gacgcggagc ggctctccca ccatcgcatc agtatgcggg
      901 cccacgacct gcggaaccgc gatgatgatc gcggtccgag gcgtaccctg gaagggatgg
      961 ccgactacgt cgaatgcccc tgccgaggct atcgggagtg cccgatgtgc cacgacctgc
     1021 gcccgtgcgg cgagttcggc aagagccgtt ggtgcccccc gtgcgttcgc ctgcgccgcg
     1081 aaggcatgcg accccgcgcg aagcggcgcg gcgtgatgcc ggcgggaagg gtctgcgccg
     1141 ggcagggatg cgagaccaag ctcagcgtct acaacgccgg tgaccgctgt tcgctgtgcg
     1201 tgaacaagga cctcgtcatc cgggtcccgt agcaatggcg ttcgatacgt tgcaggtcgg
     1261 cgacatcgag gtcctcgccc tgatcgacgg cgaccaggat ctgggttcgc cgatcgccga
     1321 ggcttacccc gacatcccca tcgagcgcct gatggcccac cgcgacctcg aaccggggat
     1381 ccacggcccc gatgggtccc gccgattgcg gatccgcgcc tggctggtgc gccacccggc
     1441 cggtgtcctg ctcgtcgaca cggggatcgg gcggagcggt gcccctgggg tcgagtggtt
     1501 cggcgtgccc gggacgttgc tcgatgccct ccgcgagacc gggacggcac cggacgcgat
     1561 cgacaccgtg ctcctgaccc atgtgcacga cgatcacatc ggcggaaccg tggcgttcga
     1621 cgaccaccag gtccccgcgc cggccttccc gaacgcccgg tacgtcctgc aggcggccga
     1681 ccgcgagtgg cagcgggagc tcgcccgcac cgatgaggag gaccgcgcga tcgagacgct
     1741 gctgcttcgg ccgttggaag cggcgggggt cctggacgtg atcgagggcg atcagcgtct
     1801 ggcggagggg ctcgaggcgc acctcgcccc cggtcatacg cccggtcatc aggtggtcag
     1861 gatccactcg gaggaccggc gcgcgatcat ctcggccgac gcgttcaacc atccgctcca
     1921 ggtgggccac cccgactggc cgtcgggcag cgacgacgtc ccggcggacg ccgccatgac
     1981 ccgtcgggcc ctgctggccg aggcgctgtc gcatccagga accacgatcg ccccgaccca
     2041 cttcaccgac tcgttcggcc acatccgttc gggtgcagat ggtgtagcgg tatggaccgc
     2101 ctcttagtgg cgcggagggg ccgaccgatc cggtccttag cctagattta acggtcggcg
     2161 aggccctctg ccccccagat gacctcacga aaccgtttcc tcatgggcct acggcagatg
     2221 cgtgacgcct cagggatggc tcgctagggt cgatacccat acatatgcgg aatctctcac
     2281 gcgccatcat cgtcctcacc gcgttcgcgg tggtcgtctc ctcggggccc gtagcgtcag
     2341 ccggacccac cagccccaag gctcgggccc tgcggtccac gatgttccag ctggtcaacg
     2401 gatcgcgtcg ccatcacgac cttcgggccc tcgatctgaa cgcgacgctg tccaagaagg
     2461 cctggcggca ctcgaagcgc atggccgagc gcaacagcgt ctaccacacg accgacctct
     2521 ggtccctcgt ccggcgctac cggccgtcgg cctggggcga gaacgtgggc atggccggga
     2581 cgctgaagcg catggaggtc ctgttcatgc ggagcgcgcc ccaccgcgcc aacatcctga
     2641 gcagccgctt ccgacacatc ggcgtgggag tggtgcgcgc tcgggatcgc atctgggtga
     2701 cgctggactt ctacggcgga taggccggcg gctagatcgt cgttgccggc aggcggtcca
     2761 acgccgtcgg cgtcgtgatc ggcacgaaat cggccagatc gtcctccgga tccacgatca
     2821 gagcgccgag cgcggtcggg ttgtgagctt ctggcacacg aagcccgacc acgagctcga
     2881 accctgcggc ctcggcgatc ctcatcaacg tgcgaaccga tgggagccga ttcccggtct
     2941 ccatcgagga gatcgccgac tgcgaggtgg cggcgcgcgc cgcgagttgc gactgcgtga
     3001 gcccggt
//
LOCUS       DATDBZ010000061         3779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_839356, whole genome shotgun sequence.
ACCESSION   DATDBZ010000061 DATDBZ010000000
VERSION     DATDBZ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839356"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="VIX62_01545"
     CDS             complement(<1..1491)
                     /locus_tag="VIX62_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY5858953.1"
                     /translation="MSTEDFFSTDIEGLPAAADPVVVRLRDGDTHQMRLHSVRKQIGP
                     DELRMLTYNGSIPGPTLHVDEGSEITVEVVNDTDMETTVHWHGLRLENRHDGVPHETQ
                     EPIPIGGTYTHELRFPDAGLYWYHPHMREDYAQEMGLYGPIVVEPSDPDYWPPADRFL
                     TLTLDDLLVEDGQIAAFHKSGPTFVAMGRFGNVLLVNGNTEFAADVTAGEVVRLYLVN
                     TANTRIFNVAIADARMKLVGGDSGRVEREEFVEELLLAPSERAVVDVHFDRPGVATLQ
                     HRTPDTTYELGGFAVSGGPTGQSSRSFDELREASELTAERGRIQADLDRPPDKILAFY
                     AEMPLLYGDDAADAAAGYVCPMHPEVTSDEPGTCPKCGMKLIPAAPASYVCPMHPEVT
                     SDEPGTCPQCGMKLLPGDPDGPPNGAHEHEHGHDHGDGLEWEDLMPEINRESTPSNML
                     WKLIDRETGAENAAIAWDFTVGDRVKIRLVNEMESDHPMHHPFHFHG"
     gene            complement(1498..1854)
                     /locus_tag="VIX62_01550"
     CDS             complement(1498..1854)
                     /locus_tag="VIX62_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3703 domain-containing protein"
                     /protein_id="HEY5858954.1"
                     /translation="MKQDRERLLQVWANERQLAKDAAAQGDSAKAWAHLERAHIVSQP
                     LVLAHVRTHVAMLGYAVSHRDGHEIAGQLFRLVVAGPGSLTGRYPVGNTGGANVSAFL
                     VMDIPEDLRPLLPASS"
     gene            complement(1906..2382)
                     /locus_tag="VIX62_01555"
     CDS             complement(1906..2382)
                     /locus_tag="VIX62_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858955.1"
                     /translation="MTEILTRPDEDLLLVNPPAGDRRMSARTPILWGLVFGAVQAASP
                     LAFFWIDPATFHAISIALIAAIYIGFAVADGRAGVIAVESTVALAFTVIAAAAVTGSA
                     WLLVVGYAAHGTKDFWQERTHFVATTRWWPPFCAAVDFLVAAILVLEILAGVDFRY"
     gene            complement(2440..2910)
                     /locus_tag="VIX62_01560"
     CDS             complement(2440..2910)
                     /locus_tag="VIX62_01560"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5858956.1"
                     /translation="MTGPGVSVIVNAPPSIVYDHISDVERMPEWSPEVVRCRWLGNAS
                     TAEPGARFRGWSRKGWHRWSTVSTVVIADRPTAFAFQVQFLGLAVATWSYRCESTGTG
                     STAVTEAVVDERGLILRRVSPLITGSSDRGARNDETMHVTLERVCAAAEAAARH"
     gene            complement(2907..>3779)
                     /locus_tag="VIX62_01565"
     CDS             complement(2907..>3779)
                     /locus_tag="VIX62_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5858957.1"
                     /translation="LRRAATIGGVPPAIRAKVLGGYALLAAGLGNLDEALTAARSGTD
                     QARLAGDDTAIGFGLNATAVALWALGDLDAAAVAHDEAIERYVAAGDAWGEAVCRVLR
                     ARTALDAHDPQGEDRLRDALLAARRCGDAHVEGIALGLLAEAEGRQGRTDAAVATAAE
                     SLRLHESIGYTEGTVASLRVLARLHRDAGEPAAAHEVLLRSLRLAWKMQHLAAICESL
                     EELARLAMDSGDPERARVLLVVAEAERLRHDLPLRSQDQPQIDELRAALEEGEGRFRL
                     PVPELSETVSTVLR"
BASE COUNT          594 a         1282 c         1287 g          616 t
ORIGIN      
        1 gccgtggaag tggaacggat ggtgcatcgg gtggtccgac tccatctcgt tcaccagccg
       61 gatcttgacc cggtcgccca ccgtgaagtc ccaggcgatg gctgcgttct ccgcgccggt
      121 ttctcggtcg atcagcttcc agagcatgtt cgagggagtc gactctcggt tgatctccgg
      181 catgaggtcc tcccactcca gcccgtcgcc gtggtcgtgc ccgtgctcgt gctcgtgcgc
      241 gccgttgggg ggcccatctg ggtccccggg aagcagcttc atcccgcact gcggacaggt
      301 ccccggttcg tcgctggtga cctccgggtg catcggacac acgtaggagg ctggtgctgc
      361 gggaatcagc ttcatcccgc acttcgggca cgtgcccggc tcgtcgctgg tgacctccgg
      421 gtgcatcgga cacacgtagc ccgccgccgc atccgctgcg tcgtcgccat agagcaatgg
      481 catctcggcg tagaaggcca ggatcttgtc cggcggacgg tccaggtccg cctgaatccg
      541 cccgcgctcc gccgtcagtt cggaagcttc gcggagctcg tcgaatgacc gactcgactg
      601 tccggtcggg cctcctgaca cggcaaagcc gccgagctca taggtcgtgt cgggggtccg
      661 gtgctgcagc gtcgccactc cgggcctgtc gaagtggaca tcgacgacag ctcgctcgga
      721 aggcgccagg agcagctcct ccacgaactc ttcgcgctcc acgcggccgg agtcgcctcc
      781 gaccaacttc atccgtgcgt cggcgatggc gacgttgaag atccgggtgt tcgcggtgtt
      841 gacgaggtag agccggacca cctccccagc ggtcacatcg gcggcgaact cggtgttccc
      901 gttcacgagc agcacattgc cgaatcggcc catggccacg aacgtcggac cggacttgtg
      961 gaaggcggcg atctgaccgt cctcgacgag gaggtcgtcg agagtcagcg tgaggaagcg
     1021 gtccgctggc ggccagtagt ccgggtcgga cggctcgacc acgatcggac cgtagaggcc
     1081 catctcctgg gcgtagtcct cgcgcatgtg cgggtggtac cagtagaggc cggcgtcggg
     1141 gaaccggagc tcgtgggtgt aggtcccacc gatcgggatg ggttcctggg tctcgtgcgg
     1201 cacgccgtcg tggcggttct cgagccggag tccgtgccag tggacggttg tctccatgtc
     1261 ggtgtcgttg acgacctcga cggtgatctc agagccctca tcgacgtgga gggtcggtcc
     1321 gggaatcgag ccgttgtagg tcagcatgcg cagctcgtcg gggccgatct gctttcgcac
     1381 cgagtgcagg cgcatctggt gggtgtcgcc gtcgcggagc ctgacgacga ccggatcagc
     1441 ggccgccggg agaccctcga tgtccgttga gaagaagtcc tcggtactca tggcgattca
     1501 ggagctcgcc ggcagcagcg ggcgcaggtc ctccgggatg tccatgacca ggaacgcgct
     1561 gacgttggct cccccggtgt tgccaacggg atagcggccc gtcaacgagc ccggtccggc
     1621 caccacgaga cggaagagct gtcccgcgat ctcatgacca tcgcggtggc tcaccgcgta
     1681 cccgagcatg gccacgtgcg tgcgcacgtg cgccagcacc aacggttggc tcacgatgtg
     1741 ggcgcgctcc aggtgggccc acgccttcgc gctgtcacct tgcgccgccg catccttggc
     1801 caactgacgt tcgttcgccc acacctgcaa cagacgttcg cggtcttgct tcatgtggtc
     1861 tccattcatc tcagggcgcc gaggcacggt tcgagtgccc ggggctcagt aacggaagtc
     1921 aacgccggcc aggatctcga ggacgaggat cgcagcgacc aggaagtcga ctgcggcgca
     1981 gaagggtggc caccagcggg tcgtggcgac gaagtgcgtg cgttcctgcc agaagtcttt
     2041 ggttccgtgg gccgcatacc ccacgacgag caaccaggcg gatccggtga ccgcggccgc
     2101 ggcgatcacg gtgaacgcga gcgcgacggt cgattcgacg gcgatgacac cggcccgccc
     2161 atcggccacg gcgaatccga tgtagatcgc cgcgatcaag gcgatcgaaa tcgcgtggaa
     2221 cgtcgccgga tcgatccaga agaaggcgag gggcgaagcc gcttggaccg cgccaaagac
     2281 aagaccccag agaatcggcg tgcgcgcgct catccggcga tcgcccgcag gcgggttgac
     2341 aagcagcagg tcttcgtccg gcctggtgag gatttcggtc atgccgtgac tctccgcctc
     2401 gggtctgtca tcgcgctttc acacgggcaa aaccaagcgt cagtggcgtg ccgccgcctc
     2461 ggccgccgca cagactcgtt cgagcgtcac gtgcatcgtc tcgtcgttgc gggcacctcg
     2521 gtcggacgaa ccggtgatga gcggcgagac gcggcggagg atcagtcccc gttcgtccac
     2581 gacggcttcg gtcaccgccg tacttccggt gcccgtggat tcgcagcggt acgaccacgt
     2641 ggccaccgcg aggccgagga actgcacctg gaacgcgaac gccgtcggtc gatcggcgat
     2701 gaccacggtc gagaccgtcg accaccggtg ccatcccttg cggctccatc ctcgaaaccg
     2761 ggctcctggc tcggcggtcg aggcgttacc gagccatcgg caacggacga cctcggggct
     2821 ccactcgggc atgcgctcga catcgctgat gtggtcgtag acgatcgagg gaggggcgtt
     2881 gacgatgacc gacacccccg gcccggtcat cggagcacgg tgctcaccgt ctcggacagc
     2941 tccgggaccg ggagccggaa gcgtccttca ccctcctcca acgcggcgcg gagctcgtcg
     3001 atttgcggct gatcctgcga ccggagcggc agatcgtgac gcaggcgctc ggcctccgca
     3061 acgacgagga ggacgcgggc gcgctcggga tcacccgagt ccatcgccaa ccgcgcaagc
     3121 tcctcgaggg actcacagat cgccgcgagg tgctgcatct tccatgccag ccgcaggctc
     3181 cgcagaagca cctcgtgggc cgctgcgggc tcgcccgcat cccggtgcaa ccgcgccaac
     3241 acccgcagtg aggcgactgt tccctccgtg tatccgatgg actcgtggag ccggaggctt
     3301 tcggctgcgg tagcgacggc ggcgtcggtc cggccctgcc ggccctcggc ctcggcgagt
     3361 agccccaagg cgatcccctc cacgtgggca tcgccgcagc ggcgagccgc caggagcgca
     3421 tcacgcaacc ggtcctcgcc ctgcggatcg tgcgcgtcga gcgccgtccg cgcccgcaag
     3481 acgcgacaca cggcctcgcc ccaggcgtct cccgcggcga cgtagcgctc gatcgcctcg
     3541 tcgtgagcca ccgcggcggc atccaggtcc ccgagcgccc acagggcgac ggcagttgcg
     3601 ttgagaccga atccgatcgc ggtgtcatcg ccggctaaac gtgcctgatc ggtgccggac
     3661 cgcgccgctg tcagagcctc gtcgaggttg ccgaggcccg cagccaggag tgcgtacccg
     3721 ccgagcacct tggcgcggat ggccgggggg acgccgccga tggtcgccgc ccggcggag
//
LOCUS       DATDBZ010000062         2457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2680216, whole genome shotgun sequence.
ACCESSION   DATDBZ010000062 DATDBZ010000000
VERSION     DATDBZ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2680216"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..1923
                     /locus_tag="VIX62_01570"
     CDS             478..1923
                     /locus_tag="VIX62_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858958.1"
                     /translation="MRALAHGLDEAKVPYCHFKSNEAIGKSLRGENDLDLLIGFDAWG
                     RFLSVLSRLGFKEGVPHRVRRIPAVAQFYGLDTATGRLVQVHAHRRLVFGDDMTKNFV
                     LDGEDRYIAAATRDLGPLPVASPEFELLLFAFRMVAKHCTPDAIAMLQGRLSASERRE
                     LIWLIERADLDHVASIVGSDLPFVGRDLFMRCIEAIGPTAGPWRKVRTAGALHRAFAA
                     HRRRSRPVDAFLRVERRVGRVARRIVRGKSRKRPVAGGALIAFVGGDGAGKSTAVADV
                     TGWLGGTFDVRRIHLGKPPPSLVTVAVKGPMYVLRTLGMFRSTLVPSHVLREDHGSHP
                     GLPWIVWHVLTARDRLRLYRDATASVLRGTIVISDRFPLPEVQTMDAAKTAWLAEHPR
                     SGRLARWLIELERRSYAAFMPPDVLVVLRVDPAVAVERKRHEEEPGFVRPRSEEIWRA
                     DWSGTSATVVDSGHDLEEVRRTLRSLVWAGL"
     gene            2142..>2457
                     /locus_tag="VIX62_01575"
     CDS             2142..>2457
                     /locus_tag="VIX62_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858959.1"
                     /translation="MAHHILRSHQPGPAGVASRWIQWSTTQALIDRARSRSGVHLFDE
                     GVLQALWSIALHGDAAAILRALRGSTGLWASPDLVLVLDTPVDELVQRLRLRRSRHSR
                     VQR"
BASE COUNT          338 a          849 c          882 g          388 t
ORIGIN      
        1 agggcgtgcg ggggcgcaac agcgacaacg acctctgcaa ggcgacgctc ggctgggcgc
       61 ccgaggtcac gctcgaggat ggcctcaagt ccacgtacgc ctggatcgag gatcagctcg
      121 aggcggctgg gcgattgccg atgtctgcga gcgcgtaagc accgagttgc accgcaaagg
      181 ccgaggggcc cccgcacggg ggcccctcgt gcgttcgcca acgaggctca gtgttgcttg
      241 gccgccggag cgtccgggac ctgtcggcac actcgtccgt tcgtcgtagc cgagctacca
      301 catcgggcgg cggcgaaggt gccgacgacg gcctagcgtt cctccggtcc gcgtgaccgc
      361 gcgggtccac ccgatgagag gtgtcgcttg gtctcgacag cccatcacag aggagcgccc
      421 gcgacgtccg tatcggcgga accggccgcg acgcaggatg ggcagccgct cgaactcgtg
      481 cgggcgctgg cccatggtct cgacgaggcc aaggtccctt actgccactt caagagcaac
      541 gaggcgatcg ggaagtcgct ccgaggcgag aacgatctgg acctgctgat cggattcgat
      601 gcgtggggcc gattcctctc ggtcctgtcg cgactgggct tcaaggaagg cgtgccgcac
      661 cgggtccggc gtatccccgc ggtcgcacag ttctacgggc tggatacggc gaccggacga
      721 ctcgtgcagg tgcacgcgca ccggcggctc gtcttcggcg acgacatgac gaagaacttc
      781 gtgctcgacg gcgaggaccg atacatcgcg gccgccacga gggatctcgg cccgcttccc
      841 gtggcgtcgc ccgagttcga gctcctgctc ttcgcgttcc gcatggtcgc gaagcactgc
      901 acgcccgatg ccatcgcgat gctccagggt cgattgtcgg cgtcggagcg ccgcgagctg
      961 atctggctca tcgagcgcgc cgatctcgat cacgtcgcct cgatcgtcgg atccgacctc
     1021 ccgttcgtgg gccgggacct gttcatgaga tgcatcgagg cgatcggccc caccgccggc
     1081 ccgtggcgaa aggtccgcac tgccggtgcg ctccatcgcg ccttcgcggc ccaccgcagg
     1141 cgttctcggc cggtcgacgc gttcctgcgc gtcgaacgca gggtgggcag ggtcgcgcga
     1201 cgcatcgtcc ggggcaagtc acgcaagcga cccgtcgccg gtggtgcgct gatcgccttc
     1261 gtgggcggtg acggcgccgg caagtccacc gcggtcgccg acgtcacggg atggctgggt
     1321 ggaacgttcg acgtccggcg catccacctc ggcaagccgc cgccgtccct cgtgacggtc
     1381 gccgtgaagg ggccgatgta cgtgctgcgt accctgggga tgttcaggtc cacgctggtg
     1441 ccctcgcacg tcctgcgcga ggaccacgga tcgcacccgg gcctgccctg gatcgtgtgg
     1501 cacgtgctca cggcgcgcga tcgcctacgc ctctaccgcg atgccacggc atccgtgctg
     1561 cggggcacga tcgtcatctc cgaccgcttc ccgctgcccg aggtgcagac gatggatgcc
     1621 gccaagacgg cctggctggc cgagcacccg cggtccggcc gcctggcgcg gtggctcatc
     1681 gagctagaac gacgttccta cgcggcgttc atgcctcccg acgtcctcgt cgtgctgcgg
     1741 gtcgacccgg ccgttgcggt cgagcgcaag cgccacgagg aggagcccgg cttcgtgcgt
     1801 ccccggtccg aggagatctg gcgcgcggac tggagcggga cctcggccac ggtggtggat
     1861 tcgggccacg acctggagga ggtccggcgg accctccgat ccctcgtgtg ggcaggactg
     1921 tgacgtcgcc gataccgcgt ggggtggtcg tggagctgtg cggactgccg ggggcgggga
     1981 agacctcgct ggtccgcacg atcggcaccg atccaaccgc accgcgcgtg acgcggccga
     2041 ccggacgcgt cgcgccggag gtcgcacccg ctcggcgtgt gccacggaag ttgcgactgg
     2101 ccctggaggg ctccgtcagg cggccggggt ccgagctgtc gatggcgcac cacatccttc
     2161 gttcgcatca gccggggccg gcgggggtgg cgtcgcggtg gatccagtgg tccacgacgc
     2221 aggccttgat cgaccgggcg aggagccgga gcggcgtcca cctgttcgac gagggcgtgc
     2281 tccaggcgct ctggtcgatc gccctgcatg gcgacgcggc cgccatcctg cgcgcacttc
     2341 gcggctcgac cggcctgtgg gcctcgcccg atctcgtgct cgtgctcgac accccggtcg
     2401 acgagctggt gcagcgtctc cggctccggc gctcgcggca cagtcgggtg cagcgac
//
LOCUS       DATDBZ010000063         2227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_13074,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000063 DATDBZ010000000
VERSION     DATDBZ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13074"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..833
                     /locus_tag="VIX62_01580"
     CDS             42..833
                     /locus_tag="VIX62_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5858960.1"
                     /translation="MLTISQLASYAGVTVRTVRHYHQIGLLAEPERDHSGYRSYDTAA
                     VVRLVRIRVLADAGVPLARVQELLVASPDEFARAVAEIDKALSADIRRLQSNRKRIAK
                     LAAGDHLALPQIVLDYLDRLRWLGVGEAYIELERDAWIMLAAQDPLSIDAVIAEKNRQ
                     LDEDPDMTRLYRLLGSAPEWPPDDPRVVELADLVEHLRIRAVQSGELEIGDADTSDPL
                     TALLDAAMTESAPVARQLLAILEQRGWRGWNRLERIPEGRISRDR"
     gene            1008..1892
                     /locus_tag="VIX62_01585"
     CDS             1008..1892
                     /locus_tag="VIX62_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858961.1"
                     /translation="MGAPSHRRAGVRGTPRIAAVLGATLFTVACLAACTVTPESAAPT
                     ATPLAEPFVPFASPSGTELPEPSVPTATEAPYLVPAFDGAEPLPVEDGTTHVLKNLNQ
                     DASAFHTVEYTVPPGWETGGVYIGKKLGQPGEVAISFWTPSGVYSDPCQRTANLSPID
                     LAVHTHDGGGELILLGYPRIGLSAQDGRAATEPRSLIVDDPSEAGGTIALRLELTVPA
                     DLDPASCDDGVYVAWPGTSAGDRPNDNHVAGQTDIIYLVDVDHGPLVIDASFRPESSP
                     EDIEELYSVLGSIVMDRY"
     gene            complement(1917..>2227)
                     /locus_tag="VIX62_01590"
     CDS             complement(1917..>2227)
                     /locus_tag="VIX62_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEY5858962.1"
                     /translation="SATAVADSKQTLICTYLSAAVLVGLVLNSLFGWWWADAIAGLVI
                     AGFAVREGIEAWRGDTCATSIGMLLEDEHDDHEHDTLARKPVVQRAAHAPRHPRAARQ
                     "
BASE COUNT          370 a          789 c          717 g          351 t
ORIGIN      
        1 gaggcggtcc gcggcgtcgc cggttgaggc agtctggagc catgctcacg atcagccaac
       61 tggcctccta tgccggggtg acggtgcgaa cagtcaggca ctatcaccag atcggcctgc
      121 tcgccgagcc cgaacgcgac cactccggct accgcagcta tgacacggcc gccgtggtgc
      181 ggctggtccg catccgcgtg cttgccgatg caggcgtgcc cctcgcccgc gtgcaggaac
      241 tcctcgtcgc cagccccgac gaattcgccc gcgcagtcgc ggagatcgac aaggccctga
      301 gcgccgacat ccgtcgcctg cagagcaacc gcaagcggat cgccaagctc gccgccggcg
      361 atcacctggc ccttccccag atcgtcttgg actacttgga ccggctccgc tggctcggcg
      421 tcggcgaggc ctacatcgag ctcgagcggg acgcctggat catgctcgcc gcgcaggatc
      481 cgctcagcat cgacgccgtg atcgccgaga agaaccgcca gctggacgag gacccggaca
      541 tgacgcggct ctatcgcctc cttggctccg cgccggaatg gccgccggac gatccccgag
      601 tcgtcgaact cgccgacctc gtcgagcacc tccgcatccg cgcagtccag agcggcgagc
      661 tggagatcgg cgacgccgat acgtcggacc ctcttactgc cctgctggac gcggccatga
      721 ccgagtcggc accggtcgct cgacagctgc tggcgatcct cgagcaacgc gggtggcgtg
      781 gctggaatcg actcgaacga atccccgaag ggcggatttc ccgcgatcgc tgagccggcc
      841 ccaccccccg aaggatcacg gtcaggtaca ccacccccgc ccgctgcggg atccgcctgc
      901 gggcgtgcga tgaccgaccg gtcggcggga gcgatctagc cgagggcgca gggtgccatc
      961 tccggcgagt tcttgcaggg tgtctcgcgg aggtggacaa tgcgaccatg ggcgcaccct
     1021 cgcaccgccg cgccggcgta cggggtacgc cgcggatcgc cgcagttctg ggcgcgaccc
     1081 tgttcaccgt ggcatgtctt gcagcgtgca cagtgacgcc tgagtccgcc gcaccgacgg
     1141 cgacgccact cgcagagccg ttcgttccgt tcgcgtcgcc gagcggcacg gaacttccag
     1201 aaccgtccgt acccaccgca accgaagcgc cgtacctcgt tccagccttc gatggcgcag
     1261 agccgctgcc cgtggaagat ggcacaacac acgttctgaa gaacctgaac caggacgctt
     1321 cggctttcca caccgtcgag tacacggttc cacccgggtg ggagaccggt ggcgtataca
     1381 tcggcaaaaa gctcggccaa cccggcgagg ttgcgatcag cttctggacg ccctccggcg
     1441 tctattcgga cccatgccag cgaaccgcga acctgagtcc gatcgacctt gccgtccaca
     1501 cccacgacgg tggcggcgag ctcatcctct tgggctatcc ccggatcggg ctttcggcgc
     1561 aggacggccg cgcggcgacc gaaccacgtt cgctgatcgt cgacgatcct tctgaggccg
     1621 gtggaaccat cgcgctccga ctcgagctga ccgtacccgc ggatctcgac cctgcctcct
     1681 gcgacgacgg cgtctacgtg gcgtggccgg gcaccagtgc aggtgatcgc ccgaacgaca
     1741 accatgtcgc ggggcagacg gacatcatct acctggtcga tgtcgaccat gggcctctcg
     1801 tcatcgacgc gtcgttccgg ccagaatcct cgcccgagga catcgaagag ctctactccg
     1861 tgctcggatc gatcgtcatg gaccggtact gactctggcg actcgcccga cgtcactcat
     1921 tggcgagcag cgcgcgggtg gcgcggtgca tgcgctgccc gctgaacgac cggcttgcgg
     1981 gcaagggtgt cgtgttcgtg gtcgtcgtgc tcgtcctcga ggagcatccc gatggaggtg
     2041 gcgcaagtgt cgcctcgcca cgcttcgatc ccctcgcgta cggcgaaacc ggcgatgacg
     2101 aggcctgcga ttgcatcggc ccaccaccac ccgaacaggc tgttcaggac gaggccgacc
     2161 agcacggccg cggagaggta ggtgcagatg agggtctgct tcgagtcggc gacggcggtc
     2221 gccgagc
//
LOCUS       DATDBZ010000064         9790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_840079, whole genome shotgun sequence.
ACCESSION   DATDBZ010000064 DATDBZ010000000
VERSION     DATDBZ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840079"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VIX62_01595"
     CDS             complement(<1..472)
                     /locus_tag="VIX62_01595"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY5858963.1"
                     /translation="MELQVRPDRETFDALAAEWPLVPVWAELLSDVSTPVGLFPALTG
                     DGPGVLFESVERSERWGRYSFVAGDPAATIVAGPDGIEVTERARTIPIDVPDGGPVRE
                     GLIRIARSLRAPRLPGLPPLTGGLMGYFAFEAAELLDGHPVPDPASAPVPPIGLL"
     gene            776..1942
                     /locus_tag="VIX62_01600"
     CDS             776..1942
                     /locus_tag="VIX62_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 repeat- and reductase domain-containing
                     protein"
                     /protein_id="HEY5858964.1"
                     /translation="MVPAPRSARRPRSTRRRALTIAPIAAALVAVLAQPALAAGSISS
                     WGGNSYGQLGDGTFTGHGSATAIASLTSMVSLHGGRDHVAALKSDGTVWTWGRNQYGQ
                     IGDGTGTSRPNPTLVTGLTGVIAVETGHYNTMALKSDGTVWMWGWNKYGTNGDGTTTK
                     RTRPVLVKNLTNVVAIAGGRDQMLAVKSDGTVWAWGYNAFGNLGDGTFTTRTTPVQVS
                     GLAGAIDVAGGRDHSLVLKSDGTVWCFGYNVHGECGVGNTSNKNLPVQVSGLTGVTAI
                     TAGANHSVALKSDGTVWTWGQGSNGRLGNNSTANRLTPVQVSNLTGVVAIGTGRMHTL
                     AAKSDGTVWAWGFNATGQLGNGNTTNQKVPVLVQGLTGATMFGGGRDHSVALVP"
     gene            1996..3702
                     /locus_tag="VIX62_01605"
     CDS             1996..3702
                     /locus_tag="VIX62_01605"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY5858965.1"
                     /translation="MRLSRSFTPTLREDPAEAEIASHRLLLRGAFVRQVAAGIYTFLP
                     MGLRTMRRIEAIIREEMEDSGAIEIRMPIAIPAEPWKATGRWDLYGDQLFRLQDRHGR
                     DMLLGPTQEEVVAPLVAGEVSSYRDLPLNLYQVEWKYRDEFRPRFGLLRGREFLMKDA
                     YSFDRDEPAMAASYEVMKAAYHRIFDRCGLDYAVVEAEAGQIGGGTNHEFMARAEVGE
                     DLFVECEHGDYIADLKAARPMAPDASAPATQELTVVDTPDTPTIETLAALLDIDASQT
                     LKCVLFDVGGRTVAVLVPGDREVNGAKLEQLVFPATVRMFEDEDFARHGFAKGYVGPQ
                     GFDEDVTIYADHTVRGGHDWVTGSNQVDRHVTGANAGRDFRVDAYEDLVVLREGDACP
                     VDGGQLHIGRSIVVGHIYQLGKRYSEPLEATFTEEDGSEQVFWMGCYGIGISRILAAA
                     AEQCHDEDGLTLPKVLAPFEVVVILANHDDEQAIAEAERIYHDLTSAGVGVALDDRDE
                     RAGVKFADADLIGYPVQVTVGKRGIANGTVDLKLRSTGERSNAPLAEAAQAAVALLAT
                     AP"
     gene            3821..4303
                     /gene="rimP"
                     /locus_tag="VIX62_01610"
     CDS             3821..4303
                     /gene="rimP"
                     /locus_tag="VIX62_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY5858966.1"
                     /translation="MDAEALVRPVIEQAGFEFVELATTREHGRKVLRVVVDRPGGVDL
                     DALSQLSATVSRHLDQEGYEDGPYGLEVSSPGIERPLKRPEHFARSVGEQVKVKTTAP
                     MAGSRTHTGTLVSADEDAIVIATADGDEGTGDLRVPYADIVSARTVVDWDAELKRSNA
                     "
     gene            4300..5550
                     /gene="nusA"
                     /locus_tag="VIX62_01615"
     CDS             4300..5550
                     /gene="nusA"
                     /locus_tag="VIX62_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY5858967.1"
                     /translation="MNAEMIAALRELEREKGIAFETILTGLAEAMASAYKTWWKQENP
                     DADDDFVGFRAELDPETGDLRMWKQELEEIEVPAAEEGGDPTLEMKVLGEAEVEVTDD
                     FKGRIGAQTAKQVIFQKLRDAEREMTYEEFAGREGDVVTGIVQQQERRYTLLDLGKVE
                     ALLPQAEQVPSEPYRHGERLKAFITEVRKGTKGPQIVVSRSHPGLLKALFAMEVPEIE
                     EGIVEIRGVAREPGHRSKIAVISNEPGVDPVGACVGPKGSRVRMVVNELRGEKIDVVP
                     WSDNPGELVANALQPAKVKEVRTDEATGTAMVIVPDYQLSLAIGKEGQNARLAARLTG
                     WRIDIKSETQVADEAKAPAAAPVAPPAEEVAEPATDAEAELEVEVAEEVVADEAVEEL
                     AAEEAAVEAIDEVVAEEAEAPEGV"
     gene            5552..5812
                     /locus_tag="VIX62_01620"
     CDS             5552..5812
                     /locus_tag="VIX62_01620"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEY5858968.1"
                     /translation="MAPTRTCVGCREPSSKSGLLRIVRSAEAGVQLDPDGTAPGRGAY
                     VHRRAACLEAAGAEGVLARALRTGLGPDELGRLRDQMRTGVL"
     gene            5809..8010
                     /gene="infB"
                     /locus_tag="VIX62_01625"
     CDS             5809..8010
                     /gene="infB"
                     /locus_tag="VIX62_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY5858969.1"
                     /translation="MRVHELAKELGVTSKELLGTLEQMGVSGKSASSSVPEDLVPRLR
                     ASGGKATEAPKRREVLEPPPAPRKPKPKKAAAPKAEPKPADVAPAPVAEAPAPVAAPA
                     SPAPAAAAPAPAAPAAVPAPAADAAAAPPNGRQPRGPRKPVVPEPPRPVLEVVRGATP
                     QTIAEKVGKSPADIVKLLFLAGEMATATTSLTDEAITMVASELGLSAEIVGIEDEMQG
                     IEEIEEEVDPSLLAPRPPVVTVMGHVDHGKTKLLDAIRETDVVAGEFGGITQHIGAYQ
                     AHVGDRDITFIDTPGHEAFTAMRARGAQVTDIAVLVVAADDGVMPQTIEALDHAKAAG
                     VPIVVAVNKVDKEEADPNRVRTQMVEQGVVPSEWGGTNEFVDVSAREKLNLDNLLETI
                     LLVADLEELKGVPSGPAKGTVIEAHLDKGRGPVATVLVQRGTLDVGDALVCGTTYAKI
                     RAMQDEYGQTVEHAGPSKPVVILGWEAVPSSGDEFREVSDEREARHIASEREAKVRAA
                     ELVISRPPTLSDLLRQAERAEIPELNLVVKADVQGSLGALTDAFLKLPQDEVRVNIVR
                     SAAGGITENDITLAMASRAIVVGFNVRPDKAAVDLADKEGVDIRTYRVIYDAIDDIKA
                     ALSGMLAPEKQEHELGSAEIREIFRVPRIGVIAGCYVTNGTIPRDARARLVRDGVVVY
                     EGKIGSLRRFKDDVREVAAGYECGISIDGFQDVKEGDLIEAFEIREVARSL"
     gene            8014..8307
                     /locus_tag="VIX62_01630"
     CDS             8014..8307
                     /locus_tag="VIX62_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEY5858970.1"
                     /translation="MLVALERFDLRIRESHSLKQKRHVVKTLTNGIRSTFNVSVAEVD
                     HQDLWQRTAIAVAVVGAQEYHLRKVLHEIEQYVERWAEVELLEAEVSVHYEEE"
     gene            8331..8687
                     /gene="rbfA"
                     /locus_tag="VIX62_01635"
     CDS             8331..8687
                     /gene="rbfA"
                     /locus_tag="VIX62_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY5858971.1"
                     /translation="MTQGARTERVGEEFREILAEQIQRLKDPRVGFITVTGVRVSPDL
                     RHAWVSYTALGDDKARAGSRAAIRSATPHLRSALGKQVRLKFLPELEFEEDQTFERSQ
                     QIDALIEELHRKERTE"
     gene            8684..9667
                     /locus_tag="VIX62_01640"
     CDS             8684..9667
                     /locus_tag="VIX62_01640"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEY5858972.1"
                     /translation="MSNDNLERAGVVLAGATEVALACHVNPDADALGSMLGLAAFLRS
                     RGVRTVCSYPNEPLDPPRWAALLPGSEDLVPVRDYPKQPAVMVTCDCAAFDRLGALGH
                     PAEHAGELIWIDHHRSNDGRGTIPLVDPDASSTCEMVFRLIETMGGAMPDETALCLYA
                     GLVTDTGRFQYEATTPETLRVAAKLREHPFEHAKLVQALYEDNLESYLKLVGTALGRL
                     EHVEDADLVWTYLTQSDLREAGIHPSETDDLIDVVRTARDVDVAAIVKQQRDGRFKVS
                     VRSRGDHDLSQVAATFGGGGHRLAAGYTSAHGPAETIDRLSRALRGEPVAP"
     gene            9664..>9790
                     /gene="truB"
                     /locus_tag="VIX62_01645"
     CDS             9664..>9790
                     /gene="truB"
                     /locus_tag="VIX62_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884094.1"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY5858973.1"
                     /translation="MTSPGPGTLDGFLLIDKPQGITSHDAVATVRRALGTKKVGHA"
BASE COUNT         1644 a         3289 c         3515 g         1342 t
ORIGIN      
        1 ccaggagccc gatagggggt acgggcgccg acgccggatc gggcaccgga tggccgtcga
       61 gcaactcggc cgcttcgaac gcgaaatagc ccatcagccc gcccgtgagc ggtggaagcc
      121 ctggcaggcg cggggcccgc agggatctcg cgatccggat cagcccttcg cgcaccgggc
      181 caccatcggg cacgtcgatg gggatcgtgc gcgctcgttc ggtgacctcg atgccgtcag
      241 ggccggcgac gatcgtggct gccgggtcgc cggccacgaa cgagtaccgg ccccaccgtt
      301 cggagcgctc cacggactcg aacaggaccc caggaccgtc gccggtcagt gccgggaaca
      361 gtccgaccgg ggtcgagacg tcggacagga gctcggccca cacgggaacc agcggccact
      421 cggccgcgag cgcgtcgaac gtctcgcgat cgggacggac ctgcagttcc acgatgaccc
      481 tcctcgggaa acgaacgacc ccggaccttc cggcccgggg ttcgcggtgg tgccgtggtg
      541 aagcggtggc tacggacgcg cggacgccgg gctctcagag ccccggtgcc agacccagcg
      601 gtcgccacgc gtcgtcatgg ccggagattg tgacggctcg tgggccgggg cgtcaatcac
      661 gcatcatctg ccgcatcgag gaacagcggc cgttcggggt tccctcaagg gcaacgcggt
      721 gctagggtgc cgcgaaacgc tcgaaacccc cagaacacca acaggaggaa cgttcatggt
      781 gccggccccc cgctcggcac gacgaccgcg ttcgacgcgt cgccgtgctc tgaccatcgc
      841 tccgatcgcc gcggccctgg tcgccgtgct cgcccagccc gccctggcgg ccggttcgat
      901 ctccagctgg ggtgggaact cctacggaca gctcggggac ggcacgttca cgggccacgg
      961 ttcggccacc gcgatcgcga gcctcaccag catggtgtcg ctccacggtg gacgcgacca
     1021 cgtggccgcg ctgaagtccg acggcacggt gtggacctgg gggcggaacc agtacggcca
     1081 gatcggcgac ggcaccggca cgagccggcc caaccccacg ttggtcacgg gcctcaccgg
     1141 ggtgatcgcg gtcgagaccg gccactacaa caccatggcc ctcaagtccg acggcaccgt
     1201 ctggatgtgg ggctggaaca agtacggcac caacggcgac ggcacgacga cgaagcgcac
     1261 ccggccggtc ctggtgaaga acctgacgaa cgtggtggcg atcgcgggcg gtcgcgacca
     1321 gatgctggcg gtcaagtccg acggcacggt gtgggcctgg ggctacaacg ccttcggcaa
     1381 cctgggcgat ggcacgttca ccacacgtac cacccccgtg caggtcagtg gcctcgccgg
     1441 ggcgatcgac gtggcgggcg gacgtgacca ctccctggtg ctgaagtcag acggcacggt
     1501 ctggtgcttc ggctacaacg tccacggcga atgcggcgtc ggcaacacca gcaacaagaa
     1561 cctgccggtg caggtcagtg gcctcaccgg cgtcacggcg atcaccgcgg gggccaacca
     1621 ctccgtggcc ctgaagtccg acggcacggt gtggacctgg ggccagggct cgaacggccg
     1681 gctcggcaac aactccaccg cgaaccgcct gaccccggtg caggtctcga acctcaccgg
     1741 cgtggtcgcg atcggcacgg gccggatgca caccctcgcc gccaagtccg acggcacggt
     1801 gtgggcctgg ggcttcaacg ccacgggaca gctcggcaac ggcaacacca cgaaccagaa
     1861 ggtcccggtg ttggtgcagg ggttgaccgg cgccaccatg ttcggtggcg gtcgggacca
     1921 cagcgtcgcg ctcgtgccgt aagccgcatc ggtcgcccgg ctgcgccctg acggggccgc
     1981 tatcctgtgc ccctcatgcg actgtcgcgt tcgttcacgc ccacgctccg cgaggatccg
     2041 gccgaagccg agatcgccag ccatcgcctg ctcctgcggg gggcgttcgt gcggcaggtg
     2101 gctgcgggca tctacacgtt cctgccgatg ggccttcgga cgatgcggcg gatcgaggcg
     2161 atcatccgcg aggagatgga ggattcgggc gcgatcgaga tccgcatgcc gatcgcgatc
     2221 ccggccgagc cctggaaggc gaccggccgg tgggacctct acggcgacca gctcttccga
     2281 ttgcaggacc ggcacgggcg cgacatgctc ctcggtccca cccaggaaga ggtcgtggcc
     2341 ccgctggtgg cgggcgaggt ctcgagctac cgggacctcc cgctgaacct ctaccaggtg
     2401 gagtggaagt accgcgacga gttccggccg cggttcgggc tgctgcgagg acgcgagttc
     2461 ctgatgaagg acgcctattc cttcgaccgc gacgaacccg cgatggcggc ctcctacgag
     2521 gtgatgaagg ccgcctacca ccggatcttc gaccggtgcg gcctggacta cgcggtggtc
     2581 gaggctgagg ctggtcagat cggtggcggc accaaccacg agttcatggc acgggccgag
     2641 gtgggcgaag acctgttcgt cgagtgcgag cacggcgact acatcgccga cctgaaggcc
     2701 gctcgcccga tggcgcccga cgcctcggcc ccagccaccc aggagctcac cgtcgtcgac
     2761 acacccgaca cgccgacgat cgagacgctg gcggcgctcc tcgacatcga cgcctcgcag
     2821 acgctgaagt gtgtgctctt cgacgtgggc ggtcgcacgg tcgcggtgtt ggtgcccggg
     2881 gaccgcgagg tcaacggggc caagctggag cagctggtct tcccggcgac ggtccggatg
     2941 ttcgaggacg aggacttcgc ccggcacggg ttcgcgaagg gctacgtggg gccgcagggc
     3001 ttcgatgagg acgtcacgat ctacgccgac cacaccgtgc gcggcggcca tgattgggtc
     3061 accggttcga accaggtcga tcgtcacgtc accggcgcca acgcgggtcg cgacttccgc
     3121 gtggatgcct acgaggatct cgtcgtgctg cgtgaaggcg acgcgtgtcc cgtcgacggg
     3181 ggccagctcc acatcggccg ttcgatcgtg gtgggccaca tctaccagct gggcaagagg
     3241 tactccgaac cgctcgaggc caccttcacc gaggaggacg ggagcgagca ggtcttctgg
     3301 atggggtgct acggcatcgg gatctcccgg atcctggcgg cagcggccga gcagtgccac
     3361 gacgaggacg ggctgacgct ccccaaggtg ctcgccccgt tcgaggtggt ggtgatcctg
     3421 gcgaaccacg atgacgagca ggcgatcgcc gaggccgagc gcatctacca cgacctcacg
     3481 tctgcgggtg tcggcgtggc cttggacgac cgcgacgagc gcgcgggggt gaagttcgcc
     3541 gacgccgacc tgatcggcta cccagtgcag gtcacggtgg gcaagcgtgg gatcgccaac
     3601 ggcacggtgg acctgaagct ccggtcgacg ggggagcgca gcaacgcacc cctggccgag
     3661 gcggcccagg ccgcggtcgc gctcctcgca acggcaccgt agaccccgct ccgggggcgc
     3721 tgcgcggtgc ctcggctata ctgaccttcg tacagagcga cgaggtgtgt ccggaggtgg
     3781 gctcgcgccc accttctcgt acgtagggga gggtcgagcc gtggacgcgg aagcgctggt
     3841 cagacccgtg atcgagcagg ccggcttcga attcgtggag ctggccacca cccgcgagca
     3901 cggccgcaag gtcctgcgtg tggtggtcga tcgccccggc ggggtggacc tcgacgcgct
     3961 gtcgcagctg tccgcaacgg tctcgcggca cctcgatcag gagggctacg aggacggtcc
     4021 gtacggactc gaggtttcgt cgcccggcat cgaacggccg ctgaagcgcc ctgagcactt
     4081 cgcgcggtcg gtgggcgagc aggtgaaggt gaagacgacg gcgcccatgg ccgggtcgag
     4141 gacccacacg ggcacgctgg tctcggccga cgaggacgcg atcgtgatcg cgaccgccga
     4201 cggcgacgaa gggaccggcg atctgcgcgt gccgtatgcc gacatcgtgt cggcgagaac
     4261 ggtcgtcgac tgggacgccg aactgaagag gagcaacgca tgaacgccga gatgatcgcg
     4321 gcgctccgag agctggagcg cgagaagggc atcgccttcg aaacgatcct gacgggtctg
     4381 gccgaggcga tggcctccgc atacaagacg tggtggaaac aggagaaccc cgacgccgac
     4441 gacgacttcg tgggcttccg tgccgagctc gatcccgaga ccggcgacct gcgcatgtgg
     4501 aagcaggagc tggaggagat cgaggtgccg gctgccgagg agggcggcga ccccaccctg
     4561 gagatgaagg tcctcggcga agcagaggtg gaggtcaccg acgacttcaa aggccgtatc
     4621 ggcgcgcaga ccgccaagca ggtgatcttc cagaagctgc gtgacgccga gcgcgagatg
     4681 acctacgagg agttcgccgg tcgcgagggt gacgtcgtga cgggcatcgt gcagcagcag
     4741 gagcgtcgct acaccctgct ggacctgggc aaggtcgagg cgttgctgcc gcaggccgag
     4801 caggtgccgt ccgagcccta ccgtcacggg gaacgcctca aggcgttcat caccgaggtc
     4861 cgcaagggca cgaaggggcc gcagatcgtc gtgtcgcgtt cgcaccccgg cctgctgaag
     4921 gcgctgttcg cgatggaggt gcccgagatc gaagagggca tcgtcgagat ccggggcgtg
     4981 gcgcgtgaac ccggccaccg ttcgaagatc gcggtgatct cgaacgagcc aggggtggac
     5041 cccgtgggcg cctgcgtggg ccccaagggc tctcgcgtgc gcatggtcgt caacgagctc
     5101 cgcggcgaga agatcgacgt ggtcccgtgg tcggacaacc ccggcgagct ggtcgccaac
     5161 gcgctgcagc ccgccaaggt caaggaggtc cgcaccgacg aggccaccgg caccgcgatg
     5221 gtcatcgtgc ccgactacca gctgtcgctg gcgatcggga aggaaggcca gaacgcgcgg
     5281 ctcgccgctc ggctgaccgg ctggcgcatc gacatcaagt ccgagaccca ggttgcggac
     5341 gaggccaagg ccccggccgc cgcgcccgtc gctccgccgg ctgaggaggt cgccgagccg
     5401 gccaccgacg ccgaggccga gctcgaggtc gaggtcgccg aagaagtggt ggccgacgaa
     5461 gcggtcgagg agctcgcggc cgaggaagcc gcggtcgagg cgatcgacga ggtcgtggcc
     5521 gaggaggcgg aggcacccga aggggtgtga cgtggcgccc acgcgaacct gcgtcgggtg
     5581 tcgcgagcct tcctccaaga gcggcctgct ccggatcgtg cgctccgcgg aggcgggggt
     5641 gcagttggac ccggacggga cggcgccggg gcgaggggcc tacgtgcatc gccgcgccgc
     5701 ctgcctggag gccgcgggag ccgaaggggt gctggccagg gcgttgcgga cgggccttgg
     5761 cccggacgaa ctgggtaggc ttagagatca gatgaggaca ggagtgctgt gagggttcac
     5821 gaacttgcca aggaactcgg ggtcaccagc aaggagttgc tggggacgct cgagcagatg
     5881 ggggtgagcg gcaagtccgc ttcctccagc gtgcccgagg acctcgttcc tcgtctgcgc
     5941 gcctccggcg gcaaggccac ggaggcgccg aaacgccgcg aagtgctcga accgccgccc
     6001 gctccgcgca agcccaaacc gaagaaggcc gcggccccca aggccgagcc gaagcccgcg
     6061 gacgtcgcac cggcgccggt cgcggaggca ccggctcctg tcgccgcccc cgcatcaccc
     6121 gcgcccgccg ctgccgcgcc cgcaccggct gccccggctg ccgttcccgc gcctgccgcg
     6181 gacgctgcgg ccgccccgcc gaacggtcgc cagccccgtg gcccccgcaa gcccgtcgtg
     6241 ccggaaccgc ccaggccggt cctggaggtc gtgcgcggtg cgaccccgca gacgatcgcc
     6301 gagaaggtcg gcaagagccc ggccgacatc gtgaagctgc tgttcctggc cggagagatg
     6361 gccaccgcca ccacgtcgct caccgacgag gccatcacga tggtcgcgag cgagctcggc
     6421 ctcagcgccg agatcgtcgg catcgaagac gagatgcagg gcatcgagga gatcgaagaa
     6481 gaggtcgatc cctccctgct ggcaccgcgt ccgccggtgg tcaccgtcat gggccacgtg
     6541 gaccacggca agaccaagct gctggacgcg atccgcgaga ccgacgtcgt cgcgggcgag
     6601 ttcggtggca tcacgcagca catcggcgcc tatcaggcgc acgtcggcga ccgcgacatc
     6661 acgttcatcg acacgcccgg ccacgaggcg ttcacggcga tgcgtgcccg cggcgcgcag
     6721 gtcaccgaca tcgccgtgct ggtggtggcg gccgacgacg gcgtcatgcc gcagaccatc
     6781 gaggcgctcg accatgcgaa ggcggccggc gtgccgatcg tggtcgccgt caacaaggtg
     6841 gacaaggaag aggccgaccc gaaccgcgtg cgcacccaga tggtcgaaca gggtgttgtg
     6901 ccctccgagt ggggcggtac gaacgagttc gtcgatgtct ccgctcgcga gaaactcaac
     6961 ctggacaacc tcctcgagac gatcctgctg gtggccgacc tcgaggagct caagggcgtg
     7021 ccgtccggtc ccgcgaaggg cacggtgatc gaggcgcacc tggacaaggg ccgcggtccc
     7081 gtcgccacgg tgctggtgca gcgcggcacg ctggacgtgg gcgacgcgct ggtatgcggc
     7141 accacgtacg cgaagatccg cgcgatgcag gacgagtacg gccagacggt cgagcacgcc
     7201 gggccgtcca agcccgtcgt gatcctgggt tgggaagcgg tgccgtcttc gggcgacgag
     7261 ttccgcgagg tgtccgacga acgcgaggcc cgtcacatcg cgtccgaacg cgaggcgaag
     7321 gtgcgcgccg ccgaactggt gatcagccgc ccgcccacgc tgtccgacct gctccgccag
     7381 gccgaacgcg cggagatccc cgagctcaac ctcgtggtga aggccgacgt gcagggctcg
     7441 ctcggtgcgc tcaccgacgc gttcctgaag ctgccgcagg acgaggtccg ggtgaacatc
     7501 gtgcgcagcg ccgccggcgg catcaccgag aacgacatca cgctggccat ggcgtcgcga
     7561 gccatcgtcg tgggcttcaa cgtgcgcccc gacaaggccg ccgtcgacct tgcggacaag
     7621 gaaggcgtcg acatccgcac gtaccgcgtg atctacgacg cgatcgatga catcaaggcg
     7681 gcgctctcgg gcatgctcgc gccagagaag caggagcacg agctcggctc ggccgagatc
     7741 cgtgagatct tccgggttcc ccggatcggc gtcatcgccg gctgctacgt cacgaacggc
     7801 acgatcccgc gggatgcccg cgcgcgcctg gtgcgtgacg gcgtggtcgt ctacgagggc
     7861 aagatcggct cgctccggcg gttcaaggac gacgtccgag aggtcgctgc gggctacgag
     7921 tgcgggatca gcatcgacgg cttccaggac gtgaaggaag gcgacctgat cgaggccttc
     7981 gagatccgcg aggtcgcccg ctccctgtaa gccatgctgg tcgccctcga acggttcgac
     8041 ctgcgcatcc gcgagtcgca ttccctgaag cagaagcgcc acgtcgtcaa gacgctgacc
     8101 aacggcatcc ggtccacgtt caacgtgagc gtcgccgagg tggaccacca ggacctgtgg
     8161 cagcgcaccg cgatcgccgt cgccgtggtc ggtgcccagg aatatcacct ccgcaaggtg
     8221 ctccacgaga tcgagcagta cgtggagcgc tgggcggagg tcgagctcct cgaggccgag
     8281 gtctcggtcc actacgagga ggagtgaccg gtcggcggta cgctgggtcc atgacccaag
     8341 gcgcacgcac cgaacgcgtc ggcgaggagt tccgcgagat cctggccgaa cagatccaga
     8401 ggttgaagga cccccgggtg gggttcatca ccgtgaccgg ggtccgcgtc tcgccggacc
     8461 tgcgtcacgc gtgggtctcc tacacggcgc tcggcgatga caaggccagg gccggcagcc
     8521 gcgcggcgat ccggtcggcg accccgcatc tgcgcagcgc cctgggcaag caggtgcggc
     8581 tgaagttcct gccggaactg gagttcgagg aggaccagac cttcgagcgg tcccagcaga
     8641 tcgacgcgct catcgaggag ctccaccgga aggagcgaac cgaatgagca acgacaacct
     8701 cgaacgcgcc ggcgtggtgc tggcaggagc caccgaggtg gcgctcgcgt gtcatgtgaa
     8761 tcccgatgcc gacgcgttgg gctcgatgct gggcctggcg gcgttcctgc ggtctcgggg
     8821 cgtccgtacc gtgtgctcct atccgaacga gccgctcgat cctccgcgct gggccgcgtt
     8881 gctgcccgga tcggaggacc tggtccccgt ccgcgactac cccaagcagc cggccgtgat
     8941 ggtgacgtgc gactgcgcgg ccttcgaccg cctgggcgcg ttgggccacc ccgcggagca
     9001 cgcgggcgag ctgatctgga tcgaccacca ccgctcgaac gacggccgag gcacgatccc
     9061 gctcgtcgat cccgacgcat cctccacctg cgagatggtc ttccggctga tcgagacgat
     9121 gggcggggcg atgcccgacg agacggcgct gtgtctctac gccgggctgg tgaccgacac
     9181 cggccgcttc cagtacgagg caaccacccc ggagaccctc agggtggccg cgaagctgcg
     9241 tgagcatccg ttcgagcacg cgaagctggt ccaggcgctc tacgaggaca acctggagtc
     9301 ttacctgaag ctggtgggca cggcgctcgg ccgcctggag cacgtcgagg acgccgacct
     9361 ggtctggacc tacctcacgc aatcggatct gcgagaagcc gggatccacc cgtcggagac
     9421 cgacgacctg atcgacgtgg tgcgcaccgc gcgcgacgtg gacgtcgccg cgatcgtgaa
     9481 gcagcagcgc gacggacgtt tcaaggtgag cgtgcgctcg cgcggcgatc acgacctctc
     9541 ccaggtggcg gcgaccttcg gtgggggtgg gcaccgactg gcggccggct acacctcggc
     9601 gcacggtccg gccgagacga tcgaccggct gtcgagggcg ctgcgcggcg aacccgtcgc
     9661 cccgtgacct ccccgggacc cgggaccctt gacgggttcc tgctgatcga caagccgcag
     9721 gggatcacgt cccacgacgc ggtggcgacg gtgcgacgcg cgctgggaac gaagaaggtt
     9781 gggcacgcgg
//
LOCUS       DATDBZ010000065         8445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1249201, whole genome shotgun sequence.
ACCESSION   DATDBZ010000065 DATDBZ010000000
VERSION     DATDBZ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249201"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VIX62_01650"
     CDS             complement(<1..788)
                     /locus_tag="VIX62_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEY5858974.1"
                     /translation="MRPRELTIEGFRSYRGATTFDWRDRRLVGIVGPIGAGKSSILDA
                     VAFALYGKTPGIGSATKSLIHQQCTEAHVSLTFEVDGQIWQAVRAPKRKGQSGHQLRH
                     LAADEPDAEVLETVTMEGAVNERITHLLGMDFDTFCRSVLLAQNRFSDFLKATKTDRD
                     KVLKGVFGFEQIDAAKLAAERRLDRETMALEAFAREREGIDQARERLEEARGLAAQTR
                     SRLKAFEDCAPRVQELVAQEAAARSDAEVAASRIDELARVADTLP"
     gene            complement(775..1941)
                     /locus_tag="VIX62_01655"
     CDS             complement(775..1941)
                     /locus_tag="VIX62_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEY5858975.1"
                     /translation="MKILHTSDWHIGKRLGRHDRMGEFAEVLAEVETIADERAVDLVL
                     VSGDIWDRPMPPTDALHLGLQTLLRLAQGRPVVAVAGNHDSAGLFEALAPLLRPLGVF
                     LIGDVKRPDQGALLGPDVLGVPAVVAGFPFLREGRVVDFMAEAGEWYRAYAERVAAIT
                     RTYNEALVAQAGGDAVPILMAHFMVGGVAIDRGAPRGERELHMGDAYAATAQAIPAGP
                     QYVAMGHIHAPQAVPGAPVPAHYAGSLLALDFGEAGEQKRVVIVDAEPGQLATVESIP
                     LTKGRPLIRVTDDWDAIEARAPELAEAFLDLTVRTSGTDMTLADRAGEAFPYLVKVRA
                     LRPEGERPQHVAKGGRSKDQLYAEYYEREHGEPAPEELLTLFRDVLEEAADASA"
     gene            complement(1958..3697)
                     /locus_tag="VIX62_01660"
     CDS             complement(1958..3697)
                     /locus_tag="VIX62_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5858976.1"
                     /translation="MTDERQVGRVLGSEHSNTGAFRVVLDEHDFLQLDDLVVVRTQVP
                     KAGEVRTYGVVTEAEAVYEGASYESDTHRIAELGIMPAAKVRTARIAVTRVDPEVWVS
                     PDPGESVERAEGDERDKALYVDEMSRPLPVGIGRDELPVHVDLDFFDGRKGGHMSISG
                     ISGVATKTSFALFFLRMLTAHPQILGEGAANLRVLVFNVKGEDLLWLDKPNRLFDEQA
                     AAGWASLGVEATPFPSVCFWAPPKRKSGDVLLPDTGGRHEGVEVFTWTPREFIDDDLM
                     QFLLTDANDQRNQIPFVRERVQAQLKRFAVDVAGRPGAVVLRDPRDSQNGSWYGQQVN
                     VAAGERIITDLQSLVDGIGEFLEPDDGTEPDFAWSGRVMGGTISAFMRRMHAGASRIG
                     PLVRAGESHRIDRARASVSVVAIQSLHDQAQRFVVGALLNETFAEKEATGQRLPLSVI
                     VLDELNKYAPREGHSPLKEMLTDIAQRGRSLGVLLVGAQQTASRVAQEVLENAAIRVS
                     GRLDAAEAERAEYGWMLPSTRARARLLKPGTMVVSQPAIPVPLVVTFPFPPWATRKEE
                     VAADADGDPFAGL"
     gene            complement(3694..4647)
                     /locus_tag="VIX62_01665"
     CDS             complement(3694..4647)
                     /locus_tag="VIX62_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858977.1"
                     /translation="MARIFVEGWDPEYGTPLDQDEALAPAEGSVDPTVETDDWEPLEG
                     MDDGIEHVAFVDGVRRVDARLTLDDPVAGPTPGLVGTFAVGATRWDRPARRSEITAVR
                     IERWAVLAGGREEQMPPVALEPAVNTTTCASEDPGRLMMELHTKMRRAEGETATALAA
                     ECFVIADGPLNDLTAHPTVGYVKSHRVTYLEPDHNAVVAQLAPGQRTPLFCIADYKRY
                     SWYVRLAMLDSGHSWTGIVRCETSGQLPKEEAVTIADRTAALLPLVASQPHVDPRAPQ
                     NLVPIGALEKELRHRMGDRGIVYRALREAVMERRGIEQEAS"
     gene            complement(4744..5514)
                     /locus_tag="VIX62_01670"
     CDS             complement(4744..5514)
                     /locus_tag="VIX62_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858978.1"
                     /translation="MRRLPLVLAVAAFVAVLMTSCDTDQPIGVPVTPLPSGSILPSVT
                     TQPSVPASQSATASPSPDLPAGVPVSYDLDVRKEDVPSKALIPPGTKVSNRWMTETPG
                     GEVIVVAYLTPSGDPFLQARGLVEWRRTEGSVPPWSPVFGMEHRKSEGILGVEGLVGD
                     ATGDGSVDLLLLESTGGSGVCGNWRVIDLAAASERWVRSLCDGRVDLHVDPVGIALLE
                     AVYGPEDPHCCPSGFRTTVLVYQEGRGFVKDSVQSTPA"
     gene            5573..6091
                     /locus_tag="VIX62_01675"
     CDS             5573..6091
                     /locus_tag="VIX62_01675"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY5858979.1"
                     /translation="MSEPTLPPKRVTDSTVTLTQMMEVTDANIAGKVHGGVIMRLVDT
                     AAALAAIRHAGGLAVTVAIDEMTFLDPVSIGEVIVLKASVNAVGHSSMECGVRVDALD
                     PVTGRTRHANSAYLVFVAVDDAGQPRPVPPLLVETDEEKRRQREAMLRRETRLAHKEA
                     VKAAREIEEAQP"
     gene            6088..6390
                     /locus_tag="VIX62_01680"
     CDS             6088..6390
                     /locus_tag="VIX62_01680"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019748908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY5858980.1"
                     /translation="MILEHAVLQVKPGTQAEFTDAFDRARAIIEAAEGFNSLLLLPCV
                     EDETRYLLLVEWEHLEDHTEGFRGSPAYEEWKTLLHHFYDPFPEVDHYPLPDLDDD"
     gene            6471..>8445
                     /gene="acnA"
                     /locus_tag="VIX62_01685"
     CDS             6471..>8445
                     /gene="acnA"
                     /locus_tag="VIX62_01685"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY5858981.1"
                     /translation="MTSELAGARGRLETADGPIDLYRLDAIGVDLSRLPKTVLILLEN
                     LLRRAGSRDVSDEDVRALAAWPGPAGDVAFMPGRVLMQDFTGVPAVVDLAAMRSATQR
                     AGADPASINPSIPVDLVIDHSVSVDLFRSPEAYARNIEFEYRRNGERYSLLRWAQQAF
                     EGLRVVPPGAGICHQVNLEHLGQVVSTRDGVAFPDTLVGTDSHTTMINGLGVLGWGVG
                     GIEAEAAMLGQPMFLPQPVVIGVRMTGALLAGTTATDLVLTLTEMLRAHGVVGKFVEF
                     FGDGLSSLSIADRATLSNMCPEYGATASYFPVDAETMRYLTFTGRGDRVDLAERYTKE
                     QGMFRRDGDPEPTFSEILDLDLASVVPSMAGPKRPQDRVALPDVWDSFLAAFRDHLEP
                     DPKGVEIGRFVAEGGSLRNEELPGDEDVADHPAGESIVGHGSVVIAAITSCTNTSNPS
                     VMLAAGLLARKAIEAGLDTKPWVKTSLAPGSRVVTDYLDRAGLTPYLDKLGFALVGYG
                     CTTCIGNSGPLIDDVAKAVDEHDLNVAAVLSGNRNFEGRVHPQVRASYLASPPLCVAY
                     ALAGRIDVDLTTEPLGEGSDGPVFLADIWPSADEVRDAMATAITESQYTTQYERIWSG
                     DEHWQGLPTPSGPVYDWDPVSTYVQEPPFFDDLG"
BASE COUNT         1298 a         2890 c         2962 g         1295 t
ORIGIN      
        1 ggcagggtgt cggcgacgcg agccagttcg tcgatccggc tggcggccac ttccgcatcg
       61 gaccgcgctg ccgcctcctg ggccacgagc tcctggacgc ggggggcaca gtcttcgaag
      121 gccttcaacc gggagcgggt ctgcgcggcg agcccgcgag cctcctccag ccgttcgcgg
      181 gcctgatcga tgccctcgcg ctcgcgggcg aacgcttcca gggccatcgt ctcccggtcg
      241 agccggcgct cggcggcgag cttggccgca tcgatctgtt cgaagccgaa cacacccttg
      301 aggaccttgt cgcgatcggt cttcgtggcc ttcaggaagt cgctgaaccg gttctgcgcc
      361 agcagcaccg accggcagaa cgtgtcgaag tccatgccga gcagatgcgt gatgcgttcg
      421 ttgacggcgc cttccatcgt gacggtctcg agcacctcgg cgtcgggctc gtccgcggcc
      481 aggtgcctga gctgatgacc ggactggccc ttgcgcttgg gcgcccgtac cgcctgccag
      541 atctgtccgt cgacctcgaa cgtgagcgaa acgtgcgcct cggtgcactg ctggtggatc
      601 agggacttgg tggccgaacc gatgcccggc gtcttgccgt agagcgcgaa cgcgaccgca
      661 tccaggatcg aggacttccc ggcaccgatg ggacccacga tgcccaccag gcgacgatca
      721 cgccaatcga acgtggtggc acctcggtag gagcggaagc cctcgatcgt gagctcacgc
      781 ggacgcatcg gccgcttcct cgagcacgtc gcggaacagg gtgagcagtt cctcgggcgc
      841 tggttcgccg tgctcgcgtt cgtagtactc ggcgtagagc tggtccttgc tccgaccgcc
      901 tttggcgacg tgctgggggc gctcgccctc cggccggagc gcccgcacct tgaccagata
      961 cgggaacgcc tcgcccgcgc ggtcggcgag ggtcatgtcg gtgccggagg tccgaacggt
     1021 gaggtcgagg aacgcctcgg ccaactccgg agcgcgggcc tcgatcgcgt cccagtcgtc
     1081 ggtgacccgg atcagcgggc gtcccttcgt gagcgggatg ctctcgacgg tggcgagctg
     1141 gccgggttcg gcatcgacga tcacgacccg cttctgctcg ccggcttcac cgaagtccag
     1201 ggcaaggagc gaacccgcgt agtgggccgg cacgggcgcg ccgggcaccg cctgcggcgc
     1261 gtggatgtga cccatggcga cgtattgcgg gccggcgggg atcgcctggg cggtggccgc
     1321 gtacgcgtcg cccatgtgga gctcgcgctc gccgcgcggc gccccgcgat cgatcgcgac
     1381 cccgcccacc atgaagtgcg ccatcaggat cggcacggcg tcgccgcccg cctgcgccac
     1441 gagcgcctcg ttgtacgtgc gggtgatcgc cgccacacgc tcggcgtatg cgcggtacca
     1501 ctcgccggct tcggccatga agtcgaccac gcgaccttct cgcaggaacg ggaaccctgc
     1561 caccacggcg ggcaccccga ggacatccgg acccagcagc gcgccctggt cgggtcgttt
     1621 cacgtcgccg atcaggaaca ccccgagggg ccggagcagg ggagcgaggg cctcgaacaa
     1681 cccggcggag tcgtggttgc ccgccaccgc cacgacggga cgcccctggg ccaggcggag
     1741 cagcgtctgc aggccgagat gcagggcatc ggtgggtggc atcgggcggt cccagatgtc
     1801 gccggacacc aggaccaggt ccacggcgcg ttcgtcggcg atcgtctcga cttcggcgag
     1861 cacctcggcg aactcgccca tgcgatcgtg gcggccgagc cgcttgccga tgtgccagtc
     1921 cgacgtgtgc aggatcttca tggggggagt ggagacgtta caggcccgcg aacgggtcgc
     1981 cgtccgcgtc ggccgcgacc tcctccttcc tggtcgccca gggtgggaag gggaaagtga
     2041 cgacgagcgg caccgggatc gcgggctgcg acaccaccat cgtgcccggc ttcagcaacc
     2101 gggcgcgagc ccgggtggac gggagcatcc agccgtattc ggcgcgttcg gcttcggcgg
     2161 cgtccagacg accgctcacc cggatcgcgg cgttctcgag cacctcctgc gccacgcggc
     2221 tcgcggtctg ctgcgcgccg accagcagga cgccgagcga acggccccgc tgggcgatgt
     2281 cggtgagcat ctccttcaac ggcgagtggc cctcgcgcgg ggcgtacttg ttgagctcgt
     2341 cgagcacgat caccgacagc ggcaggcgct gaccggtggc ttccttctcg gcgaacgtct
     2401 cgttgagcag cgcgcccacg acgaagcgct gcgcctgatc gtgcagcgac tggatcgcca
     2461 cgacgctgac gctggctcgc gcgcggtcga tgcgatggga ctcgccggcg cgcaccaatg
     2521 ggccgatgcg cgaggcgccc gcgtgcatgc gtcgcatgaa cgcgctgatg gtgccgccca
     2581 tgacgcgtcc gctccaggcg aagtcgggtt cggtgccgtc atcgggttcc aggaactcgc
     2641 cgatgccgtc caccagcgac tggaggtcgg tgatgatgcg ctcgccggcc gcgacgttga
     2701 cctgctggcc gtaccacgag ccgttctgcg agtcgcgagg gtcgcgcaac acgaccgcgc
     2761 cgggccggcc cgccacatcg accgcgaacc gtttgagctg tgcctgtacc cgctcacgca
     2821 cgaaggggat ctggttgcgc tggtcgttcg cgtcggtgag caggaactgc atgaggtcgt
     2881 cgtcgatgaa ctcgcggggc gtccaggtga agacctcgac gccctcatgg cggccgccgg
     2941 tgtcgggcag cagcacgtcg ccggatttcc gtttgggcgg cgcccagaag cacacgctgg
     3001 ggaacggcgt ggcctcgacg ccgagcgagg cccagcccgc ggcggcttgc tcgtcgaaca
     3061 gccggttggg cttgtcgagc cacagcagat cctcaccttt gacgttgaag accagcacgc
     3121 gcaggttggc ggcgccctcg cccaggatct gcggatgcgc cgtgagcatg cgcaggaaga
     3181 acaacgcgaa cgaggttttg gtggcgacgc cgctgatgcc gctgatcgac atgtggccgc
     3241 ccttgcgtcc gtcgaagaag tcgagatcga cgtgcaccgg aagctcgtca cggccgatcc
     3301 ccacgggcag cggccggctc atctcgtcga cgtagagcgc cttgtcacgt tcgtcgccct
     3361 cggcacgctc gaccgattcg ccggggtcgg gcgagaccca cacctcgggg tcgacgcgcg
     3421 tgacggcgat gcgcgcggtg cgcaccttcg cggccggcat gatgccgagt tcggcgatgc
     3481 ggtgggtgtc ggattcgtag ctggcgccct cgtagacggc ctcggcctcg gtgacaacgc
     3541 catacgtccg gacctcgccg gccttcggca cctgggtgcg caccacgacc aggtcgtcca
     3601 gctggaggaa gtcgtgctcg tcgagcacca cgcggaaggc gccggtgttg gaatgctccg
     3661 aaccgagaac ccggccgacc tggcgttcgt cggtcacgag gcctcctgct cgatgcctcg
     3721 ccgttccatg acggcctcgc ggagcgcccg gtacacgatg ccgcgatcgc ccatgcggtg
     3781 gcggagctcc ttctcgagcg caccgatcgg caccaggttc tgcggcgcgc gcggatcgac
     3841 gtgcggctgg gaggcgacca acggcagcaa cgcggcggtg cgatcggcga tcgtgacggc
     3901 ctcctccttg gggagctgcc ccgatgtctc acaccgaacg atcccggtcc aggaatgccc
     3961 gctgtcgagc atcgcgaggc gcacgtacca ggagtaccgc ttgtagtcgg cgatgcagaa
     4021 cagcggcgtg cgttgaccgg gcgcgagctg agcgacgacc gcgttgtgat cgggttccag
     4081 gtaggtcacg cggtggctct tcacatagcc gaccgtcggg tgggcggtga ggtcgttgag
     4141 cggtccgtcg gcgatcacga agcactcggc ggcgagcgcg gtggcggtct cgccctcggc
     4201 gcggcgcatc ttcgtgtgga gctccatcat caggcggccg ggatcctcgg atgcgcacgt
     4261 ggtggtgttg accgcgggct cgagcgcgac gggcggcatc tgctcctcgc ggccgccggc
     4321 cagcaccgcc cagcgttcga tgcgcacggc ggtgatctcg gagcgacggg cgggccggtc
     4381 ccagcgggtg gcgcctaccg cgaacgtccc cacgagcccc ggcgtgggtc ccgcgaccgg
     4441 atcgtcgagc gtgagccgag cgtcgacccg ccggacgccg tcgacgaacg cgacgtgctc
     4501 gatgccgtcg tccatgcctt ccaggggttc ccagtcgtcg gtctcgaccg tgggatcgac
     4561 cgaaccttcg gccggagcga gggcctcgtc ctggtcgagc ggggtgccgt actcggggtc
     4621 ccaaccctcg acgaagatgc gagccatggc gcgtatcgta cccgggcacc ccgacagccc
     4681 tgtggagccg gatccagagc ttcccgtgca actagtcttc gccaaaactc gtgctcagct
     4741 tgctcaggcg ggggtggact gcacgctgtc cttcacgaac ccccggccct cttgatagac
     4801 caggacggtg gttcggaatc cgctcggaca gcaatggggg tcttcgggac cgtagacggc
     4861 ctcgaggagc gcgatgccga cgggatccac atgcaggtcg acccgcccat cgcagagtga
     4921 tctcacccag cgctccgacg ccgctgccag gtcgatgact cgccagttgc cgcagacacc
     4981 gctgccgccg gtcgactcca gcagcaagag gtcgacggat ccgtctcctg tcgcgtcgcc
     5041 gacgagccct tcgacgccga ggatcccctc gctcttgcgg tgttccatcc cgaacacggg
     5101 cgaccagggc gggaccgacc cctcggtccg acgccactcg acgagaccgc gggcctggag
     5161 gaacggatcg ccggacggcg tgaggtaggc cacgacgatg acctcgcccc caggcgtctc
     5221 cgtcatccat cggttgctga ccttcgtccc cggtgggatg agcgccttcg aagggacgtc
     5281 ctccttacgg acgtccaggt catatgacac gggaaccccg gccgggaggt cgggcgaagg
     5341 gctggccgtc gccgattgcg atgccggcac ggacggctgg gtcgtgacgg aaggcaggat
     5401 ggaacccgac ggaagcgggg tcaccggaac cccgatgggc tggtcggtat cgcaggaggt
     5461 catgagcacg gccacgaacg cggccacggc gagcacgagg ggaaggcgtc gcatcgctcg
     5521 atgctaccgg cgcccaggcc gccgccgggg gagccgcggg taggatgcgc gcgtgagtga
     5581 gcccaccctg ccaccgaaac gcgtcaccga ctccaccgtg acgttgacgc agatgatgga
     5641 ggtcaccgac gccaacatcg ccggcaaagt gcacggtggc gtgatcatgc gcctggtcga
     5701 caccgccgcc gcactcgccg cgatccggca cgcgggcggc ctcgccgtca ccgtggcgat
     5761 cgacgagatg accttcctgg atcccgtgtc gatcggcgag gtcatcgtgc tcaaggcctc
     5821 cgtgaacgct gtcggacaca gctcgatgga atgcggggtc cgggtggacg ccctcgaccc
     5881 ggtgaccggg cggacgcggc atgcgaacag cgcgtacctg gtgttcgtgg cggtcgacga
     5941 cgccgggcaa ccccggcccg ttcctccgct cctggtcgag accgacgagg agaagcgccg
     6001 ccagcgcgag gcgatgctgc gtcgggagac ccgcctggcc cacaaggaag cggtcaaagc
     6061 cgctcgcgag atcgaggagg ctcagccgtg atcctggaac acgccgtgct gcaagtgaaa
     6121 ccgggcacgc aggccgagtt caccgacgcg ttcgaccgag cccgagcgat catcgaggcc
     6181 gcggaggggt tcaactccct gctgctgctt ccctgcgtcg aggacgagac ccgatacctg
     6241 cttctggtcg agtgggagca cctggaggat cacaccgaag ggttccgagg atcaccggcc
     6301 tacgaggagt ggaagacgct gctccaccac ttctacgacc ccttcccgga ggtcgatcac
     6361 taccccctgc ccgacctcga cgacgactga gcccggctga ttctcggtgc cttcgcgctg
     6421 cgtgggagga tggaaggaca cctctcacct ggaaggactg acatcacacg atgaccagcg
     6481 aactcgcggg ggcgcggggg cggttggaga ccgccgacgg cccgatcgac ctgtaccggc
     6541 tcgatgcgat cggcgtggac ctgtcgcgac tgccgaagac cgtgctgatc ctgctcgaga
     6601 acctgctgcg gcgcgcaggt tcgcgcgacg tctccgacga ggacgtgcgc gcgctcgccg
     6661 cgtggcccgg gcccgcgggc gacgtcgcgt tcatgccggg ccgcgtgctg atgcaggact
     6721 tcacgggcgt gcccgccgtg gtggacctgg ccgcgatgcg cagcgctacc cagcgagcgg
     6781 gcgccgatcc cgcttcgatc aacccatcga tccccgtgga cctggtgatc gaccactccg
     6841 tgtccgtgga cctgttccgt tcgcccgagg cctacgcgcg caacatcgag ttcgagtacc
     6901 ggcggaacgg cgagcgctac tcgctcctcc ggtgggccca gcaggcattc gaaggattgc
     6961 gcgtggtgcc gcccggcgcg ggcatctgtc accaggtgaa cctggaacat ctggggcagg
     7021 tcgtctcgac gcgcgacggc gtggcgttcc ctgacacgct cgtgggcacc gactcgcaca
     7081 ccacgatgat caacgggctc ggcgtgctgg ggtggggcgt gggcggcatc gaggccgaag
     7141 ccgcgatgct gggccagccg atgttcctgc cacagcccgt ggtgatcggc gtgcgcatga
     7201 ccggtgcgtt gctggccggg accaccgcca ctgacctcgt gctgacgctc accgagatgc
     7261 tccgcgcgca cggcgtggtg ggcaagttcg tggagttctt cggtgacgga ctctcgtcgc
     7321 tgtcgatcgc ggaccgagcc acgctgtcga acatgtgccc ggagtacggc gccaccgcgt
     7381 cctacttccc ggtggacgcc gagacgatgc gctacctcac cttcaccggc cggggcgatc
     7441 gcgtcgacct ggccgagcgc tataccaagg agcaggggat gttccggcgc gacggcgatc
     7501 ccgagccgac gttcagcgag atcctcgacc tcgacctggc gtcggtggtg ccctcgatgg
     7561 ctgggccgaa acggccgcag gaccgcgtgg cgctcccgga cgtgtgggac tcgttcctcg
     7621 cggcgttccg cgatcacctc gagccggacc cgaagggcgt cgagatcggg cggttcgtcg
     7681 ccgagggtgg gtcgctgcgc aacgaagagc tccccgggga cgaggacgtc gcggatcatc
     7741 ccgcgggcga gagcatcgtc gggcacggca gcgtcgtgat cgccgcgatc acgtcctgca
     7801 cgaacacctc gaaccccagc gtgatgctgg ccgccgggct cctggcgcgc aaagcgatcg
     7861 aggcgggcct ggacaccaag ccgtgggtga agacgtcatt ggctcccggc tcacgcgtcg
     7921 tcaccgacta cctcgaccga gccgggctca ccccgtatct cgacaagctc gggttcgcgt
     7981 tggtgggcta tggctgtacc acgtgcatcg gcaactccgg tcccttgatc gacgacgtcg
     8041 cgaaggccgt ggacgagcac gacctcaacg tggcggcggt gctcagcggc aaccggaact
     8101 tcgaagggcg ggtgcatccg caggttcgtg cctcctacct ggcatcgccg cccctatgcg
     8161 tggcgtacgc cctcgcgggg cggatcgacg tcgacctcac gaccgaaccg ctcggggaag
     8221 gaagcgacgg gccggtgttc ctggccgaca tctggcccag cgccgacgag gtgcgcgatg
     8281 cgatggcgac ggcgatcacc gagtcgcagt acacgaccca gtacgaacgc atctggagcg
     8341 gcgacgaaca ctggcaggga ctgccgacgc cgtcgggccc cgtctacgac tgggaccccg
     8401 tctccaccta cgtgcaggaa ccgccgttct tcgacgacct cggca
//
LOCUS       DATDBZ010000066         3231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2680889, whole genome shotgun sequence.
ACCESSION   DATDBZ010000066 DATDBZ010000000
VERSION     DATDBZ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2680889"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="VIX62_01690"
     CDS             <1..173
                     /locus_tag="VIX62_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018725369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5858982.1"
                     /translation="EPSDRARDDEIERVVALGATPLLDLRKDDGSGWLVLADPEGNEF
                     CILRSRAEREQH"
     gene            complement(288..863)
                     /locus_tag="VIX62_01695"
     CDS             complement(288..863)
                     /locus_tag="VIX62_01695"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002928932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY5858983.1"
                     /translation="MTTRCAWAPLDDPAYLRYHDEEWGVPVHDDVHLFEMLVLEGAQA
                     GLSWSTILHKRDGYRRAFAGFDPAKVARFSPAKVERLLQDPSIVRNRLKVASAVQNAK
                     AVLDVQRDDGSLDAYLWSFVGGTPKRNRWRGTGAIPAETEESRSMSKDLKRRGFRFVG
                     PTVCYAFMQATGMVNDHVANCFRFTELRDAT"
     gene            complement(860..1990)
                     /locus_tag="VIX62_01700"
     CDS             complement(860..1990)
                     /locus_tag="VIX62_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5858984.1"
                     /translation="MKIAPFEMERYQSRYWHQVDHDLSESGVLPMTIRELLGPTADAE
                     GFLHTALGYPLSEGSLETRASIAAWYPGASPEAVSITNGGSEANFLTLFSLLGSGDRL
                     AFMLPNYLQGRGLGKAFGKATDTFRLKPQDGRWALDLEGLDDAVGKKTKVVMVCNPNN
                     PTGSVLTEDEMDAIVRVAERRGAWIVADEIYRGAELDTDVTSPTFWGRYDKVIITSGL
                     SKAFALPGLRVGWVVGPTEVIEQVQAHHDYTTLSPGMVSDRLAALAMLPAVRESIFAR
                     TRTIIRTNYPYMETWLSSHADVMTWTRPEAGAIVYAHVDLPVKSRDLVERFRTEQSVL
                     LVPGEMFGLKKGIRFGFGFDIDATMKGLGRVDETLAQMAAGA"
     gene            2076..3047
                     /locus_tag="VIX62_01705"
     CDS             2076..3047
                     /locus_tag="VIX62_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5858985.1"
                     /translation="MGRTVRILREADVREALDMASCIDACERAFEAYSNGGAELPSVI
                     HLDVPESKGEIHVKAGHLHGQPYYAVKVASGFYDVDPAAIDGLVMVFDASNGAPAAFL
                     LDGGFITDLRTGAAGGVAARHLAPEHVEVVAVIGTGAQARHQVDALGHVRQGLAQVRV
                     WGRDPDRAAACADDLRSHVVESCAVNAAASVEEAVEGADVVITCTAAREPLVRADLLA
                     PGAHVTALGSDGAGKQELDPEILRRADVLVVDSVDQCLALGELQHAPEQAERAVELGR
                     ICAGEHPGRTGEGQLTVCDLTGLGVQDVAAANVVMARAGDRGDTLDL"
     gene            3044..>3231
                     /locus_tag="VIX62_01710"
     CDS             3044..>3231
                     /locus_tag="VIX62_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858986.1"
                     /translation="MTRTRQDRTSDEWAVLLEPLARRALASYDLDVADVSLITNDWNC
                     VFRVDLADGSSRVLRVSLP"
BASE COUNT          501 a         1121 c         1094 g          515 t
ORIGIN      
        1 tagaaccctc tgaccgtgcc cgcgatgacg agatcgagcg tgttgtggcc ctcggtgcga
       61 cgccgctcct cgatctccgg aaggacgatg ggagcggctg gctggtcctc gccgatccgg
      121 agggcaacga gttctgcatc ctccgtagcc gagccgagcg cgagcagcac tgaagcgtcc
      181 acctccaggt acctacccga acgctctcgc tccatggcgt cctgttcctc gatgagtttc
      241 acggagttcc ggcgcgatgc gatcgaagag ctgcgccagc tgctggatca ggtcgcgtcg
      301 cgcagctcgg tgaagcggaa gcagttcgcg acatggtcgt tgaccatgcc cgtcgcttgc
      361 atgaacgcgt agcagaccgt cggccccacg aatcggaacc ctcggcgctt caggtcctta
      421 gacatcgatc gggactcctc ggtctcggcg gggatggccc cggtcccacg ccaccggttc
      481 cgtttcggcg tgccgccgac gaacgaccag aggtaggcgt cgagcgagcc atcgtcgcgc
      541 tgcacgtcca gcaccgcctt cgcgttctgc accgcggatg ccaccttcaa gcggttacgg
      601 acgatcgacg gatcctgcag cagccgctcc accttcgcgg gtgagaaccg cgccaccttc
      661 gccggatcga acccggcgaa cgcccgccga tagccatcgc gcttgtgcag gatcgtggac
      721 cagctcaggc cggcctgcgc accttccagc acgagcatct cgaacaggtg tacatcgtcg
      781 tggacgggca cgccccattc ctcgtcgtgg tagcgcaggt acgcgggatc gtccagcgga
      841 gcccacgcgc agcgtgtggt cacgcgccgg cagccatctg cgccagcgtc tcgtcgacgc
      901 gtcccagccc tttcatcgtg gcgtcgatgt cgaagccgaa cccgaaccgg atccccttct
      961 tcagaccgaa catctcgccg ggcaccagga gcacgctctg ctccgtgcgg aaccgctcga
     1021 ccaggtcgcg gctcttgacc gggaggtcga cgtgcgcgta gacgatcgcg ccggcctccg
     1081 gccgcgtcca ggtcatgacg tcggcatggc tcgagagcca ggtctccatg tacgggtagt
     1141 tggtgcggat gatcgtccgg gtgcgggcga agatcgactc gcgcaccgcg ggcaacatcg
     1201 cgagggccgc gaggcggtcc gagaccatcc cgggtgagag cgtcgtgtag tcgtgatggg
     1261 cctgcacctg ctcgatcacc tcggtgggtc ccacgaccca ccccacgcgg agccccggaa
     1321 gggcgaacgc cttcgacagg ccgctcgtga tgatcacctt gtcgtagcga ccccagaacg
     1381 tcggcgaggt gacatcggtg tcgagctcgg cgccccggta gatctcgtcg gccacgatcc
     1441 acgcgccgcg gcgttcggcc acgcgcacga tcgcatccat ctcatcctcg gtcagcaccg
     1501 aaccggtcgg gttgttcggg ttgcagacca tgaccacctt ggtcttcttg cccacggcgt
     1561 cgtcgagccc ctccaggtcc agcgcccacc ggccgtcttg gggcttgagc cggaacgtgt
     1621 ccgtcgcctt gccgaacgcc ttgccgagcc cgcgaccctg gagatagttc ggcagcatga
     1681 aggcgagacg gtcgccggag ccgagcagcg agaacagggt caggaagttg gcttcggaac
     1741 cgccattggt gatcgacacc gcttcgggcg aggcgccggg gtaccaggcc gcgatgctgg
     1801 cgcgcgtctc gagtgagcct tccgacaggg gatatccgag cgcggtgtgc aggaagcctt
     1861 cggcgtcggc cgtcggtccg aggagctcgc ggatcgtcat cgggagcacg cccgactccg
     1921 agaggtcgtg atcgacctgg tgccagtagc gggactggta gcgctccatc tcgaacggcg
     1981 cgatcttcac gggacctcct gggggacggg gtcccgcgaa cctagcaccg cccgagcggg
     2041 actgctccgg cccgtgccga taggcttcga tcacgatggg tcgaaccgtc cggatcctgc
     2101 gcgaagccga cgtccgcgag gcgctcgaca tggcctcgtg catcgacgcg tgcgagcgcg
     2161 cgttcgaggc ctactccaat ggtggcgcgg agctgccgtc agtgatccac ctcgatgtcc
     2221 cggagtccaa gggcgagatc cacgtgaagg ccggacacct gcacggccag ccgtactacg
     2281 cggtgaaggt ggcgagcggc ttctacgacg tcgaccccgc cgccatcgac ggcctcgtga
     2341 tggtgttcga cgcctcgaac ggtgcgccgg cggcgttcct gttggacggg gggttcatca
     2401 ccgacctgcg gaccggcgcg gcgggcgggg tcgccgcccg ccacctggcg cccgaacacg
     2461 tcgaggtggt ggcggtgatc gggaccggcg cgcaagcgag gcaccaggtc gacgcgctcg
     2521 ggcacgttcg ccaagggctc gcacaggtcc gcgtgtgggg ccgcgacccc gatcgcgccg
     2581 ccgcctgtgc ggacgacctg cgttcgcacg tcgtcgaatc gtgcgcggtc aacgccgccg
     2641 cgagcgtcga ggaagccgtc gagggcgccg acgtcgtcat cacctgcacc gccgcacgcg
     2701 agccgttggt ccgagcggac ctgctcgctc cgggcgcgca cgtcaccgcg ctcggttccg
     2761 acggtgccgg caaacaggaa ctggacccgg agatccttcg acgcgccgac gtcctggtgg
     2821 tcgactccgt cgaccagtgc cttgcgctcg gggaactgca acacgcccct gagcaagccg
     2881 aacgtgcggt cgagctcggc aggatctgtg ccggcgagca cccgggtcgc acgggcgagg
     2941 gccagctcac ggtgtgcgac ctcaccggcc tgggcgtgca ggacgtcgcg gccgccaacg
     3001 tcgtgatggc gcgtgccggc gatcgcggcg acacgctcga cctgtgacgc gaacgcggca
     3061 ggaccgcacc agcgacgagt gggccgtgct gctcgagccc ctcgcccgtc gtgcccttgc
     3121 gtcctacgac ctcgacgtgg cggacgtctc gctcatcacc aacgattgga actgcgtgtt
     3181 ccgtgtggac ctggccgacg gatcatctcg cgtgctccgc gtcagtctcc c
//
LOCUS       DATDBZ010000067         3252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2681551, whole genome shotgun sequence.
ACCESSION   DATDBZ010000067 DATDBZ010000000
VERSION     DATDBZ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2681551"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /gene="nuoB"
                     /locus_tag="VIX62_01715"
     CDS             complement(<1..257)
                     /gene="nuoB"
                     /locus_tag="VIX62_01715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEY5858987.1"
                     /translation="MEDEIERGVLLTSLDRAVGWARKQSMWPATFGLACCAIEMMSTG
                     AGKYDLSRWGMELFRASPRQADLMIVAGRVSQKMAPVLRRI"
     gene            complement(400..723)
                     /gene="ndhC"
                     /locus_tag="VIX62_01720"
                     /pseudo
     CDS             complement(400..723)
                     /gene="ndhC"
                     /locus_tag="VIX62_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018217175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
     gene            complement(770..2020)
                     /locus_tag="VIX62_01725"
     CDS             complement(770..2020)
                     /locus_tag="VIX62_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEY5858988.1"
                     /translation="MSAQRIDTDVLVVGGGPGGAAAGYHLARQGIDVTVVDKASFPRE
                     KVCGDGLTPRSVAALLRMGIDTDNPGFERVKGLRVYSRRATIELPWPELSSWPGYGLV
                     MPRAEFDSLLIERAQKAGARLMERTDAVEPIFDGGWVVGAKVRPAEDREAEPSEIRAR
                     FVIAADGAASRFGKPAGIQRDDSRPLGIAARRYYRTDYHPGPWFESWLDLWEGDLLLP
                     GYGWLFPVAGGQINLGAGLLNTFKDFKQISAQRLFNAFATMLPAEWQISEETAEGRVL
                     SGPLPMSLNRVPQVVPGMLLIGDAAGAVNPFNGEGIAYAMETAEIAAELIDEALVKDR
                     PGIAMMYPTVLRERYGDYFRIGRGFAKIIGRPKIMGAATRVLLPNPKVMGFAMRLMAN
                     LTDGKDGDAQDRLFAALERIARAS"
     gene            complement(2206..2631)
                     /locus_tag="VIX62_01730"
     CDS             complement(2206..2631)
                     /locus_tag="VIX62_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5858989.1"
                     /translation="MSDDLDVLRRMFRDQPACRVGTVRPDGGPYVAVRWFVWLDDEIW
                     VSTRSGDSTWEHVLRDPRVSIVIDRGRDWAELAGVRIEGVAEMFPAEHPDLRGPMSAW
                     HEKYRSMLGGDSFERLTRDVVSLGFLRVAPSRVDAWDHR"
     gene            complement(2628..>3252)
                     /locus_tag="VIX62_01735"
     CDS             complement(2628..>3252)
                     /locus_tag="VIX62_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5858990.1"
                     /translation="ELLHTMALVHDDLLDHTTERRGEPTTAPTMAAAGLVLAPSIDPR
                     TFGTSAAILVGDLAAVLADRLLLESGFEPAALERALDVYHRMRIETAVGQFLDVAGHG
                     AEPARAREVARLKGGAYTVEGPLLVGAAFADGGADVRAALGRYGRALGEAFQLRDDLH
                     DEDAASGVTADEVNGLVERARIALGQAPVDPDAARVLEAMAGSVEMA"
BASE COUNT          578 a         1174 c         1004 g          496 t
ORIGIN      
        1 tagatgcggc gcaggaccgg cgccatcttc tgcgacacgc ggcccgccac gatcatgagg
       61 tcggcctgac ggggcgatgc acggaacagc tccatccccc accgcgacag gtcgtacttt
      121 cctgcaccgg tcgacatcat ctcgatggca caacacgcca gcccgaacgt ggccggccac
      181 atcgattgct tccgtgccca ccccacggcg cggtcgagcg acgtcagcag gaccccgcgt
      241 tcgatctcgt cttcgatctc ctccggggtc ttgcccaggt ggacaccttc gagccgctca
      301 acgggcaccg gccacctcca cgtcgtcctg tgcgctcagt gctgcctcgg gatcctcggc
      361 cgccatcgcc accgactcca ccggcgtcac ggtgtcggcc caatcgaggc ccccgctgcg
      421 ccagacgtag acgtaggcca cgaacaccag cccgatgaag atccccatct ccaccaggcc
      481 gaacatcccc agacgccgga acgacacggc ccacgggaac aggaagatcg tctcgatgtc
      541 gaagatgatg aacagcatcg cgaccacgta gaacttgacg ctgaacttct cccccttcgg
      601 caaccggacg gggtcgttgc cgcactcgta ggcggagagc ttgacggggt tcgggcgatt
      661 cgggcggaac caccgcgaca cgctcatgga cacgacgccg aacacgaccg cgagcacggc
      721 catcgccacg atcggcaggt agtcgatcag cacgggcgaa tcctacccat catgaggcgc
      781 gggcgatccg ttcgagggcg gcgaacaggc ggtcctgcgc gtcgccatcc ttgccgtcgg
      841 tgaggttggc catgaggcgc atcgcgaagc ccatgacctt gggattgggt agcaggaccc
      901 gcgtcgccgc gcccatgatc ttgggtcggc cgatgatctt ggcgaacccg cgaccgatgc
      961 ggaaatagtc gccgtagcgc tcgcgcagca cggtcggata catcatcgcg atgccgggtc
     1021 gatccttcac gagtgcctcg tcgatcagct cggccgcgat ctccgccgtc tccatcgcgt
     1081 aggcgatacc ttcgccgttg aaggggttca ccgcaccggc ggcatcaccg atcaacagca
     1141 tgcccggtac gacctgcggc acgcggttca aactcatcgg cagaggcccc gacagcaccc
     1201 ggccttccgc cgtctcctcc gagatctgcc actcggcggg cagcatcgtc gcgaacgcgt
     1261 tgaagagccg ctgcgccgag atctgcttga agtctttgaa ggtgttcagc aggccggcgc
     1321 ccaggttgat ctgcccaccg gccaccggga acagccatcc gtagccgggc aacaagaggt
     1381 cgccttccca caggtcgagc cacgattcga accaggggcc tgggtgatag tcggtgcggt
     1441 agtagcgacg cgccgcgatc cccagcggcc gggaatcgtc gcgctggatc cccgcgggtt
     1501 tgccgaatcg gctggcagcg ccgtcggcag cgatcacgaa ccgcgcccgg atctcgctgg
     1561 gttcggcctc cctgtcctcg gcgggtcgga ccttcgctcc caccacccaa cctccgtcga
     1621 agatcggctc gacggcatcg gtacgttcca tcaatcgggc gccggccttc tgggcgcgtt
     1681 cgatgagcag cgagtcgaac tcggcgcgcg gcatcacgag gccgtagccg ggccatgaag
     1741 acagttcggg ccacgggagc tcgatcgtgg ctcggcggga gtagacgcgg aggcccttga
     1801 cccgttcgaa cccagggttg tcggtgtcga tccccatgcg caggagggcg gcgacgctgc
     1861 ggggcgtcag cccatcccca cacaccttct cccgagggaa gctggccttg tcgaccaccg
     1921 tgacatcgat gccttggcgg gcaaggtggt agccagcggc ggcaccgcca ggtccaccgc
     1981 ccactaccaa cacgtcggtg tcgatccgct gtgcgctcac gcgctcccct tcagctgggc
     2041 gactcgccgg tccacggccg ccgatcggaa acctttgtga aaagtttcac aaggtgaggc
     2101 taccgcatca ccctccaacg tgccgaggct acgacgggtt ccccagggag cgcaaaccag
     2161 aagtcgcgaa ccacgaaccg cggaggaacg ctgcgatctg ccaggtcagc ggtgatccca
     2221 ggcgtccacg cgagaagggg caacgcgcag gaaaccgagg ctgacgacgt cacgcgtcaa
     2281 tcgttcgaac gaatcgcccc ccaacatcga ccggtacttc tcgtgccagg ccgacatcgg
     2341 cccccgcagg tccggatgtt cggccgggaa catctccgcg acaccttcga tccgcacgcc
     2401 cgccaactcg gcccaatcac ggccgcgatc gatcacgatc gagacccgtg gatctcgcag
     2461 gacgtgttcc caggtgctat cacccgagcg cgtcgacacc cagatctcgt cgtcgagcca
     2521 cacgaaccag cggaccgcta catacgggcc tccgtcggga cggacggtgc ccacccggca
     2581 tgccggctga tcgcggaaca tccggcgcaa cacatcgaga tcgtcgctca tgccatctcc
     2641 accgaaccgg ccatggcctc gagcaccctc gcggcgtcgg ggtccacagg cgcctggccc
     2701 aacgcgatcc tcgcgcgctc gacgagaccg ttcacctcgt ccgcggtcac gccggaagcg
     2761 gcgtcctcgt cgtgcaggtc atctcgaagt tggaacgctt cgcccaaggc tcggccatat
     2821 cgcccgaggg ccgcccgaac atcggcccca ccatcggcga aggcggcgcc gaccaacagc
     2881 gggccttcca ccgtgtaggc gccgcccttc aaccgcgcca cctcgcgagc ccgtgccggc
     2941 tcagcgccgt ggcccgccac gtccaggaac tggcccaccg ccgtctcgat ccgcatgcgg
     3001 tggtagacgt ccagcgcgcg ttccaaggct gcgggttcga acccgctctc cagcagcagt
     3061 cgatccgcga gcaccgctgc aaggtcgccg accaggatgg ccgccgacgt cccgaaggtc
     3121 cggggatcga tcgacggggc caggacgagg cccgctgctg ccatcgttgg ggccgtcgtg
     3181 ggttcacccc gtcgttctgt ggtgtggtcg agcagatcgt cgtgcaccaa cgccatcgtg
     3241 tgcaacaact cc
//
LOCUS       DATDBZ010000068         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_841077, whole genome shotgun sequence.
ACCESSION   DATDBZ010000068 DATDBZ010000000
VERSION     DATDBZ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841077"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..411)
                     /locus_tag="VIX62_01740"
     CDS             complement(19..411)
                     /locus_tag="VIX62_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5858991.1"
                     /translation="MVSLKDFRCGASAAVSDMPRARQFYEGVLGLVPGTDTGDNVGYP
                     CGGDTQINVYLSPNAGTAAFTIAGFDVDDVEAVVDQLTAQGVTFERYDEPPIVTDAKG
                     IAHFEGDAKVAYFKDPDGNILSLAQAPV"
     gene            complement(443..1036)
                     /locus_tag="VIX62_01745"
     CDS             complement(443..1036)
                     /locus_tag="VIX62_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018844031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY5858992.1"
                     /translation="MTTEYKLDMLMMYAVHDAFRRDLERVAEMTERSEGWDAFEHFLR
                     LHHEVEDDALWPDLRETLVGRADDLVLLDEMEAEHAVLGPLLDAIDNALDRRESAPGA
                     RADLAAGLQEHLTHEEEAALPLIDRTFDEDQWMQFGAAAAAKVGPDMPTYLPWLLHGV
                     DGERANDVLGQLPQPVQQDYRDEWQPAYAANDWWAVP"
     gene            1131..1775
                     /locus_tag="VIX62_01750"
     CDS             1131..1775
                     /locus_tag="VIX62_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5858993.1"
                     /translation="MSQLLRVQCFNVSRDGFGTGEGQSLEHPFGDADAADLFAWAGAT
                     ASWVNRTDPGGSRGLDDYFTRDFANNIGAEIMGRNKFGPVRGPWEDHDWKGWWGDTPP
                     FHTPVFVLTHHDRPSFTLSDTTFHFLDASPAEALREAKQAADGQDVRLGGGVTTIREF
                     LDADLVDTMHIAVAPVELERGERLWTSHEELLDRFHLETVPSPSGVTHLLFWRR"
BASE COUNT          322 a          686 c          681 g          341 t
ORIGIN      
        1 cagacggatc ccttcctgtc aaaccggggc ttgggcgagg gagaggatgt ttccgtccgg
       61 atccttgaag taggcgacct tcgcgtcgcc ttcgaagtgc gcgatgcctt tggcgtcggt
      121 gacgatcggc ggttcgtcgt agcgctcgaa ggtcaccccc tgcgcggtga gctggtcgac
      181 aacggcttcg acgtcgtcga cgtcgaagcc ggcgatcgtg aacgccgcgg tgcccgcgtt
      241 cggcgacagg tacacgttga tctgcgtgtc acccccgcac gggtacccga cgttgtcgcc
      301 cgtgtcggta ccgggcacga gcccgagcac cccttcgtag aactggcgtg cccgcggcat
      361 gtcggatacg gcagcggacg cgccacagcg gaagtccttc aggctgacca tcacgtcctc
      421 ctcgctacgt gggctccgcc cctcatggaa cggcccacca gtcgttggcg gcgtaggcag
      481 gctgccactc gtcccgatag tcctgctgga ccggttgggg tagctggccc agcacgtcat
      541 tggctcgctc gccgtcgacg ccatgcaaca gccacgggag gtaggtgggc atgtcggggc
      601 cgaccttggc cgcggccgcg gcgccgaact gcatccactg gtcctcgtcg aaggtgcggt
      661 cgatgagggg caaggccgcc tcctcctcgt gagtgaggtg ctcctggagc ccggcggcca
      721 gatcagcccg cgctccgggc gccgactcgc ggcggtcgag ggcgttgtcg atggcgtcca
      781 gcagcggtcc gagcacggca tgctcggcct ccatctcgtc gaggagcacc aggtcgtcgg
      841 cgcgaccgac cagcgtctca cgcaggtccg gccacagcgc gtcgtcctcg acctcgtgat
      901 gcaggcgcag gaagtgctcg aacgcgtccc agccctcgga gcgttccgtc atctccgcga
      961 cccgctccaa gtcgcgccgg aatgcgtcgt gcacggcgta catcatcagc atgtcgagct
     1021 tgtactcggt ggtcatgctg cgcactgctc ccacagaggg cgacgggttc gagaccgaac
     1081 ctacccacgg caggctggac tcaaaacgaa gagaaagagg agatggagga atgtcacaac
     1141 tgctgcgcgt gcagtgcttc aacgtctcgc gggacgggtt cggcacaggt gaaggccaga
     1201 gtctggaaca cccattcggg gacgccgatg ccgctgatct cttcgcctgg gccggcgcca
     1261 cggccagctg ggtgaatcgc acggatcccg gtggttcccg cgggctcgat gactacttca
     1321 cccgcgactt cgccaacaac atcggcgccg agatcatggg ccgcaacaag ttcggaccgg
     1381 tgcgcggccc gtgggaggac cacgactgga agggctggtg gggcgacacc ccgccgttcc
     1441 acaccccggt gttcgtgctg acccaccacg accgcccctc gttcacgctg tcggacacca
     1501 ccttccactt cctcgacgca tccccggccg aggccctgcg cgaggcgaag caggccgcgg
     1561 acggtcagga cgtacgcctc ggtggcggcg tcacgaccat ccgggagttc ctcgacgcgg
     1621 acctcgtcga cacgatgcac atcgccgtcg cgccggtcga gctcgaacga ggcgaacggc
     1681 tgtggacctc ccacgaggag ctgctcgatc gcttccacct cgaaaccgtg cccagcccca
     1741 gcggggtgac gcacctgctg ttctggcggc ggtgaccgcg acggttgcgc tcagccctgg
     1801 tagatccaga tccagttggc gctcggatcc ttcacggccg agttgcgagg cattcccggg
     1861 ggtcatggag attcccttct cgaggaagta gtcgacgtct tcgatctggc cggcgacgag
     1921 gctctctctg cgctcgagac gcgcgcggtg ccgccgaagg atctcgttgg ttgacgtgcc
     1981 ggtggcgtcg gcgagcgcgg cgcggaacac acggcgtccg ctcaggtgat
//
LOCUS       DATDBZ010000069         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2274133, whole genome shotgun sequence.
ACCESSION   DATDBZ010000069 DATDBZ010000000
VERSION     DATDBZ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274133"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VIX62_01755"
     CDS             complement(<1..779)
                     /locus_tag="VIX62_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5858994.1"
                     /translation="MIFGLAAALGWGLADFGGAIAGRRIGSIVTVIVGQVLSVTFMTV
                     LLLATGESTAAIRPILGWIALNGVFTAVAYMTHYRALELGPVAVVSPIGAAYAVVGVA
                     LAIVVLDERPAALQVVGAVITVFGVMLVSTDLKKLRAGTHGVPPGLPWAIVAAFSFGV
                     AAFLLGFASKEAGWIAGLWASRAAQVACYVPVAFLYRRQLAALRPGPGLWVALAAGAA
                     DILGVTTYAAGAERGYLSIVLAASAVFPMIAVLLSIRYLHER"
     gene            complement(776..>2113)
                     /locus_tag="VIX62_01760"
     CDS             complement(776..>2113)
                     /locus_tag="VIX62_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY5858995.1"
                     /translation="SIVAHTGALAGNTALRDAWLRGRGAVLVEDPVTMFEASVLFSHI
                     KKLRTTGVAAALQSGGACTLFAEAAGNAGLSLPGFAPATKRSLKKALPTFASQNNPLD
                     VTGQAAVETNMFEGALEALANDPSVGLVAFDAFPPRLPDETPWADPVLAKVVELQRRT
                     GVAFVSVAMSPLAYGPDAIAFTKRWKRLAFLQGHHASTGAIRALVDHHGVKARAFVAR
                     EPHEERTAARRVLRGLTGAVDEATGADLLGRYGVRRPKEATVTTPEEAAEVARRVGFP
                     VAIKALAPEIPHKARLGGVRLGIADAAEAQVAADEVLTAARRAGAARPRVLVQQMVTG
                     DEVLVGAVIDDRFGPFITMRPGGALAEVGEATFVPCPLTPAQARSFVADQAERCGLQP
                     DRHHVRAVAGAVESVARAAWDLRDRLISLEANPLLVDDRGAVAVDALAEVRTP"
BASE COUNT          371 a          788 c          674 g          280 t
ORIGIN      
        1 cgctcgtgca gataccggat cgacaacagc accgcgatca tcgggaacac ggcactcgcg
       61 gccaacacga tcgacaggta accccgttcg gcgcccgccg cgtacgtcgt cacgcccagg
      121 atgtcggcgg cgccggccgc cagcgccacc cacaggccgg ggccggggcg caacgccgcg
      181 agctgccgtc ggtagaggaa cgccaccggc acgtaacacg ccacctgggc cgcccgagaa
      241 gcccacagcc ccgcgatcca tccggcttcc ttcgacgcga accccaacag gaacgcggca
      301 acgccgaagc tgaacgccgc cacgatcgcc catgggagtc cgggtggcac accatgcgta
      361 ccggctcgca gcttcttgag atcggtcgag accaacatca caccgaacac cgtgatgacc
      421 gcccccacca cctggagcgc ggccggacgc tcgtccagca cgacgatggc gagcgccacg
      481 cccaccaccg cgtacgcagc cccgatcggc gagaccacgg ccacaggccc gagctccagc
      541 gctcggtagt gggtcatgta ggcgaccgcg gtgaagacac cgttcagggc gatccagccg
      601 aggatgggcc ggatcgcggc cgtggactcc ccggtcgcga gcaggagcac ggtcatgaac
      661 gtcaccgaca ggacctgacc gacgatgacc gtgacgatgc tgccgatcct gcggcccgcg
      721 atcgcccctc cgaagtcggc gagaccccac cccagcgcag cggccaggcc gaagatcacg
      781 gagtgcgcac ttcggcgagc gcgtcgacgg cgaccgcacc ccggtcatcg accagcagcg
      841 ggttcgcctc cagggagatc aatcggtcgc gcagatccca tgccgcacgc gctacgctct
      901 cgacggcacc ggcgacggcc cgaacgtggt gacgatccgg ctgcagaccg caccgctcag
      961 cctggtcggc gacgaacgat cgggcctgcg ccggtgtgag cggacacggc acgaacgtcg
     1021 cctcgcccac ttcggcgagg gctccccccg ggcgcatcgt gatgaaggga ccgaaccggt
     1081 cgtcgatgac tgcgcccacc agcacctcgt cgccggtgac catctgctgc accaacaccc
     1141 tgggccgggc ggcaccggca cgtcgggctg cggtcagcac ttcgtctgcc gcgacctgcg
     1201 cttcggctgc gtcggcgatc ccgagtcgaa cgccacccaa ccgggccttg tgcgggatct
     1261 ccggagcgag ggccttgatc gcgaccggga acccgacccg gcgagcgacc tctgccgcct
     1321 cctccggcgt ggtcaccgtc gcctccttcg ggcgacggac gccgtaccga ccaagcaggt
     1381 cagcacccgt cgcctcgtcg acagcgcccg tcaggccacg caacacgcgt cgcgcggccg
     1441 tgcgttcctc atggggttcc cgtgcgacga acgcccgggc cttcacccca tggtgatcga
     1501 cgagtgcgcg gatcgccccc gtcgacgcgt ggtgaccttg caggaacgcc aaacgcttcc
     1561 accgtttcgt gaatgcgatc gcgtcgggcc cataggcgag tgggctcatc gcaacgctca
     1621 cgaatgccac accggttcga cgctgcagtt cgacgacctt cgcgagcaca gggtcggccc
     1681 atggcgtttc gtcgggcaag cgagggggga acgcgtcgaa cgcgaccagc ccgaccgacg
     1741 gatcgttcgc gagcgcctcc agtgcaccct cgaacatgtt ggtctcgacg gcggcttggc
     1801 cggtgacgtc aagggggttg ttctgcgatg cgaatgtggg cagcgccttc ttcaacgacc
     1861 gcttcgtcgc aggggcgaac ccgggaagcg agaggcctgc gttccccgct gcttcagcga
     1921 ataacgtgca ggcgccgccc gattgcaatg ccgccgccac gccggtcgta cgcagcttct
     1981 tgatgtgtga gaaaagcacc gacgcctcga acatcgtgac cgggtcctcc accaataccg
     2041 ccccgcggcc gcgcaaccac gcatcccgca acgctgtgtt gcctgcgagc gcccccgtgt
     2101 gcgccacgat cga
//
LOCUS       DATDBZ010000070         4077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2274382, whole genome shotgun sequence.
ACCESSION   DATDBZ010000070 DATDBZ010000000
VERSION     DATDBZ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2274382"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..924
                     /locus_tag="VIX62_01765"
     CDS             109..924
                     /locus_tag="VIX62_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5858996.1"
                     /translation="MQTSNKTPIRHDHDLIVVPDARPPRRWGLIALVVAAILVALLVG
                     GAIGYSMRQADVRDLRAQLAGSASQQQVAVTDVGAALQRVDALERRIEDLRANMEVQR
                     RAVGVLRGSNHEKRRQVTQARKELEAAQAELRATTGPPLSDGTFAVKVVAVGASQSPS
                     RIVLDLGRWFTGAAAHDAAAADGLAAHNDRGNHPRYFRNREAGWRIMPLAPSATATFW
                     RWGSRLGPSTIAIADFQQLVHSGAPWAVRVSNDPFSATVQNGTVTGLRQLRYP"
     gene            1000..1935
                     /locus_tag="VIX62_01770"
     CDS             1000..1935
                     /locus_tag="VIX62_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5858997.1"
                     /translation="MTRYDDILAAIGNTPLVGMPRMSPKPGVRLWAKLEGNNPTGSTK
                     DRIALKMVETAEASGDLTPDKTILEPTSGNTGIALAMVAARKGYRLTVVIPDNASEER
                     IGLLRLFGAEIVFSDGEKGTNGSIEVARALAADDKYYMPFQYGNPANPMAHQEGTAQE
                     IIRDLPEVTHFIAGMGTGGTLTGNGRGLHAHNPDIKVIAAEPELGDLVYGLRSLDAGF
                     IPPIFDPEQVSRKFLVDSSNSLRTNRELTSKEGIFAGISSGAVVYVAQRIASEIDDGD
                     IVCLLPDGGWKYLSTEAWASDLETAEKGVAESLWW"
     gene            1948..2775
                     /gene="murI"
                     /locus_tag="VIX62_01775"
     CDS             1948..2775
                     /gene="murI"
                     /locus_tag="VIX62_01775"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY5858998.1"
                     /translation="MSDPRPIGMFDSGVGGLTVARAVLDLLPHEPLLYVGDTARSPYG
                     PRQPEEIRRYALEIADYLVHRDVKMLVVACNSVEVSAIGEIADRAHIPVVGVVEPGTR
                     AAARQTRNRIVGMIGTQATVRSGLYDRAMAATDARVTLHSNACPVFVEHVERGDTTSP
                     ELRGAARGYLSPLMAADIDTLILGCTHYPLLAGLLQRELGPGVVLVSSAEETAKDVYA
                     SLLDGDLQRVESSTPRHEFLATGDPAAFEQVAGVFLGPELTDVGAAQAQPVGGGSWN"
     gene            2766..3518
                     /locus_tag="VIX62_01780"
     CDS             2766..3518
                     /locus_tag="VIX62_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5858999.1"
                     /translation="MELTVLGTAGTWPPAGGATCGFLVSHEGTNIWLDAGTGTFARLQ
                     EHIGIDDVAAIVVSHGHTDHFIDVIPAFYARHYGHMGTPGLPFYSPEGFIDSVSLLVS
                     EDGKDVLREAYSFRTVGRGDAFEVGPFSISVFEMTHIGVNAVGYRIEAGGKVLAYTGD
                     TGPSEEVIDLARGADVFMAEATYQEASEKMFFHLSAVQAAEHAVRAGAKRLVLTHILP
                     TLDPEVSRAEAQGVFDGPVEIAYEGSKLEIGS"
     gene            3515..>4077
                     /gene="rph"
                     /locus_tag="VIX62_01785"
     CDS             3515..>4077
                     /gene="rph"
                     /locus_tag="VIX62_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY5859000.1"
                     /translation="MTRPDGRSPDQLRPVSWELGFQEWAAGSVLFSMGKTRVLVAASV
                     SDDAPRWLKGTGNGWVTGEYSMLPASTNERTNREVNRGRPGGRTQEIQRLIGRSLRSV
                     TDLSRLGERTITIDCDVLQADAGTRTASITGGYIALAMALRDLAAAGQIPDDLLTDSV
                     AAVSVGIVEGEARLDLCYDEDAGADVD"
BASE COUNT          654 a         1393 c         1419 g          611 t
ORIGIN      
        1 agcgccacga cgacggctcc tattggtggg tgttcgaata aatgtcgtat tgactccgaa
       61 tacaacgcag gactagcgtc cgcacgagga agggaggggc acttccccgt gcagacctcg
      121 aacaagacgc cgatccgtca cgaccacgac ctcatcgtcg tgcccgatgc gagaccaccc
      181 cggcgctggg gcctgatcgc cctcgtcgtg gccgcgatcc tcgtggcgct cctggtgggc
      241 ggcgccatcg gctactcgat gcggcaggcc gacgtgcgtg atctccgcgc ccagctcgcg
      301 ggctcggcga gccagcagca ggttgccgtc accgacgtgg gcgcggcgct gcagcgggtc
      361 gacgccctcg agcgaaggat cgaggacctg cgcgcgaaca tggaggtcca gcgacgcgcc
      421 gtcggggtgc tccgcggcag caatcacgag aagcgccgcc aggtcaccca ggctcggaag
      481 gaactcgagg cagcgcaagc cgagctccgg gccacgaccg gtccgcccct gtccgacggc
      541 acgttcgcgg tcaaggtcgt agcggtgggg gcctcgcagt cgccatcgcg catcgtcctc
      601 gatctgggtc ggtggttcac cggcgccgcg gcccacgacg ccgcggccgc ggacggcctc
      661 gccgcgcaca acgatcgcgg gaaccacccg cggtacttcc gcaaccgcga agccggctgg
      721 cgcatcatgc cgctcgcccc gtcggccacc gccaccttct ggcgttgggg ttcccggctg
      781 ggcccgtcca cgatcgcgat cgcggacttc cagcagctcg tccactcggg cgcaccctgg
      841 gcggtccggg tctcgaacga cccgttctcg gccaccgtgc agaacggcac cgtcaccggg
      901 ttgcggcagc tgcgctaccc gtaggtcatc ggccaacgac gcatcggatc gtcggcgctc
      961 ttggcgggac ggggcccgtc gcgctgcgat actccctggg tgaccagata cgacgacatc
     1021 ctcgccgcga tcgggaacac gccgctcgtg ggcatgccgc gcatgtcgcc gaaaccgggc
     1081 gtgcgcctgt gggccaagct cgaaggcaac aaccccaccg gttccaccaa ggaccgcatc
     1141 gcgctgaaga tggtcgagac ggccgaagcc tcgggcgacc tcaccccgga caagaccatc
     1201 ctggaaccca ccagcggtaa cacggggatc gcgctggcca tggtggccgc tcgcaagggc
     1261 taccggctca cggtggtgat ccccgacaac gccagcgagg aacgcatcgg gctgctgcgg
     1321 ctcttcggcg ccgagatcgt gttcagcgac ggcgagaagg gcacgaacgg ttcgatcgag
     1381 gtggcgcgcg cgctcgccgc cgacgacaag tactatatgc cgttccagta cgggaacccc
     1441 gccaacccga tggcccacca ggaaggcacg gcgcaggaga tcatccgcga cctccccgag
     1501 gtgacgcact tcatcgcggg catgggcacc ggaggaacgc tcaccggcaa cggccggggc
     1561 ctgcacgcgc acaaccccga catcaaggtg atcgccgccg agcccgagct cggcgacctg
     1621 gtctacggct tgcgctcgct cgacgccggg ttcatcccgc cgatcttcga tccggagcag
     1681 gtgagccgga agttcctggt ggactcgagc aactccctgc gcacgaaccg ggagctgacg
     1741 tcgaaggaag gcatcttcgc cggtatctcc agcggcgcgg tggtctacgt ggcccagcgg
     1801 atcgccagtg agatcgacga cggtgacatc gtgtgcctgt tgcccgacgg cgggtggaag
     1861 tacctgtcca ccgaggcgtg ggcgtccgat ctcgaaacgg ccgagaaggg cgtggccgaa
     1921 tccctgtggt ggtgaatgga tgagcgcgtg agcgatcctc gcccgatcgg catgttcgac
     1981 tccggggtcg gcggcctcac ggtcgcccgt gccgtcctcg atctgctgcc ccacgagccg
     2041 ctcctgtacg tcggcgacac cgcgcgttcg ccctacgggc cccggcaacc cgaggagatc
     2101 cgtcgctatg cgctcgagat cgccgactac ctggtgcatc gcgatgtcaa gatgctggtg
     2161 gtggcctgca actccgtgga ggtcagcgcc atcggcgaga tcgccgaccg ggcgcacatc
     2221 ccggtggtgg gcgtggtgga acccggaacc cgggccgcgg cgcggcagac ccgcaaccgg
     2281 atcgtcggca tgatcggcac gcaagcaacg gttcgcagtg gcctctacga tcgtgcgatg
     2341 gccgccacgg acgctcgcgt caccctgcac tccaacgcat gcccggtgtt cgtggaacac
     2401 gtggagcggg gggacaccac cagtccggag ctacgagggg ccgcgcgcgg gtatctttct
     2461 ccgctgatgg ctgccgacat cgacaccttg atccttggct gcacgcacta ccccctgttg
     2521 gccgggctgc tccagcgcga gctcggaccc ggcgtggtgc tggtctcctc ggccgaagag
     2581 accgccaagg acgtgtacgc ctcgttgctc gacggcgacc tgcagcgcgt cgaatcgtcg
     2641 acgccgcgcc acgagttcct cgccacgggc gaccccgcgg ccttcgaaca ggtcgcgggg
     2701 gtcttcctcg gacctgaatt gaccgatgtg ggcgccgcgc aggcccagcc cgtgggaggg
     2761 ggttcatgga actgacggtc ttgggcaccg cggggacctg gcctcccgcc ggcggtgcga
     2821 catgcggatt cctggtgtcc cacgagggca cgaacatctg gctcgatgcg gggacgggga
     2881 cgttcgcgcg gctgcaggag cacatcggca tcgacgacgt cgcggccatc gtggtcagcc
     2941 acggacacac cgaccacttc atcgatgtga tccccgcgtt ctatgcgcgc cactacgggc
     3001 acatggggac gccggggctg ccgttctact cgccggaagg gttcatcgac tccgtgagcc
     3061 tgttggtgtc cgaggacggg aaggacgtgc tgcgcgaggc gtattcgttc cggaccgtcg
     3121 gccggggaga cgcgttcgag gtggggccgt tctcgatctc ggtgttcgag atgacgcaca
     3181 tcggggtgaa cgcggtgggc taccggatcg aagcgggcgg caaggtgctg gcctacaccg
     3241 gcgacaccgg cccgtccgag gaggtcatcg acctcgcgcg cggcgccgac gtgttcatgg
     3301 cggaagccac ctaccaggag gcctccgaga agatgttctt ccatctgagc gccgtgcagg
     3361 cggccgaaca cgcggtgcga gccggcgcga agcgtctcgt gctcacgcac atcctgccca
     3421 cgctggaccc ggaggtctct cgcgccgagg cccagggcgt gttcgacggt cccgtcgaga
     3481 tcgcctacga gggctccaag ctggagatcg ggtcgtgacc aggcccgatg ggcgttcgcc
     3541 cgaccagctc cggcccgtga gctgggagct ggggttccag gagtgggcgg caggctcggt
     3601 cctgttctcc atgggcaaga cgcgggtgct cgtggcggcg tcggtctccg acgatgcgcc
     3661 ccggtggctc aagggcaccg ggaacggctg ggtgacgggg gagtactcga tgctgccggc
     3721 atccacgaat gagcgcacga accgcgaggt caaccgcgga cgtcccggtg gccgcacgca
     3781 ggagatccaa cggctgatcg gacggtcgct gcgcagcgtc accgatctgt cgcggctggg
     3841 ggagcgcacg atcacgatcg actgcgacgt gttgcaggcc gacgccggca cccgcaccgc
     3901 ctcgatcacc ggcggctaca tcgcgctggc gatggcgctg cgtgacctgg ccgctgccgg
     3961 tcagatcccc gacgatctgc tcaccgacag cgtcgccgcc gtgagcgtgg ggatcgtcga
     4021 gggcgaggcg agactcgacc tgtgctacga cgaggacgcg ggcgccgacg tcgactt
//
LOCUS       DATDBZ010000071         2168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1660199, whole genome shotgun sequence.
ACCESSION   DATDBZ010000071 DATDBZ010000000
VERSION     DATDBZ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660199"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VIX62_01790"
     CDS             complement(<1..305)
                     /locus_tag="VIX62_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY5859001.1"
                     /translation="MGSVDLERAARTAGLAAPVRWDEVTGSTNATAAIMAREGVPEWT
                     LVGAGHQTAGRGRRDRTWTDRPGGALMVSVVLRPALEPVDAGLLSLLAGAAWAEAIEG
                     "
     gene            407..2137
                     /locus_tag="VIX62_01795"
     CDS             407..2137
                     /locus_tag="VIX62_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5859002.1"
                     /translation="MTTGKPKVLIANRGEIAVRIIRTCRELGIPSVAVYSDVDRDALH
                     VEMADQAFRIGPAQASQSYLSIEAILDAARQAKATMVHPGYGFLSERARFAQDVTDAG
                     LTFVGPSAGAIETMGDKAAARRAADAAGMPIVPGTPEPVDLRSAKRQAERIGFPLLVK
                     AAFGGGGKGMHVVRDAAHLEEALKRAAREAQSYFGRPEVFLERYVDRAHHVEAQIIGD
                     THGNLVFLGERDCSVQRRHQKLIEETPSSVIDEATRTRLAEASIALAREAGYVNAGTV
                     ECIVDEDGDFYFLEMNTRLQVEHTVTEMVTGLDIVALQLEVAMGGTLDIEPHTQGHAI
                     QCRINAEDPGRNFLPGPGRITRYREPAGPFVRVDSGVGEGREVPGDYDSMFAKLIVSA
                     PDRESARRRMLRALDEFVVEGVPTTIPVQRWILDSKEFRSATHTTTWLERALADADLP
                     AQVELQPAAPAPHEGRPADILVEVDGRRVPVRIFDERRDVAPKAPEAHEAHEGGHIHG
                     EIRAPMQGTIVKVLVEKGQEIRAGEVVCILEAMKMENHIPSMRDGEVSDLPIRAGQVV
                     QTGQTLAVID"
BASE COUNT          355 a          764 c          749 g          300 t
ORIGIN      
        1 ccctcgatcg cttcagccca cgcggcgccg gcgagcaggg acagcagtcc ggcgtcgacg
       61 ggctcgagcg cgggacgcag caccaccgag accatgaggg cgcccccggg ccgatccgtc
      121 caggtgcggt cgcgacggcc acgtcccgcc gtctggtgac cggctcccac cagggtccat
      181 tccggaacac cctcgcgggc catgatcgcc gccgtcgcgt tggtggagcc ggtaacctca
      241 tcccaacgca cgggggcggc cagtcctgcc gttcgcgccg cccgttcgag atcgaccgaa
      301 cccatgagcg tttgactccc tcccggagtc cggagagtat cgcgggccag tgaggggcgg
      361 cggaagtgca ggcagccgat gacagcagga cgatgacaac gggccaatga caacgggcaa
      421 accgaaggtc ctgatcgcca accgtggcga gatcgccgtg cggatcatcc gcacgtgccg
      481 tgagctgggc atcccgagcg tcgccgtcta ctccgacgtg gaccgcgacg ccctgcacgt
      541 ggagatggcc gaccaggcgt tccggatcgg tcccgcgcag gcctcgcagt cctacctgtc
      601 gatcgaggcc atcctggacg ccgcccggca ggcgaaggcc acgatggtcc accccgggta
      661 cggattcctg tccgagcgcg cacggttcgc ccaggacgtc accgatgccg gcctcacgtt
      721 cgtcgggcca tcggcgggcg cgatcgagac gatgggcgac aaggcggccg cacgccgggc
      781 cgccgacgcg gcagggatgc cgatcgtccc cggcaccccc gaaccggtcg acctgcggag
      841 cgccaagcgg caggccgaac gcatcgggtt cccgctcctg gtgaaggcgg ccttcggcgg
      901 cggcgggaag gggatgcacg tcgtccggga cgccgcgcat ctcgaggaag cgttgaagcg
      961 cgccgcccgc gaggcgcaga gctacttcgg gcgccccgag gtgttcctgg agcgctacgt
     1021 cgaccgcgct caccatgtcg aagcgcagat catcggtgac acccacggca acctggtgtt
     1081 cctgggcgaa cgcgactgct cggtccagcg gcgacaccag aagctgatcg aagagacccc
     1141 gtcctccgtc atcgacgagg ccacccgcac ccggctggcc gaagcgtcga tcgccttggc
     1201 gcgcgaggcc ggctacgtca acgccggcac ggtcgagtgc atcgtcgacg aagacggcga
     1261 cttctacttc ctggagatga acacccgtct gcaggtggag cacacggtga ccgagatggt
     1321 gaccggcctc gacatcgtcg cgctccagct cgaggtcgcg atgggcggca cgctcgacat
     1381 cgagccccac acccagggcc atgcgatcca gtgtcggatc aacgctgagg atcccggccg
     1441 caacttcctg ccgggccccg gccggatcac ccgctaccgg gagcccgcag ggccgttcgt
     1501 tcgggtggac tcgggcgtgg gtgaaggccg cgaggtcccc ggtgactacg attccatgtt
     1561 cgccaagctg atcgtgtccg cccccgatcg tgagtcggcg aggcgccgga tgctgcgggc
     1621 cctcgacgag ttcgtcgtcg aaggggtgcc caccacgatc ccggtgcagc gttggatcct
     1681 ggattccaag gagttccggt cggccaccca caccaccacg tggctcgagc gcgcgttggc
     1741 cgacgccgat ctgcccgccc aggtcgaact ccaacccgct gcgccggcgc cgcacgaggg
     1801 ccggcccgcc gacatcctgg tcgaggtcga cggtcgccgc gtgcccgtgc gcatcttcga
     1861 cgaacggcgc gatgtcgctc cgaaggcgcc ggaagcccac gaggctcacg aagggggcca
     1921 catccacggc gagatccgcg cgccgatgca gggcacgatc gtgaaggtcc tcgtggagaa
     1981 gggtcaggag atccgcgcgg gcgaggtcgt ctgcatcctc gaggccatga agatggagaa
     2041 ccacatcccg tcgatgcgcg acggcgaggt gagcgacctg ccgatccggg ccggtcaggt
     2101 ggtgcagacg ggtcagaccc tcgcggtcat cgactgatcg ggtccggggt tcggttcccc
     2161 ctcgtcct
//
LOCUS       DATDBZ010000072         4724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_228202, whole genome shotgun sequence.
ACCESSION   DATDBZ010000072 DATDBZ010000000
VERSION     DATDBZ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228202"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2991)
                     /locus_tag="VIX62_01800"
     CDS             complement(<1..2991)
                     /locus_tag="VIX62_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859003.1"
                     /translation="MATLRFLLKRFAAQRLLGLAVIVTLAFSVGVLVAGPIYADAARE
                     AIFSSTLSDAGVTVANARYQVFGDEDFDWANADAAVEGALTGVPTDTIVSQGLSTVRF
                     GDTEGPSLPVFFREGAADHLQIRGEAPAAGEIALPSSTAQTAGLQLGDPVSIVGPNDE
                     TVTLTLSGTYGSPDREDPFWFGSRNPFPGPDATDPPPLLMDREAYLEAATTLGLTSEF
                     AWDAYLAVGDLTYDEARSVPGQLLHAEDVMSEQPGMTTVRLTTGLDTLFGIVDQRVQN
                     LRIPILLVVIQIGAVTLAVLAGVGALALTRQSFELAVLHSRGFTKRTLLTAQGLQALT
                     YAVVAYPLGLLLGLVLAKLAGRSNGKQLPGVVFPVHLNAGAELLGLLAAAAGAAILFG
                     LSYPLVKRTVLEERRAASREDRPLLARVPVELFVLPLGIFAYLQLRGGTEVTPGGGTI
                     DPLVLAAPTLLLFGISFLALRLLLFVLRRLDGRIGRTRRVSTYLAGRRVGRSPGTGFA
                     AALLLLLAMGLLVVSTSYRAIVLRNHADAAHTQVGADWNVQVSPPEQVLAAIGDLPEE
                     ATAVVRTEPTFSDGSFSLPPTVLGIDPATYEEGGWWRSDFSSSSLEDIVEGLATDPLG
                     LPVDAGALTLALDVPVLPQGMLVQATAFDDAGQVRTVEAPLREGSSSYDLDLDAGRLA
                     SITFHVDTGTELPPELKIGFRSVELAGQPLSIEAWEPITWRSSQGTLEPDGDGGPTIY
                     TMSPGAGNVIGGIWPAPPDLPAVISENWGSAVNAPFDVGVAGQSTTVHPFATANQFPS
                     LVPNAPFVVVSAPAILERQFAIPEAGLTLDEVWANTPDDPSPVLENAGFIPGLVQATA
                     PIEGLLAQLPQSLAVGMNFTAALAGVGLVVIGVAAGLYFTMRRRDYEFAALRAMGTRA
                     GQIRRALILEQVGLLGFALVAGIAIGYFLLRLMMPYVGTSLGVPYPVPVLVIDWAALG
                     VAVVAIVVAAAISLAAAMRFLMRSS"
     gene            3134..4294
                     /locus_tag="VIX62_01805"
     CDS             3134..4294
                     /locus_tag="VIX62_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY5859004.1"
                     /translation="MDRPEIILAPGPTPIPPEVLLAQGSPLVYHRGPGFGNLMRDVTT
                     RLKELYKTDSADVLLMTSSGTGGLESAIANCFSPGDEVLVPLAGFFSERFKKLAETYG
                     LTVRTVEYEWGTKIRPDDVAAALAEHPVKAVLLTQSETSTGVIQPIEQLAKVANDAGA
                     LVIVDAVSSLGAVPFEFDAWGIDVAVGGSQKALSASPGIAFCAISERAWEASRSATNP
                     RFYFDWATYKRFADLPHPENPWTPAISVMQGLQAALALYFQDGIEAAMVRHQMLSRAV
                     KEGAKALGLDLFGEGLEDNWTVTAIRAPENIDADTISDTIRADHGCVLAPGQGPLKGK
                     VFRIGHFGYFSELDIIRGLAALEMTLETLGHPVKRGAAVAAAETVFMESRAS"
     gene            4291..>4724
                     /locus_tag="VIX62_01810"
     CDS             4291..>4724
                     /locus_tag="VIX62_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5859005.1"
                     /translation="MRVLVTEQLSEQGLELLRKDFQVDVRTDLAGGDLATEIAPYDAL
                     IIRSATQVTDEVLAAAPQLKVVARAGIGLDNVDVEAATRRGVMVVNAPQSNIISAAEQ
                     AMALLLAQARNTPQANAALKQGAWERSKWEGVELAGKTIGLVG"
BASE COUNT          787 a         1652 c         1639 g          646 t
ORIGIN      
        1 cgacgagcgc atcaggaacc gcatcgcggc ggccagcgag atggcagccg cgacgacgat
       61 cgccaccacc gccacaccga gcgccgccca atcgatcacc agcaccggca cggggtaggg
      121 caccccgagg ctcgtgccga cgtagggcat catcagtcgc aggaggaagt acccgatcgc
      181 gataccggcc accagcgcga accccagcag tcccacctgt tccaggatca gcgcgcgacg
      241 gatctgtccc gctctcgtgc ccatggctcg cagcgcggcg aactcgtagt cgcgccgacg
      301 catcgtgaag tacaaccccg ccgccacgcc gatcaccacg agcccgacgc cggccagggc
      361 agcggtgaag ttcatcccca cggcgagcga ctgcggcagc tgcgccagga gtccttcgat
      421 cggggcggtg gcctggacga ggcccgggat gaagcctgcg ttctccaaca ccggcgacgg
      481 atcgtccggc gtgttggccc acacctcatc gagcgtcagt cccgcttccg ggatcgcgaa
      541 ctgccgctcc aggatcgcgg gcgcggacac gacgacgaac ggcgcgttgg gcacgaggct
      601 ggggaactgg ttggcggtgg cgaacgggtg gaccgtcgtg ctctggcccg ccacgcccac
      661 atcgaagggg gcgttcacgg ccgagcccca gttctccgag atcacggccg gcaggtcggg
      721 cggcgccggc cagatcccgc cgatcacgtt cccggccccc gggctcatcg tgtagatggt
      781 ggggccaccg tcgccgtctg gctcgagggt tccctggctg ctgcgccagg tgatcggttc
      841 ccaggcctcg atcgagagcg gctggcccgc cagttcgacc gaacggaacc cgatcttcag
      901 ttccggaggc agctcggtcc cggtgtcgac gtggaacgtg atcgaagcga ggcggccggc
      961 gtccagatcg aggtcgtagg agctggaacc ctcccgcaac ggagcttcca cggtgcgcac
     1021 ctggccggcg tcgtcgaacg ccgtggcctg gaccagcatg ccctgcggaa ggacggggac
     1081 atcgagggcg agcgtcagcg ctcccgcgtc gaccggcagc ccgagcgggt cggtggccaa
     1141 tccctccacg atgtcctcga gcgatgacga ggagaagtcc gatcgccacc agccgccctc
     1201 ctcgtaggtg gcgggatcga tgcccagcac ggtcggcggc agggagaagc tcccgtcgga
     1261 gaacgtcggt tcggtgcgca ccaccgcggt ggcttcctcg ggaaggtcgc cgatggcggc
     1321 caggacctgt tcggggggac tcacctgcac gttccagtcg gccccgacct gcgtgtgcgc
     1381 ggcgtcggcg tggttgcgca gcacgatcgc gcgataggag gtcgagacca ccagcagccc
     1441 catcgccagc aacagcagga gcgccgcggc gaacccggtg cccggggatc gcccgacccg
     1501 gcgacccgcc aggtaggtgg acacccgtcg cgtccgtccg atccgcccgt cgagccgtcg
     1561 aagcacgaag agcagcaggc gaagcgccag gaacgagatg ccgaacagca gcagggtggg
     1621 ggccgccagg accagcggat cgatcgtgcc cccgcccggc gtcacctcgg tgccgccgcg
     1681 caactggagg taagcgaaga tccccagcgg caggacgaag agctccacgg gcacccgagc
     1741 gagcaacggc cggtcctcac gcgaggccgc ccgccgctcc tccaacacgg tgcgtttcac
     1801 gagcgggtag gagagcccga acaagatcgc ggcccccgcg gccgccgcca gcagaccgag
     1861 cagctcggct ccggcgttga ggtggacggg gaacaccacc ccggggagct gtttgccgtt
     1921 cgagcggccg gcgagcttcg ccagcaccag ccccaaaagc agacccaacg ggtaggccac
     1981 cactgcgtag gtgagcgcct gcaaaccctg tgcggtcagc aacgtccgct tcgtgaaccc
     2041 gcggctgtgc agcacggcga gctcgaagga ctggcgggtg agcgccagcg cccccacccc
     2101 cgccagcacg gccagcgtca ccgcgccgat ctggatcacg acgagcagga tcgggatccg
     2161 aaggttctgc acgcgctgat ccacgatgcc gaacagcgtg tcgagcccgg tcgtgaggcg
     2221 aaccgtggtc atgcccggct gttcgctcat gacatcctcg gcgtggagca gctggccggg
     2281 aaccgagcgc gcctcgtcgt acgtgagatc gccgacggcg aggtaggcgt cccaggcgaa
     2341 ctcgctcgtc agccccagcg tcgtggcggc ctcgaggtag gcctcgcggt ccatgagcag
     2401 cggcgggggg tcggtggcat ctgggcccgg gaacgggttg cgtgagccga accagaacgg
     2461 atcctcgcgg tcgggcgagc cgtacgtccc gctgagcgtc aacgtcaccg tctcgtcgtt
     2521 gggacccacg atcgagaccg ggtcacccag ctgcaggccc gcggtctgcg ccgtgctcga
     2581 gggcagggcg atctcaccgg cggcgggcgc ctctccgcgg atctgcagat ggtcggccgc
     2641 gccctcgcgg aagaacaccg ggagggacgg cccctcggtg tcgccgaacc gcaccgtgga
     2701 cagcccctgg gagacgatcg tgtcggtcgg aacaccggtg agcgccccct ccaccgccgc
     2761 gtcggcgttc gcccagtcga agtcctcgtc accgaacacc tggtagcgcg cgttggccac
     2821 ggtcacgccg gcatcgctca gcgtggagga gaagatggcc tctcgagcgg cgtccgcgta
     2881 gatggggccg gcgaccagga cgcccacgct gaacgccagc gtgacgatga ccgccaggcc
     2941 cagcagacgc tgagccgcga accgcttgag caggaatcga agggtcgcca tcgttggggt
     3001 cggcgcccct ggcgaagcgc ccacaggatg atacggagct gcggttcctt gccgtcgccg
     3061 tccttgcgga atcctgccgt cgacgcattg gtaggctgac ggttccgacg tcgttcgagg
     3121 agtgatccaa ccgatggaca ggcccgagat catcctggcc cccggtccga ccccgatccc
     3181 gcccgaggtg ctgctagccc agggcagccc gctcgtctat caccgtggtc ccggcttcgg
     3241 gaacctgatg cgcgacgtca ccacccggct gaaggagctc tacaagaccg attcggccga
     3301 cgtgctgctc atgacctcca gcggcacggg cgggctcgag tccgcgatcg ccaactgctt
     3361 cagtcccggc gacgaggtcc tggtgcccct ggccgggttc ttctccgagc ggttcaagaa
     3421 gctcgccgag acctacggac tcaccgtgcg taccgtcgag tacgagtggg gcacgaagat
     3481 ccgccccgac gacgtcgcgg ccgcgctggc cgagcatcca gtgaaggccg tgctgctcac
     3541 gcagtccgag acctccaccg gcgtgatcca accgatcgag cagctcgcga aggtcgccaa
     3601 cgacgccggc gccctggtga tcgtcgacgc ggtgtcctcg ctcggcgccg taccgttcga
     3661 gttcgacgcc tgggggatcg acgtcgcggt gggcggcagc cagaaggccc tgagcgcctc
     3721 gcccggcatc gcgttctgcg cgatcagcga gcgcgcgtgg gaagcatcgc gctccgcgac
     3781 gaacccgcgc ttctatttcg actgggccac ctacaagcgg ttcgccgacc tgccgcaccc
     3841 cgagaacccg tggacgcccg cgatcagcgt gatgcagggc cttcaggcgg ccctggcgct
     3901 gtacttccag gacggcatcg aggcggcgat ggttcgccat cagatgctgt cgcgcgcggt
     3961 caaggaaggc gcgaaggcgc tcggcctgga cctgttcggc gaagggctcg aggacaactg
     4021 gaccgtcacc gcgatccgcg cgcccgagaa catcgacgcc gacacgatca gcgacacgat
     4081 ccgtgccgat cacgggtgcg tgctggctcc gggtcaggga ccgctgaagg gcaaggtctt
     4141 ccgcatcggg cacttcggat acttctccga gctcgacatc atccgggggc tcgcggcgtt
     4201 ggagatgacc ttggagacgc ttggtcaccc cgtgaagcgc ggcgctgcgg tggcggccgc
     4261 cgagacggtc ttcatggagt cgcgcgcctc gtgagggtcc tcgtcaccga gcagctctcc
     4321 gagcaagggt tggagctgct ccgcaaggac ttccaggtgg acgtgcgcac cgacctcgcc
     4381 gggggcgacc tcgcgaccga gatcgcgcct tacgacgcgc tcatcatccg cagcgccacg
     4441 caggtcaccg acgaggtgct cgccgccgcg cctcagctga aggtggtggc gcgcgcgggg
     4501 atcggtctgg acaacgtcga tgtcgaagcc gccacgcgcc gcggcgtcat ggtggtcaac
     4561 gcgccgcagt ccaacatcat cagcgccgcc gagcaggcga tggccttgct cctggcgcaa
     4621 gctcgcaaca ccccgcaggc caacgccgcg ctgaaacagg gcgcgtggga gcgctcgaag
     4681 tgggagggcg tggagctcgc cggcaagacg atcggcctcg tcgg
//
LOCUS       DATDBZ010000073         3288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_842521, whole genome shotgun sequence.
ACCESSION   DATDBZ010000073 DATDBZ010000000
VERSION     DATDBZ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842521"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="VIX62_01815"
     CDS             complement(<1..1623)
                     /locus_tag="VIX62_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY5859006.1"
                     /translation="MDLHLMLASPSEDERAAVDDLLGEPTSGWHGAERDEVTHRVARG
                     GRAAREQRDLLLPALHALQSRVGWISQGGLNYVSERLTVPPAEAFGVATFYAMFSVEP
                     RAKIVVHACDDLACRMSGGELVCDALQATFGEPGAAEGEVTWVRSPCLGMCERAPVAL
                     VQTSGEGAQDATFGQVDPRQVQHLVTKTLAGQEFSRPTDPQVSSAPQTWDADGRAALR
                     LLRRVGAVDPASIDDYRAHHGYEALRRAIELGPEETLREITDAKLLGRGGAAFPTGVK
                     WKAVAEQLEHPHFFICNADESEPGTFKDRVVMESDPYAVIEALTIAGVTTGCAQGYVY
                     IRGEYPLATSRLEHALTEARRHGFLGADIMGHGIDFDIEVRRGAGAYICGEETALFNS
                     LEGKRGEPRNKPPFPVQRGLFGKPTGINNVETLINVLEVLRIGSQAYAELGSEGSTGT
                     RLFCVAGSVASPGVYEAEHGITLGEVLALAGGVDGNLKAVLLGGAAGGFVGPDRLDIP
                     LTFEDARAGGYTLGSGVVLAMNQAVDLVDVCLR"
     gene            complement(1711..>3288)
                     /locus_tag="VIX62_01820"
     CDS             complement(1711..>3288)
                     /locus_tag="VIX62_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5859007.1"
                     /translation="RAAHPIFFHHALKGIHNGAQMVVVDPRRTESAEWGDLWLGIDVG
                     SDIGLSNTIAREIIVNDLQHHSFIEGATLGFDGYKDSVMDWTLERGEQVTGVPGEIIR
                     EVAHAYAKADRAMICWTLGITEHHNAVDNVVSLINLALLTGHVGVYGSGVNPLRGQNN
                     VQGGGDMGAIPNKLPGFQDIEQDAEARARFEAAWGVPITPKYGWTLTQMFEAMERKEL
                     TAAYVIGENPASSEADKTHTEELLTGLDCLIVQDLFMTRTAELADVVFPASNAAFESD
                     GTVTNSERRVQRVRKALDPPGSARDDIWIIAQLADRLGAGWGDVTPHEAWEELRALSP
                     MHAGMSWQRIEELGGLQWPCPDEEHPGTLYLHSRLWEFDDPAAQGAKAPFSIVIDEPP
                     VDELSEDFPLRLTTGRRLDSYNTGVQTGGYTSPLRRGETIDVSPEDAERMGLTEEECV
                     LVSSRRGTVEVPVHVDHALRPGLVFMTFHFPDQVDTNVLTIEATDPRSGTAEFKAAAV
                     RIDKMPDAVPVSGGAGE"
BASE COUNT          460 a         1180 c         1116 g          532 t
ORIGIN      
        1 cctcaggcag acatcgacca gatcgaccgc ctggttcatc gccagcacca cgcccgagcc
       61 cagcgtgtac ccgccggccc gcgcgtcctc gaacgtcagt gggatgtcga gacggtccgg
      121 acccacgaaa ccgcctgccg cgccccccag cagcaccgcc ttgaggttgc cgtccacccc
      181 tcccgccagc gcgagcactt cccccagcgt gatgccgtgt tcggcttcgt agaccccggg
      241 gctcgccacc gaacccgcca cacagaacaa ccgggtgccg gtcgatcctt cgctgcccag
      301 ctcggcgtag gcctggctgc cgatgcgcag gacctcgagc acgttgatca aggtctcgac
      361 gttgttgatc cccgtgggct tgccgaagag cccgcgctgc accgggaagg gcggcttgtt
      421 cctcggctcg ccacgtttgc cctcgagcga gttgaacagc gccgtctcct cgccgcagat
      481 gtaggcgccc gcgccccgcc gcacttcgat gtcgaagtcg atgccgtggc ccatgatgtc
      541 ggcgcccagg aacccgtggc gccgggcttc cgtcagggcg tgctccaggc gcgaggtcgc
      601 gagggggtac tcgccgcgga tgtacacgta cccctgcgca cacccggtgg tgaccccggc
      661 gatcgtgagc gcctcgatga ccgcgtaggg gtcggattcc atcacgacgc ggtccttgaa
      721 cgttcccggt tcggactcgt cggcgttgca gatgaagaag tgcggatgct cgagctgctc
      781 ggccacggcc ttccacttga ccccggtggg gaacgcggcg ccgccgcgac ccagcagctt
      841 cgcatcggtg atctctcgga gggtctcctc cggccccagc tcgatcgccc gccgcagcgc
      901 ctcgtacccg tggtgggccc ggtagtcgtc gatcgaggcc gggtcgacag ccccgacgcg
      961 acgcagcagt cggagcgccg cccgtccatc ggcgtcccac gtctgtggcg ccgacgacac
     1021 ctgtgggtcg gtgggacgcg agaactcctg tcccgccagc gtcttggtca ccaggtgctg
     1081 cacctgccgc ggatcgacct ggccgaacgt cgcgtcctgc gcgccctcgc ccgacgtctg
     1141 caccagcgcg acgggcgcgc gttcgcacat gccgagacac gggctgcgca cccaggtgac
     1201 ctcgccctca gcggcacccg gctcgccgaa cgtggcctgc agcgcgtcgc agaccagttc
     1261 gccgccgctc atgcggcacg ccaggtcgtc acaggcgtgg accacgatct tggctcgggg
     1321 ctcgacgctg aacatcgcgt agaaggtggc gacgccgaag gcctcggcgg gcggcaccgt
     1381 gagtcgttca ctgacgtagt tgaggccacc ctggctgatc catcccaccc gggactgcag
     1441 ggcgtgcagg gcgggcagca gcagatcgcg ctgctcgcga gccgcgcggc ctccccgggc
     1501 cacgcggtgc gtgacctcgt cgcgctcggc gccgtgccag ccgctggtgg gttcgcccag
     1561 gagatcgtcg accgctgcgc gctcgtcctc cgagggcgag gcgagcatca ggtgcaggtc
     1621 cacggcacca ccttcgaggt ctccgacctg gctgcgaggg atgccggcga agcgtcgatg
     1681 tccaaggcga ggggcgtcac ggccgggatg ctactccccg gccccgcccg agaccggaac
     1741 ggcatcgggc atcttgtcga tccgcaccgc cgccgccttg aactccgccg tgccagatcg
     1801 ggggtcggtg gcttcgatcg tgagcacgtt cgtgtcgacc tggtcgggga agtggaacgt
     1861 catgaacacg agccccggcc gcagcgcgtg gtcgacgtgc accggcacct ccaccgtgcc
     1921 gcgacggctc gagaccagca cgcactcctc ttcggtcagc cccatccgct cggcatcctc
     1981 tggcgagacg tcgatcgtct cgccgcgtcg cagcggcgag gtgtagccgc cggtctgcac
     2041 gccggtgttg taggagtcca accgccgccc cgtggtgagc cgcagcggga agtcctcgga
     2101 caactcgtcc acgggaggct cgtcgatcac gatcgagaac ggcgccttcg cgccctgggc
     2161 cgcgggatcg tcgaactccc agagccggga gtgcaggtac agcgtgcccg ggtgttcctc
     2221 gtccgggcag ggccactgga ggccacccag ttcctcgatg cgctgccagc tcatgccggc
     2281 gtgcatcggg ctcagggcgc ggagctcctc ccacgcttcg tgcggggtga cgtcgcccca
     2341 gccggcgccg agccggtcgg cgagctgggc gatgatccag atgtcgtcgc gcgcgctgcc
     2401 gggcggatcc agggccttgc gcacccgctg cacgcgtcgc tcgctgttgg tcacggtgcc
     2461 gtcggactcg aaggccgcgt tggaggccgg gaacaccacg tcggcgagct cggcggtcct
     2521 ggtcatgaac agatcctgca cgatcagaca gtccagcccg gtgagcagct cctcggtgtg
     2581 ggtcttgtcg gcctcgctcg aggccgggtt ctcgccgatg acgtaggcgg ccgtgagttc
     2641 cttccgctcc atcgcctcga acatctgggt gagggtccag ccgtacttcg gcgtgatcgg
     2701 cacgccccag gcggcctcga accgcgcccg cgcctcggcg tcctgctcga tgtcctggaa
     2761 gcccgggagc ttgttcggga tggcgcccat gtcgccgccc ccctgcacgt tgttctggcc
     2821 gcggaggggg ttcacgccgc tgccgtagac ccccacgtgg ccggtgagca gcgcgagatt
     2881 gatcagcgag accacgttgt ccacggcgtt gtggtgctcg gtgatgccca gggtccaaca
     2941 gatcatggcg cgatcggcct tggcgtaggc gtgcgcgacc tcgcggatga tctcgcccgg
     3001 cacgcccgtg acctgctcgc cgcgctcgag ggtccagtcc atgaccgaat ccttgtatcc
     3061 gtcgaacccg agcgtcgccc cttcgatgaa gctgtggtgc tggaggtcgt tgacgatgat
     3121 ctcgcgcgcg atcgtgttgc tcagcccgat gtcggagccg acgtcgatgc ccagccacag
     3181 gtcgccccac tcggccgact cggtgcgccg ggggtcgacc accaccatct gggcaccgtt
     3241 gtggatgccc ttgagggcgt ggtggaagaa gatcggatgc gccgctcg
//
LOCUS       DATDBZ010000074         2031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1865888, whole genome shotgun sequence.
ACCESSION   DATDBZ010000074 DATDBZ010000000
VERSION     DATDBZ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865888"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VIX62_01825"
     CDS             complement(<1..634)
                     /locus_tag="VIX62_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5859008.1"
                     /translation="MRRVTARVRSWGLGVGGTLALAWLALAGAAAIVAPWLPFQRRQD
                     LSNIRAAPSWAHPFGTDSRGIDMVGRVLDGARISLLIGLVSSLLGLLVGATIGIIAGY
                     RRGQADRAIVVGLDVFAAFPAFVAASLASLVWGQSLTNLMFVLGVLTAPIFARVTRAA
                     TLPLAERDFVLASRMIGARHRRVMVRELLPNIAVPLLSYALLAVGIVIAIE"
     gene            complement(631..1590)
                     /locus_tag="VIX62_01830"
     CDS             complement(631..1590)
                     /locus_tag="VIX62_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859009.1"
                     /translation="MTWGWLWRRLVSLAVVLLAVSMATYLLFELLPGRTSTAILGPGA
                     TPEALATVERDLRLDEPLPVRFGIWLGRAVQGDLGVSYRTGEVVTVTLRDRIPTSLEL
                     LVLTQLTAVGIAGVAALVSARREGSRVDRVLSALSFVAIALPQFAFGIVLLIVFAQKL
                     DWFPVAQYSRFSDGPADNLRALVLPVVTLALPLAGIYYRVLRTDLLQTLRSDHITFAR
                     AMGMSPRRVLVGRALRPSSLTLISVIGINTAFLLGGAVIVERLFSLPGLGQLITVSVA
                     SRDIVMVQGATLSIALVYVVVNLVVDVMLTVLDPRVRLVRSAS"
     gene            complement(1600..>2031)
                     /locus_tag="VIX62_01835"
     CDS             complement(1600..>2031)
                     /locus_tag="VIX62_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859010.1"
                     /translation="IEDAVRATVGYWNDAGIDAELQLVPDLGTYITDVLTGNYDVLAY
                     IGGSVGDPDSIVYNLFHTGGATNYGKYSNPEMDAALESGRSSNDDATRKADYTAVQQI
                     VREDVPVLLTSHGQIYIVASDRVGGLDPSFFFPSRTVSLAA"
BASE COUNT          385 a          675 c          716 g          255 t
ORIGIN      
        1 cctcgatcgc gatcacgatg ccaacggcga gcaatgcgta agagaggagc ggcacggcga
       61 tgttcggcag aagctccctc accatcacac gacgatggcg ggcgccgatc atcctcgacg
      121 ccaggacgaa gtcgcgctcg gccagcggca gcgtcgcggc gcgggtgacg cgggcgaaga
      181 tcggcgcggt gaggacgccg aggacgaaca tcaggttggt gaggctctgg ccccacacga
      241 gcgacgccag ggacgccgcg acgaacgccg gaaaggcggc gaacacatcg agaccgacga
      301 cgattgcacg atccgcctgg ccccgccggt agcccgcgat gatgccgatc gtggccccga
      361 cgagcagccc gagcagcgag gacaccaagc cgatgagcaa cgagatgcgg gcaccgtcca
      421 gcacccggcc gaccatgtcg atgccgcgcg agtcggtgcc gaacggatgg gcccagctcg
      481 gagcggcgcg aatgttcgag aggtcctgac ggcgctggaa gggcagccag ggcgccacga
      541 ttgcggccgc cccagcgagc gcgagccatg cgagggcgag cgtcccaccg acgccgaggc
      601 cccacgagcg cacccgcgcc gtgacgcgcc tcacgatgcg ctccggacga gccgcacgcg
      661 cgggtcgagc accgtcagca tgacgtccac gacgaggttc acgaccacgt acaccagcgc
      721 gatggagagc gtggcgccct gcaccatcac gatgtcccgc gacgccaccg acacggtgat
      781 cagctgaccg agccccggta gcgagaacag tcgttcgacg atcacggctc ccccgagcag
      841 gaacgcggtg ttgatcccga tcaccgagat cagcgtgagc gaactgggtc gcagcgcgcg
      901 accgacgagg acccgtctgg gcgacatccc catggcgcgg gcgaaggtga tgtggtcgct
      961 ccgcaaggtc tgcagcaggt cggtgcgcaa cacgcggtag tagatgccgg ccagcgggag
     1021 cgcgagcgtc accaccggca gcaccaacgc acggaggttg tcggccgggc cgtccgagaa
     1081 gcggctgtat tgcgccaccg ggaaccagtc gagcttctgg gcgaacacga tcaacagcac
     1141 gatgccgaac gcgaactgcg gtagggcgat ggcgacgaac gacaacgccg agagcactcg
     1201 gtccacacgg ctgccttcgc ggcgggccga gacgagtgcg gcgacgccgg cgatgcccac
     1261 tgcggtcaac tgggtcagca cgagcagttc gagcgacgtc gggatgcggt cgcgcaacgt
     1321 gacggtgacc acctcgccgg tccggtagga cacgccgagg tcaccctgca ccgcccggcc
     1381 gagccagatg ccgaagcgca ccggcagggg ttcgtcgagc cgaagatcgc gctccaccgt
     1441 ggcgagcgct tcaggggtcg ctcccggccc gaggatggcg gtcgatgtgc gtccgggcag
     1501 cagctcgaac aacaggtacg tcgccatcga cacggcgagg aggaccacgg ccagcgagac
     1561 cagacggcgc cacaaccacc cccacgtcat gggatgaggt caggcagcga gcgacacggt
     1621 gcgcgacggg aagaagaagc tggggtcgag cccaccgact cggtccgacg ccacgatgta
     1681 gatctggcca tgtgacgtca ggagcaccgg cacgtcttcg cgcacgatct gctgcacggc
     1741 ggtgtagtcg gccttccgtg tggcgtcatc gttgctgctg cggccgctct cgagcgcagc
     1801 atccatctcg gggttgctgt acttgccgta gttcgtcgcg ccgcccgtgt ggaagaggtt
     1861 gtagacgatg ctgtccggat cgccgaccga accgccgatg taggcgagca cgtcgtagtt
     1921 cccggtcagg acgtcggtga tgtacgtccc gaggtcgggc acgagttgca gctcggcgtc
     1981 gatcccggcg tcgttccagt agccgaccgt ggcccggacg gcgtcctcga t
//
LOCUS       DATDBZ010000075         2892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_432156, whole genome shotgun sequence.
ACCESSION   DATDBZ010000075 DATDBZ010000000
VERSION     DATDBZ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432156"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2412)
                     /locus_tag="VIX62_01840"
     CDS             complement(<1..2412)
                     /locus_tag="VIX62_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5859011.1"
                     /translation="MLATWITRQLVFDGVITGLLYALLAMGIVLIYRATRVINFAVGA
                     MGLVGGALFALLVVEWNVPFWIGAVVALGVGILYGAILELLIIRRLFAAPRVIVLVAT
                     VGIAQLSQLIVLAYPEFSRSGELFPRPIGTTYEGFLGLRIQGPQLAILIVAPIVSIAV
                     GLFLTRTTLGKTVKASSSNRDLARLVGISPKMVSTFVWALGGALATVAMCLMAMQTNA
                     RVGGLALLGPSTLVRALVAAVVAKLTSFQRAFVAAVVIGVVETVLRFNYLDQPGLMDF
                     LLLIVVLVAVFFQSRTREPGETQTFSFAPRVKPVPERLRSIWWVRQLDRAPLVLIVLG
                     AVLIPLIITQPSRHLLYGTILAYAIIATSLNILTGWAGQLSLGQMAFAGIGAFLAAAF
                     SRGINWDIKIGDTRLYKAGFEGMPFGLSIVLAALVTAAVATIIGVGALRVRGLLLAVV
                     TFAFAHASTQYLFRQRILTGGLTSRIPFPRTGIFGIDLSSQRSIYYLCLAVLSLVLVM
                     VGWLRRTAVGWSTIAVRDNPDTASAYTVRPPIVKLRIFALAGGMAGLGGALLAAVTEG
                     FPVPVARYFELDDSLILLSIVVIGGLGSTAGPVLGALWVVGLPAFFPNNPVAPLLTSS
                     VGMLILLMYFPGGLVQVGYAARDTLLGWLDRRMDQVPVKTRHVVPMSLAIDREARSAG
                     AGPALRTEALTVRYGGRLAVDAVSLEVGRGEIVGLIGTNGAGKSSLMNAIGGFVSADG
                     DVQLLGARVSGKSPAGRARLGLGRTFQAATLFPELTVRETVEVAMEGRGRSGMVATAL
                     AWPPTL"
     gene            complement(2415..>2892)
                     /locus_tag="VIX62_01845"
     CDS             complement(2415..>2892)
                     /locus_tag="VIX62_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5859012.1"
                     /translation="PGGKGVFPDMTVADNLEMGAFVYRDDAADVARRIAHVYELFDDL
                     ASRKEQIAGSLSGGQEQMLALAMTLLHEPDVLLIDELSLGLAPVVVQDLMHVVETLKG
                     RGMTIVIVEQSLNIALEISDRAVFLEKGQIRVEGPSAELAARDDLVRAVFLGHEGG"
BASE COUNT          578 a          962 c          941 g          411 t
ORIGIN      
        1 cagcgtcggg ggccacgcga gagcggtcgc caccattcca gaccgcccgc gcccctccat
       61 cgccacttcg acggtttcac gcaccgtcag ttccgggaac aacgtcgccg cctggaacgt
      121 ccggccgagg ccgagacgag cacgccccgc cggtgacttg cctgacaccc tcgcgcccag
      181 gagctggacg tctccgtcgg cgctgacgaa cccaccgatc gcgttcatca agctcgactt
      241 gcccgcgccg ttcgtgccga tcaggccgac gatctcccca cgacccacct cgagcgagac
      301 ggcgtcgaca gcgagccggc cgccgtaacg gacggtgagc gcctcggttc gcaacgcagg
      361 gccggcgccc gcggagcgcg cctcccgatc gatggccagc gacatcggaa cgacatggcg
      421 cgtcttgacc gggacttgat ccattcttcg gtcgagccag ccgaggagcg tgtcgcgtgc
      481 cgcgtacccg acttggacga ggccgccggg aaagtacatc aggaggatga gcattcccac
      541 gctcgaggtg agcagtggtg ccaccgggtt gttcgggaag aaggcgggca gtccaaccac
      601 ccagagcgcg ccgagtaccg ggccggcggt ggagccgagg ccgccgatga ccacgatcga
      661 caggaggatg agcgagtcgt cgagttcgaa gtagcgcgcc acgggaaccg ggaagccctc
      721 ggtgaccgcc gccagcagcg ctcctccgag tccggccatg ccaccggcca acgcgaagat
      781 cctgagcttc acgatcgggg ggcggacggt gtaggccgac gcggtgtctg ggttgtctcg
      841 aaccgcaatg gtcgaccacc ccaccgcggt gcggcggagc caaccgacca tcacgagcac
      901 cagcgacaac accgccaggc agaggtagta gatcgagcgt tgcgacgaga gatcgatccc
      961 gaagatgccg gtccgcggga acggaatgcg actggtcaga ccgccggtga ggatgcgctg
     1021 ccggaacagg tactgcgtgg acgcatgggc gaaggcgaat gtgaccacgg cgagcaacaa
     1081 cccgcgcaca cggagcgcgc cgacaccgat gatcgttgcg actgcagccg tcaccagagc
     1141 ggcgagcacg atcgagaggc cgaacggcat cccttcgaaa ccggccttgt acagcctcgt
     1201 gtctccgatc ttgatgtccc agttgatgcc cctgctgaag gcggcggcaa ggaacgcgcc
     1261 gatgcctgca aaggccatct ggccaagtga cagctgcccc gcccaccccg tcaggatgtt
     1321 gagcgatgtc gcaatgatcg cgtaggcgag gatcgttccg tagagcaggt gacgcgaggg
     1381 ttgcgtgatg atgagcggga tgagcaccgc acccaacacg atgagcacga gcggagcgcg
     1441 atcgagctgg cgaacccacc agatgcttcg gagtcgttcg gggaccggct tgacgcgagg
     1501 cgcgaacgag aacgtctggg tctctccggg ttcacgtgtc cgtgactgga agaacaccgc
     1561 aacgagcacc acgatcaaca gcaggaagtc catcagcccg ggctgatcga gataattgaa
     1621 gcgcaacacc gtctcgacga cgccgatcac caccgcggcc acgaaggccc gctggaacga
     1681 ggtcaatttc gccaccaccg cggcgacgag cgcgcgcacg agggtgctcg gcccgagcaa
     1741 tgccagcccg ccaacccgtg cgttcgtctg catcgccatc agacacatcg ccacggtggc
     1801 gagcgcgcca ccgagcgccc agacgaacgt cgaaaccatt ttcgggctga tgcccaccag
     1861 gcgcgccaga tcgcggttgc tgctcgacgc cttgacggtc ttgcccaagg ttgtcctcgt
     1921 caggaacagg ccaacggcga tcgacacgat tggagcgacg atcaggatgg ccagttgcgg
     1981 gccctggatc cgcaggccga ggaagccttc atacgtggtc ccgatcgggc gcgggaacag
     2041 ttcgccggat cgactgaact ccgggtaggc cagcacgatc agctgtgaga gctgggcgat
     2101 tccgacggtc gcgacgagca cgatgacgcg cggtgcggcg aacagccggc gaatgatgag
     2161 caattcgagg atcgccccgt acagaattcc gacgccgagc gcgacaacgg cgccgatcca
     2221 gaacgggacg ttccactcga ccacgagcag cgcgaacagc gccccgccga ccaagcccat
     2281 ggcaccgacg gcaaagttga tcacccgggt cgcccgatag atcaacacga tgcccatcgc
     2341 cagcagggcg tagagcaatc cggtgatcac cccgtcgaag acgagctgtc gagtgatcca
     2401 cgtggcgagc atcctcagcc gccctcgtgg cccaggaaca cggcgcgcac gaggtcgtcg
     2461 cgtgctgcca gttcggccga gggcccttcg acgcggatct gccccttttc caggaagacc
     2521 gcgcgatcgg agatctcgag ggcgatgttc agcgactgtt ccacgatgac gatggtcatc
     2581 ccacggcctt tgagcgtctc gacgacgtgc atcaggtctt gcaccacgac cggcgcaagt
     2641 ccgagtgaca gttcatcgat cagcagaacg tcgggctcgt ggagcagggt catcgccagg
     2701 gcgagcatct gttcctggcc gcccgacaag gatcccgcga tctgctcctt tcgggaggcg
     2761 agatcgtcga acaattcgta cacatgggcg atccgtcgcg ccacatcggc ggcgtcgtcc
     2821 cggtagacga atgcccccat ctcgaggttg tcggccaccg tcatgtccgg gaacacgccc
     2881 ttgccgccgg gc
//
LOCUS       DATDBZ010000076         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_230437, whole genome shotgun sequence.
ACCESSION   DATDBZ010000076 DATDBZ010000000
VERSION     DATDBZ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230437"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VIX62_01850"
     CDS             complement(<1..224)
                     /locus_tag="VIX62_01850"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY5859013.1"
                     /translation="MDDQVPFEDFFKAEQHRLFGALCLLTHDRIEAEEIGQEAFVRVW
                     ERWDRVGVMADPTGYLYRVAMNLFRSRYRRA"
     gene            418..1320
                     /locus_tag="VIX62_01855"
     CDS             418..1320
                     /locus_tag="VIX62_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859014.1"
                     /translation="MDDHPGPQAATPSPPAMPPAPQPPSRRGPWAIVLAILATIVAAG
                     IIVAIVSRSGSDGSADEPSPSSPSSDVEVAAPSDLEAQPSAFRVVLTWDRSEGTAPDG
                     YTVYRDGETIGIAEGSEHRFVDSGAMPSSTYRYLVKAYAGELGIESKGTGTSVHTPDA
                     PMAYARLQGVYEVRLSVSSSYGLNDVKAYTGAWRFTPICKTGPCRVRFGDLNGPIPNM
                     ELDQRSAVYDGQVSGPGGWECGGVSSTATLHLHLHLDEAGTVNDQWRARKISGTYSVS
                     ISPQLGCRAGGATYAVHGVLHTGP"
     gene            complement(1324..1995)
                     /locus_tag="VIX62_01860"
     CDS             complement(1324..1995)
                     /locus_tag="VIX62_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein"
                     /protein_id="HEY5859015.1"
                     /translation="MKDAPLLRGPLAIRVVVLFVGLVIVAVAIVCMLESELGLPPWDV
                     LHQGISEHTPLSLGNAAIVVGLVIVAVSWIAGVPPGFGTIANAIVIGAAIDILVAIPW
                     VDSLSGTSLSVRIGLVAAGILLFGVGSAFYIGAGLGAGPRDSMMLVLTRRTGWRIGIV
                     RAAIEIAVLIAGLLLGGTAGIGTLALALLVGPSVELSFWALVKLGLAAPGAKPAPEFG
                     PLDVG"
BASE COUNT          391 a          758 c          694 g          310 t
ORIGIN      
        1 gctcgccgat atcggctgcg gaacaggttc atcgcgacgc ggtagagata gcccgtagga
       61 tcggccatca caccgacacg atcccagcgt tcccagacgc ggacgaaggc ctcctggccg
      121 atctcctcgg cttcgatccg gtcgtgggtg agcaggcaga gcgcaccgaa cagccgatgc
      181 tgctccgcct tgaagaagtc ctcgaacgga acctgatcgt ccacagcggt gcgcgccctt
      241 gccctcggcc gtccaacaac tcatcaccgc tcgacgatcc atgacgaacc atgaacctcg
      301 ggcacgcgcg gcgtgcttac cgtgcgccag aagtttcgca cctggggaac cgagagggaa
      361 atcgtgtaag cagttcggct ccctcacggc ttcatgccac cgaccaggag gcgcgagttg
      421 gacgatcatc ccggaccgca ggccgcaacc ccctcgccgc cagcgatgcc gccggcgccg
      481 caaccgccgt cacggcgagg accgtgggcc atcgttctcg cgatcctcgc cacgatcgtg
      541 gcggcaggca tcatcgtcgc gatcgtgtcg agatcgggct cggacgggtc ggctgatgaa
      601 cccagccctt cctccccctc ttcagacgtc gaggttgccg ctccgagtga cctggaggct
      661 cagccctccg ccttccgcgt ggtgctcacg tgggatcgtt ccgaagggac ggcgccggac
      721 gggtacacgg tctaccgcga tggcgaaacc atcgggatcg cggaggggtc ggaacatcga
      781 ttcgtggact cgggggccat gccttccagc acctaccgct atctcgtgaa ggcctatgcg
      841 ggggagctgg ggatcgagtc gaaggggacg ggtacgtcgg tgcatacccc cgacgcaccg
      901 atggcctacg ctcgactcca gggcgtgtac gaggtccgcc tctccgtgag ctcgtcgtac
      961 gggttgaacg atgtcaaggc ctacaccggg gcctggcggt tcaccccgat ctgcaagacc
     1021 ggtccctgcc gggttcggtt cggtgatctc aacggaccga tccccaacat ggagctggac
     1081 caacgttccg cggtgtatga cgggcaggtc tcgggccccg gcggatggga gtgcggcggc
     1141 gtatcgtcca ccgccacact gcatctccat ctgcatctcg acgaggccgg caccgtgaac
     1201 gatcagtggc gggccaggaa gatctccggt acgtacagcg tttcgatctc cccgcagctg
     1261 gggtgccgcg ccggcggcgc gacctacgcc gtccatgggg tcctgcatac aggaccctga
     1321 tcgtcagccg acgtcgagcg gaccgaactc gggcgcgggc ttcgcgcccg gtgcggcgag
     1381 gccgagcttc accagcgccc agaacgacag ttcgaccgac ggccccacga gcagggcgag
     1441 cgccagggtc ccgatgccgg cggtgccacc gagcagcaga ccggcgatca acacggcgat
     1501 ctcgatcgct gcccgcacga tcccgatccg ccagccggtc cggcgcgtca gcaccagcat
     1561 catcgaatcc cgcgggcccg cgcccaggcc cgctccgatg tagaaggccg atcccacgcc
     1621 gaacagcaag atcccggcgg cgacgaggcc gatccgcacg gacagcgacg tccccgacaa
     1681 cgagtccacc cacgggatgg cgaccaggat gtcgatcgcg gcgccgatca cgatcgcgtt
     1741 ggcgatcgtg ccgaaccccg gcgggacacc ggcgatccac gacaccgcca cgatcaccag
     1801 gccgacgacg atggccgcat tccccaggga gagcggggtg tgctcggaga tcccttggtg
     1861 cagcacgtcc cacggcggca accccagctc ggattcgagc atgcagacga tcgccaccgc
     1921 gacgatcacg aggccgacga acagcaccac cacgcggatc gccaagggcc cgcgcagcag
     1981 cggcgcgtcc ttcatcaggg gtgctcccgg cgaccggaga tcatcgaacg agactacgcg
     2041 tcgcgtgccc gactacactg cgacccagcc gacaggagga tccatggccg agaccgtcac
     2101 gatcgaagga cagcccttcc tcaagcgtca gccgctgggg gtgctgggcc tgt
//
LOCUS       DATDBZ010000077         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_640836, whole genome shotgun sequence.
ACCESSION   DATDBZ010000077 DATDBZ010000000
VERSION     DATDBZ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640836"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VIX62_01865"
     CDS             <1..356
                     /locus_tag="VIX62_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEY5859016.1"
                     /translation="ARGDAAARSIIDRQADEVVALATTAIRRLRMTRLDVDVVLGGGI
                     FRNDDAVFFDRIRDGVAQVAPAATITVLSDPPVIGAAQLGLDRVGATRAAHVRARAAL
                     THERLAAHTHRAQKE"
     gene            363..1562
                     /gene="ugpC"
                     /locus_tag="VIX62_01870"
     CDS             363..1562
                     /gene="ugpC"
                     /locus_tag="VIX62_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017608447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY5859017.1"
                     /translation="MAKIVLDQVSKVFGNEVIAVNDVSLEIGDGEFMVLVGPSGCGKS
                     TILRILAGLEEVTAGEVFIGDTQVTDLMPKDRDIAMVFQNYALYPHMSVEQNLGFGLK
                     LRKTPKAEQQQRVQDAARILGLDPLMQRKPAELSGGQRQRVAIGRAMVREPRAFLMDE
                     PLSNLDAKLRVQMRAELSRLHERLGTTTVYVTHDQVEAMTLGHRVAVLKDGVLQQVDT
                     PQHLYRYPANLFVAAFIGSPPMNLVEATVDGSSLAFAEQRIEIGADAGLAGFEGRSVI
                     LGIRPSDLQDMDVWRNDDLPVIEVTAEVTEELGSEVNIIFPIDAPPVLTEDTIAAASD
                     EGDADTIPLIADTQERCRFCARVDSRTSARPGSRVRLSLDPSRFHFFDPETGLAVGTR
                     AAATVGA"
     gene            complement(1574..>2144)
                     /locus_tag="VIX62_01875"
     CDS             complement(1574..>2144)
                     /locus_tag="VIX62_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY5859018.1"
                     /translation="LRMGMMVERDEPVDPTLIATLQETVRQSIERLRSLLFELRPAAL
                     DRDGLVAALTQYLQHTSKETGWTFEIVDRLEFEPDPELRATLYRTVQEAVANARKHSS
                     ASRVVVTVITEGDGITIRVSDDGGGFDTGRLDEPRPGHLGLVTMVERAELMGGWCRVS
                     SVPSEGTTVECWLPSDGGTALDLRPGRSA"
BASE COUNT          353 a          772 c          706 g          313 t
ORIGIN      
        1 cggcccgggg cgatgccgcc gcgcgctcga tcatcgaccg gcaagccgat gaggtcgtgg
       61 ccctggcgac caccgcgatc cgccggctcc gcatgacccg gctcgatgtc gatgtggtgc
      121 tgggcggcgg gatcttccgc aacgacgacg cggtcttctt cgaccgtatc cgagacggcg
      181 tcgcccaggt ggcccccgcg gcgacgatca ccgtcctcag cgatcctccg gtcatcgggg
      241 ccgctcaact gggactcgac cgcgtcggcg ccacgcgagc ggcgcacgtc cgagcccggg
      301 ctgccttgac ccatgagcgg ctcgccgccc acactcaccg cgcacagaag gagtgacgca
      361 ccatggcgaa gatcgtcctc gatcaggtct ccaaggtctt cggcaacgag gtgatcgccg
      421 tcaacgacgt ctccttggag atcggcgacg gcgagttcat ggtcctggtc ggaccatctg
      481 gctgcgggaa gtccacgatc ctgcggatcc tcgccggttt ggaggaggtc accgccggtg
      541 aggtcttcat cggcgacacg caggtcaccg acctgatgcc caaggaccgg gacatcgcga
      601 tggtgttcca gaactacgcc ctctaccccc acatgagcgt cgaacagaac ctggggttcg
      661 gcctgaagct ccgcaagaca cccaaggccg agcagcagca acgcgtgcag gacgccgccc
      721 ggatcctcgg actcgatcct ctgatgcaac gcaagcccgc agagctgtcg ggcggccagc
      781 gacagcgcgt cgccatcggc cgcgccatgg tgcgcgagcc gcgcgcgttc ctcatggacg
      841 agcccctgtc gaacctggac gcgaaactgc gggtgcagat gcgcgccgaa ctctcccgac
      901 tgcacgaacg gctcggcacc accacggtct acgtcaccca cgaccaggtc gaggcgatga
      961 cgctgggcca ccgcgtcgcc gtactcaagg acggcgtgct gcagcaggtg gacacgcccc
     1021 agcacctcta ccggtatccc gccaacctgt tcgtcgcggc gttcatcggc tcgccgccca
     1081 tgaacctggt ggaggccacg gtcgatggct ccagcctggc gttcgccgag cagcggatcg
     1141 agatcggcgc cgacgcgggc ctcgccgggt tcgagggacg ctccgtgatc ctgggcatcc
     1201 gcccgtccga cctgcaggac atggacgtgt ggcgcaacga cgaccttccc gtgatcgagg
     1261 tcacggcaga ggtcaccgag gaactgggct cggaggtgaa catcatcttc ccgatcgacg
     1321 cgccgcccgt cctgaccgag gacacgatcg cggcggccag cgatgagggc gacgccgaca
     1381 cgatcccgtt gatcgccgat acgcaggaac gctgccggtt ctgcgcccgg gtcgactcgc
     1441 ggacctcagc gaggccgggg agccgggtgc gcctctcgct cgaccccagc cggttccact
     1501 tcttcgaccc ggagaccggc ctggccgtcg ggacacgcgc ggcggccacc gtcggggcct
     1561 gaggccctgg acttcaggcc gagcgaccgg ggcgcaggtc caaagcggtc ccaccgtcgg
     1621 acgggagcca gcactccacg gtcgtgccct ccgagggcac gctcgagacg cggcaccagc
     1681 cgcccatcag ctcggcccgc tccaccatgg tcaccagccc caggtgcccg ggccggggtt
     1741 cgtccagccg cccggtgtcg aaaccaccgc catcgtcgga gacccggatc gtgatgccgt
     1801 caccctccgt gatcaccgtg accaccacgc gcgaggcgga cgagtgcttc cgggcgttgg
     1861 ccaccgcctc ctgcaccgtg cggtagagcg tggcccggag ctcgggatcg ggctcgaact
     1921 cgagacgatc cacgatctcg aacgtccacc ccgtttcctt cgaggtgtgc tgcaggtact
     1981 gcgtgagggc ggccaccagc ccgtcacggt cgagcgccgc cggacggagt tcgaacaaca
     2041 atgaacggag ccgttcgatc gattggcgca ccgtctcctg cagggtggcg atgagggtgg
     2101 gatccaccgg ttcgtcgcgc tccaccatca tgcccatgcg gagg
//
LOCUS       DATDBZ010000078         6072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1457496, whole genome shotgun sequence.
ACCESSION   DATDBZ010000078 DATDBZ010000000
VERSION     DATDBZ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457496"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VIX62_01880"
     CDS             complement(<1..470)
                     /locus_tag="VIX62_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859019.1"
                     /translation="MGARQDVVVMGAGHNGLVAAAYLAKGGKKVTVLEAAPEVGGVLR
                     NSTVAEGFTAPGITHTIGRLRPTVVKDLKLEQHGLQTIAPEVRLFAPQPDGSAVTFWG
                     DPGRTATELRERNAHDGDVFVEFDQKMRAIASFLAYINVATPPDASSPSIADAF"
     gene            complement(502..2103)
                     /locus_tag="VIX62_01885"
     CDS             complement(502..2103)
                     /locus_tag="VIX62_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5859020.1"
                     /translation="MAKKYDAVVIGGGHNGLVNAAYLAKAGLKVLVLERRHILGGATL
                     TEEIVPGFKFSVFSYVVSLLRPEIIRELELAKHGLEILPLDGTITPLEDDYLWRVNDH
                     GKTIRELRRWSLSDAEVYEEYGSLMAEMGRFIKPILSIAPPDPGKISPSHWLPLGPLA
                     KAFKDLPKKLQTTFIQLMTMSAADFLDQWFETEPLKATMSASGIIGTFQGPRSPGTAY
                     VLLHHYMGEIDGAFRAWGVPRGGTGSVAYAIANSALSLGVEIRTEAGVDHVKTAGGRA
                     TGVVLSSGEEIDADVVMSSLDSRQTFINLLDESDLDPTFRDEVMRFKYRGSSGKVNLA
                     LSGLPELACKPGVGEWLRGAISFSPSLDYMERAYDDAKYGRPSKRPYIDCIMPTLVDP
                     TMAPPGKHVMSCFVQYAPYHLENGEQWDDTKREAFGQNVIDTLEERFPNIRNLIEGHQ
                     FVTPKDIEDITGLSEGNIFAGELSLEQLFFNRPVPGWARYETPVRDLWMCGSATHPGG
                     GIMGAPGMIAAREYLKQRKRGRRAA"
     gene            2345..3538
                     /locus_tag="VIX62_01890"
     CDS             2345..3538
                     /locus_tag="VIX62_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY5859021.1"
                     /translation="MSLGTAFFEREQALNKKLAWGEWSGYFSAPVYADFHDIEYNAIR
                     EAAGVIDITPLYKYEVSGPDAVRLLDRVMTRDMTKLRIDRIFYSPWCDEEGKVLDDGT
                     ITRLAEHRFRITAADPCYRWFVLNATGLDVEVADVSDATAALALQGRLSREVLQDATG
                     LDWADLRYFGRRGTEIAGVPVDVTRTGYTGDRGYELWMAKDDAVTVWDRIFEVGQRYG
                     IHPVGIRAMDVARVEAGLILIEAEYTSARHAMAPEQQYSPFELNFDGMVDFAKASDFN
                     GRRALQAEQKAGGPERRLVGLHLDWSGIEGMYAKHGLAPAISPYVHRDPIPVYKEGKQ
                     VGRTTSTTWGPTIKKMVSFGPVDKGHSVLGSRLSVEWTVEGERGKVGATVVPMPFFDP
                     ERKRS"
     gene            3611..5524
                     /locus_tag="VIX62_01895"
     CDS             3611..5524
                     /locus_tag="VIX62_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5859022.1"
                     /translation="MRPSRASMLLAAVGGLVGFLVGAAGSLILGLAAGLITFLICLWV
                     YARVTREDRAPSDDGAADPGRRRFLVGVGVGGFALALAGAPIGWLARKLSRPDPAPIQ
                     TAMAEGLGSEYMEFVRRAYRPGRSGDIQMLMAPYNSANYANESLSLVPQDPRTSHASL
                     WMYLERIPMVVYGPGIVASSDSTERVTLADLAPTTAKLIGSQTFPADERQGHALPLST
                     ARSARTPKIVFTFVFDGGGWNVLHQWPDAWPNLKRLMGEGANFRNAIHGSFPAVTACA
                     HATIGTGTYPDEHGITGHNIRDDTGFVRKAYGELGHANPADIVLPTLSDLWYDETQGE
                     AWVGEIGYQIWHLGMMGFGGRVRADGSKPVGVYWDEDGAEDWAPQNPELYRLPTAMPD
                     RGSFDAAYQAFWDAPPDWDEQFTPQGRQAPCCSPPVVAYQGDLIEATLDSEPIGEGGT
                     SLLYTTFKSPDYTGHIYGMGSDWERLQLEAVDAELARVEAMLEERYPGEYVLIVTADH
                     GQCPLPDDVGGVRLDPIQLDRHLAANFNGVTNVVEEITPHEVYLDTRRLWDNGGATVD
                     DVAASLQDYRYRQNIGPYVPVDAIEQDLLDRKEFAAVFGSGYLGTLASADLSGLGETS
                     FTDADPDGIPDAP"
     gene            complement(5521..>6072)
                     /locus_tag="VIX62_01900"
     CDS             complement(5521..>6072)
                     /locus_tag="VIX62_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859023.1"
                     /translation="RHRAVRPGGRSPPSMIQPDRWALEAVTNPSVLRLHVSTPLTDRT
                     IVSCPPEAAPAPLDRLLSIPGVRWLDLHRYRVRLNLGPDGIAGQATVPATETLTDAWG
                     PEAFLDADESPRAFQVAREGDRQVAESLEMAEAHGDAMLMALFAVEGVAEAIAGKDLV
                     LVRLGRMFRWDECSDAVAAALNG"
BASE COUNT          947 a         2127 c         2038 g          960 t
ORIGIN      
        1 atgaaggcgt cggcgatcga gggcgaactc gcgtccggtg gggtggccac gttgatgtag
       61 gccaggaagc tcgcgatcgc ccgcatcttc tggtcgaact cgacgaacac gtcgccgtcg
      121 tgcgcgttcc gctcccgcaa ctcggtggcg gtgcggcccg ggtcgcccca gaaggtcacg
      181 gccgagccat cgggctgggg cgcgaacagc cgcacctcgg gcgcgatcgt ctgcagcccg
      241 tgttgctcca gcttcaggtc cttcacgacg gtgggtcgca gccgcccgat cgtgtgcgtg
      301 atgccgggcg ccgtgaatcc ctcggccacg gtggagttcc gcaggactcc cccgacttcg
      361 ggggcggctt cgagcaccgt gaccttcttg ccgcctttcg cgaggtaggc ggcggccacc
      421 aggccgttgt gccctgcgcc catgacgacc acatcctggc gcgctcccat cagtcgctat
      481 cccttcggtg cttgagcgac atcaggccgc cctccgccca cgcttgcgtt gcttgaggta
      541 ctcgcgagcc gcgatcatgc cgggcgcacc catgatgccc cctccggggt gggtggccga
      601 cccgcacatc cacaggtccc gcaccggcgt ctcgtagcgc gcccacccgg gcaccggacg
      661 gttgaagaac agctgctcga gcgagagttc gccggcgaag atgttgcctt cgctcaggcc
      721 cgtgatgtcc tcgatgtcct tcggggtcac gaactggtgg ccctcgatca gattgcggat
      781 gttggggaag cgctcttcca gcgtgtcgat cacgttctga ccgaaggcct cgcgcttcgt
      841 gtcgtcccac tgctcgccgt tctccaggtg gtagggcgcg tactgcacga agcagctcat
      901 gacgtgcttg ccgggaggcg ccatcgtggg atcgaccagc gtgggcatga tgcagtcgat
      961 gtacgggcgc ttggacggcc ggccgtactt cgcgtcgtcg taggcgcgct ccatgtagtc
     1021 gagcgacggc gagaagctga tcgctccgcg gagccactcc cccacgccgg gcttgcacgc
     1081 gagttcggga agaccggaca gtgccaggtt caccttgccg gaagaaccgc ggtacttgaa
     1141 gcgcatgacc tcgtcgcgga acgtcgggtc caggtcggat tcgtcaagga ggttgatgaa
     1201 cgtctggcgc gagtccagcg aggacatcac cacgtcggcg tcgatctcct cgccgctcga
     1261 gagcaccacg ccggtggcgc gacccccggc cgtcttcacg tggtcaacac ctgcctcggt
     1321 gcggatctcc acgcccagcg agagagcgct gttggcgatc gcgtaggcca cggagccggt
     1381 gccgccccgc ggcacgcccc aggcgcggaa ggcgccgtcg atctcaccca tgtagtggtg
     1441 gagcaggacg tacgccgttc cgggcgatcg tgggccctgg aaggtgccga tgatgccgga
     1501 ggcgctcatc gtggccttca gcggctcggt ctcgaaccac tggtccagga agtcggccgc
     1561 gctcatcgtc atcagctgga tgaacgtggt ctgcagcttc ttcggcaggt ccttgaacgc
     1621 cttggcgagc gggccgagcg gcagccagtg gctcggcgag atcttccccg ggtccggcgg
     1681 cgcgatcgac aggatcggct tgatgaaccg gcccatctcg gccatcagcg agccgtactc
     1741 ctcgtacacc tcggcgtcgg acaggctcca gcggcgcagt tcccggatcg tcttgccgtg
     1801 gtcgttcacg cgccagagat agtcgtcctc gagcggggtg atcgtgccgt ccagcggcag
     1861 gatctccagg ccgtgcttgg ccagctcgag ctcacggatg atctcgggcc gcagcagcga
     1921 taccacatag ctgaataccg agaacttgaa cccgggcacg atctcctcgg tgagggtggc
     1981 gcctcccagg atgtgccggc gttccagcac caggaccttc agcccggcct tcgcgaggta
     2041 ggcggcgttc acgagcccgt tgtgcccgcc tccgatcacg accgcgtcgt acttcttggc
     2101 catggatgcc gctccttctt cccacgggcg cgtccgggcc cggttgcgag gtgacttcag
     2161 gtcaagcggg caccccggaa cacggtgcgg gggcgccggt tgctcatggt agtcgtgcct
     2221 ctgcaccccg gccaacggcc ccttgacttc aggtcaaacg gggcgatgcc atgaggtccg
     2281 gcccctcggc tacccttggg ccctcgaacc gctgtccccg cacgtcccga acaggaggtc
     2341 caccgtgagt ctcggcaccg cgttcttcga gcgcgaacag gcgctgaaca agaagctcgc
     2401 ctggggtgag tggagcggct acttctcggc tccggtgtac gcggacttcc acgacatcga
     2461 atacaacgcg atccgcgagg ccgccggcgt catcgacatc acgccgctct acaagtacga
     2521 ggtctccggc cccgacgccg ttcgcctgct cgatcgcgtg atgacccggg acatgacgaa
     2581 gctgcggatc gaccggatct tctactcgcc gtggtgcgac gaagagggca aggtgctcga
     2641 cgatggcacg atcacccgcc tcgcggagca ccggttccgc atcaccgcgg ccgacccgtg
     2701 ctaccgctgg ttcgtgctga acgccaccgg gttggacgtg gaggtcgccg atgtgagcga
     2761 cgccaccgcg gcgctggccc tgcaggggcg tctgagtcgc gaggtgctgc aggacgccac
     2821 cgggctggac tgggccgacc tgcgctactt cgggcgccgg ggcaccgaga tcgcgggcgt
     2881 gccggtcgac gtcacccgca ccggctacac cggcgaccga ggctacgagc tgtggatggc
     2941 gaaggacgac gccgtcaccg tgtgggaccg catcttcgag gtgggtcagc gctacgggat
     3001 ccaccccgtg ggcatccgtg cgatggacgt cgcgcgcgtc gaggccgggc tgatcctgat
     3061 cgaggcggag tacaccagcg cccgacacgc gatggcgccc gaacagcagt actcaccgtt
     3121 cgagttgaac ttcgacggga tggtcgactt cgccaaggcg agcgatttca acggccggcg
     3181 tgccctgcag gccgagcaaa aggccggagg ccccgagcgc cggctggtgg gcctgcacct
     3241 ggactggtcc ggcatcgagg ggatgtacgc caagcacggg ctggcgcccg cgatcagccc
     3301 ctacgtgcac cgcgacccga tcccggtcta caaggagggc aagcaggtgg gtcgcaccac
     3361 ctcgaccacc tgggggccca cgatcaagaa gatggtctcg ttcggcccgg tcgacaaggg
     3421 gcacagcgtc ctgggctcgc gcctgagcgt cgagtggacc gtggaaggcg agcgcggcaa
     3481 ggtcggcgcc accgtcgtgc cgatgccgtt cttcgacccc gagcgcaagc gcagctagcc
     3541 acgacgccac cggatcgtca caggtcggtc ctcggtcgca tgaccggcgt ttgagaacat
     3601 ggacgggtcc atgcgtccct cccgcgcttc catgctgctc gctgccgtcg gcggcctcgt
     3661 cgggttcctc gtcggggccg ccggttcgtt gatcctgggg ctggcggcgg gcctcatcac
     3721 cttcctgatc tgcctgtggg tgtacgcgcg cgtcactcgc gaggaccgcg caccctccga
     3781 tgacggcgcg gccgaccccg gcagacgacg gttcctggtg ggcgtcgggg tgggcgggtt
     3841 cgcgctcgcg ctggccggcg cgccgatcgg ctggctcgcc cgcaagctgt cccggcccga
     3901 ccccgcgccg atccagacgg cgatggccga gggcctcggt tcggagtaca tggagttcgt
     3961 ccgccgcgcc taccgacccg gacgctcggg cgacatccag atgctgatgg cgccgtacaa
     4021 cagcgcgaac tacgccaacg aatcgctgtc gctggtgccg caggaccctc gcaccagcca
     4081 cgcgagcctg tggatgtacc tcgaacgcat ccccatggtg gtctacgggc ccggcatcgt
     4141 ggcgtcgagc gacagcaccg agcgcgtcac gctcgccgat ctcgccccca ccaccgccaa
     4201 gctgatcggc tcgcagacgt tcccggcgga cgaacgccag ggtcacgcgc tcccgctatc
     4261 gacggctcgc tcggctcgca cgccgaagat cgtgttcacg ttcgtgttcg acggtggtgg
     4321 gtggaacgtc cttcaccaat ggcccgacgc ctggccgaac ctcaagcggt tgatgggcga
     4381 gggcgcgaac ttccgcaacg cgatccacgg ctcgttcccc gcggtgaccg cgtgcgcgca
     4441 cgccacgatc ggcaccggca cctaccccga cgagcacggc atcaccggcc acaacatccg
     4501 cgacgacacc gggttcgtgc gcaaggccta cggcgaactc ggccacgcga accccgccga
     4561 catcgtgctg cccacgctgt ccgacctctg gtacgacgag acgcagggcg aggcgtgggt
     4621 gggcgagatc ggctatcaga tctggcacct gggcatgatg gggttcggcg gacgggtccg
     4681 cgccgacggc tccaagccgg tgggcgtgta ctgggacgaa gacggcgccg aggactgggc
     4741 gccgcagaac cccgagctct atcgtctgcc gaccgcgatg cccgaccgtg gctcgttcga
     4801 cgccgcgtac caggcgttct gggacgctcc tccggactgg gacgagcagt tcacgccgca
     4861 ggggcgccag gcgccctgct gcagcccgcc cgtggtcgcc taccagggcg acctgatcga
     4921 ggcgacgctc gattccgagc cgatcggcga aggtggcacg tcgctcctct acaccacctt
     4981 caagtcgccg gactacaccg gccacatcta cggcatgggc tcagattggg aacggctgca
     5041 gctcgaagcg gtcgacgccg aactcgcccg cgtcgaggcg atgctcgagg agcgctaccc
     5101 cggcgaatac gtcctgatcg tcaccgccga ccacggccag tgcccgctgc ccgacgacgt
     5161 cggcggcgtg cgcctcgacc cgatccagct cgaccggcac ctcgccgcca acttcaacgg
     5221 ggtcaccaac gtcgtggagg agatcacgcc gcacgaggtc tatctggaca cgcggaggct
     5281 gtgggacaac ggcggcgcca ccgtggatga cgtcgcggcg tccttgcagg actaccgcta
     5341 ccgccagaac atcggcccct acgtgcccgt cgatgcgatc gagcaggacc tgctggaccg
     5401 gaaggagttc gccgccgtct tcggcagcgg ctacctggga accctggcga gcgccgacct
     5461 gtccggcctg ggcgagacct cgttcaccga cgccgacccc gacgggatcc cggacgcgcc
     5521 ttagccgttc aacgccgcgg ccacggcgtc actgcactcg tcccatcgga acatccggcc
     5581 cagccgcacc agcaccagat ccttgccggc gatcgcctcg gcgacaccct ccaccgcgaa
     5641 gagcgccatc agcatcgcgt cgccatgcgc ttcggccatc tcgaggctct cggccacctg
     5701 gcgatcgcct tcacgcgcca cctggaaggc tcgagggctt tcgtcggcgt ccaggaacgc
     5761 ctcggggccc cacgcatcgg tcagcgtctc ggtcgccgga acggtcgcct ggcccgcgat
     5821 cccgtcgggt cccaggttca gtcgaacccg gtagcgatgc aggtcgagcc aacgcacccc
     5881 ggggatcgag agcagacgat ccaatggcgc cggcgccgcc tcgggagggc acgacacgat
     5941 cgtccgatcg gtcagcggtg tcgagacgtg gagccgcaac accgatgggt tcgtgaccgc
     6001 ttccagggcc caccggtcgg gctggatcat gctcggaggc gaacgcccgc cgggtcgaac
     6061 agcgcggtgc ct
//
LOCUS       DATDBZ010000079         2772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_433926, whole genome shotgun sequence.
ACCESSION   DATDBZ010000079 DATDBZ010000000
VERSION     DATDBZ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433926"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1075
                     /locus_tag="VIX62_01905"
     CDS             161..1075
                     /locus_tag="VIX62_01905"
                     /inference="COORDINATES: protein motif:HMM:NF042666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6612 family protein"
                     /protein_id="HEY5859024.1"
                     /translation="MKPSRIVRLLSVLAVFALILTACSSGSGTVSDDGPELGQAPGGQ
                     GGGNHDGGDGGDPVALIRAVTAKVEEAGAYRATFEMAITAQGQTIEATGEGEFSDDPL
                     QVHATYRFTGMPGVEGDFEMEMILDGSVMYMRSPMLSQSSGLGTDWISMDLDELVPGF
                     SDLASLSQGQNDPTAQLEYLRGISDAEEVGTEQVAGVETTHYSGTVDLDAAYDRLPDD
                     TRGELKQAIAQARKQFGHGAMPVDVWVDGDGLLRRMTISLESGGNSAVDFGMQMTMEI
                     PEYGIAMHLPIPKDGDVTDVSDLLPAIP"
     gene            complement(1099..1404)
                     /locus_tag="VIX62_01910"
     CDS             complement(1099..1404)
                     /locus_tag="VIX62_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014745962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY5859025.1"
                     /translation="MRFVVTAWDGDDPGAKERRAAVRPVHLENIQPFVDEGHVLVGGA
                     MLDDQGDMIGSTLVVEFDSRSDLDAWITADPYVTGGVWDQVEVHPFRPAVGSWLIEP"
     gene            1488..2741
                     /locus_tag="VIX62_01915"
     CDS             1488..2741
                     /locus_tag="VIX62_01915"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY5859026.1"
                     /translation="MMQRRRLLVAVLAWSMAACSPIASAVLPGASRQPVRIRPADPRP
                     PIERDPVTAPWTVAIDRIVGGQPVSVAVGTGRRLVYQHLGDRARLPASNQKLVLSMAT
                     LDTFGPSHRFITVARPSRPLRQGVLAGDLWLIGSGDPELTPAGLSVLADALHDRGLRT
                     ITGSVVGDVAAFDRRWWAPGWIRGLSRHFVARPTALSLNGNAGLARPELSAATALTDA
                     LRSSGVTVAGSPRTGRAPAHLQPPLAQVRSAPLADILLRQNHGSINFDAEMLTEALGA
                     RVSGSQGSTRGGAAAMESWASDEGVRLRAFDGSGLSHRDRVSTVDLVTLLLDARREPW
                     GHALITSMPSGGEGTLGARLAGVPVHAKTGTLFVTPVSALSGYVRTSDRRLVAFSVLS
                     RGLSKSAAVAIEDAIVRTIAGARLR"
BASE COUNT          392 a          977 c          948 g          455 t
ORIGIN      
        1 cagacggaag ctcctaccag tacgaagcag aggtccggcg caccatgcgc cgggcctcct
       61 tcgtctttgg cttcgtcgta ggcttcgtcg tcggtttcgt atccggatat ccgccgttcg
      121 gcgattcacg tttcgggcgc gcgcggacga taccccttgt atgaaaccct cccggatcgt
      181 tcgcctcctg tccgtcctcg ccgtgttcgc cctcatcctg accgcgtgct cctcgggatc
      241 aggcaccgtc tccgacgacg gccccgaact gggccaagca cccggcggac aaggcggcgg
      301 caaccatgac ggcggcgacg gtggcgatcc tgttgcgctg atccgcgccg tcaccgccaa
      361 ggtcgaggag gcgggcgcct atcgggcgac cttcgagatg gcgatcaccg cgcaaggtca
      421 gacgatcgaa gccaccggcg agggtgagtt ctccgacgac ccgctgcagg tccacgccac
      481 gtaccggttc acgggcatgc cgggggtgga aggcgacttc gagatggaga tgatcctgga
      541 cggttcggtg atgtacatgc ggtcgccgat gctgtcgcag agctcgggcc tgggcaccga
      601 ttggatctcg atggacctgg acgaactggt tccgggattc agcgacctcg cgagcttgag
      661 tcagggtcag aacgacccca cggcgcagct cgagtacctg cgcgggatct ccgatgccga
      721 ggaggtgggt accgagcagg tcgccggcgt cgagacgacg cactactcgg gcaccgtcga
      781 cctcgacgcc gcctacgacc gtctgcctga cgacacgcgc ggtgagctga agcaggcgat
      841 cgcccaggcc cgcaagcagt tcgggcacgg tgcgatgccg gtcgacgtgt gggtcgacgg
      901 cgacgggctc ctgcgccgca tgaccatatc gttggagtcc ggcggcaact cggccgtcga
      961 cttcggcatg cagatgacga tggagatccc cgagtacggc atcgcgatgc atctgccgat
     1021 cccgaaggac ggcgacgtca cggacgtcag cgacctgctc ccggcgatcc cgtaacgtca
     1081 gaccccgggt ttcgaaccct agggctcgat gagccagctc cccaccgccg gccggaacgg
     1141 gtggacctcc acctgatccc acacgcctcc cgtgacatag ggatcggccg tgatccacgc
     1201 gtcgaggtcg gatcgagagt cgaactcgac cacgagcgtc gacccgatca tgtccccctg
     1261 gtcgtcgagc atcgctccgc cgaccaggac gtggccctcg tcgacgaagg gttggatgtt
     1321 ctccaggtgg accggtcgca ccgccgccct ccgctccttc gcaccggggt cgtcgccgtc
     1381 ccaggccgtc accacgaatc gcatgtcgcc tccttcgcgc cggcgcatca gtgggccggt
     1441 cgttccatcg cccgcaacgg ggaccgcggg tcgagcgtct ccacgccatg atgcaacgac
     1501 ggcgactcct cgtggcggtg ctggcgtggt cgatggccgc gtgttcgcct atcgcgtcgg
     1561 ccgtgctccc cggggccagc aggcaacccg tgcgcatccg gcccgccgat ccacggccgc
     1621 cgatcgagcg cgatcccgtt acggcaccct ggaccgtggc catcgaccgc atcgtgggcg
     1681 gccaacccgt gtccgtggcg gtgggaaccg gtcgtcgtct cgtgtatcag cacctgggcg
     1741 accgcgcgcg gttgccggcg tccaaccaga agctcgtgct ctcgatggcg acgctcgaca
     1801 ccttcggtcc ttcgcaccgg ttcatcacgg tggcgcggcc ctcacggcca ctccgccagg
     1861 gcgtgctcgc cggcgacctg tggctgatcg gctccggtga ccccgagctc acgccggccg
     1921 ggctgagcgt gctggccgac gcactgcacg atcgcggact gcgcacgatc accggctcgg
     1981 tggtgggcga cgtcgcggcg ttcgatcgtc gttggtgggc gcccgggtgg atccgaggac
     2041 tctctcgtca cttcgtggcc cgcccgaccg cgctctcgtt gaacgggaac gccggcctcg
     2101 ctcgtcccga gctctcggca gcgaccgcgc tcacggatgc cttgcgatcg agcggggtca
     2161 cggtcgcggg gtcgcctcgc accgggcggg cgcccgccca tctccagccc ccgttggctc
     2221 aggtgcgctc tgcgccgctc gccgacatcc tcctgcggca gaaccacggt tcgatcaact
     2281 tcgacgccga gatgctgacc gaagcgttgg gcgcccgggt cagcgggagc cagggctcga
     2341 ctcggggcgg ggccgcggcg atggagtcgt gggcctccga cgagggcgtc cgtctgcggg
     2401 ccttcgacgg atcgggcctg tcgcaccgcg accgcgtcag cacggtggat ctcgtcaccc
     2461 tgctcctcga cgcccggcgc gaaccgtggg gccatgcgct gatcacgtcc atgccctccg
     2521 ggggcgaggg cacactcggc gcccggctgg cgggcgttcc cgtccatgcg aagacgggca
     2581 ccctgttcgt cacgccggtg tccgcgttgt ccggctacgt gcggacctcc gacaggcggc
     2641 tggtggcgtt ctccgtgctc agcaggggcc tgagcaagtc ggcggcggtg gcgatcgagg
     2701 acgcgatcgt gcggacgatc gcgggtgcgc gcctgcgcta ggctcgcgcc cgtggagttc
     2761 agcgacgtcg tc
//
LOCUS       DATDBZ010000080         2148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1868679, whole genome shotgun sequence.
ACCESSION   DATDBZ010000080 DATDBZ010000000
VERSION     DATDBZ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868679"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VIX62_01920"
     CDS             complement(<1..794)
                     /locus_tag="VIX62_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5859027.1"
                     /translation="MTNATDFDRAFALQRWVLETSSTRTVDLPWGTAFFHDEYPLKYD
                     ANLALLDRPLGTSSVAAVDDVMEDLYSGFRHREIEVRSDEDAGRLAMGLAERGYAVES
                     LVVMAHRRDPDREPAADQVEEVDVATVRDLHMEITRRESWGKEPGIAEIMVAHRDALV
                     ASVDARIFAQRINGTLAGSCELYVHGDVAQIEDVGTLEEFRGKGVARNIVLRAVDEAR
                     AAGASLVFLFADANDWPQHLYGRLGFDVLGPSRLFTRLPEGERTDPS"
     gene            complement(825..1772)
                     /locus_tag="VIX62_01925"
     CDS             complement(825..1772)
                     /locus_tag="VIX62_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY5859028.1"
                     /translation="MDTALIPSRTQIEDTAERIAPHVRRTPVLDVQAGTFGFEAPLVL
                     KLELLQVTGSFKPRGAFNRMLTAQVGESGVVAASGGNFGLAVGHAARELGHRAEIYVP
                     STSPVSKIDRVRATGAEVRVVEGYYDDASHAAAARQEATGAVWMHPYDQPEVVAGQGT
                     IGLELSQQVPDADTVVVSVGGGGLIGGIAAWFASEVAVVAVEPVTSTCLGSALEAGEP
                     VPVEVAGIAADSLGTRMVGDIAFAVAREHVGTAVTVTDDAIREAQRAIWREFHLIAEP
                     GGATALAALMAGAYRPRSGERIVVLVCGANGDPAPVLGV"
BASE COUNT          334 a          803 c          707 g          304 t
ORIGIN      
        1 gacgggtccg tgcgctcccc ttccggcagg cgggtgaaca gccgtgacgg gccgagcaca
       61 tcgaacccca accgcccgta gagatgctgc ggccagtcgt tcgcgtcggc gaacaggaac
      121 accagcgagg cgcccgccgc ccgcgcttcg tccaccgcgc gcagcacgat gttgcgtgcc
      181 acccccttgc cacggaactc ctcgagcgtg ccgacgtctt cgatctgcgc gacgtcaccg
      241 tgcacgtaga gctcgcacga acccgccagc gttccgttga tgcgctgggc gaagatccgc
      301 gcatcgacgc tggcgacgag ggcgtcgcgg tgcgccacca tgatctcggc gatcccgggc
      361 tccttgcccc acgattcgcg ccgggtgatc tccatgtgca gatcccggac cgtggccacg
      421 tcgacctcct ctacctggtc cgcggctggc tcgcgatcgg gatcgcgacg gtgggccatg
      481 accaccaggg actccaccgc gtagcctcgt tcggccagac ccatcgcgag gcggccggcg
      541 tcctcatccg agcgcacctc gatctcacgg tgacggaacc ccgaatacag gtcctccatc
      601 acgtcgtcga ccgctgccac cgacgaggtc cccagcggcc ggtccagcaa ggcgaggttg
      661 gcgtcgtatt tcagcgggta ctcgtcgtgg aagaacgccg tgccccacgg gaggtcgacc
      721 gtccgggtgg aactggtctc cagcacccag cgctgcaacg cgaaggcgcg gtcgaagtcg
      781 gtggcgttcg tcacggccgg tgagcctact agcgggtggc gcgctcagac gccgagcacg
      841 ggcgcagggt cgccgttggc gccgcacacc aggacgacga tccgttcgcc gctccgaggg
      901 cgataggcac ccgccatgag cgccgccagg gcggtcgccc ctccgggctc ggcgatcagg
      961 tggaactcgc gccagatcgc gcgctgcgcc tcacggatcg cgtcgtcggt gaccgtgacc
     1021 gccgtcccga cgtgctcacg agccacggcg aaggcgatgt cgccgaccat gcgggtgccc
     1081 agcgagtccg ccgcgatgcc ggcgacctcc accgggacgg gttcgccggc ttccagggcg
     1141 ctcccgagac acgtggaggt cacgggctcg acggccacca ccgcgacctc gctcgcgaac
     1201 caggccgcga tcccgccgat cagtccgccg ccgcccacgc tcaccaccac cgtgtcggcg
     1261 tcgggtacct gctgggagag ctcgaggccg atcgtgccct ggccggccac cacctcgggc
     1321 tgatcgtagg gatgcatcca cacggcaccg gtcgcttcct gtcgcgcggc ggcggcatgg
     1381 gacgcatcgt cgtagtagcc ctcgaccacg cgcacctcgg cacccgtggc tcgcacacgg
     1441 tcgatcttcg agaccggcga cgtggacggc acgtagatct ccgcgcggtg gcccagttcg
     1501 cgcgcggcgt gacccaccgc gaggccgaag ttccccccgc tcgccgccac cacgccgctc
     1561 tcccccacct gggccgtgag catgcggttg aacgccccgc gcggcttgaa cgacccggtc
     1621 acctgcaaca gctcgagctt caggacgagc ggggcctcga aaccgaaggt gcccgcctgc
     1681 acgtcgagca cgggcgtccg acgcacgtgg ggcgcgatgc gttccgcagt gtcctcgatc
     1741 tgggtgcggg acgggatcaa cgcggtatcc atcgcgccga tgctgtcaca gcggcagcgt
     1801 ccacgacctg cccaccagac ccagagacgc caggatcgcc tgcgaagcgt cgcgggaccg
     1861 cggctcccag tcctcgcgca aacggtcgtg ataccacgcc cgagccagcg cgcacgtggt
     1921 ggcaaggtcc agcgtcttcc ccggctcgac gccttcccgc tcgcaccagg tccggacatg
     1981 tccctccgac cggaaggctc ggatggtgcg tcaggtgaag acgatgtcgt cccaccacct
     2041 ggatgccggc accaggaagt ggacgagggc gtcggcggca cgtgcctcac tcgcacggat
     2101 ctccagccgc atcacctcgc cgcagtcggg acaatcggtg tcgatgac
//
LOCUS       DATDBZ010000081         7646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_232929, whole genome shotgun sequence.
ACCESSION   DATDBZ010000081 DATDBZ010000000
VERSION     DATDBZ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232929"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..788
                     /locus_tag="VIX62_01930"
     CDS             222..788
                     /locus_tag="VIX62_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5859029.1"
                     /translation="MRTTREPDNDAALMRAFASRDRNAADTLYARFASRIYGLGIVML
                     GSDAAAQDLVQDTFVKLWRNADRYDTTRGKLETWVLLVARSTAIDSLRRRVLEVRSLE
                     ATGRPREADPGAGPEEIAETGDMVARARRAMEQLSGEQRAALELAYFGGKTSTEVAEL
                     EGIPVGTAKTRIRTALLRLRESMAEGAP"
     gene            785..1504
                     /locus_tag="VIX62_01935"
     CDS             785..1504
                     /locus_tag="VIX62_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY5859030.1"
                     /translation="MTTCERVRERLPEHVLGTLNEQDDAEVRRHLRGCAGCRAEMSAL
                     GEGMASFARAAHDQPPPEELEDRVLAVLDEEWRAEAEAEPSRRRTLSWLAVAAVVAIV
                     VLSVGFGVFHASRAASVADSANSYEQLLHILGGEEFRAGELHAAGSHPVEGSIVIYDS
                     HVDQSWVVVFVRTSGLTGEAVATLHAADGSTIDTWPVEIDRDGDGAGWLVTSVDLASF
                     DRVTLTGPDGQPLAAGEIGEL"
     gene            complement(1514..2506)
                     /locus_tag="VIX62_01940"
     CDS             complement(1514..2506)
                     /locus_tag="VIX62_01940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5859031.1"
                     /translation="MRITFSEDGRDFHRRGWAELVDADPAGTVFHTPAYLKLYWEEFG
                     ETPDHLLLAFAEEDDGAQVGAVAFERIGQTLRFLGGTEVTDYMGPVGVPERQDAIAKE
                     LWTALLTREDWADADLRGLPEDQPWLGLLRDAAAGLGLTVEETDDQNGVAPFLPLPGT
                     WEAYLEALPSKLRHEIRRKARKLTEEAGEYTIVPATDETLEPLLDRFVELHRLSEGPK
                     GVFMVPGMEIFFRRLGEAFCAEGIFRLTFIEVAGQLAAGTIGFEFGGTSFLYNSAFDR
                     TWGNLAPGMVLVGEDIKLAIEHGCTAFDLLKGDYQYKYRFGASPRAIKRLIVTR"
     gene            complement(2547..4700)
                     /locus_tag="VIX62_01945"
     CDS             complement(2547..4700)
                     /locus_tag="VIX62_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY5859032.1"
                     /translation="MSRRAWRWRVGSTVVCVWVLTMTLVIGVGTAEAASACGTSSSLS
                     LCLSVPDGVLSGDVPVTITFAGSSADVAELHVSWGTSSTTSTSLHNEYEAPWTFTWPT
                     DRYRDASQYLNVRVRRKGASAFGSPVWLTTTIENGNDTSVPPTPADWATNFVPRPATG
                     DPVIAAVGDGGNGTSAGDAVVASITSSPASMLLYLGDIYEEGTPTEWENNYGHASFDP
                     SGPATRWGTMASWTRPTMGNHEGGNIPAYRDYWHQVPDFDTFTFGGVRYLQLNSECAR
                     IPGGCGAGSPQYVFVQQTLASSAQACIVAFWHRPVLSLNDDNQPMRPIWKLLADGGGD
                     LVLNGHAHTMEAYRPMNGALETGKSDSHMVELISGNGGHFGATGSDSDTRAAWIRNSV
                     PGAAYVTAVGGGTGQPTSLAWAFRDTAGAQVSANGVPGAGSVQCGADLQAPDAPGKPT
                     GVSSAPGTIDLTWAASHDDQASTLTYRIYRDGAVTPTATISSSSTGTVTWRDSGLTGG
                     TQHSYAVEAFDGVNTGPRSPTSELITVMAGASEILSDRFDAGLGGWTVAMNVAIDSSQ
                     GGAAPPSARISVNGVKGFMWRDFGAGYPLVCVSMRINLQSQTSTPVTLIKLRTSSDAP
                     IGGLQVTRSRVLQVVSDVSGSTFPSTTLPTGWHTVKLCGSTGGSGTWQLSVDGGAVSS
                     WTTNNGTTPFARVQFGDKGTKTITLNLDDVVVTQS"
     gene            complement(4876..6669)
                     /locus_tag="VIX62_01950"
     CDS             complement(4876..6669)
                     /locus_tag="VIX62_01950"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY5859033.1"
                     /translation="MRRMIAALIVAIGALLATSGIARAQFTEQIDGYDVAIELRTDGS
                     LRITEVIDYDFGVTEHHGIFRDVPTRVHYDDTYDRVYPLTVESVSATGGASADYTTER
                     DGSLTRIKIGDPEETVSGDHRYTIVYTVEGATNGFTDHDELYWNAIGDEWPVSIDDVT
                     VQVEAPGDITDSLCFQGPERSTLPCDGQKVRGASATFRQQHLDPYSGVTIVLAFPKGV
                     LDAPAPILEERWSVSSAFRLTPATGGVSGALLLLIVGGAAVTLWRTGRDRRYQGSPID
                     QVMGNPTGDDEAVPIFDADTEAPVEFAPPGDMRPGQMGTLVDEQANTLDVSATIVDLA
                     VRGFLMIQEIPKEGWFGKPDWTLIKLEESEAGLLTYERRLLNALFRDGTEVTISELRN
                     TFAARLEGVEESLYVDVVNRGWFRSRPDKVRSTWQARGVVVLILGAALTFVLARWTHW
                     GLLGIPLILGGLLLVMFAKRMPARTASGTAMLRRVRGFRRVIETAETHMSQWAEKEMI
                     FTTFLPYAVVFGCTDRWAKAFAALGVQPDTSWYVSPRPFVYTEFAQSMDGFAVTTGGT
                     IASTPSGSGSSGFGGGGFSGGGGGGGGGGSW"
     gene            complement(6666..>7646)
                     /gene="cax"
                     /locus_tag="VIX62_01955"
     CDS             complement(6666..>7646)
                     /gene="cax"
                     /locus_tag="VIX62_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEY5859034.1"
                     /translation="LAIFLTAALAILPLAGLIGRSTEQLALHTGPRVGGLINATFGNV
                     TELIIAIFLLMDDKVEIVKASLTGSILGNLLLVMGLSLFLGGVKHSVQSFNARAASVH
                     ATSLALAVTGLLMPALFALGGRESFLQREVVSGTVAAVLIALYLAALAFTLITHQHLF
                     RAPEEGEVAAWTKNRAIASLLGATALVALMSEFLVGSLEPALAKLGLSELFVGLIVIP
                     IIGNAAEHSSAVMFALRNKLDVTLEIAIGSSTQIALFVAPALVFISLLIGHPMDFVFS
                     TFEVAAVAISTLLIALISLDGESNWLEGAQLCGAYVIMAISFFFVEHLHP"
BASE COUNT         1246 a         2703 c         2537 g         1160 t
ORIGIN      
        1 cacgatcagg ggacgcgccc cccaactggc gctcgttgcg ggccaacgcc tcaagcccga
       61 cgacgccctg ctgagcggcg ccgtggtgct cgggggcgat cgcgaggtcg ccgacctggt
      121 gctgcgcaac ctgcgcgcct acccgtagcg ctcgatccgg cccgccggga acgaaccatc
      181 gccctggctc gtccgtactc cctgcgagta ccgtgcgaac cgtgagaacc acccgcgagc
      241 cggacaatga cgccgccctg atgcgtgcgt tcgcttcgcg cgaccggaac gcggccgaca
      301 ccctctacgc gcggttcgcc tccaggatct acggattggg gatcgtgatg ctcggcagcg
      361 acgcggcggc ccaggacctg gtccaggaca ccttcgtcaa gctctggcga aacgccgatc
      421 ggtacgacac cacccgcggc aagctcgaga cctgggtgct gctggtcgcc aggtccacgg
      481 ccatcgactc gctgcggcgg cgggtgctgg aggtgcgatc gctcgaggcc accggacggc
      541 ctcgtgaggc cgaccccggc gccggccccg aggagatcgc cgagaccggc gacatggtgg
      601 cgagggcccg ccgcgccatg gagcagctct cgggtgagca gcgcgccgcg ttggaactgg
      661 cctatttcgg cggcaagacc agcaccgagg tcgccgagct ggaagggatc cccgtcggca
      721 cggcgaagac ccggatccgc acggccctgc tcaggcttcg ggagtcgatg gcggagggtg
      781 ccccatgacg acgtgtgagc gcgtccgtga gcgcctcccc gagcacgtcc tcggcaccct
      841 gaacgaacag gacgacgcgg aggtccgccg ccacctgcgc ggatgcgcag gatgccgggc
      901 cgagatgtcg gcgctgggcg aggggatggc gtcgttcgcg cgagccgcgc acgaccagcc
      961 gcctcccgag gaactcgagg accgtgtcct ggccgtcctc gacgaagagt ggcgtgctga
     1021 ggcggaggcg gaaccgagcc gccggcggac cctgtcctgg ctggccgtcg cggccgtggt
     1081 ggcgatcgtg gtgctctcgg tggggttcgg cgtgttccac gcgagcaggg cagcgtcggt
     1141 cgccgacagc gcgaacagct acgagcaact cctgcacatc ctcggcggcg aagagttccg
     1201 cgccggcgag ctccacgcgg cgggttccca cccggtggag ggcagcatcg tgatctacga
     1261 ctcccacgtc gatcaatcgt gggtcgtggt gttcgtccgc acatcgggac tcaccggcga
     1321 ggcagtcgcg acgctgcatg ccgccgacgg atccacgatc gacacctggc ccgtggagat
     1381 cgatcgcgac ggcgacggcg ccggctggct cgtcacgtcg gtcgacctcg cgtcgttcga
     1441 tcgcgtcacg ctcaccggtc ccgacggcca accccttgct gccggcgaga tcggtgagct
     1501 gtgacgtccg ccgtcagcgc gtgacgatca gccgtttgat cgcgcgcggg gaagccccga
     1561 atcggtactt gtactggtag tcgcccttca acagatcgaa cgcggtacat ccatgctcga
     1621 tcgcgagttt gatgtcttcg cccaccagca ccatgcccgg agccaggttc ccccaggtgc
     1681 gatcgaacgc cgagttgtac aggaacgacg tgcccccgaa ctcgaatccg atcgtacccg
     1741 ccgccagctg cccagccacc tcgatgaacg tcagccggaa gatcccttcg gcgcagaacg
     1801 cctccccgag ccgacggaag aagatctcca tccccggaac catgaagacg cctttgggtc
     1861 cctcggacaa gcggtggagc tccacgaagc gatcgagcaa cggctccaag gtctcatcgg
     1921 tggccggcac gatcgtgtac tcgcccgctt cctcggtgag cttgcgggcc ttccgcctga
     1981 tctcgtgccg cagcttcgac ggcagcgctt ccaggtaggc ctcccacgtt cccggcaacg
     2041 gcaggaacgg ggcgacaccg ttctggtcgt cggtctcctc gacggtgagc cccaggccgg
     2101 cggcggcgtc ccgcaggagc cccagccacg gctggtcctc ggggaggcca cgcagatcag
     2161 cgtcggccca gtcctcccgg gtgagcagcg cggtccagag ctccttcgcg atcgcgtcct
     2221 gccgttcggg cacgcccacc ggtcccatgt aatcggtgac ctcggtgcca cccaggaacc
     2281 gcagggtctg cccgatccgt tcgaaggcga cggctcccac ctgggcgccg tcgtcctctt
     2341 ccgcgaacgc cagcaggaga tgatctggcg tttcgccgaa ctcctcccag tagagcttca
     2401 ggtacgccgg cgtgtggaac accgtgccgg cagggtcggc gtcgacgagc tcggcccatc
     2461 cgcgccggtg gaaatcacgc ccgtcctcgg agaaggtgat ccgcatccgc gcccatgcta
     2521 cgggccgaga gcgctcggcg ggcgtttcag ctctgggtga ccacgacgtc gtcgaggttc
     2581 agggtgatgg tcttcgtgcc cttgtcgccg aactggacgc gggcgaacgg cgtcgtcccg
     2641 ttgttcgtgg tccacgatga caccgcaccg ccgtccaccg acagctgcca ggtccccgat
     2701 ccgcccgtgc tcccgcacag cttcacggtg tgccatcccg tcggcagggt cgtcgacggg
     2761 aacgtcgacc cggacacgtc ggagacgacc tggagcacgc ggctccgggt gacctggagg
     2821 ccaccgatcg gggcgtcgga cgacgtgcgg agcttgatca gcgtgaccgg ggtgctggtc
     2881 tgcgactgga ggttgatcct catgctcacg cataccaggg ggtacccagc gccgaagtca
     2941 cgccacatga atcccttcac gccgttcacg gagatccggg cgctcggtgg cgccgctccc
     3001 ccttgcgacg agtcgatcgc gacgttcatc gccacggtcc atccgccgag gccggcgtcg
     3061 aaccggtcgc tgaggatctc gctcgcgccg gccatcaccg tgatgagctc ggacgtcgga
     3121 ctccgaggcc cggtgttgac cccgtcgaac gcctccacgg cgtaggagtg ctgggtcccc
     3181 ccggtgagtc cggaatcgcg ccaggtcacg gtccccgtcg aggagctcga gatcgtcgcc
     3241 gtgggcgtga ccgcgccgtc acggtagatc cggtaggtca gggtgctcgc ctgatcgtca
     3301 tgagaagcgg cccatgtcag gtcgatcgtg ccgggtgcgg agctgacccc tgtcggcttc
     3361 ccgggcgcat ccggcgcctg caggtcggcc ccgcactgga cggagccggc gccgggcacg
     3421 ccgttggccg acacctgcgc gcccgcggtg tcgcggaacg cccacgccag cgacgtgggc
     3481 tgacccgtgc cgccgcccac cgccgtgacg taggcggcgc cggggacact gttgcggatc
     3541 cacgccgctc gcgtgtcgct gtccgatccc gtcgccccga agtggccgcc gttgccggag
     3601 atcagctcca ccatgtggga gtcggacttc ccggtctcga gcgcgccgtt catcggtcga
     3661 taggcctcca tcgtgtgcgc gtggccgttg aggacgaggt cgccgccgcc gtcggccagc
     3721 agcttccaga tcggtcgcat cggctggttg tcgtcgttca ggctcagcac gggccggtgc
     3781 cagaacgcca cgatgcacgc ctgggcggag ctcgccagcg tctgctgcac gaagacgtac
     3841 tgcggactcc ccgcgccgca tccaccgggg atgcgagcgc actcgctgtt gagttgcagg
     3901 taccgcaccc cgccgaacgt gaaggtgtcg aagtcgggca cctggtgcca gtagtcccgg
     3961 taggcgggga tgttcccgcc ctcgtggttg cccatcgtgg gcctggtcca ggacgccatc
     4021 gtgccccacc tcgtagccgg gccgctgggg tcgaacgagg cgtgcccgta gttgttctcc
     4081 cactcggtgg gcgtgccttc ctcgtagatg tcacccaggt acaggagcat cgaggccgga
     4141 gacgaggtga tggacgccac gaccgcatcg cccgccgacg tgccgttccc tccgtccccc
     4201 acggcggcga tcacgggatc gccggtggcc ggccggggca cgaagttcgt ggcccagtcg
     4261 gccggcgtgg gcggcaccga ggtgtcgttg ccgttctcga tcgtggtcgt gagccacacg
     4321 ggcgaaccga acgcggaggc gccctttcgc cggacccgga cgttcaggta ctggctcgcg
     4381 tcccggtagc gatccgtcgg ccaggtgaac gtccacggcg cctcgtactc gttgtgcagg
     4441 gacgtcgacg tcgtggagct ggtcccccag ctcacgtgca gctcggccac gtccgccgac
     4501 gagccggcga aggtgatcgt caccgggacg tcgcccgaca ggaccccgtc ggggacggac
     4561 agacagagcg acagactcga tgacgtgccg cacgccgatg cggcctcggc cgtgccgaca
     4621 ccgatcacca acgtcatcgt gaggacccac acgcagacca cggtggatcc cacccgccaa
     4681 cgccacgccc tcctgctcat cgttccaacc tccggcctgc ccttggccat ggtccggggg
     4741 gatcgacggg atcccacgag ggtggaactc ccagccgccg aacgatcgct gccagatcgc
     4801 ctgtcgtttg tcggcttgga cggtagcgcc ggttccgcca tccggcaact cccgtgggat
     4861 gacggtgctg tcacctcacc aagaaccgcc accaccgccg ccgccgccac cgccggagaa
     4921 cccgccgccg ccgaacccgg acgatcccga gcccgagggc gtcgaggcga tcgtgccccc
     4981 ggtggtcacc gcgaacccat ccatcgactg cgcgaactcg gtgtacacga acggacgcgg
     5041 ggacacgtac cagctggtgt ccggctgcac ccccagggcc gcgaacgcct tcgcccacct
     5101 atccgtacac ccgaagacca cggcgtacgg caggaaggtg gtgaagatca tctccttctc
     5161 cgcccactgc gacatgtggg tctccgcggt ctcgatcacg cggcggaacc cccggacccg
     5221 ccggagcatg gcggtgccgc tcgcggtcct cgccggcatc cgcttcgcga acatcaccag
     5281 gagcagcccg ccgaggatca acgggatgcc gagcagcccc cagtgcgtcc agcgcgccag
     5341 gacgaacgtc agcgcggccc ccaggatcag caccaccacc ccgcgcgcct gccacgtgga
     5401 acggaccttg tcgggacgcg accggaacca gccccggttc acgacgtcga cgtacagcga
     5461 ttcctcgacg ccctccaacc gagccgcgaa cgtgttccgc agctccgaga tcgtgacctc
     5521 ggtgccgtcg cggaacagcg cgttcaacag gcggcgctcg tacgtgagca gccccgcctc
     5581 cgattcctcg agcttgatca gcgtccagtc gggcttgccg aaccacccct ccttcgggat
     5641 ctcctggatc atcaggaacc cgcgcaccgc gaggtccacg atggtcgccg agacgtcgag
     5701 ggtgttggcc tgctcatcca ccaaggtgcc catctgcccg ggccgcatgt cgcccggggg
     5761 cgcgaactcc accggcgcct cggtgtccgc atcgaagatc ggcacggcct cgtcgtcgcc
     5821 ggtgggattg cccatcacct gatcgatggg cgaaccctga taccggcggt cacgcccggt
     5881 gcgccatagc gtcaccgcgg cgccacccac gatcagcagg aggagcgcac ccgagacccc
     5941 gccggtcgcc ggcgtaaggc ggaaggcgct ggagaccgac cagcgttcct ccaggatcgg
     6001 cgccggtgca tccagcaccc ctttcgggaa ggccaggacg atcgtgacgc cgctgtaggg
     6061 atcgagatgc tgctggcgga acgtcgcgct ggcgccccgg accttctgcc cgtcgcacgg
     6121 caacgtggaa cgctccggac cctggaagca cagggagtcg gtgatgtcac cgggcgcctc
     6181 gacctggacc gtgacgtcgt cgatcgagac cggccactcg tcgccgatcg cgttccagta
     6241 cagctcgtcg tggtcagtga acccgttcgt ggcgccctcg acggtgtaca cgatcgtgta
     6301 ccggtgatcg cccgagacgg tctcctcggg atcaccaatc ttgatccgcg tcaaggatcc
     6361 gtcgcgctcg gtggtgtagt cggccgaggc gcctcccgtc gcgctcaccg actccacggt
     6421 gagcggatac acccggtcgt acgtgtcgtc gtagtgcacc cgggtgggga cgtcacggaa
     6481 gatcccgtgg tgttcggtga ccccgaagtc gtagtcgatg acctcggtga tgcgcaggga
     6541 tccgtcggtc cgcagctcga tcgcgacgtc gtaaccgtcg atctgctcgg tgaactgggc
     6601 gcgggcgatg cccgaggtgg cgagcagcgc cccgatggcg acgatgaggg ccgcgatcat
     6661 ccgtctcatg gatgcagatg ttccacgaag aagaacgaga tggccatgat cacgtaggcg
     6721 ccgcacagct gagctccctc gagccaattg ctctccccat ccagtgagat caacgcgatc
     6781 agcaacgtcg atatggccac cgcggccacc tcgaaggtac tgaacacgaa gtccatcggg
     6841 tgcccgatca gaagcgagat gaacaccagg gccggagcca cgaacaaggc gatctgcgtg
     6901 ctcgacccga tcgcgatctc cagcgtcacg tccagtttgt tccgcagcgc gaacatcacc
     6961 gccgacgaat gctccgcggc gttgccgatg atcgggatca cgatcaggcc gacgaagagc
     7021 tcggacaacc ccagcttggc gagcgccggt tcgagcgagc ccaccaggaa ctccgacatc
     7081 aacgccacca gcgcggtggc acccagcagc gaggcgatcg cccggttctt cgtccacgcc
     7141 gccacctccc cttcctcggg ggcgcggaag aggtgctgat gcgtgatcag ggtgaacgcc
     7201 agcgcggcga ggtagagcgc gatgagcacg gctgccaccg tgccgctcac cacctcacgc
     7261 tgcaggaacg attcgcgacc cccaagggcg aacaacgccg gcatcaacag gccggtcacg
     7321 gccagagcca acgaggtggc gtgcaccgag gccgcgcgcg cgttgaagga ctgcaccgaa
     7381 tgcttgaccc cacccaggaa cagcgacagt cccatgacca gcaagaggtt gccgaggatc
     7441 gatccggtca gggacgcctt cacgatctcg accttgtcgt ccatcagcag gaagatcgcg
     7501 atgatcagct cggtcacgtt gccgaacgtg gcgttgatca gcccgccgac cctcggcccg
     7561 gtatggagtg cgagctgctc ggtggaacgg ccgatcagac ccgcgagcgg caggatggcg
     7621 agcgctgcgg tcaggaagat cgcgag
//
LOCUS       DATDBZ010000082         2754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2893515, whole genome shotgun sequence.
ACCESSION   DATDBZ010000082 DATDBZ010000000
VERSION     DATDBZ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893515"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..564)
                     /locus_tag="VIX62_01960"
     CDS             complement(13..564)
                     /locus_tag="VIX62_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5859035.1"
                     /translation="MTRLFLVRHGRAAAGWDDDADPGLDDIGRRQATRVAARLAPAGP
                     LDIWTSPLRRCRETASPLAATWGTAPIVQNAVAEIPSPEGIEMGARVPWLRAAMAGRW
                     GELGERYTSFRDGAAAQMLTATRDTVVFSLFVAINAVIGAALGDDRLVIRSLDNCSVT
                     IIDITDGRLVLVEGGEEADTLIR"
     gene            complement(561..1370)
                     /locus_tag="VIX62_01965"
     CDS             complement(561..1370)
                     /locus_tag="VIX62_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018160081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY5859036.1"
                     /translation="MSTLVSFHAHPDDEAIATGGSLARASAEGHRVVLVIATHGEWGE
                     VPDDLAPGETLVHRREAETAESARILGIHRVAWLGYEDSGMTGWEQNANVGSFHQAPV
                     DEAAERLAAILREERADVLTVYDWHGNYGHPDHIKVHHVGHRAADLAGTERVAEATMN
                     RTAVRRLVEMARDHGMQLGRDESDFDIDGPADDGNPMGMEEHEITLAVDVSPFVWLKR
                     DAVKAHRSQVTDTSFFAEMPDEMFAASFGTEWFIERGVEQEGGPRRGWFFE"
     gene            complement(1377..2528)
                     /locus_tag="VIX62_01970"
     CDS             complement(1377..2528)
                     /locus_tag="VIX62_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY5859037.1"
                     /translation="MAGPLAGYRIIEIAGIGPGPFAAMMLADMGAEVIRVERVQAVRS
                     PAPDRPYLDALSRGRRSIAIDLKQPEGVETLLGLVERADALIEGFRPGVMERLGIGPE
                     ECAARNPKLVFGRMTGWGQSGPYASSAGHDINYISLAGALAHFGRHGGPPTPPLNMVG
                     DFGGGGMFLAFGVVCALLEAQKSGTGQVVDTAMVDGSAVLMTMFWAFKEMGVFDEHAR
                     GTNLLDTGAHFYDVFRCKDGGYISIGSIESQFYAELLRLTGMADDPEFARQMDKDLWP
                     HLKSRLIRVFEEKTRDEWCAIMEHTDVCFAPVLTMSEAAAHPHNVDRGTFIEVGGVVQ
                     PAPAPRFSRTTAEVSRPPAHAGQHTREVLADWGVAPDAIDQLFASGAVK"
BASE COUNT          431 a          959 c          917 g          447 t
ORIGIN      
        1 ctcgatcggc gatcacctga tgagggtgtc ggcctcctcg ccgccctcca cgagcacgag
       61 ccgcccgtcg gtgatgtcga tgatcgtgac cgagcagttg tccaggctcc ggatcacgag
      121 ccggtcgtcg ccgagcgccg cgccgatcac ggcattgatg gcgacgaaga ggctgaacac
      181 gacggtgtcg cgggtagcgg tcagcatttg tgctgccgct ccgtcacgga atgacgtgta
      241 ccgctcgccg agctcacccc atcttccggc catcgccgct cggagccatg gcacgcgggc
      301 gcccatctcg atgccctccg gtgacgggat ctcggcaacc gcgttctgga cgatgggcgc
      361 ggttccccac gtcgccgcga gcgggctcgc cgtctcgcgg caccgccgca gcgggctggt
      421 ccagatgtcg agcgggcccg ccggcgcgag ccgggctgcg acacgcgtcg cctggcggcg
      481 gccgatgtca tcgagccctg ggtcagcgtc gtcgtcccat ccggccgctg cccgcccatg
      541 gcggacgagg aacaggcgcg tcactcgaag aaccagcccc gccgcggccc accctcctgc
      601 tcgacgccgc gctcgatgaa ccactcggtg ccgaacgatg cggcgaacat ctcgtcgggc
      661 atctcggcga agaacgacgt gtcggtgacc tggctgcggt gtgccttgac ggcatcacgc
      721 ttcaaccaga cgaacgggct cacgtcgacc gcaagggtga tctcgtgttc ttccatgccc
      781 atcgggttgc cgtcgtcggc agggccgtcg atgtcgaagt cgctctcgtc ccgacccagc
      841 tgcatcccat ggtcgcgcgc catctcgacc aagcggcgga ccgcggtccg gttcatggtc
      901 gcctcggcga cacgctcggt accggccaga tcggccgctc ggtggccgac gtgatgcacc
      961 ttgatgtggt cgggatggcc gtagttgccg tgccagtcgt acaccgtcaa cacgtccgcc
     1021 cgctcctcac gcaggatcgc ggcgaggcgc tcagccgctt cgtcgacagg cgcctggtgg
     1081 aacgaaccga cgttcgcgtt ctgctcccat ccggtcatac ccgagtcctc gtagccgagc
     1141 caggccacgc ggtgaatacc gaggattcgc gccgattcgg cggtctccgc ctcccggcgg
     1201 tgaacgagcg tctcaccggg cgcgagatca tcaggcacct caccccactc gccgtgggtc
     1261 gcgatcacca gcacgacgcg atggccttcg gcggaagccc gtgccagcga gccgccggtg
     1321 gcgatcgctt cgtcgtcggg gtgggcgtgg aaggagacga gggtgctcat gtcacgtcac
     1381 ttcacggcgc cggacgcgaa cagctggtcg atcgcgtcgg gcgcgacgcc ccagtcggca
     1441 agtacctcgc gcgtgtgctg accggcgtgg gcgggagggc ggctgacctc cgccgtggtg
     1501 cgcgagaagc ggggcgcggg ggccggctgg acgacaccac cgacttcgat gaacgtgccc
     1561 cgatcgacgt tgtgcgggtg cgcagcagct tcactcatcg tgagcaccgg ggcgaagcac
     1621 acgtcggtgt gctccatgat ggcgcaccac tcgtcgcgcg tcttttcctc gaacacgcgg
     1681 atgaggcggc tcttgaggtg cggccagagg tccttgtcca tctgccgtgc gaactccgga
     1741 tcatccgcca tgccggtgag gcggagcagc tcggcataga actgcgattc gatcgaaccg
     1801 atcgagatgt acccgccgtc cttgcaccgg aacacgtcgt agaagtgcgc gcccgtgtcg
     1861 agcaggttgg tcccgcgagc atgttcgtcg aacacgccca tctccttgaa cgcccagaac
     1921 atggtcatga gcacagcaga gccgtcgacc atggcggtat cgaccacttg gccggtgccg
     1981 ctcttctgcg cttcgagcag cgcacacacg acaccgaagg cgaggaacat gccaccaccg
     2041 ccgaagtcac cgaccatgtt gagcgggggc gttggcgggc cgccatgacg accgaagtga
     2101 gcgagcgcgc ccgcgagcga gatgtagttg atgtcgtgcc ccgcgctgct cgcgtacggc
     2161 cctgattgac cccacccggt catacggccg aagacgagct tgggattgcg ggcggcgcac
     2221 tcctccggtc cgatgccgag ccgttccatg acaccgggcc gaaagccctc gatgagcgca
     2281 tcggcgcgtt cgacgagacc gaggagcgtc tccacgccct cgggctgctt caagtcgatg
     2341 gcgatgctcc gccggccgcg cgacagcgca tcgagatacg gacggtccgg tgctgggctg
     2401 cgcaccgcct gcacccgctc gacccggatc acctcggccc ccatgtcggc cagcatcatc
     2461 gccgcgaacg gccccgggcc gatgccggcg atttcgatga tccggtagcc cgcgagcggt
     2521 cctgccatgg tgatctccct ttgacctgga tggtgaatcg acaacgcgcc cttcggccgg
     2581 atcgttgtgg ccgcgaccgc acggagtttc gttccggacg gcgcgttcgg tcaacgtggc
     2641 gccaccaacg tcacaccggt cggatgccag cacgcgacgg agctgtccac cggcgatggc
     2701 tcgagctgcg gatcgtcgga cgagcaccgt ggtccggctc ggccacatct cggc
//
LOCUS       DATDBZ010000083         2993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2074599, whole genome shotgun sequence.
ACCESSION   DATDBZ010000083 DATDBZ010000000
VERSION     DATDBZ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074599"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..568
                     /locus_tag="VIX62_01975"
     CDS             116..568
                     /locus_tag="VIX62_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY5859038.1"
                     /translation="MTTTAAYADIPGLAGTDLGFTDWLEITQESVNTFADATGDHQWI
                     HVDPERAKDGPFGGPIAHGFLTLSLAVKFWTDLLDVDGVGTKVNYGLDKVRFISPVKV
                     GSRVRMNAVIAEVTEVAGGFQLAVDQTIEIEGGTKPAVVARGLYRFYA"
     gene            669..2183
                     /locus_tag="VIX62_01980"
     CDS             669..2183
                     /locus_tag="VIX62_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014920886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY5859039.1"
                     /translation="MHNHGLGAWMAKRRLKSPNTTALVFDGSDVTYRQLADGADGVSA
                     LLWQRGIRRGDRVAYLGENSPEFLEVLFGAAQLGAVFVPINTRLAAPEILHVLTDSGA
                     RVLIHDPECSVRVSAAGANVSHVIPTGEGDADEPGLSQLLRLTRAGHTDAEVFLDDPA
                     AILYTSGTTGKAKGAVLTHENLTWVAINSLVDYDVVSTDVALMISPLFHAASLGMGAL
                     PVILKGATIVLEKSFDPARALELIERHGVTMLSGVPTTYQLLADHPNWASTDLSTLQK
                     LTCGGSAVPTRILNAYEERGLSFSQGYGMTETSPGATSLPPSMTRTKQGSVGLPHFFT
                     DIRIADEHGAMVPRGTVGEIEIAGPNVFPGYLNLPEANAAAFSPDGWFRSGDMGYLDA
                     DGYLYISDRLKDMIISGGENIYPAEIENLINDIDGVAGVAVIGVPDAQWGEVPWAVLT
                     VRPGASVTPETVQAHLDGKIARYKLPKNVVIVDELPRTASGKVRKADLRARFAG"
     gene            complement(2107..>2993)
                     /locus_tag="VIX62_01985"
     CDS             complement(2107..>2993)
                     /locus_tag="VIX62_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY5859040.1"
                     /translation="EEEMRSRQPKQLLLAFLGEHVVDQDELPLRASVLIEVLEGAGVA
                     APATRATLDRMVRRGLISRERRGREIRFTLTSEGSAVLREATERVRGPHPFEPHGTGW
                     TLVTFSVPENQRTLRHRLRSALTWEGFAPLRDGLWLAPGEVDLKSALEPLQADLPPGA
                     VTAFHARELPGYAMADNVRAAWDIDRIRDEHLAFIETWSDPATVAEAGTVLAAQTMLV
                     ADWLALLRADPRLPRAFMNDDWPADRSFDVYWAVRRALEEPAAAQFAALAGISLRSAP
                     ADRPSEPSLMPSAAARRR"
BASE COUNT          500 a         1102 c          951 g          440 t
ORIGIN      
        1 gtctactcct acgagggcac ccatgagatc aacgccctca tcgtcggacg atccctcacc
       61 ggcgtctcgg cattcgcctg accaccctgc aacgtccatc cacacgagga gcaacatgac
      121 caccaccgcc gcctacgccg acatcccggg cctcgcgggc accgacctcg gcttcaccga
      181 ctggctcgag atcacgcagg agagcgtcaa cacgttcgcc gacgcgaccg gcgatcacca
      241 gtggatccac gtcgaccccg agcgcgcgaa ggacggcccg ttcggcggcc cgatcgcgca
      301 cgggttcctc accctgtcgc tcgccgtgaa gttctggacc gacctgctcg acgtcgacgg
      361 tgtcggcacg aaggtcaact acggcctcga caaggtccgc ttcatctcgc ccgtcaaggt
      421 cggctcgcgc gtgcgcatga acgccgtcat cgccgaggtg accgaggtcg ccggtggctt
      481 ccagctcgcc gtcgaccaga cgatcgagat cgagggcggc accaagcccg ccgtcgtggc
      541 gcgcggcctg taccgcttct acgcctgagc tgccgcgcac ccgcgcaccc gctacccagc
      601 acgaccagca ccaccgggca cgacccgcac gaccccgcac gaaccgaacg ctcaacgaag
      661 agacgacgat gcacaatcac ggactcggcg cctggatggc caagcggcgc ctcaaatcgc
      721 ccaacacgac cgccctcgtc ttcgacggct ccgacgtgac ctatcgccag ctggcggatg
      781 gggcagacgg agtgtcggcg ctgctgtggc agcgaggcat ccgcaggggc gaccgcgtcg
      841 cctatctggg cgagaacagc cccgagttcc tcgaagtgct cttcggagcg gcgcagctcg
      901 gcgcagtctt cgtgccgatc aatacgcgcc tggccgcccc cgagatcctc catgtgctga
      961 ccgactcggg tgcgcgggtg ctcatccacg atccggagtg ctcggtgcgc gtttccgccg
     1021 ccggtgcgaa cgtctcgcac gtgatcccga ccggcgaagg cgacgccgac gagcccgggc
     1081 tctcccagct gctccggctg acccgcgcag ggcacaccga tgccgaggtc ttcctcgacg
     1141 accccgccgc gatcctctac acgtcgggca ccaccggcaa ggcgaagggt gccgtgttga
     1201 cgcacgagaa cctcacgtgg gtggcgatca acagcctcgt cgactacgac gtcgtctcga
     1261 ccgatgtcgc cctcatgatc tcgccgctct tccacgccgc atcactcggc atgggcgccc
     1321 tgcccgtcat cctcaagggc gccaccatcg tgctcgagaa gagcttcgat cctgcgcgag
     1381 ccctcgagct catcgagcgg cacggcgtga ccatgttgag cggggttccc accacgtacc
     1441 agttgctggc cgaccacccg aactgggcga gcaccgacct gagcacgctg cagaagctca
     1501 cctgcggcgg gtccgcggtg cccacacgca tcctcaacgc ctacgaggag cggggactgt
     1561 cgttctcgca gggctacggc atgaccgaga cctcgccggg cgcgacgtca ctgcccccgt
     1621 cgatgacgcg caccaagcag ggaagtgtgg gcctgccgca cttcttcacc gacatccgca
     1681 tcgctgacga gcacggagca atggtgccgc gtggcacggt gggtgagatc gagatcgccg
     1741 gcccgaacgt cttcccgggc tacctgaacc tccccgaggc gaacgcggcc gccttctcgc
     1801 ccgacggctg gttccgttcg ggcgacatgg gctacctcga tgccgacggc tacctgtaca
     1861 tctccgaccg gctgaaggac atgatcatct cgggcggcga gaacatctac ccggccgaga
     1921 tcgagaatct catcaacgac atcgacggcg tcgccggggt ggccgtgatc ggagttcccg
     1981 acgcgcaatg gggcgaagtg ccgtgggccg tgctcaccgt gcggcccggg gcatccgtca
     2041 cccctgagac cgttcaggcg cacctcgacg ggaagatcgc ccgctacaag ctgcccaaga
     2101 acgtcgtcat cgtcgacgag ctgccgcgga cggcatcagg gaaggttcgg aaggccgatc
     2161 tgcgggcgcg cttcgcaggc tgatgccggc gagcgccgcg aactgcgcgg cggcgggctc
     2221 ctcgagggct cgccgaacgg cccagtagac gtcgaacgag cgatcagccg gccaatcgtc
     2281 gttcatgaag gcgcgtggaa ggcgcgggtc cgcccggagg agcgccagcc agtccgcgac
     2341 gagcatcgtc tgcgcggcca gcaccgtgcc ggcctccgcc accgtggcag gatcgctcca
     2401 tgtctcgatg aatgcgaggt gttcatcccg gatccggtcg atgtcccacg ccgcgcgcac
     2461 gttgtcggcc atcgcgtacc ccggcagctc tcgcgcgtgg aaggcggtga cggctcccgg
     2521 cggaagatcg gcctgaagcg gctcgagcgc agacttcagg tccacctcgc cgggcgcgag
     2581 ccacagtccg tcacgtaggg gagcgaaccc ctcccacgtc agcgccgacc gcagacggtg
     2641 gcgcagcgtg cgctggttct ccggcactga gaacgtcacg agggtccacc cggtgccgtg
     2701 cggctcgaac ggatgcggcc cgcgaacgcg ctcggttgcc tcgcgcagca ccgcgctgcc
     2761 ctcgctcgtc agggtgaagc ggatctcgcg tccacggcgc tcgcgggaga tgaggccgcg
     2821 ccgcaccatc cgatcgagtg tcgccctcgt cgccggtgcc gccacccccg cgccctcgag
     2881 cacctcgatc agcacgctcg cccggagggg cagctcatcc tggtccacga cgtgctcgcc
     2941 caagaaggcc aggagcagct gcttcggctg acggcttcgc atctcttcct ctc
//
LOCUS       DATDBZ010000084         3918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2484365, whole genome shotgun sequence.
ACCESSION   DATDBZ010000084 DATDBZ010000000
VERSION     DATDBZ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484365"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VIX62_01990"
     CDS             complement(<1..1433)
                     /locus_tag="VIX62_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY5859041.1"
                     /translation="MVFLAIGGALAGVIRAELAEPGLQLVSEQTYNGVFTIHGSVMVY
                     LFAVPFGFALANYLVPLQIGAPDLAFPRLNAFSFWLYLLGGTVMLLGFVSDGGAAAFG
                     WFAYPPLSGAAGTPGIGADLWIVSVILTGTSGTLTAVNIIATITSLRAPGMTMFRMPI
                     LTWNILVTNFMVLLAFPVLSGALLMLLADRQFGTHFFDPTAGGSPILWQHLFWFFGHP
                     EVYIVALPFFGVVTEIIPVFSRRPIFGYKGLILATLAIGALSTSVWAHHMFVTGSVVL
                     PFFALTSLLIAVPTGVKVFNWIGTMWDGALTFEAPMKFALGFLITFVMGGITGVMLAS
                     PTLDFNLSDTYFVVAHFHYVMGGTVIFAVFAAIYFWWPKVTGRMLSERLATWHFWLLM
                     VGFNATFFVMHLLGRGGMPRRVADYEPYSDFKLWNMIASIGYVILVVSMVPFGVAVVK
                     SLRTPPTMGADPWEANSLEWATASPPP"
     gene            complement(1589..2263)
                     /gene="coxB"
                     /locus_tag="VIX62_01995"
     CDS             complement(1589..2263)
                     /gene="coxB"
                     /locus_tag="VIX62_01995"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY5859042.1"
                     /translation="MIAVDAPITEQAETIDRVWNLFLGLGLVVLLLVAGLMLWVIVRY
                     RRRPDTGPHELPPQKHYNIPMEITYIVIPLVIVLGLFAVTFVTVDAIDDDDPDPDLVV
                     DVIAFQWQWQFDYPDSGVSITGEPGEFPELVLPASSTVRFDLTSLDVIHSFWIPAFRF
                     KRDMIPGTPGSFRVDVADVAGYYPNTGVCAEFCGLDHSSMRFSVRILPPDEFEQWLRD
                     TQEAQS"
     gene            complement(2260..3726)
                     /locus_tag="VIX62_02000"
     CDS             complement(2260..3726)
                     /locus_tag="VIX62_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEY5859043.1"
                     /translation="MTDPTATRPQRRGLVERYVDSVTGRVGGQSWLTKNMRKVFPTHF
                     SFLWGELALYSFIVLIATGVYLTLFFQGSQDRVVYTGSYEPLRGVRVTAAFNSVMKIS
                     YDVKGGLLIRQMHHWAALIFVGAIALHMARIFFTGAFRRPREINWVVGMTLLVLALAA
                     GFTGYSLPDDLLSGTGLRITDAIILALPLVGERLSYLVFGGEWPGTDIIARLYPVHIL
                     IIPGLIIALLTIHLALVWHQKHTQFAGPGRTEGNVVGERVWPAFAMNSIGLLFLTAAV
                     ITALGAIFDINAIWLYGPYDAAAATSYSQPDWYIGFLEGSLRLFPPWETRVGGYVINN
                     VFYSGVVLPTLIFAPLFAVPWIERFLTGDSSEHHLLDRPRDAANRTAIGVGSITAVTV
                     LFLGGAQDVIANTLEMPVATVTTVLQALFLIAPPIAFWLTRRSCLALQRRPGPEHSER
                     EEEIVRTADGGYHTAQVTGGRDGPLSDDETADQETVTT"
     gene            complement(3728..>3918)
                     /locus_tag="VIX62_02005"
     CDS             complement(3728..>3918)
                     /locus_tag="VIX62_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859044.1"
                     /translation="VVRELVCPCHQSSFDPLDAAEPVAGPAPRPLPQLPLALDDEGFL
                     VAAAPFDRPVGPIAWDEG"
BASE COUNT          753 a         1221 c         1408 g          536 t
ORIGIN      
        1 tcgggcggcg gtgaagccgt ggcccattcg agcgagttcg cctcccacgg gtcggcgccc
       61 atcgtcggtg gcgtgcgcaa cgacttcacg acggccacgc cgaacggcac catcgaaacg
      121 acgaggatca cgtagccgat cgaggcgatc atgttccaca gtttgaagtc cgagtacggc
      181 tcgtagtcgg ccacccgccg gggcatgccg ccgcgaccga gcaggtgcat cacgaagaac
      241 gtggcgttga acccgaccat cagcagccag aagtgccacg tcgcgagccg ctccgacagc
      301 atccgccccg tgaccttcgg ccaccagaag tagatcgccg cgaacacggc gaagatcacg
      361 gtgccgccca tcacgtagtg gaagtgggcg acgacgaagt acgtgtcgga caggttgaag
      421 tcgagcgtcg gcgaggcgag catcacgccg gtgatcccgc ccatcacgaa cgtgatgagg
      481 aagccgagcg cgaacttcat cggtgcctcg aaggtgagcg cgccgtccca catcgtgccg
      541 atccagttga acaccttgac gcccgtcggc acggcgatca gcagcgaggt caacgcgaag
      601 aacggcagca cgaccgaccc ggtgacgaac atgtggtggg cccacaccga tgtcgacagc
      661 gcaccgatgg cgagcgtggc gaggatcagg cccttgtacc cgaagatcgg gcgtctcgag
      721 aacaccggga tgatctcggt gaccacgccg aagaacggca gggcgacgat gtacacctcc
      781 gggtgcccga agaaccagaa caggtgctgc cacaggatcg gcgagccacc ggcagtgggg
      841 tcgaagaagt gggtgccgaa ctgccggtcg gcgaggagca tcagcaacgc accggacagc
      901 acggggaagg cgagcagcac catgaagttc gtcacgagga tgttccaggt gaggatcggc
      961 atgcggaaca tggtcatgcc gggggcgcgc agcgacgtga tggtggcgat gatgttcacc
     1021 gcggtgagcg tcccggaggt gccggtgagg atcaccgaca cgatccacag gtcggcgccg
     1081 atgcccggtg tcccggcggc gcccgacagc gggggatagg cgaaccagcc gaaggcagcg
     1141 gcgccgccgt cggacacgaa cccgagcagc atcaccgtgc caccgagcag gtagagccag
     1201 aacgagaacg cgttcaggcg cgggaaggcg aggtccggcg cgccgatctg cagcggaacg
     1261 aggtagttgg cgagggcgaa cccgaacgga acggcgaaca ggtacaccat gacgctgccg
     1321 tggatggtga acacgccgtt gtaggtctgc tcgctcacca gctgcaggcc gggctcggcg
     1381 agctcggccc ggatcacgcc ggcgagcgcg ccgccgatgg cgaggaacac catcgaagcg
     1441 acgaagtagg cgacgccgag cgccttgtgg tcggtggtgg tgagccaacc cacaacgctg
     1501 cggggcagat gaccgtgttc gtgtgcgccg tggtcgatga actcctcgac cgacacgagc
     1561 ctcggtgcat cgacaggcgg atcgtcgatc acgattgggc ctcctgcgtg tcgcgcagcc
     1621 actgctcgaa ctcgtcgggc ggcaggatcc gcaccgagaa gcgcatcgag gagtggtcga
     1681 ggccgcagaa ctcggcgcac acgcccgtgt tcgggtagta gccggcgacg tcggcgacgt
     1741 cgacgcggaa cgacccggga gtgccgggga tcatgtcgcg cttgaagcgg aacgccggga
     1801 tccagaacga atggatcacg tcgagggagg tcagatcgaa acgcacggtc gacgacgcgg
     1861 gcaacacgag ttcggggaac tcgccaggct cgccggtgat gctcaccccg ctgtcggggt
     1921 agtcgaactg ccactgccac tggaacgcga tgacgtcgac cacgaggtcg ggatccggat
     1981 cgtcgtcgtc gatcgcgtcg acggtcacga acgtcacggc gaacagaccg agcacgatca
     2041 cgagcgggat cacgatgtac gtgatctcca tcgggatgtt gtagtgcttc tgcgggggca
     2101 gttcgtgtgg accggtgtcg gggcgtcggc ggtaccgcac gatcacccac agcatcaggc
     2161 cggccacgag cagcagcacg acgagcccga gaccgaggaa caggttccac acccggtcga
     2221 tcgtctcggc ctgctcggtg atcggggcgt cgacggcgat catgtcgtca ccgtctcctg
     2281 gtcggccgtc tcgtcgtcgc tcaggggccc gtcccggccc cccgtcacct gggcggtgtg
     2341 gtagccgccg tcggcggtgc gcacgatctc ctcctcgcgc tcggagtgtt cggggcccgg
     2401 ccgccgctgc agcgcgaggc acgagcggcg agtgagccag aacgcgatcg gtggggcgat
     2461 caggaacagc gcctggagca ccgtggtcac ggtggcgacg ggcatctcga gggtgttggc
     2521 gatcacgtcc tgggcgccgc cgaggaagag cacggtgacc gcggtgatcg acccgacccc
     2581 gatcgccgtc cggttcgccg cgtcgcgagg ccggtcgagc aggtggtgct cggacgagtc
     2641 gccggtgagg aatcgttcga tccacggcac ggcgaacagt ggggcgaaga tcaatgtcgg
     2701 caggacgacg cccgagtaga agacgttgtt gatcacgtac ccgccgaccc gcgtctccca
     2761 cggtgggaac agccggagcg agccttcgag gaacccgatg taccagtcgg gttgcgagta
     2821 ggacgtggcc gcggcggcgt cgtacgggcc gtacaaccag atcgcgttga tgtcgaagat
     2881 cgcgccgagc gcggtgatca cggcggcggt caggaacagc aggccgatcg agttcatcgc
     2941 gaacgccggc cacacccgct cgccgaccac gttgccctcc gtgcgtcccg gcccggcgaa
     3001 ctgggtgtgc ttctggtgcc acacgagcgc gagatggatc gtcagcaggg cgatgatgag
     3061 accggggatg atcaggatgt gcaccggata gagccgggcg atgatgtcgg tgccgggcca
     3121 ctccccaccg aacacgaggt acgacaggcg ctcgccgacg agcgggaggg cgaggatgat
     3181 ggcgtcggtg atgcgcaggc cggtgcccga cagcaggtcg tcggggagcg agtagccggt
     3241 gaaccccgcg gcgagcgcga ggacgagcag ggtcatgccg acgacccagt tgatctcgcg
     3301 cggtcgacgg aacgcaccgg tgaagaagat ccgggccatg tgcaacgcga tcgcaccgac
     3361 gaagatcagc gctgcccagt gatgcatctg gcggatgagc agcccgccct tcacgtcgta
     3421 ggagatcttc atcaccgagt tgaacgccgc cgtcacccgg accccgcgga gcggttcgta
     3481 gctgccggtg tacacgacgc ggtcctgcga cccctggaag aacagggtga gatagacgcc
     3541 cgtcgcgatc agcacgatga agctgtagag ggcgagttcg ccccacagga acgagaagtg
     3601 ggtggggaac accttgcgca tgttcttcgt gagccacgat tggccgccga cgcggccggt
     3661 gaccgagtcg acgtagcgct cgaccagtcc ccgccgctgc ggtcgcgtcg cggtgggatc
     3721 cgtcatctca gccctcgtcc caggcgatcg ggccgaccgg ccgatcgaag ggcgcagcgg
     3781 caacgaggaa gccctcgtca tcgagagcca gcgggagttg cgggagcgga cgcggcgcgg
     3841 gcccggcgac gggttcggcc gcgtcgagcg gatcgaacga cgactggtgg cacgggcaca
     3901 cgagttcgcg cacgacct
//
LOCUS       DATDBZ010000085         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_435417, whole genome shotgun sequence.
ACCESSION   DATDBZ010000085 DATDBZ010000000
VERSION     DATDBZ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435417"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VIX62_02010"
     CDS             complement(<1..256)
                     /locus_tag="VIX62_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859045.1"
                     /translation="MPIVRVAFLQDLTVPSHVDLVSPSFLAFDLALHDAVETGGLDID
                     VEVVQMDTDGDPERAAAFAEEIAADPSYVAAVAAPFWTEPA"
     gene            381..464
                     /locus_tag="VIX62_02015"
     tRNA            381..464
                     /locus_tag="VIX62_02015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:415..417,aa:Leu,seq:caa)
     gene            complement(487..1800)
                     /locus_tag="VIX62_02020"
     CDS             complement(487..1800)
                     /locus_tag="VIX62_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5859046.1"
                     /translation="MTRTGATCRVLVVLLAVSTSACFGAGAQDPPDSATTTPEDPLGT
                     ITIEPGAPIELATMLAGASGPGSTTGVDAVRGVRLAVDFLDGKLDGSSGPLLNHPVEL
                     VEIPETCEGGRSVETAVPDVDVDVVGVIGPGCARSVRAEPATSLAERGVVMISPTATE
                     AELTDPSQRPPTFFRTAYNDLVEGKAVADYAAGELEALRSGVATDEAGSTGAASSFRS
                     TFEEDLGIVAVSGTLDGPADASRLVRAIALGRPEFVFLVGRRPACADAARESGELPAL
                     RAVPVVTSVGCFDPAFVAVDAASPLQYLSGPDLTQMLEDDFYRMEFLPAYQEQFGSSP
                     VGPFHAHAYDATLMLLDAIDRIAEQSEDGTLTIGRTALQEAVAATSGVSGISGMITCS
                     ETGDCAPDPTIAVYLVPDVPLAGGSLDAVPVFSETVALADLVTPA"
     gene            complement(1803..>2072)
                     /locus_tag="VIX62_02025"
     CDS             complement(1803..>2072)
                     /locus_tag="VIX62_02025"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY5859047.1"
                     /translation="CVATYGVTGAREELSDTARSLVGALRPHTDLPLLAGVGIATPEQ
                     AAEACVFADGVIVGSALMALMVDGRRDDMLALAASFHRATRIDEA"
BASE COUNT          357 a          746 c          676 g          293 t
ORIGIN      
        1 ccgccggttc ggtccagaac ggtgccgcga ccgccgcgac gtacgagggg tcggcggcga
       61 tctcctctgc gaaggccgcg gctcgttcgg gatctccgtc ggtgtccatc tggaccacct
      121 cgacatcgat atcgagaccg cccgtctcga ccgcgtcgtg cagagccagg tcgaacgcca
      181 agaatgaggg agacaccagg tcgacgtgcg agggaaccgt caggtcctgc aggaaggcga
      241 cccgcacgat cggcatgtct gcccccccgg ctcccgtcga cgaacacccc gccaggagcc
      301 cgaagcaggt gatgaacgcg acgagagcgg cgcctcgagg cccagacgtg atccctcgcc
      361 cgctatcgta tggggctccg gccgggatgg cggaactggc agacgcgcgg gactcaaaat
      421 cccgtgagct tcggctcgtg agggttcgac cccctctccc ggcaccgagt gaggtccacc
      481 tcgacgtcac gccggggtca ccaagtcggc cagcgccacc gtttcggaga agacaggcac
      541 ggcatcgagc gatccgcccg caagcggcac gtccgggacc aggtagacag cgatcgtggg
      601 gtcgggcgcg cagtctccgg tctcgctaca tgtgatcatg ccggagatcc ccgacacccc
      661 gctggtcgcc gccaccgcct cctgcaatgc cgtccgtccg atcgtgagcg tgccgtcttc
      721 cgactgctcg gcgatccggt cgatcgcatc caggagcatc aaggtggcgt cataggcatg
      781 ggcatggaac ggaccgaccg gcgaggaacc gaactgctcc tgataggccg gcaggaactc
      841 catgcggtag aagtcatcct cgagcatctg cgtgagatcg ggacctgaca ggtactgcag
      901 cggcgatgcc gcatcgaccg ccacgaacgc tgggtcgaag caccccaccg aggtgacgac
      961 cgggacagct cgcagggctg ggagctcgcc cgactcccga gctgcatcag cgcacgcagg
     1021 ccgccttccg accaggaaca cgaactccgg gcggccgagt gcgatcgctc gaaccagccg
     1081 agaggcgtcg gctggcccat ccagggtgcc cgacaccgcg acgatcccta ggtcctcctc
     1141 gaacgtcgag cggaacgacg acgcagctcc cgtggaaccg gcctcgtcgg tcgcgacgcc
     1201 actacggagc gcctcgagtt cgccggccgc gtagtcggcc accgccttcc cttcgacgag
     1261 gtcgttgtac gccgtcctga agaacgtcgg aggtcgctgc gagggatcgg tgagctcggc
     1321 ctcggtggcc gttggcgaga tcatgacgac accacgctcc gcgaggctcg tggcgggctc
     1381 cgccctgacc gaccgcgcac acccgggccc gatcacgccg accacgtcca catcgacgtc
     1441 cgggaccgct gtctcgaccg atctgccgcc ctcgcacgtc tccgggatct ccaccaactc
     1501 gaccggatga ttcaagaggg gacccgaact tccgtcgagc ttcccgtcca ggaagtccac
     1561 cgccaggcgg acacctcgga cggcgtccac ccccgtcgtc gagcccgggc cggaggcgcc
     1621 cgccaacatc gtcgccagtt cgatgggcgc gccgggctcg atcgtgatcg tgccgagcgg
     1681 gtcctcgggc gtcgtcgtgg cgctgtcagg gggatcctgc gcaccggctc cgaagcacgc
     1741 tgaggtgctg acggcgagca gcaccaccag cacccgacac gtggctccgg tccgcgtcac
     1801 ggtcaggcct cgtcgatgcg cgtggcgcgg tggaacgagg cagccagtgc caacatgtcg
     1861 tcacgtcggc cgtccaccat gagcgccatc aatgcgctcc ccacgatcac cccgtccgcg
     1921 aacacgcatg cctcggcagc ctgctcaggt gtcgcgatcc cgacgcccgc gagcagcggc
     1981 aagtccgtat gtggacgaag cgccccgacg agcgaacgcg cggtgtcgga cagttcttcg
     2041 cgggcgcccg tcacgccata cgttgcgacg ca
//
LOCUS       DATDBZ010000086         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_435459, whole genome shotgun sequence.
ACCESSION   DATDBZ010000086 DATDBZ010000000
VERSION     DATDBZ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435459"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VIX62_02030"
     CDS             complement(<1..916)
                     /locus_tag="VIX62_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY5859048.1"
                     /translation="MVFQGSSVAISHTGKVRSNNQDSGYCGSNLFAVADGMGGHAGGD
                     VASSLAIARLERLDHPFESTSAAERELRDAIADSAAELIDTVHVKPELAGMGTTVSAL
                     VMVDDYAVIAHIGDSRIYLYRDEALTQITTDHTFVQRLVDSGRITPEEARFHPRRSVL
                     MRVLGDMDPNPELDTFIMPTQPGDRWLLCSDGLSGVVDDAHTAKALGLGLAPGRTADN
                     LLKQALDAGAPDNVTIVLVDVGGQHPIFSGTPTIVGSASNPLGVEVPAARSGRGNWLH
                     PNRQAANEPSHFEPAAEFLEELIEEDRRR"
     gene            complement(931..1458)
                     /locus_tag="VIX62_02035"
     CDS             complement(931..1458)
                     /locus_tag="VIX62_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5859049.1"
                     /translation="MSELTLLLLRIGFLVLLWFFVFAVVYSLRADLFGVKVRRMPEPA
                     PAAAPAPAARASATAPVASSRPTSSGAKGGAATTDTVSRIVITSGPKAGLELPLGSEP
                     LTIGRSSESGLVIRDDYTSSHHARLVLWGEQWMIQDLDSTNGTWHDGARVSAPMPITV
                     GAPIKVGATTFELRK"
     gene            complement(1455..2192)
                     /locus_tag="VIX62_02040"
     CDS             complement(1455..2192)
                     /locus_tag="VIX62_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEY5859050.1"
                     /translation="MGLLDSFEKGLERAVNSAFAKTFRSGIQPVEIASALRSELDKKA
                     AIVSRDRILTPNTFTVHLSTADDERMRTLGGTLGEELDTLVQAHAKAQGYSFAGPVSI
                     TLVRDDQLATGTLRVESATAEGRVAWRGVLEVEGKRHPIVKSRTVIGRGSDADITISD
                     AGTSRKHVEILWDGERAMVRDMQSTNGTMLNGRKVTEAALAPDSTVTIGRTDIVFRVV
                     AQAAPPKPPMPADATRAYNVREGGPLR"
     gene            2342..2425
                     /locus_tag="VIX62_02045"
     tRNA            2342..2425
                     /locus_tag="VIX62_02045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2376..2378,aa:Leu,seq:cag)
BASE COUNT          369 a          820 c          891 g          418 t
ORIGIN      
        1 cgcggcggcg gtcctcttcg atgagctcct cgaggaactc cgcggcgggc tcgaagtggc
       61 tgggctcgtt ggccgcctgc cggttggggt gcagccagtt gccacggccg gaacgcgcgg
      121 ccggcacttc gacacccagc gggttcgagg ccgatcccac gatcgtggga gtgccggaga
      181 agatcggatg ctgcccaccg acgtccacca ggacgatggt gacgttgtcg ggggccccgg
      241 catccagcgc ctgcttgaga aggttgtcgg ccgtgcggcc cggcgcaagg ccgagtccca
      301 gtgccttcgc ggtgtgcgca tcgtcgacca cccccgacag accgtcggag cacagcagcc
      361 atcggtcgcc cggctgggtc ggcatgatga aggtgtccag ctcggggttc gggtccatgt
      421 cgccgagcac gcgcatcagc accgatcggc gcgggtggaa cctcgcttct tccggggtga
      481 tgcgtcccga gtcgacgaga cgctgcacga acgtgtgatc cgtcgtgatc tgggtgaggg
      541 cctcgtcgcg gtacaggtag atgcgcgagt cgccgatgtg cgcgatgacc gcatagtcgt
      601 ccaccatgac gagtgcgctg acagtggttc ccatgccggc cagctcgggc ttgacgtgca
      661 cggtgtcgat cagctcggcg gccgagtcgg cgatcgcgtc gcgcagctcc cgttcggcgg
      721 cggaggtgga ctcgaacgga tggtcgagcc gctcgagtcg ggcgatcgcg aggctggatg
      781 ccacatctcc accggcgtgc ccgcccatgc catccgcgac cgcgaacagg ttggaaccgc
      841 aatagccgga gtcctggttg ttggagcgca ccttgccggt gtgcgagatc gcgacgctcg
      901 agccctggaa gaccatatgt cccgggtccg ctacttccgc agctcgaagg tcgtcgcccc
      961 gaccttgatc ggcgcaccga ccgtgatggg catcggcgcg ctcacccgcg cgccgtcgtg
     1021 ccacgtgccg ttggtcgagt cgaggtcctg gatcatccac tgctcgcccc acagcacgag
     1081 gcgggcgtgg tggctggagg tgtagtcgtc gcggatcacg aggccggact cgctggatcg
     1141 tccgatcgtg agcggctcgg agcccagcgg cagctcgagg ccggctttcg ggccgctcgt
     1201 gatgacgatc cgcgacacgg tgtcggtcgt ggccgctccg cccttcgcac cggacgacgt
     1261 gggccgcgac gatgccacgg gggcggtcgc cgaagcccgg gccgcggggg ccggcgccgc
     1321 cgcaggcgcg ggttccggca tccgtcgcac cttgacgccg aacagatcgg cacgcaaaga
     1381 gtagacgacc gcgaagacga agaaccacag cagcacgagg aacccgatgc gcagcagcag
     1441 gagggtcagt tcactcaacg cagcggccca ccttcgcgga cgttgtacgc ccgcgtggcg
     1501 tcggccggca tcggaggctt gggcggggcg gcctgcgcga cgacccggaa cacgatgtcg
     1561 gtgcgcccga tcgtcacggt cgaatccggc gcgagcgcgg cctccgtgac cttgcgcccg
     1621 ttcagcatgg tgccgttggt ggactgcatg tcgcgcacca tcgcccgctc gccgtcccac
     1681 aggatctcga cgtgcttgcg gcttgtgccc gcgtcggaga tcgtgatgtc ggcgtcgctg
     1741 ccacggccga tcacggtccg cgacttcacg atcggatgcc gcttgccctc gacctcgagc
     1801 acgccgcgcc acgcgacgcg gccctcggcg gtggccgact ccacgcggag cgtgccggtc
     1861 gcgagctgat cgtcgcgcac cagggtgatc gagaccgggc cggcgaacga gtagccctgc
     1921 gccttggcgt gtgcctgcac gagggtgtcg agctcttcgc cgagcgtgcc gccgagcgtg
     1981 cgcatgcgct cgtcgtcggc cgtcgacagg tgcaccgtga acgtattcgg cgtcagaatg
     2041 cggtcgcggc tcacgatggc ggccttcttg tcgagctcgc tccgcagggc ggatgcgatc
     2101 tcgacgggtt ggatgccgct gcggaaggtc ttggcgaacg cgctgttgac ggcgcgctcg
     2161 agacccttct cgaagctgtc aagtaatccc acgcgactcc tcaggcaggt ggtgccggtc
     2221 ggtacatgct aattgcttag tctgtgttgc ccgtggatca ccggtgcacg ccgattcgag
     2281 cggcgggatg ctgcgtgata tcctcgggaa gttgagttgg cgtcttcgga tgccgcatct
     2341 cgcgcgagtg gcggaatagg cagacgcgct ggcttcaggt gccagtgtcc gaaaggacgt
     2401 gggggttcaa ctcccccctc gcgcacgaaa cgaccaatgg gctcccgaag ggggcccatt
     2461 cgtcgttcta tggccgcagg gttactccag cgccggga
//
LOCUS       DATDBZ010000087         7023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2075965, whole genome shotgun sequence.
ACCESSION   DATDBZ010000087 DATDBZ010000000
VERSION     DATDBZ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2075965"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /gene="trpD"
                     /locus_tag="VIX62_02050"
     CDS             complement(<1..738)
                     /gene="trpD"
                     /locus_tag="VIX62_02050"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY5859051.1"
                     /translation="MTESDANFWPHTLLRLSAREALSADDAAEVMRRIMAGEASAGQI
                     GGFLMALRTKGETADEVEGLARTMLSFATPVASPTPTVDVVGTGGDRSGTFNISTVAA
                     IVTAAAGAVVAKHGNRAASSHCGSADVLEALGVRIDLDASGVERCLADAGIAFLFAPM
                     FHPSMAHAGPVRGELRVPTVFNFLGPLTNPARPAAQAVGCSDARMMPLMAEVLARRGT
                     RAMLFRGDDGLDELTTTGPSTVLDVADG"
     gene            833..906
                     /locus_tag="VIX62_02055"
     tRNA            833..906
                     /locus_tag="VIX62_02055"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:865..867,aa:Gly,seq:ccc)
     gene            complement(923..1627)
                     /gene="nth"
                     /locus_tag="VIX62_02060"
     CDS             complement(923..1627)
                     /gene="nth"
                     /locus_tag="VIX62_02060"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY5859052.1"
                     /translation="MSPTRTEPADWTPPDRRRIRAIYRRLDTYFEGELAPPRAADPLD
                     ELILTVLSQHTSDINAERAFADLQRVFPGGWQQVVDAPTVGVADAIRSGGLANSKAPR
                     IQAILREVRDREGSFDLSRLRAMSDAEARDYLIGLPGIGPKTAAVVLSFALGRDAMPV
                     DTHVHRVTKRLGLIPPKATAERADRILHELVPDGMRTPLHVAFIRLGREICKAPTPRC
                     RQCPLKELCPTAPRYL"
     gene            complement(1624..2988)
                     /gene="dnaB"
                     /locus_tag="VIX62_02065"
     CDS             complement(1624..2988)
                     /gene="dnaB"
                     /locus_tag="VIX62_02065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY5859053.1"
                     /translation="MARTADELRTVSLDDRRVPPQNLEAEESVLGSMMLSAEAIADVV
                     EILDIEDFYRSANAKIYSTLRGLYARGEPVDIITGIEALRADGLLDEVGGQLYLRDLV
                     DQVPTPASAGHYAQIVARSALRRRLISAAADIMDLAYASTEDADVVADSAEQRIYDVA
                     RREDHEDVAVLRELVNQAMVDLESIQNRDSAFTGLPTGFVDLDNLMSGLQPGNLIVIA
                     ARPGIGKSSLAMNMARNIAVEGAPVAMFSLEMSRMEIGMRLLCAEARVPWDRIRNKRV
                     GPDDWQRVVHAAEILHDAPLSIVDSGNVNIVDIRAKARRMRTGNQGLSLIIVDYLQLM
                     TSPNARRPDSRQQEVAEISRNLKLLAKELHVPVVALSQLNRNPEARADKRPQLSDLRE
                     SGAIEQDSDVVMFIHRDDADPEKKKHAELIVAKHRNGPTDTIRLSFEPSLTQFRNAAR
                     DMGA"
     gene            3237..3815
                     /locus_tag="VIX62_02070"
     CDS             3237..3815
                     /locus_tag="VIX62_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5859054.1"
                     /translation="MVHEASTHRSSRRSDLAAMRALAHPIRVRMVVLLRGEALSASDL
                     ARRLDLRFGSARFHLHKLVDADIAVPAGERVDRGGTALLFRVPEGLWIDLDADAPPEL
                     NVAIRRNLVRELERRLDAAALDQRPDDTVHDVLSLRELAIRAPDRAEAETIVEEALAR
                     LIRLHDPDAPDAEPHTIGLFSFRTPRTETTEP"
     gene            3812..5065
                     /locus_tag="VIX62_02075"
     CDS             3812..5065
                     /locus_tag="VIX62_02075"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859055.1"
                     /translation="MTAARANVLRSNRVFRRMWSARAISFIGDGIALTALVIYIQDTR
                     GTGTAVAALLLAQALPHLLGPVAGTVADRVDQRTLMIGCDLGRAVLFGAAAWFLPSMA
                     WLLVVMAAASTLDTLFAPAGRSAVPALVEDADLVNANAWLGTALNLQVALGPLIGGAL
                     VAVIDVRGALAVDAGSFLLSALLLLGVPKLLPSVRRADRERFIPEMRAGLAYARSHPV
                     ARAVVVTLFLGVAFAGIDNVALVFLARDQLGAGPAGFGAVASAFGVGMLVASVMLSRR
                     ASSITPRALFIGGWAMTGLGTLLTAVAPAVWFAVAAQAVSGAGNGADNVASDSLLQRS
                     VPRAMLGRVFGLTSTAAFVGGGLAYAAGGPLLDATSARTVFLIGAAGTFAVVLLATRL
                     LPRSDPEDEPREPDASDTGPGGTPR"
     gene            complement(5254..5706)
                     /gene="rplI"
                     /locus_tag="VIX62_02080"
     CDS             complement(5254..5706)
                     /gene="rplI"
                     /locus_tag="VIX62_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY5859056.1"
                     /translation="MKVILQKPVDKLGVPGDVADVADGYARNYLIPQGMAVKASKNMV
                     NHADSLRRAHEKRRYAMKGEYEAQASKVISGGPIRVSAKAGEEGKLFGSVTAEHIAAA
                     IKAQSDVDIDRRDVRLDEPIRSIGTHEVKIHLFTDVEPIITVEVDPAG"
     gene            complement(5703..5978)
                     /gene="rpsR"
                     /locus_tag="VIX62_02085"
     CDS             complement(5703..5978)
                     /gene="rpsR"
                     /locus_tag="VIX62_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY5859057.1"
                     /translation="MPAKGKGGPKRRKEKDEKAWQKKGKRKFCIFCKDRVDYIDYKDT
                     ITLRKFVSERGKLRARRVTGNCVQHQRDVASAVKNAREMALMTYSSR"
     gene            complement(5968..6432)
                     /gene="ssb"
                     /locus_tag="VIX62_02090"
     CDS             complement(5968..6432)
                     /gene="ssb"
                     /locus_tag="VIX62_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY5859058.1"
                     /translation="MASDNQVTLVGNLTDDPELRYTPNGAAVCKFRIAVNRRIPDGAG
                     GWKDAEASYFSVNCWRSLGENVAESLTRGSRVVVAGRLNYRSWENQDGEKRNAIEIEA
                     DEVGPSLRWATARVERQSRSTSASSSGDWGEKVAAPVGGGGTDQEENPLDAG"
     gene            complement(6466..6828)
                     /gene="rpsF"
                     /locus_tag="VIX62_02095"
     CDS             complement(6466..6828)
                     /gene="rpsF"
                     /locus_tag="VIX62_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY5859059.1"
                     /translation="MREYEIMLILPAEADESVVSTAMDRITKVISASGGEVSNIDRWG
                     RRRFAYELKHLTEGYYVVAKFTAQPETQAELDRVLNLADEVIRFKVLVLPEQRANSRQ
                     NRRDQKEAASSPVPAETA"
BASE COUNT         1002 a         2384 c         2415 g         1222 t
ORIGIN      
        1 gccgtcggcg acgtccaaca cggtggaggg gccggtcgtg gtgagctcgt cgagaccgtc
       61 atcgccgcgg aagagcatcg cgcgggtgcc acgcctcgcc agcacctcgg ccatcaacgg
      121 catcatgcgg gcatccgaac agccgaccgc ctgggcggcc ggacgtgccg ggttcgtgag
      181 tgggcccagg aagttgaaca cggtgggcac ccggagctcg cctcgcaccg gcccggcgtg
      241 ggccatggac ggatggaaca tcggcgcgaa caggaaggcg atgccggcat cggccaggca
      301 ccgttccact ccggaggcgt cgaggtcgat cctcacgccg agcgcctcga gcacgtcggc
      361 cgacccgcag tggctcgaag ctgcacggtt cccgtgcttg gcgacgaccg ctccggcggc
      421 cgccgtcacg atcgccgcca ccgtggagat gttgaacgtg ccgctgcggt cgccgccggt
      481 gcccacgacg tcgaccgtcg gcgtcggtga cgccaccggt gtcgcgaacg acagcatcgt
      541 gcgggccagg ccttcgacct cgtcggccgt ttcgcccttg gtccgcaacg ccatgaggaa
      601 cccgccgatc tggccggccg aggcttcccc cgccatgatc cggcgcatca cctcggccgc
      661 atcgtcggcg ctcaaggcct cgcgcgcgga caggcgcagg agggtgtgcg gccagaagtt
      721 ggcgtcgctt tcggtcatgg ttcgggcaag ctagcaggtc gtgtcgggct cgcgcgaggg
      781 caccgcggct cgctacactg tcggcttcac gccgcacgaa cgggtgtgag atgcgggtgt
      841 ggttcaatgg tagaacgcaa gcttcccaag cttgaaacgg gggttcgatt cccctcaccc
      901 gctccaacgg gtcccgcgaa cctcacaggt atcgcggcgc cgtcggacag agttccttca
      961 acgggcactg ccggcatcgc ggcgtgggcg ccttgcagat ctcccgtccc agccggatga
     1021 acgccacgtg cagcggcgtg cgcatcccgt cgggcaccag ctcgtgcaag atgcgatcgg
     1081 cgcgttccgc ggtggccttc ggagggatca accccagccg cttggtcact cgatgcacgt
     1141 gcgtatcgac gggcatcgcg tcgcggccga gcgcgaagct gagcaccacc gccgcggtct
     1201 tcggcccgat gccgggcaag ccgatcaggt agtcgcgggc ctcggcgtcg ctcatggcgc
     1261 gcagcctcga gaggtcgaac gacccctcgc gatcgcgcac ctcgcgcagg atcgcttgga
     1321 tccggggagc cttcgagttg gcgagtccgc cgctccggat cgcatcggcc accccgacgg
     1381 tcggcgcgtc cacgacctgt tgccacccgc cggggaacac ccgctgcaga tcggcgaacg
     1441 cccgctcggc gttgatgtcg ctcgtgtgct gtgacagcac ggtcaagatc agctcgtcga
     1501 gcggatcggc ggcgcgaggc ggcgcgagct cgccctcgaa gtaggtgtcg agccgccggt
     1561 agatggctcg gatgcgccgg cggtcgggag gggtccagtc ggcgggttcg gtacgggtgg
     1621 ggctcacgcg cccatgtcgc gcgcggcgtt ccggaactgg gtgagcgacg gttcgaagct
     1681 gagccggatg gtgtcggtgg gtccgttgcg gtgcttggcg acgatgagct cggcgtgctt
     1741 cttcttctct gggtcggcat cgtcacggtg gatgaacatg acgacgtcgc tgtcctgttc
     1801 gatggcgccc gactcgcgga ggtcggacag ctggggtcgc ttgtcggcgc gcgcctccgg
     1861 gttgcggttg agctgggaga gcgccaccac gggcacatgc agttccttgg ccaggagctt
     1921 gaggttccgg ctgatctcgg cgacctcctg ctgacggctg tcgggccggc gggcgttggg
     1981 gctggtcatg agctggagat agtcgacgat gatgagcgag agcccctggt tgcccgtgcg
     2041 catgcgccgg gccttggcgc ggatgtcgac gatgttgacg ttgcccgagt cgacgatgga
     2101 cagcggggca tcgtgcagga tctcggcggc atgcaccacc cgttgccagt cgtcggggcc
     2161 gacgcgcttg ttgcggatcc gatcccacgg cacacgagcc tcggcgcaca gcagtcgcat
     2221 gccgatctcc atgcgcgaca tctccagcga gaacatcgcc acgggtgcac cttcgaccgc
     2281 gatgttgcgc gccatgttca tggccagcga actcttgccg atgcccggcc gcgcagcgat
     2341 gacgatgagg ttgccgggct ggaggcccga catcaggttg tcgaggtcga cgaagcctgt
     2401 cggcagcccc gtgaacgccg agtctcggtt ctggatggac tcgaggtcca ccatcgcctg
     2461 gttgaccagc tcgcgcagca ccgcgacgtc ctcgtgatcc tcgcggcggg ccacgtcgta
     2521 gatccgctgc tcggcactgt cggcgaccac gtcggcgtcc tcggtgctgg cgtaggcgag
     2581 atccatgatg tcggcggcgg ccgagatgag ccggcggcga agggcggagc gcgccacgat
     2641 ctgcgcgtag tggcccgccg aggccggtgt ggggacctgg tcgacgagat ctcgcagata
     2701 gagctgcccg cccacctcgt cgagcagacc gtcggcgcgc aacgcctcga taccggtgat
     2761 gatgtcgacg ggctcgcctc gcgcgtacag gcctcggagc gtggagtaga tcttggcgtt
     2821 ggccgagcga tagaagtcct cgatgtccaa gatctcgacg acgtcggcga tggcctcggc
     2881 cgagagcatc atggatccca gcaccgattc ctcggcctcg aggttctgcg gcgggacgcg
     2941 gcggtcgtcc agggatacgg ttcggagttc gtcagccgtg cgtgccatgg ccgtgcaccc
     3001 ctccccctgt cgacgccggg cccctcaacc ccgggacgct acgacgcatg aggtagctcc
     3061 gacagttgat aacccctgtg gattgcagat ccgcaggtcg cgagccgaga ttccttgtca
     3121 ctccggccct gacctgcgct ttccacatgt ggaaacccat gtggacaagt gtgaatcaca
     3181 ccagtgacat tcgaaggcgg ccgccaacca tataacactt gttgtagggt gaatcgatgg
     3241 tccacgaagc gagcacccac cggtcgtcac gtcgttcgga tctggcggcg atgcgagcgt
     3301 tggcgcaccc gatccgggtc cgcatggtgg tgctgctgcg gggcgaggcc ctgtctgcct
     3361 cggatctggc tcgacgactc gaccttcgct tcggcagcgc tcggttccac ctgcacaagc
     3421 tggtcgatgc ggacatcgcc gtccccgccg gagagcgggt cgatcgaggc ggcaccgcgc
     3481 tgctgttccg cgtgcccgaa ggactgtgga tcgacctcga cgccgacgcg ccccccgagt
     3541 tgaacgtcgc gatccgccgg aacctcgtgc gcgaactcga acgtcgtctg gacgccgccg
     3601 ccctcgacca gcgcccggac gacacggtcc acgacgtcct gtcgctgcgg gagctcgcga
     3661 tccgggcgcc cgaccgcgcg gaggcggaga cgatcgtcga ggaggccctg gctcgcctga
     3721 tccgtctgca cgacccggac gccccggacg ccgaaccgca cacgatcggc ctgttctcgt
     3781 tccggacccc ccgcacggaa acgaccgaac catgacggct gcgcgagcca acgtcctgcg
     3841 ctcgaaccgg gtgttccggc ggatgtggag cgctcgagcg atctccttca tcggtgacgg
     3901 gatcgcgctc accgccctgg tcatctacat ccaggacaca cgcgggacgg gaaccgcggt
     3961 ggcagcgttg ctgttggccc aggcgctgcc gcacctgctg ggtccggtcg ccggcaccgt
     4021 ggcggatcgt gtggatcagc gcacgctgat gatcgggtgc gacctggggc gggccgtgct
     4081 gttcggtgcc gcggcctggt tcctgccttc gatggcctgg ctcctcgtgg tgatggcggc
     4141 ggcgtccacg ctcgacacgt tgttcgcgcc cgctggccgc agcgcggtcc ccgccctggt
     4201 cgaggacgcc gacctggtga acgcgaacgc gtggctcggc accgcgctca acctccaggt
     4261 ggccctcggc ccactgatcg gtggggcgct ggtggccgtc atcgatgtgc gaggcgcatt
     4321 ggcggtggac gcggggagtt tcctgctctc ggcgctcctg ctgctcggtg tgccgaagct
     4381 cctgccgtcg gtgcgccggg ccgaccgcga acggttcata ccggagatgc gggcaggtct
     4441 ggcctatgcg cgatcccatc ccgtcgcccg ggccgtcgtg gtgaccttgt tcctcggggt
     4501 ggcgttcgcg gggatcgaca acgtcgcact ggtcttcctg gctcgcgacc aactcggggc
     4561 gggaccggcc gggttcggcg ccgtggcgtc ggccttcggc gtcggcatgt tggtggcctc
     4621 cgtcatgctg agccgtcggg cctcgagcat cacgccacgc gcactcttca tcggtggctg
     4681 ggcgatgacc gggctgggca ccctcctgac agccgtcgct cccgcggtgt ggttcgccgt
     4741 ggcggcccag gcggtgagcg gagccggcaa cggcgccgac aacgtggcct ccgactcctt
     4801 gctccaacga tcggtccccc gagcgatgct cggccgcgtc ttcggcctga cctccaccgc
     4861 agccttcgtc ggcggaggcc tcgcctacgc cgccggcggc cccctcctcg acgccacctc
     4921 agcccgaacc gtcttcctca tcggagcagc gggcaccttc gcagtcgtcc tcctcgcaac
     4981 ccgcctcctt ccaagatccg atcctgagga cgagccacga gaaccagatg cgtctgacac
     5041 agggcccggg ggcacaccgc gctgaggaac gcctcctcgc ggcgaccccc gggcccgttc
     5101 cagaagcttc gggacgcgct caccgcgatc cggacggcca agcgtgacgg agacgtgcga
     5161 aggcccccgg ggtcagcgac tagggcgttt ctttccctgg gcgctgtgcc ccgggggcct
     5221 cacgcgagac caccgggtct tgcttcgctg ctcttagcct gcggggtcga cctcgaccgt
     5281 gatgatgggc tcgacgtcgg tgaacaggtg gatcttcacc tcgtgggtgc cgatcgagcg
     5341 gatgggctcg tcgagccgta cgtcacgcct gtcgatgtcg acgtcggact gggccttgat
     5401 cgccgcggcg atgtgctcgg cggtgaccga cccgaacagc ttgccttcct cgccggcctt
     5461 ggccgagacg cggatggggc cgcccgagat caccttggat gcctgggctt cgtattcgcc
     5521 cttcatggcg tagcggcgct tctcgtgggc acggcggagg ctgtcggcgt ggttgaccat
     5581 gttcttgctg gccttgaccg ccatgccctg ggggatcagg tagttgcgcg cgtagccgtc
     5641 ggcaacatcg gcgacatcgc cgggcacacc gagcttgtcg acgggcttct ggaggatgac
     5701 cttcatcggc tcgagtaggt catcagcgcc atctcgcgcg cgttcttgac cgccgatgcg
     5761 acgtcgcgct gatgctgcac gcagttgccc gtgacgcggc gagcacgcag cttgccccgc
     5821 tccgagacga acttgcgcag cgtgatggtg tccttgtagt cgatgtagtc gacgcggtcc
     5881 ttgcagaaga tgcagaactt gcgcttgccc ttcttctgcc aggccttctc gtccttttcc
     5941 ttgcggcgct tcgggccgcc ttttccttta gccggcatcc agtgggttct cctcttgatc
     6001 ggttccgcca ccgccgaccg gagccgcgac cttctcgccc cagtcaccgg atgagctggc
     6061 gctggtggag cgggactgcc gctcgacccg tgccgtggcc caccggaggc tgggaccgac
     6121 ctcgtcggcc tcgatctcga tggcgttgcg cttctcgccg tcctggttct cccacgagcg
     6181 gtagttcagg cgcccggcca cgaccacgcg cgatccgcgg gttagcgact cggccacgtt
     6241 ctcgcccagg ctgcgccagc agttgacgct gaagtacgac gcttccgcgt ccttccagcc
     6301 accggcaccg tcggggatac ggcggttcac ggcgatccgg aacttgcaca ccgccgcacc
     6361 gttgggcgtg tagcgcagct cgggatcgtc ggtcaggttg cccaccaggg tcacctggtt
     6421 gtcactcgcc atggttgcct caccacctgt tctgggtcca cgtcctcacg ccgtctcggc
     6481 ggggacgggg gaagaagctg cttccttctg gtcgcgtcgg ttctgacgcg agttcgctcg
     6541 ctgctcgggc aggaccagga ccttgaaccg gatgacctca tcggccaggt tcaggacacg
     6601 atccaactcg gcctgggtct cgggctgcgc ggtgaacttg gccaccacgt aatagccctc
     6661 ggtgaggtgc ttcagctcgt aggcgaagcg ccggcgaccc cagcgatcga tgttggagac
     6721 ctcaccaccg ctggcggaaa tcaccttggt gatgcggtcc atcgccgtgg acacgacgga
     6781 ttcgtcggcc tcggcgggca ggatgagcat gatctcgtac tccctcaatc ttcacctcct
     6841 ctggtctgga cggccccctc acatgagagg gagcagaaag gaaatgaggg tcgcccagca
     6901 gtgtacggga acgccgcgaa cgctagcagc gggctctgac accgcctggc cctccggctg
     6961 agccggatcg ccgggagggc cgccggccgg gcggaaggac aggtacccgg ccggcggcgc
     7021 gac
//
LOCUS       DATDBZ010000088         3020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_848419, whole genome shotgun sequence.
ACCESSION   DATDBZ010000088 DATDBZ010000000
VERSION     DATDBZ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848419"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VIX62_02100"
     CDS             <1..417
                     /locus_tag="VIX62_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AhpC/TSA family protein"
                     /protein_id="HEY5859060.1"
                     /translation="ARRLTTELDEYRAAGLSVFIVGQGEPARASAYAHEHGITVPLLL
                     DPTADVYRAYGVGHWQPEQLLFDAPAEYLAHPRALGQSLLEARRAEGRPMVDDPWRAS
                     GEFIIGQNGTVRLGFSYQYCEDFPDPRVLTAAARLA"
     gene            complement(517..999)
                     /locus_tag="VIX62_02105"
     CDS             complement(517..999)
                     /locus_tag="VIX62_02105"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY5859061.1"
                     /translation="MSNARRGRPSVPPRWFVRLAWKVHRGIYRISRGRVGLWRPKPRG
                     WGWGTLHLTTTGRRTGQERKVILGYVEDGPNLVTMAMNGWADPEPAWWLNLQARPDAM
                     VVVRDGPRLVHARAAEGDERSRLWAKWREIDTGLDDLAALRSSETTVVILEPRSGQNL
                     "
     gene            1138..1680
                     /locus_tag="VIX62_02110"
     CDS             1138..1680
                     /locus_tag="VIX62_02110"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY5859062.1"
                     /translation="MKLVTRAMALYENLPLPAGSALGVVAVLVLDRARPAPLPGSRAF
                     QRAVGAAAFAAGFALNLWALVERRRRTSGAFQLEQPESIVTTGPYAFSRHPMYAGWWL
                     IHVGVGLLRGTTWMAATVPVAVLTEHFGGSVVEERELRRQFGADYARYAERVPRYAGW
                     PRVRPTGAGNDRDRGVASLP"
     gene            complement(1750..>3020)
                     /locus_tag="VIX62_02115"
     CDS             complement(1750..>3020)
                     /locus_tag="VIX62_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859063.1"
                     /translation="FFLYATAATLVFNVIFFPPSDNPLDNIIKAFLTYAVGFIARPLG
                     GIVFGHFGDKYGRKKLLQLAIILVGVSTFLMGCLPTFDQVGYAAPIMLIILRFFQGFA
                     VGGEWGGAVLLVAEHSPDKQRGFWASWPQAAVPAGNLLATVVLLTLSRTLSEEQFLAW
                     GWRVGFWLSVVIVAVGYYIRTKVTDAPIFIEAQAEVEAAKKTNYGVIEVLKRYPRGVF
                     TAMGLRFGENIMYYLVVTFSITYMSAHLGMDTSTILGLVLFAHVVHFCVIPVVGRFSD
                     KVGRKPVYAVGAVLAAMWGFVAFPLFDTQNDWLIVGVLILGLVIHALMYASQPAIMAE
                     MFPTRMRYSGVSLGYQVTSIVAGSLAPIIATMLLSTYGSWVPVAIYLALAAGVTLVAV
                     IALRETVGVSLRAIDDADRAKVASEQKVDA"
BASE COUNT          503 a         1010 c         1043 g          464 t
ORIGIN      
        1 gcccgacgac tcaccacgga actcgacgaa taccgcgcgg cgggcctttc ggtgttcatc
       61 gtggggcagg gcgagccggc gcgggcctca gcctatgccc atgaacacgg catcaccgtg
      121 ccgctgctcc tcgatccgac cgccgatgtc tatcgggcat acggcgtcgg tcattggcaa
      181 cccgagcaac tgctgttcga tgcgccggcc gagtacctcg cgcacccgcg cgcgctcggg
      241 cagagcttgc tcgaggctcg acgtgcggag ggtcggccga tggtcgacga cccgtggcgg
      301 gcctcgggcg aattcatcat cggccagaac ggcaccgtcc gactcggttt ctcgtatcag
      361 tattgcgagg acttccctga tccacgggtg ctcaccgccg ccgcgcggct ggcatagatt
      421 catcgccggc gatcccgttg gttaccgatg tgcgccggct cggcggccgg ctgatgcggc
      481 ccccgaagcc gggctgattc ggcccccgac gccgggtcag aggttctgac cggaccgtgg
      541 ctccagaatg acgaccgtgg tctcggacga ccgaagcgcc gcaaggtcgt ccagcccggt
      601 gtcgatctcg cgccacttgg cccacagacg tgagcgctcg tccccctccg cggcgcgggc
      661 atgcaccagg cgcgggccgt cgcgaacgac caccatcgca tcgggtcggg cctggagatt
      721 gagccaccac gccggttctg ggtctgccca accgttcatc gccatcgtga cgaggttcgg
      781 accgtcttcg acgtagccga ggatcacctt tcgctcctgg ccggtgcgcc gcccagtggt
      841 cgtcaagtgg agcgtgcccc agccccagcc tcgtggtttc gggcgccaca acccgactcg
      901 accacggctg attcggtaga tgccccggtg gaccttccat gcgaggcgca cgaaccagcg
      961 aggtggaacg ctcggtcgtc cccgtcgtgc gttggacacg ggtggagtct agtcgccggg
     1021 tccaacgcga gtggcttcac aaggcgttgt gcgcaacccg cgcgagggct cgtgccggcc
     1081 cgccaatggg tgattcgccc gatgctgcgg ccgaggtcag gccgtagcgt cggaagcatg
     1141 aagctcgtca cgcgtgcaat ggccctgtat gagaacctcc cgctgcccgc agggtcggcg
     1201 ctgggcgtcg tcgcggtgct cgtgctcgac cgtgctcgtc ccgcgccgct ccccggctct
     1261 cgagcgtttc aacgtgcggt gggcgccgcc gcgttcgccg cagggttcgc gctgaacctg
     1321 tgggcgctcg tcgaacgccg ccgtcgcacc tctggggcgt tccagctcga acagcccgaa
     1381 tcgatcgtca ccaccggacc gtacgccttc agccggcacc ccatgtacgc cggatggtgg
     1441 ctcatccacg tcggtgtcgg cctgctgcgt ggaaccacct ggatggcggc gacggtgcct
     1501 gtagcggtcc tcaccgagca cttcggcggc tcggtcgtcg aggagcgcga gctccgacgt
     1561 cagttcggcg ccgactatgc gcggtacgcc gagcgggtgc cgcgctacgc gggctggccg
     1621 agggtgcggc cgactggcgc cgggaatgac cgtgaccgag gagtggcgtc actgccctga
     1681 acgcacggat gccgcgaccc gaccggctcc gaagaaccgg ccgcccgcgg catccgtcag
     1741 tgaatccgac tacgcgtcga ccttctgctc ggaggcgacc ttcgcgcggt cggcgtcgtc
     1801 gatcgcccgc agcgaaacgc cgacggtctc gcgcaacgcg atcaccgcga cgagcgtgac
     1861 gcccgcggcg agtgccaggt agatcgccac cggcacccag ctgccgtacg tcgacagcag
     1921 catcgtcgcg atgatcggag cgagcgagcc cgcgacgatc gacgtgacct ggtagccgag
     1981 cgaaacgccc gagtagcgca tgcgcgtcgg gaacatctcg gccatgatcg ccggctggct
     2041 ggcgtacatg agggcgtgga tgacgaggcc gaggatcagt acgccgacga tgagccagtc
     2101 gttctgcgtg tcgaacagcg ggaaggccac aaagccccac atggcagcga gtacggcgcc
     2161 gaccgcgtag acgggcttgc ggccgacctt gtcgctgaac cgcccgacga ccgggatgac
     2221 gcagaagtgc acgacgtgcg cgaagagcac caggcccagg atcgtcgagg tgtccatgcc
     2281 gaggtgcgcc gacatgtacg tgatcgagaa cgtcacgacg aggtagtaca tgatgttctc
     2341 gccgaaccgc aggcccatcg cggtgaagac accgcggggg tagcgcttga gcacctcgat
     2401 gacaccgtag ttggtcttct tcgcggcctc gacctcggcc tgcgcctcga tgaagatcgg
     2461 cgcgtcggtg accttcgtgc ggatgtagta gccgacggcg acgatcacga ccgagagcca
     2521 gaagcccact cgccaacccc acgcgaggaa ctgctcctcg gagagcgtgc gcgagagcgt
     2581 gaggagcacg accgtggcga gcaggttgcc ggcaggcacc gcggcctgcg gccagctcgc
     2641 ccagaacccg cgctgcttgt cgggcgagtg ctcggcgacg agcaacaccg cgccgcccca
     2701 ttcgccaccg accgcgaagc cctggaagaa ccgcaggatg atgagcatga tgggcgcggc
     2761 gtagccgacc tggtcgaacg tgggcaggca gcccatgagg aacgtcgaga cgccgaccag
     2821 gatgatggcg agctgcagca gcttcttgcg gccgtacttg tcaccgaagt ggccgaagac
     2881 gataccgccg agcgggcgcg cgatgaagcc gacggcgtag gtgaggaagg ccttgatgat
     2941 gttgtcgagc gggttgtcgc tcggcgggaa gaagatgacg ttgaagacca gggtcgccgc
     3001 ggtggcgtag aggaagaact
//
LOCUS       DATDBZ010000089         3555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1463022, whole genome shotgun sequence.
ACCESSION   DATDBZ010000089 DATDBZ010000000
VERSION     DATDBZ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463022"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..1194
                     /locus_tag="VIX62_02120"
     CDS             355..1194
                     /locus_tag="VIX62_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY5859064.1"
                     /translation="MSILQAIILGITQGLTEYAPVSSSGHLILVPWAFGWTIVNDPAL
                     NKTFDVALHMGTLTGALIYFRHDVVRYVRAWIESLRARAIENADQRLAWALVVGTIPG
                     VIIGAAFEGFISDTLGQPWLIGVMLVVFGVALWWVDKVAASDRGLDSITPKTGLGLGF
                     AQAMALQPGVSRSGVTMTAGRWIGLDRESAARFSFLLALPITAGAGLYASIDVVQGGF
                     QGYAAQFFWGFVASGISGFLVIWGLLKYLRTHSFAAFMWYRFAVAGIIFVLIASGARS
                     ATI"
     gene            1219..1635
                     /locus_tag="VIX62_02125"
     CDS             1219..1635
                     /locus_tag="VIX62_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5859065.1"
                     /translation="MDRRSVQAWLDRYIDTWRSGDAAEIAALFSRDAEYRYHPSDDPI
                     RGPEQIAASWLETPDEPGTWDAWYRPFVVEGERAVATGVSTYFHPDGSADRVYDNVFV
                     MTFDTQGRCSEFTEWFVQRPSDRLDAAVPEPLVPEG"
     gene            complement(1642..2691)
                     /locus_tag="VIX62_02130"
     CDS             complement(1642..2691)
                     /locus_tag="VIX62_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEY5859066.1"
                     /translation="MSDDFTNVELAQATRNHGMPLEALRYDVTPAGLHYLLIHYDIPP
                     VDPNFTLTVDGAVEGAGSWTLEELRAMRSVTVPVTLECAGNGRTLYERRPESQPWIRE
                     AVGTAAWTGVPVAELLERAGLRDDAVDVVFTGADRGVEGGEEQAYARSLPPAELQRDE
                     VLLAYEMNGAPLLPQHGAPLRLMVPGWYGMTAVKWLTSITAVTEPFEGYQQRVGYRMR
                     TSDDDPGTPVTVIAPRALMVPPGIPAFPSRARSLQAGPTTLDGRAWSGHAPIERVEVS
                     TDDGRSWTDATLEPEIGRFAWREWHHPWDAHAGSHVVCCRATDAEGNTQPDEPEWNAG
                     GYAHNGIQRVEVQVE"
     gene            complement(2753..>3555)
                     /locus_tag="VIX62_02135"
     CDS             complement(2753..>3555)
                     /locus_tag="VIX62_02135"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY5859067.1"
                     /translation="GDETQTGFSYRIARALSDVGPDDIADEAITRAVDMLGAVKPPTA
                     KVPVILDQFAAMSFLGVLAGALNAESVLKGRSLFASMVGQQVGSDLFTLVDDGTILQG
                     PGACPFDDEGVPSGRTELFTGGTLNGFLHDTYTAARTGDGQASTGNAKRGSYKGAPGV
                     GTSNFYLDAGETSFDELLQRADGGVLIQDVSGVHSGANPISGEFSVGATGLRIAGGAL
                     GEPLREMTIASTLPEMLAGITGVGDDLRFFSSVGTPSILIGEMTLAGI"
BASE COUNT          508 a         1254 c         1215 g          578 t
ORIGIN      
        1 gccggccttc ccggcgtgtg acggtgatct cgctgctcgt ccgtcggagc acaagccggg
       61 gcccggcggc cccggcgttg gcaccgggcc cagtcagggc catcacggga atcgcgagcg
      121 ccgcggccaa caggccgctc agggcgcggc gcgtcgtggt catgatttcc tcccacgtcg
      181 tcgtgcgttg gaccgaggaa gccccatcgg tcatctccta gacgcgcgag cccgcgtccc
      241 ggttgcgaag ctaggcgcag ttgccacccc gacgcaaccc aacgttcgct cggttcccgt
      301 gggttcgggc cgctagcatg tgcgggcctc ccgaccgaaa gggcacgacg ctccgtgagc
      361 atcctgcaag cgatcatcct gggcatcacc cagggactga ccgaatacgc gccggtctcc
      421 agctccgggc acctcatcct ggtgccgtgg gcgttcgggt ggacgatcgt gaacgacccg
      481 gcactgaaca agacgttcga cgtggcgctg cacatgggca cgctcaccgg agcgctcatc
      541 tacttccggc acgacgtcgt ccgctacgtg cgcgcctgga tcgagtcgct ccgcgcccgc
      601 gccatcgaga acgcggacca gcgcctcgcg tgggccctgg tggtgggcac catccccggg
      661 gtgatcatcg gcgcggcctt cgaggggttc atctccgaca ccctgggcca gccatggttg
      721 atcggcgtga tgctcgtggt gttcggcgtg gccctgtggt gggtcgacaa ggttgccgcc
      781 agcgaccgcg gcctcgattc gatcaccccc aagacggggc tcgggctggg gttcgcccag
      841 gcgatggccc tgcagcccgg ggtctcacga tcgggcgtca ccatgaccgc gggccgatgg
      901 atcgggctcg atcgcgaatc cgcggcgagg ttctcgttcc tgctggccct gccgatcacg
      961 gcgggcgcgg gcctctacgc gtccatcgat gtggtgcagg gtgggttcca gggctacgcc
     1021 gcgcagttct tctgggggtt cgtggcgtcg gggatcagcg gcttcctggt gatctgggga
     1081 ctgctgaagt acctgcgcac ccacagcttc gcggccttca tgtggtatcg gttcgcggtg
     1141 gcggggatca tcttcgtgct gatcgcgagc ggagcgcgga gcgcaacgat ctgacccgag
     1201 gcggacaagg aggcgaccat ggatcgacgc agcgtgcagg cttggctcga ccgatacatc
     1261 gacacctggc ggtccgggga cgcggccgag atcgcggccc tcttcagccg cgacgcggag
     1321 tatcgctacc acccgtccga cgatcccatc cggggcccgg agcagatcgc cgcttcgtgg
     1381 ctcgagaccc ccgacgaacc cggcacgtgg gatgcgtggt accgaccgtt cgtggtggag
     1441 ggcgagcgcg cggtcgccac cggcgtcagc acctacttcc atcccgacgg gagcgccgac
     1501 cgcgtctacg acaacgtgtt cgtcatgacc ttcgacacgc agggccggtg cagcgagttc
     1561 accgagtggt tcgtgcaacg accgtcggac cgactggatg ccgccgttcc cgagccgctg
     1621 gtcccggaag gctgactcgc ctcactccac ctggacctca acgcgctgga tcccgttgtg
     1681 cgcgtagccc ccggcgttcc actcgggttc gtccggttgg gtgttgccct cagcgtcggt
     1741 ggcgcggcag cacacgacgt gcgaacctgc gtgagcgtcc cacgggtggt gccactctcg
     1801 ccatgcgaac cgtccgatct cgggctccag ggtggcatcg gtccacgacc tgccgtcgtc
     1861 ggtgctgacc tcgacgcgtt cgatcggcgc atgccccgac caggcacgcc cgtccaacgt
     1921 ggtcggaccc gcctgcaacg accgggcccg cgaggggaac gccgggatgc ccggcggcac
     1981 catcagcgcc cgaggagcga tcacggtgac gggcgtgccg ggatcgtcgt ccgacgttcg
     2041 catgcggtag cccacccgct gctgatagcc ctcgaacggt tcggtgaccg cggtgatcga
     2101 cgtgagccac ttgaccgcgg tcatcccgta ccacccgggc accatcaggc ggagcggggc
     2161 cccgtgttgc ggaagcagcg gagctccgtt catctcgtag gccagcagca cctcgtcccg
     2221 ctggagttcc gcaggcggca ggctccgggc gtaggcctgc tcctcgccgc cttccacacc
     2281 acgatcagct ccggtgaaga ccacatcgac cgcgtcgtcg cggaggccgg cccgctccaa
     2341 cagctcggcc accggcaccc ccgtccacgc tgcggtgccc accgcttcac ggatccacgg
     2401 ctgggactcg ggacgtcgtt cgtagagggt ccggccgttg ccggcgcact cgagcgtcac
     2461 cggcaccgtg accgatcgca tggcgcgtag ctcctcgagc gtccacgacc ccgctccctc
     2521 gacggcgcca tccaccgtca gggtgaagtt cgggtccacg ggcgggatgt cgtagtggat
     2581 cagcaggtaa tgcagccccg ccggggtcac gtcgtagcgc agggcctcaa gcggcatccc
     2641 atggttccgg gtggcctgcg ccagttcgac gttcgtgaag tcgtcgctca tcggtgtcgt
     2701 cgcgccggat ggcgcgctcc cgagtctacg cccggggtcg tcggcgctcg acctagatgc
     2761 cggccagggt catctcgccg atcaggatcg acggggtgcc gacgctcgag aagaagcgga
     2821 ggtcgtcgcc caccccggtg atgccggcca gcatctccgg cagcgtcgag gcgatcgtca
     2881 tctcgcgcag cggctcgccc agggctcccc cggcgatgcg cagccccgtg gcgccgacgc
     2941 tgaactcgcc gctgatcggg ttcgctcccg agtgcacccc gctgacgtcc tggatcagga
     3001 cgccgccgtc ggcccgctgc agcagttcgt cgaacgaggt ctcgcccgca tccaggtaga
     3061 agttggaggt gcccacgccc ggcgcgccct tgtacgagcc gcgcttcgcg ttgccggtcg
     3121 aggcctgccc gtcaccggtt cgagccgccg tgtaggtgtc gtgcaggaac ccgttcagcg
     3181 ttcccccggt gaacagttcg gtgcggccgc tgggcacgcc ctcgtcgtcg aaggggcagg
     3241 cacccggtcc ctgcaggatc gtgccgtcat cgaccagggt gaacaggtcc gagcctacct
     3301 gttggcccac catcgaggcg aacagcgacc gccccttcag caccgattcg gcgttgagcg
     3361 cgcccgcgag cacgcccagg aacgacatcg ccgcgaactg atcgaggatc accggcacct
     3421 tggcggtggg gggcttcacc gcgcccagca tgtcgaccgc gcgcgtgatg gcttcgtcgg
     3481 cgatgtcgtc gggcccgacg tcggagagcg cccgggcgat gcgataggag aagccggtct
     3541 gggtctcgtc gccct
//
LOCUS       DATDBZ010000090         4463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2077910, whole genome shotgun sequence.
ACCESSION   DATDBZ010000090 DATDBZ010000000
VERSION     DATDBZ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077910"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VIX62_02140"
     CDS             <1..654
                     /locus_tag="VIX62_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859068.1"
                     /translation="ISMVLALRSGNEQIIARLAPFGRDEGWPLLTNVVTQIAAAGGLI
                     AFAVALSWIVGREFADGTIAALFAQPISRGTIASAKLLVFVIWGSATGAILVLVIAMV
                     GIGLGYGLPDGIALASLGRIFLLTVLSTFIAIPVGWVSTLGRGLLAGIAAGVGIVVIA
                     QFGVVSGAGGWMPFAAPAMWAISPETVTLVQLACVLIVPTIFWPATVLAWRRLQLDR"
     gene            850..1521
                     /locus_tag="VIX62_02145"
     CDS             850..1521
                     /locus_tag="VIX62_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEY5859069.1"
                     /translation="MRFRQHGLSIAFGVIFALALIGQSFAGLQVFNEEQLEHGLDPVT
                     YFEFVTSSEFVVDVAENWQSEYLQFSLFVFATIWLVQKGSPESKKPGDEGLGTDEDQL
                     VHEHAKPDSPKWAKANGWRHTLYSNSLLLVMGMIFVLSWLIQSLSGVVVYNAEQAEHG
                     EQAITWGEYLFAADFWNRTLQNWQSEFLAVGSMIALSIFLRQRGSSESKPVGAPHEVT
                     SVEAE"
     gene            complement(1578..1865)
                     /locus_tag="VIX62_02150"
     CDS             complement(1578..1865)
                     /locus_tag="VIX62_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010155360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY5859070.1"
                     /translation="MTNATSSASVHDADAQRKIVNRLRRAQGQLAAVITAVENDAHCR
                     EVAQQLAAVSKALDRAGFLVIATALKDCLANPDAEDLPQPEELEKLFLSLA"
     gene            2730..3941
                     /locus_tag="VIX62_02155"
     CDS             2730..3941
                     /locus_tag="VIX62_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEY5859071.1"
                     /translation="MRSLTFNGSLPGPTLRVRPGDRIALTFRSELDAPTNLHTHGLVV
                     SPEGNSDNVFLLIEPGATFDYAYELGDDHPPGVFWYHPHHHGNSAEQLFAGLYGAIIV
                     DDEEPVAAARERLLMISDIAFDGADVHVASAGERRLGREGDLVMVNGQVGATLIARPG
                     ERERWRIINACSSRYLRLRLDGQRMQLLGVDAGRYAEPLDVDEIVLAPGNRADVMMTA
                     VRGTSVLQTLPFDRGSMGMFGSPSSATSGADLLTLAVAGEPATAAPLTVPLPTTRDLR
                     AEQMTGARTITLAMGPGGGGMGFTIDGREFDHDRIDTEVSHGSLEEWTIVNQSPMDHP
                     FHLHAWPMQLIEVAGQPVEGIDWRDVVPVPARSRAIVRIAFEGITGLTVYHCHILDHE
                     DLGMMGTIRAG"
     gene            4020..>4463
                     /locus_tag="VIX62_02160"
     CDS             4020..>4463
                     /locus_tag="VIX62_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859072.1"
                     /translation="MTQKRYLIIGGVAGGMSAATRLRRLDENARITVLERGGHVSFAN
                     CGLPYYIGGVIEQRDSLLLQTPASLGSRFNLDVRVRTEAVSIDRDAKTVRIRDLTTGE
                     ETDEAYHALVLSPGATPVRLPLPGGERMLTLRDIDDVDAAMAELTV"
BASE COUNT          704 a         1480 c         1510 g          769 t
ORIGIN      
        1 atcagcatgg tgctcgcgct gcgatcagga aacgagcaga tcatcgcccg gctcgcaccg
       61 ttcggtcggg acgaaggttg gccgcttctg acgaacgtgg tcacccagat cgccgcggcg
      121 ggtgggctca tcgccttcgc cgtggcgctc agctggatcg tcggtcgcga attcgcggac
      181 ggcacgatcg cagccctgtt cgctcagccg atcagccgcg gaacgatcgc atcggcgaag
      241 cttctcgtgt tcgtcatctg gggttcggcg accggcgcca tcctggtgct cgtgattgca
      301 atggtgggca tcggcctcgg gtacggtctt ccggacggaa tcgcgctcgc ctccttgggc
      361 cggatcttcc tcctcacagt gctctctacg ttcatcgcga ttcctgtcgg ttgggtatcc
      421 acgctcgggc gcggtcttct cgccgggatc gcggccggtg tcggcatcgt cgtgatagcg
      481 cagttcggcg tcgtctcggg ggcgggcggc tggatgccct tcgcggcgcc cgcgatgtgg
      541 gcgatctcac cggagaccgt gaccctggtc cagctcgcgt gcgtcctgat cgttcccacg
      601 atcttctggc cggccactgt actcgcctgg cgccgactgc agctcgatcg atgagatctt
      661 gtcgcgcccg cgccatccgt gccattgcga catccgtcga ttcgcgccgt cccggcagtg
      721 cgcgggtcga gcgccgggtc cgggcgctac ggtcgggcgt atgagagcgg tgttcacgtc
      781 atggctcgtg gtgatcgggg tcggcttggc gtacatgctg acgatctcgt tgctgggcag
      841 gtgaggtcgt tgcgtttccg acagcacggc ctgagcatcg cgttcggcgt catcttcgcg
      901 ctggcgctga tcggccagtc cttcgcgggc ctgcaggtct tcaatgagga gcagctcgaa
      961 cacgggctcg accctgtgac ctacttcgag ttcgtgacct cgtccgagtt cgtcgtggat
     1021 gtggccgaga actggcagtc cgagtatctg cagttctccc tgttcgtctt cgcgacgatc
     1081 tggctcgtgc agaagggctc tcccgagtcg aagaagcccg gcgacgaagg gctcggcacc
     1141 gacgaggacc agctcgtgca cgagcatgcc aagcccgatt cgcccaagtg ggcgaaggcg
     1201 aacgggtggc gccacacgct ctactcgaac tcgctgcttc tcgtgatggg catgatcttc
     1261 gtgctgtcgt ggctgatcca gtcgctgtcc ggcgtcgtgg tgtacaacgc cgagcaggcc
     1321 gagcacggcg agcaggcgat cacctggggg gagtacctgt tcgccgcgga cttctggaac
     1381 cgcaccctgc agaattggca gtccgagttt ctcgcggtcg gcagcatgat cgcgctgtcg
     1441 atcttcctcc gccagcgcgg ctcgtcggag tcgaagccag tcggcgctcc gcacgaggtg
     1501 acgagcgttg aggcggagtg aacaccggtt gagcagtgcc ccacgcgagt cggagcacac
     1561 ctcgggtcgc cgcccagtca ggcaagtgag aggaagagct tctccagttc ctcgggctga
     1621 gggaggtcct cggcatcggg gttggcgagg cagtccttca gcgcggtggc gatcacgagg
     1681 aagccggccc gatcgagggc cttcgaaact gccgcgagtt gttgcgccac ctcgcggcag
     1741 tgggcatcgt tctccaccgc cgtgatgacc gctgccagtt gtccctgggc tcggcgcaga
     1801 cgattcacga tcttgcgctg agcgtcagcg tcgtggactg aagccgagga ggtcgcattc
     1861 gtcatttggg aagcccttca tgggtggatt cgtcatcgag catactcccc acagtatagt
     1921 tggttataca cccggggtat cacccagaaa ccgcggggga gtcttcgccc gcctgttcgg
     1981 ccgataggag acggcatcct ggtcttctcc cagccgggtg cgttgcgagc gccgcaactg
     2041 gaacagctcg tcgagggcgc gcgggcactg gacatggaca gcgtgcgacg ccagatcacg
     2101 gaccgagatg ccagcacggc cccggcttga ccagaacctg ctgacggagc acatctacga
     2161 cgaggatctc gctcaggcga gctaccgggc ggcttcgtgt tcgtcggtga cgtcggtcga
     2221 cccgacctgc tcgacgaggc gaccggcgcg atcgacaccc taatgcagcg gcaactcgac
     2281 gctgcaggcc gcgacgatca agcacgaaaa agacgagatg gcggcgatct cgaggtcact
     2341 acgtgggacc gggagcgcga ggcgaaggtc ccgaacgatc ctcggaggcg accgtagcct
     2401 ccaagtggag gtggcgtcat gttcgatgag aatccgacgc cgagcgcggg acgtgacagt
     2461 gggggaatca cccgccgcaa cgcgctcctc ctgggcggcg ttggcgcggc cgctctcgct
     2521 ggggcggtct ctggctttgg agccaggggc gcaaagaacc cgaagccccg gcgggggcga
     2581 cccccgaggc gagcccctcg ctcaacccgt cgccacagct ggaggacatc ggcaccctca
     2641 ccgagcccga agtcctccag agttccgacg gcctcctcga gctcaccatc gccgccgcgc
     2701 tgaccgacgt cgtggtagca ggtcggcagg tgcggtcgct gacgttcaac ggcagcctcc
     2761 cgggtcccac actgcgggtc cggcccggcg atcggatcgc cctgacgttc cgcagcgagc
     2821 tggatgcgcc gacgaacctg cacacccacg gcctcgtcgt ctcgcccgaa ggcaacagcg
     2881 acaacgtgtt cctcctgatc gagcctggcg ctacgttcga ctacgcctac gagctcggcg
     2941 acgaccaccc gcccggggtg ttctggtatc acccgcatca ccacggcaac tccgcagagc
     3001 agctcttcgc cggcctgtac ggcgcgatca tcgtcgacga tgaggagcct gtcgcggccg
     3061 cccgggagcg gctgctcatg atctccgaca tcgcgttcga cggtgccgac gtgcacgtgg
     3121 catccgccgg cgagcgcagg ctggggcgag agggcgacct ggtcatggtc aacggccagg
     3181 tgggcgcgac gctgatcgcg aggccggggg agcgggagcg ctggcgcatc atcaacgcgt
     3241 gcagctcgcg ctacctgcgg cttcgactcg acggccagcg gatgcagctg ctcggtgtcg
     3301 atgccggccg ctacgcggag cccctggacg tcgacgagat cgtgctcgca cccggcaatc
     3361 gcgcggacgt catgatgaca gccgtgcggg ggacgagcgt gcttcagaca ctgccgttcg
     3421 atcgcggcag catggggatg ttcgggagtc cgtcgagtgc gacgtccggc gcagacctcc
     3481 tcaccctggc ggtggcgggt gagccggcca ccgctgctcc gctcacggtt ccgctcccga
     3541 cgacgaggga cctccgcgcc gagcagatga ccggagcgcg gaccatcacc ctcgcgatgg
     3601 gtccgggcgg aggcggcatg ggtttcacga tcgacggccg cgagttcgac cacgaccgca
     3661 tcgacaccga ggtctcgcac gggtcgctcg aggagtggac catcgtgaac cagagcccga
     3721 tggaccatcc gttccatctg catgcctggc ccatgcagct catcgaggta gccgggcagc
     3781 ccgtcgaggg catcgactgg cgcgatgtgg tgccggtgcc ggcccgcagc cgcgcgattg
     3841 tgcggatcgc attcgaggga attaccggcc tgacggtgta tcactgccac atcctcgacc
     3901 acgaggatct cggcatgatg ggcacgatcc gcgcgggttg acgcgtcgcc cacgagacat
     3961 ggtgtgaata ccccgggggt attttgacaa taccccgggg gtatccgtac gattgctgca
     4021 tgacccagaa gcgctacctc atcatcggcg gagtcgccgg cggcatgtcc gcggccaccc
     4081 gcctgcgccg gctcgacgag aacgcccgga tcacggtgct cgagcgcggt ggccatgtct
     4141 cgttcgcgaa ctgcggactg ccctactaca tcggcggcgt catcgagcag cgcgacagcc
     4201 tgctcctgca gaccccggcg tctctcggca gccggttcaa cctcgacgtg cgcgtgcgca
     4261 cggaggccgt ctcgatcgac cgcgacgcca agacggttcg catccgcgac ctcacgacgg
     4321 gggaggagac cgacgaggcc taccacgcgc tcgtgctctc gcccggggcg acgcccgtgc
     4381 gcctcccgct tcccggcggc gagcgcatgc tcacgctccg cgacatcgac gacgtcgacg
     4441 ccgcgatggc cgagctcacc gtc
//
LOCUS       DATDBZ010000091         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2486961, whole genome shotgun sequence.
ACCESSION   DATDBZ010000091 DATDBZ010000000
VERSION     DATDBZ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486961"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..290)
                     /locus_tag="VIX62_02165"
     CDS             complement(15..290)
                     /locus_tag="VIX62_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859073.1"
                     /translation="MPETISDARKHLTGHVARFRAEGLDAEPVVFGDHRQPEAALLPF
                     ETFQLLLDVAEDIAIAQRIRERESSDSGNRTSLAEVAAEFGIDLDGL"
     gene            complement(415..990)
                     /locus_tag="VIX62_02170"
     CDS             complement(415..990)
                     /locus_tag="VIX62_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEY5859074.1"
                     /translation="MKGTLAVTGDPKADRLLNTDGTALLIGMLLDQQVPMEWAFTGPA
                     TLKARLGHLDAKKIAAMDVDAFVDVCREKPAIHRFPASMGKRIHELCVALTERYKGKA
                     ENVWKGVATGDELYQRLRALPGYGDEKAKIFLAILAKRVGVQPEGWQVAAGAFGDNTP
                     RSVADIYSPASLAVVREWKKAQKAAKKTKQE"
     gene            complement(1172..1729)
                     /locus_tag="VIX62_02175"
     CDS             complement(1172..1729)
                     /locus_tag="VIX62_02175"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY5859075.1"
                     /translation="MVALDLPRHRWTRTEYALLDPDVFARCELIEGEIIDVSPMSRAH
                     ALVTDQIRDLMSRLVDPMVMCAGSQTPIIIGEYSEPEPDVWVAHVPRRNLAEGKPLPE
                     ELALVIEVAESSLRVDRRLKLPLYASAGIPVMWLVDLAAERIEIHGEPDAAERRYATV
                     QLHEEGSSVPLPWGGEVAFNELLLR"
     gene            complement(1792..>2282)
                     /locus_tag="VIX62_02180"
     CDS             complement(1792..>2282)
                     /locus_tag="VIX62_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY5859076.1"
                     /translation="VLFSGSAQGAAREAAAQLAEHYDVGVELWSATSYKVLREQALDT
                     ERWNRLHPGQPARTPLVAELLTEAPGPIVAVTDFMKIVPEQVARFLPGRVFTPLGTDG
                     MGRSDTRESLRRLFEVDAAHVIVAVLAGLAAHGDVKPETVQDAIQRYGIDPEALPATH
                     LP"
BASE COUNT          370 a          764 c          757 g          391 t
ORIGIN      
        1 tggatccgtt gcgctcacag cccgtcgagg tcgataccga actccgcggc gacctccgcc
       61 agcgaggttc ggttacccga atcagatgat tcacgctcac ggatgcgctg ggcgatggcg
      121 atgtcctcgg cgacgtccaa cagcagctga aaggtctcga acggcagcag ggctgcctcc
      181 ggctggcgat ggtcgccgaa caccacgggt tccgcatcca ggccttccgc gcggaatctg
      241 gccacatgcc cggtcaggtg tttccgagca tcgctgatcg tctcaggcat cactctctcc
      301 tgtacaggtt catgtacaga cgatggtacc cgcagacccg tcaacgtcaa ccatcgggct
      361 gcctatcgtg tgtcccgaac gccaacggct ggcctggatc tcatgacgtc gagctcactc
      421 ctgcttggtc ttcttcgccg ctttctgcgc cttcttccat tcgcgcacga cggcgagcga
      481 cgcgggtgag tagatgtcgg cgaccgatct cggcgtgttg tcaccgaagg cgccggctgc
      541 cacctgccat ccttctggct gcacgccgac ccgcttcgcc agtatcgcca ggaagatctt
      601 cgccttctcg tcgccatatc ccgggagggc tcgcagtcgc tggtagagct cgtcgccggt
      661 ggcgacaccc ttccagacgt tctctgcctt ccccttgtac cgctcggtga gcgccacgca
      721 gagctcgtgg atccgcttgc ccatcgacgc cgggaagcgg tggatcgccg gcttctcgcg
      781 gcacacgtcg acgaacgcgt cgacgtccat cgccgcgatc ttctttgcgt cgagatggcc
      841 gagccgtgcc ttgagcgtgg ccggaccggt gaacgcccac tccatcggga cctgctgatc
      901 gagcagcatt ccgatcaaca gtgcagtgcc atcggtgttc aacagacggt cggccttcgg
      961 atcgccggtg acggcgaggg tgcccttcat gcgcgagagc ctatgacggt cgccgcgagc
     1021 acgacggctc ggtcgatgaa tcaccaacca tctgccgcca caaagtcagg cgtcgcccag
     1081 gacacctcct cgcgccacaa aggcgtcaag ttgccgggca cagaccgaga cgctgttcgc
     1141 gagcggatgg attgtgcctg cgtcgcgagt gtcagcgcag cagcagctcg ttgaaggcga
     1201 cttcgccgcc ccacggcagg ggcacggacg aaccctcctc gtgcagctgc acggtggcgt
     1261 agcgccgctc ggccgcgtcc ggctcaccat ggatctcgat ccgctccgcg gccaggtcga
     1321 cgagccacat gacggggatg ccggcagatg cgtacaacgg cagtttcagg cgccgatcga
     1381 cacgcagcga cgactcggcc acctcgatca ccagcgccag ctcctccggg aggggcttgc
     1441 cctcggccag gttgcggcgg ggcacgtgcg cgacccagac gtcgggttcc ggctcgctgt
     1501 actcaccgat gatgatcggc gtctgtgatc cggcgcacat caccatcgga tcgaccaatc
     1561 gtgacatcag gtcgcgaatc tggtcggtga cgagcgcgtg ggcgcgcgac atgggtgaca
     1621 cgtcgatgat ctccccctcg atgagctcgc accgcgcgaa cacgtccgga tcgagcagcg
     1681 cgtattcggt gcgggtccac cgatggcggg gaagatcgag cgctaccacg ggtcgaacat
     1741 taccaagggg gtgcgtcatg gtgccgccga tgggtcagcg aggtcgacgg ctcagggaag
     1801 gtgggtggcg gggagcgctt cggggtcgat gccgtagcgc tggatggcgt cctggaccgt
     1861 ctccggcttc acatcgccgt gggcggcgag gcccgcgagc acggccacga tcacgtgggc
     1921 ggcgtccacc tcgaacaacc gacgcaatga ctcgcgagtg tcggagcggc ccatgccgtc
     1981 ggtgccgagc ggcgtgaaca cccgtccggg gaggaaccgg gcgacctgct ccggcacgat
     2041 cttcatgaag tcggtgacgg cgacgatcgg gccgggcgcc tcggtcagca gctcggcgac
     2101 gagtggggtg cgggcgggct gccccgggtg gagccggttc cagcgctcgg tgtcgagcgc
     2161 ctgttcccgg agcaccttgt aggaggtggc cgaccacagc tcgaccccga cgtcgtagtg
     2221 ctcggcgagc tgggcggcgg cctcacgtgc tgctccctgg gccgagcccg agaacagcac
     2281 cg
//
LOCUS       DATDBZ010000092         5876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_16940,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000092 DATDBZ010000000
VERSION     DATDBZ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16940"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VIX62_02185"
     CDS             <1..361
                     /locus_tag="VIX62_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEY5859077.1"
                     /translation="DYLKALAGEEYSAKDFRTWHATVLAAAEVAASPAATSTTAGRKV
                     VTETVKVVAGYLGNTPAVARASYIDPRVFDRYRDGITIGSVGNSFDLSSGERVRARVE
                     RAVLELLDHDAPAAAAA"
     gene            472..1035
                     /locus_tag="VIX62_02190"
     CDS             472..1035
                     /locus_tag="VIX62_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859078.1"
                     /translation="MERTVTRTWNDTADDVVVLGDATAPRSRIRWAAVFAGMVLSVGL
                     LVLLSSLWLAIAYGSDEAFVVRNLEWFVGASAVGCLLIGGFLAGRLSGVPGTGTGLAH
                     GLTLWALTLLVVLAIGIPSVINVLNLGRVATELQPTTSVIASGVDSSLWATFIAMVGA
                     LVAAGIGGMIGGASQRRRDHVEELDRR"
     gene            1032..1412
                     /locus_tag="VIX62_02195"
     CDS             1032..1412
                     /locus_tag="VIX62_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859079.1"
                     /translation="MNTRAERTTDDRNQRMDAIDDARAARLRDRLKRRLLVAAVAGPI
                     IGVVVGLLVGSVAFQVGGLGFVMVLVGCVIFATAVALLVASYSSLESPDPGLEPSQVE
                     RPIADRPGATRPEAEAPDDAVRGG"
     gene            1412..1663
                     /locus_tag="VIX62_02200"
     CDS             1412..1663
                     /locus_tag="VIX62_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859080.1"
                     /translation="MTSEGPETDAKRCPVCGRGVLVDIGFDADPAGGPEVPQQQPDAR
                     QVERYSCGHEVLGAALSTADGDVLDVERREASDVVDPGP"
     gene            1722..1952
                     /locus_tag="VIX62_02205"
     CDS             1722..1952
                     /locus_tag="VIX62_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859081.1"
                     /translation="MPDSMGKRQRRDVKAKKAAAIEERRVARAQRRADREAGLIQSGT
                     PLATEYGGTDLSKDPEPVPEDLLEADARRDDR"
     gene            1967..3343
                     /locus_tag="VIX62_02210"
     CDS             1967..3343
                     /locus_tag="VIX62_02210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859082.1"
                     /translation="MAVHQPAIPDRPPAWREVFQGRRGRLTAGLLLLEALVAVQVLIV
                     ATILPDVRRDLGMVQLYGLAFTASSLATLAAIPIAGRAVDRFGARATLPPVLFAFVGG
                     LVVAAIAPSMPVLLIGQFLQGAGGGGLYALSLGTVAKTYPDRLRPRVMALLATMWILP
                     GLVGPPVGAVIAGTVGWRWAFLAPIPVLALGWFLIAPALDLVPEPDLNADRLAVRWPL
                     QLMVGAGLVFTSLTIVRPWALAVLAAGLVVGLPALRHIVPPGTFRARRGIPATAIAAF
                     LLSMGFLAMDAFLTLMLTGVRGVSLGQASLAITAATLTWAAGSAWQSGRADRVALPKL
                     VLLGTIAVLVGQAAVASALFVEVPVVLAYAGWAVAGGGMGVAFPTIPLATMRVAGVGG
                     EAGVLSSVLLMDMLGVATGAGLGGGAVAVAEAFDAPLAAGIGASFAIGFAALIALAFV
                     ARRMTPET"
     gene            complement(3348..4316)
                     /locus_tag="VIX62_02215"
     CDS             complement(3348..4316)
                     /locus_tag="VIX62_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5859083.1"
                     /translation="MDPVLVIAAVAAVALVAVVVSALVRWRKRRVLIARYGAAAVGSR
                     RRPFRFRERLPVVLMVAGFACAVIAFAQTRIERQSTQGTVILTMDVSKSMQRTDVAPN
                     RLVAAQDAAEAFLSRLPGDFQVGVVTFADEATLAVEPTKDREEAGAALESTASSEGTV
                     IGDGMTASLDAIESTWAQGGRGPAAIVLLSDGRDTGSVVSPDEAAARAQEQGIPVFTV
                     TLGDATEGGSTASADDDLMLRIADGTGGSTFNALSADQLTDVYQELGSTLSSDLHVGS
                     SAWIFVLLGGVFALAAVVVLFMPATPAEYRAAPKPGSSKRPARSRS"
     gene            complement(4440..4772)
                     /locus_tag="VIX62_02220"
     CDS             complement(4440..4772)
                     /locus_tag="VIX62_02220"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY5859084.1"
                     /translation="MTDDDWTSTAAFDDLVDGRGVRVTVDDVGVLLVRSGERLYAIGS
                     RCTHQGAPLDRGPLRLTGSDPTVTCPAHGSVFRLQDGKVARGPAARPEPSFDTRVADG
                     IVQLRPQT"
     gene            4982..>5876
                     /locus_tag="VIX62_02225"
     CDS             4982..>5876
                     /locus_tag="VIX62_02225"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5859085.1"
                     /translation="MNETFAIQSLVLRDAIAGRDILARSRTGSGKTLAFAVPMVERLT
                     PSGRSPNALILVPTRELASQVTEEFRAIADVRHLTIAAVYGGVGIGPQAKRARHADIV
                     IATPGRLLDLVSRKLLRLDKVRMVVLDEADRMLDMGFLPDVNRILEQLRPERQTMLFS
                     ATLDGEVGRLAKRFTTDPVLHEIIDDSPAAPDALHRFIAVNQPDKTRALVRELAAERG
                     LTLIFVRTQRGADRLSRNLKQSGFHVGSLHGGMSQPQRERALGQFASGHKDVLVATDV
                     AARGLDLEDITHVINFDAPADD"
BASE COUNT          827 a         2042 c         2097 g          910 t
ORIGIN      
        1 cgactacctc aaggccctcg cgggcgagga gtactccgcc aaggactttc ggacctggca
       61 cgccacggtg ctcgcggcgg ccgaggtcgc ggcgtcgccg gcagcaacgt cgacaacggc
      121 tggtcgcaag gtggtcacgg aaacggtgaa ggtcgtcgcc ggctacctgg ggaacacccc
      181 cgcggtggca cgggcctcct acatcgatcc gagggtcttc gatcggtacc gcgacggcat
      241 cacgatcggg tcggtaggca attcgttcga cctgtccagc ggggaacgag ttcgggcacg
      301 ggtcgaacgt gccgtgctcg aactcctcga ccacgacgcg ccggccgccg ccgcggcgtg
      361 accgccgccg agatcgcaga ggttgccgga cgtgtagatc accgcgcgat cgggaatgcg
      421 tctcctacag gcaggcccgg gccagaccgg ggtacgccag gaggtgcagt catggaacga
      481 acggtcacga gaacgtggaa cgacacggct gatgatgtcg tcgtgctggg cgatgccacg
      541 gcgccacgat cccggatccg atgggccgcc gtgttcgcag ggatggtgct gtcggtgggc
      601 ctgttggtct tgctgtcctc actgtggttg gcgatcgcct acgggtccga cgaggccttc
      661 gtcgtacgca acctcgagtg gttcgtgggt gccagcgccg tcggctgtct cctgatcggc
      721 gggttcctgg ccgggcggtt gtccggggtt cccggaaccg gaaccggcct cgcacacgga
      781 ctgacgctgt gggccctgac cctgttggtg gtcttggcga tcggcatccc gtcggtgatc
      841 aacgtactga acctgggacg cgtggccacc gagctccagc cgacgaccag cgtcatcgcg
      901 tccggtgtgg acagcagcct gtgggcgacg ttcatcgcga tggtcggtgc gctcgtggcc
      961 gcggggatcg gcggcatgat cggcggcgcg tcgcagcgtc ggcgcgatca cgtcgaggag
     1021 ctcgatcggc gatgaacacg cgggccgaac gaacgacgga cgaccggaac cagcggatgg
     1081 acgcgatcga tgacgcccgg gccgcccggc tgcgggaccg gctgaagcgc cggctcctgg
     1141 tcgcagcggt ggccgggccc atcatcggcg tggtggttgg gctcctcgtg ggctcggtcg
     1201 ccttccaggt cggtgggctc ggcttcgtga tggtgctggt tggctgcgtc atcttcgcca
     1261 ccgccgtggc gctcctggtc gcgagctact cgtcgctcga atcgcccgac cccggcctcg
     1321 aaccgtcgca ggtcgagcgt cccatcgcgg accgtcccgg ggccacgagg cccgaggcag
     1381 aggcgcctga cgacgccgtc cgggggggct gatgacctca gagggacccg agacggacgc
     1441 gaagcgatgt ccggtgtgcg gtcgtggcgt cctggtcgac atcgggttcg atgccgatcc
     1501 cgccggtggt cccgaggtcc cgcagcagca gccggacgcg cgacaggtgg aacgctattc
     1561 ctgcggccat gaggtgctcg gcgcggccct gtcgaccgcc gatggagacg tgctggacgt
     1621 cgagcgccgg gaagcctccg acgtggtcga tcccggaccg taacgcggtt ggcggatcac
     1681 ctggcggtgg cgtccacccg cgtttctcgt aggctgggtc catgccggac agcatgggca
     1741 aacgccaacg ccgtgatgtg aaggccaaga aggccgcagc catcgaggag cgccgtgtcg
     1801 cacgcgcgca gcgccgtgcc gaccgcgaag ccggcctgat ccagtcgggc acaccgttgg
     1861 ccaccgagta cggcggcacc gacctcagca aggatcccga accggtcccc gaggatctcc
     1921 tcgaagccga cgccagacgc gacgaccggt agcgtcgtcc acggcgatgg ccgtccacca
     1981 gcccgcgatc cccgaccggc ctccggcgtg gcgtgaggtc ttccagggcc ggcgtggacg
     2041 cctcaccgcg ggcctcctgc tcctcgaagc gctggtcgcc gtccaggtcc tgatcgtcgc
     2101 cacgatcctg cccgatgtgc gccgtgacct gggcatggtg cagctctacg gactggcgtt
     2161 caccgcctcg agcctggcga ccctggcggc gatcccgatc gcagggcgcg ccgtcgaccg
     2221 gttcggagcc cgcgccaccc tgccgcccgt gctgttcgcc ttcgtcggcg gcctggtggt
     2281 ggccgcgatc gcaccctcga tgcccgtcct gctcatcggc cagttcctcc agggcgccgg
     2341 gggtggaggt ctctacgcgc tgtcgctcgg caccgtagcc aagacctatc ccgaccggct
     2401 gcgaccacgg gtgatggcgt tgctggccac gatgtggatc ctcccgggcc tggtcggtcc
     2461 cccggtcggg gccgtgatcg ccggcacggt ggggtggcgc tgggccttcc tggccccgat
     2521 ccccgtgctc gcgctggggt ggttcctcat cgcgccggcg ctcgacctcg tgccggaacc
     2581 ggacctgaac gccgatcggc tcgcggtgcg atggccgctg cagctgatgg tgggggcggg
     2641 cctggtgttc acctccctca cgatcgtgcg cccgtgggca ttggccgtgc tggcggcagg
     2701 gctcgtggtc gggctaccgg cactgcgcca catcgtgccg cccggcacct tccgggcacg
     2761 tcgcgggatc cccgcgacgg cgatcgcggc gttcctgcta tcgatggggt tcctggcgat
     2821 ggacgccttc ctgaccctga tgctgacggg ggtgcggggc gtctcgttgg ggcaggcgag
     2881 cctggcgatc acggcggcga cgctgacgtg ggccgccggg agcgcctggc aatcgggccg
     2941 agccgaccgt gtggccctcc cgaagctggt gctgctgggg acgatcgccg tcctcgtggg
     3001 tcaggccgcg gtggcgagcg ccttgttcgt cgaggttccc gtggtgctgg cctacgcggg
     3061 gtgggcggtc gcgggtggcg ggatgggggt ggccttcccg acgatcccgc tggccacgat
     3121 gcgtgtcgcc ggcgtgggcg gggaggccgg ggtcctgtcc tcggtgctgc tcatggacat
     3181 gctcggggtc gcgaccgggg ccggcctcgg agggggcgcc gtcgccgtgg ctgaggcctt
     3241 cgatgcgccg ctggccgctg ggatcggtgc gtcgttcgcg atcgggttcg cggcgctgat
     3301 cgcgctggcg ttcgtcgcgc ggcggatgac gcccgagacc tgagccgtca ggaccgggaa
     3361 cgcgcgggac gcttcgacga tccaggcttc ggtgccgccc ggtactcggc cggggtcgcg
     3421 ggcatgaaga gcaccaccac cgcagcgagc gcgaacacac cgccgagcag cacgaagatc
     3481 cacgcgctgc tgccgacatg caggtccgag ctgagggtcg acccgagctc ctgatagacg
     3541 tcggtgagct ggtcggcgct gagcgcgttg aacgtgctcc cgcccgtccc gtcggcgatg
     3601 cgcaacatga ggtcgtcgtc ggcgctcgcc gtggaaccac cctcggtggc gtcgcccagc
     3661 gtcacggtga acaccgggat gccctgctcc tgtgcacgcg ccgccgcttc gtccggcgac
     3721 accacgctcc cggtgtcacg cccatcggac aggagcacga tcgccgccgg tccgcggccc
     3781 ccttgggccc atgtggactc gatcgcgtcg agggaggccg tcatcccgtc cccgatcacg
     3841 gtcccttccg acgacgcggt cgactcgagg gcggcgccgg cttcctcacg gtccttggtc
     3901 ggctcgacgg cgagggtcgc ctcgtcggcg aacgtcacca cccccacctg gaagtcgccg
     3961 ggcaaccgcg acaggaacgc ctcggcggcg tcctgagcgg ccaccagtcg gttcggcgcg
     4021 acgtcggtcc gttgcatcga cttcgacaca tccatcgtca ggatcacggt tccctgggtg
     4081 ctctgacgct cgatccgggt ctgggcgaac gcgatcaccg cgcaggcgaa gccggccacc
     4141 atcaggacga cgggcagccg ctctcggaac cggaacggcc tgcgtcgcga tccgaccgct
     4201 gcggcgccgt agcgggcgat caggacccgt cgcttgcgcc accggacgag cgccgacacc
     4261 accacggcca cgagcgcgac cgcggcgacc gcggcgatga cgaggacggg atccatcagc
     4321 gggacgatcc tgggtacggc gagacgccag ccatacatga cctatcgacc gcaagcacgc
     4381 gcgtgttgag tgccgaaccg aaggaagtgc cggtggtccc gggtctcagg tctggcgcct
     4441 caggtctggg gacggagctg cacgatgccg tcggcgacac gggtatcgaa cgacggctcc
     4501 ggtcgtgcgg cgggccctct cgccaccttg ccgtcctgga ggcggaacac gctcccgtgg
     4561 gccgggcagg tcaccgtggg atcggatccg gtgagccgga gcggcccccg gtcgagcggg
     4621 gctccctggt gcgtacagcg actgccgatc gcgtacaacc gctccccgga tcgcaccaac
     4681 aggacgccga catcgtccac ggtgacgcga acgcccctgc cgtccacgag gtcatcgaag
     4741 gccgccgtgc tcgtccagtc gtcgtcggtc atcgcgacac ggtcggacac gtgagggcac
     4801 ctgtcaaccg cgtcgcgggc gatggcgggc gggattcccg tactgtgggt gtatgggtca
     4861 gcagccgcgc gcggcacaac ggtccgcgcc gacggctctc gccgcctccg cgatcgccac
     4921 acgtccgttc accgaattcg gcgtgtccgc acccgtcgtc gaggcactgc ggatgcgggg
     4981 gatgaacgaa accttcgcga tccagtcgtt ggtgctgcgc gatgcgatcg cagggcgcga
     5041 catcctcgcg agatcacgca cgggatcggg caagaccctg gcgttcgccg tgccgatggt
     5101 ggaacggctc accccgtcgg gccgcagccc caacgcgctg atcctggtcc ccacccgcga
     5161 gctcgcgagc caggtcaccg aggaattccg cgcgatcgcc gacgtgcgtc acctcacgat
     5221 cgcggcggtg tacggcggcg tgggcatcgg tccacaggcc aagcgggcac gccacgccga
     5281 catcgtgatc gccacacccg gacgcctcct cgaccttgtg tcgcgcaaac tgctccggct
     5341 ggacaaggtg cggatggtcg tgctcgacga ggccgatcgc atgctcgaca tgggcttcct
     5401 gccggacgtg aaccggatcc tcgaacagct ccggcccgaa cggcagacga tgctgttctc
     5461 ggcgacgctc gacggcgagg tgggtcggct cgcgaagcgc ttcaccaccg acccggtgct
     5521 gcacgagatc atcgacgact cccccgcggc gcccgatgcc ctccaccgct tcatcgcggt
     5581 gaaccagccc gacaagaccc gcgccctggt gcgagagctc gccgccgaac gcggcctcac
     5641 gttgatcttc gtgcgcaccc aacgcggcgc ggaccgcctg tctcgcaacc tcaagcagtc
     5701 agggttccat gtggggtcgt tgcacggagg catgagccag ccgcaacggg aacgcgccct
     5761 gggacagttc gcctccgggc acaaggatgt gctggtggcg accgacgtcg ccgcccgcgg
     5821 tctcgacctc gaggacatca cccacgtcat caacttcgat gctccggccg acgaca
//
LOCUS       DATDBZ010000093         3418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_850008, whole genome shotgun sequence.
ACCESSION   DATDBZ010000093 DATDBZ010000000
VERSION     DATDBZ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850008"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..626)
                     /locus_tag="VIX62_02230"
     CDS             complement(42..626)
                     /locus_tag="VIX62_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859086.1"
                     /translation="MWFVEVEPAAQGAATVSIGVEGDELVVSLPHTYWEIWRSKRGRS
                     VLEVLASDLEAIFAGRIEEVGLAPTDSRICCGRMERSGAWDRPISRCRGGGGTDRGTA
                     HTARLGAGTDAACGHHNLEGLGDIAGVLERANPKERAKVYADLGVTRIYRPDVGLVDV
                     QAVPVAACTYERVGGAMTPIRTRELRGVIDLKAA"
     gene            911..1150
                     /locus_tag="VIX62_02235"
     CDS             911..1150
                     /locus_tag="VIX62_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859087.1"
                     /translation="MEGIEAVAAVEAPADIQGRERWYIASQTGGLWVVTTLDDSASGL
                     ILPLNNRAVTRASWGLTPHGMLPCPVTQPPTAARQ"
     gene            complement(1193..2542)
                     /locus_tag="VIX62_02240"
     CDS             complement(1193..2542)
                     /locus_tag="VIX62_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5859088.1"
                     /translation="MARRLPERVAEIKVELCQVRRSTLATPRRRLTRDDSGHALRAGT
                     HAAPLGGVMLLAARRVFTGQSSEVLEDRVLVIEQGRIVDIGDRSSASGADLVDLGDVT
                     ILPGLIDVHQHLAFDASSDPVAQLDADDDLTLLLRMRLAALRALSVGITTIRDLGDRS
                     YLSLTLRDWFAAGAEAGPRIITSGPPITTRNGHCWFLGGEVDDAEAIRAAVRDRAARG
                     VDVIKVMASGGNLTPTVGPHESQFGRGELAIAREESHRVGLSLAVHAHGGQAVVDALA
                     VGADSIEHCTFFSAEGVEVDPGILEQLAASDCVISMTAAVVPGNAAAYPAMQQRLAAI
                     VANHATLYRLGGRVVCSSDAGVVPSKPHTALPHGVSTFLPSIGMSNAEAIMNVTAVAA
                     QVCGLADRTGTLEPGHDADLLAVAGNPLEDITAIHDVVAVFARGKPLQTPGHRPDPR"
     gene            2706..2996
                     /locus_tag="VIX62_02245"
     CDS             2706..2996
                     /locus_tag="VIX62_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859089.1"
                     /translation="MTRPVPEEWQGLSQEENDAKTAAILDAVAKNGGDIKVTAFSGDH
                     MALVSVIEYPDEMSGRQAVADIIAIGTLEFVSHHSLWDLGEWTTMLREAQGT"
BASE COUNT          594 a         1160 c         1146 g          518 t
ORIGIN      
        1 acgactgaga cgaggagcgc gccaacgtcg gtttatgagg attacgccgc cttcaggtcg
       61 atcacacccc ggagctcgcg tgtgcgtatg ggagtcatcg cccctccgac acgctcataa
      121 gtacacgcgg cgaccgggac cgcctgaaca tcgactaggc ccacatcggg cctgtagatg
      181 cgggtcacgc cgaggtcggc gtacaccttc gcccgctcct tcgggttggc ccgctcgagc
      241 acacctgcga tgtctcccag gccctcgaga ttgtggtggc cacaagctgc gtcagtgccc
      301 gcccccagcc gcgccgtatg agcggtacct cgatcggtgc cgccaccgcc gcggcaccgg
      361 gagatgggcc gatcccacgc tccggatctt tccatccggc cacagcagat gcgcgaatcg
      421 gtgggagcca aacccacctc ctcgatccgc ccagcgaaga tcgcctccaa gtctgaggcg
      481 agtacctcca acaccgaccg gccgcgcttt gagcgccaga tctcccagta cgtgtgcggg
      541 agcgatacga cgagctcgtc gccttccacg ccgatcgaga cagtcgccgc gccttgggca
      601 gcaggctcga cctcgacgaa ccacatgccg gccctcggac tccagacagt cgcgatggaa
      661 gctgccgccg tcccgtacgc gacgagctgc gctcgcacaa cgctttcgac ttcatcgcag
      721 caggccgttc aggcaagtcg cgggaaatcg agtgccgccg gctgcctcgc cgtcatcctc
      781 gttgtcctcg tgcttgcagt catcggtgcc ctggccgggg gaggcgatga caacaccgac
      841 accaacacag cacggccggt acggagaaca acgacccctg gatccgacct gccgcaccat
      901 cacctactcg gtggaaggca tcgaggccgt ggccgccgtg gaggcccctg ccgacatcca
      961 gggcagggaa cggtggtaca tcgcctcaca gaccggcggg ctctgggtgg tcaccacgct
     1021 ggacgactcc gccagcgggc tgatcctgcc gctaaacaat cgagccgtca cgagagcgtc
     1081 atggggattg acgccccacg ggatgctccc gtgtccggtg acgcaaccgc cgacagcggc
     1141 caggcaatag acgccctcgc gtgtgcgcag ggcgggtgac gacagaagac cgtcagcgcg
     1201 gatccggccg gtggcctggc gtctgaagtg gcttgccgcg tgcgaatact gcgacgacgt
     1261 cgtggattgc ggtgatgtcc tcgagtgggt ttccggcgac agcgaggagg tcggcgtcgt
     1321 ggccgggctc gagggtgccg gtgcggtcgg cgaggccgca cacctgggcg gcgacggcgg
     1381 tcacgttcat gatcgcttcg gcgttgctca tgccgatgga cgggaggaac gtgctgacgc
     1441 cgtggggaag cgcggtgtgc ggcttgctcg gcacgacccc ggcatcgctg ctgcacacga
     1501 cgcggccgcc gagacggtag agggtcgcat ggttcgccac gatcgccgcg aggcgctgtt
     1561 gcatggccgg ataggcggcg gcattgccgg gcaccaccgc ggcggtcatc gagatcacgc
     1621 agtcgctggc ggcgagctgt tcgaggatcc ccgggtcgac ctcgactccc tcggcggaaa
     1681 agaaagtgca atgctcgatc gagtccgcgc ccacggcgag agcgtcgacc acggcctgtc
     1741 cgccgtgcgc gtgcaccgcc agggacagtc cgacccggtg cgactcctcc cgtgcgatgg
     1801 ccagctcgcc gcgaccgaac tgggactcgt ggggcccgac ggtcggggtc aggttgcccc
     1861 cgctggccat caccttgatc acatccacgc cccgggccgc ccgatcccgc acggccgcgc
     1921 ggatcgcctc cgcgtcgtcg acctcgccgc cgaggaacca gcagtgaccg ttgcgggtcg
     1981 tgatcggcgg ccccgaggtg atgatgcgag ggccggcctc ggcaccggcg gcgaaccagt
     2041 cccgcaacgt gagcgacagg tagctgcggt cacccaggtc acggatggtc gtgatcccga
     2101 ccgacaacgc ccgtagcgca gccaaccgca tgcgcaggag cagcgtgagg tcgtcgtcag
     2161 catcgagctg ggccaccggg tcgctcgacg cgtcgaacgc gaggtgctga tggacgtcga
     2221 tgagccccgg aaggatcgtc acgtcaccaa gatcgacaag atcggcgccc gacgcgctcg
     2281 accggtcgcc gatgtcgacg atgcggccct gctcgatcac cagcacccgg tcctcgagca
     2341 cctcgctcga ttggccggtg aagacccggc gggcggcgag gagcatcacc cctcccagcg
     2401 gtgctgcgtg cgtgccggca cgtagtgcat gacccgaatc gtcgcgcgtc agcctgcgcc
     2461 gaggagtggc cagtgtggac ctgcggactt ggcagagctc gactttgatc tcggcgaccc
     2521 gctcgggcaa gcgcctcgcc acctcccgat ggaacagacc ggtgtgccgt gcatcgacga
     2581 cggcatgggc tcggtgacac aggagggcga gacatcgtga tccgcattac ggtcgtgccg
     2641 gtgtcgggcc gctcagaccc gcgtggtagt aggggaggat ggcaatgggc tacatctcat
     2701 ttgccttgac caggccggtg cccgaggagt ggcaggggct gagtcaggag gagaacgacg
     2761 ccaagactgc ggcgattctt gacgcggtcg ccaagaacgg cggggacatc aaggtcaccg
     2821 ccttctcggg ggaccacatg gcactggtgt cggtcatcga gtaccccgac gagatgtcgg
     2881 gtcgacaggc cgtggccgac atcatcgcga tcggcaccct ggagttcgtc agccaccaca
     2941 gtctgtggga cctgggcgag tggacgacaa tgctccgcga ggcacagggc acctgatcga
     3001 cggaggtttg cgggccctca tgcagccatg ccgccgggcc agcttgccct ccgtcgcgag
     3061 caagccatcg acctcctcga ggaactgacc caactacgag gacggcacga ggcgctgcgc
     3121 gaggccctac gacgcgtggc cgatgaagac taaaagatcg acctgctgcc gtgtcaggca
     3181 acggtcgccc tgccccccac cggcatttcg aggacaccca caggtcgcgg tcgagcagcc
     3241 ctcagcgggg ttgcgcgtcc ccgaagatga accagggcgt tgcgccgatc ttcgcagtcc
     3301 aaccctcttg gtcgagcagt gaccgcagtt cgtcgggctc gtagaagatc tcaacgacct
     3361 ggtaccgcct tccgtcgtgc aggcggcgca gatcaagatc cggtccagcc cgcatcac
//
LOCUS       DATDBZ010000094         4129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_850168, whole genome shotgun sequence.
ACCESSION   DATDBZ010000094 DATDBZ010000000
VERSION     DATDBZ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850168"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VIX62_02250"
     CDS             <1..277
                     /locus_tag="VIX62_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859090.1"
                     /translation="SADEDETGTSPGLEHLGWNVIVRDEGTCDTLGSGRPNRPFIAGD
                     TSAQAPAPAVSREEEGGGLGWLWFVAVAVVLAIGRVVHLLFLKRAGG"
     gene            complement(287..1144)
                     /locus_tag="VIX62_02255"
     CDS             complement(287..1144)
                     /locus_tag="VIX62_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY5859091.1"
                     /translation="MTRLLHVVGAQVSPVAGDPEATVDKFAHDVRMMRGSFPKAQLFT
                     FPELYLTGEHPFRHHVAMDAIRHRVPGPFTDRIGEVARRARRWIQAGSIYEAAGRKTY
                     NTALLFAPDGSLAGTYRKLFPWMPLETSDPGDAPPPVVAMPRLGAAGMMICYDGWFPE
                     VARSLAMRGAEVILHPTLTTTPDREEELVMARANAIANQCYVINVNASVEIGGGRSIG
                     VDPEGRVLFEGGSGEEFFSEVLDLDRVATVRDRGTRGMNRLLWQLQEHGPKAADPNYR
                     SFRADRPTR"
     gene            complement(1187..2635)
                     /locus_tag="VIX62_02260"
     CDS             complement(1187..2635)
                     /locus_tag="VIX62_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEY5859092.1"
                     /translation="MDSHPPSSNVRPGHRDPHAHAADQPFEYPQQREFFEPDWTRLPG
                     YRDVTKDQWESAQWQRAHTVKNLKEFAEALGHHLDPELLEDIRRDQEERATMSMLLPP
                     QMINTMREDDLRSDPVRRYMAPALSERETEWPSHPLSSRDSLHEADMWAVEGLTHRYP
                     TKVLAELIPTCPQYCGHCTRMDLVGNDTLQVLKYKFETKQNERWDQMLDYLRATPQVR
                     DVVVSGGDMANVPVKRLEAFVMSLMDIDNIRDIRIATKGLMGLPQHFLQDDVRAAFER
                     MGARANERGVDIAVHTHVNHANSVTPLVRDAAQMVLDSGFRDVRNQGVLLRGVNTTVN
                     ELLDLCFTLLDHGKILPYYFYMCDMIPNAEHWRTSVAETQALQFGLLGYMPGFATPRM
                     VCDVPFVGKRWVHMLGDYDRTRGISYWTKNYRTGIEFDDPEALSRQYHYYDPIYTLPE
                     EGQEWWRAKMREGLDDLLAELATEQGLEVGAS"
     gene            complement(2822..3097)
                     /locus_tag="VIX62_02265"
     CDS             complement(2822..3097)
                     /locus_tag="VIX62_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859093.1"
                     /translation="MADYREILHERLYVRVPGTDKLRKTAANRHKHLLANGWRELERS
                     QAADHIMVKYERTGHAPMKARLPKQGPEIRIERRPRRDDNRRGGGRR"
     gene            3227..>4129
                     /locus_tag="VIX62_02270"
     CDS             3227..>4129
                     /locus_tag="VIX62_02270"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5859094.1"
                     /translation="MGASARGEAALATVASAVEALLLSEGRIERLDELVAGVGAAVEA
                     DRAFVFQNVRSPDGRLWMDLVGEWDADGIRQIFEDPDNHLHPYAPDFSRRIDVYGKGE
                     PVVGLVSDLPEPERTVLAAEDVVSVASVPIMVGSEWWGFLGFDDCSEGRVWDEVDLEA
                     LWTTAQALGVMLQRDLDEETRRFSEDQFRSMVEEGPAVVYIDGPDEGASSIYISPQIE
                     RVLGYSPDEWYADPDLWGKVLHPDDRSRALAENERHNETGDPFVMEYRMFHKGGHVVW
                     VHDEATMIRDDRGVPRFSHGVMMDI"
BASE COUNT          633 a         1316 c         1482 g          698 t
ORIGIN      
        1 gtccgccgat gaggacgaaa cgggcacctc gcccgggctc gagcacctcg gctggaacgt
       61 gatcgtgcgg gacgagggca cctgcgacac cctgggcagc ggccggccga accgcccctt
      121 catcgccggt gacacctccg cgcaggcacc ggcgccggcg gtctcccgcg aggaggaagg
      181 cggcggcctg ggttggctgt ggttcgtcgc ggtcgccgtg gtgctcgcga tcggccgcgt
      241 ggtgcatctg ctgttcctga agcgcgcggg cgggtagccg cggacgtcag cgcgtggggc
      301 ggtccgcccg gaacgaccgg tagttcggat cggccgcctt cgggccgtgt tcctggagct
      361 gccagagcag ccggttcatc cctcgtgtgc cgcgatcacg gaccgtggcg acccggtcga
      421 ggtcgagcac ctcgctgaag aactcctcgc cgctgccgcc ctcgaacaac acccgcccct
      481 cggggtcgac gccgatcgag cggcccccgc cgatctcgac gctggcgttg acgttgatca
      541 catagcactg gttcgcgatc gcgttggcgc gcgccatgac gagctcttcc tcacggtccg
      601 gggtggtggt cagcgtggga tgcaggatca cctcggcgcc gcgcatcgcg agcgacctcg
      661 cgacctccgg gaaccacccg tcgtagcaga tcatcatgcc cgccgcgccg aggcgaggca
      721 tcgcgacgac gggcggaggt gcgtcgccgg ggtcgctcgt ttccagcggc atccacggga
      781 agagcttgcg gtaggtgccg gcgagcgagc catcgggcgc gaagagcagc gcggtgttgt
      841 acgtcttgcg cccggcggcc tcgtagatcg acccggcctg gatccagcgg cgagcccgtc
      901 gcgcgacctc gccgatccgg tccgtgaacg gccccgggac ccggtgacgg atcgcgtcca
      961 tcgccacatg gtggcggaac ggatgctcac cggtgaggta gagctcgggg aacgtgaaca
     1021 gctgcgcctt cgggaaggag ccgcgcatca tccgcacgtc gtgcgcgaac ttgtccacgg
     1081 tggcctcggg atcgccggca acgggcgaca cctgggcgcc cacgacgtgc agcaaccggg
     1141 tcatgcaggc gacgctagca gagggccgga ccgagggacc gggcgctcag gaggcgccga
     1201 cctcgagccc ctgttcggtg gcgagttcgg cgagcaggtc gtcgagtccc tcgcgcatct
     1261 tcgcgcgcca ccactcctgt ccctcttcgg gcagcgtgta gatcgggtcg tagtagtggt
     1321 actggcgcga caacgcctcg ggatcgtcga actcgatccc cgtgcggtag ttcttcgtcc
     1381 agtaggagat cccgcgtgtg cggtcgtagt cgcccagcat gtgcacccag cgcttgccca
     1441 cgaaggggac gtcgcacacc atccgagggg tcgcgaaccc gggcatgtag cccagcagac
     1501 cgaactgcag cgcctgggtc tcggcgacgc tggtgcgcca gtgctcggcg ttggggatca
     1561 tgtcgcacat gtagaagtag tagggcagga tcttgccgtg atcgagcagg gtgaagcaca
     1621 ggtcgagcag ctcgttcacg gtggtgttca caccacggag cagcacgccc tggttccgca
     1681 cgtcgcggaa gccggaatcg agcaccatct gcgccgcgtc gcgcacgaga ggtgtcaccg
     1741 agttcgcgtg gttcacgtgg gtgtggaccg cgatgtcgac accgcgctcg ttcgcgcggg
     1801 cccccatacg ctcgaaggcg gcacggacgt cgtcctgcag gaaatgctgg ggcaggccca
     1861 tcaggccctt ggtcgcgatc cggatgtcgc ggatgttgtc gatgtccatg agtgacatca
     1921 cgaaggcctc gaggcgcttg accggcacgt tggccatgtc gccgccgctc accacgacgt
     1981 cgcggacctg cggggtagcc cggaggtagt ccagcatctg gtcccagcgc tcgttctgtt
     2041 tggtctcgaa cttgtacttc agcacctgca gggtgtcgtt gcccacgagg tccatccggg
     2101 tgcagtgccc gcagtactgg gggcacgtgg ggatcagctc ggcgagcacc ttggtggggt
     2161 atcgatgggt gagcccttcg accgcccaca tgtcggcttc atgcaatgag tcccggctgg
     2221 acaacgggtg gctgggccat tccgtctcgc gttcgctgag cgcgggcgcc atgtagcggc
     2281 ggaccgggtc ggagcggagg tcgtcctccc gcatcgtgtt gatcatctgc gggggcagca
     2341 gcatcgacat cgtggcgcgc tcttcttggt ctcgccggat gtcctcgagc aactcgggat
     2401 ccaggtgatg gccgagcgcc tcggcgaact ccttgaggtt cttcacggtg tgggcgcgct
     2461 gccactgggc ggactcccac tggtccttcg tgacgtcccg gtagccgggg agccgggtcc
     2521 agtcgggctc gaagaactcg cgctgctgtg ggtattcgaa tggctggtcg gccgcgtgcg
     2581 cgtgcgggtc gcggtggccg ggacggacgt tggacgacgg cggatgggag tccacgcttc
     2641 tcacggatga caccccctgg ttcgtgcgaa cgcgccgcgg ggttcgcggc gcgcgcttcc
     2701 ggacaactca atggttgttc ctcatcatac ccgaagaacg ttcggcagaa ctagggttga
     2761 accgtaggat gttcggtcac ctggcggaca cggcaggcgt ggcagggaac gaagaggtgg
     2821 ctcagcgcct gccgccgcca cgacgattgt cgtcgcggcg aggacggcgt tcgatgcgga
     2881 tctcgggccc ctgcttgggc aggcgagcct tcatgggggc atgaccagtg cgctcgtact
     2941 tgaccatgat gtggtcggcg gcctggctgc gctccaactc acgccatccg ttggccagca
     3001 agtgcttgtg gcggttggcg gcggtctttc gcagcttatc cgtgcccgga acgcgcacgt
     3061 acaggcgttc gtgcaggatc tctcggtagt cagccatcgc ggacaagggt agcaggcgac
     3121 cgacgcgttt cggttcccga acggtgggcg cacccttcac gacctgcgga aaaggctttg
     3181 ccgcaacgtc aaggcgcggc cggcaagggc cgatgaccaa ggcatcatgg gggcttctgc
     3241 gcggggagag gcggcactgg cgacggtcgc atcggccgtg gaagcattgc tgctcagtga
     3301 gggccggatc gagcggctgg atgaactggt cgcgggcgtc ggcgccgccg tcgaggccga
     3361 tcgagcgttc gtgttccaga acgtgcggtc ccccgacggt cggctgtgga tggatctggt
     3421 cggggagtgg gatgcggacg ggatccgcca gatcttcgag gatcccgaca accacctcca
     3481 cccgtacgcc cccgacttct cccgtcgcat cgacgtctac ggcaagggag agccggtcgt
     3541 cggtctcgtc agcgaccttc ccgagccgga gcgcaccgtg cttgcggccg aggacgtcgt
     3601 gtccgtggcc tcggtgccga tcatggtggg ctcggaatgg tggggcttcc tggggttcga
     3661 cgactgctct gagggacggg tctgggacga ggtggacctg gaggcgttgt ggaccaccgc
     3721 acaggccctg ggcgtgatgc tccagcgaga cctggacgaa gagacgcgcc ggttcagcga
     3781 ggaccagttc cgatcgatgg tggaggaagg cccggcggtc gtctacatcg acgggccgga
     3841 cgagggcgcc tcctccatct acatcagccc ccagatcgag cgcgtgctcg gctacagccc
     3901 ggacgagtgg tacgccgacc ccgacctgtg gggcaaggtg ctgcaccccg acgaccgctc
     3961 ccgcgccctc gccgagaacg aacgccacaa cgaaacgggt gacccgttcg tgatggagta
     4021 ccgcatgttc cacaagggcg ggcacgtggt gtgggtgcac gacgaagcca cgatgatccg
     4081 ggacgaccgc ggggtgcccc ggttctccca cggcgtgatg atggacatc
//
LOCUS       DATDBZ010000095         7040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2897411, whole genome shotgun sequence.
ACCESSION   DATDBZ010000095 DATDBZ010000000
VERSION     DATDBZ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2897411"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /gene="mraZ"
                     /locus_tag="VIX62_02275"
     CDS             <1..452
                     /gene="mraZ"
                     /locus_tag="VIX62_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY5859095.1"
                     /translation="VEERGGGVAELLGTHSYQLDPKGRVSLPARFRAAFVDGAWLTVG
                     QDRCLYCFPRAEWERRADEVAAFPLSDNDGRAYSRLFFGASEEVKLDGQGRVTIPQRL
                     REVVGIDKEVVVLGVRDRMEIWDREVYDAYAAGFADAYQTGALDPRR"
     gene            457..582
                     /locus_tag="VIX62_02280"
     CDS             457..582
                     /locus_tag="VIX62_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859096.1"
                     /translation="MAAPLGPKLGTEVRQSLSLIGMTALTLLASIGLGILAGHIG"
     gene            587..1462
                     /gene="rsmH"
                     /locus_tag="VIX62_02285"
     CDS             587..1462
                     /gene="rsmH"
                     /locus_tag="VIX62_02285"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY5859097.1"
                     /translation="MDALPPNEDAPHVPVMVAEVTGFLRDRGTVVDMTLGAGGHARAL
                     LESGVERVIGIDRDRSAITLATERLAPYGSRFRAVETTFSGVDHELTDGAVDGILFDL
                     GVSSMQLDTAERGFGYRKDGPLDMRMGGDGPSAADLVNELSEPELADLIYEYGGEHRS
                     RRIARAIVRKRPITTTDQLAGVIAGALGKRPGGPHPARRTFQALRIAVNRELDELAAS
                     LPRAVELLAPGGRVVVIAYHSLEDRIVKRCFREHEQVLILTKKPLRASEDEVAANPRA
                     RSAMLRAAERVEVAA"
     gene            1618..1887
                     /locus_tag="VIX62_02290"
     CDS             1618..1887
                     /locus_tag="VIX62_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859098.1"
                     /translation="MFSAILVAGLVLGVVALNALVVNTTYRLEAVEQRQRAAAESGAA
                     LEVQVAHQSSPSRISRWAGAHGMVLPDSDAVVPLQVAGPRVGDAG"
     gene            1884..3608
                     /locus_tag="VIX62_02295"
     CDS             1884..3608
                     /locus_tag="VIX62_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY5859099.1"
                     /translation="MTARGPKNPPVHRLVAMLAVMLLALGAIVVRLAFLQVGDNGQFA
                     EMGLQQRVRTVDLPAHRGEILDRFGMPLALTRAAKDIYVNPTLVEDPEGEAATIADIL
                     SMKQKDVEQALESDGTFVYLQRQVDLDIADELEAQQLPGIGFLEVPQRYYPAGSLAPQ
                     VLGLVDVDGKGISGLETQYQTQLAGTPGERTAELSADGLPISSGLDTLTEPVPGTTVV
                     TTIDRQMQFMAQTALERAVQANGALGGTVVVMDPHTGDVYAMATYPWFDPNDFSGASQ
                     DAMRNRAVTDSFEPGSVNKIITAAAALETGSVSLDQRFRVPSSMQVGPFTIYDSHVHP
                     IETMTLGDIIAESSNIGAALVANEVGNDQMGAYMQRFGYGQPTGIGFPGEASGVLLPA
                     SQWDEVIRATVSYGQGISVTPMQMADVYATIANGGRWVQPRLVRGFEGPDGTFSDAPE
                     QRTRRVVSPTTADLLTRMLAYVVEDGTGAEAQIPGYQVAGKTGTSRKLDDSGHYVQRY
                     MASFVGFLPASDPRVVIAVSIDEPRTVYGGLAAAPLFQEIARYAIQRLSIPASPPVAL
                     PPHAQELP"
     gene            3654..5114
                     /locus_tag="VIX62_02300"
     CDS             3654..5114
                     /locus_tag="VIX62_02300"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY5859100.1"
                     /translation="MPPSFPQRLFADVLGAVPEAEVRGDAIIGDVAFDSRDVTPGALF
                     FCVPGATVDGHDFAMAAIEAGAAALVVERWLDVDVSQAKVPSVRRAMGPVSARAFGDP
                     ASAMTMLGVTGTNGKTTTTYLVEGIVRAAGFRAGVIGTTGARADGQPVPLVRTTPEAP
                     DLHRLLARMRDAGVEVVAMEVSSHALDQDRVGGVRFDAVAFTNLSQDHLDYHPSMAAY
                     FAAKASVFTPEHAVRGTVNADDPWGRRLLEEPAIPLATFAIDRDADLRATDVVVTREG
                     LEFRLGDLTIRSDLRGRFNVSNVLAAMSLARAANIPDAVAAGGVETVSEVPGRVEVVD
                     AGQRSLVVVDYAHTPDSILHVLQAARPLTTGRLIVVFGCGGDRDRAKRPLMGQAATAN
                     ADLTIITSDNPRSEDPRTIIAEIEPGAVLGGGEFRVEPDRRAAIRAAVREAGEGDTVV
                     IAGKGHEDYQELAGGTVHFDDREVAREELMAIREDA"
     gene            5111..6472
                     /gene="murF"
                     /locus_tag="VIX62_02305"
     CDS             5111..6472
                     /gene="murF"
                     /locus_tag="VIX62_02305"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY5859101.1"
                     /translation="MRPRRMSQVARAVDGLHTGADVEVSSVVTDSRAAGPGALFVALP
                     GEHTDGGLFVPEAFDNGAGGALLRDGLSAPGPAVFVRSPGDAFLQLAADERRTMSARI
                     VGITGANGKTSTKDMAAAVCARRLRTHASPESFNNEVGLPVTLLGAAADAEVVVAEMG
                     ARRKGDVTLLCEIARPDIVVVTNVGVAHLEIFGSWEAIVEASAEPVDAVGADGVAVLN
                     ADDPVVAGFAGRSAGRVVTFGTTANADVRAEDVTLDPDGLASFTLVHGQDRVAVRLAV
                     AGAHMVSNALAAAAVGLQVGVSLQEAGVALADARVSHWRMETFTTAEGIRVVNDAYNA
                     NPESVTAALKTARWMAGDGHLIAVLGTMAELGPITDEAHEHVGELAARLRVDRLITVG
                     GAASAIVTAGIREGVEPGQAAAYDDPAEALADIREHARPGDVVLFKASRVAGLEALAE
                     ALR"
     gene            6469..>7040
                     /locus_tag="VIX62_02310"
     CDS             6469..>7040
                     /locus_tag="VIX62_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY5859102.1"
                     /translation="MKSILVAAAVGLAVTLLGTPIAIRAFRVWGWGQRIREDGPHTHF
                     EKMGTPTMGGIVMLIALAVSYAATRFTSGRPTASGLALVFAAAGFGLVGFADDFTKVR
                     RRRSLGLSKSQKFLGTAVVSVLFGVIVAHYSDSSGTTTNLSFVRDTGLHLGVFFLLWC
                     FLMLTASSNAVNLTDGLDGLAAGSTTLVLSA"
BASE COUNT         1003 a         2448 c         2542 g         1047 t
ORIGIN      
        1 cagtggagga aaggggagga ggcgtggccg aactgctcgg cacgcacagc taccagctcg
       61 accccaaagg cagggtgtcg ttgccggcca ggttccgcgc cgcgttcgtc gacggggcgt
      121 ggctcaccgt tggccaggac cgatgcctct actgcttccc cagggccgaa tgggagcgac
      181 gcgccgacga ggtcgccgcc ttcccccttt ccgacaacga cggccgcgcg tactcgcgcc
      241 tgttcttcgg agcttccgag gaagtgaagc tggacgggca gggtcgcgtg acgatcccgc
      301 agcgtctgcg cgaggtcgtc gggatcgaca aagaagtcgt ggtgctcggc gttcgggacc
      361 ggatggagat ctgggaccgg gaggtctacg acgcctacgc cgccggattc gcggatgcgt
      421 accagaccgg tgcgctcgac cccaggaggt aacgacgtgg cagcaccgct cggccccaag
      481 ctcggcacgg aggttcgaca gagcctctcg ctcatcggca tgaccgcgct caccctgctc
      541 gcctcgatcg gcctgggcat cctcgcggga cacatcggct gaggtggtgg atgcgctccc
      601 gccgaacgag gacgcaccgc acgtcccggt catggtggcc gaggtgaccg ggttccttcg
      661 ggaccggggc accgtggtcg acatgacgct gggcgccggc gggcacgcgc gtgcgctcct
      721 ggaatccggc gtggagcgcg tcatcggcat cgatcgggac cggagcgcca tcaccttggc
      781 gacggagcgg ctggcgccgt acggatcgcg cttccgtgcc gtggagacga ccttctccgg
      841 cgtggaccac gagctcaccg acggcgcggt cgacgggatc ttgttcgacc tcggggtcag
      901 ctcgatgcag ctggacaccg ctgagcgggg gttcggctat cgcaaggacg ggcccttgga
      961 catgcggatg ggcggggacg gcccctccgc agccgacctg gtcaacgagc tctccgagcc
     1021 cgagctcgcg gacctgatct atgagtacgg cggcgagcat cgctcccgtc ggatcgcccg
     1081 cgccatcgtg cggaagcggc cgatcacgac gaccgatcag ctcgccggcg tcatcgcggg
     1141 tgcgctcggg aagcggccgg ggggccccca ccccgcgcgt cgcaccttcc aagcgctccg
     1201 tatcgcggtc aaccgggagc tcgatgagct cgccgcttcc ctgcctcgag cggtcgaact
     1261 cctcgctccc ggtggccgcg tggtcgtgat cgcctaccac tccctggagg accgcatcgt
     1321 caagcgatgc ttccgtgagc acgagcaggt cctgatcctc acgaagaagc cgctgcgggc
     1381 ctccgaggac gaggtggcgg ccaacccccg cgcgcgcagc gccatgttgc gcgccgccga
     1441 gcgcgtggag gtggcggcgt gagccagccg gcccgtcagc tgcccgcccc gcgcgagcct
     1501 cgtcgttcac ccaccccgtc acgtgtcgcg cctcccgcgc gcacacccgc ggcgcgcccg
     1561 cccccccccc ccccccccgg ggctcgggcg cgtcgcgggt cccaccccgc cttctggttg
     1621 ttctcggcga tcctggtggc cggcctggtc ctgggtgtcg tggccttgaa cgccctcgtg
     1681 gtgaacacca cctaccggct cgaagccgtg gagcaacgcc aacgcgcggc ggcggagtcg
     1741 ggtgcggcgc tcgaggtcca ggtggcccac cagtcctcgc cgtcccgcat ctcccggtgg
     1801 gcgggcgccc acgggatggt gctccccgat tccgatgcgg tcgtgccgct ccaggtggcg
     1861 gggccgcgcg tcggggacgc cggatgacgg ctcggggtcc gaagaaccct ccggtccacc
     1921 gcctggtggc gatgctggcc gtgatgctgt tggccctcgg ggcgatcgtc gtgcgcctgg
     1981 cgttcctcca ggtgggcgac aacggccagt tcgccgagat ggggttgcag caacgcgttc
     2041 gcacggtgga cctgcccgcg catcgcggcg agatcctcga ccggttcggc atgccgctgg
     2101 ccctcacgcg ggcggccaag gacatctacg tcaaccccac cctggtggag gaccccgagg
     2161 gcgaggccgc cacgatcgcg gacatcctgt ccatgaagca gaaggacgtc gagcaggcgc
     2221 tcgagtccga cgggaccttc gtctacctcc agcgccaggt cgacctggac atcgccgacg
     2281 aactcgaagc ccaacagctc cccgggatcg ggttcctcga ggtgccccag cggtattacc
     2341 cggcgggctc gctggcgcca caggtgctgg ggctcgtcga cgtggacggc aagggcatct
     2401 ccgggctcga gacccagtac cagacccagc tcgcgggcac gccgggggag cggaccgccg
     2461 agctgtcggc cgacggcctg ccgatctcca gcgggctcga caccctgacc gaaccggtgc
     2521 ccgggaccac cgtggtcacc acgatcgacc gccagatgca gttcatggct cagaccgccc
     2581 tcgaacgcgc ggtgcaggcc aacggcgccc tcggcggcac ggtggtcgtg atggatccgc
     2641 acaccggtga cgtctacgcc atggccacgt acccgtggtt cgacccgaac gatttctcgg
     2701 gggcctcgca ggacgcgatg cgcaaccgcg cggtcaccga ctccttcgag cccggatcgg
     2761 tcaacaagat catcaccgcg gcggccgcgc tggagacggg ctcggtgagc ctggaccaac
     2821 ggttccgggt gccgtcatcg atgcaggtgg gaccgttcac gatctatgac tcgcacgtgc
     2881 acccgatcga gacgatgacc ctgggcgaca tcatcgccga gtccagcaac atcggcgcgg
     2941 ccctcgtcgc caacgaggtc ggcaacgacc agatgggtgc ctacatgcag cgcttcgggt
     3001 acggacagcc gacggggatc gggttccccg gcgaggcctc gggcgtcctc ctgccggcat
     3061 cccagtggga cgaggtgatc cgcgccacgg tctcctacgg ccagggcatc tcggtgacgc
     3121 cgatgcagat ggccgacgtc tacgccacga tcgccaacgg cggccgctgg gtccagccgc
     3181 gactggtgcg gggcttcgaa ggtccggacg gcacgttcag cgatgcgccg gagcaacgga
     3241 cccggcgcgt cgtgagcccc accacggccg atctgctgac ccggatgctc gcctacgtgg
     3301 tggaggacgg gaccggcgcc gaagcccaga tcccgggcta ccaggtggcg ggcaagacgg
     3361 ggacctcgcg caaactcgac gacagcgggc actacgtcca gcggtacatg gcctcgttcg
     3421 tggggttcct gccggcatcg gacccgaggg tggtgatcgc cgtctcgatc gatgaacccc
     3481 gcacggtcta cggcggactg gccgccgcac ccctgttcca ggagatcgcc cggtacgcga
     3541 tccagcggct gtcgatcccg gcctcacccc cggtggccct gccgccgcac gcccaagaac
     3601 ttccatgaaa cgttgcacgg ccaggggcga gggcacgggt acgctacgac ccggtgccac
     3661 cgtccttccc ccaacgcttg ttcgctgacg tcctcggagc cgttcccgag gccgaggttc
     3721 gaggcgacgc catcatcggc gatgtggcct tcgattcccg cgacgtgacg ccgggggcgc
     3781 tcttcttctg cgtccctggg gcaacggtcg acggccacga tttcgcgatg gcggcgatcg
     3841 aggccggcgc cgccgcgctg gtcgtcgagc gatggctcga cgtggacgtg tcccaggcga
     3901 aggttccgtc ggtccgccgc gcgatgggtc cggtttcggc ccgtgcgttc ggcgaccccg
     3961 cctcggccat gaccatgctg ggggtcaccg gcacgaacgg caagacgacg acgacctacc
     4021 tggtggaggg gatcgtgcgg gcggccgggt tccgggcggg cgtgatcggt accaccggcg
     4081 cgcgcgccga cggacaaccg gtgccactgg tgcgcaccac gccggaggcc ccggacctgc
     4141 accggctcct ggcgaggatg cgcgacgccg gggtcgaggt cgtggccatg gaggtgtcgt
     4201 cgcacgccct ggatcaggac cgggtgggcg gcgtgcggtt tgacgccgtc gccttcacga
     4261 acctgtcgca ggaccacctc gactaccacc cctcgatggc ggcctacttc gcggcgaagg
     4321 cttcggtgtt cacgccggag catgcggtgc gggggacggt gaacgccgac gacccatggg
     4381 ggcgtcgcct cctcgaggag ccggccatcc cgctggcgac gttcgcgatc gatcgcgacg
     4441 ccgatctgcg cgccaccgac gtggtggtca cccgcgaggg cctcgagttc cggctcggtg
     4501 acctcacgat ccgctcggat ctgcgcgggc ggttcaacgt gtcgaacgtg ttggcggcga
     4561 tgtcgctcgc acgggcggcg aacatccccg acgcggtcgc cgccggaggc gtggagacgg
     4621 tctcagaggt gcccggccgg gtcgaggtcg tggacgccgg acagcgatcg ctggtggtag
     4681 tggactacgc acacacgccg gatagcatcc tgcacgtgct gcaggcggcg cgcccgttga
     4741 ccacgggcag gctcatcgtc gtgttcggtt gcgggggtga ccgcgaccgc gccaagcgtc
     4801 ccctgatggg gcaggcagcc accgccaacg cggatctgac gatcatcacc agcgacaatc
     4861 cgcgcagcga ggacccccgc acgatcatcg ccgagatcga accgggagcg gtcctgggtg
     4921 gcggcgagtt ccgcgtggaa cccgaccgtc gcgcggcgat ccgtgctgcg gtccgcgagg
     4981 cgggcgaggg cgacacggtg gtgatcgccg gcaagggcca cgaggactac caggaactgg
     5041 ccggtggcac ggtgcatttc gacgatcgcg aggtcgcgcg cgaggagctc atggcgatcc
     5101 gggaggatgc atgaggcccc gccggatgtc ccaggtcgcg cgcgccgtcg acggtctgca
     5161 cacgggcgcc gacgtcgagg tcagttcggt ggtgaccgac tcacgcgccg cggggccggg
     5221 ggcgctgttc gtggcgttgc ccggcgagca caccgacggc ggtctgttcg tgccggaggc
     5281 gttcgacaac ggggcggggg gagcactgct ccgcgacggt ctctccgcgc cagggccggc
     5341 ggtgttcgtg cgttcgccgg gcgatgcatt cctgcagctc gccgccgacg agcgacggac
     5401 gatgtcggcc cgcatcgtgg gcatcacggg ggccaacggc aagacctcga ccaaagacat
     5461 ggcggcggcg gtgtgcgcga ggcggttgcg gacccacgcc agtccggagt cgttcaacaa
     5521 cgaggtgggc ctgccggtga cgctgctcgg ggcggctgcc gacgccgagg tcgtggtcgc
     5581 cgagatgggg gcacggcgca aaggcgacgt cacgttgctg tgcgagatcg cgcgtcccga
     5641 catcgtcgtg gtcacgaacg tcggggtcgc acacctggag atcttcgggt cgtgggaggc
     5701 gatcgtggag gcctccgccg agccagtcga tgcggtcgga gccgacggcg tcgcggtcct
     5761 caacgccgac gatccggtgg tggccgggtt cgcgggacgg tctgcggggc gggtggtgac
     5821 cttcggcacg acggcgaacg ccgatgtgcg cgcggaggac gtcacgctcg accccgacgg
     5881 cctcgcttcg ttcacgctcg ttcatggcca ggaccgggtg gcggtccggc tcgcggtcgc
     5941 cggtgcgcac atggtctcga acgcgctcgc ggccgcggcg gtcggcctgc aggtgggcgt
     6001 gtcgttgcag gaggcggggg tcgccctggc ggacgcccgt gtgtcgcact ggcgcatgga
     6061 gaccttcacc accgccgagg ggatccgcgt cgtgaacgac gcctacaacg ccaaccccga
     6121 gtcggtgacc gccgcgctca agacggcccg gtggatggcc ggcgacgggc acctgatcgc
     6181 cgtcctcggc accatggccg aactcggccc gatcaccgac gaggcgcacg agcacgtggg
     6241 cgaactcgcg gcccggctcc gcgtcgaccg gttgatcacc gtgggcgggg ccgcgtcggc
     6301 catcgtgacg gcgggcatcc gcgaaggggt ggagcccggg caggcggccg cctacgacga
     6361 ccccgccgag gcgctcgccg acatccgcga gcacgcgcgc cccggcgacg tcgtgctgtt
     6421 caaagcctcc cgcgtcgccg gcctggaagc cctagcggag gcgctgcggt gaaatcgatc
     6481 ctcgtggccg ccgccgtcgg tctcgccgtg acgttgctgg gcacgcccat cgcgatccgc
     6541 gccttccgcg tctggggatg gggccagcgc atccgcgagg acggtccgca cacccacttc
     6601 gagaagatgg gcacgcccac gatgggcggc atcgtgatgt tgatcgccct ggcggtcagc
     6661 tacgccgcca cccggttcac gtcgggacgg cccaccgcga gcgggctcgc gctggtgttc
     6721 gccgccgccg ggttcgggct ggtggggttc gccgacgact tcacgaaggt gcgccgccgc
     6781 cggtcgctgg ggctgtcgaa gtcacagaag ttcctgggca ccgcggtcgt ctcggtgttg
     6841 ttcggcgtga tcgtggcgca ctactccgat tcgagcggaa ccaccacgaa cctgtcgttc
     6901 gtgcgcgaca ccggcctgca cctcggggtc ttcttcctgc tctggtgctt cctgatgctg
     6961 acggcgtctt cgaacgctgt gaacctcacc gacggtctcg acgggttggc ggcgggctcg
     7021 acgacgctcg tgctctcggc
//
LOCUS       DATDBZ010000096        11184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2078620, whole genome shotgun sequence.
ACCESSION   DATDBZ010000096 DATDBZ010000000
VERSION     DATDBZ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078620"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..496)
                     /locus_tag="VIX62_02315"
     CDS             complement(152..496)
                     /locus_tag="VIX62_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5859103.1"
                     /translation="MTEPAAESPICEHFQRAADLIGRRWNPQILRGLQSGVTRFSDLK
                     TGIPQISDAMLSERLKELEAEGIVTRVVTPATPVRITYGLTERGMDLSKVMDELGQWA
                     ERWADAPAATAR"
     gene            645..1208
                     /locus_tag="VIX62_02320"
     CDS             645..1208
                     /locus_tag="VIX62_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5859104.1"
                     /translation="MATEQREIDGITLPPAGKYELDASHTVIGFEARHILTKVRGRFD
                     EYSGTIVVGESPQDSSVDVEVKTGSVTSNFQQRDDHLRSADFFESDEYPVMTFHSTSI
                     RHTGGTGFELDGDLTIKDVTKPITLTGEFLGWGTDPFEKTFIAAEAKTSVDREDWDLT
                     WNMAVETGGWLVSKKIDIVIDIEAKLV"
     gene            complement(1266..1871)
                     /locus_tag="VIX62_02325"
     CDS             complement(1266..1871)
                     /locus_tag="VIX62_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HEY5859105.1"
                     /translation="MPQGTYTVLDDGPPVGTESFRSAPGPMGWRSFSEIDTAVPAPHH
                     EIVDVAVDNEWRIARVRIDTGEHDILLEPQGDVLTGFRDRQPIEIPWGPDLHLDYFTP
                     TTNAVTTKRLVTGSTEIDVVYLEPVTLEPSRVRQRYEHLGDEAVETPAGRFAATRWGF
                     TDIGTGWTSDLWVAGDVVVRYDRLFTLETYEAGASGAPLLP"
     gene            complement(1888..3042)
                     /locus_tag="VIX62_02330"
     CDS             complement(1888..3042)
                     /locus_tag="VIX62_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5859106.1"
                     /translation="MDFDLSAEQQELRAVVRAFADEVVAPAAEVHDREATFPLEVVQQ
                     MAELGFFGLTLPEAYGGSDAGTLAFAVVLEELGRADQSVAVTVSVSGSLAGGMIARLG
                     TDQQKERWLPDLAAGTALASFALTEPGGGSDAAAARTTAKLEDGEWVLEGSKAFITNS
                     GTPITSVHVVAAVTDPGQGPRGLSSILVPAGTEGLTVGAAYRKMGWHAGDTHELAFDG
                     CRVPEDHLLGERGRGFAQALSSLDGGRIAIGAIATGLAQACLDHSLAYATEREAFGKR
                     IGTYQLIQAKLADMRAKTEAARLLTHRAAWLRDQGRPHVAEAATAKLVASEAAVWCAR
                     EAVQIHGGYGYIEEFPVARFYRDAKVLEIGEGTSEILRLVIARDLGAPSA"
     gene            complement(3488..3922)
                     /locus_tag="VIX62_02335"
     CDS             complement(3488..3922)
                     /locus_tag="VIX62_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEY5859107.1"
                     /translation="MSRTIHGDLPVRFRYTPGVANTSFFEALRDRGVVLGSRCEACAV
                     TYVPARMFCERCFAELTPGIECGPGGSVESFTIGHVGIDGEALDEPVVVALVRLDRAD
                     TVLMHRLLGIEAPAIGMRVTARLRPQAERSASILDIEGFERE"
     gene            complement(3919..4500)
                     /locus_tag="VIX62_02340"
     CDS             complement(3919..4500)
                     /locus_tag="VIX62_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEY5859108.1"
                     /translation="MQVGTVVVMGTDGGTSTTATRQGTPPIGPRATPLTDEAFRSAAG
                     AVDEPLDLSYEWDTGEAMGAFLEGMRDGRVVATTCAQCERTLVPPRVFCERCFRPTDG
                     WTEVEAVGTVETFSICHVRWDMVALDPPEIPAVIRLDGASSGGFLHKLDEVAPDDVRI
                     GISVEAVWKPAEERTGSILDIAYFRPRPGGEDR"
     gene            complement(4474..5628)
                     /locus_tag="VIX62_02345"
                     /pseudo
     CDS             complement(4474..5628)
                     /locus_tag="VIX62_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014126489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
     gene            complement(5631..6698)
                     /locus_tag="VIX62_02350"
     CDS             complement(5631..6698)
                     /locus_tag="VIX62_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859109.1"
                     /translation="MEPRVGIAGVGWSGFTPTTANVSYKELMFEAARAAYLEAGVNPR
                     TDVDSFVCASEDFEEGTSIFDEYLPDQLGAVQRPVQTVASDGLFALATGVMLIRSEIA
                     SVVAVEAHSKASNVVSHGRVARFAMDPVLNRPLGVPALAVAGLEMQRYLQVSGRTAAD
                     CDAVADRNRVHAASNRRASHAEAPANAGDPLWEPLTVGQTADSVDGCVVIVLAAEDRA
                     GADPVFVQGVGWNQDAPSLESRDWDRAAATARAAEQAYARAGVTAADVDAAEVDDTYA
                     YKQLQHLDALGLSDLDPARVNRSGGALGEGYLHEGNGLARALACIEQLRAGEATTAVA
                     QSWRGVPSSSAAVAVLTRGAA"
     gene            6722..8680
                     /locus_tag="VIX62_02355"
     CDS             6722..8680
                     /locus_tag="VIX62_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5859110.1"
                     /translation="MDVVWTPTRDDIERANVTRLMRANGIETFAELRARSVADIAWFW
                     DAVVQDLGIEFTDPYLDVVDRSRGPEWATWFTQGTINLAHQCVDLWAERTPESVAVVW
                     EGEEGATRQATYAELRALTDRLANALATLGVRPRHTVGIYLPMAIETVAAIMACAKLG
                     AIWVPIFSGFGPDAVAARLADARTQVVITAGGSLRKGSPVAMKEVVDRALEHVDGVRH
                     VVVWDRLPDHPTPMTPGRDRWWHELIAGEEPWFETPALASEHPLFIGYTSGTTGRPKG
                     VVHVHGGFVVKIAEEVAYQVDLHPGEVLHWVTDLGWIMGPWEIVGALALGSTVLLTEG
                     APTYPGPDRPWATVERHGVTTLGVSPTLIRALIPHGVEPVRAHDLSSLRILASTGEPW
                     NPAPYRWFSDAIGGGRLPVINLSGGTEVGACFVSPTPVTPITGCSVGGPALGMDIDVW
                     GPDGTPVPAGEVGELVCRQPWPSMTRGVWGDDERYLDTYWRRFPGVWVHGDWARIDED
                     GAWFLLGRSDDTLSVAGKRLGPAEVESAIAAHPDVAESAAIGVPHPVKGEAIWCFVVL
                     KPGLPQSDDLERSLRDVVGEHLGKSFAPSRVVLVPDLPKTRSAKIVRRAIRAVATGED
                     PGDLSSVENPGSLEAIARALAPRPSAPG"
     gene            8690..9166
                     /locus_tag="VIX62_02360"
     CDS             8690..9166
                     /locus_tag="VIX62_02360"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEY5859111.1"
                     /translation="MTDENETQDERENRELIELLNEVRVVLPGVQVLFAFLLTLAFSS
                     AFETIDDVEKFTYLVAFFSTAGASILLMTPTAFHRIRFRQGDKEALLRMATRFILAGM
                     VLLSIAMVSGIWLVSERVMAPGAANVVGIISAVVIVVLWFGIPISRRARKKPSGAR"
     gene            9176..9991
                     /locus_tag="VIX62_02365"
     CDS             9176..9991
                     /locus_tag="VIX62_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018252029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY5859112.1"
                     /translation="MRVAICQMHSGPDVGDNLDEAEALVRAAADEGADLAALPEYLEF
                     MGPAADRRTVAQPGPGSTADRLSAVAADRGIWVLAGGILEADDAKVYDTSVMFDRVGE
                     IVARYRKIHLFDVELPDQPPIRESATITAGDQLVTTRVEEARFGLSICYDVRFPELYR
                     GLMTAGAEVLCVPSQFQFTTGKDHWHTLLRARAIENQCFVVAPGQWGPFGDPAEGRGS
                     YGHSLVVDPWGRIVIEAPEEGTGVWIADLDLAALRSLRQRFPALQHRRLGASC"
     gene            9991..10419
                     /locus_tag="VIX62_02370"
     CDS             9991..10419
                     /locus_tag="VIX62_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5859113.1"
                     /translation="MRRTLEDGYELDDDPTRVDVDAVHTYLTGSYWAMGRTRGTVERL
                     IRDATRVVGLYSNGATVGFCRVLSDDTTMAHLFDVYVLPEHRGHGLGVELVREAVERG
                     PHADLSWHLGTKDAHGLYERFGFGPPDLERQMLRRGQPRP"
     gene            complement(10457..11026)
                     /locus_tag="VIX62_02375"
     CDS             complement(10457..11026)
                     /locus_tag="VIX62_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY5859114.1"
                     /translation="MISGVVLAAGLGSRFGGTKQLAAIDGKPMAQHAVDALDGAGVDE
                     LIVVTGHDAEAVEAAIDLPSGGRFVRNTAYRDGQATSLAAAFHDIAEDSEAAVMLMGD
                     QPGITSEDVRALVDGFRATRNQIVRLRFEDGPGPALLSREIYAEAGHLHGDVGARVLI
                     ASHPEWVHEVTVPRLVPRDIDTPDDVPSD"
BASE COUNT         1656 a         3861 c         3919 g         1748 t
ORIGIN      
        1 agatctggac gtcgacccgt tccggcgccg cgtggagcag cacccggttc acgatcccga
       61 tcttggctcg cgcccattcc aggcccgtcg ggccgccatc ggggttccgg atctcccaga
      121 gttgaaaggg gtcggccatc gcgtgaggaa cctaccgggc cgtggcggcc ggtgcgtcgg
      181 cccagcgctc ggcccactga cccagctcgt ccatgacctt ggacaggtcc atgccgcgtt
      241 cggtgagccc gtaggtgatc cgcacggggg tggccggcgt gaccacccgg gtcacgatgc
      301 cctcggcttc gagttccttc aggcgctcgg acagcatcgc gtcgctgatc tgggggatcc
      361 ccgtcttcag gtcggagaag cgggtgacgc ccgactggag gccacgcagg atctgcgggt
      421 tccaccgccg gccgatcagg tcggcagcgc gctggaagtg ctcgcagatc ggcgattccg
      481 ccgcgggttc agtcatcgcg ttctccatcc ttccatcagc aaccatcgga tgcagggttt
      541 tgattcctga gcaactctgg aatagagagg gactccggtt ctgttagtca atatatgagc
      601 accgtcgcga ggggcgacgg gccgagcacc aggaggtcgg aaccatggca acggagcagc
      661 gcgagatcga cggcatcacc cttccgcccg caggcaagta cgagctcgac gccagccaca
      721 cggtgatcgg gttcgaggcc cggcacatcc tcacgaaggt gcggggtcgg ttcgacgagt
      781 acagcggcac gatcgtcgtc ggggagtcgc cccaggactc aagcgtcgac gtcgaggtca
      841 agacggggag cgtgacgtcg aacttccagc agcgcgacga ccatctgcgt agcgcggact
      901 tcttcgagtc cgacgagtac ccggtgatga ccttccacag cacgtccatc cgacacacgg
      961 gtggcaccgg gttcgaactc gatggggacc tcacgatcaa ggacgtcacc aagccgatca
     1021 ccctgaccgg tgagttcctg ggctggggca cagacccgtt cgagaagacg ttcatcgcgg
     1081 ccgaggccaa gaccagcgtc gatcgcgagg actgggacct cacctggaac atggccgtcg
     1141 agaccggcgg ctggctggtg agcaagaaga tcgacatcgt gatcgacatc gaggccaagc
     1201 tcgtctgatc gatcccgttc cggacatgcc cgtgcggaag ccggtcccga tggggccggc
     1261 ttccgtcatg gaaggagcgg tgcccccgag gcgccggcct cgtaggtctc cagggtgaac
     1321 agccggtcgt agcgcaccac cacgtcaccg gccacccaca gatcgctggt ccaccccgtc
     1381 ccgatgtcgg tgaaccccca ccgcgtcgcg gcgaaccgac ccgccggggt ctcgaccgct
     1441 tcgtcgccga ggtgttcata ccgctggcgc acgcgtgagg gttccagggt gaccggttcg
     1501 aggtagacca cgtcgatctc ggtgctgccg gtcaccagtc gcttcgtcgt gacggcgttg
     1561 gtggtgggcg tgaagtagtc gaggtgcaga tccggtcccc acggtatctc gatcggttgg
     1621 cggtcccgga agccggtgag gacgtcccct tgtggctcga gcaggatgtc gtgctcaccg
     1681 gtgtcgatgc ggacccgcgc gatacgccac tcgttgtcga ccgccacatc gacgatctca
     1741 tggtgaggag cggggacggc ggtgtcgatc tcgctgaacg atcgccagcc catcgggccc
     1801 ggcgcggatc ggaacgactc ggtgccgacg ggtgggccgt cgtcgagcac ggtgtaggtc
     1861 ccctggggca tccgtcgctc ccgcgggtca cgccgagggc gcgcccaggt ccctcgcgat
     1921 gacgagccgc aggatctcac tggtgccttc gccgatctcc agcaccttgg catcgcggta
     1981 gaagcgcgcc accgggaact cctcgatgta gccgtatccc ccgtggatct gcacggcctc
     2041 gcgcgcgcac cagacggcgg cctcgctggc gaccagcttc gcggtggcgg cctccgccac
     2101 atggggccgg ccctgatcgc ggagccacgc cgcccggtgg gtgaggagcc gcgcggcctc
     2161 ggtcttggcc cgcatgtcgg ccagcttggc ctggatcaac tggtaggtgc cgatccgctt
     2221 gccgaacgct tcgcgctcgg tggcgtaggc gaggctgtga tccaggcagg cctgcgcgag
     2281 gccggtggcg atggccccga tcgcgatccg cccgccgtcc agcgacgaca gcgcctgggc
     2341 gaagcctcgt ccgcgttcac cgagcaggtg atcctcgggg acgcggcagc cgtcgaacgc
     2401 gagctcgtgc gtgtctccgg cgtgccatcc catcttgcgg taggccgcac ccacggtgag
     2461 accctcggtg ccggccggga ccaggatcga gctgaggcca cgtggtccct gtcccgggtc
     2521 ggtgactgcc gccaccacgt gcacggaggt gatcggcgtg ccgctgttgg tgatgaacgc
     2581 cttcgaaccc tcgagcaccc actcgccgtc ctcgagtttc gccgtggtgc gagcggcggc
     2641 ggcgtcggag ccaccacccg gctcggtcag cgcgaacgag gcgagcgcgg ttcctgcggc
     2701 gaggtcgggg agccagcgct ccttctgctg gtcggtgccc aggcgcgcga tcatgccgcc
     2761 cgccaggctg cccgacaccg acacggtgac cgccaccgac tggtcggctc ggccgagttc
     2821 ttcgagcacc acggcgaagg cgagcgtgcc ggcgtccgaa cccccgtatg cctcggggag
     2881 ggtcaatccg aagaagccca gttcggccat ctgttgcacg acctcgagcg ggaacgtggc
     2941 ctcgcggtca tgcacctcgg cggccggagc aacgacctca tcggcgaacg cgcgcacgac
     3001 cgcgcggagc tcctgctgct ctgccgacag atcgaagtcc atggacggcc agcctacggc
     3061 accctcgcga ggtgggtggt cagcgcgcgt ccggccgctg ggctacggtg ccggacccgc
     3121 ggaaaagagg aggacggaga tggctgagat ccaccccggc gtgttcctgt cgcacaccga
     3181 caccgacgag tgggagttcg atcccgaggt gggcggtgac ctccacgtgc tgtgcaccga
     3241 ggtggggatc gatgccggca tgtcgcgctt cgtgggggcg acgccgtccg aaccggtggt
     3301 ctacacgcca ccgtccaggg agaccctgat cgtcctaggg agaagcacgc gtcgagatca
     3361 ccggcggtcc caccctggag ctgcgacctg gcgtgctcgc ctcgatcccc gccggtacag
     3421 aaaccacctg gcatgtgacg ccgccgttca aggagttctg ggtcatcggc tgacccgatg
     3481 ggcagcgcta ctcccgctcg aagccctcga tgtccaggat cgaggcggat cgttccgcct
     3541 gcgggcgcag ccgcgccgtc acgcgcatgc cgatcgcggg cgcctcgatg ccgagcaggc
     3601 gatgcatcag gacggtgtcg gcgcggtcga ggcgcacgag ggcgaccacg acgggttcgt
     3661 ccagcgcttc accgtcgatg cccacatgcc cgatcgtgaa ggattcgacc gaaccgccgg
     3721 gaccgcactc gatgccgggc gtgagttcgg cgaagcagcg ctcgcagaac atccgggcgg
     3781 gcacgtaggt gacggcgcac gcctcgcagc gcgagcccag caccaccccc cggtcgcgca
     3841 gcgcctcgaa gaacgacgtg ttcgcgacgc ccggcgtgta gcggaaccgc accggcaggt
     3901 cgccgtggat cgttcggctc atcggtcctc accgccgggg cggggacgga agtacgcgat
     3961 gtccaggatg gaacccgttc gttcctcggc cggcttccac acggcttcga ccgatatgcc
     4021 gatgcggacg tcgtcgggcg ccacctcgtc gagcttgtgc aggaacccac ccgaactggc
     4081 gccgtccagc cggatcacgg cggggatctc gggcggatcc agcgccacca tgtcccatcg
     4141 gacgtggcag atcgagaacg tctccaccgt gccgaccgcc tccacctcgg tccatccgtc
     4201 ggtgggccgg aagcagcgtt cgcagaacac ccgcggcggg accagggtgc gttcgcactg
     4261 cgcgcacgtg gtggcgacca cgcgcccgtc ccgcatgcct tcgaggaagg cgcccatggc
     4321 ctcgccggtg tcccattcgt aggacaggtc gagcggttcg tcgaccgcgc cggccgcgct
     4381 gcggaacgcc tcgtcggtga gcggcgtggc acgaggcccg atcggcggtg tgccctggcg
     4441 ggtggcggtc gtcgacgtgc cgccgtcggt gcccatcacg acgaccgtgc ccacctgcat
     4501 caggtcgccc catgcctgcg ccaccccgcg cttgggggtg ccggggacct ggcgcgctcc
     4561 cgcttcgcct cgcaactgcc agaagagctc ggcgatcttc atcaggccgg ccgcggcgat
     4621 cgggttgccg acgcccagca gcccgccgct ggggcacacc ggcaggtcgc cgtcgcgcgc
     4681 ggtgaccccg gcggccgccg cttcgggcgc cttgcccttg tcgaagagct gcagtccctc
     4741 caggtggtgg agttccttgt agtcgaaggg gtcgtacggc tcggcgacgt ggatctgttt
     4801 gcgtggttcg gtgacgccgg ccatgtcgta ggccatgcgg gcggcgttct ccacatactc
     4861 ggggtaggcg agatcgcggt tggtccagta ggtggagtcg aggttccagc ccacgccctg
     4921 gacccagacg ggggagtcgg tgagcttggc ggcggcctcc tcgctcgcca tcaccaacgc
     4981 gaccgcgccg tcgctgatgg gggagacgtc gagccgctgg accggatggg cgagcacctc
     5041 ggaggccagg acgtcctcca cggtgatatc ggcctggccc agcagcgcgc tggggtggtc
     5101 ggcggcgttg cgcttgttct tcacggcgac ggccgcgatg tcgcgcttgt ccaggccgta
     5161 ggcctgcatg tagcgctgca tctccagcgc gaagatccac aacaggttcg ggcccagcgg
     5221 gcgttcgatc acgttgtcga agatcgtgag gaacgcaccc tgggggtgcg gttggcagct
     5281 cgacatcttc tcttcggcca gcacgagcac gacgtcggcc atccccgagg cgatcgtgta
     5341 ccaggcctgg atcggcacga acaccccggt gccgccgccg acgaacgtgc ggatgtacgg
     5401 cttgccccag gcgccggaac cgtccgcgag gtagtcgccc ttgcggtgca cgccgtcgaa
     5461 cgcgtcgggt gccgtgcccg cgcacacggc gtcgacgtcg gcgaggctga tccccgcgtt
     5521 gtccaatgcc gcccggcccg cgatccagct gagctccttg ccggtctcct gggcgcgccg
     5581 aacgaacttc gtcatgccgg cgccgatcac cgcgaccctt cgcgccatgc tcaggcggcc
     5641 cctcgggtca ggaccgcgac ggcggcgctg ctgctgggaa ccccgcgcca ggactgcgcc
     5701 acggcggtgg tggcctcgcc cgcccgcagt tgctcgatgc aggcgagggc gcgcgcgagg
     5761 ccgttgcctt cgtgcaggta cccctcaccc agcgcgccgc ccgagcggtt gacgcgggca
     5821 ggatcgaggt ccgagaggcc gagggcgtcg aggtgctgaa gctgtttgta ggcgtaggtg
     5881 tcgtcgacct cggcggcgtc gacgtccgcg gcggtcacgc ccgcccgcgc gtacgcctgc
     5941 tcagcggcgc gcgccgtggc ggcggcccgg tcccagtcac ggctctccag cgacggtgcg
     6001 tcctggttcc acccgacgcc ttggacgaac acgggatcgg cacccgcccg gtcctcggcg
     6061 gccagcacga tcacgacgca gccgtcgacg ctgtcggcgg tctgacccac ggtgaggggc
     6121 tcccacagcg gatcgccggc gttcgccggc gcctcggcgt gggatgcgcg acggttcgag
     6181 gcagcgtgaa cccggttgcg atcggcgacg gcatcgcagt cggcggcggt gcgacccgac
     6241 acctggaggt agcgctgcat ctccaggccc gcgacggcga gcgcggggac gccgagcggg
     6301 cggttgagca ccggatccat cgcgaaccgg gccaccctgc cgtgcgagac gacgttcgag
     6361 gccttcgagt gcgcctcgac cgcgaccacc gaggcgatct cgctgcggat cagcatcacg
     6421 ccggtggcca gggcgaacaa tccgtcggag gccaccgtct ggactggccg ctgcacggcg
     6481 ccgagctggt cggggaggta ttcgtcgaag atgctggtgc cctcttcgaa gtcctcggag
     6541 gcgcagacga acgaatcgac gtcggtcctc gggttcacgc cggcttccag gtaggcggcg
     6601 cgggcggctt cgaacatcag ctccttgtag gagacgttcg ccgtggtcgg ggtgaacccc
     6661 gaccagccga cgcctgcgat gcctacccgc ggttccacgg acacggttat accgtgaagc
     6721 cgtggacgtc gtatggaccc ccacccggga cgacatcgag cgcgcgaacg tcacgcggct
     6781 gatgcgcgcg aacggcatcg agacgttcgc tgagctccgc gcccgctcgg tggccgacat
     6841 cgcctggttc tgggacgccg tggtccagga cctcgggatc gagttcaccg atccatacct
     6901 cgacgtcgtc gatcgttcgc ggggacctga atgggcgacc tggttcacgc aggggacgat
     6961 caacctcgcg caccagtgcg tcgacctgtg ggcggaacgc acccccgagt cggtcgccgt
     7021 cgtctgggaa ggggaggaag gtgcgacgcg ccaggcgacg tacgcggagc ttcgcgccct
     7081 caccgatcgg ctcgcgaacg cgctcgccac cctgggggta cgtccgcgcc acaccgtggg
     7141 catctacctg ccgatggcga tcgagaccgt cgccgcgatc atggcctgcg cgaagctcgg
     7201 cgcgatctgg gtgccgatct tcagcggctt cggccccgat gcggtggcgg cccggctcgc
     7261 cgacgcccgc acccaggtgg tgatcactgc cggcggatcc ctgcggaagg ggtcgcccgt
     7321 cgcgatgaag gaggtggtcg accgtgcgct cgaacacgtg gacggtgtgc gccacgtcgt
     7381 cgtgtgggac cgccttccgg accatccgac gccgatgacg cccggccgcg accgctggtg
     7441 gcacgagctg atcgccgggg aggagccctg gttcgagaca ccggcgctcg catcggagca
     7501 tcccttgttc atcggctaca ccagcgggac gacgggccgg ccaaagggtg tcgtccacgt
     7561 ccacggcggg ttcgtggtga agatcgccga ggaggtcgcc tatcaggtgg acctgcatcc
     7621 cggcgaggtg ctgcattggg tcaccgacct cggctggatc atggggccgt gggagatcgt
     7681 cggcgcgctc gccctgggtt cgaccgtgct gctcacggaa ggcgcgccca cgtaccccgg
     7741 gccggaccgg ccgtgggcca cggtggaacg ccacggggtg accacgctgg gggtctcgcc
     7801 aacgctgatc cgtgcgctga tcccccacgg cgtcgaaccc gtgcgggcgc acgacctctc
     7861 ttcgctccgg atcctcgcct cgaccggaga accgtggaac cccgcgccct accgctggtt
     7921 ctcggatgcg atcggcggcg gccgcctccc ggtcatcaac ctgtcgggtg ggaccgaggt
     7981 gggcgcgtgc ttcgtctcgc ccacgccggt gacgccgatc accggatgca gcgtgggcgg
     8041 accggcactc gggatggaca tcgacgtctg gggtccggac gggacccccg tgccggcggg
     8101 tgaggtgggc gaactcgtgt gccggcagcc gtggccgtcg atgacgcgtg gggtgtgggg
     8161 cgacgacgag cgctatctgg acacttactg gcggcgcttc cccggcgtgt gggtacacgg
     8221 cgactgggcg cgcatcgacg aggacggcgc ctggttcctg ctcgggcgct cggacgacac
     8281 gctgagcgtg gccggcaaac ggctgggtcc ggccgaggtg gagtccgcga tcgccgcaca
     8341 tcccgatgtc gccgaatcgg ccgcgatcgg ggtgccccac cccgtgaagg gagaggcgat
     8401 ctggtgcttc gtcgtgctga aacctggcct tccgcagagc gacgatctcg aacggtcgtt
     8461 gcgagacgtc gtgggtgagc acctggggaa gtcgttcgcg ccgtccaggg tggtactggt
     8521 gcccgatctg cccaagacgc ggagcgccaa gatcgtgcgt cgggccatcc gggccgttgc
     8581 caccggcgag gatcccggcg acctctcctc ggtcgaaaac cccggctccc tcgaggccat
     8641 cgcccgcgct ctggcgcctc gcccgagcgc ccctgggtaa ggtgagcccg tgaccgacga
     8701 gaacgagacc caggacgaac gcgagaaccg cgaactcatc gagctgctca acgaggtccg
     8761 ggtggtgctt cccggcgtcc aggtcctgtt cgcgttcctg ctgacgctgg ccttcagcag
     8821 cgcgttcgaa acgatcgacg acgtcgagaa gttcacgtac ctggtggcgt tcttctccac
     8881 cgccggtgcc tcgatcctgc tcatgactcc gacggcgttc caccggatcc ggttccgtca
     8941 gggcgacaag gaagcgctcc ttcggatggc gacccggttc atcctggccg gcatggtgct
     9001 gctgtccatc gcgatggtgt ccggcatctg gctggtgagc gagcgggtga tggcgcccgg
     9061 cgcggccaat gtcgtcggga tcatctcggc cgtcgtgatc gtcgtcctgt ggttcgggat
     9121 cccgatctcg cgtcgggcac gcaagaagcc gtccggcgcc cgatagcatc gacccatgag
     9181 ggtcgcgatc tgtcagatgc attccggtcc cgatgtcggg gacaacctcg acgaggccga
     9241 agcgctggtc cgcgccgccg cggacgaggg cgccgatctc gcggcgctgc ccgagtacct
     9301 cgagttcatg ggccccgccg cggatcgtcg aacggttgcg caacccggtc ctggttcgac
     9361 cgcggatcga ctttctgccg tggccgccga ccggggcatc tgggtcctgg ccggcggcat
     9421 cctcgaggcc gacgacgcga aggtctacga cacgtcggtg atgttcgatc gcgtgggcga
     9481 gatcgtcgcg cgctaccgga agatccacct gttcgacgtc gagctgcccg accagccgcc
     9541 gatccgggag tcggccacca tcacggcggg ggaccaactc gtcaccaccc gcgtggagga
     9601 ggcccgcttc gggttgtcga tctgctacga cgtgcggttc cccgagctgt accgcgggct
     9661 catgaccgcg ggcgccgagg tgctctgcgt gccctcgcag ttccagttca cgacgggcaa
     9721 ggaccactgg cacacgctgc tgcgagcgcg cgcgatcgag aaccagtgct tcgtcgtggc
     9781 acccggccag tgggggccgt tcggcgatcc cgccgagggg aggggttcct acggtcactc
     9841 cttggtcgtt gacccgtggg gccggatcgt gatcgaagcg cccgaggagg gcaccggggt
     9901 gtggatcgcc gacctggacc tggcggcgct gcggtcgctc cggcagcggt tccccgcgct
     9961 gcaacatcgg cggctgggag ccagctgctg atgcgccgga cgctcgagga cggctacgag
    10021 ctcgacgacg atccaacgcg cgtcgacgtc gacgccgtgc atacctatct caccgggtcg
    10081 tactgggcca tgggacggac ccgtggaacc gtcgagcggc tgatccggga cgccacgcgg
    10141 gtggtgggcc tgtactcgaa cggggccacc gtcgggttct gtcgcgtcct cagcgacgac
    10201 accacgatgg cgcacctgtt cgacgtctac gtcctgcccg agcaccgcgg gcacggactc
    10261 ggcgtcgagc tcgtgcgtga agccgtcgag cgtggtccgc acgccgatct ctcgtggcac
    10321 ctgggaacga aggatgccca cggcctctac gagcggttcg ggttcggccc gcccgacctc
    10381 gaacgccaga tgctgcgccg gggtcagccc cggccctgag gcatcgggtg ccggccgcgc
    10441 ccggctcggg tggcgctcag tccgagggca cgtcgtccgg ggtgtcgatg tcgcgaggaa
    10501 ccaacctcgg caccgtcacc tcatggaccc actcggggtg gctcgcgatc aataccctcg
    10561 cgccgacgtc accatggagg tggccggctt cggcgtagat ctcgcgtgaa aggagcgcag
    10621 gccccggccc gtcttcgaac cggaggcgca cgatctggtt ccgcgtggcc cggaacccat
    10681 ccaccagcgc gcgaacgtcc tccgaggtga tgccgggctg atcgcccatc agcatcacgg
    10741 cggcctcgct gtcctcggcg atgtcgtgga acgccgccgc caacgaggtg gcctgaccgt
    10801 cgcgataggc ggtgttccgc acgaaccgtc caccgctcgg aagatcgatc gccgcctcga
    10861 cggcctcggc gtcgtggccc gtgaccacga tgagctcgtc gaccccggct ccgtccagcg
    10921 cgtccacggc gtgctgggcc atgggcttgc catcgatcgc cgcgagttgc ttcgtccccc
    10981 cgaaccgcga ccccaacccg gccgccagta cgacccccga gatcacgacg ccttcgaccc
    11041 gcccccgaac agtcgccgga agaagttctt gatcaacgcc cacaccgccg ccaaccccaa
    11101 gccgatcccg ccaacaggct tggccacggg cttcgccacc ggcgcgggag ctgccgccgg
    11161 cggtgtggaa gcaggtgcct tggc
//
LOCUS       DATDBZ010000097         3641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1871972, whole genome shotgun sequence.
ACCESSION   DATDBZ010000097 DATDBZ010000000
VERSION     DATDBZ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871972"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VIX62_02380"
     CDS             <1..525
                     /locus_tag="VIX62_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859115.1"
                     /translation="YPAKIFMGDSGSLLLGMLLSIATISGIGRNPFPPSAGDIAAITG
                     AVLVPLLVLFIPFLDVVLAIARRTWRGQGIDHADKEHIHHRLMDIGHSHRRAVLLMYL
                     WSGLISASALAVGLIDGRTTVGLVLLAALGLFLVTALPALDRWRSRTNGSADATEAPE
                     PAAAGSGPSAEGQA"
     gene            754..1842
                     /locus_tag="VIX62_02385"
     CDS             754..1842
                     /locus_tag="VIX62_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5859116.1"
                     /translation="MFGDAMAVDDLSLDIEEGEFFSMLGPSGCGKTTTLRMIGGFEDP
                     TYGTVYLGGRDVTDLPPYKRDVNTVFQSYALFPHLDVKENVAFGLRRKKVDKSEIETR
                     VNEAMTLVDLIGFESRKPPQMSGGQQQRVALARALVNHPKVLLLDEPLGALDLKLRKQ
                     MQLELKRIQQEVGITFIYVTHDQEEAMTMSNRLAVMRLGRIEQLGDPETVYENPATEF
                     VAGFLGSANMLEGELKEGANGVATVSLRDGGSVQLPTSRVPSDVAGTVKVGVRPEKLT
                     ISRGEDATVPSGHNRVSGRVTMTTYIGVSHQYKVEAIGGTIMTAYVQNLGHEEAPRQG
                     EAVTVSWKPEHTFAVAPQEGLIMEEDEA"
     gene            1839..3134
                     /locus_tag="VIX62_02390"
     CDS             1839..3134
                     /locus_tag="VIX62_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5859117.1"
                     /translation="MTRDRKDIDPAFAASLMRGMTQRRVSRRDLIKYGGATVGTLSLA
                     SILAACGGEPAGSGGDQTSGATVDFSAPPGDAINFANWPLYIDAAKDPDTGERINPSL
                     VSFTDETGIAVNYNDEIQANASFFGELLPQLQAGQDTGRDIIVITNGREFTALTVNGW
                     VTELDPSMRPNFDANAATWAKDPDYDPGNKYSMAWQSGLTGIGYLAEKVSQPITTMDD
                     LMNPDIVGTSSVGMITGDMPDLVMINLGIDPATSGPEEWQEAANWIQMLKDSGTLRKF
                     YDQGYIDDFTAGNLAASMAWSGDVLYYKIWENKPYEYIVPEGGALLWIDNMMIPANSA
                     NPQGAYQLMDFVYQPEIAQMITEWVAYMSPVPAVQDLIAEHAAEESGEYAADLEDMAE
                     NPLLWPDDALLAKTPFGRNLTTDEEREEWDAIFVPITET"
     gene            3136..>3641
                     /locus_tag="VIX62_02395"
     CDS             3136..>3641
                     /locus_tag="VIX62_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859118.1"
                     /translation="MPQDQAKDLALVTAAAVGQSGDGPPKRADQFLAKRRSRVAYGIL
                     APGGIYLMLAFIVPLILVVYTSLQSGGLLSGGYQFNWAFENYVDAFSSSKTFIARALL
                     YSALATVLTILLAYPMAYWIAFYAGRWKSTLFFLILVPFFVSFVIRTVQWNFILADDG
                     VLFGFLKDL"
BASE COUNT          644 a         1190 c         1217 g          590 t
ORIGIN      
        1 tacccggcca agatcttcat gggcgactcc ggctcgctgc tgctcggcat gctgttgtcg
       61 atcgcgacga tcagcggcat cggccgcaac ccgttcccac cgagcgccgg cgacatcgcc
      121 gcgatcaccg gcgcggtact cgtgccgctg ctcgtgctgt tcatcccgtt cctcgacgtg
      181 gtgctcgcga tcgcgaggcg cacgtggcgt gggcagggca tcgaccacgc cgacaaggag
      241 cacatccatc accggctgat ggacatcgga cacagccacc gccgggcggt gctgctgatg
      301 tacctgtgga gcgggctgat cagcgcgagc gcgctggcgg tggggttgat cgacgggcgt
      361 acgaccgtgg gccttgtgct gctcgcagcc ctcgggctgt tcctcgtcac ggcgctcccg
      421 gccctcgatc ggtggaggag ccggaccaac ggctccgccg acgcgaccga ggctcccgag
      481 cccgcggcgg ccggctctgg gccgtcggcc gagggtcagg cgtgatcggc ggtccgacgc
      541 gacgaccagg acaccaagtg gacgagcgag gccgcccccc cttgtccgtt ccgtacaagt
      601 ggtcgatgat gcatctccga acccgcgggt tcctgactgc gtactgacag gtgatgatgc
      661 ccgccgcctg gacgcgctgc gtccgggcga ggggccgagg agggggagcg cgtgccgaac
      721 gagatcgacg ttcgcgtgga gcgcgtcacg aaaatgttcg gtgacgcgat ggcggtggac
      781 gacctttcgt tggacatcga ggagggcgag ttcttctcga tgctggggcc ttcggggtgc
      841 ggcaagacca ccacgctccg tatgatcggc ggattcgagg atcccacgta cggcacggtc
      901 tacctcgggg ggcgcgacgt gaccgacttg cccccgtaca agcgcgatgt gaacaccgtg
      961 ttccagtcct atgcgttgtt cccgcacctc gacgtgaagg agaacgtcgc gttcggcctt
     1021 cgtcgcaaga aggtcgacaa gtccgagatc gagactcggg tcaacgaggc gatgaccctc
     1081 gtcgacctga tcgggttcga gagccgcaag cccccccaga tgtccggtgg gcagcagcag
     1141 cgcgtcgcgt tggcccgggc gctcgtgaac caccccaaag tgctgctgct cgacgagccc
     1201 ctcggcgcac tcgacctgaa gctgcgcaag cagatgcagc tcgagctcaa gcgcatccag
     1261 caggaggtcg gcatcacgtt catctacgtg acgcacgacc aggaggaggc gatgacgatg
     1321 tcgaaccgcc tcgccgtcat gcgcctgggc aggatcgagc agctcggcga tcccgagacc
     1381 gtgtacgaga accccgccac ggagttcgtg gccgggttcc ttggctccgc gaacatgctc
     1441 gaaggcgaac tcaaggaggg cgcgaacggc gtcgccaccg tgtcgctgcg agacggcggc
     1501 tcggtgcagc tgccgaccag ccgcgtaccg tccgacgtgg cgggcacggt gaaggtcggc
     1561 gtgcgcccgg agaagctcac gatctcgcgc ggtgaggacg ccacggtgcc ctcgggccat
     1621 aaccgcgtct ctgggcgcgt gacgatgacc acctacatcg gcgtgagcca tcagtacaag
     1681 gtcgaggcca tcggcggcac gatcatgacc gcgtacgtgc agaacctcgg ccacgaggaa
     1741 gcaccccgtc agggcgaggc cgtcacggtc tcgtggaagc ccgagcacac gttcgccgtg
     1801 gcgccccagg aaggcttgat catggaggag gacgaagcat gacccgagac cgcaaggaca
     1861 tcgacccggc gttcgccgca tcgttgatgc gggggatgac gcagcggcga gtctcgcggc
     1921 gcgacctgat caagtacggc ggcgccaccg tcggcaccct gagcctggcc tcgatcctgg
     1981 cggcgtgcgg cggggaaccg gccggaagtg ggggcgacca aacgagtggg gcgaccgtgg
     2041 acttctcggc gccgccgggc gacgcgatca acttcgcgaa ctggccgctg tacatcgacg
     2101 cggccaagga tcccgacacg ggtgaacgca tcaatccatc gctggtgtcg ttcaccgacg
     2161 agacgggcat cgcggtcaac tacaacgacg agatccaggc aaacgcctcg ttcttcggtg
     2221 agctcctgcc gcagctgcag gcggggcagg acaccggacg tgacatcatc gtgatcacga
     2281 acggccgcga gttcacggcg ctcacggtga acggctgggt caccgagctc gatccgtcga
     2341 tgcgaccgaa cttcgacgcg aacgccgcga cctgggccaa ggaccccgac tacgaccccg
     2401 ggaacaagta ctcgatggcg tggcagtccg gcctcaccgg catcggctac ctcgccgaga
     2461 aggtgagtca gccgatcacc acgatggacg acctgatgaa ccccgacatc gtcgggacgt
     2521 cctcggtcgg catgatcacg ggtgacatgc ccgacctggt gatgatcaac ctcggcatcg
     2581 acccagcgac ctcaggcccc gaagagtggc aggaggcggc gaactggatc cagatgctga
     2641 aggactccgg gacactgcgg aagttctacg accaggggta catcgacgat ttcacggccg
     2701 ggaacctcgc tgcctcgatg gcgtggtcgg gcgacgtgct ctattacaag atctgggaga
     2761 acaagccgta cgagtacatc gtgcctgagg gtggggcact cttgtggatc gacaacatga
     2821 tgatcccggc gaactcggcc aacccacagg gcgcgtacca gttgatggat ttcgtctacc
     2881 agccggagat cgcccagatg atcaccgagt gggtcgcgta catgtcgccg gtgcccgccg
     2941 tgcaggacct catcgcggag cacgccgccg aggagagtgg cgagtacgcc gcggatcttg
     3001 aggacatggc ggagaaccct ctcttgtggc ccgacgacgc gctcctcgcg aagaccccgt
     3061 tcggtcgcaa cctcacgacc gacgaggaac gcgaggagtg ggacgcgatc ttcgtgccga
     3121 tcacggagac gtagggtgcc tcaggatcag gccaaggatc tcgcgctggt caccgcggcc
     3181 gccgtcggtc agtccggcga cgggccgccg aagcgtgccg accagttcct cgcgaagcgg
     3241 cggagccggg tcgcctacgg gatcctcgca ccaggcggca tctacctgat gctcgccttc
     3301 atcgtgccgc tgatcctcgt ggtctacacc tcgctgcaga gcggggggct gctctccggc
     3361 ggctatcagt tcaactgggc cttcgagaac tacgtcgacg cgttctcgtc ctcgaagacg
     3421 ttcatcgctc gagccctgct gtactcggcc ctcgcgaccg tgctcacgat cctgctcgcc
     3481 taccccatgg cctactggat cgccttctac gccgggaggt ggaagtcgac gctcttcttc
     3541 ctgatcctgg tgccgttctt cgtgtcgttc gtgatccgca ccgtgcagtg gaacttcatc
     3601 ctcgcagacg acggcgtgct cttcggcttc ctgaaggacc t
//
LOCUS       DATDBZ010000098         5847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1669990, whole genome shotgun sequence.
ACCESSION   DATDBZ010000098 DATDBZ010000000
VERSION     DATDBZ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669990"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VIX62_02400"
     CDS             <1..1090
                     /locus_tag="VIX62_02400"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5859119.1"
                     /translation="VPLEGRSSYISHVLHKLAPAFRPEDAYIGDLVSALGAIDAGITT
                     LLDWSHIQGSPAHTDAVVQALNDSGLRAVFAYGFPWWGKWEERQPSWFVRAATEHFST
                     KDQMVTLALAAPGPEFTDFEVSRDHWKLAREADARITTHVGVGSYGLDGKVQEMGEAG
                     LLGPDTTYIHCTTLNDTEIQMIVDTGGTVSLASPVEMMMGHGMPPIQKFLDRGLSPSL
                     SVDVETNVPSDMFNQMRSVLALQGAQASDGEKTPLESRDVLACATIVGAAANGLEDKV
                     GSLTPGKQADIILLRTDRMNVTPLNDPATAVVVGMDTSNVDTVLIAGRVMKRHGELLY
                     VDWPAVKRMVDDARDHVLAKSGFKLPSL"
     gene            1157..1465
                     /locus_tag="VIX62_02405"
     CDS             1157..1465
                     /locus_tag="VIX62_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859120.1"
                     /translation="MNFVQTISFTSTRMDEMQKLMDEFGEQSMASQAGDGGFVRNQVL
                     KDRDKENAYVVVAEFVSYEKAMENSGRPETDAFAKKMAELCDGPPTFGNYDLLSEETP
                     "
     gene            1501..2124
                     /locus_tag="VIX62_02410"
     CDS             1501..2124
                     /locus_tag="VIX62_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEY5859121.1"
                     /translation="MATRYVIAALRLGFALLTFAAIGVQLAHTIDAGNSVVNFVSFFT
                     IDSNILAAVLFGALGLTPTGERSHRTDVLRGAAVVYLVTTGVVYALLLSNADVQVTLP
                     WVNTVLHRVMPIVVFLDWLIVPPSSTISMREAAWWLAFPIVWTVYTMMRGAIVDWYPY
                     PFLDPRLPGPVEIAVTMAGIALFIGAVIAAVHWIGNARTDALRPAAA"
     gene            complement(2210..2524)
                     /locus_tag="VIX62_02415"
     CDS             complement(2210..2524)
                     /locus_tag="VIX62_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859122.1"
                     /translation="MSDYVLIYEGGSMPETPEAQKQAMDAWTAWFTTLGSAVKDQGNP
                     FGQEAKTVAADGAVSDGGSAATGYSILTANSVDEAATLAKDCPVLQGGASIRIHEVFN
                     VM"
     gene            complement(2631..4190)
                     /locus_tag="VIX62_02420"
     CDS             complement(2631..4190)
                     /locus_tag="VIX62_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5859123.1"
                     /translation="MKKARALTAFVALFAMMALSAPSSLVSPAAAGSAPIRLKVMTFN
                     IQYGASLSTLDAVIKAIRRADADVVGIQEPYGATKWIARQLGWYASPSMHMVSRFPIV
                     RPGGSTVPGSPGGRIPDGMAAYLLLGDGAVAAIAQTHTPWWPDGMRVMLKGGTPEEVA
                     AADHGKVAWTAPHLEATAPAIDEGLPTFFVGDLNSASHLDWTQEAVDALGWQPPYLDP
                     PGERYPFAWPTTVAMEDAGFRDSYREANPDPITDPGFTYCNDLYPACGPFDTWDRIDY
                     VDVAGPITTIRSRVVGEGGPYTDIVSRPWPTDHRAVVSTVDVTAVSPPSFVAPISEVV
                     KEGRSVRAAFHDDTATGRTIGLWPDGADPTSDAALVSATLDDATEDDTARLSTEGMTS
                     GDYQLAMIDDDDVVASSSVTVLRRHAHAWVRTGEDRYIQGEPIDVSWFGAPGNRYDWL
                     ALNRNCFDPESCPLRGWRYVNAASIGSARFGPDSEGVWPPRPGRYVVGLCLDDDYDCI
                     ATSEIFRIVEA"
     gene            complement(4195..>5847)
                     /locus_tag="VIX62_02425"
     CDS             complement(4195..>5847)
                     /locus_tag="VIX62_02425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY5859124.1"
                     /translation="WTVDASMPWASAVAIRNGRIVFVGKDDDARAFVGAGTEVINLRG
                     RMAMPGIHDGHIHPLSGGRTLTAASLNYAQLTLPQFLDRMAQFLADTVDQEPDTWLTV
                     GQWDAIAMGNLPDRHDLDNLDTARPIFVRSLDGHIALTNTRGLDIAGITDSTPDPSDG
                     EIDRDANGHATGLLYDGAIGLVSSHIPPPTVAQNTASLKAAFGVMAKQGITTYLDASS
                     GPDQLAALGAISDAGELTLRPHVALFAGPDQVEHPNALLNHLDDLRDQYARPDITIET
                     VKLFFDGVIEYPTQTAAMIDPYKVNNGTAEDPHWVPGTSNGPTYFPPEIANPGMAALD
                     AAGWQVHVHAIGDRAVRSALDAIEFARGQNGDLDNRHTLAHIEAIDSAEFKRFRQLNA
                     MANMQMQWAERDSYTMKRLKPYIGPDRWHRLYAAGGLETAGARLCGGSDWPVDPLLPF
                     RQIEMAVNRTADEIYKGGDLPLNREQSISLRSSIAMHTRNSAYQLHQAGLTGAITRGR
                     QADLVVLDRNLFDVKLTEVSTTKVMMTMVDGDIVHMKSSMVT"
BASE COUNT          877 a         1981 c         2014 g          975 t
ORIGIN      
        1 cgtcccgctc gagggacggt cgagctacat ctcgcacgtg ctgcacaagc tcgcgccggc
       61 gttccggccc gaggacgcct acatcggcga cctggtctcg gccctcggcg cgatcgacgc
      121 cggcatcacg acgctgctcg actggtcgca catccagggc tcgccggcgc acaccgacgc
      181 cgtggtgcag gcgctcaacg actccggcct gcgtgcggtg ttcgcctatg ggttcccgtg
      241 gtggggaaag tgggaggaac gccagccgag ctggttcgtg cgggccgcca ccgagcactt
      301 ctcgacgaag gatcagatgg tcacgctggc gctcgcggcg cccggtccag agttcaccga
      361 cttcgaggtc tcgcgcgacc actggaagct ggcgcgcgag gccgacgccc gcatcaccac
      421 gcacgtcggc gtgggcagct acggcctgga cggcaaggtg caggagatgg gcgaggccgg
      481 gctgcttggg cccgacacca cctacatcca ctgcaccacg ctgaacgaca ccgagatcca
      541 gatgatcgtc gacacgggcg gcacggtctc cctcgcctcg ccggtggaga tgatgatggg
      601 ccacggcatg ccgccgatcc agaagttcct cgatcgcggc ctgagcccca gcctcagcgt
      661 cgacgtcgag acgaacgtgc cgagcgacat gttcaaccag atgcggtcgg tgctcgcgct
      721 gcagggcgcc caggcttcgg acggggagaa gaccccgctg gagtcccggg acgtgctcgc
      781 ctgcgcgacg atcgtcggcg cggcggcgaa cggactcgag gacaaggtgg gctcgctcac
      841 gcccggcaag caggccgaca tcatcctgct gcgcaccgat cgcatgaacg tgacgccgct
      901 caacgatccg gccacggcgg tggtggtggg catggacacc agcaacgtcg acacggtatt
      961 gatcgccggc cgcgtgatga agcgccacgg cgagttgttg tacgtcgact ggcccgccgt
     1021 gaagcggatg gtcgacgatg cccgcgatca cgtcctggcg aagtccgggt tcaagctccc
     1081 cagcctctga gaccggcctt gaccgtcgtc ggtccccggc cgtacgttcg agcgaccgat
     1141 cggaaggagc cacgggatga acttcgtgca gaccatctcg ttcacgtcca cacgcatgga
     1201 cgagatgcag aagctgatgg acgagttcgg cgagcagagc atggcgagcc aggcgggaga
     1261 cggcgggttc gtgcggaacc aggtgctcaa ggaccgcgac aaggagaacg cctacgtggt
     1321 ggtggccgag ttcgtgtcct acgagaaggc gatggagaac tccggccgtc ccgagaccga
     1381 cgcgttcgcc aagaagatgg ccgagctctg cgacggtccg cccaccttcg gcaactacga
     1441 cctgctgagc gaagagacgc cgtagcccgg cgggagcccc tggcgggata ggctcccgcc
     1501 atggccacca ggtacgtcat cgccgcgctg cggctcggct tcgcgttgct cacgttcgct
     1561 gcgatcggtg tgcagctcgc gcacacgatc gacgcgggaa acagcgtcgt gaacttcgtc
     1621 agcttcttca cgatcgacag caacatcctg gcggccgtgc tcttcggggc gttgggcctc
     1681 acaccgacgg gtgagcgctc gcatcgcacc gacgtcctgc gcggcgcggc ggtcgtctac
     1741 ctggtcacca ccggcgtggt ctacgcgctc ctgctgtcga acgccgatgt gcaggtcacg
     1801 ctgccgtggg tcaacacggt gctgcatcgc gtgatgccga tcgtggtgtt cctcgactgg
     1861 ctgatcgtgc cgccctcgag cacgatctcg atgcgcgagg cggcctggtg gctcgccttc
     1921 ccgatcgtct ggaccgtcta cacgatgatg cgaggcgcca tcgtcgattg gtacccctac
     1981 ccgttcctcg atccgcgctt gcccggcccg gtggagatcg cggtgacgat ggccggcatc
     2041 gcgctgttca tcggggcggt gatcgccgcg gtgcattgga tcgggaacgc cagaaccgac
     2101 gccctgcgtc cggctgcggc ataggcgccg gcgccgccca aaggaaaagc ccgccgcaac
     2161 gcgcgatcgg cgaggatcgc gagccgcagc gggcttgccc gtcagggtct cacatcacgt
     2221 tgaagacttc gtggatcctg atgctcgcgc cgccctgcag caccgggcag tccttggcca
     2281 gggtggcggc ctcgtccacg gagttggccg tgaggatgct gtaccccgtt gcggcgctgc
     2341 cgccgtccga cacggcgccg tcggcggcca ccgtcttggc ctcctgcccg aacgggttcc
     2401 cctggtcctt caccgcggag cccagcgtgg tgaaccacgc cgtccaggcg tccatggcct
     2461 gcttctgtgc ctcgggggtt tcgggcatcg acccaccctc gtagatcagc acgtagtcgc
     2521 tcatcgttcc tcccttcggc gcggcactcc gtgtgcgcgc ttccacccct acgacgaacg
     2581 gagggacgtg aacttcgaca ctcgcctgca gaaggttgcc gaatccaggt tcaggcttcg
     2641 acgatgcgga agatctcgct ggtggcgatg cagtcgtagt cgtcgtccag gcacaggccc
     2701 acgacgtagc gccccggtct tggcggccac acgccttcgg aatcgggccc gaacctcgcg
     2761 ctgccgatgc tggccgcgtt cacataccgc caaccccgca ggggacagct ctcggggtcg
     2821 aagcagttgc ggttcaacgc gagccagtcg tagcggttgc cgggcgcacc gaaccagctg
     2881 acgtcgatcg gctcgccctg gatgtaccga tcctctcccg tacggaccca cgcgtgcgcg
     2941 tggcgccgca gcacggtgac gctcgaggag gcgaccacgt cgtcatcgtc gatcatcgcc
     3001 agctggtagt cgcccgacgt catgccctcg gtcgacaaac gtgctgtgtc gtcctcggtc
     3061 gcgtcgtcga gagtggccga caccaacgcg gcgtccgatg tggggtcggc gccatcgggc
     3121 cacagcccga tcgttcgccc ggtggcggtg tcgtcgtgga aggccgcccg cacggaccgg
     3181 ccctccttga cgacctcgct gatcggagcc acgaacgagg gcgggctcac cgctgtcacg
     3241 tcgacggtcg agaccacggc ccggtgatcg gtgggccacg gccggctcac gatgtcggtg
     3301 tacggcccgc cttcccccac cacccggctg cggatcgtgg tgatcgggcc ggccacgtcc
     3361 acgtagtcga tccgatccca ggtgtcgaac ggtccgcacg ccggatagag gtcgttgcag
     3421 taggtgaagc cgggatcggt gatcggatcg gggttggcct ctcggtagga gtcgcggaac
     3481 ccggcatcct ccatggccac cgtggtgggc cacgcgaacg ggtagcgctc cccaggcgga
     3541 tcgaggtacg gaggctgcca gcccaacgcg tcgaccgctt cttgcgtcca atccaggtgc
     3601 gaggcgctgt tcaggtcgcc cacgaagaac gtcggaaggc cttcgtcgat cgcgggcgcc
     3661 gtcgcctcaa ggtggggggc ggtccacgcc accttcccgt gatcggccgc cgcgacctcc
     3721 tcgggcgtgc cgcccttcag catcacgcgc atcccgtcgg gccaccacgg ggtgtgcgtc
     3781 tgcgcgatcg ccgccacggc gccgtcgccc agcagcaggt aggccgccat gccgtcaggg
     3841 atccggccgc ccggcgagcc cggcaccgtg ctccccccgg gccgcacgat cgggaaccgg
     3901 ctcaccatgt gcatgctggg cgatgcgtac cagcccagct gcctggcgat ccacttcgtg
     3961 gccccgtacg gctcctggat gccgacgacg tccgcgtcgg cgcggcggat cgccttgatc
     4021 acggcgtcca gcgtggacag cgatgcgccg tactggatgt tgaaggtcat caccttcagg
     4081 cggatcggcg ccgacccggc cgcggccggg ctcaccagcg acgaaggcgc gctcagcgcc
     4141 atcatcgcga agagcgcgac gaacgccgtg agggccctcg ccttcttcat ccgctcaggt
     4201 caccatcgat gacttcatgt ggacgatgtc gccgtccacc atggtcatca tgaccttggt
     4261 ggtggagacc tcggtgagct tcacgtcgaa caggttgcgg tcgagcacca cgaggtcggc
     4321 ttggcgtccg cgggtgatgg cgccggtgag ccccgcttgg tgcagctggt aggccgagtt
     4381 ccgcgtgtgc atcgcgatcg aggaccgcag cgagatcgac tgctcgcggt tgagcggtag
     4441 gtcgccgccc ttgtagatct catccgcggt gcggttgacc gccatctcga tctggcggaa
     4501 cggcagcagc gggtccaccg gccagtcgct cccgccgcac aggcgggcgc ccgcggtctc
     4561 gaggcccccg gcggcgtaca gccggtgcca gcggtccggg ccgatgtagg gcttgagccg
     4621 cttcatcgtg tagctgtcgc gttcggccca ctgcatctgc atgttggcca tggcgttcag
     4681 ctgacggaag cgcttgaact ccgccgagtc gatcgcctcg atgtgagcca gggtgtgccg
     4741 gttgtccagg tcgccgttct gcccgcgcgc gaactcgatc gcgtccaggg cgctgcggac
     4801 ggctcggtcg ccgatcgcgt gcacgtggac ctgccacccc gcggcgtcga gcgccgccat
     4861 cccggggttc gcgatctcgg gtgggaagta cgtggggccg ttcgatgtcc caggcaccca
     4921 gtgcgggtcc tccgccgtgc cgttgttgac cttgtagggg tcgatcatgg ccgcggtctg
     4981 cgtcgggtac tcgatcacgc cgtcgaagaa cagcttgacg gtctcgatcg tgatgtccgg
     5041 ccgggcgtac tgatcccgaa ggtcgtcaag gtggttgagc aacgcgttcg ggtgttccac
     5101 ctggtccggc ccggcgaaca acgccacgtg tgggcgcagg gtgagttcgc cggcgtcgct
     5161 gatcgcaccc agcgccgcga gttgatcggg gccggaggac gcgtcgagat aggtggtgat
     5221 gccctgcttc gccatcaccc cgaacgcagc cttcagggag gccgtgttct gtgccaccgt
     5281 gggtggcggg atgtggctcg agaccaggcc gatcgccccg tcgtacagca ggccggtggc
     5341 gtgaccgttc gcgtcgcggt cgatctcacc gtcgctcggg tcgggcgtgc tgtcggtgat
     5401 gccggcgatg tcgaggccgc gcgtgttcgt cagcgcgatg tggccgtcca ggctccgcac
     5461 gaagatcggc cgcgcggtgt cgaggttgtc caggtcgtgc cgatcgggga ggttccccat
     5521 cgcgatggcg tcccactggc ccacggtgag ccaggtgtcg ggctcttggt cgacggtgtc
     5581 ggccaggaac tgggccatcc gatcgaggaa ctgcggcaac gtgagctgcg cgtagttcag
     5641 cgaggcggcc gtcaaggtgc gaccgcccga cagcgggtgg atgtgcccgt cgtggatccc
     5701 tggcatcgcc atccgtccgc gaaggttgat gacctccgtg ccggcgccga cgaacgcgcg
     5761 ggcatcgtcg tccttgccca cgaagacgat ccggccgttg cggatcgcca ccgccgaggc
     5821 ccatggcatc gacgcgtcga cggtcca
//
LOCUS       DATDBZ010000099         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2693926, whole genome shotgun sequence.
ACCESSION   DATDBZ010000099 DATDBZ010000000
VERSION     DATDBZ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2693926"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="VIX62_02430"
     CDS             <1..1597
                     /locus_tag="VIX62_02430"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY5859125.1"
                     /translation="LVGLAWTSGPLTGASARVRRGGAWSEWFALDCDPSEGPDPEEAP
                     RSERRTSRPMWIDRADAVEVVVPDGAMDVDLVRVRETTRTALSITESSAEAVPGILPR
                     SAWNARSYVGTPSVTTDVRLAVIHHSVNTNGYASADVPSMIRGIQNYHIDGNGWNDIA
                     YNFVVDRFGRLWEGRAGGVDQGVIGGHTYGCNTGTIGVCYLGDLRGVAMPPAAVQAIT
                     GLLSWKFNQVHAISPGGQNYFAPVTANSTSKWEAGVVDLHPTVVGHEHFSFTECPGAH
                     SVPGVVHGTLTAFGDVRGISTAAGGAWNGMWLVNGSGQARPHAWTPFYGSMAGAALAA
                     SMVSLTPTKSGNGYWMLAADGGIFSFGDATFHGSTGAMTLNKPVVGMARTPSGNGYWL
                     VAADGGVFCFGDAKFFGSTGGLRLNKPVIGMAATASGRGYWLFASDGGIFAFGDAAFL
                     GSGGGSVLPSAAAGVAVRPDGRGYWLAFANGTVWAFGTAPQLGNASQPSSDPIVGIAS
                     TIDGGGYWLAARSGKVFSFGNAT"
     gene            1617..2249
                     /locus_tag="VIX62_02435"
     CDS             1617..2249
                     /locus_tag="VIX62_02435"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY5859126.1"
                     /translation="MFAAIVLGAAALGLLVSLVVPSHTADAWSGPLVMATAVLVASGA
                     AKVLAPTAASDALGALGAPAGLPLVRTLGVGEVILGVAAVLAGGPVLAAGVSFTYLAF
                     AVVAWRMSRSSDVRSCGCFGASGARPGLTHVVVDVATALLAGGAAAAGSGGAIALVSD
                     SPAWGIPALAGSALGAAAAIALLTVAADTAAAVDGPRPAPTTFHLVDESR"
BASE COUNT          301 a          763 c          808 g          394 t
ORIGIN      
        1 tctcgtcggc ctggcgtgga cgagcggtcc cctgaccggg gcctcggctc gcgtgcgtcg
       61 aggcggtgcc tggtcggagt ggttcgcact cgattgcgat ccgagcgagg gacctgatcc
      121 ggaggaggca ccacgatcgg agcgtcggac gagccggccg atgtggatcg accgggctga
      181 cgcggtcgag gtggtggttc ccgacggggc gatggatgtg gacctggtcc gcgtccgtga
      241 gacgacacga acggcgctgt cgatcaccga gtcgagcgcg gaggccgtgc ccggcatcct
      301 tccgcggtcg gcatggaacg ccaggtccta tgtcgggacg ccgtcggtga ccacagacgt
      361 gcgtctggcc gtgatccacc acagcgtcaa caccaacggc tacgcgtcag ccgacgttcc
      421 gtcgatgatc cgcgggatcc agaactacca catcgacgga aacggctgga acgacatcgc
      481 gtacaacttc gtcgtcgatc gcttcggtcg gctgtgggaa ggcagggcgg gaggcgtcga
      541 ccagggcgtg atcggtggcc acacctacgg atgcaacacc ggcacgatcg gtgtctgcta
      601 cctgggcgac ctgcggggcg tggcgatgcc ccccgcggcc gtgcaagcca tcacggggct
      661 gctctcgtgg aagttcaacc aggtgcacgc catctctcct ggcggccaga actacttcgc
      721 accggtcacc gcgaactcca cgagcaagtg ggaagccggc gtcgtggatc tgcatccgac
      781 agtcgtgggc cacgagcact tcagcttcac cgagtgcccg ggcgctcact cggtcccagg
      841 tgtcgtgcac ggcacgctca ccgcgttcgg cgatgtccgc ggcatcagca cggccgccgg
      901 tggtgcctgg aacgggatgt ggttggtcaa tgggtcgggg caggcccggc cccacgcctg
      961 gacgccgttc tacggatcga tggctggagc ggccctcgcg gcatcgatgg tctcgctcac
     1021 gccgaccaag tcaggcaacg gctattggat gctggcggcc gacggcggga tcttcagctt
     1081 cggcgacgcc accttccacg gctcgaccgg cgcgatgacg ctcaacaagc cggtggtggg
     1141 catggcacgc acgccatcgg gcaatgggta ctggctcgtc gcggccgatg gtggggtgtt
     1201 ctgcttcggc gatgcgaagt tcttcggctc caccggaggg ctccgcctga acaagcccgt
     1261 gatcggcatg gccgccaccg ccagtggtcg gggatactgg ctcttcgcgt ccgacggcgg
     1321 catcttcgcc ttcggcgacg cggccttcct gggttcgggt ggtggctcgg tcctgcccag
     1381 cgccgcggcc ggcgtggcgg tgcgtcccga tggccgtggc tactggctgg cgttcgcgaa
     1441 cggtacggtg tgggcgttcg gaacagcccc ccagctcggc aacgcgtcgc aaccatcgtc
     1501 ggatcccatc gtcgggatcg cctcgacgat cgacggtggg ggatactggt tggcagcgcg
     1561 gtcggggaag gtcttcagct tcggaaatgc aacctgacgc cgacagaacc ccttgggtgt
     1621 tcgccgcgat cgtgttgggt gccgccgcgc tcggtctgct cgtgagcctc gtggtgccga
     1681 gccacaccgc cgacgcgtgg tccgggcccc tcgtgatggc gacggccgtg ctcgtcgcct
     1741 ctggcgcagc caaggtcctt gctcccacgg ctgcgagcga cgctctgggg gccctcggcg
     1801 cacccgcggg cctcccgttg gtacgcaccc tcggtgtcgg tgaggtgatc ctcggcgtcg
     1861 ctgcggtgct cgccggtggg ccggtcctgg cggcaggggt gtcgttcacc tacctcgcgt
     1921 tcgcagtcgt ggcctggcgc atgtcccgat cgagcgacgt acggtcctgt ggctgcttcg
     1981 gagcctccgg cgcgcgccct gggctcaccc atgtggtcgt cgatgtcgcg acagcgctcc
     2041 ttgccggagg ggccgctgcc gccgggtccg gtggcgcgat cgctctggtc tcggactcac
     2101 ctgcctgggg catcccggcc ctcgcgggat cggccctcgg cgctgctgcc gccatcgcgc
     2161 tgctgacggt cgccgccgac acggcagcgg ccgtcgacgg cccccggcct gctcccacga
     2221 ccttccatct ggtagacgag tcgcgatgag tcgcacgtcg ttcgcc
//
LOCUS       DATDBZ010000100         4217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_440995, whole genome shotgun sequence.
ACCESSION   DATDBZ010000100 DATDBZ010000000
VERSION     DATDBZ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440995"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VIX62_02440"
     CDS             <1..990
                     /locus_tag="VIX62_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY5859127.1"
                     /translation="VAAGPPLGLYLSTETHVVSDRAADTLGLGAANVRHIAVDDGYRM
                     RVDELRAQIARDREDGIHPIAVVATAGTVSTGAIDPLDELAQVCADEGLWLHVDAAYG
                     GPAMLADDLRPQLAGIERADSVAFDPHKWLYTPHSGGCLLVRDMEVLRESFDAYAAYT
                     VQDKDATGHGIDLGRYGPSFSRGFWSLRVWVSLLAHGRDAYARRISHDAALARYLGAR
                     VEERDDFELCAPVGLSIACFRFVPPGLPADLDADAREAYLSALNQRIMTGIQLDGRVF
                     CSNAILEGRFTLRVCIVNFRTEADEMDLVLDVAAELGTQLDREMRPSLVDRPA"
     gene            987..1721
                     /locus_tag="VIX62_02445"
     CDS             987..1721
                     /locus_tag="VIX62_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEY5859128.1"
                     /translation="MSPSYALLGSGEFDPWTESVDRWILGRRGAAAGPVLILPTAAAA
                     EGDEVFDMWADKGLDHYGRLGIVACVVPLKTRQDAVRDDLIAMLDGAAAAFFSGGNPA
                     FLASVLLDTPFWSALRSAMDDGLAYAGCSAGVACLGDTALDSAREQFDDELWKPGLGV
                     FPGTWFGPHWDALDGFAPGFTDFIQASVPEGNRLVGIDENTAMAGDGRSWSVHGVGGV
                     HVLEAGAWTSHPPGSAFELSLITEGG"
     gene            1726..2349
                     /locus_tag="VIX62_02450"
     CDS             1726..2349
                     /locus_tag="VIX62_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5859129.1"
                     /translation="MSTDAFFHARSREFQDRFDTRRLADRIEEVLLHDRFDANDRAFV
                     ESRDMFFLATADEQGRPQVSYKGGDVGFVKVIDDSTLAFPNYNGNGMYLSMGNIAANP
                     HVGMLFIDFERRHRMRVNGEASIRLEDPLVEAWPEAQFVVRVRAREIFPNCPRYIHRM
                     QKVETSAFVPREDCVTPVPEWKRNDWAVDVLPADDPARDPNAEVRGA"
     gene            2359..3279
                     /locus_tag="VIX62_02455"
     CDS             2359..3279
                     /locus_tag="VIX62_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY5859130.1"
                     /translation="MPEVTVAAVQATPVFLDRDATVEVVIGRTKDAAKLGAELIVFPE
                     TFIPTYPDWVWRSPAWDGGFDGLMDRLVDQSVAVPGPATVAIGKAAKAAKAYVSVGVN
                     ELGDSGTLYNAQLMFAPDGSLIGKHRKLMPTGGERLVWGMGDGSDLEVYQTPFGRVGG
                     LICWENFMPLARTAMYAKGVEIWTAPTWDNGENWVATLRHIAREGRVFVIGVTTLLRG
                     SDVPDDVPGRELWGGEEDWCNDGWSAIVDPDGQLLAGPLVGEEGILTATIDTARLRSE
                     RYKFDPVGHYARPDVFTLTVNEAPKPTVTD"
     gene            3310..3972
                     /gene="nth"
                     /locus_tag="VIX62_02460"
     CDS             3310..3972
                     /gene="nth"
                     /locus_tag="VIX62_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY5859131.1"
                     /translation="MATKPALTRKSPIEDRAPAIIRILSKAYPDAHVALNFTNPLECL
                     IATILSAQCTDAKVNEVTATLFVKYRSAADYLAVPEDELKADIKPTGFFNQKATSIRA
                     ACDRIVTVYGGKVPDTMEDLQTLRGVARKTANIVMGNAFGKVEGIAVDTHVRRLANRL
                     GFSEESDPDKIEQDLMRLVPRKRWFDFSYVLIDHGRAICQAKKPKCAECPVSDLCPSS
                     WA"
     gene            complement(3993..>4217)
                     /locus_tag="VIX62_02465"
     CDS             complement(3993..>4217)
                     /locus_tag="VIX62_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY5859132.1"
                     /translation="TRRAIAVCHECPVRADCLEWSLDSCQDAGVWGGMGEEDRREVRR
                     QRRRDAATAGAVGGGSAPADDDAGELVSVG"
BASE COUNT          647 a         1402 c         1516 g          652 t
ORIGIN      
        1 gtcgccgcgg gcccacccct cgggctctac ctgtccaccg agacgcacgt ggtgagcgac
       61 cgcgcggccg acaccttggg cctgggtgcc gcgaacgtcc ggcacatcgc ggtcgacgac
      121 ggctatcgga tgcgggtgga cgagctccgc gcacagatcg cgcgcgaccg cgaggacggc
      181 atccacccga tcgccgtggt ggccaccgcg ggcacggtgt ccaccggcgc gatcgacccc
      241 ttggacgaac tcgcgcaggt ttgcgccgac gaaggactgt ggctccacgt cgacgccgcc
      301 tacggcgggc cggcgatgct cgccgacgac ctgcgcccgc agctggccgg gatcgaacgc
      361 gcggattcgg tggccttcga tccgcacaag tggctctaca cgccgcactc ggggggatgt
      421 ctgctggtgc gcgacatgga ggtgctccgc gagagcttcg acgcctacgc cgcctacacg
      481 gtccaggaca aggacgcaac cggccacggc atcgacctcg gccgctacgg ccccagcttc
      541 agccgcgggt tctggtcgct gcgcgtatgg gtctcgctgc tggcgcacgg gcgcgatgcc
      601 tacgcgcgac ggatctccca cgatgcggcg ctggcccgat acctgggggc acgcgtcgag
      661 gagcgggacg acttcgaact gtgcgccccg gtcggtttgt cgatcgcgtg cttccggttc
      721 gtgccgccgg ggctgcccgc cgacctggac gccgacgcac gcgaggccta cctgtcggcg
      781 ctgaaccagc ggatcatgac ggggatccag ctggacggcc gggtgttctg ctccaacgcc
      841 atcctggagg ggcggttcac gcttcgggtc tgcatcgtga acttccggac cgaggccgac
      901 gagatggatc tcgtgctcga cgtcgcggct gaattgggaa cccagctcga tcgcgagatg
      961 cggccatcgc tggtggatcg tccggcatga gtccgtccta cgccttgctc gggtccgggg
     1021 agttcgatcc ctggacggag tcggtcgatc gctggatcct gggtcgcagg ggcgccgccg
     1081 ccggccccgt gctgatcctg ccgacggccg cggccgccga gggtgacgag gtattcgaca
     1141 tgtgggcgga caagggcctc gatcactacg ggcgactcgg catcgtggca tgcgtggtgc
     1201 cgctcaagac caggcaggac gccgtccgag acgacctgat cgcgatgctc gacggcgccg
     1261 ccgcggcgtt cttctcgggc ggcaatccgg ccttcctggc ctccgtgctg ctcgacaccc
     1321 cgttctggtc ggccctgcgc tccgcgatgg atgacgggct cgcgtatgcg gggtgcagcg
     1381 ccggcgtggc atgcctcggg gacacggcgc tcgacagcgc gcgcgagcag ttcgacgacg
     1441 aactctggaa gcctggcctc ggggtcttcc caggcacgtg gttcgggccg cattgggacg
     1501 cgttggacgg gttcgcgccg gggttcacgg atttcatcca ggcctcggtg cccgagggca
     1561 accggctggt ggggatcgac gagaacacgg ccatggcggg cgacggtcgg tcgtggtcgg
     1621 tccacggggt gggcggcgtg cacgtgctgg aggccggtgc gtggacctcg catccgccgg
     1681 gctcggcgtt cgagctgtcg ctgatcacgg aaggagggtg agcctgtgtc cacggacgcg
     1741 ttcttccacg cacggagccg ggagttccag gatcggttcg acacccggcg cctggccgat
     1801 cgcatcgagg aggtcctgct gcacgatcgg ttcgacgcga acgatcgggc gttcgtcgag
     1861 tcgcgcgaca tgttcttcct ggccacggcc gacgaacagg gccgtcccca ggtgtcgtac
     1921 aagggcggtg acgtggggtt cgtcaaggtg atcgacgatt ccacgctggc gttcccgaac
     1981 tacaacggca acggcatgta cctgtccatg ggcaacatcg ccgccaaccc gcacgtcggc
     2041 atgctcttca tcgacttcga acgccgtcac cggatgcggg tgaacggcga ggcgtcgatc
     2101 cgcctggagg atccgctggt cgaggcctgg cccgaggcgc agttcgtcgt tcgggtgcgg
     2161 gcgcgcgaga tcttccccaa ctgcccgcgc tacatccacc gcatgcagaa ggtggaaacc
     2221 tccgcgttcg ttccacgaga agactgcgtg acgccggttc cggagtggaa gcggaacgac
     2281 tgggcggtcg acgtcctgcc cgccgacgac ccggcccgcg acccgaacgc cgaggtccgt
     2341 ggcgcatgag gaggatcgat gcctgaggtg accgtggcgg cggtgcaggc cacgccggtg
     2401 ttcctggacc gcgacgccac cgtcgaggtg gtgatcggcc ggacgaagga cgcggcgaag
     2461 ctgggggccg agctgatcgt gttcccagag acgttcatcc ctacctaccc ggactgggtc
     2521 tggcgctcgc cggcctggga cggtgggttc gacgggttga tggaccggct cgtggaccag
     2581 tccgtcgcgg tgcccggccc cgccacggtg gcgatcggca aggcggcgaa ggcggcgaag
     2641 gcctacgtca gcgtcggcgt gaacgaactc ggggacagcg gcacgctcta caacgcccag
     2701 ctgatgttcg cgcccgacgg atcgctgatc ggcaagcacc ggaagctgat gcccaccggc
     2761 ggcgagcgcc tggtgtgggg gatgggcgac ggttcggacc tcgaggtgta ccagacgccg
     2821 ttcggccggg tgggtgggct gatctgttgg gagaacttca tgccgctggc gcggaccgcg
     2881 atgtacgcca agggcgtgga gatctggacg gctcccacct gggacaacgg cgagaactgg
     2941 gtggccacct tgcgacacat cgcccgcgag ggacgggtgt tcgtgatcgg cgtgacgacc
     3001 ttgctgcgag ggtccgacgt ccccgacgac gtgccagggc gcgagctgtg gggcggcgag
     3061 gaggactggt gcaacgacgg gtggtcggcg atcgtcgatc ccgatggcca gctgctggcc
     3121 ggccccctcg tgggggagga aggcatcctc accgcgacga tcgataccgc gcggctgcgc
     3181 tcggagcgct acaagttcga tccggtcggc cactacgcgc ggcccgacgt cttcacgctc
     3241 acggtgaacg aggcaccgaa gcccaccgtc accgactgag cgagcggccc gcttcggtag
     3301 ggttgggcca tggccaccaa gcccgcgctc acgcgcaaga gcccgatcga agaccgagcc
     3361 cccgcgatca tccggatcct gtcgaaggcc tatcccgacg ctcacgtcgc cctcaacttc
     3421 acgaacccgc tggagtgcct gatcgcgacg atcctgtcgg cacagtgcac cgacgcgaag
     3481 gtgaacgagg tcacggcgac cctcttcgtc aagtaccgct ccgcggccga ctacctcgcg
     3541 gtgcccgagg acgaactgaa ggccgacatc aagcccacgg ggttcttcaa tcagaaggcg
     3601 acctcgatcc gcgcggcgtg cgaccgcatc gtcaccgtct acggcggcaa ggtgcccgac
     3661 acgatggagg atctgcagac cctgcgcggg gtcgcgagga agaccgccaa catcgtgatg
     3721 ggcaacgcct tcggcaaggt cgaggggatc gccgtcgaca cgcacgtgcg tcgcctggcg
     3781 aaccgccttg ggttcagcga ggagtccgac ccggacaaga tcgagcagga cctgatgcgg
     3841 ttggtccccc ggaagcggtg gttcgatttc agctacgtgc tcatcgatca cggtcgcgcg
     3901 atctgccagg cgaagaagcc gaagtgcgcc gagtgcccgg tgagcgatct gtgtccgtcg
     3961 agctgggcgt gacgcggagc cgccgtgcga cgtcagccga cgctgacgag ttcgccggcg
     4021 tcgtcatcgg ccggggcgga accgccgccc acggcgccgg ccgttgcggc atcgcgacgt
     4081 cgctggcgcc ggacctcgcg ccggtcctcc tcgcccatgc cgccccacac gccggcgtcc
     4141 tggcaggagt cgagcgacca ttccagacag tcggctcgga cggggcactc gtggcagacc
     4201 gcgatggcgc gccgggt
//
LOCUS       DATDBZ010000101         4981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2694961, whole genome shotgun sequence.
ACCESSION   DATDBZ010000101 DATDBZ010000000
VERSION     DATDBZ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694961"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VIX62_02470"
     CDS             complement(<1..427)
                     /locus_tag="VIX62_02470"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859133.1"
                     /translation="MGGWRYLGRKLLQAAVTLLFIISVNFFLFRVMPSDPVRLLTRQR
                     GVQLSPQAQQALTEELGLHLPLPTQFLRYIGDTFRGDFGNSFIYPGRSVTSVFLGDMW
                     RSLLLVGTATVLMIVIGLYLGIRGGWKRDRPLDRASMGFS"
     gene            complement(449..2257)
                     /locus_tag="VIX62_02475"
     CDS             complement(449..2257)
                     /locus_tag="VIX62_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5859134.1"
                     /translation="MRRSIRTVVVTGILVAVAVTTFAGIAGAQSDSPSPAPDVTFTIG
                     VTGDLNSANPLSQIDSTESFLTGLMYDGILRLGQADYAVEPELLTEVPTTENGGISED
                     GLTWTLHFREDAYWSDGEPVTAHDFKWTADFIMDNDISSYTDGYQPFTESIKVKDDYT
                     IVWKTKGPSLVPGLPGYSLVLPAHVWGEMDVKEIKDFKNFPDTVTSGAFRLVEWEPGE
                     FWRVEANADYNQGAPGVDEIVFRVYNSDEAVAQALIKGEIDATQIATPGLFETVKDKP
                     NIGSAVTSAEGFHQMSFNIVDDPKSTAHPAVLDPVVRQAISWAIDKPTLVDRVLRGYG
                     TPGTTPVIPLSPFWHWEPTAEEAIGFDLAEANRLLDEAGYVDTDDDGVREMPDGGDPL
                     EFRLFLATTDPAAIKASPFVEGWLRDIGIDVSTKTMTDSKLYDQWYGFDWDMIIYSWG
                     VGPDPDFILSTFTSEQCGFWSDTCYANPEYDELYEQQQVTLDETERQAIVYEMQQMIY
                     RDIPEIVLWYPNYFEAWRSDRWEGYVRWPEPDGAVFWNNPYSALSVQPKANASTTSTS
                     DSGLPAWVWVAGIAVVGAGFVGVFLARRKRSEHYAG"
     gene            2363..3742
                     /gene="selA"
                     /locus_tag="VIX62_02480"
     CDS             2363..3742
                     /gene="selA"
                     /locus_tag="VIX62_02480"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY5859135.1"
                     /translation="MADARRSVPSVDSLLRAEPVQRAAEVFGLPLVKRTVKITLDTAR
                     RHAAEGEVPPDAEEIMATAIGHASRIATGLTPVINATGVVLHTNLGRAPMPKRAADAA
                     HRAAVSYADLEVDRITGRRGTRTGRAEAMLLALTGAEAAIVVNNGAAAVLLSLAALAK
                     GKQVLVSRGELIEIGGEFRIPDIMAASGTKLVEVGTTNRTRIGDYRTAMTERGGAILK
                     VHPSNYRVVGFTAEASTKDLATLASQHDVPLMYDVGSGLLHRERGMPTDEPTVSGALA
                     DGSDLVLCSGDKLLGGPQAGLVVGKADLVAKLRKHPIARAVRVDKMQIAAMEQVLAMH
                     VRGDEQEIPVHRMLREPADVVRTRAQQLCETLGGDLEHAHVHRCESVVGGGSMPGTAL
                     ASWGVRLTVPDPTTFAARLRAGSPSVFCRVEDDHVLFDVRTVTDEQVHDTARAILYAI
                     EGDEFLDED"
     gene            3744..>4981
                     /gene="selB"
                     /locus_tag="VIX62_02485"
     CDS             3744..>4981
                     /gene="selB"
                     /locus_tag="VIX62_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEY5859136.1"
                     /translation="MTGDAGEVVTGVSDAAELRVVATAGHVDHGKSSLIVALTGIDPD
                     RWDEEKRRGLTIDLGYAWCTLPSGREIGFVDVPGHERFITNMLAGVGPVPSVLFVVAA
                     DEGWKPQSEEHLQILDVLGVRAAVVALTKADLVDAGAVATAADLVREHLAGTTLADAP
                     ILPVASTVGTGIEDIERALDQMLEVVPTPEPARTRLFVDRVFTIKGSGTVVTGTLSGG
                     PLSIGEDVALEPGDTRGRIRSLQTHEQARDRAFPVARVAANLAGIERDQVTRGDVLTQ
                     PGAWDPSAVFDALVRPVRGLAHAISSRGAHKVYAGAAEADARIRLLGPADALAPDEEA
                     FVRIRTSRPLVLDVFDRIVIRDAGRQGTVAGGIVLDVMPPRRAGTDRVRQLQARASAV
                     RDELPGLLAGERGAVRADAAA"
BASE COUNT          826 a         1686 c         1656 g          813 t
ORIGIN      
        1 aggagaaccc catggaggcg cggtccaacg gtcgatcccg tttccaacca ccgcggatcc
       61 ccaggtagag accgatcacg atcatcagga cggtggcggt gccgaccagc agcaggctgc
      121 gccacatgtc gcccaggaac accgaggtca cggaacgacc cgggtagatg aacgagttgc
      181 cgaagtcacc acggaaggtg tcgccgatgt atcggaggaa ctgcgtgggc aacggcaggt
      241 gcaaccccag ctcctcggtc agcgcctgct gagcctgcgg cgacagttgg accccacgtt
      301 ggcgtgtgag cagtcgaacc ggatcgctcg gcatcacccg gaacaggaag aagttgacgc
      361 tgatgatgaa caacagagtg acggcagcct gcagcagctt gcgtccgagg tagcgccatc
      421 ctcccatcgg tgcgggcgac cctccgatct agccggcgta gtgttcgctg cgcttgcgac
      481 gagcgaggaa gaccccgacg aagcccgcgc ccacgaccgc gatcccggcg acccagaccc
      541 acgcgggcaa tccgctatcc gacgtcgacg tcgtcgacgc gttggccttc ggttgcacag
      601 acagcgccga ataggggttg ttccagaaca cggcgccatc gggctccggc cagcgaacgt
      661 acccctccca ccgatcggat cgccacgcct cgaagtagtt ggggtaccag agcacgatct
      721 cggggatgtc tcggtagatc atctgctgca tctcgtagac gatggcctgg cgctcggtct
      781 catcgagcgt cacctgctgc tgctcataca gttcgtcgta ttcagggttg gcgtagcacg
      841 tgtcgctcca gaacccgcac tgttcgctcg tgaaggtcga caagatgaag tccgggtccg
      901 ggccgacgcc ccacgagtag atgatcatgt cccaatcgaa cccgtaccac tggtcgtaca
      961 gcttgctgtc ggtcatcgtc ttggtcgaca catcgatccc gatgtcgcgg agccagccct
     1021 cgacgaacgg cgaggccttg atcgctgccg ggtcggtggt cgcgaggaac aaccggaact
     1081 cgagcgggtc accaccgtcc ggcatctcgc ggacaccgtc atcgtcggta tcgacgtacc
     1141 cggcctcgtc gagcaagcgg ttggcctccg cgaggtcgaa cccgatcgcc tcctcagcgg
     1201 tcggttccca gtgccagaag ggcgaaagtg ggatcaccgg cgtcgtcccc ggtgttccgt
     1261 agccgcgcag cacgcgatcg acgagggtcg gcttgtcgat cgcccaggag atcgcctgcc
     1321 gcacgaccgg atccaggacc gcggggtgag ccgtgctctt ggggtcgtcg acgatgttga
     1381 acgacatctg atggaaaccc tcggcgctcg tcaccgccga tccgatgttc ggcttgtcct
     1441 tcacggtctc gaacaagccc ggggtcgcga tctgggtggc gtcgatctca cccttgatca
     1501 gcgcctgtgc gacggcctcg tccgagttgt agacacggaa gacgatctcg tcgaccccgg
     1561 gagccccctg gttgtagtcg gcattcgcct cgacccgcca gaactcgcct ggctcccact
     1621 cgacgagcct gaaggctcca ctggtgacgg tatcggggaa gttcttgaag tctttgatct
     1681 ccttgacgtc catctcgccc cacacgtgag caggcaagac gaggctgtaa ccgggtaggc
     1741 cgggcaccag gctcggcccc ttggtcttcc acacgatcgt gtagtcgtcc ttgaccttga
     1801 tgctttcggt gaacggctgg tagccgtccg tgtacgagct gatgtcgtta tccatgatga
     1861 agtcggcggt ccatttgaag tcgtgcgccg tcactggctc gccatcgctc caataggcgt
     1921 cctcgcggaa gtgcagcgtc caggtcaacc cgtcctcgga gatcccgccg ttctccgtgg
     1981 tggggacctc ggtgagcaac tcgggctcga cggcgtagtc ggcttgaccg agtcgaagga
     2041 tcccgtcgta catcagcccc gtcaggaatg actcggtgga atcgatctga ctgagggggt
     2101 tggccgagtt gagatcgccg gtcacaccga tcgtgaaggt tacgtcgggt gcggggctcg
     2161 gggaatcgga ctgggcgccg gcgatgccgg cgaacgtggt caccgccacg gccaccagga
     2221 taccggtcac cactaccgtg cggatgcttc gcctcatcga cccgaccctc cgaccatctg
     2281 taggtgcgcg catcctaggc tctcctgact cgttgatgta cccaccgaac gttgaccacc
     2341 ggtcacgcag taggcttcgg gcatggccga cgcacgccga tccgtgccct ccgttgattc
     2401 gctcttgcgg gccgaaccgg ttcagcgagc ggccgaagtc ttcggccttc cgctggtgaa
     2461 acggaccgtc aagatcaccc tcgataccgc ccgccgacac gcggccgaag gcgaggttcc
     2521 gcccgacgcc gaagagatca tggccaccgc catcggtcat gcgtcccgga tcgcgaccgg
     2581 cctcacgccc gtcatcaacg ccaccggcgt cgtgctccac acgaacctgg gtcgcgcgcc
     2641 catgccgaag cgcgccgcgg acgccgcgca tcgggcggcc gtgagctacg ccgatctcga
     2701 ggtcgaccgg atcaccggac gccgcggaac ccgcaccggc cgggccgagg cgatgctgct
     2761 ggcgctgacc ggcgccgagg ccgcgatcgt ggtcaacaac ggtgccgccg ccgtgctcct
     2821 ttcattggca gcgctggcca agggcaaaca ggtcctggtg tcgcgcggag agctgatcga
     2881 gatcgggggc gagttcagga tcccggacat catggcggcc tccgggacca aactggtcga
     2941 ggtcggcacc accaatcgca cgcgcatcgg tgactaccgc accgccatga cggaacgcgg
     3001 gggcgccatc ttgaaggtcc acccctccaa ctaccgcgtc gtcgggttca ccgccgaagc
     3061 ctccacgaag gatctcgcca cgttggcgtc ccagcacgac gtgccgctga tgtacgacgt
     3121 gggttcgggt ctgctgcacc gcgagcgcgg catgcccacc gacgagccca ccgtctcggg
     3181 tgcgctggcc gacggctccg acctcgtgct ctgttcaggc gacaagctgc tgggcggccc
     3241 gcaggccgga ctggtcgtgg gcaaggcgga cctcgtcgcc aagcttcgca agcacccgat
     3301 cgctcgagcg gtccgggtgg acaagatgca gatcgccgcg atggagcagg tgctcgcgat
     3361 gcacgtgcgg ggggacgagc aggagatccc cgtgcatcgc atgcttcgcg aacccgcaga
     3421 tgtggtgcgc acgcgcgcac agcagttgtg cgagacgctc ggcggggatc tggaacacgc
     3481 ccacgtccat cgatgtgagt cggtggtggg cggcggctcc atgcccggca ccgccctggc
     3541 ctcgtggggg gttcgcctca cggtgcccga ccccacgacg ttcgcggcgc gcttgcgagc
     3601 gggcagcccc tcggtcttct gccgggtcga agacgaccac gtgctcttcg atgtgcggac
     3661 cgtcaccgac gaacaggtgc acgacaccgc ccgagccatc ctgtacgcga tcgaaggcga
     3721 tgagttcctc gacgaggact gatgtgacgg gggacgccgg cgaggttgtc accggcgtct
     3781 ccgacgccgc cgaactccgt gtcgttgcga cggcgggcca cgtcgatcac ggcaagtcct
     3841 cgctgatcgt ggcgctcacc gggatcgacc ccgaccgctg ggacgaggag aagcggcgag
     3901 gtctcacgat cgacctgggc tacgcctggt gcaccctgcc gagcggtcgc gagatcgggt
     3961 tcgtggacgt gccgggacac gaacggttca tcaccaacat gctcgcgggg gtcgggccgg
     4021 ttccctccgt gctgttcgtg gtggccgccg acgaggggtg gaaacctcaa tccgaggagc
     4081 acctgcagat cctggatgtg ctgggggtgc gagccgccgt cgtggcgctg accaaggccg
     4141 acctggttga cgcaggtgcc gtggccacgg cagccgacct ggtccgcgaa cacctggcgg
     4201 gcaccaccct ggccgacgcg ccgatcctgc cggtggcgtc caccgtgggc accggcatcg
     4261 aagacatcga gcgggcgctc gatcagatgc tcgaggtcgt ccccacaccc gaacccgctc
     4321 ggacccggct cttcgtcgac cgcgtcttca cgatcaaggg ctcggggacg gtcgtgaccg
     4381 gcacgctctc cggggggcct ctttcgatcg gtgaggacgt cgcgttggag ccgggcgaca
     4441 cccgtggcag gatccggtcg ctgcagacgc acgagcaggc gcgcgaccgc gcgttccccg
     4501 tcgccagggt ggccgccaac ctcgccggca tcgaacgcga ccaggtcacg cggggcgatg
     4561 tcctcaccca gcccggcgcg tgggaccctt ctgcggtgtt cgacgccctg gtgcgtccgg
     4621 tgcgagggct cgcccacgcc atcagctccc gcggggccca caaggtgtat gccggcgccg
     4681 ccgaggccga cgctcggatc cgactgctcg gccccgccga cgcactggcg cccgacgaag
     4741 aggcattcgt ccggatccgc accagtcggc cgctggtcct ggacgtcttc gatcggatcg
     4801 tgatccgtga tgccggcaga cagggaaccg tcgcaggcgg gatcgtcctc gacgtgatgc
     4861 cacctcggcg agcgggcacc gaccgggtgc gccagctcca ggcacgcgca tcggcggtcc
     4921 gcgatgagct cccgggtctg ctggcgggcg aacgcggggc ggtccgcgcc gacgcggccg
     4981 c
//
LOCUS       DATDBZ010000102         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1873695, whole genome shotgun sequence.
ACCESSION   DATDBZ010000102 DATDBZ010000000
VERSION     DATDBZ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873695"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VIX62_02490"
     CDS             complement(<1..239)
                     /locus_tag="VIX62_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY5859137.1"
                     /translation="MLLVIGTRPEAIKLAPVVLVFRSLYPHVETRVALTSQHATLAIG
                     ALERFGIVPDVDLAIMSPAQSPTLVAALVLQHMEGL"
     gene            complement(371..1858)
                     /locus_tag="VIX62_02495"
     CDS             complement(371..1858)
                     /locus_tag="VIX62_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5859138.1"
                     /translation="MRQIGPELEVADRSVYARAVARFRDAGIVLPTFGQLADPGSIPA
                     DIKTRLETVDADTPHPANLFRTHWYNGRGTAYALPRPEHVVLPVELTGVEAPIVVLLG
                     DRFPMIHSHKVIAAYGCLAPRIVSGRFDPGGQRAVWPSTGNYCRGGVAISRIMGSRGV
                     AVLPEGMSRERFEWLAAWVNDPADIIRTPGTESNVKEIYDRCAELEGEPDTVVFNQFS
                     EFANHVAHYLLTGRAVDDVFGAMHLADPGLRLAGFISATGSAGTIAAGDYLKERHGSL
                     TVAVEALECPTLLRNGYGEHNIQGIGDKHVPLIHNVMATDIVVGISDRATDSLFVLFG
                     SDAGREHLRTHMGVPEALIVALPSFGISSICNILAAIKVAHKQRLGPRDVLVTVATDG
                     AEMYASEVARIVDRDHAGRFDQAAAARVSAAYLDTIDTSDVLVCGPEERERMFNLGYF
                     TWVEQRGVPLDAFEARRSQAFWAETRGAAGAWDAMIAAFNAEVAV"
     gene            1929..>2212
                     /locus_tag="VIX62_02500"
     CDS             1929..>2212
                     /locus_tag="VIX62_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY5859139.1"
                     /translation="MLITLTTTGSRSGQPRDAQLYAFEDGDDLIVTGSRGGATTDPAW
                     AHNLRAHPAAFVCRGRTRLAVRAHEADGRQRDRLWQLVCAGFPMYESYQR"
BASE COUNT          340 a          746 c          772 g          354 t
ORIGIN      
        1 aggccctcca tgtgttggag gacgagggca gccaccagcg tcggggattg agcaggcgac
       61 atgatggcga ggtcgacgtc gggcacgatg ccgaagcgtt cgagagcccc gatcgccagc
      121 gtcgcgtgct gcgaggtcag cgccacccgt gtctcgacgt gggggtagag cgaacggaac
      181 acaaggacga ccggggcgag cttgatcgcc tcaggacgtg tcccgatgac caacagcacg
      241 cgcggccgtc gttgtctcac cttcgggcac ctggctagct accaactcga tgggatcctc
      301 tcccgaaccc attccgcggt tgggccttgc ggacgagcct actcccatca ggtcccgtcg
      361 cgggcagcag tcacacagcc acctccgcgt tgaaggcggc gatcatggcg tcccacgcgc
      421 cggctgcgcc acgcgtctcc gcccagaacg cctggctgcg ccgcgcctcg aaggcgtcga
      481 gcggcacgcc tcgctgctcg acccacgtga agtagcccag gttgaacatg cgctcccgct
      541 cctcgggtcc gcacacgagc acatcggacg tgtcgatggt gtcgagatac gccgcgctta
      601 cccgagccgc ggcggcctgg tcgaaccttc cagcgtggtc gcgatccacg atgcgtgcca
      661 cctcggaggc gtacatctcc gcgccatcgg tggccacggt gacgagcacg tcgcgaggtc
      721 ccaggcgttg cttgtgggcg accttgatgg cggccaggat gttgcagatc gacgagatgc
      781 cgaaggacgg cagggccacg atgagcgcct ccggcacgcc catgtgggtg cgcaggtgct
      841 cgcgaccggc atcactgccg aagagcacga acaggctgtc ggtggctcga tcgctgatgc
      901 ccacgacgat gtccgtggcc atgacgttgt ggatgagcgg aacgtgcttg tcgccgatgc
      961 cctggatgtt gtgctcgccg tagccgttgc gcaggagcgt tgggcactcg agcgcctcca
     1021 cggccacggt gagcgatccg tggcgctcct tgaggtagtc gcccgcagcg atcgtgccgg
     1081 cggatccggt cgccgagatg aagcccgcca ggcgcagccc tggatcggcc aggtgcatgg
     1141 ccccgaacac gtcatccacg gctcgccccg tgaggaggta gtgggcgacg tggttcgcga
     1201 actcgctgaa ctggttgaag acgaccgtgt ccggctcgcc ctcgagctcg gcgcagcggt
     1261 cgtagatctc cttgacgttc gattccgtgc ccggcgttcg gatgatgtcg gcggggtcgt
     1321 tgacccacgc ggccagccac tcgaagcgct cccggctcat gccctcgggc agcacggcca
     1381 caccgcggct gcccatgatg cggctgatgg ccacgccgcc acggcagtag ttgccggtgg
     1441 agggccacac cgcgcgctgg cctccgggat cgaaccggcc gctgacgatg cgtggcgcca
     1501 ggcagccgta ggcggcgatg accttgtgcg agtggatcat cgggaagcga tcgcccagga
     1561 gcacgacgat gggcgcctcg acgccggtca gctccacagg caggacgacg tgctctggtc
     1621 gcggcagcgc gtaggccgtg cctcgaccgt tgtaccaatg ggtccggaag aggttggccg
     1681 ggtgcggcgt gtccgcgtcg acggtctcca ggcgcgtctt gatgtcggca gggatggagc
     1741 cagggtcggc gagctgcccg aacgtgggca ggacgatgcc cgcatcgcgg aagcgcgcca
     1801 ccgcacgggc gtacacgctg cggtccgcga cctccagctc cggtccgatc tgccgcatcg
     1861 ctcctccctg tcgtcgcgta tcgctcgccg atgatgctcg tgtctcgtcg ctcgggtacg
     1921 gtgagtcggt gctcatcacg ctgacgacga cggggagtcg ctcgggccag ccccgggacg
     1981 cgcagctcta cgcgttcgag gatggcgacg acctcatcgt cacgggctcc aggggcggcg
     2041 ccacgaccga cccggcctgg gcccacaacc tgcgcgctca ccctgctgcc ttcgtgtgcc
     2101 gtggccggac ccgactggca gtgcgcgccc atgaggcgga cggccgccag cgcgatcgac
     2161 tctggcagct cgtgtgtgcg gggttcccga tgtacgagtc gtaccagcgc aa
//
LOCUS       DATDBZ010000103         2990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2695477, whole genome shotgun sequence.
ACCESSION   DATDBZ010000103 DATDBZ010000000
VERSION     DATDBZ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2695477"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VIX62_02505"
     CDS             <1..275
                     /locus_tag="VIX62_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase type 12"
                     /protein_id="HEY5859140.1"
                     /translation="ENKAVAVRNIADVLSPEGVLFGGTVLGLQGNHTKPARAFLRAAN
                     KQGGFDNVDDTADGVRRILEMSFSAVDVDVVGSAAFFAAAGPRSRS"
     gene            complement(333..1079)
                     /locus_tag="VIX62_02510"
     CDS             complement(333..1079)
                     /locus_tag="VIX62_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5859141.1"
                     /translation="MNLRVVTYAERPELEDRWDETVGPVWPEFLLHDAIVNELWGQLF
                     EIAPEHQFYVVDGDTDSVVCVGNSLPFRWDRNPASLPDGIDGVLPLAIEQYENGIEPN
                     TLCALQAVVVPAYQGQGHAAVPIRQMADAARRAGFADLVAPVRPSWKQRFPLIPMQEY
                     ASWTRSDGLPFDPWFRVHARMGASFLQVCHNSLRIVGTVDEWRAWTALEFPGSGSYPI
                     PGALVPIEIDVARDEGVYIEPNRWMHHRIR"
     gene            complement(1076..1924)
                     /locus_tag="VIX62_02515"
     CDS             complement(1076..1924)
                     /locus_tag="VIX62_02515"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY5859142.1"
                     /translation="MDAAVPGTGPWSWILTWPADPFALAVLMAVGFVYAGGLRRLRLA
                     GTPFPRRWAFAFWCGIGALGLALISPVDAYADASFNVHMAQHLLLTFVAPPLLALGAP
                     ITLALRACSAPRARSITGILRGRIGRTLSNPVVGFTLFVGVPFAIHMSPLFDLALRAT
                     FVHRAEHAAWIAAALIYWWPIVGRDPTPHPMPHPARMLSLFIAMPMMSFLALSIYIAD
                     EPLYPTYAALPAPWGTQALVEQRWAAVQMWLVGNFALVLAILLVAVAWKHAEDESQRR
                     LEIRLR"
     gene            2030..2566
                     /locus_tag="VIX62_02520"
     CDS             2030..2566
                     /locus_tag="VIX62_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEY5859143.1"
                     /translation="MSPVRFGTILFLASELLLFGGLFAAYFGLRARTDPWPPGDIEPD
                     WQLALFGTVLLVVSSFCLSRGLDHAAKGRVDLLRRWTLVTMVLGVGFLAMEVWDWTHV
                     SFEISSSPYGTMYFTLTGIHGLHVLAGLLVLSVLMGRSFQGAYDTGNLDGAHSMAYYW
                     QFCDATWILIFLVVFVLS"
     gene            2563..>2990
                     /locus_tag="VIX62_02525"
     CDS             2563..>2990
                     /locus_tag="VIX62_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5859144.1"
                     /translation="MTRRSAEARLLSRVLRIGLLLAVGAQIVWSFAPSRGEASSASSQ
                     VGQTLFEANCSTCHGLNAEGTANGPSLQGVGPAAVDFMLTTGRMPLAAPEDQPDRQPP
                     KFSPDQIDEIVAYVAEIAPGGPAIPDVDTARGDLPTGAAL"
BASE COUNT          489 a          983 c         1018 g          500 t
ORIGIN      
        1 aggagaacaa ggcggtcgcc gtgaggaaca tcgcggacgt cctcagcccg gaaggggtcc
       61 tgttcggcgg gacggtcctc ggactccagg gcaaccacac gaagcccgcg cgcgcattcc
      121 tgcgagcagc caacaagcaa ggaggcttcg acaacgtgga cgacactgcg gatggggtcc
      181 gccgcatcct ggagatgtcc ttcagcgccg tggatgtcga cgtcgtcggt tcggcggcct
      241 tcttcgccgc ggccgggcca cggtcccgct cctagacgct gccaggtcgg agcgggcatc
      301 ctcccgaacc ccgcttgcct ggggtgtcgg cctcatcgga tccggtggtg catccatcgg
      361 ttcggctcga tgtagacgcc ttcgtcgcgt gcgacatcga tctcgatcgg gacgagggcg
      421 ccggggatcg ggtagctgcc cgagcccggg aactcgagcg ctgtccacgc ccgccactcg
      481 tcgacggtgc cgacgatccg cagcgagttg tggcacacct gaaggaacga cgctcccatg
      541 cgagcgtgga cgcggaacca gggatcgaag ggcagaccat cggaccgtgt ccatgacgcg
      601 tactcctgca tcgggatcag tgggaagcgc tgtttccagg agggacggac aggtgcgacg
      661 agatcggcga acccagcgcg gcgcgcagcg tcggccatct gacggatcgg caccgccgca
      721 tgaccctgcc cttggtaggc ggggaccacg accgcctgca acgcgcagag ggtgttgggt
      781 tcgatcccgt tctcgtattg ctcgatggcg aggggaagca caccgtcgat cccatccggg
      841 aggctcgccg ggttccggtc ccagcggaac gggagggagt tcccgacgca caccacggaa
      901 tcggtgtcgc cgtccacgac gtagaactgg tgctcggggg cgatctcgaa cagctgcccc
      961 cagagctcgt tcacgatcgc gtcgtgcagg aggaactcgg gccacacggg cccaacggtc
     1021 tcgtcccatc gatcttcgag ctccggccgc tcggcatagg tcacgacccg gaggttcatc
     1081 gcagcctgat ctcgagtcgc cgctggctct cgtcctcggc gtgcttccac gcgacggcga
     1141 cgagcaggat cgccaggacc agcgcgaagt tgcccacgag ccacatctgg accgcggccc
     1201 accgctgttc gacgagtgcc tgtgttcccc acggcgccgg gagggccgcg tacgtgggat
     1261 acagcggttc gtcggcgatg tagatcgaga gcgccaggaa cgacatcatc ggcatcgcga
     1321 tgaacagcga cagcatgcgg gccgggtgtg gcatcgggtg cggcgtgggg tcgcggccga
     1381 cgatcggcca ccaatagatc aacgctgccg cgatccacgc ggcgtgctcg gcgcgatgca
     1441 cgaaggttgc acgcagggcg agatcgaaca gcggggacat gtggatcgcg aacgggacgc
     1501 ccacgaacaa cgtgaaccca accactgggt tggacagcgt cctcccgatc cgtcctcgca
     1561 ggatcccggt gatcgatcgg gcgcgaggag cgctgcacgc gcggagggca agggtgatcg
     1621 gggcgcccag cgccaacagc ggtggcgcca cgaacgtcaa caacaggtgc tgggccatat
     1681 gcacgttgaa cgatgcgtcc gcgtaggcat ccacgggtga gatcagggcc agcccaagcg
     1741 cgccgatccc gcaccagaag gcgaacgccc agcgcctggg gaacggggtg ccggcgagtc
     1801 ggagtctccg aaggcctccc gcgtagacga accccaccgc catgagcacg gcaagcgcga
     1861 acgggtcggc gggccaggtg aggatccacg accaggggcc ggttccggga acagcggcgt
     1921 ccatcggcgc atcctcccac ggcctcgcgt tgacgcgtcc ggatcgttcc agttgaatca
     1981 gtcgaacatg gcccgagcgg atgaggtggg ggcggcaccg gcgaggccca tgtctcccgt
     2041 ccgcttcggg acgatcctgt tcttggcgtc ggaactcctg ctcttcggcg gcctgttcgc
     2101 ggcctacttc ggactccgcg cgcgtaccga cccctggccc ccgggcgata tcgaacccga
     2161 ctggcaactg gcgttgttcg gcacggtgct cctcgtggtc tcgagtttct gtctttcccg
     2221 ggggctcgat catgctgcga aggggcgtgt cgacctgctc cgccgttgga cgttggtcac
     2281 gatggtgctg ggtgtcgggt tcctcgcgat ggaggtttgg gactggaccc acgtgtcgtt
     2341 cgagatctcc agcagtccct acggcacgat gtacttcacg cttaccggta tccacggtct
     2401 gcacgtgctg gccggtttgc tggtgctgtc ggtgctgatg ggtcgttcct tccagggtgc
     2461 ctacgacact gggaacctcg acggcgcgca ctccatggcc tactactggc agttctgcga
     2521 cgccacgtgg atcttgatct tcctggtggt gttcgtgctg tcatgacccg tcgctccgcg
     2581 gaagctcgat tgctctcccg cgtccttcgg atcgggctgc tcctggccgt gggcgcccag
     2641 atcgtgtggt cgttcgcacc gtctcgcggt gaggcgtcgt cggcgtcctc gcaagtgggc
     2701 cagaccttgt tcgaagcgaa ctgttcgacc tgccacgggt tgaacgcgga aggcaccgcc
     2761 aacggtccga gccttcaagg ggtaggtccc gccgcggtcg acttcatgtt gacgacgggt
     2821 cgcatgccgc tggcggcgcc cgaagaccaa cccgatcgcc agccaccgaa gttctcgccc
     2881 gatcagatcg acgagatcgt cgcctacgtc gccgagatcg cgcccggcgg cccggcgatc
     2941 cccgatgtcg acacagcccg aggcgatctc ccgaccgggg ccgccctctt
//
LOCUS       DATDBZ010000104        13679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_240635, whole genome shotgun sequence.
ACCESSION   DATDBZ010000104 DATDBZ010000000
VERSION     DATDBZ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13679
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240635"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(528..1439)
                     /locus_tag="VIX62_02530"
     CDS             complement(528..1439)
                     /locus_tag="VIX62_02530"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY5859145.1"
                     /translation="MSGKEFSELFDALSTWGRWGEGDERGALRYASESTVVAGAALVR
                     DGRTFGLGRPLDTRISAHNPRPADHRMTMLGDEGGGPLRFAMDYVGQDYHHPSHSHID
                     ALCHVSYRGSLYNGFKAASTVTADGAAALSVETLHEGLVGRGVLLDVPGVREQSWLEP
                     GESVFRDDLEAAEQAQGVTVGAGDILLVRTGYAQRLAELGDTNGNGSVAGLHPTAMTF
                     VADRRVAALGSDGNSDTAPSTTQGVAFPIHVLAINAMGVHLLDYLILEDMREACASAG
                     RWEFLFVAAPLQIRGGTGSPVNPIAIF"
     gene            complement(1867..3420)
                     /locus_tag="VIX62_02535"
     CDS             complement(1867..3420)
                     /locus_tag="VIX62_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY5859146.1"
                     /translation="MPERYDVIIIGTGAGGGTLAHTLARSGKKILLIERGDFLPRESE
                     NWDPDPVFIQGRYISPETWYDPDGKPFQPQVHYYVGGATKLYGAALYRLRPQDFGELK
                     HIDGVSPAWPLTYDDFEPWYSKAEQLYQVHGNHGEDPTEGHWSQQYPWPAVSHEPRIQ
                     QLADDLAAAGYHPYHAPCGIMLDEAHRAMSTCIRCTWCDGYPCLVHAKSDAESIAVRP
                     AMTSPNVTLLVNTEVVKLETDPTGRSVTGVAVIREGERETYEADIVSLSAGASNTAKI
                     LLSSANDRHPNGLANGSDQVGRNYMFHNSKAVVALSKEPNDTVFQKTLAINDFYLGAD
                     DYDWPVGQIQMVGKSNAEAMKGEEPKLTKLAPHWSLEETARHVVDFWLTTEDLPKAEN
                     RITLADDGNVRLTYDSTNDEEADRLYHELKGILNHAGLADHHVLDKNFYMHMSIPIAG
                     VAHQAGTCRFGNDPATSVLDPDCKAHELDNLYVVDTSFFPSIGAVNPALTAMANAIRV
                     GEHLLERMA"
     gene            complement(3413..4696)
                     /locus_tag="VIX62_02540"
     CDS             complement(3413..4696)
                     /locus_tag="VIX62_02540"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5859147.1"
                     /translation="MVEQAHVVIVGGGFAGLGCAHELAKHPHHARVTLIDRHEYHQFL
                     PLLYQVATFELASGDVGVGLSELYRRHDSIDVVRADVTAADPDARSVTLDDGRTMEGD
                     YLVLAAGSQANFFGTPGTDHVYPLYSLDDARRLRSRILELFEDAERDPSTIEAGGLTF
                     VVVGAGATGTEVSGALADLIHDVMPSRFHDLALDHARVTIVDAGQAVLGPFSPKAHDY
                     ASKVLSERGVEIRLGTKVKEIAEDHVVLSDGDTIATRCVIWGGGIEAAPLAHAVGLKQ
                     GRGGRIDVRDDLTIEDHPRVFVVGDLANIPGPDGRAFPQLGSVALQAGHCAAENILHD
                     VDGKSMQPFAYRDKGIMAMIGHNAAIAEMGEHRHELHGAVGYAAWLGVHAYLMSGTRQ
                     RTDAFIDWAWDEFGKSRVLDTSDEARLHWEGDTDA"
     gene            complement(4696..5649)
                     /locus_tag="VIX62_02545"
     CDS             complement(4696..5649)
                     /locus_tag="VIX62_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEY5859148.1"
                     /translation="MTRVKTRTLAPSPPSGEQFEIQAGDHRAVVTEVGATLRSYTVDG
                     HDVVDGFPVDETSAAGRGQVLAPWPNRLEDGRYTFEGREGRAAWDEPERRNAIHGLVR
                     WLRWQSVERTDRAVELACELPPQPAYPWQLGLGIRYELTPAGMEITATASNGSDAPAP
                     FGIGFHPYLTLGGTAIDDLTLLVPARRTLIGDDRALPAGDQPVAGTDHDFTAARALEA
                     LQLDTAFTDLVRDIDGLARVRLVTAQDSRSATVWMDDTFTHVMVYTGDTLEPEERRRQ
                     GIAIEPMTCPPNAFRSGVDVIRLEPGGAWRGRWGISVEEGL"
     gene            complement(5646..7517)
                     /locus_tag="VIX62_02550"
     CDS             complement(5646..7517)
                     /locus_tag="VIX62_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020104788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY5859149.1"
                     /translation="MSGEQSAAVPSPFPAIDSYAFLSDCHTGALVAPDGSIDWLCVPQ
                     FDAPSVFGALLDREAGSFRLAPFGINVPTSRVYEPGTNVLMTTWRSASGWVEVRDALT
                     MGTRQGEDIVTPHTRPPVDEDADHTLVRTVRCVEGRVEIELVCEPVFDYGRTEAQWEL
                     VGDDRHAADSTGAGVTVRLTTDMALGVEAGRVRARHVLAAGERAYCAVSWADGFVTPG
                     TVDEADARIAATVGFWRGWLDRSRSVDHPWRSMLERSALTIKGLTFMPTGATVAALTT
                     SLPETPGGERNWDYRYTWIRDTTFTLQALHYLQLDWEADEFMQFVADVEPTEDGSLQI
                     MYGIDGRRDLTESTRDDLSGYANARPVRIGNGAFDQRQNDVYGAVLDSVLLHTRQSER
                     LPRRLWPIVEAQSGCAEKVWEQPDQGIWEARGAPQHYVSSKLMCWVAMDRAAKLAAIR
                     GDKDLEASWRKTADEIHADIIKHGVNDAGVLTQHYGSDSLDASVLLAAVFGFLPADDE
                     RLRATVIAIADDLTDDGFVLRYRTDQTDDGLSGKEGTFLICSFWLVSALAIIGEVQRA
                     RDLMERLIRIASPLGLYAEEFDTQTGHHLGNFPQAFSHLAFIEAAGRLILTERLAQI"
     gene            complement(7514..8932)
                     /gene="zwf"
                     /locus_tag="VIX62_02555"
     CDS             complement(7514..8932)
                     /gene="zwf"
                     /locus_tag="VIX62_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014443409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY5859150.1"
                     /translation="MSNGTSHHASTGTRPADVLVVFGITGDLAKVMTFHSLYRLEERK
                     LLDCPIVGVAVDDWTEDKLRAHARRCIADTGQEIDEAVFERFAERLSYVQGDFSDATV
                     YERVAAATKGKSSPVFYLEIPPFLFGTVVKGLSDAGLTTNARVVVEKPFGHDMESARQ
                     LASELHQYIDESQLFRIDHYLGKMGLQEILQLRFANTMLEPVWNRNYLESVQITMAES
                     FGVEDRGHFYDPVGALRDVVVNHLMQVVSAAGMEAPSRGDPVTIKESQVALFRSVVEA
                     DPAHYVRGQYDGYLETEGVAEDSATETYAALRLEIENWRWSGVPFFIRTGKHLAFTQT
                     ELRLVFKQPPKLGFGLRGRRPEPNQLVIKLDPSPGVRFVVEAHRADAPGSEPITLDME
                     FAQEGGEGPTPYEVLLHAAMVGDSTRFTRQDAVEETRRVMQPLLDAPPPVHPYAQGSM
                     GPDAGDALLAGRGRWHEPWVAT"
     gene            complement(9055..9405)
                     /locus_tag="VIX62_02560"
     CDS             complement(9055..9405)
                     /locus_tag="VIX62_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859151.1"
                     /translation="MPGEIVASVLRLSDVTERGDAELGTALRWGYGSAFGLWHGVLRR
                     RLPEPWASLIFGATLMSATFSLFPLLGRTPPPWRWPPDVLATSLATHAAYVVTVALVD
                     GQFDGTSGREEPSM"
     gene            9767..11188
                     /locus_tag="VIX62_02565"
     CDS             9767..11188
                     /locus_tag="VIX62_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY5859152.1"
                     /translation="MIDAGSVHAMLGVNPVPWARAQMAFTLACHIILVPLGVSWALMV
                     LIANYRGLKRDDPTALELAQRWSKYMAVTFAVGAVSGTVLTFEFGLLWPRFMGRWGDA
                     FGIPFAFEGVFFFTEAIFVSIYIFGWRRLKPWTHFWTGVPIVVAGFFGAASVVAANAW
                     MNTPSGFTLDKQGTIVEVDPVSVIFNDAMPLMAAHMLVAAYVVGGFLVASVYAFGMLR
                     GRRDRYHRLGFIIAFSVAAVAIPLQMAVGDSLARWVYNNQPVKFAAIELVPTTASDVP
                     ETLLGHLNEDGTVSGGIPIPGLASWLSDPSTGTKTVVQGLDTVPVHQRPTTREVNTVH
                     LAWDAMVGLGTLLMLLALWYFASWIFRRDMPKGRWFLRIATCAGVLAILAMEAGWVVS
                     EVGRQPWIVYEYMKVEDAATGNTGVWITFVAVVLLYIAVGTTTILVLRGMSRRFRQGG
                     ATDEDVPYGPRTTPGSKVREGDR"
     gene            11185..11790
                     /locus_tag="VIX62_02570"
     CDS             11185..11790
                     /locus_tag="VIX62_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY5859153.1"
                     /translation="MSTAAAVVLFIGVLAYAIFGGADFGAGFWDLLAGGTDRGGRPRT
                     VIDHAIAPVWEANHVWLIFSFVVLWTCFPEAYASITLTLFVPLTIAALGIVLRGSSFA
                     FRKAVLRTSYRRDFGAIFAVSSVLVPYCMGAVVGGIASGRVPAGGRLATPGTVGSTRH
                     PSSVEVWPWSSPPISPPSTSSGTRAACPTRTWWLTSEAAPS"
     gene            11682..12188
                     /locus_tag="VIX62_02575"
     CDS             11682..12188
                     /locus_tag="VIX62_02575"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY5859154.1"
                     /translation="MAVVVAAYLASVYLIWDSRRVSDEDMVAYFRSRAVVAAVVAGVI
                     SIAGIFVLRADARYLFDGLTSRGLPFVVIAAAAGLVSLWTLLRASPRGARILAMVAVA
                     SVVLAWGVAQWDFILPESLTISEAAAPSGTILAVLVAVGLAALLVLPGFVLLYTLDQR
                     GSLPEEGA"
     gene            12373..13197
                     /locus_tag="VIX62_02580"
     CDS             12373..13197
                     /locus_tag="VIX62_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY5859155.1"
                     /translation="MTQPGGTKGDRALLQRWFEGEVVQPMEDFKDPKQEWRRLFSELL
                     GTFFLVLVAAGGGMMGQAFPNTISRTAAVVAPGLMVLAIILFMGKVSGAHLNPAVSLA
                     FWLRGDFPARRVPGYIVVQLAGAALAAWFLQSVIHVSATFGSNYPATGYSSFDAFLME
                     AVLTLGLVSVILGTASGAQNLGIVGAFGVGAYIALAGLWGSPISGASMNPARTFGPNL
                     VGANFTDYWVYVAGPLAGALLAVGAAFALRGAGGGRAGSGAAQGALDTEVAHPKES"
     gene            complement(13250..>13679)
                     /locus_tag="VIX62_02585"
     CDS             complement(13250..>13679)
                     /locus_tag="VIX62_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5859156.1"
                     /translation="PLVGHLVMGKPSREGSRKFWGQMLVVDPGQLPDELLDVDAAHMR
                     RNAADIRELLYLLIGPRGIRRHLVLGDRWSALDVPTLLLVGEHDAFMTPGMLHAWETI
                     ATASPSIDVLHVPGAGHLPWIDEPDRVVAEVQRFLAAPVG"
BASE COUNT         2098 a         4614 c         4535 g         2432 t
ORIGIN      
        1 aactccggtg tctgagggaa gggcggtccc ggcgccgatc ccgacctggc gctcatgagg
       61 tctgttcctg cttcccggcc cgcatgtggg gctccttagg tagggacgtc gctggaccct
      121 tggtatcgac gcgtgcgggc agtgagcccg aggagtggtg gaagactacc gtcggatcag
      181 cccggccaca tccgggcttg cctgtcgagc aaatggtcat cgcctgctgt ccgctgcggt
      241 cctcggtcga tggcgggaac gttcccggta tccatcgtgt ccaacccgca cctgccgttc
      301 ggctatccga gcgctcacgg aattgcaatc cgttggacgc ggatggcgag caacgggaca
      361 acacgaactc caagttcgta cggatcgagg atatgcaggc cgccaccgaa tcctgacccc
      421 gacaacggac cacgcgcgcc gaggccactc cgtcggccgg cgagccctct ttgcggcggc
      481 gatcctcgaa tccgtcgaga cttgatagcg ctcctcggag ccgcgtatca gaagatcgcg
      541 atcgggttta ccggcgagcc tgtccctccg cggatctgca ggggtgcggc aacgaacaag
      601 aactcccatc gtcccgccga cgcgcatgcc tcacgcatgt cctcgaggat caggtagtcg
      661 aggaggtgca cccccatcgc gttgatcgcg agcacgtgga tcgggaacgc cacgccttgg
      721 gtcgtgctgg gtgcggtgtc gctattcccg tccgaaccga gtgccgccac ccggcgatcc
      781 gcgacgaacg tcatcgcggt ggggtgcaat cccgcaacgg atccgttccc gttcgtgtcc
      841 ccaagctccg ccaagcgctg agcgtatccg gtccgaacga gcaggatgtc gcctgcaccg
      901 accgtcacac cctgcgcttg ctcggctgcc tcgagatcgt cgcgaaacac gctctctcca
      961 ggctcgagcc acgactgctc tcgaactcct ggcacgtcca gtaagacgcc acggccgacg
     1021 agtccttcgt gcagcgtctc cacggagagg gccgcggctc cgtccgccgt caccgtgctg
     1081 gccgccttga agccgttgta gagcgagccc cggtagctga cgtggcagag tgcatcgatg
     1141 tgactgtggc tggggtgatg gtagtcctga cccacgtagt ccatcgcgaa tcgcagaggg
     1201 cccccgccct cgtcgcccag catggtcatg cgatggtcgg ccggcctcgg attatgggcc
     1261 gagattcggg tatcgagtgg gcggcccaag ccgaaggtcc gcccgtcacg cacgagtgcc
     1321 gcgccggcaa cgaccgtgga ttcgctcgcg tatcgcagag ccccacgctc gtccccctca
     1381 ccccatcgtc cccacgtgga gagcgcgtcg aacagctcgg agaactcctt cccactcatc
     1441 tcggctcgtt gcctcccgcc cctcttcatc gcgctcgatc catccctcgt cggtcagcgc
     1501 gccacctgat gacagcgact gccccgttgc cgggcgaaga ggcagctgcg gccgccgcct
     1561 cgcctgcgta aacacctgca taggactcgg aggcactcca aggtactcgg tggtactcaa
     1621 cgacacaccg tttccactgc tcaacgccct gatcagcgta ttcaccttgc cttacatggc
     1681 gaaggtcggg atcgagcctt tcagcaccca ccggcaatac ccgccgaggc aggatggcca
     1741 tcacggcccg ggggcaactc cttactgcgt gctccggagg tcgtactgat cgggatcggt
     1801 tggatcgtgc tggcggcccg gggaacgcga tgagagcgcc atccctggcg cgcctctggc
     1861 ccgacgctat gccatccgct cgagcaggtg ctccccaacc ctgatcgcgt tcgccatggc
     1921 ggtcagcgcc ggattcaccg cgccgatgct cgggaagaag ctcgtgtcca cgacgtacag
     1981 gttgtcgagc tcgtgggcct tgcagtcggg atcgagcacg gaggtggccg ggtcgttccc
     2041 gaagcggcac gtgccggcct ggtgcgccac gccggcgatc gggatgctca tgtgcatgta
     2101 gaagttcttg tcgagcacgt gatgatcggc cagtccggcg tggttcagga tccccttgag
     2161 ttcgtggtac aggcggtcgg cctcttcgtc gttcgtggag tcgtaggtca accgcacgtt
     2221 cccgtcatcg gcgagggtga tgcggttctc tgccttgggc aggtcctctg tcgtgagcca
     2281 gaagtccaca acgtgtctcg ccgtttcctc gaggctccaa tgcggcgcga gcttggtgag
     2341 cttcggctcc tcacccttca tcgcctccgc gttggacttg cccaccatct ggatctgccc
     2401 gacgggccag tcgtagtcgt cggctccgag gtagaagtca ttgatcgcca gcgtcttctg
     2461 gaagacggtg tcgttcggct ccttggagag ggccacgacc gccttgctgt tgtggaacat
     2521 gtagttgcga ccgacctgat cggatccgtt ggcgaggccg ttcgggtgcc ggtcgttggc
     2581 agagctcaac aggatcttcg cggtgttgga tgcaccggca gagagggaga cgatgtccgc
     2641 ctcgtaggtc tcccgttctc cttctcggat cacggcgacc cccgtgacgg agcgtccggt
     2701 cgggtccgtt tcgagcttca ccacctcggt gttcacgagc agcgtcacgt tgggcgatgt
     2761 cattgcgggc cggacggcga tggattcggc atccgacttc gcgtgcacca gacacgggta
     2821 accgtcgcac cacgtgcagc ggatgcaggt gctcatagcc cgatgcgcct cgtcgagcat
     2881 gatgccgcac ggtgcgtgat aagggtgata gccagccgcc gcgaggtcgt ccgccagctg
     2941 ctggatccgg ggttcgtggg agaccgccgg ccatgggtac tgctggctcc agtgtccctc
     3001 ggtcggatcc tcgccgtggt tgccgtgcac ctggtaaagc tgctcggcct tcgaatacca
     3061 gggctcgaag tcgtcgtagg tcaacggcca ggcgggggag acgccgtcga tgtgcttcag
     3121 ctcaccgaag tcctggggcc gcagccggta caaggcggct ccgtacagct tcgtggcgcc
     3181 gccgacgtaa tagtggacct gaggttggaa gggcttgcca tccgggtcgt accaggtctc
     3241 gggcgagatg taccgtcctt ggatgaacac cggatcgggg tcccagttct cgctctctcg
     3301 cggaaggaag tccccgcgtt cgatgagcag gatcttcttt cccgatcgag cgagggtgtg
     3361 cgcaagcgtg cctccacccg cgccggtgcc gatgatgatc acgtcgtagc gctcaggcat
     3421 cggtgtcccc ttcccagtgc agcctggcct cgtcggacgt gtcgagcacg cggctcttgc
     3481 cgaattcgtc ccacgcccag tcgatgaacg cgtccgtgcg ctgccgcgtg ccgctcatga
     3541 ggtacgcatg cacgccgagc catgccgcgt agcccaccgc gccatgcagc tcgtggcggt
     3601 gctcgcccat ctcggcgatc gcggcgttgt gcccgatcat cgccatgatc cccttgtcgc
     3661 gataggcgaa cggctgcatc gatttgccgt cgacatcgtg aaggatgttt tccgccgcgc
     3721 agtggcccgc ctgcagcgcg accgatccca gctgcgggaa cgcccggccg tccggaccgg
     3781 ggatgttcgc gaggtcgccg acgacgaaga cacgtggatg atcctcgatg gtcaggtcgt
     3841 cccgcacatc gatccgcccg ccgcgcccct gcttcagtcc gaccgcgtgc gcgagtggcg
     3901 cggcctcgat cccgccgccc cagatcacac agcgagtggc gatagtgtca ccgtcggaca
     3961 gcacgacgtg atcctcggcg atctccttga ccttggtgcc gagccggatc tcgaccccgc
     4021 gctcggagag caccttcgag gcgtagtcat gggccttcgg tgagaacgga ccgagcacgg
     4081 cctgcccggc atcgacgatg gtcacgcgtg cgtggtccag agccaagtcg tggaaccgcg
     4141 acggcatcac gtcgtggatc agatcggcga gggcacccga gacctccgtg ccggtcgcgc
     4201 ccgcgccgac gacgacgaag gtgagccctc ccgcctcgat cgtcgacggg tcacgctcgg
     4261 cgtcctcgaa cagctcgagg atcctcgacc gcagccgccg cgcatcgtcc agggagtaca
     4321 ggggatagac gtggtcggtg cccggggtgc cgaagaagtt cgcctggctc ccggccgcca
     4381 acaccaggta gtcgccctcc atcgttcggc cgtcgtcgag cgtcaccgag cgggcatccg
     4441 gatcggccgc ggtcacatcg gcccgcacca cgtcgatcga atcgtgccgt cgatagagct
     4501 cggagagacc gactcccacg tcgccgctgg cgagctcgaa ggtcgccacc tgatagagca
     4561 gcggcaggaa ctgatggtac tcgtgtcggt cgatcaacgt gacccgcgca tggtgcggat
     4621 gcttcgccag ctcgtgcgcg cacccgagcc cggcgaaccc gcctccgacg atgacgacgt
     4681 gcgcctgctc caccatcaca acccctcctc gaccgagatc ccccagcgcc cgcgccacgc
     4741 cccgccgggc tcgagccgga tcacgtcgac gccggagcgg aacgcattcg gcgggcacgt
     4801 catcggttcg atcgcgatcc cctgccgacg gcgctcctcc ggttcgagcg tgtcgccggt
     4861 gtagaccatc acgtgggtga acgtgtcgtc catccagacc gttgccgagc gcgagtcctg
     4921 ggccgtcacg aggcgcaccc gcgccaagcc gtcgatatcg cgcaccagat cggtgaatgc
     4981 ggtatcgagc tgcagcgctt ccaacgccct cgccgccgtg aagtcgtggt cggtcccggc
     5041 gaccggctgg tcaccggccg gcagcgcgcg gtcgtcgccg atcagcgtcc ggcgcgctgg
     5101 cacgagtaac gtcagatcgt cgatcgccgt cccgcccagc gtcaggtatg gatggaagcc
     5161 gatcccgaac ggcgccggcg cgtcggaccc gttcgaggcg gtcgcggtga tctccatgcc
     5221 ggcgggtgtg agctcgtacc gaatgccgag gccgagttgc cacgggtagg cgggttgcgg
     5281 gggcagctca caggcaagct cgacggcgcg gtccgtgcgc tcgacgctct gccagcgtag
     5341 ccaccgcacc aggccgtgga tcgcgttgcg ccgctccggc tcgtcccagg ccgcgcgtcc
     5401 ttcgcgtcct tcgaaggtgt accggccgtc ctcgaggcgg ttgggccacg gcgccagtac
     5461 ctgtccacga cccgcggctg acgtctcgtc gaccgggaac ccgtcgacca cgtcatggcc
     5521 atcgaccgtg tacgaacgaa gggtcgctcc gacctccgtg accaccgccc gatggtcgcc
     5581 agcttggatc tcgaactgct ctcccgacgg gggcgaggga gccagcgtgc gagtcttgac
     5641 ccgggtcata tctgggcgag gcgctcggtg aggatcaggc gacccgcggc ctcgatgaag
     5701 gccaaatggg agaaggcctg cgggaagttc cccagatgat ggccggtctg cgtatcgaac
     5761 tcctcggcat acagccccaa tggcgatgcg atccggatga gtcgttccat caggtcccgc
     5821 gcgcgctgca cctcgccgat gatcgccagc gcggagacga gccagaacga acagatcagg
     5881 aacgttccct ccttgccgga gagtccatcg tccgtctgat cggtccgata gcgaagcacg
     5941 aagccgtcgt cggtgaggtc atcggcgatg gcgatgaccg tggctcggag ccgctcgtcg
     6001 tcggctggga ggaagccgaa gacggcagcg aggagcaccg aggcatccaa cgagtccgat
     6061 ccgtagtgct gggtcaagac gcctgcgtcg ttgaccccgt gtttgatgat gtcggcgtgg
     6121 atctcatcgg cggtcttccg ccacgatgcc tccaggtcct tgtcgccccg gatcgcggcc
     6181 agcttcgcgg ctcgatccat cgccacccag cacatgagct tggacgacac gtagtgctgc
     6241 ggcgccccgc gtgcttccca gatgccttgg tccggctgct cccagacctt ctcggcacac
     6301 ccggactggg cctcgacgat cggccagagc cgccggggca ggcgctcgct ctggcgggta
     6361 tggagcagca ccgagtcgag caccgcgccg tagacgtcgt tctgccgctg gtcgaacgcg
     6421 ccgttgccga tccgcaccgg acgggcgttg gcgtagcccg acaggtcgtc gcgggtcgac
     6481 tccgtgagat cgcgacgccc gtcgatcccg tacatgatct ggagcgaccc gtcctcggtg
     6541 ggctcgacgt cggcgacgaa ctgcatgaac tcatcggctt cccaatcgag ctggagatag
     6601 tgcaaggcct gcagcgtgaa cgtagtgtca cggatccacg tgtaccggta gtcccaattg
     6661 cgctcgccgc cgggcgtttc cgggagcgat gtggtgagcg cagcgacggt cgcgcccgtc
     6721 ggcatgaacg tgaggccctt gatcgtcaac gccgagcgct ccagcatgct ccgccagggg
     6781 tgatcgaccg agcgcgaccg gtcgagccac cctcgccaga acccgaccgt ggcggcgatc
     6841 cgggcgtcgg cctcatcgac cgtgccagga gtcacgaacc cgtcggccca cgacaccgcg
     6901 caatacgctc gttcaccggc ggcgaggacg tgccgcgccc gaactcgccc cgcctcgacc
     6961 ccaagcgcca tgtcggtcgt gagccgcacc gtgacgccgg ccccggttga gtcggcggca
     7021 tgccgatcgt ccccgacgag ctcccattgc gcttcggtgc gtccgtagtc gaacaccggc
     7081 tcgcagacga gctcgatctc gacccgcccc tccacgcatc ggaccgttcg caccagcgtg
     7141 tgatcggcat cttcatcgac cggcggcctc gtgtgcggcg tgacgatgtc ctcgccttgc
     7201 ctggtcccca tcgtgagggc atcgcgcacc tccacccatc cgctcgccga acgccacgtt
     7261 gtcatgagga cgttcgtgcc gggctcgtag acgcgcgagg tgggcacgtt gatcccgaac
     7321 ggcgcgagcc ggaagctgcc ggcttcccgg tccagcaacg cgccgaagac gctcggcgcg
     7381 tcgaactgcg gaacgcagag ccaatcgatc gatccgtcgg gcgcgacgag cgctccggtg
     7441 tgacagtccg acaggaaggc gtacgagtcg atggccggga agggggaagg caccgcggcg
     7501 ctctgctcgc cgctcacgtt gccacccacg gctcgtgcca gcggccgcga ccggccaaca
     7561 gtgcatcgcc cgcgtcggga cccatcgagc cttgcgcgta cgggtgcacc ggtggcggcg
     7621 cgtccagcag cggttgcatg acccgcctgg tctcttcgac ggcatcctgt cgcgtgaacc
     7681 gtgtgctgtc cccgaccatg gcagcgtgga ggagcacctc gtagggcgtc ggcccttctc
     7741 ctccttcctg cgcgaactcc atgtcgagcg tgatcggctc cgacccgggc gcgtcggcgc
     7801 gatgggcttc gacgacgaac ctgaccccgg gcgaaggatc cagcttgatg accagctggt
     7861 tcggttcggg ccggcgaccg cgcaggccga acccgagttt cggcggctgc ttgaacacga
     7921 ggcggagctc ggtctgggtg aatgcgaggt gcttgccggt cctgatgaag aacggcaccc
     7981 ccgaccaacg ccagttctcg atctcaaggc gcagggcggc gtaggtctcc gtcgccgaat
     8041 cctcggcgac cccttcggtc tccaggtatc cgtcgtactg gccacgcacg tagtgcgcgg
     8101 gatccgcctc caccaccgag cggaacaacg cgacctggga ttccttgatc gtcacggggt
     8161 cgcctcggga aggcgcttcc atgcccgccg ctgataccac ctgcatcagg tggttgacga
     8221 ccacgtcacg gagcgctccg acgggatcgt agaagtggcc gcgatcttcg accccgaagc
     8281 tctccgccat cgtgatctgc acgctctcga ggtagttcct gttccacacc ggctcgagca
     8341 tcgtgttcgc gaatcgcagt tgcaggatct cttggaggcc catcttcccg aggtagtggt
     8401 cgatccggaa gagctgggac tcgtcgatgt actgatggag ctccgatgcg agctggcgcg
     8461 ccgactccat gtcgtgcccg aacggcttct ccacgacgac ccgggcgttc gtggtcagcc
     8521 cggcgtccga gagtcccttc acgacggtac cgaacaggaa gggggggatc tccaggtaga
     8581 agaccggcga gctcttgccc ttggtcgctg ccgcgacccg ctcgtacacg gtggcatccg
     8641 agaaatcgcc ctgcacgtac gacagccgct ccgcgaaccg ctcgaacacg gcctcgtcga
     8701 tctcctgccc ggtgtccgcg atgcacctcc gagcatgggc acgcagcttg tcctcggtcc
     8761 agtcgtcgac cgcgacaccg acgatcgggc agtcgaggag cttacgctct tccaggcggt
     8821 acagggaatg gaacgtcatc accttcgcga ggtccccggt gatgccgaac accaccaaca
     8881 cgtcggcggg ccgggttccg gtggatgcgt ggtgcgatgt tccgttagac atcggtcact
     8941 cccgtgggtc ggcgaacggc tcgatctcag cgcgctccgg tccgtccctc ggagggcgcc
     9001 cacgggacac ctcgctgctg cgtcctcggg taccgaggga tgcggacagg tcggttacat
     9061 cgaaggttcc tccctccccg aggttccgtc aaactggccg tcgaccagtg cgacggtcac
     9121 gacgtaggcg gcgtgcgtgg ccaggctcgt tgccaggacg tctggaggcc accgccacgg
     9181 aggaggcgtt cgcccgagca acgggaacaa tgagaacgtc gccgacatca gggtggcccc
     9241 gaagatcagg ctggcccacg gctccggcag ccgccggcgg agcacgccgt gccacagacc
     9301 gaaggcggat ccgtatcccc accgcaaggc ggtcccgagt tcggcgtcgc cacgctcggt
     9361 cacgtcggag agacgcagga cgctggcgac gatctcgccc ggcaccaggc tgtcgtcaaa
     9421 gtccagttgt ccgtgaacgt ccgggcgcac gacatgttcc gtgcggtatg cgagcgacat
     9481 cgccgccgtg cccgcgaacc cggcgatgac gcttcggacc ggccaagcca gacccacagt
     9541 cgccccccag cttcctgctg gaaccgatcg tttcacgcga gactccgtcg acgccaccct
     9601 acgcagccga gatgaacgtt cggtgacggg gctctctcgg gacctggcct ttcgcgccga
     9661 tcgcgagccg gcgggaaaga cacgattcga aggcacgccc cgacacgtgt atcgtcggct
     9721 cgccgcgagc tcgacggaga gggaacatcg acgtgcgagg gtggccatga tcgatgcggg
     9781 ttccgtgcac gccatgcttg gggtgaaccc cgtgccgtgg gctcgcgcgc agatggcgtt
     9841 caccctcgcg tgccacatca tcctggtacc gctcggggtt tcgtgggccc tgatggtcct
     9901 catcgcgaac tatcgaggac tcaagcgcga tgatccgacc gcgctcgagc tcgcgcagcg
     9961 ctggtccaag tacatggccg tgacgttcgc cgtcggcgcc gtcagcggca ccgtgctcac
    10021 gttcgagttc ggccttctgt ggccgcggtt catgggcaga tggggggacg cgttcgggat
    10081 cccgttcgcc ttcgagggtg tcttcttctt caccgaggcg atcttcgtct cgatctacat
    10141 cttcggatgg cgcaggctca agccctggac gcacttctgg accggggtac cgatcgtggt
    10201 cgccggcttc ttcggtgcag cgtccgtcgt cgccgccaac gcgtggatga acacgccgtc
    10261 cgggttcacg ctcgacaagc agggcacgat cgtggaggtg gatcccgtct cggtgatctt
    10321 caacgacgcg atgcccctca tggcggcgca catgctcgtt gccgcatacg tggtgggcgg
    10381 attcctggtg gcctcggtgt acgccttcgg catgttgcga ggtcggcgag accggtatca
    10441 ccgattgggc ttcatcatcg cgttctccgt cgccgcggtg gcgatcccgc tgcagatggc
    10501 ggtcggcgac tcgctcgcgc gctgggtata caacaaccag ccggtgaagt tcgcggcgat
    10561 cgaactggtt ccgacgaccg cgagcgatgt cccggagacc ctcctcggtc atctcaacga
    10621 ggacggaacc gtctcggggg ggatcccgat acccggtctc gcctcctggc tttcggatcc
    10681 gagcacggga acgaagacgg tcgtgcaggg gctcgacacg gtgcccgttc accagcggcc
    10741 gacgaccagg gaagtcaaca cggtccacct cgcctgggac gccatggtgg ggttgggcac
    10801 gctcctgatg ctcctggcgc tctggtactt cgcctcctgg atcttccgac gcgacatgcc
    10861 gaagggacga tggttcttgc gcatcgccac atgtgcgggc gtcctggcga tcctcgccat
    10921 ggaggcgggt tgggtcgtga gcgaggtggg ccggcagccc tggatcgtgt acgagtacat
    10981 gaaggtggag gacgctgcga ccgggaacac aggcgtctgg atcaccttcg tggcggtcgt
    11041 gctcctctac atcgcggtgg ggacgacgac catcctcgtg ctccgcggca tgagccgacg
    11101 gttccgacag ggcggcgcca cggatgagga tgttccctac ggtccgcgca cgacaccggg
    11161 ctcgaaggtg agggaggggg atcggtgagc accgcagcgg cggtcgttct cttcatcggc
    11221 gtccttgcgt acgcgatctt cggcggcgcc gacttcggtg cgggcttctg ggatctcctc
    11281 gccggaggga ccgaccgcgg aggtcgtcct cgaaccgtga tcgatcatgc gatcgcgccc
    11341 gtctgggagg cgaaccatgt ttggctcatc ttcagcttcg tcgtgctgtg gacctgcttc
    11401 ccggaggcct acgcgtcgat cacgctcacg ctgttcgtgc cgctgacgat cgccgcgctg
    11461 ggcatcgtgc tccgcggctc gagcttcgcg ttccgaaagg ccgtgttgcg caccagctat
    11521 cgccgggact tcggcgccat cttcgccgtc tcgtcggtgt tggttcccta ctgcatgggg
    11581 gcggtcgtcg gtggcatcgc ttccggtcgc gtgcccgcgg ggggaaggct ggcgacccct
    11641 ggcacagttg ggtcaacccg acatccatcc tcggtggaag tctggccgtg gtcgtcgccg
    11701 cctatctcgc ctccgtctac ctcatctggg actcgcgccg cgtgtccgac gaggacatgg
    11761 tggcttactt ccgaagccgc gccgtcgtag ccgccgtggt ggccggcgtc atttcgatcg
    11821 cgggcatctt cgtcctacgg gctgacgccc ggtacctctt cgacgggttg acctcgcgcg
    11881 gactcccgtt cgtggtgatc gccgcggccg ccggcctcgt gtcgttgtgg acgctgctcc
    11941 gggcatcgcc ccggggcgcg cgcatcctcg cgatggtggc cgtcgcctcg gttgtgctgg
    12001 cgtggggcgt ggcgcagtgg gacttcatcc tcccggaaag cctgacgatc tccgaggcgg
    12061 ccgcgccgtc ggggacgatc ctggcggtcc tcgtcgcggt gggcttggcc gcgttgctgg
    12121 ttctcccagg gttcgtgctg ctatacacct tggatcaacg aggctccctg ccagaggagg
    12181 gtgcgtagtc atgtgccctg cgacccacgg tccctgtccc ggccgaacct accaccagct
    12241 cccgcgagga cgcgccggca gggctagggc cgcggagctg aatgtctggc cgcgagatac
    12301 ccgtcacggc gacgagcgac gaattgacat cctcggtccg ccgggtgacg tttccggtaa
    12361 aagggaggac tcatgactca gcctggaggg accaagggag accgtgcgct cctgcagcga
    12421 tggttcgagg gagaagtcgt ccagccgatg gaggacttca aggatccgaa gcaggagtgg
    12481 cgtcggctgt tctccgagct gttgggcacg ttcttcctcg tgctcgtcgc ggcgggcggg
    12541 gggatgatgg gccaagcatt cccgaacacc atcagtcgga cggcggccgt cgtcgcgccg
    12601 gggctgatgg tgctggccat catcttgttc atgggcaagg tgtccggcgc ccacctcaat
    12661 ccggcggtga gcctcgcgtt ctggctccgt ggggacttcc ccgcgcgtcg ggtgcccggg
    12721 tacatcgtcg tccagctcgc cggtgcggcg ctcgcggcat ggttcctcca atccgtcatc
    12781 cacgtgtccg cgaccttcgg ctccaactac ccggcgaccg gctactcctc gttcgacgcc
    12841 ttcctgatgg aggcggtcct cacgctgggg ctcgtaagcg tgatcctggg cacggcctcg
    12901 ggtgcgcaga atctcgggat cgtcggggcc ttcggtgtgg gcgcgtacat cgccctcgcc
    12961 ggcctgtggg gcagtccgat ctccggcgca tccatgaatc ccgcccgcac gttcggtccg
    13021 aacctcgtgg gtgcgaactt cacggattac tgggtgtacg tggccggacc gcttgccggc
    13081 gcgcttctgg cggtcggcgc ggcgttcgca ctccgagggg cagggggtgg acgagcgggg
    13141 tcgggggctg ctcagggagc tctggatacc gaggtcgcac atccgaagga gtcatgagcg
    13201 atcccgtccg tcggcgctcg gtcttgcgga cttcgcacga cgatcgtcct cagccaaccg
    13261 gggccgccag gaagcgctgg acttccgcca cgactcggtc cggctcgtcg atccacggca
    13321 ggtgtccggc cccgggcacg tggaggacat cgatgctggg agaggccgtg gcgatcgtct
    13381 cccatgcgtg caacatcccg ggcgtcatga acgcatcgtg ttcaccgacg aggagcagcg
    13441 tcggcacgtc cagcgcgctc catcgatccc cgagcacgag gtggcgacgg atcccgcgag
    13501 gtccgatcag gaggtagagg agctctcgga tgtccgccgc gttccgtcgc atgtgcgccg
    13561 cgtcgacgtc caggagctcg tcggggagct gcccggggtc gacgacgagc atctggcccc
    13621 agaacttccg gcttccctcg cgcgacggct tccccatcac gaggtgcccg accagcggt
//
LOCUS       DATDBZ010000105         2662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1262327, whole genome shotgun sequence.
ACCESSION   DATDBZ010000105 DATDBZ010000000
VERSION     DATDBZ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262327"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VIX62_02590"
     CDS             <1..362
                     /locus_tag="VIX62_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859157.1"
                     /translation="LGCLTMYAGSRGISASTSRELRRTPAAIVERTGIMVARACASIA
                     AMLDVSEIVLGGVVPSTFGEPFFEALGREFEQRSGLRHLEHLRIRGVGSSRIGPLVAA
                     AAVARHHRLEVSHEASE"
     gene            477..1694
                     /locus_tag="VIX62_02595"
     CDS             477..1694
                     /locus_tag="VIX62_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5859158.1"
                     /translation="MQPTYSADAAVYRQKVQAFLAEKLPPTWAGTGALEGDELARFVD
                     DWRAILYEAGYLAPGWPVEYGGGGLSPLEQVILAEEFTRAGVPTGGPNDVFGIQMLGN
                     TLLLCGTDEQKAHYLPRILSGDDTWCQGYSEPNAGSDLSNLSLRAELDGDQWVLNGQK
                     IWTSAGHLADHIFTLARTDPEAPKHKGISFLLVDMRQPGIEVRPIKMISGESEFNEVF
                     YTDAVCPKENVVGGVNDGWRVAMTLLGFERGEAAAIAPIKFQAELDRLLALARERGVD
                     SDPLIRQRLAWAYAHVQIMRYNGMRVLTQFLGGHRPGPDAAVSKLFWSEYHKLVTELA
                     VDILGAEALAPTGRRPSSAFQTDDAGAPNSSNSWVDTFLNARAGTIYAGSSQVQRNII
                     GEMVLGLPKEPRP"
     gene            1964..2467
                     /locus_tag="VIX62_02600"
     CDS             1964..2467
                     /locus_tag="VIX62_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY5859159.1"
                     /translation="MKARRALVLNASYEPLSIVSSRRAVCLLLADKAELVEADDGVMR
                     SANLTVPAPTVVRLRYMVRVPRRAVAAVSRRAVFARDEYRCQYCGARADSIDHVVPRS
                     RGGSHVWENLAAACRPCNMAKRDRTPDEAGMRLLRPCRAPRGSSWVALAAPGLPDAWR
                     PYLLIAG"
BASE COUNT          404 a          892 c          935 g          431 t
ORIGIN      
        1 cgttgggttg cctcacgatg tacgccggtt cgcggggcat ctcggcgagc acgagtcgcg
       61 agctgcgacg cacgccggcg gcgatcgtcg aacgtaccgg gatcatggtc gcccgagcgt
      121 gcgcctcgat cgcggccatg ctcgacgtct ccgagatcgt gctcggggga gtggtgccgt
      181 cgactttcgg tgagccgttc ttcgaggcgc tcgggcgcga gttcgaacag cgcagtgggc
      241 tcaggcacct cgaacacctc cggatccggg gtgtcgggtc gagccggatc ggcccactcg
      301 tcgcggctgc ggcggtggcc cgccaccatc gactcgaggt ttcgcacgag gcgagcgagt
      361 aggcgagcga gccgaccatc agacacggag gacatgagcg gaaccaagag gagcggagcg
      421 acctatccgc tcatgtcggt agggcttcgg gcccgggtgc tcagtagtgt tgggggatgc
      481 agcccaccta ctcggctgat gccgctgtct accggcagaa ggtgcaggcg ttcctcgccg
      541 aaaagctccc gcccacgtgg gcagggaccg gggccctcga aggcgacgag ctggcgcggt
      601 tcgtcgacga ctggcgggcg atcctctacg aggccgggta cctggcgcca ggctggccgg
      661 tggagtacgg cggtggcggg ctgtcgccgc tcgagcaggt gatcctcgcg gaggagttca
      721 cccgtgccgg cgtgcccacc ggcggaccga acgacgtgtt cggcattcag atgctcggca
      781 acacgctgtt gctgtgcggc accgacgagc agaaggcgca ctacctaccg cggatcctgt
      841 cgggcgacga cacgtggtgt cagggctaca gcgagccgaa cgccggttcc gacctctcca
      901 acctgtcgtt gcgggcggaa ctcgacggcg accagtgggt gctcaacggt cagaagatat
      961 ggacatcggc tggtcacctc gccgaccaca tcttcacgct ggcgagaacc gacccggagg
     1021 ctcccaagca caagggcatc tcgttcctgc tggtcgacat gcgtcaaccc gggatcgaag
     1081 tccgtccgat caagatgatc tcgggtgaga gcgagttcaa cgaggtgttc tacaccgacg
     1141 ccgtgtgccc caaggagaac gtcgtcggcg gcgtcaacga cggctggcgg gtggcgatga
     1201 ccctgctcgg gttcgagcgc ggcgaggcgg cggcgatcgc cccgatcaag tttcaggccg
     1261 agctcgatcg cttgctggcg ctcgcccgcg agcgcggcgt cgattccgat ccgctgatcc
     1321 ggcagcgcct cgcctgggcc tacgcccatg tgcagatcat gcgctacaac gggatgaggg
     1381 tgctgacaca gttcctgggc gggcatcgcc cgggccccga cgctgcggtc tccaagctgt
     1441 tctggagcga gtaccacaag ctcgtcaccg agctcgccgt cgacatcctc ggggcggagg
     1501 cactggcacc aaccgggcgg cgtccttcgt cggcgttcca gaccgacgac gccggcgcac
     1561 ccaactcctc caactcgtgg gtcgacacgt tcctgaacgc ccgggccggc acgatctacg
     1621 ccggttcctc ccaggtgcag cgcaacatca tcggcgagat ggtgctcgga ctcccgaagg
     1681 agccgcgccc ctagcgcgag gccgtggcag gcatcacgga tggtttgcgc gtcgccggag
     1741 cggtcgctcg ccgcccgagt ctgtggacca ccgcgcttcg acaagcacga cggatgacgc
     1801 ctgacggttg gtggcgacgc cctccgttcc tcccgctgcc ggcgaaggac tatctcgggt
     1861 tccgcgccac cactcaatac ggcggaacgc gtcgttcgcc gacccccgaa gatgtggtgg
     1921 actatctcgg atggtgtcgg gagtgggacc gagcgctgcg ccgatgaagg cgaggcgggc
     1981 gctcgtgctg aatgccagct acgagccgct cagcatcgtg tcgtcacgac gcgccgtgtg
     2041 cctcctgctc gccgacaagg ccgagctggt cgaagccgac gacggggtga tgcgatcggc
     2101 caatctcacg gtccctgcac ccactgtcgt gcgactgcgc tacatggtgc gcgtgccgcg
     2161 ccgggcggtg gccgcggtgt cacggcgcgc cgtgttcgcc cgcgacgagt accgctgcca
     2221 gtactgcggc gcccgggccg actcgatcga ccacgtcgtt ccgcgttcgc gaggtggctc
     2281 gcacgtgtgg gagaacctcg ccgccgcctg ccggccgtgc aacatggcca aacgcgatcg
     2341 cacgccggac gaggccggga tgcggctgct gcgtccgtgc cgggcgcccc gcggatcgtc
     2401 gtgggtggcg ctcgccgccc cgggactgcc cgacgcgtgg cggccgtacc tgctcatcgc
     2461 cggttagccc ccgtgcgtcg tccccgatga tcgttcgccg gcgcggcagc gttgccgatc
     2521 tgcacgcaat cgacccgttc gtcgagggtg tggtgacgac acctcgcttg tattggtgtg
     2581 agcccacgac gggcgcgatc gtgctcgggt cgcgccagac gccggagctg ctcgatctcg
     2641 aggcctgcga acggctgggt ct
//
LOCUS       DATDBZ010000106         3297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2082283, whole genome shotgun sequence.
ACCESSION   DATDBZ010000106 DATDBZ010000000
VERSION     DATDBZ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082283"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VIX62_02605"
     CDS             <1..577
                     /locus_tag="VIX62_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5859160.1"
                     /translation="KPGAPRLRDVVMQEATLRVATVDDLAAMEILMKDSIRDLFPAVY
                     TDEQTRSSAEFIGVPDRMLVEDDTFFVIEADDELVACGGWSRRDKLYTGSGETATDAR
                     LLDPATEPARVRAMFVRPDWTRRGLGTMILEASEAAAAAEGFRSLSLMATEPGVPLYQ
                     RYGFTVVEQAQVPMPDGCSITCTAMEKPIAT"
     gene            619..1203
                     /locus_tag="VIX62_02610"
     CDS             619..1203
                     /locus_tag="VIX62_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5859161.1"
                     /translation="MGITQYYTATTIDGYIADEHNSLSWLFEVPAGDDEVGHEVRFAA
                     FMADVGAIAMGSTTFEWVVEHEQLLEHPEKWRAFYGDTPTWVFTHRDLPSFEGADIRF
                     VQGDVRPIHAAMVEAAGDKNIWIVGGGELVGQFADAGLLDEIQLSIAPVTLGAGAPLL
                     PRRLLASDLTLVNLERQGQFVSATYRVTRAADRG"
     gene            1252..2715
                     /locus_tag="VIX62_02615"
     CDS             1252..2715
                     /locus_tag="VIX62_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5859162.1"
                     /translation="MAAAGERPFETPEGQRRYLETLVEASPTAIVSCDPEFIVTSWNP
                     AAERLFGWTRAEALGTHIDDLVGRHEAIRDHAAAMNDRVLLGPTEVLTRRSRKDGTLV
                     DVTIRSVPITMDGTLIGAFALYEDVGELVRQRRFFESLVETSPTAIIMVNTAYEVSLW
                     NPAAERLFGYSSAEAMGMNLDDLVAKDPEMHAEATAWSKEAWDMESFRRIGRRTRKDG
                     SLVDVEILAIREQDGDQPSGYYILYHDVTELEQTRRQLEERIDDQMAELVRTGELARF
                     LPPQVARRLLEGQLQTDESAFERRRITVLFADMVGFTDLAESLEPEELAEVLNGYLRE
                     MSAAVIAFGGSVDNMIGDGVMAVFGAPEEMPEADQAWAAVRAAIEMQSRCQALGTSLR
                     DRGIPADLAIRVGVNTAHCTVGVFGSDVLRAYTAVGFAVNVASRLQTSAEPGTTLCGF
                     RTYALVKDKVRARERGALSVKGARRPVEAWEIVGLAE"
     gene            complement(2720..3286)
                     /locus_tag="VIX62_02620"
     CDS             complement(2720..3286)
                     /locus_tag="VIX62_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5859163.1"
                     /translation="MADLIYSVLASLDGYIADRDGNFDWAEPDDEVHAFVNDLERPIG
                     THLYGRRLYEVMVAWETMDLAAEAPYLRDYQQIWRAADKVVFSTTLTEVRSERTRLEA
                     VFDPAAVREMKASSAANIGIGGPGLATHAIRAGLVDVYQVLVAPVMVGGGTPWLPDDV
                     RLDLELLDERRFRNGTVHLGYREATTAD"
BASE COUNT          576 a         1020 c         1172 g          529 t
ORIGIN      
        1 gaagcctggt gcccctagac ttcgcgacgt ggtgatgcag gaggcgacac tgcgggtggc
       61 cacggtcgat gacctggccg cgatggagat cctgatgaag gactcgatcc gcgatctgtt
      121 ccccgcggtg tacacggacg agcaaacgcg atcgagcgcc gagttcatcg gcgtccccga
      181 tcggatgctg gtcgaggacg acacgttctt cgtgatcgag gccgacgatg agctggtcgc
      241 atgcggggga tggagccggc gcgacaagct ctacacaggt tcgggcgaaa cagcgaccga
      301 tgcacggctc ctggatccgg cgaccgagcc cgcccgggtg cgggcgatgt tcgtgcggcc
      361 agactggacc cgtcgcggac tcggcacgat gatcctcgaa gcgtcggagg ccgcggcagc
      421 ggccgaaggg ttccgctcgc tctcgctgat ggccaccgaa cccggggtgc cgctctacca
      481 gcggtacggg ttcacggtcg tcgagcaggc acaggtgccg atgccggacg gatgctcgat
      541 cacctgcacc gcgatggaga agccgatagc cacctgacag cctcggcggg cggcatcgat
      601 tgtgcgaacc tgcttctcat ggggatcacg cagtactaca cggcgacgac gatcgatggg
      661 tacatcgccg atgagcacaa ctcgttgagc tggctcttcg aggtgccggc gggcgacgac
      721 gaggtggggc atgaggtgcg gttcgccgcg ttcatggccg acgtcggggc gatcgcgatg
      781 ggctcgacca ccttcgagtg ggtcgtcgag cacgaacagc tcctcgagca tccggagaag
      841 tggcgtgcgt tctacggcga caccccgacc tgggtgttca cgcatcgcga cctcccgagc
      901 ttcgagggtg ccgacatccg gttcgtgcag ggcgacgttc gaccgatcca tgcggcgatg
      961 gtcgaggcgg ccggcgacaa gaacatctgg atcgtcggcg gtggggaact cgtggggcag
     1021 ttcgccgatg cgggcctgct cgacgagatc cagctgagca tcgcgccggt gacgctgggt
     1081 gcaggcgcgc cactcctacc gagacgcctg ctcgcgtccg acctcacgct ggtgaacctg
     1141 gaacggcagg ggcagttcgt atccgcgacc taccgcgtga cgcgggccgc ggaccgcggc
     1201 taggggtagg ccgtctagct ctcgacaggt cggagttcta cgcttcactc gatggctgca
     1261 gcaggcgaga ggccattcga gacgccggaa ggccaacgtc gatatctcga gacgttggtc
     1321 gaagcgagcc ccacggccat cgtctcgtgc gatccggagt tcatcgtcac gtcctggaac
     1381 ccagccgccg agcgactgtt cggctggacc agggctgagg cattggggac ccacatcgac
     1441 gacctggtgg gtcggcatga agcgatccgg gaccatgcgg ccgcgatgaa cgatcgtgtc
     1501 ctcctcggac cgaccgaagt tctgacgcgc aggagcagga aggacgggac gctcgtcgac
     1561 gtcacgatcc ggtcggtgcc gatcacgatg gacgggacac tcatcggcgc gttcgcgctg
     1621 tacgaggatg tgggcgaact cgtgcgccag cgccggttct tcgagtcgct ggtggagacc
     1681 agcccgaccg cgatcatcat ggtgaacacc gcgtacgaag tcagtctgtg gaacccagcc
     1741 gcggagcggc tgttcggcta ctcctcggcc gaggcgatgg ggatgaacct cgatgacctc
     1801 gtcgcgaagg acccggagat gcacgccgaa gccaccgcct ggtcgaagga agcatgggac
     1861 atggagtcgt tccgtcggat cgggcggcgg acccgcaagg acggttcgct ggtcgacgtg
     1921 gagatcctgg cgatccgtga acaggacgga gatcagccga gcggctacta catcctgtac
     1981 cacgatgtta ccgagctcga gcagacgcgg cgtcagctgg aggaacgcat cgacgaccag
     2041 atggccgagc tcgtgcgcac cggcgagctc gcacgcttcc taccgccgca ggtagccaga
     2101 cgactgctcg aaggccagct gcagaccgac gaaagtgcgt tcgaacggcg ccgcatcacg
     2161 gtgctcttcg ccgacatggt ggggttcacc gacctggcgg agagcttgga acccgaggaa
     2221 ctcgcggagg tgctgaacgg gtacctgcgc gagatgtccg ccgcggtgat cgccttcggc
     2281 ggttcggtcg acaacatgat cggtgacgga gtcatggccg tcttcggcgc gcccgaggag
     2341 atgccggagg cagaccaggc gtgggcggcc gtccgagcgg cgatcgagat gcagtcacgg
     2401 tgccaggcgc tgggaacgtc cttgcgggac cgaggcatcc ccgccgacct cgcgatcagg
     2461 gtgggcgtca acacggcaca ttgcacggtg ggcgtgttcg ggagcgatgt gctgcgtgcg
     2521 tacacggcgg tggggttcgc ggtcaacgtc gcatcgcgac tgcagacctc ggccgaaccc
     2581 ggcaccaccc tctgcggatt ccggacctac gccctggtga aggacaaggt gagggcgcgg
     2641 gaacggggag cgctctcggt gaaaggcgcg cgccgtccgg tcgaggcgtg ggagatcgtc
     2701 ggcttggctg agtagtcgtt cagtcggcgg ttgtggcttc tcggtacccc aggtgcaccg
     2761 tgccgttgcg gaagcgacgc tcatccagca actcgaggtc cagccggacg tcatcgggga
     2821 gccagggggt gccaccgccc accatgacgg gcgcgacgag cacctggtag acgtcaacta
     2881 accctgcgcg gatggcgtgg gtggcgagac cgggaccgcc gatgccgatg ttcgcggcgg
     2941 acgaagcctt catctcccgg accgcggcgg gatcgaatac cgcctcgagg cgcgtccgct
     3001 cgctgcgaac ctccgtgagt gtcgtggaga acaccacctt gtccgccgcg cgccagatct
     3061 gctggtagtc ccgcaggtac ggcgcctcgg cggccaggtc catcgtctcc caggccacca
     3121 tcacctcgta caggcgtcga ccgtacaggt gggtcccgat cggtcgttcg agatcgttga
     3181 cgaaggcgtg cacctcatcg tcgggctcgg cccagtcgaa gttcccgtcg cggtcggcga
     3241 tgtaaccgtc gagcgaagcg agcaccgagt agatcaggtc agccatgggt gtgggtc
//
LOCUS       DATDBZ010000107         8986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1263226, whole genome shotgun sequence.
ACCESSION   DATDBZ010000107 DATDBZ010000000
VERSION     DATDBZ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263226"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VIX62_02625"
     CDS             <1..359
                     /locus_tag="VIX62_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5859164.1"
                     /translation="VRACVDQDDPAAALAAAKAATGEYASYPAYARQFSLMGLGSHAE
                     AAAAAHRAGTPDDVPDELVHAICLVGGPAAVRGRLDAYRDAGADLPVVYPMLVPGSDP
                     HDSITATIRGLAPATG"
     gene            454..780
                     /gene="fdxA"
                     /locus_tag="VIX62_02630"
     CDS             454..780
                     /gene="fdxA"
                     /locus_tag="VIX62_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005478968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY5859165.1"
                     /translation="MTYTIAEPCIDIKDRACVDECPVDCIYEGPRMLYIQPDECVDCG
                     ACEPACPVTAIFYEDDVPGEWKQFTPVNAEFFNDSVSGLGSPGGAAQVGPAAVDHPVV
                     AGWEAV"
     gene            926..3001
                     /locus_tag="VIX62_02635"
     CDS             926..3001
                     /locus_tag="VIX62_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859166.1"
                     /translation="MRSTAHRRRSAAALLVVGVLLFAAVQVPSAGASGTAAAGTGVTE
                     TLPASTIDRALKASGSVARRDGALTVERIGGTEQTPTLYRLTLTGAYPPRALRYVVEV
                     DAQPVAYGIPGPRGRSLRAITADASVLTGSVSVRYGTSAVPTSSVVPRSSSRPRPSPR
                     AGPTPVMPGPYEVTRRVYDFGDQVFQPSDLGGKVEITADVHFPTGLADGPYPLVLFLH
                     GNHAACFKGNRWSYRWPCKDTWKPLPNYTGYDYVAARLASYGYVVVSVSGNGVNVLGN
                     VVDDSGMRQRGELLEEHLDLWQEWSTVGGAPFGTRFVGKVDMTEVGTMGHSRGGEGVV
                     WQVIVDRERPDPYGIDAVLPLAPVDFTRATINDVPLAVMLPYCDGDVFDLQGIHFYDD
                     SRYLVDGDRSPKHAITVFGANHNFFNTVWTPSSGYPGAFDDGVNECQGRLRPIEERRV
                     GSAYIVDYFRRYLGHDTSVDPVFTGAESPDGIAPARTAVSYLAPDTWQSRQDVDRFTD
                     PSSLARNDLGGAVIPKGVSTYGWCADTFENPCITGLASFADIHLPGLAQGVLGWSDGR
                     ASVRFQLPAGTRAVRRFDALQFRTSINPDYDVNQAFQFQDLSVVLVDGSGDRASVAAS
                     DVGNDALAYPSGLRRYYGHVILQQLRFPLSSFTGIDLGDVRSVQIRFDRTMAGVIDVA
                     DLNFSAGAT"
     gene            complement(3012..6737)
                     /locus_tag="VIX62_02640"
     CDS             complement(3012..6737)
                     /locus_tag="VIX62_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5859167.1"
                     /translation="MRHGAILEAVAFAAERLLLTPDWRDAADEVLARIGAAADVSRAY
                     IIENHFDDEEELLGSVRFEWCAPGIESQKDNPFLNDAPWRDLPRWAAWHAAGEPVVSA
                     VADLPAEERPEFEAQGTLSIAEHPVFTGDWWWGAIGFDDCEEARIWGPAEEQALRATA
                     TLLGAAITRQRVDEQRRRAEDRWGHVVELIPAVTYSDELDSDNVVRMGFVSPQIEQIL
                     GFPPERFLEDARFWFSLMHPDDRARLEAAGAFSAEDTEPFDEEYRMRAADGTYRWVHD
                     GSQAIYRDDGSVDYFLGFMTDITASRAAQHQLRVADERFRTIVEQTPAVTYQEYLNGG
                     VYDGDQVETFVSPHIKGLLGYTQEEWRRPGFWRQATHPDDLPLIDAESDRVIASGERT
                     YRQDYRMVAKDGQIVWFRDESVLIDDADGEPLLWQGVMIDITDRKHAEQQLAQAEERF
                     RLLVERSPAIVYMELPSEGLEAGSVAYVSPQISTLLGVRQEAWQEPGAWAAMMHPDDR
                     ERVVKDGTATRVAGEPWVAEYRMIAADGRVVWFHDESVLILDERGAPSMWQGIMVDIT
                     DQKAAEQRVREAEERHRALVEHIPAIVYAEGLDANPEDLYVSPQVERILGYTPDQWRW
                     TADFWKQHVHPDDLAGVVEINDRVNESGATFLAEYRFRAADGSYRWLRDEAVRVHDDD
                     GRPLFWQGVMLDITDQKRAQQDLREADERYRAVVEHIPAVVYTEAPDADPTKFYISPQ
                     VTDLFGYTADEWTWTADFWRDRIHPDDAETVVAADERSNETLEAYTLDYRFRHRDGHW
                     VWVRDEATFVPELDGEGFWQGFMLDITERKHAEDQLREAELKFRTIVEQNQAIFYTQD
                     IDPDDPAVSRTTYIAPGNTEMLGYTLEDIQDDPTLWRAIVHPDDRDRVFEADAESNLG
                     EGDSAFSMEYRMISKDGRIVWVQDEATLVRLPGKPPYWQGFLLDVTERKEAEAQLERA
                     LDVERDATQRLRALDEMKNTFLQAVSHDLRTPLAAILGLAITLERGDIQIDDDDAKDL
                     AHRIASNARRLDRMVTNLLDLDRLARGIVAPKLESTDVGALLQRVLDETDLIEDSRLV
                     TDLQPVVQPIDAAKVERIVENLLANTVRHTPSNASIWVRVWPEPDGVTIAVEDSGPGV
                     PAEIRDIVFEPFRQGPEAPQHSPGVGVGLTLVRRFAELHGGRAWVQERDGGGASFRVF
                     LPAAGPTELVPPDDHEASRASAGLSALLPPAGLDEVS"
     gene            complement(6912..8042)
                     /locus_tag="VIX62_02645"
     CDS             complement(6912..8042)
                     /locus_tag="VIX62_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEY5859168.1"
                     /translation="MPSSADKTQWRDLYHEVVTTGLCTGCTACIVTCPFHVLGYEDDL
                     PVQLQEEGPDQCSHGEKGCDICTRACPRFRDWEGEIDTSIFGMTRKPEELIGVHKEIT
                     LARATDATSLMTGQDGGVVSALLLWGLERGEIDGACTSKLSDERRWDAEPTVVTDAEG
                     VRATAGSRYTYSANPLALLKAAELGLSKVALVGMGCQASVTGSMEARRVNKWRKKIAW
                     TFGLLCSKSFTYDGLMNEIAQQELGLDLDHLARVNVKGKLLFYTDTGDEVTYSLKQAH
                     RFTRPGCLRCPDFAAEHADISFGGLGQSDGWTLTIVRTDRGADLWSRALADGVIEARP
                     ATEDPKAVELMFKLAARSRERWPSADELPTAAATPGMVADPA"
     gene            8161..8544
                     /locus_tag="VIX62_02650"
     CDS             8161..8544
                     /locus_tag="VIX62_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY5859169.1"
                     /translation="MRTRTTTSIVVMVLLTLSLAACSSDDGDPGGSTPSSAASASGGA
                     AGLTITAEDFSFSPATLAVPSGESTITVTNSGAVEHSFTLDDDGVSEDVEPGESVTVT
                     VDVSADAPFHCKYHPDQMTGTLTIA"
     gene            8643..>8986
                     /locus_tag="VIX62_02655"
     CDS             8643..>8986
                     /locus_tag="VIX62_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY5859170.1"
                     /translation="MSLCVAVVLMLTMAACSSGGDEEPTTGQDGDVLPGTAALAIADL
                     AYEPSVLPVVRGTTEITITNSDTVDHTFTLDTGVIDEPIPAGETVMVAVSLENSAGFH
                     CEIHSQMTGKLQV"
BASE COUNT         1381 a         3090 c         3028 g         1487 t
ORIGIN      
        1 acgtccgggc gtgcgtcgat caggacgatc ccgcggcggc gctcgccgcc gccaaagccg
       61 ccacgggcga gtacgcgagc tatcccgcct acgcccggca gttctcgctg atgggccttg
      121 ggtcacacgc cgaagcagca gcggccgccc accgtgcggg aacacccgac gatgtgccgg
      181 acgaactcgt gcacgccatc tgtctggtgg gcggtcccgc ggcggtgcgg ggccgactcg
      241 atgcctaccg ggatgccggg gcagatctac cggtggtcta ccccatgctg gtccccgggt
      301 ccgaccccca cgactcgatc acggccacca tccgggggtt ggcaccggcc actggatgat
      361 cgctctgcgc ctcgtttgcc gtgtcccgct gggggcgtac actgagggaa cggagattta
      421 caaggtgggc accgtggaaa aggggtttat ggcatgacct acacgatcgc agagccctgc
      481 atcgacatca aagaccgtgc ctgcgtggac gaatgtcccg tcgactgcat ctacgagggg
      541 cctcggatgc tgtacatcca gcccgacgag tgcgtcgact gcggcgcctg cgagccggcc
      601 tgccccgtga ccgcgatctt ctacgaagac gacgtgcccg gggagtggaa gcagttcacg
      661 ccggtcaacg cggagttctt caatgactcc gtcagtggcc tcggttcccc ggggggcgcg
      721 gcgcaggtgg gtcctgccgc cgtcgaccat cccgtcgtcg cgggctggga agccgtctag
      781 cgatccgcag cctccgacgt cacggagccc gggcggtcgg cccgggctca ggtgcatccc
      841 tggacaatcc ccgcgtcggc ctgcgacgat gccgaagccc gcaccgctgg ctctaggccg
      901 gccccacgga aagggatccc ccctgatgcg ctcgaccgcc caccgccgtc gctccgcggc
      961 tgcgctcctg gtggtgggcg tgctgctgtt cgccgcggtt caggtgccgt cggccggggc
     1021 atccgggacc gctgcggcag gcaccggcgt gaccgagacg ctccccgctt cgacgatcga
     1081 ccgggcgctg aaggcatcgg gaagcgtcgc gcgccgcgac ggtgcattga cggtcgagcg
     1141 gatcggcggc accgagcaga cgcccaccct gtaccgcctg acgttgaccg gagcgtatcc
     1201 cccgcgagcg ctgcgatacg tggtcgaggt cgacgcccag ccggtcgcct acggcatccc
     1261 cggcccgaga gggcgcagcc tgcgagccat caccgccgac gcatcggtcc tgaccgggtc
     1321 ggtctcggtc cggtacggca cgtccgcggt cccaacgtcg tcggtcgtcc cacggtcatc
     1381 gtcccggcca cgtccgagcc cgcgcgccgg tccgacgccg gtgatgccgg ggccgtacga
     1441 ggtcacccgg cgcgtctacg acttcgggga ccaggtgttc cagccaagcg acctgggtgg
     1501 caaggtggag atcacggccg acgtgcactt ccccacgggg ttggcggacg gcccctaccc
     1561 cctcgtgctg ttcctgcacg gcaatcacgc cgcatgcttc aagggcaacc ggtggagcta
     1621 tcggtggccg tgcaaggaca cgtggaagcc gttgcccaac tacacggggt acgactacgt
     1681 ggcggctcgt ctcgcgagct acggctacgt ggtggtgtcc gtgagcggga acggcgtcaa
     1741 cgtcctgggc aacgtcgtcg acgacagcgg catgcggcag cgaggtgagc tgctcgagga
     1801 gcacctggat ctgtggcagg agtggtccac ggtggggggc gctccgttcg gtacgaggtt
     1861 cgtcggcaag gtggacatga ccgaggtcgg cacgatgggc cactcgcgcg gtggcgaagg
     1921 tgtggtgtgg caggtcatcg tcgaccggga acgccccgac ccctacggga tcgacgcggt
     1981 gttgccgctg gcgccggtgg atttcacgcg cgcgacgatc aacgacgtgc ccctggcggt
     2041 gatgctgccg tattgcgacg gcgacgtctt cgacctgcag ggcatccact tctacgacga
     2101 ctcccggtac ctggtggacg gcgatcgctc gccgaagcac gcgatcaccg tgttcggggc
     2161 caaccacaac ttcttcaaca ccgtctggac cccgtccagc ggctatccgg gtgccttcga
     2221 cgacggagtc aacgaatgcc aaggccggct ccgtcccatc gaggagcgtc gcgtgggcag
     2281 cgcgtacatc gtcgactact tccgtcgcta cctgggccac gacacctccg tcgatccggt
     2341 cttcacgggt gccgagtccc cggacgggat cgccccggcg aggaccgccg tgagctacct
     2401 cgcgcccgac acctggcaga gccggcagga cgtggaccga ttcaccgatc catcctcgct
     2461 cgcccggaac gatctcggcg gggcggtgat cccgaagggc gtctcgacct acggctggtg
     2521 cgccgacacg ttcgagaacc cgtgcatcac cggcctcgcc tcgttcgccg acatccatct
     2581 gccggggctc gcccaagggg tgctggggtg gtcggacgga cgggcctccg tgcggttcca
     2641 actccccgcg ggcacacgcg ccgtccggcg gttcgacgcg ctgcagttcc gcacgtcgat
     2701 caaccccgac tacgacgtga accaggcctt ccagttccag gacctgtcgg tggtcctggt
     2761 cgatggctcg ggtgaccgcg cgtcggtcgc cgcgtcagac gtggggaacg acgcgctggc
     2821 ctatccctct ggactccgcc gctactacgg tcacgtgatc cttcagcagc tgcggttccc
     2881 gctctcgtcg ttcacgggga tcgatctcgg agatgtccgc agcgttcaga tcaggttcga
     2941 ccggacgatg gccggtgtga tcgatgtggc ggacctcaac ttcagtgcgg gagcgaccta
     3001 ggagcgtcgg ctcaggacac ctcgtccaag ccggccggcg gcaggagcgc cgacaagccg
     3061 gccgaagcac gggatgcctc gtggtcgtcg ggcggcacca gctcggtggg gccggccgcc
     3121 ggcaggaaca cccggaacga ggcaccgccg ccgtcccgtt cctggaccca cgcgcgtccg
     3181 ccgtgcaact cggcgaaccg acgcaccagc gtcagtccga cgccgacgcc aggagagtgc
     3241 tgtggcgctt caggcccctg gcggaagggt tcgaacacga tgtcgcggat ctcggccggg
     3301 accccgggcc cgctgtcctc gaccgcgatc gtgacgccgt cgggttcggg ccagacccgc
     3361 acccagatcg acgcgttcga tggggtgtgt cgcacggtgt tggccagcag gttctccacg
     3421 atgcgctcga ccttcgcggc gtcgatcggt tggacgacgg gttgaaggtc ggtcaccagc
     3481 cgcgaatcct cgatgaggtc ggtctcgtcg aggacacgct ggaggagggc gccgacgtcg
     3541 gtcgactcga gcttgggagc cacgatgccg cgcgcgagcc ggtcgaggtc gagcaggttc
     3601 gtcaccatgc ggtccaatcg gcgcgcgttc gaagcgatgc ggtgcgccag atccttcgcg
     3661 tcgtcgtcat cgatctggat gtccccgcgt tccaaggtga tcgcgaggcc gaggatcgct
     3721 gcaagcggcg tgcgcaggtc gtgtgagacc gcctgcagga acgtgttctt catctcatcg
     3781 agggcccgca ggcgctgggt ggcgtcgcgc tccacgtcga gggcgcgctc gagctgcgcc
     3841 tcggcttcct tgcgctcggt gacgtcgagc aggaatccct gccaataggg gggcttgccc
     3901 ggcagccgca ccagcgtggc ctcatcctgg acccagacga tgcgcccgtc cttgctgatc
     3961 atccggtatt ccatcgagaa ggcggagtcg ccctcaccga ggttgctctc ggcgtcggct
     4021 tcgaacaccc gatcccggtc gtcggggtgg acgatcgccc gccacaacgt cgggtcgtcc
     4081 tggatgtcct ccagcgtgta gccgagcatc tcggtgttgc cgggggcgat gtaggtggtg
     4141 cgcgacaccg ccggatcgtc gggatcgatg tcctgggtat agaagatcgc ttggttctgc
     4201 tccacgatcg tgcggaactt cagctcggcc tcccggagct gatcctcggc atgcttgcgt
     4261 tcggtgatgt cgagcatgaa cccctgccag aagccctcac cgtccagctc cggcacgaac
     4321 gtggcctcat cacgcaccca cacccagtgg ccgtcccggt gccggaatcg gtagtccagc
     4381 gtgtaggcct ccagcgtctc gttggaccgc tcgtccgcgg cgacgaccgt ctccgcgtca
     4441 tccgggtgga tgcgatcgcg ccagaagtcg gcggtccagg tccattcgtc ggccgtgtag
     4501 ccgaacaggt cggtgacctg cgggctgatg tagaacttgg tcggatcggc atccggggcc
     4561 tcggtgtaga cgaccgccgg gatgtgttcg acgacggcgc ggtagcgctc atcggcctca
     4621 cgcaggtcct gctgagcacg cttctgatcg gtgatatcca acatcacgcc ctgccagaac
     4681 aacggccggc cgtcgtcgtc gtgcacgcgg accgcttcgt cgcgcagcca ccggtaggac
     4741 ccgtcggcgg cccggaaccg gtattcagcg aggaaggtgg caccggattc gttcacccga
     4801 tcgttgatct cgacgacgcc ggccaggtcg tcggggtgca cgtgctgctt ccagaagtcc
     4861 gccgtccacc gccactgatc gggggtgtag cccaggatcc gttccacctg cggactcacg
     4921 tagagatcct cggggttggc gtcgaggcct tccgcgtaca cgatcgcggg gatgtgttcg
     4981 accagcgcgc ggtgccgctc ctcggcttcg cggacacgct gctcggccgc cttctggtcg
     5041 gtgatgtcga ccatgatgcc ctgccacatc gagggcgcgc cccgttcgtc gaggatcagg
     5101 accgactcgt cgtggaacca caccacccgt ccgtcggcag cgatcatccg gtactcggcc
     5161 acccatggct ccccggccac cctcgtggcg gtgccgtcct tgacgacacg ctcgcggtcg
     5221 tcgggatgca tcatcgccgc ccaggcgccg ggctcctgcc acgcctcctg tcgcacaccc
     5281 agcaaggtgg agatctgcgg gctcacgtag gccacggatc ccgcctccaa cccctcggac
     5341 ggcagttcca tgtacacgat ggccggtgag cgctcgacca gcagccggaa ccgctcctcg
     5401 gcctgggcga gctgctgttc cgcgtgcttg cggtcggtga tgtcgatcat cacgccctgc
     5461 cacagcagcg gctcgccatc ggcgtcgtcg atcaggaccg actcgtcgcg gaaccacacg
     5521 atctgtccat ccttggcgac catccgatag tcctgccggt aggtccgctc tccggaagcg
     5581 atcacccggt cgctctcggc atcgatcagc ggcagatcgt cggggtgggt ggcttgccgc
     5641 cagaacccgg gacgtcgcca ctcctcctgt gtgtacccga gcagcccctt gatgtgcggg
     5701 ctcacgaacg tttcgacctg gtcgccgtcg tagacgcccc cgttcagata ctcctgatac
     5761 gtcaccgccg gggtctgttc cacgatcgtg cggaaccgct cgtcggcgac ccggagctgg
     5821 tgctgggcgg cgcggctggc ggtgatgtcg gtcatgaacc cgaggaagta gtcgacgctg
     5881 ccatcgtcgc ggtagatcgc ctgggagccg tcgtggaccc accggtaggt cccgtcggca
     5941 gcacgcatcc ggtattcctc gtcgaacggc tcggtatctt cggcgctgaa cgctcccgcc
     6001 gcctccaacc gcgcccgatc gtccgggtgc atcaggctga accagaagcg ggcatcctcc
     6061 aggaaccgct cgggcgggaa gcccaggatc tgctcgatct gcggactcac gaatcccatc
     6121 cgcacgacgt tgtccgagtc cagttcgtcg gagtaagtga cggcggggat cagctccacg
     6181 acgtggcccc atcggtcttc agcccgccgg cgctgttcgt cgacccgttg acgcgtgatc
     6241 gccgcgccca acaacgtggc ggtggcgcgc aatgcctgct cctcggcggg tccccagatc
     6301 ctggcctcct cgcaatcgtc gaaaccgatc gcgccccacc accaatcccc ggtgaacacg
     6361 ggatgctcgg cgatcgagag ggtgccttgg gcttcgaact ccggtcgctc ctcggccggg
     6421 aggtcggcga cggccgaaac caccggctcc cctgcggcgt gccatgcggc ccagcgcggc
     6481 aggtcgcgcc acggcgcgtc gttcaggaac gggttgtcct tctgcgactc gatcccgggg
     6541 gcgcaccact cgaagcgcac gctgcccagc aactcctcct catcgtcgaa atggttctcg
     6601 atgatgtagg cgcgggagac gtcggcggcg gcaccgatcc gggccaggac ctcgtcagcg
     6661 gcatcccgcc agtcgggggt gagcaacaga cgctcggccg cgaacgccac ggcttccagg
     6721 atggcgccgt gtcgcaaggc cgatgtccgt ggttttcgcg ccgctcgcgc cgtatccgac
     6781 aaccgctcgc tccccgcccg ggttcgggcg cgtcgatcac aacccctcgt ggggccctga
     6841 ggggattatc ggccggagat ggtcccggtg aagccctcga cgataggggt ttcggcgatg
     6901 gttcgaacgg gctaggcggg gtctgcgacc atgcccggtg tcgcggccgc cgtggggagt
     6961 tcgtcggcgg acggccatcg ttcccgcgac ctggcggcca gcttgaacat cagttccacc
     7021 gctttgggat cctccgtggc cggacgagcc tcgatgacgc cgtccgccag cgcccgcgac
     7081 cacaggtccg cccctcggtc ggtgcgcacg atcgtcaggg tccatccgtc ggactgaccg
     7141 agacccccga acgagatgtc ggcgtgctct gcggcgaagt cggggcagcg cagacatccg
     7201 ggccgggtga accggtgtgc ctgcttcagc gagtaggtca cctcgtcgcc ggtatcggtg
     7261 tagaacagca acttgccctt cacgttcacg cgggcgaggt ggtccaggtc gagtccgagc
     7321 tcctgttggg cgatctcgtt catgagcccg tcgtaggtga aggacttcga gcacagcagc
     7381 ccgaaggtcc acgcgatctt cttgcgccac ttgttgaccc gccgggcctc catcgagccg
     7441 gtgacgctgg cctgacagcc catgccgacg agcgcgacct tcgacaggcc caactcggcg
     7501 gccttgagca gagccaaggg gttggccgaa taggtgtagc gagagccggc ggtggcccga
     7561 acgccttcgg catcggtgac gaccgtgggc tcggcgtccc agcggcgctc atcggagagc
     7621 ttcgaggtgc acgcgccgtc gatctcaccg cgctccaacc cccacagcag cagggcgctg
     7681 accacgccgc cgtcctggcc ggtcatcagt gacgtggcgt cggtggcacg agcgagggtg
     7741 atctccttgt ggacgccgat caactcttcg ggcttgcgcg tcatgccgaa gatcgacgtg
     7801 tcgatctcgc cttcccaatc gcggaaacgc gggcaggcgc gggtgcagat gtcgcagccc
     7861 ttctcgccgt gcgaacactg gtcgggcccc tcttcctgga gctggaccgg aaggtcgtcc
     7921 tcgtacccga gcacgtggaa cgggcacgtc acgatgcacg ccgtgcagcc cgtgcagagc
     7981 ccggtcgtta cgacctcgtg gtacaggtct cgccattggg tcttgtcggc ggacgacggc
     8041 atgatgcgcg cagcatagcc caggtccggg caggcccctg agggtacccc ccgaggaaac
     8101 cggaaccgcc ccccgtccgt agtacccccg ttcgagactg cagacggggg agaggcaagc
     8161 atgcgaacgc gaacgacgac atccatcgtg gtgatggtgc tgctcacgct gagcctggcc
     8221 gcgtgcagca gcgacgacgg cgatccgggc ggctcgaccc cctcgagcgc agcgagcgcg
     8281 tccggtgggg cggccgggct cacgatcacg gccgaggact tctcgttctc gccggctacc
     8341 ttggccgtgc cctcgggcga gagcacgatc accgtgacga actccggggc ggtcgagcac
     8401 agcttcacgc tcgacgacga cggcgtcagc gaggacgtgg agcccggcga gtccgtgacg
     8461 gtgacggtgg acgtcagcgc cgacgcgccc ttccactgca agtaccaccc cgaccagatg
     8521 accggcaccc tcaccatcgc ctgatcgcgt cggcttcccg agtggactag gctgctgcgg
     8581 gaacgatcga ccgatcacga gggggagcga tgcgccaccg aactcgccac ggaaccagtt
     8641 gggtgtctct gtgcgtcgcg gtggtgctga tgctcacgat ggccgcctgc tcgagcggcg
     8701 gtgacgagga acccaccacc ggccaggacg gcgacgtgct gccgggcacg gcggcactcg
     8761 cgatcgccga cctggcgtat gagcccagcg tgttgcccgt cgtacgcgga acgaccgaga
     8821 tcacgatcac caatagcgac acggtcgatc acacgttcac cttggacacc ggggtgatcg
     8881 acgaacccat cccggccggc gagaccgtga tggtggccgt gagcctggag aactcggcag
     8941 ggttccactg cgagatccac tcacagatga cgggcaagct ccaggt
//
LOCUS       DATDBZ010000108         2342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_652759, whole genome shotgun sequence.
ACCESSION   DATDBZ010000108 DATDBZ010000000
VERSION     DATDBZ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652759"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VIX62_02660"
     CDS             <1..625
                     /locus_tag="VIX62_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5859171.1"
                     /translation="AVGDAMTADPIEAPLRSTPMATPPITYVRHAMPMVEGDAPSTEW
                     HLDEPVRAAAQEWAGRLEVGRGIGVLVSSTEPKALETARAIAARWDSSVTEDPRLREA
                     QRPWIGTGYRAVAHRYLRGERPEGWEEHVAVAERMADAVADALAAAGDRTAIVVSHGL
                     ALSIHLGDRLGPDFDRETFWSRLAFPDAWALDADDVLHRSLPPAPIS"
     gene            complement(618..1352)
                     /locus_tag="VIX62_02665"
     CDS             complement(618..1352)
                     /locus_tag="VIX62_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859172.1"
                     /translation="MPDSTTVATEEPERGFEVRGRKPPGPHTLKLLAVPIIGLIVISN
                     VGDALAPQLVNSHPLQLLAMNARNRNLILVTNQLDAASYYVVGTLRLLVADPLFFLIG
                     YWYGDTALDWMERRTKSFGKTLRQWERWFGKAAYPIVFIAPNQYICLFAGAAGMSVAG
                     FFVANISGTLVRLYLIRRLGEAFEAPIDDVLGFIRDYRTPLLIVSVVVFAGIMVNELR
                     QTKEDVDAIADAADDGEAAEPEPPVS"
     gene            1398..>2342
                     /locus_tag="VIX62_02670"
     CDS             1398..>2342
                     /locus_tag="VIX62_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5859173.1"
                     /translation="MQGLMQDVPLTLPHIFGRAETLFFDKEIVTATATGLERTTYGEW
                     AARTRRLGGVLDDLGISETGRAATFAWNTARHLELYFGIPCTGRVLHTLNIRLFPEQL
                     VYIVNHAEDEVIFVDRSLIGLLWPLVDQFETVRHIVVMDDGKGEVPSPDGGRPLHDYE
                     DLLADASPAQFDVTDENLAAAMAYTSGTTGNPKGVVYSHRSTYLHTLACMSAGSLGVQ
                     ESDRILPVVPMFHANAWGLAHAAVASGASLVMPGSDLSAPSLAKLIEEERVTIAAGVP
                     TIWMGVLPELKGRDTSALRAIPCGGSAVPRALSEAYRAQ"
BASE COUNT          363 a          783 c          844 g          352 t
ORIGIN      
        1 cgcggtgggc gacgccatga ccgccgaccc tatcgaggcg ccgctacggt ccacaccgat
       61 ggccacaccc cccatcacct acgtgcgcca cgccatgccc atggtggagg gagacgcccc
      121 ttcgacggag tggcatctgg atgagcccgt gcgagcggcg gcgcaggagt gggcgggccg
      181 gctcgaagtg gggaggggca tcggtgtcct cgtttcgagc acggaaccga aggcgctcga
      241 gaccgcgcgg gccatcgcgg cgcgttggga cagctcggtg accgaggacc cgcggctgcg
      301 cgaggcgcaa cgaccgtgga tcggcacggg ctaccgggcg gtcgcgcacc gctacctgcg
      361 cggcgaacga ccggaagggt gggaggagca cgtagccgtg gccgagcgca tggcggacgc
      421 ggtggccgat gcgctcgcgg ccgccggaga ccgcaccgcc atcgtcgtgt cgcacgggct
      481 ggcgctctcg atccacctcg gcgatcgact ggggccggac ttcgaccgcg agaccttctg
      541 gagccggctc gcgttcccgg acgcgtgggc gctcgacgcc gacgacgtgc tgcaccgctc
      601 gttgccaccc gctccgatca gctgacgggt ggctcaggct ccgcggcttc tccgtcgtcg
      661 gcggcgtcag cgatcgcgtc gacgtcttcc ttggtctgcc gcagctcgtt gaccatgatc
      721 ccggcgaaga cgaccacgga gacgatcagc aacggggtgc ggtagtcgcg gatgaacccg
      781 agcacgtcat cgatgggggc ctcgaaggcc tcgccgaggc ggcggatgag gtagaggcgc
      841 accagcgttc cgctgatgtt ggcgacgaag aacccggcga cgctcatccc cgccgccccc
      901 gcgaacaggc agatgtactg gttgggcgcg atgaacacga tcgggtacgc ggccttgccg
      961 aaccaccgct cccattggcg cagcgtcttg ccgaacgact tggtgcgccg ctccatccag
     1021 tcgagcgcgg tgtcgccgta ccagtagccg atgaggaaga agagggggtc ggccacgagc
     1081 aggcgaaggg tgccgacgac gtagtagctc gcggcgtcga gctggttggt gaccaggatc
     1141 aggttgcggt tgcgagcgtt catggccagc agctgcaacg ggtggctgtt gaccagctgc
     1201 ggagcgaggg catcgccgac gttcgagatg acgatcagac cgatgatcgg caccgcgagg
     1261 agcttcaggg tgtgcggccc cggtggcttg cggccacgca cctcgaagcc gcgctcaggt
     1321 tcctccgtcg ccacggtggt tgagtctggc atctcgtagt ctctgcgacg ccgtcgactc
     1381 tcagggggac tcgggccatg caaggtctca tgcaggatgt tccgctcacg ctgccgcaca
     1441 tcttcggccg agcggagacg ctgttcttcg acaaggagat cgtcaccgca acggcgacgg
     1501 ggttggagcg aaccacctac ggggagtggg cggcgcgcac tcgtcggctt gggggcgtgc
     1561 tcgacgacct tggcatcagc gagaccgggc gcgcggccac gttcgcgtgg aacacggcgc
     1621 gccacctcga gctgtacttc ggcatcccgt gcacgggtcg ggtgctgcac acgctgaaca
     1681 tccggctgtt ccccgagcag ctcgtctaca tcgtgaacca cgccgaggac gaggtgatct
     1741 tcgtcgaccg gtccctcatc ggcctcctct ggccgctggt cgaccagttc gagaccgtgc
     1801 ggcacatcgt ggtgatggac gacggcaagg gcgaggtgcc gagccccgac ggcggtcggc
     1861 cgctgcacga ctacgaggac ctgctcgccg acgcgtcgcc ggcgcagttc gacgtgaccg
     1921 acgagaacct cgcggcggcg atggcctaca cgagcggtac gaccggcaac ccgaagggcg
     1981 tcgtctactc acaccgatcg acgtatctgc acacgctcgc ctgcatgtcg gccggcagcc
     2041 tgggcgtgca ggagagcgat cgcatcctgc cggtggtgcc gatgttccac gccaacgcct
     2101 ggggcctggc gcacgccgcg gtggcgagcg gcgccagcct cgtgatgccc ggatccgacc
     2161 tgtccgcgcc gtcgctcgcc aagctcatcg aggaggagcg ggtgacgatc gccgccgggg
     2221 tgccgaccat ctggatgggc gtgctgccgg agctcaaggg tcgggacacg tccgcgctgc
     2281 gggccatccc gtgcggtggc tccgcggtgc cgcgcgccct ctccgaggcc taccgggccc
     2341 ag
//
LOCUS       DATDBZ010000109         6691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_446334, whole genome shotgun sequence.
ACCESSION   DATDBZ010000109 DATDBZ010000000
VERSION     DATDBZ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446334"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="rdgB"
                     /locus_tag="VIX62_02675"
     CDS             <1..600
                     /gene="rdgB"
                     /locus_tag="VIX62_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY5859174.1"
                     /translation="IASRNAHKLRELGRICADWPVTWITVDNHDPSAFPEVEETGDTY
                     LENALLKARAISEALGVPAIADDSGIEVDALGGKPGPRSARYSGEDATDEQNLEAMMQ
                     AMRGIPSGGRTARYRCIAAIAWPDGRELHTDGTCEGTLVNKRHGERGFGYDPIFVPEG
                     WDETMAQLTDEQKDRISHRGRAFRGLRQLLDEASLPDSR"
     gene            627..1787
                     /locus_tag="VIX62_02680"
     CDS             627..1787
                     /locus_tag="VIX62_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5859175.1"
                     /translation="MSQTNVYEQGLEPRVLRNTRGFFDGRSKLLVVVLGLAFIAVVTI
                     LDYLTGPSLSLSLLYLMPIGLVTWNLGRRWGSATVVVATIAGILADVLAAPGTGQTDP
                     VPYWNAIVRFAVFLAFAMLLDTLRSIIDAQWERVEVEAGRSSDLREMNDVKDTLLHAV
                     SHDLKGPLAGIIGAMQTIRRDDDLHLTEEEKESLYQVIEQSGRKMNRLIDDLLDLDRI
                     DRGKLHPQRKPTDVGELARRVVRESTVLDGHPMRIEADRVLVDVDPGKVERVIDNLLV
                     NASRHTPPGTPVHVRVMGRPNGILLVVEDEGPGVPDEMKGVLFEAFRQGDGASGRGMG
                     IGLSLVQRFAELHGGTAHIEDGETGGARFVVALPGDVTELAAPEEPPSLQAV"
     gene            complement(1823..2224)
                     /locus_tag="VIX62_02685"
     CDS             complement(1823..2224)
                     /locus_tag="VIX62_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859176.1"
                     /translation="MRKNVVLLAVIASLGLAVVAMDAGAETPRGGATLSQRVADLEDR
                     ADRQAHAIAELRTENARQQTQIAALTKFKKETLRWKAGITKKTSKLRSTGRYVGPVDN
                     GQVQVGQGASACANQLARWNATAKSLGCETA"
     gene            2413..3090
                     /locus_tag="VIX62_02690"
     CDS             2413..3090
                     /locus_tag="VIX62_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859177.1"
                     /translation="MKGRIVVVLGLVIAAVSATSASAGELVAWDSFSDGEATISDSGG
                     AWTQVNHSWSVQDGQAVAAADRTRIGYAVMATPVSDDFTVQADITLSPTDLRAAPALV
                     TNWVDNKNNMVTKLEVTPGHPGGMLAVGDQINGQIRSTLCKVERLGLVNGVAYRLSVT
                     RAGDIVTGSVTSLEDGSVIGSCSAMLSSVASSAFGAGTGQGLRVKIKDDEDDGGSRWD
                     NFAVFTN"
     gene            3114..3626
                     /locus_tag="VIX62_02695"
     CDS             3114..3626
                     /locus_tag="VIX62_02695"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5859178.1"
                     /translation="MSGSAMKDAFGHHVWATTRLIDVCIGLPPEQLATAVPGTYGSIL
                     DTQRHLVGSDAWYLRCITNDAKAAIDEDHMDLRELRSAMERNGARWSQLLADELDPDT
                     VITQVDHEDGYQKDATVGIRFAQALHHGTDHRSQICTALTSLGVEPPPIDVWDFGVEH
                     GRVVEVFPTS"
     gene            3674..4180
                     /locus_tag="VIX62_02700"
     CDS             3674..4180
                     /locus_tag="VIX62_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY5859179.1"
                     /translation="MGTTGGRRRLRRTLDETDVLIIEALNQDGRRSFAALARDLDLNE
                     ATVRSRFARLTRRGVVQVVVLADALALGLVFAEVGIKIRGGTVTSVVTELQKVPEIDY
                     IAVCAGSFDLLVEIVCVDNEHMLQVLEERIRPVDGVDTVETFTILDVPKHSYRWTKLL
                     ASNVSFPE"
     gene            4336..5490
                     /locus_tag="VIX62_02705"
     CDS             4336..5490
                     /locus_tag="VIX62_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5859180.1"
                     /translation="MNENEAVAWAIEELVAQIEIRSISDHEDRMMAYLDERCRELGFP
                     TTLQPIEGAGPNVVVRWDGRNDPPDLLLTAHTDTIDPAWDWRDVATVEGTVVTGLGAQ
                     DDKGCGVAILLGAVLAREAGVDLSETSVALGFVVDEEVGGKGSRALADSLRPRFVVAS
                     EGSELDIATVETGFIDGWVRVSGVTMHGSLPEEADNAVEKAARLLLALHEAPFTSISH
                     PIAGPNTVTAQRIESSSAMNAIPDVANMYISCRVFGEPSLDETRKEIEAICRRFGATF
                     ELHDHGGWWETPQDAPLVQAMIRASEQAIGRTPGFTRMPAWTDAHSFFDRCGSEVVVF
                     GPGHLRAAHRPDEHLDARELVRCARVFAALIADTASLSTAEPTRSKEGVR"
     gene            5487..6593
                     /locus_tag="VIX62_02710"
     CDS             5487..6593
                     /locus_tag="VIX62_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5859181.1"
                     /translation="MKSPWTRCFALVVALALTAAACGSDGDGDDSASAAGGEQSTGPV
                     SGELTFFAYEDAFEPELLDPFEAANPDLDVRTAAFSSGDETVTKLQSGFQADVVNVCV
                     EDTTRMVNLGLLQPIDTGRIEAWDEMFPAFTGLPGVEVDGQTYLVPMVGGTSGIMYNS
                     QDIPDGFDSYASVFDEAYAGRIGMDDDPLSGIAIGAMAAGITDPMNLDDAELEQVKQY
                     LIEKKPLLRSFVKGDADIAQLFKSGEIDVVVPGYKGSTETLQQEGEPVDYSLADEGQL
                     TWTCGYAIGANAANVDAAYALINHYAQPETQAWQAENFFYLVSNQDTLDAVSPKIVDE
                     AGLEDPGSFEHAIPYAIPENYDAWQEAWREIKAA"
BASE COUNT         1103 a         2207 c         2333 g         1048 t
ORIGIN      
        1 atcgcctcac gcaacgcgca caagctgcgc gaactcggcc ggatctgtgc ggactggccc
       61 gtcacctgga tcacggtgga caaccacgac ccctccgcgt tccccgaggt ggaggagacg
      121 ggcgacacct acctcgagaa cgccctgttg aaggcgcgcg cgatctccga ggcgctcggc
      181 gtgccggcga tcgccgacga ttccgggatc gaggtggacg cgttgggcgg caagcccggc
      241 ccgcgctcgg cgcggtactc gggggaggac gccaccgacg agcagaacct cgaggccatg
      301 atgcaggcga tgcgggggat cccgtcgggc ggccgcaccg cccggtaccg gtgcatcgcc
      361 gcgatcgcct ggccagatgg acgggagctg cacaccgacg gcacctgcga gggcacgctg
      421 gtgaacaagc gccacgggga gcgggggttc ggctacgacc cgatcttcgt acccgagggg
      481 tgggacgaga cgatggccca gctcaccgac gagcagaagg accgcatcag ccaccgcggc
      541 agggcattcc gagggctgcg tcagctcctg gacgaggctt cgctacccga ctcaaggtag
      601 gtgccgccga ggccgacgct agttcggtga gtcaaacgaa cgtgtacgaa cagggtctgg
      661 aaccgcgcgt cctgcgcaat acgaggggat tcttcgacgg acggtcgaag ctgctcgtcg
      721 tcgtgctggg cctggccttc atcgcggtgg tcacgatctt ggactacctc accgggccgt
      781 cgctctccct ctcgctgctc tacctcatgc cgatcgggtt ggtgacgtgg aacctggggc
      841 gccggtgggg ctcggcgacg gtcgtggtgg ccacgatcgc cggcatcctc gccgatgtgc
      901 ttgccgctcc gggcaccgga cagaccgacc ccgttccgta ctggaacgcc atcgtgcggt
      961 tcgcggtgtt cctggcgttc gcgatgttgc tggacaccct ccgctcgatc atcgacgcgc
     1021 agtgggaacg cgtggaggtc gaggccgggc gctcgagcga cctgcgagag atgaacgacg
     1081 tgaaggacac gttgttgcac gcggtctcgc acgacctgaa gggcccgctc gccggcatca
     1141 tcggcgcgat gcagacgatc cgtcgcgacg acgacctgca cctgaccgaa gaggagaagg
     1201 agagcctgta ccaggtgatc gagcagagcg gtcgcaagat gaaccgcttg atcgacgacc
     1261 tcctggacct cgatcggatc gatcgcggca agctgcaccc acagcgcaag cccaccgatg
     1321 tcggcgaact ggcgcgccgg gtggtgcgcg agtcgaccgt gctcgacgga cacccgatgc
     1381 gcatcgaggc cgaccgcgtc ctggtcgacg tcgacccggg caaggtggag cgcgtgatcg
     1441 acaacctgct ggtgaacgcg tcgcggcaca cgccgcccgg gacgccggtg cacgtccgcg
     1501 tgatgggacg cccgaacggg atcctgctcg tggtcgagga cgaaggcccg ggcgttccgg
     1561 acgagatgaa gggcgtgctc ttcgaggcct tccgtcaggg tgacggggca agcgggcggg
     1621 gcatgggcat cggtctgtca ctggtgcagc ggttcgccga actccacggc ggcacggcgc
     1681 acatcgagga cggcgagacg ggcggtgcac ggttcgtcgt ggcgctgccg ggcgacgtca
     1741 ccgaactcgc cgcgccggag gaaccgccgt cgctccaagc cgtctgaggc ggccggccct
     1801 gcgagccggc cgccccgtgc tgctaggcgg tttcgcagcc gagggacttc gcggtggcgt
     1861 tccatcgggc gagctggttg gcgcacgccg acgcgccctg gcccacctgg acctgcccgt
     1921 tgtccacggg tcccacgtat ctcccggtcg agcggagctt cgaggtcttc ttggtgatcc
     1981 cggccttcca gcggagggtc tccttcttga acttcgtgag ggcggcgatc tgcgtctgct
     2041 gccgcgcgtt ctccgtacgg agctcggcga tggcgtgggc ctgacggtcg gcacggtcct
     2101 cgaggtcggc gacccgttgg gacagcgtcg cgccaccgcg aggggtttcg gcccccgcgt
     2161 ccatggccac gacggcgaga ccgaggctcg cgatcaccgc gagcagcacg acgttcttgc
     2221 gcatccggaa ccctcctcca catccgacga ctccccggtc ggactcggcg tccgacgtcc
     2281 gacctgggtc gcgagcagcg tagaccggag cttcgctgct cctcgggaca tcctcggggt
     2341 ttcctctccc gcgattcggg taacctgctc gaccgacagc aaagaacgta ggtcgacgac
     2401 ggaggggaat gcatgaaggg gcgcatcgtg gtcgtgttgg gcttggtcat cgcggcggtg
     2461 tcggcgacgt cggcatccgc gggcgaactg gtggcttggg attcgttctc ggacggcgag
     2521 gcaaccatct cggattccgg cggcgcgtgg acgcaggtca accactcctg gtcggtgcag
     2581 gacgggcagg cagtggccgc ggccgatcgg acgcgcatcg gatacgcggt catggccacc
     2641 ccggtgtcag acgacttcac ggtgcaggcc gacatcacgt tgtcgcccac cgaccttcgg
     2701 gcggcccctg cgttggtcac gaactgggtc gacaacaaga acaacatggt gaccaaactc
     2761 gaagtcaccc ctggtcaccc cggcggcatg ctcgccgtcg gtgatcagat caacggacag
     2821 atccgctcga cgctctgcaa ggtggaacgg ctcgggctcg tgaacggcgt ggcttaccgc
     2881 ttgagcgtga cgcgggcggg cgacatcgtg accggctccg tgacctcgct cgaggacggc
     2941 tcggtgatcg gctcgtgctc ggcgatgctg tcctcggtgg cgtcgtcggc cttcggcgcc
     3001 gggaccggcc aggggctccg cgtcaagatc aaggacgacg aggacgacgg cggctcacgc
     3061 tgggacaact tcgcggtctt cacgaactga cgccgccggg ctacggtgcg ttgatgagcg
     3121 gttcggcgat gaaggatgcc ttcggacatc acgtgtgggc gacgacgcgg ttgatcgacg
     3181 tctgcatcgg attgccgccc gaacagctcg ccaccgccgt gcccggcacc tacggttcca
     3241 tcctcgacac gcagcgtcac ctcgtgggct cggacgcctg gtacctgcgt tgcatcacca
     3301 acgacgcgaa ggccgccatc gacgaggatc acatggatct gcgggagctc cggtccgcca
     3361 tggagcggaa cggcgcccgt tggtcccagc tcctcgccga tgagctcgac ccggacaccg
     3421 tcatcaccca ggtggatcac gaggacggct atcagaagga cgcgacggtc ggcatccggt
     3481 tcgcgcaggc gctgcatcac ggcaccgatc accgcagcca gatctgcacg gccctcacca
     3541 gcctcggcgt ggagccgcca ccgatcgacg tgtgggactt cggcgtcgag cacggtcgcg
     3601 tcgtcgaggt ctttcccacc tcctgacacg agcacagccc acgggatcgg aaccttcgct
     3661 aggctgctcg cccatgggga ccacgggagg gcgtcgccgg ctccgacgga cgctggacga
     3721 gaccgacgtc ctcatcatcg aggcgctgaa tcaggacggc cgacgctcgt tcgcggccct
     3781 cgcgcgggac ctcgatctga acgaggccac cgtccgatcg cggttcgccc gcctcacgcg
     3841 gcgcggcgtg gtgcaggtgg tggtgttggc cgacgcgctc gcgctcggtc tcgtgttcgc
     3901 cgaggtgggc atcaagatcc gcggcgggac ggtcaccagc gtcgtcaccg agctccagaa
     3961 ggtgcccgag atcgactaca tcgccgtctg tgcgggatcc ttcgacctgc tggtggagat
     4021 cgtgtgcgtc gacaatgagc acatgctgca ggtgctcgag gagcgcatcc ggccggtgga
     4081 cggcgtggat accgtcgaga cgttcacgat cctggacgtc ccgaagcaca gctaccggtg
     4141 gaccaagctg ttggcgtcca acgtgtcctt ccccgagtaa cgggttctgg ggtcacccag
     4201 cccttcgtca ttcggattcc atgaacaatc acgctctcta gcgccggatt ccgagccgtt
     4261 cgggtttgcc ccgccttcac ctccaaccta ggctgcgcag cctagaccga cgtgtcgagc
     4321 gctcgtgggg ggtcgatgaa cgagaacgaa gccgttgcct gggcgatcga ggaactcgtc
     4381 gcgcagatcg agatccggtc gatctcggac cacgaggacc ggatgatggc ctacctcgac
     4441 gaacggtgcc gggagctggg gttccccacc acgctccagc ccatcgaggg cgccggtccg
     4501 aacgtcgtgg tgcggtggga tggacggaac gatccgcccg acctgctcct gaccgcccac
     4561 accgacacca tcgacccggc gtgggactgg cgcgacgtcg ccacggtcga gggcaccgtg
     4621 gtcaccggac tcggcgcgca ggacgacaag gggtgcgggg tcgcgatcct gctcggcgcg
     4681 gtgctggcgc gtgaggccgg cgtcgatcta tccgagacct cggtcgcgct ggggttcgtc
     4741 gtcgacgagg aggtcggcgg caagggctcg cgggcgctcg ccgattcgct gcgcccccgg
     4801 ttcgtcgtcg cgtccgaggg gagcgagctg gacatcgcga ccgtggaaac cgggttcatc
     4861 gacggctggg tccgcgtgtc cggggtcacg atgcacggtt ccttgccgga ggaggccgac
     4921 aacgcggtcg agaaggcggc gcgactcctg ctcgcattgc acgaggcgcc gttcacgtcg
     4981 atctcccacc cgatcgccgg cccgaacacg gtcaccgcgc agcggatcga gagctcctcg
     5041 gcgatgaacg cgatccccga cgtggcgaac atgtacatca gctgtcgcgt gttcggcgaa
     5101 ccatcgctgg acgagacgcg gaaggagatc gaggcgatct gccgccggtt cggcgcgacc
     5161 ttcgagctcc acgaccacgg tggctggtgg gagaccccgc aggatgcccc gctggttcag
     5221 gcgatgatcc gcgcgagcga gcaggcgatc gggcgcacgc cgggcttcac gcggatgccc
     5281 gcctggaccg acgcccacag cttcttcgac cgatgcggat cggaggtcgt ggtcttcggc
     5341 cccggacatc tccgcgccgc acaccgaccc gacgaacacc tcgatgcccg tgaactcgtg
     5401 cgttgcgcgc gggtcttcgc ggcgttgatc gccgatacgg cgtccctgtc gacggcggaa
     5461 ccgacccgaa gcaaggaggg agttcgatga agagcccgtg gacacgctgc ttcgccctgg
     5521 tggtggcgct ggcactcacc gccgccgcat gcggcagcga cggggacgga gacgactcgg
     5581 cctcggctgc gggcggggag cagagcaccg gcccggtctc gggggagctg accttcttcg
     5641 cctacgagga tgcgttcgag ccggagctgc tggatccgtt cgaggcggcc aacccggatc
     5701 tcgacgtgcg caccgccgcg ttctcgagcg gtgacgagac ggtcacgaag ctccaaagcg
     5761 ggttccaggc cgacgtcgtc aacgtctgcg tcgaggacac gacacgcatg gtgaacctcg
     5821 gtctgctcca gccgatcgac acgggccgca tcgaggcgtg ggatgagatg ttccccgcgt
     5881 tcaccgggct gcccggcgtc gaggtggacg gtcagaccta cctggtgccg atggtcggtg
     5941 gcaccagcgg catcatgtac aacagccagg acatcccgga cgggttcgac tcctacgcca
     6001 gcgtgttcga tgaggcctac gccggccgga tcggtatgga cgacgacccg ctgtctggca
     6061 tcgccatcgg cgccatggcc gccgggatca ccgatccgat gaacctcgac gacgccgagc
     6121 tcgagcaggt gaagcagtac ctgatcgaga agaagccgct cctgcggagc ttcgtgaagg
     6181 gcgacgccga catcgcccag ctgttcaagt cgggcgagat cgacgtggtg gtgcccggct
     6241 acaagggctc gaccgagacc ctgcaacagg agggtgagcc ggtcgactac tccctggccg
     6301 atgaaggcca gctgacgtgg acctgcggct acgcgatcgg cgcgaacgca gcgaacgtcg
     6361 atgccgcgta cgcgttgatc aaccactatg cccagcccga aacgcaggcg tggcaggcgg
     6421 agaacttctt ctacctcgtg tcgaaccagg acacgctcga tgcggtgtcg cccaagatcg
     6481 tcgacgaagc ggggctggag gacccgggca gcttcgagca cgcgatcccc tacgcgatcc
     6541 cggagaacta cgacgcgtgg caggaagcat ggagggagat caaggccgcc tgatgagcgc
     6601 cgactcgacg aacgggcgtc cgcacgcggt gtgccgcatg tgcggtgagt ccatcacgag
     6661 tcggttcctc gatcgcgacg agctcgacgc a
//
LOCUS       DATDBZ010000110         2828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1878278, whole genome shotgun sequence.
ACCESSION   DATDBZ010000110 DATDBZ010000000
VERSION     DATDBZ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878278"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..521)
                     /locus_tag="VIX62_02715"
     CDS             complement(126..521)
                     /locus_tag="VIX62_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859182.1"
                     /translation="MALVGALALCLGTAACGSDSPDAGNADVKNEGSPTVPGARVIPV
                     DGINFAYEPSQITAAPGEQITIAMTSVEDRHDLVLLLGAESLLIAWASEGVTAVGGLT
                     APSEPGVYRFICSEIGHLTEGMEGQLIVE"
     gene            584..1756
                     /locus_tag="VIX62_02720"
     CDS             584..1756
                     /locus_tag="VIX62_02720"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY5859183.1"
                     /translation="MVQRVPHLTAKLQGFGTTIFAEMSSLAVATGSVNLGQGFPDYDG
                     PREVLDAAIDAINGGLNQYPPGAGMPVLREAISEHQRRFYNVDYDADTEVLVTAGATE
                     ALASVLLGLCDTGDEVVVLEPTYDSYQACIALAGASPRFVTLRPPDYHLDLDALRDAI
                     TAKTKLILLNTPHNPTGKVLTRAELSAVAELAVEHDLIVASDEVYEHLVFDGLEHVTL
                     ASLPGMRERTLVISSGGKTFNTTGWKVGWICGPPELVTAARTAKQFLTYVNSGPFQPA
                     IAVGLRLPDEYYAELAADLERKRDKLFAGLQDAGFDAFKPSGTYFITVDIRSMRADGD
                     GMEFCRSLPERCGIVAIPNVVFYDDKHEGRHMVRFAFCKRHEVIDDAVARLKGLAR"
     gene            1753..2514
                     /locus_tag="VIX62_02725"
     CDS             1753..2514
                     /locus_tag="VIX62_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HEY5859184.1"
                     /translation="MKVATIQHDIVWEDKEANFERLAPMIRSAAATGARIALLTETFS
                     TGFSMDTARTGEPVGGPSSAFLQQQAKEHDLWVGGSCPEVVGGDRPFNTFVLAGPDGT
                     VHRYRKVHPFTYGGEERSFQGGSDLVTVEIEGLRVSLFVCYDLRFADEWWQLARQTDV
                     YLIVANWPEARRVHWQALTLARAIENQAYVVACNRVGAGGGIDYAGDSRIIDPLGELL
                     ATGARTEALLVAEVDPDHVSAVRDRFRFLQDRRTS"
     gene            complement(2542..>2828)
                     /locus_tag="VIX62_02730"
     CDS             complement(2542..>2828)
                     /locus_tag="VIX62_02730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5859185.1"
                     /translation="LNYRWFAARYDAFEYLDRIVVAPTARGTGIGAAMYDELERRIAG
                     AAPWLLCEVNVRPLNEGSLRFHHRIGFVEVGQQDTDGGTKRVSLLAKPFR"
BASE COUNT          461 a          997 c          925 g          445 t
ORIGIN      
        1 ccagctcgcc atcgccgacg acgtcgacgt cgtgatcgag gaccgggaga cgctcgtgct
       61 catcttcgcc ggcgccgtgg cgccggcgac gaccgcggcc tgaccgcccg acaccggccg
      121 caccgtcact cgacgatgag ctggccctcc atgccttccg tcaggtggcc gatctcgcta
      181 cagatgaatc ggtagacacc cggctcgctc ggtgccgtga gtccgccgac ggcggtcacc
      241 ccttcgctgg cccaggcaat gaggagcgac tcggccccga ggagcaacac caggtcgtgg
      301 cggtcctcga ccgatgtcat cgcgatggtg atctgctcgc cgggcgcagc cgtgatctga
      361 ctcggttcgt aggcgaagtt gatcccgtcg acagggatga cgcgcgcgcc aggcaccgtc
      421 ggcgagccct cgttcttgac gtctgcgttg cctgcatccg ggctgtcgga cccgcatgcc
      481 gctgtcccga ggcacagcgc caatgcgccg accagtgcca ttcgccgtgc accatccatc
      541 cgaccatcct gtcaaacctg tcgcagacct acgattcgag tcgatggtcc agcgcgtccc
      601 gcacctcacc gccaagctgc agggcttcgg cacgacgatc ttcgccgaga tgagctcgct
      661 cgcggtggcg accggttcgg tgaacctcgg gcagggcttt cccgactacg acggaccgcg
      721 cgaggtgctc gacgccgcga tcgacgcgat caacggcgga ctcaaccaat acccgcccgg
      781 tgccggcatg ccggtgttac gcgaagcgat cagcgaacac caacggcgct tctacaacgt
      841 cgactacgac gccgacaccg aggtgctcgt caccgccggc gcgacggaag cgctggcatc
      901 ggtcctcctc ggcctgtgcg acaccggcga cgaggtggtc gtgctggagc cgacgtacga
      961 cagctaccag gcctgcattg cgctcgccgg cgcgagcccc cgcttcgtca cactgcgacc
     1021 acctgactac cacctcgacc tcgacgccct ccgcgacgcg atcacggcga agacgaagct
     1081 gatcctgctg aacaccccgc acaacccgac gggcaaggtg ctcacgcgtg ccgagctgtc
     1141 ggccgtggcg gagctcgccg tggagcacga tctgatcgtc gccagtgacg aggtctacga
     1201 gcacctcgtg ttcgacgggt tggagcacgt cacgctggcc tcgctccccg gcatgcgaga
     1261 gcgcaccctc gtcatctcat ccgggggcaa gacgttcaac accacgggct ggaaggtggg
     1321 ctggatctgt ggcccgcccg agctcgtcac ggcggcgcgc acggccaagc agttcctgac
     1381 ctacgtcaac agcggtccct tccagcccgc gatcgcggtg ggattgcggc tcccggacga
     1441 gtactacgcg gagctggcgg ctgacctgga gcggaagcgg gacaagctct tcgccggtct
     1501 gcaggacgcg ggcttcgacg cgttcaagcc ctcgggcacg tacttcatca ccgtcgacat
     1561 ccgctcgatg cgggccgacg gcgacggcat ggagttctgc cgatcgctgc ccgagcggtg
     1621 tggcatcgtc gccatcccca acgtggtctt ctacgacgac aagcacgaag gccgccacat
     1681 ggtgcggttc gcgttctgca aacgccacga ggtgatcgac gacgcggtgg cgcgcctgaa
     1741 ggggctggca cgatgaaggt cgcgaccatc cagcacgaca tcgtctggga ggacaaggaa
     1801 gcgaatttcg agcgcctggc accgatgatc cggagcgcag ccgccacggg cgcccgcatc
     1861 gccctgctca ccgagacgtt ctcgaccggc ttctcgatgg acaccgcccg taccggcgaa
     1921 ccggttggtg ggccgagctc tgcgttcctg cagcagcagg cgaaggagca cgacctctgg
     1981 gtcggcggtt cctgccccga agtggtcggc ggtgaccggc cgttcaacac cttcgtgctc
     2041 gccggtcccg acggcacggt gcaccggtac cgcaaggtcc accccttcac ctacggcggc
     2101 gaggaacgct cgttccaggg cggcagtgac ctcgtgacgg tcgagatcga aggacttcgg
     2161 gtcagcttgt tcgtctgcta cgacctccgg ttcgccgacg agtggtggca gctcgcccgt
     2221 cagaccgacg tgtatctcat cgtcgcgaac tggcccgagg cgcgccgggt gcactggcag
     2281 gcgctgacgc tggcccgggc gatcgagaac caggcgtacg tggtggcctg caaccgggtg
     2341 ggggcaggcg gcgggatcga ctacgcgggc gacagccgga tcatcgaccc gctcggtgag
     2401 ctcctggcga ccggcgcccg caccgaagcg ctcctggtgg ccgaggtcga tcccgaccac
     2461 gtgagcgcag ttcgcgatcg cttccgattc ctgcaggacc gccgcacatc ttgatttgcg
     2521 cctgcgcgcc tgctgatccg gtcagcggaa gggtttggcg aggaggctca cccgcttggt
     2581 cccgccgtcc gtgtcctgct gaccgacctc gacgaagccg atgcggtggt ggaagcgcag
     2641 cgagccctcg ttcaacggcc gcacgttcac ctcgcacagc agccacggcg cggcgccggc
     2701 gatccgccgt tcgagctcgt cgtacatcgc agcaccgatg ccggtgccgc gcgccgtcgg
     2761 cgccaccacg atccgatcca ggtactcgaa cgcgtcgtag cgcgccgcga accaccggta
     2821 gttgaggc
//
LOCUS       DATDBZ010000111         7652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1043787, whole genome shotgun sequence.
ACCESSION   DATDBZ010000111 DATDBZ010000000
VERSION     DATDBZ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043787"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..471)
                     /locus_tag="VIX62_02735"
     CDS             complement(52..471)
                     /locus_tag="VIX62_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY5859186.1"
                     /translation="MPRSDDSPASKRPAKGSAASSTTKSRKARSKKSGAGDEVRLTGD
                     RLVVRSTDGAERTSTGGLLIPATAAPAPKRLSWADVVLVGPDVRVASKGDRVLFLPSS
                     ALEVELEGEDLILLRERDVQAVTAADDKAERSPGQYL"
     gene            513..1373
                     /locus_tag="VIX62_02740"
     CDS             513..1373
                     /locus_tag="VIX62_02740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEY5859187.1"
                     /translation="MEAKRVLSRAVKTLKASDSIDHWQKDRERIEAEDLLIHVMGYEP
                     EPDEDIAGSDRREFEALIERRATGEPIPYIKGYAEFRGIELRTKPGVFVPRDSSEFLA
                     EQAVRRLRGRKKPVHVDLATGGGTIALAVADEVPKAQVWGTDVAADAVKLARKNAKML
                     DLTATFKRGDLFGALPKSLRGTVDVITLHPPYVPRDEIEDLPDEIRDWEPEHTLTDRS
                     IDGMGLIHRTVEESPAWLRKGGWLLMEVSPDRAKGVKKVFRHGGFDEVESTKGGELKV
                     TRVIVGRRPR"
     gene            1370..3337
                     /locus_tag="VIX62_02745"
     CDS             1370..3337
                     /locus_tag="VIX62_02745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5859188.1"
                     /translation="MTEPTTAPFGSWSSPITASMLSSAGMGLSEVWLEDGVAYWLETR
                     PSEQGRGIVMRGDPWSSPTEVTPEGFNVRTTVHEYGGGAFAVHRGTVVFSHRVDQRLY
                     RLGTNDATPVPISPDTSGTHRYADGRITADGRRWIGVRERHEGAGEPAEVVNELVVLP
                     LDGDAPPRAVATGRDFYSDPRVSPDGLRLAFLAWDLPWMPWDGCELFVGDLGDDGSVS
                     NLRLVAGADGEESIWAPDWSPAGVLHFASDRTGWWNLERIVDGERQSLCPMEAEFGFP
                     HWVFGSRSYGFLEDGRIACWYERSGTQMLAVLDPETGELLDLDLPHTALHWGPAMAVE
                     GSQIAFVAGAPDRPEELVWLDFTARSVEVLRVSAEMPFDPAFVSVPSRIEFPTDGGVT
                     AFANVYEPANPGFAGPAGERPPLIVMSHGGPTGEATAVFDLEMQFWTSRGFAVVDVNY
                     GGSTGFGRAYRRRLNGMWGITDTADCINAARHLANEGVVDPDRLLIRGGSAGGYTTLC
                     ALTFHDGFAAGTSYYGISDLEPFATGETHKFESRYLHTLVGPWPQSADLYRARSPIHA
                     VGMISTPMLVLQGADDEVVPPSQSEVMVEALRVKGLPHAYLLFEDEQHGFRKAETIQA
                     ALEAELSFYAQILGFEPGDPIPALTIVNLDR"
     gene            3580..5454
                     /locus_tag="VIX62_02750"
     CDS             3580..5454
                     /locus_tag="VIX62_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5859189.1"
                     /translation="MKGSARWIALVTILGVVAAACSGNSGDTDPSAGSSVEPAKGGTL
                     HIGVSSEPASAAMDPAKEYYSLSWEQMRCCLLRTLYSTNGQALAQGGLDLRPDLAASL
                     PEISEDGLTYTFAIRRGVKFAPPFQKEEITADDFIRALEREATPAASSGGYSFYYSPI
                     VGFDDFGAGKANEIAGLKAIDDYTLEMTLSEPTGDLGWRMAMPATAPIPPNGNAPLGA
                     AEGHNADYGRFMVASGPYMFEGSEDLDFSVPADDQEPVAGYTPGRSITMVRNPSWDAS
                     TDDLRAAYVDSIEIQIGGAVADLYDKVQTGDLDYVFDPPEAAALKQYSTDPSLKDRLH
                     VNPTSGTSYASMNLGAPPFDDIYVRKAMNLIWDKTGGRQLGGGPLTGVNAGHIFPNSL
                     LNNVLADYNPYATPNDSGDIGLAQEQMKQSKYDTDQDGICDAPECDDVLAIYASDSAT
                     GRKIAALTQQSLEEIGITLDVKGLSTTPMYAKCNDMTEQVPVCLQLSWGQDYPDPYTF
                     GPPLFGGSAFGALYPGCCNYAGVGATEAEMTEWGYSVTSVPSVDDQLNACKALAVGDE
                     RTQCWADLDRYLMEDVVPWVPKTFLNQIDILSARVTSYSFDEFGGLAALDRLAVEAAA
                     "
     gene            complement(5476..6507)
                     /gene="moaA"
                     /locus_tag="VIX62_02755"
     CDS             complement(5476..6507)
                     /gene="moaA"
                     /locus_tag="VIX62_02755"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY5859190.1"
                     /translation="MTTLPHASDAGSAPAAGPLIDTFGRVASDLRISVTDRCNFRCTY
                     CMPAEGVAWLPKDEILTFEELTRLLALFVSLGVDSIKVTGGEPTVRADLPTLVGMFRG
                     VGPELDISITTNGVLLDTLAEPLARAGVDRATVSCDSLLRHRFAEMTRRDALDKVLRG
                     LQAAEAAGLTPIKINCVVIGGTNDDEVAGFARWARETGYEVRFIEYMPLDAQHAWERE
                     KVVPAATMLERIGAVYPLVASTRDGEPATSFRFADGAPGGIGVIPSVTEPFCDSCNRM
                     RLTAEGQFRNCLFALDETDLRGPMREGADDAQLEALIRDAMWRKWSGHRINHPDFQQP
                     ARSMSMIGG"
     gene            complement(6548..6805)
                     /locus_tag="VIX62_02760"
     CDS             complement(6548..6805)
                     /locus_tag="VIX62_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HEY5859191.1"
                     /translation="MSTWTETLASALGVPPLSEEESERLLDASRDVAHRVERKDTPLT
                     TFLVGAAVGRATAGGLEPGAAIDDALATLARMLPAGAPGDP"
     gene            6872..>7652
                     /locus_tag="VIX62_02765"
     CDS             6872..>7652
                     /locus_tag="VIX62_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY5859192.1"
                     /translation="MDPRPRAAVVTISDGVTHGTREDASGDLASELVAAAGFEVVERV
                     VVPDERDRIEDTIRTLAAMHAVVITTGGTGFGPRDVTPEATRAVLDREAPGLAELMRE
                     AGRAHTAMAALSRGVVGTVGSALVVNLPGSPAGVREGLEVVLPLVPHAIGLMQGDTGA
                     HPTGHPSGRQEAPEPDAPTDELRVDAKAVKVHGAPPCQVGNAMTIVPGGAVHGTLGCA
                     EFDMAAVEAATEVAASGRAETRTLTHDLGQVEVYFEPAVTAR"
BASE COUNT         1230 a         2551 c         2702 g         1169 t
ORIGIN      
        1 gagaacgacg tccggtcggt gcggctgtcg ttcctggcgg gctgagccgg cctagaggta
       61 ctggcccggg gaacgctcgg ccttgtcgtc ggcggccgtg accgcctgca cgtcgcgctc
      121 gcgcaacagg atcaggtcct cgccctcgag ttcgacctcg agcgccgacg acggcaggaa
      181 cagcacccgg tcgcccttcg aggcgacccg gacgtccgga ccgaccagca ccacgtccgc
      241 ccatgacagg cgtttgggcg cgggtgccgc agtggccggg atcagcagcc cgccggtcga
      301 tgtgcgttcc gcgccgtcgg tcgatcgcac gaccaggcga tcgcccgtga gccgaacctc
      361 gtccccggcc cccgacttct tcgaccgggc cttgcgcgac ttggtggtcg acgaagccgc
      421 cgaccccttg gcgggccgct tcgatgcggg cgagtcgtcg ctgcgaggca tcgcccgagt
      481 ctatcgaccg ccaccgggta cgatgcctcg ccatggaagc caagcgagtg ctctcgcgcg
      541 cggtgaagac gttgaaggct tcggattcga tcgaccattg gcagaaggac cgcgagcgca
      601 tcgaggccga ggatctcctg atccacgtca tggggtatga acccgaaccc gacgaggaca
      661 tcgccggcag cgaccggcgt gagttcgagg cgttgatcga acgccgtgcc accggcgagc
      721 cgatccccta catcaagggc tacgcggagt tccgaggtat cgagctgcgt accaagcctg
      781 gggtcttcgt gccgagggac tcgtcggagt tcctcgccga gcaggcggtg cgccggttgc
      841 gcggccgcaa gaagccggtg cacgtcgatc tggcgaccgg cggcggcacg atcgccctcg
      901 cggttgccga cgaggtgccg aaggcgcagg tgtgggggac cgacgtggcg gccgacgcgg
      961 tcaagctcgc gcggaagaac gccaagatgc tcgacctcac cgcgaccttc aaacggggcg
     1021 acctgttcgg cgcgctgccg aagtcccttc gaggcacggt cgacgtcatc accctccacc
     1081 ctccgtacgt gccgcgcgac gagatcgagg atctgcccga tgagatccgc gactgggaac
     1141 ccgagcacac cctcaccgac cggagcatcg acgggatggg tctgatccat cgcacggtcg
     1201 aggagtctcc tgcgtggctc cggaagggcg gatggctcct gatggaggtc agcccggacc
     1261 gggccaaggg tgtgaagaag gtgttccgcc acggggggtt cgacgaggtc gagagcacga
     1321 agggtggcga actcaaagtg acgcgggtca tcgtcgggcg ccggccgaga tgaccgaacc
     1381 cacgacggcg ccgttcggct cgtggtcctc accgatcacg gcctcgatgc tctcgagcgc
     1441 cggcatgggc ctcagcgagg tgtggttgga agacggtgtc gcgtactggc tcgagacccg
     1501 gccgtccgag cagggccgag gcatcgtgat gcgcggcgac ccctggtcct cgcccaccga
     1561 ggtcaccccc gaagggttca acgtgcgcac caccgtgcac gagtacgggg gtggagcctt
     1621 cgcggtgcat cgcggcaccg tcgtgttctc gcatcgcgtc gaccagcggc tctaccggct
     1681 cgggacgaac gacgccacgc cggtgccgat ctctcccgac acgtccggga cgcatcgcta
     1741 cgccgacggg cgcatcaccg ccgatgggcg ccggtggatc ggcgtgcggg agcgccatga
     1801 aggtgccggt gagccggccg aggtggtgaa cgagctcgtc gtgctgccgc tcgacggcga
     1861 tgcccccccg agggcggtcg ccacgggccg agacttctat tcggaccccc gcgtctcgcc
     1921 cgacggcttg cgcctggcgt tcctggcctg ggacctgccg tggatgccgt gggacggctg
     1981 tgagctgttc gtcggtgacc tcggcgacga cggttcggtg tcgaaccttc gcctcgtggc
     2041 cggcgccgac ggcgaggaat cgatctgggc gcccgactgg agcccggcgg gggtgctgca
     2101 cttcgccagt gatcggacgg gttggtggaa cctggaacgc atcgtggacg gcgagcggca
     2161 gtccttgtgt cccatggagg ccgagttcgg gttcccgcac tgggtgttcg gctcccggtc
     2221 ttacgggttc ttggaggacg ggcggatcgc ctgttggtac gaacggagcg gcacccagat
     2281 gctggcggtg ctcgatccgg agaccggcga gctgctggac ctcgacctgc cccacaccgc
     2341 cctgcattgg ggccccgcga tggccgtgga ggggtcccag atcgcgttcg tggccggtgc
     2401 ccccgaccgc cccgaggaac tcgtctggct ggacttcacc gcgcgatccg tcgaggtgct
     2461 gcgcgtcagc gcggagatgc cgttcgatcc ggcgttcgtg tcggtgccgt cccggatcga
     2521 gttcccgacg gacggcgggg tcacggcgtt cgccaacgtg tacgaaccgg cgaatccggg
     2581 gttcgccggt cccgccgggg agcgcccgcc gctgatcgtg atgagccacg gaggaccgac
     2641 gggtgaagcc accgccgtgt tcgacctgga gatgcagttc tggaccagtc gcgggttcgc
     2701 cgtggtcgac gtgaactacg gcggcagcac cggcttcggc cgagcgtacc ggcgccggct
     2761 caacgggatg tggggcatca ccgacacggc agactgcatc aacgccgctc ggcatctcgc
     2821 gaacgagggc gtcgtcgacc cggaccggtt gctgattcgc ggggggagcg cgggggggta
     2881 cacgacgctg tgcgcgctga ccttccacga cgggttcgcc gccggcacca gctactacgg
     2941 catcagcgac ctcgagccgt tcgcgacggg tgagacccac aagttcgagt cccgctacct
     3001 gcacacgctg gtcggaccct ggccgcagag cgccgacctc taccgagcgc gcagccccat
     3061 ccacgccgtg ggcatgatct cgaccccgat gctcgtgtta cagggagccg acgacgaggt
     3121 ggtcccgccg tcgcaatccg aggtgatggt ggaagccttg cgggtcaagg gtctgccgca
     3181 tgcctacctc ctgttcgagg acgagcagca cgggttccgc aaggccgaaa ccatccaggc
     3241 cgccctcgag gcggaactgt ccttctacgc ccagatcctc gggttcgaac ccggcgaccc
     3301 catcccggca ctgacgatcg tcaacctgga tcggtgaggg gggttcgcgg gaggccggga
     3361 cgtgcccggc cccccgcgcc gaagaggggg cgcatcctct cggtgcacac agcctcccgg
     3421 ccccggatga acgccgcggg aaccgagtgg gaccgacggg cgaacgatcg cggtcgggcc
     3481 gggcttcgcc tcgggacaac ctcccggcac gaggcgctgc gtataccgtg gtgatcgcag
     3541 tcgaaggcat cggatgcaga cgaagagggg gtttcgcgca tgaagggatc cgcacgctgg
     3601 atcgccttgg tgacgatcct gggggtggtc gccgcagcgt gtagcggcaa cagcggcgat
     3661 acggatccca gcgcgggcag cagcgtggag cccgccaagg gcggaaccct ccatatcggg
     3721 gtatcgagcg aaccggcgag tgccgccatg gaccccgcga aggagtacta ctccctctcc
     3781 tgggagcaga tgcggtgttg cctgctcagg accctctact cgacgaacgg ccaggccctg
     3841 gcccagggcg gattggatct gcgcccggac ctggcggctt ccctcccgga gatctccgag
     3901 gacggcttga cctacacgtt cgcgattagg cggggcgtga agttcgcacc ccccttccag
     3961 aaggaagaga tcacagccga cgacttcatc cgggcgttgg agcgtgaggc gaccccggcg
     4021 gcctcatcag ggggatactc cttctattac tcaccgatcg tgggattcga cgacttcggc
     4081 gccgggaaag cgaacgagat cgccggcctg aaggccatcg acgactacac cctcgagatg
     4141 acgctgtccg agccgaccgg agatctcgga tggcgtatgg cgatgcccgc cacggcgccg
     4201 atcccaccga acgggaacgc tccgttgggc gcggccgagg ggcacaacgc ggactacgga
     4261 cggttcatgg tcgcatcggg gccctacatg ttcgagggca gcgaggacct ggacttctcc
     4321 gtgccggccg acgaccaaga gcccgtggcc ggctacaccc ccgggcgctc gatcacgatg
     4381 gtgcgcaacc cgtcgtggga tgcgtccacc gacgatctcc gggccgccta cgtcgactcg
     4441 atcgagatcc agatcggtgg ggccgtcgcc gacctgtacg acaaggtgca gacgggcgac
     4501 ctcgactacg tgttcgatcc gccggaagcg gcggccttga agcagtacag cacggacccg
     4561 agcctcaagg atcggctgca cgtcaatccc accagcggga cgtcgtacgc gtcgatgaac
     4621 ctgggcgcgc cgcccttcga cgacatctac gtgcgcaaag ccatgaacct catctgggac
     4681 aagacgggcg gacggcagct cggaggagga ccgctcaccg gagtcaacgc cgggcacatc
     4741 ttccccaata gcttgctgaa caacgtgctc gcggactaca acccgtacgc gacgccgaac
     4801 gacagcggcg acatcgggtt ggctcaggaa cagatgaagc agtcgaaata cgacacggac
     4861 caggacggca tctgcgatgc acccgagtgc gacgacgtgc tcgcgatcta tgcctccgac
     4921 agcgccaccg gccgcaagat cgccgcgctg acgcagcaat cactcgagga gatcgggatc
     4981 acgctcgatg tgaaggggtt gtccacgacg ccgatgtacg ccaagtgcaa cgacatgacc
     5041 gagcaggtgc cggtgtgtct gcagctctcg tggggccagg actatcccga cccgtatacg
     5101 ttcgggcctc cgttgttcgg cggctctgcg ttcggtgccc tgtatcccgg ctgctgcaac
     5161 tacgcagggg tgggcgccac ggaggccgag atgacggagt ggggctactc ggtgacgtcc
     5221 gtgccgagcg tcgatgacca gttgaacgcc tgcaaggccc tggccgtcgg tgacgagcgc
     5281 acccaatgtt gggccgacct cgacaggtac ctgatggaag acgtcgttcc gtgggtgccg
     5341 aagaccttcc tcaaccagat cgacatcctg agtgctcgag tgacgagcta ctcgttcgac
     5401 gagttcggtg gcctggcggc gctcgaccgc ttggcggtgg aggcagccgc gtagggctgc
     5461 ctcgatccga tcggcctagc cgccgatcat cgacatgctg cgcgccggct gctggaagtc
     5521 cgggtggttg atccggtggc ccgaccattt gcgccacatc gcgtcgcgga tcagtgcctc
     5581 gagttgcgcg tcgtcggcgc cctcacgcat cggcccgcgc aggtcggtct cgtcgagcgc
     5641 gaacaagcag ttgcggaact gcccctcggc ggtcaagcgc atgcggttgc acgagtcgca
     5701 gaacggttcg gtgacgctgg ggatcacgcc gatcccgccg ggcgcgccgt cggcgaagcg
     5761 gaacgaggtg gccggttccc cgtcgcgcgt ggaggcgacc aacggataga cagcgccgat
     5821 ccgttcgagc atcgtggcgg cggggaccac cttctcgcgt tcccaggcgt gctgagcgtc
     5881 gagcggcatg tactcgatga agcgaacctc gtagccggtc tcacgcgccc aacgggcgaa
     5941 cccggcgacc tcgtcgtcgt tcgtcccgcc gatcacgacg cagttgatct tgatcggtgt
     6001 cagccccgcg gcttcggcgg cctgtagtcc ccgcagcacc ttgtccaggg cgtcgcgtcg
     6061 ggtcatctcg gcgaaccgat ggcgcaagag cgagtcgcac gagaccgtgg cccgatcgac
     6121 gccggcgcgc gcgagcggct cggccaacgt gtccaggagc acgccgttcg tcgtgatcga
     6181 gatgtcgagc tcaggtccca cccctcggaa catgcccacc agggtgggga ggtcggctcg
     6241 gacggtcggc tcgccgcccg tcaccttgat cgaatcgacg cccagcgaga cgaagagtgc
     6301 cagcaacctc gtgagttctt cgaacgtgag gatctcatcc ttgggaagcc acgcaacacc
     6361 ctcggccggc atgcagtagg tgcaccggaa gttacagcgg tcggtgaccg agatccgcag
     6421 atccgaggcc acgcgcccga acgtgtcgat cagcggcccc gctgcgggag ccgagccggc
     6481 gtcggaagcg tgaggaagcg tggtcatggt ggaccgccag cgtaccttcg ttcgagggaa
     6541 gggcgtgtca cgggtcgcct ggagccccgg ccggcagcat ccgtgccagg gtggccaggg
     6601 cgtcgtcgat cgccgcaccg ggttcgagcc cgcccgccgt ggcccgtcct accgccgctc
     6661 ccaccaggaa ggtggtcagc ggagtgtcct tgcgctccac acgatgcgca acatcgcggg
     6721 aggcgtcgag caggcgctcg gattcctcct cgctcagcgg tgggacgccc aacgccgacg
     6781 ccagggtctc ggtccacgtg ctcatgtcgt gcctcctcgt cgtgagatcc gcgcgcgggt
     6841 cgcccggcgc cggggatgcg aggatggtgc catggaccca cggcctcgcg cagccgtcgt
     6901 cacgatctcc gacggcgtga cccacggtac ccgtgaggat gcctcgggcg atctggcgtc
     6961 cgaactggtg gccgccgcag ggttcgaggt cgtggagcgg gtggtggtcc ccgacgaacg
     7021 agatcggatc gaggacacca tccgaaccct cgccgcgatg cacgccgtgg tgatcaccac
     7081 cggggggacc gggttcggcc ctcgtgacgt cacgccggag gccacccgtg cggtgctgga
     7141 ccgcgaggcc ccggggctgg ccgagttgat gcgcgaggcg ggtcgagcgc acaccgcgat
     7201 ggcggcgctg tcccgcgggg tggtcggcac ggtcggctcg gccctggtgg tgaacctgcc
     7261 cgggtcgccg gccggggtgc gtgagggcct ggaggtcgtc ctgcccctgg tgccccacgc
     7321 gatcgggctg atgcagggcg ataccggggc acaccccacc gggcatccct ccgggcgtca
     7381 ggaggctccg gaaccggacg cgccgacgga tgagcttcgc gtggacgcga aggcggtgaa
     7441 ggtgcacggc gcgccgccgt gccaggtcgg caacgcgatg acgatcgtgc ccggtggcgc
     7501 cgtccatgga accctcggct gcgccgagtt cgacatggcg gcggtcgagg ctgccaccga
     7561 ggttgccgcc tcgggacggg ccgagactcg aaccctcacc cacgacctgg ggcaggtcga
     7621 ggtctacttc gagccggcgg tgacggcgcg gc
//
LOCUS       DATDBZ010000112         2569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2291879, whole genome shotgun sequence.
ACCESSION   DATDBZ010000112 DATDBZ010000000
VERSION     DATDBZ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291879"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VIX62_02770"
     CDS             <1..969
                     /locus_tag="VIX62_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY5859193.1"
                     /translation="FVEAMSKTFGVLHSDRVALALSPFVYFLGRLFAWPTRGLIGLAN
                     VLLPGKGIKAGPFVSEEEIRSMAQVGSEEGSIDVGEKELIHSIFEFGDTIVREVMVPR
                     PDIIAIEDDKSLRDVQALVLTHGTSRIPVYDADEDLDHVVGIVFAKDVLKSLHQGKHD
                     APLAEVVREAHYVPESKKVADLLREMQREKFHMALVTDEYGSVTGLISLEDLLEELVG
                     EITDEYDREEPEFEQVGENIYRVSGKTSIDDINELLDVELPDEEWDTVAGLVLDCFGR
                     IPDAGEECQLDGLTFRAETVVGRRIASVLITRTTEPEPVLTEVPSP"
     gene            966..1379
                     /locus_tag="VIX62_02775"
     CDS             966..1379
                     /locus_tag="VIX62_02775"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY5859194.1"
                     /translation="MTSIDDARLEEMLAAAREVRARAYVPYSGFHVGAALLAGDEIFT
                     AVNVENASYPLSVCAERNAIAKAIAAGSTDIRAVVVVSDADTPTPPCGGCRQVLREFG
                     NGIAVIAESADGSKRTFWSIDELLPHAFGPDDFQP"
     gene            1376..2263
                     /gene="era"
                     /locus_tag="VIX62_02780"
     CDS             1376..2263
                     /gene="era"
                     /locus_tag="VIX62_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY5859195.1"
                     /translation="MTGFRSGVVAVVGRPNVGKSTLVNRLVGEKVAIVSDKPQTTRRD
                     IRAIWTTDEAQVVFTDTPGFHKPRTLLGARLNDLVGDAVTGVDVIVLVVDGHMGVGRG
                     DAFVYAKQVAGEAEATKICVVNKIDRLAHTKVVPQLAAAQALGDWDEIVPVSAAVGDG
                     VEELRAVLLSHLPEGPALFPDGEITDQPLEVRLTELVREQALAVTREEVPHSVAVVID
                     EMDREGNLTKVHASIVVERDSQKGILIGHGGQTLKTIGTKAREQMEPLLGTKVYLDLH
                     VKVMKEWQRDPKALDRLGF"
     gene            complement(2271..>2569)
                     /locus_tag="VIX62_02785"
     CDS             complement(2271..>2569)
                     /locus_tag="VIX62_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY5859196.1"
                     /translation="EAKAVDLTDDEAAELEIEDFAYEPACATIAKGQALEIRNRDNFS
                     HTFTVDALDITIPIDGDATITMANRGKEPKLAVGTNPFHCSIHPEMTGTIFVTG"
BASE COUNT          432 a          828 c          900 g          409 t
ORIGIN      
        1 ttcgtcgagg cgatgagcaa gacgttcggc gtgctgcaca gcgatcgcgt cgcgctggcg
       61 ctctcgccct tcgtgtactt cctgggacgg ctgttcgcct ggccgacccg cggcctgatc
      121 ggtctggcga acgtgctgct gccgggcaag ggcatcaagg ccggtccgtt cgtctcggaa
      181 gaagagatcc gctcgatggc gcaggtcggc tctgaggaag gctcgatcga cgtcggcgag
      241 aaggaactga tccactcgat cttcgagttc ggcgacacga tcgtgcgcga ggtcatggtg
      301 ccgcgtcccg acatcatcgc catcgaggac gacaagtcgt tgcgcgatgt ccaggcactg
      361 gtgctgacgc acggcacgtc caggatcccc gtctacgacg ccgacgaaga cctcgaccac
      421 gtcgtcggga tcgtgttcgc caaggacgtg ctgaagtcgc tgcaccaggg caagcacgac
      481 gcgccgctcg ccgaggtcgt ccgcgaggcg cactacgtgc cggaatcgaa gaaggtcgcc
      541 gacctgttgc gcgagatgca gcgagagaag ttccacatgg cgctcgtcac cgacgagtac
      601 ggctcggtga ccggcctgat atccctcgaa gacctcctgg aagaactcgt gggggagatc
      661 accgacgagt acgaccgcga ggagcccgag ttcgagcagg tgggcgagaa catctaccgc
      721 gtcagtggga agacctcgat cgacgacatc aacgagctgc tcgatgtcga gctgcccgac
      781 gaggagtggg atacggtcgc cggcctggtg ctcgactgct tcgggcgcat ccccgacgcc
      841 ggcgaggagt gccagctgga cgggctcacc ttccgcgccg agaccgtcgt gggtcgccgc
      901 atcgcgagcg tcctcatcac caggaccacg gagcccgagc cggtgctcac cgaggtaccg
      961 tcgccatgac gtcgatcgac gacgctcgcc tcgaggagat gctggcggcg gcgcgcgagg
     1021 tccgcgcccg cgcctacgtc ccgtacagcg gcttccacgt gggcgccgcc ctgctggcgg
     1081 gcgacgagat cttcaccgcg gtcaacgtcg agaacgcgag ctacccgctc agcgtgtgcg
     1141 ccgagcggaa cgcgatcgcc aaggcgatcg cggccggcag caccgacatc cgggcggtgg
     1201 tggtggtctc ggacgccgac acccccacac cgccgtgcgg gggatgccgc caggtcctgc
     1261 gtgagttcgg gaacgggatc gccgtgatcg ccgagtcggc ggacggttcg aagcgcacct
     1321 tctggtcgat cgacgaactg ctgccgcacg ccttcggccc ggacgacttc caaccgtgac
     1381 cggcttccgg tccggggtgg tcgcggtggt gggccggccc aacgtcggca agtccaccct
     1441 ggtcaaccgg ctggtggggg agaaggtcgc gatcgtctcg gacaagcccc agaccacgcg
     1501 ccgggacatc cgtgccatct ggaccaccga cgaggcccag gtcgtcttca ccgacacgcc
     1561 ggggttccac aaaccgcgga cgctgctggg ggctcgcttg aacgacctcg tgggcgacgc
     1621 cgtgaccggc gtcgacgtga tcgtcctggt ggtcgacggg cacatgggcg tcgggcgcgg
     1681 cgacgcattc gtctacgcga agcaggtggc gggcgaagcg gaggccacga agatctgtgt
     1741 ggtcaacaag atcgaccggc tcgcccacac caaagtggtg ccgcagctgg cggccgccca
     1801 ggcgttgggg gactgggacg agatcgtgcc ggtctcggcc gcggtcggtg acggcgtcga
     1861 ggaattgcgg gcggtgctgc tctcgcacct gccggaagga cctgccctct tccccgacgg
     1921 cgagatcacc gatcagcccc tcgaggtccg cctcaccgag ctcgtccggg agcaggccct
     1981 cgcggtgacc cgcgaggaag tgccccattc ggtggcggtg gtgatcgacg agatggatcg
     2041 cgagggcaac ctcacgaagg tgcatgcctc gatcgtggtc gagcgcgatt cgcagaaggg
     2101 gatcctgatc ggtcacgggg gccagacctt gaagacgatc ggcaccaaag cgcgggagca
     2161 gatggagccg ctcctgggca ccaaggtcta cctcgacctg cacgtcaagg tcatgaagga
     2221 atggcagcgc gacccgaaag cgctcgaccg gctggggttc tagacgcgag ctagcccgtg
     2281 acgaagatcg tgccggtcat ctccgggtgg atcgagcagt ggaacgggtt cgtgcccacc
     2341 gccagcttcg gctccttgcc ccggttcgcc atggtgatcg tggcgtcacc gtcgatgggg
     2401 atcgtgatgt ccaaggcgtc cacggtgaac gtgtgcgaga agttgtcgcg gttgcggatc
     2461 tccagggcct ggcccttcgc gatcgtggcg cacgccggct cgtaggcgaa gtcctcgatc
     2521 tccagttccg cggcttcgtc gtcggtgaga tcgacagcct tggcttccg
//
LOCUS       DATDBZ010000113         2634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1266016, whole genome shotgun sequence.
ACCESSION   DATDBZ010000113 DATDBZ010000000
VERSION     DATDBZ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266016"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VIX62_02790"
     CDS             <1..918
                     /locus_tag="VIX62_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5859197.1"
                     /translation="GPTAVAWVLPALGLCSASLMLATFVPLRRAALLVTGSWLVLAAG
                     VGLMGPAASLFQGSAQLLFFAVTVICSLVLSRRRHHLEIANLHSRRALVDAADNERRR
                     IERNIHDGAQQQLVAIGVKANLARAMVANDPDKAVEIIDQVCADAKAALAALRDMTRG
                     AYPPILADEGLPAALAAKAAAAPLAVTLDAGGVGRLPKELEIATYFCCTEAIQNAVKH
                     AKASSIEVTLRPRVGAFTFSVVDDGEGFEPSTMRRGVGMRSMVERVESLGGSLDVRSR
                     VGKGTTISATIPLHPDRRAPQANADVTTV"
     gene            982..2046
                     /locus_tag="VIX62_02795"
     CDS             982..2046
                     /locus_tag="VIX62_02795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5859198.1"
                     /translation="MPTRPDVEIVGASFAGLACAQACAARGLDAVVWERKRDAGAAVR
                     TTGILVKEAADELDPPVELTRTLHAVRLYSPSLDWVDLDAPGYRFLATDTPELLRALA
                     RRAQDVGADIRWGSPFRPPRLAPILVGADGPLSGVARAAGLGVNRRFLTGVEYELTGV
                     RGVEERLHVFLDSELAPGYIAWVVPGVHITQVGLASRRSGKPPLDAFMRRISDVFDLS
                     GARRLATRGGLIPIGGTVRRFARRDVVLVGDAAGIVSPLTAGGIHTALGSGRAAGLAI
                     VSHLMDGGPTPDVALRGTYPTFTCKRVMRRAMDLEPPNALLDLALGSPRFRDMARTIF
                     FHHRGLLSGDAWLDLLHARH"
     gene            2094..2483
                     /locus_tag="VIX62_02800"
     CDS             2094..2483
                     /locus_tag="VIX62_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5859199.1"
                     /translation="MDVKSLNQIPLFAELSRKERAQIARWADEIEEPAGYHLVDQGNF
                     AHEFFVLLDGNVEVLKDGEHLTELGRGDFFGEIALVEHERRTASVRATTPVRAIVMHE
                     RDFGAMRTQMPAVAERIEIAIKERVAR"
BASE COUNT          411 a          879 c          907 g          437 t
ORIGIN      
        1 ggacccacgg ccgtggcctg ggtgctcccg gcactcgggt tgtgctcggc cagcctgatg
       61 ctggcgacct tcgtgccgtt gcgccgagcc gcgctcctcg tgacggggtc gtggctggtg
      121 ctcgcggccg gggtgggcct catgggacct gccgcgagcc tgttccaagg gtcggcccag
      181 ctcttgttct tcgcggtcac cgtcatctgc tcactggtgc tgtcgcgacg gaggcaccat
      241 ctggagatcg cgaacctgca ctcccgccga gctttggtcg acgcggccga caacgagcgt
      301 cgacggatcg aacgcaacat ccacgacggt gcgcagcagc agttggtggc gatcggcgtg
      361 aaggccaatc tcgcacgagc catggttgcg aatgacccgg acaaggcggt cgagatcatc
      421 gaccaggtgt gcgccgatgc gaaggccgcc ctcgccgccc tccgcgacat gaccaggggc
      481 gcctatccac cgatcctggc cgacgagggg ctccccgcgg ccctcgccgc gaaggcggct
      541 gctgctccat tggccgtgac gctcgacgca ggcggggtcg gccgtctccc gaaggagctc
      601 gagatcgcga cctatttctg ctgcaccgag gcgatccaga acgctgtcaa acatgcgaag
      661 gcctcgtcga tcgaagtgac gcttcgcccg cgtgtgggtg cgttcacgtt cagcgtggtc
      721 gatgacggcg agggtttcga gccatcgacg atgcggcgag gcgtcgggat gcggagcatg
      781 gtcgagcgtg tcgaatcgct cggcggctcc ctggacgttc gatcgcgcgt tgggaaaggg
      841 acgacgatct ccgccacgat ccccttgcac ccggaccgtc gtgcacctca agcgaacgcc
      901 gacgtgacga ccgtgtgacg tgcgcgtgtc gttcgggtcg cgtgggttcc ctcgcgatgc
      961 atcgatgacc agactcggca catgccaacg agacccgacg tcgagatcgt gggtgcgagc
     1021 ttcgcgggac tcgcatgcgc gcaggcgtgc gcggcccgtg gactcgacgc ggtcgtgtgg
     1081 gaacgcaagc gcgacgcggg ggctgcggtc cggaccacgg gcatcctggt caaggaggcg
     1141 gccgatgagc tcgaccctcc ggtggaactg acccgcacgc tccacgccgt tcggctgtac
     1201 agcccctcac tcgactgggt cgacctggac gcgccgggct accgcttcct cgcgaccgac
     1261 accccggaac tcctccgtgc gctggcccgt cgcgcgcagg acgtcggagc cgacatccgg
     1321 tgggggtcgc ccttccgccc tccgcgtctc gccccgatcc tggtgggcgc cgacggtccc
     1381 ctttcgggcg tcgctcgcgc ggcgggcctc ggggtcaacc ggcggttcct caccggcgtc
     1441 gagtacgaac tcaccggcgt cagaggcgtg gaggagcgcc tccacgtctt cctggattcc
     1501 gaattggcgc cgggctacat cgcgtgggtg gtgccggggg tgcacatcac tcaggtgggg
     1561 ctcgcgagcc gacgctcggg caagcctcct ctcgatgcct tcatgcgacg gatatcggat
     1621 gtgttcgatc tctcaggtgc ccgtcggctc gctacgcgtg gcggactgat cccgatcggt
     1681 ggcaccgtgc gccggttcgc ccgccgggac gtcgtgctgg taggcgatgc tgcgggcatc
     1741 gtgtcgccgc tgaccgccgg tggcatccat accgcgctgg gctccggacg agcggcgggc
     1801 ctggcgatcg tgagccacct gatggacggg ggcccgacac cggacgtcgc actccgcggc
     1861 acctacccaa cgttcacctg caagcgcgtg atgcgccgag ccatggacct ggaaccgccg
     1921 aacgcgctcc tggacctcgc gctcgggagc ccacggttcc gtgacatggc gagaacgatc
     1981 ttcttccacc accgggggct gctgtcgggc gatgcctggc tcgacctgtt gcacgctcgc
     2041 cactaagtgc cgctggtcga gcagggaccc ggcagcggcc ccataatggg cgtatggacg
     2101 tgaagagtct gaaccagata ccgctgttcg cggaactgtc gaggaaggaa cgagcacaga
     2161 tcgcgcggtg ggccgatgag atcgaagaac cggccgggta ccacctggtg gatcagggca
     2221 acttcgccca tgagttcttc gtgctcctgg acggcaacgt cgaggtcttg aaggacggcg
     2281 agcacctcac cgaactcggt cgtggcgact tcttcggcga gatcgcgctg gtggaacacg
     2341 agcgaaggac cgcgagcgtg cgggccacga cccccgtgcg ggccatcgtg atgcatgagc
     2401 gagacttcgg agcgatgcgc acccagatgc cggccgtggc cgagcgcatc gagatcgcga
     2461 tcaaggagcg agtcgcgcgg tgaggttagg cgccgagtat ctccgccatc accctggcaa
     2521 cttggcctcg atcttcagcc aatagcgatc cgctcggagc cgaccggttc caggctacgt
     2581 ccaatgcaca aacctgcgcc gaccatcgtt cagccaggga tcagaggaac ctgc
//
LOCUS       DATDBZ010000114         3630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2905721, whole genome shotgun sequence.
ACCESSION   DATDBZ010000114 DATDBZ010000000
VERSION     DATDBZ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2905721"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VIX62_02805"
     CDS             complement(<1..417)
                     /locus_tag="VIX62_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY5859200.1"
                     /translation="MAVFEGSVLEEARAIIGRYPEGRSRSAVMPLLYLAQSEQGQVTR
                     DGLQEVAGLLDLTTAEVEAVATFYSMYRLQPTGEHLVNVCTNLACMLRGGKDVYEAAH
                     EATGIPHGGEVSEDGLFTVHEEECLGVCDFAPAVQID"
     gene            complement(417..1706)
                     /gene="nuoD"
                     /locus_tag="VIX62_02810"
     CDS             complement(417..1706)
                     /gene="nuoD"
                     /locus_tag="VIX62_02810"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019971625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEY5859201.1"
                     /translation="MQDTPRDAAKLDPAPELVSSEGEFRQETMIINMGPQHPSTHGVL
                     RLLLELDGETVVTCKPIIGYLHTGIEKNTEYRTWTQGITYVTRADYLSPFFNELGYCL
                     AVEKLLGVEAPPRAQVIRVLMNELNRISSHLVWLATGGLELGAVSVMLYGFREREVLM
                     DIFEEITGLRMNHAYIRIGGVIMDLNDEGIRKAAKFCEDMPKRIDDYETLLNNNPIWL
                     ERNVGVGVLTAEDALALGVTGPMLRSAGVAADLRKDMPYCGYETYEFDVPVRTEADAY
                     ARYEVRLAEMRESMRIVRQCLERLEEPGPIMIEDPKVGWPSKLSVGPDGIGNDPEYLR
                     HIMEESMEALIHHFKMVTEGVEVPAGEVYQAVESPRGELGFAVTSDGGHRPYRVKIRD
                     PSFVNLQATPTMVEGHLVADAIAAIASIDPVMGGVDR"
     gene            complement(1712..2212)
                     /locus_tag="VIX62_02815"
     CDS             complement(1712..2212)
                     /locus_tag="VIX62_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY5859202.1"
                     /translation="MNPAQIAEQVRERCPDTIVTRGEVTAVVARDALLETLGWLRDDP
                     DLSFGFLSSLTATDWPGKDPRFWLAYELRSMEHLHRLRVKVGLTEGDATMPTVTPMFP
                     TANWHEREVYDFFGIAFEGHPDLTRILLPDGWEGHPLLKTEELGGVNTRYHGAFIPPV
                     DTRTTT"
     gene            complement(2233..2676)
                     /locus_tag="VIX62_02820"
                     /pseudo
     CDS             complement(2233..2676)
                     /locus_tag="VIX62_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(2807..3160)
                     /gene="ndhC"
                     /locus_tag="VIX62_02825"
                     /pseudo
     CDS             complement(2807..3160)
                     /gene="ndhC"
                     /locus_tag="VIX62_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
     gene            complement(3177..>3630)
                     /locus_tag="VIX62_02830"
     CDS             complement(3177..>3630)
                     /locus_tag="VIX62_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859203.1"
                     /translation="NEDTAEGRVLSGPLPMSLNRVPQAVPGMLLIGDAAGAVNPFNGE
                     GIAYAIETAEVASELIDEALVKDRPGIAMMYPTVLRERYGDYFTIGRGFAKLIGHPAI
                     MNRATRHLLPNPRVMGFAMRLMANLTAGKDGDRQDRLFALIERIARAS"
BASE COUNT          590 a         1257 c         1175 g          608 t
ORIGIN      
        1 gtcgatctgg accgccggcg cgaagtcgca cacgccgagg cattcttcct cgtgcacggt
       61 gaacaggccg tcctcggaga cctcgccacc gtgcgggatg ccggtcgctt cgtgcgccgc
      121 ctcgtagacg tccttgccac cccgcagcat gcaggcgagg ttcgtgcaga cgttgacgag
      181 gtgttcgccc gtcggctgca accggtacat cgagtagaag gtggcgacag cctcgacttc
      241 ggcggtggtg agatccagca gcccggccac ctcttggagc ccgtcgcgcg tcacctgacc
      301 ctgctcggac tgggcgaggt agagcagcgg catgaccgcg gagcgcgacc gcccctcggg
      361 gtagcgcccg atgatggcgc gggcctcctc gagcacgctg ccctcgaaca cggccatcag
      421 cggtccaccc cgcccatcac ggggtcgatc gatgcgatcg cggcgatcgc gtcggccacc
      481 aggtggccct ccaccatggt gggggtcgcc tgtaggttca cgaacgaggg gtcgcggatc
      541 ttcacgcggt agggccggtg accgccgtcg gaggtgaccg cgaacccgag ctcgccgcgg
      601 ggggattcca cggcctggta gacctcgccg gccggcacct ccacgccttc ggtcaccatc
      661 ttgaagtggt ggatcagcgc ctccatggat tcctccatga tgtgacgcag gtactccggg
      721 tcgttgccga tcccgtcggg gcccaccgag agcttggacg gccacccgac cttcggatcc
      781 tcgatcatga tcgggcccgg ttcctcgagc cgctccaggc actggcgcac gatcctcatc
      841 gactcacgca tctccgcgag ccggacctcg tagcgcgcat acgcgtcggc ctcggtccgc
      901 accggcacgt cgaactcgta ggtctcatag ccgcagtacg gcatgtcctt gcgcaggtcc
      961 gcggcgacgc cggccgaccg cagcatcggg ccggtgacgc cgagcgccag cgcgtcctcg
     1021 gccgtcagca ccccgacgcc cacgttgcgc tcgagccaga tcgggttgtt gttgagcaac
     1081 gtctcgtagt cgtcgatccg cttgggcatg tcctcgcaga acttcgcggc tttgcggatg
     1141 ccctcatcgt tcaggtccat gatcacgccg ccgatccgga tgtacgcatg gttcatccgt
     1201 aggccggtga tctcttcgaa gatgtccatg aggacctcgc gctcgcggaa gccgtagagc
     1261 atgacgctca cggcgccgag ttcgagccca ccggtggcca gccacacgag gtgggagctg
     1321 atgcggttga gttcgttcat cagcactcgg atcacctggg cgcgtggggg cgcctccacg
     1381 ccgagcagct tctccacggc caggcagtaa cccagttcgt tgaagaacgg cgacaggtag
     1441 tcggcgcgcg tgacgtacgt gatgccctgg gtccaggtcc ggtactcggt gttcttctcg
     1501 atccccgtgt gcaggtagcc gatgatcggc ttgcaggtca ccacggtctc gccgtcgagt
     1561 tccagcagca ggcgcagcac cccgtgcgtc gaggggtgct gcggccccat gttgatgatc
     1621 atcgtctcct gccgaaactc gccctcggac gagaccaact cgggagcggg atcgagcttc
     1681 gcggcgtccc tcggcgtgtc ctgcatgtgg gtcacgtcgt ggttcgggtg tcgaccggcg
     1741 ggatgaacgc accgtggtag cgggtgttca cgccgccgag ctcttcggtc ttgagcagcg
     1801 ggtgaccctc ccacccgtcg gggagcagga tgcgggtgag gtcgggatgg ccctcgaacg
     1861 cgatgccgaa gaagtcgtag acctcgcgct cgtgccagtt ggccgtcggg aacatcggcg
     1921 tgaccgtggg catcgtggcg tcgccttcgg tcagtcccac cttcacccgg agacgatgga
     1981 ggtgttccat cgaacgcagc tcgtaggcga gccagaagcg cgggtccttg cccggccagt
     2041 cggtcgcggt gaggctggac aggaacccga acgacaggtc gggatcatcg cgcaaccacc
     2101 ccagcgtctc gagcagcgca tcgcgcgcga ccaccgcggt gacttccccg cgcgtcacga
     2161 tcgtgtcggg gcaccgctcg cgcacctgct cggcgatctg cgcggggttc acttgaaggg
     2221 ctccgtggtc cgcaccttct cctgcagctt caagatgccg tacatcaaca tctcgggctt
     2281 gggtggacac ccgggcacgt agatgtcgac cggcacgatc gtgtcgacgc cctgcacgat
     2341 ggcgtagttc gtgaacatgc caccggcgct cgcgcacacg cccatggaga tcacccagcg
     2401 cggctcgggc atctggtcgt agatccggcg cagcaccggg gccatcttct gggagacgcg
     2461 gccggccacg atcatcaggt cggcctgccg tggggatgcg cggaacagtt ccatgcccca
     2521 ccgcgacagg tcgtacttgc ccgcgccggt ggacatcatc tcgatggcgc agcaggccag
     2581 accgaacgtc gccggccaca tcgactgctt ccgcgcccac cccaccgcct tgtccaggct
     2641 cgtcaacagg acgccgcggc ggacctcgtc ctcgatctcc tcgggcgtct tgccgagctg
     2701 gacgccttcg acgcgctcaa cgggcaccga cgacctccgc ttcgtgagcc tgatcctcgt
     2761 cctcggcgat cgcggccgcc tccacgggcg tgaccgtgtc ctcggtccag tccagcccac
     2821 ccttgcgcca gacgtagacg taggcgacga acaccaggcc gatgaacacc gccatctcga
     2881 tcaacccgaa caacccgagc cgacggaacg agaccgccca cgggaacagg aagatcgtct
     2941 cgacgtcgaa gatgatgaag agcatcgcca ccacgtagaa cttcacgctg aacttctcac
     3001 cacgaggcag gcgcaccggg tcgttgccgc attcgtaggc cgacagcttc accgggttcg
     3061 ggcggttggg ccggaaccag cgcgacacga tcatcgacac gaccccgaag gccaccgcca
     3121 gcgccgccat cacgacgatc gggaggtagt cgatcagcac ggcgagcatc ctagcgtcat
     3181 gacgcgcgag cgatccgttc gatgagcgcg aatagacggt cctgccggtc cccgtccttg
     3241 ccagcggtga ggttggccat gagccgcatc gcgaacccca tcacccgcgg gttcggcaac
     3301 aggtggcgcg tggcccgatt catgatcgcg ggatggccga tgagcttggc gaacccacga
     3361 ccgatggtga agtagtcgcc gtagcgctcg cgcagcacgg tggggtacat catcgcgatg
     3421 cccgggcggt ccttcaccaa cgcctcgtcg atcagctccg acgcgacctc ggccgtctcg
     3481 atcgcgtacg cgatgccttc cccgttgaag gggttcaccg cgccggccgc gtcgccgatc
     3541 aggagcatgc ccggcacggc ctgcggcacc cggttgaggc tcatcggcaa cggaccggac
     3601 agcacccttc cctcggccgt gtcctcgttg
//
LOCUS       DATDBZ010000115         3544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2292696, whole genome shotgun sequence.
ACCESSION   DATDBZ010000115 DATDBZ010000000
VERSION     DATDBZ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292696"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VIX62_02835"
     CDS             <1..224
                     /locus_tag="VIX62_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859204.1"
                     /translation="PLLPTILVGVGTAAGVMFYQVVAFLLGQLEETFAYAVRVTLLTA
                     LYGAILTPIVYPLLRRVIEGSRPTRVVRF"
     gene            225..2183
                     /locus_tag="VIX62_02840"
     CDS             225..2183
                     /locus_tag="VIX62_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5859205.1"
                     /translation="MEKSSARLKVFAILVLVMFAALSTRLWFLQVLATEVYAEEAQNN
                     GVRTVAIDALRGEIWTADQYGKPNGVPLVKNRSSLEVRVDVQELEASGIAEQVLGELS
                     TMLDVPVKQIRRDLASTQYFDYQPKPIAEFVPEEVAFSIRERQQDFPGVIVRNASVRE
                     YPMGRTASHIVGSVGLVQPAQLEAAKPGEYGPNDAAGQAGLELEYERWLRGRKGLQRY
                     VVNSDGERIRELGGREPSAGADLVLTMDGEWQRAAEDALLDTIYRTRQVYDEDSGTNY
                     KADAGVVVVLDVETGGVKAMASWPDYDPRWYVKGLTNGQLCYLGYDPKCQGANEVAPL
                     LNRAYQEVYLPGSTFKPFTALAAVKEGYANFGTYYPCPSEYIHGTDESGTTFNNWSNV
                     DLAARTLDEHLIISCDTSFYAWGSDFWYRYQRDPLGENNEPLQKDLRAWGFEKPTGID
                     LPGEASGFLPDAEWGNDPDQKYLFGKGGWNPGGYILTMIGSGYMSVTPLQLATAYGAL
                     ANEGHLCRPHVVDRVVASDGEVIKDVKGQCDRTVPYTKAELDYVRNALTQVPVRGTAT
                     TPFRGFPLSEIPVAGKTGTAYRGTLFQDTSWFAAMVPANDPKYVVVAMVEQAGFGSDV
                     AAPLVRRMIETMYGIESDAPVITPSGQD"
     gene            2183..3334
                     /gene="rodA"
                     /locus_tag="VIX62_02845"
     CDS             2183..3334
                     /gene="rodA"
                     /locus_tag="VIX62_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY5859206.1"
                     /translation="MDVVAGRVWVDERRPIRHLDPTLLMVTGALVVIGMFAIYSATSQ
                     TLRADGFDPFSRVNKQAVTAVLGFVLLLVMATFDYRFLKVYAGFIYGATLVGLLLLRI
                     PGVAASETGTSNYINIPGVSLLQISPSEFAKIGLIVILAAMLSELRTTVPQVPDLVRV
                     IGVAVLTMMLVFLNVEIGTTIVLAAITVGVLVVAGTQAKHLIALAAAAVVLIVLAFQL
                     NVVEEYQVERLLTVFDSGSVSEDARYNLEQSVIAVGSGGMFGRGFLQGSQTNLDYVPE
                     QHTDFIFTVVGEEFGFVGSAFVLALFALLLWRAIRIAYLSKDAFGTFLATGVASMFAI
                     QIFVNVGMVIGIMPITGIPLPFLSYGGTAMLVNFVAIGILLNVHMRRFK"
     gene            complement(3327..>3544)
                     /locus_tag="VIX62_02850"
     CDS             complement(3327..>3544)
                     /locus_tag="VIX62_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5859207.1"
                     /translation="DRGAGVDPQALHRIFEPLEQAEDLNTRTHQGVGLGLTLARMSAN
                     AMDGDVVLESTGPEGSTFLWTVTRPVT"
BASE COUNT          557 a         1174 c         1228 g          585 t
ORIGIN      
        1 cgccgctgct gcccacgatc ctcgtggggg tcggcacggc ggccggcgtg atgttctatc
       61 aggtcgtggc cttcctgctc ggccagctcg aggagacgtt cgcctacgcc gtgcgcgtga
      121 cgctgctcac ggccctgtac ggggcgatcc tcaccccgat cgtctacccc ctgctgcgtc
      181 gggtgatcga gggctcgcgc ccgacgaggg tggtgcgctt ctagatggag aagagctcgg
      241 cccgcctcaa ggtgttcgcg atcctcgtgc tcgtgatgtt cgccgcgctg tcgacccggc
      301 tgtggttcct acaggtgctc gccacggagg tctacgccga ggaggcgcag aacaacggcg
      361 tgcgcaccgt cgcgatcgat gccctgcggg gcgagatctg gacggccgac cagtacggga
      421 agcccaatgg cgtgccactc gtgaagaacc gcagcagcct cgaggtgcgc gtcgacgtgc
      481 aggagctgga agccagcggc atcgccgaac aggtgctcgg cgagctctcc acgatgctcg
      541 acgtgcccgt gaagcagatc cggcgtgatc tcgccagcac gcagtacttc gactaccagc
      601 cgaagccgat cgccgagttc gtgcccgagg aggtcgcgtt ctcgatccgc gaacgtcagc
      661 aggacttccc cggggtgatc gtgcgcaacg cgagcgtgcg cgagtacccg atggggcgca
      721 ccgcgtcgca catcgtgggc tcggtcggcc tcgtgcagcc cgcgcagctc gaggccgcca
      781 agcccggcga gtacgggccg aacgacgccg cgggccaggc gggtctggag ctcgagtacg
      841 agcggtggct gcgagggcgc aaggggctgc agcgatacgt cgtgaactcc gacggggagc
      901 gcatccgcga gttgggcggc cgcgagccga gcgccggcgc cgacctcgtg ctcacgatgg
      961 acggcgagtg gcagcgcgcc gccgaggacg ccctcctcga cacgatctac cgcacccggc
     1021 aggtctacga cgaggacagc ggcacgaact acaaggccga tgccggcgtg gtggtcgtgc
     1081 tcgacgtcga gaccggcggc gtgaaggcga tggcttcttg gcccgactac gacccccgct
     1141 ggtacgtcaa ggggctcacg aatggccagc tctgctacct gggctacgac ccgaagtgcc
     1201 agggtgcgaa cgaggtggcc ccgctgttga accgcgccta ccaggaggtc tacctgccgg
     1261 gctcgacctt caagccgttc acggcgctcg cggcggtgaa ggagggctac gcgaacttcg
     1321 gcacctacta cccgtgtccc tccgagtaca tccacggcac ggacgagtcg ggcaccacgt
     1381 tcaacaactg gtccaacgtc gacctcgccg cgaggacgct cgacgagcac ctcatcatct
     1441 cgtgcgacac gagcttctac gcctgggggt cggacttctg gtaccgctac cagcgcgacc
     1501 cgctcggcga gaacaacgag ccgttgcaga aggacctgcg cgcgtggggg ttcgagaagc
     1561 cgaccggcat cgacctgccg ggcgaggcga gcggtttcct gcccgacgcg gagtggggca
     1621 acgaccccga ccagaagtac ctgttcggca agggcggctg gaaccccggt ggctacatcc
     1681 tcacgatgat cggctcgggg tacatgtcgg tgacgccgct gcagctcgcg accgcctacg
     1741 gagcgctcgc caacgagggg cacctgtgcc gcccgcacgt ggtcgatcgc gtggtcgcga
     1801 gcgacggcga ggtcatcaag gacgtcaagg ggcagtgcga ccgcacggtg ccgtacacga
     1861 aggccgagct cgactatgtg cggaacgccc tcacgcaggt gccggtgcgc ggcacggcga
     1921 cgactccctt ccgcgggttc ccgttgtcgg agatcccggt ggccggcaag accggcaccg
     1981 cgtaccgagg cacgctgttc caagacacgt cgtggttcgc ggcgatggtg ccggccaacg
     2041 atccgaagta cgtggtcgtg gccatggtgg agcaggccgg gttcggctcc gacgtggccg
     2101 cgccactcgt gcggcgcatg atcgagacga tgtacggcat cgagtcggac gcaccggtca
     2161 tcacgccctc ggggcaggac tgatggacgt cgtcgcgggg cgcgtatggg tcgacgaacg
     2221 ccggccgatc cgacacctcg accccacgct gctcatggtg acgggcgccc tcgtcgtgat
     2281 cggcatgttc gcgatctact cggccacgag ccagaccctg cgcgccgacg ggttcgatcc
     2341 gttctcgcgc gtgaacaaac aggcggtcac ggcggtgctg ggcttcgtgc tactgctcgt
     2401 gatggcgacg ttcgactacc gcttcctgaa ggtctacgcg gggttcatct acggggccac
     2461 gctggtgggg ctgctgctcc ttcgcatccc gggcgtcgcc gcgagcgaga ccggcacgag
     2521 caactacatc aacatccccg gcgtgagcct gctgcagatc agcccgtcgg agttcgccaa
     2581 gatcgggctc atcgtgatcc tcgcggcgat gctgtcggag ttgcgcacca ccgtgcccca
     2641 ggtgcccgac ctcgtgcgcg tgatcggtgt ggcggtgctc acgatgatgc tcgtcttcct
     2701 caacgtggag atcggcacga cgatcgtgct ggccgcgatc acggtggggg tgctggtcgt
     2761 ggccggcacc caggccaagc acctcatcgc cctcgcagcc gcggcggtcg tgttgatcgt
     2821 gctcgccttc cagctcaacg tggtcgagga gtaccaggtc gagcgcctgc tcacggtgtt
     2881 cgactcgggc tcggtgagcg aggatgcccg ctacaacctt gagcagtcag tgatcgcggt
     2941 cggctcgggc ggcatgttcg ggcgtgggtt cctgcagggg tcgcagacca acctcgacta
     3001 cgtgcccgag cagcacaccg acttcatctt cacggtggtg ggggaggagt tcgggttcgt
     3061 cggctcggcg ttcgtgctcg cgctgttcgc gctgctgctg tggcgcgcga tccgcatcgc
     3121 ctacctgtcg aaggacgcgt tcggcacgtt cctcgcgacc ggggtggcgt cgatgttcgc
     3181 gatccagatc ttcgtgaacg tgggcatggt catcggcatc atgccgatca ccggcatccc
     3241 gctgccgttc ctgagctacg ggggcacggc catgctcgtg aacttcgtgg cgatcggcat
     3301 cctgctgaac gtgcacatgc gccggttcaa gtgaccggac gcgtcaccgt ccacaggaac
     3361 gtcgaaccct caggcccggt cgactccagc acgacgtcgc cgtccatcgc gttggccgac
     3421 atccgcgcga gcgtgaggcc gagcccgaca ccctgatggg tgcgggtgtt gaggtcctcg
     3481 gcctgctcca ggggttcgaa gatccggtgc agcgcctgcg gatcgacgcc ggcaccgcga
     3541 tcga
//
LOCUS       DATDBZ010000116         5798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_246162, whole genome shotgun sequence.
ACCESSION   DATDBZ010000116 DATDBZ010000000
VERSION     DATDBZ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246162"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VIX62_02855"
     CDS             complement(<1..283)
                     /locus_tag="VIX62_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859208.1"
                     /translation="MPSPLRSALSDLSWKRLVVIAIGAVVLFVVVVLVARGLRTLPGV
                     QEFIVEYPGHTDLPEGAPVGIPAWLAWQHFFNAFLLVMIVRTGLIVRSKV"
     gene            complement(512..1744)
                     /locus_tag="VIX62_02860"
     CDS             complement(512..1744)
                     /locus_tag="VIX62_02860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5859209.1"
                     /translation="MSAEEASGSPDAANRAIRTDSVTGPHPSRYPGAAAGRPRLATWQ
                     EAPDNRWAFAHLGELLPTATVARTRPAEPADATVRLGALTPALPDLEARLEQSYTDAF
                     LVLHGEEVLAEYYRPGFAPDGLHLVMSVSKSLCGIVVGALIDESRIDHERPVTDYVPE
                     LAGSVYDGPSVRHVLDMQIAIDYDEDYTNPASEVQAHDRAAGWRDRYTNDLEDTYSFL
                     TTLRGSGSVGEFQYCSANTDVLAWVVERVTGLRYSDALSVHLWSKLDADHDATITVDT
                     AGFGFANGGVSCTARDLARVGRVMLDGGLAPGGRVVSEEWASAVLDGGDREAMTYEGF
                     TTAFPNGSYTNQWWCMGNERGNVSGIGIHGQHLWLDPQTDSVIVKLSTWPDPDSAFWH
                     GVQSELLLDVSQALDSFA"
     gene            complement(1741..3177)
                     /locus_tag="VIX62_02865"
     CDS             complement(1741..3177)
                     /locus_tag="VIX62_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY5859210.1"
                     /translation="MPTSTDPATSPAFVDTASRPETRGIELVSPSDRHGRARDLFWVW
                     AAPNVSVLNFTIGATMILLGLELWQAILVIVAGSLPWVFPGILAIAGPAAGTSGSVIT
                     RALYGIMGNKLVVAFTGWLISAVFLALNWLASSFMGADLLAQWGLTDPVLGPVLVTVV
                     VSTITVLVAVYGHALIIKSYSVITIVLLAVFLAVTAFILPRVDWSYRAAEPLDGVPLW
                     SAMTIGFTILASTPLSYVNSPDIARYLPRSTRPSHIIAATAFGGALPCIFFTIVGALL
                     ATAVSDTAMADGIETALLDLVPGWLGPLFVLGVIVNTVALNGMTTYTSSMALQAVGVP
                     IRRIPSAIVVGVIGTALTLYLVMSTNLLDAVNLMLQFLVIVSAPAMAIFTTDIILRRN
                     RYDGAALFDEDRGGPFWYTGGWSIPGMGAVVTGGLATALCLSTDIWTGPISEAIGYID
                     LSVPAGMIVAAGVYLGLSKTSLGRSTLR"
     gene            3291..3425
                     /locus_tag="VIX62_02870"
                     /pseudo
     CDS             3291..3425
                     /locus_tag="VIX62_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            3564..3926
                     /locus_tag="VIX62_02875"
     CDS             3564..3926
                     /locus_tag="VIX62_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859211.1"
                     /translation="MVEHVESAEGRDIARLWLNARHLCRFTPALAAALVEQEALDRAR
                     LLELIEAGVSTGEFTADPFDACLRIFIAVDGYGAYVNDVGAFEHDAYTRFVTDATEWA
                     LGLDPGQLRAAIAELQPS"
     gene            complement(4389..4460)
                     /locus_tag="VIX62_02880"
     tRNA            complement(4389..4460)
                     /locus_tag="VIX62_02880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4425..4427),aa:Gln,seq:ctg)
     gene            complement(4594..>5798)
                     /locus_tag="VIX62_02885"
     CDS             complement(4594..>5798)
                     /locus_tag="VIX62_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEY5859212.1"
                     /translation="GIIERLKAAGHLYESYSTAEEIDARNAAAGRPKQLGYDNFDRDL
                     TDAQRAAFRAEGRQPALRLRVPEADLGFDDLVRGRIDFPIGSTIDFVVVRPNGAPLYT
                     FVNPVDDALMGITDVLRGEDLLSSTPRQIALYHALIDIGVTTFVPRFGHLPYVMGEGN
                     KKLSKRDPESNLFHHRDRGFIPEGLVNYLALLGWGFSADRDVFSRDELVAAFDVVNVN
                     PNPARFDLKKAEAINGDHLRQLEVGDFAARTVPYLQAAEAVSTPITPGEEAILVEAAP
                     LVQERIGLLGEAPGMLGFLFTDSAGLEYDEAAVGALPADTGAVLAAAREALDRLPADS
                     WTHTEIEEALRAALIDGLGLKPRVAFGPVRTAVSGRRVSPPLFESMQILGKVDSLARI
                     DRLAELIG"
BASE COUNT         1011 a         1847 c         2041 g          899 t
ORIGIN      
        1 gcaccttcga ccgcacgatg agtccggtgc gcacgatcat gaccagcagg aaggcgttga
       61 agaagtgctg ccacgcgagc caggcgggga ttccgaccgg cgcaccctcg ggcaggtcgg
      121 tgtggccggg gtactccacg atgaactcct gcacgcccgg tagggtgcgc agtccacgcg
      181 cgacgagcac gacgacgacg aacagcacga cggcaccgat cgcgatcacg acgagccgtt
      241 tccatgagag atccgacagc gcggaacgta agggtgacgg cacgcccatc agcgtgacaa
      301 accggggcgt gggatgcaac tccgcgcggt cgagcgctgc ggactgccgt gacgcccggg
      361 gcaagaagaa agcccggcga accgggcacg cggctcgcat cgacgggccc gatcggagca
      421 gtgcgttcgg cgtcgttcga atcaggaagt tgcatgctga gggtccttgg atgtcgttga
      481 gaagtggccg ccccgtggcg tgccctgtgt cctaggcgaa tgagtcgagc gcctggctca
      541 cgtcgagcag caactcgctc tgcaccccgt gccagaacgc cgagtcgggg tcgggccagg
      601 tcgagagctt cacgatgacg gaatccgtct gcggatcgag ccagaggtgc tggccgtgga
      661 tgccgattcc gctcacgttg ccgcgttcgt tgcccatgca ccaccactgg ttcgtgtacg
      721 aaccgttggg gaacgccgtg gtgaagccct cgtacgtcat cgcctcgcgg tcaccgccgt
      781 cgaggaccgc gcttgcccac tcctccgaca cgacccggcc tccgggggcg agcccgccat
      841 cgagcatgac ccggccgacc cgtgcaaggt cgcgcgccgt gcacgagacc ccgccgttcg
      901 cgaagccgaa gccggcggtg tccacggtga tggtggcgtc gtgatccgcg tcgagtttcg
      961 accagaggtg gaccgacagt gcgtcgctgt agcgcagacc tgtgacccgc tcgacgaccc
     1021 aagcgagtac gtcggtgttc gccgagcagt actggaactc gcccaccgaa ccgctgccac
     1081 gtagcgtcgt gaggaacgag tatgtgtcct ctaagtcatt ggtgtatcgg tcacgccagc
     1141 ccgctgcgcg gtcgtgcgcc tgcacctcgg aggcggggtt cgtgtagtcc tcgtcgtagt
     1201 cgatggcgat ctgcatgtcg agcacgtgcc ggacgctcgg cccgtcgtag accgagccgg
     1261 cgagctcggg cacgtagtcg gtcaccggcc gctcgtggtc gatgcggctc tcgtcgatga
     1321 gtgcgccgac gacgatcccg cagagcgact tcgagacact catcacgagg tggaggccgt
     1381 ccggagcaaa gcccggtcgg tagtactccg cgagcacctc ttcgccgtgt aacacgagga
     1441 acgcatcggt gtaactctgc tcgagccgcg cctccagatc gggcagcgcg ggggtcagcg
     1501 cgccgagccg caccgtggcg tcggccggct cggccggcct cgtgcgcgcg accgtcgcgg
     1561 tcggcagcag ctcaccgagg tgcgcgaatg cccagcgatt gtccggcgct tcctgccagg
     1621 tggcgagacg tggccgaccg gccgccgcac cgggatagcg gctggggtgg gggccggtca
     1681 cgctgtccgt tcggatcgca cggttcgcgg catccgggct gcccgaggcc tcctcggcgc
     1741 tcatcgaagc gtgctccggc cgagcgaggt cttcgagaga ccgaggtaga cgcccgcggc
     1801 gacgatcatg ccggccggca ccgagaggtc gatgtagccg atcgcctcgg agatcggacc
     1861 cgtccagatg tcggtcgaca ggcagagcgc ggtggcgagt cccccggtca cgaccgcacc
     1921 catgcccggg atgctccagc ccccggtgta ccagaagggc ccgccgcgat cctcgtcgaa
     1981 gagcgcggcg ccgtcgtagc ggttgcggcg gaggatgatg tcggtcgtga agatcgccat
     2041 cgcgggggcg gagacgatca cgaggaactg cagcatgagg ttcactgcgt cgagcaggtt
     2101 cgttgacatc accaggtaga gggtgagggc cgtgccgatg acgcccacga cgatggcgga
     2161 ggggatgcgg cggatcggta cgccgaccgc ctggagcgcc atgctggacg tataggtggt
     2221 catgccgttc aaggcgacgg tgttcacgat gaccccgagg acgaagagcg gaccgagcca
     2281 gcccggaacc aggtcgagca gggccgtctc gatgccgtcg gccatcgccg tatcgctgac
     2341 ggcggtggcg agcagtgcgc cgacgatcgt gaagaagatg cagggcaggg cgccgccgaa
     2401 ggcggtcgcg gcgatgatgt gcgagggcct ggtcgagcgc gggaggtagc gggcgatgtc
     2461 ggggctgttg acataggaga gcggtgtcga ggcgaggatc gtgaagccga tcgtcatcgc
     2521 cgaccacagc ggcacaccgt cgagcggctc cgccgcccgg tagctccagt cgacgcgggg
     2581 caggatgaac gcggtcaccg cgaggaagac cgcgaggagc acgatggtga tcaccgagta
     2641 gctcttgatg atgagcgcgt gcccgtagac cgcgacgagc accgtgatcg tcgacacgac
     2701 gaccgtcacg agtacgggcc ccagtacagg gtcggtgagg ccccactgag cgagcagatc
     2761 ggcacccatg aatgaggacg ccagccagtt gagggcgagg aagaccgccg agatgagcca
     2821 ccccgtgaac gcgacgacga gtttgttgcc catgatgccg tacagtgcgc gagtgatcac
     2881 cgaaccggag gttccggcgg ccggaccggc gatggcgagg atgccgggga acacccacgg
     2941 cagcgaaccg gcgacaatga cgaggatcgc ctgccagagc tcgagaccga gcaggatcat
     3001 tgtcgcgccg atggtgaagt tcagcacgct gacgttgggt gcggcccaca cccagaacag
     3061 gtcgcgggcg cgtccgtgcc gatcggacgg gctcaccagc tcgatgcctc gcgtctcggg
     3121 gcggcttgcg gtgtcgacga aggccggcga cgttgccggg tcggtgctgg ttggcacgag
     3181 gatcctctcg gagttctgga gccggtgccg tcgacctgcc gaggcggcac cactattggt
     3241 cgaatgacca attggctatt ggtcgctcat ccaataacat gaatcgcatg atgtcaagtg
     3301 ctccggtcgt gcgctcgcga aagcgaccca gcgagcgccg ggccgagatc gtgcgtgaag
     3361 ccgccggtgt ggcgcttgcc gatgggctcg agcgcatcac cctccgcgcc gtcgccgagc
     3421 gactcggcgt tcggccgggc ctgatcaccc actacttccc aatcgccgaa gacctcgtca
     3481 tcgccgcgtt cgtgcaggcc gtcgcagacg agcgcgacgc aatggtgccc gagcacggcg
     3541 cgccgctcga acgactcgcg cgaatggtcg agcacgtcga gagcgccgag ggtcgcgaca
     3601 tcgcgaggct ctggctgaat gcgcgtcacc tctgccggtt cactcccgct ctcgccgccg
     3661 cgctcgtcga acaggaggcg ctcgaccgcg cgcgattgct cgaactcatc gaggccggcg
     3721 tctcgacggg cgaattcacc gcagatccgt tcgacgcgtg ccttcgaata ttcatcgccg
     3781 tcgacggcta cggagcctac gtgaacgacg tcggcgcctt cgaacatgac gcatacaccc
     3841 gctttgtcac cgacgccacc gagtgggcgc tcggcctcga tcccgggcaa ctcagggctg
     3901 cgatcgccga actacaacca agctgacgca tctcgcagag caccgttcga tgccgatcgg
     3961 tcaacgggcg attcgggcga tctcgatttc cggtcagcca tccgtttgta ggaagctgcc
     4021 atcttccgtg gaacttgtag gaagctgcca tcttccgtgg aattgagccc tcgaaccggt
     4081 gacaaatacc gtggaatcgt ggcctctgat gccatgttca ctgggaaccc acatgcgcca
     4141 tcacagaaga gccgggcacg gagtgcgact tgccacccac cacacgcacc agtggagagc
     4201 tcgacaggat gcgaatccgc gtctagttca cccacatgtt cacgacccca gcacctcgtc
     4261 gaggatctct gcggtaatcg ccgctgagcg ggtttctggt gtcgtttctg gtaactgcgc
     4321 ccgaaaaact gtgaaggcgc aggaggggca atgggtgata ttgccgcgga atcctgcgcc
     4381 tgtcagtgct ggggtacctg gactcgaacc aagaacaacg gtaccagaaa ccgccgtgtt
     4441 gccaattaca ccatacccca atgacctgaa accgaagccc cgggccgaga gtcaactgta
     4501 gcctaccgcg ccgccccggc caaactgagc gcagctcgct ggccggggaa cgatggccgc
     4561 gatccgcgcg tcgcgaacct cgactgtgcg cgctcagccg atgagctcgg cgagccggtc
     4621 gatgcgggcg agcgagtcga ccttgccgag gatctgcatc gactcgaaca gcggtggcga
     4681 gacacgtcgg cccgagacgg cggtgcgcac agggccgaac gcgacgcgcg gcttgagccc
     4741 gaggccgtcg atgagggccg cgcgcagggc ctcttcgatc tcggtgtgag tccacgagtc
     4801 ggccggcagc cggtcgagcg cctcgcgagc cgcggcgagg accgcaccgg tgtctgccgg
     4861 gagcgcgccg accgcggcct cgtcgtattc gagcccagcg gagtcggtga agaggaagcc
     4921 gagcatgccg ggagcctcgc cgagcaggcc gatgcgctcc tgcacgaggg gcgcggcctc
     4981 gacgaggatc gcctcttcgc cgggagtgat cggcgtcgag accgcctcgg ccgcctgcag
     5041 gtagggaacg gtgcgcgcgg cgaagtcgcc gacttcgagt tgacgcaggt ggtcgccgtt
     5101 gatggcctcg gccttcttca ggtcgaagcg ggccgggttc gggttgacgt tcacgacgtc
     5161 gaacgcggcc accagctcgt cgcggctgaa cacgtcgcgg tcggccgaga agccccagcc
     5221 gagcagtgcg aggtagttga cgaggccttc ggggatgaac ccgcggtcgc ggtgatggaa
     5281 caggttcgat tcggggtcgc gcttcgagag cttcttgttg ccctcgccca tgacgtaggg
     5341 aaggtggccg aagcgcggca cgaaggtcgt cacgccgatg tcgatgagcg cgtggtacaa
     5401 cgcgatctga cggggggtcg acgagagcag gtcttcgccg cgcagcacgt cggtgatgcc
     5461 catgagcgcg tcgtcgacgg ggttcacgaa cgtgtagagc ggtgcgccgt tcgggcgcac
     5521 gaccacgaag tcgatcgtcg atccgatcgg gaagtcgatg cgaccgcgca cgaggtcatc
     5581 gaagccgagg tcggcctcgg gcacgcgcag tcgcagggcc ggctggcgac cctcggcccg
     5641 gaacgccgcc cgctgcgcgt cggtgaggtc gcggtcgaag ttgtcgtagc cgagctgctt
     5701 gggccggccg gcggctgcgt tgcgggcgtc gatctcttcg gcggtcgagt agctctcgta
     5761 gaggtgcccg gcggccttca gccgttcgat gatgccgg
//
LOCUS       DATDBZ010000117         9841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1677730, whole genome shotgun sequence.
ACCESSION   DATDBZ010000117 DATDBZ010000000
VERSION     DATDBZ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677730"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VIX62_02890"
     CDS             <1..212
                     /locus_tag="VIX62_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5859213.1"
                     /translation="LDSSTSHDILELLRRSVEEYGQTIVMVTHDASAAGYADRVVFLA
                     DGLIVDEMREPTAASVLDRLKSLEG"
     gene            218..2764
                     /locus_tag="VIX62_02895"
     CDS             218..2764
                     /locus_tag="VIX62_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5859214.1"
                     /translation="MLRATLKGLLARKRRLALTVISIVLGVGFVAGTFVLTDTMNKAF
                     DQLFTDAAAGSDVVVRAEQAFAPSATGPGSGAIEERDPVPDTLLPTVESVPGVASASG
                     DVSGSAQVVDPATGDPIGGVGPPTIGTNWTDTNPTVQIREGAAPSAADDVVIDAATAT
                     RYNLAVGQDVKILFQGPPEEFTISGILGFGDADNLGGATLAAFTLPTAQRVLDKEGEF
                     DSISVLGDEGTDPGQLRAAIQDVLPPKVEAVTSTSVADEQSDQLKEGLGFFRIALLVF
                     AFIALFVGSFQIFNTFSIIVAQRAKELALLRAVGASRRQVLTSVIIEALVLGLVAAII
                     GIVAGVGIAVLLKALLSGFGIDLPSTSLQLEPRTIVVSLIIGVVVTTVASVLPARRAA
                     RVAPIEALRDSQDAGADHLGRRSVIGVIVTALGLAFLGYGLFGEPDNAGLLIGGGAAV
                     IFIGIAILSPLAARPLSGAIGRPVRGLSMAARLGRENAMRNPRRTAATSSALMIGLGL
                     IAMVSILSASLKASFDAALQDTLRADLVLTSSSFLPFSQEVAARTADVDGVEAVSAFR
                     QGGFKVNDSDAAMTGVDPATIETVANLGPSEGAIASLDDGGVLVYDQTMEDNGWAVGD
                     ELPSAFATIGDDPLTIGGTFDDNRLVGDYVVSLDTFDELFRAQLDTFVFVKVADGADI
                     AAVRGDVEQATAEFGNIEVQDQAAFRDQQAGFVNQLLGLVTAMLFLAVVIALFGIANT
                     LSLSIFERTRELGLLRAVGMGRTQVKRMIRWESVIIAILGALFGIAIGIFFGWALQQA
                     LAPEGITEFVLPVGQLVFFLILAALAGVVVALLPARRAAKLNVLEAISYE"
     gene            2785..4737
                     /gene="thrS"
                     /locus_tag="VIX62_02900"
     CDS             2785..4737
                     /gene="thrS"
                     /locus_tag="VIX62_02900"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY5859215.1"
                     /translation="MAAMPTLTLPDGAAIELPEGEPIGSALPKGTIAARVDGELRDLS
                     FVPAADAAVEAIAPGSEDGLHVLRHSTAHVLAQAVCDLHPGTKYAIGPAVTDGFYYDF
                     DLPETIGTAELAKIDRRMRQIVKQNQPFVREEVSRAEAAERLADQPFKLEIIAGLDND
                     DDPEASEGETVSLYRNGEWADLCMGPHVPHTGKLGAFKLTNVAGAYWRGDEKNPQLTR
                     IYGTAWATKDDLEAHLHRLEEAERRDHRKLGLELDLFSFPDEIGSGLAVFHPKGGLIR
                     RLMEDYSRRRHEEAGYEFVNTPHITKRELFETSGHLEWFADGMFPPMELDGGTEYYLK
                     PMNCPMHILIYRSRTRSYRELPLRLFEFGTVYRYEKSGVIHGLTRVRGLTMDDAHIFT
                     TKEQMGEELRSLLVFVLDLLRDYGLDDFYLELSTRPEDKAVGSDEDWAEATEALRLAA
                     ESMDLELVMDEGGGAFYGPKISVQARDAIGRTWQMSTIQVDFQFPQRFDLHYAGPDGE
                     RHRPIMIHRALFGSVERFFGVLTEHYAGAFPLWLAPEQVRFVAVADRHVEHSEELAAK
                     AKDAGLRAFVDDSKESVGKKIRAAQLMKAPYTLVIGDQELESGLYTVRDRAGTEHQGV
                     EFDDLVTALLAEADSRALEQTDFGAA"
     gene            4738..5223
                     /locus_tag="VIX62_02905"
     CDS             4738..5223
                     /locus_tag="VIX62_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY5859216.1"
                     /translation="MDHLWSPWRMEYIRSGSEERGVTDGACVFCVIPDREPERVLATG
                     ATAYIVLNKFPYNPGHLLVVPLRHVGDLEELSSDELLEVQMLIQRSVRALREDQPHGF
                     NVGLNLGAGAGAGIPEHVHWHVVPRWNGDTNFMSVVGETRVLPELLADTARRLAPRFA
                     E"
     gene            5220..6035
                     /locus_tag="VIX62_02910"
     CDS             5220..6035
                     /locus_tag="VIX62_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5859217.1"
                     /translation="MSTVMDGTVALPDGGHLAYDLAGEGPMLTMIHPGLWDRRTWDRE
                     FDAWTDRYRVLRYDVRGYGRSSRLTGASYSHARDLAVLLENMGIEQTSIVGCSMGGAI
                     AIDFVLVEPTKVVSLVLAAAGLGGFEANEEENAWWEARMDPVEAAVEAGDLERAEDLR
                     LEVWAPMGTDDAAGRRIREIAFDNIHELTMDESAEEELEPPAALRLGEIDVPTLVLVA
                     EHDPPFMRRCDELIGREVLDGRLVRIDADHVVNVRNPEAFDAAVLPFLAEYAL"
     gene            complement(6051..8141)
                     /gene="fusA"
                     /locus_tag="VIX62_02915"
     CDS             complement(6051..8141)
                     /gene="fusA"
                     /locus_tag="VIX62_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY5859218.1"
                     /translation="MKTYDAKDIRNVLLVGHGGSGKTTLLEAMLFTSGAITRMGTVED
                     GNTVSDHDPDEQRKGISVSLAMAPVEWNGVKINVLDAPGYADFIGDVRSAIRAVDAVI
                     VVVSAVDGVEVQTEAAWELAVEEGLPRAIFVNKLDRERADFQATLDQLVSSFGNQVAP
                     FELPIGQEHEFKGVADLLHEKADLYPSGPKAEESDWPDDLHAMADPAREKLIEAVAES
                     DDALIEEYLEVGTLPEEHIVSGAKDGFARARLAPVIVGSATKAMGIDRLLDFIVEEFP
                     SPLERGPITVIGKDGTESERPSEENGPTTAFVFKTVSDPFVGHITMFRVFSGAIQPDT
                     SVHNSTQGTDERIGQLFTQRGKEHESVPAVSAGDIGAVAKLQHAHTGDTFATKDAPVH
                     LRPLELPEPLLAYAIAPKAKGEEDKLATGLARLREEDPTLRVSRSEETHETVMHGMGE
                     AHLEVMIERLKGKFGVDVVTSPAKIAFRETIRTKATATGRHVKQSGGHGQYAVCVIDV
                     EPLPHGEGFVYESKIFGGAIPQQFIPSIEKGIVKTMVDGVVSGNPMVDIKVTLTDGKF
                     HTVDSSDMAFQIAGSMALKEAVDQAGVVLLEPVVDLEVVVPEAYTGDVMGDLNAKRGK
                     IAGMEQVGGGKQRIKAQVPQAEVARYVIDLRSMTGGRGAFSMTFSHYEQLPQHLAQKV
                     IDDYQKSREEAHKK"
     gene            8207..8737
                     /locus_tag="VIX62_02920"
     CDS             8207..8737
                     /locus_tag="VIX62_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859219.1"
                     /translation="MLRFERQPPGTALTRSTLVAGALVVVAAGWFVVAREPEVAAAFA
                     AAASVLLLVGGHRANHGAGGPTDRMLDELLDRAWDGVILGTIAWVARIATPTTAVAAL
                     VALCASFLSAYVRARGASLSYSVEESHITRGMRYALVCAGLAFGWLTWTLWVAAAMSV
                     LAVVVRTSQVAREERA"
     gene            8734..9627
                     /locus_tag="VIX62_02925"
     CDS             8734..9627
                     /locus_tag="VIX62_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEY5859220.1"
                     /translation="MRGSEETPKQTVIFHVYRAVAWLGRTLPERFGRRLFRFGGLLAH
                     DLMPGVRRTVAFNQAQVLGRPPGDPLVRASTREAFDLYARYWFDSFHVVGETDETMIE
                     RFTTVDAHHMFDPLEAGTGVIIAMPHVGDWDAAGRWLLAEGHRCVSVAEQLEPRRLFE
                     LFVDHRSQLGMDILGLSGATVGRQLATALADNRVVSLVSDRDLTGRGVEVTMFGRERK
                     LPAGPALLSISTGSPLVPSAAYQTPEGWRLVFRPPLTVEPSGSRRKDVVALTEALAAE
                     YERLISAAPSDWHVFQPGWPE"
     gene            9639..>9841
                     /locus_tag="VIX62_02930"
     CDS             9639..>9841
                     /locus_tag="VIX62_02930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5859221.1"
                     /translation="MRVALICPYAWDDPGGVQTHVRELAESLLARDHAVTVIAPVRDG
                     AAQPWVSPVGRPVDIPYNASNAP"
BASE COUNT         1455 a         3262 c         3444 g         1680 t
ORIGIN      
        1 acctcgactc gtccacgagc cacgatatcc tcgagctcct gcggcgctcg gtggaggagt
       61 acggccagac gatcgtgatg gtcacgcacg acgccagtgc ggccgggtac gccgaccggg
      121 tcgtgttcct cgccgacggg ctcatcgtcg atgagatgcg tgagccgacg gcagcatccg
      181 tcctggacag actgaagtcg ctggaaggct gaccctcatg cttcgcgcca ccctgaaagg
      241 gctcctcgcc cgcaagcgcc gtctggcgct cacggtgatc tcgatcgtgc tcggggtcgg
      301 cttcgtggcg gggacgttcg tgctcaccga cacgatgaac aaggcgttcg accagttgtt
      361 caccgacgcg gccgcgggct cggacgtggt ggtccgggcc gaacaggcct tcgctccgag
      421 cgcgaccgga ccggggagcg gcgcgatcga ggaacgcgat ccggtcccgg acacgttgtt
      481 gcccacggtc gaatcggtcc ccggggtcgc atcggcttcc ggcgacgtct cgggaagcgc
      541 gcaggtcgtc gacccggcaa cgggcgaccc gatcggcggc gtcggtccgc cgacgatcgg
      601 aacgaactgg accgacacga acccgaccgt gcagatccgg gagggcgcgg cgcccagcgc
      661 cgccgatgac gtcgtgatcg acgccgcgac ggcgacccgc tacaacctcg cggtgggtca
      721 ggacgtgaag atcctcttcc agggtcctcc cgaggagttc acgatctccg ggatcctcgg
      781 gttcggcgac gccgacaacc tgggtggtgc cacgctggcc gcgttcacct tgcccaccgc
      841 ccagcgggtg ctcgacaagg agggcgaatt cgattcgatc tcggtgctcg gggacgaggg
      901 cacggacccc gggcagctgc gggccgcgat ccaggacgtg ctgccgccca aggtcgaagc
      961 ggtcacgagc acatcagtgg ccgacgagca gtccgaccag ctcaaggaag ggctcggatt
     1021 cttccggatc gccctgctgg tcttcgcgtt catcgccctg ttcgtggggt cgttccagat
     1081 cttcaacacc ttttcgatca tcgtcgctca gcgggccaag gagctggccc ttctgcgcgc
     1141 cgtcggcgcg agccgccgcc aggtcctcac ctccgtgatc atcgaagccc tcgtgctcgg
     1201 actggtggcg gcgatcatcg gcatcgtcgc cggggtcggg atcgcggtgc tcctgaaggc
     1261 gttgctgtcc gggttcggca tcgacctgcc cagcacctcg ctccagctcg agccgcggac
     1321 catcgtggtc tcgctgatca tcggggtggt cgtgaccacc gtggcgtcgg tgcttcccgc
     1381 ccggcgggcc gcgcgcgtgg ccccgatcga ggcgctgcgt gactcgcagg acgccggcgc
     1441 cgaccacctg gggcggcgct cggtgatcgg cgtgatcgtg acggcgctgg ggctcgcgtt
     1501 cctgggctac gggctgttcg gggaacccga caacgccggt ctcctgatcg gcggcggggc
     1561 ggccgtgatc ttcatcggga tcgcgatcct gtcgcccctc gcggcccgtc cgctctcggg
     1621 cgcgatcggg cgcccggttc gcggcttgag catggcggcc cggttggggc gggagaacgc
     1681 gatgcgcaac ccacgccgga cggcggcgac gtcgtcggcg ctgatgatcg gcctggggct
     1741 gatcgccatg gtctcgatct tgtccgcgtc gctgaaggcc tcgttcgacg cggccctgca
     1801 ggacacgctg cgcgccgacc tcgtgttgac gtcgtcctcg ttcctcccgt tcagccagga
     1861 agtggcggct cggaccgccg acgtcgacgg tgtcgaggcc gtctcggcgt tccggcaggg
     1921 agggttcaag gtcaacgaca gcgacgccgc gatgaccggc gtggatccgg ccacgatcga
     1981 aaccgtggcg aacctgggac cgagcgaggg agccatcgcc tccctcgacg acggtggcgt
     2041 gctggtctac gaccagacga tggaggacaa cggatgggcc gtgggcgacg agctcccatc
     2101 ggcgttcgcc acgatcggcg acgatccgct cacgatcggc gggaccttcg acgacaatcg
     2161 gttggtgggg gactacgtgg tgtccctcga cacgttcgac gagctcttcc gcgcccagct
     2221 cgacacgttc gtgttcgtga aggtcgcgga cggagccgat atcgccgccg tccgaggcga
     2281 cgtcgagcag gccacggccg agttcggcaa catcgaggta caggaccagg cagccttccg
     2341 cgatcagcag gccggcttcg tgaaccagct gctgggtctc gtcaccgcga tgctgttcct
     2401 cgcggtcgtg atcgcgctct tcgggatcgc gaacacgctg agcctgtcga tcttcgaacg
     2461 cacccgggag ctcgggctgt tgcgcgccgt gggcatgggt cggacgcagg tgaagcgcat
     2521 gatccgatgg gagtcggtga tcatcgcgat cctcggggcg ttgttcggca tcgcgatcgg
     2581 catcttcttc ggctgggcgc tgcagcaggc gctggcgcct gagggcatca ccgagttcgt
     2641 gctgccggtg gggcagttgg tgttcttcct catcctcgcg gcgctggcgg gtgtcgtggt
     2701 cgcgctcctc cctgcccggc gagccgccaa gttgaacgtc ctcgaggcga tctcgtacga
     2761 gtaggcaggc gggcggtcgc tatcatggcc gccatgccca ccctgaccct tcccgacggc
     2821 gccgcgatcg agctccccga aggcgagccg atcggatccg cgctgcccaa gggcacgatc
     2881 gcggcacggg tggacggcga gctccgcgac ctctcgttcg tccccgccgc cgacgccgcg
     2941 gtcgaggcga tcgctccggg ctccgaggac ggattgcacg tactgcgcca ttccaccgcc
     3001 catgtgctgg cccaggccgt ctgcgatctc cacccgggga cgaagtacgc gatcgggccc
     3061 gcggtgaccg acggcttcta ctacgacttc gacctgcccg agacgatcgg caccgccgag
     3121 ctcgcgaaga tcgaccggcg catgcggcag atcgtgaagc agaaccagcc gttcgtccgc
     3181 gaggaggtct cgcgtgccga agcggccgag cggctggcgg atcagccgtt caagctggag
     3241 atcatcgccg gcctcgacaa cgacgacgat ccggaggctt ccgagggtga gaccgtcagc
     3301 ctgtatcgca acggcgagtg ggccgacctc tgcatggggc cacatgtgcc gcacaccggc
     3361 aagctgggcg ccttcaaact caccaacgtc gcgggtgcct actggcgcgg ggacgagaag
     3421 aacccccagc tgacccgcat ctacggcacc gcctgggcga ccaaggacga tctcgaggcg
     3481 cacctgcacc gcttggagga ggccgagcgg cgcgatcacc gcaagctcgg gctggagctg
     3541 gatctgttct cgttccccga cgagatcggg tcggggctcg cggtgttcca tccgaagggc
     3601 ggcttgatcc ggcgcctgat ggaggactac tcgcggcgtc gtcacgagga ggccggctac
     3661 gagttcgtga acacgccgca catcacgaag cgggagttgt tcgaaaccag cgggcacctg
     3721 gagtggttcg ccgacggcat gttcccgccg atggagcttg acggcggcac ggagtactac
     3781 ctcaagccca tgaactgccc gatgcacatc ctgatctacc gcagccgcac gcggagctac
     3841 cgggagctgc cgctgcgctt gttcgagttc gggacggtgt atcgctacga gaagtccggt
     3901 gtgatccatg ggctcacgcg ggtgcgcgga ctcacgatgg acgacgcgca catcttcacc
     3961 acgaaggagc agatgggcga ggagctgcgc tccctgctgg tgttcgtgct cgaccttctg
     4021 cgcgactacg ggctcgacga cttctacctg gaactgtcga cccggccgga ggacaaggcg
     4081 gtggggtcgg acgaggattg ggcagaggca acggaggcgt tgcgcctggc ggccgagtcg
     4141 atggacctcg agctggtgat ggacgagggt ggcggggcgt tctatgggcc gaagatcagc
     4201 gtgcaggcgc gtgatgcgat cggccgcacg tggcagatgt ccacgatcca ggtggacttc
     4261 cagttcccac agcgcttcga cctgcattac gccgggcccg acggtgagcg gcaccggccg
     4321 atcatgatcc accgggcgtt gttcggatcg gtcgagcggt tcttcggcgt gctcaccgag
     4381 cactacgcgg gcgcattccc gctgtggctc gcgcccgagc aggtgcgttt cgtcgcggtg
     4441 gccgaccgtc acgtcgagca cagcgaggag ctcgccgcca aggccaagga cgccgggttg
     4501 cgggccttcg tcgacgattc gaaggaatcg gtcggcaaga agatccgagc ggcgcaactg
     4561 atgaaggcgc cgtacacgct ggtgatcggc gaccaggagc tcgagtccgg cctctacacc
     4621 gttcgcgatc gcgcgggcac cgagcaccag ggtgtggagt tcgacgatct cgtcaccgcg
     4681 ctgctcgccg aagccgacag ccgggcgctc gagcagactg acttcggagc cgcctgagtg
     4741 gatcacctct ggagtccgtg gcggatggag tacatccgtt cgggttccga ggaacgtggt
     4801 gtcaccgacg gggcgtgcgt cttctgcgtc atcccggatc gtgaacccga gcgcgtgctg
     4861 gcgactggcg ccacggccta catcgtgttg aacaagttcc cctataaccc cgggcatctc
     4921 ctggtggtcc ccctccggca cgtgggcgat ctggaggagc tgagctccga tgaactcctg
     4981 gaggtgcaga tgttgatcca gcgatccgtc cgggcgctgc gcgaggatca gccgcacggg
     5041 ttcaacgtgg gactgaacct gggcgccggc gcgggtgcgg gcatccccga acacgtccac
     5101 tggcacgtcg tgccgcgctg gaacggcgac accaacttca tgtcggtcgt gggcgagacg
     5161 agggtgctgc ccgaactcct cgccgacacc gctcgccggc tcgcgccgag gttcgcagaa
     5221 tgagcaccgt gatggatggc accgtcgcgc ttcccgatgg cggccatctc gcctacgacc
     5281 tggcgggcga ggggccgatg ctcacgatga tccaccccgg actgtgggac cgacgcacct
     5341 gggatcggga gttcgacgcc tggaccgatc ggtaccgcgt gctccggtac gacgtccgag
     5401 ggtacgggcg gtccagccgt ctcacgggag cgtcctactc ccatgcgcgg gacctcgcgg
     5461 tgttgctcga gaacatgggc atcgagcaga cgagcatcgt ggggtgctcg atgggtgggg
     5521 cgatcgccat cgacttcgtg ttggtggaac cgaccaaggt cgtgagcctc gtgcttgcgg
     5581 ccgccgggct cggggggttc gaagccaacg aggaagagaa cgcgtggtgg gaggcacgga
     5641 tggatcccgt ggaagcggcg gtcgaggccg gtgacctgga gcgagcggag gacctgcgac
     5701 tcgaggtctg ggcgccgatg ggcacggacg atgccgccgg ccggcgcatc cgcgagatcg
     5761 cgttcgacaa catccacgaa ctgacgatgg atgagagcgc cgaggaagaa ctcgaacccc
     5821 cggccgccct gcggctgggc gagatcgacg tgccgacgct ggtcctggtc gccgagcacg
     5881 atccgccctt catgcggcgg tgtgacgagt tgatcggacg cgaggtgctg gacggccggt
     5941 tggtgaggat cgatgccgat cacgtcgtca acgtgcgcaa ccccgaagcg ttcgacgccg
     6001 cggtcctgcc cttcctggcc gagtacgcgc tctgagcgcg ttcgcgacgc ctacttcttg
     6061 tgggcttcct cgcggctctt ctggtagtcg tcgatgactt tctgggcgag gtgctgagga
     6121 agctgctcgt agtggctgaa ggtcatggag aaggcccccc ggcccccggt cattgaacgg
     6181 aggtcgatca cgtagcgcgc cacctcggcc tgcggcacct gggccttgat ccgctgcttg
     6241 ccgccaccga cctgctccat ccccgcgatc ttgccgcgct tggcgttgag atcgcccatg
     6301 acgtcgccgg tgtaggcctc cggcacgacg acctcgagat ccacgaccgg ttccaacagc
     6361 actaccccgg cctggtcgac cgcctccttc agcgccatcg accccgcgat ctggaaggcc
     6421 atgtctgagg agtccaccgt gtggaacttg ccgtcggtca gggtcacctt gatgtcgacc
     6481 atggggttcc cgctcaccac gccgtcgacc atcgtcttga cgatgccctt ctcgatcgac
     6541 gggatgaact gctgcgggat cgcgccgccg aagatcttgc tctcgtagac gaacccttca
     6601 ccgtggggga ggggctcgac gtcgatcacg cacacggcgt actggccgtg accaccgctc
     6661 tgtttgacgt gccggccggt ggcggtggcc ttggtgcgga tcgtttcccg gaaggcgatc
     6721 ttcgcgggac tggtgacgac gtcgaccccg aacttgccct tgagtcgctc gatcatcacc
     6781 tcgagatggg cctcgcccat cccgtgcatc acggtctcgt gcgtctcttc gctccggctc
     6841 acgcgcaagg tggggtcctc ttcacggagt cgagcgagtc cggtggcgag cttgtcctcc
     6901 tcgcccttgg ccttgggcgc gatcgcgtag gccagcagcg gctcggggag ctcgagcggt
     6961 cgcaggtgca ccggcgcgtc cttggtcgcg aacgtgtcgc cggtgtgggc gtgctgcagc
     7021 ttcgcgacgg cgccgatgtc cccggccgag accgctggga ccgattcgtg ctccttgcct
     7081 cgttgcgtga acagctgacc gatgcgctcg tcggtgccct gcgtgctgtt gtggaccgag
     7141 gtgtccggct ggatcgcccc gctgaagacc cggaacatcg tgatgtggcc gacgaacggg
     7201 tccgagaccg tcttgaacac gaacgcggtc gtcggcccgt tctcctcgct cggccgctcg
     7261 ctctcggtgc cgtccttgcc gatcaccgtg atcggaccgc gctccagcgg ggaggggaac
     7321 tcctccacga tgaagtccag gagccggtcg atgcccatgg ccttcgtggc tgatccgacg
     7381 atcaccggcg ccagccgcgc gcgggcgaag ccatccttgg cgccggacac gatgtgctcc
     7441 tccggcaggg ttcccacctc gaggtactcc tcgatgagcg cgtcgtcgga ctcggcgacc
     7501 gcttcgatca gcttctcgcg ggcggggtcg gccatggcgt gcaggtcatc gggccaatcc
     7561 gactcctcgg ccttcgggcc gctcggatac aggtcggcct tctcgtgcag cagatcggcg
     7621 acgcccttga actcgtgctc ctgaccgatc gggagttcga agggggccac ctggttgccg
     7681 aacgaggaca cgagttggtc gagcgtcgcc tggaagtcgg cgcgttcgcg atcgagcttg
     7741 ttcacgaaga tcgctcgcgg caggccttcc tcgaccgcca gctcccacgc ggcttcggtc
     7801 tggacctcga caccgtccac ggccgagacc acgacgatca cggcatccac cgcgcggatc
     7861 gccgagcgca cgtcgccgat gaagtcggca tagccgggcg cgtcgaggac gttgatcttg
     7921 accccgttcc attccaccgg ggccatcgcc agggagaccg agatgccctt gcgttgctcg
     7981 tcggggtcgt ggtcggagac ggtgttgccg tcctccaccg tgcccatgcg cgtgatcgcg
     8041 cccgaggtga agagcatggc ctcgagcagg gtcgtcttgc ccgaccctcc gtggccgacc
     8101 agcaggacgt tgcggatgtc cttggcgtcg taggtcttca acgcaactcc ttcgcccgag
     8161 aggtaggggg catcctactc aagttgctac gctccggcgg accctcatgc ttcgattcga
     8221 acgccaacca cccgggacgg ccctcacgcg gagcacgctg gtcgcggggg cgctcgtcgt
     8281 ggtcgccgcg gggtggttcg tggtggcccg ggaacccgag gtcgccgccg ccttcgctgc
     8341 cgccgcctcc gtcctgctcc tggtgggagg gcaccgcgcg aaccacggcg ccgggggacc
     8401 caccgaccgc atgctcgacg aactgctcga ccgtgcgtgg gacggtgtga tcctggggac
     8461 gatcgcatgg gtggcccgca tcgccacgcc gaccacggcg gtggccgcgt tggtggccct
     8521 gtgcgcgagc ttcctgtcgg cgtacgtccg ggcgcgggga gcatcgctgt cctacagcgt
     8581 cgaggaatcg cacatcacca gaggcatgcg ctacgcgctc gtgtgcgccg ggctcgcgtt
     8641 cggatggctg acctggacgc tgtgggtcgc cgccgcgatg tccgtgctgg ccgtcgtggt
     8701 gcggacctcg caggtggccc gggaggagcg cgcatgaggg ggtccgagga gaccccgaag
     8761 cagacggtga tcttccacgt ctaccgggcg gtcgcgtggc tgggccggac gctgcctgag
     8821 aggttcggcc ggcgcctgtt ccggttcggt gggctgctgg cgcacgatct gatgcccggt
     8881 gtgcgtcgaa ccgtggcctt caaccaggct caggtgctgg ggcggccgcc gggcgacccg
     8941 ctggtgaggg cctccacccg cgaggcgttc gacctctacg cgcgctactg gttcgactcg
     9001 ttccacgtcg tgggcgagac cgacgagacc atgatcgagc ggttcaccac ggtggacgcg
     9061 caccacatgt tcgatccgct cgaggccggg accggcgtga tcatcgccat gccccacgtc
     9121 ggagactggg atgcggcggg acgatggctc ttggcggaag gtcatcggtg cgtgtcggtg
     9181 gccgaacagc tcgagccccg gcgcttgttc gagctcttcg tcgaccatcg ctcacagctg
     9241 gggatggaca tcctcggtct gtccggggcc acggtggggc gccagctcgc gacggcgctg
     9301 gcggacaacc gcgtggtctc gctggtctcg gaccgtgacc tcaccggccg cggggtcgag
     9361 gtcacgatgt tcggacgcga acggaagttg cccgccggcc cggcgctgtt gtcgatcagc
     9421 accggatcgc cgctggtgcc gtcggccgcc taccagacgc cagagggatg gcggctcgtg
     9481 ttccgcccgc ccctgacggt ggaaccgagc gggagccgtc ggaaggacgt cgtcgcgctc
     9541 accgaggccc tcgcagcgga gtacgagcgg ttgatctcgg cggcgccctc ggactggcac
     9601 gtgttccagc ccgggtggcc ggagtagttc ggccgccgat gcgcgtcgcg ctgatctgcc
     9661 cctatgcctg ggacgacccc ggaggcgttc agacgcacgt ccgggaactg gccgagtcgc
     9721 tcctggcccg tgaccatgcc gtgaccgtga tcgccccggt tcgcgatgga gccgctcagc
     9781 cgtgggtgag cccggtgggc cgtcctgtcg acatcccgta caacgcctcg aacgccccga
     9841 t
//
LOCUS       DATDBZ010000118         4265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_449539, whole genome shotgun sequence.
ACCESSION   DATDBZ010000118 DATDBZ010000000
VERSION     DATDBZ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449539"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VIX62_02935"
     CDS             <1..1116
                     /locus_tag="VIX62_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEY5859222.1"
                     /translation="GVEHCPGPDTGAGDGGYTYGDLGEIIGFPEVHADGEIWVQALWD
                     LRTAIGTNVSERLVTRGMELSPPDPSFLDMRNAIIQADVVASGGTHVDEIWQVFARRG
                     MGYYASSISGDDVEVVEDFELPPDCAVQACGSISGTVTDSVNGDPIEGLTVAIAGLNT
                     AGGLSDVTDASGEYAIEDVPFHVYPSLVFDGSGYEPSVARNVKVVGDVVRDRSLTRDW
                     ASLEGGATLKSFTPPDYGPFCGTNANGAFDLSFGAGWPSDSVTNGSSGVSGPRKTVVQ
                     LPTAVDVSTFGVVSGGTCGDGPEAGVKAFTIETRKNANAPWRTAVTANAPSDGQLHAY
                     TPTAGTAGVRFVRFTMRSNHGDANFMDVLEVTVRGTT"
     gene            complement(1120..3228)
                     /locus_tag="VIX62_02940"
     CDS             complement(1120..3228)
                     /locus_tag="VIX62_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY5859223.1"
                     /translation="MSAAEPQISGPIDRVMVGTDRSETAERAVRWAATFADAFGAELH
                     IVQVVLAPNPGGTQYGAAEATRAAGAADDLGIYARQVAGDRGQAHVVLHDDPAMAIVE
                     AAEEHAIDVLVVGNAGMAGRKEFLLGNVPNRISHNARCTVIIVNTAAPDGSTTVQKAT
                     VRSGTAIRGSAADLHETEPRLMARGSHIAAVFAKHGMKELFGRPDEDGAVGRQRQAKR
                     LRSALEELGPTFAKLGQVLSTRPDLLPPEFIEELATLQDHVNPLSEQAVVQVMERELG
                     VPWEDVFETIDAKPLAAGTIAEVHRATLATGERVVVKVQRPNAREEIEQDLALLELFA
                     EKVSDRPGLNQVIDMGAVFEHLSTSLHRELDFRQEAGNIERMRGVIEDYDRLAVPDVY
                     GDLSSSRLLVMEEIQGVAISEAPEGSVRKEAARQLLESYYKQILVDGFFHADPHPGNL
                     MWWNDRIYFLDFGMVGEVDPEMREQLMLLLMAFWQEDVGFLTDVTLMMSGAIDRNDLD
                     IDAFRGEIGQLMGTYRTASLAEIQLGPVLQEMTEVSLRHGVPLPASLTLTGKALAQMQ
                     LATAALDPELDPFDVAGGFLMRSVFKEMGGKFDLKALFYQTQKFKVRALRVVEAVERL
                     IGARPGQKLEVNFRAATLEETIRRAGRRLALGLIAGAAVLASGLTGVSDRVAPWVPIT
                     FGVVGGLLTIAIVIDIIRKR"
     gene            3361..3432
                     /locus_tag="VIX62_02945"
     tRNA            3361..3432
                     /locus_tag="VIX62_02945"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3393..3395,aa:Thr,seq:cgt)
     gene            3495..4181
                     /locus_tag="VIX62_02950"
     CDS             3495..4181
                     /locus_tag="VIX62_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859224.1"
                     /translation="MKSARKLLATVFASLLLAFAGATAALAQTPIDPSATVTENESAT
                     APINPFGISISLAMILGLTGLLVVLIIIIRYMRFAPRFSQDEESMKVVRADRVVLGQE
                     LPRRNVDLSQAAPVLVAPPAVPTSLPAASTEAPAAAAASEPVAVAATAPAPAAAAASA
                     PAPAAAPAAAPAPAPAATEERPEVSLDQETFDATLKELLDAGTDRRIAEGKARRAAMI
                     AARKKAAGEA"
BASE COUNT          623 a         1525 c         1442 g          675 t
ORIGIN      
        1 ggcgtcgagc actgccccgg tccggacacg ggcgccggcg acggcggcta cacctatggc
       61 gacctcggcg agatcatcgg gttcccggag gtccacgccg acggggagat ctgggtgcag
      121 gcgctgtggg atctgcgcac ggcgatcggc acgaacgtgt cggagcgtct cgtgacgcgg
      181 ggcatggagc tctcaccgcc cgacccgtcg ttcctcgaca tgcgcaacgc catcatccag
      241 gccgacgtcg tcgcctcggg cggcacccac gtcgacgaga tctggcaggt gttcgcgcga
      301 cgcggcatgg gctactacgc gtcctcgatc agtggtgacg acgtcgaggt ggtggaggac
      361 ttcgagctgc cgccggactg cgcggtgcag gcatgcggct cgatctcggg cacggtgacg
      421 gactcggtga acggcgaccc gatcgaaggg ctcacggtcg cgatcgccgg gctgaacacg
      481 gccggcggtc tctccgatgt caccgacgct tcgggcgagt acgcgatcga ggacgtgccg
      541 ttccacgtgt atccgtcgtt ggtcttcgac ggttccgggt acgagccgag cgtggcgcgc
      601 aacgtgaagg tcgtcggcga cgtggtgcgt gaccggtcgc tcacccgcga ctgggcctcg
      661 ctcgagggcg gcgccacgct gaagtccttc acgccgccgg actacggccc gttctgcgga
      721 acgaacgcca acggcgcgtt cgacctgagc ttcggcgccg gatggccgtc ggactcggtg
      781 acgaacggca gcagcggcgt ctcggggccg cgcaagaccg tcgtgcagct cccgacggcg
      841 gtcgatgtct cgaccttcgg ggtggtctcg gggggcacct gtggcgacgg tcccgaagcc
      901 ggcgtgaagg cgttcacgat cgagacccgg aagaacgcga acgcgccgtg gcgcaccgcc
      961 gtcacggcga acgcccccag cgacgggcag ctccacgcgt acacgcccac cgcgggaacg
     1021 gccggtgtgc ggttcgtccg gttcacgatg cggtccaacc acggcgacgc gaacttcatg
     1081 gacgtcctgg aggtgacggt tcgcgggacg acgtagcgct cagcgcttgc ggatgatgtc
     1141 gatcacgatc gcgatggtga gcaggccgcc caccacgccg aacgtgatcg gcacccaggg
     1201 cgccacgcgg tcggacacgc cggtgaggcc gctcgccagg accgcggcgc cagcgatcag
     1261 tcccagggcg agccgccggc cggcgcggcg gatcgtctct tcaagcgtgg cggcgcggaa
     1321 gttgacctcg agcttctgac cgggtcgtgc gccgatcagc cgctccacgg cctcgacgac
     1381 gcgcagcgcc cgcaccttga acttctgcgt ctgatagaag agcgccttca ggtcgaattt
     1441 gccgcccatc tccttgaaca cgctgcgcat caggaacccg ccggctacat cgaaggggtc
     1501 gagctcggga tcgagtgcgg cggtggcgag ctgcatctgc gccagcgcct tgccggtcag
     1561 cgtgagcgag gccggcagcg gaaccccgtg gcggagcgag acctcggtca tctcctgcag
     1621 gaccggtccc agctggatct cggccagcga cgccgtgcgg taggtcccca tcagctggcc
     1681 gatctcgccg cggaaggcat cgatgtcgag atcgttgcgg tcgatcgcgc ccgacatcat
     1741 cagcgtcaca tcggtgagga agccgacgtc ctcctgccag aaggccatca gcagcagcat
     1801 cagctgttcg cgcatctccg ggtcgacctc gcccaccatg ccgaagtcca ggaagtagat
     1861 gcggtcgttc caccacatca ggttgcccgg gtgcgggtcg gcgtggaaga acccgtcgac
     1921 caggatctgc ttgtagtagc tctcgagcag ctgtcgcgcg gcctccttgc gcacgctgcc
     1981 ctcgggcgcc tcggagatcg cgaccccctg gatctcttcc atcaccaaca gccgcgagga
     2041 cgacagatcg ccgtagacgt cgggcacggc gaggcggtca tagtcctcga tcacgccgcg
     2101 catccgttcg atgttgccgg cttcctgccg gaagtcgagc tcgcgatgca gtgacgtcga
     2161 cagatgctcg aacaccgcac ccatgtcgat cacctggttg agcccgggtc ggtcgctcac
     2221 cttctcggcg aacagctcca gcaacgcgag gtcctgctcg atctcctcgc gggcgttggg
     2281 tcgctggacc ttgaccacca cccgctctcc ggtcgccagc gtggcccggt gcacctcggc
     2341 gatcgtgccg gccgcgagcg gcttcgcgtc gatcgtctcg aacacgtcct cccacgggac
     2401 acccagctcc cgctccatca cctgcaccac ggcctgctcc gacagcgggt tcacgtggtc
     2461 ctgcagcgtc gcgagttcct cgatgaactc ggggggcaac aggtcgggtc gcgtcgacag
     2521 cacctggccg agcttggcga acgtcgggcc gagttcctcc agcgccgaac gcagacgctt
     2581 ggcttgtcgc tgccggccca ccgcgccgtc ctcgtcgggg cgcccgaaca gctccttcat
     2641 cccgtgcttg gcgaacaccg cggcgatgtg gctgccacgc gccatcagcc ggggctcggt
     2701 ctcgtgcagg tcggcggcgc tgccgcggat cgccgtgccc gaccggacgg tcgccttctg
     2761 cacggtggtg ctgccatcgg gcgccgcggt gttcacgatg atcaccgtgc atcgagcgtt
     2821 gtggctgatc cggttgggaa cgttgcccag caggaactcc ttgcggcccg ccatgccggc
     2881 gttgcccacg acgagcacgt cgatggcgtg ttcctcggcc gcctcgacga tcgccatggc
     2941 gggatcgtcg tgcagcacga cgtgcgcctg gccgcggtcg cccgccacct gccgcgcgta
     3001 gatgccgagg tcgtcggccg cacctgccgc gcgcgttgcc tcggctgcgc cgtactgcgt
     3061 ccccccgggg ttcggcgcca gtaccacctg cacgatgtgg agctcggcgc cgaacgcatc
     3121 ggcgaacgtc gccgcccagc gcacggcccg ctcggccgtc tcggatcggt cggtgccgac
     3181 catgacgcgg tcgatggggc cggagatctg tggttcggct gcgctcatgg acgcatcatg
     3241 gccgttcggg ggcctgtgcg ccaaggaagt cgccccgtgg agcttgcggc gcaggcccga
     3301 acctgcggcg gggtgcctgc atccccgcga cgaaccccgt tgatagactc gcccgaaccc
     3361 gccggagtag ctcaggggta gagcaaccgc ctcgtaagtg gtaggtcatg ggttcaaatc
     3421 ccatctccgg ctccgtccat accgacacca cgcgggctcc gtgcgaccgc gggctggtgc
     3481 taggatgcgg cccgatgaag tcggctcgca agctgctcgc caccgtgttc gcctcgctgc
     3541 tgttggcctt cgccggcgcc acggccgcgc tcgcgcagac gcccatcgac ccctcggcca
     3601 ccgtgaccga gaacgagagc gccaccgcac cgatcaaccc cttcgggatc tcgatctcgc
     3661 tcgccatgat cctggggctc acgggcctgc ttgtcgtgct gatcatcatc atccggtaca
     3721 tgcgcttcgc gccccggttc tcccaggacg aggagtccat gaaggtggtt cgggccgatc
     3781 gggtggtgct gggacaggaa ctccctcgcc gcaacgtcga cttgtcgcag gccgctccgg
     3841 tgctcgtggc gcccccggcc gtgccgacgt cgctcccggc cgcctcgacc gaggcccctg
     3901 cggccgccgc cgcctcggag ccggtggccg tcgccgctac agcgcccgct cccgcagctg
     3961 ccgccgcatc cgccccagcg ccggccgccg ctcccgccgc tgccccagca ccggcccctg
     4021 ccgcgacgga ggagcggccc gaggtctcgc tcgatcagga gaccttcgac gcgaccctca
     4081 aagagctcct cgacgccggc accgatcgcc ggatcgccga gggcaaggct cgccgcgccg
     4141 ccatgatcgc ggcgcgcaag aaggccgccg gcgaagcctg agtcaccgat cgcgtcgatc
     4201 cacggcgcag taaggtcgtt ccatgagcgc cacccaggtg gattggtcca tgggttcccc
     4261 gtctg
//
LOCUS       DATDBZ010000119         3999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1881846, whole genome shotgun sequence.
ACCESSION   DATDBZ010000119 DATDBZ010000000
VERSION     DATDBZ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881846"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VIX62_02955"
     CDS             <1..491
                     /locus_tag="VIX62_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859225.1"
                     /translation="GPLEGRTFDIVYTGRGALCWLPDLKPWAKAVASFVKPGGIFYIH
                     EGHPVMWAVDDDQIEPNGLQLGYDYWGGETLTFPVEGSYADPEADVDADIEHGWNHSL
                     GEIVTVLASNGLRIELLDEKRVLDWPAGFLTKLDDSHYGFPPAQKGTIPLMYSLRARK
                     DP"
     gene            533..1516
                     /locus_tag="VIX62_02960"
     CDS             533..1516
                     /locus_tag="VIX62_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5859226.1"
                     /translation="MIEPTPVQGEATHAFRRLVDNVELVIAGNSEAVRTAAICLLADG
                     NLLLEGVPGVGKTTLARALARSIGGEFSRIQATPDLLPSDMTGISVYDQANGVFRFVP
                     GPVFANVVLVDEINRTTPRTQSALLEPMEERQVTVEGTTYPLPTPYVVIATENPVEQH
                     GTYPLPEGQLDRFAMTVQVGYPQGAMASDIVRRQLLDHPLRDLEAVLTPEDVLAAQRA
                     ARAVHVDDAVLDYVVDLVDATREAPEVALGASPRSTVALTRCAQARAVSEGRDYVLPD
                     DVKSLAPAVLAHRLIPRQARSGAEVGRRVVGRILDEVAIPLHVAGHGSGRG"
     gene            1518..2735
                     /locus_tag="VIX62_02965"
     CDS             1518..2735
                     /locus_tag="VIX62_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5859227.1"
                     /translation="MPRLRKRALSLLAGAGLLFFIGTNVQAGWLFVLAALILGAVAAG
                     WILPIRATSGVTISRRVPGRVHQGEETLVDLEIGNAGARGRRGLIVQDPHLDAVDLWV
                     GPIRAGERTDITTLRVAERRGRHGPDRVRVKSSAPFGVAERRRWVPVEGSTLVLPKVE
                     SLGALPFIEPAATTDHAIHSAPRRGQGPEYLGIREYRPGDSMRHVHWPSTARTGAVMV
                     REFEQEQTRRLAIVMDTSRDAGRTWTPLDRACSAAASLALAALAQGHGARIVTGSDGD
                     ADVLSRADEDEILERLALMEAGNRDGFAATVADLGPALRGVQTAVVVFPTWSANGPET
                     LPAALESLAEQIPRVVAVPIVVDARDTTHDSLDEVSIAGLEQRMRAAGASVYLWRVGE
                     TLAAALGQERPAP"
     gene            2732..>3999
                     /locus_tag="VIX62_02970"
     CDS             2732..>3999
                     /locus_tag="VIX62_02970"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HEY5859228.1"
                     /translation="MKLEIRKPSGPTEDSKAIRVVVAVAVEVAILAVVTQPGAVEPLT
                     AVASLLLAPLGYLFSYHRRGQANVILKLVLSVALLAALGQFIAEVRLATSVDQARLPL
                     ASLFLWVQVLHAFDVPRRRDLAFSMVSSLILMAEAGSLSLSTSFVVFLVPWAVLAGVW
                     LSLSSRPRPDQVTSPISVRRVAPPVKPPRLAPARSVAMTTTAALMAAFLLFLAMPRVP
                     GSLVKAPPFSLAGAPSPVNGFDGSVDNPGLPADPGDGVVDFASGAYPGFSDVVDLRAR
                     GQLSDELAFRVRATQAALWRAEVFDTYEGSRWTIEDDETEILPQDGDGTSYLVPPEVV
                     RRNGAYGEGAAHLTQTFYIDTPQPNVLFAAQGASQVYFPSAGLRVDAAGSIRSPILLD
                     QGMVYSVVSDVPVTSPSLLRRSGPVHVDRMT"
BASE COUNT          560 a         1361 c         1431 g          647 t
ORIGIN      
        1 ccgggccgct cgagggccgg accttcgaca tcgtctacac cgggcggggg gcgctctgct
       61 ggctgcccga cctgaagccc tgggcgaagg ccgtcgcgtc gttcgtgaag cccggcggca
      121 tcttctacat ccacgagggc catccagtga tgtgggccgt cgacgacgat cagatcgaac
      181 ccaacggtct gcagctcggg tacgactact ggggcggcga gacgctgacg ttcccggtcg
      241 agggttccta tgcggatccc gaagccgacg tcgacgccga catcgaacac ggctggaatc
      301 actcactcgg cgagatcgtg accgtgctgg catcgaacgg actccgcatc gagctgctcg
      361 acgagaagcg cgtgctcgac tggcccgccg gtttcctcac gaagctcgac gacagccact
      421 acggcttccc gccggcccag aagggcacga tccccctgat gtattcgttg cgagcccgca
      481 aagacccata ggcgcactca agcaaggctt ccgggccgtc gaagcttcca acgtgatcga
      541 acctacgccc gtacagggtg aggcgacgca cgcgttccgt cggttggtcg acaacgtcga
      601 gctcgtcatc gccggcaata gcgaagccgt ccgcaccgca gcgatctgcc tgctggcaga
      661 cggcaatctg ttgttggaag gggtgccggg ggtcggcaag acgacgttgg ctcgagcatt
      721 ggcgcgttcg atcggcggag agttcagccg catccaggcc acgcccgatc tgctgcccag
      781 cgacatgacg ggcatcagcg tgtacgacca ggcgaacggg gtgttccggt tcgtgccggg
      841 gccggtgttc gccaacgtgg tgctggtcga cgagatcaac cgcaccacgc cgcggacgca
      901 gtcggcgttg ctcgagccga tggaagagcg ccaggtcacg gtcgaaggca cgacctaccc
      961 gctgcccacg ccgtacgtgg tgatcgccac cgagaacccg gtggaacagc acgggaccta
     1021 cccgctgccc gaaggccagc tcgatcggtt cgccatgacc gtgcaggttg gctacccgca
     1081 gggcgcgatg gccagcgaca tcgtgcgtcg tcaactcctc gatcatccgc tgcgcgatct
     1141 cgaagccgtg ctgacgcccg aggacgtgct cgcggcgcaa cgcgcagcgc gagccgtgca
     1201 cgtggacgat gccgtgctgg attacgtggt cgacctggtc gatgccaccc gggaggcacc
     1261 cgaggtggcc ctgggtgcgt cgccgcgctc gaccgtcgcc ctgacccgtt gcgcgcaggc
     1321 gcgtgcggtg tcggaaggcc gtgactacgt gctgcccgac gacgtgaaat cgctcgctcc
     1381 ggcggtcctt gcccaccggc tgatcccgcg gcaggcgcgc agtggcgccg aggtgggacg
     1441 ccgcgtggtg gggcgcatcc tcgatgaggt cgcgatcccg ctccacgtgg ccgggcacgg
     1501 ctccgggcga ggctgacgtg ccacgcctgc gcaagcgggc gctgagcctg ctggccggtg
     1561 cgggcctgtt gttcttcatc gggaccaacg tgcaggcggg ctggttgttc gtcctggccg
     1621 ccctgatcct gggcgcggtg gccgccggat ggatcctgcc gatccgcgcc acgagcgggg
     1681 tgacgatctc gcgacgggtg cccggacggg tgcaccaggg cgaggagacc ctcgtcgatc
     1741 tcgagatcgg gaacgccggg gcccggggtc gtcgtgggct catcgtccag gacccgcacc
     1801 tcgatgccgt cgacctctgg gtcgggccga tccgcgccgg cgagcgcacc gacatcacca
     1861 ccctgcgggt cgcggagcgg cgcgggcgtc atggaccgga ccgcgtccgc gtgaagtcct
     1921 ccgctccgtt cggcgtcgcc gagcgccggc gatgggtgcc ggtggagggt tcgaccctcg
     1981 tgctgcccaa ggtcgagtcg ctgggggcgc tcccgttcat cgaaccggcc gctacgaccg
     2041 accatgcgat ccacagcgcg ccgcgtcgcg gccaggggcc cgagtacctg gggatccgcg
     2101 agtaccgccc cggcgacagc atgcggcacg tgcattggcc ctccaccgcg cgcaccggcg
     2161 cggtcatggt ccgtgagttc gaacaggaac agacgcggcg actcgcgatc gtgatggaca
     2221 cctcccggga tgccggccga acctggacac cgctcgaccg ggcgtgctcg gccgcagcct
     2281 cgttggcgtt ggccgccctc gcgcagggac atggggcgcg gatcgtcacc gggtccgacg
     2341 gggacgccga cgtcctgtcc cgagccgatg aggacgagat cctcgaacgg ctggcgttga
     2401 tggaggccgg taaccgtgac gggttcgcgg ctacggtggc cgacctcggc ccggcgctcc
     2461 ggggtgtgca gacggccgtg gtcgtgttcc cgacgtggtc cgcgaacggc ccggagacgc
     2521 tgccggcggc cctcgagagc ctggccgagc agatcccgcg cgtcgtggcg gtgccgatcg
     2581 tggtggacgc gcgcgacacc actcacgaca gcctcgacga ggtgtcgatc gccgggctgg
     2641 agcaacggat gcgcgccgcg ggggcctcgg tctatctctg gcgcgtgggc gagaccctgg
     2701 ccgcggcact cggacaggaa cggccggcgc cgtgaagctc gagatccgca agccgtctgg
     2761 acccaccgag gattcgaaag cgatccgcgt ggtggtcgcc gtcgccgtcg aggtggccat
     2821 cctcgcggtg gtgacccagc cgggggccgt cgagccgctc accgcggtcg cctcgctgct
     2881 gctggcgcca ctgggctacc tgttctccta tcaccgccga ggtcaggcga acgtcatcct
     2941 caagctcgtg ctctcggtgg cgttgctcgc ggcgctggga cagttcatcg ccgaggtgcg
     3001 cctcgcgacg tcggtggatc aggcgcggct accgttggcg tccctgttcc tgtgggtgca
     3061 ggtgttacac gccttcgacg tcccgcgccg ccgtgacctg gcgttctcca tggtgtcgag
     3121 cctgatcctg atggccgaag ccggatccct gtcgctctcc acgtccttcg tggtgttcct
     3181 ggtgccgtgg gcggtcctag cgggggtgtg gctctcgctc tcctcgcgcc cgaggccgga
     3241 tcaggtgacc tcgccgatct cggtgcgccg tgtggcgccg ccggtgaagc caccgcggct
     3301 ggcgccggcg cgatcggtgg cgatgaccac gaccgcggcg ttgatggcgg cgttcctgct
     3361 gttcctcgcg atgccgaggg tgccgggatc actggtgaag gctccgccgt tctccttggc
     3421 cggcgcgccg tcgcccgtga acgggttcga cggcagtgtc gacaatccag gcctgcccgc
     3481 ggatccgggc gacggcgtgg tcgatttcgc ctccggggct tatccgggat tcagcgacgt
     3541 ggtcgatctg cgggctcgtg gacagctgag cgatgagctc gcgttccgcg tgcgagccac
     3601 gcaggccgcg ctgtggcgcg ccgaggtgtt cgacacctac gaggggagcc ggtggacgat
     3661 cgaggacgac gagaccgaga tcctgcctca ggacggggac ggcacctcct acctcgtgcc
     3721 gcccgaggtg gtgcggcgca acggtgcgta cggtgagggg gcggcgcacc tcacgcagac
     3781 gttctacatc gacacgccgc agcccaacgt gttgttcgcc gcccagggcg catcgcaggt
     3841 gtacttcccg tcggcgggcc tccgggtcga cgccgccggg tcgatccgtt cgccgatcct
     3901 gctcgaccag ggcatggtct attcggtggt ctccgatgtg ccggtcacct caccgagcct
     3961 gttgcgccgt tcgggcccgg tgcacgtcga ccggatgac
//
LOCUS       DATDBZ010000120         4384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1679836, whole genome shotgun sequence.
ACCESSION   DATDBZ010000120 DATDBZ010000000
VERSION     DATDBZ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679836"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..641
                     /locus_tag="VIX62_02975"
     CDS             27..641
                     /locus_tag="VIX62_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5859229.1"
                     /translation="MKLTATEQISVDGVMQGPGGPEEDERGRFERGGWAMPFTDDQTM
                     AFINEVYERADAFLFGRVTYEIFAGSWGTWDDPGDSPIWTALNTRPKYVASTTPFDPV
                     WANTTVLSGDVAAAIRGLKAEAGGELLVPGSGVLVRWLLDNDLVDEIQLLTFPVVVGE
                     GTRLFPDSGPDMALELVGSQAVASTGVTIQVYRPAGRPQYGAVT"
     gene            complement(752..841)
                     /locus_tag="VIX62_02980"
     tRNA            complement(752..841)
                     /locus_tag="VIX62_02980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(805..807),aa:Ser,seq:cga)
     gene            complement(888..1376)
                     /gene="tadA"
                     /locus_tag="VIX62_02985"
     CDS             complement(888..1376)
                     /gene="tadA"
                     /locus_tag="VIX62_02985"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY5859230.1"
                     /translation="MDQTSSLDERSLDERHMEAALEAARACTEHGDVPVGCVIVRDAD
                     AKVVATAGNGRERLQDPTAHAEILALRDAATTAGSWHLEDHILVVTLEPCAMCAGAIV
                     LARIRRVVFGASDPKAGFAGSLDNLVDDPRLNHRVEVSSGVLAEECGDLLRTFFRDRR
                     TP"
     gene            complement(1378..1731)
                     /locus_tag="VIX62_02990"
     CDS             complement(1378..1731)
                     /locus_tag="VIX62_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEY5859231.1"
                     /translation="MPPLDQDRFMALVAEAVDSLPAWVHDTLDNVEILIDDEPPSGQR
                     NLLGLYEGIPLTARGDNYTGVLPDKITLFAGPLWREARGDEDRLRAAVRHTVAHEVAH
                     HFGISDERLLEIDAY"
     gene            1749..3086
                     /locus_tag="VIX62_02995"
     CDS             1749..3086
                     /locus_tag="VIX62_02995"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY5859232.1"
                     /translation="MPGGLLTDLYELNMAASYLRRDMDGLATFSLYVRGLPPQRGFLV
                     AAGLESCLDFLASFSFTDDDLTTLGGMGFDDRALTDLAALRFDGEVWAVPEGTIVFSE
                     EPLLEVTAPVAVAQIVETYLLNQITLHTTLASKAARYVLAADGHDLVDFAFRRTHGTE
                     AAMAIARASAMVGFVATSNVEAARRFGLTVAGTMAHAFIEAFPSEREAFEAFAEDHPD
                     RATFLVDTYDTEQGVRTAIATIEALGLSERIGVRLDSGDLDALSRTARSLLDAAGLPQ
                     ARVFASGGLDEHEVAALIGGGAPIDAFGIGTQMGVSADAPFVDSVYKLTEFDGRPTLK
                     LSSGKASLPGRKQVWRTVAGGEHRDTLGLRDEVGPPETRPLLEPVMRDGRPLAPPPSI
                     GDALRTFQAELAQVPEAARRLEHPEQVEVARSSALLELTEETRAAALTKAEIG"
     gene            3132..4160
                     /locus_tag="VIX62_03000"
     CDS             3132..4160
                     /locus_tag="VIX62_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859233.1"
                     /translation="MRNRAAIAALLSLLMLVATLPASAGPSVTIDPVKTAAKTEFLPA
                     PGTSVTHGYFAWSSPVNGRSHVFVEVDGGPKSQIDNGVYGFAGAFAAGEDNLIFQQIK
                     FPGGDAQSDLRMWDGLTGDTNAFVSNINTDQWQWAPSYDLDGSDLWLLYGENRFNGPK
                     APWKIILFNGSMGTKQVLAETTYRCGCIFPGTFAYPWVSWSKGLDGAVWRMNVTTGIK
                     EKLTVPGNRDEHSIGVTADGTAYVVQDGAACGTHSKIFRIDPDGTATLIASMPAGTEA
                     YTLAPFDTGTGIDVYFDRYKCSTKRYDIYVIRDAEAVARTAPVHLGSGGGAPAGGRRF
                     PVGSVPPR"
     gene            4203..>4384
                     /locus_tag="VIX62_03005"
     CDS             4203..>4384
                     /locus_tag="VIX62_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859234.1"
                     /translation="MSEPTRRRRRWPGRLLIGSLIAAAIFYGGGGVYFSNVLNERALD
                     GASRRKDTKLLEPDVEV"
BASE COUNT          702 a         1510 c         1498 g          674 t
ORIGIN      
        1 actgcaccac gagagagagg acgaccatga aactcacagc caccgaacag atctcagtag
       61 acggagtgat gcaggggccc ggcggtccgg aagaggacga gcgcggccga ttcgagcgcg
      121 gcggatgggc catgccgttc accgacgacc agaccatggc gttcatcaac gaggtctatg
      181 agcgcgccga cgcattcctg ttcggccgag tgacctacga gatcttcgcc ggctcctggg
      241 gcacgtggga cgatccgggc gacagcccga tctggacggc gttgaacacc cggcccaagt
      301 acgtggcatc gaccacgccc ttcgacccgg tttgggcgaa cacgaccgtc ctctccggcg
      361 acgtcgcggc cgccatccgc ggattgaagg ccgaggcggg aggcgagctg ctggtgcccg
      421 gcagcggcgt cctggtccgg tggctgctcg acaacgacct cgttgacgag atccagctgc
      481 tcaccttccc cgtggtcgtc ggcgagggca ccagactgtt ccccgactca ggccccgaca
      541 tggcgctcga gctggtcgga tcgcaggcgg tcgcctccac aggagtgacg atccaggtct
      601 accggcccgc tgggcgcccg cagtacggcg cggtcacgtg acgtcgatcg cgcatcctcg
      661 cgcgtgagtc cctgatcctg ccctccgacc tcggccgagt cgcgatcctt cgcgtactcc
      721 ggcgtaccgg ccgaggtcgg aggtatggaa gcggaggcgg cgtgattcga acacgcgagg
      781 ggcggtttag acccctaacc gctttcgagg cggtcccgtt cgtccgctcc ggcacgcctc
      841 cgtcgccgat tctagcaatc gctttcggac ctggcccaac aagaaggtca cggcgtgcgt
      901 cggtcgcgga agaacgtgcg cagcaggtct ccgcactcct cggcgagcac cccggacgag
      961 acctccacgc gatggttcag ccgcggatcg tccaccaggt tgtcgagcga accggcgaac
     1021 cccgccttgg ggtcggaggc cccgaacacc acgcggcgga tgcgcgcgag cacgatggcg
     1081 ccggcacaca tcgcgcacgg ttcgagcgtg accaccaaga tgtgatcctc caggtgccac
     1141 gagcctgctg tggtcgccgc gtcgcggagg gcgaggatct cggcgtgcgc cgtcggatcc
     1201 tggaggcgtt cacggccgtt gccggcagtg gccaccacct ttgcgtcggc gtctcgcacg
     1261 atcacgcacc cgacgggcac gtcaccgtgc tcggtgcagg cgcgagcggc ctcgagtgcc
     1321 gcctccatgt gccgttcgtc cagggaacgt tcgtccaagg aactagtctg atccatacta
     1381 gtaggcgtcg atctcgagca gccgctcgtc gctgatcccg aagtggtggg ccacctcgtg
     1441 ggcgaccgtg tggcgaaccg cggcgcgcag gcggtcctcg tcgccacggg cttcgcgcca
     1501 cagcgggccc gcgaacagcg tgatcttgtc gggcagcact ccggtgtagt tgtcccctcg
     1561 cgccgtcagc gggatgccct cgtacaggcc caggaggttc cgctggccgg agggtggttc
     1621 gtcgtcgatc aggatctcga cgttgtcgag cgtgtcgtgc acccaggcgg ggagcgaatc
     1681 gacggcctcg gccacgagcg ccatgaagcg gtcctggtcg aggggcggca tggcggtagc
     1741 ctacgcgcat gccgggtggc ctcctcaccg atctctacga gctgaacatg gcggcgagct
     1801 acctgcgccg cgacatggac gggctcgcga ccttcagcct gtacgtgcgc ggattgccgc
     1861 cgcagcgcgg gttcctggtg gcggccggac tcgagtcctg cctggacttc ctggcctcgt
     1921 tctcgttcac cgacgacgac ctgacgaccc tcggcgggat gggcttcgac gaccgcgcgc
     1981 tcaccgatct ggcagcgctg cggttcgacg gcgaggtgtg ggcggtgccc gagggcacga
     2041 tcgtcttctc cgaggagccg ctgctcgagg tcacggcgcc ggtcgcggtg gcccagatcg
     2101 tcgagaccta cctgctgaac cagatcacgc tgcacaccac gctcgcatcg aaggccgcac
     2161 gctacgtgct ggccgccgac ggccacgatc tcgtggactt cgccttccgg cgtacgcacg
     2221 gcaccgaggc ggcgatggcc atcgcacgcg ccagcgccat ggtggggttc gtcgccacgt
     2281 cgaacgtcga ggcggcacga cggttcggcc tgacggtggc gggcacgatg gcccacgcgt
     2341 tcatcgaagc cttcccgagc gaacgtgaag ccttcgaggc gttcgccgag gatcaccccg
     2401 accgcgcgac gttcctcgtc gacacctacg acaccgagca gggtgtccgc accgcgatcg
     2461 ccacgatcga ggcgctcgga ctcagcgagc ggatcggcgt gcgcctcgac agcggcgacc
     2521 tggatgcgct gtctcgaacc gcgcgcagct tgctcgacgc cgccggtctc ccgcaagcga
     2581 gggtcttcgc cagtggcggg ctcgacgagc acgaggtcgc ggcattgatc gggggcgggg
     2641 cgccgatcga cgcgttcggc atcgggaccc agatgggcgt gtcggcggac gcgccgttcg
     2701 tcgactcggt gtacaagctt acggagttcg acggccggcc cacgctgaag ctctcctcgg
     2761 ggaaggcctc gctgcccgga cgcaagcagg tctggcgcac ggtcgccggc ggggaacatc
     2821 gcgacactct gggcctgcga gacgaggtgg ggcctccgga gaccaggccc ttgctcgaac
     2881 ccgtgatgcg cgatggacga ccgctcgcgc cgccgccctc catcggcgac gcactgcgaa
     2941 ccttccaagc tgaactcgcc caggtgcccg aggccgcccg acggttggaa catcccgagc
     3001 aggtcgaggt cgcacgctcc tcggcgttgc tcgagctgac cgaggagacc cgcgccgcgg
     3061 ccctcacgaa ggccgagatc ggctgaccgt cctgcgttga cagggccgtg tcgcgacccc
     3121 aggatgcctc catgcgaaac cgagccgcca tcgccgccct cctgtcgctg ctgatgctgg
     3181 tcgccacgct ccccgcctct gcggggccaa gcgtgacgat cgacccggtg aagaccgccg
     3241 ccaagaccga gttcctcccc gcgcccggca cgagcgtcac ccatggctac ttcgcgtgga
     3301 gctcgccggt gaacgggaga tcgcacgtct tcgtcgaggt cgacggggga cccaagtccc
     3361 agatcgacaa cggcgtgtac ggattcgcgg gagcgttcgc cgcaggcgag gacaacctca
     3421 tcttccagca gatcaagttc cccgggggag acgcgcagtc ggacctgcgt atgtgggacg
     3481 gcctcaccgg cgacacgaac gccttcgtga gcaacatcaa caccgatcag tggcagtggg
     3541 cgccgtcgta cgacctcgac gggtccgacc tgtggctgct gtacggcgag aaccgattca
     3601 acgggcctaa ggcaccatgg aagatcatcc tgttcaacgg aagcatgggc acgaagcagg
     3661 tgctcgccga gacgacgtac cggtgcggct gcatcttccc ggggacgttc gcctatccgt
     3721 gggtgtcctg gagcaagggc ctcgacgggg cggtctggcg gatgaacgtc acgaccggca
     3781 tcaaagagaa gctgacggtc ccgggcaacc gtgacgaaca ctcgatcggg gtgaccgccg
     3841 acggcaccgc ctacgtcgtg caagacggcg ccgcgtgtgg cacgcacagc aagatcttcc
     3901 gcatcgaccc cgacggcacg gcgacgctga tcgcctcgat gccggccggc acggaggcgt
     3961 acaccctggc gccgttcgac accgggacgg ggatcgacgt gtacttcgat cgctacaaat
     4021 gctcgacgaa gcgctacgac atctacgtga tccgggacgc cgaggcggtc gcccgcaccg
     4081 ctccggtgca cctcggttcc ggcggcggcg ctccagcggg cggccggcgg ttccctgtgg
     4141 ggtcggttcc gccccgctga tcgttcgcgg gtcgagcccg ccgcggcacg taccatgagc
     4201 gcgtgagcga accgacgcgc cgacggcggc gctggcccgg ccgtctgctg atcggctcgt
     4261 tgatcgccgc cgcgatcttc tacggcggtg gcggcgtcta cttctccaac gtgctgaacg
     4321 aacgcgcgct ggatggcgcc tcgcgccgca aggacacgaa gctcctggag cccgacgtcg
     4381 aggt
//
LOCUS       DATDBZ010000121         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1045555, whole genome shotgun sequence.
ACCESSION   DATDBZ010000121 DATDBZ010000000
VERSION     DATDBZ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045555"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..575
                     /locus_tag="VIX62_03010"
     CDS             249..575
                     /locus_tag="VIX62_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEY5859235.1"
                     /translation="MQTILDFDRVFSALADATRRDIVRRAIDGDEGVAELADHYPMSF
                     AAVQKHVAILERAGMVTKQRIGRRKVVRTNLAGLAVARRLLDQYEELWRGRIGRMTDL
                     VEATKE"
     gene            581..1096
                     /locus_tag="VIX62_03015"
     CDS             581..1096
                     /locus_tag="VIX62_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009738759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5859236.1"
                     /translation="MTVTAIRKDPESLTMTLDAEFDASPDRVWQLWADPRQLERWWGP
                     PTYPATFTKHELSPGSRIEYHMTGPEGDQPRGFWDVLEVEPPRRLLVRDGFANDDGTP
                     NTHMPTGLQEVTIEEIDNGRTRMSINSVFPSTEAMEQLIAMGQEEGLTQAVGQIDAIL
                     AEDAVGAGQGH"
     gene            1154..1759
                     /locus_tag="VIX62_03020"
     CDS             1154..1759
                     /locus_tag="VIX62_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5859237.1"
                     /translation="MSDLVIDFITSLDGYASAEGWPGWWGLEGPEYLAWLGERPDRDY
                     TLLMGANTYRLMYSFAAEPESSNPNATAEEDAATSELTKLPKVVFSSTLAAPLPWANT
                     RLVTGDAVDAVAAMKREGTNMSTIGSLSLCRSLLKAGLVDRYRVVMFPVITGSTGRER
                     IYDGYPDVALEMIDSRTFDGRIQLVEFVPTLLTGPPGAAVS"
     gene            1852..>2367
                     /locus_tag="VIX62_03025"
     CDS             1852..>2367
                     /locus_tag="VIX62_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5859238.1"
                     /translation="MTIAEATTRTVEVPGATLTYDVRVNDASTEPPLFLIGSPMAAAG
                     FGTLAGHFADRTVITYDPRGSERSTKADPMSRSTPEDHAEDLHRIIQEVGGGPVDLFA
                     SSGGAVNALALVSKYPEDVRALVAHEPPLASQLPDRENAMAAARAINATYERSGWGAG
                     MAHFIAVVSHRG"
BASE COUNT          405 a          821 c          787 g          354 t
ORIGIN      
        1 cgcggcgctg tcgatagtgg cgacgaccgc gccggggaag gcgtcgcgaa tctgctggat
       61 catctcgtcc tgggtggggc cgtcaggcag tcgtgcatcg gtcatctctc gagactaccg
      121 actgcatgtc aagcccccac cgaacggggc atccgagagg ccgcgtgtca cactcggggc
      181 ggctggcgca tctcagcgct cggcgaaccg gcttgacagc acgcatatac aaccgtatgg
      241 ttgtatcaat gcagacgatc ctcgacttcg atcgcgtgtt cagcgccctg gcggacgcca
      301 cccggcgtga catcgtgcgc cgcgcgatcg acggggacga gggagtcgcc gagctggcgg
      361 accactaccc catgagcttc gcggcggtgc agaagcacgt cgccatcctc gaacgggccg
      421 gcatggtcac gaagcagcgc atcgggcggc ggaaggtcgt ccgcacgaat ctcgccggcc
      481 tggcggtggc ccgccgcctg ctggaccagt acgaggagct gtggcgcggc cggatcggcc
      541 gtatgaccga cctcgtcgaa gccacgaagg agtaaccccg atgaccgtca ccgccatccg
      601 caaggacccc gagtccctga cgatgaccct tgatgccgag ttcgatgcct cgcccgaccg
      661 ggtgtggcag ctgtgggccg acccacgcca gctggagcgg tggtggggcc ccccgaccta
      721 tcccgccacg ttcacgaagc acgagctctc tccgggcagc cgcatcgagt accacatgac
      781 cgggccggag ggcgatcagc cgcgggggtt ctgggatgtt ctcgaggtgg agccgccccg
      841 ccgcctcctg gtgcgagacg ggttcgccaa cgacgacggc acgccgaaca cccatatgcc
      901 aaccggactg caggaggtga cgatcgagga gatcgacaat gggcggactc gaatgtcgat
      961 caattccgtc ttcccgagca ccgaggcgat ggagcagctg atcgccatgg gccaggagga
     1021 aggcctcacg caggccgtcg ggcagatcga tgccatcctc gccgaggatg cggtcggagc
     1081 cggtcagggt cactgacgcg gcccgctctc ccgccggcca ctcgccggga cctcaacgcc
     1141 aagggaaatc cacatgtcgg acctggtcat tgatttcatc acctcgctcg acggctacgc
     1201 gtccgctgag ggctggccgg gatggtgggg cctcgagggg cccgagtatc tcgcctggct
     1261 cggcgagcgc cccgatcgcg attacacgct cttgatgggc gcgaacacct accggctcat
     1321 gtactcgttc gccgccgagc ccgagtcatc gaatccgaac gcgacggccg aggaggacgc
     1381 cgccacgtcc gagctcacca agctgcccaa ggtcgtgttc tcctcgacgc tggcggctcc
     1441 cctgccgtgg gccaacaccc ggctggtcac cggggacgcc gtcgacgccg tcgcggccat
     1501 gaagcgagag ggaaccaaca tgagcacgat cggcagcctc agcctctgcc gatcgctgct
     1561 caaggcgggt cttgtcgacc ggtatcgggt ggtgatgttc cccgtgatca cgggcagcac
     1621 cggacgtgag cgaatctacg atggctaccc cgatgtcgcc ctcgagatga tcgacagccg
     1681 gaccttcgac ggacgcatcc agctcgtcga gttcgtgccc accctgctga ccgggccccc
     1741 tggcgcggcc gtgtcctgag agctgattcc gcgccggccg atgagtccgg tacccgacga
     1801 gcagtcctac caccacccga cgacgcgcac cgaaccacag ggagacgacc gatgacgatt
     1861 gccgaggcga ccacccgcac cgtggaggtg ccgggcgcca cgctcaccta cgacgttcgg
     1921 gtcaacgacg ccagcaccga gccgccgctg ttcctcatcg gctcgccgat ggccgccgcc
     1981 gggttcggca cgctggccgg ccattttgcc gatcgcacgg tgatcacgta cgacccgcgc
     2041 ggctcagaac ggagcacgaa ggccgatccg atgagccgct ccacgcccga ggaccacgcc
     2101 gaggatctcc atcggatcat ccaggaggtc ggcggtggcc cggtcgatct cttcgccagc
     2161 agcggtggtg cggtgaacgc gctcgccctg gtgtcgaagt accccgagga cgtccgcgcg
     2221 cttgtggcgc acgagcctcc gctggcatcg cagctgcccg atcgcgagaa cgcgatggcg
     2281 gcggcgcgcg cgatcaatgc cacgtacgag cgcagcggct ggggcgccgg gatggcccac
     2341 ttcatcgccg tcgtcagtca tcggggc
//
LOCUS       DATDBZ010000122         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_450722, whole genome shotgun sequence.
ACCESSION   DATDBZ010000122 DATDBZ010000000
VERSION     DATDBZ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450722"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VIX62_03030"
     CDS             complement(<1..432)
                     /locus_tag="VIX62_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859239.1"
                     /translation="MKIPWPVRSVIAGAAGTTAMSLAYGSERRLRHATTQLDYDDSLV
                     PGQIVAGVLHLPNVTDRGDAELGSALRWGYGSAFGLWHGTLRKVMPEPWAALVFGATL
                     MTATFTLFPILGKTPPPWRWPSDVLLTAVGTHAAYVVAVAVV"
     gene            720..>2647
                     /locus_tag="VIX62_03035"
     CDS             720..>2647
                     /locus_tag="VIX62_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859240.1"
                     /translation="MLLVLAIPLIAALSFAAPGPGALDTAVVNLVTSLPGLIGWLWEI
                     SYDLLFGWALFVLAASLFAHARKRLLFGELLAAALAYLFAVGAGEIAGTGISDGLRGL
                     ASSEPPSVYPAMRLAVAAAVIVAASPHLSRPLRNTGRLVVTVGAIASIVLGIALPIGV
                     IAGLGVGFAAASVVHLLLGSPGGRLSLQQVGKALAELGIDATDLRYAPLEPSGVSLVL
                     ASGADDGSLLVKIYGRDAWDGQLITSVWSSLWNRGQTPQFGGRLHQVEHEAFVTLLAE
                     RAGVAVMPVVAADMADGRDAVLVVDTRDARPLVTLDPDEVDDALLTRIWEMDAQLFDL
                     GIAHGALDGYRVAVRDDGTPVLGDLSQATVSASDTALRSDRAQLLVSTALIVDHQRAV
                     EVAKSVIGADAIAEMLPYLQPAALDRTTRHAVRAQDWDLDDLRTMAADAAGVELPKLE
                     QLRRVSVARIVMLAVFGLITYVVISAVANVGLDSLIEEFQDADLIWLGLALMLAPVVA
                     FPQAFSTIGACIRPVRFGPVVMLQYAVQFVALAVPSSAARIALEIRFFTKVGLSSAGA
                     VAVGVIDSVCGFIVQMLFIIVIMLSGLATLDLSDAKGSPPTFTGKWLLIGGIACVLLV
                     IVLLAIPRFRAFAKGKAS"
BASE COUNT          366 a          909 c          890 g          482 t
ORIGIN      
        1 gacgacggcg acggcgacca cgtacgcggc atgggtcccc acggcggtga gcaggacgtc
       61 ggagggccac cgccacggcg gtggcgtctt gccgaggatc gggaacagcg tgaacgtcgc
      121 cgtcatcagg gtggcgccga acaccaacgc ggcccacggc tccggcatga ccttgcgcag
      181 ggtgccgtgc catagcccga acgccgaacc atagccccac cgaagcgccg aacccagttc
      241 tgcgtcccca cgatcggtga cgttcggaag atgcaggacg ccggcgacga tctgccctgg
      301 caccaaactg tcgtcgtagt cgagttgggt cgtcgcgtgc cggaggcgcc gctcggatcc
      361 gtacgcgagg ctcatcgcgg tggtaccggc agcgccggcg atcacgctgc gaacgggcca
      421 tgggatcttc atgatgtcca tccttccatc ctgggcctat ccgtggccat cgctgacgcg
      481 gtctgcagtg cgtttcgcgc ctgctggacg cagggtgtac cagaagctag cctccggcca
      541 cgggctgaga acgcgggtgg gggcgccaag gcatgacacg gacagagtcc gaggcaccga
      601 ctgaccctgc gagcgcaggc catcgtctgc gccggaaacc actcacgctg cgaaccgctg
      661 agatccgcgt gttctcgtcg gcgagtgatg cgccacgagc gagacgtccc accgacatcg
      721 tgctgttggt cctcgcgatc ccgctcatcg ccgctctgtc gttcgcggcc cccgggcccg
      781 gtgccctcga tacggccgtc gtcaacctcg tcacgtccct ccccggcctc atcgggtggc
      841 tctgggagat ctcctacgac ctgctgttcg gctgggcgct cttcgtgctc gcagcgtcgt
      901 tgttcgcaca cgcacgcaag cgcctgctct tcggcgaact gctcgcagca gcgctcgcct
      961 atctgttcgc ggtgggggcc ggagagatcg cgggcacggg gatctccgat ggactccgag
     1021 ggctcgcctc gtccgaacct ccttcggtct atccggcgat gcgcctcgcc gttgccgcgg
     1081 ccgtgatcgt cgcggcgtct ccccacctgt ctcgaccgct gcggaacacc ggcaggctcg
     1141 tcgtcacggt cggggcgatc gcgagcatcg tcttggggat cgccctgccc atcggcgtga
     1201 tcgccggcct cggtgtgggc ttcgccgccg cgtcggtggt ccacctgctc ctgggctcgc
     1261 cggggggcag gctctcgctg cagcaggtcg ggaaggcact cgccgagctc gggatcgacg
     1321 cgaccgatct gcggtacgcg cccctggaac cgagcggggt ctcgctggtc ctggcatcag
     1381 gtgctgacga cggatcgctg ctcgtgaaga tctacggccg cgacgcatgg gacggccaac
     1441 tcatcacctc ggtctggtcc tcgttgtgga accgtgggca gacgccccag ttcggcggtc
     1501 gcttgcatca ggtcgagcac gaggccttcg tgaccttgct cgctgagcgt gcgggggtcg
     1561 ccgtcatgcc ggtcgtcgcc gccgatatgg ccgacggccg ggatgcggtc ctcgtggttg
     1621 atacccgcga cgcgcgcccg ttggtcacgc tcgaccccga cgaggtcgac gatgccctgc
     1681 tcacgcgcat ctgggagatg gatgcccagc tcttcgacct cgggatcgcc cacggcgccc
     1741 tcgacgggta ccgggtcgcg gtccgggacg acggcacacc cgtcttggga gacctttccc
     1801 aggcgaccgt gtcggcctcg gataccgcgc ttcgctcgga tcgcgcgcag ctcttggtct
     1861 cgacggcgct gatcgtcgat caccagcgtg cggtcgaggt cgcgaagtcg gtcatcggtg
     1921 ccgacgcgat cgccgagatg ctcccctacc tgcaaccggc tgctctcgat cgcaccacga
     1981 ggcacgcggt tcgcgctcag gactgggacc tcgacgactt gcggacgatg gccgcggacg
     2041 ctgccggcgt ggaactaccc aagctcgagc agctcaggcg cgtcagcgtg gcccgcatcg
     2101 tcatgttggc agtgttcggc ctgatcacct acgtcgtcat ctcggcggtc gcgaacgtcg
     2161 gcctcgacag cctcatcgaa gagttccagg acgccgacct gatctggctc gggctggccc
     2221 tgatgctggc acccgtggtg gcgttccccc aagcgttctc gacgatcggt gcctgcatcc
     2281 ggccggtgcg gttcggtccg gtcgtcatgc tccagtacgc ggttcagttc gtcgccctcg
     2341 cggtaccgag ttcggcggct cgcatcgcgc tggagatccg cttcttcacg aaagtgggtc
     2401 tgtcatcggc gggcgccgtc gcggtcggag tgatcgacag cgtgtgcgga ttcatcgtgc
     2461 agatgctgtt catcatcgtg atcatgctgt cggggttggc gaccctggat ctctcggacg
     2521 cgaaggggtc tccgcccacg ttcacgggca agtggctcct gatcggcggg atcgcctgcg
     2581 tcctcctcgt catcgtgctg ctcgctatcc cacggttccg cgcgttcgcg aagggcaagg
     2641 cctcgga
//
LOCUS       DATDBZ010000123         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1475071, whole genome shotgun sequence.
ACCESSION   DATDBZ010000123 DATDBZ010000000
VERSION     DATDBZ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475071"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VIX62_03040"
     CDS             <1..270
                     /locus_tag="VIX62_03040"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEY5859241.1"
                     /translation="PIVIDGVIADAALLVAERLAPGCAAGCIAGHRSTEPAATIALAA
                     IGLEPLLDLDLRLGEGTGAVLALPLLESAARLLTDVATIAEITGS"
     gene            359..1123
                     /locus_tag="VIX62_03045"
     CDS             359..1123
                     /locus_tag="VIX62_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017237846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY5859242.1"
                     /translation="MAELRFPFSRPQFDSVEQERLHLKQRLAASFRLFGRFGFSEGVA
                     GHITARDPEFPDTFWVNPFAMNFSHIRVSDLIRVDHHGEVVEGEYHVNRAAFAIHSQV
                     HAARPDVIAAAHAHSVYGKAWSSMGRTLDPLTQDSCAFYGDHVLFDDFTGVVLDTEEG
                     KRIAHALGDAKAAILRNHGNLTVGRTVDEATWWFITMERTCQAQLLAEAAGTPVIIDE
                     ESAELTSTQVGSDGAGWLSYQPLYEWITRSEPDLFD"
     gene            complement(1189..1677)
                     /locus_tag="VIX62_03050"
     CDS             complement(1189..1677)
                     /locus_tag="VIX62_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859243.1"
                     /translation="MSTSENDSTPLPSSRIDFVHNSEEQLARLFDFYGIPWEYEPHTY
                     VLSVDERGVTTEAFTPDFWLPDHGAYIEVTTMRQGLVTRKNRKVRRLRELHPDLTIRI
                     VYQRDYLHLLVRFGLEPPSQLEDLDVAATATEAGPSLLTEPPRRRLHPSMPEFRALRR
                     AG"
     gene            complement(1690..>2006)
                     /locus_tag="VIX62_03055"
     CDS             complement(1690..>2006)
                     /locus_tag="VIX62_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5859244.1"
                     /translation="ELKVIKSLLDAGIRLEAVREAFTYMRDHLGEDVTTANLVIRGSS
                     VLVSTDEELVDVVKRGQGVLNVLPLGSVKSEIDAAIVELRPVADTASGDKTSGSRAVG
                     EG"
BASE COUNT          301 a          726 c          647 g          332 t
ORIGIN      
        1 cccatcgtga tcgatggagt catcgccgac gccgcgctcc tggtggccga gcggctcgcc
       61 cccggctgcg ctgccggttg catcgccggc caccgctcga ccgagcccgc cgccacgatc
      121 gctctcgccg ccatcggcct cgagcccctg ctcgatctcg atctccggct tggtgagggc
      181 acaggtgccg tgctggctct ccccctgctc gagtccgcag cgcgtctcct gaccgatgtc
      241 gccaccatcg cggagatcac cggcagctga gcccgatcgg cccaccgaca cgtcgggcga
      301 gccagccggg tggcgcagtt ctacacttgg accagcgctc gcatccggag gtgcacccat
      361 ggccgagctt cggttcccgt tctcccgccc gcagttcgac tccgtcgaac aggagcggct
      421 ccacctcaag cagcggctcg ccgcgtcctt ccgcctgttc gggcgcttcg ggttcagcga
      481 gggcgtcgcc gggcacatca ccgcccggga tcccgagttc cccgacacct tctgggtgaa
      541 cccgttcgcc atgaacttca gccacatccg cgtctcggac ctcatccggg tcgatcacca
      601 cggcgaggtc gtcgagggcg agtaccacgt caacagggcc gcgttcgcca tccactccca
      661 ggtccacgcc gcccgacctg acgtcatcgc cgcggcccac gcccactccg tgtacggcaa
      721 ggcgtggtcg tcgatgggtc gcaccctcga tccgctcacc caggactcgt gcgccttcta
      781 tggcgaccac gtcctgttcg acgacttcac cggcgtcgtg ctcgacaccg aggagggcaa
      841 gcggatcgcc cacgccctcg gcgatgccaa ggccgccatc ctgcgcaacc acggcaacct
      901 caccgtcggc aggacggtcg acgaggccac gtggtggttc atcaccatgg agcgcacctg
      961 tcaggcacag ctgctcgccg aggccgccgg caccccggtg atcatcgacg aggagtcagc
     1021 agagctcacg tcgacccagg tcggcagcga cggtgccgga tggctgagct accagccgct
     1081 ctacgagtgg atcacgcgct ccgaacccga cctgttcgac tgatcgcgcg gcgccaagca
     1141 cagccctgcc cgccgttccg ccagcctgag gttcagccag cctgaggttc agccagcacg
     1201 gcggagcgcc cggaactccg gcatcgacgg gtgcaggcgg cgacgcggcg gttcggtgag
     1261 caggctcggg cctgcctctg tggctgtcgc ggccacgtcc agatcctcga gctgtgacgg
     1321 cggctcgagc ccgaatcgca cgagcaggtg caggtagtcg cgctggtaga cgatccgaat
     1381 cgtcaggtcc gggtgcagtt cccgtagccg gcgcaccttg cggttcttgc gggtgacgag
     1441 gccttggcgc atggtcgtga cctcgatgta ggcgccgtgg tcgggcagcc agaagtcggg
     1501 ggtgaacgcc tcggtggtga cgccgcgctc atcgaccgac agcacatagg tgtgtggctc
     1561 gtactcccac gggatgccgt agaagtcgaa gaggcgggcc agctgctctt cggagttgtg
     1621 gacgaagtcg atccgggacg atggcagtgg tgtgctgtcg ttctccgacg tggacaccgt
     1681 cgccggccct cagccctcgc ccacggcccg ggacccggag gtcttgtcac cggatgcggt
     1741 gtctgcgacg ggccgcagct cgacgatcgc cgcatcgatc tcgctcttga cgctgccgag
     1801 tggaaggacg ttgagcacgc cctggcctcg cttgacgacg tccacgagct cctcgtccgt
     1861 gctgaccagc acgctcgagc cgcggatcac caggttcgcc gtggtgacgt cctccccgag
     1921 gtggtcacgc atgtacgtga acgcctcgcg cacggcctcg agccggatcc ccgcatcgag
     1981 caggctcttg atgaccttga gctcga
//
LOCUS       DATDBZ010000124         2450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2704906, whole genome shotgun sequence.
ACCESSION   DATDBZ010000124 DATDBZ010000000
VERSION     DATDBZ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704906"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VIX62_03060"
     CDS             complement(<1..525)
                     /locus_tag="VIX62_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859245.1"
                     /translation="MIDDREIRDMLQRRADTVPAVPTDTPKAVRRARRRLVMNGALAT
                     VAAIAIVVVAFAGVDAIRSAPLPAIRPTPTPSVPDPTPNDGEEVRGWPDTGRNPPGVY
                     SWDAIGAVSTYGFRCGTSSPLGEGSSCVIGFMHNGRGGGGSVEIHVQQVPAGGITDGG
                     SAVTVAGHEGIYLRI"
     gene            complement(522..1058)
                     /locus_tag="VIX62_03065"
     CDS             complement(522..1058)
                     /locus_tag="VIX62_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5859246.1"
                     /translation="MREGEGGTMSGEMAREGVEDGSLSELYVAYAPDGIRLAFLLTGD
                     RSLAEDLVQDAFVRLVGRLQHLRQPNAFWAYLRRTIVNLATSHFRHGKVERAYLDRLA
                     AAPVADTNDNDELDEAMHRVLLGLPQRQRAAIVLRFYEDLSDVQTAAVLGCSPGTVRS
                     LVSRGMTTLREALEEVPR"
     gene            1279..2142
                     /locus_tag="VIX62_03070"
     CDS             1279..2142
                     /locus_tag="VIX62_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5859247.1"
                     /translation="MSEPAVSTKPDTIVMIHGLWMTPRSWEHWKERYEARGYAVHTPP
                     WPGFEGEVEALNADPSPMEGLAAETVIDSYEEFIQGLSSSPIIMGHSLGGAMTQVLLD
                     RGLGCVGVGVSSATVKGVRDLPFSTLKAAGKVLNPFKRGKPVPMTAKEFHYAFANTMS
                     EEESEPLYERYAVPAASGVLADIALANLHRDPPTEVDFAKEGRIPLLFIGFGEDHVVP
                     PKATEHNERKYDDSVSITEYQEFEGRPHFPGAPGWEEVADYAIDWAERHAGLTVGDHG
                     VARDPARTEGA"
     gene            2183..>2450
                     /locus_tag="VIX62_03075"
     CDS             2183..>2450
                     /locus_tag="VIX62_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859248.1"
                     /translation="MTTHRLQLTRAQILGFRRRVGALDERLPRGRRSLRRAAWAGLQD
                     SMPRAALLSIHARVEGTGSSAWEDPSLVQVWGPRYHVYVVAERDL"
BASE COUNT          415 a          824 c          826 g          385 t
ORIGIN      
        1 gatcctgagg tagatccctt catgcccggc gacggtgacc gcactcccgc cgtcggtgat
       61 gccgcccgcg ggaacctgct ggacgtggat ctcgacggaa ccaccccccc cgcgcccgtt
      121 gtgcatgaaa ccgatgacgc acgaggaccc ttcgccgagc ggcgaggacg ttccgcaacg
      181 gaatccatag gtgctcacag cacctatcgc atcccacgag tacacgcccg gggggttccg
      241 cccggtgtcc ggccagccgc ggacctcctc gccgtcgttc ggtgtcggat cgggcacgct
      301 gggcgtcggc gtcgggcgga tagcggggag cggcgcgctg cggatcgcat cgacacccgc
      361 gaacgccacc acgacgatcg cgatagccgc gaccgtcgcc agggcgccgt tcatgacgag
      421 ccggcgacgg gcccgccgaa ccgccttcgg cgtgtcggta ggcaccgcgg ggaccgtgtc
      481 cgcacggcgt tggagcatgt cgcggatctc tcgatcgtcg atcatcgtgg cacctcttcc
      541 agcgcctcgc ggagcgtcgt catgcctcgc gagacgagtg atcggacggt ccccggtgaa
      601 cacccgagga ctgctgccgt ctggacgtcc gagaggtctt catagaaccg caggacgatc
      661 gccgcgcgct gccgctgggg aagcccgagg aggacgcgat gcatcgcctc gtccagttcg
      721 tcgttgtcgt tcgtgtcagc gacgggagcc gcagccagtc gatcgaggta tgcccgctcg
      781 accttcccgt gccggaagtg gctggtggcg aggttcacga tcgtccgacg caggtaggcc
      841 cagaaggcgt tcggttggcg caggtgctgc aggcgaccga cgagacgcac gaaggcgtcc
      901 tgcacgaggt cctcggccaa ggagcggtcg ccggtcagca ggaaggccag gcggatgccg
      961 tccggcgcgt aggcgacgta gagctccgag agagacccgt cttcaacacc ctccctcgcc
     1021 atctcgcccg acatcgtgcc accttctcct tccctcatgt gaaagagacg cgcaggtgcc
     1081 atccgaacgc tgcgcagggc ctgagatcgg tccgatcctg agcacgtgca gccctcctcg
     1141 gggtcagctc cttcggcagt acgtccatac acccgaacac cgaaccggtc gacttgcacg
     1201 ttggcgtgcg acgatgcacc ttctcgcatt gaacggttca ccagaggtgg cgagcccacc
     1261 cgtcggaagg ggaggacgat gtcagagccc gccgtgagca ccaagcccga cacgatcgtc
     1321 atgatccacg gcctgtggat gacaccgcgg agctgggagc actggaagga acgctacgag
     1381 gctcgtggct atgcggtaca cacgccaccg tggcccggat tcgaggggga ggtcgaggcg
     1441 ctgaatgccg acccctcccc gatggaaggt ctggccgccg agacggtcat cgactcctac
     1501 gaggagttca tccagggtct atcgagttcg ccgatcatca tgggtcactc gctcggcggc
     1561 gcgatgacgc aggtgctgtt ggaccgcggc ctcggctgcg taggggtcgg cgtgtcgtcg
     1621 gcgacggtca agggcgtgcg tgacttgccg ttctcgacat tgaaggccgc cggcaaggtg
     1681 ctcaacccgt tcaagcgggg caagccggtc ccgatgacgg cgaaggagtt ccactatgct
     1741 ttcgcgaaca ccatgagcga ggaggaatcc gagccgctct acgagcggta cgccgtgccg
     1801 gcggcatctg gggtgctcgc cgacatcgcg ctcgcgaacc tccaccgcga tccgcctacc
     1861 gaggtcgact tcgccaagga aggccggatc ccgttgctgt tcatcggctt cggcgaggat
     1921 cacgtcgtac cccccaaggc caccgagcac aacgagcgga agtacgacga ctcggtctcg
     1981 atcacggagt accaggagtt cgaaggccgt ccgcatttcc cgggggctcc gggctgggag
     2041 gaggtcgccg actacgcgat cgactgggca gaacgccatg ccggcctgac ggtgggcgac
     2101 cacggcgtcg ctcgtgatcc cgctcggacg gagggagcct gaactcggat cggccactac
     2161 gcgcccgtcc cctgcagctt cgatgacgac gcatcggctc caactcacgc gggcacagat
     2221 cctgggcttc cggcgacgcg tcggcgcgct cgacgagcgc ctgccccgag ggcggcgctc
     2281 gctccggcga gcggcatggg cgggcctgca ggacagcatg cctcgggcag ccctcctgtc
     2341 gatccatgca cgggtcgaag gaaccggatc atctgcgtgg gaggatccat ccctggtaca
     2401 ggtctggggt ccccgctacc acgtgtacgt cgtggccgaa cgcgacctgc
//
LOCUS       DATDBZ010000125        11027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1269416, whole genome shotgun sequence.
ACCESSION   DATDBZ010000125 DATDBZ010000000
VERSION     DATDBZ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11027
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269416"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VIX62_03080"
     CDS             complement(<1..267)
                     /locus_tag="VIX62_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859249.1"
                     /translation="MPIGSRLTTLLVVLALVALPAVALRAFCVGKSCDSGEATASAAV
                     PFCPLPAELRGLISAGFRQGRSPDVMAATDANGPGVRVDDGPGIA"
     gene            326..1993
                     /locus_tag="VIX62_03085"
     CDS             326..1993
                     /locus_tag="VIX62_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5859250.1"
                     /translation="MASHTYRRRVLIPVLLLLLSAGALSTRGSVPAKAQSSNQDIVRL
                     ACSIPHEQLLRTYRGWRPDRGAQISFIPKEPNFVGSGLPHVGPWDYIQTVPMFWYGPG
                     YIKPLGEVKRPVTSADVAPTAAELLKFDGFHAPDGTPMTEALLPADQRKTPKLVVTMV
                     WDAGGMNVLEAHPTAWPYLKSLIPKGSFFTDATVGSSPTSTAQIHATIGTGSFPDHHG
                     IVGHNMLVAGKIIGPWNQGPSFLVEPTLADIYDRAMDNEPVVGLVGTVDIHFGMLGHG
                     GFFTGGDRDIALTRSVVGGTTLTDEGFEWNLPEHDAAYYRLPTYANKVPGFEQDVAAV
                     DQADGKKDGKWRDNDIDELLQGFDTPARTPYQERVVEEVMTREDFGQDDTPDLLYLNF
                     KEIDFVSHVWSMDSPEMEDAVVAQDAALKEFVTFLDKEVGKGEWVLGLTADHASMPDP
                     SVTGGYQISSGPMQVMINEKFDTDGDDLPIVELMQPTQAFLDLNELKQNGYTVDDVAR
                     YMMTFTQAQTAGGGVVPNPGQENDTVMQAAFPSALMPDLPCLPEARE"
     gene            2033..5710
                     /locus_tag="VIX62_03090"
     CDS             2033..5710
                     /locus_tag="VIX62_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859251.1"
                     /translation="MTATDIATEADASAPTRSGRSSVLGSWWVIAGFAALLLLVLGWK
                     FLADPSLSAPTRDPAWYTWRAQVVLEGEPVRVVQEWGPDGLFAGGYRVSVPVAGALLQ
                     QVVGIDRYSFSAFLMIGIPILTGLALGAAFFRSRKDPLVVLTTLLATVALFLTTPYVG
                     YLDNITVLFLLALMIPFVDAARTSWGARTALFLIAIAAAFTHPTTCVIFGVVLMAVFG
                     FHFLTSRFSLGSALKSDAPMLMSVGFGMIAGLACWVIGIWGKPASLAEAALPPPYSAE
                     FFADRLMQWTGSLQPIVILPFIVIAIGSTILLARATREPARNEDQVSIWWLVAFAGAA
                     TVVTGAAIPYYRFMNASAAPMALVGLGAFVAIRWFASDRPASKVATWAGAALIAWVAI
                     AYVLKDSLSENAPVWVFALMGLLGVLVLIRGLAPSAMPRILAAGLAALLVFGSLGWLL
                     FDGVQNRWVSEANQWASQSVRTSLAAVNEVVAEAGTRPNVLIVNFGDRDEQFDTNTGY
                     GWAKTYTNVFRTGLPGEAIERSTTYFGTLDNFLAGEPTTSTQGSEGYDDTSIAHWCET
                     FGGPKETCDPDDKKPDDFQPRFDQFPEDPVVFLVGQYYGGLCNGAEDCSADTQQQLLD
                     EATAQGVEVGPDVYVIEGDGLWTPPANVVADAQAAAAAEAQKFESHPGALDNLPQNLL
                     VVLLLAFLLIVPGWLASNWLGIRSTPDRIALIPGMSVIFLILSGIGVLAVWRGPLSVT
                     KGWTVVAVAVGVGAALRFADVWLRRPLESFGNFFNGLFGVFSNRDFAILMGVQFLVQA
                     GQGVIQGAIGKSIAFGGKEGFDIQNVPSADYLLKVVLALYVPYTLLSPFIGVFIDRFA
                     RRRVVWWTNLITSAIVGVIAIVVLAPLGDGTTEGKAGPTIGLILALLAAQAVVRVILA
                     VKSAAIPDVLSGKDLMQGNALSQAGGALFQIFGIAFALGAGAVLPAWLVVIGGAVVLI
                     IAAVVAKQMHHVETAGHDTTFGKEARNVVHNVIAGIKEIASRPPAALGLLSFQMLRYQ
                     FWGFGLFVFALYAKNLVQGGGDKADTLALALSGLGGLVGGALGLVLAQKWKDRVPPIR
                     LLLASMTLLGAGTLLFGGMVSVLGFAGMLFVGFFSFFLGKISADTITQQAMPDDFRGR
                     AFALFDIAYNVGFIVPAMILSLIWVENSEPRTRIILIVSGVVFLILTGLVALWARSIR
                     DQFAPQDDLVEVDGEPVVPAG"
     gene            complement(5726..6037)
                     /locus_tag="VIX62_03095"
     CDS             complement(5726..6037)
                     /locus_tag="VIX62_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859252.1"
                     /translation="MPEQPTHEPVIEVARTDVEQFLSKFLVRVTEDDSTSSHSVTLSG
                     ADWDRLGTGYRSPEMFVEACFTFLLQHETKEQILASFDVSQISGYFPDFERAIASRPK
                     D"
     gene            6115..7038
                     /locus_tag="VIX62_03100"
     CDS             6115..7038
                     /locus_tag="VIX62_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5859253.1"
                     /translation="MTGAPIVDRRATDRRGELIGSGLCALMAVLFAVVVIFGKQVQAG
                     RLPFVFLAIRFAGQSVLLMLLVMATRRPLIPAKGERLPLAIAGTIGYGSESAFYFTAL
                     NHGSAAAITLLFYTYPVWVMLATMVLDRKAPPRGLFAALGLALAGSAMVVLGGGGADI
                     ETIGIVLALCTSLAYSAYLIATDRTVKTTDPLTAATWLGIGAAVANLTYAVAFSAVVV
                     PAAGSWWRLAGMAVFSAGAFAAMLAGLQRVGAVRNAIIGVMEPLTVAVLAAVFLSEPV
                     TLPVLVGGTLILGGAVIASLIRTTTTAEPNV"
     gene            7085..7819
                     /locus_tag="VIX62_03105"
     CDS             7085..7819
                     /locus_tag="VIX62_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY5859254.1"
                     /translation="MTDDLCSRDAYLITCDATVTAVGEDGVTLDRTVFYARGGGQPGD
                     TGVLRWDGGEASVADTVKSRETGEIAHVLADGVTAPPVGAAVTAEIDWDRRHMLMRTH
                     TALHALSGIIFTDHGARVTGGNMDDQGSARMDFELDSMSQELGQQIEEQLNRRLAEDV
                     PVHVSFLPRADALNDPDLIRTKVSLIPESVDPIRVIDIEGIDKQADGGTHVRSTAEVG
                     RVRVVKTESKGKANKRMRIQLEDAGA"
     gene            complement(7816..9738)
                     /locus_tag="VIX62_03110"
     CDS             complement(7816..9738)
                     /locus_tag="VIX62_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY5859255.1"
                     /translation="MPHRRHIALVFAGALAALALSPPAAHAEDYGATVHAIDNVFGPA
                     VVRVEPGQSVEWTMDGRSDHTVEADDGSWSSGNLVPGAEFSKTFDEPGVYPYFCRYHG
                     DPGVGMAGTVLVGDVSLPGAADGVGPGREPVPTGFADTVRVPKDAPTIQAGVNEAEPG
                     GLVLVAAGVYEESVTVTTPFVTIRGMDRNDTIIDGGFERAIGIHVIEADGVTIENLTS
                     RNNLLNGFQWTSVHGYRGSYLTAVNNGDYGVFAYDSDWGQFDHSYASGSPDSGFYIGQ
                     CNPCHAVITDVLAEHNALGFSGTNASGDLAIVNSEWRFNMAGIVPNTLDSERLAPQQD
                     ALIAGNFVHDNNSTTADSKDLEYPTFGTGILIAGGLNNQVVGNLVENHESYGIAVLPN
                     LDDTLWVTGGNEVRENLVTGSGRADLALGAPAAGGDCYSGNAFHSSAPPAIELLRGCG
                     SFGARMPGGDPAPTVNLGTRFLDALDGEFPHGDWRSQPAPPDQPTMENPAVAPQIPAV
                     AEAAVPEPYRIRRVGQIDGVLITVGKEMTVFGIPLATSWWSLLIGLYGYALPGFLYAA
                     WLTIALWDLIRQESVPISSRARWMAVVILVPFLGPLLYFAFGRSPIPKQLRLMLTVGG
                     AVGVLIVAVVAALLGG"
     gene            9798..>11027
                     /locus_tag="VIX62_03115"
     CDS             9798..>11027
                     /locus_tag="VIX62_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEY5859256.1"
                     /translation="MPPERLSSLDASFLYLERPSMHMHVAGISMLDPRPDGPLSYDDV
                     QHVVEARLHLAPRLRQRVLRVPGDFARPLWVDDDHFDLDFHLRRSAIPSPGGRFQLER
                     AVGRVLSRPLDPAKPLWELYVFEGLAHQRTAVLLKMHHALADGISGMLIASALFDLSP
                     DTGLGTPPKTPWIPEHPPSRQDLVRDAVQDMVLHPLEAIGQAVGAPQRTLADVAQTWA
                     GVRDVIGMGSPPAGPFDTEIGPARRFAIAETPFERLREVKNVLGGTINDVVLSAVAGG
                     LHELLDARGEPTKGRTLRVMVPVSVRSKAEQGDVGNRVAPAFVDVPVGAMSPRTRMRR
                     VRSFTNQLKESTMAVSADSIIGLGAYSPPALHAMAARLVSRGRWFNLVVSNVPAPQTP
                     LYLAGAPLVASYPAMPLG"
BASE COUNT         1647 a         3788 c         3724 g         1868 t
ORIGIN      
        1 ggcgatcccc gggccgtcgt cgacgcgaac gccaggtccg ttcgcgtcgg tcgcggccat
       61 cacatcgggc gatcgcccct gtcggaatcc cgccgagatc agccctcgga gttccgcagg
      121 caatggacag aacggaaccg cggcgctcgc cgtggcctcg cccgagtcac agctcttgcc
      181 gacgcagaac gcgcgcaagg ccaccgcggg aagggccacc agcgcgagca ccaccaggag
      241 cgtcgtgagg cggctgccga tcggcaccgc tccacgctac cgcctgggtt gtcgttccgc
      301 ccggcgtcgt gttccctatc tcgccatggc ctcccacacc tatcgtcggc gtgtcctcat
      361 ccctgtcctg ctcctcctcc tgagcgccgg cgccctctcc acccgggggt cggtccccgc
      421 gaaggcacaa tcgagcaacc aggacatcgt tcggctggcg tgttccatcc cccatgagca
      481 gctgcttcgc acgtaccggg gctggcgtcc tgaccgggga gcgcagatct cgttcatccc
      541 gaaagagccg aacttcgtcg gctcgggact gccgcacgtg ggcccctggg actacatcca
      601 gaccgtgccg atgttttggt acggccccgg ctacatcaag cccctgggcg aggtgaagcg
      661 gccggtcacc tcagccgatg tggctcccac ggccgccgaa ttgttgaagt tcgatggctt
      721 ccacgccccc gacggcacgc ccatgacgga agccctcctg ccggccgatc aacgcaagac
      781 gcccaagctc gtcgtgacca tggtctggga cgccggcggc atgaacgtgc tggaggccca
      841 tcccacggcg tggccctacc tgaaatccct gatccccaag ggctcgttct tcaccgatgc
      901 caccgtggga tcctcgccca cgtcgacggc gcagatccac gccacgatcg gcacggggtc
      961 cttccctgat caccacggca tcgtcgggca caacatgctc gtggccggca agatcatcgg
     1021 tccgtggaac caggggccgt cgttcctggt cgagcccacg ctcgccgaca tctacgaccg
     1081 ggcgatggac aacgagccgg tggtcgggtt ggtcggcacc gtcgacatcc acttcggcat
     1141 gctgggccat ggcgggttct tcaccggggg cgaccgagac atcgcgctca cgcgttcggt
     1201 cgtgggcggc accaccctga ccgacgaagg gttcgagtgg aacctgcccg aacacgacgc
     1261 cgcgtactac cggctcccca cctacgcgaa caaggtgccg ggcttcgagc aggacgtcgc
     1321 ggccgtggac caggccgacg gcaagaagga cggcaagtgg cgcgacaacg acatcgacga
     1381 gctcctccag ggcttcgaca ccccggcgcg cacgccgtac caggagcgtg tggtggagga
     1441 ggtcatgacg cgtgaggact tcggccagga cgacacgccc gacctgctgt acctgaactt
     1501 caaggagatc gacttcgtga gccacgtctg gtccatggac agcccggaga tggaggacgc
     1561 ggtcgtcgcc caggacgccg ccctcaagga attcgtgacc ttcctggaca aggaggtcgg
     1621 caagggcgag tgggtcctgg gactcaccgc ggaccacgcg tcgatgccgg acccgtccgt
     1681 gaccggcggc taccagatct cctcaggccc catgcaggtc atgatcaacg agaagttcga
     1741 caccgacggc gacgacctgc cgatcgtgga gctcatgcag ccgacgcagg ccttcctcga
     1801 cctgaacgaa ctgaagcaga acggctacac cgtcgacgac gtggcccggt acatgatgac
     1861 gttcacccag gcgcagacgg ccggcggagg cgtcgtcccg aatccgggtc aggagaacga
     1921 caccgtgatg caggcggcgt tcccatcggc gttgatgccc gacctcccct gtttgcccga
     1981 agcgcgcgag taggctgcgc tcggagcagg cacgagaagg ggaccccaac cgatgaccgc
     2041 caccgacatc gcgaccgagg ctgacgcgag cgcacccacc cgttccgggc gctccagcgt
     2101 gctcggttcc tggtgggtga tcgcggggtt cgccgcgctg ctgctgctgg tcctcggctg
     2161 gaagttcctg gccgacccca gtctctctgc gcccacgcgc gacccggcct ggtacacgtg
     2221 gcgcgcgcag gtggtgctgg aaggcgagcc cgtccgcgtc gtccaggaat ggggtccgga
     2281 cggcctgttc gcgggcggct accgcgtcag cgttcccgtg gccggcgcgt tgctgcagca
     2341 agtcgttggc atcgatcggt actcgttcag cgcgttcctg atgatcggca tcccgatcct
     2401 cacgggcctc gcgctgggcg ccgccttctt ccgcagccgc aaggacccgt tggtggtgct
     2461 gaccaccctg ttggcgacgg tcgcgctgtt cctcaccacg ccgtacgtcg gctacctcga
     2521 caacatcacc gtgctgttcc tgctggcgtt gatgatcccg ttcgtcgacg ccgcgcgaac
     2581 atcgtggggc gcgagaaccg cgctgttcct gatcgcgatc gcggcggcct tcacccaccc
     2641 caccacgtgc gtgatcttcg gcgtggtgtt gatggcggtg ttcgggttcc atttcctgac
     2701 gagtcggttc tcgctcgggt cggcgctgaa atcggacgcc ccgatgctga tgtccgtcgg
     2761 gttcggcatg atcgccgggc tggcgtgctg ggtgatcggc atctggggca agccggcgag
     2821 cctggccgaa gccgcgctgc caccgccgta ctccgcggag ttcttcgccg accgcctgat
     2881 gcagtggacg ggctccttac agccgatcgt gatcctgccg ttcatcgtga tcgcgatcgg
     2941 ttccacgatc ctgctggcgc gcgccacgcg cgaacccgcg cgcaacgagg accaggtgtc
     3001 gatctggtgg ctcgtcgcct tcgccggcgc cgccaccgtg gtgaccggcg cggcgatccc
     3061 ctactaccgg ttcatgaacg cgtcggccgc ccccatggcg ctggtgggtc tgggggcgtt
     3121 cgtggccatc cgatggttcg cctcggatcg tccggcctcc aaggtcgcga cctgggcggg
     3181 cgcggcgctc atcgcgtggg tggcgatcgc ctacgtgttg aaggactcgt tgtcggagaa
     3241 cgcgcccgtg tgggtgttcg cgctcatggg gttgctcggc gtcctggtcc tgatccgcgg
     3301 cctcgccccg tcggcgatgc cacgcatcct ggcagccggg ctggccgccc tgctggtgtt
     3361 cggctcgctc ggctggctgt tgttcgacgg tgtccagaac cggtgggtca gcgaggcgaa
     3421 ccagtgggcc agccagagcg tgcgcacgtc cctggcggcc gtgaacgagg tcgtcgccga
     3481 ggcgggtacg cgtccgaacg tgctgatcgt gaacttcggc gaccgcgacg aacagttcga
     3541 caccaacacc gggtacgggt gggccaagac ctacacgaac gtcttccgca ccggactgcc
     3601 cggcgaggcg atcgaacgct cgaccaccta cttcggcacg ctcgacaact tcctggcggg
     3661 cgagcccacg acgtcaaccc aggggagcga gggctacgac gacacgtcga tcgcgcactg
     3721 gtgcgagacc ttcggcggcc ccaaggagac gtgcgacccc gacgacaaga agcccgatga
     3781 cttccagccg cggttcgacc agttccccga ggatccggtg gtgttcctgg tcggccagta
     3841 ctacggcggc ttgtgcaacg gggccgagga ctgctcggcc gacacccagc agcagctcct
     3901 cgatgaggcc acggcgcagg gcgtcgaggt cggccccgac gtctacgtca tcgagggcga
     3961 cggcctgtgg accccgcccg cgaacgtcgt tgccgacgcg caagcagccg cggccgccga
     4021 agcgcagaag ttcgaatcgc accccggcgc gttggacaac ctcccgcaga acctgctcgt
     4081 ggtcctgctg ctcgcgttct tgctgatcgt gcccgggtgg ctcgcgtcga actggctcgg
     4141 catccggtcg acgcccgacc gcatcgcgct catcccgggc atgtccgtga tcttcctgat
     4201 cctgtccggc atcggagtgt tggcggtgtg gcgcggcccg ctctcggtga cgaagggctg
     4261 gaccgtggtc gcggtggccg tcggtgtggg cgcggcgctg cggttcgccg atgtgtggct
     4321 ccgtcgcccg ctcgagtcgt tcggcaactt cttcaacggc ctgttcgggg tcttctccaa
     4381 ccgcgacttc gcgatcctga tgggcgtgca gttcctggtg caggccggcc agggtgtgat
     4441 ccagggcgcg atcggcaagt cgatcgcgtt cggcggcaag gaaggcttcg acatccagaa
     4501 cgtgccgtcg gcggactacc tgctgaaggt ggtgctggcg ctgtacgtgc cgtacacgct
     4561 gctctcgccg ttcatcggcg tgttcatcga ccggttcgca cgtcgccgtg tcgtgtggtg
     4621 gaccaacctc atcacgtcgg cgatcgtcgg cgtgatcgcc atcgtcgtgc tcgcgcccct
     4681 cggcgacggc acgaccgaag gcaaggccgg tccgacgatc ggcctgatcc tggccctcct
     4741 cgcggctcag gccgtggtcc gcgtgatcct ggccgtgaag tcggcggcga tccccgacgt
     4801 gctgtcgggc aaggatctga tgcagggcaa cgccctctcg caagccggtg gcgcgctctt
     4861 ccagatcttc ggcatcgcgt tcgcgctcgg cgccggcgcc gtgctgccgg catggctggt
     4921 cgtgatcggt ggcgcggtgg tgctgatcat cgccgcggtc gtcgcgaagc agatgcatca
     4981 cgtcgagacc gcgggccacg acaccacgtt cggcaaggaa gctcgcaacg tggtgcacaa
     5041 cgtgatcgcg gggatcaagg agatcgcgtc gcggccgccg gcggcgctcg gcctgctgtc
     5101 gttccagatg ttgcgttatc agttctgggg cttcggcctg ttcgtgttcg cgctctacgc
     5161 caagaacctg gtgcaaggcg gcggcgacaa ggccgacacg ctggcactgg cgctctcggg
     5221 cctcgggggc ctggtcggcg gtgcgctcgg tctcgtgctg gcgcagaagt ggaaggaccg
     5281 tgttccgccg atccgtctgc tgctcgcatc catgacgctc ctgggcgccg gcacgctgct
     5341 gttcggcggc atggtgtcgg tccttggctt cgccgggatg ctgttcgtcg ggttcttctc
     5401 gttcttcctg ggcaagatct cggcggacac gatcacgcag caggcgatgc ccgatgactt
     5461 ccgagggcgc gcgttcgcgc tgttcgacat cgcctacaac gtgggcttca tcgtgcccgc
     5521 catgatcctg tcgctcatct gggtggagaa cagcgagccg cgcaccagga tcatcctgat
     5581 cgtgtccggt gtggtgttcc tgatcctgac gggactggtg gccttgtggg ctcggtcgat
     5641 ccgcgatcag ttcgcgcctc aggacgacct cgtcgaggtc gacggcgaac ccgtcgttcc
     5701 cgcgggctag tccgcgagcg cgctgtcagt ccttcgggcg tgaagcgatc gcgcgctcga
     5761 agtctggaaa gtagccggag atctggctca cgtcgaacga cgccaggatc tgttccttgg
     5821 tctcgtgctg gagcaggaac gtgaagcagg cctcgacgaa catctcaggt gagcgatagc
     5881 cggtgcccag ccggtcccaa tcggcgccgg acagcgtgac cgaatggctc gaggtgctgt
     5941 cgtcctcggt gacccgcacc aggaacttcg acaggaactg ctcgacgtcc gtgcgggcga
     6001 cctcgatcac gggttcgtgc gtgggctgtt cgggcatctc cgccggaatc gtacgctctg
     6061 ttcccgctcg aacccaagga cctccatggc gcaacgcatg aacaggatgc agcggtgacc
     6121 ggggccccga tcgtcgaccg tcgagcgacc gaccgccggg gtgagttgat cggctcaggg
     6181 ctctgcgcgc tgatggccgt cctgttcgcg gtggtggtga tcttcggcaa gcaggtgcag
     6241 gccggccgcc tgccgttcgt gttcctcgcg atccggttcg cgggtcagtc ggtgctgctg
     6301 atgctgctgg tgatggcgac ccgccgcccc ttgatccccg ccaagggaga acgcctgccg
     6361 ctcgcgatcg ccggcacgat cggctacggc tcggagtcgg cgttctactt caccgcgctg
     6421 aaccatggga gcgccgccgc gatcaccttg ctcttctaca cctatccggt gtgggtgatg
     6481 ctcgcgacga tggtcctgga ccggaaggcg ccgccccggg ggttgttcgc ggcgctcggc
     6541 ctcgcgctcg cgggcagcgc catggtggtg ctggggggcg gcggtgccga catcgagacg
     6601 atcgggatcg tgctggcgct ctgcacgtca ctggcctact ccgcgtatct catcgcgacc
     6661 gaccgaacgg tgaagacgac cgatccgctc acggcggcca cgtggctcgg catcggcgcc
     6721 gccgtggcga acctcacgta cgccgtggcc ttcagcgcgg tcgtcgtgcc ggcggcgggg
     6781 tcgtggtggc gtctcgccgg catggcggtg ttctccgcgg gagcgttcgc ggcgatgctg
     6841 gcggggctcc agcgcgtcgg cgcggtgcgc aacgccatca tcggcgtcat ggagccgctg
     6901 acggtggccg tgctcgcggc cgtgttcctc agcgaacccg tcacgctgcc ggtgctcgtc
     6961 ggcggcactt tgatcctggg cggcgccgtg atcgcgagcc tgatccgcac gaccacgacc
     7021 gccgagccga acgtctagcg tcttggctcg ccacccgccc ggatacttgc ggtagcgtcg
     7081 aaccatgacc gacgacctgt gctcacgcga cgcctacctc atcacctgtg atgccaccgt
     7141 caccgccgtg ggcgaggacg gtgtcaccct ggaccgcacc gtgttctacg cccggggtgg
     7201 tggtcagccc ggcgataccg gggtgctccg atgggacggt ggtgaagcgt ccgtggccga
     7261 taccgtcaag tcgagggaga ccggcgagat cgcccacgtg ctcgccgacg gcgtgaccgc
     7321 gcctccggtg ggagcggccg tgaccgccga gatcgattgg gatcgccggc acatgttgat
     7381 gcgcacccat acggcgctcc acgcgctgag cggcatcatc ttcaccgatc acggcgcacg
     7441 ggtcaccggc ggcaacatgg acgaccaggg ttcggcgcgc atggacttcg aactcgacag
     7501 catgagccag gaactcggcc agcagatcga ggagcagctc aaccggcggc tcgccgagga
     7561 cgtgccggtg cacgtcagct tcctgccgcg cgccgacgcc ctcaacgacc ccgacctgat
     7621 ccgcacgaag gtttcgctga tcccggagtc ggtcgacccg atccgcgtga tcgatatcga
     7681 gggcatcgac aagcaggctg acggcggcac ccatgtgcgt tcgaccgccg aggtcggccg
     7741 cgtgcgagtg gtgaagaccg agtccaaggg caaagccaac aagcgcatgc gcatccaact
     7801 cgaggacgcc ggcgcctagc cgccgaggag cgctgcaacc accgcgacga tcagcacgcc
     7861 caccgcgccg cccacggtca gcatcaggcg gagctgcttc gggatcggag atcgcccgaa
     7921 cgcgaaatag aggagtggcc cgaggaacgg cacgaggatc acgacggcca tccatcgggc
     7981 gcgcgagctg atcggcacgg attcctggcg gatcagatcc cagagcgcga tcgtgagcca
     8041 tgccgcatac aggaacccgg ggagcgcgta gccgtagagg ccgatcaaca ggctccacca
     8101 cgacgtggcg agcgggatcc cgaacacggt catctccttc ccgacggtga tcagcacacc
     8161 gtcgatctgt cctacgcggc ggatacggta cggctccggg accgccgcct ccgccaccgc
     8221 ggggatctgg ggcgcgaccg cggggttctc catcgtgggt tggtccggtg gggcgggttg
     8281 ggagcgccag tccccgtgcg ggaactcgcc gtccaaggcg tccaggaagc gggtccccag
     8341 gttcaccgtg ggcgcggggt cgccgccggg catgcgtgcc ccgaacgagc cgcatccccg
     8401 cagcaactcg atcgcgggcg gcgcgctgct gtggaacgcg ttcccgctgt aacagtcacc
     8461 gccggcggcc ggcgcgccca acgccaggtc ggcccggccc gagcccgtca ccaggttctc
     8521 gcgcacttcg ttgccgccgg tcacccacag ggtgtcgtcg aggttcggga gcacagcgat
     8581 cccgtagctc tcgtggttct ccacgagatt gcccaccacc tggttgttca ggccgcccgc
     8641 gatgaggatc ccggtcccga acgtcgggta ctccagatcc ttcgaatccg cggtggtcga
     8701 gttgttgtcg tgcacgaagt tcccggcgat cagcgcatcc tgctgcgggg cgagccgctc
     8761 ggagtccagc gtgttgggca cgatgccggc catgttgaac cgccactcgc tgttcacgat
     8821 cgcgaggtcg ccgctggcgt tcgtcccgct gaacccgagt gcgttgtgct cggccagcac
     8881 gtcggtgatc accgcatggc acgggttgca ctgcccgatg tagaaccccg aatccggcga
     8941 cccgctcgcg tacgaatgat cgaactgccc ccaatccgag tcgtaggcga agacgccgta
     9001 gtccccgttg ttgaccgcgg tgaggtagga gccgcggtag ccgtgcacgc tcgtccattg
     9061 gaacccgttc aacaggttgt tgcgtgacgt gaggttctcg atcgtcacgc catcggcctc
     9121 gatcacgtgg atgccgatcg cccgttcgaa cccgccgtcg atgatcgtgt cgttgcgatc
     9181 catgccgcgg atcgtcacga agggtgtcgt cacggtcacc gattcctcgt agacgcccgc
     9241 ggcgaccagt acgagccctc ccggctccgc ctcgttcacg cccgcctgga tcgtcggtgc
     9301 gtccttgggc actcgtacgg tgtcggcgaa cccggtcggt accggctcgc ggccggggcc
     9361 caccccatcg gcggcgccgg gcagcgatac gtcgcccacc aggaccgtgc cggccatccc
     9421 cactcccgga tcgccgtgat agcggcagaa gtacgggtag acgcccggct catcgaacgt
     9481 cttcgagaac tcggcgccgg gcacgaggtt gcccgaggac caggaaccgt cgtcggcctc
     9541 caccgtgtgg tcgctgcgcc cgtccatcgt ccactcgacg gactggccgg gttcgacgcg
     9601 gacgaccgcg ggtccgaaga cgttgtcgat cgcgtggacg gtggcgccgt agtcctcggc
     9661 gtgggcggcc ggtggcgaca aggccaaagc cgccagcgcc ccggcgaaca cgagggcgat
     9721 gtggcgacgg tgtggcatcg cgccgcaccc tagcaccccc tcgcctccgg caagcccgcg
     9781 gcggtacgct cgatgacatg ccgcccgaac gcctctccag cctcgacgcc agcttcctgt
     9841 acctggagcg tccgtcgatg cacatgcacg tcgcgggcat ctccatgctc gaccctcgcc
     9901 cggacggtcc gctgtcctac gacgacgtgc agcacgtcgt ggaagcgcga ctgcacctcg
     9961 cgccccgcct gcgacagcgg gtgctccgtg tgccgggtga tttcgcgcgg ccgctgtggg
    10021 tcgacgatga tcatttcgac ctcgatttcc acctgcgtcg gtcggcgatc ccgtcgccgg
    10081 ggggccgctt ccagttggaa cgagcggtgg gacgtgtgct gtcacgtccc ctcgacccgg
    10141 ccaagcccct gtgggagctc tacgtgttcg aaggcctcgc gcaccagcgc acggccgtgc
    10201 tgctgaagat gcaccatgcg ctcgccgacg gcatctcggg catgctgatc gcttcggccc
    10261 tgttcgacct gtcacccgac accggcttgg gcaccccgcc gaagacgccg tggatccccg
    10321 agcaccctcc cagtcggcag gacctggttc gcgacgcggt acaggacatg gtattgcacc
    10381 cgctcgaagc gatcgggcaa gcggtgggcg caccccagcg gaccctcgcc gatgtcgccc
    10441 agacctgggc gggtgttcgc gacgtgatcg gcatgggctc gccgcccgcc ggacccttcg
    10501 acaccgagat cgggcccgct cgccggttcg cgatcgccga gaccccgttc gaacggctcc
    10561 gagaggtcaa gaacgtgctg ggcggcacga tcaacgacgt cgtgctcagc gctgtcgcgg
    10621 ggggtctgca cgaactcctc gatgcacgcg gtgaacccac caagggccga acgcttcgcg
    10681 tgatggtgcc ggtgtcggtg cggtccaagg ccgagcaagg cgatgtcggc aaccgggtgg
    10741 ctcccgcgtt cgtcgacgtg ccggtgggcg cgatgagccc ccggacgcgc atgcggcggg
    10801 tccgctcgtt cacgaaccag ctcaaggaat cgacgatggc ggtgagcgcc gactcgatca
    10861 tcggactggg cgcgtactca ccgcccgcgc tgcatgcgat ggcggcgagg ctcgtgtccc
    10921 gtggccgatg gttcaacctg gtggtgtcca acgtcccggc gccacagacc ccgctgtacc
    10981 tggcgggcgc accgttggtg gccagctacc ccgcgatgcc gctggga
//
LOCUS       DATDBZ010000126         5744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_250913, whole genome shotgun sequence.
ACCESSION   DATDBZ010000126 DATDBZ010000000
VERSION     DATDBZ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250913"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1252
                     /locus_tag="VIX62_03120"
     CDS             68..1252
                     /locus_tag="VIX62_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY5859257.1"
                     /translation="MSADIYLDGASATPLLPEAREAFVAALDTYGDPLNIHASGRAAR
                     SLLDEGRAIVAEAIGAQPDEVVFTSGGTESVALAIWGGVRAMRELGTRVVVGAVEHPA
                     VGGVGNALQSDGFEMIEIPADEYGRIDLDRYVAEIRVPGTLLASVQHANHELGTMQQI
                     GEAARLAREAKVRFHTDACQTVGRLPVDVATLGVDLLSLSAHKFGGPPGVGALYVRRG
                     VGVTAYPCGDDRERKRRSGMENTPGIASMAAALTASLATMGDVAAAQWSLTAHLRERI
                     EATVPGARVHGHPTHRTPHLVCFSVDGLDPAVLMMALDDRGFRLAAGSLCSGRPEDAS
                     PVLERIGLPGTSAFRIGLGRASTREDIDALLAILPDVVEELRQVEAVSTEALARFRPP
                     SG"
     gene            complement(1254..2291)
                     /locus_tag="VIX62_03125"
     CDS             complement(1254..2291)
                     /locus_tag="VIX62_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY5859258.1"
                     /translation="MGDQTIDDGNGGVATLNKKDFTSDQEVRWCPGCGDYAILSAVQG
                     FMPELGIEPHKTVFVSGIGCSGRFTYYMDTYGVHGIHGRAPAIATGIATANPDLSVWV
                     VTGDGDGLSIGGNHLIHALRRNVNIKILLFNNQIYGLTKGQYSPTSEEGKVTKSTPFG
                     SVDHPFNPIAVALGAEASFVARTIDNDRQHLTEVLRQAAAHRGTAFVEIYQNCNVFND
                     GAFDLLREKSQGVHHQIRLVDGEPIIFDEGNKCVIVGDNGRLQVKPTPEVDPDRIVVH
                     QAGGRPSLPFGLAHLSLGPHEATCLGVFRNFDRPVYGEAMHAQLQSVEERMGKGDLGK
                     LLHGGDTWTVS"
     gene            complement(2284..4116)
                     /locus_tag="VIX62_03130"
     CDS             complement(2284..4116)
                     /locus_tag="VIX62_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY5859259.1"
                     /translation="MSKAVLEKERIVVRFAGDSGDGMQLTGDRFTSATAVLGNDLATL
                     PDFPAEIRAPAGTVHGVSAFQIQFASTDITTPGDHADVLIAMNPAALKADLHTVERGG
                     TVILNEDAFTTRNIEKAGYAADPRQDGTLEGFQLFEVPMTSITVRATEEIGISKKEAE
                     RAKNMFALGLVSWMFGRPTETTLNWLERKFGSKQEIFEANVAAFKAGYNFGETTELFA
                     QSYQVAAAPVEAGTYRNVAGSTALSWGLVAAAERSGLTLFYASYPITPASELLHELSR
                     HKNFGVITLQAEDEIAAANMALGAAFAGDLAVTGTSGPGMDLKAETLGLAVIMELPMV
                     IVDVQRGGPSTGLPTKTEQADLLLAMFGRHGESPMPIVAASSPSDCFAVAMEAARIAV
                     RYRTPVIVLSDTFLSNSSEPWKVPEVDDLPLIEPNFATANGEFLPYVRDEQLARPWAK
                     PGTEGLVHRVGGLEREDGTGNISYDPENHERMTHLRQAKVDGIAADIPALEVDDPDGD
                     AELLILGWGSSLGTIRASSRRVRLAGRKVATAHLRHLNPFPANTGDVVKAYPKVLIPE
                     MNMGQLSMLIRARFLVDAISFTKVQGLPIFADDLEQEILRVLDG"
     gene            4220..5170
                     /locus_tag="VIX62_03135"
     CDS             4220..5170
                     /locus_tag="VIX62_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5859260.1"
                     /translation="MQTNQLGSNGPDLSVIGYGAWEAGGTNWGPNESDDAVIAAMRAA
                     IDAGINWIDTAEVYGKGVSESLVGRAVAGRRDEVVIASKVAPQPEGSGFRPEQVHRAC
                     EASLGRLDIDVIDLYQLHWPDDQDTPIEDTWGAMVELQEAGRVRWIGVSNFSQDLIER
                     CLAIHHVDSLQPEFSTLNRRNAELIRWCGEQGVGVVSYGPLAFGLLTGAITQDTVFPE
                     GDWRGSEDEEDDPDEDDVDLFDPDTLPQALATVERLRPVADRLGITLSQLALAWNVNQ
                     AGVTSAIAGSRNPDHVRANAEAGDVKLDDATLAEVDALLS"
     gene            complement(5180..5677)
                     /locus_tag="VIX62_03140"
     CDS             complement(5180..5677)
                     /locus_tag="VIX62_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5859261.1"
                     /translation="MSQSLPDMVRRGSGSQVGDRRPEQAPRRTKRQTATALAGVPMFA
                     GFSKRHLHRLAADTDVLAFGTGESIVREGDPGETLFVVLSGQGRVVRGKKRVGSVVPG
                     DFFGELSAIDGGPRTASVIAETPVQVLRLFRRTLMALIKDEPQVSLKLLDGITRRVRQ
                     VDRLQ"
BASE COUNT          866 a         1984 c         1971 g          923 t
ORIGIN      
        1 cgacgggccg gcggaccact ccgccggccg tcgtgcgttc accgatctcg acgctaggct
       61 ctgcaggatg agcgccgaca tctacctcga cggggcgagc gccaccccgc tgctccccga
      121 agctcgcgag gcgttcgtcg cagccctcga cacgtacggc gatccgctca acatccacgc
      181 ctcgggccgc gctgcgcgtt cgctcctgga cgagggacgc gccatcgtgg ccgaggcgat
      241 cggtgcgcag cccgacgagg tcgtcttcac ctccggtggc accgagtcgg tggcactggc
      301 gatctggggt ggtgtccgag ccatgcgcga gctcggcacg cgagtggtcg tgggcgcggt
      361 ggagcatccg gccgtgggcg gggtcggcaa cgcgctgcag agcgacgggt tcgagatgat
      421 cgagatcccc gccgacgagt acggtcggat cgacctggac cgctacgtcg ccgagatccg
      481 cgttcccggg acgctgctcg ccagcgtgca acacgccaac cacgagctgg ggaccatgca
      541 gcagatcggc gaggccgctc gcctggcccg cgaggcgaag gtccggttcc acaccgacgc
      601 ctgccagacc gtcgggcgac ttcccgtcga cgtcgcgaca ctgggcgtcg acctgctgtc
      661 gctgtcggcc cacaagttcg gcggcccgcc cggcgtgggc gcgctgtacg tgcgacgcgg
      721 cgtcggggtg accgcctatc cgtgcggcga cgatcgcgag cgcaagcgcc gctcgggcat
      781 ggagaacacc cccgggatcg cctcgatggc ggcggcgctc accgcatccc tggccaccat
      841 gggcgatgtc gcggccgcgc agtggtcgct caccgcccac ctgcgcgaac gcatcgaggc
      901 caccgtgccg ggcgcccgcg tgcacggcca tcccacgcac cggaccccgc acctcgtgtg
      961 cttcagcgtg gacggactgg accccgcggt cctcatgatg gcgttggacg accgcgggtt
     1021 ccgcttggcc gccgggtccc tctgcagcgg ccgtcccgag gacgcctcac ctgtgctgga
     1081 gcggatcggc ctgccgggca cgagcgcgtt ccgcatcggc ctcggcaggg cgagcacccg
     1141 agaggacatc gacgcgctgt tggcgatcct tccggacgtg gtcgaagaac tgcgtcaggt
     1201 cgaagcggtc agcaccgaag cgctcgcccg gttccgacct ccgagcggtt gagctagctc
     1261 acggtccagg tgtcgccgcc gtgcagcagc ttgccgaggt cgcccttgcc catgcgttcc
     1321 tcgacgctct gcagctgcgc gtgcatcgcc tcgccgtaca ccggccgatc gaagttgcgg
     1381 aacacgccga ggcaggtcgc ctcatgcggc cccagcgaca ggtgggcgag tccgaacggc
     1441 aatgaggggc gaccgcccgc ctggtgcacc acgatgcggt cgggatcgac ctcgggggtg
     1501 ggcttgacct ggagccgacc gttgtcgccg acgatcacgc acttgttgcc ctcgtcgaag
     1561 atgatcggct cgccgtcgac caatcggatc tggtggtgca cgccctgcga cttctcgcgc
     1621 agcaggtcga acgcgccgtc gttgaacacg ttgcagttct gatagatctc gacgaacgcg
     1681 gtcccccgat gcgcggcggc ctgccgcaac acctccgtga ggtgctgacg gtcgttgtcg
     1741 atcgtgcgcg ccacgaacga cgcctcggcg cccagggcaa cggcgatcgg gttgaacggg
     1801 tggtccaccg agccgaacgg cgtcgacttc gtgaccttcc cttcctcgct cgtcggcgag
     1861 tactggccct tcgtgaggcc gtagatctgg ttgttgaaca gcaggatctt gatgttcacg
     1921 tttcgccgga gcgcgtggat caggtggttg ccgccgatcg agagcccgtc accgtctccg
     1981 gtgaccaccc acaccgacag gtcggggttc gccgtggcga tgccggtggc gatcgccggc
     2041 gcgcgcccgt ggatcccgtg caccccgtag gtgtccatgt agtaggtgaa ccggccgctg
     2101 cagccgatgc cgctcacgaa cacggtcttg tgcggctcga tgccgagttc gggcatgaag
     2161 ccctggaccg cggacaagat cgcgtagtcg ccgcagcccg gacaccagcg cacctcctgg
     2221 tcggaggtga agtccttctt gttcagcgtg gcgacgccgc cgttgccgtc gtcgatcgtt
     2281 tgatcaccca tcgagcaccc gcaggatctc ctgctccagg tcgtccgcga agatcgggag
     2341 cccctggacc ttggtgaagc tgatggcatc caccaggaac ctcgcccgga tcagcatgga
     2401 cagctggccc atgttcatct cggggatcag caccttggga taggccttga ccacgtcgcc
     2461 ggtgttggcc gggaacggat tgaggtgacg cagatgcgcc gtggcgacct tgcgcccggc
     2521 caaccgcacc cgtcgcgacg aggcccggat cgtgcccagc gacgaacccc atcccaggat
     2581 caggagctcg gcgtcgccgt cggggtcgtc gacctcgagc gccgggatgt cggccgcgat
     2641 gccgtcgacc ttggcctggc gcaggtgcgt catgcgctcg tggttctccg ggtcgtagct
     2701 gatgttgccg gtgccgtcct cgcgctcgag gccaccgacc cggtgcacga gtccctcggt
     2761 gccgggcttg gcccaggggc gcgccagttg ctcgtcgcgc acgtacggca ggaactcacc
     2821 gttggccgtg gcgaagttcg gctcgatcag cggcaggtcg tcgacctcgg gcaccttcca
     2881 cggttccgag gagttcgaca ggaacgtgtc ggacagcacg atcaccgggg tgcggtagcg
     2941 caccgcgatc cgcgcagcct ccatggcgac ggcgaagcag tccgacggcg aggacgccgc
     3001 cacgatgggc atgggtgact cgccgtggcg gccgaacatc gcgagcagca ggtcagcctg
     3061 ttcggtcttg gtgggcaggc ccgtggacgg gccgccgcgc tggacgtcca cgatgaccat
     3121 gggcagttcc atgatcacgg ccaggcccag cgtctcggcc ttgaggtcca tgccggggcc
     3181 actggtgccg gtcaccgcga ggtcgccggc gaacgccgcg cccagcgcca tgttcgcggc
     3241 cgcgatctcg tcctcggcct gcaacgtgat gacgccgaag ttcttgtgcc ggctgagctc
     3301 gtgcagcagc tccgaggccg gggtgatcgg gtagctggcg tagaacaacg tgagtccgct
     3361 gcgctcggcg gccgccacca gaccccagga cagcgccgtc gaccccgcga cgttgcgata
     3421 ggtcccggct tcgaccgggg cggcagccac ttggtaggac tgcgcgaaca gctccgtggt
     3481 ctcgccgaag ttgtagccgg ccttgaacgc cgcgacgttc gcctcgaaga tctcctgctt
     3541 gctgccgaac ttgcgctcga gccagttcag cgtggtttcg gtggggcgcc cgaacatcca
     3601 cgagaccagg cccagcgcga acatgttctt ggcgcgttcg gcttccttct tgctgatgcc
     3661 gatctcctcg gtggcccgca ccgtgatcga ggtcatgggc acctcgaaca gctggaagcc
     3721 ttcgagggtg ccgtcctgcc gcggatcggc ggcatagccc gccttctcga tgttgcgcgt
     3781 ggtgaaggcg tcctcgttga ggatcaccgt gccaccgcgc tccacggtgt gcaggtccgc
     3841 cttgagcgca gcggggttca tggcgatcag cacatcggcg tggtcgcccg gcgtggtgat
     3901 gtcggtggag gcgaactgga tctggaaggc cgagaccccg tggacggtgc cggccggggc
     3961 gcggatctcc gccgggaagt ccggaagggt cgcgaggtcg ttgccgagca cggccgtggc
     4021 cgacgtgaac cgatcgccgg tgagctgcat cccatcgccg gagtccccgg cgaagcggac
     4081 gacgatccgt tccttctcga gaacggcctt gctcatcgtg gggacctgct caacagctgg
     4141 agactcctcg gtcgggggtt actcacctag cgtactgcgc tgggcagcga gcgccgcgca
     4201 ccagcggtag attctcccca tgcagacgaa tcagctcgga tcgaacggcc ccgacctgtc
     4261 ggtgatcggc tacggcgcgt gggaggccgg cggcaccaac tggggtccca acgagtccga
     4321 cgatgccgtg atcgccgcca tgcgcgccgc gatcgacgcc ggcatcaact ggatcgacac
     4381 ggccgaggtg tacggcaaag gggtctcgga gtcgctggtg ggacgggccg tcgccggacg
     4441 ccgcgacgag gtggtgatcg cctcgaaggt cgccccgcag ccagaaggct ccggcttccg
     4501 cccagaacag gtccatcggg cctgcgaggc atcgttgggc cgcctcgaca tcgacgtgat
     4561 cgacctctat cagctgcact ggcccgacga ccaggacacc ccgatcgagg acacctgggg
     4621 cgccatggtg gaactgcagg aagcggggcg cgtccggtgg atcggcgtct cgaacttctc
     4681 ccaggacctc atcgagcggt gcctcgcgat ccatcacgtg gactcgctgc agccggagtt
     4741 ctccacgctg aatcgccgca acgcggagct gatccgctgg tgcggtgagc agggggtagg
     4801 cgtcgtctcc tacgggccac tggcgttcgg gctgctcacc ggggccatca cccaggacac
     4861 cgtgttcccc gaaggcgact ggcgaggcag cgaggacgaa gaggacgatc ccgacgagga
     4921 cgacgtcgac ctgttcgacc ccgacaccct gccgcaagcg ctggccaccg tcgaacgcct
     4981 gcgtccggtc gccgaccggc tgggcatcac cctctcgcag ctggccctgg cctggaacgt
     5041 gaaccaggcc ggcgtcacca gcgcgatcgc gggcagccgt aaccccgatc acgtgcgggc
     5101 gaacgccgag gccggcgacg tgaagctcga cgacgctacg ctggcagagg tcgatgctct
     5161 gctgagctag gtgccaggtt cactgcaggc gatcgacctg gcgcacccgc cgggtgatgc
     5221 cgtccagcag cttcaggctc acctggggct cgtccttgat cagcgccatg agcgtccggc
     5281 ggaacaaccg gagcacctgc accggcgttt cggcgatcac cgaggccgtc ctcggcccac
     5341 cgtcgatcgc ggaaagttcc ccgaagaagt cgcccggcac caccgatccg acccgtttct
     5401 tgccgcgcac gaccctgccc tgaccggaca ggacgacgaa gagcgtctcc cccgggtcgc
     5461 cttcgcgaac gatcgattcg cccgtcccga acgcgaggac gtcggtgtcg gcagcgagac
     5521 ggtgcaggtg gcgcttcgag aagccggcga acatcggcac gcccgccagc gctgtcgcgg
     5581 tctgccgttt ggttcgtcga ggcgcctgct cgggtcgccg gtcacccacc tgactacctg
     5641 atccgcgacg caccatgtcc ggcaggctct ggctcatggc gcgagcctac cccgccgcgg
     5701 ctttcgggcg ccaacctcgg gtgtcttcgc tggtcgcacc cttc
//
LOCUS       DATDBZ010000127         6381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1269667, whole genome shotgun sequence.
ACCESSION   DATDBZ010000127 DATDBZ010000000
VERSION     DATDBZ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269667"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VIX62_03145"
     CDS             complement(<1..215)
                     /locus_tag="VIX62_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-coenzyme
                     A--6-aminopenicillanic-acid-acyltransferase form"
                     /protein_id="HEY5859262.1"
                     /translation="MTWPIVVATGGPRERGRAYGQAARERVHGSIELYRAIFRHYTGL
                     TWDQVRGRAGAFVEPIDALDVQLLPEM"
     gene            329..1687
                     /locus_tag="VIX62_03150"
     CDS             329..1687
                     /locus_tag="VIX62_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5859263.1"
                     /translation="MPSIDRSRIATLMERERQTFRDRHPRSGELEEQAKPSLLFGVPM
                     NWMIRWPGDWPVFVDHAEGAHFTDVDGNTFVDFCLGDTGGMAGHGPKASVAAIAEQAA
                     KGITLMLPTEDAAWVGTEMTRRFKVPLWQFCLTATDANRFVLRWAREITGRPRVVVHN
                     WNYHGSVDETFAQLDDDGNLRPREGLIGQAAPLELTTKVVEINDLEALEAALAPGDVA
                     AVLFEPALTNIGIVLPDPGYHQGVRDLCTKYGTLLVIDETHTICAGPGGCTKAWDLDP
                     DLVTIGKTLGAGVPSAAFGMTQEVATRIIDHTDWRNSDVGGTGGTLAGNALSLAAMRA
                     TLGEVLTDEAFERMVGLGERFEEGVQGVIDSRELPWHVTRIGCRVEYLFRPDEAKNGA
                     QAAAGQDEELDTLIHLYMLNRGILMTPFHNMSLMSPATTAEDVDRHTQVFAEAADELV
                     QA"
     gene            complement(1715..2818)
                     /gene="serC"
                     /locus_tag="VIX62_03155"
     CDS             complement(1715..2818)
                     /gene="serC"
                     /locus_tag="VIX62_03155"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY5859264.1"
                     /translation="MSDRVVNFAAGPAILPQPVLEEVRRDLLSLPGVGASALELSHRG
                     PWFEGVIAEAEANLRTLLAIPASHQVLFCQGGATQQFSMVALNLPHGEDRAADLVITG
                     SWGTKAAKEAATTGAIRIAWTGEADGFRRVPMDAELAETLAPGAAYTHITTNETIQGV
                     EFTTDPATVGDVPLVADMSSDFLARPVDVARYGVVYAGAQKNLGPAGVTIAIVRDDVL
                     AQVPDGVPTMLDYRTFAEHGSMYNTPPVFSIYVSMLVTRWLLQEVGGLGAQHARNREK
                     AGLLYAAIDDSEGFYRGHADLGSRSLMNVTWRLPNEDLDKAFIAQAADEGLVELKGHR
                     SVGGIRASIYNAMPLEGVRALAGFMRGFAADRG"
     gene            complement(2871..3344)
                     /locus_tag="VIX62_03160"
     CDS             complement(2871..3344)
                     /locus_tag="VIX62_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859265.1"
                     /translation="MYRFWVFLHLVGMAGFLVSHGVSMAALFQVRAAGPDRDRILAIC
                     EGSKRMNRPMYLFTILLLVGGVAAGIDATSFDEFWLWGSLLILVATMGIMSFTATPYM
                     KRVREGCTRWADGTYTLSDDELDATLRGPMTMVVSLTGAVALVVILYLMVFKPGA"
     gene            3438..4457
                     /locus_tag="VIX62_03165"
     CDS             3438..4457
                     /locus_tag="VIX62_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5859266.1"
                     /translation="MAYKRILYGTDGTERAARSAHVATGLANAGKAQLIVAHVWERPD
                     GADQVLAKALADAEAAGVKRLEGELHGGRPPADVMIELAESRDVGLIVVSGGRSSISL
                     GPTADRLSHHSPRDLLIVMNTERGVTLEDGSERLYRRVLIATDGSATADRAARKGYDL
                     AESIGAPVTLVFVGHPATGRLVTEDTAAVYGVGVETDVVMKQGDPSDGIVEAAHEVDA
                     DLIVVGNKGMQGAKRFFLNPVPEKVLDLSDRDVLIARTVSQVASELQPGEGGIIVQAG
                     ERLAAFMDPKGKLHVMSAKCTHMGCTVAWNATTSLFECPCHGSKFEPSGEVANGPATR
                     PLPPA"
     gene            complement(4459..4941)
                     /locus_tag="VIX62_03170"
     CDS             complement(4459..4941)
                     /locus_tag="VIX62_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859267.1"
                     /translation="MYRYWFFLHILGVVAFMAAHGASMFTLFRVRNLNLDRAKIADAI
                     SFSGVTTRPMYVSLLVIVVAGIGSAITGPVGFNDWWLWLAIAILVVTIGLMTAIAAPY
                     FRKLTEACAVRPSGVPRTSDEELTALLRSATTWVITAIGTIGILVILYLMIFKPGNTF
                     "
     gene            complement(4972..5820)
                     /locus_tag="VIX62_03175"
     CDS             complement(4972..5820)
                     /locus_tag="VIX62_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY5859268.1"
                     /translation="MPIQDATSPLRRVYVRPPSAGALAAWRDYAWHAEPDVGAAADEH
                     AKLQATLQEAGAEVVLGSPVAGDPDSLYAYDPTLLTDAGVILLHPGKEGRRGEPEVMG
                     ADLEALGIPVIGRMQAPGTAEGGDMFWLDPTTLLIGRGYRTNDDGIAQLRAMLEPAGI
                     DVVGFDLPHFRGPGMCIHLMSFISPLDRDLAVVFPMHMPVRLMEILRMRGIAIVEVPE
                     EEFDSQGANVLALGPRVALAVDGNPETRRRMEAAGVEVRTFSGAEISVKGDGGPTCLT
                     RPLERG"
     gene            complement(5836..>6381)
                     /locus_tag="VIX62_03180"
     CDS             complement(5836..>6381)
                     /locus_tag="VIX62_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859269.1"
                     /translation="GPNSGPDTGPGSGDGQHGNGTGGEPDGPGTFYPIQPFAAAAIPL
                     NAIFPRNDGPPDLGVDPTIANPGTNGGPNDDHDPNHGNGNHGNNHGTKHHGGPKHTHG
                     TTTQKHADRTPGHSGEHNPHGGPPGLSGNGHGQGGEHSNAGGNGHGKANGHGNGHGNG
                     NGNGNGNGNGNGQSHEHGHGS"
BASE COUNT         1001 a         2187 c         2231 g          962 t
ORIGIN      
        1 tccatctccg gcagcagctg cacgtcgagg gcatcgatcg gttccacgaa ggcgcccgcg
       61 cgaccccgca cctgatccca ggtgagcccg gtgtagtgcc ggaagatcgc ccgatacagc
      121 tcgatcgaac cgtggacacg ctcgcgtgcc gcctgcccgt aggcgcgtcc gcgctcgcgc
      181 ggtccgcccg tcgccacgac gatcggccat gtcatcggtg ggcagcctac cccgctgaca
      241 gccgcgccgg ggctcgtcgt agactgtcga ccgttcggag accagcgctc gcgggaccgc
      301 cgtcccgcgg acaccaggag gaggaccgat gccgtcgatc gaccgttccc ggatcgccac
      361 gctgatggag cgagagcgcc agacgttccg agaccgccac ccccgatccg gagagctcga
      421 ggagcaggcc aagccctcgc tgctgttcgg ggtgccgatg aactggatga tccgctggcc
      481 gggcgactgg ccggtgttcg tggaccacgc tgagggcgcg cacttcaccg acgtcgacgg
      541 caacaccttc gtggacttct gcctcgggga caccggtggc atggcggggc acgggccgaa
      601 ggcctcggtc gccgcgatcg ccgagcaggc cgccaagggc atcaccctga tgctcccgac
      661 cgaggacgcc gcctgggtgg gaaccgagat gacgcgccgc ttcaaggtgc cgctgtggca
      721 gttctgcctg acggcgaccg acgccaaccg gttcgtcctg cggtgggccc gggagatcac
      781 cgggcgcccg cgggtcgtcg tgcacaactg gaactaccac gggtcggtcg acgagacgtt
      841 cgcccagctc gacgacgacg gcaacctgcg gccgcgcgaa gggctgatcg gtcaggccgc
      901 tccgctggag ctcaccacca aggtcgtgga gatcaacgac ctggaggcgc tggaagcggc
      961 cctcgccccg ggtgacgtcg ccgcggtgtt gttcgagccc gccctgacga acatcggcat
     1021 cgtgctgccc gatcccggct accaccaggg cgtccgtgac ctgtgcacga agtacggcac
     1081 gctcctggtc atcgacgaaa cgcacacgat ctgcgccggt cccggtggct gcacgaaggc
     1141 ctgggacctc gaccccgacc tggtgacgat cggcaagacg ctcggcgcgg gcgtgcccag
     1201 cgccgccttc ggcatgaccc aggaggtggc cacgcgcatc atcgatcaca ccgactggcg
     1261 gaactccgac gtggggggaa ccggggggac gctcgccggc aacgcgctgt cgctggccgc
     1321 gatgcgggcc accctgggcg aggtgctcac cgacgaggcg ttcgaacgga tggtgggcct
     1381 cggtgaacgg ttcgaggaag gcgttcaggg tgtgatcgac agccgggaac tgccatggca
     1441 cgtcacccgc atcggctgcc gcgtggagta cctgttccga ccggacgagg cgaagaacgg
     1501 cgcgcaggcc gccgcgggcc aggacgagga gctcgacacc ctgatccacc tctacatgct
     1561 gaaccgcggc atcctgatga ccccgttcca caacatgtcg ctgatgtcgc ccgccaccac
     1621 cgccgaggac gtggaccggc acacccaggt cttcgccgaa gcggcggacg aactggtgca
     1681 ggcgtaacgg ggacgatcgg cgaggagccg gggtctagcc gcggtcggcg gcgaacccgc
     1741 gcatgaaccc ggcgagggct cgcacgccct cgagcggcat cgcgttgtag atgctggcgc
     1801 ggatcccgcc gacgctgcgg tgccccttca gttcgaccag gccctcatcg gcggcctggg
     1861 cgatgaaggc cttgtcgagg tcctcgttcg gcaggcgcca cgtgacgttc atcagcgacc
     1921 gcgagccgag gtcggcgtgt ccccggtaga agccctcgct gtcgtcgatc gcggcgtaga
     1981 gcagccccgc cttctcgcgg ttgcgggcgt gctgcgcccc gagccccccc acctcctgca
     2041 ggagccagcg ggtcacgagc atcgagacgt agatcgagaa caccggcggc gtgttgtaca
     2101 tcgatccgtg ctcggcgaac gtccgataat cgagcatcgt gggcacgccg tcgggcacct
     2161 gcgccaggac gtcgtcccgc acgatcgcga tcgtgacgcc cgccgggccg aggttcttct
     2221 gcgcgccggc gtagaccacg ccgtagcggg ccacgtcgac cggccgggcc aggaagtccg
     2281 acgacatgtc cgccaccagc ggcacgtcgc cgacggtcgc gggatcggtc gtgaactcca
     2341 cgccctggat cgtctcgttc gtcgtgatgt gggtgtacgc ggcgccgggg gcgagcgtct
     2401 cggcgagctc ggcgtccatg ggcacccggc ggaagccgtc ggcctcaccc gtccaagcga
     2461 tgcggatggc tccggtcgtt gccgcttcct tcgccgcctt cgttccccac gaccccgtga
     2521 tcaccaggtc ggccgcccgg tcttcgccgt gggggaggtt gagggccacc atcgagaact
     2581 gctgcgtggc tccgccctgg cagaacagga cctggtgaga cgccgggatc gccagcaacg
     2641 tgcgcaggtt cgcctccgct tcggcgatca cgccttcgaa ccaagggccg cgatgcgaga
     2701 gctccagcgc cgaggccccc acgccgggca gcgagagcag atctcgccgg acctcctcga
     2761 gcaccggttg cggcaggatg gccggtccgg cggcgaagtt gacgacgcga tcgctcatga
     2821 ccgagccagg ctatcgacgc gggcggtgag cgtcggggac accatggcca tcacgcaccg
     2881 ggcttgaaca ccatgaggta caggatgacg accagcgcga ccgcgcccgt gaggctcacg
     2941 accatggtca tgggtccgcg cagggtggcg tcgagctcgt cgtcggacag cgtgtacgtg
     3001 ccgtcggccc accgtgtgca gccctcgcgc acccgcttca tgtagggggt ggcggtgaag
     3061 gacatgatgc ccatcgtggc gaccaggatg aggagcgatc cccacagcca gaactcgtcg
     3121 aaggacgtgg cgtcgatccc ggcggccacg ccgcccacga gcagcaggat cgtgaacagg
     3181 tacatcggcc ggttcatccg tttggagcct tcgcagatgg ccaggatccg gtcccggtcg
     3241 gggccggccg cgcggacctg gaacagcgcc gccatcgaga cgccgtggct cacgaggaag
     3301 cccgccatgc ccaccagatg caggaacacc cagaaccggt acatggtgcg agcatatcca
     3361 tgggcgcttc gggcccccgg cttcatgatc tgcgtctcgg gctggtagcg tggccgaccc
     3421 gaaaggacga acgacccatg gcgtacaagc ggatcctcta cggcaccgac ggcaccgagc
     3481 gcgcggcgcg gtccgcacac gtcgccacgg ggctcgccaa cgcgggcaag gcccaactca
     3541 tcgtggccca cgtctgggag cggcccgacg gcgccgacca ggtcttggcc aaagccctgg
     3601 ccgatgccga agccgccggc gtcaagcggc tggagggcga actccacggt ggtcgaccgc
     3661 ccgccgacgt catgatcgag ctcgccgagt cccgcgacgt cggactgatc gtggtgagcg
     3721 gtggccggag cagcatctcg ctcggtccga ccgcagatcg cctctcgcat cactcgcccc
     3781 gcgacctgct gatcgtgatg aacaccgagc ggggggtgac gctggaggac gggagcgagc
     3841 gcctgtaccg acgcgtgctg atcgcgaccg acggttcggc cacggccgac cgcgcggcgc
     3901 gcaagggcta cgacctcgcg gagtcgatcg gagcaccggt cacgctggtg ttcgtcggcc
     3961 atcccgcgac cgggcgcctg gtcaccgagg acaccgccgc ggtctacggc gtgggggtcg
     4021 agaccgacgt ggtgatgaag cagggcgacc cgtccgacgg gatcgtcgag gccgcgcacg
     4081 aggtcgatgc cgatctcatc gtcgtgggga acaagggcat gcagggcgcg aagcgcttct
     4141 tcctcaaccc ggtgcccgag aaggtgctcg acctgagcga tcgcgacgtc ttgatcgccc
     4201 ggacggtgtc ccaggtggcg tccgagctgc agcccggcga gggcgggatc atcgtgcagg
     4261 cgggggagcg gctcgccgca ttcatggatc cgaagggcaa actccacgtg atgtcggcca
     4321 aatgtaccca catgggctgc accgtggcgt ggaacgccac cacctcgctg ttcgagtgtc
     4381 cgtgccacgg ctcgaagttc gaaccgtcgg gcgaggtggc caacggaccc gccacccggc
     4441 cgctgccgcc ggcctagctc agaacgtgtt gcccggcttg aagatcatca ggtagaggat
     4501 caccaggatc ccgatcgttc cgatggcggt gatcacccac gtggtggccg atcgcagcag
     4561 ggccgtgagt tcttcgtcgc tcgtccgcgg cacacccgac ggccgcacgg cgcacgcttc
     4621 ggtgagcttg cggaaatagg gagcggcgat cgccgtcatc aagccgatcg tcacgaccag
     4681 gatcgcgatc gcgagccaca gccaccagtc gttgaacccg acgggcccgg tgatggccga
     4741 gccgatcccc gccaccacga tgacgagcag ggagacgtac atgggacggg tcgtgacccc
     4801 cgagaaggag atggcgtccg cgatcttcgc ccgatccagg ttgaggttcc gcacgcggaa
     4861 gagcgtgaac atcgacgccc cgtgggcggc catgaacgcg accaccccca ggatgtgcag
     4921 gaagaaccag taccgataca tcgcgcggga gcatatcgct ccctcgcgga ctcagcctcg
     4981 ttcgagcggg cgggtgaggc aggtggggcc gccgtcaccc ttcaccgaga tctcggcgcc
     5041 cgagaacgtg cggacctcca cgcccgcggc ctccatccgg cggcgggtct cggggttgcc
     5101 gtccacagcc agcgcgacgc gcggtcccag ggccagcacg ttggcgccct gcgagtcgaa
     5161 ctcctcctcg ggcacctcca cgatcgcgat gccgcgcatg cgcaggatct ccatcaggcg
     5221 cacgggcatg tgcatcggga agaccacggc cagatctcgg tccagcggtg agatgaacga
     5281 catcaggtgg atgcacatgc ccggcccacg gaaatgcggg aggtcgaagc ccaccacgtc
     5341 gatgccggcc ggctccaaca tcgcgcgcag ctgggcgatc ccatcgtcgt tggtgcgata
     5401 gccgcgaccg atcagcagcg tggtggggtc cagccagaac atgtcgccgc cctcggccgt
     5461 gccgggcgcc tgcatccggc cgatcaccgg gatccccagg gcctcgaggt cggcgcccat
     5521 cacctcgggt tcacctcggc ggccttcctt gccgggatgg agcaggatca cccccgcatc
     5581 ggttaagagc gtggggtcat aggcgtagag cgagtcggga tcaccggcga cgggcgaccc
     5641 aaggaccacc tcggcaccgg cctcctggag ggtggcctgc agcttcgcgt gttcgtcggc
     5701 ggccgctccg acatcgggct ctgcgtgcca ggcgtagtcg cgccaggccg ccagggcgcc
     5761 ggcgctggga ggtcgcacgt agacacgtcg aagcggtgag gttgcgtcct ggatcggcat
     5821 gcgtggatgg tacgactacg agccgtggcc gtgctcgtgg ctctggccgt tcccgttgcc
     5881 attgccattg ccattgccat tgccgttgcc gtggccattg ccgtgaccgt tcgccttgcc
     5941 gtgaccgttg ccgccggcgt tgctgtgctc cccgccttgg ccgtgcccgt ttccgctgag
     6001 cccgggaggt ccaccgtgcg ggttgtgctc gccactgtgg ccgggcgtgc gatcggcgtg
     6061 cttctgcgtc gtcgtcccgt gggtgtgctt cggcccgccg tgatgtttgg tgccgtggtt
     6121 gttgccatgg ttcccgttgc cgtggttcgg gtcatggtcg tcgttcggac cgccgttggt
     6181 gcccgggttc gcgatcgtcg ggtcgacgcc gagatcgggc ggtccgtcgt tgcgcgggaa
     6241 gatcgcgttg agagggatcg cggcggcggc gaacggctgg atcggataga acgtcccggg
     6301 gccatctggc tctccgccgg tgccgttccc gtgctggccg tcgccacttc cggggccggt
     6361 gtccggtccg ctattgggtc c
//
LOCUS       DATDBZ010000128         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_251577, whole genome shotgun sequence.
ACCESSION   DATDBZ010000128 DATDBZ010000000
VERSION     DATDBZ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251577"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VIX62_03185"
     CDS             <1..499
                     /locus_tag="VIX62_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEY5859270.1"
                     /translation="LAHTYTGFITDLRVNGENWNETHLNAALAGTFGVPVGFVAGDRV
                     CCEQAKERLPWIKTVTVKEGFGDRVGRSQSPTKARAAIRGILRDVVKNAERLEPWNPP
                     GPYTLELDLVNTAVADLCALSPGTDRTGPRTVAFTTDDFRVLYRCLLTWMHLGRSAAP
                     RSPVD"
     gene            complement(485..1603)
                     /locus_tag="VIX62_03190"
     CDS             complement(485..1603)
                     /locus_tag="VIX62_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5859271.1"
                     /translation="MRDELGRPFAEDYRELVEGIPAILYIDRPDEYSTNFYTSPQAEA
                     LLGYSSEEWGSDQDLWLRIIHPDDVDAVREENERSNIDGEPFHAEYRMLAKDGRIVWI
                     RDDALLVRDDLGAPKYWRGIMLDVSAEKDAEEKLRWSLDVLRRTIQQRRELAQRIETA
                     QEEERRQIAADIHDDPIQVMSAVDLRLAMLAERPETITTTALGELRSTVRQSIERLRS
                     LVFELRPAALDRDGLVVAIDQYLAHAAKEIGWTYEVVDQLDHEPDPELRVSLYRIVQE
                     AVANARKHAGARRVTVGVRTVGDGVTVIVADDGGGFDTDRVETPVPGHLGLATMIERA
                     ELVGGWCRVSSAADSGTTVQCWLPDDGVGDDGLASVHG"
     gene            complement(1606..>2095)
                     /locus_tag="VIX62_03195"
     CDS             complement(1606..>2095)
                     /locus_tag="VIX62_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5859272.1"
                     /translation="TVAMQELNGRWPGVTVNVGVARGGTRTNVVAERCVLQVDLRSPE
                     LETLEAAEAEIDRICAEHGIEGVTAHVDGNGWHRPMEKKEGGARLAALAIEIASELGF
                     ELRDAATGGASDANTTSAAGTPTIDGLGPIGGDDHAPAEWLDLTSVVPRIALLAGLIS
                     RV"
BASE COUNT          301 a          760 c          681 g          353 t
ORIGIN      
        1 cctcgcccat acctacacgg gtttcatcac cgaccttcga gtgaacggcg agaactggaa
       61 cgagacccac ctgaacgccg cgctcgccgg cacgttcggc gtgccggtgg ggttcgtggc
      121 cggtgaccgg gtgtgctgcg agcaagccaa ggagcgcctg ccatggatca agaccgtcac
      181 cgtcaaggag ggcttcggtg accgggtcgg ccggtcacag tcaccgacga aggcccgcgc
      241 cgcgatccgg ggcatcctgc gtgacgtggt gaagaacgcc gagcgcctcg aaccctggaa
      301 ccctcccggg ccttacaccc tcgagctcga ccttgtgaac accgcggtgg cggacctctg
      361 cgccctctcc cccggcaccg atcgaacggg gcctcgcacc gtcgctttca cgaccgatga
      421 cttccgggtg ctctaccgat gcctgctcac ctggatgcac ctggggcgca gcgcagcccc
      481 gcgttcaccc gtggactgag gccaggccgt cgtcaccgac gccgtcgtct ggcagccagc
      541 actgcaccgt cgttcccgag tcggcagcgc tcgacacgcg gcaccacccg cccaccagct
      601 ccgcccgctc gatcatggtc gcgagcccga ggtggccggg caccggcgtc tccactcgat
      661 cggtgtcgaa cccgccgccg tcatcggcga cgatcaccgt gacgccgtcg ccgacggtgc
      721 gaacgcccac cgtcacgcgg cgtgcgccgg cgtgcttgcg cgcgttggcg acggcttcct
      781 gcacgatgcg gtacagcgac acgcgcagct ctggatcggg ttcgtggtcg agctggtcga
      841 ccacctcgta ggtccacccg atctccttgg ccgcatgcgc gaggtactga tcgatcgcca
      901 ccacgaggcc gtcgcgatcg agcgcggccg gccgcagctc gaacacgagt gagcgcagcc
      961 gctcgatcga ctggcgcacc gtggatcgca gttcaccgag cgcggtcgtg gtgatcgtct
     1021 ccggtcgttc ggcgagcatc gcgaggcgca gatcgaccgc gctcatcacc tggatcgggt
     1081 cgtcgtggat gtcggcggcg atctggcgtc gttcctcctc ctgggcggtt tcgatgcgct
     1141 gggcgagctc acggcgttgc tggatcgtcc gccgcagcac gtcgaggctc cacctgagct
     1201 tctcctcggc atccttctcg gcggagacgt cgagcatgat gccgcgccag tacttcgggg
     1261 cgccgagatc gtcacgcacg agcagggcat cgtcgcggat ccacacgatg cgtccgtcct
     1321 tcgcgagcat gcggtactcc gcgtggaagg gctcgccgtc gatgttggag cgctcgttct
     1381 cctcccgcac cgcatcgacg tcgtcgggat ggatgatgcg aagccacagg tcctgatcgc
     1441 tgccccattc ctcgctggag tagccgagca gcgcttcagc ctgcgggctc gtgtagaagt
     1501 tcgtggagta ctcgtcgggc cgatcgatgt agaggatcgc cgggatccct tcgacgagct
     1561 ctcggtagtc ctcggcgaac gggcgcccga gctcgtcgcg catggtcaga ccctcgagat
     1621 cagacccgcg agcagggcga tgcgcggcac gacgctcgtg aggtcgagcc actcggcggg
     1681 cgcgtgatcg tcgccgccga tcggtccgag gccatcgatc gtcggcgtgc cggcggcact
     1741 cgtcgtgttc gcgtcgctcg ccccgccggt cgccgcatct cgaagctcga agccgagctc
     1801 cgacgcgatc tcgatcgcga gcgcggccag tcgtgcaccg ccctccttct tctccatcgg
     1861 gcgatgccac ccgttgccgt cgacgtgcgc ggtcacgccc tcgatgccgt gctcggcgca
     1921 gatacggtcg atctcggctt cggccgcctc gagtgtctcg agttcgggcg atcgcagatc
     1981 cacctgcaac acgcaccgct cggccaccac gttcgtgcgg gtcccgccac gcgccacccc
     2041 gacgttcacc gtgacccccg gccaccggcc gttgagctcc tgcatcgcca cggtg
//
LOCUS       DATDBZ010000129         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_659544, whole genome shotgun sequence.
ACCESSION   DATDBZ010000129 DATDBZ010000000
VERSION     DATDBZ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659544"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="VIX62_03200"
     CDS             <1..1481
                     /locus_tag="VIX62_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY5859273.1"
                     /translation="PVRSIRVGLTWDHSVMHVEAKSGPVHLWVGREGGRRVGRIPAGR
                     TWTVRARSSGFAVTDHDGKLVGGRTWGGTGFDLIATYGAGARAFVREAGYSYNRGSLE
                     FNLYKRGGSWNERLVLPIPFEQYLYGLGEMPSSWPAAALRAQAVAGRTFATYKVKRYA
                     PNRQCNCDITDGANDQVYVGYSKESGTDGNRWVAAVDDTRGQVVTHNGNVIQAFFAAS
                     DGGHSDSVEDVWHGGDPDFAIAYLKAECDPGEDTAANPWTDWQKSLTASELTSRLSPY
                     TGSIGIVRGFGAIRRGAGGRIIRAKVRGRSGSASVTGSELRWAIGAWDGRIWINEDKN
                     ITGPIRTRYDAVMCRPGLPTSRIQSVDGGSRQQFKKGAIFRTASAGVTVWLRGPVYRE
                     YRSIGGPNSPIGMPVSQIVDVDPGGGTRALFERGRILGKGGLGTHALWGRVLSEYLDR
                     SGTDGPLGFPTSRVRDDGSGGTLADFEHGTIVCPEGQGCRLA"
     gene            complement(1497..>2030)
                     /locus_tag="VIX62_03205"
     CDS             complement(1497..>2030)
                     /locus_tag="VIX62_03205"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY5859274.1"
                     /translation="TKAAGPGEIPQEAVAAAAAAGCTEVVQPVGDAPASQHVPDGTPI
                     TYAQRPATSGEHYAGRVLPSSPDSYDQPIDEPAAVHFLEHAGVMLYYRADGDGAVSPQ
                     VVDALQGVASAQQMTIAAPFDGLPDGTSVALAAWDQLQTCPGTITAGQATTIATGFSG
                     ALACTSNAPEANAADDC"
BASE COUNT          296 a          682 c          754 g          298 t
ORIGIN      
        1 gcccagtgcg gtcgatccgc gtgggcctca cctgggatca ctccgtgatg catgtggagg
       61 ccaagagcgg tcccgtgcac ctgtgggtcg gccgggaagg cggccggagg gtcgggcgca
      121 tccccgccgg gcgcacctgg accgtgcggg cgaggtcgag cggcttcgcc gtgaccgatc
      181 acgacgggaa gctggtcggt ggccgcacgt ggggcggcac cggcttcgat ctgatcgcca
      241 cgtacggcgc gggcgcccgc gcgttcgtgc gtgaggccgg gtactcctac aatcgtggct
      301 ccctcgagtt caacctctac aagcggggcg ggtcgtggaa cgagcggctc gtgctgccga
      361 tcccgttcga gcagtacctc tacggcctcg gggagatgcc gagctcctgg cccgccgccg
      421 cgctgcgcgc gcaggccgtg gcgggtcgca cgttcgccac gtacaaggtc aagcgttacg
      481 ccccgaaccg ccagtgcaac tgcgacatca ccgacggcgc caacgatcag gtgtacgtgg
      541 gctacagcaa ggaatcgggc accgacggga accgctgggt cgcggccgtc gacgacacgc
      601 ggggacaggt ggtcacccac aacgggaacg tgatccaggc gttcttcgct gcctccgacg
      661 gggggcattc cgactcggtc gaggacgtct ggcacggtgg cgaccccgac ttcgcgatcg
      721 cctacctcaa agcggaatgc gatcccggtg aagacacggc ggccaacccg tggaccgact
      781 ggcagaagtc gctgacggca tcggagctca ccagccgcct gtcgccgtac acgggttcga
      841 tcggcatcgt gcgcgggttc ggcgcgatcc gccgaggggc cggcggccgc atcatccgcg
      901 cgaaggtgcg ggggcggagc ggctcggcct cggtcacggg gtcggaactc cggtgggcca
      961 tcggcgcctg ggatgggcgc atctggatca acgaggacaa gaacatcacg gggccgatcc
     1021 gcacgcgcta tgacgcggtg atgtgccggc ccggcctgcc cacgtcacgc atccagagcg
     1081 tcgatggcgg ttcccggcag cagttcaaga agggcgcgat cttccgcacg gcgagcgcgg
     1141 gcgtcacggt gtggctgcgc ggccccgtct atcgggagta ccggtcgatc gggggaccga
     1201 acagcccgat cgggatgccg gtgtcgcaga tcgtcgacgt cgacccgggt ggcggcacga
     1261 gggcactctt cgagcgcggc cgcatcctcg ggaaaggtgg gctgggcacc cacgcgctct
     1321 ggggtcgggt gctctctgag tacctcgatc gcagcggcac cgacgggcca ctcgggttcc
     1381 cgacgagccg ggtccgtgac gacggctcag gcggcacgtt ggccgacttc gagcacggca
     1441 ccatcgtgtg ccccgagggg caggggtgcc ggctcgcctg agagagagcg ggtcgctcag
     1501 cagtcgtcgg ccgcgttggc ctcgggggcg ttcgaggtgc acgcgagcgc tccactgaag
     1561 ccggtggcga tcgtcgtcgc ctgcccggct gtgatcgtgc cgggacaggt ctgcagctga
     1621 tcccacgccg cgagggcgac cgaggtgcca tcgggcaacc cgtcgaacgg ggcggcgatc
     1681 gtcatctgtt gcgccgaggc aacgccctgt aacgcgtcga ccacctgggg tgacaccgcg
     1741 ccgtcaccgt cggcccggta gtagagcatc acgcccgcgt gctcgaggaa gtgcacggcc
     1801 gccggctcgt cgatcggctg atcgtaggaa tcgggcgagc tcggcagcac acggcccgcg
     1861 tagtgctcgc ccgaggtcgc cggccgctgg gcgtacgtga tcggcgtgcc atcgggcacg
     1921 tgttggctcg ccggggcgtc gcccaccggt tgcaccacct cggtgcatcc ggcggcggcc
     1981 gccgcggcga ccgcttcctg cgggatctca ccgggacccg ctgccttcgt
//
LOCUS       DATDBZ010000130         4439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_251670, whole genome shotgun sequence.
ACCESSION   DATDBZ010000130 DATDBZ010000000
VERSION     DATDBZ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251670"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..941
                     /locus_tag="VIX62_03210"
     CDS             75..941
                     /locus_tag="VIX62_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019070075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY5859275.1"
                     /translation="MGDESEWAYPGDGEGPVHEVLLRPFAVDPYTVTNAAFSRFVEAT
                     GHVSEAERDGWSFVFGGFLPDDFPDTRAVVGAEWWRQVFGADWRRPEGPGSSIEDRSD
                     HPVVHVSWNDATAYCAWSGTRLLTEAEWEYAARAGSRTPFPWGEDLEPGGVHMMNVFQ
                     GRFPSENTAEDGFSGTAPVGAFPANDFGLCNMTGNVWEWCEDWYDAAFYSRSPGSDPD
                     GPSSGTYKVMRGGSYLCHRSYCRRYRVAARQGSEPSSTTGNVGFRVATDYSPTTDAMR
                     RSGTSHISATNT"
     gene            complement(875..1450)
                     /locus_tag="VIX62_03215"
     CDS             complement(875..1450)
                     /locus_tag="VIX62_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY5859276.1"
                     /translation="MTTTRLEAFSDAVIAIVITILVLELKVPHEPELAALRELVPVFL
                     SYVLSCVYLGIYWTNHHHLRVLTEHVNGAILWANLHLLFWLSLVPFTTGWMGENDFAR
                     APTAVYGVNLLLAAIAYFILQTAIVAEEGHGSRLKAALGRDLKGKVSPALYVAGILLS
                     LLQPWLGLTLYVFVALMWLVPDRRIASVVGE"
     gene            1557..2660
                     /locus_tag="VIX62_03220"
     CDS             1557..2660
                     /locus_tag="VIX62_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859277.1"
                     /translation="MAEATRYGSTERLDAVVIGSGQAGLAAAYYLREAGKRIVILESN
                     ARVGDNWRSRWDSLHLFTPARHDGLPGWPFPAPGWSFPAKDEMGDYLEAYVERFSLPV
                     ELETRVSDVHARDGHFVVSAEGRSFDADNVVVASGGFETPFVPAFAHELDDGIVQLHS
                     TGYRNQTQLQPGGVLVVGAGNSGAEVAIESAGGHATWLSGRHPGHIPFRMEGTASRLV
                     LERLVLRFVFHRVLTTGTPMGRKMKPKLTAKGTPLIRLKPKDIDAAGVETVARVAGVR
                     DGLPVLDDGRVMEVTNVVWCTGFRPDFSWIGLPGVGEGTPDHDRGIVGASPGLYFLGL
                     DFLYSLSSVMIHAADRDAHHVVDHIVRRRTSSS"
     gene            complement(2662..3849)
                     /locus_tag="VIX62_03225"
     CDS             complement(2662..3849)
                     /locus_tag="VIX62_03225"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY5859278.1"
                     /translation="MEPKKVWDGYRSFSYLDEGDVRRFELAREIGRVEPFVYPLGAED
                     DARAERLLRELVCISLHEHGGIAPADWADNDAYIREGREWYGYEGLAASGLDAVFENF
                     LDGTATITSRMGWKWTDVIHDLGMHFADVAHQSVVFVASTLDDIRRAHETGRIAMVPC
                     LEGAAPIENELDRLDVLYGLGVRMIGITYSESNQLGGGIRDPGDAGLTRFGLDAVRRM
                     NQLGLAIDVSHTGDLTALQTCEASQAPVFLSHSGARALFPERKLKTDDLLRAVAGTGG
                     VVGVEASPHTTITSSHPRHDLEGVMEHVEYCIDVMGIDHVGFGLDTFFGDHVGLHRAY
                     GAFMDSGEEPEHEWVDHVEGLENVSEFPNVIRWLVGHDYSDEHIAQLVGGNALRALGE
                     TWT"
     gene            4082..>4439
                     /locus_tag="VIX62_03230"
     CDS             4082..>4439
                     /locus_tag="VIX62_03230"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859279.1"
                     /translation="MVTTHAAIADPGQLDPTFSNDGRIILRDARAVEVRLQADGKVLV
                     VDANGEVRRYTGMGTLDRTYGGGDGKVQTDVAAIAAETRADGSLLVMGSMAGSVSFGL
                     ARLTPGGAFDRSFGGDG"
BASE COUNT          677 a         1465 c         1592 g          705 t
ORIGIN      
        1 gcccggtcac gacgagcgcc ggccgaggcg agcatccctc gacgctgacg gcgctcgagg
       61 gtgggcggtt cgcgatgggc gacgaatcgg agtgggccta cccaggcgac ggagagggac
      121 ccgtgcacga ggtgctcctg cgtccgttcg ccgtcgaccc ctacacggtg accaacgcgg
      181 cgttctcgag gttcgtcgag gccaccggcc atgtgtcgga ggccgagcgc gatgggtggt
      241 ccttcgtctt cggcgggttc ctgccggacg atttcccgga cacgcgagct gtggtcggcg
      301 cggagtggtg gcgccaggtc ttcggtgccg actggcggcg tccggaaggc ccgggttcct
      361 cgatcgagga tcgctcggac cacccggtgg tgcacgtgtc gtggaacgac gccacggcct
      421 attgcgcgtg gtcgggcacc cgtctcctca ccgaggcgga atgggagtac gcggcaagag
      481 ccggctcgcg caccccgttc ccgtggggcg aggacctcga accgggcggc gtgcacatga
      541 tgaacgtctt ccaggggcgg ttcccgtcgg agaacacggc tgaggacggc ttctccggca
      601 cggctccggt cggcgccttc ccggccaacg acttcggctt gtgcaacatg accgggaacg
      661 tgtgggagtg gtgcgaggac tggtacgacg cagccttcta ctcgcggagc cctggttcgg
      721 acccggacgg tccctcgagc ggcacctaca aggtcatgcg gggtggttca tacctctgcc
      781 accggtcgta ctgccgcagg taccgcgtcg ccgcccgaca gggttcggag ccatcgtcga
      841 cgaccggcaa cgtcgggttc cgggttgcca ccgactactc gcccacgacg gacgcgatgc
      901 gacgatcggg cacgagccac atcagcgcca cgaacacgta gagggtcagg ccgagccacg
      961 gctgcagcaa cgagagcagg atgcccgcca cgtagagcgc gggcgagacc ttgcccttca
     1021 gatcgcggcc cagcgcggcc ttgagccggg agccgtgccc ctcttccgcg acgatcgccg
     1081 tctgcaagat gaagtaggcg atcgcggcca gcagcaggtt cacgccgtac acggcggtcg
     1141 gcgcacgggc gaagtcgttc tcgcccatcc agccggtcgt gaacggcacg agcgagagcc
     1201 agaacagcag atgcaggttc gcccacagga tggcgccgtt cacgtgctcg gtgagcacgc
     1261 gcaggtggtg gtggttggtc cagtagatgc cgaggtacac gcagctcagc acgtagctca
     1321 ggaagaccgg caccagttcc cgcagggccg ccagctcggg ctcgtgcggc accttcagtt
     1381 ccagcaccaa gatcgtgatc acgattgcga tcacggcgtc gctgaaggcc tcgagccgcg
     1441 tggtcgtcat ggcagcagat gatgcaccat cggcgacgct cgcgtcgtct gtggaccggc
     1501 ccttcccgga tgtgtcatgg tcgcgcggag cagcgctcgg cgggccggga gggacgatgg
     1561 cggaggcgac gaggtacggg agcaccgagc gactcgacgc cgtcgtgatc gggagcggcc
     1621 aggcaggttt ggccgccgcc tactacctgc gagaggccgg caagcgcatc gtcatcctcg
     1681 agtcgaacgc ccgcgtcggc gacaactggc gatcgcggtg ggattcgctg catctgttca
     1741 cgccggcgcg acacgacggt cttcccggct ggccgttccc tgccccgggc tggtcgttcc
     1801 ctgccaagga cgagatgggt gactacctcg aggcgtacgt cgagcgcttc tcgttgccgg
     1861 tggaactcga gacgcgggtg agcgacgtgc atgcacgtga tggccacttc gtcgtctcgg
     1921 ccgagggccg ctcgttcgat gccgacaacg tcgtcgtggc atcgggtggg ttcgagacgc
     1981 cgttcgtgcc ggccttcgcc cacgagctcg acgacggcat cgtgcaactg cattcgacgg
     2041 gctaccggaa ccagacgcag ctgcagcccg ggggcgtgtt ggtggtcggc gccggcaact
     2101 cgggcgccga ggtggccatc gagtcggcgg ggggtcacgc gacctggctg tccggtcggc
     2161 acccggggca catcccgttc cgcatggagg gcacggcgtc gaggctcgtg ctcgagcgtc
     2221 tggtgctgcg gttcgtcttc caccgtgtgc tgacgaccgg cacgccgatg ggacggaaga
     2281 tgaagcccaa gctcacggcg aagggcactc cgctgatccg cctcaagccg aaggacatcg
     2341 acgcggctgg agtggagacc gtggcgcggg tcgccggcgt tcgcgatggc ctgccggtgc
     2401 tcgacgacgg ccgggtcatg gaggtcacga acgtcgtctg gtgcacgggc ttccgccccg
     2461 atttctcctg gatcggcctg cccggcgtgg gggagggcac gcccgatcac gaccggggca
     2521 tcgtcggggc ctcgcccggg ctctacttcc tcggccttga cttcctctac tcgctctcct
     2581 ccgtcatgat ccacgccgcg gatcgagatg cgcatcacgt cgtggaccac atcgtgcggc
     2641 ggaggacgag ctcgtcctga actaggtcca ggtctcgccg agagcccgaa gggcgttgcc
     2701 cccgaccagt tgcgcgatgt gctcatcgga gtagtcgtgc ccgacgagcc accggatcac
     2761 gttcgggaac tcgctcacgt tctcgagtcc ctcgacgtgg tcgacccatt cgtgctcggg
     2821 ttcctcgccg ctgtccatga aggcgccgta cgcgcgatgc agcccgacgt ggtcgccgaa
     2881 gaacgtgtcg aggccgaacc cgacgtggtc gatgcccatc acgtcgatgc agtactccac
     2941 gtgctccatc acgccctcga ggtcgtggcg cgggtggctc gaggtgatcg tggtgtgcgg
     3001 cgaggcctcg acacccacca cgccgccggt gccggcgacc gcccgcagca ggtcgtcggt
     3061 cttgagcttg cgctcgggga acaacgccct cgcgcccgag tgcgacagga agaccggagc
     3121 ctgtgacgct tcgcatgtct gcagagccgt caggtcgccg gtgtgggaga cgtcgatcgc
     3181 gaggcccagt tggttcatgc gccgcaccgc gtcgagcccg aaccgcgtga ggccggcgtc
     3241 gccggggtcg cggatgccac cgcccagttg gttcgactcg ctgtaggtga tgccgatcat
     3301 gcgcacaccg agcccgtaga gcacgtcgag ccggtcgagc tcgttctcga tcggcgcggc
     3361 gccctcgagg cagggcacca tcgcgatgcg gccggtctcg tgggcgcgcc ggatgtcgtc
     3421 gagcgtggag gccacgaaca cgacggactg gtgcgcgacg tcggcgaagt gcatgccgag
     3481 gtcgtggatc acgtcggtcc acttccagcc catcctcgag gtgatggtgg ccgtgccgtc
     3541 gaggaagttc tcgaagaccg cgtcgagacc cgaggcggcg agtccctcgt agccgtacca
     3601 ctcccggccc tcccggatgt aggcgtcgtt gtcggcccag tccgccggcg cgatgccgcc
     3661 gtgctcgtgc aacgagatgc acacgagctc acgcaggagc cgttcggcgc gcgcgtcgtc
     3721 ctcggccccg agcggataca cgaacggctc gacgcgcccg atctcgcgcg ccagctcgaa
     3781 ccggcgcacg tcgccctcgt cgaggtagga gaacgaccgg tacccgtccc agaccttctt
     3841 cggctccatc gccgagaggc taccgccggg gggcggagcc gtatggaagg aggggcgcgc
     3901 gggtccttcc gatgatggga cgaacgcgga cgttcccgct gcccgccacc gccatgctgg
     3961 cctgagaccg cggggaaggg agcacatcat ggccgttcgg gggcagtcga gagcgcgggg
     4021 ggcgggaaga cgcgtgccgg tgcaccggat catggcgctc tgtctagctg tcgcgttcgc
     4081 catggtcacg acgcacgcgg cgatcgccga cccgggccag ctcgacccga cgttcagcaa
     4141 cgacggtcgc atcatcctgc gcgatgctcg agcggtggaa gtccgactgc aggccgatgg
     4201 caaggtcctg gtggtcgatg cgaacggcga ggtccgtagg tacacgggta tggggacgct
     4261 cgacaggacc tacggcggcg gggacggcaa ggtccagacc gacgtcgcgg cgatcgcggc
     4321 tgaaacccgt gcggacggat cgctgctggt gatgggctcc atggcgggtt cggtctcgtt
     4381 cgggctggca cgcctgacgc ccggcggcgc cttcgaccgg tccttcggcg gtgacgggc
//
LOCUS       DATDBZ010000131         8469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1046605, whole genome shotgun sequence.
ACCESSION   DATDBZ010000131 DATDBZ010000000
VERSION     DATDBZ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046605"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..515
                     /locus_tag="VIX62_03235"
                     /pseudo
     CDS             69..515
                     /locus_tag="VIX62_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513040.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
     gene            732..1574
                     /locus_tag="VIX62_03240"
     CDS             732..1574
                     /locus_tag="VIX62_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5859280.1"
                     /translation="MGYITVGDENSTPIELYYEDQGSGQPVVLIHGYPLNGHSWEKQT
                     RELLAAGYRVITYDRRGFGQSSKVASGYDYDTFAADLNTLLETLDLRHVILVGFSMGT
                     GELARYVSRYGHERVAKLAFLASLEPFLLQRDDNPEGLPQAGFDEIAAAAKADRNAWF
                     TQFYKGFYNLDETLGSRITEEVVTGSWNVAITSAPVAAYAVVPSWLEDFRGDVEAVRA
                     AGKPALILHGTKDNILPIDATARRFREALPEADYVEIEDAPHGLLWTHADEVNSALKD
                     FLDK"
     gene            1722..2399
                     /locus_tag="VIX62_03245"
     CDS             1722..2399
                     /locus_tag="VIX62_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HEY5859281.1"
                     /translation="MDDGHTLAEDLLLLLFQPRSGTIAGEGTLFYVLAGAILADLGLG
                     DHVRTGSGRLGGLTVEAVADNPPSDHLLRVTWEYVADKPRGVQTVLAATGPSLREPLL
                     DRLIERGELRRHARKSLGFIETSVIEEGDSGRRDMLLDDIRAVLVDGADPAPRVAALA
                     ALLYGSGTLPQFDRDIPWTSPVISRAEELKDGNWGAGATAKAVARSVTSIIVSNVIVA
                     AAVLPRG"
     gene            2431..2611
                     /locus_tag="VIX62_03250"
                     /pseudo
     CDS             2431..2611
                     /locus_tag="VIX62_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558293.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(2709..3458)
                     /locus_tag="VIX62_03255"
     CDS             complement(2709..3458)
                     /locus_tag="VIX62_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007388709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5859282.1"
                     /translation="MNRFEGKTAVVTGGGTNGIGRAAAARLVDEGAHVFIMGRRENEL
                     NEAVAAIGRNVTAVPGDITKHSDLERLFGAVEAHGKGLDVLFANAGVATFAKIDDLTE
                     EDLNHVFGINVVGTMLTVQKAIPLLNTGGSVIINASTSADRGAPSMGAYAASKAALRS
                     FTRTWANELKDRGIRVNAISPGPTDTSGVVELVGAENVAAYNESLASSLPIGRTARLD
                     EMAGVVAFLASADSSFMLGANVLVDGGVNQI"
     gene            3553..4152
                     /locus_tag="VIX62_03260"
     CDS             3553..4152
                     /locus_tag="VIX62_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017564513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5859283.1"
                     /translation="MEQVRTGRPRSFDADVALEQALMAFWEHGYEGASLAVLTESMGI
                     SRKSMYAAFGNKEELFLKVLQRYEQGPGAYVHDSLHAPTAREVAMSFLDGAVSAGTQP
                     GRPAGCLGVRAALAVGVTGQAVQEILTSWRAKRQVDLRERFERAVDEGDLPSDADADM
                     IARYLTTVADGVSVQATEGASSASLRRVVDAALLHWPPA"
     gene            complement(4190..6076)
                     /locus_tag="VIX62_03265"
     CDS             complement(4190..6076)
                     /locus_tag="VIX62_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017589937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY5859284.1"
                     /translation="MQEQLAQDESPRPGVALPSGWKVARSVRYVTLLLLTTATATVLG
                     WLNRTPWWGWVIAITLIVGLAVVLRRLRRRSVLLRLGAWLLGTALTCATIVLAYPPPG
                     TRAAGGGDAASTQEVSTREGPVRGVVNDARTVEIFAGIPYAQPPIGDLRWRAPTPPEA
                     HGDVFVADRFSAVPIQATSTFFNRALSQVVDAPLEGTLLNPYRVSEDSLSLNIWRSTA
                     PASDALPVLVYVPGGGFTTGSGALPLYDGEALAARGEVITVTINYRLGVLGFLSHPDL
                     AEETGDDASGNFGILDQIAALEWIRDNIASFGGDPDRVTIAGESAGGQSVCILGASPL
                     SDGLVHGIIGGSGACMGTTGSTEKGDQFDTREAAEDAGRRLSERLGGATIDEMREMPI
                     DRLLDAAADLDSHWRPSVDGHVLPTSPVEIYASGRQHDVPILVGSNADEASLALALPP
                     EVDVDEYRSSARETYGELAETFLELYPGDTPEEALESSMQAQTDRVMTRAMLRWARLQ
                     TQSGDADAYLYFFSQTPPETGLQRYGAYHGAEVAYAYGNLGADHDADYTEADYLLRDQ
                     MSGYWVAFARTGDPNAPTLPAWPTVESAADRVMEFSVDGSRMTDRPRADAIDFWLEYE
                     GPIP"
     gene            6397..6561
                     /locus_tag="VIX62_03270"
     CDS             6397..6561
                     /locus_tag="VIX62_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5859285.1"
                     /translation="MRASQINGCACLDLHSREARQSGPPQQTLDLLPAWRETEVFSER
                     GCVREMPMGS"
     gene            complement(6569..7186)
                     /locus_tag="VIX62_03275"
     CDS             complement(6569..7186)
                     /locus_tag="VIX62_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5859286.1"
                     /translation="MTDVPNEQPPEAGPRPVGRPRRSQQDLLAAAAAVFVESGVDAPV
                     REIAAKAGVGMGTIYRHFPTRPALVVAVYRHQVEACAEAGPRLLTESATAEAALRAWV
                     ALFVDFLVTKHGLADALRGDNAGSDALHAYFVDRLVPVCGSLLEAARAERGLPDQELV
                     LNGYSLLKGIGNLCIGAAGDSGYDADRLVQALVSGALAGPATRQV"
     gene            7303..8247
                     /locus_tag="VIX62_03280"
     CDS             7303..8247
                     /locus_tag="VIX62_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019746915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorophyllase"
                     /protein_id="HEY5859287.1"
                     /translation="MTSAIVHALDGIVGAGTPVVSAAPVTLSAPDRPVPLEVRVSAPA
                     TGTNLPVVVFSHGNGWNLDGYSPLTAFWASRGFVVIQPTHLDSRRNGFGFEHPVFPTI
                     WTERIADLTRTLDQLDTIEGAVPGLAGRVDRSRVAATGHSWGGQTAQSLLGARILDEE
                     GNVGEDMSDHRVTAGILFAATGLGGDDLHPFAKANFPFMRPSFQELTTPTLVVAGDCD
                     QSKMSSRGPDWFTDAYTHSPGATDLLTFYGAEHALGGIVGYEVAETTDENPERVAVIQ
                     RLSTAYLRTALHVDENSWGAARAAVRDGADPIGRVDSK"
BASE COUNT         1392 a         2891 c         2855 g         1331 t
ORIGIN      
        1 aggagccccg cgacgaaccg ttcactcgct cgtcagagcg gagcgtccgg cgccggatga
       61 gcgcgtgtct ggccatgatg gcggccactt tggccaaccg gggcgtcaac ccgctcaccg
      121 gcgtggaggt ggtcgccgga cggggcgtgc ggcaggtgtt gagcgtgatg gccacccgcg
      181 gtatgtacga cgccgccggt gactgggcga ctcaggtcgg catcccggcg aagagcggtg
      241 tgtccggcgg gctcatcggc gctgcccggc caggtcggca tcgcgacctt cccaccgcga
      301 ctggacatta tgggaacagt gtgcgcggat gtcactgttc gagcgtttct ccgcctacac
      361 gagtctgcat gtgatggagg ttccggctgt cgcgcgtgcg gtcgtgcgat ccaaccacgt
      421 cgtgaggagc acgccggggg gaggtgcgcg ttctccagct gcaaggcggg atccgcttcg
      481 ccggagccga gcgcatcgac cgcgaagtcg tggacgacct cgaacaggcc gtgagcgatt
      541 cgcgagagcg accgagagct cgctcgagcg cggtggggcc ggcgggcacg ttctgcgtcg
      601 tgtcctgcga cgacccggct gaccgcaaga tcggggcgga gaacgctctt gaccgtgttg
      661 cgaccacacg gtgtgtgctg acagagagcg gccggtaacc ccgccggccg gagttgaaag
      721 gtcagaggat catgggttac atcaccgtcg gtgacgagaa cagtacgccg atcgagctct
      781 actacgagga ccaggggtcc ggtcagcccg ttgtgctgat ccacgggtac ccgcttaacg
      841 gtcacagctg ggagaagcag acgcgcgagc ttctcgcggc gggctatcgg gtcatcacat
      901 acgaccgtcg gggattcggg cagtcctcga aggtcgcctc cggatacgac tacgacacct
      961 tcgcggcgga tctgaacaca ctcctggaga ccctggatct gcggcacgtg atcctggtcg
     1021 gattctcgat gggcaccggc gagctggcca ggtacgtctc ccggtacgga cacgagcgcg
     1081 tcgcgaagct ggcgttcctg gcctcgctgg agccgttcct gctgcagcgc gacgacaacc
     1141 cggaggggtt gccgcaggcg gggttcgatg agatcgccgc ggctgcgaag gcagaccgga
     1201 atgcgtggtt cacgcagttc tacaagggct tctacaacct ggacgagact ctcggatcgc
     1261 ggatcaccga ggaggtcgtc accggaagct ggaacgtcgc gatcacgagc gccccggtcg
     1321 ctgcctacgc ggtcgtcccg tcgtggctcg aagacttccg cggcgacgtc gaggcggtcc
     1381 gcgccgcggg caagccggcc ctgatcctgc acggcaccaa ggacaacatc ctgcccatcg
     1441 atgccaccgc ccgccgcttc cgtgaggcgc tccccgaggc cgactacgtc gagatcgagg
     1501 acgctcccca cggcctgctg tggacccacg ccgacgaggt caacagcgcg ctgaaggact
     1561 tcctcgacaa gtgagcaccc cggtgcggag ggcagccggt gctgccctcc gcactcggcg
     1621 acaggagaac aatccgtgca gagtaaaggg catgagaaat gactgatcag caccgagacc
     1681 cgcgcgcttt cccccctgaa cagcccgacc gtgccggcgg cttggacgac gggcacacgc
     1741 tggcagagga cctccttctg ctgctcttcc agccccgatc cggcacgatc gccggagaag
     1801 gaacgctctt ctatgtgctc gccggggcga tcctcgccga cctcgggctc ggggatcatg
     1861 tgcgaaccgg ctcgggacgg ctgggagggc tgaccgtcga ggccgtcgcc gacaaccccc
     1921 catcggatca ccttctgcgg gtcacatggg agtatgtcgc cgacaagccc cgaggggtgc
     1981 agacggtcct ggcggcgaca ggaccgtctc tgcgcgagcc gctgctggat cgactcatcg
     2041 agcgggggga gcttcgtcga cacgcgcgca agagcctcgg cttcatcgag acctcggtga
     2101 tcgaagaagg ggactccgga cgccgggaca tgctgctcga cgacatccgc gcggtgctcg
     2161 tcgacggagc ggatcccgcg ccccgggtcg cggcccttgc cgcactgctc tatggcagcg
     2221 gcacactgcc acagttcgat cgggacatcc cttggacgtc tccggtgatc tcccgagcag
     2281 aggagctcaa ggacgggaac tggggagcgg gagccacggc gaaggcggtc gcccgcagcg
     2341 tcacctcgat catcgtcagc aacgtcatcg tcgcggcggc cgtgctgccg cgcggatgac
     2401 ggcacgaacg cacccgacag gaggctcagc ctgaccacgg tccatcaccg aaccatcacc
     2461 gtccaggggt tggaggtctt ccgccgcgag gccggccgaa agaggcgccg gcgatgcttc
     2521 tgctgcacgg tatccgacaa gttcgcacat cttccggcac ctcatcccgg cgctcgcccg
     2581 gcggtatcga gtgatcgctc cgtgccacct tctgacaggc ccggcgggcg cgtcgtcgcg
     2641 acgaagccga tcttcgagac cgggcttcgc cgcgacgcgc ggttccgagc gcagatcgcg
     2701 tcccaggctc agatctggtt caccccaccg tccaccagca cgttcgcacc cagcatgaag
     2761 ctgctgtcgg cggacgcgag gaacgccacg acgcccgcca tctcatcgag acgcgcggtg
     2821 cgtccgatcg ggaggctgga ggcgagactc tcgttgtagg ccgccacatt ctcggcgccg
     2881 acgagctcga cgacgccgga agtgtcggtc ggaccgggcg agatcgcgtt gacccggatg
     2941 ccacgatcct tgagttcgtt cgcccatgtg cgcgtgaagc tgcgcagggc cgctttcgat
     3001 gccgcatagg cccccatgct cggcgcccca cggtcagcgg atgtcgacgc gttgatgatc
     3061 acggaacctc cggtgttgag gagcggaatc gccttctgca ccgtgagcat cgtgccgacg
     3121 acgttgatgc cgaacacgtg attgaggtct tcctcagtga gatcgtcgat cttcgcgaac
     3181 gtcgcgacac cggcgttcgc gaagaggacg tccaggccct tgccgtgggc ctcgaccgct
     3241 ccgaacagcc gctcgagatc ggagtgcttc gtgatgtcgc cggggacggc ggtcacgttg
     3301 cggccgatcg cggcaacggc ctcgttcagc tcgttctcgc gacgccccat gatgaagacg
     3361 tgcgccccct catcgacgag tcgcgcggcg gcggcgcgac cgatcccgtt cgtgccacca
     3421 ccggtgacga ctgcagtctt gccctcgaac ctgttcacta ctcgtccctt cgtacacttc
     3481 agcaccgatc ggtgctgaaa tgaccctatc aacttgtgca ccgatcggtg gcaaagtgat
     3541 agagttcctg tcgtggagca ggtgagaacc ggtcggccgc gatccttcga cgccgatgtc
     3601 gcgctggagc aggcgctcat ggcgttctgg gagcacggct acgagggggc gagtctcgcc
     3661 gtgctcaccg agtcgatggg catctcgcgc aagagcatgt acgccgcgtt cggcaacaag
     3721 gaggaactgt tcctcaaggt gctccagcgc tacgaacagg gcccgggtgc ctacgtgcat
     3781 gattccctgc acgcccccac cgcgcgcgag gtcgccatgt cgttcctcga cggcgcagtg
     3841 agcgccggca cgcaaccggg ccgacccgcg ggatgcctgg gtgtgcgcgc ggctctggcg
     3901 gtcggagtca ccggccaggc cgtgcaggag atcctcacgt cctggcgcgc caagcgtcag
     3961 gtcgacctgc gtgaacggtt cgagcgggcc gtcgacgagg gtgatctccc ctcggacgcg
     4021 gatgccgaca tgatcgcccg ctacctcacg accgtcgccg acggcgtgag cgtgcaggcg
     4081 acggaagggg catcttcggc gtccctgcgg cgcgtggtcg acgcggcact tctgcattgg
     4141 ccgccagcgt gacagcagcg gatcgatcat gcggacgctt cggggatgat catgggatag
     4201 gcccctcgta ctccaaccag aagtcgatgg cgtcggctcg cgggcggtcg gtcatccggc
     4261 tgccgtccac gctgaactcc atgacccggt cggcggcgct ctcgacggtc ggccaggctg
     4321 gaagcgtcgg cgcgttgggg tccccggtgc gcgcgaacgc cacccagtag ccgctcatct
     4381 ggtcgcggag aaggtagtcg gcctcggtgt agtcggcgtc gtggtcggcg ccgagattgc
     4441 cgtaggcgta ggcgacctct gcaccgtgat aggccccgta ccgctgcagg cccgtctccg
     4501 gcggtgtctg cgagaagaag tacaggtacg cgtcggcatc gccggactgc gtctgcaatc
     4561 gcgcccaccg gagcatcgcg cgggtcatga cgcggtcagt ctgtgcttgc atcgacgatt
     4621 ccagcgcctc ctccggagtg tcgccggggt acagctcgag gaacgtctcg gcgagttcac
     4681 cataggtctc acgcgcggaa gaccggtact cgtcgacgtc cacttcgggc gggagtgcga
     4741 gcgcaaggga tgcctcgtcg gcattgcttc caacgaggat cggcacgtcg tgctgccgtc
     4801 ccgaggcgta gatctcaact ggggacgtcg gcagaacgtg cccatcgaca gacgggcgcc
     4861 aatgggaatc gaggtctgcc gcggcgtcga ggaggcgatc gatcggcatc tcccgcatct
     4921 cgtcgatcgt cgctccgccc aagcgttcgc tcaagcggcg gcctgcatcc tcagctgcct
     4981 cacgggtgtc gaactggtcg cccttctcgg tcgacccggt ggtgcccatg cacgcaccgc
     5041 ttccgccgat gatgccgtgc acaaggccgt cggagagcgg gctcgcgccg aggatgcaca
     5101 cactctgacc gccggccgac tcgccggcga tggtgacgcg atccgggtcg ccgccgaagg
     5161 aggcgatgtt gtcccgtatc cactcgagcg cggcgatctg gtccaggatt ccgaaattgc
     5221 ctgaggcgtc gtccccggtc tcctcggcca ggtccggatg agacaggaag ccgagcacgc
     5281 ccagccgata gttgatggtg actgtgatga cctctccgcg agcagcgagg gcctctccgt
     5341 cgtagagcgg cagggcgccg gaaccggtcg tgaagccgcc gccgggaacg tacacgagca
     5401 caggaagcgc gtcggatgcc ggcgcggtgc tccgccagat gttgagggag aggctgtcct
     5461 cactcacgcg atacgggttc agcagggtgc cctcgagcgg ggcgtccacg acctgtgaaa
     5521 gcgccctgtt gaagaaggtg gacgtcgcct gaatgggcac cgccgagaag cggtcggcga
     5581 cgaacacgtc gccgtgcgcc tcaggaggcg ttggagcgcg ccagcgcaga tctccgatcg
     5641 gcggctgggc gtacgggatg cccgcgaaga tctcgaccgt ccgcgcatcg ttcacgacgc
     5701 cccgcacggg gccttcgcgg gtcgagacct cttgcgtcga cgccgcatcg ccgccgcccg
     5761 ccgcccgcgt gcccggcggc ggataggcga gcacgatcgt cgcgcacgtg agggccgtgc
     5821 cgagcagcca cgcacccagc cgcaagagga ccgaccgtcg ccgcagccgg cgcaggacga
     5881 cggcgaggcc cacgatgagg gtgatggcga tcacccatcc ccaccagggg gtccggttga
     5941 gccagcccag aacggtcgcg gtcgcggtgg tcagtaggag aagggtcacg taccgcaccg
     6001 accgcgcgac cttccagcct gacggcagcg caacgcccgg ccgaggggac tcatcctgcg
     6061 ccaactgctc ctgcatgctt ccagatccct gcctctgttg cgttcgacgg gcgatccgtg
     6121 cccaccaccc gggacgagtc gaacacctgt gcccgcaaca gggtcaagcc gccgatgcgg
     6181 cacccgcact caccacggtt gaccgcgttg cgggaacagg gtcttgaatg ctggcacgga
     6241 cggtcacccg gatgatcgtc agagaagagg acgcgtgcgc ccgttcctgg acaaggtgat
     6301 gtccgaggtc tggcaggctg cagtggcgtt ctcgtccgcg gtgcgcgaag gggccacgca
     6361 gcggggactc tcggcgcagg agcggaactc atcaaggtgc gggcgtcgca gatcaacggc
     6421 tgcgcgtgtc tggatctgca ttcgcgcgag gcgaggcaat ccggaccccc gcagcagact
     6481 ctggatctgc tgccggcgtg gcgggagacc gaggtcttca gtgaacgtgg gtgcgtccgc
     6541 gagatgccga tgggaagctg acggagaatc acacctggcg agtagcgggg ccggcgagtg
     6601 cgccggacac gagcgcctgc acgagccggt cggcgtcgta gccggaatcg ccggccgctc
     6661 cgatgcagag gttcccgatg cccttcaaca ggctgtaccc attcaggacc agctcctggt
     6721 cggggagccc tcgctccgcg cgcgctgcct cgaggagcga cccgcagacg ggcacgaggc
     6781 ggtcgacgaa gtacgcgtgg agcgcgtcgg agcctgcgtt gtcgcctcgg agcgcgtctg
     6841 caaggccgtg cttggtcacc aggaagtcca cgaacagcgc cacccatgcc cgcaacgccg
     6901 cctctgccgt cgcgctctcg gtgagaagcc tcggccccgc ctccgcgcag gcctcgacct
     6961 ggtgccggta caccgcgacg accagcgccg gccgtgtcgg gaagtgccgg tagatggtgc
     7021 ccatcccaac tccggccttg gccgcgatct cgcgtacggg ggcgtccacg ccggactcca
     7081 cgaacaccgc cgcggcagca gcgagcaggt cctgctgact ccggcgcggg cgaccgaccg
     7141 gccggggtcc cgcctcgggc ggctgttcgt tcggtacatc ggtcatgggc tcgtcctcgt
     7201 gggtcgacgg tgagcttgct taacggaacg gcgttccgta aagttgcgga gcggcgctcc
     7261 gataccagta tcgcagagcg cgagcctcag aagggaaacg ccatgacctc agcaatcgtc
     7321 cacgctctgg acggcatcgt cggcgccggc accccggtcg tctccgctgc ccctgttacc
     7381 ctgtctgccc ccgaccgacc cgtcccgctg gaggtgcgcg tctccgcccc ggcgacggga
     7441 acgaatctcc ccgtcgtggt cttctcgcac ggcaacgggt ggaacctcga cggatactcg
     7501 cccctcaccg cgttctgggc ttcccgcggg ttcgtggtga tccagcccac gcacctggac
     7561 tcacgccgga acgggttcgg gttcgagcac ccggtgttcc cgaccatctg gaccgagcgg
     7621 atcgctgacc tcactcgcac cctggaccaa ctcgacacca tcgagggcgc cgtcccgggc
     7681 ctcgccggcc gcgtcgatcg cagtcgcgtc gccgccaccg gtcactcctg gggcggacag
     7741 accgcgcagt cgctgctggg cgcgcggatt ctcgacgaag agggcaacgt cggtgaggac
     7801 atgtccgacc atcgcgtcac cgctggcatc ctcttcgccg ccaccgggct cggtggcgac
     7861 gacctgcacc cgttcgcgaa ggccaacttc ccgttcatgc gcccctcgtt ccaggagctg
     7921 accacgccga ccctcgtggt cgccggcgac tgcgaccagt ccaagatgtc cagccgtgga
     7981 cccgactggt tcaccgacgc ctacacgcac agccccggag ccaccgacct gctcaccttc
     8041 tacggcgccg agcacgccct cggcgggatc gtcggatacg aggtcgccga gaccaccgac
     8101 gagaaccccg agcgcgtcgc cgtcatccag cgattgagca ccgcgtacct ccgcaccgcc
     8161 ctccacgtcg acgagaacag ctggggcgcc gctcgagctg cagtccgcga cggcgccgac
     8221 ccgatcggcc gcgtcgacag caagtgacac caggcccctc gccctcactc ctggcgatcc
     8281 ccacccagtt caccgcctcg atactcgtcg ccggtggcac ctccgaacat gcgtgctctg
     8341 gcaagccatc gccatcgcgc tctggaccac ggacgggacc atctcgtcct ggctcgcgct
     8401 ccaggtcgtg gtgctgatct gaggccgttc cgagcgacaa gcagaaaggg aaccacacat
     8461 gacgaatcc
//
LOCUS       DATDBZ010000132         3327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_252650, whole genome shotgun sequence.
ACCESSION   DATDBZ010000132 DATDBZ010000000
VERSION     DATDBZ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252650"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2198
                     /locus_tag="VIX62_03285"
     CDS             <1..2198
                     /locus_tag="VIX62_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY5859288.1"
                     /translation="DYDPLPLAQTSFLYAGDGSLITELHAAENRVVLRERQMSPFIRD
                     AAVAIEDRRFYMHHGVDMRAIARAAVVNANEGELVEGGSTITQQLVKNLYVGTADTLR
                     RKLDEATLAWQLEDRMSKDEILTKYLNTVYLGEGAYGVESAAQTYFSTDADELTLAES
                     ALLAGLIAAPNHFDPFVHPESAYGRRNVVLRSMREQRMIDRAQFRDAVREPILLRRGE
                     PATRYRFPYFVDYVKEWFLGNPAFGPSRQDRYKLLFTGGLRITTTLDPKTQGFAESAV
                     RSVLAYPTDPDGAMTVIDPRTGYVRAMVGGKDADYWRDSDAGRVNLATGKGGLGRQTG
                     SAFKPYALVTALEHGISPSAVYSAPASIDIPLQGGGVWHVTNAEGSGYGSMTLASATI
                     NSVNTVYAQLIQQLGADEVVETAQRMGMRCCPRVSEPRSPLSPYLSAVLGTNEANTLE
                     MASAYGTLATGGAHVDPVPVISVTDAQGETLWQAHPDPKQVLEPQVATVANGILEDAV
                     SYGTGRSAIIGRPQIGKTGTAETHTNAWFVGAVPQLTAAVWVGFHEGSIPMEPPRTRI
                     TVFGGTWPAQIWRLFMLKAAAGLPARTFPTPHVGYLSVSVDVSQSPYCLPNAYTLPQN
                     IDTLQFIEGTEPTEVCTTPTTVQSVPVPSVIGLDQASAALALEEAGFYVDVRVAISTQ
                     PPGTVIYQRPSAGTDAAQTSIVRITISEDAPTPSASPSAASPADQAGPG"
     gene            complement(2119..2757)
                     /locus_tag="VIX62_03290"
     CDS             complement(2119..2757)
                     /locus_tag="VIX62_03290"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY5859289.1"
                     /translation="MPKAFYGRSACEVAPDLLGRTLVRRMPDGQRLAGRIVETEAYEP
                     GDPASHGFRGITARNAAMFGPAGHLYVYFTYGNHWMLNAVTAEPSVGSAVLLRALEPL
                     EGLPVMAAARGRERSLDLCSGPGKLAQAMLVDGAFDGADLVRGSAIWIETGTPVPRDL
                     VGTSVRVGVRVGLNRAWRYFVTDDPFVSRGRPGPPGSRPRGSPTASAHPRRS"
     gene            complement(2769..>3327)
                     /locus_tag="VIX62_03295"
     CDS             complement(2769..>3327)
                     /locus_tag="VIX62_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5859290.1"
                     /translation="LAQRERVRALQGRVPSMRLLHGTELNIDPDGGVDWDDAFLEGFD
                     LTVASVHSHFGQSREQMTRRVIRAIENPRVHIIGHLTTRKIGRRDPVDLDLEAVFEAA
                     GRTGTALEINAYPDRLDLRDEHVLWARRHGVRFAIDTDAHAVGHLDMMRFGVATAQRG
                     WLTKDDVINAWPITKLQRFLRKGGS"
BASE COUNT          535 a         1206 c         1106 g          480 t
ORIGIN      
        1 atgactacga tccgctgccg ctcgcccaga cctcgttcct ctacgccggc gacggatcgc
       61 tcatcactga actgcacgcg gcggagaacc gcgtcgtgct ccgggagcgg cagatgtccc
      121 ccttcatccg cgacgccgcc gtcgcgatcg aggatcgccg gttctacatg caccatgggg
      181 tcgacatgcg cgcgatcgcg agggcggcag tcgtgaacgc gaacgagggc gagctcgtcg
      241 aaggcggatc gacgatcacc cagcagctcg tgaagaacct ctacgtcggc acggccgaca
      301 ccctgcgccg caagctcgac gaggccacgc tggcctggca gctcgaggac aggatgtcca
      361 aggacgagat cctcacgaag tacctcaaca cggtctacct cggcgaggga gcctacggcg
      421 tggagtcggc cgcccagacc tacttctcca ccgacgccga cgaactcacc ctcgccgagt
      481 ccgccttgct ggcgggcctc atcgcggcgc cgaaccactt cgacccgttc gtgcaccccg
      541 agagcgccta cgggcgtcgc aacgtcgtgc ttcgttcgat gcgcgagcag cgcatgatcg
      601 accgggcgca gttccgcgac gccgtccgtg agcccatcct gcttcgtcgc ggcgaaccgg
      661 ccacccggta ccggttcccg tacttcgtcg actacgtgaa ggagtggttc ctcgggaacc
      721 ccgccttcgg cccgagtcgc caggaccgct acaagctgct gttcaccggt ggccttcgca
      781 tcacgaccac gctcgacccg aagacccagg gcttcgccga gtcggcggtg cgctcggtgc
      841 tcgcgtatcc gaccgatccc gacggcgcga tgaccgtgat cgacccacgc accggctacg
      901 tgcgagcgat ggtgggcggc aaggacgccg actactggcg ggactcggac gccggccgcg
      961 tgaacctcgc caccggcaaa gggggtctgg gtcgccagac cggatcggcg ttcaagccct
     1021 atgcactcgt gaccgccctc gagcacggca tcagcccttc ggccgtgtac tcggcgccgg
     1081 cgtcgatcga catccccttg cagggtggcg gcgtgtggca cgtcacgaac gccgagggca
     1141 gcggctacgg ttcgatgacg ctcgcgagcg ccacgatcaa ctcggtgaac accgtctacg
     1201 cccaattgat ccagcagctc ggcgccgacg aggtcgtcga gaccgcccag cgcatgggca
     1261 tgcgatgctg cccgcgggtg agtgagccac gctcgccgtt gtcgccgtac ctgtccgcgg
     1321 tgctcggcac gaacgaggcc aacacgctcg agatggcgag cgcctacggc accctggcca
     1381 ccggtggcgc acacgtcgat ccggtgccgg tgatcagcgt gaccgacgca cagggcgaga
     1441 cactgtggca ggcacacccc gacccgaagc aggtgctcga accgcaggtc gcgacggtcg
     1501 cgaacggcat cttggaggat gccgtgagct acggcacggg gcgctcggcg atcatcggtc
     1561 ggccccagat cggcaagacc ggcacggcgg agacgcacac gaacgcatgg ttcgtcggcg
     1621 cggtcccgca gctgaccgcc gcggtctggg tcggattcca cgagggatcg atcccgatgg
     1681 aaccgccccg cacccgcatc acggtgttcg gcggcacgtg gcccgcgcag atctggcggc
     1741 tcttcatgct gaaggcggcg gccggcctgc cggctcgcac gttccccacg ccgcacgtgg
     1801 gctacctctc ggtctcggtg gacgtctcac agtcgcccta ctgcctgccc aacgcctaca
     1861 cgctgccgca gaacatcgac acgctgcagt tcatcgaagg caccgagccg accgaggtgt
     1921 gcaccacgcc gaccaccgta cagtcggtgc ccgtgccgtc ggtcatcggg ctggaccagg
     1981 catcggccgc gctcgccctc gaggaagccg gcttctacgt ggacgtgcgg gtggcgatct
     2041 cgacgcagcc gcctggcacc gtgatctacc aacgcccctc cgcgggcacc gacgcggccc
     2101 agacgagcat cgtgcggatc acgatctccg aggatgcgcc gacgccgtcg gcgagcccct
     2161 cggccgcgag cccggcggac caggccggcc ccggctgacg aacggatcgt cggtcacgaa
     2221 gtacctccac gcacggttga ggccgacccg cacgcccacg cggacggagg tccccacgag
     2281 atccctgggc accggtgtgc ccgtctcgat ccagatggcc gacccccgca cgaggtcggc
     2341 gccgtcgaag gcgccgtcga cgagcatggc ctgggcgagc ttgcccggcc ccgagcagag
     2401 gtcgagggaa cgctcgcgac cgcgtgcggc cgccatcacc ggcagcccct cgagcggctc
     2461 cagggcgcgc agcagcacgg cgctgccgac cgacggttcg gccgtgacgg cgttcagcat
     2521 ccagtggttc ccgtaggtga agtacacgta cagatgcccg gctggtccga acatcgccgc
     2581 gttcctcgcc gtgatgccgc ggaacccgtg gctcgccggg tcgcccggct cgtacgcctc
     2641 ggtctcgacg atgcgccccg cgagacgctg cccgtcaggc atccgtcgca cgagggttcg
     2701 cccgagcagg tccggggcca cctcgcacgc cgaacgcccg tagaacgcct tcggcagccg
     2761 gcggcggctc aggagccgcc cttacgcagg aatcgctgca gcttcgtgat cggccaggcg
     2821 ttgatcacgt cgtccttcgt cagccagccg cgctgcgcgg tcgccacgcc gaagcgcatc
     2881 atgtcgaggt ggccgacggc gtgcgcgtcg gtgtcgatcg cgaaccgcac accgtgtcgc
     2941 cgagcccaca acacgtgctc gtcccgcagg tcgagccggt ccgggtacgc gttgatctcg
     3001 agggcagtgc ccgtacgtcc ggccgcctcg aacacggctt ccagatcgag atcgacgggg
     3061 tcgcgccggc cgatcttgcg ggtcgtgagg tggccgatga tgtgcacccg ggggttctcg
     3121 atcgcacgga tcacccggcg ggtcatctgc tcgcgagact gcccgaaatg ggaatgcacc
     3181 gatgccacgg tgaggtcgaa cccctccagg aaggcatcgt cccagtcgac cccgccgtcg
     3241 gggtcgatgt tgagctcggt gccatggagc agtcgcatgc tcgggacccg gccctggagg
     3301 gcacgcaccc gctcgcgctg ggcgagc
//
LOCUS       DATDBZ010000133         2165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1684502, whole genome shotgun sequence.
ACCESSION   DATDBZ010000133 DATDBZ010000000
VERSION     DATDBZ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1684502"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VIX62_03300"
     CDS             complement(<1..880)
                     /locus_tag="VIX62_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY5859291.1"
                     /translation="MSQTSAPMTLAEKVWARHVVHRADGEPDILYVDLHLVHEVTSPQ
                     AFDGLRVHGRPVRRPDLTVATMDHNVPTTPGPVTDEISARQMQALATNAKDFGVTLFP
                     MGSPGQGIVHVIGPEQGYTQPGLVIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQT
                     LPQNRPGTMAVTVDGDLPADCSAKDIILAIINRIGTGGGVGHIIEYRGSAIRALSMEG
                     RMTICNMSIEGGARAGMVAPDDTTFAYVEGRPHAPSGADWEAAIQDWRTLPTDEGATF
                     DQEVTIDAASLRPYVSW"
     gene            1002..1787
                     /locus_tag="VIX62_03305"
     CDS             1002..1787
                     /locus_tag="VIX62_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY5859292.1"
                     /translation="MVSDGGGTIPDDDVHSAPPETGVGTLDRALDVLDAVEAGARSFT
                     DVVHATRLTRPTAHRMIRALEAHGLLMHVGGHGYALGTRLLRLAESASRNLPLRDLAH
                     PSLERLARATGESAQLYVRDGHARLCVDSVESDSELRTIVDIGASLPLTKGSAGKIFL
                     AWAGEHDVAHLLADLDPDPRDRLERQMATAKRRGWADSIGEREEGVASVSAPVFDQHG
                     MLTAVVSVSGPANRIGQLKGRHYAPAVTAAAAEIEAALHATRT"
     gene            1813..>2165
                     /locus_tag="VIX62_03310"
     CDS             1813..>2165
                     /locus_tag="VIX62_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859293.1"
                     /translation="MSHRAALLRPLEGWLSARRAITASVLSMCAVILIAATPGSPYHP
                     VLPESQGNGPLELLAKLLFLDRLPHGVLIALGFAAMISAGVTFLLMLWAAERGLISTR
                     TVVTLTITYHVAVLLL"
BASE COUNT          328 a          754 c          727 g          356 t
ORIGIN      
        1 cccagctgac gtacgggcgg agggatgccg catcgatcgt cacttcctgg tcgaacgtgg
       61 cgccttcatc ggtgggcagg gtgcgccaat cctggatcgc ggcttcccag tctgcccccg
      121 aaggcgcgtg ggggcgcccc tcgacgtagg cgaacgtggt gtcgtcgggc gcgaccatgc
      181 ccgcgcgggc gccaccctcg atcgacatgt tgcagatcgt catgcggccc tccatggaga
      241 gcgcacggat cgccgagccg cggtactcga tgatgtggcc gacgccgccg ccggtcccga
      301 tgcggttgat gatggccagg atgatgtcct tggcgctgca gtcggccggg aggtcgccgt
      361 cgaccgtgac cgccatggtg ccgggccggt tctggggaag cgtctgggtg gcgaggacgt
      421 gttcgacctc actggtgccg atgccgaacg cgagcgcgcc gaacgcgccg tgggtactcg
      481 tatgcgagtc cccacagacg atgaccagcc ccggctgcgt gtagccctgc tccggtccga
      541 tcacgtggac gatgccctga ccgggcgagc ccatggggaa cagcgtgacg ccgaagtcct
      601 tcgcgttggt ggcgagcgcc tgcatctgcc gcgcgctgat ctcatcggtg acggggccgg
      661 gcgtcgtggg cacgttgtgg tccatggtgg cgaccgtgag atcggggcga cggaccggac
      721 ggccatgcac gcggagccca tcgaacgcct gcgggctggt gacctcgtgc acgaggtgca
      781 ggtcgacgta gaggatgtcg ggttcgccgt ccgcgcgatg gacgacgtgg cgtgcccaga
      841 ccttctcggc caaggtcatc ggggctgacg tctgcgacat gcgatcctcc ggtagcgccg
      901 ggggcgaacc cggcctacat cccgtgcgtg gactggtacc tttgttccac tatttggaac
      961 tttgttccac tatttggaac agatgccagg gaggatagca ggtggtttcg gacggcggcg
     1021 gcacgatccc cgacgacgac gtccactccg cgccgcccga aacaggtgtg ggcacgctcg
     1081 atcgcgcact cgacgtgctc gacgccgtcg aagccggggc tcgcagcttc accgacgtgg
     1141 tgcacgccac ccgcctcacg cgccccaccg cccaccgcat gatccgagcc ctcgaggcgc
     1201 acggcctgct gatgcacgtc ggcgggcatg gctacgcact cggcacccgg ctgctgcgcc
     1261 tcgcagagag cgcctcgagg aacctcccgc ttcgggacct ggcgcaccca tccctcgaac
     1321 ggctcgcgcg ggcaaccggc gaaagtgcgc agttgtacgt gcgcgacggt cacgcccgtc
     1381 tgtgcgtcga ctccgtggaa tcggacagcg aactccgcac gatcgtcgac atcggcgcct
     1441 cgttgccgct cacgaaaggg tcggccggca agatcttcct cgcatgggcc ggggagcatg
     1501 acgtcgcgca cctcctcgcc gacctggacc ccgaccctcg cgaccgcctc gaacgccaga
     1561 tggccaccgc gaaacgacgg ggctgggctg attccatcgg cgaacgggag gagggcgttg
     1621 cgtcggtcag cgcacccgtg ttcgaccaac acggcatgct gaccgcggtg gtgtcggtct
     1681 cgggtccggc gaacaggatc ggacagctca agggacgcca ctacgcgccc gccgtgacgg
     1741 ccgcggccgc cgagatcgag gccgccttgc acgcgacgcg gacctagcac gattcccgta
     1801 agctcgaccg acatgtcgca ccgcgctgcc ctgctccgac cgctcgaagg atggctctcc
     1861 gcgcgccgcg cgatcaccgc atcggtcctc agcatgtgcg cggtgatcct gatcgcggcc
     1921 accccgggtt cgccatatca ccccgtgctg cccgagagtc agggcaacgg gcccctcgaa
     1981 ctcctcgcga aactgctctt cctcgatcgt ctcccccacg gcgtactcat cgcgctggga
     2041 ttcgcggcca tgatctcggc gggggtcacc ttcctgctca tgctctgggc tgcggaacgt
     2101 ggcctcatct cgacccgaac cgtcgtgacc ctgacgatca cctaccacgt cgcggtcctg
     2161 ttgct
//
LOCUS       DATDBZ010000134         3019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_865347, whole genome shotgun sequence.
ACCESSION   DATDBZ010000134 DATDBZ010000000
VERSION     DATDBZ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865347"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VIX62_03315"
     CDS             complement(<1..769)
                     /locus_tag="VIX62_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5859294.1"
                     /translation="MTEITMAGALNAALRDALRDDPRVLVFGEDVGRSGGVFRVTDGL
                     QTEFGAERVFDTPIAEAGIAGAAVGLAFAGWRSVVEMQFDAFSYPALEQIVDHIAKMR
                     ERTRGRVDMPIVIRMPAFGGIKGKEHHGESPETFYVHTAGLKVVAPSGALDAYRLLRL
                     AIADPDPVIVLEPKARYWSKEDGDLAVEGPGIGDGRVLRDGGACTVFSYGAMVARCLE
                     AAETVAGEGIETRMVDLRSLSPLDVDLIAGCVRDTGRA"
     gene            complement(762..1826)
                     /locus_tag="VIX62_03320"
     CDS             complement(762..1826)
                     /locus_tag="VIX62_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY5859295.1"
                     /translation="MTQIPEHWRGTEPLQVLAPDGTPVGDTDVGLDVTALRAILHWML
                     LARRLDRECIALQRQGELTVYPGFEGQEAAQIGSAMALGPDDFVFPTFRELAVALVRG
                     VDPVQYLAYHRGTWHGGPYDPRATRFGPICIPVATQIVHGVGYAMGLKLDGARACSIA
                     YFGDGSASEGDFHEGANLAGVFTAPVILFCQNNGWAISMPTANQTAGEIWKRADGYGF
                     PGFRVDGNDVLAVYRATKQAAERAYAGDGPTLIEALTYRIGAHSTADDPGRYRDDAEV
                     EAARAFDPITRFRTWLVAQGIVDEDTIGSWGEEIEAQVLAIREGVITQPAPPVEWMFD
                     WTYADPPATLARQRREVVGD"
     gene            1864..2697
                     /locus_tag="VIX62_03325"
     CDS             1864..2697
                     /locus_tag="VIX62_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859296.1"
                     /translation="MAAGGKLGAMVPSAFLRVFQPLDAFEREEQLHWERYLLDGPAAQ
                     SLRPRYADHRTVGSLGLLAPFGAEHAEVRVVDGRTYVSPMRLRLRVLAAMLSFREAQP
                     MELWEQFVPKKEARRAARDLARFRRHDPSAVPFCHQSPWHVPIRWFVLFRDEERRLTT
                     DEAGGTHLHYRTTTRRAIRRAEQAVVPLRRSDLGPISDLIVDLHQWMHAFDPSSLLEL
                     DYGGLSALLTWDELDEDHSARDVHEALEALEREEFPRAADVYQGVLSRWAEVGSREAM
                     N"
     gene            complement(2724..2799)
                     /locus_tag="VIX62_03330"
     tRNA            complement(2724..2799)
                     /locus_tag="VIX62_03330"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2764..2766),aa:Phe,seq:gaa)
BASE COUNT          455 a         1058 c         1042 g          464 t
ORIGIN      
        1 cggcgcgacc ggtgtcacgc acgcagccgg cgatcagatc cacgtcgagc ggcgagagcg
       61 atcgcaggtc gaccattcgc gtctcgatgc cctcgcccgc caccgtctcc gcggcttcga
      121 ggcagcgtgc caccatcgcg ccgtacgaga acaccgtgca ggccccgccg tcccggagca
      181 cgcgcccatc gccgatcccg ggaccctcca cggcgaggtc cccgtcctcc ttcgaccagt
      241 agcgggcctt gggttcgagg acgatcacgg ggtcgggatc ggcgatcgcg aggcgcaaca
      301 agcggtaggc gtccagcgca ccggagggcg ccaccacctt cagtcccgcc gtgtgcacgt
      361 agaacgtctc ggggctctcg ccgtggtgct ccttgccctt gatgccgccg aacgcgggca
      421 tccggatcac gatcggcatg tccacgcgcc cgcgcgtgcg ctcgcgcatc ttggcgatgt
      481 gatcgacgat ctgctcgagg gccggatagc tgaacgcgtc gaactgcatc tcgacgacgc
      541 tgcgccatcc ggcgaacgcc agacccaccg cggcacccgc gatccccgct tcagcgatcg
      601 gtgtgtcgaa cacccgctcc gccccgaact ccgtctgcag gccgtcggtg acgcggaaca
      661 ccccgccgga gcggccgacg tcctcaccga agaccagaac ccgcggatca tcgcggagcg
      721 cgtcgcgcag cgccgcgttg agggcgccgg ccatcgtgat ctcagtcacc gaccacctcg
      781 cggcgctggc gagccaaggt ggcgggcgga tccgcgtatg tccagtcgaa catccactcc
      841 accggcggcg ccggctgggt gatcacgcct tcgcggatgg ccagcacctg cgcctcgatc
      901 tcctcgcccc acgagccgat cgtgtcctcg tccacgatgc cttgggcgac cagccacgtg
      961 cggaaccgcg tgatcggatc gaacgctcgc gcggcctcca cctcggcatc gtcgcggtag
     1021 cgcccggggt cgtcggccgt ggagtgtgcg ccgatccggt aggtcagcgc ttcgatcagg
     1081 gtcgggccgt cgccggcgta cgcgcgctcg gcggcctgct tggtggctcg gtacaccgcg
     1141 agcacgtcgt tgccgtccac ccggaagccc gggaacccgt agccatcggc gcgcttccag
     1201 atctccccgg cggtctggtt cgcggtaggc atgctgatcg cccagccgtt gttctgacag
     1261 aacaggatca cgggggcggt gaacacgccc gcgaggttgg cgccctcgtg gaagtcgccc
     1321 tcgctggcgc tgccgtcgcc gaagtaggcg atcgaacagg cgcgcgcgcc gtcgagcttc
     1381 aagcccatcg cgtagcccac gccgtgcacg atctgggtgg cgacggggat gcagatcggt
     1441 ccgaaccgcg tggcccgggg gtcgtacggg ccgccgtgcc acgttcctcg gtggtaggcg
     1501 aggtactgca cggggtcgac gcctcgaacc agggccaccg cgagttcgcg gaaggtcggg
     1561 aacacgaagt cgtcgggtcc cagcgccatc gcgctgccga tctgcgcggc ctcctgccct
     1621 tcgaacccag gatagaccgt gagctcgccc tgacgctgga gggcgataca ttcgcggtcc
     1681 agccgccggg ccagcagcat ccagtgcagg atcgcgcgca gggcggtcac gtcgaggccg
     1741 acatcggtat cgcctacggg cgttccgtcg ggggccagga cctgcagggg ctcggtgcca
     1801 cgccagtgtt cgggtatctg ggtcacttca gggccccctc gtcggacgcc gagagcatag
     1861 gggatggcgg ccggcggtaa gctcggggcg atggtgccca gcgcgttcct cagggtgttc
     1921 cagccgctcg atgcgttcga gcgcgaggag cagctccatt gggagcgtta cctgctcgat
     1981 ggtcccgccg cgcagtcgct ccgcccgcgc tacgccgatc atcgaacggt ggggtcgctg
     2041 gggctgttgg cgcccttcgg ggccgagcac gccgaggtcc gcgtggtgga cggccgcacg
     2101 tacgtgagcc ccatgcgtct gcgtctgcgc gtcctggccg cgatgctgtc gttccgcgag
     2161 gcccagccga tggagctgtg ggagcagttc gtgcccaaga aggaggcccg ccgggccgcc
     2221 agagacctgg ctcgcttccg tcggcacgac ccctccgccg tgccgttctg ccatcagagt
     2281 ccgtggcacg tgccgatccg gtggttcgtg ctgttccgcg acgaggagcg tcggctcacc
     2341 accgacgagg cgggaggcac ccacctgcac taccgcacca cgacgagacg ggcgatcagg
     2401 cgcgccgagc aggcggtcgt gccgctgcgc cggtccgacc tggggccgat cagcgatctc
     2461 atcgtcgacc tgcaccagtg gatgcacgcg ttcgatccgt cgtcgctgtt ggaactcgac
     2521 tacggcggcc tgtcggcgtt gctcacgtgg gacgagctgg acgaagacca cagcgcccgc
     2581 gacgtgcacg aggcgctcga ggcgctggaa cgcgaagagt tcccacgagc cgccgacgtc
     2641 taccagggcg tgttgagtcg atgggccgag gtgggcagcc gcgaagcgat gaactgaccg
     2701 cgtcgtgatc gcggttcctc cgatggtggc caggggcaga atcgaactgc cgacaccgcg
     2761 gttttcagcc gcgtgctcta cctactgagc tacctggcca agccgagccg gtcgaagatc
     2821 tggcagaaag caccaccagg ctcggttccg gatgtccaga gtctagcgac tcgtcgaccg
     2881 agtcgggtcc ggcagcgccg atttcagacg ggtttctgac cggtggcgcg gccccacgcg
     2941 aacgcggcga aggcgtcggg ggactcggac catgcgagcc aagcgcgctt caactcatcg
     3001 atctccgcaa ggtcggcca
//
LOCUS       DATDBZ010000135         3396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_454445, whole genome shotgun sequence.
ACCESSION   DATDBZ010000135 DATDBZ010000000
VERSION     DATDBZ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454445"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VIX62_03335"
     CDS             complement(<1..766)
                     /locus_tag="VIX62_03335"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY5859297.1"
                     /translation="MPQRDHNPSGRRLHLPIVGVTNGPGVDPEQAGVNARGERKPPWL
                     KVRLQTGPNYGELRDIMRGRELHTVCEEAMCPNIHECWEDREATFLILGSKCTRRCGF
                     CDVMTAKPDPVDEGEPERIADAVRAMGLRYVVLTGVARDDLPDGGARIWAASIRAIRR
                     AVPGCRVEILPSDFKGGETDIATVLDAAPDVFAHNLETVRRLHDRIRPAFGYDRSLDV
                     LRIAKRLHPDMVTKSNLILGMGERPDEIEPAMHDLVE"
     gene            885..2120
                     /locus_tag="VIX62_03340"
     CDS             885..2120
                     /locus_tag="VIX62_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY5859298.1"
                     /translation="MSDQSPSEEREPTLVKVQLPSWRWIFMLLGSIAAVGLASLVFLA
                     FFRQVRDLLIWVVVALFASFALEPAVDWLAKRGWRRGVATGVILFGLAVFAVVMVALM
                     IPLVVTQLRELIDAAPDILRTISGYTTRWFNVDVSPEALQQQLSSADSELGQFATNVA
                     GNLFGFATSILGTVFKLLTIGLFTFYLTADGPRFRRAICSVLPPKRQEAVLWTWEVAI
                     EKTGAYLYSRLLLAVVSGVATFVVLTALGVPFAVPLAVWMGLVSQFIPTIGTYIAMAL
                     PLVVAVVQSPVDALILLIFFVLYQQLENYFLSPRITARTMEMHPAIAFGCAIAGASIA
                     GIVGAFLALPIAAIFQAIGSTVVERHAVVESDLTRDAEDAQEAFEQERARDGGRPPKV
                     MDRLRARMNKDDDEPDSET"
     gene            complement(2117..>3396)
                     /locus_tag="VIX62_03345"
     CDS             complement(2117..>3396)
                     /locus_tag="VIX62_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY5859299.1"
                     /translation="AVMILDADGTARYSQGILLDVTERRLAEQQVREAEERYRAIVEH
                     VPAAIYLDLADRSMRTVYISPQIEAITGISPQEWMDDPEAWLKIVPEDRDQVERDYLR
                     AIADGTAWNAEYRMRTRDGRTIWVHDETTFLHDEDGNPTYLQGVLMDITERKLAEQAL
                     RESEQREREAAERLRALDEMKNTFLAAVSHELRSPLTSILGLSITLERTPDMDAEDRI
                     DLQERVSSNARKLDRLLKDLLDIDRLNRGIVEPQYRTADVGALTRRAVESLDALAERT
                     VVIDVGAVVLRVDPPKLERIVENLAMNAARHTASDRTIWVRLEPHDHGALLTVEDDGT
                     GVDEHLREAIFEPFRQGPSVSSHSPGTGIGLSLVGRFAQLHGGRAWVEDRPGGGASFK
                     VFLPAGPEGTAAASAEHPTAPSATHDGDTAAAG"
BASE COUNT          452 a         1116 c         1207 g          621 t
ORIGIN      
        1 cttccacgag gtcgtgcatc gcgggttcga tctcgtcggg tcgttcgccc atccccagga
       61 tgaggttgga cttcgtgacc atgtccggat gcaggcgctt ggcgatccgc aacacgtcga
      121 gggaacggtc gtagccgaac gccggacgga tccggtcgtg caaccggcgg acggtctcca
      181 ggttgtgcgc gaagacgtcg ggcgcggcgt cgagcacggt ggcgatgtcg gtctcgccac
      241 ctttgaaatc gctcggcagg atctcgacgc ggcagccggg gaccgcgcgc cggatcgcgc
      301 ggatgctcgc tgcccagatc cgcgccccgc cgtccgggag gtcgtcacga gcgacgccgg
      361 tcagcacgac gtagcgcagg cccatggcgc ggaccgcgtc agcgatgcgc tcgggctcgc
      421 cttcgtcgac cgggtcgggc ttggcggtca tcacgtcgca gaagccgcag cgtctggtgc
      481 acttcgagcc caggatcagg aacgtggcct cccggtcttc ccagcactcg tggatgttgg
      541 ggcacatcgc ttcttcgcac acggtgtgga gctcgcgccc gcgcatgatg tctcggagct
      601 ccccgtagtt tggcccggtc tggagccgga ccttcaacca cggcggcttc cgttcacccc
      661 tggcgttcac gccggcctgc tcggggtcga cgccggggcc gttcgtgacg ccgacgatcg
      721 gcagatggag ccgccggccg gaggggttgt ggtcgcgctg gggcatggac ctcgagtcta
      781 gccgcggacc cgcggagcgc ccgtggctgc gcgggagctt ccggttgccg gggtatcctc
      841 gcgccgtcgg gcgagccgcg cccgacggag gtccgaggga gccgatgagc gaccagtctc
      901 cgagcgagga acgcgaaccc acgctcgtca aggtgcagct gccctcgtgg cgctggatct
      961 tcatgctgct gggctcgatc gccgcggtgg gcttggcgtc gctggtgttc ctcgcgttct
     1021 tccgccaggt cagggatctg ctgatctggg tcgtcgtggc gctgttcgcc tcgttcgcgc
     1081 tggaaccggc ggtggactgg ctggccaagc ggggatggcg tcgcggggtg gccacggggg
     1141 tgatcctgtt cggcctcgcg gtcttcgccg tggtgatggt ggccctgatg atcccgttgg
     1201 tggtgaccca gctccgtgag ctgatcgacg ccgcgccgga catcctgcga acgattagtg
     1261 ggtacaccac gaggtggttc aacgtcgatg tctcgcccga ggcgctgcag cagcagctct
     1321 cgtcggccga ttccgagctg gggcagttcg ccacgaacgt cgcgggcaac ctgttcggct
     1381 tcgcgacctc gatcctgggc acggtgttca agctcctgac gatcgggctg ttcaccttct
     1441 acctcaccgc cgatggcccc cggttccgcc gggcgatctg ttcggtgctg ccgccgaaac
     1501 ggcaagaggc cgtcctgtgg acctgggagg tggcgatcga gaagacgggc gcctacctct
     1561 attcacggtt gctgctggcg gtggtctccg gggtggcgac cttcgtggtg ttgaccgcgc
     1621 tcggggtgcc gttcgcggtg ccgctcgccg tctggatggg gctggtgtcc cagttcatcc
     1681 cgacgatcgg cacgtacatc gcgatggcgc tgccgctggt cgtggccgtg gtgcagagcc
     1741 cggtcgatgc gctgatcctg ctgatcttct tcgtgctgta ccagcagctc gagaactact
     1801 tcctgagccc tcgcatcacc gcgcggacca tggagatgca cccggcgatc gccttcgggt
     1861 gcgccatcgc gggcgcgagc atcgccggca tcgtgggggc gttcctcgcg ctgccgatcg
     1921 ctgccatctt ccaggccatc ggctccaccg tcgtcgagcg tcacgccgtg gtcgagagcg
     1981 atctcacgcg cgatgccgag gatgcacagg aagcgttcga gcaggagcgc gcccgtgacg
     2041 gcgggcgacc cccgaaggtg atggaccggc tccgcgcacg gatgaacaag gacgacgacg
     2101 aacccgactc cgagacctag cccgccgcgg cggtgtcgcc gtcgtgggtc gcggaggggg
     2161 cggttgggtg ctcggccgat gccgcggcgg ttccctcggg tccggccggc aggaacacct
     2221 tgaaggaggc gccgccgccg ggtcgatcct ccacccacgc tcggccaccg tggagctgcg
     2281 cgaatcggcc caccaacgac aggccgatcc cggtgccggg cgagtgggag gacaccgagg
     2341 gcccctggcg gaacggttcg aagatcgcct cgcggaggtg ttcatcgacg cccgtcccgt
     2401 catcctcgac ggtgagcagg gcgccgtggt catggggttc gagtcgaacc cagatcgtgc
     2461 ggtcgctcgc cgtgtgccgt gcggcgttca tcgcgaggtt ctcgacgatg cgctcgagct
     2521 tcggcggatc cacccggagc accacggcac cgacgtcgat caccacggtg cgttcggcga
     2581 gcgcatccaa gctctcgacc gcgcgccggg tcagggcgcc gacgtcggcg gtgcggtact
     2641 gcggttccac gatgcctcgg ttgagccggt cgatgtcgag gaggtccttc agcagacggt
     2701 ccagtttgcg ggcgttcgat gagacccgtt cctgcaggtc gatccggtcc tcggcgtcca
     2761 tgtccggcgt gcgttccagc gtgatcgaga gtcccaggat cgaggtgagc gggctccgga
     2821 gctcatgcga gacggcggcg aggaacgtgt tcttcatctc gtcgagggct cgaagtcgtt
     2881 cggccgcttc ccgttcacgc tgttcggatt cgcgcagcgc ctgctcggcg agcttgcgct
     2941 cggtgatgtc catcaagacg ccctggaggt aggtcggatt gccgtcctca tcgtgcagga
     3001 acgtcgtctc gtcgtggacc cagatcgtgc gaccgtcgcg cgtgcgcatc cggtactcgg
     3061 cgttccatgc cgtgccatcg gcgatggctc gcaggtagtc gcgctccacc tggtcgcggt
     3121 cctcgggcac gatcttgagc caggcctccg gatcgtccat ccactcctgc ggcgagatcc
     3181 cggtgatggc ttcgatctgc gggctgatgt acaccgtgcg catcgagcgg tccgccaggt
     3241 cgaggtagat cgcggccggc acgtgttcga cgatggcgcg atagcgctcc tcggcctccc
     3301 gcacctgttg ctcggcgaga cgccgttcgg tgacgtcgag caggatgccc tggctgtatc
     3361 gagccgtgcc gtcggcgtcc aggatcatca ccgcct
//
LOCUS       DATDBZ010000136         6079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1887117, whole genome shotgun sequence.
ACCESSION   DATDBZ010000136 DATDBZ010000000
VERSION     DATDBZ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887117"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VIX62_03350"
     CDS             <1..498
                     /locus_tag="VIX62_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859300.1"
                     /translation="PYWLMGTIFAIMGHGMGATMAPMTAAVMNSVGPQRAGLGSAMTN
                     TSREVGGVFGIALLGTILFTRLKSVLVPSLAGLGLTAQQQASIATSASHGQLDVTQYG
                     LTAQQQAAVGQAFGDAFMSGFRVALLVAGAVLLVAALLANRFIPGREAVREAAPADVP
                     VAVEA"
     gene            580..1695
                     /locus_tag="VIX62_03355"
     CDS             580..1695
                     /locus_tag="VIX62_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859301.1"
                     /translation="MEMALRVISVVGGTFLVLLAVSSAIRTVILPRAAPSLVTRGVFR
                     SLGVVFRSLASGDYARRDRVMSLYAPLGLVCTLAAWLLLTFAGFGFIFWGLEGAVGVS
                     EALELSGSSLFTLGFLRPETFWLLMVSYIEAAIGLFLLALLITYLPSMYGAFSRREVA
                     VTALSVRAGEPPSGREMIWRYWRLERMDHLREVWREWERWFVDVEETHTSLPALVFFR
                     SPLPDHSWATAAGAVLDGAALTLSSVQVPREVEAEFCIRAGYLCLRRVADYFRIPHDP
                     DPTPDTPISITRAEWEEAVAFLESEGVPIKPDREQAWRDFAGWRVNYDTVLLALANLT
                     IAPEAPWSSDRSQDQGFRLRRMGGIKPVKHPPTSGRA"
     gene            complement(1676..2158)
                     /locus_tag="VIX62_03360"
     CDS             complement(1676..2158)
                     /locus_tag="VIX62_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY5859302.1"
                     /translation="MELLAFNKDLYLGIVFIHIFAAIIWVGGAFIFQLRIAQLRKAND
                     TAGFLQLGQDAERTGQRVFMPASIVVLLAGIAMVWYGPYSFKPWIALALVGIVITSLV
                     GALYLGPQGGKFARLAQERGVEDPDVVAARDRLVLVSRIDYVVLVLIVLDMVFKPGLT
                     "
     gene            complement(2256..3554)
                     /locus_tag="VIX62_03365"
     CDS             complement(2256..3554)
                     /locus_tag="VIX62_03365"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859303.1"
                     /translation="MADHLRPDPEPEVTRGLVARVREHLVDVTPLRMSADFRRLFIGR
                     SISEFGDEIVAVAVPFMVYEITKSTLAVGLLGLCELVPVFVFPIVGGAAADAMERRRL
                     MIVSQVFLALMSLALAVNLTLDEPKLWPFYVFATLSAGMYTFNRPSLSTWPARLLPAA
                     LLPSSNALEAGFGTAVGMLGPVVAGVLLVVISPAGVFAFDAFTFLVAIAFVWRMAPSP
                     PHEDAPALGWDAIADGFRFLKGKRVVQSVFLADLNAMIFAFPVALFPAVADGFAKGGD
                     SAAILGLLTAAPAVGAFLATAFSGRAKHVRRQGRAILVAIAIWGAAIVVFGLSNVLWL
                     SLLMLAIAGMGDMVSGIFRATILQTAVEDRYRGRLEGIGMAVWATGPSIGNVESGAVA
                     TVWNVQGSIVLGGVLTIAGIGVLRWFAPGFWRYDSAHPTS"
     gene            3630..4274
                     /locus_tag="VIX62_03370"
     CDS             3630..4274
                     /locus_tag="VIX62_03370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5859304.1"
                     /translation="MADGDPSRDDDFSAVNRATYDRISRQYADRQERQKPADGRWFPV
                     LEDEFLASVTAHGVIADLGCGPGLDGSRFIRAGFRAVGMDLSASMLAIAARSLGGSVA
                     QADLRALPIRSSSLDGIWCCAALLHVPEDDTSGVLHGFRRALKPDGTLALVTALGDGH
                     RLEPVPYAPGERRWFAYRHADRLGQQLRETGFRVRVDDVVASNREWITVLAEAV"
     gene            complement(4285..5112)
                     /locus_tag="VIX62_03375"
     CDS             complement(4285..5112)
                     /locus_tag="VIX62_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY5859305.1"
                     /translation="MPEIRALSERLDQIMTGGTLAKLDQLQFSSLKTFAPRPDEALGH
                     TIESVRSLGKYVVIQLDGGMRLLVHLSQGGRVDVETPPKQTKPRGAVLRIRVTDRPSV
                     LVKEFGKERKAGWWVLAPGDDGPLDKLGPEVLTDAADALLRESDDGRRVHTILRDQRT
                     IAGVGRGYSDDILHCAKLSPYAVLAKLTPEERERLIAATHSILDEALEEERRRNGGLP
                     TKLGDHFVVHNRAGEPCPVCGDDLRRVSYESHEVVYCPTCQTGGKVLADRRMSRLLR"
     gene            complement(5134..>6079)
                     /locus_tag="VIX62_03380"
     CDS             complement(5134..>6079)
                     /locus_tag="VIX62_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5859306.1"
                     /translation="SAMVTARHAKLGEDFTDGVYYVSEQAHASVPKAATIAGFSKRNV
                     RIVATDDELRMDPQALRTQIALDRADGLRPFLVAPSVGTTNTGAIDPIADIADIAEAE
                     DLWLHVDGAYGGFFQLTDRGRERFRGTERSDSVTLDPHKGLFLPYGTGGLVVRDGAAM
                     RDAHYEGAAYLQDLPPTGELPNYSEHSAELSRDWRGLRVWMPLALHGVSAFREALDEK
                     LDLAGYLADAFRDDDRIELCWEPQLTVIPFRLAGADDDTQREFLARINASKRVFLSST
                     MIGGRYVLRVCIVSHRTHKDRIDECIEIVGRAADELRA"
BASE COUNT          950 a         2129 c         2056 g          944 t
ORIGIN      
        1 ccctattggc tcatgggaac gatcttcgcc atcatgggcc atggcatggg cgccacgatg
       61 gcacccatga ccgcggcggt catgaactcg gtgggaccgc agcgtgccgg actcggttcg
      121 gccatgacca acacgagtcg cgaggtaggc ggggtgttcg gcatcgcgct gctcggcacg
      181 atcctgttca cccggctcaa gagcgtcctc gttcccagcc tcgcgggcct cggcctcacg
      241 gcccagcagc aggcgagcat cgcgacctcg gcgagccacg gccaactcga cgtcacgcag
      301 tacggcctca ccgcgcagca acaggcggcc gtcggccagg ccttcggcga tgccttcatg
      361 agcgggttcc gggtggcgtt gctggttgca ggggcggtgc tcctggtggc ggcgctcctc
      421 gccaaccggt tcatccccgg tcgggaggcg gtgcgggagg ccgcacccgc cgacgtgccg
      481 gtcgcggtcg aggcctagcc gaagcctccg ccggtccctc ccccgccgcc accgccgctc
      541 gagagccttc tgctccgacg tctgcgaata tccctggtca tggagatggc gctgcgggtg
      601 atctcggtgg tcggcgggac cttcctcgtg ctgctggccg tctcctcggc gatccgcacc
      661 gtgatcctgc cccgcgcggc tccctcgttg gtgacgcgag gggtcttccg cagcctcggg
      721 gtggtgttcc ggtcgttggc ctcaggcgac tacgcgcgcc gcgaccgggt catgtcgctg
      781 tatgccccgc tcggcctcgt gtgcacgctc gccgcgtggc tcctgctcac cttcgcgggg
      841 ttcgggttca tcttctgggg cctcgagggc gcggtggggg tcagcgaggc gctcgagctc
      901 tccggatcct cgctgttcac gctcggcttc ctgcggccgg agacgttctg gctgttgatg
      961 gtctcctaca tcgaggctgc gatcggcttg ttcctgctgg cgctgctgat cacgtacctg
     1021 cccagcatgt acggcgcttt cagccggcgc gaggtcgcgg tgaccgcgct gtcggtgcgc
     1081 gccggcgagc cgccctcagg acgcgagatg atctggcgct actggcggct ggagcgcatg
     1141 gaccacctgc gtgaggtgtg gcgtgaatgg gagcgctggt tcgtcgacgt ggaggagacg
     1201 cacacctcct tgccggcgct ggtgttcttc cgatcgccgc tgcccgacca ttcctgggcc
     1261 accgccgccg gtgccgtgct cgacggtgcc gccctgacgt tgtcgagcgt gcaggtaccg
     1321 agagaggtgg aggcggagtt ctgcatccgg gcgggctacc tgtgcctgcg cagggtggcc
     1381 gactacttcc gcatcccgca cgatcccgat cccacgcccg acacgccgat ctcgatcacg
     1441 cgagcggagt gggaagaggc cgtggcgttc ctcgagagcg aaggggtgcc gatcaagccc
     1501 gaccgcgagc aggcatggcg cgacttcgcc gggtggcgcg tcaactacga cacggtgctc
     1561 ctggccttgg cgaacctcac gatcgcgccg gaagcgccgt ggagctcgga tcggtctcag
     1621 gatcaggggt tccgactacg gcggatgggt gggatcaagc ccgtgaagca cccacctacg
     1681 tcaggccggg cttgaacacc atgtcgagga cgatcagcac cagcaccacg tagtcgatgc
     1741 gcgagaccag gacgagccgg tcgcgagcgg cgaccacgtc cgggtcctcg acgccgcgtt
     1801 cctgcgcgag ccgcgcgaac ttcccccctt gcggccccag gtagagcgca ccgaccagcg
     1861 atgtgatcac gatcccgacg agcgccagcg cgatccacgg cttgaacgag tacgggccgt
     1921 accagaccat cgcgatcccc gcgagcagca cgacgatcga ggcgggcatg aacacccgct
     1981 ggccggtccg ctcggcgtcc tggcccagct gcaggaaccc cgcggtgtcg ttcgccttcc
     2041 gcagttgagc gatcctgagc tggaagatga acgcgccgcc gacccagatg atggcggcga
     2101 agatgtggat gaacacgatc cccaggtaga ggtccttgtt gaacgccagc aactccatga
     2161 acgtcctccc aatcagtgct cggcccacga aggccgccgc ccccgggtca ggcggacgat
     2221 accgcggccc gaggggtgca cggaagcacc cgggctcacg atgtgggatg ggccgagtcg
     2281 tagcgccaga agcccggggc gaaccaccgc agcaccccga tcccagcgat cgtgaggacg
     2341 ccgcccaaca cgatggagcc ctggacgttc cacacggtgg ccacggcgcc cgactcgaca
     2401 ttgccgatcg agggcccggt ggcccagacc gccatgccga tgccttccag gcggccgcgg
     2461 tatcgatcct cgaccgcggt ctgcaggatc gtggcccgga agatgccgct caccatgtca
     2521 cccatccccg cgatcgcgag catcaacagc gacaaccaca ggacgttcga taggccgaag
     2581 accacgatgg ctgcgcccca tatcgcgatc gccaccagga tcgcccgtcc ctgccggcgc
     2641 acgtgcttcg cacgcccgga gaacgccgtc gccaggaagg ctcccacggc gggggcggcc
     2701 gtgagcaagc ccaggatcgc cgcgctatca ccgcccttcg cgaacccatc ggccaccgca
     2761 gggaacaacg cgacggggaa agcaaagatc atcgcgttga ggtcggccag gaataccgac
     2821 tgcaccaccc tcttgccctt caggaaccgg aacccgtccg cgatcgcgtc ccaccccagg
     2881 gcaggcgcgt cctcatgcgg cggcgatggc gccatgcgcc atacgaacgc gatcgccacc
     2941 aggaacgtga acgcgtcgaa ggcgaacacc cccgccggcg agatgaccac caacaggacg
     3001 ccggccacca ccggacccaa catgccaacc gcggtgccga aacccgcctc gagcgcattg
     3061 gacgagggca gcagcgcggc gggcaggagc cgcgcgggcc acgtcgagag cgacggccgg
     3121 ttgaacgtgt acatgcccgc cgacagcgtg gcgaacacgt agaacggcca gagcttcggt
     3181 tcatccaggg tgaggttcac ggcgagggcc agcgacatca gcgccaggaa cacctgcgag
     3241 acgatcatca gccgtcgtcg ctccatggcg tcggccgccg ccccgcccac gatcgggaac
     3301 acgaacaccg ggaccagttc gcacaacccg aggagtccga ccgccagggt ggacttcgtg
     3361 atctcgtaca ccatgaacgg caccgccacc gcgacgatct cgtccccgaa ctccgagatc
     3421 gatctgccga tgaacaacct gcggaagtcg gccgacatgc ggagcggcgt gacgtcgacc
     3481 aggtgctcgc gaacgcgcgc caccaacccg cgtgtgacct cgggctcggg atcgggtcgc
     3541 aggtgatcgg ccatgacggc cgaccacgct agcgcagcgt ccaaggcaga gcgctccgat
     3601 ccgcgctgac ttcctgcaac gatgtacagg tggccgacgg cgacccatct cgagacgacg
     3661 acttctccgc cgtgaatcgc gccacgtacg accggatctc tcgccagtat gccgatcggc
     3721 aggagcgaca gaaaccggct gatgggcgct ggttccccgt gctcgaggac gagttcctcg
     3781 cgagcgtgac cgcgcatggc gtgatcgcgg acctgggctg cggcccgggc ctggacgggt
     3841 cccggttcat ccgggccggc ttccgcgcgg tcggcatgga cctgtcggct tcgatgctgg
     3901 cgatcgctgc ccggtcgctt ggcggctcag tggcccaggc ggatcttcgg gcgctgccga
     3961 tcagatcaag ttccctcgac ggcatctggt gctgcgcggc actccttcat gtcccagagg
     4021 acgacacttc tggtgtcttg cacgggttca ggcgtgcgct caagccggac gggaccctcg
     4081 ccctggtcac cgccctgggt gacggtcatc ggctggaacc cgtcccgtac gctccgggcg
     4141 agcggcgatg gttcgcgtat cggcacgccg accgactcgg tcagcaactc cgagagaccg
     4201 gcttccgcgt gcgggtcgac gacgtggtcg ccagcaatcg agaatggatc accgtcctcg
     4261 ccgaggccgt ctagcggcgc ggcgtcatcg caggagtcgg ctcatgcggc ggtcggccag
     4321 gaccttgccg ccggtctgac aggtcgggca gtagacgacc tcgtgggact cgtagctcac
     4381 acgccggagg tcgtcaccgc acaccgggca gggctcgccg gcccggttgt gcaccacgaa
     4441 atggtctccc agcttcgtgg gcaggccgcc gttgcgccgc cgctcctcct cgagggcctc
     4501 gtccaggatc gagtgggtcg cggcgatcag gcgttcgcgt tcctcgggcg tgagcttggc
     4561 gaggaccgcg tagggcgaga gcttggcgca gtggaggatg tcgtcgctgt agccgcgacc
     4621 caccccggcg atcgtgcgct ggtcgcgaag gatcgtgtgc acccggcgtc cgtcgtcgct
     4681 ctcgcgcaag agggcgtcgg ccgcatcggt caagacctcg gggccgagct tgtccagcgg
     4741 cccgtcatcg ccgggcgcca gcacccacca gcccgccttc cgctccttgc cgaactcctt
     4801 caccagcacg ctgggccggt cggtgacacg gatccgcagc acggcaccgc gcggcttcgt
     4861 ctgtttcggc ggcgtctcga cgtcgacgcg cccgccttgg gacaggtgca ccagcaggcg
     4921 catcccgccg tccagctgga tcacgacgta cttgcccagg gatcgaaccg actcgatggt
     4981 gtgaccgagc gcttcgtcgg gtcgcggtgc gaacgtcttc agcgacgaga actggagctg
     5041 gtcgagcttc gcgagcgtcc cgccggtcat gatctgatcg agtcgttcgg agagcgcgcg
     5101 gatctccggc atctccggca cggtccgccc tcctcaggcc cgcagctcgt cggcggcgcg
     5161 accgacgatc tcgatgcact cgtcgatccg gtccttgtgg gtgcgatggc tcacgatgca
     5221 cacccgcagc acgtagcgtc cgccgatcat cgtgctcgac aggaacaccc gcttcgaggc
     5281 gttgatgcgg gccaggaact cgcgctgcgt gtcgtcgtcg gcgcccgcca gccggaacgg
     5341 gatcacggtg agctgcggtt cccagcacag ctcgatgcga tcgtcgtcgc ggaacgcgtc
     5401 ggccaggtag ccggcgagat ccagcttctc gtcgagcgcc tcacggaacg ccgacacccc
     5461 gtgcagcgcc agcggcatcc agacgcgcag accccgccag tcgcgggaga gctcggccga
     5521 gtgctcgctg tagttgggca gctcgccggt gggcggcaga tcctggaggt aggcggcgcc
     5581 ctcgtagtgg gcgtcgcgca tggcggctcc gtcccgcacg accaagccgc cggtgccgta
     5641 gggcaggaac aaccccttgt gcggatcgag ggtgacggag tcgctccgct cggtgccccg
     5701 gaatcgctcg cgtccgcggt cggtgagctg gaagaacccg ccgtaggcgc cgtccacgtg
     5761 cagccacagg tcctcggcct cggcgatgtc ggcgatgtcg gcgatcgggt cgatcgcgcc
     5821 cgtgttcgtg gtgcccacgc tcggcgccac caggaacggt cgcagaccgt cggcgcggtc
     5881 aagggcgatc tgcgtccgca acgcctgcgg atccatccgc agctcgtcgt ccgtcgccac
     5941 gatccgcacg ttccgcttgg agaagcccgc gatcgtggcg gccttcggca cgctggcatg
     6001 cgcctgttcg ctcacgtagt agacgccgtc ggtgaagtcc tcgccgagct tggcatggcg
     6061 ggcggtcacc atcgccgac
//
LOCUS       DATDBZ010000137         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2708246, whole genome shotgun sequence.
ACCESSION   DATDBZ010000137 DATDBZ010000000
VERSION     DATDBZ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2708246"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VIX62_03385"
     CDS             <1..1439
                     /locus_tag="VIX62_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5859307.1"
                     /translation="IAMLILSVQLTLLSLALMPLFLWLTVKVGRARREVSSSTQKTMA
                     DMTSITEETLSVSGILLSKSFGRQRHEIGRFEEENQRLTGLQIRQTMIGRSFFAVVGT
                     FFSITPALVYLVSGWVLTNGGDINYGYVVAFTTLQARLFFPIGSMLQVSTDVHSSFAL
                     FDRIFEYLDMEHEIKDAPDAAHPEPDSVRGRVRFDHVRFRYEEPVERTTPVSPTDVQI
                     ANEMPRQWTLDDVSLEIEPGQLAAFVGPSGAGKTTITYLVPRLYDVQQGTVMIDDLDV
                     RDVALESLGDLIGVVTQETYLFHTTIRRNLIYGRPTATQEEIEAAAIAANIHDRIVEL
                     PEGYDTVVGERGYKLSGGEKQRLAIARVVLKDPRILILDEATSSLDTTSERLVQAALE
                     PLMDGRTTIAIAHRLSTILRADVIFVIDRGRVVEKGTHEELLARQGLYAQLYQQQFRG
                     GLVQAECEDGVILASGEVVRTGSGSGVA"
     gene            complement(1444..1935)
                     /locus_tag="VIX62_03390"
     CDS             complement(1444..1935)
                     /locus_tag="VIX62_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5859308.1"
                     /translation="MNIQIREATPEEYAEAGAVTASAYREFVRGGDWEDYLQSIADVE
                     GRADRTTIVIALDGTRIVGSATLELTDRVEPDDDPALDPAEAHVRMLGVAPEARRRGI
                     ARALMADCMDRARAGGKTFLTLHTTDRMAAAQRMYESLGFVRGADRVFPDGFVLLTYR
                     AEL"
     gene            2022..2447
                     /locus_tag="VIX62_03395"
     CDS             2022..2447
                     /locus_tag="VIX62_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY5859309.1"
                     /translation="MEGNDLARLLLDAHRTVSAELDLALDDRGYADVRPGHAVVFLHV
                     DRRRGSRLTDLAERAGVSKQAMMLAVDELEVRGYVRRVVDPADARAKLVRLTTKGRSC
                     ATECRRAVGTMDTRTKRLLGVRRYEAMREALEILAAEGS"
     gene            complement(2444..>3229)
                     /locus_tag="VIX62_03400"
     CDS             complement(2444..>3229)
                     /locus_tag="VIX62_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859310.1"
                     /translation="ERWSAGQVRERWPSLHLPDGADIVYQPDGGVVRAARTVQVQARL
                     AADAGADVRQETTVRSVRPEGERARVLTETDGSFEAPVVVIAAGSWAAPLLRPAGFDL
                     VLRPNLEQATYFSLAPEHTLPTVIDWFTDEAHPPYLVPDPFEPGHFKVGLHMAGPPTD
                     AETRTFDPDPVRVEAVRRYVQERIEGAADLDRTDTCLYTITPDEDFVLDRVGPLVIAS
                     PCSGHGFKFVPLFGQAIADLATGEPTPFPTDAFRADRAALQAG"
BASE COUNT          502 a         1114 c         1117 g          496 t
ORIGIN      
        1 tgatcgcgat gttgatcctg tcggtgcagc tcaccctcct gtcgctggcg ttgatgcccc
       61 tgttcctgtg gctcacggtg aaggtcgggc gcgctcgtcg cgaggtctcc tccagcacgc
      121 agaagaccat ggccgacatg acctccatca ccgaggagac gctcagcgtc agcggcatcc
      181 tgctgtccaa gtccttcggt cgacagcgcc acgagatcgg gcggttcgag gaggagaacc
      241 agcgcctcac cgggctgcag atccgccaga cgatgatcgg gcgttcgttc ttcgccgtgg
      301 tgggcacgtt cttctcgatc acgccggcac tggtctacct ggtgagcgga tgggtcctca
      361 cgaacggcgg cgatatcaac tatggctacg tcgtggcgtt caccacgctg caggcgaggc
      421 tcttcttccc gatcggttcg atgctccagg tctccaccga cgtgcacagc tcgttcgcgc
      481 tgttcgatcg catcttcgag tacctcgaca tggagcacga gatcaaggac gcgcccgacg
      541 ccgcgcaccc cgagcccgac agcgtgcggg gccgggtgcg gttcgaccac gtgcggttcc
      601 ggtacgaaga gccggtggag cgcaccacgc ccgtctcacc caccgacgtc cagatcgcca
      661 acgagatgcc gcgccagtgg acgctcgacg acgtctcgct cgagatcgaa ccggggcagc
      721 tcgcagcctt cgtcgggccc agcggtgccg gcaagaccac gatcacctac ctggtaccgc
      781 ggctctacga cgtccagcag gggaccgtca tgatcgacga cctcgacgtc cgcgatgtcg
      841 cgctggaatc gctcggcgac ctgatcgggg tggtcaccca agagacctat ctgttccaca
      901 ccacgatccg ccgcaacctg atatacggac ggcccaccgc cacgcaggag gagatcgagg
      961 ccgccgcgat cgcggccaac atccacgatc gcatcgtgga gttgcccgag gggtacgaca
     1021 ccgtggtggg cgagcgcggc tacaagctgt ccgggggcga gaagcaacgc ctcgccatcg
     1081 cccgcgtggt gttgaaggac ccccggatcc tgatcctgga cgaggccacg tcctcgctcg
     1141 acaccacctc ggaacggttg gtccaggcgg cgctggagcc cctgatggac ggtcgcacca
     1201 cgatcgcgat cgcgcaccgg ctctccacga tcctgcgggc cgacgtgatc ttcgtgatcg
     1261 accgcggccg ggtggtcgag aaaggcacgc acgaggaact cctcgcccgt caggggctgt
     1321 acgcccagct gtaccagcag cagttccgcg gtgggctcgt gcaggccgaa tgcgaggacg
     1381 gcgtgatcct ggccagcggc gaggtcgtgc gcaccggcag cggttccggg gtggcctgag
     1441 ccgctacagc tcggcccggt aggtcagcag cacgaacccg tccgggaaca cgcggtcggc
     1501 gccacggacg aaccccagcg actcgtacat gcgctgggcc gccgccatcc gatcggtggt
     1561 gtggagcgtg aggaaggtct tgccgccggc ccgagcgcgg tccatgcagt cggccatcag
     1621 cgcgcgggcg atccctcgcc ggcgtgcttc tggcgccacg cccagcatgc ggacgtgcgc
     1681 ttcagccgga tcgagggcgg ggtcgtcgtc gggttccacg cgatcggtga gctccagcgt
     1741 cgcgctgccg acgatccgcg tgccgtcgag cgcgatcacg atcgtggtgc gatcggcgcg
     1801 tccttcgacg tcggcgatcg actggaggta gtcctcccag tccccgccgc gcacgaactc
     1861 gcggtaggcc gacgcggtga cggcgcccgc ttcggcgtac tcttcggggg tcgcttcgcg
     1921 gatctggatg ttcatggacc gcaggctatc gggtgacggc gggccatgac ggtcggtcaa
     1981 gcaccttgac catcgtcgtc ggcatgggga aggatgcgcg gatggaaggc aacgacctcg
     2041 ctcgtctgtt gctcgacgcg catcgcaccg tgtcggccga gctcgacctc gcgctcgacg
     2101 atcgaggcta cgccgatgtc cgtccgggtc acgccgtggt gttcctccat gtggaccggc
     2161 ggcgcggaag ccgcctcacc gacctcgccg agcgcgccgg cgtctcgaag caggcgatga
     2221 tgctggccgt cgacgagctc gaggtccgcg ggtacgtccg gcgcgtcgtc gatccggccg
     2281 atgcgcgagc caagctcgtg cggctcacca ccaagggacg gagctgcgcc accgagtgcc
     2341 ggcgggcggt gggcaccatg gacacccgca cgaagcgcct gctcggggtc cgccggtatg
     2401 aagcgatgcg cgaagcgctg gagatccttg ccgccgaggg ctcctagccc gcctgcaacg
     2461 cggcgcggtc ggcccggaag gcatcggtgg ggaacggcgt cggctccccg gtcgccaggt
     2521 ccgcgatcgc ctgaccgaac aacggcacga acttgaaccc gtgcccgctg cagggcgagg
     2581 cgatcaccag tggccccacg cggtcgagca cgaagtcctc gtccggcgtg atcgtgtaca
     2641 ggcacgtgtc cgtgcggtcg agatcggcgg cgccctcgat ccgctcctgc acgtagcgcc
     2701 gaaccgcctc caccctgacc gggtcggggt cgaacgtgcg ggtctcggcg tcggtgggtg
     2761 gcccggccat gtgcagcccc accttgaagt gcccgggttc gaacgggtcg gggaccaggt
     2821 agggcggatg ggcctcgtcg gtgaaccagt cgatcaccgt gggcaacgtg tgctcgggag
     2881 ccagcgagaa gtacgtggcc tgttcgaggt tgggccggag caccagatcg aacccagcgg
     2941 ggcggagcaa cggcgcggcc cacgatccag ccgcgatcac gacgacgggg gcctcgaacg
     3001 acccgtccgt ctcggtgagc acacgggcgc gctcgccctc cggcctcacc gagcgcacgg
     3061 tggtctcttg gcgcacgtcg gcacccgcgt cggcggcgag ccgggcctgc acctggacgg
     3121 tccgagccgc ccgcaccacg ccgccgtcgg gctggtagac gatgtcggcg ccgtcgggca
     3181 gatgcagcga cggccagcgt tcccgcacct ggcccgccga ccatcgttc
//
LOCUS       DATDBZ010000138         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_867231, whole genome shotgun sequence.
ACCESSION   DATDBZ010000138 DATDBZ010000000
VERSION     DATDBZ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867231"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..983
                     /locus_tag="VIX62_03405"
     CDS             42..983
                     /locus_tag="VIX62_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859311.1"
                     /translation="MLTFIIRRILAGIVLLFVISFIAFLFLYAGSGDIARKILGQNAT
                     AETVAQKAEELGLNRPILVQYWDWLTSAVTGDFGRSWFTGQLVSVSLSSRLAVTLSLV
                     VGAILVSAILSVLLGVLAARRGGGVDGTVQFISMLGFAIPGFLVALFLVLIFAINLGW
                     FKATGYIPITTSFTGWLSSVTLPIAALALGAIASVTQQVRGSVIDGLSRDYVRTLRSR
                     GLSSNRVVYKHVLRNAGGPALAVLSVQFIGLLGGAIIVEQVFALPGMGQLTVSATTVG
                     DIPVVMGVVIVFAVIVVIVNLLIDLAQAALNPKVRLS"
     gene            980..>2792
                     /locus_tag="VIX62_03410"
     CDS             980..>2792
                     /locus_tag="VIX62_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HEY5859312.1"
                     /translation="MTAVEIPTDVPTPAAAAPDHLFRRLIRRPLGLISILFLALVLLI
                     AIIGPWITPYDPNLASLQLILAPPSPEHLLGADGAGRDVLSRLLAATRTTIFAALLAV
                     ITAFIIGVISGLIAGYYKGWFEGTAAWLTSLVMALPGLVVLLAARAIVGPSVWYAMLI
                     FGVLLAPAYYRLVFAAVTAVRSELYVDAARVAGLSDVRIISRHILSVVRAPIVIQTAI
                     LTGIAIGVQSGLEFLGLGDRSVPTWGGMLNDAFTNIYKAPLLMLWPSLAIALTCISLT
                     LLANAMRDELERVVAVRRKRRRAVATATGSVAAVTTAVSVKGVAEIEDLDEAPVIEGS
                     AGPILHEDDHRTSLRSQEAVLTITDLRVGYGETLEVVHGVSMQIRKGEIHGLIGESGS
                     GKSQTAFAVLGLLPRGGHVTGGTIDYEGTLLDDNDERTYAGIRGRRIGYIPQEPMSNL
                     DPSFTIGSQLVEPLRVALKLSRKDATEKALALLDRVGIPDPKRTFAAYPHEISGGMAQ
                     RVLIAGAVSTDPDLIIADEPTTALDVTVQAEVLDVLRGLQEERHMAMLLVTHNFGVVA
                     DLCDRVTVMQAGRFVESGPARTILTKPHHPYTQSLLDA"
BASE COUNT          399 a         1014 c          884 g          495 t
ORIGIN      
        1 ggggggggcg ggccgggaac ccaaccacag gagctcatcg catgctcacg ttcatcatcc
       61 ggcgcatcct cgccggcatc gtcctcctct tcgtcatctc gttcatcgcc ttcctcttcc
      121 tctacgcggg cagcggcgac atcgcccgca agatcctcgg acagaacgca acggccgaga
      181 ccgtggcgca gaaggcggag gagctgggtc tcaatcgccc gatcctcgtg cagtactggg
      241 actggctgac ctccgcggtc accggcgact tcgggcggtc gtggttcacc ggccaactcg
      301 tatccgtcag cctctccagt cgtctcgcgg tcaccctctc gctcgtggtc ggggcgatcc
      361 tcgtctccgc gatcctgtcg gtgctcctgg gcgtgctggc ggcgcggcgg ggtggcgggg
      421 tcgacggcac cgtccagttc atctccatgc tcggcttcgc gatccccggc ttcctcgtcg
      481 ccctcttcct ggtgctgatc ttcgcgatca acctgggctg gttcaaggcg accgggtaca
      541 taccgatcac gacatccttc accggctggc tgtcgtcagt caccctgccc atcgcagctc
      601 ttgccctcgg ggcgatcgcc tcggtgacgc agcaggtgcg aggctcggtc atcgacggac
      661 tgtctcgcga ctacgttcga acgctccgca gtcggggcct cagcagcaat cgcgtcgtct
      721 acaagcacgt gctgcgcaac gccggcggcc ccgcgctcgc ggtgctctcg gtgcagttca
      781 tcggcctcct cggtggcgcg atcatcgtgg agcaggtctt cgccctcccg gggatgggcc
      841 aactgacggt gtcggcgacc accgtgggcg acatcccggt cgtgatgggc gtcgtgatcg
      901 tcttcgccgt catcgtcgtc atcgtcaacc tcctcatcga cctcgcgcag gccgcgctca
      961 acccgaaggt gcgactctca tgaccgctgt cgaaattccc accgacgttc ccaccccggc
     1021 ggcggcagcc cccgatcacc tgttccgcag gctgatccgg cgtccgctcg gtctgatctc
     1081 catcctgttc ctcgccctgg tgctgctcat cgccatcatc ggcccgtgga tcaccccgta
     1141 cgaccccaac ctcgcttcgc ttcagctgat cctcgccccg ccgagtcccg agcacctcct
     1201 cggtgccgac ggcgccggtc gagatgtgct ctcgcggctg ctggcggcga cgcgcaccac
     1261 gatcttcgca gccctgctcg ccgtgatcac agccttcatc atcggcgtga tctcaggact
     1321 gatcgccggc tactacaagg gctggttcga gggcaccgcc gcgtggctga cgtcgctcgt
     1381 gatggcgctc cccggactcg tggtgctgct ggccgcccgt gccatcgtcg ggccatccgt
     1441 gtggtacgcg atgctcatct tcggtgtgct actcgcgccg gcctactacc gactggtgtt
     1501 cgcggcagtc acggcggtgc gctccgagct ctacgtagat gcggcgcgag tcgcgggcct
     1561 gagcgacgtg cgcatcatca gccgtcacat cctctcggtg gtccgcgcgc cgatcgtcat
     1621 ccagacggcg atcctcaccg gcatcgcgat cggggtgcag tcgggactgg agttcctggg
     1681 gctcggcgac cggtcggtac ccacgtgggg tggaatgctg aacgacgcgt tcaccaacat
     1741 ctacaaggcg ccactgctca tgctctggcc gtcgctcgcg atcgcgctga cctgtatctc
     1801 gctcacgctg ctcgccaacg cgatgcgcga tgagctcgag cgggtcgtcg cggtccgccg
     1861 caagcgccgc cgggccgttg cgaccgcgac cggctccgtc gcggccgtca cgacggcggt
     1921 ctccgtgaag ggcgtcgccg agatcgagga cctcgacgag gcgccggtga tcgaagggag
     1981 cgccggtccg atcctgcacg aggacgacca tcgcacttcg cttcgcagcc aggaggccgt
     2041 gctgacgatc accgatcttc gcgtcggtta cggcgagacg ctcgaggtcg tccacggcgt
     2101 ctcgatgcag atccgcaagg gcgagatcca cggcctcatc ggcgaatcgg gctcgggcaa
     2161 gtcgcagacg gcgttcgcgg tgctcggtct gctcccacga ggcggtcacg tcacgggtgg
     2221 cacgatcgac tacgagggca cgctgctgga cgacaacgac gagcgcacct acgcggggat
     2281 ccgcgggcgc cgcatcgggt acatccccca ggagccgatg tcgaacctcg acccgtcgtt
     2341 cacgatcggc agccagctgg tcgagccgct ccgcgtcgcc ctcaagctca gcaggaagga
     2401 tgccaccgag aaggcgctcg cgctgctcga ccgcgtcggc atccccgacc cgaagcgcac
     2461 gttcgccgcc tacccgcacg agatctcggg cggcatggcg cagcgcgtgc tcatcgcggg
     2521 cgccgtgtcg accgaccccg acctgatcat cgccgacgag ccgaccacgg ccctcgacgt
     2581 gaccgtgcag gccgaggtgc tcgacgtgct gcgcggcctc caggaggagc gccacatggc
     2641 gatgctcctc gtgacccaca acttcggcgt cgtcgccgac ctgtgcgacc gcgtgaccgt
     2701 catgcaggcg ggcaggttcg tggaatcggg ccccgcgcgc accatcctga cgaagccgca
     2761 ccacccttac acgcagtcgc tgctcgacgc ga
//
LOCUS       DATDBZ010000139         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1481549, whole genome shotgun sequence.
ACCESSION   DATDBZ010000139 DATDBZ010000000
VERSION     DATDBZ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481549"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..408
                     /locus_tag="VIX62_03415"
     CDS             70..408
                     /locus_tag="VIX62_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5859313.1"
                     /translation="MVIREIGVIDEACCTPLVVAPLSEDDAERLASALRVVADPARLR
                     LLSLITASGEACVCDLTEPLGLSQPTVSHHLKVLTDAGLLAREQRGRWAFFRLQPHGL
                     DLLRDALARS"
     gene            complement(405..1586)
                     /locus_tag="VIX62_03420"
     CDS             complement(405..1586)
                     /locus_tag="VIX62_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5859314.1"
                     /translation="MGPSKLHASAKASSFTESVIRDMTRLANLHGAINLGQGFPDFAA
                     PRPLKDAAAQAIADDHNQYPITWGVPAFREAIAAKYLREYGMTVDPETEICVTCGSTE
                     AMIATMLGVLDPGDEVVIFEPFYENYGPDSILSGAVPKLVTLRPPGWTFDEAELRAAF
                     TDRTRAIVINSPNNPTGKVFTREELSLIADLCVQHDAIAITDEIYEHITYDGARHVPI
                     ATLDGMAERTVTISALSKTYSVTGWRVGWAIASPTLMGGIRSVHDFLTVAAAAPLQVA
                     GITALELPASYYAEASAEYAERRDLMMTILAETGFEAASPAGAYYVMADCSHLGLGDD
                     VATARHLVEHVGVATVPGSSFFSKPEDGAHLVRFAFCKNLATLEAAGERLRALHGSGP
                     Y"
     gene            complement(1599..>2068)
                     /locus_tag="VIX62_03425"
     CDS             complement(1599..>2068)
                     /locus_tag="VIX62_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5859315.1"
                     /translation="HRASGHLPQPSFIEERTHAWAIGDRVAWEEAEPSMPDPWLDRLF
                     ALRRPVSLPSQLIHGDLTDNVLFDDELAPAIIDPTPYWRPAAFASAIVVHDAIRWWSA
                     DPEPLIAATAHIEEFPQLFVRAAIYRMVTSIIVGSDDPEDRREVVDLATWLAG"
BASE COUNT          333 a          685 c          710 g          340 t
ORIGIN      
        1 gacacctcct ctcatatcga gtgcggtcga tacgttggca gatacatcga tgcgtgtcaa
       61 tatatgggca tggtgatcag ggagatcggc gtgatcgacg aggcgtgctg cacgcccttg
      121 gtggtggccc cactgtccga agacgacgcg gagcgcttgg cctcggcgct tcgggtggtg
      181 gccgatcccg cccggctccg tctgctgagc ctcatcacgg cgtcgggcga agcctgcgta
      241 tgcgatctca cggagccgct cgggctctcc cagccgaccg tcagccatca cctcaaggtg
      301 ctcaccgacg ccggcctgct ggcacgtgag caacgcggtc gctgggcgtt cttccgcctc
      361 caaccgcacg gcctggatct gctccgagac gcgctcgcac ggagctagta cggaccggac
      421 ccgtggagcg cgcggagacg ttcaccggcg gcctcgagcg tcgccaggtt cttgcagaac
      481 gcgaaccgca ccaggtgggc gccgtcttcg ggcttggaga agaacgacga acccggcacg
      541 gtggccacgc ccacgtgttc gacgaggtgc cgggcggtcg cgacgtcgtc gcccagcccc
      601 aggtgtgagc agtcggccat cacgtagtac gcaccagccg gcgaggccgc ctcgaacccc
      661 gtttccgcga ggatcgtcat catcaggtcg cggcgttcgg cgtactcggc ggacgcctcc
      721 gcgtagtagc tggccgggag ttcgagcgcg gtgatgcccg ccacctgcag cggggccgct
      781 gctgcaacgg tgaggaagtc gtgcacggag cggatgcctc ccatcagcgt cggggacgcg
      841 atcgcccacc ccacgcgcca tcccgtcacc gagtaggtct tcgacagagc gctgatcgtc
      901 acggtgcgct cggccatgcc gtccagcgtg gcgatcggga cgtggcgcgc gccgtcgtag
      961 gtgatgtgct cgtagatctc gtcggtgatc gcgatcgcgt cgtgttggac acagaggtcc
     1021 gcgatcagcg acagctcctc gcgggtgaac accttcccgg tggggttgtt gggcgagttg
     1081 atcacgatcg cacgggtccg gtccgtgaac gccgcacgca gttcggcctc gtcgaacgtc
     1141 cagccgggcg gacgcagggt gacgagcttc gggacggcgc cggacaggat cgagtccggg
     1201 ccgtagttct cgtagaacgg ctcgaagatc acgacctcgt cgccgggatc gagcaccccg
     1261 agcatcgtcg cgatcatcgc ctcggtggaa ccgcacgtga cgcagatctc ggtctcggga
     1321 tcgaccgtca tgccgtattc gcgcaggtac ttcgcggcga tggcctcgcg gaacgcgggc
     1381 accccccagg tgatcgggta ctggttgtga tcgtcggcga tcgcctgagc ggcagcgtcc
     1441 ttcagcggcc gcggcgccgc gaagtccggg aacccctgcc ccaggttgat cgccccgtgc
     1501 aggttcgcca gacgcgtcat gtcccggatc accgactcgg tgaatgacga cgccttcgcc
     1561 gaggcatgca gcttcgaggg acccatgggg cccagagcct atccggccaa ccaggtcgcg
     1621 agatcgacga cctctcgtcg atcctccggg tcatcggatc cgacgatgat gctcgtcacc
     1681 atccggtaga tcgcggcgcg gacgaacagc tgcgggaact cctcgatgtg cgccgtcgcg
     1741 gcgatcagtg gttcggggtc ggcggaccac cagcgaatcg cgtcgtgaac cacgatggcg
     1801 ctggcgaacg cggcaggtcg ccagtagggc gtcgggtcga tgatcgcggg cgccagctcg
     1861 tcgtcgaaca gtacgttgtc ggtgagatca ccgtggatga gctgcgacgg cagcgagacc
     1921 ggacggcgca gcgcgaacaa ccggtccagc cagggatccg gcatcgatgg ctccgcctcc
     1981 tcccacgcga cccgatcgcc gatcgcccaa gcgtgcgttc gttcctcgat gaatgacggc
     2041 tgaggcaggt gcccggacgc ccgatgga
//
LOCUS       DATDBZ010000140         7545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_27431,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000140 DATDBZ010000000
VERSION     DATDBZ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27431"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1040)
                     /locus_tag="VIX62_03430"
     CDS             complement(117..1040)
                     /locus_tag="VIX62_03430"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859316.1"
                     /translation="MARKDDRPGGVWYPRWFWPSFAAPATIWLLLFFVLPFYVVLSVA
                     FGRLDPIFLTAEPVYNPVQWDTTPFRGVVTRLFTAGSIEQETLVRTLVYVGIATAACL
                     LIGYPVAYFVARHAKRTKTWLLVAFIAPFWISYMMRMFAWVNLLQEDGYVNRFLEAIG
                     VIDHPEQWLSGRASTVVFGLIYGYIPFMILPLFGTLDRMDRSMIEAARDLGAGQVKTF
                     FRVTLPLSKQGILAGCIIVALPMFGDYFTQTLLSSTRNTAMFGNLIVSSIQSSLVNTG
                     AALVVVMVVLLIVPMLYYLRSTNVARELAER"
     gene            complement(1030..2298)
                     /locus_tag="VIX62_03435"
     CDS             complement(1030..2298)
                     /locus_tag="VIX62_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5859317.1"
                     /translation="MAHPKDRPRPIDRRAFLQRAAAAGIALPSMAAILAACGDSGSSG
                     GDASASAALQLARPDNPVTLPISADNPAIADGLDPESGPLKIFGYNDYIWKKVLNGFG
                     EQYGVDVEYTVFDTPDEMVSKMQSNGADFDAVVTVTLENIGKLAQGNLIQPLNKTYLT
                     TATDQLSPGIPDFYDVGRQYSMPYVIFTTGIAWRNDEVSEDIAALDNPWDVFWDPKYK
                     GQAHLLNGSRDTLAVGLLRKGYDPNESDQTILDQVKQDLLAGADEMGWKYDHVDYTEL
                     STNLWQVHNTWSGQMAYYQYYLPKGLDITALSYAWPPQGQGGQKGLISHDLFAIPTGA
                     ASPVLAHALIDYLYDPKNAITNYSYEGYQPTVKSFNEDVAVSDGFLPESLTYTIVTED
                     MVPMGVSELELEPAINQLYQQIYQEVTGGA"
     gene            2479..4311
                     /gene="typA"
                     /locus_tag="VIX62_03440"
     CDS             2479..4311
                     /gene="typA"
                     /locus_tag="VIX62_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY5859318.1"
                     /translation="MPTRNDIRNVAIVAHVDHGKTTLVDAMLWQSGAFRENQDVNERV
                     MDSMDLEREKGITILAKNTAVRYGDIKINIVDTPGHADFGGEVERALTMVDGVLLLVD
                     ASEGPLPQTRFVLRKALESRLPVILVVNKVDRPDARVGEVVHEVEELFLDLDADEHQI
                     DFPILYANARSGQAGEDPETLAADLKPLFETLLTYIPAPEYEEGHPLQALVTNLDASP
                     YVGRLAMCRVRNGSITRGQAVAWCRADGTIEQAKIGELYVTDALDRVDAEEAGPGEII
                     AVAGIAEITIGETLADPADPRPLPVTSVDEPSLSMTIGINTSPLSGTDGDKMTARLLK
                     GRLDQELVGNVSIRVLETERPEMWEVQGRGELQLAVLVEMMRREGFELTVGKPQVVTR
                     TVNGQLQEPMERVAIDAPEDYLGVISQLLALRKGRMEQMVNHGTGWIRMEYLVPARGL
                     IGFRTEFMTETRGTGQLHHVFEGYEPWHGELRTRPSGSLIADRTGVTTQFALLNLQER
                     GSMFVGPGEDVYEGMIIGENARADDMDVNPTKEKHLTNMRKANAEELVRLIPKLQLSL
                     EQALEFIREDECVEVTPNHVRLRKVLLDQQGRVKAGRRAKPAKD"
     gene            complement(4317..4607)
                     /locus_tag="VIX62_03445"
     CDS             complement(4317..4607)
                     /locus_tag="VIX62_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859319.1"
                     /translation="MSVRHAEQTVIIDEGTDDAFALAQRVLDEIGTVSHVDLEGRFLQ
                     GTTRSGFQRVRVGITARPTSRGTALVVEASSHGTRSQARKGVSRVVGALGVV"
     gene            4829..5911
                     /locus_tag="VIX62_03450"
     CDS             4829..5911
                     /locus_tag="VIX62_03450"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005446593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY5859320.1"
                     /translation="MPKRTIAGDLPAPMLAKVQPDMPIGDGWTYEPKWDGFRSIVAID
                     TDGTARLASRDGRPLGRYFPELLELLERDASHGPFVADAEIVRVAPGRMDFDELQLRL
                     HPAESRVRKLAGEIPATLIVFDLLWLGDEGLTEVPLAERRERLTTLASAMGWEDAPGS
                     LDDVPTGPTVLAAPWTDDPEVARGWFADDAGIGQDGIIAKRAEQPYLSGERGWIKVKH
                     RSTLDCVVGGYRLSKTGDGIGALLLGLYDGDGTLHYVGHTSSFKAKERRDILEQLRPL
                     EGGDSFEGGRAPGGQSRWSAGKDTAWITLQPVLVCEVTIDRVQYGRFRHAASFVRWRD
                     DREPASCTFDQIPGAEPPAWPEPTDG"
     gene            complement(5908..7032)
                     /locus_tag="VIX62_03455"
     CDS             complement(5908..7032)
                     /locus_tag="VIX62_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY5859321.1"
                     /translation="MATPATSIEVQGREIRVTNPDKVYFPDAEGGPITKLEVVEYWAS
                     VAEAALHGCRDRPEILHRFPAGVADDGEGSFYQKRIPKGSPEWVQTATVMFPSGRTAA
                     MPVMADEAALVWASTLGCLECNPWPVRADDVAHPDELRVDLDPTPGVPWDDVRRVALM
                     SRDVLAEHGLTGFPKTSGKRGMHINARVERGNGFTATRRAALAFAREVERRMPGVATT
                     AWWKEERQGVFLDYNQNARDRTVASAYSVRPMPDARVSTPLAWDEVGDVDPSRFTVRT
                     VPGRLLQIGDPGAGIDQQVGSLDALLTLAEQDAAGGLGDAPWPPHFPKGDDEPVRVNP
                     SRAKKIAEWEEMKRQGLGGDRGQGSQGRGQGRRRKEPGSA"
     gene            7140..>7545
                     /locus_tag="VIX62_03460"
     CDS             7140..>7545
                     /locus_tag="VIX62_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY5859322.1"
                     /translation="MDPLLTDTQDLIGRVQTLHREIAERQRELLEVLAQLETRRAWVE
                     DGAHDMAQWTSMALDVSRWKADRLLGAGRALPTLPATAHALARGDLGIDKVVELARFA
                     TPETESRLIPWARAVSCGTVRHRGDIETKPSID"
BASE COUNT         1245 a         2562 c         2550 g         1188 t
ORIGIN      
        1 ccacgcgagg tagccgaaca ccgccaccca caggaaccgg gcctgggccc acgggttggc
       61 gatccacgag gtcaatcgac ggctcgccgg aggtgcctgc accgagagcg ggccactcat
      121 cgttccgcca gctccctcgc gacgttcgtg ctgcgcaggt agtagagcat cggcacgatc
      181 aacagcacga ccatgaccac caccaacgcg gcgcccgtgt tcaccagcga gctctggatc
      241 gaactgacga tgaggttgcc gaacatcgcc gtgttgcgcg tgctggagag caacgtctgg
      301 gtgaagtagt cgccgaacat cggcagcgcc acgatgatgc agccggccag gatgccctgt
      361 ttcgacaacg gcaacgtgac gcggaagaac gtcttgacct ggccggcgcc caggtcgcgc
      421 gccgcttcga tcatcgagcg gtccatgcga tcgagcgtgc cgaacaacgg caggatcatg
      481 aacgggatgt agccgtagat cagcccgaac accacggtgg aggctctccc gctcagccac
      541 tgttcgggat ggtcgatcac cccgatcgcc tcgaggaacc ggttcacgta gccgtcctcc
      601 tggagcaggt tcacccaggc gaacatccgc atcatgtagc tgatccagaa cggcgcgatg
      661 aacgccacca gcagccaggt cttcgtgcgc ttggcgtgcc gcgccacgaa gtaggccacg
      721 gggtagccga tcagcaggca tgccgccgtc gcgatgccca cgtagaccag cgttcggacc
      781 aaggtctcct gctcgatgga gccggcggtg aacagcctgg tcaccacccc gcggaacggc
      841 gtggtgtccc actggaccgg gttgtaaacc ggttcggccg tcaggaagat cgggtcgagg
      901 cgcccgaacg ccaccgagag caccacgtag aacggcagga cgaagaacag caggagccag
      961 atcgtggccg gtgccgcgaa cgacggccag aaccagcggg ggtaccagac ccccccgggc
     1021 cggtcgtcct tacgcgccac ccgtcacttc ttggtagatc tgctggtaga gctggttgat
     1081 cgcgggctcc agctccagct ccgacacgcc catcggcacc atgtcctcgg tgacgatcgt
     1141 gtaggtgagg ctctcgggca ggaagccgtc ggagaccgcg acgtcctcgt tgaacgactt
     1201 caccgtgggc tggtagccct cgtaggagta gttggtgatg gcgttcttgg ggtcgtacag
     1261 gtagtcgatc agcgcgtggg ccagcaccgg gctcgcggcg cccgtcggga tcgcgaacag
     1321 gtcgtgggag atcaagcctt tctgtcctcc ctggccctgc ggtggccacg cgtagctgag
     1381 cgccgtgatg tccaggccct tgggcaggta gtactggtaa taggccatct gtcccgacca
     1441 cgtgttgtgc acctgccaca ggttcgtgct gagctcggtg tagtcgacgt ggtcgtactt
     1501 ccagcccatc tcgtccgcgc cggccagcag gtcctgcttg acctggtcca ggatcgtctg
     1561 gtcgctctcg ttcgggtcgt agcccttgcg caacagcccc accgccaagg tgtcgcgcga
     1621 gccgttcaac aggtgggcct ggcccttgta cttcgggtcc cagaacacgt cccacgggtt
     1681 gtcgagcgcc gcgatgtcct cgctgacctc gtcgttgcgc cacgcgatcc cggtggtgaa
     1741 gatcacgtag ggcatcgagt actggcggcc cacgtcgtag aagtccggga tccccggcga
     1801 cagctgatcg gtggcggtgg tgaggtaggt cttgttgagc ggctggatca ggttcccttg
     1861 cgcgagcttg ccgatgttct cgagcgtcac ggtcacgacc gcgtcgaagt cggcgccgtt
     1921 ggactgcatc ttcgacacca tctcgtcggg tgtgtcgaac accgtgtact ccacatccac
     1981 gccgtactgt tcgccgaacc cgttgaggac cttcttccag atgtagtcgt tgtaaccgaa
     2041 gatcttcagc ggccccgact ccggatcgag tccgtcggcg atcgccgggt tgtcggcgct
     2101 gatcggcagc gtgacggggt tgtcgggtcg cgccagctgg agcgccgccg aggcgctggc
     2161 atcccctccg ctcgagccgg agtccccgca cgccgcgagg atcgcggcca tgctcggcaa
     2221 cgcgataccg gcagcggctg cccgctgcag gaacgctcgc cggtcgatgg gtcgggggcg
     2281 gtctttcgga tgcgccatgg tcgaaccttt ctcgtcggca ggtcgctgtg ggctgtttct
     2341 acacccctga cacgtccggc gtgcgcgggc ggggggtcgg gcgatgtccc ggggtcaggc
     2401 ccgcgggtcg actcggacca ggccgcgcgg tccgcgcgcc gatcccttcc ctccctcgat
     2461 cccggagtga cgaacgccat gcccacccgc aacgacatcc gcaacgtggc catcgtggcc
     2521 cacgtcgacc acggaaagac caccctcgtc gacgccatgc tctggcagtc cggcgccttc
     2581 cgcgagaacc aggacgtgaa cgaacgcgtc atggactcca tggacctgga gcgcgagaag
     2641 ggcatcacga tcctggccaa gaacacggcg gtgcgctacg gcgacatcaa gatcaacatc
     2701 gtcgacacgc ccggtcacgc cgacttcggt ggcgaggtgg agcgcgcgct cacgatggtg
     2761 gacggggtcc tgctgttggt cgacgcgagc gaaggtcctc tgcctcagac ccgcttcgtg
     2821 ctccggaaag cgttggagtc gcgcctgccg gtgatcctgg tggtgaacaa ggtcgaccgc
     2881 ccggacgccc gcgtgggtga agtggtgcat gaggtggagg agctgttcct ggacctggac
     2941 gccgacgagc accagatcga tttcccgatc ctgtacgcga acgcccggtc cggccaggcg
     3001 ggggaggacc cggagacgct cgcggccgac ctcaagccgc tgttcgagac cctgctcacc
     3061 tacatccccg cgcccgagta cgaggagggc cacccgctcc aagccctcgt cacgaacctc
     3121 gacgcctcgc cctacgtcgg tcgcctggcc atgtgccgcg tccgcaacgg atcgatcacg
     3181 cgcggccaag cggtggcgtg gtgccgcgcc gacggcacga tcgagcaggc caagatcggt
     3241 gagctctacg tcaccgatgc cctcgatcgc gtcgacgcgg aggaggccgg tcccggggag
     3301 atcatcgccg tcgcggggat cgcggagatc acgatcggag aaacgctggc cgatcccgcc
     3361 gatcctcggc cgctgccggt caccagcgtc gacgagccca gcctgtcgat gacgatcggt
     3421 atcaacacct cgccgctgtc gggcaccgac ggcgacaaga tgacggcccg cctcctcaag
     3481 ggacgcctcg accaggaact ggtcggcaac gtgtcgatcc gcgtgctcga aaccgaacgg
     3541 cccgagatgt gggaggtgca gggccgcggc gaactacagt tggcggtcct ggtggagatg
     3601 atgcggcgcg aaggcttcga actcacggtg ggcaagccgc aggtcgtgac gcgcacggtc
     3661 aacggccagc tgcaggagcc gatggaacgg gtggcgatcg acgcgcccga ggactacctg
     3721 ggggtgatca gccagctgct cgcgctgcgc aaagggcgca tggaacagat ggtgaaccac
     3781 ggcaccggct ggatccgcat ggagtacctg gtgccggccc gtgggttgat cgggttccgc
     3841 accgagttca tgaccgagac ccgcgggacc ggccagctcc accacgtgtt cgagggctac
     3901 gagccctggc acggcgaact ccgcacgcgt cccagcggct ccctcatcgc cgaccgaacg
     3961 ggcgtcacca cgcagttcgc cctgctgaac ctgcaggagc gcggctcgat gttcgtgggc
     4021 ccaggcgagg acgtgtacga aggcatgatc atcggcgaga acgcgcgggc cgacgacatg
     4081 gacgtgaacc ccacgaagga gaagcacctc acgaacatgc gcaaggccaa cgccgaagag
     4141 cttgtccgtt tgatcccgaa gctgcagctg tcattggagc aggcgctgga gttcatccgc
     4201 gaggacgaat gtgtcgaggt caccccgaac cacgtgcggc tgcgcaaggt cttgctcgac
     4261 cagcaggggc gcgtgaaggc cgggcgtcgc gccaagccgg cgaaggacta gcggtctcag
     4321 acgacgccga gcgcgccgac cactcggctg acgcccttgc gggcctgact gcgggtgccg
     4381 tgcgaggacg cttccaccac cagcgcggtg ccgcggctcg tcggccgggc ggtgatcccc
     4441 acccggaccc gctggaaccc tgagcgcgtg gtgccttgga ggaagcggcc ctccaggtcg
     4501 acatgcgaga cggtgccgat ctcatcgagg acccgctggg cgagcgcgaa cgcgtcgtcg
     4561 gttccttcgt cgatgatcac ggtctgctct gcatgacgta ccgacacggg gagctccttc
     4621 gttcgcgggg gtggagcgct cccaccgttg cacgttccgc cgtcgcacgc atcccccact
     4681 ggccgcaaga tcgccaatcg tcaccggatg gtgacatggg cgccatctca tgagcgaaga
     4741 cgaccagcgt ggtgcgcgtg ggggcaaccc ttggctcggc gctggggcgc gcatgaggtc
     4801 gtggtccggc gcgcgatagg gtcggcccat gccgaagcgc acgatcgctg gtgatcttcc
     4861 ggcgccgatg ctcgccaagg tccaaccgga catgcccatc ggtgatggct ggacctacga
     4921 gcccaagtgg gacggattcc ggtcgatcgt cgcgatcgac accgacggca ccgcgcgcct
     4981 cgcgagccgc gacggcagac ccttgggacg gtacttcccg gagttgctcg agctgctgga
     5041 acgcgatgcg agccacggac cattcgtggc cgacgccgag atcgtgcggg tggcgccggg
     5101 ccggatggac ttcgacgaac tgcagctgcg gctgcacccc gccgagtctc gggtgcgcaa
     5161 gctcgccggg gagatccccg ccaccttgat cgtgttcgac ctgctgtggc tcggggacga
     5221 aggactcacc gaggtcccgc tcgccgaacg gcgcgagcga ctcacgacgc tggcctcagc
     5281 gatgggatgg gaggacgcgc ccggttccct ggacgacgtg cccaccgggc ccacggtgtt
     5341 ggccgctccc tggaccgacg atccggaggt ggcgcgcggc tggttcgccg acgacgcggg
     5401 catcggccag gacggcatca tcgccaaacg tgccgagcag ccgtacctgt cgggcgaacg
     5461 cggctggatc aaggtgaagc accgctccac gctcgattgc gtcgtgggcg gctaccgcct
     5521 gtcgaagacc ggggacggga tcggtgcgct gttgctcggc ctgtacgacg gagacggcac
     5581 gctgcactac gtcgggcaca cgtcttcgtt caaggcgaag gagcgacggg acatcctcga
     5641 acagttgcga ccgctcgaag gtggcgacag cttcgaaggt ggtcgcgcgc cgggcgggca
     5701 gagccggtgg agcgccggca aggacaccgc gtggatcacc ctgcaaccgg tgctggtctg
     5761 cgaggtcacg atcgaccggg tgcaatacgg aaggttccgc cacgcagcga gcttcgtccg
     5821 ctggcgcgac gatcgcgagc ccgcgagctg caccttcgac cagatcccgg gggccgaacc
     5881 gcccgcgtgg cccgaaccga cggacggcta ggccgaaccc ggctctttcc ggcgacggcc
     5941 ctggccacgg ccctggcttc cctggcctcg atcgccgccc agaccttggc gcttcatctc
     6001 ctcccattcg gcgatcttct tcgcgcgcga agggttcacc cgcaccggtt cgtcgtcgcc
     6061 cttcgggaaa tgcggtggcc acggcgcatc gcccaggccg ccggcggcgt cctgctctgc
     6121 gagcgtcagc aatgcgtcga ggctgcccac ctgctgatcg atcccggcac ccggatcgcc
     6181 gatctgaagc aaccggccgg gcacggtccg caccgtgaag cgcgagggat cgacgtcgcc
     6241 gacctcgtcc caagccagcg gtgtcgacac gcgagcgtcg ggcatgggcc ggaccgaata
     6301 cgcactcgcc acggtgcggt cgcgcgcgtt ctggttgtag tcgaggaaca cgccctgacg
     6361 ttcttccttc caccacgcgg tggtggccac gcccggcatg cgtcgctcca cctcgcgcgc
     6421 gaaggccagc gccgcgcgcc gggtcgccgt gaagccgttc cctcgctcca cccgggcgtt
     6481 gatgtgcatg ccccgtttgc cgctggtctt cgggaacccg gtgaggccgt gttcggccag
     6541 gacgtcgcgc gacatcagtg ccaccctccg cacgtcgtcc cacggcacac cgggcgtggg
     6601 atcgagatcc acccgtaact catcggggtg agcgacgtcg tcggcacgta ccggccaggg
     6661 attgcattcc aggcagccca gcgtcgaggc ccataccagc gccgcttcgt cggccatcac
     6721 cggcatcgcg gcggtgcgtc cgctcgggaa catcacggtg gcggtctgca cccactcggg
     6781 cgaccccttg gggatgcgct tctgatagaa ggagccctcg ccgtcgtcgg ccacccccgc
     6841 ggggaagcgg tgcaggatct ccgggcggtc acggcagccg tgaagcgcgg cttcggccac
     6901 cgaggcccag tactccacca cctcgagctt cgtgatcggg ccgccctcgg cgtcggggaa
     6961 gtacaccttg tcggggttcg tgacgcggat ctcccgcccc tgcacctcga tcgaggtcgc
     7021 cggtgtcgcc atgggagagc cacgttacct gtccggcgtc gtgggacttg acatccgaac
     7081 gtatgttcga gtagcatgca gcgtcgtcca ccacccgtcc tcccccggaa cggatgcacg
     7141 tggatccact gctcaccgac acgcaggacc tcatcggccg cgtccagacc ctgcaccgcg
     7201 agatcgccga acgtcagcgc gaactgctgg aggtcctcgc ccagctcgag acccggcggg
     7261 cctgggtcga agacggcgcc cacgacatgg ctcagtggac gtcgatggcg ctggacgtct
     7321 cccgctggaa agccgaccgg ctgctcggcg ccggcagggc gctccccacg ttgcccgcca
     7381 ccgctcacgc gctcgcgcgt ggcgatctcg gcatcgacaa ggtcgtggag ctcgctcggt
     7441 tcgcgacgcc cgagaccgaa agccgcctga tcccatgggc gcgagcggtg tcgtgcggca
     7501 cggtgcggca tcgcggcgac atcgagacga agccctcgat cgacg
//
LOCUS       DATDBZ010000141         3213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1688245, whole genome shotgun sequence.
ACCESSION   DATDBZ010000141 DATDBZ010000000
VERSION     DATDBZ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688245"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VIX62_03465"
     CDS             <1..676
                     /locus_tag="VIX62_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5859323.1"
                     /translation="TDLTDRISDRLPVFIAAVVALSFLLLMIVFRSVLVPLKAAVMNL
                     LSIAAAYGVLVAVFQWGWGAELIGLHSTMPINPFLPLIMFAILFGLSMDYEVFLLSRV
                     REEYVKTGDNHEAVVRGLSSTARVITSAALIMISVFGAFVLSDDPNGKLFGVGLAVAV
                     LIDATLVRMVLVPATMSLLGRANWWLPSWLDRVLPHLDLEGGGPTVPEVSPEPEPEPE
                     RELAPV"
     gene            complement(677..1158)
                     /locus_tag="VIX62_03470"
                     /pseudo
     CDS             complement(677..1158)
                     /locus_tag="VIX62_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
     gene            1216..2016
                     /locus_tag="VIX62_03475"
     CDS             1216..2016
                     /locus_tag="VIX62_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5859324.1"
                     /translation="MTTDELFSVRDKVVLVTGGSRGIGEMIAAGFLANGAKVYISSRK
                     AEVCDATAARLADTYGGECISIPADLAALDGIDHLGTEVSARESRLDVLVNNAGVSWG
                     ASIDEFPEKGWDKVMDTNVKGVFFLTQRLLPLLEAEATADDPARVINIGSVDGIRTPS
                     FDTVSYGPSKAAVHALTSQLAARLVKRNILVNAIAPGPFPTWMLSTGVGTGGDVEGTD
                     WDALGRSMGRGRIGTPEDIAGLAIFLASRAGAFTVGAVITCDGGLVVS"
     gene            2013..>3213
                     /locus_tag="VIX62_03480"
     CDS             2013..>3213
                     /locus_tag="VIX62_03480"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5859325.1"
                     /translation="MTKAAIANATALLARIGADVRDDEELRQKKALVVLITVLILPVS
                     ALWGVLYLALGSLIGISPLVYFAVSLGSLVVFARTRRFQPFLLTQLVDILVMPTTGQM
                     LLGGFLASGGVGLWGILAPLGALVFREVRQAVRWFIAFVVVFLVTGLAGEIFVSNADL
                     PVSFTTTMLALNVIGAASISFTLLALFARERNEALEALRAEQEKSELLVLNILPRPIA
                     ERLKVTAEPIADHLPAVSIVFADVVDFTPLAQRLPPAEMIRLLDHLFSDIDMLVERHG
                     LEKIKTIGDCYMAAAGVPNQHPDHAHKAALLALEMREVLATSAVDDQPGLELRIGINS
                     GPVVAGVIGKKRFLYDLWGDAVNTASRMESHGTPGEIQITRATYELVKDEFECRPRGT
                     IDVKGKGP"
BASE COUNT          449 a         1117 c         1078 g          569 t
ORIGIN      
        1 caccgacctg accgaccgca tctcggatcg gctcccggtg ttcatcgccg ccgtcgtggc
       61 gctgtcgttc ctcctgctga tgatcgtgtt ccggtcggtc ctggtcccgc tcaaggcggc
      121 ggtcatgaac ctgctctcga tcgccgccgc ctacggcgtg ctcgtggccg tgttccagtg
      181 gggctggggc gccgagctca tcgggctgca cagcacgatg ccgatcaacc cgttcctccc
      241 gctcatcatg ttcgccatcc tcttcgggct ctcgatggac tacgaggtgt tcctcctgtc
      301 ccgggtgcgt gaggagtacg tgaagaccgg cgacaaccac gaagccgtgg tgcgggggct
      361 gtccagcacc gctcgggtga tcacctcggc cgccttgatc atgatcagcg tgttcggcgc
      421 cttcgtcctc agtgacgacc cgaacggcaa gctcttcggg gtcggcctgg ccgtcgccgt
      481 cctcatcgac gccaccctcg tgcgcatggt cctggtgccg gcgaccatga gcctcctggg
      541 ccgggccaac tggtggctcc ccagctggct cgaccgggtc ctcccgcacc tcgacctcga
      601 gggcggcggt cccaccgttc ccgaggtcag ccccgagccg gagcccgagc ccgagcgcga
      661 gctcgccccc gtctgatcag gcgtacaccg tgcgggtgag ccaatcggcc accagggccg
      721 gcttctcctc gccctcgatc tcgatggtga agctccgggt gacctcgacg gcattgccct
      781 tgagctcggc gttcacggtc accgcccggg cccggatgcg ggatccgacc ttcacggggc
      841 tcacgaaccg gaccttgttg aagccgtagt tgatgcccat catgacgccc tcgtacttcg
      901 ccgggtcggg cggggcggcg ctggccccgg cggcgaggtg ggtgagcatc gagagggtga
      961 ggaagcgtgg gcgatcgtgg tgccgaacgg cgtggctgct gctcgctccg ggtcgacgtg
     1021 gatgaactgg tggtcgtggg tgatgtcggc gaagacgttc acctggtcct gggtgacgtc
     1081 gaaccactcg ccggtgcctt cgtcgttgcc gacgtcggcc tggaacagcg ggaacgcctt
     1141 ggctgctgct tcactcatgg cgctgcaccc tagggggtgc tccgcaacga tcgaagggga
     1201 cccatcgtga ccagcctgac caccgacgag ctgttctccg tccgagacaa ggtggtgctc
     1261 gtgaccggtg ggtcgcgcgg catcggcgag atgatcgcgg ccgggttcct ggcgaacggc
     1321 gccaaggtct acatcagctc gcggaaggcg gaggtctgcg acgccaccgc ggctcgcctc
     1381 gccgacacct atggcggcga gtgcatctcc atccccgctg acctggcggc gctcgacggc
     1441 atcgaccacc tgggcacgga ggtgtcggcg cgcgagtcgc ggctcgacgt gctggtgaac
     1501 aacgccgggg tctcctgggg cgcgtccatc gacgagttcc cggagaaggg ctgggacaag
     1561 gtcatggaca cgaacgtcaa gggcgtcttc ttcctcaccc agcggctgct gcccctgctc
     1621 gaggccgagg cgacggcaga cgaccctgcc cgcgtgatca acatcggctc ggtcgacggg
     1681 atccggacac cgtcgttcga caccgtgtcg tacgggccgt cgaaggccgc ggtgcacgcg
     1741 ctcacgagcc agctcgccgc ccgcttggtc aagcggaaca tccttgtgaa cgcgatcgcc
     1801 ccggggccgt tcccgacctg gatgctgagc accggtgtcg ggaccggagg cgacgtggaa
     1861 ggcaccgact gggacgcgct cggccgatcg atgggccgcg gccgcatcgg gacgccggag
     1921 gacatcgccg gcctggcgat cttcctcgcc tcgagggccg gggcgttcac cgtcggcgcg
     1981 gtcatcacct gcgatggtgg cctggtcgtg tcgtgaccaa ggcggcgatc gccaacgcga
     2041 ccgcgttgct cgcgcggatc ggtgccgacg tccgggacga cgaggagctg cgccagaaga
     2101 aggcgctcgt cgtcctgatc acggtgctca tcctgcccgt gtccgccctg tggggcgttc
     2161 tctacctcgc cctcggctcg ctgataggca tctctccgct cgtctacttc gcggtgtcgc
     2221 tcggttcgct ggtggtcttc gcgcggacgc gccgcttcca gccgttcctg ctcacccagc
     2281 tcgtcgacat cctcgtgatg ccgaccaccg ggcagatgct gctcggcggg ttcctggcct
     2341 ccggcggggt cgggttgtgg ggcatcctgg cgccgctcgg cgccctcgtc ttccgagagg
     2401 tgcgtcaggc tgtccgctgg ttcatcgcgt tcgtcgtggt cttcctggtc acgggcctcg
     2461 cgggcgagat cttcgtctcg aacgcggacc tcccggtctc gttcaccacc acgatgctgg
     2521 cgctgaacgt catcggtgcc gcctccatct cgttcacgct cctcgcgctg ttcgcccgcg
     2581 agcgcaacga agcgctcgag gcccttcgag ccgagcagga gaagtccgag ctcctcgtcc
     2641 tgaacatcct tccgcgcccg atcgccgagc gactgaaggt caccgccgag ccgatcgccg
     2701 accacctccc tgcggtgtcc atcgtcttcg ccgatgtcgt cgacttcacc ccgctcgcgc
     2761 agcgcctccc cccggccgag atgatccgcc tcctcgatca cctcttctcc gacatcgaca
     2821 tgctcgtgga gcggcacggg ctcgagaaga tcaagacgat cggcgattgc tacatggccg
     2881 ctgcgggcgt gccgaaccag catccggacc acgcgcacaa ggccgcactc ctggcgctcg
     2941 agatgcgcga ggtgcttgcg acctcggcgg tggacgacca gcccggtctc gagctgagga
     3001 tcggcatcaa ctctgggccg gtcgtcgccg gggtgatcgg caagaagcgc ttcctctacg
     3061 acctgtgggg cgacgccgtg aacacggcga gtcggatgga gtcccacggc acccccggtg
     3121 agatccagat cacgcgcgcg acctacgagc tcgtgaagga cgagttcgaa tgccggccgc
     3181 ggggcacgat cgatgtcaag ggcaaggggc cga
//
LOCUS       DATDBZ010000142         3165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_256688, whole genome shotgun sequence.
ACCESSION   DATDBZ010000142 DATDBZ010000000
VERSION     DATDBZ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256688"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VIX62_03485"
     CDS             complement(<1..500)
                     /locus_tag="VIX62_03485"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY5859326.1"
                     /translation="MPFWKRRPARRLPADWTRLMRTSLGEWTYLNEDERQRLGELIAG
                     LLAGKSWEAARAFTLTDEMCTLIAAQAALMVLGLDLDAYREVRAVIVHPTTVTSRRPR
                     PGPVAGVVTEEPLDLLGEAHQQRGPVLVAWDTAAGDARHAGTGRNVVIHEFAHKLDML
                     DGMVDGT"
     gene            complement(572..2059)
                     /locus_tag="VIX62_03490"
     CDS             complement(572..2059)
                     /locus_tag="VIX62_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY5859327.1"
                     /translation="MSELGRDVIAPASATPRSGAVFVITALGTFMASLDLSIVNVAFP
                     DIEASYPDASSASLAWIITAYSIVFGALLVVGGRTGDRLGRKRVFLGGVVVFLLGSFL
                     CGIAPNVATLVGARALQGIGAAFLVPASVALLIGAYRPERRMQIVAQWGGIGALAVAT
                     GPSLGAAIVSTGGWRWAFFVNIPVGLFILLFGRRVLTESVGDSDPKRPDYLGVALMSL
                     ALASLVLAISEGSTWGWTDARILSAFAVALVTGAVFAHRTFHHDDPVIDPALFHSRPF
                     VIANTATIVYAMGFFAMLLGNILFLTGVWNYSIMQAGLAVTPGPLVVAIIAGPAGKLA
                     ARVGFRPVLLFGASCFAAGLVSYIVLVTTTPSYLATWLPGTLLVGVGVGFTFPVLSAG
                     AVSSLPPARFAVGSAVNQTCRQIGGALGIAVLVALIGSQRQLADPLDGFHQLWAFAAT
                     TALVSGIIGSFIPRPTSHQSTDMDELADELLVIEADGLPLAVDGA"
     gene            2115..2609
                     /locus_tag="VIX62_03495"
     CDS             2115..2609
                     /locus_tag="VIX62_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5859328.1"
                     /translation="MKRTSFETMNCSVAQCLEVVGEWWSMLIVRDAFLGVRRFDDFQA
                     RLGISRNILNVRLTSLVEAGVLVRVRYQERPERHEYRLTDKGRDLWPVLTTMREWGDK
                     WAAPNGAPLEVVHDSCGGVMHLKPTCSACGEPVDGRSVRVRAGPGATPGDFDRTVLAA
                     PTGW"
     gene            2623..2696
                     /locus_tag="VIX62_03500"
     tRNA            2623..2696
                     /locus_tag="VIX62_03500"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2657..2659,aa:Val,seq:gac)
     gene            complement(2767..>3165)
                     /locus_tag="VIX62_03505"
     CDS             complement(2767..>3165)
                     /locus_tag="VIX62_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5859329.1"
                     /translation="DAFLGVRRFDDFQARLGISRNILNVRLTSLVEAGVLVRVRYQER
                     PERHEYRLTDKGRDLWPVLTTMREWGDKWAAPNGAPLEVVHDSCGRVLHLKPTCSACG
                     EPVDGRSVRVSAGPGATPGDFDRTALEAPT"
BASE COUNT          555 a         1071 c         1094 g          445 t
ORIGIN      
        1 gtgccgtcga ccatcccgtc gagcatgtcg agcttgtggg cgaactcgtg gatcacgacg
       61 ttgcggcccg tgccggcgtg ccgggcgtcg ccggccgcgg tgtcccaggc caccagcacc
      121 ggcccccgct gctggtgcgc ctcgcccagc aggtcgagag gctcctcggt gaccaccccc
      181 gccacggggc cgggacgcgg gcgccgtgat gtgaccgtcg tcgggtgcac gatgacggca
      241 cgcacctcgc gatacgcatc cagatccagc ccgagcacca tgagggcagc ctgcgcggcg
      301 atgagcgtgc acatctcgtc cgtcaacgtg aaggcgcgag ccgcctccca gctcttcccg
      361 gccaggagcc cagcgatgag ctcacccagc cgttgccgtt cgtcctcgtt caagtaggtc
      421 cactcgccga gcgacgtgcg catcagccgg gtccagtccg ccggcagccg gcgcgccggg
      481 cgtcgcttcc agaacggcac gccgacaggg tggcacgcgc gccccctggc agcactgacg
      541 caggtggtcc cgatgaaccg cccgtcgccc gtcacgcacc gtccacggcg agcgggagcc
      601 cgtcggcctc gatgacgagc aactcgtccg cgagctcgtc catgtctgtc gactggtgtg
      661 acgtgggccg ggggatgaac gacccgatga tgccggagac gagtgcggtg gtggcggcga
      721 acgcccacag ctggtggaac ccgtcgaggg ggtcggcgag ctgacgttgg gatccgatga
      781 gcgccacgag caccgcgatg ccgagagcgc cgccgatctg acgacaggtc tgattgaccg
      841 cgctccccac ggcgaaacga gcgggcggca acgacgagac cgcaccggcg ctcagcaccg
      901 ggaacgtgaa cccgacgccg acgccgacga gcaacgtccc gggcagccag gtcgccagat
      961 agctcggggt cgtggtgacc aggacgatgt agctcacgag gccggccgcg aagcacgatg
     1021 cgccgaacag cagcacgggt ctgaacccga cgcgtgctgc caacttgcca gctggcccgg
     1081 cgatgatcgc gacgacgagc ggccccggcg tcacggcgag accggcctgc atgatcgagt
     1141 agttccagac tccggtgagg aacaggatgt tgccgagcag catggcgaag aagcccatgg
     1201 cgtacacgat ggtcgcggtg ttggcgatga cgaacgggcg actgtggaac agggcggggt
     1261 cgatgaccgg gtcgtcgtga tggaaggtgc gatgcgcgaa caccgcgccg gtgacgagcg
     1321 ccaccgcgaa cgcgctgagg atccgcgcgt cggtccagcc ccacgtcgac ccttccgaga
     1381 tggcgagcac caaggacgcc agcgccagcg acatgagtgc gacgccgagg tagtcgggcc
     1441 tcttcgggtc cgaatcgccg accgattcgg tcagtacccg ccgtccgaac agcaagatga
     1501 acaggccgac ggggatgttc acgaagaatg cccatcgcca cccgccggtc gacacgatgg
     1561 ccgcacccag cgaggggccg gtggcaaccg ccaaggcgcc gataccgccc cactgggcga
     1621 cgatctgcat gcgtcgctcg ggtcggtagg cgccgatcag caatgccacc gaggccggca
     1681 ccaggaacgc ggcgccgatc ccctggagcg cacgagcccc gaccagtgtc gcgacgttgg
     1741 gtgcgatgcc acagaggaac gagcccagta ggaacaccac gacaccgccg aggaagacac
     1801 gcttgcggcc gagccgatca ccggtgcgtc cgccgacgac cagcagcgcg ccgaacacga
     1861 tggagtacgc agtgatgatc catgccagcg acgccgagct cgcatctgga taggacgcct
     1921 cgatgtcagg gaacgcgacg ttcacgatcg acaagtcgag cgatgccatg aacgtgccca
     1981 aggccgtgat gacgaacacc gcacccgatc ggggcgttgc gctcgccgga gcgatgacgt
     2041 cacggccgag ttcgctcatc gaacgcaccc taagcgattg cgttctatca tgcaacccac
     2101 taccgtgcgg gggcatgaag cgcaccagct tcgagacgat gaactgctcc gtcgcgcaat
     2161 gcctcgaggt cgtcggggag tggtggtcga tgctgatcgt ccgcgacgcg ttcctcggtg
     2221 tacgccgctt cgacgacttc caggcacggc tgggcatctc ccgaaacatc ctcaacgtgc
     2281 gactgaccag cctcgtcgag gctggcgtcc tggtgcgggt gcgctatcag gagcgccccg
     2341 agcgccacga gtaccggctc acggataagg gacgcgacct ctggccggtc ctcaccacga
     2401 tgcgggagtg gggcgacaag tgggcggctc ccaacggcgc tccgctcgag gtcgtgcacg
     2461 actcgtgcgg cggggtcatg catctgaagc cgacgtgcag cgcctgcggt gaacccgtcg
     2521 acggacggtc agttcgcgtc agggctggcc ccggcgcaac gcctggtgac ttcgaccgca
     2581 ccgtgctggc ggcgccgacc ggctggtagc gttcgcggct ccgggcgcgt agctcagttg
     2641 gttagagcgc caccttgaca cggtggaggt cacacgttcg agtcgtgccg tgcccacgga
     2701 gaaggttctg gccacaggcc ttctgcctcg gacagttcgc gttgacgaac ggtcaggttg
     2761 cgcagctcat gtcggcgcct ccagcgcggt ccggtcgaag tcaccaggcg ttgcgccggg
     2821 gccagcgctg acgcgaactg agcgtccgtc cacgggttca ccgcaggcgc tgcacgtcgg
     2881 cttcagatgc aggacccggc cgcacgagtc gtgcacgacc tcgagcggag cgccgttggg
     2941 cgccgcccac ttgtcgcccc actcccgcat cgtggtgagg accggccaga ggtcgcgtcc
     3001 cttatccgtg agccggtact cgtggcgctc ggggcgctcc tgatagcgca cccgcacgag
     3061 gacgccagcc tcgacgaggc tggtcagtcg cacgttgagg atgtttcggg agatgcccag
     3121 ccgtgcctgg aagtcgtcga agcggcgtac accgaggaac gcgtc
//
LOCUS       DATDBZ010000143         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_662737, whole genome shotgun sequence.
ACCESSION   DATDBZ010000143 DATDBZ010000000
VERSION     DATDBZ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662737"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VIX62_03510"
     CDS             complement(<1..979)
                     /locus_tag="VIX62_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5859330.1"
                     /translation="MGIGVYELSASPMLSAFDEQDSRGIRVASEPDHWRGTEPLRVLA
                     PDGTAVGDTDVGLDPDELRATLRWMQLARRLDRECIALQRQGELTVYPGFEGQEAAQI
                     GSAMALGPDDFVFPTFRELAAALVRGVDPVQYLAYHRGTWHGGPYDPRATRFGPICIP
                     VATQIVHAVGYAMGLALDGTRACSLAYFGDGSASEGDFHEAANLAGVFTAPAILFCQN
                     NGWAISVPTERQTAGEIFKRAEGYGFPGVRVDGNDVLAVYRATREAAARAYAGEGPTL
                     IEALTYRIGAHSTADDPGRYRDDDEVSAARAFDPIVRFRSWLGGQGHVDD"
     gene            978..1784
                     /locus_tag="VIX62_03515"
     CDS             978..1784
                     /locus_tag="VIX62_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859331.1"
                     /translation="MVPSAFLRVYQPLDAFERSEQLHWERFLLHGPETMSLRPRYADH
                     RTVGTLGVLAPAEADTAEVLHVDGRTLVSPRRMRMRVLAAMVSFRQSQPIELWEQFVP
                     KKDGKRAAKDLARLRRRDPSAVSFCHQSPWHVPIRWFVLFRDEERELIEDAQGRWRLH
                     YRTTTRRAIRRAEQAIGPLRRSDLGPIGDLIIDLHQWMVQFDPTSVIELDYASLCDFQ
                     RWDELDDDHSAREVHEALQALESGEYPKAADVYQGVLTRWAEVRSREILN"
     gene            complement(1802..1874)
                     /locus_tag="VIX62_03520"
     tRNA            complement(1802..1874)
                     /locus_tag="VIX62_03520"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1839..1841),aa:Phe,seq:gaa)
BASE COUNT          344 a          744 c          780 g          336 t
ORIGIN      
        1 cgtcgtcgac atggccctgg cccccgagcc acgagcggaa ccgcacgatc gggtcgaacg
       61 cgcgcgccgc cgagacctcg tcgtcgtcgc ggtagcgccc cgggtcgtcg gccgtcgagt
      121 gcgcgccgat gcggtacgtc aaggcttcga tcaaggtcgg cccctcgccg gcgtatgccc
      181 gcgccgctgc ctcacgcgtg gcccggtaca cggcgagcac atcgttgccg tcgacccgga
      241 cccccgggaa gccgtagccc tcggcgcgct tgaagatctc gccggcggtc tgccgctcgg
      301 tcggcacgct gatcgcccag ccgttgttct gacagaagag gatcgccggg gctgtgaaga
      361 cgccggcgag gttcgccgcc tcgtggaagt cgccctcgct cgcgctgccg tcgccgaagt
      421 aggcgaggga gcacgcccgc gtgccgtcga gcgccaagcc catcgcgtag ccgacggcgt
      481 gcacgatctg ggtcgcgacc gggatgcaga tcggcccgaa ccgagtggca cgcgggtcgt
      541 acggaccccc gtgccaggtt ccccggtggt aggcgaggta ctgcaccgga tcgacgccgc
      601 gcacgagcgc cgccgcgagc tcgcggaacg tcgggaagac gaagtcgtcg ggcccgagcg
      661 ccatcgcgct gccgatctgg gcggcttcct ggccctcgaa accggggtag accgtcagct
      721 caccctggcg ctgcagggcg atgcattcgc ggtcgagccg gcgggcaagc tgcatccagc
      781 gcagcgtcgc tcgaagttcg tcgggatcga gccccacgtc ggtgtccccc acggccgtgc
      841 cgtcgggggc gagcacgcgg agcggttccg tgccgcgcca atgatctggt tcggacgcca
      901 cgcggatccc cctcgagtcc tgctcatcga acgccgagag cataggggaa gccgacagct
      961 cgtagactcc gatgcccatg gtgcccagcg cgttcctccg tgtgtaccag ccgctcgacg
     1021 cgttcgagcg gagtgaacag ctgcattggg agcgcttcct gctgcacggc cccgagacga
     1081 tgtctcttcg cccccggtac gccgaccacc gcacggtcgg cacgctcggg gtgctggcgc
     1141 cggccgaggc agacacggcc gaggtgctcc acgtcgacgg acgcacgctg gtcagccccc
     1201 ggcgcatgcg catgcgggtg ctcgccgcga tggtgtcgtt ccgccagtcc cagccgatcg
     1261 agctctggga acagttcgtg ccgaagaagg acggcaagcg ggccgcgaag gatctcgcgc
     1321 gtctgcgccg gcgcgatccc tcggcggtgt cgttctgtca ccagagcccg tggcacgtgc
     1381 cgatccgctg gttcgtgctc ttccgcgacg aggaacgcga actgatcgag gatgcgcagg
     1441 gccggtggcg actgcactac cgcacgacca cacgtcgcgc gatccgccgg gcggagcagg
     1501 cgatcggacc gctccgtcgc agcgacctgg gtccgatcgg cgacctgatc atcgacctgc
     1561 accagtggat ggtgcagttc gatccgacgt cggtgatcga gctcgactac gcgtcgttgt
     1621 gcgacttcca gcggtgggac gagctggacg acgatcactc ggcgcgcgag gtgcacgagg
     1681 ccctgcaggc gttggaatcg ggcgagtacc cgaaggcggc cgatgtgtac cagggcgtgc
     1741 tcaccaggtg ggccgaggtc cgaagccgcg agatcctgaa ctgagcagcc ctcgtcgtcg
     1801 gtggccaggg gcagaatcga actgccgaca ccgcggtttt cagccgcgtg ctctgccaac
     1861 tgagctacct ggcccgggcc ggtagagaac ctggcagaaa tcgataccca ggtcatcacc
     1921 agcgcgttga agtgtagcga gtagaggtga gtcggggctc ggtaacagca actctgtctg
     1981 agggatgtgt ccgagccagc gttgatcgct gccgggtggt gccggaggcg gaggcagtca
     2041 ggcggccgct tcgagaccct cagccgctct gtggcgggcc atcgtgaggg cgttgaccat
     2101 cacagcccag ccgagcatct cgacctgctg gcggcgccac ccgagactgt gagctctgcc
     2161 gaggaacagg gtgtcctcca gggaccggtt gagcgattcg gagt
//
LOCUS       DATDBZ010000144         2192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_869518, whole genome shotgun sequence.
ACCESSION   DATDBZ010000144 DATDBZ010000000
VERSION     DATDBZ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869518"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VIX62_03525"
     CDS             <1..247
                     /locus_tag="VIX62_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859332.1"
                     /translation="LTWFRWHPWKAVGGGLFHLNDCHPSCAEGTFHKAWGHLWLRNRA
                     RCDPPGAFVFQHVRVVYEGRLLGRHRTAFGHLGCPFR"
     gene            complement(258..1829)
                     /locus_tag="VIX62_03530"
     CDS             complement(258..1829)
                     /locus_tag="VIX62_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5859333.1"
                     /translation="MSTGVSHPEETLPHKELGPLPRPLRPFVDWVARIKATVHTKLLA
                     GFMLIALLLLSMGVVSISVLNRVNDQVETLTRLNHQASQARDMIYGVTAQSHYRAMAL
                     LKLDDPTWTPKIYAAKDEFAKNMAEMRTYAVPSEVALLDHVESTNEVYAESSQAVTDL
                     FEVGKVDQALQLHIEQEHEVSHVLEAQLNELIADSQALVTQETDGFAENRRFLTIAVG
                     AFSGVSLLIALTLGAILSWSLIRPVRSVDVALEQIAGGDFNTHVEVPNRDEFGNLTRN
                     LNRTTGQLSALYQRLHDLNANLQETVETKVAELERTSRLRRYLSPQLADSIVSGEQDV
                     ALEPSRKYLTTFFSDVRGFTAAAERMDPGQLVHEMNDYFAAMTEIVFKHGGTLDKYVG
                     DAVMVFFGDPIPQEDHAARAVRMGIEMLERMKTLSDHWQQQYGEVFEIGIGISTGWVT
                     VGDLGSPARSDYTVLGDQVNVASRLADRAEARRILVTEETMTAVEGIVEGRLVGEVRL
                     KGITRQVKVYELVVP"
     gene            complement(1917..>2192)
                     /locus_tag="VIX62_03535"
     CDS             complement(1917..>2192)
                     /locus_tag="VIX62_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5859334.1"
                     /translation="TPKAGTFAEGAALVVANQLIARVRGEAAPPGFDGTGACWIEFGD
                     EQVGRVDVDFFSTPGSPIGTFVPPSAEVAAEKASFAGTRRERWFGTA"
BASE COUNT          327 a          728 c          738 g          399 t
ORIGIN      
        1 cctcacgtgg ttccgttggc atccgtggaa ggcggtgggc ggcgggctgt tccatctgaa
       61 cgactgccat ccgagctgcg cggaagggac gttccacaaa gcgtggggcc atctgtggct
      121 acggaaccgc gcgcggtgtg atccaccggg cgcgttcgtg ttccagcacg tccgggtcgt
      181 ctacgaaggg cgattgctgg gccggcaccg gaccgcgttc ggccatctcg ggtgtccgtt
      241 ccgctgagct ccttccctta cgggacgacc agctcgtaga ccttcacctg gcgggtgatg
      301 cccttcagtc tgacctcgcc gaccaaccgg ccctcgacga tgccctcgac ggccgtcatc
      361 gtctcctcgg tgaccaggat ccggcgcgcc tcggcgcggt cggcgagccg agaggcaacg
      421 ttcacctggt cgcccaggac ggtgtagtcg ctccgcgccg gtgaacccag gtcgcccacg
      481 gtgacccacc ccgtcgagat gccgatcccg atctcgaaca cctcgccgta ctgctgctgc
      541 cagtgatcgg acagcgtctt catgcgctcg agcatctcga tccccatcct gaccgcccgc
      601 gccgcgtggt cctcctgagg gatcgggtcg ccgaagaaca ccatgaccgc gtcgcccacg
      661 tacttgtcga gcgtgccgcc gtgcttgaac acgatctcgg tcatggccgc gaagtagtcg
      721 ttcatctcgt gcaccagctg ccccgggtcc atgcgttcgg ccgcggcggt gaacccccgc
      781 acgtcggaga agaacgtggt gaggtactta cggctcggct ccagcgcgac gtcttgttca
      841 ccgctgacga tcgaatcggc gagctggggc gagaggtagc gacgcagccg tgaggtgcgt
      901 tcgagctcgg ccaccttcgt ctcgacggtt tcctgcaggt tggcgttgag gtcgtgcagc
      961 cgttggtaga gggcggacag ctggccggtc gtgcggttga ggttcctggt gaggttgccg
     1021 aactcgtcac ggttggggac ctcgacgtgg gtgttgaagt cgccgcccgc gatctgctcc
     1081 agggccacgt cgacgctgcg caccggccgg atcagcgacc aggacaggat cgcgccgagc
     1141 gtcagggcga tcagcaagct gacccccgag aaggccccga ccgcgatcgt gaggaaccgg
     1201 cggttctcgg cgaagccgtc cgtctcctgt gtcacgaggg cttgcgagtc ggcgatcaac
     1261 tcgttgagct gcgcctcgag cacgtgggag acctcgtgct cctgttcgat gtggagctgc
     1321 aacgcctgat cgaccttgcc cacctcgaac aggtccgtca cggcctggct ggattcggcg
     1381 tagacctcat tcgtggattc gacgtgatcg agcagcgcga cctcgctcgg caccgcgtag
     1441 gtgcgcatct cggccatgtt cttcgcgaac tcgtccttcg ccgcgtagat cttcggcgtc
     1501 caggtggggt cgtcgagctt cagcagcgcc atggcccggt aatggctctg cgccgtgacg
     1561 ccgtagatca tgtcgcgcgc ctggctcgcc tggtggttga gacgggtcag cgtctccacc
     1621 tggtcgttga cgcggttcaa cacgctgatg ctcacgacgc ccatcgacag cagcagcagc
     1681 gcgatcagca tgaagccggc cagcagcttg gtgtggacgg tggccttgat gcgtgcgacc
     1741 cagtccacga acggccgaag cggccgtggc agcgggccga gttccttgtg cggcagtgtc
     1801 tcctcgggat gtgacacgcc cgtcgacatg gtggatcccc ctcgatgtcc gatggtgtgt
     1861 cggaccagga tggaccattc gtgacgagtc gtgaagggcg ggctcgcggg ggtcgctcag
     1921 gccgtgccga accatcgttc gcggcgagta cccgcgaacg aggccttctc ggccgccacc
     1981 tcggccgagg gcggcacgaa cgtgccgatc ggggagccgg gggtcgagaa gaagtccacg
     2041 tcgacgcgcc cgacctgctc gtcgccgaac tcgatccagc atgcgccggt gccgtcaaag
     2101 ccgggtggtg ctgcctcgcc ccgcacgcgc gcgatcagct ggttcgccac cacgagcgcg
     2161 gctccttcgg cgaacgtccc cgccttcggc gt
//
LOCUS       DATDBZ010000145         2453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1890688, whole genome shotgun sequence.
ACCESSION   DATDBZ010000145 DATDBZ010000000
VERSION     DATDBZ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890688"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VIX62_03540"
     CDS             <1..578
                     /locus_tag="VIX62_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5859335.1"
                     /translation="MNKRNTFDDFLACADHLVTLGYAAPDRVAIRGGSAGGLLVGVAM
                     TLRPDSINTVIAEGPFVDVISSMSDPSLPLTITEWEEWGDPRQEPAASYMLAYSPYDN
                     VAARAYPALYVTAGLNDPRVSFHEPAKWVAKLRAVGTGSRPLLLRTEMGAGHGGPSGR
                     YDAWRDEAQVLAFLLAVMGHDIDTGVTARSR"
     gene            complement(559..909)
                     /locus_tag="VIX62_03545"
     CDS             complement(559..909)
                     /locus_tag="VIX62_03545"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY5859336.1"
                     /translation="MLLGVAMLVAGGGKLASPSWPEHARGLGAPAWAVPVVPWIEIGL
                     GALLVVQWSRPFVAAVTAALLLVFSVLLVRRLAEGHRPPCACFGGFSTRPIGPWSVVR
                     NTVLIGLAIIAIVR"
     gene            complement(940..1761)
                     /locus_tag="VIX62_03550"
     CDS             complement(940..1761)
                     /locus_tag="VIX62_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5859337.1"
                     /translation="MRGSTPCHGDDIKRYGGNTSCVSVALPGEAPIVFDLGTGLRYFG
                     KTQPLDGSFRGSCLLSHLHWDHTQGLPFFGPILLQGSHLDVYAPIQEDGRPSAEAIRA
                     FMRPPHFPISLDDLPGTVDFHDVGDTEFEIGGARVLSRLLPHVGNTLGFRLTFAGTSI
                     VYISDHQQPYDGSHAMSDGARELAQSADLLIHDSQYTMDEFRVKRNWGHCTVEYALWL
                     AAECDVKRLALFHHDPSRTDDSIDELAACATLAGRLSDVEVFAASEGLTVEIPIA"
     gene            1887..>2453
                     /locus_tag="VIX62_03555"
     CDS             1887..>2453
                     /locus_tag="VIX62_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5859338.1"
                     /translation="MPGGITQGAVRANGFEVAYLECGSGPLALCLHGFPDSAHTWRHL
                     LPALAGAGFRAVAPFQRGYAPSEVPKDGRYQTGALALDAIMLHEVLGGDGYAAIIGHD
                     WGAPATYGAASHEPERWSKVVGMAVPPGAAVAQAFLTNLEQLKRSWYMFFFQHPLADL
                     VVPANDLAFIDRLWRDWSPGFDASEELPL"
BASE COUNT          402 a          843 c          837 g          371 t
ORIGIN      
        1 tgatgaacaa gcgcaacacc ttcgacgact tcctcgcgtg tgccgaccat ctcgtcaccc
       61 tcggctatgc ggcgcccgac cgcgtggcga tccgcggcgg ctcggcgggc gggctgctcg
      121 tgggcgtcgc catgacgctt cggccggatt cgatcaacac ggtgatcgca gagggtccct
      181 tcgtcgacgt catctcctcg atgagcgacc cgagcctgcc gctcaccatc accgagtggg
      241 aggagtgggg cgacccgcgc caggagccgg cggcgagcta catgctcgcc tactcgccct
      301 acgacaacgt cgccgcccgc gcctacccgg cgctgtacgt caccgccggc ctgaacgacc
      361 cacgggtctc cttccacgag ccggcgaagt gggtggcgaa gctgcgcgcc gtcggtaccg
      421 gatcgcgccc actgctgctc cggaccgaga tgggggcggg ccacggcgga ccgtccggtc
      481 gctacgacgc gtggcgggac gaggcgcaag tcctcgcctt cctgctcgcc gttatgggcc
      541 acgacatcga cacgggtgtc accgcacgat cgcgatgatc gccaggccga tgaggacggt
      601 gttgcgtacg acgctccatg gtccgatcgg tcgcgtcgag aagccgccga agcaggcaca
      661 cggagggcga tgaccctccg ccagccggcg cacgagcagc acgctgaaca cgagcaacaa
      721 cgccgccgtc accgccgcca cgaacggcct cgaccactgg acgacgagca gcgcgcccag
      781 gccgatctcg atccacggca cgaccggcac cgcccacgcc ggcgcgccga gtccgcgagc
      841 gtgctcgggc caggaggggc tggcgagctt gccgccaccg gcgacgagca tcgcgacgcc
      901 gaggagcacg gccgcagcct ccgctatgcc cactcgtcgt caggcgatcg ggatctcgac
      961 cgtcagaccc tcggaggcgg cgaacacctc gacgtcgctg agccggccgg cgagcgtcgc
     1021 gcaggccgcc aactcgtcga tcgagtcgtc ggtacggctg ggatcgtggt ggaacagcgc
     1081 cagtcgcttc acgtcgcact cggccgccag ccacagcgcg tactcgaccg tgcaatggcc
     1141 ccagttccgc ttcacgcgga actcatccat cgtgtactgc gagtcgtgga tgagcagatc
     1201 agccgactgc gcgagttccc gagcaccgtc ggacatcgcg tgcgagccgt cgtagggctg
     1261 ttggtgatcg gagatgtaga cgatcgacgt gcccgcgaac gtgaggcgga agccgagcgt
     1321 gttgccgacg tgcggcagca ggcgagacag gacgcgggca ccgccgatct cgaactccgt
     1381 gtcgccgacg tcgtggaagt ccaccgtgcc cgggaggtcg tcgagcgaga tcgggaagtg
     1441 tggtggccgc atgaaggcgc ggatcgcctc ggccgacggc cgaccgtcct cctggatcgg
     1501 tgcgtacacg tcgaggtgcg acccctgcag caggatcgga ccgaagaacg gcagcccctg
     1561 tgtgtgatcc cagtgcaagt gactgagcag gcacgagccc cggaacgacc cgtcgagcgg
     1621 ctgtgtcttg ccgaagtaac gaagaccggt gcccagatcg aacacgatcg gtgcttcgcc
     1681 gggcagggcg accgagacgc acgacgtgtt cccgccgtat cgcttgatgt catcgccgtg
     1741 gcatggcgtg gaaccgcgca caccgtggaa cgtgactgag atcgccgtcg agcccccgat
     1801 gagcagggca cgctaaacac gctggaccac cgtgtccaga cgcgggactg tgatgttggt
     1861 cgctccggcg gagttatggt catcgtgtgc ctgggggaat cacgcagggc gccgtgcgcg
     1921 ccaacggctt cgaggtcgcg tacctcgagt gtggatcggg tcccttggcg ctctgcctgc
     1981 acggattccc cgactccgcc cacacgtggc gacacttgtt gcccgccctt gccggtgccg
     2041 gcttccgcgc cgttgctccc ttccaacgtg gttacgcgcc gagcgaggta ccgaaggacg
     2101 gtcgatacca gacgggagcg ctggcgctgg acgcgatcat gctgcacgag gtcctgggcg
     2161 gtgacggcta cgccgccatc atcggccacg actggggtgc tccggccacc tacggagcgg
     2221 cgagccacga gcccgagcga tggtcgaagg tcgtcgggat ggcggtgccg cccggcgcgg
     2281 cggtggcgca ggcgttcctg accaatctcg agcagctgaa gcgatcctgg tacatgttct
     2341 tcttccagca cccgctggcc gatctcgtgg tgccggccaa cgatctcgcc ttcatcgatc
     2401 ggctctggcg tgactggtca cccgggttcg acgccagcga ggagctcccg ctc
//
LOCUS       DATDBZ010000146         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1689054, whole genome shotgun sequence.
ACCESSION   DATDBZ010000146 DATDBZ010000000
VERSION     DATDBZ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689054"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VIX62_03560"
     CDS             complement(<1..905)
                     /locus_tag="VIX62_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY5859339.1"
                     /translation="MAERGSAAAASGFNTSRIWLAAAIVGIVAGGVLQLAGQEDAADI
                     AWAITTVVGIIPMLREVVTGLLRRQPGVDVIALLAMLGALALQEYLAGAVIALMLSTG
                     EALEAYADRRAHRELSALLERAPRTVNRYEGDDLVTRPIEEVAVGDRLMVKTGEVVPV
                     DGVMLGDGVLDESALTGESRPVERPMGDLVRSGAVNAGPAYDLRASTTAEESTYAGIV
                     RLVKEAEQDKAPFVRLADRYATIFIPVTLLIAGAAWLFSGDSTRALTVLMVATPCPLI
                     LAVPIAVVAGISRAARRGIIVKGGGA"
     gene            complement(905..1402)
                     /locus_tag="VIX62_03565"
     CDS             complement(905..1402)
                     /locus_tag="VIX62_03565"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEY5859340.1"
                     /translation="MSVEDVVIGALPDEVGFLAPLLGTWVGEGRGEYPTMEAFDYGER
                     LTFDHVGDAFLQYVHRSWLLEDGTPLHFERGFLRPGAEAGAVELTLAHPLGLTEVSHG
                     TLIDGRMDLVATSMGRTATGMAVVGVARRYRIDGDEIAYELDMATETTPMIRHLTGRV
                     RRVSG"
     gene            complement(1467..1715)
                     /locus_tag="VIX62_03570"
     CDS             complement(1467..1715)
                     /locus_tag="VIX62_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859341.1"
                     /translation="MHGDRDASEAQRALQQAARDRVLSERVDRLEREVARVSAHLDGA
                     SVTLLPSAGSIRPPVGPDPGSALQRIVQGRTDPPDAPR"
     gene            1802..>2147
                     /locus_tag="VIX62_03575"
     CDS             1802..>2147
                     /locus_tag="VIX62_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEY5859342.1"
                     /translation="MRTLARLNVTPVKGLALHHPAQARITEAGLPENRRFYLVDEAGT
                     LFSGSDFGPLVRIVPAYDPAREHLRLTFPDGTIVEGDVSELGAAETSNFYGRTVQSHR
                     VEGPWTEAFSSYI"
BASE COUNT          332 a          789 c          713 g          313 t
ORIGIN      
        1 gcgccgccac ccttgacgat gatcccgcgg cgcgccgcgc gggagatgcc ggccaccacc
       61 gcgatcggca ccgccaggat caacggacac ggcgtcgcca ccatgagcac cgtgagggca
      121 cgggtggaat ccccgctgaa cagccaggcc gcgcccgcga tcagcagcgt gaccgggatg
      181 aagatcgtgg cgtagcggtc ggccagtcgg acgaacggcg ccttgtcctg ttcggcttcc
      241 ttcaccagac gcacgatccc ggcgtaggtg ctctcctcgg ccgtcgtcga tgcccgcagg
      301 tcgtaggcgg ggccggcgtt cacggcgccc gaccgcacga ggtcgcccat cgggcgttcc
      361 accggtcgcg attcgccggt gagcgccgat tcgtcgagga cgccgtcgcc cagcatcacg
      421 ccgtcgacgg gcacgacctc gccggtcttg accatgagcc ggtcccccac cgccacttct
      481 tcgatcggcc tggtgacgag atcgtcgccc tcgtagcgat tgacggttcg cggcgcccgt
      541 tccaacaggg ccgacagctc gcggtgcgct cgccggtccg cgtaggcctc cagagcctcg
      601 ccggtggaga gcatcaaggc gatgacggcg cccgccaggt actcctgcag cgccaaggcc
      661 cccagcatgg ccagcaacgc gattacgtcg acgccgggtt gacggcggag caggccggtg
      721 acgacttctc gcaacatcgg gatgatgccg accacggtgg tgatggccca cgcgatgtcg
      781 gcggcatcct cttggccggc cagctgcagg acgccgcccg ccacgatccc gacgatcgcc
      841 gcggcgagcc agatccgact cgtgttgaac ccgctcgcgg cggccgcgga tccccgctcg
      901 gccatcatcc gctcacccgt cggacgcgac cggtgaggtg acggatcatc ggggtggtct
      961 cggtggccat gtcgagttcg taggcgatct cgtccccgtc gatccggtac cgccgcgcga
     1021 cccccaccac ggccatgccg gtggcggtcc tgcccatcga ggtggctacg agatccatcc
     1081 gtccgtcgat caaggtcccg tggctcacct cggtgagtcc gagcggatgg gccagcgtca
     1141 gttccaccgc cccggcctca gcccccggcc gcaggaaccc gcgctcgaag tgcagcggtg
     1201 ttccgtcctc gaggagccag ctgcggtgca cgtactgcag gaaggcgtcg ccgacgtggt
     1261 cgaacgtcag gcgctcgccg tagtcgaacg cctccatcgt ggggtactcg ccccgaccct
     1321 cacccaccca cgtgcccagc aagggcgcga ggaacccgac ctcgtcggga agggcaccga
     1381 tcacgacatc ctcgacgctc atgtgatgcc tcgaggatgt gcaggagcca tgcagccgaa
     1441 cgtaccacgg tgccccgcgg ccgccctcag cgcggagcgt ccggcggatc ggtgcggccc
     1501 tgcacgatgc gctgcaacgc cgatccgggg tcgggaccca cgggagggcg gatggatccg
     1561 gcactcggca ggagcgtcac cgaagcaccg tccaggtggg cggagaccct cgccacctcg
     1621 cgctcgaggc gatcgacgcg ttcggagagg acccggtcgc gcgccgcctg ctgcagcgct
     1681 cgctgggcct cactcgcatc gcgatcaccg tgcatgcttc gccctcccgg cgtgttcgct
     1741 ccgtcgatcg tacgcccccg aaggacctgg gctcaagcct ggcacaggta tcgtggggtc
     1801 tatgcgaacc ctcgcccggc tcaacgtgac acccgtcaag ggcctcgcgc tgcatcatcc
     1861 cgcgcaagcg cggatcaccg aggccggtct gcccgagaac cgccgcttct acctggtgga
     1921 cgaggcgggg acgctgttca gcggcagcga cttcggtcca ctggtccgga tcgtgccggc
     1981 gtacgacccg gcacgagagc acctgcggct caccttcccc gacggcacga tcgtggaagg
     2041 cgacgtgagc gagctcggcg ccgccgagac ctccaacttc tacggccgca ccgtccagag
     2101 ccatcgggtg gaagggccgt ggacggaggc gttctcctcc tacatca
//
LOCUS       DATDBZ010000147         4522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_257421, whole genome shotgun sequence.
ACCESSION   DATDBZ010000147 DATDBZ010000000
VERSION     DATDBZ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257421"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VIX62_03580"
     CDS             complement(<1..234)
                     /locus_tag="VIX62_03580"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483906.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEY5859343.1"
                     /translation="MAEPLVPKVLVVVCDSWGVGEAPDARAYGDDGSNTLAHTAAVVG
                     GLRVPALEELGLGLLTPIAGVAPRAAAGTAHGRA"
     gene            complement(258..1205)
                     /gene="xerD"
                     /locus_tag="VIX62_03585"
     CDS             complement(258..1205)
                     /gene="xerD"
                     /locus_tag="VIX62_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY5859344.1"
                     /translation="MGEPITVEAERYLDHLTVERGLSEHTLMAYRRDLRRYLEFLAKR
                     DITEPGQVEEATIRSFVASLSAATYGEGEDERPYRASSVARTLSSVRSFHRFMVRDGV
                     ADRDPAGGVSQPKLPRSLPRPLTVEEVTRLLEAPDPATPVGIRDRAILELLYGAGLRI
                     SELVGLDVDDVDLDPGSVRVLGKGGKEREVPLGRYGREAVEAYVTRTRPEFASAKSRG
                     ALFLNQRGGRLTRQSCARLLAAHVATADIDRRVSLHTLRHSFATHLLEGGADVRVVQE
                     LLGHASVATTQIYTLVTREHLREVYYASHPRARRNARAR"
     gene            complement(1222..2346)
                     /gene="ald"
                     /locus_tag="VIX62_03590"
     CDS             complement(1222..2346)
                     /gene="ald"
                     /locus_tag="VIX62_03590"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY5859345.1"
                     /translation="MKVGIPKEVKDNEFRVATTPEGVRELVAGGHDVVVETAAGVGSA
                     IGDEDFVAAGASIAADADEVFAAADMIVKVKEPQPQEFARFREGQVLFTYLHLAADEG
                     LTRFLAERRVQSVAYETVQTPDGRLPLLAPMSEIAGRMAPQEGAAALERPHGGRGVLM
                     GGASGVAPARVVILGAGMAGANAAQIAAGMEAEVTVVDRNVERLRDIDRVWHGRIQTV
                     MSSSLAIERLVLSADLVVGAVLIPGAKAPHLVDAALVSRMQAGAVLVDISIDQGGCFE
                     TSHVTTHSDPTYIVDGVVHYCVGNMPGAVPRTSTYALTNVTLPYVSSIADLGLDAAII
                     HDPALALGVNVYGGAITNEPVAQAHGLAFAPLPDVAGGTA"
     gene            complement(2388..2924)
                     /locus_tag="VIX62_03595"
     CDS             complement(2388..2924)
                     /locus_tag="VIX62_03595"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5859346.1"
                     /translation="MPEPRSSRIDYEGSFVRVDVEEWPDLQPWEIVRLHDAAAVLPLL
                     PDDRVLLVKQFRPAVRQVLTEIPAGILDVDGEDALTAAARELEEETGYRHTTIEFLGG
                     YYSSAGSTDQYVHLFWARVEPEPVGAVEAGIELTTEPFARMVAAARSGKVRDAMTALA
                     LLMAAGRPPLPSPADARG"
     gene            complement(2928..>4522)
                     /locus_tag="VIX62_03600"
     CDS             complement(2928..>4522)
                     /locus_tag="VIX62_03600"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY5859347.1"
                     /translation="GLGKGITSASLGRLFKSRGLKVAVQKLDPYINVDPGTMNPFEHG
                     EVFVLDDGAETDLDLGHYERFIDTDLHRGSNHTTGAIYSAVIAKERRGDYLGKTVQVI
                     PHITDEIKERVLALAEAEAADLVLVEIGGTVGDIESLPFLEAIRQLRNEIGRDNCAFV
                     HVSLMPFIGPSGELKTKPTQHSVKELRALGIQPDAIVCRSDRPIGRNLKEKISLLCDV
                     PISGVISAPDAPSIYEVPLVLHHEGLDTELARHLGIEGEPDLSEWTGLVDRIHEVSEP
                     VSIAVVGKYVNMRDAYLSVVEALYHGGFHHGADVQIRWVASDDLGGQATDEALDGVDG
                     ILIPGGFGVRGVEGKVAAVRYARERHVPFLGICLGLQCAVIEFARTVVGLEGANSSEF
                     DPATPHAVIDLLPEQKDITDLGATMRLGAQPCYIEPDTLAAAAYGQEVVEERHRHRWE
                     VNPAYHEALTSAGMVISGSSQKGRLAEIIELRDHPFFIAGQFHPELRSRPTRPHPLFR
                     DFVGAAQVYRRQQAERQLSTTG"
BASE COUNT          699 a         1574 c         1567 g          682 t
ORIGIN      
        1 ggctcggccg tgcgcggttc cggcggccgc ccgcggcgcc acgccggcga tcggcgtcag
       61 caggccgagg ccgagctcct cgagcgccgg aacccgcaat ccgcccacca ccgccgcggt
      121 gtgggccagg gtgttcgagc cgtcgtcccc gtaggcgcga gcgtcgggcg cctcaccgac
      181 accccaggag tcgcagacga cgaccagcac cttcggtacg agcggttcag ccacggcggt
      241 cgtcctccac gatcggctca acgcgcccgg gcgttccggc gagcgcgtgg atgcgaagcg
      301 tagtagacct cccgcagatg ttcgcgggtg accagggtgt agatctgggt ggtggcgacg
      361 ctcgcgtgcc ccaggagttc ctggaccacc cggacgtcgg cgccgccttc cagcagatgg
      421 gtggcgaacg agtgtcgcag ggtgtgcagg ctcacgcggc gatcgatgtc ggcggtggcc
      481 acgtgggccg ccaacagccg ggcgcagctc tgacgcgtga ggcgcccccc tcgctggttg
      541 aggaagaggg ctccccggct cttggccgaa gcgaactccg ggcgcgtgcg cgtcacgtac
      601 gcctcgaccg cctcgcggcc gtatcggccg agcgggacct cgcgttcctt gccccctttg
      661 cccaggaccc ggacggatcc ggggtccagg tcgacatcgt cgacgtccag ccccacgagt
      721 tcgctgatcc gcaaaccggc gccgtacaac agctcgagga tcgcgcgatc acggatcccc
      781 accggcgtcg ccggatcggg ggcctcgagg agccgtgtga cttcctcgac ggtgagcggg
      841 cgcggcagcg agcgtggcaa cttcggctgc gacacaccgc ccgccgggtc acggtcggcg
      901 accccgtcgc gcaccatgaa ccgatggaac gagcgcaccg acgacagcgt acgggccacc
      961 gacgacgccc gatacggccg ctcgtcctcg ccctcgccgt acgtcgccgc gctgagggag
     1021 gccacgaacg agcggatcgt cgcctcctcg acctgtccgg gctccgtgat gtcgcgcttg
     1081 gccaggaact cgaggtaccg gcgaagatcc cgccgatacg ccatcagggt gtgctcggac
     1141 agaccccgct ccacggtgag gtgatccagg taccgctcgg cctcgaccgt gatcggctcc
     1201 cccaccgcga tgccccccca ctcaggccgt tccgcccgcc acgtcgggca acggggcgaa
     1261 ggccagaccg tgcgcctggg cgaccggttc gttcgtgatg gccccgccgt acacgttcac
     1321 gccgagcgcg agcgcagggt cgtggatgat cgccgcatcc aggccgaggt cggcgatcga
     1381 cgacacgtac ggaagcgtga cgttcgtgag cgcgtaggtc gaggtgcgcg gcacggcccc
     1441 gggcatgttg ccgacgcagt agtgcacgac gccgtcgacg atgtaggtgg ggtcagagtg
     1501 cgtggtgacg tgcgaggttt cgaaacaccc accctggtcg atcgagatgt cgacgaggac
     1561 cgcgcccgcc tgcatccgcg agaccaacgc ggcatcgacc agatgcgggg ccttcgcacc
     1621 ggggatgagc acggcgccga ccaccagatc ggcggacagc accagtcgct cgatcgcgag
     1681 gctcgagctc atcaccgtct ggatccgccc gtgccacacg cgatcgatgt cgcgcaggcg
     1741 ttcgacgttg cgatccacca cggtgacttc ggcctccatg cccgcggcga tctgggctgc
     1801 gttggctcct gccatccccg ctcccaggat cacgacgcgc gccggcgcca cgccggaggc
     1861 cccgcccatc aggacaccgc ggccgccgtg cggacgctcc aacgcggcgg cgccctcctg
     1921 cggcgccatc ctgccggcga tctccgacat cggggcgagc aatggcagcc ggccgtcggg
     1981 cgtctgcacg gtctcgtagg cgacgctctg cacgcggcgc tcggcgagga accgcgtgag
     2041 tccctcgtcg gccgcgaggt ggaggtaggt gaacagcacc tggccctccc ggaaccgcgc
     2101 gaactcctgg ggctgtggct ccttgacctt gacgatcatg tcggccgccg cgaagacctc
     2161 atcggcatcg gcggcgatcg atgcgccggc ggcgacgaag tcctcgtcac cgatcgcgga
     2221 cccgacgccc gcggcggtct ccaccacgac gtcgtgcccg ccggcgacca attcgcgcac
     2281 gccctcgggc gtcgtcgcca cgcggaactc gttgtccttg acttccttcg ggatcccgac
     2341 cttcacgccc tgcccttctc tcgagcgtca cgcctgcgca agcgtagtca accgcgtgcg
     2401 tccgcgggtg aaggaagtgg cgggcgaccg gcggccatca gcagggcgag cgccgtcatc
     2461 gcgtcacgga ccttgccgct gcgggccgcg gcgaccatgc gagcgaacgg ctcggtggtg
     2521 agctcgatgc cggcttcgac agcgcccacc ggttcgggct cgacacgggc ccagaacaga
     2581 tgcacgtact gatcggtcga gccggccgag gagtaatagc ccccgaggaa ctcgatcgtg
     2641 gtgtgccggt agcctgtctc ctcctccagt tcgcgcgctg ccgccgtgag ggcatcctca
     2701 ccgtcgacat cgaggatccc ggcggggatc tccgtgagca cctgccgcac ggcggggcgg
     2761 aactgcttca ccaacagcac ccgatcgtcg ggcagcagcg ggagcaccgc ggcggcgtca
     2821 tggagccgca cgatctccca cggctggagg tcgggccatt cctcgacgtc gacccggacg
     2881 aacgaaccct cgtagtcgat gcggctcgac cggggctccg gcacggctca gccggtggtc
     2941 gacagctggc gctccgcttg ctggcggcga tagacctggg cggcgcccac gaaatcgcgg
     3001 aacaacggat gcggccgggt gggacgcgat cgcaactcgg gatggaactg ccccgcgatg
     3061 aagaacggat ggtcgcgcag ctcgatgatc tcggccaggc gccccttctg cgacgaaccc
     3121 gagatgacca tgcccgccga ggtgagggcc tcgtggtagg cggggttgac ctcccagcga
     3181 tggcgatgcc gttcctcgac gacctcctgg ccataggcgg cggcggcgag cgtatccggc
     3241 tcgatgtaac agggctgcgc accgaggcgc atcgtggctc ccaggtcggt gatgtccttc
     3301 tgctcgggca gcaggtcgat gacggcgtgg ggcgtggccg gatcgaattc cgaggagttg
     3361 gcgccctcga gacccacgac ggtgcgggcg aactcgatca ccgcgcattg cagaccgagg
     3421 cagatcccca ggaacggcac gtgacgttcc cgcgcgtacc gcacggcggc caccttgccc
     3481 tcgacgccac ggaccccgaa gccgccgggg atcaggatgc catcgacccc gtcgagcgct
     3541 tcgtcggtgg cctggccgcc gaggtcgtcg gaggccaccc accggatctg gacgtcggcg
     3601 ccgtgatgga accccccgtg atacagcgcc tccaccacgc tgaggtaggc atcgcgcatg
     3661 ttgacgtact tgccgaccac cgcgatcgac accggttcgc tcacctcgtg gatgcgatcg
     3721 accaggcccg tccactcgga caggtcgggt tcgccttcga tcccgaggtg gcgggccagc
     3781 tcggtgtcga gtccctcgtg atgcagcacc agcggcacct cgtagatcga gggcgcgtcg
     3841 ggcgcgctga tcacaccgct gatcggcacg tcgcacagca gggagatctt ctccttgagg
     3901 ttgcggccga tcgggcggtc ggaacgacac acgatggcgt cgggctggat accgagcgcg
     3961 cgcagctcct tcaccgagtg ctgcgtgggt ttggtcttca gctcaccgga gggcccgatg
     4021 aacggcatca gggacacatg cacgaacgcg cagttgtcgc ggccgatctc gttccgtagc
     4081 tggcggatcg cctcgaggaa gggcagcgat tcgatgtcac cgacggtgcc gccgatctcg
     4141 acgagcacca ggtcggcggc ttcggcctcg gcgagcgcga gcacgcgctc cttgatctcg
     4201 tcggtgatgt gcgggatcac ctgcacggtc ttgccgaggt agtcaccccg gcgttccttc
     4261 gcgatcaccg cggagtagat ggcgccggtg gtgtggttgg acccacgatg caggtcggtg
     4321 tcgatgaacc gctcgtagtg accgaggtcg aggtcggttt cggcgccgtc atcgagcacg
     4381 aagacctcgc cgtgctcgaa ggggttcatc gtcccgggat cgacgttgat gtacgggtcc
     4441 agcttctgga cggcgacctt gagaccgcga gatttgaaga ggcgtccgag ggaggcggag
     4501 gtgatgccct tcccgagtcc gg
//
LOCUS       DATDBZ010000148         2821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1275537, whole genome shotgun sequence.
ACCESSION   DATDBZ010000148 DATDBZ010000000
VERSION     DATDBZ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275537"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VIX62_03605"
     CDS             complement(<1..613)
                     /locus_tag="VIX62_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5859348.1"
                     /translation="MSAVTIDQAEAVDRPPLRVKRKRWTAYILPVFTIAMIIYLAFPV
                     FVMILFSFNDLLGERQIAEFTCCTIRWWQDMFAIPNLNEALRTSILVAIPASIIATVL
                     GALIGLVLGRYQFSGRAVTNFVIFLAIAIPEIVLASSLLSMFVLGKTPIGFGTILFSH
                     IGFAIAFVAITVRARVQGLDRSLENAAQDLFATPFQAFMRVTMP"
     gene            complement(610..1566)
                     /locus_tag="VIX62_03610"
     CDS             complement(610..1566)
                     /locus_tag="VIX62_03610"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859349.1"
                     /translation="MPQDQAKDLALVTAAAVGQSGDGPPKRADQFLAKRQSRVAYGIL
                     APGGIYLMLAFIVPLILVVYTSLQSGGLLSGGYQFNWAFENYADAFSSSKTFIARALL
                     YSALATVLTILLAYPMAYWIAFYAGRWKSTLFFLILVPFFVSFVIRTVQWNFILADDG
                     VLFGFLKDLGVLPDDFRVLAGPVAVVAGITYNFLPFTALPLYVALDRIDKRLVEAAKD
                     LYATKFEAFRKVVLPLSFPGLFAALVLTFVPATGDYVNSSVLGSNRTTMVGQVIQTKF
                     LTEGDYPEAAALSVILMIGMLVIALAYAKVLGTEDSTLAAAA"
     gene            complement(1568..>2821)
                     /locus_tag="VIX62_03615"
     CDS             complement(1568..>2821)
                     /locus_tag="VIX62_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5859350.1"
                     /translation="SLMRGMTQRRVSRRDLIKYGGATVGTLSFASILAACGGDTAPGS
                     GGQTSGEPVDFSAPPGDTINFANWPLYIDSAKDPDTGERIHPSLVSFTDETGIAVNYN
                     DEIQSNASFFGELLPQLQAGQDTGRDIIVITNGREFTALTVNGWVTELDASMRPNFDA
                     NAATWAKDPDYDPGNKYSMAWQSGLTGIGYLTEKVSRPVATMDDLMNPDIVGTSSVGM
                     ITGDMPDLVMINLGIDPVTSGPAEWQEAADWVQMLKDSGTLRKFYDQGYIDDFTAGNL
                     AASMAWSGDVLYYKIWENKPYEWVVPEDGALLWIDNMMIPANSANPQGAYQLMDYVYQ
                     PEIAQLITEWVAYMSPVPAVQDLIAQHAAEESGEYAADLADMAENPLLWPDDALLAKT
                     PFGRNLTTDEEREEWDAIFVPITET"
BASE COUNT          515 a          906 c          930 g          470 t
ORIGIN      
        1 gcggcatcgt cacccgcatg aaggcctgga acggcgtcgc gaagagatcc tgcgccgcgt
       61 tctcgagcga tcggtcgagg ccctgcaccc gggcgcgtac tgtgatcgcc acgaacgcga
      121 tcgcgaaccc gatgtggctg aacaggatcg tgccgaaccc gatcggcgtc ttcccgagca
      181 cgaacatcga cagcagcgaa gaggcgagca cgatctcggg gatcgcgatc gcgaggaaga
      241 tcacgaagtt cgtcaccgcg cgcccactga actgataccg gccgagcacg agcccgatca
      301 gcgcaccgag caccgtggcg atgatcgacg cagggatcgc cacgaggatc gatgtcctca
      361 gcgcctcgtt caggttcggg atcgcgaaca tgtcctgcca ccaccggatc gtgcagcagg
      421 tgaattccgc gatctgccgc tcccccaaga gatcgttgaa cgagaacaag atcatgacga
      481 agaccgggaa cgccaggtag atgatcatgg cgatcgtgaa gaccggcagg atgtaggcgg
      541 tccagcgctt gcgtttgacg cgcagcggcg ggcggtcgac cgcctcggcc tggtcgatgg
      601 tgaccgcgct catgccgccg ccgccaacgt ggagtcttcg gtgcccagca ccttcgcgta
      661 cgcgagcgcg atcacgagca tgccgatcat caggatcacc gagagcgcgg ccgcctcggg
      721 atagtcgccc tccgtcagga acttcgtctg gatcacctgg ccgaccatcg tggtgcggtt
      781 ggagccgagc acgctggagt tcacgtagtc gcccgtggcc ggcacgaacg tcagcacgag
      841 ggccgcgaac agcccgggaa acgacagggg cagcacgacc ttgcggaacg cctcgaactt
      901 cgtggcgtac aggtccttcg ccgcctcgac gagccgcttg tcgatgcggt cgagggcgac
      961 gtagaggggc agcgcggtga acggcaagaa gttgtacgtg atgcccgcca cgacagcgac
     1021 cggcccggcg agcacccgga agtcgtcggg cagtaccccc aggtctttca ggaagccgaa
     1081 gagcacgccg tcgtcggcga ggatgaagtt ccactgcacg gtgcggatca cgaacgacac
     1141 gaagaatggc accaggatca ggaagaagag cgtcgacttc cacctcccgg cgtagaaggc
     1201 gatccagtac gccatcggat aagcgagcag gatcgtgagc acggtcgcga gggctgagta
     1261 cagcagggct cgagcgatga acgtcttcga ggacgagaac gcgtcggcgt agttctcgaa
     1321 ggcccagttg aattgatagc caccggagag caggcccccg ctctgcagcg aggtgtagac
     1381 cacgaggatc agcggcacga tgaaggcgag catcaggtag atgccgccgg gtgcgaggat
     1441 cccgtaggcg acccggctct gccgcttcgc gaggaattgg tcggcacgct tcggcggccc
     1501 gtcgccggac tgaccgacgg cggccgcggt gaccagcgcg agatccttgg cctgatcttg
     1561 tggcacccta cgtctccgtg atcgggacga agatcgcgtc ccactcctcg cgttcctcgt
     1621 cggtcgtgag gttgcgaccg aacggggtct tcgcaaggag agcgtcgtcg ggccacagga
     1681 gcgggttctc cgccatgtcc gccaggtccg ccgcatactc gccgctctcc tcggcggcgt
     1741 gctgagcgat gaggtcctgc acggcgggga ccggcgacat gtacgccacc cactcggtga
     1801 tcagctgcgc gatctccggc tggtagacgt aatccatcaa ctggtacgcg ccttgcggat
     1861 tggccgagtt cgccgggatc atcatgttgt cgatccacag gagcgccccg tcctcgggca
     1921 cgacccactc gtacggcttg ttctcccaga tcttgtagta gagaacgtcg cccgaccagg
     1981 ccatcgaggc cgcgaggttc ccggccgtga agtcgtcgat gtagccctgg tcgtagaact
     2041 tccgcagtgt gccggagtcc ttcagcatct ggacccagtc cgcggcctcc tgccactccg
     2101 ccgggcccga ggtgactggg tcgatgccga ggttgatcat caccagatcg ggcatgtctc
     2161 cggtgatcat gccgaccgat gacgtcccca cgatgtctgg gttcatcagg tcgtccatcg
     2221 tggcgaccgg ccggctcacc ttctcggtga ggtaaccgat gccggtgagg cccgactgcc
     2281 acgccatcga gtacttgttc cccgggtcgt aatcggggtc cttggcccac gtcgcagcgt
     2341 tcgcgtcgaa gttcggccgc atcgacgcat cgagctcggt cacccagccg ttcacggtca
     2401 acgccgtgaa ctctcgaccg ttcgtgatca cgatgatgtc acgtccggtg tcctgtcccg
     2461 cctggagctg cggcaggagc tcaccgaaga acgacgcgtt cgactggatc tcgtcgttgt
     2521 agttgaccgc gatgcccgtc tcgtcggtga acgacaccaa cgacgggtgg atgcgctcgc
     2581 cagtgtctgg atccttggcc gagtcgatgt acagcggcca gttcgcgaag ttgatcgtgt
     2641 cgcccggcgg cgccgagaag tcgacgggtt cgccgctcgt ctggccaccg ctgcccgggg
     2701 ccgtgtcgcc cccgcaggcc gcgaggatgg aggcgaaact cagggtgccg acggtggcgc
     2761 cgccgtactt gatcaggtcg cgccgcgaga cacgccgctg cgtcatcccc cgcatcaacg
     2821 a
//
LOCUS       DATDBZ010000149         8425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2507222, whole genome shotgun sequence.
ACCESSION   DATDBZ010000149 DATDBZ010000000
VERSION     DATDBZ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2507222"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VIX62_03620"
     CDS             complement(<1..468)
                     /locus_tag="VIX62_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY5859351.1"
                     /translation="MERLLVTGGARLEGAVVVSGAKNSALKLMAAALLAPGRTVLRNV
                     PRIQDCFTMGEVLEHLGAGVVWDGNTVTIDATTLTSVEAPYELVRRMRASTAVLGSLL
                     ARAGRARVAMPGGDEIGSRPIDLHIAGLRSMGAHVGSEHGFLVAETAGLRGAAI"
     gene            complement(507..923)
                     /gene="atpC"
                     /locus_tag="VIX62_03625"
     CDS             complement(507..923)
                     /gene="atpC"
                     /locus_tag="VIX62_03625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEY5859352.1"
                     /translation="MPLDVHVVTPEREIWSGEASTVVARGVAGEVGILSGHAPMMVGL
                     AIGPLRILRNDGPEITAVVDGGFMHVTSDGDATRVDVLAANAEMREEIDVEGARRRVS
                     ELEDRLRERDTADAEAELAGVKESLLKAQARINLAG"
     gene            complement(927..2369)
                     /gene="atpD"
                     /locus_tag="VIX62_03630"
     CDS             complement(927..2369)
                     /gene="atpD"
                     /locus_tag="VIX62_03630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY5859353.1"
                     /translation="MADTTPNGSNGHAAGRVVRVIGPVVDVEFPPGQLPEINTAVRID
                     VLLDEQTTTITCEVAQHIGDSQVRTIALKPTDGMVRGSPVENSGSPISVPVGEGVLGH
                     VYNVLGEPLDTTMDQIHPADYWPIHRDSPPFDELEPKSVMLETGIKVIDLLEPYVQGG
                     KIGMFGGAGVGKTVIIQEMIRRQAQQHGGVSVVAGVGERTREGNDLFLEMQESGVLAN
                     TALVFGQMDEPPGVRLRVALSALTMAEYFRDVERKDVLLFVDNIFRFTQAGSEVSTLL
                     GRMPSAVGYQPTLADEMGELQERITSTKGRSITSLQAIYVPADDITDPAPHTAFAHLD
                     ATTVLSRDIASLGIYPAVDPLDSTSRILDPRYVGEDHYAVARRVQEILQRNKDLQDII
                     AILGIDELSEEDKVIVSRARRIQRFLSQPFFVAEQFTGIEGKYVPVDETIASFKGICE
                     GEYDHLPEQAFFLVGGIEEAIEQAKTIEAA"
     gene            complement(2373..3263)
                     /locus_tag="VIX62_03635"
     CDS             complement(2373..3263)
                     /locus_tag="VIX62_03635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY5859354.1"
                     /translation="MGAQLRVVRRRIRSVQSTMKITRAMELIASSRIVKAQQRVEAAR
                     PYAIQLTQAMEDVAAQAGSVAHPLLEDREQPAKVGVLVVTSDRGLAGSYNSNVLKIAE
                     QQIESIRARGLEPVLYISGKKGVGYFRFRAVPIHREWQGHSEVPTYEVAQEVGHALIQ
                     DFAHQKIDELHCVYTDFRSAFTLRATHQRFLPIAPEEVTGTGGNPAAEYLYEPGPTEI
                     LDHLLPQYVITKVYAAMLESAASENAARRRAMKAATDNAEELIKIYTRQANRARQDEI
                     TTEISEIVGGAEALSAASGE"
     gene            complement(3490..5013)
                     /gene="atpA"
                     /locus_tag="VIX62_03640"
                     /pseudo
     CDS             complement(3490..5013)
                     /gene="atpA"
                     /locus_tag="VIX62_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            complement(5018..5575)
                     /gene="atpH"
                     /locus_tag="VIX62_03645"
     CDS             complement(5018..5575)
                     /gene="atpH"
                     /locus_tag="VIX62_03645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY5859355.1"
                     /translation="MPRDPDLISGYADAMFSVAGAEGVLTVVEDELYGFAKALEQNTS
                     LREALTDASLPAENKKAVVAELLGDRASPVTVNLLGFVIDAGRSREIPDVVQALAERA
                     AGERDHALAEVRSAVALSDAQRDRLAEALSQATGRTVDVKVVVDPAVVGGVVARVGDE
                     VFDGSVATRLDRAKQQLATGQQGSV"
     gene            complement(5577..6131)
                     /locus_tag="VIX62_03650"
     CDS             complement(5577..6131)
                     /locus_tag="VIX62_03650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY5859356.1"
                     /translation="MATMLALLAETAEETNDKADLYPHWEELVVGALAFAILFFFMWK
                     WVLPRLNTMLEERRAKIQGELEKAETTRKEADQLLADYRAQLADSKGEANRIIEEARA
                     AADQVRADIQAAANEEAQATVLRAQEEIRVERDRVFSELRAQVGEIAVELAGRVVGQS
                     LDESAHARLIDDYIDEVTRGNAQN"
     gene            complement(6150..6395)
                     /gene="atpE"
                     /locus_tag="VIX62_03655"
     CDS             complement(6150..6395)
                     /gene="atpE"
                     /locus_tag="VIX62_03655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY5859357.1"
                     /translation="MLALLAQAADASEGLATIGRGLVIGLAAIGPGIGLGVLIGKSVE
                     AMARQPEYAGQVRATMFIGIGLIEALALFGIAFSFII"
     gene            complement(6420..7214)
                     /gene="atpB"
                     /locus_tag="VIX62_03660"
     CDS             complement(6420..7214)
                     /gene="atpB"
                     /locus_tag="VIX62_03660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY5859358.1"
                     /translation="MNGLAGLIAAFEPPTTKDFVWGCWGPSLKIGSLSFCMNFINFLV
                     LFGMVLFIAFFYFGLRRTKVVPTKTQSLAEIGIDFVRENIAFPMLGADSDRFLPLLAT
                     FFFAIFFWNIFEVVPGINFSSNSRVAFPLVMAMISWVTYNGVGIAKHGVGGYLKHVCF
                     PPGAPWWLYPLLVPIEFFTQIIIRPITLAVRLWANFMAGHFLLAVFFLGTVAMLQGGF
                     TVIFAPFSFAIGVALVGFEIFVSGLQAFIFAILTASYIGGAMAEEH"
     gene            complement(7211..7636)
                     /locus_tag="VIX62_03665"
     CDS             complement(7211..7636)
                     /locus_tag="VIX62_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859359.1"
                     /translation="MVAAEPELELVRRVIPLSLPAIVVVALVAGLVGGTDAAISASLA
                     VVLVFANFVFFAVSVAYAARISLTLLYGVALGGFLVRMAILIVLLLLLEQLAWFSVAA
                     FVAAFVVGTVVLLSVEIKMIAGRMQADLWSFPEGQGARR"
     gene            complement(7630..7854)
                     /locus_tag="VIX62_03670"
     CDS             complement(7630..7854)
                     /locus_tag="VIX62_03670"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY5859360.1"
                     /translation="MAAPRQREAAWSGMGMGWSVTATMIAGILVWGGVGYVVDRLVGA
                     SHVFFALGTVLGACAATYIVYLRYGKGDPW"
     gene            complement(7982..>8425)
                     /locus_tag="VIX62_03675"
     CDS             complement(7982..>8425)
                     /locus_tag="VIX62_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5859361.1"
                     /translation="IGYPTILFSHIGFSIAFVAITVRARVQGLDRSLENAAQDLFATP
                     LVAFFKVTLPLIFPGVVAGFLLAFVLSLDDFVITNFGGGQTSTFPTWVYGATRIGVPP
                     QVNVWGTLLFGAGLAAASINLVSSIRKGRKQAAAIGGGVVKATSS"
BASE COUNT         1356 a         2859 c         2833 g         1377 t
ORIGIN      
        1 gatcgccgcc ccccggagtc cggcggtctc ggcgacgagg aacccgtgct cggatccgac
       61 gtgggctccc atgctgcgga ggcccgcgat gtgcaggtcg atgggacgcg acccgatctc
      121 atcgcccccg ggcatcgcca cgcgggctcg accggcccgc gccagcagcg agccgagcac
      181 ggcggtggag gcacgcatgc gccgcacgag ctcgtacggg gcctcgacgg acgtgagcgt
      241 cgtggcgtcg atcgtcacgg tgttgccgtc ccagacgacg ccggcgccca gatgctcgag
      301 gacctcgccc atggtgaagc agtcctggat ccggggcacg ttgcgcagca cggttcggcc
      361 gggcgcgagc agcgcggcgg ccatcagttt gagggcggag ttcttggcgc cggagacgac
      421 caccgccccc tccaggcgcg cccctcccgt gacgaggagg cgttccatga gggcatcagt
      481 gtaggggccg atggcccgtt ccggcctcag cctgccaggt tgatcctggc ctgggccttg
      541 aggagggatt ccttcacgcc ggcgagttcg gcctcggcat cggcggtgtc ccgctcgcgg
      601 agccggtcct cgagctcgct cacacgccgg cgggcgcctt cgacgtcgat ctcctcgcgc
      661 atctcggcat tcgccgcgag cacgtccacc cgtgtggcgt cgccgtccga ggtcacgtgc
      721 atgaacccgc cgtcgacgac cgccgtgatc tccggaccgt cgttgcgcag gatccgcagc
      781 ggcccgatcg ccaagccgac catcatcggg gcgtggccgg acaggatgcc gacctcaccc
      841 gctacgccac gcgccaccac ggtcgaggcc tcccccgacc agatctcgcg ctcgggcgtg
      901 acgacgtgga catccagggg catgcgctag gccgcctcga tcgtcttggc ctgctcgatc
      961 gcttcctcga tgccgcccac caggaagaac gcctgctccg gcaggtggtc atactcgccc
     1021 tcgcagatgc ccttgaacga ggcgatcgtc tcgtcgacgg gcacgtactt gccctcgatg
     1081 ccggtgaact gctcggccac gaagaacggc tgggacagga accgctggat acgtcgggcc
     1141 cgcgacacga tgaccttgtc ctcttcgctg agctcgtcga tgccgaggat cgcgatgatg
     1201 tcctgcaggt ccttgttccg ctggaggatc tcctgcaccc gccgggccac cgcgtagtgg
     1261 tcctcaccca cgtagcgagg gtcgaggatg cggctggtgg agtcgagcgg gtccacggcc
     1321 gggtagatgc ccagcgacgc gatgtcacgt gagagcaccg tggtggcgtc caggtgcgcg
     1381 aacgccgtgt gcggagccgg gtcggtgatg tcgtcggcag ggacgtagat cgcctgcagc
     1441 gaggtgatcg aacggccctt cgtcgaggtg atgcgctcct gcagttcgcc catctcgtcg
     1501 gcgagcgtgg gctggtagcc caccgccgac ggcatgcgcc ccagcagcgt cgagacctcc
     1561 gaaccggcct gggtgaatcg gaagatgttg tccacgaaca gcagcacgtc cttgcgctcc
     1621 acgtcgcgga agtactcggc catcgtcagc gcgctcagtg ccacgcgcag ccgaacgccg
     1681 ggcggctcgt ccatctggcc gaacaccagc gcggtgttgg ccaacacgcc ggattcctgc
     1741 atctccagga acaggtcgtt gccctcacgg gtgcgctccc ccacgccggc gacgacggac
     1801 acgccgccgt gctgctgggc ctgccggcgg atcatctcct ggatgatcac ggtcttgccc
     1861 acgcccgcgc cgccgaacat gccgatcttg ccgccctgca cgtagggctc gagcaggtcg
     1921 atcaccttga tgccggtttc cagcatcacg gacttaggct cgagctcgtc gaacggcggc
     1981 gagtcgcggt ggatgggcca gtagtcggcg gggtggatct gatccatcgt cgtgtcgagc
     2041 ggctcgccca gcacgttgta gacgtgcccc agcaccccct cgcccacggg cacgctgatc
     2101 ggcgagccgc tgttctccac gggggagccg cgcaccatgc cgtcggtggg cttcagcgcg
     2161 atcgtccgga cctgactgtc gccgatgtgc tgggccacct cgcacgtgat cgtcgtcgtc
     2221 tgctcgtcga gcagcacgtc gatccgcacg gcggtgttga tctcgggaag ctggccgggc
     2281 gggaactcga cgtcgaccac agggccgatg acccggacga cgcggccggc ggcgtgtccg
     2341 ttgcttccgt tcggggtggt gtcagccatt cgtcactctc ctgaagcggc ggacagggcc
     2401 tcggccccgc cgacgatctc actgatctcg gtggtgatct cgtcctggcg ggcgcggttg
     2461 gcctgccggg tgtagatctt gatgagctcc tccgcgttgt cggtcgccgc cttcatggcg
     2521 cggcggcgag ccgcgttctc ggaggccgcg gactcgagca tcgcggcgta gaccttggtg
     2581 atcacgtact gcggcagcag gtggtccagg atctcggtgg gacccggttc gtagaggtat
     2641 tcggcggcgg ggttgccgcc cgtgccggtc acctcttccg gcgcgatcgg caggaaccgc
     2701 tggtgggtgg cgcgaagtgt gaacgccgaa cggaagtcgg tgtacacgca atgcagctcg
     2761 tcgatctttt gatgggcgaa gtcctggatc agcgcgtggc cgacctcctg ggcgacctcg
     2821 taggtcggca cctcgctgtg accctgccac tcacggtgga tcggcacggc tcggaaccgg
     2881 aagtagccca cgcccttctt gccactgatg tacagcacgg gctccagccc tcgggcgcgg
     2941 atcgactcga tctgctgctc ggcgatcttc agcacgttgg agttgtagga gccggcgagc
     3001 ccgcgatcgg acgtgaccac gagcacgccc accttggcgg gctgctcccg gtcctccagg
     3061 agcgggtgcg cgaccgagcc ggcctgggcc gcgacgtctt ccatagcctg ggtgagctgg
     3121 atcgcgtagg gacgcgccgc ttccacgcgt tgctgcgcct tcacgatgcg cgaggacgcg
     3181 atcagctcca tcgcgcgggt gatcttcatc gtcgactgga ccgagcggat ccgtcgacgg
     3241 acgacgcgga gttgggctcc catcgcgtta cgtgggctcc tgcgcgccgg cctcgccgat
     3301 ggggcctgcc ccctcctcgg atccctccgt gcgctcctcg aggctgtctt cgacgcggcc
     3361 tgcagggccg tcatcgagat cgtcgtcggc cgaggacatg cgatcccagc ccacgtcggg
     3421 cttgacctcg tctggcggcg tggtcgagcc cagcgcggcg tcggaacccg gtccgcccga
     3481 cgtcgggacg aacgtctcct tgaacgcggc gatggcggcc ttgagcttgg cctcggtctc
     3541 gtcggagagc tcgccggtgg tgccgatcgt ctccaggatc tccgggctgc gggtctgcag
     3601 gtactcaccc agttcggtga cgaaccgctt cgcgtcctcg accggcaggt cgtcgagcgc
     3661 ggcgctcacc gtggcggcgt agacgtagac gacctgcatc tccatcggca gcggggcgtt
     3721 gcgaggctgc ttcagcacct cgatcacgcg agcgcctcgg gacagctgct gctgcgaggc
     3781 cttgtccagc tcggaaccga actgcgcgaa ggcttcgagc gcgcggtact gggccaggtc
     3841 caggcgcagg cgcccggcga cccgcttcat ggccttggtc tgcgcgttgc cgccgacgcg
     3901 gctcaccgac gtgccgacgt tgatcgcggg ccgcacaccc gagaagaaca gctcggactc
     3961 caggaagatc tgcccgtcgg tgatcgagat cacgttggtc gggatgtacg ccgagatgtc
     4021 gccgcccttg gtctcgatga tcggtagggc ggtcatcgaa cctccgccga gctcgtcgga
     4081 gagcttcgcg gcgcgctcca ggagccgcga gtgcaggtag aacacgtcac cggggtaggc
     4141 ctcgcggccc ggaggacggc gcagcaggag cgagagggtg cggtaggcgg tggcctgctt
     4201 cgacaggtcg tcgaagacga tcagcacggc ctcgccgttg tacatccagt acgcgcccag
     4261 cgccgcgccc gcgtacggcg cgatgtactg gaacggcacc gggtcggagg cggacgcgtt
     4321 gacgatggtc gtgtattcga gcgccccgtt ctcgcgcagc gtctctgtga cctcggcgac
     4381 ggtcgaggcc ttctggccga tggcgacgta gatgcacttg acctggttct cggtccccca
     4441 cgactcgcgc tgggcgatga tcgcgtcgac cgcgaccgcg gtcttgccgg tctggcggtc
     4501 gccgatgatc agctgacgct gaccccgccc gatggcggtc atcgcgtcga tcgccgtgat
     4561 gcccgtgtac aacggttctt tcaccggctg gcggctgacg acgttggcgg cctgcacctc
     4621 gaggttccgc gtctcggtgc tggcgatcgc tcctttgccg tcgatgggcc ggcccagcgc
     4681 gtcgacgacc cggccgagga acccgtcgcc caccggcatc gacaagatcg tcccggtctg
     4741 cttgacgggg tcgccttctt cgatgtggga ggactcgccc aggatcacgc aaccgatctc
     4801 atcttcgtcg aggttcaacg cgaggccgag cacgcccccg gggaactcga gcagttcgtt
     4861 cgccatggcg cggggcaggc cctgcacacg ggcgatcccg tcgccggtct cgacgacccg
     4921 gccgatctcc ccccgctcga cgctcggcga gtagctttcg acgttccttc ggagcgcctc
     4981 cgcgatgctc gcgggatcga tgttcagtgc cataccctca cacgctcccc tgctgtcctg
     5041 ttgcgagctg ctgcttggct cggtcgagcc gagtcgccac cgagccgtcg aagacctcat
     5101 cgccgacacg cgccacgacc ccaccgacca cggcgggatc gaccaccacc ttgacgtcaa
     5161 ccgtacgccc cgtcgcctgc gatagtgcct cggcgagccg gtcgcgctgt gcgtcgctga
     5221 gcgccacggc gctgcgaacc tctgcgagcg cgtgatcgcg ttcgccggcc gcccgctcgg
     5281 ccagcgcctg gacgacgtcc gggatctcgc gcgaacgtcc cgcgtcgatc acgaaaccca
     5341 gcaggttcac ggtgaccggg ctcgcgcggt cgcccaggag ctcggcgacc acggccttct
     5401 tgttctcggc gggcaacgag gcgtcggtga gcgcctcgcg cagcgacgtg ttctgttcca
     5461 aggccttggc gaagccgtag agctcgtcct cgaccacggt gagcacgccc tcggctccgg
     5521 cgaccgagaa catggcgtcg gcgtacccgc tgatcaggtc aggatcgcgt ggcatgtcag
     5581 ttctgcgcgt tcccgcgcgt gacctcgtcg atgtagtcgt cgatcaggcg ggcgtgcgca
     5641 ctctcgtcca aggactggcc gaccacccgc ccggcgagtt cgaccgcgat ctccccgacc
     5701 tgagccctga gttcggagaa gacccggtcg cgttcgacgc ggatctcctc ttgggcccga
     5761 agcacggtgg cctgggcttc ctcgttggcg gcggcctgga tgtcggcccg gacctggtcg
     5821 gcggccgcgc gggcttcttc gatgatccga ttggcctcgc ccttcgagtc ggcgagttgt
     5881 gcgcggtaat ccgcgaggag ttggtcggcc tccttccggg tggtttcggc cttctcgagc
     5941 tcgccctgga tcttggcgcg acgctcctcg agcatcgtgt tcagtcgcgg caggacccac
     6001 ttccacatga agaagaacag gatcgcgaac gccagggcgc ccacgacgag ttcctcccag
     6061 tgcgggtaga gatccgcctt gtcgttcgtt tcctcggccg tttcggcgag gagcgccagc
     6121 atggtggcca tcacgatgcg agccctcccc tagatgatga acgagaacgc gatgccgaac
     6181 agggccagcg cctcgatcaa gccgatgccg atgaacatgg ttgctcggac ctgccccgcg
     6241 tactcgggct gacgggccat cgcctcgacg gacttgccga tcaggacacc caagccgatg
     6301 ccggggccga tcgcggcgag accgatcacg agccctcggc cgatggtcgc cagaccttcg
     6361 cttgcatccg cggcctgcgc tagtagagcc aacatggtga acgcctcctt ggtcgttgat
     6421 cagtgctctt cggccatcgc gccgccgatg taggacgcgg tcaagatggc gaagatgaac
     6481 gcctgaagcc cggatacgaa gatctcgaag cccacgaggg cgacgccgat cgcgaatgag
     6541 aacggggcga agatgaccgt gaatccgccc tggagcatgg ccacggtgcc gaggaagaac
     6601 acggcgagca ggaagtggcc cgccatgaag ttggcccaga gtcggaccgc cagggtgatc
     6661 ggccggatga tgatctgggt gaagaactcg atcggtacca gcagcgggta gagccaccag
     6721 ggcgcgcccg gcgggaagca cacgtgcttc aggtagccac cgacgccgtg cttggcgatc
     6781 cccacgccgt tgtaggtgac ccacgagatc atggccatga ccaacgggaa cgcgacccgg
     6841 ctgttcgaag agaagttgat gcccggcacg acctcgaaga tgttccagaa gaagatcgcg
     6901 aagaagaagg tcgcgagcag cgggaggaat cggtcgctgt cggcaccgag catcgggaac
     6961 gcgatgttct cgcgcacgaa gtcgatcccg atctcggcca ggctctgcgt cttggtgggg
     7021 acgaccttcg ttcggcgcag gccgaagtag aagaaggcga tgaacagcac catcccgaac
     7081 aacaccagga agttgatgaa gttcatgcag aaggacaggc tgccgatctt gagcgaggga
     7141 ccccagcacc cccagacgaa gtctttcgtg gtgggcggct cgaacgccgc gatcagtccg
     7201 gcgagtccgt tcaacgccgc gccccttggc cctcagggaa gctccagaga tcggcctgca
     7261 tccgcccggc gatcatcttg atctcgaccg acagcagcac caccgtgccg acgacgaagg
     7321 cggccacgaa ggcggcgacc gagaaccacg ccagttgctc cagcagcagg agcaggacga
     7381 tcaggatcgc catccggacc aggaagccac cgagcgcgac cccgtagagc agcgtgagcg
     7441 agatgcgagc cgcgtaggcc accgacaccg cgaagaacac gaagttggcg aacacgagga
     7501 ccacggcaag gctcgcggag atcgcggcgt cggttccccc gaccaggccc gcgacgagcg
     7561 cgacgacgac gatggccggc aacgaaagcg gaatcaccct ccggacgagc tcgagttcgg
     7621 gctcagctgc taccacggat ctcccttgcc gtacctcaga tacacgatgt aggtggccgc
     7681 gcacgcgccc aagaccgtcc cgagggcgaa gaacacgtgc gacgctccga cgagtcggtc
     7741 gacgacgtac cccacaccgc cccacacgag gatgccggcg atcatggtcg cagtgacgct
     7801 ccaccccatc cccatgccag accaagcagc ttctcgttga cgtggggcag ccatgcgcgc
     7861 cggagtatag cggcggcccg acggccccgt caaaatcgcg agaacagcgt ttccgcaggt
     7921 ccgagggcat tttctcgtga ctttgtgaaa gttttcacga gccaggcggg cgcatctggg
     7981 atcaggaact cgttgccttc acgaccccgc caccgatggc cgcggcctgc ttgcggccct
     8041 tgcggatgct gctcaccagg ttgatcgagg cggccgccaa gccggcgccg aacaggagcg
     8101 tgccccacac gttcacctgc ggcggcacgc cgatgcgggt ggcgccgtac acccacgtgg
     8161 ggaacgtgct cgtctggccc cccccgaagt tcgtgatcac gaagtcgtcg agcgacagca
     8221 cgaaggccag gaggaatccc gccacgacgc cggggaagat cagcggcaag gtcaccttga
     8281 agaacgccac cagcggggtc gcgaacagat cctgggcagc gttctccagt gaccgatcca
     8341 agccttggac ccgcgcgcgc accgtgatcg ccacgaacgc gatcgagaac ccgatgtggg
     8401 agaacaggat cgtcgggtag ccgat
//
LOCUS       DATDBZ010000150         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1483026, whole genome shotgun sequence.
ACCESSION   DATDBZ010000150 DATDBZ010000000
VERSION     DATDBZ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483026"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VIX62_03680"
     CDS             complement(<1..138)
                     /locus_tag="VIX62_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY5859362.1"
                     /translation="MTDHTSRPSAADTEALETLRRLRGSIDNIDAALVYLLAERFRAT
                     KQ"
     gene            235..1392
                     /locus_tag="VIX62_03685"
     CDS             235..1392
                     /locus_tag="VIX62_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY5859363.1"
                     /translation="MTDGTPSPEPLAVSPAAGTDADATVPPPTPIPPHRPFWARLDSP
                     FTVGFLLTIGGLAAFVLGVAVSNIATVIIYIALAMFAALGLDPVIRWLGRHNVKRPWA
                     IAIVFLIFGVLGAGLLWLVVPTLIDQIGEFIASIPTTITNFESSDTYVWLEGIFGPGL
                     TTLVDEVQKFLLDPGNIAAISGGLLKVGVGIATALSGLLIIIVLTLYFVASLPGIKES
                     LMQFAPARNRPKVRSMTNQITESIGGYLMGMVILAFFNSVVAFLLHLFLGLPYPLLMG
                     VLAFSITIIPLVGPVLYWIVATVLALFTSPLAALIFGILYLIYIQIEAYVITPRVMSK
                     AISIPGSLVVIGALIGGTLLGLIGALVAVPVTASILLIIKQVYIPKQDAKV"
     gene            complement(1397..>2008)
                     /locus_tag="VIX62_03690"
     CDS             complement(1397..>2008)
                     /locus_tag="VIX62_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY5859364.1"
                     /translation="EDAAARVSRAHQSVFLPGGLLATTDMGLDLVRLWRTPDSGLRAL
                     QEVVLPRGSGPRHMVWHPSGHLYVVTELSREVFALAPDITGHWRIVAGTSLGAGMLDE
                     DTAAEIAASRDGDFLYAGVRGSNTIATVRVKGAGDVLAPVALVDAAVDWPRHHVVVRD
                     TLLVAGQRSDEVVSLTLDLRTGVPGRTRHRVEVPSPTCLLVVK"
BASE COUNT          306 a          709 c          638 g          355 t
ORIGIN      
        1 ctgcttggtc gcccggaacc gctctgcgag gagatagacg agcgcggcgt cgatgttgtc
       61 gatgctgcct cgaagccggc gcagggtctc gagcgcctcg gtgtctgcag cactgggccg
      121 agacgtgtga tcggtcatca ggttcctcct cgctccccga cgataccgcg cgaggagaaa
      181 ccgttgccga gcggcgccct gaccgccgcg gttcgcgacc cctagagtgt gtccatgacc
      241 gatggaactc cctcgcccga gccgctcgcg gtcagccctg cagcgggtac tgacgccgac
      301 gcgaccgtcc ctccgcctac accgatccca ccgcatcgac cgttctgggc acgcctggac
      361 agtccgttca ccgtgggatt cctgctgacc atcggcggcc tcgcagcgtt cgtgctcggc
      421 gtggccgtca gcaacatcgc caccgtgatc atctacatcg cactcgcgat gttcgccgcc
      481 ctggggctcg acccggtgat ccgatggctc ggccggcaca acgtcaagcg gccgtgggcc
      541 atcgccatcg tcttcctgat attcggcgtg ctcggcgcgg ggctcctctg gctcgttgtg
      601 cccaccctga tcgaccagat cggcgagttc atcgccagca tccccacgac gatcaccaac
      661 ttcgagagct ccgacaccta cgtctggctg gaggggatct tcggaccggg cctgaccacc
      721 ctggtggacg aggtgcagaa gttcctgctc gaccccggga acatcgcggc gatctcgggc
      781 ggtctgctga aggtcggcgt gggaatcgcg accgctctgt cgggcctgct catcatcatc
      841 gtgctgaccc tctacttcgt cgcctcgctc cccggcatca aggagtcgct gatgcagttc
      901 gcgccggcgc gcaaccggcc gaaggttcgc agcatgacca accagatcac cgagtcgatc
      961 ggcggctacc tcatgggaat ggtcatcctc gccttcttca actccgtggt ggcattcctc
     1021 ctgcaccttt tcctcggcct gccctacccc ctcctgatgg gcgtgctcgc cttctcgatc
     1081 acgatcatcc cgctcgtcgg cccggtgctg tactggatcg tcgccaccgt gctcgccctc
     1141 ttcacgagcc cgctcgcggc attgatcttc ggcatcctct acctgatcta catccagatc
     1201 gaggcgtacg tgatcactcc gcgggtcatg tcgaaggcga tctcgatccc cggatcactc
     1261 gtggtgatcg gcgccctgat cggcggcacg ctgctcggcc tgatcggcgc gctggtcgcg
     1321 gtacccgtca cggcgtcgat cctgctgatc atcaaacagg tctacatccc gaagcaggac
     1381 gcgaaggttt agcgccctac ttcaccacca gcaggcaggt cggcgacggc acctcgacgc
     1441 gatgacgcgt gcggccgggc acaccggtgc gcaggtcgag cgtgagtgag acgacctcgt
     1501 cggagcgctg cccggccacc agcagtgtgt cgcggaccac gacgtggtgc cgcggccagt
     1561 ccacggcggc atccacgagt gcgacgggcg cgagcacatc gccggcgccc ttcacgcgga
     1621 cggtggcgat ggtgttgctg ccgcgcacgc cggcatagag gaaatcgccg tcgcgggatg
     1681 ccgcgatctc ggccgccgtg tcttcgtcga gcattcccgc gccgagcgag gtccccgcga
     1741 cgatgcgcca gtgtccggtg atgtcggggg cgagggcgaa gacctcgcgg ctgagctcgg
     1801 tcacgacgta caggtggccg ctggggtgcc agaccatgtg tcgcgggcca ctgccgcgcg
     1861 gcagcaccac ctcctgaagg gcacgcagtc ccgaatcggg ggtgcgccag aggcggacga
     1921 gatccagacc catgtcggtc gtcgccagca ggccgcccgg gaggaacacc gactgatgtg
     1981 cgcgagagac gcgcgctgcc gcgtcctc
//
LOCUS       DATDBZ010000151         3464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1690570, whole genome shotgun sequence.
ACCESSION   DATDBZ010000151 DATDBZ010000000
VERSION     DATDBZ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690570"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VIX62_03695"
     CDS             <1..645
                     /locus_tag="VIX62_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY5859365.1"
                     /translation="AVIDLKEYSEGRFLLFVTRKGIVKKTKLPEYDSPRTGLIAVKLR
                     GDDELIDVRFTDGSDDVFLVSRKGQAIRFKESLVRPMGRDAGGVIGMRLADDDRVLAV
                     GLASDGEEMISVTEQGYGKRSKLADYPIKGRGGKGVIGHQLTGKTGLLAGAYIGSKDI
                     DMFVISSSGQVVRVGAGDIRRVGRSSQGVRTMRVEDDATVVALAPVIIQMDDEE"
     gene            complement(681..1442)
                     /locus_tag="VIX62_03700"
     CDS             complement(681..1442)
                     /locus_tag="VIX62_03700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY5859366.1"
                     /translation="MAIAVTGSLVIAGLVVAANAESSVDVALRQKHDLQKRIEELQET
                     RRLRRVGLHQRIRFTQARLRNAPGAANVGDRARSRSYRQLQIERIEDLRAEERELVRT
                     TRARIRDLRSKRTGLAEWIESLPLQRCPVDGPVAISDGFGDIRDMPGTPRHIHQGNDI
                     MAPTGAPIIAPFDGEAVAGSSDLGGTSVKVYGAGGYVYNAHLSAYGKLGAVQAGDVIG
                     YVGSTGNATAPHNHFEWHPGDGAAVDPYLFLTAVC"
     gene            1606..1863
                     /locus_tag="VIX62_03705"
     CDS             1606..1863
                     /locus_tag="VIX62_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859367.1"
                     /translation="MDGGPAAIGLGTALFLVGLFGVRSGRALGVWSTVLAASGAGIAI
                     GCLLVQDQVDTASWIIAPVAGAILAVVHAHVLFAPGGPFRT"
     gene            2171..2560
                     /locus_tag="VIX62_03710"
     CDS             2171..2560
                     /locus_tag="VIX62_03710"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEY5859368.1"
                     /translation="MRSAPRRTRVVVRKVGPLSVLKFSLLFYLCLMLIVLFAMVIVYG
                     VLAAAGAIDSLETVLGYVFGTGTTSTGGAEPIEIDGGSLFMWGLFGGLTFVAVWSVIN
                     VFIAMLYNLISDIIGGVEVTLAERPRH"
     gene            2639..2713
                     /locus_tag="VIX62_03715"
     tRNA            2639..2713
                     /locus_tag="VIX62_03715"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2674..2676,aa:Ile,seq:gat)
     gene            2737..2928
                     /locus_tag="VIX62_03720"
     CDS             2737..2928
                     /locus_tag="VIX62_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859369.1"
                     /translation="MTDEATVTVEAVEEKKKGGFLKKLMKIAVIGAIVAAVVAFVKRR
                     RGDDLDDVEWQELPPPAGG"
     gene            3061..>3464
                     /locus_tag="VIX62_03725"
     CDS             3061..>3464
                     /locus_tag="VIX62_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5859370.1"
                     /translation="MDGPAWALANDAASAAGVRLRPLTSLDDADAILRVMMATWSEAI
                     PRETVRALAGSGNVPWGAHDGNELVGYVLGWAAISPGDGLHEHSHMLAALPDRRHAGV
                     GYALKLAQRAACLDRGIHLVRWTFDPLVARNA"
BASE COUNT          501 a         1161 c         1224 g          578 t
ORIGIN      
        1 gccgtgatcg acctgaagga gtactcggag gggcgcttcc tgctgttcgt cacccgcaag
       61 ggcatcgtga agaagacgaa gctgcccgag tacgactcgc cccgaaccgg gttgatcgcc
      121 gtcaagcttc gtggtgacga cgagctgatc gacgtgcggt tcaccgatgg cagcgacgac
      181 gtgttcctcg tctcgcgcaa gggccaagcg atccggttca aggaatcgtt ggtacggccg
      241 atgggccgcg acgccggcgg cgtgatcggc atgcgcctgg ccgatgacga tcgcgtgctc
      301 gcggtcggcc tcgcctccga cggcgaggag atgatcagcg tgacggagca gggctacggc
      361 aagcggtcca agctcgccga ctacccgatc aagggccgtg gaggcaaggg cgtgatcggg
      421 caccagctca ccggcaagac gggactgctc gccggtgcct acatcggctc gaaggacatc
      481 gatatgttcg tgatctcgtc gagcggccag gtcgtgcgcg tcggcgcggg cgacatccgg
      541 cgcgtcggcc gctcgagcca gggggtacgg acgatgcggg tggaggacga tgccaccgtc
      601 gtggcgctcg cccccgtgat catccagatg gacgacgagg agtagggcgg ggacgccccc
      661 gccctaccga tcgtctcgtc tcagcagacc gccgtgagga acaggtaggg gtccaccgcc
      721 gcgccgtcgc ccggatgcca ctcgaagtgg ttgtgcggcg ccgtcgcgtt gccggtgctg
      781 ccgacgtagc cgatcacgtc gccggcctgc acggcgccga gcttgccgta ggccgacaga
      841 tgggcgttgt agacgtatcc gcccgcgccg tagaccttga ccgaggtgcc tccgagatcg
      901 ctggagccgg cgacggcctc gccgtcgaac ggcgcgatga tcggcgcgcc ggtcggagcc
      961 atgatgtcgt tgccctggtg gatgtggcgg ggcgtgcccg gcatgtcacg gatgtccccg
     1021 aagccgtcgg agatcgccac ggggccgtcg acagggcatc gctgcagtgg aagtgattcg
     1081 atccactccg caagcccggt gcgcttcgac ctgaggtcgc ggatgcgcgc gcgggtcgtg
     1141 cggaccagtt cgcgctcctc ggcgcgcagg tcctcgatcc gctcgatctg cagctggcgg
     1201 tagctacggg aacgcgcccg gtcgccgacg ttggcggccc ccggggcgtt gcggagacgc
     1261 gcctgggtga accggatgcg ctggtgcagc cccacacggc gcaggcgtct ggtctcctgc
     1321 aactcctcga tgcgcttctg gagatcgtgc ttctggcgca gggcgacgtc cacggatgat
     1381 tccgcgttgg ccgccacgac cagcccggcg atcaccaagc ttcctgtcac tgcgatcgcc
     1441 atcaggcgtg ccgtacggtt ccgcatcaga tcgttctccc tctcccctcg atgtcctact
     1501 gcggtatcgg cagggaggac ggcgggcctt gagcgatccg ggtgagaact acgtagtcat
     1561 ctctgcctga tgtcggcgga ggctacgtac gggatgatgc ggtccatgga cggcgggccc
     1621 gcggcgatcg ggttggggac ggcgctcttc ctcgtcggtc tgttcggcgt gcgctccggg
     1681 cgggcgctgg gggtgtggtc caccgtgctc gccgcctccg gcgccggcat cgcgatcggc
     1741 tgcctgctcg tgcaggatca ggtcgatacc gcctcgtgga tcatcgcgcc ggtcgcaggc
     1801 gcgatcctgg ccgtggtaca cgcccacgtg ctgttcgcgc ccggcggccc gttccgaacg
     1861 tgaggctccg ggtacttgcg ttggccaggg cgtaccctta ggacttcgtc ccgaggagat
     1921 ggtgcacacg gccgagcaac ctgagaccgc cgaaccgcgt cagccgcagc gcggccccga
     1981 cacgggtgtg tcggtgccac agccgcgcgt ggtcgcgacc gcgatcgacg ccccggcgcc
     2041 ggcagcgtcc cccaccccgt caccagcgcc gaccaccccg acgccgtcct ccggacccga
     2101 gggatcgttc gccgccggcc cgcagacgcc tcgcggggcc cgcgcggctc agccctcacg
     2161 cccgccacgc atgcgctcgg cgccgcgccg cacccgggtg gtggtgcgca aggtcgggcc
     2221 gctgtcggtc ctgaagttct cgctgctgtt ctacctgtgc ctgatgctga tcgtgctgtt
     2281 cgcgatggtg atcgtttacg gggtcctcgc agccgcgggg gcgatcgact cgctcgaaac
     2341 ggtgctcggc tacgtcttcg gtacgggcac gacctcgacc gggggcgcgg aaccgatcga
     2401 gatcgacggt ggctcgctct tcatgtgggg gctcttcggc gggctgacct tcgtcgcggt
     2461 ctggtcggtg atcaacgtct tcatcgcgat gctctacaac ctgatcagcg acatcatcgg
     2521 cggggtcgag gtcaccctcg ccgagcgacc acgtcattga cggccgtcga acgttcgttt
     2581 gacacgtgtt tctggccgtg gctacgctgc cacgagggcg accggccccg atgagttggg
     2641 gcctgtagct cagcccggtt agagcgcttc cctgataagg aagaggtcga tggttcaagt
     2701 ccatccaggc ccactcccgg aaggagggct cctcccatga cagatgaagc caccgtgacg
     2761 gtcgaggccg tcgaggagaa gaagaagggc ggattcttga agaagctcat gaagatcgcc
     2821 gtgatcggcg cgatcgtcgc ggcggtcgtg gcattcgtga aacgtcgccg cggcgacgat
     2881 ctcgatgacg tcgagtggca ggaactgccg ccgcccgccg gcggctgatc gagccaccgc
     2941 cggttgtgcc ggcggttcgt tccacggaag gcccgccatg cggcgggcct tccgcctttc
     3001 gtcgaccgtc acggcctgga gggcgactgc ccgaaggggc ccgtcgatag actccggcgc
     3061 gtggacggac ccgcgtgggc actggcgaac gacgcggcgt cggcggccgg tgtgcgcctg
     3121 cgccccctca cctccctcga cgacgcggac gcgatcctgc gcgtcatgat ggccacgtgg
     3181 agtgaggcga tccccaggga gacggtcagg gcgctcgcgg gatccggcaa cgtgccgtgg
     3241 ggtgcgcacg acggcaacga gttggtcgga tacgtgctcg gctgggccgc catctcgcct
     3301 ggcgacggac tccacgaaca ctcgcacatg ctcgccgccc tccccgatcg gcggcatgcc
     3361 ggcgtcggtt acgcgctcaa gctcgcacaa cgcgcggcgt gcctcgatcg cggcatccat
     3421 ctcgtgcgct ggacgttcga cccgttggtc gcccgcaacg cgca
//
LOCUS       DATDBZ010000152         3347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2099532, whole genome shotgun sequence.
ACCESSION   DATDBZ010000152 DATDBZ010000000
VERSION     DATDBZ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2099532"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VIX62_03730"
     CDS             complement(<1..830)
                     /locus_tag="VIX62_03730"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY5859371.1"
                     /translation="MRGTSAPPAGLAAGLATTTYPRRTFVRTAGITAALASTGIAADL
                     FRALPASAGGTVSFVPTDPDLHLLRRATYGITPNLQKDIRRLGRNRWLEQQLDPTSIN
                     DAFVEDYIHDRIPNLNMGLQEAWDTLGGSWDLMVDLGKAAILRAGWSKRQLYEVMVDF
                     WSNHLNITNPHESCWWSRHDYDRKVIRKHTFGKFSNMLRDSALHPAMMMYLNNAESTK
                     DNPNENYGREILELHSVGVDGGYDEDDMRQSTLVLTGYGIDWDTGLYEYHDWAHYTGP
                     V"
     gene            complement(919..2118)
                     /locus_tag="VIX62_03735"
     CDS             complement(919..2118)
                     /locus_tag="VIX62_03735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY5859372.1"
                     /translation="MSGTLGGALERRLLSIVGGTIRVGQLTVSLPDGTEREFRGRFDG
                     PEAHVDLHDWRLLRRLTSTGAIGLADGYVAGEYDSPDLSAFLELAAWHLEPAYRYEVP
                     GWLHAVGRTAWKLLGASAKPRGPLQDIVQHYDLGNDFYEAWLDETMTYSSAVFADERM
                     TLAEAQAEKYRRLAAATGIGPGDHVLEIGSGWGGFAAMLADEIGCHVTTITVSKQQHD
                     HVEKLIADRGLADRVQTQLRDFRDVRGDYDHIVSVEMMESIPASLWSPFFGQLRSLTK
                     PGGTIGLQLIEVAEHHWSISDTNLDFVRRYIFPGGQVPSEPVLRDLASAFGLRWRANR
                     EYGQDYARTLRAWLAGFDAAWPSIRGMSAGFDDRFRRMWRYYLAYCGAGFAVGRVDVS
                     QIVLERP"
     gene            complement(2115..3023)
                     /locus_tag="VIX62_03740"
     CDS             complement(2115..3023)
                     /locus_tag="VIX62_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEY5859373.1"
                     /translation="MGATADPGTERGGGFRLPLGGASGLYRGRVMHQRSFPRSHRLSY
                     GVWYLLADLDELPRLDRTTPGFTYNRPGPVSFWDRDHGSRDGAPLRPWIEGRLAEAGV
                     DLEGGPIRVLSFPRVFGYGFNPICVWWCHGPAGDLRAILYEVSNTFGEWHDYLVPVRP
                     EDVIANGRGASVRTAFAKELYVSPFIDMEATYDFTTREPDDRVSVVVRESTRGGRVLI
                     ATLSARRLALTGRTLLTTLLRFPLVTLKVIGGIHWEAWKLWRKGAPFRRRGLPPAAPV
                     SIVGPRAPEVTEGATAQVLSRRRVLT"
     gene            complement(3031..>3347)
                     /locus_tag="VIX62_03745"
     CDS             complement(3031..>3347)
                     /locus_tag="VIX62_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859374.1"
                     /translation="NLRTDRPVLVTLNPPHEPRDATAETVYHHPQYDGDAVEAQRRIP
                     ALQGVDRTWFAGSYTGFGFHEDALRSGLNVAAALGSPAPWWRDEIEHSTHDERQPLMI
                     GG"
BASE COUNT          515 a         1123 c         1180 g          529 t
ORIGIN      
        1 accgggccgg tgtagtgcgc ccagtcgtgg tactcgtaca accccgtgtc ccagtcgatg
       61 ccgtagcccg tcagcaccaa cgtcgactgg cgcatgtcgt cttcgtcgta gccgccgtcg
      121 acgcccaccg agtgcagctc caggatctcc cgaccatagt tctcgttcgg gttgtccttg
      181 gtcgactcgg cgttgttgag gtacatcatc atggccgggt gcagggcgga gtcgcggagc
      241 atgttcgaga acttgccgaa cgtgtgcttg cggatcacct tgcggtcgta gtcgtggcgc
      301 gaccaccagc aggactcgtg cgggttcgtg atgttgaggt ggttggacca gaagtcgacc
      361 atcacctcgt acagctggcg cttgctccat cccgcgcgaa ggatcgcggc cttgcccagg
      421 tccaccatca ggtcccacga cccgcccagg gtgtcccagg cttcctgcag ccccatgttc
      481 aggttgggga tgcggtcgtg gatgtaatcc tcgacgaagg cgtcgttgat cgaggtcgga
      541 tcgagctgtt gttccagcca ccggttgcgt cccaggcgcc tgatgtcctt ctgcaggttc
      601 ggtgtgatgc cgtaggtcgc ccggcgcagc aggtgcagat ccggatcggt cggtacgaac
      661 gaaacggtgc cgccggccga ggcgggtagc gcccggaaca ggtcggcagc gatgccggtg
      721 ctcgcgagcg cggccgtgat cccggcggtg cgcacgaacg tacggcgtgg ataggtcgtg
      781 gtcgccagac ccgcagccag accagcaggt ggtgcggatg tccccctcat ggagggttct
      841 atcggtcgca ggggtggtcc aacttgaccc cccaggggtg actactgccg ttcggccgtc
      901 cgggtgcgtc cgttcgggct aggggcgttc gagcacgatc tggctgacgt cgacccgacc
      961 caccgcgaac cctgcgccgc aatacgcgag gtagtagcgc cacatcctcc ggaaccggtc
     1021 gtcgaagccg gcgctcatcc ctcggatcga gggccacgcg gcgtcgaaac cggcgagcca
     1081 ggcgcggagg gtgcgcgcgt agtcctggcc gtactcccgg ttcgcccgcc agcgcagacc
     1141 gaacgccgag gcgaggtcgc gcagcaccgg ttcggacggc acctggccgc cggggaagat
     1201 gtagcggcga acgaagtcga ggttcgtgtc cgagatcgac cagtgatgct cggccacctc
     1261 gatcagctgc aggccgatcg tgcctcccgg tttcgtgagc gaccggagct gtccgaagaa
     1321 gggtgaccac aacgatgcgg ggatcgactc catcatctcc accgagacga tgtggtcgta
     1381 gtcgccgcgc acgtcccgga agtcccggag ctgggtctgc acgcgatcgg cgagcccgcg
     1441 atcggcgatc agcttctcga cgtggtcatg ctgctgcttg gagacggtga tcgtggtgac
     1501 gtggcagccg atctcgtcgg ccagcatcgc ggcgaaccca ccccagcccg acccgatctc
     1561 gagcacgtgg tccccgggac cgatccccgt cgcggcggcg agccgccggt acttctcggc
     1621 ctgcgcctcg gccagggtca tgcgctcgtc ggcgaagacg gccgagctgt aggtcatcgt
     1681 ctcgtcgagc caggcctcgt agaagtcgtt gcccaggtcg tagtgctgca cgatgtcctg
     1741 cagcgggcca cgcggtttcg ccgatgcacc gagcagcttc cacgcggttc gtccgacggc
     1801 gtggagccac cccggcacct cgtagcggta ggcgggttcc aggtgccagg ccgcgagttc
     1861 caggaaggcg gacaggtcgg gggagtcgta ctcgcccgcg acgtagccgt cggccagtcc
     1921 gatcgccccg gtggacgtga gccgtcgaag caggcgccag tcgtggagat cgacgtgtgc
     1981 ctcgggcccg tcgaagcggc cgcggaactc ccgctccgtg ccgtcgggca ggctcacggt
     2041 cagctggccg acccggatcg tgccgccgac gatggagagc aaccggcgct cgagggcgcc
     2101 gcccagcgtg ccgctcatgt cagaacccta cgcctggaga ggacttgcgc ggtagcccct
     2161 tcggtcacct ccggagcacg cggaccgacg atcgacaccg gggcggcagg cggcaggccg
     2221 cgacgtcgga acggagcgcc cttgcgccag agcttccacg cctcccaatg gatcccgccg
     2281 atcaccttca gcgtgaccag cgggaaccgc aggagcgtcg tgaggagcgt gcgccccgtg
     2341 agggcaagcc ggcgcgcgct cagcgtggcg atcaggaccc ggccgccccg cgtggactcg
     2401 cgcaccacca ccgagacccg gtcgtcgggt tcgcgcgtgg tgaagtcata ggtggcctcc
     2461 atgtcgatga acggcgagac gtagagctcc ttcgcgaacg ccgtgcgcac cgaagcgccc
     2521 cgcccgttcg cgatcacgtc ctcgggccgg accggcacca ggtagtcgtg ccactcgccg
     2581 aacgtgttcg agacctcgta gaggatcgcg cgcaggtcgc cggccggacc gtggcaccac
     2641 cagacgcaga tcgggttgaa gccgtagccg aacacgcgtg ggaacgacag cacgcggatc
     2701 ggtccaccct cgaggtcgac gccggcctcc gccaggcgcc cttcgatcca cggacgcaac
     2761 ggagcgccgt cgcgcgatcc gtggtcgcgg tcccagaagc tgaccgggcc gggacggttg
     2821 taggtgaagc cgggggtcgt gcggtcgagg cggggcagct cgtcgaggtc ggcgagcagg
     2881 taccagacgc cgtaggacag gcgatggctt cgggggaagg atcgctgatg catcacccgg
     2941 ccgcgataga gcccggaggc gccgccgagg ggcaaccgga agccgccgcc tcgctcggtc
     3001 ccgggatcgg cggtcgcacc catcgccggc tcagcctccg atcatgaggg gctgtcgctc
     3061 gtcatgggtg ctgtgctcga tctcgtctcg ccaccacggt gcgggtgagc ccagcgcggc
     3121 cgccacgttc agccccgaac ggagcgcgtc ctcgtggaac ccgaaccccg tgtaactgcc
     3181 ggcgaaccac gttcggtcca cgccctggag cgcggggatc cgccgttgcg cctcgaccgc
     3241 gtcgccgtcg tactgcgggt gatggtagac ggtctcggcc gtggcgtcgc gcggctcgtg
     3301 gggcgggttc agggtcacca gcaccgggcg gtcggtccga aggttct
//
LOCUS       DATDBZ010000153         5866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1053801, whole genome shotgun sequence.
ACCESSION   DATDBZ010000153 DATDBZ010000000
VERSION     DATDBZ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053801"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VIX62_03750"
     CDS             <1..563
                     /locus_tag="VIX62_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY5859375.1"
                     /translation="CATDRLEARARGGRSSFLRPSVTFRDEILAANTRFSSGLPTLDR
                     AARPTRAIAVLACMDARLRPEEALGLELGEAHVIRNAGGRASDDAIRSLALSSHVLGT
                     RAFAVVHHTDCGLLGTTNDELHGRIAATTGADVRAIDFLPFDDATQALVEDVERIRRS
                     SFIDDEVEVIGLRYDVRTGAIEIVVP"
     gene            complement(560..1015)
                     /locus_tag="VIX62_03755"
     CDS             complement(560..1015)
                     /locus_tag="VIX62_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5859376.1"
                     /translation="MTTPRRTSPAREVDAVADRLHSAAIHLLRRLRVEDEALGISAPR
                     LSVLSVLVFAGPMRIGELAHAEQVEPPTMTRLVDGLQRDGYVAREPDPDDARAVRVRA
                     TDAGTAALTEGRRRRVAAFSDLLRTLPHRELTALGRGVDALERALERER"
     gene            1074..1730
                     /locus_tag="VIX62_03760"
     CDS             1074..1730
                     /locus_tag="VIX62_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY5859377.1"
                     /translation="MTSTSADLFEAIEQGDTSRVRTILAAEPTLAETRDREGVSALMR
                     TRYRMDKDLIQAVHEHVDELDIFEASTFGDLDRLTELLAYDPASVVERTGDGFTALHF
                     AAFFGGPEVTSFLVTRGSQVDAHGTGWMTGTPLHSAASSDHVDVARVLLDAGASPDER
                     QSGGWTPLHAAAHNGSAAMVTLLLEAGADPTLVNDDGRTALDLATEQGEPATIALLGG
                     "
     gene            complement(1741..2820)
                     /locus_tag="VIX62_03765"
     CDS             complement(1741..2820)
                     /locus_tag="VIX62_03765"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEY5859378.1"
                     /translation="MTPPTIDILRVEGSHREVGRQLGEMGRDRIQDELATAFDDLPAA
                     RSRDQQLALATGYRDLTRDRLPWLIDELDGCAEGAGVDPLAFFACSMEEIWYEPRQRV
                     TQGRCSDVVAGPTATANGHLLVGHTNDLGPAMEDQIVAIEKRVTGDPTIFQLGGIPWL
                     SVGWNSAGLSLTGNELSPNDEQVGISRSHQVLEMMRARTLDEMVAMALRPDRASSYNN
                     VLTSADGGVANVEGSATDAEVTGLDAAGHLAHTNHYVCDRMLPFEGDPGYAKRSAVRF
                     ERARTLLAEQPPASITETTLRTVLSDHEGAPDSICRHPEPGDGEGSRTVFWCVADVTR
                     GRITFGRGNPCDSVAQDYAFSPYAA"
     gene            complement(2848..3786)
                     /locus_tag="VIX62_03770"
     CDS             complement(2848..3786)
                     /locus_tag="VIX62_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY5859379.1"
                     /translation="MRRSTRITSLAVVLGASLALGVAAASAQTLDPTGESAEPYIVLT
                     GHLTVEAGTTVEDAIIFDGDATIDGNVSGNAVALNGDIVVNGDVRGDVIALNGRVTVA
                     ATGSVTGNVMSRLEPALDGEVGGDVQRNGLRTDGLLVFGRVAFWLAASISSLLFGLLW
                     FGFFPRGGEAVANAARTAVGGSIGFGFMLFLGIPILGVIMLVTIVGGLFGVALLTGMV
                     LLYSVAYTAGALALGRLLLKEPRRLVVAFLLGWVILRVLALVPVLGGTLFAAATVWGF
                     GAIVVAAWRTGHDRPRPAVMAPAVTAGETPPMPPLP"
     gene            complement(3807..5177)
                     /locus_tag="VIX62_03775"
     CDS             complement(3807..5177)
                     /locus_tag="VIX62_03775"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEY5859380.1"
                     /translation="MTETAVAPGIDRRKLAELKEREDAAFIGSHAGSIERWNQAKAVM
                     PNGVPMSWLRTSYDHPPLFVGEGKGGHFRDVDGNDYADFNIADMSMFGGYAPEPVVEA
                     VALRMAAGSQFLLPSEDSIWVAQELQRRFGLPKWQFTLSATHANTEAIRVARTITGRD
                     RVLLFDGKYHGHFDETLVELEDGHLVPGEVGLPRDVTSKTTIVQFNDVEALRAALETR
                     EIAIVLTEPVLTNEIGLQLPVEDFHAELRAITRETGTLLAYDETHTQVVGPGGLTRMW
                     GLEPDIVSAGKSLAGGIPMGTYGMTEAVADVLERPSGRDDEKPSVATGGTLFGNPLQM
                     ASARATMGEVLTDQAYAHTHVLGTQLADGIEATIDKAGLPWTAHRFWPRSGVTFAERM
                     PRNALEARETFDVPLRRLMRVYFANRGIWDAIVGAGPTCAVPATVDDVDAYIGAFSDL
                     VAELTA"
     gene            complement(5219..>5866)
                     /locus_tag="VIX62_03780"
     CDS             complement(5219..>5866)
                     /locus_tag="VIX62_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5859381.1"
                     /translation="APTGDAPAVGGGVVVVASDSVEGGVSAFPAVCTEGCEPLWTADT
                     DGPATGVTIEGDTAYVVAHGSLLAFPLSCQGACHPTWTGTFVPGGAFAEGSPAPPGVG
                     SGQVAIAGDDGTLWLFPATCPNPACGPLRSFDLGAEGLGRPVIEEGFVFVAAEDGRLF
                     AIGTGCDPSNVTTCDEAATTSLGSPTRAVPASGAGVVYAGDDAGTVHAFDLRASG"
BASE COUNT          890 a         2045 c         2059 g          872 t
ORIGIN      
        1 gatgcgccac ggaccgtctg gaagcacggg ctcgtggtgg ccggtcgtcg ttcctgagac
       61 ccagcgtgac cttccgtgac gagatcctgg cggcgaacac ccgattctcc tcgggcctgc
      121 ccacgctcga tcgtgcggcc cgccccacgc gagcgatcgc ggtgctggcc tgtatggatg
      181 cccggttgcg ccccgaggag gccctgggcc tggagctcgg cgaagcccac gtgatccgca
      241 acgccggcgg ccgcgccagc gacgatgcga tccgatccct cgcgctgtcc tcgcacgtac
      301 tgggcacccg cgcgttcgcg gtggtccatc acaccgattg cggcctgctc gggaccacga
      361 acgacgaact ccacggccgc atcgccgcga ccaccggcgc cgacgtgcgg gcgatcgact
      421 tcctgccctt cgacgacgcg acgcaggcct tggtcgagga cgtggaacgg atccgcagat
      481 cctcgttcat cgatgacgag gtcgaggtga tcgggttgcg ctacgacgtt cggaccggcg
      541 cgatcgagat cgtcgtcccc tagcgttctc gttcgagggc ccgctccagg gcatccacgc
      601 cgcgaccgag cgcggtgagc tcccggtggg ggagagttct tagcagatcg gagaacgcgg
      661 ccaccctgcg ccggcgccct tcggtgagcg ccgccgttcc ggcgtccgtg gcacgcaccc
      721 ggaccgctcg agcatcgtcg gggtcgggtt cacgcgccac gtagccgtcg cgctgcaggc
      781 cgtcgaccag ccgggtcatc gtggggggtt cgacctgttc tgcgtgtgcc agctcgccga
      841 tccgcatggg accggcgaac accaggaccg agagcacgga gaggcgcggg gcagagatgc
      901 ccagcgcctc atcctccacg cgcaggcgac gcaacaggtg gatcgcagcc gaatggagcc
      961 ggtcggcgac cgcgtccacc tctcgcgctg gggaagtccg ccgaggcgtg gtcatcttgc
     1021 ttcgtccgtc atatttagtt aggctacgca aatacaacca cgggaggcaa accgtgacaa
     1081 gcacatcggc cgacctcttc gaggcgatcg agcagggcga cacctcgcgg gtgcggacga
     1141 tcctggccgc cgagccgacg ctggcggaga cgcgggatcg cgaaggggtc tcggcgttga
     1201 tgcgaacgcg ctaccggatg gacaaggacc tcatccaggc ggtgcacgag cacgtggacg
     1261 agctcgacat cttcgaggcg tcgaccttcg gcgatctgga ccggctcacc gagctgctgg
     1321 cctacgaccc ggcctcggtg gtggagcgca cgggtgacgg attcaccgcg ttgcacttcg
     1381 ccgccttctt cggcggcccg gaggtcacct cgttcctggt gacgcgcggc tcgcaggtgg
     1441 acgcgcacgg caccggctgg atgaccggga cgccgctgca cagcgcggcg agcagcgatc
     1501 acgtcgacgt ggcccgggtg ctgctggacg cgggcgcttc accggacgaa cgtcagtcgg
     1561 gcggctggac accgttgcat gccgccgccc acaacgggag cgcggcgatg gtgacgctcc
     1621 tgctggaggc cggcgccgac ccaactttgg tgaacgacga cggccgaacg gcgttggatc
     1681 tggccaccga gcagggagag cccgcgacga tcgccctcct cggcggctga gcccggccga
     1741 tcaggccgcg tacggcgaga acgcgtagtc ctgcgccacg gagtcgcagg ggttgccgcg
     1801 gccgaacgtg atccgaccgc gggtgacgtc ggcgacgcac cagaacacgg ttcttgatcc
     1861 ctcaccgtcg ccgggctccg gatggcgaca gatggagtcg ggcgcgccct cgtggtcgga
     1921 caagacggtg cgcagggtgg tctcggtgat cgatgcgggc ggctgttcgg cgagcagggt
     1981 ccgggcacgt tcgaaccgca ccgccgaacg cttcgcgtag ccggggtcgc cttcgaacgg
     2041 cagcatgcga tcgcacacgt agtggttcgt atgggcgagg tgtccggcag cgtccagccc
     2101 ggtgacctcg gcgtcggtgg cgctgccctc gacgttggcc acgccgccgt cggcgctcgt
     2161 caacacgttg ttgtagctgg aggcgcggtc gggtcgcagc gccatcgcca ccatctcgtc
     2221 cagggttcgg gcacgcatca tctcgagcac ctggtgggac cgagagatcc cgacctgctc
     2281 gtcgttgggg gagagctcgt tgccggtgag cgagagcccg gcgctgttcc agccgacgga
     2341 gagccagggg atacccccca gttggaagat cgtggggtcg ccggtcaccc gcttctcgat
     2401 cgcgacgatc tggtcctcca tcgccgggcc gaggtcgttg gtgtgtccca ccaggaggtg
     2461 gccgttcgcc gtggcggtgg gcccggccac gacgtcgctg catcggccct gggtcacgcg
     2521 ctgacgcggt tcgtaccaga tctcttccat cgaacatgcg aagaacgcga gtggatcgac
     2581 gccggcaccc tcggcgcacc cgtcgagctc atcgatcaac caggggagcc ggtcgcgcgt
     2641 gaggtcgcgg tagccggtgg cgagtgcgag ctgctgatcg cgggagcgag cggcgggcag
     2701 gtcgtcgaac gcggtggcca gctcgtcctg gatgcggtcg cggcccatct cgcccagctg
     2761 gcgtcccact tcgcggtgcg atccctcgac gcgcaggatg tcgatcgtcg gtggtgtcat
     2821 ggtccgatgg tagcggtgcc gatgtcccta gggcagcggc ggcatcggcg gggtctcgcc
     2881 cgccgtcacg gccggagcca tcaccgcggg acggggcctg tcgtgaccgg tccgccacgc
     2941 cgccaccacg atcgcgccga atccccacac cgtggccgcg gcgaacagcg tgccgcccag
     3001 gacgggcacg agcgccagca cgcgcaggat gacccacccc agcaggaacg ccacgacgag
     3061 cctccgtggc tccttcagga gcagccggcc cagcgcgagg gcgccggcgg tgtaggcgac
     3121 cgagtagagc agcaccatgc ccgtgagcag ggccaccccg aacaatccgc ccacgatcgt
     3181 gacgagcatg atcaccccga ggatcgggat gcccaggaac agcatgaacc cgaacccgat
     3241 cgagccgccc accgcggtcc tggcggcgtt ggcgacggcc tcgcctcccc gcgggaagaa
     3301 cccgaaccac aacaggccga acagcaggga cgagatcgaa gccgcgagcc agaacgccac
     3361 ccggccgaag accagcaggc cgtcggtgcg aagcccgttg cgctgcacat cgccgccgac
     3421 ctcgccgtcc agggcgggtt ccagccggga catgacgttg ccggtcacgg atccggtcgc
     3481 cgccacggtg acccggccgt tgagcgcgat gacgtcgccg cgcacatcac cgttgacgac
     3541 gatgtcgccg ttgagcgcca ccgcgttccc gctgacgttc ccatcgatcg tggcgtcacc
     3601 gtcgaagatg atcgcgtctt cgacggtggt gccggcctca acggtgagat gcccggtgag
     3661 cacgatgtag ggctcggccg actcgccggt cgggtcgagg gtctgggccg atgctgcggc
     3721 gacgccgagg gcgagggacg ctcccagcac caccgcgagg ctcgtgatcc gtgtgcttcg
     3781 tcgcatgcgg gcacctccaa ggttggtcaa gcggtgagtt cagcgaccag gtcggagaac
     3841 gcgccgatgt aggcgtcgac gtcatccacc gtcgcgggca cggcgcacgt cggcccggcg
     3901 ccgacgatcg cgtcccagat gccccggttc gcgaagtaga cacgcatcag acgccggagc
     3961 ggtacgtcga aggtctcgcg agcctccagc gcgttgcgcg gcatccgctc ggcgaacgtg
     4021 accccgctcc gtggccagaa ccggtgggcg gtccacggca ggccggcctt gtcgatcgtg
     4081 gcctcgatgc cgtcggcgag ctgggtgccc agcacgtggg tgtgggcgta cgcctggtcg
     4141 gtgagcacct cgcccatcgt ggcgcgggcc gacgccatct gcagcgggtt gccgaacagg
     4201 gtgccgccgg tggccacgga cggcttctcg tcatctcgcc cactggggcg ttcgaggacg
     4261 tcggccacgg cctcggtcat gccgtacgtg cccatcggga tgccgccggc cagcgacttg
     4321 cccgccgaca cgatgtcggg ctcgagtccc cacatgcggg tcaggccacc ggggccgacg
     4381 acctgcgtgt gcgtctcgtc gtaggcgagc agggtgccgg tctcgcgggt gatcgcgcgg
     4441 agctcggcgt ggaagtcctc gaccggcagc tgcagaccga tctcgttcgt gaggacgggc
     4501 tcggtgagca cgatcgcgat ctcgcgggtc tccaaggccg cccgcagcgc ctcgacgtcg
     4561 ttgaactgca cgatcgtggt cttcgacgtg acgtcgcgcg gcaggcccac ctcgccgggg
     4621 accagatgtc cgtcctccag ctccaccagg gtctcgtcga aatgcccgtg gtacttgccg
     4681 tcgaacagca gcacccggtc ccggccggtg atggttcgag ccacacggat cgcctcggtg
     4741 ttggcgtggg tcgccgacag ggtgaactgc cacttcggca ggccgaaccg gcgctgcagc
     4801 tcctgagcca cccagatcga gtcctcgctc ggcagcagga actggctccc ggccgccatg
     4861 cgcaacgcca ccgcctccac cacgggttcg ggcgcgtagc cgccgaacat ggacatgtcg
     4921 gcgatgttga agtcggcgta atcgttgccg tccacgtcgc ggaaatgccc gcccttgccc
     4981 tcgccgacga acagcggagg gtggtcgtag gaggtgcgga gccacgacat cgggacaccg
     5041 ttcggcatca ccgccttcgc ctggttccag cgttcgatcg atcctgcgtg gctgccgatg
     5101 aaggcggcgt cctcgcgttc cttgagctcc gcgagcttgc ggcggtcgat gcccggtgcg
     5161 acggcggtct cggtcatgct ctaggtctat cagtcgttcc gtcgcctggc caacgcacct
     5221 agccggaggc gcgcagatcg aaggcgtgca ccgtcccggc atcgtcaccc gcatagacca
     5281 caccggcgcc cgatgccggc accgcgcggg tcgggcttcc gagcgaggtc gtggccgctt
     5341 cgtcgcacgt ggtcacgttc gaggggtcgc agccggtgcc gatcgcgaac aggcggccgt
     5401 cctcggccgc gacgaacacg aagccctctt cgatcaccgg cctgcccagg ccttcggctc
     5461 cgagatcgaa cgaccgcagt ggcccgcacg cggggttcgg acacgtggcg gggaacaacc
     5521 agagcgtccc gtcgtcgccc gcgatcgcca cctgtccgga accgacgccc ggcggggccg
     5581 gcgacccttc cgcgaacgca ccgcccggca cgaacgtccc ggtccaggtg gggtgacacg
     5641 cgccctggca cgagagcggg aacgccagca gcgatccgtg cgctaccacg taggcggtgt
     5701 cgccctcgat cgtgaccccg gttgccggtc cgtcggtgtc ggcggtccag agcggttcgc
     5761 acccttccgt gcacaccgcg gggaacgccg agacgccgcc ttcgaccgag tcggatgcca
     5821 ccaccacgac cccgccgccc accgcggggg catcgcccgt cggggc
//
LOCUS       DATDBZ010000154         2333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_872000, whole genome shotgun sequence.
ACCESSION   DATDBZ010000154 DATDBZ010000000
VERSION     DATDBZ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872000"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /gene="ftsH"
                     /locus_tag="VIX62_03785"
     CDS             complement(<1..1520)
                     /gene="ftsH"
                     /locus_tag="VIX62_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY5859382.1"
                     /translation="MLVTLLFLLALSLFTSEGGGNEMSFSDFQTAVADGRVQDATIHD
                     ADQTVTGDLEDGTGYTTSYPTEYADDLAGILQKAGVPTEVDPQKPNALLSTFITVLLP
                     VLLIGGILLFVMNRSQGGGGRVMQFGRSKHKTVNKDQPKTTFADVAGVDEAIEELQEV
                     KEYLQAPAKFQAMGAKIPRGVLLFGPPGTGKTLLARAVAGEAGVPFFTISGSDFVEMF
                     VGVGAARVRDLFEQAKATAPAIVFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMD
                     GFDQRSTVILMAATNRPDILDPALLRPGRFDRQVVIDRPDLEGRKAILRVHARGKPFD
                     QTVDLAVLARRTPGFTGADLANVINEGALLAARRNKRAISMKEIEEAVDRVMAGPERK
                     SRVMDEEERRLIAYHEGGHAMVAHVLPNTDEVHKITVIPRGRALGYTLTLPEQDKFLM
                     TREQMRDELAMLMGGRVAEEIVAGDVTTGAANDIERATKVARQMVTEYGMSDTIGPR"
     gene            complement(1682..2233)
                     /gene="hpt"
                     /locus_tag="VIX62_03790"
     CDS             complement(1682..2233)
                     /gene="hpt"
                     /locus_tag="VIX62_03790"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY5859383.1"
                     /translation="MEAYEPDIEKVLISSEEIEAKLVEMGEAITRDYDGRSLLLVGVL
                     KGAFVVMADLARHIRLPLEFDFMAVSSYGAATKSSGVVRILKDLDHDLEGRDVLLVED
                     IVDSGLTLKYLLKNLAARKPASLEVAAMLRKTGIQKVPLDLRYIGFDIPPEFVVGYGL
                     DYAERFRNLPYVATLKPEAYGGA"
BASE COUNT          359 a          778 c          806 g          390 t
ORIGIN      
        1 cgcgggccga tcgtgtcgct catgccgtac tcggtgacca tctggcgcgc caccttggtg
       61 gcgcgttcga tgtcgttggc cgctccggtg gtgacatcgc cggcaacgat ctcctccgcg
      121 acccgcccgc ccatgagcat cgcgagctcg tcgcgcatct gctcgcgcgt catgaggaac
      181 ttgtcctgct cgggaagggt gagcgtgtag ccgagcgcgc gcccgcgcgg gatcaccgtg
      241 atcttgtgga cctcgtcggt gttcggcagg acgtgcgcca ccatggcgtg gccaccttcg
      301 tggtaggcaa tcaggcggcg ctcctcctcg tccatcacgc ggctcttgcg ctcggggccg
      361 gccatgacgc ggtcgacggc ttcctcgatc tccttcatgg agatggctcg cttgttgcgg
      421 cgtgccgcca acagggcacc ttcgttgatg acgttggcga gatcggcgcc ggtgaacccg
      481 ggggtgcgcc gcgccagcac cgcgagatcg acggtctggt cgaacggctt gccgcgcgca
      541 tggacgcgca ggatcgcctt gcggccctcg aggtcaggac ggtcgatcac gacctgacgg
      601 tcgaaccgcc cgggccgcaa gagcgcgggg tcgaggatgt cggggcggtt cgtggccgcc
      661 atcaggatca ccgtggagcg ctgatcgaac ccgtccatct cgaccaacaa ctggttcagg
      721 gtctgctcgc gctcgtcgtg accgccaccc atcccggcgc cccgctggcg ccccaccgca
      781 tcgatctcgt cgatgaacac gatggcaggg gccgtcgcct tcgcctgctc gaacaggtcg
      841 cgcacccgcg cggcgccgac ccccacgaac atctcgacga agtcggagcc gctgatcgtg
      901 aagaacggca cgccggcctc cccggccacg gcgcgagcga gcaacgtctt gccggtaccg
      961 ggaggaccga agagcaggac cccccgcggg atcttggcgc ccatggcctg gaacttcgcc
     1021 ggcgcctgca ggtactcctt gacctcctgc agttcctcga tcgcctcgtc gaccccggcg
     1081 acgtcggcga aggtggtctt gggctggtcc ttgttgaccg tcttgtgctt gctccgaccg
     1141 aactgcatga cccggccgcc gcccccctgc gagcggttca tgacgaacag caggatgccg
     1201 ccgatcagca gcacggggag cagcacggtg atgaaggtgg acagcagggc attcggcttc
     1261 tgcggatcga cctcggtggg cacgcccgcc ttctgcagga tgccggcgag gtcgtcggcg
     1321 tactccgtcg ggtagctggt ggtgtagccg gtgccgtcct cgagatcacc ggtgaccgtc
     1381 tggtcggcgt cgtggatcgt ggcgtcctgg acgcgcccgt cggccaccgc ggtctggaag
     1441 tccgagaacg acatctcgtt cccgccgccc tcgctggtga acagcgacaa cgccagcagg
     1501 aacaggagcg tgaccagcac gatccagagc gtcggggtgc gcgcgaaacg cttcagcggg
     1561 ggctcgggtt gctgactcag ggctgctcct tgccgtgcgg gaagacggtg cgaggctcac
     1621 gcgccggacg cacgggagcc cgggcacgat ggtagcaccg gcccccgacc ggaggggagg
     1681 ctcaagcgcc gccgtacgcc tcgggcttga gcgtggccac gtagggcagg ttgcggaagc
     1741 gttcggcgta gtcgagaccg tagcccacga cgaactccgg cgggatgtcg aagccgatgt
     1801 accgcaggtc gagcggcacc ttctggatcc cggtcttgcg cagcatggcg gccacctcga
     1861 gcgaggcggg cttgcgcgcg gcgaggttct tcaacaggta cttgagcgtc aggccggaat
     1921 ccacgatgtc ctcgaccagc agcacgtcgc ggccttccag atcgtggtcg aggtccttga
     1981 ggatccgcac cacgcccgag gacttggtgg cggcgccgta ggacgagacc gccatgaagt
     2041 cgaactccag cggcagccgg atgtgacgag ccaggtcggc catgaccacg aaggcgccct
     2101 tgagcacacc gaccagcagc agcgatcgtc cgtcgtagtc ccgggtgatg gcctcgccca
     2161 tctcgacgag cttggcctcg atctcttcgg acgagatcag gaccttctcg atgtcgggtt
     2221 cgtaggcctc cactggaagc gtgagtgtag ctgcgggcgt ccggtgggtg acggtcacgg
     2281 gtcggtccct tccgtgcgat cggtccgaac cggggaagcg atgcgaacat act
//
LOCUS       DATDBZ010000155         3993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_259844, whole genome shotgun sequence.
ACCESSION   DATDBZ010000155 DATDBZ010000000
VERSION     DATDBZ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259844"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="hisH"
                     /locus_tag="VIX62_03795"
     CDS             complement(<1..440)
                     /gene="hisH"
                     /locus_tag="VIX62_03795"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY5859384.1"
                     /translation="MTTIAVLDYGSGNLHSVSRALARVGGTPLITGDPAEVGNADALV
                     IPGVGHFGACMRAICHHGLDGSIRDFVASGKPVFGVCVGMQVLFDGSDEDAEPGLSVI
                     SGASRLLPDDVKVPHMGWNTVEWRTDHPYVAGVDDATRFYFVHS"
     gene            complement(437..1021)
                     /gene="hisB"
                     /locus_tag="VIX62_03800"
     CDS             complement(437..1021)
                     /gene="hisB"
                     /locus_tag="VIX62_03800"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY5859385.1"
                     /translation="MSRTANVTRTTKETDIRVELNLDGSANSTADTGIPFFDHMVQQL
                     GKHAGWDLDVTCKGDLAVDGHHTVEDVGIAIGQALSEALGDKAGVRRFASIVVPLDEA
                     AVEVALDLSGRNFVVFDVPMPAETIGEFDTGLVEDFVRAFAQSADVTIHIHLRYGRSP
                     HHVCEATFKGMAKALGDAVSLTGHAGVPSTKGTL"
     gene            complement(1018..2076)
                     /gene="hisC"
                     /locus_tag="VIX62_03805"
     CDS             complement(1018..2076)
                     /gene="hisC"
                     /locus_tag="VIX62_03805"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY5859386.1"
                     /translation="MSPAAQPRPGVRDVEPYLSPQLEVVARLNTNECPHPLPQDISDA
                     LAGAIRDLPLNRYPDGQMTRLREEVAAHHGHPFEGTWTANGSNEILTELLLAYGGPGQ
                     RAAIFEPTYLLHSRLSWLTHTEVIAHRLDDPFILNEHAVEQALADEPDVVFVCSPNNP
                     TGNAQPAQAVAELAQASPALIVVDEAYIEFGGESALKLVADNPNVAVVRTFSKAFALA
                     GARLGYVLTSPEVVSDLQRVRLPYHMSSLTQAAGIAAIAHTNEAMTVLEGIRAQRDRI
                     DRELRAIEGITVYPSDANFVLFVPPGDAKQVWQGLLDRSVLVRDVTAVVPNALRVTAG
                     AEHEVDLFLTSLQEVLQA"
     gene            complement(2073..3365)
                     /gene="hisD"
                     /locus_tag="VIX62_03810"
     CDS             complement(2073..3365)
                     /gene="hisD"
                     /locus_tag="VIX62_03810"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY5859387.1"
                     /translation="MLQFLDLRERGERLEATRLETDPTVADTVTGILQRVFVEGDDVL
                     IELAQRFDGCDISETGVLVTEAEFKDAEADTPPDLRAALDALIERLKDLAARQLPRGW
                     VDERDGVRFGEIVRPLESVGCYVPGGRAVYPSSVCMTVVPAVVAGVEEIVLCTPPQAD
                     GSIPDPVLYAAMRAGATYVAKTGGAQAVGAMAYGTESIPRVDRIVGPGNAYVTEAKRQ
                     VAGAVGIDGLAGPSELTIVAAGDIDIDMAALDLIAQAEHDPEARTFFVSPDPELIERV
                     GEALEEALEDAGRREIVDAALRYTKAVLVRDLDQATDVVNDLAAEHLQILLPDPEVFL
                     EGVRNAGAVFLGRWTAVPFGDYGVASNHVLPTAGTARFSSGLRAADYVTVSSFVQMDA
                     GAATRFAPGTSAIAEAEGLVGHARAMNARADATEVVEP"
     gene            complement(3366..3515)
                     /locus_tag="VIX62_03815"
     CDS             complement(3366..3515)
                     /locus_tag="VIX62_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859388.1"
                     /translation="MDRETPLLIAVVMLSLAALTVGVSGWVLLVRDRRLSRVAAVHPE
                     GPEES"
     gene            complement(3566..3925)
                     /locus_tag="VIX62_03820"
     CDS             complement(3566..3925)
                     /locus_tag="VIX62_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5859389.1"
                     /translation="MADIDVRFYQLVIDCADPRSLAAFWMRFTGFEIGSAHDDWVSIR
                     SHDNGTRIAFQRVPESKAGKNRLHVDFAAKDEAATASAIEAIGATRRWASDDPEDPFI
                     VLADPEGNEFCIVRDDG"
BASE COUNT          619 a         1350 c         1413 g          611 t
ORIGIN      
        1 aacgagtgca cgaagtagaa gcgagtggcg tcgtcgacgc cggcgacgta gggatgatcg
       61 gtgcgccact ccacggtgtt ccagcccatg tgcggcacct tcacgtcgtc gggcagcaag
      121 cgtgaggcgc ccgagatgac ggacaagccg ggttcggcgt cctcatcgct gccgtcgaac
      181 agcacctgca tgcccacgca cacgccgaac accggcttgc cggaagcgac gaagtcgcgg
      241 atcgagccgt cgagcccgtg gtggcagatg gcgcgcatgc aggcgccgaa gtggccgacg
      301 ccggggatca ccagggcgtc ggcgttcccc acttcggccg ggtcgcccgt gatcaacggg
      361 gtgccgccga cacgcgccag cgcgcgcgag accgaatgga ggttgccgga gccgtagtcg
      421 agcaccgcga tcgtcgtcac agggttcctt tggtcgaggg gacgccggcg tgtccggtga
      481 ggctcaccgc atcgcccagc gccttcgcca tacccttgaa cgtggcttcg cagacgtggt
      541 ggggggagcg cccgtagcga aggtggatgt ggatcgtgac gtcggcgctc tgcgcgaacg
      601 cccgcacgaa gtcctccacc agcccggtgt cgaactcccc gatcgtctcg gccggcatcg
      661 gcacgtcgaa caccacgaag ttgcggccgg acagatcgag cgccacctcg accgcggcct
      721 cgtcgagcgg caccacgatc gaggcgaacc gccgaacgcc cgccttgtcg cccaacgcct
      781 cgctcaacgc ctggccgatc gcgatgccga cgtcctccac cgtgtggtgg ccgtcgacgg
      841 ccaggtcgcc cttgcacgtc acgtccaggt cccagcccgc gtgcttgccc agttgctgca
      901 ccatgtggtc gaagaacggg atgccggtgt cggcggtcga gttcgccgag ccgtccaggt
      961 tcagttcgac ccggatatcg gtctccttcg tggtgcgcgt gacgttggcg gtgcggctca
     1021 tgcttgcagg acctcctgga ggctggtgag gaacaggtcg acctcgtgct cggcgccggc
     1081 ggtgacgcgc aaggcgttgg gcaccactgc cgtgacgtcg cgaaccagga cgctgcgatc
     1141 cagcaacccc tgccacacct gcttcgcgtc gccggggggc acgaacagca cgaagttggc
     1201 gtccgatggg tacaccgtga tgccctcgat cgcgcgcagt tcgcggtcga tccgatcgcg
     1261 ctgcgcacgg atcccctcca gcaccgtcat ggcctcgttc gtgtgcgcga tcgccgcgat
     1321 gccggcggcc tgggtcagtg aggacatgtg atacgggagc cgaaccctct ggaggtccga
     1381 caccacttca ggcgacgtga gcacgtagcc gagccgggcg cccgcgagcg cgaacgcctt
     1441 ggagaacgtg cgcaccacgg cgacgttcgg gttgtcggcg accagcttca gcgcactctc
     1501 gccgccgaac tcgatgtagg cctcgtcgac cacgatcagc gccggcgacg cctgcgcgag
     1561 ttcggcgacc gcctgcgcgg gctgggcgtt gccggtcggg ttgttgggcg agcagacgaa
     1621 caccacatcg ggttcgtcgg ccagggcctg ttcgaccgcg tgctcgttga ggatgaacgg
     1681 gtcgtcgagc cggtgcgcga tcacctcggt gtgcgtgagc cacgacaggc gcgagtgcag
     1741 caggtacgtg ggttcgaaga tcgcggcgcg ctgccccggg ccgccgtagg cgagcagcaa
     1801 ctcggtgagg atctcgttcg agccgttcgc ggtccacgtg ccctcgaagg ggtgaccgtg
     1861 gtgcgccgcg acctcctcgc gcaggcgggt catctgcccg tccgggtacc ggttcagggg
     1921 cagatcgcgg atcgcaccgg ccagcgcatc ggagatgtct tgtggcaacg ggtgtggaca
     1981 ctcgttggtg ttgagccgcg ccaccacctc cagctgcgga gacaggtagg gctcgacgtc
     2041 gcgaaccccg gggcgcggct gggccgcggg gctcacggtt ccaccacttc ggtggcgtcg
     2101 gcgcgcgcgt tcatggcgcg ggcgtgaccc accagaccct cggcctccgc gatggccgag
     2161 gtgccgggcg cgaaccgggt cgccgcgccg gcgtccatct gtacgaacga ggagaccgtc
     2221 acgtagtcgg ctgcccgcag gccgctcgag aaccgggcgg tccccgcggt gggaaggacg
     2281 tggttcgagg cgacgccgta gtcgccgaac ggcaccgccg tccatcggcc caggaacacg
     2341 gcgccggcgt tgcgcacacc ctcgaggaac acctcgggat cgggcaacag gatctgcagg
     2401 tgttcggccg cgaggtcgtt gacgacgtcg gtggcctgat cgaggtcacg caccaggacg
     2461 gccttcgtgt agcgcaaggc cgcatcgacg atctcgcgcc gcccggcgtc ctccagggcc
     2521 tcttcgagcg cctcgcccac ccgctcgatc agctcgggat cgggggagac gaagaacgtc
     2581 cgcgcttccg ggtcgtgttc ggcttgggcg atcagatcca gggcggccat gtcgatgtcg
     2641 atgtcgccgg ccgccacgat cgtgagctcc gacggcccgg cgagcccgtc gatgccgacc
     2701 gcgccggcca cctggcgttt ggcttcagtg acgtaggcgt tcccaggacc cacgatgcgg
     2761 tcgacgcgtg ggatcgattc ggtgccgtag gccatggcgc ccaccgcctg ggcgccaccg
     2821 gtcttggcga cgtaggtggc gcccgcgcgc atcgcggcgt acagcacggg gtcggggatg
     2881 gaaccgtccg cctggggcgg cgtgcagagg acgatctcct cgacgcccgc caccaccgcg
     2941 ggcaccacgg tcatgcacac cgatgacgga tacacggcgc gacctccggg cacgtagcac
     3001 cccacgctct cgagcgggcg cacgatctcg ccgaagcgca cgccgtcgcg ttcgtcgacc
     3061 catccgcgcg ggagctgacg cgcagccaga tccttcagcc gttcgatcag ggcgtcgagc
     3121 gcggcgcgca ggtcgggcgg cgtgtcggcc tcggcatcct tgaactcggc ctcggtgacg
     3181 aggacgccgg tctcggagat gtcgcagccg tcgaaccgct gcgccagttc gatcaggacg
     3241 tcgtcgccct ccacgaagac acgctgaagg atgccggtga cggtgtcggc gacggtgggg
     3301 tcggtctcga gccgggtggc ttcgagacgc tcgccccgct cacgcaggtc gaggaactga
     3361 agcactcagg actcctcagg gccctcggga tggaccgcgg cgacgcggct gagccgacgg
     3421 tcacgaacca gcagcaccca tccgctcacg cccaccgtga gtgcggcgag ggaaagcatg
     3481 acgacggcga tgagcaacgg ggtttcgcgg tccatggagg ggttcagggt accgaaacac
     3541 cgcccaggtc agccctggcg ccgtttcagc cgtcgtcgcg cacgatgcag aactcgttgc
     3601 cctcggggtc ggccaggacg atgaacggat cctcgggatc gtcgctggcc cagcgacgcg
     3661 tggcgccgat ggcctcgatc gcgctcgcgg tggccgcttc atccttcgcg gcgaagtcga
     3721 catgcaggcg attcttgccg gccttggatt ccgggacacg ttggaaggcg atccgcgtgc
     3781 cgttgtcgtg tgagcggatc gagacccagt cgtcgtgggc gctaccgatc tcgaaccccg
     3841 tgaaccgcat ccagaacgcg gcgagcgaac gtggatccgc gcagtcgatc accaattggt
     3901 agaagcggac gtcgatgtcg gccatggtcg agcacctcct tgcgtgctcg accctaccgt
     3961 cgggcgtcca ccccggacat ggaggcgggg ggg
//
LOCUS       DATDBZ010000156         2357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1485655, whole genome shotgun sequence.
ACCESSION   DATDBZ010000156 DATDBZ010000000
VERSION     DATDBZ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485655"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VIX62_03825"
     CDS             <1..561
                     /locus_tag="VIX62_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEY5859390.1"
                     /translation="SGLGAFKGSLVVFDTGGGSTQFTFGHGAEVDDRFSVEVGAVRYT
                     ERFGLDTAVSIETLRAAIEAIASDLTRLEGMPAPAALVGMGGAMTNITAVKLGLAPYD
                     PDAVHGATLERADIDRQIEMYRTRDLEDRRTIVGLQPKRADVILAGACIVRTVMALLE
                     QHTVTVSDRGLRHGLLRERFGPSARQ"
     gene            complement(587..2350)
                     /gene="sulP"
                     /locus_tag="VIX62_03830"
     CDS             complement(587..2350)
                     /gene="sulP"
                     /locus_tag="VIX62_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEY5859391.1"
                     /translation="MPSIVAVDGDGAGGRDRWHLVPIVGWLGSYQRRWLRGDLIAGFA
                     VAALIVPKNLGYAGIAGIPLQNGLYAAAAGAILYPIFGTSRQISTGPSSGLAAVAASA
                     VAVAGLTGTADVASFVALMTLTSGALFLVLAVLRMGWIAQFLSRAVVTGFLFGAAIDV
                     VIGELPKLTGTEVTGSNSLRELGSWVRTLGDTQSATVVVGAAALAVVFGLRFVAPSIP
                     GALVLVIGGLLASRLFDLAARGVALVGDVPRGLPTFDVPDIDVVGDHAGTVAAAAIAL
                     VLIGFSQTAGDSRAFAARHRYRIDINQESVAQGMANVGAGLVQGMPVSTSLSASSLND
                     HTGARTGLASITSGFIVLLTLLFLAPLFSDLPKPVLAALIIEAVVMGMMDVPEMRRLL
                     RVQRFDFWIAVAAITGTLLLGVLAGVMVGIGLSLIWLISVATRPPMPVLARDPQTKYF
                     RELAENPGDEQVPGLVVLRLDGGLFFATSDALEDRVREVALSTPDIRGIVLDFVAVDF
                     IDSQGSAKMNEILALTEQAGVTLRLARVKPSVRELLRREGVLERLGDEADYLSIAQAV
                     EAHTSSDEHEPGRRVPDVERS"
BASE COUNT          448 a          815 c          780 g          314 t
ORIGIN      
        1 agcggcctgg gcgccttcaa gggttcgctg gtcgtgttcg acaccggagg cggcagcacc
       61 cagttcacgt tcggtcacgg cgccgaggtc gatgaccgct tcagcgtcga ggtcggcgcg
      121 gtgcgctaca ccgagcgttt cgggctggac acggcggtgt cgatagagac cttgcgcgcc
      181 gccatcgaag cgatcgcctc agatctcacc cggttggagg ggatgccggc gcccgccgcg
      241 ctcgtcggca tgggcggtgc gatgaccaac atcaccgcgg tgaagctcgg cctcgccccc
      301 tacgaccccg acgccgtaca cggggcgacc ctggagcgag ctgacatcga ccgccagatc
      361 gagatgtacc ggacccgcga tctcgaggac cgtcgcacga tcgtcgggct ccagcccaag
      421 cgagcagacg tgattctcgc cggggcatgc atcgtgcgta ccgtgatggc actgctggag
      481 cagcacacgg tgacggtgag cgaccgcggc ctgcgacacg gtcttctccg cgagcgcttc
      541 gggccgtcgg cccggcagtg aaagcggtgc gcccgaccgg ccctgctcag gatcgctcaa
      601 cgtcaggtac gcggcgtccg ggctcgtgct cgtccgatga ggtgtgtgcc tcgaccgctt
      661 gcgcaatgct gagatagtcg gcctcgtcac ccaggcgctc gagaacaccc tcacgccgga
      721 gcagttcacg gacggagggt ttcacgcggg ccaggcgaag cgtcacgccg gcttgctcgg
      781 tgagggcgag gatctcgttc atcttggccg aaccctggga gtcgatgaag tcgacggcaa
      841 cgaagtccag cacgatcccg cggatgtccg gcgtcgacaa ggcgacctcc cggacgcggt
      901 cctcgagggc gtcggaggtt gcaaagaaca ggccgccgtc caatcggagc accaccaggc
      961 ccgggacttg ctcgtcgccc gggttctcag ccagctcgcg gaagtacttc gtctgcgggt
     1021 cccgcgccaa cactggcatc ggaggacgcg tcgccaccga gatcagccag atcaacgaca
     1081 gtccgatgcc gaccatcacc cctgcgagca cgccgagcag gagggtcccg gtgatcgctg
     1141 ccacggcgat ccagaagtcg aagcgctgca cccgaagtag gcgtcgcatc tcggggacgt
     1201 ccatcattcc catcaccacc gcctcgatga tcaacgccgc gagcaccggc ttgggcaggt
     1261 ccgagaacag tggcgcgaga aagaggaggg tgagcagaac gatgaagccg gaggtgatcg
     1321 aggcgaggcc cgttcgtgcc ccggtgtggt cgttcaacga gctcgccgac aagctcgtcg
     1381 aaacgggcat cccctgcacg aggccagcac cgacgttcgc catgccctgg gcaaccgatt
     1441 cctggttgat gtcgatgcga tagcggtgcc gggcagcgaa cgccctggaa tcaccagccg
     1501 tttgggagaa accgatcagg acgagcgcga tggcggccgc ggcgacggtc ccggcatggt
     1561 cgccgaccac gtcgatgtcg gggacatcga acgtcggaag gcctcgagga acatcgccca
     1621 ccagagcgac cccgcgcgca gcgagatcga acagccggga cgccagcaga ccgccgatca
     1681 ccaagacgag ggcacccggg atcgagggtg ccacgaacct cagtccgaac acgacggcca
     1741 gcgcagcagc gccgaccacc accgtcgccg actgagtgtc gccgagggtc cggacccacg
     1801 acccgagctc acgcagcgag ttcgagccag tcacctcggt gccggtgagc ttcggcagct
     1861 caccgatcac gacgtcgatc gccgctccga acaggaaccc cgtgaccacc gcccgcgaca
     1921 ggaattgggc gatccacccc atcctcagca ctgcaagcac caggaagagg gcaccagagg
     1981 tcagcgtcat gagggcgacg aaggacgcca cgtcagcagt ccccgtgagg ccggcgaccg
     2041 cgaccgcgct cgcagcgacc gccgccaacc cggaactcgg accggtcgag atctgacggc
     2101 tcgtcccgaa gatcgggtag aggatcgcac ccgccgccgc ggcgtagagg ccattctgca
     2161 gggggatccc cgcgatgccc gcgtacccga ggttcttagg cacgatcagc gcggcgacgg
     2221 cgaacccggc gatcaggtcg ccgcgcagcc agcgccgttg atacgagccg agccagccga
     2281 cgatcggcac caggtgccac cggtcccggc caccagctcc atccccatcg acagccacaa
     2341 tcgacggcac cgtacac
//
LOCUS       DATDBZ010000157         2928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1055509, whole genome shotgun sequence.
ACCESSION   DATDBZ010000157 DATDBZ010000000
VERSION     DATDBZ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055509"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1612
                     /locus_tag="VIX62_03835"
     CDS             2..1612
                     /locus_tag="VIX62_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5859392.1"
                     /translation="MGERAYDAVIVGGGHNGLVAGFALSKAGLRTVVLERRPFVGGAC
                     NTEEFAPGYRASTGAYVLSMLRESIWRDMQLVRRGIVVDPAGPTLNLFPDGAHYYLGD
                     DMAANVAETKRFSPADARALPRFEDDLATLVQGVLPSFDWTAPDPRIRSLGDLRELAR
                     WGRVGLKQRKHLADLAFLFSTSATQLLSERFTDEHVMAAMGWHAINDSVAGPSTPGTA
                     YVLLHDHASEEVDGGVRQWGFVRGGMGVLTARMADAARDAGCEIRLDAEVEQITTRGG
                     RATGVRLASGEEIRGTCVLSNADPKRTFLSLCDRTELPADFVAKIEAYRCMGTSIKIN
                     LAVSELPYVRGLPEGGVQPYHRGIMEMNPFIADMDFQQAQAVQGIPADPAHIEVCFPT
                     VHDPSLAPEGKHIMTIDVNSQPYTLRDEAWDDIKESRADRAIAQMAEHFPKLPDLIEH
                     RQVVTPLDMERLMGLTGGHALHGDMAPDQLLFLRPVRGWADYRTPIAGLYLCGAGTHP
                     GGGVTGANGRNAAREVLKDVGHRGRRKG"
     gene            complement(1609..2901)
                     /gene="efeB"
                     /locus_tag="VIX62_03840"
     CDS             complement(1609..2901)
                     /gene="efeB"
                     /locus_tag="VIX62_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HEY5859393.1"
                     /translation="MTDAHGDPPPAPPDSAAISRRRFLTSVGAGVGAAAAVGGLGGYA
                     LATEKDDRSPGAGDLVVPFHGRHQAGIVTPAPAHMAFASFDVMPTVDRDELRAMLASW
                     TAAAASMCAGEPIGATDDGPRLAPPVDTGEAVGLPAAGLTVTFGVGPSLFTGELGRRL
                     GLRDRRPAELIDIPSFPGDVLHPGRSDGDLGIQVCAEDPQVVIHAVRNLVRIGRGTVL
                     SRWIQQGFLPASHDETPRNLMGFKDGTNNPKLSDATAMDATVWVPDDEPQAWMRGGTY
                     LVFRRIRILVEFWDRTNLDEQERTIGRHKGTGAPMGADAEFDPLPLDAKDSDGEPAIP
                     IDAHVRLAAASENGGAAFLRRGYSFTDGLDPVTGEIDGGLLFIAMQRDPEEQFVRVQR
                     SLAGVDALNEYIKHVGSAIFAVPGGVAPGGSVGDLLLA"
BASE COUNT          466 a          985 c         1018 g          459 t
ORIGIN      
        1 cgtgggagaa cgtgcgtacg acgcggtcat cgtcggtggt ggacacaacg gcctcgtggc
       61 cggattcgcg ctctccaaag cgggacttcg caccgtggta ctcgagcgca ggccgttcgt
      121 ggggggtgcc tgcaacaccg aggagttcgc gcctggctac cgcgcgtcga ccggcgccta
      181 tgtgctgtcg atgctgcgtg agtcgatctg gcgcgacatg caactggtgc gccgaggcat
      241 cgtcgtcgac cccgccggcc ccaccctcaa cctcttcccc gacggcgccc actactactt
      301 gggcgatgac atggcggcca acgtcgcaga gacgaagcgg ttctctcccg ccgacgcccg
      361 cgccctgccc cggttcgaag acgaccttgc aaccctggtg caaggcgtgc tgccgtcctt
      421 cgattggacc gcacccgacc cacggatccg gtcgctgggc gatctgcgtg agctcgcgag
      481 atggggccgc gtcgggctga agcagcgcaa gcatctggcc gacctcgcgt tcctgttctc
      541 caccagcgcc acgcagctgt tgtccgaacg gttcaccgac gagcacgtga tggcggccat
      601 gggctggcat gccatcaacg acagcgtcgc agggccatcg acgccgggga ccgcctacgt
      661 cttgttgcac gaccacgcca gcgaagaggt ggacgggggc gtgcggcagt gggggttcgt
      721 tcgaggcggc atgggcgtcc tgacggcgag gatggccgat gcggcgcgcg acgccggttg
      781 cgagatccgc ctcgacgccg aggtcgagca gatcacgacc cgcgggggtc gcgcgacggg
      841 ggtgcggctg gcttcgggcg aagagatccg cgggacctgc gtgctgtcca acgccgaccc
      901 caagcggacg ttcctgtcgc tctgcgatcg cacggaactc cctgcggatt tcgtcgcgaa
      961 gatcgaggcg taccggtgca tgggcaccag catcaagatc aacctcgcgg tgagcgagct
     1021 tccgtacgtt cgcgggttgc cggagggtgg cgtgcagccg taccaccgcg gcatcatgga
     1081 gatgaacccg ttcatcgccg acatggactt ccagcaggca caggcggtgc agggtatccc
     1141 cgctgacccc gcgcacatcg aggtgtgctt ccccaccgtg cacgatcctt cgctggcgcc
     1201 cgagggcaag cacatcatga cgatcgacgt gaactcccag ccgtacacgc ttcgcgacga
     1261 ggcgtgggac gacatcaagg agtcacgcgc cgaccgcgcg atcgctcaga tggcggagca
     1321 tttcccgaag ctgcccgacc tgatcgagca ccgacaggtc gtgactccgc tcgacatgga
     1381 acggctcatg ggattgaccg gcgggcacgc cctgcacggt gatatggcac ccgaccaact
     1441 gttgttcctg aggccggtgc ggggctgggc cgactaccgg acgccgatcg ccgggttgta
     1501 tctgtgtggg gcgggcacgc acccgggcgg tggggtcacc ggggcgaacg ggcgcaacgc
     1561 cgcccgggaa gtcctcaagg atgtcggcca ccgaggccgg cgcaagggct aagccagcag
     1621 gagatcgccg acagatccgc ctggcgcgac accgcctggg accgcgaaga tcgcgctgcc
     1681 gacgtgcttg atgtactcgt tgagcgcgtc gacgccggcg agcgatcgct gcacccgcac
     1741 gaactgttct tcaggatcgc gctgcatcgc gatgaacaac aggccgccgt cgatctcacc
     1801 cgtgacgggg tcgagtccgt cggtgaacga gtagccgcgc cgtaggaacg cggcgccgcc
     1861 gttctccgat gcagctgcca gccggacgtg ggcatcgatc gggatcgccg gctcaccgtc
     1921 gctgtccttg gcgtccaggg gcaacggatc gaactcggcg tcggcaccca tcggcgcgcc
     1981 cgtgcccttg tgtcttccga tcgtgcgttc ctgctcgtcc aggttggtgc gatcccagaa
     2041 ctcgaccagg atccggatcc gcctgaagac gaggtacgtg ccaccgcgca tccacgcctg
     2101 cggctcgtcg tcgggaaccc agacggtggc atccatcgct gtcgcgtcgc tgagtttggg
     2161 gttgttggtg ccgtccttga atcccatcag gttgcgcggg gtttcgtcgt gggacgcggg
     2221 caggaacccc tgctggatcc accgcgagag caccgtgcct cgtccgatcc tcaccaggtt
     2281 gcgcacggcg tggataacga cctgtggatc ctccgcgcag acctggatcc cgagatcgcc
     2341 gtcggatcgc cccgggtgga ggacgtcgcc ggggaacgag gggatgtcga tgagttcggc
     2401 cggtcgccga tcccggaggc cgagccgtct ccccagctcg ccggtgaata gtgacggccc
     2461 cacgccgaag gtgaccgtga gtccggcggc cggcaacccc accgcttcac cggtatcgac
     2521 cggcggagcg aggcgcggtc catcgtcggt ggcaccgatc ggttcaccgg cgcacatcga
     2581 cgccgcagcc gccgtccatg acgccagcat ggcgcggagt tcgtcgcgat cgaccgtggg
     2641 catcacgtcg aaggacgcga acgccatgtg ggccggcgcc ggtgtcacga tcccggcctg
     2701 gtgccgtcca tggaacggca cgacgagatc gcccgcgccg ggggagcggt cgtccttctc
     2761 ggtcgccagg gcgtagccgc ccagcccacc gacggcggcc gccgcgccga cgccggctcc
     2821 gacagaagtc aggaaccggc gacgggagat cgcggcggag tctgggggcg cggggggcgg
     2881 gtccccgtgt gcatcggtca tgtcactacg acgatagccg cgacttgc
//
LOCUS       DATDBZ010000158         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2101556, whole genome shotgun sequence.
ACCESSION   DATDBZ010000158 DATDBZ010000000
VERSION     DATDBZ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101556"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VIX62_03845"
     CDS             <1..379
                     /locus_tag="VIX62_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859394.1"
                     /translation="KPTVVSATGTYLDIPEDCVLRVAAGPADPSELALRLRTLLEDDE
                     LREHMGAAAAAHMRELRDGEATAHGYEHAIERTLELVRDPGRKAMAIWGKSLVDVGIS
                     EAMVAEGYGMEYARALESFKTSP"
     gene            429..1367
                     /locus_tag="VIX62_03850"
     CDS             429..1367
                     /locus_tag="VIX62_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859395.1"
                     /translation="MTSGFIARPHRTQEPTIAASTFVERTSEAPSLHVRTRLRELWAY
                     REILLNLVRKELKVKYTASVLGALWSVLNPIVFLAVFSFVTKVLNNQIPDFPVFLLSG
                     LLAWNLFNASLLQGSRSVLDNANLVKKVAFPREILPLSAIGVAAFDFVLQSAVLLLFI
                     VVSGHGFSATVLLLYPASIVTLLVLSTALVFWVAALNVRYRDVGHLLNLGLLVWFWMT
                     PIVYQAGLVQSYFANRGTPGLWNLFLLNPLTPVVLGFQRALYGAPVQDGVLVLPDVSV
                     AWQLGVLGIVFVASLALLALTWRLFFRMSGDFAEEL"
     gene            1364..>2047
                     /locus_tag="VIX62_03855"
     CDS             1364..>2047
                     /locus_tag="VIX62_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5859396.1"
                     /translation="MTTAIEVQDVSKSFRLYRERPSSVKQRLLVTRSKAEDFWALREV
                     SFDIEQGSSVGLIGHNGSGKTTLLKCIAGILRPTTGSIRLRGRMAALLELGAGFHPEL
                     TGRENVYLNASFLGLSRRDTDRVYDDIVGFAELEDFMDNQVKFYSSGMLVRLGFAVAV
                     HVDPEVLLIDEVLAVGDESFQQRCIDRVRRFQADGRTIVLVTHALDTVVDVCDRAVML
                     DHGLVHMDGA"
BASE COUNT          299 a          670 c          703 g          375 t
ORIGIN      
        1 taagccgacg gtggtgagcg ccaccggcac ctacctggac atccccgagg actgcgtgct
       61 gcgtgtcgcc gccggcccgg ccgatccgtc ggaactcgcc ctgcggctgc gcacgctgct
      121 ggaggacgac gagctccgcg agcacatggg ggcggccgcg gccgcgcaca tgcgtgagct
      181 ccgcgacggt gaggccaccg cccacggcta cgagcacgcg atcgagcgga cgctcgagct
      241 cgtccgagac ccgggaagga aggcgatggc gatctggggc aagtccctcg tcgacgtcgg
      301 catctccgag gcgatggtgg ccgagggcta cggcatggag tacgcgcggg cgctggagag
      361 cttcaagaca tcgccatagg ggcggcaggg gcctcgagag ggcccgttgc tagactcgtc
      421 cagcccgcgt gaccagcggg ttcatcgcac gaccacaccg aacgcaggag cccacgatcg
      481 ccgcgagcac gttcgtcgaa cggacctccg aggcaccgag cctccatgtc cgcacgcgcc
      541 tgcgcgaact gtgggcctat cgcgagatcc tgctgaacct ggtccgcaag gaactgaagg
      601 tcaagtacac ggcgtcggtg ctcggcgcgc tgtggtcggt gctgaacccg atcgtgttcc
      661 tggcggtgtt ctcgttcgtt acgaaggtcc tcaacaacca gatccccgac ttccccgtgt
      721 tcctgttgtc gggcctgctc gcgtggaatc tgttcaacgc ctcgctgctg cagggttcgc
      781 gatccgtgct cgacaacgcc aacctcgtga agaaggtcgc gttcccgcgc gagatcctgc
      841 cgttgtcggc gatcggggtg gccgccttcg acttcgtgtt gcagtcggcg gtgctgctgc
      901 tgttcatcgt ggtgtcgggc cacgggttct cggcgaccgt cctgctgctc tatccagcgt
      961 ccatcgtgac gttgctggtg ctgtccaccg cgctggtgtt ctgggtcgcg gcgctcaacg
     1021 tgcgctatcg cgacgtgggt cacctgctga acctcgggct gctggtgtgg ttctggatga
     1081 cgccgatcgt ctaccaggca ggtctcgtgc agtcctactt cgcgaaccga ggcacgcccg
     1141 gcctgtggaa cctgttcctg ctgaaccccc tcacaccggt ggtgctgggc ttccagcggg
     1201 cgctctacgg cgcgccggtg caggacggcg ttctggtgct cccggacgtc agcgtcgcgt
     1261 ggcagctcgg ggtactcggc atcgtgttcg tcgcgagcct cgcgctgctg gcgctcacct
     1321 ggcgactgtt cttccggatg tccggcgatt tcgcggagga actgtgacca cggcgatcga
     1381 ggtccaggac gtctccaagt ccttccggct ctaccgggag cgaccgagca gcgtgaagca
     1441 gcgcctgctg gtgacgcgct ccaaggccga agacttctgg gccctgcgcg aggtctcctt
     1501 cgacatcgag cagggctcgt cggtgggact gatcggacac aacgggtcgg gcaagaccac
     1561 gttgctgaag tgcatcgccg gcatcctgcg ccccaccacc ggctcgatcc ggctgcgagg
     1621 tcggatggcg gcgctgctcg agctgggcgc ggggttccac ccggagctca ccggccggga
     1681 gaacgtctac ctcaacgcat cgttcctggg gctctcgagg cgcgacaccg atcgcgtcta
     1741 cgacgacatc gtcgggttcg ccgaactcga ggacttcatg gacaaccagg tgaagttcta
     1801 ttcctcgggc atgctcgtgc gcctcgggtt cgcggtggcc gtccacgtcg atccggaggt
     1861 gctgttgatc gacgaggtgc tcgccgtggg ggatgagtcc ttccagcagc gatgcatcga
     1921 ccgggtgcgg cggttccagg cggacggccg cacgatcgtg ctggtgaccc acgcgctcga
     1981 caccgtcgtc gatgtgtgcg accgcgcggt gatgctcgac cacggcctgg tgcatatgga
     2041 cggcgcg
//
LOCUS       DATDBZ010000159         5241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2920504, whole genome shotgun sequence.
ACCESSION   DATDBZ010000159 DATDBZ010000000
VERSION     DATDBZ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2920504"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VIX62_03860"
     CDS             complement(<1..339)
                     /locus_tag="VIX62_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014567579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEY5859397.1"
                     /translation="MKVMFVCSSGGHLTQLYRLKPWWEQHDRRWVTFDTADATSLLAG
                     ETVAWAHYPTTRNIPNALRNLRLAWRLLRSERPDVVVSDGAGVAVPFFVIAKVLGSAT
                     VYLEVYDRIDS"
     gene            complement(359..1831)
                     /locus_tag="VIX62_03865"
     CDS             complement(359..1831)
                     /locus_tag="VIX62_03865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY5859398.1"
                     /translation="MQGRIEDMGAADEPRSYRVDDVTVIVERGTSARERYTHLYRLIA
                     LTDVLSIAVALLLAYWVRFGIEVPSGDFLLLLAGAPFAMLVVYGMFHLYDAYRYTAAE
                     EFRRILLAVSLGVGGILALSFWSKGEFSRSWLALSWAFAIVAALASRRLWHAHIGHLK
                     ADGELSFPTLIVGTNAEAVHLSTLMERPSFGFRPLGMVATKARDGDLHDLPILGSVTD
                     LREVIRQVGAECVFVAATALSIDEMKHVAKAVRLEGVEVRVTATLPEVLSSRVAVQSL
                     GGVTALSLRPVRLTGTQAALKRAFDIVVAGVGTILLSPLLITLALAVKLSTPGPVLYR
                     QPRVGHRGRPFTMLKFRTMHVGADAMVDSLREEHGVQDLMFKLQNDPRVTRVGRVMRK
                     YSLDELPQLINVIRGDMSLVGPRPPLPEEVTRYEDWQFDRLEVPPGISGLWQVSGRSD
                     LSFDDCVRLDLFYIENWSLAYDLYILAKTVPVLISQRGAY"
     gene            complement(2145..3077)
                     /locus_tag="VIX62_03870"
     CDS             complement(2145..3077)
                     /locus_tag="VIX62_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5859399.1"
                     /translation="MSEIWLVLAVGAAFFAVVLGGLAVEQSMSDKKRAVRLLESHVSA
                     NESVNLRDHQLSQNFGQRVLVPIVARAGRIARRITPLDTRDRIAKKIMLAGAPPGWDA
                     ERIMAFKVIGAVAGVGGGLALTAMLRPAPLISFVAIGLLGFVGFVVPDSTLNRRVEER
                     QKEILRTLSDTLDLLTISVEAGLSLNASIAQVVQNVPGVLSSEFARMLQEIQLGVPRS
                     EAFRHLADRTDVEELNAFALAMIQADAFGVSIASVLRTQAQQLRIKRRQWAEQKAQQT
                     PVKIVFPLVLCVLPALFVVIVGPGAIRIVTQLFK"
     gene            complement(3070..4080)
                     /locus_tag="VIX62_03875"
     CDS             complement(3070..4080)
                     /locus_tag="VIX62_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5859400.1"
                     /translation="MIGAIAASFWSSPVAQIVLAALVGGAVFALGWLMLGTAARARKD
                     REIATRVAAVTGAPRQPVSQASEGTQGWIPDRVTHFGRRFAESRGFSERLDAELEAAG
                     VKVRSGEFVVLSVVAFLVGGVIGAALMRSPLLALLIAAICGFAPTAALRAALRKRTER
                     MREQLPDVLTIMASSLRAGHSFMQALDTVAREIPQPAATEFQRVVAEIRLGRSTDDAL
                     EALSERVGSADFKWAVLAVNIQREVGGNLAEILDNVADTLRERAQMRRQIRVLTAEGR
                     LSAWVLTLLPLGIAAYMFAVNPEYIGLLFTTRMGLFMVGVAVILLVLGVFWMRKIVDI
                     DV"
     gene            complement(4077..>5241)
                     /locus_tag="VIX62_03880"
     CDS             complement(4077..>5241)
                     /locus_tag="VIX62_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY5859401.1"
                     /translation="LRELLDEEEGPLSAQEKLLIVRQIGDSVLGLGPLEPYVRDPEVT
                     EIMVNKWDTIYVERAGKLYWTGTKFHDEQQLRRTIDKIVGKVGRRVDEASPYVDARLP
                     DGSRVNAVIPPLAIDGPALTIRKFSADPYQADDLVEFGTMSSSTAKFLDACVRGRINI
                     MVSGGTGAGKTTTLNVLSSFIPDDERIITIEDAAELKLQQPHVVRLEARPPNIEGKGQ
                     VSIRDLVRNSLRMRPDRIVVGECRGPEALDMLQAMNTGHDGSISTVHSNSPRDSLSRL
                     ETITLMAGMDLSPRSVREQISSAIQLIVHQSRLKDGSRRITHVTEVSGMESDVITLQD
                     IFVFDFAAGLDEEGKFKGHMKATGLRPMFLEKMAERGVFLDAETFALDPKEGR"
BASE COUNT          916 a         1745 c         1793 g          787 t
ORIGIN      
        1 cgagtcgatg cggtcgtaca cctcgaggta gacggtggcc gagcccaaca ccttggcgat
       61 gacgaagaag ggcaccgcga cgccggcgcc gtcggacacc accacatcag ggcgctccga
      121 gcgcaggagg cgccacgcga gccggaggtt gcgcagggcg ttggggatgt tccgtgtcgt
      181 ggggtagtgc gcccacgcca ccgtctcgcc cgccagcagc gatgtggcat cggccgtgtc
      241 gaacgtcacc catcgacggt cgtgctgctc ccaccacggc ttcaagcggt acagctgcgt
      301 gaggtgccca ccggaggagc acacgaacat gaccttcaac gtggtcctcg cgtacggctc
      361 agtacgcccc gcgctgagag atcagcaccg ggaccgtctt ggccaggatg tagaggtcgt
      421 aggcgagcga ccagttctcg atgtagaaca ggtcgaggcg cacgcaatca tcgaacgaga
      481 ggtcgctgcg accgctcacc tgccacaggc cgctgatccc cggtggcacc tccagccggt
      541 cgaactgcca gtcctcgtag cgggtgacct cctcgggcag cggcggccgg ggcccgacga
      601 ggctcatgtc cccgcggatc acgttgatca gctgcggcag ttcgtcgagg ctgtacttgc
      661 gcatgacgcg acccacgcgg gtcacgcgcg ggtcattctg cagcttgaac ataaggtcct
      721 ggacaccgtg ctcttcgcgc agcgaatcga ccatggcgtc ggcacccacg tgcatcgtgc
      781 ggaacttcag catcgtgaac ggacgtccgc gatgaccgac gcggggctgg cggtagagca
      841 ccgggccggg ggtggagagc ttgacggcca gggccagggt gatgagcaac ggcgagagca
      901 ggatcgtgcc gacgccggcc acgacgatgt cgaaggcccg cttcagggcc gcctgggtgc
      961 cggtgagccg gaccggacgg agcgacagcg ccgtcacgcc gccgagcgac tggacggcga
     1021 cgcgggacga cagcacctcc ggcagggtcg ccgtgacccg cacctcgacg ccttcgagcc
     1081 gcaccgcttt ggcgacatgc ttcatctcgt cgatcgacag cgcggtggcg gccacgaaga
     1141 cgcactcggc gcccacctgg cggatcacct cgcgcagatc ggtcaccgaa ccgaggatcg
     1201 gcaggtcgtg caggtcaccg tcacgagcct tggtggcgac catgcccagc ggacggaagc
     1261 cgaaggacgg acgctccatg agcgtcgaca ggtgcaccgc ctcggcgttg gtgcccacga
     1321 tgagcgtggg gaacgacagc tcaccgtccg ccttcaggtg gccgatgtgt gcgtgccaga
     1381 gtcgccgcga ggccagggcc gccacgatgg cgaacgccca cgagagggcc agccatgaac
     1441 gggagaactc tcctttggac cagaaggaca acgccaggat gcccccgacc ccgagcgaga
     1501 ccgccaacag gatccggcgg aactcctcgg cggcggtgta ccggtaggcg tcgtacaggt
     1561 ggaacatgcc gtagacgacg agcatcgcga agggcgcacc ggccaggagc aacaggaagt
     1621 cgccactggg cacctcgatc ccgaagcgaa cccagtaggc gagcagcagc gccaccgcga
     1681 tcgacagcac gtcggtgagg gcgatcaggc ggtacaggtg ggtgtagcgc tcccgagcgg
     1741 aggtcccccg ctcgacgatg acggtgacgt cgtcgacacg gtaggagcgc ggttcatcgg
     1801 cggctcccat gtcttcgatg cgtccctgca cggtccgtcc catctcgctt caccggtccg
     1861 cgggctggtg gtcgtttcga ccgggggacg cctgttgccg acgtgacccg cagccagacc
     1921 cgcgaagggg gaagtgttcc ctcccctgtc tgggggacga taaccggtct agcacgggtg
     1981 tgctatacgg cgaaggggtc gtctttggcc tacatcacgg tcacgaagac ctaggaccga
     2041 tgggacgaga ccctggcgcg acgatcccgg ccttgcgggg ctgggatcat cggcccttcg
     2101 ccggtccagg cccaccgccg ggcacgccgg cgatcggggt cggattattt gaagagctgg
     2161 gtcacgatgc ggatggcgcc ggggcccacg atcacgacga agagggccgg gagcacgcac
     2221 aggaccagag ggaagacgat cttgaccggc gtttgctgcg ccttctgctc cgcccactga
     2281 cggcgcttga tccgcagctg ctgtgcctgg gtccgcagca cgctggcgat cgagacaccg
     2341 aacgcgtcag cctggatcat ggccagcgcg aaggcgttga gctcttcgac gtcggtgcgg
     2401 tcggcgagat ggcggaaggc ctccgagcgc ggcacgccca gctggatctc ctggagcatg
     2461 cgcgcgaact cggacgacag cacgccgggc acgttctgca ccacctgggc gatcgaggcg
     2521 ttgagcgaca gtcccgcttc gacgctgatc gtgagcaggt cgagcgtgtc cgacaaggtc
     2581 cgcaggatct ccttctggcg ctcttccacg cggcggttca gcgtggaatc cggtaccacg
     2641 aacccgacga acccgagcaa gccgatcgcc acgaacgaga tcaacggcgc gggccggagc
     2701 atggccgtca gcgcgagacc tccgcccaca ccggccacgg caccgatcac cttgaaggcc
     2761 atgatccgct cggcgtccca tccaggggga gcgccggcga gcatgatctt cttcgcgatc
     2821 cgatcgcggg tgtcgagcgg ggtgatcctt ctcgcgatgc gccccgctcg tgccacgatc
     2881 ggcacgagca cccgctgacc gaagttctgg gagagctgat ggtcgcggag gttgaccgac
     2941 tcgttcgcgc tgacgtggga ctcgaggagc cgcacggcgc gcttcttgtc ggacatgctc
     3001 tgttcgacgg ccaggcctcc aagcacgacg gcgaagaagg cggccccaac ggcgagtacg
     3061 agccagatct cagacatcga tatccacgat cttcctcatc cagaagaccc cgagcaccag
     3121 caggatgacc gcgacgccga ccatgaacag acccatgcgg gtcgtgaaca acaggccgat
     3181 gtactcggga ttgacggcga acatgtaggc cgcgatgccg agcggcagca gggtgagcac
     3241 ccaggccgac aggcggccct cggcggtgag cacccggatc tgccgacgca tctgcgcccg
     3301 ttctcggagc gtgtccgcga cgttgtcgag gatctcggcg aggttgccgc cgacctcacg
     3361 ctggatgttg acggccagga ccgcccactt gaagtccgca ctgccgacgc gctcggagag
     3421 cgcctccaag gcgtcgtcgg tggagcggcc cagacggatc tcggcgacca cgcgctggaa
     3481 ctcggtcgcg gccggctgcg ggatctcccg cgcgaccgtg tcgagggcct gcatgaagct
     3541 gtggcccgct cgcagagagc tcgccatgat cgtcaggacg tcgggcagct gctcgcgcat
     3601 gcgctcggtg cgtttgcgga gcgccgcgcg gagcgcggcc gtgggcgcga acccgcagat
     3661 ggcggcgatc agcagcgcca ggagcgggct ccgcatcagc gccgcgccga tcacgccgcc
     3721 gacgaggaag gccaccaccg acagcaccac gaattcaccc gagcggacct tgaccccggc
     3781 cgcctccaat tcggcatcca gccgttcgct gaacccacgg gattcggcga accggcgacc
     3841 gaagtgcgtg acgcggtcgg ggatccagcc ctgcgtgccc tcggaggcct gcgacaccgg
     3901 ctgccgcggg gcgccggtga cggcggccac gcgggtcgcg atctcgcggt ccttcctggc
     3961 gcgagcggcg gtaccgagca tgagccaccc cagcgcgaag accgcgccac ccaccagggc
     4021 ggccagcacg atctgggcga ccggcgaact ccagaacgat gcggcgatcg cgccgatcat
     4081 cggccctcct tcgggtcgag ggcgaaggtc tcagcgtcga ggaagacacc gcgctccgcc
     4141 atcttctcca ggaacatggg ccgcaggccg gtggccttca tgtggccctt gaacttgccc
     4201 tcttcatcca gccccgcggc gaagtcgaac acgaagatgt cctgcaaggt gatcacgtcg
     4261 ctctccatgc ccgaaacctc ggtgacgtgc gtgatacggc gcgaaccgtc cttgagccgc
     4321 gactggtgga cgatcaattg gatcgccgag ctgatctgct cacgcaccga cctcggcgac
     4381 aggtccatgc cggccatcaa cgtgatcgtc tcgagcctgc tgagcgagtc gcgcggcgag
     4441 ttcgagtgga ccgtgctgat cgaaccgtcg tgaccggtgt tcatggcctg gagcatgtcg
     4501 agggcctcgg gaccacgaca ctcaccgacg acgatgcggt cgggccgcat acgcagcgag
     4561 ttgcggacca ggtcgcggat cgagacctgc cccttgccct cgatgttggg cgggcgagcc
     4621 tcgaggcgga ccacgtgcgg ctgctgcaac ttgagctcgg ccgcgtcctc gatcgtgatg
     4681 atgcgctcgt catcggggat gaacgagctc aacacgttca aggtggtcgt cttgccggcg
     4741 ccggttcctc cggacaccat gatgttgatg cggccgcgga cgcaggcgtc caggaacttg
     4801 gcggtcgacg agctcatcgt gccgaactcc accaggtcgt cggcctggta ggggtcggcg
     4861 gagaatttcc ggatcgtcag ggcgggaccg tcgatcgcga gcggcgggat caccgcgttg
     4921 acacgcgaac cgtcgggcag acgggcgtca acgtacgggc tggcctcgtc gacgcgacgg
     4981 ccgaccttgc cgacgatctt gtcgatcgtg cgacgcagct gctgctcgtc gtggaacttc
     5041 gtgccggtcc agtacagctt gccggcgcgc tcgacgtaga tcgtgtccca cttgttcacc
     5101 atgatctcgg tgacttccgg gtcacgcacg tatggctcca gcggtcccaa gcccagcacg
     5161 ctgtcgccga tctggcgaac gatcagcagc ttctcttgcg cggagagcgg gccttcctcc
     5221 tcatcgagga gctcgcgcag g
//
LOCUS       DATDBZ010000160         4864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_262067, whole genome shotgun sequence.
ACCESSION   DATDBZ010000160 DATDBZ010000000
VERSION     DATDBZ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262067"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1413)
                     /locus_tag="VIX62_03885"
     CDS             complement(187..1413)
                     /locus_tag="VIX62_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859402.1"
                     /translation="MNGARQGWLVAVREMRERSRTRGFRAGIVVTILIVVAVIVVPAM
                     LGSGGGPNDVGLAGSVPDGLPIAIRAQSDAAGMEARIHRYETVGGGEEAARQGDVDVL
                     VVDARRLEWPRQTDEQLRAVVTGAIQLVAVRERAAAAGISPGDLLAVVAPVRIENVQL
                     GALPGGSADDEMAAMLMTVLLLIAISTYGNMVLTGVVEEKASRVVEVLLVRMSARTLL
                     AGKVVGIGLLGLAQFVVTALVTFAAMTAVASVDVTAVRGIVLAWVVAWFLLGYALYAM
                     AYGALGSLASRTEDGQSVAGPVIAVLIAAYWVSFAALGQDPQSGASRLVSLFPATAPF
                     AMPARIAMGASTWWEPMLAVILTLGAIAGLVRFGGRVYTGAILHAGPTLKVRDAWRGA
                     TSPHGSGTGPAFLPRA"
     gene            complement(1410..2297)
                     /locus_tag="VIX62_03890"
     CDS             complement(1410..2297)
                     /locus_tag="VIX62_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5859403.1"
                     /translation="MLRFDAVVKRFGSVTALDGCTFVARPGRLTGFLGPNGAGKTTAM
                     RAVFGLADLDAGTVSWRGTPIAPMDRPRFGYMPEERGLYPRMRVRDQLTYLGRLCGRT
                     ADDAIRSVDMWLERLGLADRASDPLDALSHGNQQRVQLIAALINEPVLLVLDEPFSGL
                     DPIAIAHMGELLTEVATAGATVLFSSHQLDLVEDLCEDVVIIDHGRIVLSGELTELRE
                     AVPQRFVDIRFRGAVPDWSELAGVEVVESGEGLARLQVDRDVDLTEVVARAPRTTDIV
                     SFAYQPPTLSELFRGAVAA"
     gene            2416..2700
                     /locus_tag="VIX62_03895"
     CDS             2416..2700
                     /locus_tag="VIX62_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859404.1"
                     /translation="MSDEELLAKAEAALTEIKMSQGLSDHHASVLAALRIRLTGSAGE
                     SLDELLAAAGDIGPDDRLQEEIEKAQAAKARKPSLDDLLTQEKPKRDWPG"
     gene            complement(3040..3873)
                     /locus_tag="VIX62_03900"
     CDS             complement(3040..3873)
                     /locus_tag="VIX62_03900"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY5859405.1"
                     /translation="MRMKLVALLAAVALVAGGYVVGQAGARRGGDDSTTLPGFSEVVS
                     LSHVNAPARTPLFPGDPAFTLTTVATVEDDGFYMQYVREGEHTGTHYSAPCHFRDGKP
                     CAEDLDAADYVLPAVVVDVRDAVAADANHVVTKTDLQDWETGHGEMPSGAAVLLWTGC
                     DAFWGPTLAKDEPTFYNCGQPGAKYSQPGFSKGAVRWLIETGVLAKRGALGTDTFGPD
                     PSADANYWESYLTLNRHRFTLENLTNLGAMPTTGGWIVIGGPMNRNGSGAASTVFGLV
                     P"
     gene            complement(3966..4463)
                     /locus_tag="VIX62_03905"
     CDS             complement(3966..4463)
                     /locus_tag="VIX62_03905"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5859406.1"
                     /translation="MSRYPLEDAFAHHAWATVRLIDACTVLTPERLAAAVPGTYGSIL
                     DTMRHLVGADSWYLFDLTGDAARCIEEGEMDLGHLRAAMQDDGEAWLRLLGDHPDPDT
                     VVHEVDESDGFERDAPIGIRLAQALHHGTDHRSQVCTALTNLGVDPPSIDVWDFGLDT
                     GRTVE"
     gene            4636..>4864
                     /locus_tag="VIX62_03910"
     CDS             4636..>4864
                     /locus_tag="VIX62_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859407.1"
                     /translation="MLVVALVVGATLGVASSAGAKIVGQRVAGNLRQAVAFTFDDRDR
                     LWVVEKQVGSIYVQKLGSRQRHRFFRIPDVYG"
BASE COUNT          773 a         1713 c         1654 g          724 t
ORIGIN      
        1 cagctgggcc gggctcacca gatggacgag cgcacgccgg gagcgctcgc gcgggccgac
       61 gcgatgttcg cgtgggaccc ctccccctgg ttcgcgttcg tgtactgacg tctctcagcg
      121 cgatgcacag gccgtccgct gcgctcctct tactgggaac ccgccgactg aggctcgtcg
      181 aagagcctag gcccgtggga ggaacgccgg gccggttccg ctgccatgcg gcgacgtcgc
      241 gccgcgccac gcgtcgcgca ccttgagcgt cggtccggcg tggaggatcg cgccggtgta
      301 gacacgcccc ccgaaccgca cgagaccggc gatggcaccg agggtgagga tgacggcgag
      361 catcggttcc caccacgtgg acgcacccat cgcgatgcgg gcgggcatcg cgaacggtgc
      421 ggtcgccggg aagagcgaga ccaaccggga tgcgccactc tgtgggtcct gaccgagtgc
      481 agcgaacgag acccagtagg cggcgatcag cacggcgatc accggtccgg caacgctctg
      541 gccgtcctcg gtccgcgaag cgagggagcc gagcgccccg tacgccatgg cgtagagggc
      601 gtagccgagc aggaaccacg cgacgaccca cgccagcacg atgccgcgga cagccgtgac
      661 gtcaacggag gcgaccgccg tcatggccgc gaaggtgacc agggcggtca cgacgaactg
      721 cgcgagcccg agcagaccga tcccgacgac cttgcccgcg agcagcgtgc gggcggacat
      781 ccggacgagc agcacctcga cgacgcggct cgccttctcc tccaccacac cggtcagcac
      841 catgttcccg tacgtgctga tcgcgatcag cagtaggacg gtcatcaaca tggccgccat
      901 ctcgtcgtca gcgcttcccc cgggcagcgc gccgagctgg acgttctcga tccggaccgg
      961 tgcgacgacg gcgagcaggt cgccggggct gataccggcg gcggccgctc gctcgcggac
     1021 cgcgacgagc tggatggccc ctgtgacgac ggcacggagc tgctcgtcgg tctggcgtgg
     1081 ccactcgagc cgccgtgcgt cgaccacgag cacgtcgacg tcgccctggc gcgccgcctc
     1141 ctccccgcct cccacggtct cgtatcggtg gatcctcgcc tccataccgg cagcgtcgct
     1201 ctgggctcgg atcgcgatgg gcagtccgtc ggggacagag ccggccagcc cgacgtcgtt
     1261 cgggccgccg ccgctcccga gcatggccgg cacgacgatc accgcgacca cgatgaggat
     1321 cgtcaccacg atccctgcgc ggaaaccacg cgtgcggctg cgttcgcgca tctctctgac
     1381 ggcgacgagc cacccttgcc gcgcgccgtt catgccgcca ccgccccgcg gaacagctcc
     1441 gacagcgtcg ggggctggta ggcgaacgaa acgatgtcgg tggttcgtgg agcgcgggct
     1501 accacctcgg tcaggtcgac gtcacggtcg acctggagac gggccagccc ctcccccgac
     1561 tcgacgacct ccacgccggc cagctccgac caatcgggta cggcgccgcg gaatcggatg
     1621 tccacgaacc gctgcggcac cgcttcccgc agctctgtga gctcgcccga caggacgatg
     1681 cggccgtggt cgatgatgac gacgtcttcg cagaggtcct cgaccaggtc cagctggtgg
     1741 ctggagaaca gcaccgtcgc accggcggtc gcgacctcgg tcagcagctc gcccatgtga
     1801 gcgatcgcga tcggatccag tccggagaac ggctcatcga gcacgagcaa cacgggctcg
     1861 ttgatgaggg cagcgatcag ctgcacccgc tgctggttgc cgtgggagag ggcgtcgagg
     1921 gggtcgctcg ctcggtcggc cagaccgagt cgctcgagcc acatatccac gctgcggatc
     1981 gcgtcatctg ccgtccgtcc gcagagccgg ccgagatacg tcagctggtc gcgcacccgc
     2041 atgcggggat acaggcctcg ttcctcgggc atgtagccga accgcgggcg gtccatcggt
     2101 gcgatgggcg tgcctcgcca ggacacggtg cccgcgtcga gatcggcgag tccgaatacg
     2161 gcacgcatcg cggtcgtctt gccggcaccg ttggggccga ggaagccggt gagtcgaccc
     2221 ggccgggcga cgaacgtgca cccatcgagc gccgtcacgg acccgaaccg cttcacgacc
     2281 gcatcgaacc ggagcacctt ccacctctgc ttccagcgtg ccccatcggt cggtcctgtg
     2341 aggaaggaga aggacccgat gtgggtgggc cctcgtgtct cgagcagagg accccgaccg
     2401 tggcagactg tccccgtgag cgacgaggag ctcctcgcca aggcggaagc ggccctgact
     2461 gagatcaaga tgagccaggg actgtccgac caccacgcgt ccgtgctggc cgcgctgcgc
     2521 atccggctca ccggcagcgc gggcgagtcg ctcgacgagc tgctcgcggc ggcgggcgac
     2581 atcgggcccg acgaccgcct ccaggaggag atcgagaagg cccaggccgc gaaggcgcgc
     2641 aagccctcgc tcgacgacct gctcacgcag gagaagccga agcgggactg gccaggctga
     2701 tctccgtcag gcacggcggc gcaccggttc gagttctagg acttgcgtag ggtcacactc
     2761 cgagacgttc ggccgatgtc ccggtccccc agatgtccta cggtcgggga tgtgcagaca
     2821 accgattccc agccgatact gctgccccgc ccagccgacc cccgccgagt cctctgcacc
     2881 gcgtgcgagc acacggagtt cgtccacggc gacttcgagg atcgccgttg tctctactcc
     2941 gagtgcgcct gcggtggatt cacactggag gctgcccggc atgcctccga ggcgtcatag
     3001 ctccgcgcaa cgtcgaacac gaagaccgcg gatcctgcct cacgggacca ggccgaacac
     3061 ggtgctggcc gcgcccgacc cgttccggtt catcggtccg ccgatcacga tccagcctcc
     3121 ggtcgtgggc atcgcgccga ggttcgtgag gttctcgagc gtgaaccggt gccggttcag
     3181 cgtgaggtag gactcccagt agttcgcgtc ggccgatggg tcgggaccga acgtgtccgt
     3241 gccgagcgca ccgcgcttgg cgagcacgcc ggtctcgatc agccaccgca cggccccctt
     3301 cgagaagccc ggttggctgt acttcgcgcc cggctggccg cagttgtaga aggtcggctc
     3361 gtccttggcg agcgtcggac cccagaacgc gtcgcatccg gtccacagca gcaccgcggc
     3421 gcccgacggc atctcgccgt gcccggtctc ccagtcctgc aggtcggtct tcgtgacgac
     3481 gtggttcgca tcggcggcga cggcgtcgcg cacgtcgacg acgaccgccg gcaacacgta
     3541 gtcggccgcg tcgagatctt cggcgcacgg cttcccgtct cggaagtggc acggggcgct
     3601 gtagtgcgtg ccggtgtgct cgccctcacg cacgtactgc atgtagaagc catcgtcctc
     3661 gacagtcgcg accgtcgtga gcgtgaacgc ggggtcgccg gggaacagcg gcgtgcgcgc
     3721 cggtgcgttc acgtggctga ggctcacgac ctcgctgaag cccggcaacg tggtggagtc
     3781 gtcacccccc cgacgggcgc cagcttgtcc gacgacgtag ccacccgcga cgagcgcgac
     3841 ggcggcgagc agagcgacca acttcatgcg catgccagac ctcctcgatc gatgcgtgca
     3901 tcgtcgggca ggatgcgcgc ggggcgcaac tccgagtcga ccgcgcccga ctaggaagtc
     3961 ggcggtcact cgaccgtgcg accggtgtcg agaccgaagt cccagacatc gatcgacggc
     4021 gggtccactc cgaggttcgt cagggcggta cagacctgac tgcgatggtc ggtcccgtgg
     4081 tgcagcgcct gggcgagccg gatgccgatc ggcgcgtctc gctcgaagcc atcactctca
     4141 tcgacctcgt gcacgacggt gtccgggtca gggtggtcgc ccaggagccg gagccaggcc
     4201 tccccgtcgt cctgcatcgc ggccctgagg tgtcccaggt ccatctcgcc ctcctcgatg
     4261 caacgggcgg cgtcgccggt caggtcgaac aggtaccacg agtcggcccc gaccaggtgt
     4321 cgcatcgtgt cgaggatcga gccataggtt ccggggacgg cggccgcgag ccgttcgggc
     4381 gtgagcaccg tgcatgcgtc gatcaggcga accgtcgccc aggcgtgatg ggcgaacgca
     4441 tcctccagcg ggtatctgct catccgcgaa ccctaccccc cggttccacc acacccgaaa
     4501 gcagatcctc atgacccctg gtcggggccg agttgactgg gtttgccgtt ccgcgctaca
     4561 cctcgcttga acaacgcctt ccggcgagga ggggtcgaca tgcggccacg acggcacggc
     4621 caccaagttt gcctcatgct cgtggtcgcg ctggttgtcg gcgccaccct gggtgttgct
     4681 tcgagcgccg gcgcgaagat cgtcgggcag cgcgtcgccg ggaacctgcg ccaggcggtc
     4741 gccttcacgt tcgacgatcg tgatcggctc tgggtcgtcg agaagcaggt cggctcgatc
     4801 tatgtgcaga agctcgggag ccgacagcgc caccgtttct tccggatccc cgacgtctac
     4861 ggga
//
LOCUS       DATDBZ010000161         3576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1057025, whole genome shotgun sequence.
ACCESSION   DATDBZ010000161 DATDBZ010000000
VERSION     DATDBZ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057025"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VIX62_03915"
     CDS             <1..1316
                     /locus_tag="VIX62_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY5859408.1"
                     /translation="IPIIAVTGSNGKTTTTRMIAHIFRGMGRRVGMTTTDGIYIDERL
                     VKRVDASGPKSAQMVLQNPRVDTAVFEVARGGILREGLGYGRNDVGVVLNVTGDHLGL
                     REIETLDQLAAVKRVVIEAVPRNGWGVLNADDPLVLAMRNHCSGNVILFTMAEKHDLV
                     ERWIRRGRKAVVLERSDRGEMMVIKEGRRTMPIAWVHTLPSTFDGKARMMVQNAMAVA
                     AAAHAAGAHLHDIRQGLRSFTTSIYQAPGRLNVFDLDGVKVVLDYAHNAAALETLGAF
                     VERLVADSATGERPGEPSWSANLRVAVVATAGDRRDEDMKELGRIAARYFDEVIVRED
                     VKSRGRQRGETAELIREGVEDSIVQGGARAGSVEVVLDEMEAVRRALDRSRPGDLVVL
                     CVDYATEVYKEIEKRSGVASPDVLRASEGNLVDAVGGDPDLFVQG"
     gene            1375..1656
                     /locus_tag="VIX62_03920"
     CDS             1375..1656
                     /locus_tag="VIX62_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY5859409.1"
                     /translation="MAELTALTTVVRDPSATIGFGKRVGEGTSEAIGRIERVAQADAL
                     NAKLADVDRALAKIDEGTYGYCDRCVAAIPDERLVARPWAVLCLRCSAA"
     gene            1718..>3576
                     /locus_tag="VIX62_03925"
     CDS             1718..>3576
                     /locus_tag="VIX62_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5859410.1"
                     /translation="MGGFLYRVGRTSAHHRFVVIGVWIVLAVLAVGAVKSFGAKTDNS
                     LTLPGTNSQAAFDLLADQFPPQQNGTNPFVFHVDHGKLTDAANKAAITASYKAIKASA
                     QVYSVTNPVTKDAAKAGLLSQDGRTGLMPVLLKVESGFITERLAKKVLDATEPARKAG
                     IQVAVGGSIGSVLSTPDTKQSEILGNLAAMVILALVFGSLVAMGTPILTAVFALSIAT
                     SVIGLLGHVMAIPSVAPTLATMIGLGVGIDYALFLVTTHLEHLDEGVEVKESIARSVS
                     SSGSAIVFAGGTVVIALLSLVVAGIPLVSALGYAAAIAVFCAVLASITLLPAILSLLG
                     EWVRKLRIPRFLTPRKRPVGERRWDAWARGVARHPWVVVVVSLALLIPLIIPLFDLAL
                     GQEDIGVTPKDTTERQAYDLTTQGFGVGYNGPLLISSMLDPVATPSAEYTKKDAKAKA
                     LQKELEKKQKQLEAEADELKAQQATLERQQANLERRAAALQEQELVLEREGAQLRAEE
                     ASLRAQAERLVRRGAPIVAHLGFILGREAIVEDLIDSTTNPDRLRRLRARLARLKTKE
                     ATTRARLAPLVARAESLFAQAESLAAQAERLEAQAAALKTQGDELRSQGDELRA"
BASE COUNT          624 a         1125 c         1261 g          566 t
ORIGIN      
        1 ggatcccgat catcgcggtg accggttcca acgggaagac cacgacgact cggatgatcg
       61 cgcacatctt ccggggcatg gggcggcggg tgggcatgac caccaccgac gggatctaca
      121 tcgacgaacg attggtgaag cgggtcgatg cgagtggccc gaagtcggcg cagatggtcc
      181 tgcagaaccc acgggtcgat accgccgtgt tcgaggtcgc gcgcggaggg atcctgcgcg
      241 aaggactggg gtacggtcgc aacgatgtcg gcgtggtctt gaacgtgacc ggtgatcatc
      301 tggggcttcg ggagatcgaa accctggacc agctcgcggc ggtgaagcgc gtggtcatcg
      361 aggcggtgcc acggaacggc tggggagtcc tgaacgccga cgaccccctg gtcctggcga
      421 tgcggaacca ttgcagcggc aacgtgatcc tgttcacgat ggcggagaaa cacgatctgg
      481 tcgagcggtg gatccgtcgt ggacgcaagg ccgtggtcct cgaacgcagc gatcgcggcg
      541 agatgatggt catcaaggaa ggccgccgca cgatgcccat cgcgtgggtc cacacgctgc
      601 cctccacctt cgatggcaag gcgcgcatga tggtccagaa cgccatggcg gtcgcagccg
      661 ccgcgcatgc cgccggggct cacctgcacg acatccgcca aggcctgcgc tcattcacga
      721 cctcgatcta ccaagcgccg ggacgactca acgtgttcga tctggacggg gtcaaggtcg
      781 tgctggacta cgcgcacaat gcggcggcgc tcgagaccct gggggccttc gtggagcggc
      841 tggtcgcaga ctccgccacg ggggagcgcc caggagagcc ctcgtggtcg gcgaacctgc
      901 gggtcgcggt ggtcgccacg gcaggcgatc gtcgcgacga ggacatgaag gaactcggtc
      961 gcatcgccgc ccgctacttc gacgaggtga tcgtgcggga ggacgtcaag tcgcgaggtc
     1021 gccagcgagg tgagaccgcg gagttgatcc gcgaaggtgt cgaggactcg atcgtccagg
     1081 gtggtgcacg cgccggcagc gtcgaagtcg tgctcgatga gatggaggcc gtccgccgtg
     1141 cgctcgaccg ctctcgcccc ggtgatctcg tggtcctgtg cgtcgactac gccacggagg
     1201 tctacaaaga gatcgagaag cgcagcggtg tcgcgtcgcc agatgtgttg cgcgcttccg
     1261 aaggaaacct cgtggacgcg gtcgggggag accccgacct cttcgtccag gggtaggtcg
     1321 gccgtggata cctccgcgat cggatccgag cttcgcgtac gccacgaaga gttggtggca
     1381 gagctcaccg cgctgaccac cgtggtgcgc gacccgtcgg caacgatcgg gttcggcaaa
     1441 cgggtggggg aagggacgag cgaggcgatc gggcgcatcg aacgtgtcgc ccaggccgac
     1501 gcgttgaacg cgaagctcgc cgacgtcgac cgggcgctcg cgaagatcga cgagggcacg
     1561 tacgggtact gcgatcgatg cgtcgcggcg atccccgacg agcgcctcgt cgcacggcca
     1621 tgggccgtac tgtgcctgcg ctgcagcgcg gcctgaggcg ccgaggctcc tccctgggct
     1681 aggctcgcca ggcgcgtatc gagtgtggag gtcggtggtg ggagggttcc tgtatcgggt
     1741 gggccgcacg tcggctcacc accggttcgt cgtgatcggc gtgtggatcg tgctggccgt
     1801 gctggcggtc ggcgcggtga agagcttcgg ggcgaagacc gacaacagcc tcacccttcc
     1861 aggaaccaac agccaggcgg ccttcgacct gctcgccgat cagttccctc cccagcagaa
     1921 cggcacgaac ccgttcgtgt tccacgtcga tcacggcaag ctcaccgacg ctgcgaacaa
     1981 ggcagcgatc accgcctcgt acaaggcgat caaggcatct gcgcaggtat acagcgtcac
     2041 gaacccggtg acgaaggacg cggcgaaggc ggggctcctg tctcaggacg gacggacggg
     2101 gttgatgccc gtgctgctga aggtcgagtc cgggttcatc acggaacggc tcgcgaagaa
     2161 ggttctcgac gccaccgaac cggcgaggaa ggccggcatc caggttgcgg tcggcggatc
     2221 gatcggcagc gtgctgtcga cgcccgacac caagcagagc gagatcctgg gcaacctcgc
     2281 tgcgatggtc atcctggcgc tggtgttcgg gagcctggtc gcgatgggca cgccgatcct
     2341 cacggcggtc ttcgcgcttt cgatcgcgac ctcggtgatc gggctgttgg gccacgtcat
     2401 ggcgatcccc tcggtggcgc ccacgttggc gacgatgatc ggattggggg tggggatcga
     2461 ctacgccctg ttcctcgtga cgacgcacct ggagcacctc gacgaagggg tcgaggtgaa
     2521 ggagtcgatc gctcggtccg tctcgtcatc ggggagcgcg atcgtcttcg ctggaggcac
     2581 ggtcgtgatc gcgctgctgt ccttggtcgt ggcggggatc ccgctcgtca gcgcgctcgg
     2641 ctacgccgcc gcgatcgccg tgttctgcgc cgtcctcgcg tcgatcaccc tgctgccggc
     2701 gatcctgtcg ctactcggcg agtgggtgag gaagctccgg atcccccggt tcctcacgcc
     2761 ccgcaagcga cctgtgggtg aacgccggtg ggatgcgtgg gcgcgagggg tggcgaggca
     2821 cccctgggtc gtggtcgtgg tgtccctcgc cctcttgatc ccgttgatca tccccttgtt
     2881 cgacctcgcg ctcggccaag aagacatcgg tgtcacgccg aaggacacca ccgaacgcca
     2941 ggcatacgac ctgacgaccc aagggttcgg cgtcggctac aacggccccc tgttgatctc
     3001 gagcatgctc gatccggtcg cgacgcccag cgccgagtac acgaagaagg acgccaaagc
     3061 caaagctttg cagaaggaac tggagaagaa acagaaacag ctcgaggcgg aggcggatga
     3121 gctcaaggcg cagcaggcga ccctcgaacg tcagcaagcc aacctcgaac gccgcgctgc
     3181 cgcgctccaa gagcaggagt tggtgctgga acgagagggc gcccaactgc gtgctgagga
     3241 agcgagcctg cgggcgcaag ccgaacggtt ggtgcggcgg ggggcaccca tcgtcgccca
     3301 ccttgggttc atcctgggtc gtgaagcgat cgtcgaggat ctgatcgaca gcacgaccaa
     3361 cccggaccgt ctgcgccggc tgcgagccag gctcgcccgg ctcaagacca aggaagccac
     3421 cacgcgtgcc cggctcgcgc ccttggtggc tcgtgccgag tcattgttcg cacaggccga
     3481 gtccctcgcc gcccaggcgg agcgactcga agcccaggct gccgcactca agacccaggg
     3541 tgacgaactc agatcgcaag gcgatgagct ccgcgc
//
LOCUS       DATDBZ010000162         6002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1280575, whole genome shotgun sequence.
ACCESSION   DATDBZ010000162 DATDBZ010000000
VERSION     DATDBZ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280575"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VIX62_03930"
     CDS             complement(<1..366)
                     /locus_tag="VIX62_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859411.1"
                     /translation="MTEPHPTTEAQHRNMFVLRALVVLGFAFLLLAVAPHAAHALDLG
                     DPVGTVTDTIDPVTDTLEPVTDTLDPVTQPVADTVEPVTQPVTDTLEPVTDTVGQVVE
                     PVTDTLEPVTDTVTDTLDPV"
     gene            complement(523..774)
                     /locus_tag="VIX62_03935"
     CDS             complement(523..774)
                     /locus_tag="VIX62_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859412.1"
                     /translation="MQHTSDRWADVITLGNARFGQLHGGPFDGRCFPLGAGTPEVLHV
                     PVEGSPPADGQLRRRTRTLRYELRDGRYRFVAARSAAAA"
     gene            992..1777
                     /gene="panB"
                     /locus_tag="VIX62_03940"
     CDS             992..1777
                     /gene="panB"
                     /locus_tag="VIX62_03940"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY5859413.1"
                     /translation="MNVSIHDLRAWKTEGRRFVMLTAYDAPTAQILDAAGVPVLLVGD
                     SLGRNVLGYDNELPVTMDDMLHHTKAVARGTEHAMVVGDLPFMSYQASLEDGVRNAGR
                     LIKEGGAHAVKLEGPQLDLVHRLVEIGIPVMAHVGLTPQSVNGLGGYRVQGRGEAAHK
                     VLEQATQLEKAGAFSIVLEAMPSDLGAEISAALQIPTIGIGAGPHTDAQVLVINDLLG
                     ITENPPKLAKAYVDLRATITDAVTAFTRDVESGTFPDEDHSYS"
     gene            1825..3321
                     /locus_tag="VIX62_03945"
     CDS             1825..3321
                     /locus_tag="VIX62_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5859414.1"
                     /translation="MDTWPRRIAWIAGPIAVVILGGLLGVDATRWQVSATGWLAGALA
                     LVGVVHHRTSRDPDALLLGIAGGVLLLQDMFSSFVVPHVGLGEADLDLLGSSLTAVVT
                     GWAVAAGCIVLVSPWWDRRGRPRIAASSFGLILLAIIVVADILLLVVPLSPSNEINPF
                     FGSVVPFTVLTWIWLVLGTIGWCLVAVRQCFLVPRGNQHGALAAGAIVATAFLLSFAA
                     IASGATWAGEFAIGVTFWTPPIAIGLLLLAASLMQRSDASRMRRETDRAEEIVEGRAE
                     IASMVAHEVRGPVTTIRGIASTAATHYDGLDDAERREFFELIEQESRRLLAAVDQTSL
                     ALRVDARSLTYTMGLTDLAMAVRQGVEKAAVTNHPVHVVAEEGVELMGDRARIAQLVT
                     QLIDNASKFSPPGSPITVRSVRDGDDALIEVDDRGPGIPVEQREVVFDRFVRWRPKGY
                     EEQPGNGLGLFISRGLAAEHQGEISVEDGPGGGTMLRVRLPVGGAARG"
     gene            3314..3988
                     /locus_tag="VIX62_03950"
     CDS             3314..3988
                     /locus_tag="VIX62_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5859415.1"
                     /translation="MGDSDQKVTLLICDDHKILTDALATVVGLDPTLVMVTPPVHDPE
                     TAIEVCTEQLPDVVLMDIVFKGGMSGIEATRKIKEVSPSTKVVIMTAHDEDRLLVEAV
                     EAGASGFLGKDEAAEELLSAAKSAADGEVLIDPATLTRLLAQVAREREAQRDAQNLLD
                     DLTEREREILQLLAEGMRNDDIAAKLFISPQTVQTHVRNILGKLRVHSKLEAVAFAVK
                     HGAITV"
     gene            complement(4177..5364)
                     /locus_tag="VIX62_03955"
     CDS             complement(4177..5364)
                     /locus_tag="VIX62_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY5859416.1"
                     /translation="MEATFGLAAGELLAVELPPGPAWVEVVHRCWHGGVPFLPLDVRL
                     GEDERRRLIDRAQPAALLDATGELTFFAPGEPVTEGVAVVVASSGTSGDPRLVELPRS
                     AIEASVAGSTASLGVGRDDPWISCLTPAHIGGLLVLLRAEILEVPAVVLDGFDAGAVE
                     AARHRGAHVSLVPTLLGRLLDHGGGQAPGGLLLVGGGALELAFRTRAERGGARVVQTY
                     GLTESCGGVVYDGVPFDGTSVRVGDQDRIELRGPTIMEGYRNDAAATGEAFDVQGWLR
                     TGDAGSIDDLGRVSVHGRLDDAIRTGAETVWPDEVERALTHHPKVRDVAVAGRPHPGW
                     GQQVVAFVVPASIDAPPEVEEVRSFLADRLASFKAPRELILVPEIPRTASGKVQRSAL
                     PFT"
     gene            complement(5405..>6002)
                     /locus_tag="VIX62_03960"
     CDS             complement(5405..>6002)
                     /locus_tag="VIX62_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859417.1"
                     /translation="GTPPFMVSPDPLLVEQGQDLTVTYHAPGNAGEKVVIVPMGGDPA
                     TDGIDQAGTPAADDVDGSVVFDTATWISGGYDAVLVDASDTELSRNLFWLRLPGERPV
                     LRSEQWRYDVGEPIRISWEGAPAHRFDWIGIYARDADPQVAYYKDYLYTRGTLVGSAT
                     FRGGTVKHWPLRPGRYTAYLLVSDIYLDVAGVDFVIER"
BASE COUNT          983 a         2056 c         2057 g          906 t
ORIGIN      
        1 gaccggatcg agggtgtcgg tcacggtgtc agtcacaggc tcgagggtgt cggtcaccgg
       61 ctcgaccacc tggccgacgg tgtcggtcac cggctcgagg gtgtccgtca cgggctgggt
      121 caccggttcg acggtgtcag ccacgggctg ggtgaccgga tcaagggtgt cggtcacggg
      181 ctcgagggtg tcggtcaccg gatcgatcgt gtcggttacg gtcccgaccg ggtcaccgag
      241 atcgagcgca tgagccgcat ggggggccac ggccaggagc aggaacgcga acccgaggac
      301 cacgagcgcg cgcagcacga acatgttgcg gtgctgggct tctgtcgtgg gatggggttc
      361 ggtcatctgg acgcgtctcc ggtacatctc cggcggacga ggcgagaagc ctcgtcacgt
      421 gtccttccta taaccccttc gcgggccggt tgaaaccgga tcaccgctct cacgacccta
      481 ccccaggatc tcgaacctcg cacccccgag agggaagaaa tctcaggccg cggctgctga
      541 gcgcgcggcc acgaatcggt acctgccgtc ccgaagttcg tatcgcaacg ttcgggtccg
      601 gcgacggagc tggccgtctg ccggtggcga accttcgacg ggaacgtgca ggacctcggg
      661 tgttcccgct ccgaggggga agcaccggcc gtcgaacggc cctccatgga gttggccgaa
      721 gcgcgcgttg ccgagggtga tcacgtccgc ccagcgatcc gaggtgtgct gcatcacgat
      781 ctccttcgtt cgatggtgga acggtatcca aggggtctga caccgttctc gaacgaacgt
      841 tcgataccca gggccttccg gttccatgca tctgggttgg ctgccgaaac atccatggaa
      901 cggttgcccg ggcacccggg aaggctgcgg gatccgcgca cgttagactc agcggaacac
      961 gaaggccgtg cacttgaagg aggcgcgagc gatgaacgtg tcgatccacg acctgcgagc
     1021 gtggaaaacc gaagggcgtc ggttcgtgat gctgaccgcc tacgacgccc cgaccgccca
     1081 gatcctcgac gccgccggcg tgccggtgct gctggtcggg gactcgctcg gtcgcaacgt
     1141 cctgggctac gacaacgaac tccccgtgac gatggacgac atgctccatc acacgaaggc
     1201 ggtggcccgg ggcacggagc acgccatggt cgtgggcgac ctgccgttca tgagctacca
     1261 ggcctccctg gaggacgggg tgcgcaacgc cggccggttg atcaaggagg gcggagcgca
     1321 cgcggtcaag ctcgaagggc cgcagctcga cctcgtgcac cggctggtcg agatcggcat
     1381 cccggtgatg gcgcacgtgg gcctcacgcc gcagtcggtc aacggcctgg gcggctaccg
     1441 ggtgcaaggt cgcggcgagg ccgcacacaa ggtgttggaa caggcgaccc aactggagaa
     1501 ggcgggcgcg ttctcgatcg tcctggaggc gatgcccagc gatctcggcg ccgagatcag
     1561 cgccgcgctg cagatcccca cgatcgggat cggcgccggc ccgcacaccg acgcccaggt
     1621 gctggtgatc aacgacctgc tcggcatcac cgagaatcca ccgaagctcg cgaaggccta
     1681 cgtcgatctc cgagccacga tcaccgacgc cgtgacggcg ttcacccgag acgtcgagtc
     1741 gggtacgttc ccggacgagg accacagcta ctcgtaggcg acgcctcgta ggaccgcccg
     1801 gccaccggtc ctagactcgc gcgcatggac acctggccac gacgcatcgc gtggatcgcc
     1861 gggccgatcg ccgtggtgat cctgggagga ctgctcggcg tcgacgccac ccgatggcag
     1921 gtttccgcga ccggttggct agcgggggca ctcgcgctcg tcggcgtcgt ccaccatcga
     1981 acgtcgaggg acccggacgc cctcctgctg ggcatcgcag gaggggtgct gctcctgcag
     2041 gacatgttct ccagtttcgt ggtgccccac gtcggtctgg gcgaggccga cctggacctg
     2101 ctcggttcct ccctcaccgc ggtggttacc gggtgggcgg tggccgcggg atgcatcgtg
     2161 ttggtgtccc cttggtggga tcgtcgtggc cgtccgagga tcgcggcatc gagcttcgga
     2221 ctgatcctgc tggcgatcat cgtggtggcc gacatcctcc tgctcgtcgt ccccctctct
     2281 ccgagcaacg agatcaaccc cttcttcggc tccgtggtgc ccttcacggt cctcacgtgg
     2341 atctggctcg tcctcggcac gatcgggtgg tgcctcgtcg cggtgcgcca gtgcttcctg
     2401 gttccacggg ggaaccagca cggagcgctc gctgctggcg ccatcgtggc gaccgcattc
     2461 ctgctctcgt tcgccgcgat cgcctccggc gccacgtggg cgggcgagtt cgcgatcggc
     2521 gtgaccttct ggacaccgcc catcgccatc ggactgctgc tgctcgcggc ctcgttgatg
     2581 cagcgttccg acgcatcacg tatgcggcgg gaaaccgacc gcgccgagga gatcgtggaa
     2641 ggcagggccg agatcgcgag catggtggcc cacgaggtgc gaggcccggt gaccacgatc
     2701 cgagggatcg cctcgaccgc cgccacgcac tatgacgggc tcgacgacgc cgagcgccgg
     2761 gagttcttcg agctgatcga gcaggagtca cgacgcctat tggccgccgt ggatcagacg
     2821 tcgctggcgc tgagggtcga cgcgcgctcg ttgacgtaca cgatggggct gaccgatctg
     2881 gccatggcgg tgcgccaggg ggtggagaag gctgccgtca ccaaccaccc ggtgcacgtg
     2941 gtcgccgaag aaggcgtgga gctcatgggg gaccgcgcca ggatcgccca gttggtgacg
     3001 cagctgatcg acaacgcatc caagttctcg ccgcccgggt ctcccatcac ggtgagatcg
     3061 gtgcgcgacg gcgacgacgc cctgatcgag gtcgacgacc gcggtcccgg tatcccggtc
     3121 gagcaacgcg aggtggtctt cgatcggttc gtccgatggc ggccgaaggg ctacgaagag
     3181 caaccaggaa atggtctcgg gctgttcatc tcccggggtc tggctgccga acaccaaggg
     3241 gagatatccg tcgaggacgg cccgggcggg ggtacgatgc tccgcgtccg gctgcccgtg
     3301 ggaggggctg cacgtgggtg attccgacca gaaggtcacg ttgctgatct gcgatgacca
     3361 caagatcctg accgacgcgt tggccaccgt cgtcggcctc gaccccaccc tcgtcatggt
     3421 gacgccgccg gttcacgacc cggagacggc catcgaggtc tgcaccgagc aactccccga
     3481 cgtggtcctg atggacatcg tgttcaaggg cggcatgagc gggatcgagg ccacccgcaa
     3541 gatcaaggag gtctcaccct ccaccaaggt cgtgatcatg acggcccacg acgaggaccg
     3601 gctcctggtc gaagcggtcg aagcgggagc ctccgggttc ctgggcaagg acgaggcggc
     3661 cgaggagctg ctgtccgccg cgaagtcggc cgccgacggc gaggtgctga tcgaccccgc
     3721 caccctcacg cgcctcctcg cccaggtcgc gcgcgaacgc gaggcgcagc gcgacgcaca
     3781 gaacctcctg gacgatctca cggagcgcga acgcgagatc ctgcagctgc tcgccgaagg
     3841 catgcgcaac gacgacatcg ccgccaagct cttcatcagc ccgcagaccg ttcagaccca
     3901 cgtccggaac atcctgggca aactgcgtgt ccactcgaag ctcgaagccg tcgcgttcgc
     3961 cgtcaagcac ggcgccatca cggtctagcg ctcccagctg cccgggctac ccggtccggg
     4021 gtaggcagga ttacctctcg cacggaatgc agccttgcgt ccccgcgcgc cgatgaagtg
     4081 ggcagacaac gcgggacgcc accccctcgg cggcccgcca catgacgaag gacccgagtc
     4141 cacccctccc ggactcgggt cctttctcat gtggggtcag gtgaagggaa gcgccgagcg
     4201 ctgaaccttc ccggaggcgg tccgggggat ctcgggcacg aggatcagct cgcgaggtgc
     4261 cttgaacgac gcgaggcggt cggccaggaa cgagcgcacc tcctccacct ccggcggtgc
     4321 gtcgatcgaa gcggggacga cgaacgcgac gacctgctga ccccatcccg gatgcggacg
     4381 gccggcgacc gccacgtcgc ggaccttcgg atggtgggtc agcgcgcgct cgacctcgtc
     4441 gggccagacc gtttcggcgc cggtgcggat cgcatcgtcg agccggccgt gcacgctgac
     4501 ccggcccaga tcgtcgatcg agcccgcgtc gccggtgcgc agccagccct ggacgtcgaa
     4561 ggcctcgccg gtggcggcgg catcgttgcg gtagccctcc atgatcgtgg gcccacggag
     4621 ctcgatccga tcctggtcgc cgacccgaac gctcgtgccg tcgaaaggca cgccgtcgta
     4681 gaccacgccg ccgcaggact cggtgagacc gtaggtctgc accactcgcg caccgccgcg
     4741 ttcggcgcgg gtccggaacg ccagctcgag cgctccaccg cccaccagga gcagccctcc
     4801 cggcgcctgg ccacctccgt ggtccaggag tcgtccgagc aacgtgggga ccagcgagac
     4861 atgtgcgccc cgatgacgtg cggcctcgac cgcaccggcg tcgaacccgt ccaggacgac
     4921 ggctggcacc tcgaggatct cggcacgcag cagcacgagc aacccgccga tatgggccgg
     4981 cgtcaggcat gagatccagg gatcgtcacg gccgacaccc aacgacgccg tggaacccgc
     5041 caccgacgcc tcgatcgccg agcgagggag ctcgacgagg cgaggatccc cggacgtgcc
     5101 cgaggaggcg acgaccacgg cgacgccctc ggtcacgggt tcgccggggg cgaagaacgt
     5161 gagttccccg gtggcatcga ggagggcggc aggctgcgct cgatcgatca accgccggcg
     5221 ctcgtcctca cccagtcgaa cgtccaacgg caggaacggc acgccgccgt gccaacatcg
     5281 gtgcacgacc tcgacccacg ccggaccggg tgggagctcg accgcgagca gttcgcccgc
     5341 ggccagaccg aacgtcgcct ccatgcgccg agcgtacccg cgagggcacc gcccctgggc
     5401 ccgctcagcg ctcgatgacg aagtcgacgc cggccacgtc gaggtagatg tcgctcacca
     5461 gcagataggc ggtgtatcgc cccggtcgca gcggccagtg cttcacggtg ccgccgcgga
     5521 acgtggcgct gcccacgagc gtgccccgcg tgtagaggta gtccttgtag taggccacct
     5581 gaggatcggc atcgcgagcg tagatcccga tccagtcgaa ccggtgcgcg ggcgcccctt
     5641 cccagctgat gcggatcggc tcccccacgt cgtagcgcca ctgctccgaa cgcagcaccg
     5701 ggcgttcgcc cggcaggcgg agccagaaca ggttccggga aagctccgtg tcgctcgcgt
     5761 cgaccagcac ggcgtcgtag ccgccggaga tccaggtcgc ggtgtcgaac accacgctgc
     5821 cgtcgacgtc gtcggcggcg ggggttcccg cttggtcgat cccgtcggtc gcggggtcgc
     5881 cgcccatcgg cacgatcacg accttctccc cggcgttgcc cggagcgtgg tacgtgaccg
     5941 tcagatcctg cccctgctcc accaggagtg gatcgggcga caccatgaac ggcggcgtgc
     6001 cc
//
LOCUS       DATDBZ010000163         6565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1897109, whole genome shotgun sequence.
ACCESSION   DATDBZ010000163 DATDBZ010000000
VERSION     DATDBZ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897109"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VIX62_03965"
     CDS             complement(<1..321)
                     /locus_tag="VIX62_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859418.1"
                     /translation="MITAFLALVATLWTGATVEALTRTAAAPDSVVMVAGRSFVTSSS
                     IRLWLAAGAGAVLVALWCLTVWSFARRRARRPSAADNADQAPPIPSCLPASARCSCAW
                     RSCRN"
     gene            complement(339..770)
                     /locus_tag="VIX62_03970"
     CDS             complement(339..770)
                     /locus_tag="VIX62_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859419.1"
                     /translation="MVGLVLVVVASGLSILTLEAVAESARSGARDLVVFGHEIASPGS
                     PGGTWILIGLAVAAALAWSAAIAWSRGHRLERRMAAELDGRRDLPPRREATRSGHGSV
                     TASRPLGLRTAPPPERPGNDQPTRRDELAARRARRAIHPAN"
     gene            931..1392
                     /locus_tag="VIX62_03975"
     CDS             931..1392
                     /locus_tag="VIX62_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859420.1"
                     /translation="MKRSSPLRAAAVVLGCLLASAASACTPDDPPEATSVPPGSSAPA
                     PRSDAYVYADPAGITAELTLDTSGATLTIENATGRALARPGVYVLDARDGRRVAWTVV
                     AAAPIPDGATAEFAVERPASPEDKHIGLAALLFGGEDHGAFVPPHPGQEAA"
     gene            1392..3059
                     /locus_tag="VIX62_03980"
     CDS             1392..3059
                     /locus_tag="VIX62_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5859421.1"
                     /translation="MRRTRVLGAVVVLSITVGLTPAGSQEADLEERLRAEACRVPLEL
                     LVRAAHGHRADRSGDIDILPAEPDFVGHGGLPHSGPWDYVGKVPMLWYGPGYIRAQGS
                     VPRTVTLADIAPTQGALVRYPFAAPDGEVMHEALVPEAERPDEPPRLVVVVVWDGAGR
                     NVLSTWPNAWPELTKLIEGGTWYEHAEVGSSPPSTAQIHATIGTGAFSKHHGLVAHQF
                     RIGNRLATPWEAGPAYLMLPTFADLYDRANGNRPLVGTLATVAIQLGMMGHGSFWGGG
                     DQDIAVLRELEGAATLGAEGLSWNLTKPVQPYYRVPSYVNDLPPITEYYDEVDLLDGR
                     ADGMWHELHLSEANVEEALRTPARIPWQTRLVQEVIDREGFGADDTPDLLFVNYKLID
                     EVGHQDSMNSVQMRDSVRSQDADLPELVDMLNDQVGEGRWVMALTADHGHTPDPTVTD
                     ATVISPILVADAIQARFDHDGDDVRIVEFTQPTHVFIDRDELREQGATLEEVAAYLLT
                     LTKGQLQSPDFPAPPGVEDEPAFLAAYPSAMLEDLPCVQDALADEAA"
     gene            complement(3064..5343)
                     /locus_tag="VIX62_03985"
     CDS             complement(3064..5343)
                     /locus_tag="VIX62_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5859422.1"
                     /translation="MDPRAFLDELTADTDIASSLVHVQHLPARAPAIEPFPDGLPDLL
                     VSRLGLTGVRGLYPHQADGLAVLRDGRDLVIATGTASGKTLVYNAAFAEAALTRPKAT
                     ALYLYPTKALARDQLRAVRALKLPQVKASVYDGDTPRAERPLIRKNANLVMTNPDMLH
                     LSLLPDHARWADFLFRLSMVVVDEAHVCRGVFGSHVAMVLRRLRRLIAHYGGTPRWCL
                     ASATVGNPGELATRLTGMTFDEVTDDRSPRGEKLFAFWNPPVVDEDTGQRRSALSEAS
                     WLMGRLADRDVRTIGFTRSRRAAELLAEFARRDVAEAKTRARIKSYRAGYLAEDRRRI
                     ERELADGDLIAIASTSALELGIDIGTLDAAVLTGYPGTRASMWQQAGRAGRRDGDALA
                     ILVAQDDPLDQYLVNHPDDIFERPPEAAVIDPTNPYVLEPHLRCAAREVPLREEELGF
                     FGDVPDVKKAVERMTDRGELVQRREAWHDRGKDAPHRAVDVRAGGGHVYSIVIAGTGE
                     LLGTADEHRAYATLHPGAVYLHMGEQYLVEELDLVQRVAVVASADPDYYTQARDVTDI
                     EVADVLSRTSIGEVEQSFGSVRVTNQVTSFVRKLVATGEVLDEVPLPLPPVELETRAV
                     WWTIPVSVLDRAAVGPRDVPGAIHAAEHAAIGLLPLVATCDRWDIGGVSTPMHPDTGM
                     TTIFIYDGYPGGAGITERGARDADRWLRATLEAIRQCPCSRGCPSCVQSPKCGNGNEP
                     LDKTGAVSLLAAILGERWG"
     gene            complement(5354..>6565)
                     /locus_tag="VIX62_03990"
     CDS             complement(5354..>6565)
                     /locus_tag="VIX62_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEY5859423.1"
                     /translation="AIVAAAFILTWVAEAAERDVPRTLALAGLALIAVLPEYAVDFVF
                     ARKGGESFAETGAACLPLDGGTESPCSLALANMTGANRLLIGIGWTMVVFVAFWRLRA
                     KGRIVKGQHWGGVKLDREHSIEIAFLAIATIYSLTLPLKHSVTLFDSVILVAIFVLYT
                     VRVSRAPAEEPHLVGPARYLGTFSTAARRTTVITIAVFAAGLILASAEHFAEALVASG
                     ETLGVSEFLLVQWIAPLASEAPELLVAGLYAWRLNTSAGLGTLVSSKVNQWTLLVGTL
                     PIVFAVAAGGFHGLPLDTVQREELFLTAAQSFFAVAVISSLSMSLREAGILFGLFWFQ
                     FLLGGIVPESLHGVERIAVGILYLILGVGVLVRERGQLRGLFKDGLTASYDELSAERA
                     ADAAGDAATPG"
BASE COUNT         1005 a         2339 c         2311 g          910 t
ORIGIN      
        1 gttcctgcaa ctcctccacg cgcagctgca tcgagccgac gcgggcaagc aggaggggat
       61 cggcggtgcc tgatcggcgt tgtcggccgc cgaagggcga cgggctcggc ggcgcgcgaa
      121 cgaccacacg gtgaggcacc agagcgcgac gaggacggcc ccagcgccgg ccgccagcca
      181 cagccggatc gagctcgagg tgacgaagga gcgaccggcc accatcacga ccgagtcggg
      241 ggccgcggcc gttcgcgtga gggcctcgac ggtcgcgccg gtccacaggg tcgccacgag
      301 ggcgaggaag gcggtgatca cgggtaggtc ctcctggatc agttcgccgg gtggatggcg
      361 cgccgggcgc ggcgagcggc gagctcgtcg cggcgtgtgg gctggtcgtt gcctggtcgt
      421 tcaggcgggg gagcggttcg gagcccgagc ggacgcgacg cggtgacgga gccgtgcccc
      481 gatcgtgtcg cttcacggcg agggggtagg tctcgccggc cgtcgagctc cgcggccatc
      541 cggcgctcca gccgatggcc gcgcgaccat gcgatggccg cggaccaggc gagcgctgcg
      601 gcgaccgcga ggccgatcag gatccacgtg ccccccgggc ttcccggtga ggcgatctcg
      661 tggccgaaca cgacgaggtc gcgggcaccc gatcgggcgc tctcagcgac ggcctcgagg
      721 gtgaggatgg acaaccccga cgccaccacg accaacacca gacccaccat cggtcgttcg
      781 gctccttctg gggggaagga ccgttctcgg ttcggagacg gacgctacgc ccgggtgcgc
      841 ttcggggcga tacggcagat gcgggaggcc ggttgtccct cgtccgggcc ggccctcgcg
      901 ggggtccggc tggtagattc cgccgaaccc gtgaagcgtt cctccccact ccgagccgcc
      961 gccgtggtcc tcggctgcct cctcgcctcc gccgcgtcgg cctgcacgcc ggacgatcca
     1021 cccgaggcca cgtccgttcc cccgggtagc tcggcgccgg ccccccgctc cgacgcctac
     1081 gtctacgccg atccggcggg gatcaccgcc gagctgacgc tggacacgag cggcgccacc
     1141 ctgaccatcg agaacgccac cggtcgcgcc ctggcgcgac ccggcgtcta cgtcctggat
     1201 gcgcgcgacg gtcgccgcgt ggcctggacg gtggtggccg ccgcgccgat cccggacggg
     1261 gccaccgcgg agttcgcggt cgaacgtccg gcatcgcccg aagacaagca catcgggttg
     1321 gcggccctgc tgttcggagg cgaggaccac ggagcgttcg tgccacccca tcctgggcag
     1381 gaggccgcgt gatgcgacgg acccgcgtcc tgggcgccgt cgtggtgctg tcgatcaccg
     1441 tggggctcac gcccgcgggg tcgcaggaag ccgatctcga ggaacggttg cgcgccgagg
     1501 cgtgccgagt gccgctcgag ctcctggtgc gcgcggcgca cggccatcgc gccgaccgct
     1561 cgggcgacat cgacatcctc cccgccgagc ccgacttcgt gggccacggt ggattgcccc
     1621 actccggtcc gtgggactac gtcgggaagg tgccgatgct ctggtacggc cctgggtaca
     1681 tccgcgcaca ggggtcggtg ccgcgaaccg tgacgctcgc cgacatcgca ccgacgcaag
     1741 gcgcgttggt gcggtatccg ttcgccgcac ccgacggcga ggtgatgcac gaggcgttgg
     1801 tccccgaggc cgagcgtccc gacgagccgc cacgcctggt ggtggtggtg gtctgggacg
     1861 gcgcgggccg caacgtcctg tccacatggc cgaacgcgtg gccggaactg accaagctga
     1921 tcgagggcgg cacctggtac gagcacgccg aggtgggctc gtccccgccg agcaccgccc
     1981 agatccacgc cacgatcggc acgggcgcct tctcgaagca tcacggactg gtggcccacc
     2041 agttccggat cgggaaccgg ctcgccaccc cgtgggaagc cggtcccgcc tatctgatgc
     2101 tgccgacgtt cgccgacctc tacgaccgcg ccaacggcaa ccgcccgttg gtggggacac
     2161 tcgcgacggt ggcgatccag ctgggcatga tgggccacgg ttcgttctgg ggcgggggcg
     2221 atcaggacat cgccgtcctg cgcgagctcg aaggcgccgc cacgctcggc gccgagggcc
     2281 tcagctggaa cctcacgaag ccggtccagc cctactaccg ggttccctcc tacgtgaacg
     2341 acctgccgcc gatcaccgag tactacgacg aggtcgatct gctcgacggc cgcgccgacg
     2401 gcatgtggca cgagctgcac ctcagtgagg ccaacgtcga ggaagcgctg cgcacgccgg
     2461 cccggatccc gtggcagacc cgcctggtgc aagaggtcat cgatcgtgag ggcttcggcg
     2521 ccgacgacac gcccgacctg ttgttcgtga actacaagct gatcgatgag gtgggccacc
     2581 aggattcgat gaacagcgtc cagatgcgcg actcggtgcg atcccaggac gccgacctgc
     2641 cggagctcgt cgacatgctg aacgaccagg tgggcgaggg ccgctgggtc atggcgctca
     2701 ccgccgacca cgggcacacc cccgatccga cggtgacgga cgccacggtg atctccccga
     2761 tcctggtggc cgacgcgatc caggcccggt tcgaccacga cggcgacgac gtccggatcg
     2821 tcgagttcac ccagcccacc cacgtgttca tcgatcgtga cgagctccgc gaacagggcg
     2881 ccaccctcga ggaggtggcg gcctacctgc tgacgctcac gaagggacag ctgcagagcc
     2941 ccgacttccc cgcgcccccc ggcgtcgagg acgaaccggc gttcctcgcg gcctatccgt
     3001 cggcgatgct cgaggatctg ccgtgcgtgc aggacgcgct ggccgacgag gccgcctgaa
     3061 cggtcagccc catcgctcgc cgaggatcgc ggcgagcagc gacaccgccc cggtcttgtc
     3121 gagcggctcg ttgccgttgc cgcacttcgg cgactgcacg cacgagggac atccccgcga
     3181 acacggacac tgacggatcg cctcgagcgt ggcgcgcagc catcggtcgg catcgcgggc
     3241 cccgcgctcg gtgatccccg cgccgcccgg gtacccgtcg tagatgaaga tcgtggtcat
     3301 gccggtgtcg gggtgcatcg gcgtggagac gccgccgatg tcccaccgat cgcaggtcgc
     3361 caccaacggc agcaggccga tcgcggcgtg ttcggcggcg tggatcgcac ccgggacatc
     3421 ccgaggtccc acggcggcgc ggtcgagcac gctcaccggg atcgtccacc acacggcgcg
     3481 ggtctcgagt tcgacgggcg gcagcggcag cggcacctcg tcgagcacct cgcccgtggc
     3541 cacgagcttg cgcacgaagc tggtcacctg gttcgtgacg cgcaccgagc cgaacgactg
     3601 ttccacctcg ccgatgctgg ttcgcgagag cacgtcggcc acctcgatgt cggtgacgtc
     3661 gcgggcctgc gtgtagtagt cggggtcggc gctcgcgacg accgcgaccc gttgcaccag
     3721 atcgagttcc tccaccaggt actgctcgcc catgtggagg tagacggcgc ccgggtgcag
     3781 tgtggcgtag gcgcggtgtt cgtcggcggt gcccaacagc tcgcccgtgc cggcgatcac
     3841 gatggagtac acgtggccgc cgcccgcccg cacgtcgacc gcgcggtggg gagcgtcctt
     3901 gccgcggtcg tgccaggcct ccctgcgctg gaccagctct ccacgatcgg tcatgcgctc
     3961 gacggccttc ttcacgtcgg gtacgtcgcc gaagaacccg agctcctcct cacggagcgg
     4021 cacctcgcga gcggcgcagc gaaggtgcgg ttccagcacg taggggttcg tggggtcgat
     4081 caccgccgcc tccggtggcc gctcgaagat atcgtcaggg tgattgacca agtactggtc
     4141 gagcgggtcg tcctgggcga ccaggatcgc caaggcgtcg ccgtcccgtc gcccggcgcg
     4201 ccccgcctgc tgccacatcg aggcgcgcgt tcccgggtag ccggtgagca ccgcggcgtc
     4261 gagcgtgccg atgtcgatgc cgagttccag cgcgctcgtc gaggcgatgg cgatcaggtc
     4321 gccgtcggcg agttcgcgtt cgatccggcg ccggtcctcg gcgaggtagc ccgcccggta
     4381 gctcttgatg cgcgcgcggg tcttcgcctc ggccacgtcc cgcctcgcga actcggccag
     4441 gagctcggcg gcgcgacgcg accgcgtgaa cccgatcgtg cgcacgtcgc ggtcggcgag
     4501 acggcccatg agccacgacg cctcgctcaa cgcgctgcgc cgttgccccg tgtcttcgtc
     4561 cacgacgggc gggttccaga acgcgaagag cttctcgccg cgtggcgacc gatcgtcggt
     4621 gacctcgtcg aaggtcatgc ccgtgagccg ggtcgcgagt tcgcccgggt tgcccacggt
     4681 ggccgaggcc aggcaccacc tgggggtgcc gccgtagtgg gcgatcagcc gccggagccg
     4741 ccgcagcacc atcgcgacgt gcgagccgaa cacgccgcga cacacgtgcg cttcgtccac
     4801 caccaccatg gagaggcgga agaggaagtc ggcccaccgc gcgtggtcgg gcagcagcga
     4861 caggtggagc atgtccgggt tcgtcatcac caggttcgcg ttcttgcgga tcaacggacg
     4921 ctcggcacgg ggggtgtcgc cgtcgtagac cgaggccttc acctggggga gcttcagcgc
     4981 ccggacggcc cggagctgat cgcgggccag cgccttcgtg gggtagaggt agagcgcggt
     5041 ggccttcggt cgggtgaggg cggcctcggc gaacgccgcg ttgtacacga gcgtcttgcc
     5101 gctggcggtg cccgtggcga tcaccagatc gcgcccgtcg cgcagcaccg ccagcccatc
     5161 ggcctgatgc gggtacaggc cgcggacgcc cgtcaggccc aaccgcgaga ccagcaggtc
     5221 gggcaggccg tcggggaagg gttcgatcgc cggcgcgcga gccggcaggt gttgcacgtg
     5281 gaccagtgag ctggcgatgt cggtgtcggc ggtgagctcg tcgaggaacg cgcgggggtc
     5341 cacgcggcca aggctacccg ggtgtcgccg cgtccccggc ggcatcggcc gcgcgttcgg
     5401 cagagagttc gtcgtaggag gcggtcaatc cgtccttgaa cagcccgcgc agctggcccc
     5461 gctcgcgcac cagcacgccc acacccagga tcagatacag gatgccgacg gcgatccgct
     5521 ccacgccgtg gagggactcc ggcacgatcc cgcccaacag gaactggaac cagaacagcc
     5581 cgaacaggat gccagcctcg cgcaacgaca tcgacaggct cgagatcacc gccaccgcga
     5641 agaacgactg cgccgccgtg aggaacagct cctctcgctg cacggtgtcg agcggcaatc
     5701 catggaatcc gcccgccgcc accgcgaaca cgatcggcag cgtccccacc aggagggtcc
     5761 actggttcac cttggagctg accagcgtcc cgagacccgc gctggtgttg agccgccagg
     5821 cgtacaggcc cgccaccaac agttccggtg cttcggaggc gagcggtgcg atccattgca
     5881 ccaacaggaa ctcgctcacc cccagggtct cgccgctggc gacgagggcc tcggcgaaat
     5941 gctcggcgct cgccaggatc agcccggcgg cgaacaccgc gatcgtgatc accgtcgtgc
     6001 gtctggccgc ggtcgagaag gtcccaaggt agcgggccgg ccccacgagg tggggttcct
     6061 cggcgggggc acgcgagacc cgcacggtat acagcacgaa gatcgcgacc aggatcaccg
     6121 agtcgaacaa cgtcaccgaa tgcttcagcg gcagggtcag ggagtagatc gtggcgatcg
     6181 ccaggaacgc gatctcgatc gagtgctcgc gatcgagctt cacgcctccc cagtgctgtc
     6241 ccttgacgat cctgcccttg gcgcggagcc gccagaacgc gacgaacacc accatggtcc
     6301 agccgatgcc gatcaacagc cggttcgccc cggtcatgtt cgccaacgcc agcgagcacg
     6361 gcgactcggt gccgccgtcc agcggcaggc acgccgcgcc ggtctccgcg aacgattcgc
     6421 cgcccttgcg ggcgaacacg aagtccacgg cgtactcggg cagcaccgcg atcagcgcca
     6481 gccccgccag ggcgagcgtg cgcggcacat cgcgctcagc cgcctcagca acccacgtga
     6541 ggatgaacgc cgccgccacg atcgc
//
LOCUS       DATDBZ010000164         6778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1057675, whole genome shotgun sequence.
ACCESSION   DATDBZ010000164 DATDBZ010000000
VERSION     DATDBZ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057675"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VIX62_03995"
     CDS             <1..894
                     /locus_tag="VIX62_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY5859424.1"
                     /translation="DLWVAHGMPAPPSFPHVGGADGAGVIDATGPGVTDAEVGDEVVI
                     DPSISCGRCPACARGDVPFCPDFAIVGEHRWGTHADAIVLPEVNVARKPPALSWPEAA
                     AVGLVTSSALRMLRRGRVDEHTSVLVTGIGGGTSTAAFLLARAMGATVFATSRDASKL
                     AWATDHGARAVFDSDAAFDDQARAATDGRGVDVVLDNVGSAVFDRALRALARGGRYVT
                     NGSTTGRTAELHLPTLFWRQLEVIGSTMNDHREFADAMAFVGGGVPHVPVDREFAFEE
                     FPEALARLDAGEQLGKIVLTR"
     gene            1015..1602
                     /locus_tag="VIX62_04000"
     CDS             1015..1602
                     /locus_tag="VIX62_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5859425.1"
                     /translation="MPDERTDSELLLASRDNPDAFTELYRRHAEDLLRYFARRTLDPE
                     TAAELTAETFAEAFASRQRYTDQGVNGVAWVYGIARHRLGRFFRTGRVDAAARQRLGM
                     PERDLPPEDYERIEDLIDFAPIREALLEALETLSEDQREAMRLHVIDGLGYREVAERL
                     RCSEANARQRVSRGLRRVALVLQERGLQLSSEVES"
     gene            1599..2744
                     /locus_tag="VIX62_04005"
     CDS             1599..2744
                     /locus_tag="VIX62_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY5859426.1"
                     /translation="MNTDIRYLQQLETDFEHVAARERRRTAAEPSGRGGRGSRPPRRP
                     THRDRRWPSIAAAVVTLLIVAGSIGFLSQQADMSSDDSGGADALAPAEAPVDGPATWQ
                     ARNPNASAAPAPGQALTDGDTSAAADEQGGRSLDYSVGDTKTPAGIGTTPQAQGDLSK
                     IIRDGRIEVVVPTGEFAKNVTAVTRIAGTNGGFVLTSSTQNGQAGQFTLRIPAKRFDR
                     AMDQLRGLGTVQSDAVTGDDVTAEFVDLQARLGILTDRRDLLRDLQSEANSSSEILRF
                     ATLIDQVQLEIENIQGQLNFIRDQVAEGTIKVSLREIGAPEAQEQQPTDVDKPSLGTA
                     FNLALQGFLRVLGAVVVGLGYLIPIGVIALIAWFVVRLVRRRDREAS"
     gene            complement(2707..3273)
                     /locus_tag="VIX62_04010"
     CDS             complement(2707..3273)
                     /locus_tag="VIX62_04010"
                     /inference="COORDINATES: protein motif:HMM:NF025460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4272 domain-containing protein"
                     /protein_id="HEY5859427.1"
                     /translation="MSRPPVAEVSDRALVLYALSRRGAIEFVVTESGGEPSRLAQAEH
                     ARIEAERWLERESVTPALRDDERALMDAESGSWPAEAIADAMWRKEALGVLLWALEHL
                     AELPPYGEEFQQQVVDEAIMRYGSVDAFRANGRMRPDDQIEQAWMESDAWFGATEGRQ
                     GDDATMASIAAERFRALSWLRDRDAAPA"
     gene            complement(3291..3887)
                     /gene="recR"
                     /locus_tag="VIX62_04015"
     CDS             complement(3291..3887)
                     /gene="recR"
                     /locus_tag="VIX62_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY5859428.1"
                     /translation="MAYEGPIQELIDQLARLPGVGPKSAQRLAFWLVKADPEDARRLA
                     AAIIQAKERITFCRECGNVAEGDLCRICRDESRDRTLLCVVEEPKDAASIEKAALIKG
                     RFHILGGAISPLDGIGPDDLRVQELLDRVDRDHVTEVILATNPNLEGNATAMYVAALI
                     KPLGVRVTRLASGLPVGGDLEYADEVTLSQALDGRREM"
     gene            complement(3890..5704)
                     /gene="dnaX"
                     /locus_tag="VIX62_04020"
     CDS             complement(3890..5704)
                     /gene="dnaX"
                     /locus_tag="VIX62_04020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY5859429.1"
                     /translation="MADEIQHQALYRRYRPQTPQEVLGQDHVIRALTGAIREGRLHHA
                     FLFCGPRGTGKTSTARILAKMVNCEQGPTAEPCGVCSQCVAIREGTHLDVVEIDAASH
                     GGVEDARELREKAPTAPVQGREKVYIIDEAQRLSREAFDALLKVFEEPPPGVRFVLAT
                     TEPFKMPATIVGRSQRFDFRRFGLETLTGQLQTIAAAEGTSLTESAAHSIARQAEGSS
                     RDALSLLDQARVLGGDKIDDDVVHALLGAPRGEVQHELADSIAVGDARGAFEIVGRLV
                     ADGQDVRNVTNESLAHFRNLLLVKTAPGQQDLLDVPDDAYDQLRIQAEKFSPAEITRV
                     IALLLAAQNDMRWTTSPRLTLELALIRATLPDTDPAPAGLVARLERLERLAQLDPTAV
                     VPTAADVDHAAGPVSPADDRAPVEAVEPPVVDEAPATSAPSVDPAPSEPEPRKPDPPK
                     APQPPQPSQQMPVPVAADAGNIDVAMLRRSWPQLIDHLANNRQMILKAILESATVASY
                     DGTTIELAFPPDRKVGPQKVEEKQEDLRSALGDLFGIKPRVMCVVREFREPAGEPPIV
                     EIVDEADVPDEAEALRRVQEMLGAQPVDEDKDDEKGEG"
     gene            complement(5759..6024)
                     /gene="ffs"
                     /locus_tag="VIX62_04025"
     ncRNA           complement(5759..6024)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIX62_04025"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            6145..6741
                     /locus_tag="VIX62_04030"
     CDS             6145..6741
                     /locus_tag="VIX62_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859430.1"
                     /translation="MRTPARSRWLIALVAAVAVVSIATVAAATRGGSEQEHANDGSGQ
                     VSAGSPGDPNGSVAIDPVHGDCNVAPLDRNADPDSPVDYAVCPDDQDPAPWGDPSTAQ
                     PVEPTPGMAGVRARPFDRAMLNDDGTVTILFTSGVEPCAVLDHVTVDEGDAVTITLYE
                     GHDPDAGDVACIEIAVLKSVTITLDHPLDGRSIVDGAA"
BASE COUNT         1016 a         2396 c         2326 g         1040 t
ORIGIN      
        1 gacctatggg tcgcgcacgg catgccggcc cctccctcgt tcccgcacgt cggtggcgcg
       61 gacggtgccg gggtgatcga tgcgaccggc ccgggtgtca cggacgccga ggtgggcgac
      121 gaggtggtga tcgacccgtc catctcgtgc gggcgatgcc cggcgtgcgc ccggggcgac
      181 gtgccgttct gtccggactt cgcgatcgtg ggcgagcatc gctggggcac ccacgcggac
      241 gccatcgtgc tccccgaggt caacgtcgcc cggaagcctc cggcgctgtc ctggcccgaa
      301 gcggccgccg tcggcctggt cacctcctca gcgctccgca tgctccgacg cggacgggtc
      361 gacgagcaca cctcagtcct cgtcaccggg atcggcggcg gcacgtcgac cgcggcgttc
      421 ctcctcgccc gggcgatggg cgccacggtg ttcgccacct cgcgcgacgc ctccaaactg
      481 gcgtgggcca ccgaccacgg tgctcgcgcg gtgttcgatt cggatgcggc cttcgacgat
      541 caggcgcgag cggcaaccga tggacgtggc gtcgacgtgg tgctcgacaa cgtgggctcg
      601 gcggtcttcg atcgggcatt gcgggcgctc gcccgaggcg ggcgctacgt gacgaacgga
      661 tccacgaccg ggcgcacggc cgaactccac ctgcccacgc tgttctggcg ccagctggag
      721 gtgatcggct ccacgatgaa cgaccatcgc gagttcgccg acgcgatggc cttcgttggg
      781 ggcggcgtgc cccacgtccc ggtggatcgc gagttcgcgt tcgaggagtt cccggaggcg
      841 ctggcgcgcc tggatgccgg cgaacaactc ggcaagatcg tgctcacgcg ctagggtccg
      901 ggaacctcgg ggacgtcacg gtgcgtccga gcgctgtcac acccccaccg cctcgcggca
      961 tggtccctac gacacggacc gatcgtcgcg aggacggtcg atcagggagg ccccgtgccg
     1021 gacgagcgaa ccgacagcga actcctgctc gcgtcgaggg acaaccccga tgcgttcacg
     1081 gagttgtacc gacgccatgc agaggatctg ctgcgctact tcgcccgccg cacgctcgac
     1141 ccggaaacgg cggccgagct gaccgcggag accttcgccg aggcgttcgc gtcgcggcag
     1201 cgctacacgg atcaaggagt gaacggggtg gcatgggtgt acgggatcgc gcggcaccgc
     1261 ctcgggcggt tcttccgcac cgggcgggtc gatgcggcgg cccgccagcg gctcgggatg
     1321 cccgaacggg acctgccgcc cgaggactac gaacgcatcg aggacctcat cgacttcgcg
     1381 ccgatccgcg aggcgctgct cgaagcgctg gagacgctga gtgaggacca gcgtgaggcg
     1441 atgcgactgc acgtgatcga tggactcggc taccgcgagg tcgccgagcg actccggtgc
     1501 tccgaggcga acgcgcgaca acgcgtgagc cgtgggctgc gccgcgtcgc actggtgctg
     1561 caagagcggg ggctgcagct gagctcggag gtcgaatcgt gaacacggac atccgctacc
     1621 tgcagcagtt ggaaaccgac ttcgagcacg tcgccgcacg ggaacgccgg cgtactgccg
     1681 ccgagccctc cggccgcgga ggtcgagggt cgcggccgcc gcgccggccc acccatcgcg
     1741 atcgtcgctg gccctcgatc gcggcggccg tggtcacgct gttgatagtg gcgggcagca
     1801 tcggcttcct cagccagcag gccgacatga gttccgacga cagcgggggc gctgatgctt
     1861 tggcgccggc cgaagccccg gtggacggtc ccgcgacatg gcaggcgagg aaccccaacg
     1921 catcggccgc gcctgcaccc ggccaagcgc tgaccgacgg tgacacgagc gctgccgcgg
     1981 acgagcaggg cggccgttcg cttgactatt cggtcggtga cacgaaaacg ccggccggga
     2041 tcgggacgac cccacaggcc cagggcgacc tgtcgaagat catccgcgac ggtcgcatcg
     2101 aggtcgtcgt tccgacgggc gagttcgcca agaacgtcac cgccgtcacc cgcatcgccg
     2161 gaaccaacgg cgggttcgtc ctcacctcgt ccacgcagaa cggacaggcg ggacagttca
     2221 cgctgcggat cccggccaag cggttcgacc gggcgatgga tcagctccgt gggctcggca
     2281 ccgtgcagtc cgacgcggtc accggcgacg acgtcacggc cgagttcgtc gatctgcagg
     2341 cacggctcgg catcctcacc gaccggcgcg acctgctgcg cgacctccag tccgaggcga
     2401 actcgtcctc cgagatcctt cggttcgcca cgctcatcga tcaggtgcag ctggagatcg
     2461 agaacatcca gggtcagctc aacttcatcc gcgaccaggt ggccgagggc acgatcaagg
     2521 tctccctccg tgagatcggc gcgcccgagg cccaggaaca gcagcccacc gacgtcgaca
     2581 agccgagcct cggtaccgcg ttcaacctgg cgttgcaggg attcctgcgg gtgctcggcg
     2641 cggtcgtcgt gggcctgggc tacctgatcc cgatcggcgt gatcgccctg atcgcgtggt
     2701 tcgtggtcag gctggtgcgg cgtcgcgatc gcgaagccag ctgagcgcgc ggaaccgttc
     2761 ggcggcgatc gaggccatcg tggcgtcgtc gccctgacgc ccctcggtcg ccccgaacca
     2821 cgcgtcggac tccatccagg cctgctcgat ctgatcgtcg ggacgcatgc gtccgttcgc
     2881 ccggaaggcg tcgaccgacc cgtagcgcat gatcgcttca tccaccacct gttgctggaa
     2941 ctcctcgccg tacggcggga gctcggccag atgttcgagc gcccacagca gcacgccgag
     3001 ggcctccttg cgccacatcg catcggctat cgcctcggcg ggccaggacc ccgactcggc
     3061 gtccatcagc gcgcgttcgt catctcgcag tgccggcgtc actgactcac gctcgagcca
     3121 ccgttcggct tcgatacgag cgtgctcggc ctgcgccagc cgcgacggct ctcctccgct
     3181 ctcggtcacg acgaactcga tcgcgccgcg ccgggacagc gcgtagagca cgagcgcgcg
     3241 gtcggagacc tcggcgacgg gtggccggct caacgtggct gtctccgagg tcacatctct
     3301 cggcggccgt cgagcgcctg ggagagcgtg acctcatcgg cgtattcgag atcgccgccc
     3361 acagggaggc cgctggccag ccgcgtgacg cgaacgccca acggtttgat cagcgccgca
     3421 acgtacatgg cggtggcgtt gccctcgagg ttcgggttgg tggccaggat gacctcggtg
     3481 acgtggtctc ggtcgactcg gtcgagcagc tcctgcaccc ggagatcgtc gggaccgatg
     3541 ccgtcgagcg ggctgatggc gccgcccagg atgtggaacc ggcccttgat cagggctgcc
     3601 ttctcgatgc tggcggcgtc cttcggttcc tcgaccacgc acagcagcgt gcggtcgcgc
     3661 gactcgtccc ggcagatgcg gcacaggtca ccttcagcga cgttgccgca ttcccggcag
     3721 aacgtgatgc gttccttcgc ctggatgatc gccgcggcca gtcgtctcgc gtcctcgggg
     3781 tcggccttca ccagccagaa cgcgagccgc tgcgccgact tgggacccac gccgggcagc
     3841 cgagcaagct ggtcgatcag ctcctggatg ggaccttcgt acgccacggc taaccctcgc
     3901 ccttctcgtc gtccttgtct tcgtcgacgg gctgggcccc cagcatctcc tggacgcggc
     3961 ggagcgcctc ggcttcgtcg ggcacgtcgg cctcatcgac gatctccacg atgggcggct
     4021 cgccggccgg ctcccggaac tcccgcacca cgcacatgac ccgcggcttg atgccgaaca
     4081 gatcgcccag cgccgaccgg aggtcctcct gcttctcctc caccttctgc ggacccacct
     4141 tgcgatcggg cgggaaggca agctcgatcg tcgtcccgtc gtaggacgcc acggtggccg
     4201 actccaggat ggccttcagg atcatctgcc ggttgttggc caggtggtcg atcagctgag
     4261 gccaggaacg ccggagcatg gcgacatcga tgttgcccgc gtcggcggcc acgggaaccg
     4321 gcatctgctg cgagggctgg ggcggctgtg gcgccttcgg cggatccggc ttccgaggtt
     4381 cgggctcgct cggcgcggga tccacggacg gcgccgaagt agcaggcgcc tcatcgacga
     4441 cgggcggctc cacggcctcc accggcgccc ggtcgtcggc cgggctgacc ggaccggcgg
     4501 cgtgatcgac gtcggccgcc gttggcacga cggccgtggg atcgagctgc gccaaacgct
     4561 ccagtcgttc gagcctcgcc accaggccgg ccggcgcagg gtcggtgtcg gggagcgtgg
     4621 cacggatcaa cgccagttcc agggtgaggc ggggcgacgt ggtccaccgc atgtcgttct
     4681 gcgcggccaa caacagcgcg atcacccgcg tgatctcggc cgggctgaac ttctcggcct
     4741 ggatccgcaa ttggtcgtac gcgtcgtcgg gcacatcgag gaggtcctgc tggcccggcg
     4801 cggtcttgac caggagcagg ttccggaagt gagccaagga ctcgttcgtg acgttgcgaa
     4861 cgtcctgacc atcggcgacc agccgtccga cgatctcgaa tgcgccgcgg gcgtcgccca
     4921 ccgcgatcga atcggccaac tcgtgctgga cctcaccgcg gggcgcgccc agcagtgcgt
     4981 gcacgacgtc gtcatcgatc ttgtcgccgc ccagcacgcg ggcctggtcg agcagcgaaa
     5041 gcgcgtcgcg cgaggaccct tcagcctggc gagcgatcga gtgggccgcc gactccgtga
     5101 gcgaggtgcc ctctgccgcg gcgatcgtct ggagctgccc ggtgagggtc tccagcccga
     5161 accgacggaa gtcgaagcgc tgcgaccgtc cgacgatcgt ggccggcatc ttgaacggtt
     5221 cggtggtggc aagcacgaac cgtacgccgg gcggcggttc ctcgaagacc ttcagcaggg
     5281 cgtcgaacgc ctcgcgggag agccgctgcg cctcgtcgat gatgtagacc ttctcgcggc
     5341 cctgcacggg cgcggtgggc gccttctccc gcagttcgcg agcgtcctcc accccgccgt
     5401 ggctggcggc gtcgatctcg acgacgtcca ggtgcgtgcc ctcacggatc gcgacgcact
     5461 gggaacacac gccgcacggc tcggccgtgg gcccctgctc gcagttgacc atcttggcca
     5521 ggatgcgagc ggtggaggtc ttgcccgtcc cccggggccc acagaagagg aacgcgtggt
     5581 gcaggcgccc ctcgcggatg gcgcccgtca gcgcacggat cacgtggtcc tgtcccagga
     5641 cctcctgggg ggtctgcggc cggtaccgcc ggtacagggc ctggtgctgt atctcgtccg
     5701 ccactcacgc ctccatggcc acgggccgcc cacccggaca cccgggtgaa agatgtggcc
     5761 gtgcacccgc tgtcgaccac ggatgactcg ggcgcttctc ggcgcggtag ctccagccag
     5821 gcatccctac ggcacccatc aagctcccct tacggctgct tccttccgga cctgaccggg
     5881 ttcgaggtct gatgtcgcgt aggacccgac tttcaacgct ccgcactcaa ggctgccccg
     5941 gaccttccgg gcctcgagcg gggattcggc cccgctgaag cggattgcgg gtgcagggca
     6001 ccgctagctc cccgcctagc acggccacgc ggggagtata cgcagccccc aggacaacgc
     6061 ttcagacggg aacctgaggg ctcgatgctt cgtccaacac ccgttgcgcc ccggccatat
     6121 ggcctcgaag ctcggaggaa ccggatgcga acccccgctc gatcccgctg gttgatcgcc
     6181 ctcgtcgcgg ccgtcgcggt ggtctccatc gccaccgtcg ccgcagccac acgaggtggc
     6241 tcggaacagg aacacgcgaa cgatgggagc ggccaggtgt ccgctggttc gcccggcgac
     6301 ccgaacggtt cggtggcgat cgacccggtc cacggcgact gcaacgtcgc gccgctggac
     6361 cggaacgccg acccggattc cccggtcgac tatgccgtgt gtccggacga ccaggacccg
     6421 gcaccatggg gcgacccgag caccgcccag ccggtcgaac cgacgcccgg tatggccggc
     6481 gtgcgagccc gcccgttcga ccgcgcgatg ctcaacgacg acggcacggt gaccatcctg
     6541 ttcaccagcg gcgtggagcc gtgcgcggtg ctcgatcacg tcaccgtcga cgaaggtgat
     6601 gccgtcacga tcacgttgta cgaaggccat gacccggacg ccggcgacgt cgcctgcatc
     6661 gagatcgcgg tcctgaagtc ggtgacgatc acgctcgacc acccgctgga cggccgcagc
     6721 atcgtggacg gtgccgccta agaggccgcg cgtatccaga tctcgtggtc ggccacag
//
LOCUS       DATDBZ010000165         7466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1057929, whole genome shotgun sequence.
ACCESSION   DATDBZ010000165 DATDBZ010000000
VERSION     DATDBZ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057929"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..646)
                     /locus_tag="VIX62_04035"
     CDS             complement(2..646)
                     /locus_tag="VIX62_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5859431.1"
                     /translation="MLVDDHEIVRDGIKGMLDTQDDIVVTAQAGSVQEAVDEAARTRP
                     DVVVMDVRLADGSGIEATRQIRADHPATKVLMLTSFADDEALFSSIMAGASGYVLKQV
                     KPGELLRAIRAVGAGDSLLDPGVTTAVLDRLRKGKHLLKDEKLARLSAQEERILTLVA
                     DGRTNREIGDELHLAEKTVKNYVSSILSKLEVARRAEAAAYLARHTTTPGSVEE"
     gene            complement(683..1165)
                     /locus_tag="VIX62_04040"
     CDS             complement(683..1165)
                     /locus_tag="VIX62_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859432.1"
                     /translation="MFARYFIELPMSPEQVTAALARHPEEWVPGLAEQANHVGDTLLA
                     DVGFGEHLRIARTVVLELGEPIAMATKTLIPLRWEATGPRGVFPSMEADLEIAPLSAD
                     RTQLAMSARYVPPMGAVGRAMNRTMLFRVAEATLKDFLDGVGRALMSPTAGASAGTAI
                     "
     gene            complement(1261..2403)
                     /locus_tag="VIX62_04045"
     CDS             complement(1261..2403)
                     /locus_tag="VIX62_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY5859433.1"
                     /translation="MGEAERQVDRYALLIDAGLALASELDLEAVLERIVELAIEITDA
                     RYGALGVLGESTPRIERFITKGISDQDRAAIGHIPVGEGILGLLIRERRPMRIPDIGA
                     DPRAHGFPPNHPPMHSFLGAPVIALGLVFGNIYLTEKQGASEFSDEDEAALVVLAIQA
                     GVAIENARLYAEKELAQRELARLELLEERERIAKELHDGVIQSLFAVGMGLQGTAAMS
                     PDEEIGRRLEGAVEEIDRAIRDLRNYIFGLRPGILADRQLGQALEELGHEFSDRSGVL
                     TVIDIDGSVAAELAGLAGDLIQVVREALSNVGRHADATSVRVTLRRIDTGAELEIDDD
                     GAGFDVDSPAGMGLANLRDRVQAIGGELAVESFPGEGTTVRARLPL"
     gene            complement(2460..3053)
                     /locus_tag="VIX62_04050"
     CDS             complement(2460..3053)
                     /locus_tag="VIX62_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY5859434.1"
                     /translation="MTNWNLLRAAGIGAYLMLYLSVAWGLVATTSIVTKRISKVSSNL
                     FHQFVATTGLVLLGVHLALLLIDGFMPFAPLDLILPLRSAYRPIPIALGIAAMFAMVA
                     IMVSSWLRKPIGVRLWRAIHLAAVPAFALALGHGVFAGTDTNRPWMAAMYAFTGLSVV
                     FLTIVRGLTYGYRPPRAERPVPARGVTTKDPEPASTS"
     gene            complement(3050..3952)
                     /locus_tag="VIX62_04055"
     CDS             complement(3050..3952)
                     /locus_tag="VIX62_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5859435.1"
                     /translation="MQPITTLRAEARRRDTFRAMGTEVTLYGPDDDAFGEAFTTVGRV
                     FETEERRFSRFRDDSELTAVNRLAGRWVPVSTPFERVVRLALDQASATGGLFDPTALH
                     AVMAVGYDRDFDEVLARARGALHPPEPCGRWNEVEVRPGAIRLPPGVGLDLGGIAKGW
                     TADLAAAGALDAGLAWALIAAGGDLAIVGDAPLLEIPIEDPETLEGSMATLRLDRGAL
                     ATSSVAKRSWGPGLHHVIDPRSGAPLRSDTVQATVWAPTCAEAEVLATWALLRGPDAA
                     TQLPCALVLSSGDVTVSFDQEMIP"
     gene            complement(3955..4323)
                     /locus_tag="VIX62_04060"
     CDS             complement(3955..4323)
                     /locus_tag="VIX62_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859436.1"
                     /translation="MTEQRPSAKPKRWSKGRVRALAWVTGTATFLAGFGILGAAPKPS
                     TANATDSSNRQKPPRQRIIVRKVTRRVVVVDPAVTAPVTFVPTTSGGGSSTGGGTSSG
                     GGTTTAPPPPPPPPPPTSGS"
     gene            complement(4320..4763)
                     /locus_tag="VIX62_04065"
     CDS             complement(4320..4763)
                     /locus_tag="VIX62_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859437.1"
                     /translation="MNKRSAMLMAAGLVLTLIVGGVAMSIGLTGPTSSEATPRAERRR
                     TAAPIVKTVKHTVTVHKRGSAAPATGTGTSGSNSPGSVAGSTDDDHGSDDDDDVHGED
                     HGDHGSDDSDDHGDDHGDHGGHGGDDHGDDDQDHDVHGDDDRTAS"
     gene            complement(4918..5646)
                     /locus_tag="VIX62_04070"
     CDS             complement(4918..5646)
                     /locus_tag="VIX62_04070"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY5859438.1"
                     /translation="MTRDHTTIEELLAVQALGGLDGDDVQTLAAERASHGDCEECARL
                     EAEFSETAGRLAFSLEPVPVDDAMADRILAESPASPVVAPEPATTVDDLGERRERRVR
                     GWQAAVGAAAVIAVLLVAVAVIRVDRTTAIAGASTTQRLVTFSGSGEGELAMAFTPGE
                     SGVVLWGSDLPDPGQDKVYEIWMIQDGTPVSGGCFRPQDGQVALSVDASLEGTDAMAV
                     TAEPADCPSSPSGEPVKTANLTLD"
     gene            complement(5643..6215)
                     /locus_tag="VIX62_04075"
     CDS             complement(5643..6215)
                     /locus_tag="VIX62_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5859439.1"
                     /translation="MIDISEARDRDLLLRLRDRDEDAFRGLFARYAPTAKALAQRVVR
                     QSHLAEEIVQEAFMAVWRNPEGYDAERGSVKSWLMGMVHHRAVDLVRREESYRRRAED
                     SIPEAMQEQVDHADEVVQQVGLPEERRIVRAALDELPAEQRDVLTLMYFEGMSQSQIA
                     ERTGLPLGTVKSRTLLGMRRMRASLEGIER"
     gene            complement(6322..7272)
                     /locus_tag="VIX62_04080"
     CDS             complement(6322..7272)
                     /locus_tag="VIX62_04080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5859440.1"
                     /translation="MAVLFLWRNDDGAPHVSAVLGWDNPPTQQPNGPEGRPIDVVTVV
                     LVGLEDSLSEELAGEEGFDVQRTGDIEGLDEHRGADAVLIALEGSGPLEVLGALRARA
                     PEAAVLVVTGPDREADGAVALHGGAEDHLVRGSIPDGFLSRAIRYAIAQRRLRRELST
                     TDEITGLPNLRGFAPIAEHHLRMADRAKTQVVFVFMRLDGLQAARAGSPEEAEVLVRE
                     AANVLLEAVRDADVPARITEDTFAVLLTGDAVGAETLVLSRLVEAIAVHDTDRQSPRS
                     LSLSVGSALYDPEEPATLSQILETAGRRLGDQRPEGSSKA"
BASE COUNT         1112 a         2613 c         2579 g         1162 t
ORIGIN      
        1 gctactcctc gacggagccg ggcgtggtgg tgtggcgggc gaggtaggca gcggcctcgg
       61 cgcgccgggc gacctccagc ttcgacagga tcgagctgac gtagttcttc acggtcttct
      121 cggccaggtg caactcatcg ccgatctcgc ggttcgtgcg gccgtcggcc accagggtca
      181 ggatgcgctc ttcctgcgct gagagccggg cgagcttctc gtccttcagc aggtgcttgc
      241 ccttgcgcaa gcggtccagc actgcggtgg tgacgccggg atccagcagc gaatcgccgg
      301 cgccgacggc gcggatcgcc cgcaacagct cgcccggctt cacctgtttc agcacatagc
      361 cggaggcgcc ggccatgatc gaggagaaca gcgcctcgtc atcggcgaac gaggtcagca
      421 tcaggacctt ggttgcagga tggtcggcgc ggatctgccg ggtcgcctcg atgccgctgc
      481 cgtccgccag tcgcacgtcc atcacgacga cgtcggggcg agtgcgcgct gcctcgtcga
      541 ccgcttcctg cacgctgccc gcttgcgcgg tcaccacgat gtcgtcctgc gtatcgagca
      601 tccctttgat gccgtcgcgg acgatctcat gatcgtccac gagcatcaca cggagcggag
      661 ccggcttcgt cacgcggcca tcctagatgg cggtgccggc ggaagctccc gccgtcggcg
      721 acatcagcgc gcgccccacc ccgtccagga agtccttgag cgtggcctcg gcgacccgga
      781 agagcatcgt gcggttcatc gcgcgtccca cggcgcccat gggcggaacg tatctggcgc
      841 tcatggccag ctgcgtcctg tccgcactca acggcgcgat ctccaggtcg gcctccatcg
      901 acgggaacac gcctctcgga ccggtcgcct cccagcgcag ggggatcagc gtcttggtgg
      961 ccatcgcgat gggttcgccg agttcgagga cgaccgtgcg tgcgatccgg aggtgttctc
     1021 cgaaccccac gtcggccagc aacgtgtcgc caacatggtt ggcctgctcg gcgagcccgg
     1081 ggacccactc ctcagggtga cgggccaggg cggcggtcac ctgctccggg gacatcggca
     1141 gttcgatgaa gtagcgcgcg aacacctctc cagtgtcggt tcgccgccgt gcgacgacag
     1201 gggacggacg accctgccgc ttggggccgg tcggcacccc cgtgggagca ggggagcgat
     1261 tcacagcggc agtcgagctc gcaccgtggt gccctctccc gggaagctct ccacggcgag
     1321 ttcgccgccg atcgcctgaa cccggtcgcg gaggttggcc aggcccatcc ccgcggggct
     1381 gtcgacgtcg aagccggcgc cgtcgtcgtc gatctcgagc tcagcgcccg tgtcgatgcg
     1441 gcggagcgtg acgcggacgc tggtcgcgtc cgcgtgccgg cccacgttcg acagcgcctc
     1501 gcgcaccacc tggatcaggt cgcccgccag cccggcgagt tcggcggcga ccgagccgtc
     1561 gatgtcgatc acggtgagca cgccggatcg gtcgctgaac tcgtgaccga gctcctcgag
     1621 cgcctggccg agctggcggt cggcgaggat gcccggccgg agcccgaaga tgtagttgcg
     1681 gaggtcgcgg atcgcgcggt cgatctcctc gacggcccct tcgagccgcc ggccgatctc
     1741 ctcatcgggc gacatcgcgg ccgtgccctg caggcccatg cccacggcga acagcgactg
     1801 gatcactccg tcgtgtagct ccttggcgat ccgctcacgt tcctccaaca gttccagtcg
     1861 agcgagctca cgctgggcca gctccttctc ggcgtagagg cgtgcgttct cgatggccac
     1921 tccagcctgg atcgccaaca cgacgagggc ggcctcgtcc tcatcgctga actccgaggc
     1981 gccctgcttc tcggtcaggt agatgttgcc gaacaccagg ccgagcgcga tgacgggcgc
     2041 ccccaggaac gagtgcatcg gcggatggtt cggcgggaag ccgtgcgctc gcgggtcggc
     2101 gccgatgtcg gggatccgca tcggccgtcg ctcgcggatc aggagaccga ggatgccttc
     2161 acccaccggg atgtggccga tcgctgctcg gtcctggtcg ctgatcccct tcgtgatgaa
     2221 gcgttcgatc cgaggcgtgg actcgccgag cacaccgagc gcgccgtacc gggcgtccgt
     2281 gatctcgatc gccagctcca cgatgcgttc gagcaccgcc tcgaggtcga gttctgaggc
     2341 cagggccagt ccggcatcga tgagcagcgc gtatcggtcg acctgccgtt ccgcctctcc
     2401 cacgcgcgaa gcctagccaa cgcgcgcggc ggccgcgacc tgagctcgtg ataccgcgat
     2461 caactggtcg aggcgggttc aggatccttg gtggtgaccc cccgagcggg aaccgggcgc
     2521 tcggcccgag gcggacggta gccgtaggtc aggccgcgca cgatcgtgag gaacacgacc
     2581 gaaagtcccg tgaacgcata catcgcggcc atccacgggc ggttcgtgtc cgtccccgcg
     2641 aatacgccgt ggcccagcgc cagggcgaag gcgggcacgg ccgccagatg gatcgcccgc
     2701 cacagccgca cgccgatcgg cttgcgcagc cacgacgaga ccatgatcgc caccatggcg
     2761 aacatcgccg cgatccccaa ggcgatcggg atcggacgat acgccgagcg cagcggcagg
     2821 atcaggtcga gcggcgcgaa gggcatgaat ccgtcgatca gcagtagcgc caggtggacg
     2881 ccgagcagga cgaggccagt cgtcgccacg aattgatgga acaggttgga cgacaccttg
     2941 gagatccgct tcgtgacgat cgaggtggtc gccaccagac cccaggccac cgagaggtag
     3001 agcatcaggt aggcgccgat cccggcggca cgcagcaggt tccagttggt cacgggatca
     3061 tctcctggtc gaagctcacg gtgacgtcgc cactcgaaag gacgagggca cacggaagct
     3121 gcgtggcggc atcgggcccg cgcaacagcg cccaggtggc caacacctcg gcctcggcgc
     3181 acgtcggcgc ccacacggtg gcctgcacgg tgtcggatcg caacggcgca cccgaccggg
     3241 ggtcgatcac gtggtggagg cccggacccc acgagcgctt ggcgaccgac gaggtggcca
     3301 acgcccctcg atccagtcgg agcgtggcca tcgatccttc gagggtctcc gggtcttcga
     3361 tggggatctc cagcaaggga gcgtcaccca cgatcgcgag gtcgccaccg gcggcgatca
     3421 gcgcccacgc gagaccggca tcgagtgcgc ccgcggcggc gaggtccgcg gtccacccct
     3481 tcgcgatgcc gccgaggtcc aggcccaccc cgggcggcag ccggatggcg ccgggccgca
     3541 cctccacctc gttccagcgt ccgcacggct cggggggatg gagcgctcct cgcgccctgg
     3601 ccaggacctc gtcgaagtcc cggtcgtagc ccacggccat cacggcatgc agcgcggtcg
     3661 ggtcgaacag cccacccgtc gcagacgcct ggtcgagggc gagccggacg acgcgttcga
     3721 acggagtcga caccggcacc caccggccgg cgaggcggtt cacggcggtg agctcgctgt
     3781 cgtcgcggaa ccgggagaac cgtcgctcct cggtttcgaa cacgcgtccg accgtggtga
     3841 acgcttcgcc gaaggcgtcg tcgtcgggcc cgtagagggt cacctcggtg cccatcgctc
     3901 ggaacgtgtc gcgacggcgt gcctcggctc gcaaggtcgt gatcggctgc atcgctacga
     3961 acccgatgtg ggtggtgggg gtggcggagg cggcggcggg gccgtggtgg ttccgccgcc
     4021 ggacgacgtg ccgccgccgg tggaggatcc accgcccgac gtcgtcggta cgaaggtcac
     4081 cggcgccgtg accgcagggt ccacgaccac cacgcgccgg gtcaccttgc ggacgatgat
     4141 ccgctgacgc ggcggcttct ggcggttcga ggagtcggtc gcgttcgccg tggaaggctt
     4201 cggagccgcg cccaggatcc cgaaccccgc gaggaacgtg gccgtgccgg tcacccaggc
     4261 gagtgcccgg acgcggccct tgctccaccg tttgggtttc gcggaaggac gctgttcggt
     4321 catgaggccg tccggtcgtc gtcgccatgg acgtcgtggt cctggtcgtc gtcgccatgg
     4381 tcgtcgccgc catggccccc gtggtcgcca tggtcgtcgc cgtggtcgtc cgagtcgtcg
     4441 cttccgtgat cgccgtggtc ttcgccatgg acgtcgtcat cgtcatcgct gccgtggtca
     4501 tcgtcggtcg aaccggccac cgagccaggg gagttcgaac ccgacgtgcc ggttccggtc
     4561 gccggcgcgg cggagcctcg cttgtgcacg gtcaccgtgt gcttcacggt cttgacgatc
     4621 ggcgcagccg tacggcggcg ctcggcgcgg ggggtggctt cgcttgatgt cggtcccgtg
     4681 agtccgatcg acatcgccac gccacccacg atcaatgtca gcaccaggcc ggcggccatc
     4741 aacatcgcgg aacgcttgtt catcccatgc tcctcgtcgt cggttcttct cgcaccgacc
     4801 acgctaggca gcggcgtgct agggcttggg caaacgaggg ttaagggggc ggaaactctc
     4861 tgcagcgcag tggcgacctc tggagccccg aacgaggagg aggccaggag gagcggacta
     4921 gtccagggtc aggttcgccg tcttcacggg ttcgcccgac ggactcgacg gacaatctgc
     4981 cggttcggcg gtgacggcca tcgcatcggt gccttcgagg ctcgcgtcga ccgacagcgc
     5041 gacctgccca tcctgggggc ggaagcaccc gccggacacc ggcgtgccgt cctggatcat
     5101 ccagatctca tagaccttgt cctgacccgg atccggcagg tcggaccccc acagcaccac
     5161 gcccgactca cccggcgtga acgccatcgc gagttcgcct tccccgctgc ccgagaacgt
     5221 gaccagccgc tgggtcgtgg acgcaccggc gatcgccgtg gtgcggtcga cacgtatgac
     5281 ggcgacggcc accaggagca cggcgatgac ggccgcggcc cccacggccg cctgccaacc
     5341 gcgaacgcgc cgctcgcggc gttctccgag gtcgtccacg gtggtcgcgg gctcaggcgc
     5401 cacgacagga gaggcggggg actcggccag gatgcgatcg gccatggcgt catcgaccgg
     5461 caccggttcg agcgagaacg cgaggcggcc ggccgtctcg gagaactcgg cctcgagccg
     5521 cgcgcactcc tcgcagtcgc cgtgtgatgc gcgttcggcg gcgagggtct gcacgtcgtc
     5581 gccgtcgagc ccacccaggg cctgcacggc gaggagctcc tcgatcgtgg tgtggtcgcg
     5641 ggtcatcgtt cgatcccttc gagcgatgcc cgcatgcggc gcatgccgag gagggtgcgg
     5701 gacttcaccg tgcccagcgg cagtcccgtc cgctcggcga tctgcgactg ggacatgcct
     5761 tcgaagtaca tcaaggtcag gacgtctcgc tgctcggcgg gcagctcgtc gagggcggca
     5821 cgaacgatcc ggcgctcctc gggcaggccc acctgttgga cgacctcgtc ggcgtggtcg
     5881 acctgctcct gcatcgcctc cgggatcgaa tcctcggccc ggcggcggta ggactcctcg
     5941 cggcgaacca ggtcgacggc gcggtggtgg accatgccca tgagccagga cttcaccgag
     6001 cctcgctcgg cgtcgtagcc ctccgggttc ctccagaccg ccatgaacgc ttcctgcacg
     6061 atctcctccg cgagatgcga ttgccgtacg acccgctgcg cgagggcctt ggccgtgggc
     6121 gcgtagcgcg cgaacaagcc gcggaacgcg tcctcgtcgc gatcacggag gcggagcagc
     6181 aagtcgcgat cgcgcgcctc ggagatatcg atcatcccca ccgactcgct cctgaccagc
     6241 aacgacgtcc cctgttccca ccgcggccct tcggcgttcg tcccggatgc gggcgttctg
     6301 tcgcggggta ttcgcagcgc atcaggcctt ggatgacccc tccgggcgtt gatcgccgag
     6361 gcggcggccg gccgtctcga ggatctgcga cagggtggcg ggttcctccg ggtcgtagag
     6421 ggcactgccg accgacagcg agagcgaacg tggggactgc cggtccgtat cgtgcaccgc
     6481 gatcgcttcc accaggcggg acaagacgag cgtctccgcg cccaccgcgt cgccggtgag
     6541 cagcacggcg aaggtgtcct ccgtgatcct ggcggggacg tcggcgtcac ggactgcctc
     6601 gagcagcacg ttggccgcct cgcgcaccag gacctcggcc tcttccggac ttcccgcccg
     6661 ggcggcttgc agcccgtcga ggcgcatgaa gacgaagacc acctgggtct tggcgcggtc
     6721 ggccatccgg agatggtgct cggcgatcgg agcgaacccg cgcaagttgg gcaggccggt
     6781 gatctcgtcg gtggtggaca gctcacggcg cagtcgtcgt tgcgcgatcg cgtaccggat
     6841 cgctctcgag aggaacccgt cggggatcga gccacggacc aggtgatcct cggcgccgcc
     6901 gtgcagggct accgcgccgt cagcttcacg gtccggaccg gtcacgacca ggaccgccgc
     6961 ctcgggcgcg cgggccctca gggcgccgag cacctccagc ggaccagagc cttcgagtgc
     7021 gatgagcacg gcatcggccc cccggtgctc gtccaggcct tcgatgtcgc cggttcgttg
     7081 cacatcgaag ccttcttctc ctgcgagttc ctcgctgagg ctgtcttcca gcccgacgag
     7141 caccacggtg acgacgtcga tgggtctgcc ctccggtcca ttcggctgtt gcgtgggcgg
     7201 attatcccat ccgagcacgg cagatacgtg cggggctccg tcatcgttcc gccacaggaa
     7261 caacacggcc acgggggagc ctccacagag gctgcgccgg tacgcttggc gccatgaccg
     7321 atggaccctc gccggcgccc cctaccccga aaccgcacgg ccgcctgacc cactggttcg
     7381 tcggcatctc ggctgccctg tccctggtga tcgccgccgg cagcgcctac gggttcgtcg
     7441 cgtaccagca ggccaaaggc gaaggg
//
LOCUS       DATDBZ010000166         6952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1489023, whole genome shotgun sequence.
ACCESSION   DATDBZ010000166 DATDBZ010000000
VERSION     DATDBZ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489023"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VIX62_04085"
     CDS             <1..813
                     /locus_tag="VIX62_04085"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014876828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859441.1"
                     /translation="AWSLVPVFIAVAYSFNAGKAQTTWQGFSATRWFITDVNSVWQDE
                     TLRSALFQSLKLSALTVVIAVPLGVAFAIALDRWRGRGAGTSDFVMLFSFITPEIAIG
                     VGLFLMMTKLVEQIGLGFTAQVLGLSMYELAFTVIIVRARLLSVGKHYEEAAMDLGAS
                     PTNAIRRVLLPLLSPAIFASVAIVFASAIDNFVISQRLCADASCQTIPIVIYSSARRS
                     PLPSLNALATIVLVLSTVLIGAAVVGYRAMTRGERALAESPGAEAPVLASGG"
     gene            810..1643
                     /locus_tag="VIX62_04090"
     CDS             810..1643
                     /locus_tag="VIX62_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEY5859442.1"
                     /translation="MSEGRVRDLLRAGGDGNAILPGIDPRDTPGVDDRETAEAANPEL
                     ADRLDHLQERLWVEGKRSLLVVLQGMDTSGKGGTVRHVFSSMNPAGVDVASFKKPTDE
                     ELQHDFLWRIERRLPAAGQVMVFDRSHYEDVLIVRVRSLVPEAVWRPRYREIVEFEER
                     VAAAGTTIVKCMLHISYDEQRERLLARLDDPEKHWKFHEGDLEERRRWQDYQAAYEEA
                     IRECSTEAAPWFVVPSDRKWYRNWAISSLLVETLEDMDPQLPRPDLDVDALKGQLQPP
                     N"
     gene            1798..5421
                     /locus_tag="VIX62_04095"
     CDS             1798..5421
                     /locus_tag="VIX62_04095"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEY5859443.1"
                     /translation="MRRSTTLVIALSMAVSGLSLLPAAPAVAAGAPSTAQPTLVSADP
                     ADWTPNIQDGQVNAIVQVGNQVIVGGQFLQAKNISGNLLDVSSILAFNATTGTINKNF
                     MPTFDGEILALGTDGTSVYVGGRFGTVNGQTGYKRLVKLDPANGQIVTPFKAKIGAGE
                     AVHDMSFANGKLYIGGAFSAVQGVAREKFAALNPVSGALDPTVDVAFTGKHNGGTGRI
                     RKFDITPDGTKMVAVGNFTLVDGQDRDQAAMVDLTTDTVANWQTNRFKSACSSFFDTY
                     TNDIDIAPDGSYFVIGTTGAFMGGVNAGVLCDTTSRWEINATGTALQPTWVDYTGGDT
                     TFSVTATNTAVYVGGHQRWWNNPYSANGDSAGQGAIDRMGIAALDPLNGLPFSWNPIR
                     DPRGLGVQMMLATPAGLWVGSDTSGIGGEYHGKIAFMPLAGGASVPAPYVATLPNDLY
                     TVPQSGCLSQDPSVLYRVNAGGSLVPSGNCQIDWAVDTAAAPSPLHNTGASAVTGGTP
                     TLNSTVPAGTPIGIFSAAREDPIGGNEMQWNFNVPAGKQVKVRMYFANFAGNLKQTGR
                     RVFNVSIDGTTVLPNFDISASIGFQVGGMREFTVTSDGQVNVLFSHLVNNPIVSAIEV
                     IDPSIAPGGGGGGLEYLARRSFDGSATGTAATLSTPGTDWSHARGAFVTNGKLYSGWD
                     NGLFYSRPFDGTTVGAPTQVDLHGLTTSNFPVANITGMFFTNGRIYYTLANDARMYMK
                     YFTPEDDAVGTQTFTVSGATDGLNWSAVSGMTFAQGKIYFGRTNGNLSRMNFANGVPT
                     PGTEALVSGPATGDGKTWTSRGMFIYGQTVDGFVPTIPGKASGVSNSTSSIDLTWAAS
                     TDNVSTTLDYHVFRDGIDIATVPSASTTTVSYTDAGLVGGSQHTYRIAAVDAATNASG
                     QSPDSDPITVQTPDLTPPTVPGAPTATANGKTKVDLVWPATTDDRPGQVTYRIYRDGS
                     QIGSVTSGSNTTVSFTDTGLSPATSYTYTVDAVDVANNPSAQSEASNTVTTAGVIFAD
                     GFDGGNFTNWTSNTRVSIDGTKGATAAPSALASPTSQSAFLVKDLPAEFGSICMSTAV
                     NLTTQNGTALDLFRLRTGANGGINKVFLNTSGQLVVKSDVTNNTKNSGVALPAGWSTV
                     ELCTTVGMTGTADLYLNGTKIVNGWVQDTGTTPVAKINLGESANRTFTVNFDDVVVDA
                     FPG"
     gene            complement(5523..6518)
                     /locus_tag="VIX62_04100"
     CDS             complement(5523..6518)
                     /locus_tag="VIX62_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HEY5859444.1"
                     /translation="MKLLVIGGTQFSGRALTGMALERGDEVTIFHRGSGTDAVHPEAE
                     HVHGDRDEGVDALRGRTFDAVVDTCGYVPRAVRASTELLADSAWYGYISSISAHLEGL
                     PPGATEDSPTHQPPWPDSEEITWESYGPLKAACEEVVREGFGDRGAVIRPGYIVGPFD
                     PTDRFTSWLRRASEGGIMLAPGPADEPIQFVDARDLAAFVLHLADTRTGGTFNVVHPS
                     GTTTIGEVLEAAKTASGADTKIEWVDADWLVGRLGDDRHTQLPIWEPEDAGAHRYDSR
                     ASVAAGLETRPVADTVRDTLAWDEERRRDPEAANACGLPADRERELLEVWRSHQA"
     gene            complement(6607..>6952)
                     /locus_tag="VIX62_04105"
     CDS             complement(6607..>6952)
                     /locus_tag="VIX62_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5859445.1"
                     /translation="EASIQREVALALDRADLPDAVHCLDEVMALAVLRACAERGIRVP
                     DDLLVSSISDRGRAEVASPSLTTLELHPRELGAAAAGVLADLLAGLPPPVAPVFVETT
                     IVARASTDRRGA"
BASE COUNT         1167 a         2463 c         2299 g         1023 t
ORIGIN      
        1 gcgtggtcgc tcgtgcccgt gttcatcgcc gtggcctact cgttcaacgc ggggaaggcc
       61 cagaccacct ggcaggggtt ctcggcgacg cgctggttca tcaccgacgt caactccgtg
      121 tggcaggacg agaccttgcg ctcggcgctt ttccagagcc tgaaactgtc ggcgctcacg
      181 gtggtgatcg cggtgccgct gggcgtggcc ttcgccatcg cgctcgaccg gtggcgcggt
      241 cgcggtgcgg gcacgagcga cttcgtgatg ctgttctcgt tcatcacgcc ggagatcgcg
      301 atcggcgtgg gcctgttcct gatgatgacc aagctggtcg agcagatcgg cctggggttc
      361 acggcgcaag tcctggggtt gtcgatgtac gagctcgcct tcacggtgat catcgtgcgg
      421 gcacggttgc tgtcggtggg caagcactac gaggaagcgg ccatggacct cggcgcctcg
      481 cccaccaatg cgatccggcg ggtgctcctg ccgctgctct cgcccgcgat cttcgcgagc
      541 gtcgccatcg tgttcgcctc ggccatcgac aacttcgtga tctcccagcg cctgtgcgcc
      601 gacgcctcgt gccagacgat cccgatcgtg atctattcgt cggcgcgccg gtccccgttg
      661 ccgtcgttga acgcccttgc cacgatcgtg ctcgtgctct cgacggtgtt gatcggggcc
      721 gccgtggtcg gctaccgcgc gatgacgagg ggcgagcgcg cccttgccga gagccccggc
      781 gccgaagcgc ccgtgctagc gtccggcgga tgagcgaagg gcgtgtccgc gacctcctgc
      841 gggcgggggg cgacggcaac gccatcctgc cgggcatcga cccgcgagac acgccgggcg
      901 tggatgatcg cgagacggcc gaggcggcga accccgagct cgccgaccga ctcgaccacc
      961 tccaggaacg gctctgggtg gaggggaagc gctcgctgct ggtggtgctg cagggcatgg
     1021 acaccagcgg caagggcggc acggtccgtc acgtgttctc atcgatgaac ccggccggcg
     1081 tggacgtggc ctcgttcaag aagcccaccg acgaagaact ccaacacgat ttcctctggc
     1141 ggatcgagcg caggctcccc gctgcgggcc aggtgatggt gttcgaccgg tcccactacg
     1201 aggacgtgct gatcgtgcgg gtgcgttcac tcgtgcccga agcggtctgg cggccgcgtt
     1261 accgcgagat cgtcgagttc gaagaacggg tggcggccgc cggcaccacg atcgtgaagt
     1321 gcatgctgca catctcctac gacgagcaac gtgagcgact gctggcacgc ctcgacgacc
     1381 ccgagaagca ctggaagttc cacgagggcg acctcgagga acgacggcgc tggcaggact
     1441 accaggcggc ctacgaggaa gcgatccgcg aatgctcgac cgaggcggcc ccgtggttcg
     1501 tcgtgccctc ggaccgcaag tggtaccgga actgggccat ctcctcgctc ctggtcgaga
     1561 ccctcgagga catggatccg cagctgcccc gtcccgacct cgacgtcgat gccttgaaag
     1621 ggcaactgca accgcccaac tgagccggcc gtagcaccga aggacctagg gcatgcgtcg
     1681 cacgccatgg tgttcctgcg tctgccgagg attcatccct tcggagtaag acaccgaccc
     1741 cttgcgttcc ctatcctggg gacacccccg tatcccccga gcgaaggaac cccccgaatg
     1801 cgtcgttcca ccaccctggt catcgcgctg tcgatggccg tctccggact gagtctgctg
     1861 cccgccgcgc cggccgtcgc cgcgggcgcc cccagcaccg cgcagcccac gttggtctcc
     1921 gccgatccgg ccgactggac gcccaacatc caggacgggc aggtcaacgc gatcgtccag
     1981 gtgggcaacc aggtgatcgt cggcggtcag ttcctgcaag ccaagaacat cagcggcaac
     2041 ctgctcgacg tctcgagcat cctggcgttc aacgccacca ccgggacgat caacaagaac
     2101 ttcatgccca cgttcgacgg cgagatcctc gccctgggga ccgacggcac cagcgtctac
     2161 gtcggaggcc gtttcggcac cgtgaacggg cagaccgggt acaagcgcct ggtgaagctg
     2221 gatccggcca acggacagat cgtgacgccg ttcaaggcca agatcggcgc cggcgaggcc
     2281 gtgcacgaca tgtcgttcgc caacggcaag ctctacatcg gcggggcgtt ctcggccgtc
     2341 cagggcgtcg cccgcgagaa gttcgcggcg ctgaacccgg tgagcggcgc cctcgacccc
     2401 accgtcgacg tggcgttcac tggcaagcac aacggcggca ccggccgcat ccgcaagttc
     2461 gacatcacgc ccgacggcac caagatggtg gcggtcggca acttcaccct ggtcgacggc
     2521 caggatcgtg accaggccgc catggtcgac ctgaccacgg acacggtcgc caactggcag
     2581 accaaccgct tcaagtcggc ctgctcctcc ttcttcgaca cctacaccaa cgacatcgac
     2641 atcgcccccg acggctccta cttcgtgatc ggcaccaccg gcgccttcat gggcggcgtg
     2701 aacgccgggg tgctgtgcga cacgacgtcg cgctgggaga tcaacgccac cggtacggcc
     2761 ctgcagccca cgtgggtcga ctacaccggc ggagacacca cgttcagcgt gaccgccacc
     2821 aacacggcgg tctacgtcgg cggtcaccag cgctggtgga acaacccgta ctctgccaac
     2881 ggcgactccg cgggccaagg ggcgatcgat cgcatgggca tcgccgcgct cgacccgctg
     2941 aacggcctgc ccttcagctg gaacccgatc cgcgaccccc gtggcctggg cgtccagatg
     3001 atgctcgcca cccccgcggg cctgtgggtg ggctcggaca cctcgggcat cggcggggag
     3061 taccacggca agatcgcctt catgccgctg gcgggcggcg cttcggtgcc ggccccgtac
     3121 gtggcgacac tgcccaacga cctctacacc gtgccacaga gcggctgcct gagccaggat
     3181 ccctccgtcc tgtaccgagt gaacgccggc ggctccctgg tgccgagcgg caactgccag
     3241 atcgactggg cggtggacac cgccgcggcc ccgagcccgc tgcacaacac gggcgcgagc
     3301 gcggtgaccg gcggcacgcc gaccctcaac agcaccgtgc cggcgggcac gccgatcggc
     3361 atcttctcgg cggcccgtga ggatccgatc ggtggcaacg agatgcaatg gaacttcaac
     3421 gtgcccgcgg gcaagcaggt caaggtgcgg atgtacttcg cgaacttcgc gggcaacctg
     3481 aagcagaccg ggcggcgcgt gttcaacgtg tcgatcgacg ggacgacggt gctgccgaac
     3541 ttcgacatca gcgcctcgat cggcttccag gtcgggggga tgcgcgagtt cacggtgacg
     3601 agcgacgggc aggtcaacgt gctcttctcc cacctcgtga acaaccccat cgtgagcgcg
     3661 atcgaggtca tcgacccgag catcgcaccc ggtggcggtg gcggcggcct tgaatacctg
     3721 gcgcgtcgct ccttcgacgg ctcggcgacg gggacggcgg cgacgctgtc cacgcccggc
     3781 accgactggt cgcacgcccg tggggcgttc gtcaccaacg gcaagctcta cagcggctgg
     3841 gacaacggac tcttctactc ccgtccgttc gacggcacca cggtgggcgc gcccacccag
     3901 gtggacctgc acggactcac cacgtcgaac ttcccggtcg ccaacatcac gggcatgttc
     3961 ttcacgaacg gccgcatcta ctacacgctg gccaacgacg cccggatgta catgaagtac
     4021 ttcacgcccg aagacgacgc cgtgggcacg cagaccttca cggtcagcgg cgccaccgac
     4081 ggactcaact ggagcgccgt gagcggcatg acgttcgccc agggcaagat ctacttcggg
     4141 cgcacgaacg gcaacctctc ccgcatgaac ttcgccaacg gcgtccccac cccgggcacc
     4201 gaggcgctgg tcagcggccc ggccaccggc gacggcaaga cgtggacctc gcgcgggatg
     4261 ttcatctacg gccagaccgt ggacgggttc gtcccgacga tacccggcaa ggcgtcgggc
     4321 gtgagcaaca gcaccagctc gatcgacctg acgtgggcgg cgtccaccga caacgtcagc
     4381 acgaccttgg attaccacgt gttccgcgac gggatcgaca tcgccaccgt gccgagcgcc
     4441 tccaccacca ccgtgtccta caccgacgcc gggctggtcg gaggatcgca gcacacgtac
     4501 cgcatcgccg cggtcgatgc cgccaccaat gcgagcggtc agtcgcccga ctccgatccg
     4561 atcaccgtgc agacgcccga cctgacaccg cccacggtgc cgggggcgcc gacggccacg
     4621 gccaatggca agaccaaggt ggacctcgtg tggcccgcga ccacggacga tcgtccgggc
     4681 caggtcacgt accggatcta ccgcgacgga tcccagatcg gcagtgtgac gagcggctcg
     4741 aacaccacgg tgtccttcac cgacacgggc ctgagcccgg cgacgagcta cacctacacc
     4801 gtggacgcgg tcgacgtggc caacaacccg agcgcccaga gcgaggcgtc caacacggtg
     4861 acgaccgccg gcgtgatctt cgccgacggc ttcgacggtg ggaacttcac gaactggacc
     4921 tcgaacaccc gggtcagcat cgacggaacc aagggcgcca ccgcggcgcc gagcgcgctc
     4981 gccagcccca cgtcgcagag cgccttcctg gtcaaagacc tgccggcgga gttcgggtcg
     5041 atctgcatga gcacggccgt gaacctcacc acgcagaacg gcacggcgct ggacctgttc
     5101 cgcctgcgca ccggcgcgaa cggcgggatc aacaaggtgt tcctgaacac cagcggtcag
     5161 ctcgtggtga agtccgacgt cacgaacaac acgaagaact ccggcgtggc cctgcccgcg
     5221 ggatggagca ccgtcgagct gtgcacgacg gtcgggatga ccggcacggc cgacctgtat
     5281 ctcaacggca cgaagatcgt gaacgggtgg gtccaggaca cgggcacgac gccggtggcc
     5341 aagatcaacc tcggcgagag cgccaaccgg acgttcacgg tcaacttcga cgacgtggtg
     5401 gtcgacgcct tcccaggctg atcgagcacc gcaacgatct ccctgctcgg ggaggggctc
     5461 acgggggcgc ggccgcgccc ccgtgatcca tatctgggct cgaccaccgc cgcccggcgg
     5521 gatcaggcct gatgcgaccg ccagacctcg aggagctcac gctcgcggtc cgccgggagg
     5581 ccacaggcgt tcgcggcttc ggggtctcgc cggcgttctt catcccaggc aagggtgtcg
     5641 cgcacggtgt cggcgaccgg ccgcgtctcg agtccggccg ccaccgaggc ccgcgagtcg
     5701 taccggtggg cgccggcatc ctcgggttcc cagatcggga gctgggtgtg ccggtcgtcg
     5761 ccgagccgac cgacgagcca gtcggcgtcg acccactcga tcttcgtgtc ggcgccggat
     5821 gcggtcttcg ccgcctcgag gacctcgccg atcgtcgtcg tccccgacgg gtgcacgacg
     5881 ttgaaggtgc cgccggtgcg cgtgtcggcc aggtgcagca cgaacgccgc gaggtcacgc
     5941 gcatcgacga actggatggg ctcgtccgcg gggccgggcg ccagcatgat cccgccttcg
     6001 gatgctcgac gcagccagga ggtgaaccga tccgtggggt cgaacgggcc gacgatgtag
     6061 cccggccgga tcaccgcgcc acggtccccg aagccctcgc gtacgacctc ctcgcatgcc
     6121 gccttgaggg ggccgtaaga ctcccaggtg atctcctcgg agtcgggcca gggcggctgg
     6181 tgcgtgggcg aatcctccgt ggcgcccggc ggcagccctt cgagatgggc cgagatcgag
     6241 gagatgtagc cgtaccaggc gctgtcggcc aacagctcgg tggaggcccg gacggcccgg
     6301 ggcacgtatc cgcaggtgtc cacgacggca tcgaaggtcc gaccccggag ggcatcgacc
     6361 ccttcgtcgc gatccccgtg cacgtgctca gcctccggat ggacggcatc cgttcccgag
     6421 ccgcgatgga agatcgtcac ctcgtcccct cgttccagcg ccatgccggt caacgcccgg
     6481 cccgagaact gcgttccccc gatcaccagc agcttcatcc cgcccccttt cccgcgcact
     6541 ctacgtggac gttggagacc aggtatcgac ggtgggcgcg ggtaagaccg tctgaggcag
     6601 ctcgggctaa gcgccccgtc ggtcggtcga ggcccgcgcc acgatcgtgg tctccacgaa
     6661 gaccggggcc accggcggtg gcaggcccgc gagcagatcg gccagcacgc ccgccgccgc
     6721 cgcgccgagc tcacgagggt gcagttcgag cgtcgtgagc gacggtgacg cgacctcggc
     6781 gcggccccga tcgctgatcg agctcaccag gagatcgtcg gggacacgga tcccgcgctc
     6841 ggcgcacgcc ctgagcacgg ccagcgccat gacctcgtcg aggcagtgca cggcatcggg
     6901 cagatcggca cggtcgagcg ccagcgccac ctcgcgctgg atcgaggcct cg
//
LOCUS       DATDBZ010000167         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_263868, whole genome shotgun sequence.
ACCESSION   DATDBZ010000167 DATDBZ010000000
VERSION     DATDBZ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263868"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VIX62_04110"
     CDS             complement(<1..284)
                     /locus_tag="VIX62_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5859446.1"
                     /translation="MPDPGSRRIVVAGASGLIGSALVDSLRADGIRVTTLVRRPAEAV
                     DEVQWLTDADPLDPAVLEGADAVVGLNGATIGRFPWTRRYKSRLLWSRITP"
     gene            complement(277..702)
                     /locus_tag="VIX62_04115"
     CDS             complement(277..702)
                     /locus_tag="VIX62_04115"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5859447.1"
                     /translation="MTVTSDATTVWKGGLIDGSGQVTFESSGIGTFDLNWKARSEGST
                     SVTTPEELVAAAHSSCFSMAFSLALAQNGTPPESIDTTASVTFTPGTGITGSHLNVNA
                     VVPGLDREDFDRIAADAKVNCPVSQALAGIEITLEATLA"
     gene            complement(785..1297)
                     /locus_tag="VIX62_04120"
     CDS             complement(785..1297)
                     /locus_tag="VIX62_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEY5859448.1"
                     /translation="MSDRTDTASKPRGIDPRGPRFAAGITALLLLVDVFLGLTTPIGA
                     TVAERAAEPAFLLLVVIALLFLWGVLSPRTAPWGALYRTLIQPRLTPPTDLEDPRPPR
                     FAQGVGLIVVTIGLILHLAGVPWALPIAAAAAFVAAFLNAVFGLCLGCQLYLVLQRAG
                     IVGRERPATA"
     gene            complement(1290..1727)
                     /locus_tag="VIX62_04125"
     CDS             complement(1290..1727)
                     /locus_tag="VIX62_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5859449.1"
                     /translation="MTLVNALIGLAVLLAATVALGVFVQWRQGRPRRHIPHEVVQPTR
                     LGAESLGATATLLQFSTELCSRCPGVHRTLAAVADARAGVEHLDIDVTHRPDIAKHFH
                     ILQTPTTLVLDRDGVVQTRFGGVPNREVLELELGRLTGETANV"
     gene            1852..>2069
                     /locus_tag="VIX62_04130"
     CDS             1852..>2069
                     /locus_tag="VIX62_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY5859450.1"
                     /translation="MSETIPTPYEDLLRDVLEHGAVKTDRTGTGTRSVFGRQLRFDLS
                     QGFPLVTTKRVHMKSIAYELLWFLRGES"
BASE COUNT          337 a          670 c          762 g          300 t
ORIGIN      
        1 ggcgtgatcc gcgaccacag cagtctgctc ttatagcgcc gggtccaggg gaatcgcccg
       61 atcgtcgctc cgttgagccc gacgaccgcg tccgctcctt cgagcacggc ggggtccagg
      121 ggatcggcat ccgtgagcca ctggacctcg tcgaccgcct cggcaggtcg acgcaccagc
      181 gtggtgaccc ggatcccgtc ggcgcgcaga ctgtcgacca gagccgagcc gatcaggccc
      241 gacgcgccgg cgacgacgat ccgacgggag ccgggatcag gcaagcgtgg cctcgagggt
      301 gatctcgatg ccggcgaggg cctgcgagac cgggcagttg accttcgcgt ctgccgcgat
      361 ccggtcgaaa tcctcgcgat ccaggccggg caccaccgcg ttgacgttga ggtggcttcc
      421 ggtgatgccg gtgccgggcg tgaacgtgac ggaggcggtc gtgtcgatcg actcgggcgg
      481 ggttccgttc tgggccaggg cgagcgagaa cgccatgctg aagcacgacg agtgggctgc
      541 cgcgacgagc tcttccggcg tggtcaccga ggtcgagcct tcgctgcggg ccttccagtt
      601 gaggtcgaag gtgccgatcc ccgaggactc gaacgtgacc tggcccgatc cgtcgatgag
      661 gccgcccttc cagacggtcg tggcatcact cgtgacggtc atcggaacct cctggtcgac
      721 gtgcccccgt gctccgggcg cctcgccgtc agcctaaccg cggcacaccc tcggcgcgac
      781 cgattcaggc cgtggcgggt cgctcgcgac cgacgatgcc ggcgcgctgc agaaccaggt
      841 agagctgaca gcccaggcac aggccgaaca cggcgttgag gaacgcggcg acgaaggcgg
      901 ccgcggccgc gatcgggagt gcccacggga cccccgcaag gtgcaggatc aggccgatcg
      961 tcacgacgat gagaccgacg ccctgcgcga agcgtggcgg gcgcgggtct tcgaggtcgg
     1021 tcgggggcgt gagccggggc tggatcagcg tgcggtagag cgctccccat ggggcggtcc
     1081 gaggcgaaag gacgccccac aggaacagca gcgcgatcac gaccagcagc aggaacgccg
     1141 gctcggcggc gcgctcggcg accgtcgcgc cgatcggcgt ggtgagaccg aggaagacgt
     1201 cgaccagcag aagcagggcg gtgatgccgg cggcgaagcg cggaccacgg gggtcgatgc
     1261 cacgcggctt ggaggcggta tccgttcgat cagacattcg cggtctctcc ggtcaggcgg
     1321 ccgagttcga gttcgagcac ttcgcggttg ggaaccccgc cgaagcgcgt ctgcacgacg
     1381 ccgtcacggt cgagcacgag cgtggtcggg gtctgcagga tgtggaagtg cttggcgatg
     1441 tcgggacggt gcgtcacgtc gatgtcgagg tgctccacgc ccgcgcgagc gtcggcgacg
     1501 gcagcgaggg tccggtgcac tccgggacag cgcgagcaga gctcggtgct gaactgcagg
     1561 agggtggccg tcgcaccgag cgattcggcg cccagacggg tcggctgcac cacttcgtgg
     1621 gggatgtgcc ggcgcggtct gccctgccgc cactgcacga agacgccgag ggcaaccgtc
     1681 gctgccagca ggacggcgag gccgatgagc gcgttgacga gggtcacagc atcccaggct
     1741 aactgcttgc ggctgaatac gtgtcggatg cgggttgtgt gacgagccga tcgtcacaaa
     1801 caccccgtcg cctcggtccg tcgtcggtgg gggagggata ccatcgagac gatgtccgag
     1861 acgatcccga ctccttacga agacctgctc cgcgacgtgc tcgagcacgg cgccgtcaag
     1921 accgatcgca ccggaacggg cacgcgcagc gtgttcggcc ggcagctgcg cttcgacctc
     1981 tcgcagggct ttccgctggt cacgaccaag cgcgtgcaca tgaagtcgat cgcgtacgag
     2041 ctgctctggt tcctgcgcgg cgagtccaa
//
LOCUS       DATDBZ010000168         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_670106, whole genome shotgun sequence.
ACCESSION   DATDBZ010000168 DATDBZ010000000
VERSION     DATDBZ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670106"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VIX62_04135"
     CDS             <1..231
                     /locus_tag="VIX62_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY5859451.1"
                     /translation="VIDGVVNGAATLARGLGQVVAWFDRTVIDGIVNGAGETAGRTGN
                     VLKYLQTGTVQWYAVGLFVGVIAMAVVFIRVV"
     gene            251..1786
                     /locus_tag="VIX62_04140"
     CDS             251..1786
                     /locus_tag="VIX62_04140"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5859452.1"
                     /translation="MTWENSAITITTLLPIVGALVIMVMPRDKDRIVRGLGIVFTGAA
                     LVLATAIAIGFDYGTDGLQYVLDVDWIASIGVRYHVGIDGISLPLYVLTFLLSFLCAI
                     YSWRYVPDPGRTKAFLALMLLLETGMAGTFIAFDLILFFVFWEMVLVPMYFLIGIWGS
                     ANREYASIKFFLYTLFGSIFMLLGFLAMYFQGPDPHTFDMIVLRDYGLAGGFTQGFQL
                     VVFGAVALGFAVKVPMWPFHTWLPDAHTEAPTIGSVLLAGVMLKMGTYGFIRIAIPFL
                     PYGAEKYAPWIGALAAIGIVYAALACLAQKDLKRLIAFSSVGHMGFVMLGIATLTEIG
                     IQAAIFGMVAHGIITGMLFFCVGSIYDRYHTREIAKIGGGMLQAMPKLGSVFAFVAIA
                     SLGLPGLAGFWGEVMALLSSFSPNEVFLADQVGLFRVFMVMGGIGTVLTAGYFLWMLQ
                     RVNMGTVPDQWREAGLRDVLVVEWVSWVPLLLLTLVLGLYPRLVFGVTDEAVGGLMKL
                     LGS"
     gene            1796..>2560
                     /locus_tag="VIX62_04145"
     CDS             1796..>2560
                     /locus_tag="VIX62_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5859453.1"
                     /translation="MDVDYHALLPEIILASTILIVLVVDVFLPERTKWLAMPIGLVGV
                     AGSFVAVLTLIGADTRISFGGSYVIDEFALLFKGFFLVVASVVLALSLRYFREGGFYQ
                     GEYYFLLLTSFLGCLMMPSSRDLLLLFIALELVSAPGFLMAAFRKGDVKSNEAGVKFF
                     IIGVLSTAVMLFGMSLIYGVTGGQTQLAEIAVALDGVTGDLETLAFAAILFVVVGFAF
                     KVSAFPFQFWAPDTYEGSPVPVAAFLAVASSVAGFAG"
BASE COUNT          333 a          813 c          882 g          532 t
ORIGIN      
        1 gtgatcgacg gcgtcgtgaa cggggccgcg acgctcgcga gagggctcgg ccaggtggtc
       61 gcctggttcg accgcaccgt gatcgacggc atcgtcaacg gtgccggcga gaccgccggg
      121 cgcaccggca acgtgctgaa gtacctgcag acgggcaccg tgcagtggta cgccgtcggg
      181 ctgttcgtcg gcgtgatcgc gatggccgtc gtgttcatcc gagtcgtctg agaggaagcg
      241 tggaggccac atgacctggg agaactcggc catcacgatc accacgctgc tgcccatcgt
      301 gggcgcgctg gtgatcatgg tgatgccgcg ggacaaggac cgcatcgtgc gtggcctcgg
      361 catcgtgttc acgggggcgg cgctcgtgct ggccaccgcg atcgcgatcg ggttcgacta
      421 cggcaccgac ggcctgcagt acgtgctcga cgtggactgg atcgcgtcga tcggggtgcg
      481 ctaccacgtc ggcatcgatg gcatctccct accgctgtac gtgctgacgt tcctgctgtc
      541 gttcctctgc gcgatctaca gctggcgtta cgtgcccgac cccggccgta cgaaggcgtt
      601 cctcgccctc atgctgctgc tcgagaccgg catggcgggc acgttcatcg cgttcgacct
      661 gatcctgttc ttcgtgttct gggagatggt gctggtgccg atgtacttcc tgatcggcat
      721 ctggggctcg gccaaccgcg agtacgcgtc gataaagttc ttcctctaca cgctgttcgg
      781 atcgatcttc atgctgctgg gcttcctcgc gatgtacttc caggggcccg acccgcacac
      841 cttcgacatg atcgtgctgc gcgactacgg cctcgccggc ggcttcacgc aggggttcca
      901 gctggtggtg ttcggggcgg tggcgctcgg gttcgccgtg aaggtgccga tgtggccgtt
      961 ccacacgtgg ctgcccgacg cccacaccga ggccccgacg atcggctcag tgctgctggc
     1021 gggcgtcatg ctgaagatgg gcacatacgg gttcatccgc atcgcgatcc cgttcctgcc
     1081 gtacggggcc gagaaatacg caccgtggat cggcgcgctg gccgcgatcg gcatcgtgta
     1141 cgcggcgctc gcctgtctcg cgcagaagga cctgaaacgc ttgatcgcgt tctcgtcggt
     1201 cgggcacatg ggcttcgtga tgctcggcat cgcgacgctc accgagatcg gcatccaggc
     1261 cgccatcttc ggcatggtgg cccacggcat catcaccggc atgctgttct tctgcgtcgg
     1321 gtcgatctac gaccgctacc acacgcgcga gatcgcgaag atcgggggcg gcatgctgca
     1381 ggcgatgccg aagctcggca gcgtcttcgc gttcgtggcg atcgcctcgc tggggttgcc
     1441 cggcctcgcg gggttctggg gcgaggtgat ggccctgctc agctcgttca gcccgaacga
     1501 ggtcttcctg gcagaccagg ttggtctgtt ccgtgtgttc atggtgatgg gcggcatcgg
     1561 caccgtgctc acggccggct acttcctgtg gatgctgcag cgcgtgaaca tgggcaccgt
     1621 gcccgaccag tggcgcgagg cgggcctgcg cgacgtgctc gtggtcgagt gggtgtcgtg
     1681 ggtgcccctg ctgctgctga ccctggtgct cgggctgtac ccgcgcctcg tgttcggtgt
     1741 caccgacgag gccgtgggcg gtctcatgaa gctgctgggg agctaggtca cctcgatgga
     1801 cgtcgactac cacgccctac tgcccgagat catcctcgcg agcacgatcc tcatcgtgct
     1861 ggtcgtcgac gtgttcctgc ccgagcgcac gaagtggctc gcgatgccga tcggactcgt
     1921 cggggtggcg ggctcgttcg tcgcggtgct gacgctcatc ggcgccgaca cgcgcatcag
     1981 cttcggtggc tcctacgtga tcgacgagtt cgccctgctg ttcaaggggt tcttcctcgt
     2041 ggtggcgagc gtggtgctgg ccctgtcgct gcggtacttc cgcgaaggcg gcttctacca
     2101 gggcgagtac tacttcctgc tgctcacgtc gttcctcggt tgcctgatga tgccgagctc
     2161 gcgcgacctg ctcctgctgt tcatcgcgct cgagctcgtg tcggcgcccg ggttcctgat
     2221 ggccgcgttc cgcaagggcg acgtgaagtc gaacgaggcg ggcgtgaagt tcttcatcat
     2281 cggcgtgttg tcgacggccg tgatgctctt cggcatgagc ctgatctacg gcgtgacggg
     2341 cggtcagacg cagctcgccg agatcgcggt cgccctcgac ggcgtgacgg gcgacctcga
     2401 gacgctggcg ttcgcggcca tcctgttcgt ggtggtgggc ttcgcgttca aggtctcggc
     2461 gttcccgttc caattctggg cgcccgacac gtacgaaggt tctccggtgc cggtcgcggc
     2521 cttcctcgcg gtcgcctcga gcgtggcggg tttcgcgggc
//
LOCUS       DATDBZ010000169         3587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_670585, whole genome shotgun sequence.
ACCESSION   DATDBZ010000169 DATDBZ010000000
VERSION     DATDBZ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670585"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VIX62_04150"
     CDS             complement(<1..908)
                     /locus_tag="VIX62_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5859454.1"
                     /translation="MPDAARPSTAKIWTILGALYFVWGTTYLGIEKVNETVPTLLGAA
                     ARFLFAGTVLFAWTSMRTHERTTPKQWRAAGIVGIFLLLGGNALVAVSENMGTPTSIV
                     SLIIALIPLWLAFFDRVVFRAAPLGWKVVVGLVGGFTGAALLVSGSAGDGVPVGGMLV
                     AVAATLCWTVGSLYARTAPLPKAPLLGSGMQQLVGGGVILLVALVVGEAADFNLSAIS
                     SSSWLGLAWLIVAGSFVGFSAYLWLLRNVRTSLVSTYAYVNPIVAVTLGWFVLEEDLT
                     ARLFISGAIVLASVALIVSSGAAARRT"
     gene            complement(936..1382)
                     /locus_tag="VIX62_04155"
     CDS             complement(936..1382)
                     /locus_tag="VIX62_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859455.1"
                     /translation="MTEGSGAKDASRTFDDQDRARGQDGLGDLGQEERRELLAWVGQN
                     LTEVRQTALGQRPVYWILWLGLAVGLVAYAAGYLIKASTPTEPLGLVGDLLYTFGWAL
                     WTGVVVVVFLQIIPETKRRQYKRALDAYEASLREAAAAGSEEASAE"
     gene            1492..1935
                     /locus_tag="VIX62_04160"
     CDS             1492..1935
                     /locus_tag="VIX62_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859456.1"
                     /translation="MKVEQLYRIRFTYPESWKVELEGGWEQHLYLAEGRCEGSISGRF
                     RGVNFPRRQGVDGPFRPDLRAVIETDDGAVIMFEWHGYGRAYPKGHRQIVGAVFHLSD
                     AEQYRRLNDAVCVCVGEVRAPADPDQAEPDLVIDVAELIWEPIAD"
     gene            2000..3454
                     /gene="gatB"
                     /locus_tag="VIX62_04165"
     CDS             2000..3454
                     /gene="gatB"
                     /locus_tag="VIX62_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY5859457.1"
                     /translation="MTEVEAVIGLECHVELDTQTKMFCGCRAEFGAPPNTNVCPVCLG
                     HPGSLPVPNREAIRRIILIGLAVGSEIAPHSLFHRKNYFYPDMPKNYQISQYDLPICV
                     GGHLDVDLPDGTTSRIGITRVHMEEDTGKTVHASATGRIHDADAALIDFNRAGVPLVE
                     CVSEPDIRSPEEAAAYLRELRATLESLDVSDVRMEEGSLRCDANISMRPVGTEAFGTK
                     VEIKNMNSIRSLERALHFEIERQTKALEAGEAIAQETRHWDEDSGSTKSMRSKEEAFD
                     YRYFPEPDIPALEPSEEWIAEIRAGLPELPRARRARYETDDRLKPDVARVLVGDRASS
                     ALFDETVALGADPKAAANWITQDLAGSLNKLGVDPAASPVTAQHIADLIALVADDTVS
                     GAGAKQALEEAVATGDPIAEIVERKGLTQVSDSGALGAIADEVLAENPEVVEQFRGGK
                     EAVIGFLVGQVMKKSAGSANPKLAKELLLERLSG"
BASE COUNT          653 a         1228 c         1194 g          512 t
ORIGIN      
        1 gtccggcgag ccgcagcacc cgaggacacg atcaaggcca cggaagccag cacgatggca
       61 ccgctgatga acagccgggc cgtcaggtcc tcctccagca cgaaccaccc caaggtcacg
      121 gcgacgatcg ggttcacgta cgcgtaggtg gagacgagcg acgtgcgcac gttccgcagc
      181 agccacaggt aggcgctgaa cccgacgaag gatccggcga cgatcagcca ggcgagaccg
      241 agccaggacg acgatgagat cgcgctcagg ttgaagtcgg cggcctcccc gacgacgagg
      301 gcgacgagca ggatgacccc gccgcccacc agctgctgca tgccgctgcc gagcagggga
      361 gccttcggca gcggagccgt gcgcgcgtag agggatccga cggtccagca cagcgtggcg
      421 gcgaccgcga cgagcatgcc gccgaccggc acgccgtcgc cggcactgcc gctcaccagc
      481 agcgcggcgc cggtgaaccc tccgacgagc cccacgacca ccttccaccc gagcggtgcc
      541 gcacggaaca cgacccgatc gaagaacgcg agccacagtg ggatcagcgc gatgatcaac
      601 gacacgatgc tcgtgggggt gcccatgttc tccgacaccg ccacgagcgc gttcccgccg
      661 agcagcagga agatgcccac gatgccggcg gcgcgccact gcttcggggt cgtgcgctcg
      721 tgcgtgcgca tcgaggtcca cgcgaacagg accgtgcccg cgaacaggaa ccgcgccgca
      781 gcgccgagca aggtgggcac cgtctcgttg accttctcga tgcccagata ggtcgtgccc
      841 cagacgaaat agagggcgcc caggatcgtc cagatcttgg cggtggacgg gcgggcggca
      901 tcgggcatcg agccaggata gccggggcta ggcggtcact cggccgaggc ttcctcgctt
      961 ccggctgcgg ctgcttcgcg gagcgatgcc tcataggcat cgagcgccct cttgtactgg
     1021 cgccgcttcg tctccgggat gatctggagg aacaccacca cgacgacacc ggtccacagt
     1081 gcccaaccga acgtatacag caggtcgcca accagcccaa gaggctccgt tggcgtcgag
     1141 gctttgatca ggtacccagc ggcatacgcc accaagccga ccgccaagcc gagccagagg
     1201 atccagtaga cgggtcgctg accgagcgcg gtctgccgca cctccgtcag gttctggccc
     1261 acccacgcca ggagttctcg gcgttcctcc tgcccgagat cacccaaccc gtcctgccct
     1321 cgagcgcggt cctggtcatc gaacgtgcgt gaagcatcct tcgctcctga tccttccgtc
     1381 atggtcgaac tcctctcgat cgacagactc agggcgatcc acgccagcaa tcgaaccacg
     1441 gaagacctga gatagcgttg tccggatgtc ggcgccggaa caggaggaca ggtgaaggtc
     1501 gagcagctgt atcggatccg gttcacgtat cccgagagct ggaaggtcga gctcgagggc
     1561 ggctgggaac agcacctgta cctcgccgag ggtcgttgtg agggatcgat cagcggccgg
     1621 ttccgaggcg tgaattttcc ccgccgccag ggggtcgatg gcccgttccg gcctgacctc
     1681 cgcgccgtga tcgagaccga cgacggggcc gtgatcatgt tcgagtggca tgggtacggg
     1741 cgtgcctacc ccaagggaca ccggcagatc gtcggcgcgg tcttccatct cagcgacgct
     1801 gagcagtatc ggcgcctcaa cgatgccgtg tgcgtctgtg tcggcgaggt gcgcgcgccg
     1861 gccgaccccg atcaagcgga gcccgacctc gtgatcgacg ttgccgagct gatctgggag
     1921 ccgatcgccg actgacgccc cggaccggga cgctgacgaa gaggcgaccg ttggggcggg
     1981 ccgttaggct aggcgcctca tgaccgaggt cgaagccgtc atcgggctcg aatgccatgt
     2041 cgagctcgat acccagacga agatgttctg cggctgccgc gctgagttcg gcgcgccgcc
     2101 caacacgaac gtgtgtccgg tctgcctggg ccacccgggc tcgttgccgg tgcccaaccg
     2161 cgaggcgatc cgccgcatca tcctgatcgg cctggccgtc ggcagcgaga tcgcgccgca
     2221 ctcactgttc caccggaaga attacttcta cccggacatg ccgaagaact accagatcag
     2281 ccagtacgac ctgccgatct gcgtgggcgg acacctcgac gtcgacctgc ccgacggcac
     2341 caccagccgc atcggcatca cgcgggtcca catggaagag gacaccggca agaccgtgca
     2401 cgcgagcgcc acgggccgca tccacgacgc cgacgccgcc ctgatcgact tcaaccgtgc
     2461 gggcgtgccc ctggtcgagt gcgtgagtga gcccgacatc cgttctccgg aggaggcagc
     2521 cgcgtacctc cgtgaactcc gcgccacgtt ggaatcgctc gacgtcagcg acgtgcgcat
     2581 ggaggaaggt tcgctgcgct gtgacgccaa catctcgatg cgaccggtcg gtaccgaggc
     2641 cttcggcacc aaggtcgaga tcaagaacat gaactcgatc cgctccctgg agcgcgcgct
     2701 ccatttcgag atcgagcggc agaccaaggc gctcgaggcc ggcgaggcga tcgcgcagga
     2761 gacgcgtcac tgggacgagg actcgggctc cacgaagtcg atgcgttcga aggaggaagc
     2821 gttcgactat cgctacttcc ccgaacccga catcccggcg ctggagccca gcgaggagtg
     2881 gatcgccgag atccgagccg gcctgcctga actgccgcgc gcccgccgtg cccgctacga
     2941 gaccgacgac aggctcaagc ccgacgtggc tcgcgtgctg gtcggggacc gcgcgtcgtc
     3001 tgccttgttc gacgagaccg tggccctggg ggctgatccc aaggcggccg cgaactggat
     3061 cacccaggac ctggcgggtt cgctcaacaa gctcggcgtc gatcccgccg cctcgccggt
     3121 cacggcgcag cacatcgccg acctgatcgc gctggtcgcc gacgacacgg tcagcggggc
     3181 cggcgcgaag caggcgctcg aagaagcggt ggccaccggc gatcccatcg ccgagatcgt
     3241 cgagcgaaag ggtctcaccc aggtctccga cagcggcgcg ctcggggcga tcgccgacga
     3301 ggtcctggcc gagaaccccg aggtcgtcga acagttccgc gggggcaagg aggccgtgat
     3361 cgggttcctg gtgggccagg tcatgaagaa gtcggcgggc tcggccaacc cgaagctcgc
     3421 gaaggagctc ctgctcgaac ggctctccgg gtaggaacgc ggaaccggtg cacaccccac
     3481 cggagcgtcc gcagcgggtg cctttctggc agctggtgct gtacggcgcc tcgagcatgg
     3541 tccgcggcgc gatccatccc ttccagcgga tgtggcgctc caccgat
//
LOCUS       DATDBZ010000170         8448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2719040, whole genome shotgun sequence.
ACCESSION   DATDBZ010000170 DATDBZ010000000
VERSION     DATDBZ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719040"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VIX62_04170"
     CDS             complement(<1..190)
                     /locus_tag="VIX62_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY5859458.1"
                     /translation="MTWLLGVLIFFPFLTVIIAIHEAGHFFAARHYGMKVTEYFVGFG
                     PWKLWSRRKGELEVGVKAI"
     gene            complement(206..1363)
                     /gene="dxr"
                     /locus_tag="VIX62_04175"
     CDS             complement(206..1363)
                     /gene="dxr"
                     /locus_tag="VIX62_04175"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY5859459.1"
                     /translation="MKSVTILGSTGSIGTQALDVVRRNPDRFKVVGLSAAGANQELLV
                     GQVREFLPPHVAIADEQAAADVKAKIGALPGVEVIVGPDAAERLAGETETDLVLNGLV
                     GSAGLAPTLATLQSGKTLALANKESLVVGGELVTDLIKGEPERLLPVDSEHAALAMAL
                     RGERREDLKRVVLTGSGGPFRGWTRSELARASVKEALQHPVWSMGPKITIDSATLMNK
                     GLEVIEAHYLFDLEYSSIHVLIHPEGLVHAMAEFRDGSLRAEMAQPDMRLPIQLAMTW
                     PERLSTGVEPVPLTEKALTFEPVDREAFPAVDLAYRVGGLGLTFPAVMNAANEVAVMA
                     FLEGKIPLNRIVELVQTVVDEHEPASVVSVVNIERADTWARQRTAEIIEDR"
     gene            complement(1439..3178)
                     /locus_tag="VIX62_04180"
     CDS             complement(1439..3178)
                     /locus_tag="VIX62_04180"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY5859460.1"
                     /translation="MADDKRDGDDLFEDLDKFFAPIKDVDWDEPASAGARETPSEEHV
                     AVRAPDPAPLATGSRTPEPSEDEPTVLDEPAPVSASAEDDDDEEWYDTTVLDTIEGIG
                     TDEPDEAPQLETTTEVVSLDDLQVSDAPIGETAVDQAGLFASDDPADAEPGDGWSLVE
                     DDEQPDVSGIIGTDTQPMSTIEEEVVVTAAPSDEELEAAADHFAPTADEPASALPEEE
                     VIVGGVVGFDDDETPESYAGPAVGGDLLGELGADEVEQDILSDLQPEDEPQTVMVGAG
                     GEGLGGPSWQEPTSVEVGADAERRSSPGGDRDVPAAFMTGAILAAIALVSLLIGKGVF
                     GVIAGAVVLLAQGELYGVMVKHHRQPATAVGLVTGALMLGGAYFKGEGAVLAMFAMGV
                     VATFLWFMSSPAATRKDTAVNIGLTILNLAWIPLLGSYLFIMLDAPNGTDLVVAVIAL
                     TFVYDTAAFLSGSVWGGQFFPRPLAPSVSPKKSIEGVIVGTLVTILVSVTLVSAFVDV
                     FQDMRIETLLLGLVVAAAATFGDLAESLLKRDVGIKDMSGMLPGHGGVLDRIDSLLFV
                     APATFLFFRIIFT"
     gene            complement(3178..3738)
                     /gene="frr"
                     /locus_tag="VIX62_04185"
     CDS             complement(3178..3738)
                     /gene="frr"
                     /locus_tag="VIX62_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY5859461.1"
                     /translation="MTIDSALKDADHKMEQAVSHLKEDLAGIRTGRASASVLNRVTVE
                     YYGTPVPLNQLASVTVPEPRLLAVTPFDKGAIGAIEKAIQSSDLGITPSNDGQVIRLA
                     FPPLTEERRKDLVKQLHHRSEEGRVAVRNVRRHSKEEMEKLEREGGISQDDLARGEKE
                     LQKVTDRFVAEIDEIQGHKEQELMEV"
     gene            complement(3750..4478)
                     /gene="pyrH"
                     /locus_tag="VIX62_04190"
     CDS             complement(3750..4478)
                     /gene="pyrH"
                     /locus_tag="VIX62_04190"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY5859462.1"
                     /translation="MADQQHGTRPQYNRVLLKLSGEAFAPADAESGINTDATALVARQ
                     LAEVVDLGVQTAVVVGGGNIWRGRQAPGIERNRADYMGMLATVMNALALQDALEKIHV
                     TTRVQTAISMSQLAEPFTPLRARRHLEKGRVVIFAAGLGVPFFSTDTCAAQRALEIGA
                     EALLKGTKVDGVYTADPHLDASATRFDAIEYVEVLKEGYQVMDATAISLCMENKLPII
                     VFDLGEEGNIRRVVGGEPIGTLVH"
     gene            complement(4517..5125)
                     /gene="tsf"
                     /locus_tag="VIX62_04195"
     CDS             complement(4517..5125)
                     /gene="tsf"
                     /locus_tag="VIX62_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY5859463.1"
                     /translation="MSDISAAQVKELRDRTGAGMMDCKKALVETGGDMEKAVELLRQK
                     GLASAAKRAGRSANQGLIEAYIHFNNTVGVLVEVNCETDFVANTDEFKALAKDIALHI
                     ASPAAPRFVSREEVSQDEIDHESQIFAKQAQESGKPENVVDKIVEGKINSYFKEIVLL
                     DQPFVKDDSKTVQQLLDETSARVGEKVAVKRFVRYKLGEDTE"
     gene            complement(5135..6133)
                     /gene="rpsB"
                     /locus_tag="VIX62_04200"
     CDS             complement(5135..6133)
                     /gene="rpsB"
                     /locus_tag="VIX62_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY5859464.1"
                     /translation="MPVVTRRELLEAGVHFGHQTRRWNPKMHRYLYGERSGIYIIDLE
                     KSLSGLEETYEFVRNLGRRRGIVLFVGTKKQAQEVVQEQSARVGMPYVNHRWLGGMLT
                     NFTTVSKRLLRLRELREMDRTGALDYLPKKEAIRLRHERDKLQRNLGGIQDLERLPDA
                     IFVIDTKKEHIAVNEARKLNIPVIAIVDTNCDPDEVDFVIPGNDDAIRAVNLVTRVVA
                     DALAAGYGMAKDEAVERVTGAAPKATGPKATAAPARVEEHETPSAEDAAAIAASTVFE
                     PDAPSDAETAAAAAEAAEEPGAPVEAEAGAPAATTEAPEAEVAPTPEPVAADTTQE"
     gene            complement(6298..7251)
                     /locus_tag="VIX62_04205"
     CDS             complement(6298..7251)
                     /locus_tag="VIX62_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859465.1"
                     /translation="MTNKNKKRPSGYRESPSASTDVAQPPRRRGLFDSILAPRVPGST
                     PMPSILTSLGRGCVTVASSIPLVLTSILLLLAMWLVLVGLGYEGPFALLVNVLGVPPV
                     GTFNDLNLSISVFGVTTGFLALLVFIGLRAIIQAVLTVMVVDVLQTGSATRWSVVRSL
                     RILPVTLAVNVACLGLLMASSFVGPLLGQGFGLLILLASLVAGVYLLGFAPAIAATER
                     RGLADTLGRSVRAGRLPGAGNLMFAAIYVVTFVAVLAIPGKPGAMVGVNPSIAAWVVG
                     IVVGLAHVAVLAALVFRYLSIADVVPEAAPRRQPAARGRRR"
     gene            complement(7248..>8448)
                     /locus_tag="VIX62_04210"
     CDS             complement(7248..>8448)
                     /locus_tag="VIX62_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5859466.1"
                     /translation="VVYTEGFGDGPPDAAGSASPSASASGSASPSSSDGATQEERFDS
                     HLAAFDLDTQEPLWDPVALSAVSRTGVTVGDGMAYLGVNGGTIYAVDLAKGKVAWTAE
                     LGRPVATQPAFTAGTVVVGLQASRQEPHPTVVALDASSGEERWRADDESQAAIVSTAS
                     IAGGTVYVAFTGGQESSIDAFDLDTGGRRWRTRFPRFFDLTATTPPVVTDDAVYVTDA
                     QGETYRLDPATGTRAWEFALNEGVLRTAPIAVGDHVLVGTIDGSLVALEASGGDLVWR
                     DDGDGSPLRALAVAGDRVIAVRGGAGAGLVAFVHDDGVALVREVSPTTLNLGLLLGAF
                     AVAALVVAGIVLLAGRVLVGRIGPAFPNDEDPLEGDLDDDDEEDDEEDEDDDGDPAQD
                     DDDEDDR"
BASE COUNT         1260 a         2902 c         2914 g         1372 t
ORIGIN      
        1 agatcgcctt gacgccgact tcgagctcac ccttgcggcg ggaccacagc ttccacggac
       61 cgaaccccac gaagtactcg gtgaccttca tcccgtagtg gcgcgcggcg aagaagtgcc
      121 ccgcctcgtg gatcgcgatg atgaccgtga ggaacgggaa gaagatgagg acgccgagga
      181 gccaggtcac gggggaagtc cttcgctagc gatcctcgat gatctctgcc gtccgttggc
      241 gcgcccacgt gtcggcgcgc tcgatgttca cgacggagac caccgacgcc ggttcatgtt
      301 cgtcgaccac ggtctgcacc agctcgacga tgcggttcag cgggatcttg ccctcgagga
      361 aggccatgac ggccacctcg ttcgcggcgt tcatcaccgc cgggaaggtg agccccagtc
      421 ctccgacgcg gtaggcgagg tcgaccgcgg ggaacgcctc gcgatcgacc ggctcgaacg
      481 tgagcgcctt ctcggtgagc ggcaccggtt ccaccccggt ggacagtcgc tcaggccagg
      541 tcatggcgag ctggatcggc agccgcatat cgggctgggc catctccgca cgcaagctgc
      601 cgtcgcggaa ctcggccatg gcgtgcacga ggccctcggg gtggatcagc acgtggatcg
      661 aggagtactc caggtcgaag aggtagtgcg cttcgatcac ctcgagcccc ttgttcatca
      721 aggtggcgga gtcgatcgtg atcttcgggc ccatcgacca cacggggtgc tgcagggctt
      781 ccttcacgct ggcgcgcgcg agctcgctgc gggtccagcc tcggaacggt cccccggacc
      841 cggtgagcac gacgcgcttg aggtcctccc tgcgctcgcc gcgcagcgcc atcgccagcg
      901 cggcgtgctc ggaatcgacc ggcagcaggc gttcgggttc gcccttgatc aggtcggtga
      961 cgagctcccc gccgacgacg agcgactcct tgttggccag cgccagggtc ttgcccgact
     1021 gcagggtggc cagcgtgggc gccagccccg ccgacccgac gagtccgttc agcaccaggt
     1081 cggtctcggt ctcgccggcg agccgctcgg cggcgtcggg cccgacgatc acctcgacgc
     1141 ccgggagggc accgatcttg gccttcacgt cggcggccgc ctgctcatcc gcgatcgcga
     1201 cgtgcggcgg caggaactcc cgcacctgac ccaccagcag ctcctgattg gcgcccgccg
     1261 cggacagccc cacgaccttg aagcggtcgg ggttgcggcg cacgacatcg agcgcctggg
     1321 tgccgatcga gccggtggag cccaggatgg tcacggactt catgcgagca agcctacccc
     1381 gaggccgcac gaacggcggg attccgggct gttcggccca cgcggggcaa ccgccgactc
     1441 aggtgaagat gatccggaag aacaggaacg tggccggggc cacgaacagc aaggagtcga
     1501 tgcggtcgag caccccgccg tgacccggca gcatgccact catgtccttg atgcccacgt
     1561 cccgcttcag gagcgactcc gcgaggtcgc cgaacgtcgc cgcggccgcc accaccaggc
     1621 cgaggagcag cgtttcgatc cgcatgtcct ggaacacgtc cacgaacgcc gacaccaggg
     1681 tgaccgacac gaggatcgtg accagggtgc cgacgatcac gccttcgatc gacttcttcg
     1741 ggctgacgct gggcgccagc gggcgcggga agaactgtcc gccccacacc gacccggaca
     1801 ggaacgcggc cgtgtcgtag acgaacgtca gggcgatgac ggccacgacg aggtcggtgc
     1861 cgttcggcgc gtccaacatg atgaacagat agctgcccag cagcgggatc cacgccaggt
     1921 tcaggatcgt gagcccgatg ttgaccgcgg tgtccttccg ggtcgccgcc gggctggaca
     1981 tgaaccacag gaaggtggcc acgacgccca tcgcgaacat cgcgagcacg gcgccctcgc
     2041 ccttgaagta ggcgccgccg agcatcagcg cacccgtcac cagccccaca gcggtggccg
     2101 gctgacggtg gtgcttcacc atcacgccgt acagctcgcc ctgggccagc agcaccacgg
     2161 cgccggcgat gacaccgaac acgcccttgc cgatcagcaa cgacaccaac gcgatggcgg
     2221 cgaggatcgc gccggtcatg aacgcggcgg gaacgtcacg atcgcctccg ggggaactcc
     2281 tgcgctcggc atcggcgccc acttcgacgg aggtgggctc ctgccagctg gggccgccga
     2341 ggccttcgcc gccggcaccc accatcacgg tctggggttc gtcttcgggc tggaggtcgg
     2401 acaggatgtc ctgctcgacc tcgtcggcgc cgagttcacc caggagatca ccaccgaccg
     2461 cgggtcccgc gtaagactcc ggggtctcgt cgtcgtcgaa ccccaccacg ccgccgacga
     2521 tgacttcctc ttcgggaagg gccgacgctg gttcgtccgc ggtgggggcg aagtggtcgg
     2581 cggcagcttc gagttcctcg tccgacggcg cggcggtcac gacgacctcc tcctcgatcg
     2641 tggacatggg ctgggtgtcg gtgccgatga tgccggagac gtccggttgt tcgtcgtcct
     2701 cgacgagcga ccatccgtcg cccggctccg cgtccgcggg gtcgtcggag gcgaacagcc
     2761 ccgcctggtc cacggcggtc tcgccgatgg gcgcgtcgct gacctggagg tcatcgaggc
     2821 tcaccacctc ggtcgtggtc tcgagctggg gcgcctcgtc gggctcgtcg gtgccgatgc
     2881 cttcgatcgt gtcgaggacg gtggtgtcgt accactcctc atcgtcgtcg tcctcggccg
     2941 aggccgagac gggcgcgggt tcgtcgagca cggtgggttc gtcctcggag ggctccgggg
     3001 ttcgcgaccc ggtcgcgagc ggagcggggt ccggcgcccg gaccgccacg tgctcctcgc
     3061 tgggcgtttc gcgggcaccg gccgaagccg gctcgtccca atcgacgtcc ttgatgggcg
     3121 cgaagaactt gtccaggtct tcgaacagat cgtcgccgtc gcgcttgtcg tccgccacta
     3181 gacctccatg agctcctgtt ccttgtggcc ttggatctca tcgatctcgg ccacgaagcg
     3241 gtcggtcact ttctggagct ccttctcccc tctcgcaagg tcatcttggg agataccgcc
     3301 ctctcgctca agcttctcca tctcttcctt ggaatgtcgc cggacgttgc ggaccgccac
     3361 ccggccctct tctgaccggt gatggagctg tttcaccagg tccttgcgcc gttcctcggt
     3421 gagcggcggg aaggccagac ggatcacctg gccgtcgttg gacggggtga tccccaggtc
     3481 ggaggactgg atcgccttct cgatcgcccc gatggcgccc ttgtcgaagg gggtgaccgc
     3541 gagcagccgt ggctctggga ccgtgaccga ggcgagctgg ttcagcggga ccggggtgcc
     3601 gtagtactcc acggtcacgc ggttcaacac cgaggcggat gcgcgaccgg tccggatgcc
     3661 ggcgaggtcc tccttcaggt gcgagaccgc ctgctccatc ttgtggtcgg cgtccttgag
     3721 ggcggaatcg atcgtcatcg ggctccttgt cagtggacga gcgtgccgat cggctcgccg
     3781 ccgaccacgc gtcggatgtt cccttcttcg cccaggtcga acacgatgat gggcagcttg
     3841 ttctccatgc acagcgagat ggcggtggcg tccatcacct ggtagccctc cttgaggacc
     3901 tccacgtact cgatggcgtc gaaccgggtg gcgctggcat ccaggtgcgg gtcggcggtg
     3961 tagaccccgt cgaccttggt gcccttcagc agggcctcgg ccccgatctc gagggcacgc
     4021 tgggccgcgc acgtgtcggt ggagaagaac ggcaccccca gcccggcggc gaagataacg
     4081 acgcgcccct tctccaggtg ccggcgagcg cgcagtggcg tgaacggttc cgccagctgg
     4141 gacatggaga tcgccgtctg cacccgcgtg gtgacgtgga tcttctccag ggcatcctgc
     4201 agcgccaacg cgttcatgac cgtggcgagc atgcccatgt agtcggcgcg gttgcgctcg
     4261 atgccgggtg cctgccggcc gcgccagatg ttgccgccgc ccaccacgac ggcggtctgg
     4321 accccgaggt cgacgacctc agccagttgc cgcgccacga gggcggtggc gtcggtgttg
     4381 atgccgcttt cggcgtcggc gggagcgaag gcctctccgg agagcttcaa caggacccgg
     4441 ttgtactggg gtcgggtccc gtgctgttgg tcggccatag gctcctcggt cccccctcgg
     4501 acctcgctct tcgtgcctac tcggtgtctt cgccgagctt gtaccggacg aaccgcttca
     4561 ccgcaacctt ctcacctacg cgcgcggagg tctcgtcgag cagctgctgc acggtcttgc
     4621 tgtcgtcctt cacgaacggc tggtcgagca gcacgatctc cttgaagtag ctgttgatct
     4681 tgccttcgac gatcttgtcg accacgttct cgggcttgcc cgattcctgc gcctgcttgg
     4741 cgaagatctg gctctcgtgg tcgatctcgt cctgcgagac ctcctcgcgg ctgacgaagc
     4801 gcggcgcggc ggggctggcg atgtgcagcg cgatgtcctt cgcgagtgcc ttgaactcgt
     4861 ccgtgttggc gacgaagtcg gtctcgcagt tgacctcgac gagcaccccc accgtgttgt
     4921 tgaagtggat gtaggcctcg atcaggccct ggttcgccga gcggccggcg cgcttggccg
     4981 ccgaggcgag gcccttctgg cgcaggagct cgacggcttt ctccatatcg ccgccggtct
     5041 cgaccagcgc cttcttgcag tccatcatgc ccgcgcccgt tcggtcgcgg agctccttga
     5101 cctgtgcagc tgagatgtcg ctcatggggg tgtcttactc ctgggtggta tcggcggcga
     5161 cgggctcggg cgtgggggcc acctcggcct cgggagcctc cgtcgtggcg gctggtgccc
     5221 ccgcttcggc ctcgaccgga gcgccgggct cctcagcggc ctcagccgct gccgcggccg
     5281 tttcggcatc cgaaggcgcg tccggctcga agaccgtgga tgcggcgatg gccgcggcgt
     5341 cctcggcgct gggcgtctcg tgctcttcca cacgcgctgg cgcggcggtg gccttggggc
     5401 cggtggcctt gggcgcagcg ccggtaaccc gctcgacggc ctcgtccttc gccatgccgt
     5461 agccggcggc cagcgcgtcg gcgacgacac gggtgaccag gttcacggcg cggatggcgt
     5521 cgtcgttgcc cgggatcacg aagtcgacct catcgggatc gcagttggtg tcgacgatgg
     5581 cgatcacggg gatgttcagc ttgcgggcct cgttgacggc gatgtgctcc ttcttcgtgt
     5641 cgatcacgaa gatcgcgtcg ggaagccgct cgaggtcctg gatgccgccg aggttgcgct
     5701 ggagcttgtc gcgctcgtgg cgcaggcgga tcgcttcctt cttcggcagg tagtcgagcg
     5761 caccggtgcg atccatctcg cgcagttcgc gaaggcgcag caggcgcttg gacaccgtgg
     5821 tgaagttcgt gagcatgccg ccgagccagc ggtggttcac gtagggcatg cccacgcggg
     5881 cggactgctc ctgcacgacc tcctgggcct gcttcttcgt cccgacgaac aggacgatgc
     5941 cgcgacgacg gcccaggttc cgcacgaact cgtaggtctc ctcgaggccg gacagggact
     6001 tctcgaggtc gatgatgtag atcccggaac gctccccgta gaggtagcga tgcatcttcg
     6061 ggttccaccg gcgtgtctgg tggccgaaat gcacgcccgc ctcgaggagc tcccgcctgg
     6121 tgacgacagg catggtgcct ccttcgttcg gttgtcatcc tccgctcgga tcaacccgtc
     6181 accgacccgc ccgtgagggc gcgggacctc ggcgatgcgg tcctcgagcg tgtgtgctcg
     6241 gcgcgaacgc gccggcgcgg ggagtttagc agcggggcag atccggcgac ccgagggtca
     6301 tcgacggcgg ccgcgagccg ccggctggcg ccggggcgcc gcttcgggca ccacgtcggc
     6361 gatgctcagg taccggaaca ccagggccgc gaggaccgcc acatgggcca agcccaccac
     6421 gatgcccacc acccaggccg cgatcgacgg gttcacgccc accatggcgc cgggcttgcc
     6481 ggggatggcc aggaccgcca cgaacgtgac cacgtagatg gccgcgaaca tcaggttgcc
     6541 ggcccccggc aggcgtcccg cccggaccga tcggcccagg gtgtcggcga ggccccggcg
     6601 ctcggtggcg gcgatcgccg gcgcgaaccc gagcaggtac acgccggcca ccagcgacgc
     6661 gagcaggatc agcagcccga acccctgacc cagcagcggc ccgacgaagc tcgaagccat
     6721 gagcaggccg aggcaggcca cgttcacggc gagcgtcacg ggcaggatcc gcagcgagcg
     6781 aaccacggac cagcgggtgg ccgatccggt ctgcaggacg tcgaccacca tgaccgtgag
     6841 caccgcttgg atgatggcgc ggagcccgat gaacacgagc agtgccagga agccggtggt
     6901 gaccccgaag acgctgatcg acaggttgag gtcgttgaac gtgcccaccg ggggcacccc
     6961 gagcacgttc acgagcagcg cgaacggccc ctcatagccg aggccgacga gcacgagcca
     7021 catcgccagg agcagcagga tggaggtgag gaccagcggg atcgacgatg ccacggtcac
     7081 gcacccgcgg ccgaggctcg tgaggatcga cggcatcggc gtcgagcccg ggacgcgggg
     7141 cgccaggatc gagtcgaaca gccctcgtcg gcgcggcggt tgggccacgt cggtcgatgc
     7201 cgaaggggac tctcgatagc cgctggggcg tttcttgttc ttgttcgtca ccggtcgtcc
     7261 tcgtcatcat catcctgcgc gggatccccg tcgtcgtcct cgtcttcttc gtcgtcttct
     7321 tcgtcgtcgt cgtcgaggtc gccttcgagc ggatcttcat cattcgggaa cgccgggccg
     7381 atgcggccga cgaggacccg gcccgcgagc aggacgatgc ccgcgaccac cagcgcggcg
     7441 acggcgaagg cgccgaggag caggccaagg ttcaacgtcg tgggcgacac ctcgcggacg
     7501 agcgccacgc cgtcgtcatg gacgaacgcg accaggcccg cgccggcgcc gccgcgaacc
     7561 gcgatcacgc gatcaccggc gacggcgagg gcccggagcg gggagccgtc gccgtcgtcg
     7621 cgccacacca ggtcgccgcc cgaggcctcg agggcgacca gcgagccgtc gatcgtgccg
     7681 acgagcacgt ggtcgccgac ggcgatcggc gcggtgcgca ggacgccttc gttcagcgcg
     7741 aactcccacg cccgggtccc ggtggcggga tcgagccgat aggtctcgcc ctgggcgtcg
     7801 gtgacgtaga ccgcgtcgtc ggtcaccacc ggcggcgtgg tcgcggtgag gtcgaagaac
     7861 cgtgggaagc gtgtgcgcca ccgtcgaccg ccggtgtcga ggtcgaacgc gtcgatgctc
     7921 gactcctgac caccggtgaa ggcgacgtac accgtcccgc cggcgatcga cgccgtggac
     7981 acgatggcgg cctggctctc gtcgtcggcg cgccaacgct cttcgccgga ggacgcatcc
     8041 agggccacga ccgtggggtg gggctcctgc cggctcgcct ggaggccgac caccaccgtc
     8101 ccggccgtga aagcgggctg ggtggcgacg ggccgtccca gctcagcggt ccacgccacc
     8161 ttccccttcg cgaggtcgac ggcgtagatc gtgccgccgt tcactccgag gtaggccatg
     8221 ccgtcgccca cggtgacccc ggtccggctc acggcgctga gcgccaccgg gtcccacagc
     8281 ggctcctggg tatccagatc gaacgcggcc aggtgcgaat cgaaccgttc ttcctgggtg
     8341 gcaccgtcac tggatgacgg gctcgcggaa ccggaggccg aggcggaagg agacgcagac
     8401 ccagcggcat cagggggacc gtcgccgaat ccttccgtgt agaccacc
//
LOCUS       DATDBZ010000171         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_33624,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000171 DATDBZ010000000
VERSION     DATDBZ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33624"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2339)
                     /gene="fdhF"
                     /locus_tag="VIX62_04215"
     CDS             complement(<1..>2339)
                     /gene="fdhF"
                     /locus_tag="VIX62_04215"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY5859467.1"
                     /translation="KCIVCSRCVRACDEVQGTFALTIAGRGFDSRVSAGQDEPFLESE
                     CVSCGACVQACPTDALQEKTLIQLGPPNRSVLTTCAYCGVGCSFKAEMQGDTVVNMVP
                     YKNGGANEGHSCVKGRFAWGYANHVDRVLHPMVRDTIDEPWREVSWDEAIAYTASRFK
                     AITAQYGATAIGGITSSRCTNEEVYLVQKMVRAAFGTNNVDTCARVCHSPTGYGLKTT
                     FGTSAGTQDFASVEHADVIMVIGANPTDGHPVFASRMKQRLRQGAKLIVIDPRRIDLV
                     RSPHIEASHHLQLLPGTNVAVINALAHVVVTEGLMDQAFVDERCEDVGEWAEFIARPE
                     NSPEAMEPITGVPAAELRAAARLYATAPNAAIYYGLGVTEHSQGSTMVMGMANLAMAT
                     GNIGREGVGVSPLRGQNNVQGSCDMGSFPHELSGYRHVSDPTVRELFADLWGTPVHPE
                     PGLRIPNMLSAAIDGTFRGIYIQGEDLAQSDPNTQHVTAALMAMDLVVVQDLFLNETA
                     KYAHVFLPGTSFLEKNGTFTNAERRINRVRPVMASATGKQEWEVVVELSHAMGYDMAY
                     ADESEIMDEIAALTPTFAGVSFKKLDELGSIQWPCNDAAPDGTRTMHVDRFVRGKGHF
                     VPTAYVATDERANRKFPLLLTTGRILSQYNVGAQTRRTANNTWHPEDVLDIHPSDAEL
                     RGIHDGDWVSLASRVGETTLKANLSERMPPGVVYTTFHHPVTGANVVTTEFSDWATNC
                     PEYKVTAVQVTPAPTTKPLIMHSVPLESTLVEAGRSEGV"
BASE COUNT          348 a          783 c          804 g          404 t
ORIGIN      
        1 gcacgccttc ggaccgccca gcctcgacga gcgtggactc gagcggcacc gaatgcatga
       61 tcaacggctt ggtcgtaggc gccggcgtga cctgcacggc cgtgaccttg tactcggggc
      121 agttcgtcgc ccagtccgag aactcggtcg tgacgacgtt ggcgcccgtg accgggtggt
      181 ggaacgtcgt gtagacgacg cccggcggca tccgctcgga caggttcgcc ttgagcgtgg
      241 tctcgccgac ccggctcgcg agcgacaccc agtcgccgtc gtggatcccc cgcaactcgg
      301 catcgctcgg gtggatgtcc aggacatcct ccggatgcca ggtgttgttc gcggtgcgac
      361 gggtctgcgc accgacgttg tactgcgaca ggatccgccc ggtcgtgagc agcagcggga
      421 acttgcggtt ggcccgctcg tccgtggcca cgtaggcggt cgggacgaaa tggcccttgc
      481 cgcggacgaa ccgatccacg tgcatcgtcc gcgtcccgtc gggcgcggcg tcgttgcacg
      541 gccactggat gctgcccagt tcgtcgagct ttttgaagga cacgccggca aaggtcgggg
      601 tcagggccgc gatctcgtcc atgatctcgg actcgtcggc ataggccatg tcgtagccca
      661 tcgcatgcga gagctcgacg acgacctccc actcctgctt gcccgtggcg gacgccatga
      721 ccggccggac gcggttgatc cggcgctcgg cattcgtgaa cgtcccgttc ttctcgagga
      781 acgacgtgcc gggcaggaag acgtgggcgt acttggccgt ctcgttgagg aacaggtcct
      841 gcaccacgac gaggtccatc gccatgagcg cggccgtgac gtgctgggtg ttcgggtccg
      901 actgggcgag gtcctcgccc tggatgtaga tcccgcggaa cgtgccgtcg atcgcggcgc
      961 tcagcatgtt cgggatgcgc aggcccggtt ccggatggac cggggtcccc cacaggtcgg
     1021 caaacagctc gcggacggtc ggatccgaca cgtgccggta gccggacagc tcgtgcggga
     1081 acgagcccat gtcgcacgag ccctgaacgt tgttctgacc gcgcagcggg ctgacgccga
     1141 ccccttcgcg accgatgttg ccggtcgcca tcgcgaggtt ggccatcccc atgaccatgg
     1201 tcgagccctg gctgtgctcg gtgacaccca ggccgtagta gatcgccgcg ttcggggccg
     1261 tcgcgtagag tcgcgcggcc gctcgcagct cggcagccgg taccccggtg atcggctcca
     1321 tcgcctcggg actgttctcg gggcgggcga tgaattcggc ccactccccg acgtcctcgc
     1381 agcgctcgtc aacgaaggcc tggtccatca gcccctcggt gaccacgaca tgggccagcg
     1441 cgttgatgac cgccacgttc gtaccgggca ggagctggag gtggtggctc gcctcgatgt
     1501 gcgggctgcg cacgaggtcg atccgccgcg ggtcgatgac gatgagcttc gcgccctggc
     1561 gcaggcgttg cttcatccgc gacgcgaaga ccgggtggcc gtcggtcgga ttggcaccga
     1621 tgaccatgat gacgtcggcg tgttcgaccg acgcgaagtc ctgcgtcccg gcggacgtcc
     1681 cgaacgtcgt cttgaggccg tagccggtcg gcgagtgaca gacccgggcg caggtgtcga
     1741 cgttgttcgt gccgaaagcc gctcgcacca tcttctggac gaggtagacc tcctcgttcg
     1801 tgcagcgcga cgacgtgatc ccgccgatgg cggtcgcgcc gtactgcgcg gtgatcgcct
     1861 tgaagcggct cgccgtgtag gcgatggcct cgtcccagct gacctcacgc cacggctcgt
     1921 cgatcgtgtc gcgaaccatg gggtgcagga cgcgatccac gtggttggcg tagccccacg
     1981 cgaagcggcc cttgacgcac gagtggcctt cgttggcccc gccattcttg taagggacca
     2041 tgttcacgac cgtatcgccc tgcatctcgg ccttgaacga gcacccgaca ccgcagtacg
     2101 cgcaggtcgt caggacgctc cgattcggtg ggccgagctg gatgagggtc ttctcctgga
     2161 gcgcgtcggt cgggcaggcc tgcacgcacg ctccgcagct cacgcactcg gactcgagga
     2221 acggctcatc ctggccggcc gagacgcgcg agtcgaaccc gcggccggcg atcgtcaggg
     2281 cgaacgtgcc ctggacctcg tcgcacgccc ggacgcagcg cgagcacacg atgcacttg
//
LOCUS       DATDBZ010000172         5063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1491025, whole genome shotgun sequence.
ACCESSION   DATDBZ010000172 DATDBZ010000000
VERSION     DATDBZ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491025"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VIX62_04220"
     CDS             complement(<1..319)
                     /locus_tag="VIX62_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY5859468.1"
                     /translation="MNERLDLQRITSAILRRWMVVLAVTVGIAALGNLASKATAATYE
                     ATTSVLVGQEPPGVIFENSDRATKEQLALTFAELAVRAPVLEPAASALGMESWRPLRS
                     RVTI"
     gene            complement(316..1623)
                     /locus_tag="VIX62_04225"
     CDS             complement(316..1623)
                     /locus_tag="VIX62_04225"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY5859469.1"
                     /translation="MSAVLPARAAAPTRLQRVRESTGLDTIELMFLLSALLAPADIEV
                     FGSLTISDVLVTGVAYLLVADGRRIAALPPLFIPAILILLGAAAAGGFRASHPYETLT
                     QLFQLGFILLIQLSVILTVVTTRRLLHLSLLMVAIGSLAGNVASFVMQQAQGADRILA
                     FYSDNPNRLSYPTAYLLPFVLHYITVLWERGLRWRAFWVGGSLLYLMIWALAASASRG
                     GALATVVGLVAYVVFADGLRGSLRRAMLTALGLGAVVLVLFNTTLFPVTLQDRVDRSL
                     TTDAAEQTTLLADRERLADAAVAGIIDSPIIGTGLDNFRHVAQKYHPQATPQQPHNMW
                     LALMAETGVVGALAAAFLFTTWFELLIRARRYTRDRELQRLAWASVSSMMAVMAIFMT
                     IPIMLHRHYWLLYGLGLAIVELIRRESETAFAATGFTARLDAR"
     gene            complement(1620..3212)
                     /locus_tag="VIX62_04230"
     CDS             complement(1620..3212)
                     /locus_tag="VIX62_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859470.1"
                     /translation="MEIVGAEPEESSRSPSSNDPDAARSGNLSRKASLNAAAAGLDFA
                     ARTVVELILNPLMLRGLGDYLFGAWRILWRLTGYLWATSGRAAQALQSAIANRLHSDD
                     TDEKRRLVGASIVVWALYLPLLTVVTALAMWTLPDLLDAPAQYVAPLRWTILLLGVDS
                     VMVSLLTIPRATLQGENLGYRRMGLSTALVLISGVLTAVALWLDLSIVGVAAVNLVAT
                     IYTGIVFWRITRAHVPWFRADWPLRREVRWFFGLSSWFMVWKLVNQLMMSGDVLVLGV
                     VGSVELVSIYALTKFIPEAMLPLLSMLVMGGIPGLGGIIGAGDHPRARRVRGEVLALT
                     WLLALASGVAILMWNESFVALWVGEQYFAGGTELLLLMISLLQLALIRSDAFIIDLTL
                     KVRAKTLTGLASSVIALVVAGLLIRNHDMGITGLCIGLITGRSVLSVIYPALIGRNIG
                     HPLRDQLRSSIRPGLVTIALFGAAAVGRQEISTRSWPVLIVGSAVTGLIALALAFMVG
                     LDPTTRHRVGKRVRVLVGRAGR"
     gene            3345..4880
                     /locus_tag="VIX62_04235"
     CDS             3345..4880
                     /locus_tag="VIX62_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859471.1"
                     /translation="MSRGRRPDAGRWAVRGGRGRMGIIVLLAGLVLSSASCSSDRRDE
                     DGFAPTTKPEDGAAQPAGDVLIGAGEIGGCTPETALTASLLESYPQATVVTFGGHTSE
                     PATADTYASCYGTTWGGALDRTRPAVGKTDLAGDGGAAYYDYFGAAAGAAGSGWYSYD
                     VGSWHLVVLNSDCDQVGCKDRSDQVEWLRADLASSRAACIGAYWEKPRFSSGSHGNTD
                     SVQAFWDLLHEHGADFVLNGYDAGYERFGPLDAVGVHDPANGVRQFVVGTGGAPRAGF
                     ESIQPNSEVRDATSWGVIKMTLHEGAYSWEFLAAEGSDLADFGTARCSGAPPEAGGAK
                     TAAVMAMAGSEDDAEERRSGRVSVDSTDLEMILDLDEEQTVGLRFPSVEIPEGKKLVE
                     AYVQFVAGAPSVGEANFRIAADVSSGAPFSDLRGDLSRRTKSESFVTWLPANWNVGGE
                     LGPAQRTPDLRRVIAEVTDRPLWRSGDSVVLLITGSGLRIAQSVDRALNRAPVLHLVF
                     EER"
BASE COUNT          898 a         1652 c         1744 g          769 t
ORIGIN      
        1 ggatcgtcac ccgcgagcgc agcgggcgcc agctctccat gccgagtgcg gaagctgccg
       61 gttcgagtac cggcgcgcgg accgcgagct cggcgaaggt caaggccagc tgctccttcg
      121 tggcacggtc gctgttctcg aagatcacac ctggcggttc ctggccgacg aggacgctgg
      181 tcgtcgcttc ataggtggca gccgtggcct tgctggccag gttcccgagc gcggcgatgc
      241 cgacggtcac ggcgagcacg accatccacc ggcggaggat cgcggacgtg atccgttgga
      301 gatccaagcg ttcgttcatc gtgcatccag tcttgccgtg aatccggtcg cggcgaacgc
      361 agtctcggat tccctgcgga tcagctccac aatggccagg ccgagcccgt acaacaacca
      421 gtagtggcga tggagcatga tcgggatcgt catgaagatg gccatcacgg ccatcatcga
      481 gctcacgctg gcccatgcca gacgctgcag ctcccggtcg cgggtgtacc gacgggctcg
      541 aatcagcagc tcgaaccatg tggtgaacag gaaggctgcc gccagtgcgc cgacgacgcc
      601 ggtttccgcc atcagcgcca gccacatgtt gtgcggttgt tgtggtgtcg cctgtgggtg
      661 gtacttctgg gcgacgtggc ggaagttgtc cagtcccgtg ccgattatcg gcgagtcgat
      721 gatgcccgcc acggccgcgt cggccaggcg ctcccggtcg gcgagcaacg tcgtctgttc
      781 ggcggcgtcc gtggtcaacg agcgatccac tcgatcctgc agcgtcaccg ggaacagcgt
      841 cgtgttgaac agcaccagca cgactgcgcc gaggccgagg gcggtgagca tcgctcgccg
      901 caggctgccg cggaggccgt cggcgaagac gacgtaggcc accaacccaa ccaccgtcgc
      961 gagtgcgccg ccgcgggagg ccgacgccgc gagcgcccag atcatcagat atagaagact
     1021 cccgccgacc cagaacgccc gccagcgcaa gccgcgctcc cacagcacgg tgatgtagtg
     1081 cagcacgaac ggaaggaggt acgccgtcgg gtaagaaagt cggttcgggt tgtcgctgta
     1141 gaaggcgagg atccggtcag cgccttgtgc ctgctgcatc acgaaggagg cgacattgcc
     1201 ggccagcgag ccgatcgcga ccatcaacag gctcaagtgg aggaggcggc gcgtcgtgac
     1261 gacggtgagg atcaccgaca gctggatcag caagatgaaa ccgagctgga agagctgggt
     1321 caaggtctcg tacggatggc tggccctgaa cccgccggcc gccgccgcgc cgagcaggat
     1381 caggatggcg ggtatgaaca gcggcggtag tgcggcgatc cgcctgccgt cggcgaccaa
     1441 caggtacgcc acgccggtca cgagcacgtc tgagatggtc aggctgccga acacctcgat
     1501 gtcagccggt gcgaggagcg cggagagcag gaacatcagc tcgatcgtgt ccagcccggt
     1561 gctctcccgg acccgttgca ggcgtgtcgg cgccgccgcc cgcgccggga gcacggcgct
     1621 catcgacctg cccgcccgac gagcacgcgc acccgcttac cgacccgatg gcgggtggtc
     1681 gggtcgagac cgaccatgaa ggcgagtgcc agcgcgatca gccccgtgac ggcggagccg
     1741 acgatcaaca cgggccacga ccgggtcgag atctcctgac ggccgactgc ggccgctccg
     1801 aagagggcga tcgtgacgag gcccggtcgg atcgatgagc gcagctggtc gcgtaacggg
     1861 tgcccgatgt tcctgccgat caacgcaggg tagatcacgc tcaggacgga tcgtccagtg
     1921 atcaggccga tgcacaagcc cgtgatgccc atgtcgtggt tccggatcag caagccggcg
     1981 accaccagcg cgatgaccga ggaggcgagg ccggtgagcg tcttcgctct caccttcaac
     2041 gtgaggtcga tgatgaacgc gtcgctgcgg atcagggcga gctgcagcag cgagatcatg
     2101 agcagcagca gctccgtgcc accggcgaag tactgttcgc cgacccagag cgccacgaac
     2161 gactcgttcc acatgaggat cgcgacccca ctggccagcg cgaggagcca cgtcagggcg
     2221 aggacctcgc cccgtacgcg ccgcgctcgt gggtggtcgc cggcgccgat gatcccgccg
     2281 agtccgggaa taccgcccat cacgagcatc gacaggagtg gcagcatcgc ctcggggatg
     2341 aacttcgtca gcgcgtagat gctgaccaac tcgaccgagc cgacgacgcc gaggaccagc
     2401 acgtctcccg acatcatcaa ctggttgacg agcttccaca ccatgaacca ggagctgagg
     2461 ccgaagaacc acctgacctc ccgccggagc ggccaatcgg cgcggaacca tgggacgtgg
     2521 gcgcgagtga tcctccagaa cacgatgccc gtgtagatcg tcgccacgag gttgacggcg
     2581 gcgacgccga cgatgctcag atcgagccac agcgcgacgg cggtcagcac accggagatc
     2641 agcacgagcg ccgtggagag acccattcgt cggtagccga ggttctcgcc ctgcaacgtc
     2701 gctcgtggga tggtcaggag gctgaccatg accgaatcga cgccgagcag caggatcgtc
     2761 caccgcagcg gcgcaacgta ttgggcggga gcatcgagga ggtcgggcag cgtccacatc
     2821 gccaaagcgg tcaccaccgt gagcaacggt aagtacaacg cccacacgac gatcgaagca
     2881 ccgaccagcc gtcgcttctc gtcggtgtcg tcgctgtgca gccgattcgc gatcgccgac
     2941 tgcagcgcct gggcggcgcg gccgctggtc gcccagagat accccgtcaa ccgccagagg
     3001 atccgccagg cgccgaagag gtagtccccg agtccccgca gcatgagggg gttcaggatc
     3061 agttcgacga cggtacgggc ggcgaagtcg aggcccgccg cggccgcgtt cagcgatgcc
     3121 ttccgggaca ggttccctga gcgcgctgca tcgggatcgt tgctgctcgg ggatcgggag
     3181 gactcctccg gctccgcacc aacgatctcc atccgcgcgt cctttactcg ccggctgccc
     3241 acactgccca ctgcagagta cccctcgtgg tcggcagccg cccgccaggc tggtcatgca
     3301 cgccgttaga gatggacgga gacgatgtct cttacactca cggcgtgtct cgtggacggc
     3361 ggcccgacgc gggtcgatgg gcggtgcgtg gcggacgtgg acgtatgggc atcatcgtgc
     3421 ttctcgccgg tctcgttctg tcctccgcgt cgtgctcctc cgaccgccgt gatgaggacg
     3481 ggttcgcccc gaccaccaaa ccggaggacg gggccgccca accggccggc gacgtgctga
     3541 tcggagcggg tgagatcggt ggatgcacgc cagagaccgc gctgaccgcc tcgctacttg
     3601 agtcgtaccc gcaggccacc gtcgtcacgt tcggtggaca cacctcggag ccggccaccg
     3661 cggacaccta cgcctcgtgc tacgggacaa cctggggagg cgcgcttgat cgcacgcgtc
     3721 ccgccgtcgg caagacagac ctggcgggcg acggcggcgc cgcgtactac gactacttcg
     3781 gcgccgccgc cggtgctgcc ggatcgggtt ggtacagcta cgacgtcggc tcgtggcacc
     3841 tggtcgtgct caacagcgac tgcgatcagg tgggctgcaa ggatcgctcc gaccaggtcg
     3901 agtggctccg agccgatctc gcgtcgagtc gcgccgcctg catcggggcg tactgggaga
     3961 aaccgaggtt cagctcggga tcccacggca ataccgactc cgtccaagcg ttctgggacc
     4021 tgctgcacga gcacggcgcg gacttcgtcc tcaatggcta cgacgccggc tacgagcgtt
     4081 tcggaccgct ggacgcggtc ggagtccacg atccggcgaa cggcgtccgc cagttcgtcg
     4141 tcggtacggg cggggcgccc cgggctggtt tcgagtcgat ccagccaaac agcgaggtcc
     4201 gtgacgccac gtcatgggga gtcatcaaga tgacgctcca cgagggagcg tactcgtggg
     4261 agttccttgc cgccgaggga agcgacctgg cggacttcgg tacggcacgg tgcagtggtg
     4321 cgccgccgga ggcgggtggg gcgaagaccg ctgctgtgat ggcgatggcg ggcagcgagg
     4381 acgatgccga ggagcgccgg tccggcaggg tgtccgtgga cagcaccgac ctcgagatga
     4441 tcctcgacct cgacgaggaa cagaccgttg gtctccgatt cccgtcggtc gagattccgg
     4501 aagggaagaa actcgtggaa gcatacgtac agttcgtcgc cggagcgccg agtgtcgggg
     4561 aggcgaactt ccggatcgcc gccgatgtct cctccggcgc gccgttcagc gacttacgcg
     4621 gtgacctctc ccgccggacc aagtccgaat cgttcgtcac ctggctgccg gcgaattgga
     4681 acgtcggcgg cgagctcggt cctgcgcaac ggacccccga cctccggcgc gtgatcgcgg
     4741 aggtcaccga cagacccctc tggcgcagcg gtgattcggt cgtgttgctc atcaccggat
     4801 cggggcttcg cattgcgcag tcggtcgatc gggcgctgaa ccgggcgccc gtgctgcacc
     4861 tcgtcttcga agagcggtag tcagagaggc ataaggaccg accggcgtcc ggcgtccggc
     4921 gtccggcgtc cggcgtcgtt tcgtggttga gctggcgcgc tctgccgtgg cggtggctgt
     4981 cgagctaccg caggaggcaa cggacagcgg gcgaaggcac gacaccgcag gtcagttgcc
     5041 gatactgcag gttcgaacgg acg
//
LOCUS       DATDBZ010000173         5713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2515007, whole genome shotgun sequence.
ACCESSION   DATDBZ010000173 DATDBZ010000000
VERSION     DATDBZ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2515007"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..967)
                     /locus_tag="VIX62_04240"
     CDS             complement(44..967)
                     /locus_tag="VIX62_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY5859472.1"
                     /translation="MDVNIALIGAIVILLLVIVFLAKAIKVVPEFQRLVVFRLGRLLG
                     QKGPGLILLIPFVDKATVVDLREFYLEIPRQDSITKDNAPIAVDFVMFYKVMDAQTSV
                     VQVGNFAGAALNIASTTLRAVIGDIPLDDMLAKRDEINTLLRLKLDEVTERWGVKVTN
                     VEIREIIPPAAVQEAMTRQMSAERTRRAVVTEADGAKAAAITVAEGNKEAAILNAEGS
                     KQSNILTAEGERQAAILRAEGYALAIRSINESASVADLKTMQLQYLEALKAIGSSSSS
                     KVVVPMELSGLAAGIAAIAEAAAPTGGGSSR"
     gene            complement(967..2307)
                     /locus_tag="VIX62_04245"
     CDS             complement(967..2307)
                     /locus_tag="VIX62_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEY5859473.1"
                     /translation="MVTAILIAVTGAFVLAGTADAQDGSAEPVPLLDLEGVVDPFLAD
                     YIEHGIEVANGDDPAPAVMIEIDTPGGLDSSMRQITQAILNSKVPVICYVSPQGARAA
                     SAGAFVLLSCPVAAMAPGTNVGAATPVGLSGAIGSDKAVNDAAAYIRSLAETYGRNAD
                     MAESFVRSAESITAETALSADVIDAIAPTAEALLVDIDGRQVRLGDGTTTTLDTQGWV
                     LEDQPMGGFIGFLHSLLDPNLAFIFFWVGLALIVLELLVPGHVFSGTIGTILLILSVV
                     SFGLLPVQLVGIALLIGAVVLMIVELHAPGFGIWGFAGLACLVLGGWFLYDRSQGVSV
                     SPAVLAIVAIFIGAFFFLVLTKVLKMRHMAPAQGPEVVVGKEGVVIGRGLDPKGIVRV
                     ASEEWKASTSDGSSLPAGARVVVTRLDGLALTVDAANDEGATAGTAPAEGGRNT"
     gene            2560..3081
                     /locus_tag="VIX62_04250"
     CDS             2560..3081
                     /locus_tag="VIX62_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5859474.1"
                     /translation="MEGLLAGNDDAIRTLYSRFGRPVYSLGLRLLGTRESAEELTQDV
                     FLTAWRKAARFDPARGRLSTWLMTIAHNLAVDRLRRETGVSRPTLVLVDEVPDAPGLD
                     EEAVLIERDSALRALESLSPAERLLVGRAYFRGQTAREIAEADGIPLGTVKTRLRSAM
                     IKVRKANEGKDLL"
     gene            3078..3875
                     /locus_tag="VIX62_04255"
     CDS             3078..3875
                     /locus_tag="VIX62_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY5859475.1"
                     /translation="MNCVTVRDGLTERALGTLPARERARIDRHLVWCAACRKEAAEME
                     SAAAVFAFGVDQTEPDPALEASVIASINHASGRRAPHTAPRRGRLAVVAVLAAMLAVS
                     GLGWGAVMAGRAARSDEAALVTTLRQQSAAEKFSRILSSAEFADPEGEVFLGTLGPTN
                     EGGGGGSALTLVSPTITDMAVVLVNGVPPAQRKGVPFTVWLRGPDGASLAAGQIDALD
                     AAGSGIMWEDIDRDLGVFDRVVVRDTGGHAVMRGIIATRAPLSSPSP"
     gene            3922..4932
                     /locus_tag="VIX62_04260"
     CDS             3922..4932
                     /locus_tag="VIX62_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5859476.1"
                     /translation="MRFGLSLPHYGFSLPGSAPITFGAMAAWAHRAEELGFDSVSISD
                     HFFYSFGRYGADPQPIASLEPMTALAGLAAVTDRVRLGALVLCAPFRHPSNLAKMAAT
                     IDQLSGGRLDLGIGAGWLEQEFSAFGYPFGTVGERFEALEETLQVLEALFSGAPATFD
                     GPTVTLRDARLLPAPAQEPRIPVWVGGKGGPRLLRLAARHADGWNAVWRWAPQAYADR
                     VAAARQICEQEGRDPATFRLSVGLSCLLGEDEAAARAAFERGRASMPGDAMAAETDAS
                     WRADTLSGTPEQALERLHAFGSLGVEEVVIAPWVLPFAVPEPEQVELFAERVMGPFRA
                     GA"
     gene            4929..5144
                     /locus_tag="VIX62_04265"
     CDS             4929..5144
                     /locus_tag="VIX62_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859477.1"
                     /translation="MRMEPAAAILAVLEAADGPLHWTVIQDRALRAGYLDPFETPDVR
                     GVVGRTLRELASAGTIVRVEQGTYRSV"
     gene            complement(5151..5390)
                     /locus_tag="VIX62_04270"
     CDS             complement(5151..5390)
                     /locus_tag="VIX62_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859478.1"
                     /translation="MAEKTILVCDTCGRPAAETITIKVARGNYVKDLCSTHVNEAIAG
                     ARKPRPGRRKAVVGSAKPKVSAAPKKRGRPRKKPA"
     gene            complement(5459..>5713)
                     /locus_tag="VIX62_04275"
     CDS             complement(5459..>5713)
                     /locus_tag="VIX62_04275"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEY5859479.1"
                     /translation="QQLVDDGYDVDAVLSTDDLVSGDDVFFAATGITEGNLLRGVRYW
                     PDGATTHSMVMRSRSGTVRWVEAEHRFEKLERFSPIAYRR"
BASE COUNT          858 a         1948 c         1985 g          922 t
ORIGIN      
        1 ccttccggat gggtcagcga gatcgtgatg aggtcggcgg cgcctagcgg ctcgaccccc
       61 cgccggtggg tgcggcggcc tcggcgatcg cggcgatccc cgccgccagg cccgacagct
      121 ccatcgggac cacgaccttc gacgaggacg aggaaccgat cgccttcagc gcctccaggt
      181 actggagctg catcgtcttg aggtcggcga cgctggccga ttcgttgatc gatcggatcg
      241 cgagcgcata gccctcggcc cgcaggatcg cggcctgccg ttccccttcg gccgtgagga
      301 tgttcgactg cttcgacccc tcggcgttga ggatcgcggc ctccttgttg ccctcggcca
      361 ccgtgatcgc ggccgccttg gctccgtcgg cttcggtgac cacggcgcga cgggtgcgtt
      421 cggccgacat ctgacgggtc atggcctcct gcaccgccgc gggagggatg atctcgcgga
      481 tctcgacgtt ggtgaccttc acgccccagc gttcggtgac ctcgtcgagc ttgaggcgca
      541 gcagcgtgtt gatctcgtcg cgcttggcca gcatgtcgtc cagcgggatg tcaccgatca
      601 ctgctcgcaa cgtcgtggat gcgatgttca gcgccgcacc ggcgaagttg ccgacctgca
      661 ccaccgaggt ctgcgcgtcc atcaccttgt agaacatgac gaagtcgacg gcgatcggcg
      721 cattgtcctt ggtgatcgag tcctgcctcg ggatctccag gtagaactca cgcaggtcga
      781 cgaccgtcgc cttgtccacg aacgggatca gcaggatgag cccgggacct ttctggccca
      841 gcagccgtcc cagccggaac acgaccaagc gttggaactc gggcacgacc ttgatcgcct
      901 tggccaagaa cacgatcacc agcagcagga tcacgatcgc gccgatgagc gcgatgttga
      961 cgtccatcag gtgttcctcc ctccttcggc cggcgccgtg ccggccgtcg cgccctcatc
     1021 gttcgcggca tcgacggtga gcgcgagacc gtcgagccgg gtgaccacga cgcgcgcccc
     1081 ggcgggcagc gagcttccgt cggacgtgga ggccttccat tcctcggagg ccacgcgcac
     1141 gatccccttg ggatcgagac ctcgtccgat gaccacgccc tccttgccca ccacgacttc
     1201 gggtccctgg gcgggcgcca tgtgacgcat cttcagcacc ttggtcaaca ccaggaagaa
     1261 gaacgcaccg atgaagatgg cgacgatcgc gaggacggcc ggcgacaccg agaccccctg
     1321 ggaacggtcg tagaggaacc agccgccgag cacgaggcac gcgagtccgg cgaaccccca
     1381 gatgccgaaa ccgggagcgt ggagctccac gatcatcaac acgaccgcac cgatcaagag
     1441 cgcgatcccg accagctgga cgggcagcaa gccgaacgag acgacggaca ggatcagcag
     1501 gatcgtgccg atcgtgccgg agaacacgtg gcccgggacc aggagctcga gcacgatcaa
     1561 ggccaatccg acccagaaga agatgaaggc gaggttgggg tcgagcaggg agtgcaggaa
     1621 cccgatgaac ccgcccatcg gctggtcctc cagcacccag ccctgcgtgt cgagcgtcgt
     1681 cgtggtgccg tcgccgagcc ggacctgccg gccgtcgatg tcgacgagga gcgcttcggc
     1741 ggtgggagcg atcgcatcga tcacgtcggc agacaacgcc gtctcggccg tgatgctctc
     1801 ggccgatcgc acgaacgact cggccatgtc cgcgttgcgt ccgtacgtct cggcgaggct
     1861 cctgatgtag gcggccgcat cgttcacggc cttgtccgaa ccgatcgcac cgctgaggcc
     1921 gacgggggtg gccgctccga cgttggtgcc gggcgccatg gcggcgacgg gacaggagag
     1981 caacacgaac gccccggccg aggcggcccg agccccttgt ggtgagacgt agcagatcac
     2041 cggcaccttc gaattcaaga tcgcctgggt gatctggcgc atcgaggagt cgaggccccc
     2101 cggggtgtcg atctcgatca tcaccgcggg agccgggtcg tcaccgttgg ccacctcgat
     2161 cccgtgttcg atgtagtcgg ccaggaaggg gtcgaccacg ccctccagat ccagcagggg
     2221 gaccggttcg gccgatccgt cctgcgcgtc ggcggtcccc gcgagcacga acgcgccggt
     2281 cacggcgatc aggatcgccg tgaccatctg ctgcccagtt cgtctcgcgt ttcgcatgaa
     2341 cataaccctg gttcgtcaca tgtggagagt tggttcgaac cgagcgtaat cgggccgcga
     2401 acgctgtgca ggggttcctg cctggtcgat aatcgatcgg gcgtgtcgaa cggcgagcac
     2461 gccgcaagcc ccgaccagcg cgagctccgt atatcgaagg gacaccgggt gcaggctcgg
     2521 aacgacggtc cactcctccc acgagaggaa cagcaactga tggaagggct cctcgccggc
     2581 aacgacgacg ccatccgcac gctctattcg cggttcggtc gtcccgtcta ctcgctggga
     2641 cttcgtctgc tcggaacccg ggagtccgcc gaagaactca cccaggacgt cttcctcacc
     2701 gcgtggcgga aggcggcgcg gttcgatccg gcgcgcggac gcctgtccac ctggttgatg
     2761 acgatcgcgc acaacctcgc cgtcgaccgg ctccggcggg agaccggcgt gtcgcgtccg
     2821 acgctggtcc tcgtcgatga ggtgcccgac gccccgggcc tggatgaaga ggcggtcctg
     2881 atcgaacgcg actcggcgtt gcgcgcgctg gagtctctct caccggcgga gcgcctgctc
     2941 gtgggccggg catacttccg cgggcagacc gcccgcgaga tcgctgaggc cgacggcatc
     3001 cccctcggca cggtcaagac ccggctccgc tcggcgatga tcaaggtgcg caaggccaac
     3061 gagggcaagg acctgctgtg aactgcgtca ccgtgcggga tggactgacg gagcgtgcgc
     3121 tcggcaccct gcccgctcgc gagcgagcgc gcatcgaccg gcatctggtg tggtgcgcgg
     3181 cctgcaggaa ggaagccgcc gagatggaga gcgcggcggc cgtcttcgcc ttcggtgtcg
     3241 accagacgga acccgacccc gcgctcgagg cctcggtcat cgcctcgatc aaccacgcgt
     3301 cgggacgacg tgccccacac acagctccgc gacgaggccg actggcggtc gtcgcggtcc
     3361 tcgccgcgat gctcgccgtg agcggtctcg gctggggcgc cgtgatggcc ggccgggccg
     3421 cgcgctcgga cgaggcggca ctggtcacca cgctccgcca gcagagcgcg gccgagaagt
     3481 tctcccggat cctgtcctcg gccgagttcg ccgaccccga gggggaggtg ttcctcggca
     3541 cgctcggccc gacgaacgag ggtggaggtg gtggcagcgc gctcaccctg gtctcgccca
     3601 cgatcaccga catggccgtg gtgctcgtca acggcgtgcc gcccgcgcaa cgcaagggcg
     3661 tgccgttcac tgtgtggctc cgtggccccg acggcgcttc cctggcggcc ggccagatcg
     3721 atgcgctcga cgccgccggc tccgggatca tgtgggagga catcgatcgg gacctcggcg
     3781 tgttcgaccg cgtcgtcgtg cgcgatacgg gtggccacgc cgtgatgcgc gggatcatcg
     3841 ccacccgggc tccgctgtcc tcaccgagtc cctgatcgct cgccgacggc gctccgcgcg
     3901 ccgatgggta tcctcgggca catgcgattc gggctgagcc tgccgcacta cgggttctcc
     3961 ttgccggggt ccgcgcccat cacgttcggg gccatggccg cgtgggcgca ccgggcggag
     4021 gagctcgggt tcgactcggt gtcgatctcc gaccacttct tctattcgtt cggacgctac
     4081 ggtgccgatc cgcagccgat cgcttccctg gagcccatga cggcgctcgc cgggctggca
     4141 gcggtgacgg accgtgtccg tctcggcgcg ctcgtcctgt gcgcgccgtt ccgacatccg
     4201 tccaacctcg cgaagatggc cgccacgatc gatcagctct ccggcgggcg gctcgatctc
     4261 gggatcgggg cggggtggct cgaacaggaa ttctcggcgt tcgggtatcc gttcggcacc
     4321 gtcggtgaac ggttcgaggc gttggaagag acgctccagg tgctcgaagc gctgttctcc
     4381 ggagcgccgg cgaccttcga cggacccacg gtcacgctcc gtgatgcccg gctcctcccg
     4441 gcgccggcgc aggagccgcg gatcccggtg tgggtgggcg gcaagggcgg accgcggctg
     4501 ctgcgtctgg cggctcgtca cgcagacggc tggaacgccg tgtggcgctg ggcgccccag
     4561 gcctacgccg accgggtcgc ggccgccagg cagatctgcg agcaggaggg ccgtgacccc
     4621 gccacgttcc gtctctcggt ggggctctcc tgcctgctgg gtgaggacga ggccgccgcc
     4681 cgggcggcgt tcgagcgggg gcgggcctcg atgccgggcg atgcgatggc cgccgagacc
     4741 gacgcctcgt ggcgcgccga cacgctctcc ggcacgcctg agcaggcact ggagcggctc
     4801 cacgcgttcg gatcgctcgg cgtggaggag gtcgtgatcg ccccgtgggt gttgccgttc
     4861 gcggttcccg aaccggagca ggtggagctc ttcgccgagc gggtgatggg tccgttccgc
     4921 gcgggcgcgt gaggatggaa cctgcggcgg cgatcctcgc cgtgctggag gcggcggatg
     4981 ggccgctcca ctggacggtg atccaggatc gggcgctgcg tgccggctac ctcgatccgt
     5041 tcgagacccc ggatgtgcgt ggtgtggtgg ggcgcaccct gcgtgagctc gcgagcgcgg
     5101 ggacgatcgt cagggtggaa cagggcacgt accggtcggt ctagcggcga tcaggcaggc
     5161 ttcttgcggg gacgaccacg cttcttcggg gctgcggaga ccttcggctt ggcgctgccg
     5221 acgaccgcct tccgtcgtcc gggccgaggc ttgcgcgcac cggcgatcgc ctcgttgacg
     5281 tgcgtgctgc agaggtcctt cacgtagttc cctcgcgcga ccttgatcgt gatcgtctcg
     5341 gccgccggtc gtccgcaggt gtcgcagacc aagatcgtct tctccgccat gatggatccc
     5401 cttcgcgctc gccctggggg aaggatacgt cgccggcagg tccgagcggt aggtcggctc
     5461 agcgccgata ggcgatcggt gagaatcgtt cgagcttctc gaaccgatgc tcggcctcga
     5521 cccaccgcac ggtgcccgac ctcgaccgca tcaccatgga gtgcgtcgtg gcaccgtcgg
     5581 gccagtagcg aacccctcgg aggaggttcc cctcggtgat cccggtggcc gcgaagaaca
     5641 cgtcgtcgcc actgacgaga tcgtccgtgg acagcacggc gtcgacgtcg tagccgtcgt
     5701 cgacgagctg ctg
//
LOCUS       DATDBZ010000174         6165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_672015, whole genome shotgun sequence.
ACCESSION   DATDBZ010000174 DATDBZ010000000
VERSION     DATDBZ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672015"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..429
                     /locus_tag="VIX62_04280"
     CDS             202..429
                     /locus_tag="VIX62_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859480.1"
                     /translation="MTDWLTWWFILGGAVAVVLVAFLVALIRQVFLLGRTARRMQEEL
                     APITRDISSQAGRASSRAGSLKPPSRSGRTP"
     gene            501..962
                     /locus_tag="VIX62_04285"
     CDS             501..962
                     /locus_tag="VIX62_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY5859481.1"
                     /translation="MLNIGPPELLLIIVIALVVVGPQRLPDLGRTIGRGLREFRKVQD
                     EVRDMVDTGMGDDFKQTTAELRKTAAELRGATDVTSAFRNTPHKARQRAAEAVRAATT
                     PDDDEPAAEPAPEAPQSPDPAPPTSASVAPEIEPSEEPSSNGSQRPPEPEA"
     gene            962..1738
                     /gene="tatC"
                     /locus_tag="VIX62_04290"
     CDS             962..1738
                     /gene="tatC"
                     /locus_tag="VIX62_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY5859482.1"
                     /translation="MRVVSRFRRRSEEERAGSMTVMEHLEELRHRIIVCVIAVGVCGI
                     AAWFLYEPVKELLRDPFCQYITDNPQVRPPAGCDLVFSGPIDGFLVKLKMVGFLALAI
                     ALPIVLYQLWAFIVPGLTAKEKKWSIPFVVSSCVLFALGAVFAYVTLPKALDFLLGFA
                     GEGVVPLITFDRYMGFVVLVTLAFGISFLFPVLLVFLEAVGVLSTRKLREWRRWAILG
                     IAIFAAVITPSGDPYTMFAMMIPMYLFYEGSIIIGRMMKR"
     gene            1757..2494
                     /locus_tag="VIX62_04295"
     CDS             1757..2494
                     /locus_tag="VIX62_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859483.1"
                     /translation="MAIKGKGRSKSKPAARAPKRAPVPVPIPFTQRRWVQRTAFFILG
                     VLVVSGFVWLMNGLRKNDAESEATNVASSKRTAAQSYKSAVEDAVGTVAVVTPGVPPA
                     AFAEMGDTLQQMKQGTTPDDAGDVFATASKDAAAAAAAITKYDVGTKIDDKGFVATET
                     IAFTDSSQQIVQALRLYQHAADVGKVAAESSGPEAAQLTDVAQGLFDDAQTMMTQGWS
                     SFLSALSAGGVVDPPANAGIPGLPTGS"
     gene            2506..3420
                     /locus_tag="VIX62_04300"
     CDS             2506..3420
                     /locus_tag="VIX62_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY5859484.1"
                     /translation="MEISAPVVVVANPTAGRGKAGKLIGKADATLRQLRVEHQVLVTE
                     SPADMEATCRRVAGEGARIVAVIGGDGTVSCAANGLIGTDAALAVLPAGTGDDFAKTI
                     GAGTFDGAVRLLAAPKPVRIDAIRVTTDDGSRHFVNIAGAGFDSEVNETANAMTVNLG
                     GTGTYVAALMKTLSRFSPASYVLTLDDEPLDLDAMLVVVGNGISYGGGMKVLPDASLV
                     DGRLDVCIVEALSKAAFLRAFPKVFTGSHGSHPKVRLRTAASVTVRANRSMQVYADGE
                     HVGPLPATFEIVPGALTVVAGPQAKGIR"
     gene            3417..4166
                     /locus_tag="VIX62_04305"
     CDS             3417..4166
                     /locus_tag="VIX62_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5859485.1"
                     /translation="MTESADGVLLIHAFPLDASMWERQVRTLGDAGWSVVAPSLPGFG
                     DTDGVGPTMTMGAAADRCLQAADAAGIERFVVCGLSMGGYVAFELWRMARQRIAGLVL
                     ANTRSGADAPEAAQGRRALADRLDTEGNGFLVEQPPPLLSDQAPAELWEQVRGAIRAQ
                     PAASIGAAARGMAERIDSTPDLGAVDVPMLIVTSSLDTLIPAAVSAEMRDHHRDAGLV
                     EIPGAGHLSNLEAPEAFDRTLLAFLATFDRA"
     gene            4219..>6165
                     /locus_tag="VIX62_04310"
     CDS             4219..>6165
                     /locus_tag="VIX62_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5859486.1"
                     /translation="MTTPQAFAAQFPFALDTFQLEAAAAVHEGLSVLVAAPTGSGKTV
                     VAEFAIERALALGGKCFYTTPLKALSNQKFGDLVAKHGAQTVGLLTGDNTVNPDAPVV
                     VMTTEVLRNMLYERSDALSSLVSVVMDEVHYLQDPYRGAVWEEVLIHLPLSVNVVCLS
                     ATISNAEEFGEWIGTLRGETRVIIEEQRPVPLEHHYLVGHGMHPMHVEQDGVPLPNPY
                     VVSLDQRELRTKTYYRRGSGTPQQQRISRPREGHRRVYVPKREEVVEVLDEEGMLPAI
                     YFVFSRAGCDKSVRWLRESGISLTSRAEADHIRERAETRAAWVDEADLVTLGFYEFLD
                     GLMAGIAAHHAGMLPVFKETVEELFEAGLVKVVFATETLSLGINMPAKTVVIEDLWKF
                     QGERHELLTPGEYTQLTGRAGRRGIDEVGHAVVVYQRQVPFERVATLASTRTYNLTSS
                     FRPSYNMAVNLVRNYTPEQAHHLLNSSFAQFLADRGVVALERTRQQDHEAIEGYRRNM
                     ACHLGDFDEYWGLLAQANELREQDRLGRDKSRKDAIKGAVASLRPGEVIQVPSSKRGG
                     LAVVVSSRDGKPSVLGQDRRSFRLQVSDFREPPQVLTRIALPRSGSTRSARYRRDVAA
                     SLVSLRVKPSKDSSRRPDPKVEREA"
BASE COUNT          973 a         2072 c         2123 g          997 t
ORIGIN      
        1 cgacgaggag gtcgcggagg atcctcgcgg gcctctgcca cagggccccc cattctcgat
       61 cgacgacgtc atcgagttcc actacctgct ggaggacgat cggtacatcg ccgaattcgt
      121 cgcctccagc ggcggcaact gagcaccacc cgccgcttcc tgcgccccgt tccgccctcc
      181 ggcctcgtgt agcatcgcga cgtgaccgat tggctgacgt ggtggttcat ccttggcggc
      241 gccgtcgcgg tggtgctggt ggcgttcctc gtggcgttga tccgacaggt gttcctgctc
      301 ggccggacgg ctcggcggat gcaggaggaa ctcgctccca tcacgcggga catctcctcc
      361 caggcggggc gggcgagttc gcgagcaggg tcgctgaagc ctccgtcacg atccggacgt
      421 acgccctgac ctgcaaggac gccttcgtcg gggcaccgcg acggtaggat aagagccctg
      481 accccgagga tccgacgccc atgctgaaca tcggtccgcc tgagctcctg ctcatcatcg
      541 tcatcgcttt ggtcgtcgtg ggcccgcagc gcctgcccga cctcgggcga acgatcgggc
      601 ggggcctccg ggagttccgc aaggtccagg acgaggtccg cgacatggtc gatacgggca
      661 tgggcgatga cttcaaacag accacagccg agctgcgcaa gaccgcggcc gagttgcgtg
      721 gcgccaccga cgtgacctcg gcgttccgca acacgcccca caaggcccga caacgcgccg
      781 ccgaggccgt gcgcgccgcc accacgcccg acgacgacga gcccgctgcg gagccggccc
      841 ccgaggcacc gcagagccct gatccggcgc cgccgacctc cgcctcggtc gcacccgaga
      901 tcgaaccttc ggaagagccc agctccaacg gatctcaacg gcctccggag cctgaggcct
      961 gatgcgcgtg gtctccaggt tccgacgccg ctccgaggag gagcgggcgg gcagcatgac
     1021 cgtgatggag cacctcgaag aactccgtca tcggatcatc gtgtgcgtca tcgccgtcgg
     1081 ggtgtgtggg atcgcggctt ggttcctgta cgagcccgtg aaggaactcc tgagagatcc
     1141 gttctgtcag tacatcaccg acaaccctca ggtccgcccg cccgcgggtt gcgacctggt
     1201 attcagcggc ccgatcgacg gattcttggt gaagctgaag atggtgggct tcctggccct
     1261 cgccatcgct ctgccgatcg tgctctacca gctctgggcc ttcatcgtgc ccgggctcac
     1321 cgccaaggag aagaagtggt cgatcccgtt cgtcgtgtct tcctgcgtct tgttcgccct
     1381 gggcgcggtg ttcgcctatg tcaccttgcc caaggccctg gacttccttc tgggattcgc
     1441 cggagagggc gtggtgccgc tcatcacgtt cgaccgatac atgggcttcg tcgtgctggt
     1501 caccctcgcg ttcgggatct cgttcctgtt cccggtgctg ttggtgttcc tcgaggcggt
     1561 cggcgtcctg tcgacccgca agctgcgtga atggcggcga tgggcgatcc tcgggatcgc
     1621 catcttcgcg gccgtgatca ctccttccgg tgacccctac acgatgttcg cgatgatgat
     1681 cccgatgtat ctgttctacg agggctctat catcatcggt cgcatgatga agcgctgagc
     1741 cgacgaagga gcgtccgtgg cgatcaaggg caagggcagg tccaagagca agccggcagc
     1801 acgcgccccg aagcgcgccc cggtaccggt gcccatcccc ttcacgcaac gccgatgggt
     1861 gcagcgaacg gcgttcttca tcctcggtgt gctggtcgtc agcgggttcg tgtggctgat
     1921 gaatggcctg cggaagaacg acgcggagtc ggaggcgacc aacgtggcgt cctccaagcg
     1981 aacggccgcc cagtcctaca agtccgccgt ggaggacgcg gtgggcacgg tcgccgtggt
     2041 gacccccggg gtcccgccgg cggcattcgc cgagatgggt gacaccctgc agcagatgaa
     2101 gcagggcacc acgcccgatg acgccggcga cgtcttcgcc acggcctcga aggacgccgc
     2161 ggccgcggcg gccgcgatca ccaagtacga cgtcggcacg aagatcgacg acaagggctt
     2221 cgtcgccacc gagacgatcg ccttcaccga ctcgagccag cagatcgtgc aagcgctccg
     2281 gctctatcag cacgcggcag acgtggggaa ggtggcggcc gagtcatcgg ggcctgaggc
     2341 cgcccaactc accgacgtcg cgcagggcct cttcgacgat gcgcagacga tgatgaccca
     2401 ggggtggtcg tcgttcctgt ccgcgctctc ggctggtggg gtcgtggatc cgccggcgaa
     2461 cgccgggatc cctggcctcc cgaccggcag ctagccggag gtcgcgtgga gatcagcgct
     2521 cccgtcgtgg tggtggccaa ccccaccgcg gggcggggca aggcgggcaa gctcatcggc
     2581 aaggcggacg cgacccttcg gcagctccgg gtggaacatc aggtcctggt cacggaatcg
     2641 cccgccgaca tggaagccac ctgccggcgc gtcgcgggcg agggtgccag gatcgtcgcc
     2701 gtgatcggcg gggacggcac cgtctcatgc gccgccaacg gcctcatcgg gaccgacgcc
     2761 gccctcgccg tcctgccggc cgggaccggt gacgacttcg ccaagacgat cggcgccggc
     2821 accttcgatg gggcggttcg gctgctcgcc gccccgaagc ccgtacggat cgatgccatc
     2881 cgtgtcacca cggacgacgg gagccggcat ttcgtcaaca tcgctggggc ggggttcgac
     2941 tcggaggtca acgagaccgc gaacgccatg acggtcaacc tcggcgggac gggcacctac
     3001 gtcgcggcgc tgatgaagac cctgtcgagg ttcagtcctg caagctacgt gctgacgctg
     3061 gacgatgaac cgctggacct ggacgcgatg ctggtggtgg tcggcaacgg catctcctac
     3121 ggtggtggga tgaaggtgct gcccgacgcc tcgctcgtgg atggccgcct cgacgtctgc
     3181 atcgtcgaag cgctgtcgaa ggccgccttc ctgcgagcgt tcccgaaggt gttcaccggg
     3241 tcgcacgggt cccaccccaa agtccgcctg aggaccgcgg cctccgtcac cgtgcgagcc
     3301 aaccgttcga tgcaggtgta tgccgacggg gaacacgtcg gccctctgcc ggccacgttc
     3361 gagatcgttc ccggcgccct caccgtggtg gccgggcctc aagcgaaagg gatccgatga
     3421 ccgaatctgc cgatggtgtg ctgttgatcc acgcgttccc gttggatgca agcatgtggg
     3481 agcgacaggt gaggaccctc ggggacgctg gatggtcggt ggtggcgccg tcgctgcccg
     3541 ggttcggcga caccgacggc gtcgggccca cgatgacgat gggcgccgcc gccgaccgtt
     3601 gcctgcaggc cgccgacgcc gcggggatcg agcggttcgt ggtgtgtgga ctgtcgatgg
     3661 gcggctacgt ggcgttcgaa ctctggcgga tggcgcgcca gcggatcgcc gggttggtgc
     3721 tcgcgaacac gcgatcgggt gccgacgcgc cggaggccgc gcaaggccga cgcgcgctgg
     3781 ccgaccgcct ggacaccgag gggaacggct tcctggtgga gcaaccgccg ccgctcctca
     3841 gcgaccaggc ccccgccgag ttgtgggagc aggtccgagg cgcgatccgg gcgcaacccg
     3901 cggcctcgat cggagccgcc gcccgcggca tggccgagcg gatcgactcg acgcccgacc
     3961 tcggagcggt cgacgttccc atgctgatcg tgacctcctc gctcgacacc ttgatcccgg
     4021 ccgcggtctc ggccgagatg cgcgaccatc accgcgatgc cggcctggtg gagatccccg
     4081 gtgccgggca tctgtccaac ctggaagccc cggaggcctt cgaccgaacg ctcctggcgt
     4141 tcctcgcgac cttcgatcgc gcctgaggat ctcgcgcgca gcgccggggg cggaaccgct
     4201 cgcccgtaga ctgaccgggt gaccaccccc caggcgttcg ccgcccagtt cccgttcgcg
     4261 ctggatacgt tccagttgga ggccgcagca gcggtccatg aagggttgtc cgtcttggtg
     4321 gcagccccca cggggagcgg caagaccgtg gtggccgagt tcgcgatcga acgggcgctc
     4381 gccctcggcg gcaagtgctt ctacacgacg cccctgaagg ctctgtccaa ccagaagttc
     4441 ggcgacctgg tggccaagca cggcgcgcag acggtcgggc tcctgaccgg cgacaacacc
     4501 gtcaatccgg acgcgccggt ggtggtgatg accaccgagg tgctgcgcaa catgctctac
     4561 gagcgcagcg acgccctctc atcactggtg agcgtcgtga tggacgaggt gcactacctg
     4621 caggacccct accgcggcgc cgtgtgggag gaggtcctga tccacctccc gctgtcggtc
     4681 aacgtggtct gcctgtcggc cacgatctcg aacgcggagg agttcggcga gtggatcggc
     4741 acgctgcgag gtgagacccg ggtcatcatc gaggagcagc gtcccgtgcc cttggagcat
     4801 cactacctcg tcgggcacgg catgcatccc atgcacgtgg aacaggacgg cgtgccgttg
     4861 cccaacccct acgtcgtctc gctcgatcag cgtgagctgc gcacgaagac ctactaccgc
     4921 cgggggagcg gcacgccgca gcagcagcgg atctcccggc cgcgcgaggg tcaccgacgg
     4981 gtgtacgtcc ccaagcgcga ggaggtcgtc gaggtcctcg acgaggaagg catgctgccg
     5041 gcgatctact tcgtgttcag tcgcgcaggc tgcgacaaga gcgtgcggtg gctccgcgag
     5101 tcgggcatct cgctcacgag ccgcgcagag gccgaccaca tcagggagcg ggcggagacg
     5161 cgggcggcct gggtcgacga ggctgatctg gtcacactgg ggttctacga gttcctcgac
     5221 ggtctgatgg cggggatcgc ggcccaccac gccggcatgc tcccggtctt caaagagacg
     5281 gtggaggagt tgttcgaggc cggcttggtg aaggtggtgt tcgccaccga gacgctgtcg
     5341 ttgggcatca acatgccggc caagacggtg gtgatcgagg acctgtggaa gttccaaggg
     5401 gagcgacacg agctgctgac cccgggggag tacacgcagc tcaccggacg ggcgggccgg
     5461 cgaggcatcg acgaggtggg ccatgcggtg gtcgtgtatc agcgtcaggt gccgttcgag
     5521 cgggtcgcca cgctcgcgtc gacccggacc tacaacctga cgtcgtcgtt ccggccgtcc
     5581 tacaacatgg cggtgaacct ggtccgcaac tacacgccgg agcaggctca ccatctgttg
     5641 aactcctcct tcgcgcaatt cctggccgac cgtggggtcg tggcgctcga gcgcacgcgg
     5701 cagcaggacc acgaggcgat cgagggctac cggcgcaaca tggcgtgtca cctcggtgac
     5761 ttcgacgagt actggggcct gctcgcccag gcgaacgagc tccgcgagca ggaccggcta
     5821 ggccgggaca agtcccggaa ggacgcgatc aagggcgcgg tggcctcgtt gcgaccaggc
     5881 gaggtcatcc aggtgccatc ctccaagcga ggaggcctgg cggtcgtcgt ctcctcacgg
     5941 gacggcaagc cgagcgtgct gggacaggac cgccgttcgt tccgattgca ggtgagcgac
     6001 ttccgcgagc ctccgcaggt gttgacgagg atcgccttgc cgcggtcggg gagcacgcgc
     6061 agtgcccgct accgtcgcga cgtcgcggcc tccctcgtgt ccctccgggt caagccctcc
     6121 aaggactcct cccggaggcc agatccgaag gttgaacgcg aggcc
//
LOCUS       DATDBZ010000175         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1060715, whole genome shotgun sequence.
ACCESSION   DATDBZ010000175 DATDBZ010000000
VERSION     DATDBZ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060715"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VIX62_04315"
     CDS             complement(<1..341)
                     /locus_tag="VIX62_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5859487.1"
                     /translation="MDVGDAGPRVIGIDVGGTKIAGGVVDLGAGSVHERESMPTHAER
                     GGTAVLDDVRALASRLVGGARHGGDPVRLGIAVCELVEPSGAITSAQTLDWLDRDVVG
                     AVADVASATIAS"
     gene            complement(355..1221)
                     /locus_tag="VIX62_04320"
     CDS             complement(355..1221)
                     /locus_tag="VIX62_04320"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5859488.1"
                     /translation="MTPVAPAGSPRPSFEEATHVPYDEATRHLWGDDEAGRVADWIYV
                     SSDKIHHLVFALPPGGMFRHSEGFRTVFAADEVLYVLSGTMVFADPEHGEVRRVEAGQ
                     GVFFRRDTWHHAMSFGDDQLRVLEFFAPPPATGASSAYARTKDFLSDPRYQDHRWLGR
                     WPMAADERAAASRLQVLGSGETLDSLEGDGAFVQTFASTEHLTVEKITIRPGGHSDTR
                     RHGGDAAMHALHGTLNVLLTEGPDSHGQRWFEVHPDDGFFVPEGTSYELRNVTGDEVQ
                     VLTGVAPSFTTD"
     gene            complement(1318..2076)
                     /locus_tag="VIX62_04325"
     CDS             complement(1318..2076)
                     /locus_tag="VIX62_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5859489.1"
                     /translation="MSASVTLALDPARKDIPLYQQVQHAIAEHIAAGRLAPGQQLPSE
                     RRLCERFDISRVTARRALAALVDEGLIQASPGKGWFVSDGHLSEPPNALLSFTALARA
                     RGLEPSARVLRHEVRDATMDEAESLQVAPGAEVLDLERVRMLDDLPVAVDHCLIPLGQ
                     CPELAEADFTVDSVYEILRTKARIAPTYADCTLEATAAGASIAPLLDLEPGSPVLVAR
                     QTTFDQSDRPVESSRITYRGDRYRFRTKLTAETQ"
BASE COUNT          386 a          759 c          765 g          364 t
ORIGIN      
        1 gaggcgatcg tcgccgaggc gacatcggcc accgccccca cgacgtcccg atcgagccag
       61 tcgagggtct gtgcactcgt gatggcgccc gatggctcga ccaactcaca gacggcgatg
      121 ccgagtcgca cggggtcgcc accgtggcgc gcgccgccga cgagccgtga ggccaatgca
      181 cggacatcgt ccaggaccgc ggtgccacca cgctccgcat gggtcggcat cgactcacgc
      241 tcgtgcaccg agccagcgcc cagatccacg acgccgcctg cgatcttggt gccaccgacg
      301 tcgatcccga tgacacgagg acctgcatcc cccacgtcca tcgacccccg cgtgttagtc
      361 cgtcgtgaac gagggcgcga cgccggtcag gacctgcacc tcgtcacccg tcacgttccg
      421 tagctcgtag ctcgttccct cgggaacgaa gaagccgtcg tccgggtgca cctcgaacca
      481 ccgctgcccg tgtgaatcgg gaccctcggt gagcagcacg ttgagggtgc cgtgcagggc
      541 gtgcatcgcg gcgtcgccac cgtgccgtcg ggtgtcggag tgccccccgg gacggatcgt
      601 gatcttctcg acggtgaggt gctccgtgct ggcgaacgtc tgcacgaacg cgccatcgcc
      661 ttcgaggctg tccagcgtct cacctgatcc cagcacctgc agccgggagg cggcggcccg
      721 ctcgtcagca gccatcggcc agcgtccgag ccagcggtgg tcctggtagc gaggatcgct
      781 caggaagtct ttcgttcgtg cgtacgcgct ggaggcaccg gtcgctggag ggggggcgaa
      841 gaactcgagg acccggagtt ggtcgtcacc gaagctcatc gcgtgatgcc aggtatcacg
      901 acggaagaag acgccctggc ccgcctccac acggcgaacc tcaccgtgtt cggggtcggc
      961 gaacaccatg gtgccggaca agacgtagag cacctcatcg gcggcgaaca ccgttcggaa
     1021 gccttcggag tggcggaaca tgccacctgg cgggagcgcg aacacgaggt gatggatctt
     1081 gtcgctcgac acgtagatcc agtcggcgac gcgacctgcc tcgtcgtcgc cccacaggtg
     1141 ccgcgtggct tcgtcgtacg ggacgtgcgt cgcttcctcg aaactgggac ggggagagcc
     1201 ggccggcgca accggggtca tgggtctcgg aacgtcgcgt tggaggcctt cactcatatg
     1261 ccgaccatca tactcaggaa ccgatttctg ggaagaccag tagtaagacc agttgggtca
     1321 ctgggtttcg gcggtgagct tggttcggaa ccggtaccgg tcccctcggt aggtgatccg
     1381 agagctctcc acgggccgat cgctctgatc gaaggtggtc tgacgggcca ccaaaacggg
     1441 tgagccaggt tcgaggtcga gcaacggcgc gatcgatgct cctgccgccg tggcctccag
     1501 tgtgcagtcg gcgtaggtgg gcgcgatgcg ggccttcgtg cgcaggatct cgtagacgga
     1561 atccacggtg aagtccgcct cggcgagctc ggggcactgc cccagcggga tcaggcagtg
     1621 atcgaccgcc accggcaggt catcgagcat gcgaacccgc tccaggtcca agacctcagc
     1681 tcccggtgcg acctgcaacg attcagcctc atccatcgtg gcgtcgcgca cttcgtggcg
     1741 gagcacccga gcgctcggct caaggccacg agctcgagca agagcggtga aactgagcag
     1801 cgcgttcgga ggttcgctga ggtgcccgtc cgacacgaac caccccttgc ccggcgaggc
     1861 ttggatcagg ccctcgtcga ccagcgccgc gagggcacga cgagcggtca cacggctgat
     1921 gtcgaagcgc tcgcagaggc gtcgttcgga gggcagctgt tgccccggcg ccaaacggcc
     1981 ggcggcgatg tgctcagcga tcgcgtgctg cacctgctgg tagaggggga tgtccttgcg
     2041 cgccggatcg agcgcgaggg taaccgacgc cgacatggct ccatcctccg cgccacggcg
     2101 ccaaggtgtc aaggactggt cttatgctgg tctcatgacc ggtttcacga tctcggtcgt
     2161 cgacgggcct accgaagcgg ggttcgcagc cgccgacgtg atcgcggcct cgatcgcgac
     2221 cacaccccac gcagccgtgg tcatcgcgac gggtcgcacg cccgcgatca cgta
//
LOCUS       DATDBZ010000176         5421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2720564, whole genome shotgun sequence.
ACCESSION   DATDBZ010000176 DATDBZ010000000
VERSION     DATDBZ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2720564"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VIX62_04330"
     CDS             <1..822
                     /locus_tag="VIX62_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5859490.1"
                     /translation="IAGEGPVVVIDPGPDDPAHLAEVANTAAATGPVAAVAVTHDHPD
                     HAPGAVAFAATVGAPLFAFRLAGAEHVRDGQRLPVPEMDIVAIHTPGHTSDHVAFHVP
                     RDRALFTGDAVLGRGTSFIDPPDGDLAQYLRSLAVMQDLKPRTIYPGHGPVVLDAERT
                     LQEYVDHRAEREAQVVAAVAGDGATVKAMVATIYADYPAEVHELAARSVLAHLLKLEA
                     EGRVQKRGRGKDPVWLAAEPRSCERCGRPVKGRGRYCPTCSLVILQEGSTAEPGV"
     gene            complement(837..1421)
                     /locus_tag="VIX62_04335"
     CDS             complement(837..1421)
                     /locus_tag="VIX62_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5859491.1"
                     /translation="MAPTAARRIGTIEDDLPVPAGRVILRQGEPCSGVSVVMHGALLE
                     SAVDDDGRVLGLDVLGPSDPVGGPHGSLAPADVRALSAVRLRPVAPDRAGWLVDGRRQ
                     RLADLAHQLAWLDVTDRVSLRLEDLARRFGRPAPGGTLVRLALTQEDLAALCGTSRES
                     ANRALRALRDDGRVLVVGRGRFLIPALLAVPGAR"
     gene            1587..2009
                     /locus_tag="VIX62_04340"
     CDS             1587..2009
                     /locus_tag="VIX62_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5859492.1"
                     /translation="MAKPKLDNVLAGVPLFEGLSKRHLKKLGSLAEMADFMEGASVVK
                     EGQPGESFFVAITGQAKVTVKGRTVHRILPGDHFGEISLIDGGDRTATVVTETPMTLL
                     MIDRKPFLKMLADDPDVAVSLLEGLARMIRRVDRSLAR"
     gene            2021..2419
                     /locus_tag="VIX62_04345"
     CDS             2021..2419
                     /locus_tag="VIX62_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859493.1"
                     /translation="MKVEFTPPTPPPPADRPDAKVEPAPVVATARWVDGSVLVEADDP
                     EMRRVVERAFRRTPVVTDDASYRRLGTHGEVQVQPGDLEWFRAVAYERVPAELGLVAR
                     LVPGVTEGGFDPAAGYRRFDESIERLESGT"
     gene            complement(2421..>5421)
                     /locus_tag="VIX62_04350"
     CDS             complement(2421..>5421)
                     /locus_tag="VIX62_04350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY5859494.1"
                     /translation="ENLRSRVRRALAGVDLPEGEEPEIVNYHAMAAQVLDRYGILAGI
                     EPGQRVLSQAQRVELAGRVLDEMTFDHVKTEWQPSVVDKILNLDAQAQNHLVEPDAIV
                     EFCTSRLEALKGNRSDRAYYSALERIELAKASAVFRRLKRDLGVIDFGDQIELAMRIA
                     TTHPEVGQEYRDRFGAVLLDEYQDTNVAQARLMEALFGGGHPVTAVGDPDQNIYAWRG
                     AALSNLLDFPTVFSCSDGSPAARLPLFTNFRSGARILSAADTVIGPLPANQRPDPEKR
                     LVPFPPNGEGEVTVASHRDELTEAQWVADRIVALRDDGARWSEVAVLCRTSRLFFLLQ
                     QAFAERDIPVEVVGLAGLLRLPEVVEVLAYARAANDAMASVALARILMGPRYRVGFKD
                     LALVARWAKGKNYQWREEGGDDEDTPFLFAEALEHLDEVEGLSEDGIARLEEFRDELA
                     DLRTQARRPVAEFLGEVIRRIGIIDELDADVDRVVAAQRGRNLAAFLEQVHGFEPVEG
                     ELTLRAFLDYVDAVEALDKEEWEPVQPTDEDAVKVMTIHQAKGLEFDHVFVPGVATGL
                     LPSRRIQQNPAERGYSLDFELRGDASILPQFDGVLSHFKRDLQQQEIIEERRTMYVAL
                     TRARRSLWVSASTWYGENVNAKGPSQFFAELAAWGTDSGEATVTVASGEPADDEQNPM
                     LGQRLNLVRDWPGPARPDDTDALFTSGWRAAAVEAVTDGAVQAALVDSLAGEDRGTFE
                     RAAAEHRQLASHLRDREAVEGGPGVERVPQTVGASAVITYAQCPKRFYWSSVRPLPRF
                     SGPAARIGTEIHAWIERRARGQGQLLEMDDRPDLTDEELAGDPGRVERLRDAFMTSRF
                     ADVSPLFAERAFLLRLGDSSVGGRIDAIFGTVDGPWEVVDWKTGRKPSAADATAGLQL
                     DIYGLAAVEIWGKASADVTLTYFYLASGEEVTRPMDDPDVVRQRVLASLDAIAAGEFD
                     PTPGSWCRHCDFRSFCDAGKAWLAAQP"
BASE COUNT          816 a         1889 c         1930 g          786 t
ORIGIN      
        1 atcgcgggcg agggccccgt cgtggtgatc gaccccggcc cggatgaccc ggcgcacctc
       61 gcggaggtcg cgaacaccgc ggccgcaacg ggcccggtgg cggcggtggc ggtgacgcac
      121 gatcaccccg accatgctcc gggtgcggtg gcgttcgctg cgacggtggg tgcgccgctg
      181 ttcgccttcc gcctcgccgg cgccgagcac gtgcgggacg gccagcggct gccggtgccg
      241 gagatggaca tcgtggcgat ccacaccccc gggcacacga gcgaccacgt ggccttccac
      301 gtgccccgcg accgcgcgtt gttcacgggc gacgcggtgc tgggccgggg aacgagcttc
      361 atcgatccac cggacgggga tctggcgcag tacctgcggt ccctggccgt gatgcaggac
      421 ctgaagccgc gaacgatcta tccggggcac ggccccgtcg tgctggacgc cgagcgcacg
      481 ctgcaggagt acgtggacca ccgggcggag cgggaggcgc aggtggtggc ggcggtggca
      541 ggcgacggcg ccaccgtcaa ggcgatggtg gccacgatct acgccgacta cccggccgag
      601 gtgcacgagc tggcggctcg ctccgtgctc gcccatctgc tgaagctgga ggccgaaggc
      661 cgcgtgcaga agcggggccg gggcaaggac cccgtgtggt tggcggccga gccgcgttcc
      721 tgcgaacgat gcggcaggcc ggtcaagggc cgcggtcgct actgcccaac gtgcagcttg
      781 gtgatcctgc aggagggttc gaccgccgag ccgggcgtct gagccggagg ggggcctcat
      841 cgggctcccg gcacggcaag cagcgcgggg atcaggaagc gcccccgccc cacgaccagg
      901 acgcggccgt cgtcacggag ggcccgcaac gctcggttcg ccgactcacg cgatgtaccg
      961 cacagcgctg ccagatcctc ctgggtgagc gcgaggcgca ccaacgtgcc gcccggagcc
     1021 gggcgtccga atcgccgcgc caggtcttcg aggcggaggg acacgcgatc ggtgacgtcg
     1081 agccacgcca gctggtgggc gagatcggcg agtcgttggc gacgaccgtc cacgagccac
     1141 ccggcacggt cgggtgccac cgggcggagc cggacggcgc tgagcgctcg cacgtcggcg
     1201 ggggcgagcg aaccgtgcgg cccgccgacg gggtcgctcg gtcccaggac gtcgagtccc
     1261 agcacccgac cgtcgtcgtc gaccgccgat tccagcaggg cgccgtgcat cacgacggac
     1321 acccccgaac agggctcgcc ctgccgcagg atgacccggc ctgccggaac gggcaggtcg
     1381 tcttcgatgg tgccgatgcg gcgagccgcg gtgggtgcca cgacaccgtc tcccttccgg
     1441 gggaggtccg gtggagcgcg aaccatatcc gccggcgccg acatgcgccg tctcgccggt
     1501 gtgacgcagc gtcctccgca gctgccacac gggctaggct gtggacctca ggcccgaccg
     1561 aagaacgaac gcaccgagga ggcatcatgg cgaagcccaa gctcgacaac gtgctggccg
     1621 gggtgcccct gttcgagggg ctctcgaaac gacacctgaa gaagctgggc tcgctcgcgg
     1681 agatggcgga cttcatggaa ggcgcctcgg tggtgaagga ggggcagccg ggcgagagct
     1741 tcttcgtggc gatcaccggg caggcgaagg tcaccgtgaa gggacggacc gtccatcgca
     1801 tccttccggg cgatcatttc ggcgagatct cgctgatcga cggcggggat cgcaccgcca
     1861 ccgtcgtcac cgaaacgccg atgaccctgc tcatgatcga ccgcaaaccc ttcctgaaga
     1921 tgctcgcgga cgaccccgac gtcgcggtct cgttgctgga gggtctggcc cgcatgatcc
     1981 gccgggtcga ccgttcactc gcccggtagc ctgctgcctc atgaaggtcg agttcacccc
     2041 gcccacgccg ccaccacctg cagatcgacc ggacgccaaa gtcgagccgg ctcccgtcgt
     2101 ggccaccgcg aggtgggtcg atggttcggt cctcgtcgag gccgacgatc cggagatgcg
     2161 cagagtggtg gagcgagcgt tccggcggac tcccgtggtc accgacgacg cgtcctatcg
     2221 ccggctggga acccacggcg aggtccaggt gcagccgggg gatctcgagt ggttccgggc
     2281 cgtggcctac gagcgggtac ccgcggaact cgggttggtg gcccggctgg taccgggcgt
     2341 caccgaaggg ggcttcgacc ccgcagccgg ctaccgccgg ttcgacgagt cgatcgaacg
     2401 cctggagtcg gggacctagc tcacggctga gccgcgagcc acgccttccc cgcgtcgcag
     2461 aagctccgga aatcacagtg ccggcaccac gagccggggg tgggatcgaa ctcgcccgcc
     2521 gcgatggcat cgagcgaagc cagcacccgc tgccgcacca cgtcggggtc gtccatgggg
     2581 cgggtgacct cttcaccgct ggcgaggtag aagtacgtga gggtgacgtc ggccgacgcc
     2641 ttgccccaga tctccacggc ggccaggccg tagatgtcga gctgcaggcc ggcggtggca
     2701 tcggccgccg agggtttgcg acccgtcttc cagtcgacca cctcccacgg cccgtcgacg
     2761 gtgccgaaga tcgcgtcgat gcgtccgccg acggacgagt cgcccagccg aagcaggaac
     2821 gcgcgctcgg cgaacagcgg cgagacgtcg gcgaaccggc tggtcatgaa ggcatcgcgg
     2881 agacgctcca cgcgtcccgg gtcacccgcc aattcctcgt cggtgaggtc gggccggtcg
     2941 tccatctcca gcagctgacc ctggccacgg gcgcggcgtt cgatccaggc gtggatctcg
     3001 gtgccgatac gcgccgccgg gccactgaac cgcggaagcg gacgaacgct cgaccagtag
     3061 aagcgcttgg ggcactgcgc gtaggtgatg acggcgctgg cgcccaccgt ctggggtacg
     3121 cgctcgacgc ccgggccacc ttcgacggcc tcgcgatcgc gcaggtgcga ggcgagctga
     3181 cgatgctcgg cggcggcgcg ctcgaacgtc ccgcggtcct cgcccgcgag tgagtccaca
     3241 agcgcggcct gcaccgcacc gtcggtgacc gcctcgacgg cggcagcacg ccatcccgac
     3301 gtgaacagtg cgtcggtgtc gtcgggccgc gccggaccgg gccagtcgcg aaccagattc
     3361 agccgttggc ccagcatggg gttctgctcg tcgtcggcgg gttcgcccga ggccaccgtc
     3421 accgtggcct cgccgctgtc ggtcccccac gccgcgagct cggcgaagaa ctggctgggt
     3481 cccttcgcgt tgacgttctc gccgtaccag gtgctggcgc tgacccagag cgagcgtcgc
     3541 gcccgggtga gcgcgacgta catcgtgcgc cgttcctcga tgatctcctg ctgttggagg
     3601 tcccgcttga agtgggagag cacgccgtcg aactgcggca ggatcgaagc gtcaccgcgc
     3661 agctcgaagt cgagcgagta gccgcgctcg gcggggttct gctggatgcg ccgggagggc
     3721 agcagccccg tggccacccc cggcacgaat acgtgatcga actccaagcc tttggcctgg
     3781 tggatcgtca tgaccttgac ggcgtcctcg tcggtgggct ggacgggttc ccactcctcc
     3841 ttgtccagcg cctcgaccgc atcgacgtag tcgaggaacg cgcgcagcgt gagttcgccc
     3901 tcgacgggct cgaagccgtg cacctgctcc aggaacgccg cgaggttgcg tccgcgctgg
     3961 gccgccacca cgcggtcgac gtcggcgtcg agctcgtcga tgatgccgat gcggcggatc
     4021 acctcgccca ggaactccgc caccggccgc ctcgcctgcg tgcggaggtc ggcgagttcg
     4081 tcccggaact cctccagcct ggcgatgccg tcctcggaca agccctcgac ctcgtcgaga
     4141 tgctccaagg cctcggcgaa caggaacggc gtgtcctcgt cgtcgccacc ttcctctcgc
     4201 cactggtagt tcttaccctt ggcccaccgg gcgaccagcg cgaggtcctt gaaccccacc
     4261 cggtagcggg gacccatgag gatgcgtgcc agggccacgc tggccatcgc gtcgttggcg
     4321 gcgcgggcgt acgccagcac ctccaccact tcgggcaggc gcagcagccc ggccaggccc
     4381 acgacctcga ccgggatgtc ccgttcggcg aaggcctgct gcagcaggaa gaacagccgc
     4441 gaggtgcggc acagcacggc gacctccgac catcgggcgc cgtcgtcgcg cagcgccacg
     4501 atgcgatcgg ccacccactg cgcctcggtg agctcgtcgc ggtgcgaggc cacggtgacc
     4561 tcgccctcgc cgttcggcgg gaacggcacg agccgcttct cggggtcggg acgttggttc
     4621 gccggcagtg gtccgatgac ggtgtcggcc gcgctgagga tgcgggcgcc gctgcggaag
     4681 ttggtgaaca gcggaagccg ggccgccggc gacccatcgc tgcacgagaa caccgtgggg
     4741 aagtccagga ggttcgacaa ggcggcgcca cgccacgcgt agatgttctg gtcggggtca
     4801 cccacggccg tgacgggatg accaccgccg aacaacgctt ccatcagccg ggcctgcgcc
     4861 acgttcgtgt cctggtattc gtcgagcagc acggcgccga accggtcgcg gtactcctgg
     4921 cccacctcgg gatgtgtcgt ggcgatccgc atggcgagct cgatctggtc gccgaagtcg
     4981 atgaccccca ggtcgcgctt gagcctccgg aacacggccg aggccttcgc gagttcgatg
     5041 cgttccagcg ccgaatagta ggcgcggtcc gaccgattgc ctttcagtgc ctccagacgt
     5101 gaggtgcaga actccacgat ggcgtcgggc tcgaccaggt ggttctgggc ctgcgcgtcc
     5161 aggttgagga tcttgtcgac cacgctgggc tgccattcgg tcttgacgtg atcgaacgtc
     5221 atctcgtcga ggacgcggcc ggcgagctcc acccgctggg cctgactcag cacgcgctgc
     5281 cccggctcga tgccggcgag gatgccgtag cgatccagca cctgggccgc catggcgtga
     5341 tagttcacga tctcgggctc ttcgccctcg gggagatcca cgcccgccag cgcacggcgc
     5401 acccggctgc ggaggttctc c
//
LOCUS       DATDBZ010000177         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1061289, whole genome shotgun sequence.
ACCESSION   DATDBZ010000177 DATDBZ010000000
VERSION     DATDBZ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061289"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VIX62_04355"
     CDS             complement(<1..326)
                     /locus_tag="VIX62_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEY5859495.1"
                     /translation="MTSTKRIALVHLMQETNTFNPVPTTLADFEDLALLEGDEVLARS
                     DPSGPIAGAHAAVEASAADVELVPVLRADAQSGGRLTRDTLDELCRRLTHRLRESLPV
                     DGLLVLL"
     gene            complement(344..1138)
                     /locus_tag="VIX62_04360"
     CDS             complement(344..1138)
                     /locus_tag="VIX62_04360"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5859496.1"
                     /translation="MTVAPTAIGGRRLEGRVALITGASGGIGAAVAKAFAGQGASVVV
                     SSYPDPEMERLADEVVLSIVDAGGRAIRASADVTSPAQIEAAVAAARSELGDVDVLVA
                     NAAYSNRLPWHQISAEQWDHTHAVNLRGAFLCAQACREGMLRAGGGSIISVTSVTVEL
                     GMAGLLDYVSSKAGLIGFTRALAREVGADGIRVNAVMPGAIRTEQEVALGFDEEELKV
                     LSAERQCIPRRGFADDLSGAFVFLASDESSFVTGQVLNVDGGWVHY"
     gene            complement(1135..1740)
                     /locus_tag="VIX62_04365"
     CDS             complement(1135..1740)
                     /locus_tag="VIX62_04365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5859497.1"
                     /translation="MGTPHRYIVGDLTSDALYEELDQLPEHVDIVVNNAGGDPHSKPW
                     GQQTTQEWRDTYEINVVAAERLCRLFIPKMAARGWGRVINISSIYGSIGQNPDNTGRN
                     AGAGAYTAAKHGLIGLTNYLACQIGRTGVTINTLSPGMITWMETPDGEPELWKHLADQ
                     TPVGRNSQPEDYVTSVVFLASEGSSFVHGTNLVVDGGWSVW"
BASE COUNT          327 a          761 c          701 g          338 t
ORIGIN      
        1 agcaggacca acaacccgtc cacgggcaac gattcgcgca ggcggtgcgt gagtcgacgg
       61 cacagctcgt cgagggtgtc tcttgtcagc cgcccgcccg actgcgcgtc ggcgcgcagt
      121 acgggaacca gttcaacgtc agcggcgctc gcctcgaccg ccgcgtgcgc cccggcgatc
      181 ggcccgctcg gatcggacct cgcgagcacc tcgtcgcctt cgagcagcgc caggtcctcg
      241 aagtcggcga gcgtcgtggg cacggggttg aacgtgttcg tctcctgcat caggtggacg
      301 agcgcgatgc gcttggtgct ggtcaccgtc gacctccgag cgctcagtag tggacccatc
      361 cgccgtcgac gttcagcacc tgcccagtga cgaagctgct ctcgtcggac gcgaggaaga
      421 cgaatgcgcc cgacaggtcg tccgcgaacc cgcgtcgtgg gatgcactgg cgctcggccg
      481 acagcacctt cagctcttcc tcgtcgaagc ccaacgccac ctcttgctcg gtgcggatcg
      541 cgcccggcat gactgcgttc acccggattc cgtccgcgcc cacctcgcga gccagggctc
      601 gggtgaaccc gatgagcccg gccttggacg agacgtagtc caggagcccg gccatgccga
      661 gctcgacggt gacgctggtg accgagatga tgctgccgcc gcccgcgcgg agcatcccct
      721 cgcggcacgc ctgcgcgcac aggaaggcgc cgcgcaggtt caccgcatgc gtgtggtccc
      781 actgctcggc cgagatctgg tgccacggaa ggcggttgga gtaggcggcg ttcgcaacga
      841 gcacgtcaac gtcaccgagc tccgaccggg ccgctgctac cgcggcctcg atctgggcgg
      901 gtgacgtgac atcggcggag gcccggatgg cccggccgcc ggcgtcgacg atcgacagca
      961 ccacctcgtc ggccagccgc tccatctcgg ggtccgggta cgagctcacg acgacactcg
     1021 caccctgccc tgcgaacgcc ttggcgacgg ctgcgccgat gccccccgac gcgcccgtga
     1081 tgagcgcgac tcgaccctcg agccggcgcc caccgatcgc cgtcggcgca acggtcacca
     1141 cacgctccag ccgccgtcga cgacgaggtt cgtgccgtgg acgaacgacg acccctccga
     1201 cgcgaggaag acgaccgacg tcacgtagtc ctcgggctgg ctgttccggc ccaccggcgt
     1261 ctggtcggcc agatgcttcc agagctccgg ttcgccgtcg ggcgtctcca tccaggtgat
     1321 catccccggg ctcagggtgt tgatggtcac accggtgcga ccaatctggc acgccaggta
     1381 attggtcagg ccgatgaggc cgtgcttcgc cgcggtgtac gcgccggcac ccgcgttcct
     1441 gcccgtgttg tcggggttct gcccgatcga gccgtagatg gacgagatgt tgatgacgcg
     1501 gccccaccct cgggccgcca tcttggggat gaacagtcga cacagccgct cggccgcgac
     1561 gacgttgatc tcgtaggtgt cacgccactc ctgcgtcgtc tgctgtcccc acggcttcga
     1621 gtgcggatcg ccgcccgcgt tgttcacgac gatgtcgacg tgttcgggga gttggtcgag
     1681 ctcctcgtac agcgcatcgc tcgtgagatc gccgacgatg tagcgatgcg gcgtgcccaa
     1741 ggtggagaag gtttcgcgca gcgcccccct cgctgcgcgc cagcccgatc acggtcgcac
     1801 catgcgcggc gagcccatgg accaaccccc ggccgaggcc gtagccacca ccggtcacca
     1861 gtgcggtgcg cccggtgagg tcgaacagac cgttgcttgc tgccatcgtc caagccctcc
     1921 tgcttgtgca cccccagagg cggcgcgttc gacgcacgca gagcgtcagc gattgtttgc
     1981 gctgccgtct cccctgtgct acgaaatgct atagcacagg tggacacacg agctcgccca
     2041 aggggggaac atgaaggcac acgcgaggtc ccgcgcgcca ggtcgccgca gcatcgcggc
     2101 agcgttggcg gcggcgcttg cactcat
//
LOCUS       DATDBZ010000178         7015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2516692, whole genome shotgun sequence.
ACCESSION   DATDBZ010000178 DATDBZ010000000
VERSION     DATDBZ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516692"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2133)
                     /locus_tag="VIX62_04370"
     CDS             complement(<1..2133)
                     /locus_tag="VIX62_04370"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY5859498.1"
                     /translation="MAADTDEREVTLDIQGMTCASCVTTVERALGSVDGVTEASVNLA
                     TRTATVKGNAGEPDALFDAVRRAGYGAGPHVAIRDPGEEARSFRTRLIVAMVCTLPVL
                     WLTFVVPDPSWSRNAAWALTSVVVWFSGWPFFRSAAVAARHGRSTMDTLIAVGASAAY
                     SYSVYSVLSGGDALYFDTAAVIITLILVGKVLEATARASAGDAARVLLERGAKTATVL
                     DEGRETPTPIDRVVPGDLVLVRPGEKIPADGVVKTGASWVDLSLLTGESVPVEVGPGD
                     DVVGASINGNGRLVVFVTTVGANTALAGIVRSLERAQGSKAPVQQLADRVSAVFVPAV
                     LVLAGLTALGWVALAGATPGDAVLHAVAVLLIACPCALGLATPVAVMAGTGRAAELGV
                     LFKGAEVFERAKRVDIALLDKTGTITDGVMTLTDVAPAPPATADEVVAVAAAAEAGSE
                     HPIARAVLDGARDRGIDVRSATAHAAKPGAGMEADLDGTPIRVGRPAGLPPALSDEAE
                     RLASSGRTPFGVWRDDVPIGLIAVADRIKPETADAVVGLRASGLEVAMVTGDHRASAR
                     AIAREAGIEDVTAEVVPEGKVERIAALRTAGRHVAFVGDGLNDGPALAAADVGIAMGT
                     GTDVALAAADVNLLSGTLMAVVDAFALARRTYRIIGQNLFWAFAYNVVMIPLAVFGVL
                     EPMWAAAAMAASSVTVVLNALRLRRFGRV"
     gene            complement(2139..2414)
                     /locus_tag="VIX62_04375"
     CDS             complement(2139..2414)
                     /locus_tag="VIX62_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016456937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY5859499.1"
                     /translation="MYGYAEHKKDLLDRLRRVEGQVRGLQRMVDEDQYCIDILTQVNS
                     ATAALRAVGFGLLDDHVRHCVRESIEHGTGDEKVEELMAAVGRFAGK"
     gene            complement(2424..2591)
                     /locus_tag="VIX62_04380"
     CDS             complement(2424..2591)
                     /locus_tag="VIX62_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEY5859500.1"
                     /translation="MDTDPVCGMEVDREHAAAAWEHDGVTYRFCSVRCMERFRAEPER
                     YLRMDPSDRHM"
     gene            2803..3750
                     /locus_tag="VIX62_04385"
     CDS             2803..3750
                     /locus_tag="VIX62_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY5859501.1"
                     /translation="MYRTILTARLLDEAALRQNRMGRAPFVVPAEGHEACQVGTAWVM
                     RKGVDVWVPYYRDGAVVLAAGMTPFEIFLGIFAKADDPSSGGRQMPSHWGHKGLDIIS
                     GSSPIATQIPHAAGIAYAIKYRQEDAVVGCWFGDGATSEGDWHEGLNFAGIHRLPVIF
                     VCENNQYAISVPQAKQMAVDNVADRAEGYGFPGVVVDGNDVLACYGAMKQAHERARAG
                     EGPTLIECKTYRFLGHTSDDDDKTYRPRDEVEEARHNDPVHVFGEYLRTQEVLDDVGA
                     EELRLEIKGEIDTALAAAWDAADPTPESAMRHVFSEGEA"
     gene            3756..4745
                     /locus_tag="VIX62_04390"
     CDS             3756..4745
                     /locus_tag="VIX62_04390"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY5859502.1"
                     /translation="MSEKNVVTAIRDALHDEMAADDRVILLGEDVGNRGGVFRISAGW
                     MEEFGEDRVIDTPLAESGIVGVAIGMALRGLLPVAEIQFADFIHPAFDQIVSEAARLR
                     YRSNGAFGCPLVIRAPCGGGIHGALYHSQSIEAFYAHVPGIKVVMPSTPADAAGMLRS
                     ALRDPDPVLFLEHKKTYRAVKGEVPDGSYEIPIGPADVKREGTDLTCVSYGYSLHTCL
                     DAAARLQEDEGISIEVVDVRTISPLDTETIVASVRKTAKAIVVYEDNRSFGAGAEIVA
                     TIAEEAMFDLDAPIVRVGAPDIPAMPFATPLEHFFMEPEPDQIYRRMRDLAHH"
     gene            4896..>7015
                     /locus_tag="VIX62_04395"
     CDS             4896..>7015
                     /locus_tag="VIX62_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5859503.1"
                     /translation="MPRDLAVAGAVLAGASACVLTGASFGLVWGAVTAIGLSAAVLLA
                     MAATRRRDPAAAEDLDEGRFRAMVEQVPAIAYTWDTTRPTGEAPPVYISPQLETILGY
                     TPQEWSDHPELWLDAMHPDDRERVRRSSDDADRAGTTFHEEYRIFAKDGRMLWLRDDS
                     LVVARDEDGNAIRAQGVMFDITRQKQAEARLQEAENRYRTIVERVPAVAYVWDAANEP
                     GTAPAAYISPQIQRLLGYTPEEWFDDPNLWADRLHPEDVDGVFSRWDAAVAAAGAFTA
                     EYRIRSKAGELLWIRDEAIPVADGASGRPLYQGVMYDITEQRGTNERLREAEERYRTL
                     VENLPVVTYTAAVDEAGERDLLRYVAPGIERLTGYTPEAWTADAGFWESLIHPEDRDR
                     VVASSLACEDTGDPFDEEYRLIRADGAVIWVHDSAVVVEHDDEAAVWQGVFEDISARK
                     AAEQAVVQAEGRYRNLVEHLPAVVYIDEIDELATAHYVSPQYERLTGYSPAHRLATPD
                     LWVKMLHPEDRERVLAESDRTNESGEPFDIEYRIVRADGEVRWLHDHAFQVEALDGGM
                     AWQGVLTDVTDRKVAQDTLHRRDAILEAAGYAAERFLQAPAWRDGIDDVLARLGEQGG
                     ASRAVLFENLEIDGELQVQLRHAWLAAEAPAALDRPPSEAYPYGEDFRRWQQVLASGG
                     VIHGLVSELPERERPMIEASGIRSL"
BASE COUNT         1121 a         2530 c         2355 g         1009 t
ORIGIN      
        1 cacacgtccg aaccttcgaa ggcgcagggc attcagcacc accgtgacgc tggaggcggc
       61 catggcggcg gcggcccaca tgggctcgag caccccgaag accgcgagcg ggatcatcac
      121 cacgttgtag gcgaaggccc agaacaggtt ctgcccgatg atccggtagg tccggcgagc
      181 gagggcgaac gcgtcgacca ccgccatgag cgtgcccgag agcaggttga cgtccgctgc
      241 ggcgagcgcc acatcggtcc ccgtgcccat cgcgatcccc acgtcggccg cggcgagcgc
      301 cggaccgtcg ttgagtccgt cgccgacgaa cgccacatgg cgaccggcgg tgcgcagggc
      361 cgcgatccgt tcgaccttgc cctccgggac cacctcggcc gtcacgtcct cgatccccgc
      421 ctcgcgcgcg atcgctcggg cgctcgcccg gtgatctccc gtgaccatcg ccacctccag
      481 gcccgaggcg cgcagcccca cgaccgcgtc ggcggtttcg ggcttgatgc gatcggcgac
      541 ggcgatcagt ccgatgggaa cgtcgtcgcg ccagacaccg aacggtgtcc tacccgagga
      601 cgcgagtcgt tcggcctcgt ccgacagggc gggtggcagc cccgccggtc ttccaacccg
      661 gatcggggtt ccgtcgagat ctgcctccat cccggctcca ggcttcgcgg catgcgccgt
      721 ggcggaccgc acgtcgatcc ctcgatcgcg ggcgccgtcg aggaccgccc gcgcgatcgg
      781 atgttcggag ccggcttcgg cggcggccgc cacggccacc acctcgtcgg ccgtcgcggg
      841 aggcgccggc gcgacgtccg tcagcgtcat cacaccgtcg gtgatcgtcc cggtcttgtc
      901 gagcagggcg atgtccaccc gcttcgcgcg ttcgaagacc tcggcgcctt tgaacaggac
      961 ccccagctcg gcggcgcggc ccgtgcccgc catgacggcc accggggtcg cgagaccgag
     1021 cgcgcacgga caggcgatca gcagtaccgc cacggcatgg agcaccgcgt cgccgggtgt
     1081 cgccccggcg agcgcgaccc accccagggc tgtgaggccc gcgaggacga gcacggcggg
     1141 aacgaagacc gccgagacac ggtcggcgag ttgctgcacg ggcgccttcg agccctgcgc
     1201 ccgttccagc gatcgaacga tgcctgcgag cgcggtgttg gcccccaccg tggtgacgaa
     1261 caccaccagc ctgccgttgc cgttgatcga tgcacccacg acatcgtcgc cgggccccac
     1321 ctcgaccggc accgattctc ccgtcaacaa cgacagatcc acccacgaag cacccgtctt
     1381 cacgacccca tcggccggga tcttctcccc aggacggacc agcacgaggt cgcccggaac
     1441 cacacggtcg atcggggtgg gcgtctcgcg gccctcatcg aggacggtag cggtcttcgc
     1501 gcctcgttcg agcagcacgc gggcggcgtc gccggcgctg gcccgagccg tggcctcgag
     1561 caccttgccg accaggatca gcgtgatgat cacggccgcg gtgtcgaagt agagcgcgtc
     1621 gccgccggac aggaccgagt acacgctgta gctgtaggcc gcgctggccc ccaccgcgat
     1681 cagggtgtcc atcgtgctcc gcccatggcg cgcggcgacc gcggccgacc ggaagaacgg
     1741 ccacccggag aaccacacga ccacgctcgt cagcgcccag gcggcgttcc ggctccacga
     1801 gggatcgggc acgacgaagg tcaaccacag caccggcagg gtgcagacca tcgcgacgat
     1861 cagccgggtt cggaacgaac gagcttcctc gccgggatcc cggatcgcga cgtgcggccc
     1921 ggccccgtac cccgcccggc gcaccgcgtc gaacaacgcg tcgggctcgc ccgcgttccc
     1981 cttcaccgtg gcggtccggg tggccaggtt gacgctggcc tccgtgaccc catcgaccga
     2041 acccagggcg cgctcgaccg tggtgacgca cgaggcgcac gtcatgccct ggatgtccag
     2101 cgtgacctct cgctcgtcgg tgtcggccgc catgcccgtc acttcccggc gaaccggccc
     2161 accgcggcca tgagctcctc gaccttctca tcgccggttc cgtgttcgat gctctcgcgc
     2221 acgcaatggc ggacgtgatc gtcgagcagg ccgaatccca ccgctcgcaa ggcggccgtg
     2281 gcggagttca cctgggtgag gatgtcgatg cagtactggt cctcatcgac catccgttgg
     2341 aggcctcgga cctgcccttc gacgcgacgg aggcgatcga ggagatcctt cttgtgctcg
     2401 gcgtatccgt acatcgtgac tcctcacatg tggcggtcgg aaggatccat ccggaggtag
     2461 cgctcgggct cggcccggaa gcgttccatg cagcgcaccg agcagaaccg gtaggtgacg
     2521 ccgtcgtgct cccaggccgc cgccgcatgc tcgcgatcga cctccatgcc gcacaccggg
     2581 tcggtatcca tgcccacagt gtataccccc tgggggtatc tgacggccgt gacctcgctc
     2641 acccgaacgg acggcgatgc gggccattcg gctggtcctg acgtcctggg gccggtgtta
     2701 tgctcgccgc acggtggcag gagggattca caccacgtgt cgactcgggt agcgacgacc
     2761 ccgcacggcg ctctcggtct caccaccgag gacctgctgg cgatgtatcg caccatcctc
     2821 acggcacggc tcctcgacga agccgcgctc cgccagaacc gtatgggtcg cgcaccgttc
     2881 gtcgttccgg ccgagggcca tgaagcgtgc caggtgggca ccgcatgggt gatgcgcaag
     2941 ggcgtggatg tctgggtgcc gtactaccgc gacggcgcgg tggtcctggc cgccgggatg
     3001 accccgttcg agatcttcct cgggatcttc gccaaggccg acgatccctc ctcgggaggg
     3061 cgacagatgc cgtcgcactg gggacacaag ggcctggaca tcatcagcgg ctcctccccc
     3121 atcgccacgc agatccccca cgctgccggc atcgcctatg cgatcaagta ccggcaggaa
     3181 gacgccgtgg tggggtgctg gttcggcgac ggcgccacca gcgagggcga ctggcacgag
     3241 ggtctgaact tcgcggggat ccaccggttg ccggtgatct tcgtctgcga gaacaaccag
     3301 tacgcgatca gcgtcccgca ggccaagcag atggccgtgg acaacgtcgc cgaccgggcc
     3361 gagggctacg ggttccccgg cgtggtggtc gacgggaacg acgtgctcgc gtgctacggg
     3421 gccatgaagc aggcgcacga acgcgcccgc gccggcgagg gccccacgct gatcgagtgc
     3481 aagacctacc ggttcctcgg gcacacctcc gacgacgacg acaagaccta ccgtccgcgc
     3541 gatgaggtgg aggaggcccg ccacaacgac cccgtgcacg tgttcgggga gtacctgcga
     3601 acccaggagg tcctcgacga cgtcggggca gaggaactcc gtctcgagat caagggtgag
     3661 atcgacaccg cgctcgccgc cgcctgggac gccgccgacc ccacgcccga gtccgcgatg
     3721 cggcacgtgt tctccgaagg cgaggcctga gcccgttgag cgagaagaac gtcgtcaccg
     3781 cgatccgcga tgccctgcac gacgagatgg ccgccgatga ccgcgtcatc ctgctgggcg
     3841 aggacgtcgg caaccgcggc ggcgtcttcc gcatcagcgc cggctggatg gaggagttcg
     3901 gcgaggatcg cgtgatcgat acccccctcg ccgaatccgg catcgtcggc gtggcgatcg
     3961 gcatggccct gcgcggcctg ctcccggtgg ccgagatcca attcgccgac ttcatccatc
     4021 ccgcgttcga tcagatcgtg tcagaggcgg ctcgactccg ctaccgctcg aacggcgcgt
     4081 tcggatgtcc gctggtgatc cgcgctccct gcggcggcgg catccacggc gcgctctacc
     4141 actcgcaatc gatcgaggcg ttctacgcgc acgtccccgg catcaaggtg gtgatgcctt
     4201 ccacccccgc cgacgccgca gggatgctcc gcagcgcgct ccgcgatccc gaccccgtgc
     4261 tgttcctcga gcacaagaag acctaccgag cggtcaaggg cgaggtgccc gacgggtcct
     4321 acgagatccc gatcgggccc gccgacgtga agcgcgaggg caccgacctc acgtgcgtca
     4381 gctacgggta ctcgctccac acctgcctgg acgcggccgc ccgtctgcag gaggacgaag
     4441 gcatctcgat cgaggtcgtg gacgtccgga cgatctcacc cctggacacc gagacgatcg
     4501 tggcctccgt ccgcaagacc gcgaaggcca tcgtggtgta cgaagacaac cgctccttcg
     4561 gggccggcgc cgagatcgtc gcgaccatcg ccgaggaggc gatgttcgac ctcgacgccc
     4621 ccatcgtgcg ggtcggcgcg cccgacatcc cggccatgcc gttcgccaca cccctcgagc
     4681 acttcttcat ggaacccgag cccgaccaga tctaccgccg gatgcgcgac ctcgctcacc
     4741 actgacacga ggcggctcgt gccccgctga tcggctccgc tgcagagtgt tccgaaccca
     4801 cccctcgtct cggttcagga gggttcggaa cgcgtcgata cacggcggga gtggacgaac
     4861 atccgacgcc gacgccccga cggggacggc cctcggtgcc gcgcgacctc gccgtcgccg
     4921 gtgccgtgct ggccggtgcc tcggcgtgtg tgctgacggg tgcctcgttc ggtctcgtgt
     4981 ggggcgccgt cacggcgatc gggctctcgg cggccgtgct cctggcgatg gccgccacgc
     5041 gtcgtcgcga ccccgccgct gctgaggacc tcgacgaggg ccggttccgg gccatggtgg
     5101 agcaggtccc cgcgatcgcc tacacgtggg acaccacgcg tcccacgggc gaggcaccgc
     5161 ccgtctacat cagcccgcag ctcgagacga tcctgggcta caccccgcag gagtggagcg
     5221 atcaccccga gctgtggctg gacgcgatgc accccgacga ccgcgagcgg gtccggcgat
     5281 cgtccgacga cgccgatcga gccggtacga cgttccacga ggagtaccgg atcttcgcga
     5341 aggacgggcg catgctctgg ctccgcgacg actcgctggt ggtggcccgc gacgaggacg
     5401 gcaacgcgat ccgggcgcag ggcgtcatgt tcgatatcac ccgtcagaag caggccgaag
     5461 cccgtctgca ggaggcggag aaccgctacc gaacgatcgt cgagcgggtc ccggcggtcg
     5521 cctacgtctg ggatgccgcc aacgaaccgg gcacggcgcc ggccgcctac atcagcccgc
     5581 agatccaacg gttgctcggc tacacccccg aagagtggtt cgatgacccg aacctgtggg
     5641 ccgatcggct gcatcccgag gacgtcgacg gggtgttctc ccgctgggac gccgcggtgg
     5701 ccgccgcggg tgcgttcacg gcggaatacc ggatccgttc gaaggccggc gaactgctgt
     5761 ggatccgtga tgaggccatc cccgtcgcgg acggagcgtc gggccgcccc ctctaccagg
     5821 gcgtgatgta cgacatcacc gagcaacgcg gcacgaacga acgactccgg gaggcggagg
     5881 aacgctaccg gaccctggtg gagaacctcc ccgtcgtcac ctacaccgcc gcggtcgacg
     5941 aggccggaga gcgggacctg ctgcgctacg tcgcgcccgg gatcgagcgc ctgaccggct
     6001 acacgcccga ggcgtggacc gccgacgccg ggttctggga gtcgttgatc caccccgagg
     6061 atcgggaccg cgtcgtcgca tcgtccctgg cgtgcgagga caccggtgac cccttcgacg
     6121 aggagtaccg gctgatccga gccgacgggg ccgtcatctg ggtgcacgac tcggcggtcg
     6181 tggtcgagca cgatgacgag gccgccgtct ggcaaggcgt gttcgaggac atctccgctc
     6241 gcaaggccgc cgaacaggcc gtcgtccagg ccgaaggtcg ctatcggaac ctggtcgaac
     6301 acctgccggc ggtggtctac atcgacgaga tcgacgagct cgccacggcc cactacgtga
     6361 gccctcagta tgaacgcctc accggctaca gccccgcgca ccgcctggcc acgccggacc
     6421 tgtgggtgaa gatgctgcac cccgaggatc gggaacgcgt cctcgccgag tccgaccgga
     6481 cgaacgaatc gggtgagccg ttcgacatcg agtaccgcat cgttcgtgcc gacggcgagg
     6541 tccgctggtt gcacgaccac gccttccagg tggaagcgct cgacggcggc atggcctggc
     6601 agggcgtgct caccgacgtc accgatcgca aggtcgccca ggacaccctg caccgacggg
     6661 acgcgatctt ggaggcggcg ggctacgcgg ccgaacgatt cctgcaggcg cccgcgtggc
     6721 gcgacggcat cgacgacgtc ctcgcccggc tcggtgaaca gggcggggcc tcgcgcgccg
     6781 tcctgttcga gaacctggag atcgacggcg agctccaggt gcagctgcgg catgcatggc
     6841 tcgcggcgga agcgcccgcc gccctcgacc gcccaccgtc ggaggcctac ccctatggcg
     6901 aagacttccg tcggtggcag caggtcctgg cctccggcgg cgtgatccac ggcctcgtgt
     6961 ccgagctccc cgaacgagaa cgcccgatga tcgaagcatc gggcatcagg tccct
//
LOCUS       DATDBZ010000179         3616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_674353, whole genome shotgun sequence.
ACCESSION   DATDBZ010000179 DATDBZ010000000
VERSION     DATDBZ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674353"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VIX62_04400"
     CDS             <1..279
                     /locus_tag="VIX62_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859504.1"
                     /translation="LQIALVGVIVVAFGLVALARVSTRAFAATYVAGGLATWTVLFGW
                     RAGVSETDGANMFMAPLVILFLPATIVTPILVRAIAARLDGREGLVRA"
     gene            complement(323..1666)
                     /locus_tag="VIX62_04405"
     CDS             complement(323..1666)
                     /locus_tag="VIX62_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859505.1"
                     /translation="MTDHPPRHDAPVVFRRLLANTLVSGVTSSFLWFALTFWVYLETR
                     SVVATGVIGGSFGLASAAIGPAFGTYVDHHRKKAAMVLATTISASCFAVATAVFVAVD
                     SERLLEMRGPWFWILVASTLLGSVAGQMRGIALSTCVTLLVPEGDRDKANGLVGTVSG
                     LSFAITSVFSGLVIGQLGMGWALYGSLALTLGSLVHLRGIHIEEPEPEPAAAGAASRV
                     DIRGALDAIHAVPGLSMLIALAAFNNLLTGVFMALLDAYGLSLVSVETWGFLFGGISM
                     AFIVGGLLVARFGLGKLPLRTILLGNLVNWTVCSVFTLHSSIVPLVIGMFVWLLLVPV
                     IEAAEQTVLQRSIPFERQGRVFGFAQLVENAAAPVTAFLIGPLAESVVMPLMTNGRGA
                     DAIGEWFGTGPERGLALMFTLAGLIGVIITLAARASRSYRELSAGWLALDAAAAA"
     gene            1760..2671
                     /locus_tag="VIX62_04410"
     CDS             1760..2671
                     /locus_tag="VIX62_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5859506.1"
                     /translation="MSPERLDRPAPKVALVTGGAGFVGSHLIRRIVEQFPETSVISID
                     NYFTGSVDNHVNDPRVQYITASCLDINRVWDRRRLPSPDVTFHLGEYSRIVQSFGDFD
                     DVWEFNIRGTKEVVAFCHERGSLLVYAGSSSKFASEGAEHLSPYSWTKAKNIEFIRNF
                     STWFGLEYVITYFYNVYGPGQIGKGAYATVIGIFEEQAANGVALTVVEPGTQTRDFTH
                     VDDIVDGLIVCYLHGEGDGYELGTSQDWEIIEVARLFSDDIELVPARQGERTRGKADT
                     TKARSIGFEPKRSLADYAERIRKEREG"
     gene            complement(2672..>3616)
                     /locus_tag="VIX62_04415"
     CDS             complement(2672..>3616)
                     /locus_tag="VIX62_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5859507.1"
                     /translation="GIAPPISSDPPFHHGARRVLLPAFAPQAIDKLEASTRDYCKELI
                     AEMGDREVVDAAKEYAQHIPVRVIARMLGFPEEDADRFRGFVHHVLEGVALPFEEREE
                     GMIVLFEYLEAQVQDHVENPRDDLTTFLLESKLDGEPLNMDHVGGTMALLLLAGIDTT
                     WSAIGASIFHLASTPADLARLVAEPELLPTAMEELLRAYAPVTMARLVRDDTDFHGCP
                     MKAEDWVLLPFPAANRDPLAFEDADQVIIDREVNRHAAFGLGIHRCAGSHLARMELRV
                     ALEVWLEHFPRFSLADPDAVQWSGGQVRGPRVLPIRIG"
BASE COUNT          609 a         1187 c         1303 g          517 t
ORIGIN      
        1 ctgcagatcg cgctcgtcgg tgtgatcgtg gtcgcgttcg gccttgttgc actcgcccgc
       61 gtctccacac gtgcgttcgc agcgacgtac gtcgcaggag ggctggcgac gtggacggtc
      121 ctgttcgggt ggcgggcagg agtctcggag accgacgggg cgaacatgtt catggccccg
      181 cttgtgatcc tgttccttcc agcgacgatc gtgaccccga tcttggttcg cgcgatcgct
      241 gcccggctgg atggccggga gggcctcgtc cgggcatgac tgccgatgtg ggtcaccact
      301 gcgttggccg tcgtcggacg cttcaggcgg ccgcggctgc gtcgagggcc agccagcctg
      361 cgctcagctc gcggtaggag cgtgatgccc gtgccgcgag ggtgatgatc acgccgatca
      421 ggccggcgag cgtgaacatg agggcgaggc cgcgctcggg accggtgccg aaccactcgc
      481 cgatggcgtc ggcaccgcgg ccattcgtca tgaggggcat cacgaccgac tccgcgagcg
      541 ggccgatcag gaacgccgtg acgggggcgg cggcgttctc gaccagctgg gcgaagccga
      601 acacgcgacc ctgccgctcg aacgggatcg agcgctgcag cacggtctgc tcggccgcct
      661 cgatcaccgg gaccaagagg agccacacga acatcccgat gacgagcggg acgatcgagg
      721 agtgcaacgt gaagacggag cacaccgtcc agttgacgag gttgccgagc aggatcgtgc
      781 ggagcggcag cttgcccagc ccgaaccgcg cgaccaggag gccgccgacg atgaaggcca
      841 tgctgatgcc accgaagagg aacccccacg tctcgacgga cacgagcgac aacccgtagg
      901 catcgagcaa cgccatgaag acgccggtga gcaggttgtt gaacgccgcc agcgcgatca
      961 gcatcgagag cccgggcacc gcgtggatgg cgtcgagcgc gccgcggatg tcgactcggg
     1021 aggcagcgcc ggcggcggcg ggttccggct cgggctcctc gatgtggatc ccgcggaggt
     1081 ggaccagcga gcccagggtg agggccagcg agccgtacag cgcccagccc atgccgagct
     1141 gaccgatcac cagcccgctg aacacggacg tgatggcgaa cgagagcccg ctgaccgtcc
     1201 cgaccaggcc gttggccttg tcgcggtcgc cctccggcac cagcagcgtc acgcaggtcg
     1261 acaaggcgat gccccgcatc tgcccggcga ccgatcccag gagggtgctg gccaccagga
     1321 tccagaacca gggaccgcgc atctcgagca ggcgctcgct gtcgacagcc acgaagacgg
     1381 cggtggccac cgcgaagcac gacgcggaga tggtcgtcgc cagcaccatc gccgccttct
     1441 tgcggtggtg atcgacgtag gtgccgaacg cggggccgat ggcggcggac gccaggccga
     1501 aggacccgcc gatcacgccg gtggccacca cggatcgggt ctcgaggtac acccagaagg
     1561 tcaaggcgaa ccagaggaag gagctcgtca ccccgctcac cagcgtgttg gcgaggaggc
     1621 gtcggaagac gaccggcgcg tcgtgccgcg gggggtgatc agtcacgagc gcgccggtca
     1681 tgcagatgag gacgactcgc cgcgctcgaa gtcatcggac ggctcggggc cgcatccggg
     1741 cccggttagc ctccgggccg tgagtcccga gcgcctcgac cgtcccgcgc ccaaggtcgc
     1801 actcgtcacc ggcggggccg gcttcgtggg cagccacctg atccggcgca tcgtggagca
     1861 gttccccgag acgtcggtga tctcgatcga caactacttc acgggctcgg tggacaacca
     1921 cgtcaacgac ccgcgggtgc agtacatcac cgcgagctgt ctggacatca accgggtgtg
     1981 ggatcgccgg cgcctgccgt cgcccgacgt cacgttccac ctcggtgagt actcccggat
     2041 cgtgcagtcc ttcggcgact tcgacgacgt gtgggagttc aacatccgcg gcaccaagga
     2101 ggtggtcgcg ttctgccacg agcgcgggtc gcttctcgtc tacgcggggt ccagctccaa
     2161 gttcgcgagc gagggggcgg agcacctcag cccgtactcc tggaccaagg ccaagaacat
     2221 cgagttcatc cgcaacttct cgacgtggtt cgggctcgag tacgtgatca cctacttcta
     2281 caacgtctac gggcccggac agatcggcaa gggcgcctac gccaccgtca tcgggatctt
     2341 cgaggagcag gcggcgaacg gcgtcgcgct gaccgtcgtc gagcccggca cgcagacccg
     2401 tgacttcacc cacgtcgacg acatcgtgga cgggctcatc gtgtgctacc tccacgggga
     2461 gggagacggg tacgagctcg gcacgtccca ggactgggag atcatcgagg tcgcccgctt
     2521 gttcagcgac gacatcgagc tggtgccggc ccggcagggt gagcgcaccc gcggcaaggc
     2581 ggacaccacg aaggcacgct cgatcgggtt cgagccgaag cggtctctgg ccgactacgc
     2641 cgagcggatc cgcaaggagc gagagggctg atcaaccgat ccggatcggc aggacgcggg
     2701 ggccgcgcac ctggccgccg gaccactgga cggcgtccgg gtcggcgagg ctgaagcgag
     2761 ggaagtgctc gagccacacc tcgagcgcca cgcgcagctc catgcgggcg aggtgcgaac
     2821 cggcgcaccg gtggatgccg agcccgaagg cggcgtggcg gttcacctcg cggtcgatga
     2881 tcacctggtc ggcgtcctcg aacgcgagcg ggtcgcggtt ggccgcgggg aacgggagga
     2941 gcacccagtc ctcggccttc atcgggcagc cgtggaagtc ggtgtcgtct cgaaccaggc
     3001 gggccatggt cacgggggcg taggcacgga ggagctcctc catggcggtc gggagcagct
     3061 ctggttcggc gaccagccga gccaggtcgg cgggggtgga cgcgaggtgg aagatcgatg
     3121 ccccgatggc gctccacgtc gtgtcgatgc cggccaggag cagcagggcc atcgtgcccc
     3181 cgacgtggtc catgttgagc ggctcgccgt cgagcttcga ctccaggagg aaggtcgtga
     3241 ggtcgtcgcg tgggttctcg acgtggtctt gcacctgggc ctcgaggtac tcgaagagga
     3301 cgatcatccc ctcctcgcgc tcctcgaacg gcagcgcgac tccttcgagg acgtggtgga
     3361 cgaagccgcg gaagcggtcg gcgtcctcct ccgggaaccc cagcatccga gcgatcacgc
     3421 gcaccgggat gtgctgggcg tactccttcg cggcgtcgac cacctcgcga tcgcccatct
     3481 cggcgatgag ctccttgcag tagtcgcgcg tggaggcctc gagcttgtcg atcgcctggg
     3541 gggcgaacgc cggcaggagc actcgccgcg cgccgtggtg gaacggcggg tcagaggaga
     3601 tgggcggggc gatccc
//
LOCUS       DATDBZ010000180         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_36682,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000180 DATDBZ010000000
VERSION     DATDBZ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36682"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VIX62_04420"
     CDS             complement(<1..741)
                     /locus_tag="VIX62_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY5859508.1"
                     /translation="MTLARPETDPNVTPRRLLLLAVPAILVGVVCALILWTLDRAADA
                     LSNVIWDTLPGALNVDPDGWWIILVLTITGLAIGIALQVLPGHGGPDSATTELIGKPL
                     PLRTLPGLALVTLLGLAGGVSLGPENPIIAINCAIMVAIFARLIPQVSSQIAMLLAAA
                     ATVGALFGTPVAAALVFTGLVAAVKAGGSLWDKLFLPLAAAGAASITMHLLGAPPFAF
                     DMPAYGPPQALDILTGVVIACAVTVVGLA"
     gene            complement(843..1232)
                     /locus_tag="VIX62_04425"
     CDS             complement(843..1232)
                     /locus_tag="VIX62_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEY5859509.1"
                     /translation="MSISALTDDGRRFVSDYHLATLSTMGPSGGIHVVAVGFTFVDGV
                     VRIITSDASQKVRNIERDARATVAQLSGPQWLSIAGSGVIQRDADAVEHAVRLYAGRY
                     RQPRVNPRRVVITITPEKVLGSAGLWD"
     gene            1382..1771
                     /locus_tag="VIX62_04430"
     CDS             1382..1771
                     /locus_tag="VIX62_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018186871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY5859510.1"
                     /translation="MKITSTSDWREEIPFETPMIVADLVPGDPTRCSVCGGDSELRPR
                     TELWAVKHRHPNQHAGYVRFYCQPHLPVVERVVPVETRRTIARAERTAAMRRTPSVDE
                     KPRAMCPDCFVEISATGVCGMCGRQVA"
     gene            1804..>2107
                     /locus_tag="VIX62_04435"
     CDS             1804..>2107
                     /locus_tag="VIX62_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5859511.1"
                     /translation="MIFDHEAPEKYTPHADPAVIDDLRERVRATRWPDAPEDAGWTLG
                     TDLTYLRELAEYWVDGFDWRASEEQIARLPHFRVELGGIGIHVVHVRATDRIRPVVP"
BASE COUNT          353 a          702 c          738 g          314 t
ORIGIN      
        1 agcaagcccc acgaccgtca ccgcgcacgc gatcacgacg ccggtcagga tgtcgagcgc
       61 ctgagggggt ccgtacgcgg gcatgtcgaa tgcgaacggg ggggcgccca gaaggtgcat
      121 cgtgatggat gccgctcccg cggccgcgag gggcaggaac agcttgtccc agagcgagcc
      181 ccccgctttg accgcagcca ccaggcctgt gaacacgagc gccgccgcca ccggcgtgcc
      241 gaacagggcc ccgaccgtcg ctgccgccgc cagcagcatc gcgatctgtg aggacacctg
      301 cgggatcagc cgggcgaaga tcgcaaccat gatcgcgcag ttgatcgcga tgatcggatt
      361 ctcgggtccg agactcactc cgcccgcgag accgagcaga gtgacgagcg cgaggccggg
      421 cagcgtgcgc agcggcaaag gtttgccgat gagctcggtc gtggccgagt caggcccgcc
      481 gtgcccaggt agaacctgca gcgcgatgcc gatcgccagc ccggtgatcg tcagcacgag
      541 gatgatccac cagccgtccg gatcgacgtt gagggcgccg ggaagcgtgt cccagatcac
      601 gttcgaaagg gcgtcggccg cacggtcgag cgtccacagg atcagcgcgc agaccacccc
      661 taccaggatc gccggcacgg cgagcagcag aagccggcga ggtgtgacgt tcgggtcggt
      721 ctccggccgt gcgagggtca tgggcggatt ttcctcctgc tcgaccatgg cgagtcatgg
      781 tgatggacgg aacgtatcac gcaccacgtt cggcgtgcga tggccggcgc cggtgggaaa
      841 cgtcagtccc agaggccggc cgagccgaga accttctcgg gcgtgatcgt gatgaccacg
      901 cggcgagggt tcacccgcgg ttggcgatag cggccggcgt agagccgcac ggcgtgctcc
      961 accgcgtcgg catcccgctg gatcactccg ctgccggcga tactgagcca ctgcgggcca
     1021 gacagctgcg cgacggtcgc gcgcgcatcc cgttcgatgt tgcgcacctt ctggcttgcg
     1081 tcgctcgtga tgatccgcac gacgccgtcg acgaaggtga agccgacggc caccacgtgg
     1141 atgccgcccg acgggcccat cgtggagagt gtcgcgaggt ggtagtcgga cacgaaccgg
     1201 cggccgtcgt cggtcagggc ggaaatgctc atgctcccat catgggtgcg atgcgcgggg
     1261 gcggatccgc gaggggaccc ggactacgct ggggcaaggg tcatcgctca tcgctgaaca
     1321 gcgatcgcgt ccgccgccgc tcgccgcgac gtcggcttga tcccgctttt ggaggacacg
     1381 catgaagatc acctcgacat ccgattggcg cgaggagatc ccgttcgaga ctccgatgat
     1441 cgtggctgat ctggtccccg gtgatccgac gagatgctcc gtctgcggcg gggactccga
     1501 gctgcgtccc cggaccgagc tctgggcggt gaagcaccgg catccgaatc agcatgccgg
     1561 ctacgtgcgc ttctactgcc agccgcatct tccggtggtc gagcgggtcg tcccggtcga
     1621 gacgcggcgc acgatcgccc gcgccgagcg cacagccgcg atgcggcgca ctccttcggt
     1681 cgacgagaag ccgagggcga tgtgccccga ctgcttcgtc gagatctcgg cgaccggtgt
     1741 gtgcggcatg tgcgggcgac aggtggcctg accttcgtcg caccccgccg gtagcctgcc
     1801 cacatgatct tcgatcacga ggcaccggaa aagtacacac ctcacgcgga tcccgcggtg
     1861 atcgacgacc tccgcgaacg ggtgcgcgcc acgagatggc ccgacgcgcc cgaagacgcg
     1921 gggtggacgc tcggcacaga cctgacctac ctgcgtgaac tggcggagta ctgggtcgat
     1981 ggcttcgact ggcgcgcgtc ggaggagcag atcgcgcggc tgccgcattt ccgggtcgag
     2041 ctcggcggca tcggcatcca tgtggtccac gtacgagcca ccgaccgcat ccgccctgtg
     2101 gtgccgc
//
LOCUS       DATDBZ010000181         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_674517, whole genome shotgun sequence.
ACCESSION   DATDBZ010000181 DATDBZ010000000
VERSION     DATDBZ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674517"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1205)
                     /gene="moaA"
                     /locus_tag="VIX62_04440"
     CDS             complement(177..1205)
                     /gene="moaA"
                     /locus_tag="VIX62_04440"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY5859512.1"
                     /translation="MSEPSTSPPSSRPTSGPLLDTFGRAGSDLRISVTDRCNFRCTYC
                     MPAEGLAWLPKNEILTFEELTRLLALFVRLGVNSIKVTGGEPTVRADLPTLVRMFRGV
                     GPDLDISITTNGVLLDTLAAPLAEAGVDRATVSCDSLLTHRFADMTRRDALEKVLRGL
                     KAAEAAGLTPIKINCVVIGGTNDDEVVEFARWSRETGYQVRFIEYMPLDAQHAWEREK
                     VVPAATILERIGAVYPLRSTGPDGEPATTFTFADGEPGSIGVIPSVTEPFCDSCNRMR
                     VTAEGQFRSCLFALEETDLRTPLREGASDDDLEQLIRDAMWAKWSGHRINHPDFVQPA
                     RSMSMIGG"
     gene            complement(1242..1499)
                     /locus_tag="VIX62_04445"
     CDS             complement(1242..1499)
                     /locus_tag="VIX62_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HEY5859513.1"
                     /translation="MSTWTDRLAEALSVTPLEPEDEARLLDAAREVAHRVERRDTPLS
                     AFLMGVAVGTLTAQGTEREEAIDDAFEDLARILPDPPSTPA"
     gene            1568..>2104
                     /locus_tag="VIX62_04450"
     CDS             1568..>2104
                     /locus_tag="VIX62_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY5859514.1"
                     /translation="METEALAAVITVSDGVSQGTRTDGSGEQARSLLQAAGYRVAMST
                     VVPDEQPEISAAIREAAEYHALVVTTGGTGFGPRDVTPEATRAVIEREAPGLAELMRA
                     AGLAHTPMAALSRGVAGTVGRAVVVNLPGSPKGVKEGLEAVLPVLPHAVELSAGVTGP
                     HPTGHTGSPSGGPDIEKPI"
BASE COUNT          332 a          695 c          748 g          329 t
ORIGIN      
        1 tcccgcctga tcggcgagga ctccacggac cgaagggggt ccgcgggagg ccgggccgtg
       61 cccggcctcc cgcgccgagg agggggcgcg tcctcgttcg gcatgggtca tgctgtcagc
      121 ggcaggtgaa cggtccgtga cctgggggga acccgcaggc gaacgtgtac cggcgttcag
      181 ccgccgatca tggacatgct gcgggcgggt tggacgaagt ccgggtggtt gatgcggtgg
      241 ccggaccact tggcccacat cgcgtcacgg atcagctgtt ccaggtcgtc atccgaggcg
      301 ccctcacgca gcggggtgcg caggtcggtc tcctccaggg cgaacaggca gctgcggaac
      361 tgtccctcgg cggtgacgcg catgcgattg cacgaatcgc agaacggttc ggtgacgctg
      421 gggatgaccc cgatcgaacc gggctcacca tcggcgaacg tgaacgtcgt cgcgggttcg
      481 ccgtcggggc cggtcgagcg caagggatac accgctccga tccgttccag gatggtggcg
      541 gccggcacga ccttctcgcg ttcccacgcg tgttgggcgt ccagcggcat gtactcgatg
      601 aagcgcacct ggtagccggt ttcgcgggac cagcgggcga actccacgac ctcgtcgtcg
      661 ttcgtgccac cgatcacgac gcagttgatc ttgatcggcg tgagcccggc cgcctcggca
      721 gccttcaggc cccgcagcac cttctcgagc gcatcacgtc gtgtcatgtc ggcgaaccga
      781 tgcgtcagca gtgagtcgca cgacaccgtg gctcggtcca cgcccgcctc ggcgagcggc
      841 gccgccaagg tgtccaacaa cacgccgttg gtcgtgatgg agatatctag gtcagggccg
      901 acgccgcgga acatgcgcac cagggtcggc aggtctgccc gcacggtggg ctccccgccg
      961 gtgaccttga tcgagttcac gccgaggcgg acgaacaacg ccaggagcct ggtcaactcc
     1021 tcgaacgtca ggatctcgtt cttggggagc caggcgagac cttcggccgg catgcagtag
     1081 gtgcaccgga agttgcatcg gtcggtgacc gagatgcgca gatcgctccc cgcacgaccg
     1141 aacgtgtcga gcagcggtcc cgaggtaggt ctggaggatg gtggggaggt ggagggttcg
     1201 ctcatctgac cgccagccta ccgccggtcg acacgaacgc gctacgccgg ggtcgacggt
     1261 gggtccggca ggatccgagc cagatcttcg aaggcatcgt cgatggcctc ttctcgctcc
     1321 gttccctgcg cggtgagcgt gccgaccgcc acccccatga ggaaggccga tagcggagtg
     1381 tctctgcgtt cgacgcggtg cgcgacctcg cgagcggcat ccagcaaccg ggcctcgtcc
     1441 tcgggttcca gcggggtgac cgacagggct tcggccaacc gatccgtcca agtgctcatc
     1501 gcgggacctc cttcgtgcct cggtgctgac cgtaccgcgc gtgcgtccga tgcgagagga
     1561 tgggtacatg gagacggaag cgctcgcggc ggtcatcacc gtgtcggacg gcgtgtcgca
     1621 gggaacaagg accgacggat cgggcgaaca ggcacgatcc ctcctccagg cagccggcta
     1681 tcgggtcgcg atgagcaccg tcgtgcccga tgagcagccc gagatctcgg cggcgatccg
     1741 cgaagccgcc gagtaccacg ccctggtggt gacgacgggc ggcaccgggt tcgggccgcg
     1801 cgacgtcacg ccggaggcca cgcgagccgt gatcgagcgt gaagcgccgg gcctcgccga
     1861 actgatgcgt gccgccggcc tcgcccacac accgatggca gcgttgtcac ggggggtcgc
     1921 gggcaccgtg ggccgtgccg tcgtcgtcaa ccttcccggt tccccgaaag gtgtgaagga
     1981 gggcctcgag gcggtgctcc cggtcctgcc gcatgcggtg gaactgtctg cgggggtcac
     2041 gggtccgcat ccaaccggcc acacgggatc cccctcgggt ggcccggaca tcgagaagcc
     2101 catc
//
LOCUS       DATDBZ010000182         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1062987, whole genome shotgun sequence.
ACCESSION   DATDBZ010000182 DATDBZ010000000
VERSION     DATDBZ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062987"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VIX62_04455"
     CDS             <1..495
                     /locus_tag="VIX62_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5859515.1"
                     /translation="APKKDDDARSRPVGAGMVSGGTIIDGLIREAKVTGKWNDPVIRD
                     RIVDLYITGRVSALNIQRAAAKRKAGQQPGPEGSIAKLFGTEFNMAAQILSIDLMGGA
                     MAWEEGDGASAMRARGFLRSRGNSIEGGTSEIQRNIIGDRVLGLPRDPDPYKGQPWKD
                     VPRN"
     gene            550..957
                     /locus_tag="VIX62_04460"
     CDS             550..957
                     /locus_tag="VIX62_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5859516.1"
                     /translation="MADTSAPDPQADRALAQVFVTDFDRSLAFYRDVLGFDVVFTYGD
                     PAFYGEVSRGDAAFNLRHVDDSPFVAGVRDAGQLLSVSMRITDAALLFVEYEAAGVEF
                     QERLRDKPWRDSEFVVRDPDGNLILFGSPTEQR"
     gene            complement(1080..2357)
                     /locus_tag="VIX62_04465"
     CDS             complement(1080..2357)
                     /locus_tag="VIX62_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5859517.1"
                     /translation="MTIDTAPHATLPNPMIAEFWTRPAPEIDTDIAELLAAGTSFHDF
                     GFDDELVANSPLPDQGDGYWAVTRHSDILHVSKNAKIFSSAKGVTALDLPPEFNEFFS
                     SMIGMDDPRHARLRRIVSAGFTPRMLKLLEDSVQRIAGEIVDEIAERGECDFVVDVAA
                     RLPLRIVCDLMGIPESQHDFVFERTNMILGASDPEYMPQDGDILTGLLTAGGDLAALM
                     EEVAESKKGGDGQDLTSILVNAELPDDQLSHGDLASFFNLLVVAGNETTRNAISWGLL
                     YLTQNPEQRAIWADDFDGVAPTAVEEIVRLASPVTYMRRNALEDTHIGDTEIAAGDKI
                     CMFYLAANRDEAVFEDPLRFDVRRSPNPHVGFGGPGPHFCLGAHLARREITVMFRELF
                     DRLPDIEASGEPQLLRASFIHGVKHLPATFSPA"
BASE COUNT          391 a          763 c          873 g          406 t
ORIGIN      
        1 gccccgaaga aggacgacga tgcccgcagc cgcccggtcg gcgccggcat ggtcagcggc
       61 ggcacgatca tcgacggact catccgcgag gccaaggtca ccggcaagtg gaacgacccg
      121 gtcatccgtg accgcatcgt cgacctctac atcaccggtc gagtctcggc actcaacatc
      181 cagcgagccg cggccaagcg caaggccgga cagcagcccg ggcccgaggg ctcgatcgcc
      241 aagctgttcg gcaccgagtt caacatggcg gcccagatcc tgtcgatcga tctcatgggt
      301 ggcgccatgg cgtgggaaga aggcgacggg gccagcgcca tgcgggcccg cggcttcctg
      361 cgctcgcgcg gcaactccat cgagggcggc acgtcggaga tccagcgcaa catcatcggc
      421 gaccgcgtcc tcggtctgcc tcgggatccc gacccctaca agggtcagcc gtggaaggat
      481 gtcccccgca actgagggac cacagagccg gtgtcggccc tcgtgatgcc gatccgagga
      541 ggcttgcgca tggccgacac gtccgctccc gatccgcagg cggatcgtgc gctcgcccag
      601 gtgttcgtga cggacttcga ccggtccctg gcgttctacc gggacgtgct cggcttcgac
      661 gtcgtgttca cctacgggga tccagccttc tacggcgagg tctcacgagg cgacgcggcg
      721 ttcaacctgc gtcacgtcga tgactcgccg ttcgtggcgg gggtgcgcga cgccgggcag
      781 ctgctctccg tctcgatgag gatcaccgat gcagcgttgc tgttcgtgga gtacgaggcg
      841 gcgggcgtcg agttccagga acggctgaga gacaagccgt ggcgtgactc ggagttcgtg
      901 gttcgcgatc ccgacggcaa cctgatcctc ttcggttcac cgaccgagca gcggtagcgc
      961 tagagcacgg cgtctcggac tccgtgagcg atctcgcgcg cctgacccgc gcgatcgctc
     1021 acagagacgg cgggggcgtt ctcgctggcc ccgcctcccg gctcggagtt gatgccgcgt
     1081 caggcggggc tgaaggtggc cggcaggtgc ttcacgccgt ggatgaagct ggcgcgcagc
     1141 agttgcggtt cgccagaggc ctcgatgtcg ggcaggcggt cgaacagctc gcggaacatc
     1201 acggtgatct cgcggcgagc gaggtgtgca ccgaggcaga agtgcggacc aggcccgccg
     1261 aagcccacgt gggggttggg gctgcggcgc acgtcgaacc gcaggggatc ctcgaacaca
     1321 gcctcgtcgc ggttggctgc caggtagaac atgcagatct tgtcaccggc ggcgatctcg
     1381 gtgtcaccga tgtgggtgtc ctcgagggcg ttgcgtcgca tgtaggtgac ggggctggcc
     1441 aggcggacga tctcctcgac tgcggtcggg gccacgccat cgaagtcgtc ggcccagatc
     1501 gcccgttgct cggggttctg ggtgaggtag agcaggcccc aggagatggc gttgcgcgtc
     1561 gtctcgttgc cggcgacgac cagcaggttg aagaagctcg cgaggtcgcc gtgggagagc
     1621 tggtcgtcgg ggagctcggc gttgaccagg atcgacgtga ggtcctggcc gtcgccaccc
     1681 ttcttggact cggcgacctc ctccatcagc gctgcgaggt cgccgcccgc ggtgagcaga
     1741 ccggtgagga tgtcgccatc ctggggcatg tactcgggat cggatgcgcc gaggatcatg
     1801 ttggtgcgct cgaacacgaa gtcgtgctgg gactcgggga tgcccatcag gtcgcacacg
     1861 atccgaaggg gcagtcgggc ggcgacgtcg acgacgaagt cgcactcgcc gcgctcggcg
     1921 atctcatcga cgatctcacc ggcgattcgc tgcaccgagt cctcgaggag cttgagcatc
     1981 cgcggggtga agcctgctga aacgatgcga cgcaggcggg cgtggcgtgg gtcgtccatg
     2041 ccgatcatcg agctgaagaa ctcgttgaac tcgggtggca ggtcgagcgc ggtgaccccc
     2101 ttggccgagc tgaagatctt ggcgttcttc gacacgtgga ggatgtcgga gtgacgggtc
     2161 accgcccagt agccgtcgcc ttgatccggc agcggcgagt tggccacgag ctcgtcgtcg
     2221 aatccgaagt cgtggaagct ggtgccggct gccaacagct cggcgatgtc ggtgtcgatc
     2281 tcgggtgcgg gtctggtcca gaactccgcg atcatcgggt tcggcaaggt ggcgtggggc
     2341 gctgtgtcga tggtcacaga cgcgaccgta gctcgcgtct caggaggccg cccgccgtgc
     2401 ctggcgcgac tgacgccatg ccaacaggac gat
//
LOCUS       DATDBZ010000183         2974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2923396, whole genome shotgun sequence.
ACCESSION   DATDBZ010000183 DATDBZ010000000
VERSION     DATDBZ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2923396"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VIX62_04470"
     CDS             complement(<1..762)
                     /locus_tag="VIX62_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5859518.1"
                     /translation="MVKRFGEVVAVDGLSFDVGRREFFSLLGPSGCGKTTTLRMLAGF
                     ETPTEGRILVDGRDASRLAPHERDTNMVFQSYALFPHMTVAQNVAFGPQRKKLARKEV
                     EQRVQESLSAVRLEGYGKRYPRELSGGQAQRVALARALANQPSVLLLDEPLGALDLKL
                     REEMQFELKRIQREVGISFVYVTHDQGEALTMSDRIAVMNGGVVEHLGTPEEVYLSPA
                     SLFVAGFIGQTSFLSGRVESVGADGAEVSIEGGHRS"
     gene            complement(860..1771)
                     /locus_tag="VIX62_04475"
     CDS             complement(860..1771)
                     /locus_tag="VIX62_04475"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859519.1"
                     /translation="MVATAPPVSPSTEDTTRARPKGRWAGPIYSIAPTMVLLALFLGP
                     MFILLYYSFQKASLAGAGGWTFDNYTSILTDPTYGKVMLTTVMICTISMLIMLAIGLP
                     LAYILAMRTPPRFELLLLLMLVIAEELNPLIRIYAWRMILGRGGVINWTLQTIGLIDK
                     PIDSLLFSSTAVVIVLSTTYISFTTIPIYAALKAVDPDLLEAADDLGASFFTRCRKIL
                     LPLAAPGIFVAIILVYIPLYSEFAAPALVGGTSGYMIGNAIQEAILESGNRGVGSAMS
                     FMLLVLSGAVAWIGYRMAKIRRLQATS"
     gene            complement(1765..2673)
                     /locus_tag="VIX62_04480"
     CDS             complement(1765..2673)
                     /locus_tag="VIX62_04480"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859520.1"
                     /translation="MSATTTKIEPEATAGDQRGRGFSVGGREGRARAAAITRWVFLAI
                     VVVILYGPLLILALFSFNDSIVIALPFKGFTLEWYREALQNPLVRESLKNSLAVALVV
                     TPVALILGTMAAFATTRFRYKGRGSVAALGAGPLIVPWLILAVGALLFFSKLKVPLNL
                     RTIAVMHIVVAFPLVAAIVTARLIRFDHRMEEAARDLGATNREVLRYITLPHLVPALA
                     AAAILAFSYSFNNFVISFFVGGFELTFPIWVFSTLRHAQNVPIVNAISTLVSLAQLLI
                     VYVAWRLWRRRSKKMAANDDPMDMLW"
     gene            complement(2732..>2974)
                     /locus_tag="VIX62_04485"
     CDS             complement(2732..>2974)
                     /locus_tag="VIX62_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859521.1"
                     /translation="QNEDAAYAAMNHYISPQTQSFYAETYTYLVSDQRTLDELKPALV
                     EELGLDDPSQLDAAIALQLPENYDLWLDAYREFKAA"
BASE COUNT          540 a          995 c          975 g          464 t
ORIGIN      
        1 cgagcgatgt cccccttcga tcgacacctc ggcgccgtcg gcccccacgg actcgacccg
       61 cccggacagg aagctggttt gcccgatgaa gcccgcgacg aacaacgacg ccgggctcag
      121 gtaaacctcc tcgggcgtac cgaggtgttc caccacccca ccgttcatga cggcgatgcg
      181 atcgctcatc gtcaaggcct cgccctggtc gtgggtgacg tacacgaagc tgatcccgac
      241 ctcgcgctgg atgcgtttga gctcgaactg catctcctcg cgcagcttga ggtcgagggc
      301 accgagcggc tcgtccaaca gcagcaccga cggttggttc gccagcgctc gagcgagcgc
      361 aacgcgttgc gcctgaccgc ccgagagctc acgtgggtac cgcttgccat agccctcgag
      421 ccgcaccgcc gacagagact cctgcacgcg ctgttcgacc tctttgcggg cgagcttctt
      481 gcgctgcggg ccgaacgcca cgttctgggc cacggtcatg tgcgggaaca gggcgtagga
      541 ctggaacacc atgttcgtgt cgcgttcgtg gggcgcgagg cgcgacgcgt ctcgtccgtc
      601 caccaggatc cggccctcgg tcggggtctc gaacccggcg agcatccgca aggtcgtggt
      661 cttgccacat ccgctcggcc ccagcagcga gaagaactcc cggcgaccca catcgaagct
      721 gaggccgtcg acggccacga cctcgccgaa gcgcttcacc acgtggtcga gggacaacgc
      781 gacgccgtcg ccggcgccgt cttggacgac ggccgcatcc cctccgacgt gctcggtctc
      841 ggtgctcacg ggttcctcct catgacgtcg cctgcagacg acggatcttc gccatccggt
      901 agccgatcca cgccaccgcg ccggacaaca ccagcaacat gaagctcatc gccgacccca
      961 cgccgcggtt gcccgattcc aggatcgctt cctggatcgc gttgccgatc atgtagccgc
     1021 tggtgccgcc caccagcgcc ggcgccgcga actccgaata gagcgggatg tacaccagga
     1081 tgatcgcgac gaagatcccg ggcgccgcca gcggcaggag gatcttgcgg catcgcgtga
     1141 agaaggacgc gcccaggtca tcggcggcct cgagcaggtc ggggtcgacc gccttcagcg
     1201 cggcgtagat cgggatggtg gtgaacgaga tgtaggtcgt ggacagcacg atcaccacgg
     1261 cggtcgagct gaacaacagc gagtcgatcg gtttgtcgat cagcccgatc gtctgcagcg
     1321 tccagttgat caccccgccg cgtcccagga tcatgcgcca ggcgtagatc cggatcagcg
     1381 ggttgagctc ctcggcgatc accagcatca gcaggagcag cagttcgaac cgcggcgggg
     1441 tccgcatggc caagatgtag gcgagcggca ggccgatcgc gagcatgatc agcatcgaga
     1501 tcgtgcagat catcaccgtg gtcagcatga ccttgccgta cgtgggatcg gtgaggatcg
     1561 aggtgtagtt gtcgaacgtc cagccgccgg cgcccgcgag cgaggccttc tggaagctgt
     1621 aatacagcag gatgaacatc ggtcccagga acagcgccag cagcaccatg gtgggcgcga
     1681 tcgaatagat cggaccggcc cacctgccct tggggcgggc gcgcgtggtg tcctcggtgg
     1741 acggcgagac cgggggcgcg gtcgctacca cagcatgtcc atcgggtcgt cgttggcggc
     1801 catcttcttc gagcggcggc gccacagccg ccacgctaca tacacgatca gcagctgggc
     1861 cagtgagacc agcgtcgaga tcgcgttcac gatgggcacg ttctgtgcgt gccgcaacgt
     1921 ggagaacacc cagatcggga aggtcagctc gaacccaccc acgaagaagc tgatcacgaa
     1981 gttgttgaac gagtagctga aggccaggat cgccgccgcg gcgagcgccg gcaccaggtg
     2041 gggcagcgtg atgtagcgga gcacctcgcg gttcgtggcg cccaggtcac gggccgcttc
     2101 ctccatccga tgatcgaagc ggatgagccg agccgtcacg atggcggcca cgagcgggaa
     2161 cgccaccacg atgtgcatca ccgcgatcgt ccgcaggttc agcggcacct tcaacttcga
     2221 gaagaacagg agcgcaccga cggccaggat cagccagggc acgatcagcg gaccggcgcc
     2281 cagcgccgcg acgctacccc ggcccttgta gcggaaccgt gtggtcgcga acgccgccat
     2341 cgtccccagg atcaacgcga ccggggtgac gacgagcgcg acggccaggg agttcttgag
     2401 cgactcacgc acgagcgggt tctggagcgc ctcgcggtac cactccaggg tgaagccctt
     2461 gaaaggcagc gcgatcacga tcgagtcgtt gaacgagaac agcgccagga tcaacaacgg
     2521 gccgtagagg atcaccacca cgatcgccag gaacacccag cgggtgatgg ccgcggcgcg
     2581 cgctcgtccc tcgcggccgc ccaccgagaa gccacgaccg cgttggtcgc cggccgtggc
     2641 ctccggctcg atcttcgtcg tggtggcgct caccgagccc ccgtcatgcc gactcgatct
     2701 ggcgcatcgc gtccagccga tcgccgtgcg ctcacgctgc tttgaactct cggtaggcgt
     2761 cgagccacag gtcgtagttc tccggcagtt gcagggcgat cgcggcatcc agctggctgg
     2821 gatcgtccag gcccaactcc tccaccaggg ccggcttcag ctcgtcgagc gtccgctgat
     2881 ccgaaacgag gtaggtgtag gtctcggcgt agaacgactg cgtttgcgga gagatgtagt
     2941 ggttcatcgc ggcgtaggcc gcgtcctcgt tctg
//
LOCUS       DATDBZ010000184         2981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1494562, whole genome shotgun sequence.
ACCESSION   DATDBZ010000184 DATDBZ010000000
VERSION     DATDBZ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494562"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VIX62_04490"
     CDS             complement(<1..339)
                     /locus_tag="VIX62_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY5859522.1"
                     /translation="MTYQLAPNIELLFTEAGDYHDRVRAAAAAGFAAVEMWGPTGVDA
                     PATPKDLPALKAALDETGTQLTAQLAEPRTQFMIPPWDHSEFYRKLDEGVEIAHFLGT
                     PRMVVGSGTGF"
     gene            complement(357..740)
                     /locus_tag="VIX62_04495"
     CDS             complement(357..740)
                     /locus_tag="VIX62_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5859523.1"
                     /translation="MTTVATDNRVAFERFVDLFYTQKRVAEAFADVVSDDYRQHNPTI
                     PDGPAPAIEMLTPKFDGSPEARFEIQRIIVDGDLAMVHVKASRPGAPDAAVADIYRFE
                     DGRIVEHWDVLQQVPVHAAHDHPMF"
     gene            complement(741..1577)
                     /locus_tag="VIX62_04500"
     CDS             complement(741..1577)
                     /locus_tag="VIX62_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY5859524.1"
                     /translation="MFRYTKATAVREVLVWVVTLIGLLPFYLLVVTAFKTDQEVLTTS
                     AVAPPSSIDFSAFIEVLTTTGRNSIPMSILNSVIITAGAIFGLVLLGSVSAYVIARRT
                     RAWTNLTYYLVLIAIILPAQLGTVPLYIGARSVGLTGNALGMILLWIGILLPLSVFLY
                     ASFFRGLTTEYEEAAVIDGASPTQAFFRVVLPLMAPATGTVAILAGLIVWNDFFNSLI
                     FLGGSETQTLPVAMYTYVGGLVSAWNKIFAVVIISMVPILLFYMFAQKRFIQGFAGGL
                     KG"
     gene            complement(1582..2544)
                     /locus_tag="VIX62_04505"
     CDS             complement(1582..2544)
                     /locus_tag="VIX62_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY5859525.1"
                     /translation="MTVTIDESSTRTLTVPPEAWNTPAPRKRGKKPLISYGHWWWALP
                     AVILVIAVHYVATLTGGFFAFTDWTGLGDWSFIGLDNFARIFQDPLMLGAVGNTLFLA
                     FASVILTNVFGLLYALAINRMLKSRYILRTLLFMPVVLSPLAVAYVWKFIFQFDGPLN
                     GFLGLIGLEELQKVWLADPTWSIWAILITVVWQQTGFTMVIYLAGLASVPIEVEEAAA
                     LDGAGIWGRFRYVIVPAIRPSIAIATTLGIIQGLRIFDQILALTGGGPAGATETLATQ
                     VYKQAFSLGQFGFGSALALVLTVIILAFAILQQYVTRDRDARKA"
     gene            2703..>2981
                     /locus_tag="VIX62_04510"
     CDS             2703..>2981
                     /locus_tag="VIX62_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY5859526.1"
                     /translation="MNEHQSSPPDAGPAAETDPVWRAFIDPPDEARPRAWWHWMDGNV
                     DPAGIVRDLRWLHDVGVRGVQVFDGGMGGPLVVPAAVRPGSEAWDEAVD"
BASE COUNT          532 a          905 c         1069 g          475 t
ORIGIN      
        1 gaatcctgtg ccactgccga ccaccatgcg gggcgtcccg aggaagtgcg cgatctcgac
       61 gccctcgtcg agcttgcggt agaactcgga gtggtcccac ggcgggatca tgaactgcgt
      121 gcgcggctcg gcgagctgcg cggtcagctg agtgccggtc tcatcgagcg cggccttgag
      181 cgcgggcagg tccttgggcg tcgcgggggc atcgaccccg gtcgggcccc acatctcgac
      241 ggcggcgaag ccggctgccg cggctgcgcg gacgcggtcg tggtagtcgc ccgcctcggt
      301 gaagaggagt tcgatgttgg gtgcgagctg gtaggtcatg agtcgtcctt cggagatcag
      361 aacatcggat ggtcgtgggc ggcatgcact ggaacctgct gaagcacgtc ccagtgctcg
      421 acgatccggc cgtcctcgaa tcggtagatg tccgcaactg cggcgtcggg tgcgccgggt
      481 cgggaagctt tcacgtgcac cattgccagg tcgccgtcga cgatgatccg ctggatctca
      541 aatcgcgcct cgggtgagcc gtcgaacttc ggggtcagca tctcgatcgc gggcgcgggt
      601 ccgtcgggaa tggtcgggtt gtgctgccga tagtcatcgg agaccacgtc ggcgaaggct
      661 tccgcgaccc gcttctgcgt gtagaagaga tcgacgaacc gctcgaacgc gacccggttg
      721 tcggtggcca cggtcgtcac tcagcccttc aggccgccgg cgaagccctg gatgaagcgc
      781 ttctgcgcga acatgtagaa cagcaggatc ggcaccatcg agatgatcac gacggcgaag
      841 atcttgttcc acgccgagac caggccaccg acgtaggtgt acatcgcgac cgggagcgtc
      901 tgggtctcgg atccgccgag gaagatcagc gagttgaaga agtcgttcca cacgatcagg
      961 ccggcgagga tcgcgaccgt tccggtcgcg ggggccatca acggcagcac cacccggaag
     1021 aacgcctgcg tcggcgaagc gccgtcgatg accgccgcct cctcgtactc ggtcgtcaac
     1081 ccgcggaaga agctcgcgta gaggaagacc gagagtggga gcaggatgcc gatccacagc
     1141 aggatcatcc cgagggcgtt tccggtgagc cccaccgagc gcgcaccgat gtagagcggc
     1201 acggtgccca gctgcgcggg caggatgatc gcgatcagca cgaggtagta ggtgaggttg
     1261 gtccaggccc gcgtgcgacg ggcgatcacg tacgccgaga ccgaaccgag cagcacgagg
     1321 ccgaagatcg cccctgcggt gatgatgacg ctgttgagga tgctcatcgg gatgctgttg
     1381 cgcccggtcg tcgtgagcac ctcgatgaac gcactgaagt cgatcgacga gggcggggcg
     1441 acggcgctgg tggtgagcac ctcctggtcg gtcttgaacg cggtcacgac gaggaggtag
     1501 aagggcagga gcccgatgag ggtgacgacc cagacgagca cttcccggac ggcggtggcc
     1561 ttggtgtagc ggaacatgag atcaggcctt tcgtgcgtcg cggtcgcggg tgacgtactg
     1621 ctggaggatg gcgaacgcga ggatgatcac ggtgaggacg agcgcgagcg ccgagccgaa
     1681 gccgaactgc cccagggaga acgcctgctt gtagacctgg gtcgcgagcg tctcggtcgc
     1741 gccggccggt ccgccgcccg tgagggcgag gatctggtcg aagatacgta gcccttggat
     1801 gatgccgagc gtggtcgcga tggcgatcga cgggcggatg gccgggacga tgacgtagcg
     1861 gaaccggccc cagatcccgg cgccgtccaa ggcagcggcc tcctcgacct cgatcggcac
     1921 gctggccagt ccggcaaggt agatgaccat ggtgaatccc gtctgctgcc agacgacggt
     1981 gatgaggatg gcccagatcg accaggtcgg gtcggcgagc cacaccttct gcagttcctc
     2041 gagcccgatc aggccgagga agccgttgag gggaccgtcg aactggaaga tgaacttcca
     2101 gacgtaggcg acggcgaggg ggctcagcac caccggcatg aacagcagtg tccgcaggat
     2161 gtaccgcgac ttgagcatgc ggttgatcgc cagcgcgtag agcaggccga acacgttggt
     2221 gaggatgacg gaggcgaacg cgaggaacag cgtgttcccc acggctccga gcatcagggg
     2281 gtcctggaag atccgcgcga agttgtcgag tccgatgaac gaccagtcgc cgaggccggt
     2341 ccagtcggtg aaggcgaaga agccgccggt cagcgtcgcg acgtagtgca ccgcgatgac
     2401 caggatgacg gcggggaggg cccaccacca gtggccgtag ctgatgagcg gcttcttccc
     2461 gcgcttccgc ggcgccggcg tgttccacgc ctcgggcggc acggtgagtg tgcgagtgct
     2521 gctctcgtcg atcgtcactg tcatctgctc tctgacctcg tcgtcacgca ccccgctgtg
     2581 cgatggggcg ccctacggca actctggcac acttgcgtcg gaaagacaca ttagtgttcc
     2641 caatatcgcg tataagtagt ggcaccatcc gtttcgggca ccgactggga ggatgacacc
     2701 ctgtgaatga gcaccagtcg tcaccgcccg acgcggggcc cgcggccgag accgacccgg
     2761 tctggcgagc cttcatcgat cccccagacg aggcccgccc cagagcctgg tggcactgga
     2821 tggacgggaa tgtcgatccc gccggtatcg tgcgcgacct ccgatggctc cacgacgtcg
     2881 gcgtgcgcgg cgtgcaggtc ttcgacggcg ggatgggcgg accgctggtg gtccccgctg
     2941 cggttcggcc cgggtccgag gcctgggacg aggccgtgga c
//
LOCUS       DATDBZ010000185         3120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_882167, whole genome shotgun sequence.
ACCESSION   DATDBZ010000185 DATDBZ010000000
VERSION     DATDBZ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882167"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /gene="mptB"
                     /locus_tag="VIX62_04515"
     CDS             complement(<1..1549)
                     /gene="mptB"
                     /locus_tag="VIX62_04515"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HEY5859527.1"
                     /translation="MSRLDLLRYPLASWLTAKRAVTASVASMTAVMLIAATPGSPYHP
                     VLPESQANGPLELLSKAVLLDRLPHGLLIAIGFVAMIAAGVSFLLILWAAERGLVSTK
                     TVLTLTVAYHLAILLLPLLLSRDVFSYAYYGRILSRYGGNPYVNTPADYPANDLWRFT
                     WPGWRETPSVYGPVFVWLAAAITAIFRSIPDVIMAFRAVAVAASLGAVWFVVKLVQRV
                     KPSKTAYAAAMIGLNPVVLFHTVGGGHVDVLVMLSVAAGIYLVATDRELPATVALTVG
                     ALVKISAAVPLVLLIAYVATRAEPGKRARVLVTHIGTAVGIFMVAALPFLQRSNPTLG
                     MVELVQHGSWIAPPALVERVFEALGELLAGEVGASIGVIIARLGMFAALAVGLFLVLR
                     QVVRHASEGSVSFLAAAWGWGFLLMMLFSPTLFPWYFAWVLPVAWALPLVPRRTLELS
                     FLALVTASLTTENFQLPAWMHVDLAIGHPVLVIVLVWFLHDLWLRLKYDVPLDAETDR
                     ARELHRER"
     gene            complement(1574..2341)
                     /locus_tag="VIX62_04520"
     CDS             complement(1574..2341)
                     /locus_tag="VIX62_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY5859528.1"
                     /translation="MTLADHDLATGVGVLDRSMAILDAVQAGSRTHASIVRATGFPRT
                     TTHRLLKSMEAHGLLEFVGGYGYRLGPRLLKIAASALQEPSLRDIGHPALERLASNTG
                     ESAQLFVAALGERVCVDSVESSSELRTFVKVGAILPMWAGSAGKVFLAWMSPNARQEL
                     MTNARSLTPATPTRERLIQQLATVKARGWASSAGERQDGVGSVSAPVFGPVGELVAAV
                     SISGPISRVSRIGAKRYAPAVMAAAREVEAAIGGTRT"
     gene            2440..>3120
                     /locus_tag="VIX62_04525"
     CDS             2440..>3120
                     /locus_tag="VIX62_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY5859529.1"
                     /translation="MASQTDTPRTLAEKVWDRHVVHSADGEPDILYVDLHLVHEVTSP
                     QAFESLRLHGRPVRRPDLTVATMDHNVPTTQGPVTDTISARQMEALATNAKDFDVTLY
                     PMGSPGQGIVHVIGPEQGNTQPGLVIVCGDSHTSTHGAFGALAIGIGTSEVEHVLATQ
                     TLPQNRPGTMSVTVDGDLPASCSAKDIILAIINRIGTGGGVGHIIEYRGSAIRALSME
                     GRMTICNMS"
BASE COUNT          580 a         1086 c         1029 g          425 t
ORIGIN      
        1 ggcgttcccg atgcagctcg cgcgcacggt cggtctctgc gtcgagcggc acgtcgtact
       61 tcaagcgcag ccacaggtcg tgcaggaacc acacgagcac gatgacgagc accgggtgcc
      121 cgatcgccag gtccacgtgc atccaggcgg ggagctggaa gttctctgtg gtgagcgagg
      181 ccgtcaccaa cgccaggaac gacagttcca gcgtgcggcg cggcaccaat ggcaacgccc
      241 acgccaccgg cagcacccac gcgaagtacc aggggaacaa cgtgggcgag aacagcatca
      301 tcagcaggaa gccccagccc cacgccgccg ccaggaacga gaccgaaccc tcggaggcgt
      361 gacgcaccac ctgccgcaac accaggaaga gtccgaccgc caacgcggcg aacatgccca
      421 accgcgcgat gatgacgccg atcgatgcgc cgacctctcc ggccaggagt tcgcccaagg
      481 cttcgaagac gcgctcgacc aacgccgggg gcgcgatcca cgaaccgtgt tgcacgagct
      541 cgaccatgcc gagcgtcggg ttcgagcgct gcaggaacgg caacgccgcc accatgaaga
      601 tgcccaccgc cgtaccgatg tgggtgacca ggacgcgggc gcgcttgccg ggctcggctc
      661 gcgtcgcgac gtacgcgatc aacagcacca acggcacggc ggccgagatc ttcaccagcg
      721 cgccgaccgt caacgccacg gtggctggga gttcccgatc ggtggcgacg aggtagatcc
      781 ccgcggcgac cgacagcatc accagcacgt cgacgtgccc cccgcccacg gtgtggaaca
      841 gcacgaccgg gttgagcccg atcatcgccg cggcgtacgc ggtcttcgag ggcttcacgc
      901 gctgcaccag cttcacgacg aaccacaccg cgccaaggct cgcggccacc gccaccgcgc
      961 ggaacgccat gatcacgtcg gggatcgaac ggaagatcgc ggtgatcgcg gcggcgagcc
     1021 acacgaacac cggtccgtag accgaagggg tctcacgcca ccccggccag gtgaaccgcc
     1081 acaggtcgtt cgcggggtag tcggccggcg tgttgacgta ggggttgccg ccgtagcgcg
     1141 acaggatgcg gccgtagtag gcgtagctga agacgtcgcg ggagagcagc agcggcagca
     1201 gcaagatcgc gaggtggtag gccacggtca gcgtcagcac ggtcttggtg gagaccagac
     1261 cgcgttcggc agcccacagg atcagcagga agctcacgcc ggcggcgatc atcgcgacga
     1321 acccgatcgc gatcagcagc ccatggggga gccggtccag gagcacggcc ttcgacagca
     1381 gttccagtgg tccgttcgcc tggctttcgg gcaggacggg gtggtacggc gaacccggcg
     1441 tcgccgcgat gagcatgacc gcggtcatgc tggccacgga cgcggtgacg gcacgcttcg
     1501 cggtcagcca ggaggccaac gggtaccgaa ggagatcgag ccgggacatt cggacgagct
     1561 tacgggaagc gggtcaggtg cgcgttccgc cgatggcggc ttcgacctcg cgcgcagcgg
     1621 ccatgaccgc cggcgcgtac cgcttcgcgc cgatgcggct gacccgtgag atcggccctg
     1681 agatcgatac cgccgcgacg agttccccga cgggtccgaa gaccggagcg ctcaccgaac
     1741 cgacgccgtc ttgtcgctcc ccggccgacg atgcccatcc gcgagctttg acggtggcga
     1801 gctgctggat cagccgctcc ctggtcggtg tcgcgggagt aagcgatcgg gcgttcgtca
     1861 tgagctcttg cctggcgttg ggcgacatcc atgccaggaa gaccttcccg gccgatccgg
     1921 cccacatcgg gaggatcgcg ccgaccttga cgaaggtcct gagctcgctc gaagactcca
     1981 ccgaatccac acacacgcgc tctccgagcg cggccacgaa cagctgcgcg ctctcgccgg
     2041 tgttcgaggc caggcgttcc aacgccggat gaccgatgtc gcggagggag ggttcctgca
     2101 gtgccgacgc ggcgatcttg agcagccgtg gccccagccg gtagccgtac ccgccgacga
     2161 actcgaggag gccgtgcgcc tccatcgatt tcaacaaccg gtgggtggtc gtccggggga
     2221 atccggtcgc tcgcacgatc gatgcgtggg ttcgggagcc agcctgcacg gcgtcgagga
     2281 tcgccatcga tcggtcgagg acaccgacgc cggttgctag gtcgtggtct gctagggtca
     2341 tgttccacat atcggtatca cagttccact attcggaaca agctgagcgg cgggaaggcc
     2401 ctgactcggc cccgaacgcg aggcaccacc ggaggagcca tggcatcgca gaccgatacc
     2461 ccgaggaccc tggctgagaa ggtctgggac cgccacgtcg ttcacagcgc cgacggcgaa
     2521 cccgacatcc tctacgtcga cctccacctc gtgcacgagg tcaccagtcc gcaggccttc
     2581 gagagccttc ggttgcacgg acgccccgtc cgccggcccg atctcaccgt ggccacgatg
     2641 gaccacaacg tccccaccac ccagggtccg gtcaccgaca ccatcagtgc ccgccagatg
     2701 gaagccctcg ccaccaacgc gaaggacttc gacgtcacgc tctatcccat gggctcgccc
     2761 ggccagggca tcgtgcatgt gatcggtcct gagcagggga acacgcagcc gggcctggtg
     2821 atcgtctgcg gcgactcgca cacctcgacg cacggagcgt tcggcgcgct cgcgatcggc
     2881 atcggcacca gcgaggtcga gcacgtcctc gccacccaga ccctgccgca gaaccgtccc
     2941 ggcacgatgt cggtcaccgt cgacggcgac ctgcccgcct cgtgcagcgc caaggacatc
     3001 atcctggcga tcatcaaccg catcgggacg ggcggcggcg tcggccacat catcgagtac
     3061 cgcggttccg cgatccgtgc cctctcgatg gagggccgca tgacgatctg caacatgtcg
//
LOCUS       DATDBZ010000186        13349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1494906, whole genome shotgun sequence.
ACCESSION   DATDBZ010000186 DATDBZ010000000
VERSION     DATDBZ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13349
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494906"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VIX62_04530"
     CDS             complement(<1..647)
                     /locus_tag="VIX62_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY5859530.1"
                     /translation="MFTVEQRDALLARVLGLGEQDERVVAGAVVGSLAFDEGDRFSDV
                     DLTFGVADHVEVAAVLGDWTRTLVDEFDAVPLVDLERGPITYRVFLLPDALQLDLSMS
                     EAAGFRPAGSRFRLVFGETAADRPEASAPPVVGDLFIATPSIPEDVFGWGVIYAVHAR
                     ACIERGRVWQAEHYVGAVRDHALAIACLREGLGAAQARSYDDLSAESLARFDRTHV"
     gene            728..1873
                     /locus_tag="VIX62_04535"
     CDS             728..1873
                     /locus_tag="VIX62_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859531.1"
                     /translation="MRRFLLRGGLRVLAAATLLACGMPPTGSLAATDFTSIWAGVDHT
                     CAVTPAGAAQCWGKNNAGQSEDRPGPFSQVSAGGLHNCALTTGGAAECWGDNGYGESD
                     DHAGPYVQVVAGWMHTCGLTTAGAADCWGRNDYGQAEDQPGPYTELTMSGAHNCGLTP
                     GGVADCWGRNNAGLADDHPGPYMQVSAGDGHNCALTTNGAADCWGLPDDGQANDRPGP
                     FTQLSAGDHHNCGLRLDGSVRCWGHNDFGQAADRLGPYVEVSAGSYHTCATTAAGDTD
                     CWGDNRDGQLGRRLTISAPGAVTAGSRARITGRLQSIDPACIASQTVALRVLRPAGVP
                     SRTTMTSTAGRYTFNVKITRETVVRVEFAPAGSCERVRSTRRTIVAT"
     gene            complement(1910..2287)
                     /locus_tag="VIX62_04540"
     CDS             complement(1910..2287)
                     /locus_tag="VIX62_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010442807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5859532.1"
                     /translation="MERVDGIGGFFFRAADPDGLAAWYEQHLGIDPVPTSYGATVWTQ
                     REGPTVFAPFGPDAAESPVIGRGGWGVNFRVRDLDAIVAQLGSAGVDVEVDETDYPNG
                     RFAQLNDPEGNAIQLWEPKEPIG"
     gene            complement(2340..2413)
                     /locus_tag="VIX62_04545"
     tRNA            complement(2340..2413)
                     /locus_tag="VIX62_04545"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Gln,seq:ttg)
     gene            complement(2596..3471)
                     /gene="ispE"
                     /locus_tag="VIX62_04550"
     CDS             complement(2596..3471)
                     /gene="ispE"
                     /locus_tag="VIX62_04550"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY5859533.1"
                     /translation="MSTEPITRRAHAKINVFLRVLGRREDGFHELESVVLPLSLHDLV
                     TVSPDGGSDPSVTVEGVPELTGRVPTGEDNLVIAAVRELSKRSGIPGGFRVDIHKRIP
                     VAAGLGGGSADAAATLLGLADLLAEVDGGHDDPASLTSIATELGSDVPAMLAGGPVMV
                     TGRGEVLTPVHTVTTWWVLKPFDVAVTAADAYGWWDHDPTTGPDPGVLIAALETGDLA
                     LLGSALFNDLQAPVVARHPEVGEAIDAFMEAGALGAIMSGSGPTVAALASHMGHADRL
                     AEAVGGAIVVSGPPA"
     gene            complement(3468..4295)
                     /gene="rsmA"
                     /locus_tag="VIX62_04555"
     CDS             complement(3468..4295)
                     /gene="rsmA"
                     /locus_tag="VIX62_04555"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY5859534.1"
                     /translation="MGAGALRAVARRHGIRPTKSLGQNFLIDPNLARAIAAEAGVGPG
                     DRVVEVGAGLGSLTMALAATGAQVLAIEFDRALIPALTEVVGSAGNVRILHADATKLD
                     WASTLGDAGAWTLCANLPYNVAVPVVMEVLASAPAVRRLVVMVQREVGERLAATSGDQ
                     HYGAVSIKVAYRARATITRRVPSTVFWPKPSVDSVLVRLDRLSSPAVDVDAASLWRVV
                     DGAFAERRKTMRNALRRLDLPATEADAILIATGVSASARPEELDLETFAAIASAVPA"
     gene            complement(4330..5409)
                     /locus_tag="VIX62_04560"
     CDS             complement(4330..5409)
                     /locus_tag="VIX62_04560"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEY5859535.1"
                     /translation="MVGLLLLSGVVHFALRKHVTLVVDGKSQAVQTTSSNVHELLAGE
                     GISLTSSVQVDPPPATELADGMTVVVSPAPAAPGVRDLIASDATTVYQSPTDVGVWVM
                     AGTNSNIAGLANELVESSFSASSVGSSPVVSVRAVVLGKVRDVLTNAGSTGELLSAMG
                     INADADDRVTPSPSTPLQSGATLTYDQVQVTTELEDETIPFDVHTRFTPSMVPGNVKV
                     LQKGQDGRAVGTYRVTVVNGQEVERALIARWIERDPVDEERLSAPESMYGGTTEVPGT
                     DGASQTGLASWYDPPWSGLTAAHPSLPFGTMVTVTDVETGRSVTVVIDDRGPFAPGKI
                     IDLSPEAFAVLRPLGTGVIDVQLSW"
     gene            complement(5611..6405)
                     /locus_tag="VIX62_04565"
     CDS             complement(5611..6405)
                     /locus_tag="VIX62_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY5859536.1"
                     /translation="MSDQNLQPGTDTGAVDTHCHLFLLDHEPAQVVETAKAAGVDRLI
                     CVGVDPATSARSAELADSLEGVFATAGMHPHDASDFDQAASARIEELLHDARVIGVGE
                     CGLDYFRMHSPAEDQIRVLQTHIALSNETGKPMVVHVRDAWPDVLRVLDEGSAERVVI
                     HCFSGDAAIARECAARGYWISFAGNVTYPKNEHLRQAAVAVDLDRIVVETDSPFLSPQ
                     KLRGSNNEPSNVMLTIAEIARVRDDDVEKIVTATAENARSAFPGLR"
     gene            complement(6542..8086)
                     /gene="metG"
                     /locus_tag="VIX62_04570"
     CDS             complement(6542..8086)
                     /gene="metG"
                     /locus_tag="VIX62_04570"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY5859537.1"
                     /translation="MGRDVFYITTPIYYPNDVPHIGHAYNAVANDFIARYHRLRGEEV
                     FHLTGTDEHGLKIQRAAEGAGVSPQQWVDDMEPKWREVWARLDIGYDDYLRTTQPRHE
                     HAVAALLNAVHDNGRDDIYLGSYEGLYCVSCEAYYSEDELLEGGLCPIHETPVEHLVE
                     ENYFFRLSAYADRLLEHYEQHPSAVEPETRRNEVLSLIRGGLQDFSISRTSFSWGVPI
                     PWDPKHICYVWFDALTNYIAAAGYASDPDRFARVWPANIHLIGKDILRFHAIYWPAML
                     MAGGVEPPTQVWAHGFLTVGGKKMSKTNLTGIHPFELLDQFGIDSYRYYFMREMRFGE
                     DGNFSFESMVERHNADLANGLGNLASRVLAMLGSYFDGTVPAPVVEGAESDLPSVVAD
                     AVARYDDHMLAVHLQPALIAVWDIVDRANHYLVETEPWKLAKDEGRREELASVLYASA
                     ETLRILAVLIFPIMPAAAGRLWSQLGQTASLEGQRVPASVEWGGLEVGTSTTKGESLF
                     PRLDAD"
     gene            complement(8152..9783)
                     /locus_tag="VIX62_04575"
     CDS             complement(8152..9783)
                     /locus_tag="VIX62_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY5859538.1"
                     /translation="MDERFAREMAAGYAFDEPCVVLGGPMLDGEVDLGSRVQLPLSMV
                     NRHGLVAGATGTGKTKTLQILAGQLSDAGVPVFIADVKGDLTGISQPGDAAAAPVAER
                     CAQLGWTFEPKAHPVELLSLSGDLGAQVRATIHSFGPLLLAKVLDLNKTQTSVLSLVF
                     KYCDDENYPLLDLADLRTTLKFLSSDDGKPVLEEYGGVSKASVGVLLRSLVELEEAGA
                     GDFFGEPQFDVVDLLRITPDGKGVVSLLEISDVMDRPRLFSTFMLWMLAQVYAELPEE
                     GDLPKPKLAFFFDEAHLLFDDASEALLDQIELTARLIRSKGVGVYFVTQSPTDVPSSV
                     LSQLGNRVQHALRSFTPDDETALNKTVKTFPKTEHYDVGATLTSLGIGEALVTLLTPK
                     GVPTPLAATRLVPPDSLMDAVDETSFQRLLADGALGAKYAQAVDRESAHEMISARLGT
                     AKEAAGVPAAPAGSVPLPPPPPGSPPASPTPAPKPRPKAESKPKATSGSKPKESDGVG
                     DQVKGVAVDFAKDMLTTADGRKTLRSVFGTLFGKQ"
     gene            complement(9820..10644)
                     /gene="rsmI"
                     /locus_tag="VIX62_04580"
     CDS             complement(9820..10644)
                     /gene="rsmI"
                     /locus_tag="VIX62_04580"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY5859539.1"
                     /translation="MEGTLWLVGTPIGNLGDLAPRAREVLADVDLVACEDTRRTGRLL
                     HGLSLRKPMVSFFEGNERERTDELIERLRSGSSVALVTDGGMPSVSDPGYRLVHGAIE
                     AGIDVKVVPGPSAALAALVLSGLPTDRFTFEGFLPRKPGERMRRLDALRHDPRTLVVF
                     ESPRRVQILLRDALVALGDRRIAIARELTKLHEEVMRGRISEVLSHIGDAELKGEVVV
                     VIDGEHTPETPDLETCADEAQELVDEGMRKRDAAHAVAERHHVSANDVYRQLLKRG"
     gene            10711..11145
                     /locus_tag="VIX62_04585"
     CDS             10711..11145
                     /locus_tag="VIX62_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEY5859540.1"
                     /translation="MADGHKDYSTTPLWRKLGIREGSRVRLLNAPDGFEDALVAIAPL
                     PNAVDFLRRSAKGIDVAVLFTTERQVLERRFGALATTLEPDGRLWVAWPKKASRVATD
                     LTFEIVQSHGLAGGMVDNKSAAIDEVFQGLQFVYRVQDRPGR"
     gene            11191..12759
                     /locus_tag="VIX62_04590"
     CDS             11191..12759
                     /locus_tag="VIX62_04590"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY5859541.1"
                     /translation="MSALLQRLNRPIVAILAVAALAGIARGWALANPPDLVFDEVYYA
                     KAGCIFVGGSDQTCRIDEDAEHYWVEHKWDVGSWVHPPLGKWMTGMGIKAFGMGAFGW
                     RFPSAVAGTLIAVMVAVIAQLLFGRPLWTFVAGGLIALDGLNVVMSRVAMLDIHLAFW
                     AVLGFLCLVLDRRWIDRRTERALADEPVQTVTVVGEDGATVLVEGRAGAAGRGRTRVP
                     SPLWRPWRFASGAAMGAACAVKWSGLTALAAAVLVTVVWETTRRHVPGVSRWRAFWRT
                     VGMELLGLILAFLVVPAIVYMATWLPWFNHFGWSLSAWWENQKEMWRYHANLTEFAYD
                     SKTDSFTPTHGSYSKAWSWILMKRPVNFFVENTTGHVRQILTIGNLALFWGTVFAIPY
                     AVWSWARTRDWRAGFVVMPFLVMWLPWFVVSRPQFFFYVLPMTPFMALAAVYTLQALS
                     DARLVLHDEAGEVAIDPETGRPAISTRHPFRPVVMGYLAIFVVLFVWFWPLLVGERLT
                     TSFRALHIWMRSWN"
     gene            12826..>13349
                     /locus_tag="VIX62_04595"
     CDS             12826..>13349
                     /locus_tag="VIX62_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY5859542.1"
                     /translation="MTRREHVWIPMDDGVRLAATLYLPDPADGGDGPWPALLEALPYR
                     KDDVTASYRPEYQRLAEAGYVMCRVDVRGTGTSEGIAEDEYPAIERTDLVTVIDWLAT
                     RDWSTGSVGMYGTSYSGFNSIQLAMERPPALKAIIPIFATDDRFGDDVHYFGGALKAL
                     DLVDYPTYMVAMNAL"
BASE COUNT         2063 a         4539 c         4649 g         2098 t
ORIGIN      
        1 acgtgggttc ggtcgaaacg agccagggac tccgcggaga gatcgtcgta gctgcgcgcc
       61 tgcgccgccc ccagcccttc gcggaggcag gcgatggcga gagcgtgatc gcggacggca
      121 ccaacgtagt gctccgcctg ccacacgcgc ccgcgttcga tgcaggcgcg ggcatgcacc
      181 gcgtagatga cgccccaccc gaagacgtct tcagggatcg acggcgtggc gataaagagg
      241 tcgccgacga ccggcggcgc ggacgcctcg ggcctatcgg cagccgtctc gccgaacacg
      301 agccggaacc gcgagccggc gggacggaac cccgccgcct cactcatcga caggtccaac
      361 tgcagcgcgt ccggcaacag gaacacccgg taggtgatcg gcccgcgctc gagatcgacg
      421 agcggtactg cgtcgaactc gtcgacgagg gttcgcgtcc agtcgccaag gacagcagcg
      481 acctccacgt gatcggcaac gccgaacgtc aggtccacgt cggagaagcg gtcgccctca
      541 tcgaacgcga gcgagcccac gacggccccg gcgacgacgc gctcgtcctg ctccccgagg
      601 cccaggacgc gggcaaggag tgcatcccgc tgttcaaccg tgaacatgga cacgatgcta
      661 gctccgcatc ggccgcgctc ggggcgagcg gcaccatcag agggtcatcg gggactacgc
      721 tcggatgatg cggcgcttcc tgctccgagg cgggctgcgc gtgttggcgg ccgccacgct
      781 cctcgcgtgt gggatgcctc cgaccggcag cctcgcagcc accgacttca cgtcgatctg
      841 ggcgggcgtc gaccacacct gcgccgtcac gcccgccggc gccgcccagt gctgggggaa
      901 aaacaacgca ggccagtccg aggatcggcc gggaccgttc tcccaggttt ccgccggtgg
      961 gctccacaac tgcgcgctca ccacgggcgg agcggccgag tgctggggtg acaacggata
     1021 cggcgagtcc gacgaccacg ccggccccta cgtgcaggtg gtagcgggct ggatgcacac
     1081 ctgcgggttg acgaccgccg gcgctgccga ctgctggggt cgcaacgact acgggcaggc
     1141 ggaggatcaa ccggggccgt acaccgaact gacgatgagc ggcgcacaca actgcggact
     1201 gacgcccggc ggcgtcgcgg actgctgggg tcgcaacaac gccgggctcg ccgacgacca
     1261 cccgggcccg tacatgcagg tctcggcggg cgacggtcac aactgcgcgc tcaccacgaa
     1321 cggtgccgcc gattgctggg gtctgcccga cgacggccaa gcgaacgacc ggcccggtcc
     1381 gttcacgcag ctctcggcgg gtgaccacca caactgcggc ctgcgcctcg acggttccgt
     1441 ccgctgctgg ggacacaacg acttcggcca ggccgcggat cggctggggc cgtacgtcga
     1501 ggtgtcggcg ggcagctacc acacgtgtgc gaccaccgcg gcgggcgaca ccgattgttg
     1561 gggcgacaac cgcgacggac agctgggtcg gcgcctcacg atctcggcgc cgggcgcggt
     1621 aacggcggga tcgcgcgcgc gcatcacagg gcgcctccag tcgatcgatc cggcctgcat
     1681 cgcttcgcag accgtcgcgc ttcgagtgct ccgcccggcc ggcgttccca gccgcacgac
     1741 gatgacctcg acggcgggtc gctacacgtt caacgtgaag atcacgcgcg aaaccgtcgt
     1801 ccgagtcgag ttcgcacccg ccgggtcgtg cgaacgggtc cgttcgacgc ggcgcacgat
     1861 cgtagccacc tgacccgatc tcgttccctg agtgcaacgc cgctgttcgc taaccgatcg
     1921 gttccttcgg ctcccacagc tggatcgcgt ttccctcggg gtcgttcagc tgggcgaacc
     1981 tcccgttcgg atagtccgtc tcgtcgacct caacgtcgac gcccgccgat ccaagctgcg
     2041 caacgatcgc gtcgagatca cgcactcgga agttgacgcc ccagccgcct cgcccgatga
     2101 ccgggctctc agcggcatct ggtccgaagg gcgcgaagac ggtcgggccc tcgcgctggg
     2161 tccacaccgt tgccccgtag gaggtgggaa ccgggtcgat ccccaagtgc tgctcgtacc
     2221 acgcggccag cccgtcaggg tccgccgccc tgaagaagaa gcccccgatg ccgtcgaccc
     2281 gctccatccg gaggccatcc tcgcacgttc ccccgagcgg agccgaccgc gctggatcgt
     2341 ggttccgggg ggaggaatcg aacctccatt ccaggctcca aaggcccgtg tcttgccgtt
     2401 agacgacccc ggagcggggc gacctgcgcg ccgctcgccc ggggattatc ccgcggcggc
     2461 ggggtcggtg gcccgtggcc tggtcgaaac gccacacgac ttcgccgtca accagctcat
     2521 cggtgggctg catccccaac cgatcggcga ccgatccgga cgccgtctgg tccggatgga
     2581 tcgacgcggc ggtgatcacg cgggcgggcc gctcacgacg atggcgccgc ccaccgcttc
     2641 ggccagccgg tcggcatgac ccatgtgcga ggcgagcgcc gccacggtgg gaccgctgcc
     2701 gctcatgatc gcgccgaggg cgccggcctc catgaaggca tcgatcgcct cgccgacctc
     2761 cggatgacgg gccaccacgg gggcctgcag gtcgttgaac agggcgctgc cgagcagcgc
     2821 gagatcacca gtctcgagcg ccgcgatcag caccccgggg tcgggaccgg tcgtgggatc
     2881 gtggtcccac cacccgtagg cgtcggcggc cgtcacggcg acgtcgaacg gcttgagcac
     2941 ccaccaggtg gtcaccgtgt ggacgggggt gagcacctca ccgcgcccgg tcaccatcac
     3001 cgggccgccg gccagcatgg cggggacatc ggatccgagt tcggtagcga tggacgtcag
     3061 gctggctggg tcgtcatggc cgccgtccac ttcggcgagg agatcggcca ggccgaggag
     3121 agtggcggcc gcgtcggcgc tgcccccacc cagtccggcc gccaccggga tgcgtttgtg
     3181 gatgtcgacc cggaagccgc cggggatgcc ggagcgcttc gacagttccc gcacggccgc
     3241 gatcaccagg ttgtcctcgc ccgtcggtac ccgtccggtg agttcgggga cgccctcgac
     3301 cgtcaccgac gggtccgacc ccccgtcggg cgacaccgtg acgaggtcgt gcagactcaa
     3361 tggcagtacc acgctctcca gctcgtggaa cccgtcctcg cgccggccta ggacacgcag
     3421 gaacacgttg atcttcgcgt gcgcccggcg ggtgatcggc tcggtgctca cgccgggacc
     3481 gccgaggcga tggcggcgaa cgtctccagg tcgagttctt cggggcgggc cgaggccgac
     3541 acgccggtcg cgatcaggat cgcgtcggcc tccgtggccg gcaggtcgag gcggcgcaag
     3601 gcgttgcgca tcgtcttgcg gcgctcggcg aacgcgccgt cgaccactcg ccacagggaa
     3661 gcggcatcca cgtcgaccgc cggggaagac aatcggtcga gccgcaccaa caccgagtcc
     3721 accgacggct tcggccagaa caccgtggac ggcacccggc gggtgatcgt cgcgcgggcc
     3781 cggtaagcga ctttgatgct gaccgcgccg tagtgctgat cgccggacgt cgcagcgagc
     3841 cgttcgccca cctctcgctg caccatcacg acgaggcgcc ggaccgcggg cgcggacgcc
     3901 agcacctcca tcacgaccgg cacggcgacg ttgtagggca ggttggcgca cagcgtccac
     3961 gcgccggcat cgcccagcgt ggaggcccag tcgagcttgg tggcgtcggc gtgcaggatc
     4021 cgaacgttcc cggctgagcc caccacctcg gtcagcgccg ggatcagcgc ccggtcgaac
     4081 tcgatggcga gcacctgggc gccggtggcg gccaaggcca tcgtgagcga gccgagaccc
     4141 gcgcccacct ccaccacccg atcgcccggt ccgacgcccg cctccgcagc gatcgctctg
     4201 gccaggttcg ggtcgatgag gaagttctgg cccagggact tggtgggacg gatgccgtga
     4261 cggcgggcga ccgctcggag ggccccggcc cccaaggaac cggggagcga ggtcaggccc
     4321 tcgccgggtt caccagctga gttggacgtc gatgacgccg gtgccgaggg ggcggaggac
     4381 ggcgaaggcc tccggcgaga ggtcgatgat cttgcccggg gcgaacggtc ctcggtcatc
     4441 gatgaccacg gtaacggagc gaccggtctc gacgtcggtc accgtcacca tcgtgccgaa
     4501 cggcaggctg ggatgggcgg cggtcaggcc ggaccacggc gggtcgtacc aagacgccaa
     4561 tccggtctgg gacgcgccgt cggtgccggg cacctccgtg gtgccgccgt acatcgactc
     4621 gggcgccgac agccgctcct cgtcgaccgg atcgcgctcg atccagcggg cgatcagcgc
     4681 ccgttcgacc tcctggccgt tgacgacggt gacgcggtac gtgccgaccg cgcgcccgtc
     4741 ctggcccttc tggaggacct tcacgttccc cggcaccatg ctcggggtga accgggtgtg
     4801 gacgtcgaac gggatcgttt cgtcttcgag ctcggtcgtg acctgcacct ggtcgtaggt
     4861 gagggtcgcg ccggattgaa ggggggtgct gggtgatggc gtcacgcggt catcagcgtc
     4921 ggcgttgatc cccatggccg agaggagttc cccggtcgag cccgcgttgg tcaggacgtc
     4981 gcgcaccttc cccagcacga cggcccgcac ggacacgacc ggggacgacc cgacgctcga
     5041 tgcggagaag gaactctcga cgagctcgtt cgcgagtccc gcgatgttcg agttcgtccc
     5101 agccatcacc cagaccccca catcggtggg actctgatag acggtggtcg catccgaagc
     5161 gatcagatcg cgaacgcccg gcgcggccgg cgccggactc accaccaccg tcatgccgtc
     5221 ggccagttcg gtggccggcg gcggatccac ctgcacgctc gacgtgagcg agatgccctc
     5281 gccggcgagc agttcgtgga cgttggatga ggtcgtctgg acggcttgcg acttcccgtc
     5341 gacgaccaag gtcacgtgct tgcggagcgc gaagtggacg acgcccgaca gcaggagcaa
     5401 gccgaccacg acggtgatcg cttgaaccgc tcgatgggct cgcaacctgc gaacccgggt
     5461 ggggcggacc gccaaccttc ctccaactgt ctgaggaagg cttcaggggg aagcggctca
     5521 gccgtaccga agccttcatg cgcgtgagag ggcaccctaa caaaccctca accctcactg
     5581 caatcttgag accgaacgta tgttcgggcg ttatcggagc cccgggaagg cgcttcgcgc
     5641 gttctccgcg gtggccgtga cgatcttttc cacatcgtca tcgcgcacac gtgcgatctc
     5701 ggcgatcgtc aacatgacgt tcgacggttc gttgttcgag ccgcgcagct tctgcggcga
     5761 caggaacggt gagtcggtct cgacgacgat gcgatcgagg tcgaccgcga cggcggcctg
     5821 tcgaaggtgt tcgttcttcg ggtacgtcac gttgccggcg aagctgatcc agtagccgcg
     5881 cgcggcgcac tcacgtgcga tcgcggcgtc tccgctgaag cagtggatca ccacgcgctc
     5941 ggcggaaccc tcatcgagga ctcgaaggac gtcgggccat gcgtcgcgga cgtggaccac
     6001 catcggcttg cccgtctcgt tcgacaaggc gatgtgggtc tgcagcaccc ggatctgatc
     6061 ctcggcgggc gagtgcatcc ggaaatagtc caggccgcac tcccccacac cgatcacgcg
     6121 tgcgtcgtgg agcagttcct cgatccgtgc cgaggcggcc tgatcgaagt cggaggcgtc
     6181 gtgtggatgc atgcccgccg tggcgaacac accctcgagg gaatcggcca gttcggcgga
     6241 acgggcgctc gtggcggggt cgacaccgac gcagatcagc cggtcgacgc ccgcggcctt
     6301 cgcggtctcg accacttgtg caggttcgtg gtcgagcagg aacaggtggc agtgggtgtc
     6361 caccgccccg gtgtcagtcc ctggttgaag gttctggtcg ctcacgagcg gcgagcttac
     6421 aagggtctcc acatacggtc gatgcgggga gcttcacgtg aaacacgatt ccgggaagcc
     6481 tcacccccga ctgaaggcgt ggtggataac tcgggcgcgg ccggatcggc ctgcgaacgc
     6541 ctcagtcggc gtccaggcgg gggaagagcg actcgccttt ggtggtggag gtacccacct
     6601 cgaggcctcc ccactccacc gacgcgggca cgcgctgacc ctcgagtgag gccgtctggc
     6661 cgagctggct ccacaggcgg ccggcggccg ccggcatgat cgggaagatc aggacggcga
     6721 ggatccggag ggtctcggcg ctggcataca ggacgctggc gagctcctcg cgcctgccct
     6781 cgtccttcgc gagcttccag ggctcggtct ccaccaggta gtggttggca cgatcgacga
     6841 tgtcccagac cgcgatcagc gccggctgca gatggacggc cagcatgtgg tcgtcgtagc
     6901 gggcaacggc gtcggccacc acgctcggca ggtccgactc ggcaccttcg accaccggcg
     6961 cggggaccgt gccgtcgaag taggaaccca gcatcgcgag cacccggctg gcgaggttgc
     7021 ccagcccgtt cgccaggtcg gcgttgtggc gttcgaccat ggactcgaaa ctgaagttgc
     7081 cgtcctcgcc gaaccgcatc tcgcgcatga agtagtaccg gtaggaatcg atgccgaact
     7141 ggtccagcag ctcgaacgga tggatcccgg tgaggttggt cttcgacatc ttcttgccac
     7201 ccaccgtgag gaacccgtgg gcccatacct gcgtgggcgg ctcgacgccg ccggccatca
     7261 gcatggccgg ccagtagatc gcgtggaacc ggaggatgtc cttgccgatc aggtggatgt
     7321 tggcgggcca cacccgggcg aaccgatcgg ggtcggaggc gtagcccgcg gcggcgatgt
     7381 agttggtgag cgcgtcgaac cacacgtagc agatgtgctt cgggtcccag gggatcggca
     7441 caccccagct gaacgacgtg cggctgatcg agaaatcctg caggccgcca cggatcaggc
     7501 tgagcacttc gttgcggcgc gtctcgggct cgaccgccga gggatgctgc tcgtaatgct
     7561 ccagcagccg atccgcgtag gcggagagcc ggaagaagta gttctcctcc accagatgct
     7621 ccacgggggt ttcgtggatc gggcacaggc cgccctcgag gagttcgtcc tcgctgtaat
     7681 aggcctcgca cgaaacgcag tagagcccct cgtaggaacc gaggtagatg tcatcccggc
     7741 cgttgtcgtg cacggcgttc agcagggcgg caacggcgtg ctcgtgacgt ggctgggtgg
     7801 tgcggaggta gtcgtcgtag ccgatgtcga gccgcgccca cacctcgcgc cacttgggct
     7861 ccatgtcgtc gacccactgc tgcgggctga cccccgcgcc ttcggccgcc cgttggatct
     7921 tcagcccgtg ctcatccgtg ccggtgaggt ggaagacctc ctcgccgcgg agccggtggt
     7981 agcgcgcgat gaagtcgttg gccaccgcgt tgtaggcatg cccgatgtgc ggcacgtcgt
     8041 tcgggtagta gatcggcgtg gtgatgtaga agacgtccct gcccatggtc gggccatgat
     8101 acgggagtgc gccactagtc tgaggcagac tagtgtgcac cgctcgtgga tctactgctt
     8161 gccgaagagg gtgccgaaca ccgagcgcag ggtcttgcgc ccgtcggccg tggtgagcat
     8221 gtccttggcg aagtcgaccg cgacgccctt cacctggtcg cccaccccgt cgctctcctt
     8281 gggcttggaa ccggacgtgg ccttgggctt cgactcggcc ttcggccggg gcttcggtgc
     8341 cggcgtcggt gacgccgggg gcgaaccggg aggtggaggc ggcagcggca cggacccggc
     8401 cggtgccgca gggacgccgg cagcttcctt cgccgttccc agacgcgcac tgatcatctc
     8461 gtgggcgctc tcgcgatcga cggcctgcgc gtacttcgct cccagcgcgc cgtcggccag
     8521 caatcgctgg aaggacgttt cgtccaccgc atccatcagg gaatcgggcg gcacgagccg
     8581 ggtggcggcc agcggggtgg gcaccccttt gggggtcagc agggtgacca gcgcctcgcc
     8641 gatcccgagc gaggtgagcg tggccccgac gtcgtagtgc tcggtcttcg ggaaggtctt
     8701 caccgtcttg ttcagcgcgg tctcgtcgtc gggcgtgaac gagcgcaggg cgtgctggac
     8761 gcgattgccc aactgggaga gcacggagct cggcacgtcc gtcggcgact gcgtcacgaa
     8821 gtacacgccc acgcccttcg atcggatcag gcgggcggtc aactcgatct ggtcgagcag
     8881 cgcctccgag gcatcgtcga acagcaggtg tgcctcgtcg aagaagaacg ccagcttggg
     8941 cttgggcagg tcgccttctt ccgggagctc ggcgtagacc tgggccagca tccacaacat
     9001 gaaggtgctg aacaggcggg ggcggtccat cacgtcgctg atctccagca ggcttaccac
     9061 gcccttgccg tcgggcgtga tgcgcagcag atccaccacg tcgaactggg gctcgccgaa
     9121 gaagtcgccg gctccggctt cctccagctc gacgagcgag cgcagcagca cgcccacgct
     9181 cgccttcgaa acgcccccgt actcctcgag caccggcttg ccgtcgtccg aggacaggaa
     9241 cttcaaggtc gtccgcaggt cggccagatc gagcaacggg tagttctcgt cgtcgcagta
     9301 cttgaagacc agcgagagca ccgaggtctg cgtcttgttg agatcgagca ccttggccag
     9361 cagcagcggc ccgaacgagt ggatcgtggc gcggacctgc gccccgaggt cgccgctcag
     9421 cgacagcagc tcgacgggat gggccttggg ttcgaacgtc cagcccagct gcgcgcaccg
     9481 ctcggccacc ggagctgcgg cggcatcgcc cggctgggag atcccggtga ggtcgccttt
     9541 cacgtcggcg atgaacaccg ggacgccggc gtccgaaagc tggcccgcca ggatctgcag
     9601 cgtcttggtc ttgccggtgc cggtggcgcc ggcgacgagc ccgtggcggt tcaccatgga
     9661 cagcggcagc tggaccctgc tgcccaggtc gacctcgccg tcgagcatcg gcccgcccag
     9721 caccacacag ggctcatcga aggcgtagcc ggccgccatc tcccgggcga aacgctcgtc
     9781 catgcgcgga gcctagtgca ggcccgacga gcgcgggact cagccgcgct tgagcagttg
     9841 gcggtacacg tcgttggccg agacgtggtg tcgctcggcg acggcgtggg cggcgtcgcg
     9901 tttgcgcatg ccctcgtcga cgagctcctg cgcctcgtcg gcacacgtct cgagatcggg
     9961 ggtctcgggc gtgtgctcgc catcgatcac gacgacgacc tcgcccttga gctcggcgtc
    10021 gccgatgtgc gacaggacct ccgagatccg cccgcgcatg acctcctcgt ggagcttggt
    10081 gagctcacgc gcgatcgcga tgcgccggtc gcccaacgcc accaacgcgt cgcgcagcag
    10141 gatctgcacc cgccgcggag actcgaagac gacgagcgtc ctcgggtcgt ggcggagggc
    10201 gtcgagccgg cgcatgcgct cccccggctt gcgcggcagg aacccctcga acgtgaaccg
    10261 gtcggtgggc aggccggaga gcaccagcgc tgctagcgcg gccgatggcc ccgggacgac
    10321 cttcacgtcg atcccggctt cgatcgcgcc gtgcaccagg cggtagccgg gatcggagac
    10381 cgagggcatg ccgccgtcgg tgacgagcgc caccgagctc cccgaccgga ggcgttcgat
    10441 cagctcgtcg gtgcgctcgc gttcgttgcc ttcgaagaac gagaccatcg gcttgcggag
    10501 agacaggccg tggagcaggc ggccggtccg gcgcgtgtcc tcacatgcga ccaggtccac
    10561 gtcggccaga acctcgcgag cccggggcgc gaggtcgccc aggttgccga tcggcgtgcc
    10621 cacgagccac agcgttccct ccacggttcc agggtgacac gatgaggtcg ctgcgaccat
    10681 gcgtcgaagg acctggatgg aaggacaccg gtggccgacg ggcacaagga ctactccacg
    10741 acccccctgt ggaggaagct cgggatccgt gaaggctcca gggtccgtct gctcaacgca
    10801 cccgacgggt tcgaggacgc gctcgtcgcg atcgcgccgc tgccgaacgc ggtggacttc
    10861 ctccgccgct cagccaaggg catcgatgtc gccgtcctgt tcaccaccga acgacaggtg
    10921 ctcgaacgcc ggttcggcgc actggcgacc acgctggagc ccgacggccg gctctgggtc
    10981 gcctggccga agaaggcctc gagggtggcg accgacctga cgttcgagat cgtgcagtcc
    11041 cacggactcg ccggtggcat ggtcgacaac aagtccgccg cgatcgacga ggtgttccag
    11101 ggcttgcagt tcgtctaccg ggtgcaggac cgacccggtc gatgagtccg cggtcgggat
    11161 gttcggaccc ggcccggata ccctgacgcg atgtccgccc tcctgcagcg cctgaaccgg
    11221 ccgatcgtgg cgatcctcgc cgtggcggcc ttggccggca tcgcccgcgg gtgggccttg
    11281 gcgaacccgc ccgatctggt cttcgacgag gtctactacg cgaaggcggg ctgcatcttc
    11341 gtcggcggtt cggaccagac gtgccgcatc gacgaggacg ccgagcacta ctgggtcgag
    11401 cacaagtggg acgtgggctc gtgggtccac cccccgctgg gcaagtggat gaccggcatg
    11461 ggcatcaagg cgttcgggat gggcgccttc gggtggcgct tcccgtcggc cgtggccggc
    11521 acgctgatcg cggtgatggt ggcggtgatc gcccagttgc tgttcggccg gccgctgtgg
    11581 acgttcgtcg ccggtgggct gatcgccctc gacggtctca acgtggtgat gtcgcgcgtc
    11641 gcgatgttgg acatccatct cgcgttctgg gcggtgctgg gcttcctgtg tctggtgctg
    11701 gaccggcgat ggatcgaccg tcgcaccgaa cgtgcgctcg ccgatgaacc cgtgcagaca
    11761 gtgaccgtgg tcggtgagga cggagccacg gtcctggtcg aggggcgcgc gggggcggcc
    11821 gggcgcgggc ggacgcgggt gccctcaccg ttgtggcggc cgtggcggtt cgcctcgggc
    11881 gcggcgatgg gcgccgcctg tgcggtgaag tggtcggggc tcacggcgct cgcggccgcc
    11941 gtgctggtca ccgtcgtctg ggagaccacc cggcgacatg tcccaggggt gtcccgctgg
    12001 cgggcgttct ggcggaccgt gggcatggag ctgctcggcc tgatcctggc cttcctggtg
    12061 gtgccggcga tcgtgtacat ggcgacctgg ctgccgtggt tcaaccactt cgggtggagc
    12121 ctgtccgcgt ggtgggagaa ccagaaggag atgtggcgct accacgccaa cctcaccgag
    12181 ttcgcctacg actccaagac cgactcgttc acgcccaccc acggctccta ttcgaaggca
    12241 tggtcgtgga tcttgatgaa acgtcccgtc aacttcttcg tggagaacac cacgggccac
    12301 gtccggcaga tcctcacgat cggcaacctc gccctgttct ggggcacggt gttcgcgatc
    12361 ccgtacgcgg tgtggtcgtg ggcgaggacc cgcgactggc gtgcgggctt cgtggtgatg
    12421 ccgttcctgg tgatgtggct gccctggttc gtcgtgagcc gtccgcagtt cttcttctac
    12481 gtgttgccga tgacgccgtt catggcgctg gcggccgtct acaccctgca ggccctctcc
    12541 gacgcgaggc tggtgctcca cgacgaggcc ggagaggtgg cgatcgatcc ggagaccggc
    12601 cgtcccgcga tctccacgcg ccatccgttc cgcccggtgg tgatgggcta cctcgcgatc
    12661 ttcgtggtgc tgttcgtctg gttctggccg ttgctggtgg gcgagcgcct caccacgtcg
    12721 ttccgtgcgc tccacatctg gatgcgcagc tggaactgac caacggtcag cgtccgcgga
    12781 gccgggcgct gcgcgggcgg cggagccgcc ggtaccctgc cccgcatgac ccgccgcgag
    12841 cacgtgtgga tccccatgga cgacggcgtc cgtttggccg ccaccctcta tctccccgac
    12901 ccagcggatg gtggcgacgg cccgtggccc gcgttgctcg aggcactgcc ctaccgcaag
    12961 gacgacgtga ccgcgtcgta ccggccggag taccaacggc tcgccgaggc cggctacgtg
    13021 atgtgccgcg tcgatgtccg cggcacgggc accagcgaag gtatcgccga ggacgagtac
    13081 cccgcgatcg aacgcaccga cctcgtgacc gtgatcgact ggctcgccac ccgcgactgg
    13141 tccaccggtt cggtggggat gtacggcacg tcgtactcgg ggttcaactc gatccagctc
    13201 gcgatggaac ggccgcccgc gttgaaggcg atcatcccga tcttcgccac ggacgaccgg
    13261 ttcggcgatg acgtgcacta cttcggcggc gcgctgaagg cgctcgacct cgtggactac
    13321 cccacctaca tggtggcgat gaacgcgct
//
LOCUS       DATDBZ010000187         2837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_676451, whole genome shotgun sequence.
ACCESSION   DATDBZ010000187 DATDBZ010000000
VERSION     DATDBZ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676451"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VIX62_04600"
     CDS             complement(<1..775)
                     /locus_tag="VIX62_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5859543.1"
                     /translation="MSWDFETDPEFQAELDWVDDFVRNEVEPVDQVIHHAWDMKDPVR
                     QALIPPLQQQVRDRGLWACHLGPNLGGPGYGQVKLALLNEILGRSHSAPIVFGCQAPD
                     SGNAEILAHYGTDELKKRYLQPLLDQEVVSAYSMTEPRGGSDPTGFVTRAERDGEEWV
                     INGEKWFSSHANFAEFLIVLAVTDPNEERHRRMSMFVVDKATPGVEIIRNVGIYGHPE
                     EEATHSYVRYTDVRIPADHLLGGIGEGFLVAQTRLGGGRI"
     gene            complement(871..1626)
                     /locus_tag="VIX62_04605"
     CDS             complement(871..1626)
                     /locus_tag="VIX62_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY5859544.1"
                     /translation="MKIQAGTTVLRPLKTSETVARDIVHSVVTGGLGQGDSLPSEARM
                     LEQYGVSRESLREGLRLLEAQGLITLRRGPGGGPVVGTVDPANLGRASTLYYHLVGAT
                     YGELFEAWAVAEGYLAERAARNEDRALVRATLEPYLAAEDDDAFGGDLEAFVTHHTAF
                     HTDVGELAGNRVMQLSLMAIGQTVTHHIAYSADPRDVADILLHDHRVIAAAIIAGKPI
                     KSRDEMRIHLEGVAAFYNRLSGGAVLDQLIEWR"
     gene            complement(1724..>2837)
                     /locus_tag="VIX62_04610"
     CDS             complement(1724..>2837)
                     /locus_tag="VIX62_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5859545.1"
                     /translation="DEERALLDRAMAWQREKFDAGYGALTWPAEFGGAGLGDEFAAAF
                     DELESAFVHPPHHETFSVTLHLVAPTLAQFGQTDLRSQLLPRLLRTEALSCQLFSEPG
                     AGSDLGALATRAERDGDSWAVNGQKTWSSGARFAEWGELVARSDHDVPKHEGMTAFML
                     EMDSPGVTIRPIRQMSGGSSFNEVFFSDVRVPDAHRLGDVGEGWRVALTTLGFERRSS
                     GSGRGMSTVGGSFQQLLALARWLDRDGDPLVRQRLADVYARERLRELVGARTLAAVAD
                     GGVPGPYASIGKLMWTRSMTRIGAVAAELLGPRMTADTGEWGTYAWTKHLLGAPGYQI
                     AGGSDEIQHDIIGNRVLGLPPEPRPDKGVAFRDVPR"
BASE COUNT          451 a          950 c          959 g          477 t
ORIGIN      
        1 ggatacgtcc gccgccgagg cgggtctggg ccacgaggaa cccttcgccg atcccaccga
       61 gcaggtggtc ggccggaatc cgcacatcgg tgtagcgcac gtagctgtgg gtggcttctt
      121 cctcgggatg gccgtagatg ccgacgttgc ggatgatctc gacgcccggt gttgccttgt
      181 cgacgacgaa catcgacatc cgccggtgcc gctcctcgtt gggatcggtc accgccaaca
      241 cgatcaggaa ctcggcgaag ttggcatggg acgagaacca cttctcgccg ttgatcaccc
      301 actcctcgcc gtcgcgctcg gcgcgtgtga cgaacccggt cgggtcggac ccaccgcgcg
      361 gctcggtcat cgagtaggcc gacaccactt cctggtccag gaggggttgt agatagcgct
      421 tcttgagctc gtcggtgccg tagtgggcca ggatctcggc gttacccgag tcgggcgcct
      481 gacacccgaa cacgatcgga gcgctgtggg agcgcccgag tatctcgttg agcaaggcca
      541 gtttgacctg gccgtacccg ggcccgccga ggttcgggcc gaggtggcag gcccacaggc
      601 cgcgatcgcg gacctgctgc tggagcggtg ggatgagcgc ctgacgcacc gggtccttca
      661 tgtcccacgc gtggtggatg acctgatcga ccggttcgac ctcgttgcgc acgaagtcgt
      721 cgacccaatc gagctcggcc tggaactcgg ggtcggtctc aaaatcccaa ctcacgacgg
      781 cattcccctc ttgacaagtg cgacggcctc catgtgcgcc gaacgcccac cccttgtcaa
      841 gagttcatca tgataaatta tcgaattcgc tcaacgccac tcgatgagct gatcgaggac
      901 ggcgccaccc gacaggcggt tgtagaaggc ggccacgcct tccaggtgga tgcgcatctc
      961 gtcgcgcgac ttgatcggct ttccggcgat gatcgccgcc gcgatgacac ggtgatcgtg
     1021 gaggaggatg tcggcgacat cacgcggatc ggcgctgtag gcgatgtggt gcgtgacggt
     1081 ctgcccgatc gccatcagtg acaactgcat cacccggttg ccggccagct cgccgacgtc
     1141 ggtgtggaac gccgtgtggt gggtgacgaa cgcctcgaga tcgccgccga aggcgtcatc
     1201 gtcctcggcg gcgaggtagg gctcgagggt ggcgcgcacg agcgcacgat cctcgttgcg
     1261 ggcagcgcgc tcggcgagat acccctcggc caccgcccag gcctcgaaca gctctccgta
     1321 ggtggcgccc accaggtggt agtacagcgt ggacgcccga ccgaggttgg cggggtcgac
     1381 cgtgccgacc acgggacccc cgccgggccc gcggcggagc gtgatcaacc cttgggcttc
     1441 gagcaggcgg agcccttcgc ggagcgactc tcggctgact ccgtactgct cgagcatgcg
     1501 agcttccgac ggaaggctgt cgccctgtcc gagaccaccg gtgacgacgg aatggacgat
     1561 gtcgcgggca acagtttcgg acgtcttcaa tgggcgcaac acggtggtgc cagcttggat
     1621 cttcattcgg gtctgttgct tgggcacagc ggcaggctag tgggcgtcgt cgtttgcgaa
     1681 ccgggtccgc ctgcgcgctc gacggcacct cggatcgagc gagtcatcgc ggcacatctc
     1741 gaaacgccac gcccttgtcg ggacgcggct caggggggag cccgaggact cggttgccga
     1801 tgatgtcgtg ctggatctcg tcgctgccgc ccgcgatctg gtagccgggc gccccgagca
     1861 ggtgcttggt ccacgcgtac gtgccccact cgccggtgtc ggcggtcatc cgcggtccga
     1921 gcagttcggc cgcgaccgct ccgatccggg tcatcgatct cgtccacatc agcttgccga
     1981 tcgacgcgta cggtcccggg acaccgccgt cggcgacggc cgccagtgtc cgcgcgccga
     2041 cgagctcgcg gagccgctcg cgcgcataca cgtcggcgag acgctgccgc acgagcgggt
     2101 cgccgtctcg atcgagccat cgggccaggg cgaggagctg ctgaaaggat cccccgacgg
     2161 tgctcatgcc gcgaccggag ccgctcgacc gacgttcgaa tccgagcgtg gtgagcgcca
     2221 ccctccagcc ctcgccgacg tcaccgaggc gatgagcatc gggaacacgc acgtcggaga
     2281 agaacacctc gttgaacgac gagccgcccg acatctgccg gatcggccgg atcgtcacgc
     2341 cgggactgtc catctcgagc atgaacgcgg tcatcccctc gtgcttcggg acgtcgtgat
     2401 cgctgcgggc cacgagctcc ccccactcgg cgaaccgtgc acccgagctc cacgtcttct
     2461 gcccattgac cgcccaggag tcaccgtcgc gctcggcgcg agttgccagc gcgccgagat
     2521 cggagccggc ccccggttcg gagaacaact ggcagctcag tgcttctgtc cgcagcaggc
     2581 gcggcagcag ctgggagcgc aggtcggttt ggccgaattg ggcgagcgtc ggcgcgacca
     2641 gatggagcgt cacgctgaac gtctcgtggt ggggcgggtg cacaaaggcg gattcgagct
     2701 cgtcgaatgc ggccgcgaac tcgtcgccca atccggcgcc gccgaattcg gccggccacg
     2761 tcagggcgcc gtatccggcg tcgaacttct cccgttgcca cgccattgcg cgatcgagca
     2821 gtgcgcgttc ctcgtcg
//
LOCUS       DATDBZ010000188         3078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_38944,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000188 DATDBZ010000000
VERSION     DATDBZ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38944"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VIX62_04615"
     CDS             complement(<1..873)
                     /locus_tag="VIX62_04615"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY5859546.1"
                     /translation="MLTTGSTPLRARVAGFVALTKPRIIELLLVTTVPTMVVAQRGLP
                     SIWLMVATVVGGTLAAGGANAINMYVDRDIDAVMERTKDRPLPTGVVKPREALVFAIA
                     IEIAAFVWLWAFVNLLSAVLAVSACLFYVFVYTLWLKRTSERNIVIGGAAGAVPVLVG
                     WSAVTNSLDWAPVVLFAVIFYWTPPHFWALAVKYKDDYAAVDVPMLPSVVSLRTTAIR
                     IIYYTLVLWALTILFWPVAEMGWIYGATAVVLGGVFTWFAVALLREQTTRSAMRLFGW
                     SITYVTLLFGAMALD"
     gene            985..1947
                     /locus_tag="VIX62_04620"
     CDS             985..1947
                     /locus_tag="VIX62_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY5859547.1"
                     /translation="MARPSSPVERSPSRTVSPAGFRRITLAVLVTQVIIVLTGAAVRL
                     TGSGLGCSDWPNCEEDRFVPATDFNAVIEFVNRLMSFPVLVAVVLAVWAAGRRVPFRS
                     DLRTLSWAILGGVVVQVLLGAVVVRLELLPSTVIVHFLISMGLIALAVVLHHRAGSDL
                     PRHATPGGWRPTASTSLMIPLALMSAAVLAAGTVVTAAGPHGGDENADRLPVFLPTAT
                     RVHSLAAWAFLALLVVVLVLLRRERAPQVVHRCGTIVLVTCLIQGGIGYLQYWNGVPA
                     GLVFLHVAGAMAVWGTTLWFVLEHRRLASTTTTTAAATATAGSS"
     gene            1944..2234
                     /gene="clpS"
                     /locus_tag="VIX62_04625"
     CDS             1944..2234
                     /gene="clpS"
                     /locus_tag="VIX62_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY5859548.1"
                     /translation="MSGPSPVETESPIGDVDEALEGPWVVIVWNDPINLMSYVTFVLQ
                     KLFGYSIEKATELMLDVHQKGRAVVSNGSREKAELDVFRLHEHGLWATMQQD"
     gene            2245..2733
                     /locus_tag="VIX62_04630"
     CDS             2245..2733
                     /locus_tag="VIX62_04630"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEY5859549.1"
                     /translation="MSRDGFGFFDRRWFRPADAGGFDVAVPSGVRAILGDMVGSLDEL
                     LSSDSENIRRVFPTAYPDDAEREAGYQAFVRGELIERHSAAAEVLASTIDADHLTEEQ
                     LTSWMTTANTLRLVFGTALDVSEDDDPAIDPDDEDAPARVAYQVLTELVDDIVQALST
                     TL"
     gene            complement(2776..>3078)
                     /locus_tag="VIX62_04635"
     CDS             complement(2776..>3078)
                     /locus_tag="VIX62_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859550.1"
                     /translation="DATIDRDGDLVTLRSCDPGTDDLADPVTDPLDAIALPATRSYVM
                     LGGTQEGLPVEASACIGDAVVEQVPLEVLADPEPSDEELDHLFEVMGGAGSKCAAA"
BASE COUNT          502 a         1080 c         1014 g          482 t
ORIGIN      
        1 gtcgagggcc atggcaccga acaggagcgt cacataggtg atggaccacc cgaagagccg
       61 catggccgag cgggtcgtct gctctcgaag gagcgcaacc gcgaaccacg tgaacacccc
      121 gccgagcacg accgcggtcg ccccgtagat ccagcccatc tcggcgacgg gccagaacag
      181 gatcgtgagc gcccacagca cgagtgtgta gtagatgatg cggatcgcgg tggtccgaag
      241 cgacaccacc gacggcagca tcggcacatc gacggcggcg tagtcgtcct tgtacttcac
      301 cgccagcgcc cagaagtgcg gcggcgtcca gtagaagatc actgcgaaga gcacgaccgg
      361 cgcccaatcg agcgagttgg tgacagccga ccagccgacc agcaccggaa cggccccagc
      421 agctccaccg atgacgatgt tgcgttcaga ggtccgcttg agccacagcg tgtacacgaa
      481 cacgtagaag aggcaggcgg acacggcgag gaccgcgctc agcaggttga cgaacgccca
      541 cagccacacg aacgccgcga tctcgatggc gatcgcgaac acgagcgctt cccgcggctt
      601 gacgactccg gtggggaggg gtcgatcctt cgtccgttcc atgaccgcgt cgatgtcgcg
      661 gtccacgtac atgttgatgg cgttcgcgcc gccggccgcg agggtgccac cgacgacagt
      721 ggcgaccatc agccagatcg aagggagacc cctctgtgcc accaccatcg tggggacggt
      781 ggtgacgagc aggagctcga tgatcctcgg cttggtgagc gccacgaagc ccgcgacacg
      841 tgcgcggagg ggtgtggacc cggtcgtgag cactcggcga gcgtagggcc gcatgggtcc
      901 tcattgccaa agcgtcagcg acgtccgacc cttcggctca gccccgcttc gacccgcagc
      961 gacgccggtc ctaccatcgc cgtcgtggcg cgcccctcct cccccgtcga gcgctcgccg
     1021 tctcgcaccg tctcgcccgc cggcttccgc cgcatcaccc tggccgtcct cgtgacacag
     1081 gtgatcatcg tgctcacagg tgccgccgtg cgactcaccg gttcggggct cggctgctcc
     1141 gattggccga actgcgaaga ggaccggttc gtgccggcca ccgatttcaa cgcggtcatc
     1201 gagttcgtga acaggctgat gtcgttcccg gtcctggtcg ctgtcgtcct cgccgtctgg
     1261 gccgcggggc ggagggtgcc gttccgttcg gatctgcgga ccctgtcctg ggcgatcctc
     1321 ggcggggtgg tggtccaggt cctgctcgga gccgtcgtcg tgcgcctgga gctgctgccc
     1381 tcgaccgtca tcgtccactt cctcatctcc atgggcctca tcgccctcgc ggtcgtgctc
     1441 caccaccgcg ccgggagcga tctcccccgc cacgccacac ccggtgggtg gcggcccaca
     1501 gcctccacgt cgctgatgat cccgctcgcg ttgatgtcgg cagcagtcct cgccgccgga
     1561 accgtcgtca ccgcggcagg accacacgga ggcgacgaga acgccgaccg cctccccgtg
     1621 ttcctcccca ccgccacgcg tgtccacagc ctggcggcat gggcgttcct cgcgctgctc
     1681 gtcgtggtcc tggtgctgtt gcgccgcgag cgcgctcccc aggtggtgca ccgctgcggc
     1741 acgatcgtcc tggtgacgtg cctgatccag ggcgggatcg gctacctcca gtactggaac
     1801 ggcgtgccag ccgggctcgt gttcctccac gtggccggcg cgatggccgt ctggggcacg
     1861 acgctgtggt tcgtgctcga gcatcggcgg cttgcgtcca ccacgacgac caccgcggca
     1921 gccaccgcga ccgcaggttc atcatgagcg gcccgtcgcc ggtcgagacc gaatcgccca
     1981 tcggtgacgt cgacgaagcg ctcgagggac cgtgggtggt catcgtctgg aacgacccga
     2041 tcaacctgat gagctatgtg accttcgtgc tccagaagct gttcgggtac agcatcgaga
     2101 aggccaccga gctgatgctc gacgtccatc agaagggccg agcggtcgtg tcgaacggat
     2161 cccgggagaa agccgagctc gacgtgttcc ggctccacga gcacggcctg tgggccacca
     2221 tgcagcaaga ctgagcgacc gcccatgagc agagacggtt tcggcttctt cgacaggcgc
     2281 tggttccgac ccgccgatgc cggcggcttc gacgtcgcgg tcccgagcgg ggtgcgggcc
     2341 atcctcggcg acatggtcgg cagcctcgac gagctcctgt cgtcggacag cgagaacatc
     2401 cgtcgggtct tccccaccgc gtaccccgac gacgccgagc gcgaggccgg ctaccaggcc
     2461 ttcgtgcggg gagagctcat cgagcgccac agcgcggccg ccgaggtcct cgcctcgacg
     2521 atcgacgccg accacctcac cgaggagcag ctcacgtcgt ggatgacgac ggcgaacacg
     2581 ttgcgcctgg tcttcggcac ggcactggac gtgagcgagg acgatgatcc cgccatcgac
     2641 cccgatgacg aggacgcgcc ggctcgtgtc gcctaccagg tgctcaccga actggtcgat
     2701 gacatcgtgc aggcgctcag cacgaccctc tgacagccgg gtcgagccgc cgacggtccg
     2761 agcgtcgtcg cagcctcatg ctgcggcaca cttcgatccc gcgccaccca tcacctcgaa
     2821 gagatggtcg agctcctcgt cggagggctc ggggtcggcc aggacctcga ggggtacctg
     2881 ctccacgacg gcgtcaccga tgcacgcgct cgcctcgacc ggcagcccct cctgtgtgcc
     2941 accgagcatc acgtagctgc gtgtggccgg gagagcgatg gcatcgaggg gatcggtcac
     3001 cggatcggcg aggtcatcgg tgcccggatc gcaggaccgc aacgtgacga gatccccgtc
     3061 acggtcgatg gtggcgtc
//
LOCUS       DATDBZ010000189         6840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_39178,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000189 DATDBZ010000000
VERSION     DATDBZ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39178"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VIX62_04640"
     CDS             <1..315
                     /locus_tag="VIX62_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5859551.1"
                     /translation="GTTVAEAATTMSTKKVGSALVMEGDRLVGIFTERDVMRAVASEF
                     DAEHHQVGAFMTSDPTTIGPEADVHEGLSSMFAFGFRHMPVVEGGQVVGMVSMRDLTP
                     RD"
     gene            complement(312..503)
                     /locus_tag="VIX62_04645"
     CDS             complement(312..503)
                     /locus_tag="VIX62_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859552.1"
                     /translation="MTRESDWAVFEKACEITASAVRGTMNGLDGQQAAFVGEVFREVH
                     AALRQASDEMPDRAGKPGF"
     gene            666..1022
                     /locus_tag="VIX62_04650"
     CDS             666..1022
                     /locus_tag="VIX62_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY5859553.1"
                     /translation="MRYLLLIHGEEAAWNDATDAERRDMYQAYGAVSDQMQERGHLID
                     AAELQGAGTATLVRDRDGETLVVDGPFTETKEQLGGYFLVECDLDTAVAYAAAIPGAK
                     NGGTIEVRPVVPSSED"
     gene            1045..1395
                     /locus_tag="VIX62_04655"
     CDS             1045..1395
                     /locus_tag="VIX62_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY5859554.1"
                     /translation="MQYLLLIYADMSAFPEPTEDEQRANMEQWTDYSNWLTEKGWMRG
                     GDALKDVDQATSVRIQDGEQLVTDGPFAETKETLGGYYLLEVENLDDAIQAAGRCPGA
                     RYGTIELRPVQEMP"
     gene            1398..2654
                     /locus_tag="VIX62_04660"
     CDS             1398..2654
                     /locus_tag="VIX62_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5859555.1"
                     /translation="MRSTSEEAVDLAFRRESGRAVATLIRVLGDFDRAEDAVQEAFIV
                     ALERWPRDGVPDNPGAWITTTARNRAIDRLRREQVGRKKAEATEAMRALETMGEDETP
                     EIGDDRLRLIFTCCHPALPLESRVALTLRTVGGLSTREIANAFLSPEPTVAQRLVRAK
                     RKIRDAGIPYRVPPRDLLEERLAGVLAVLYLIFNEGYRATGGEGLLRTDLCDEAIWMS
                     RIVTTLLPDRGEPLGLLALMLLQHSRRDARTDDGGGLVLLDDQDRSRWDHAMIDDGLA
                     TLDRALALHEPGPYQVQAAIAALHARAPRPEDTDWPQIASLYAGLGAMTPSPVIELNR
                     AVAVAMADGPGAGLPILDRLGDDLEGYHLFHSARADLLRRLDRPAEAAEAYRRALALV
                     TNAAERRFLEARLAMIGDPADPTTPS"
     gene            2692..4104
                     /locus_tag="VIX62_04665"
     CDS             2692..4104
                     /locus_tag="VIX62_04665"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY5859556.1"
                     /translation="MLFPTVDFAIFFALVFLGHWLLNPKPLPWKLFMIGASYVFYAWW
                     DIHFIWLLAAISTLSQLGAMAVARQQEERAKRWTMAIAVTLTLLPLAYFKYYGFFSLN
                     LTEAANSLGLSAELPLIQVILPVGVSFFSFMAVSYIVDVYRGHTTVASWIDVFLYLSF
                     FPHLVAGPIVRPDELIPQLDVRRDPRHVDVVGASWLIFGGLFKKVVISSYLASTMVDP
                     VFGSPASYGAIDVLVAIVSYAIVIYADFSGYTDIAIGIAKLLGFQFPQNFDRPYSARS
                     LQDFWRRWHITLSRWLRDYLYIPLGGSKKGEMRTYVNIVVTMVLGGLWHGAAWTFVFW
                     GLYHGVGQAIGAWKRTHLPERTETPMLLLGQRVGTFALVCVGWVFFRSDSMATAFTML
                     GRLITGWSVSPELVNTLVLGSIALMLALQYAPRRPALKLQERLSALQPAVMGLIFAGT
                     LFVIATLGPSGVAPFIYFQF"
     gene            4140..5228
                     /locus_tag="VIX62_04670"
     CDS             4140..5228
                     /locus_tag="VIX62_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY5859557.1"
                     /translation="MPRPSDQPPERASLEEAETPRPPESRTSSHGRATMPAGKVLIVM
                     VVALLVWALLFAPTLKRSSEAQPLGTRRTISLWILGPMAAISNVLQLTKATDAVSSAL
                     GKDPDAAPGGDLDIPLPTVEPGASPSPKPTEPVTKTTPMRKPTPQERLRVVVVGDSLA
                     QGLGVYMERQFRPGLVRVSQQGRISTGLARLDYFDWLTAMEEIEDGYRPDLVIVMIGD
                     NDNQSLQSPGGGSVADIGSFEWPQGYEDRVEQMTRTAVDGGAHVAWVGLPIVNDKDRW
                     PVMQRQNEIFEQVIDRTPNALYVDTWDRFATPDGDYTDFYRHDGKVELIRATDGFHFN
                     PRGYELVSQAVTDALMDRFDLSPKAVNG"
     gene            5304..5987
                     /locus_tag="VIX62_04675"
     CDS             5304..5987
                     /locus_tag="VIX62_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859558.1"
                     /translation="MTPGIDATNASVVQLYWIPLGAGARVVRLSGRLYEAFDAWRHRR
                     PAVPLYHSALVVLAPEGRFIIEQTPIPRARERRHGVVEGPVGSKLAGHLRLFRYEVRR
                     WRDGVIPDIADAVGSPVGLSDDPARASQVVELVPLVPALVWGRDQEHTGEMWNSNSVI
                     AWLLERAGLDAVTIQPPAGGRAPGWSAGREIARRERVQTWIETSGSCANAASSSAAMN
                     STSGNRPFR"
     gene            complement(5894..>6840)
                     /locus_tag="VIX62_04680"
     CDS             complement(5894..>6840)
                     /locus_tag="VIX62_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEY5859559.1"
                     /translation="SAMAHACQMFGLECDVYMVKASYDQKPYRRIFMETFGASVVASP
                     SDKTNSGRAIREAHPDSTGSLGIAISEAVEDAATHEDTNYALGSVLGHVLLHQSVIGL
                     EAQQQMEMAGERPDVIVGCVGGGSNYAGLAYPFMADKLRGETATRFLAAEPAACPTLT
                     KGRFAYDYGDTAAIGPIVPMYTLGHDFVPAPVHAGGLRYHGDSPSLSMLVHEGHMDAV
                     AYTQNQIFEAAVLFAKTQGIIPAPEPSHAIKGAIDEALAAKDAGEERVILFNLCGHGH
                     FDMQAYDDYLNGRLPEVEFIAADEDAALAQLPEVSIQV"
BASE COUNT         1088 a         2308 c         2300 g         1144 t
ORIGIN      
        1 ggcaccaccg tggccgaggc cgccactacg atgagcacca agaaggtggg ctcggccttg
       61 gtgatggaag gtgaccgcct cgtggggatc ttcacggagc gcgacgtgat gcgcgcggtg
      121 gcatccgagt tcgacgccga acaccatcag gtcggagcct tcatgacctc ggaccccacg
      181 acgatcggac ccgaagccga tgtgcacgag gggctgtcgt cgatgttcgc gttcgggttc
      241 cggcacatgc ctgtcgtcga aggtggccag gtggtcggca tggtctcgat gcgcgacctg
      301 acgcccaggg attagaaccc cggcttcccg gccctgtccg gcatctcgtc cgacgcttgt
      361 ctcagggcgg cgtggacctc tcggaagacc tcgccgacga aggctgcctg ctgcccgtcc
      421 agcccgttca tcgtccctcg tactgcggac gcggtgatct cgcatgcctt ttcgaacacg
      481 gcccaatcgg actctcgtgt catgggtgaa cctccatcag atcgatcgtc gacgttgatg
      541 gcgtcaggct accggaccca ccgcatggga cgggtctttc ggtcaggatg tcgatatcgg
      601 gcggcgtccg tacgtcgcta gggtgaacgc ggagcgcgga gctccgccga cctgggaggt
      661 gcacgatgcg ctacctgctg ttgatccatg gcgaggaagc cgcgtggaac gacgccaccg
      721 acgccgaacg acgcgacatg taccaagcgt acggagcggt ctccgaccag atgcaggaac
      781 gcggccacct catcgatgcc gccgaactcc agggcgccgg caccgccacg ctggtgcgtg
      841 atcgcgacgg cgagaccctg gtggtcgacg gaccgttcac cgaaacgaag gaacagctcg
      901 gcggatactt cctggttgag tgcgacctcg acaccgcggt cgcctacgcc gcggcgatcc
      961 cgggcgccaa gaacggcggc acgatcgagg ttcgtcccgt cgtcccatcg tccgaggact
     1021 gaccgacgcg aaaggagcac gacgatgcag tacctgttgc tgatctacgc cgacatgagc
     1081 gccttcccgg agccgaccga agacgaacag cgcgcgaaca tggagcagtg gaccgactat
     1141 tcgaactggc tcaccgagaa gggctggatg cgtggcggcg acgccctcaa ggacgtcgac
     1201 caggccacca gcgtccggat ccaggatggg gagcagctcg tcaccgatgg gccgttcgcc
     1261 gagaccaagg aaacgctggg cggctattac ctgctcgagg tcgagaacct ggacgatgcg
     1321 atccaagcgg cgggacgttg ccccggagcc cgatacggca cgatcgaact gcgcccggtc
     1381 caggagatgc cgtaggcgtg aggtcgacgt cggaggaggc cgtcgacctg gcgttccggc
     1441 gcgaatccgg acgcgcggtc gcgaccttga tccgggtgct gggcgatttc gatcgcgccg
     1501 aggatgcggt gcaggaggcc ttcatcgtgg ccctggaacg ctggccccgc gacggggtgc
     1561 cggacaaccc cggcgcctgg atcaccacca cggctcgcaa ccgggcgatc gaccggttgc
     1621 ggcgcgagca ggtgggacgg aagaaggctg aggcgaccga agcgatgcgc gccctcgaga
     1681 ccatgggtga ggacgagacg cccgagatcg gtgacgaccg tctccgattg atcttcacgt
     1741 gttgccaccc ggcgctgccg ctggaatcac gcgtcgcgtt gaccttgcgc accgtcggcg
     1801 gattgtccac gcgcgagatc gccaacgcgt tcctgtcgcc ggagcccacg gtggcgcagc
     1861 gcttggtgcg agccaagcgc aagatccgcg acgccgggat cccctaccgg gtgccaccgc
     1921 gcgatctgct cgaggaacgt ctcgcgggcg tgctcgccgt gctctatctg atcttcaacg
     1981 agggataccg cgcgaccggt ggcgaagggt tgctccgcac cgatctgtgc gacgaagcga
     2041 tctggatgag ccggatcgtc acgacgctcc ttcccgatcg cggcgaaccc ctggggctgc
     2101 tcgccttgat gctgctccag cattcgcgac gcgacgcgcg caccgacgat ggcggcgggc
     2161 tggtgctgct cgacgatcag gaccgatcgc ggtgggatca cgcgatgatc gatgacggac
     2221 tcgccacgct cgaccgggcc ctcgccctgc acgaacccgg tccgtatcag gtgcaagccg
     2281 ccatcgcggc gctccatgcc cgcgcgccgc ggccggagga cacggactgg ccgcagatcg
     2341 cctccctgta cgccggcctc ggcgccatga cgccttcgcc ggtcatcgag ctcaatcggg
     2401 ccgtcgctgt ggcgatggcc gatgggccgg gcgccggatt gcccatcctc gatcgcctcg
     2461 gcgacgacct cgagggctac cacctgttcc actcggcgcg cgcggatctg ctgcgccggc
     2521 tcgatcgtcc ggccgaggcc gcggaggcct atcgacgcgc cctcgcgctg gtgaccaacg
     2581 cggccgagcg ccggttcctc gaggcgcgcc tggcgatgat cggcgatccg gcggacccga
     2641 ccaccccttc gtaggcccgc gggcggctct ctgcgctagg gttctgcggc gatgctcttc
     2701 cctaccgtcg atttcgcgat cttcttcgcc ctggtcttcc tgggccactg gctgctgaac
     2761 ccgaaaccct tgccctggaa gctgttcatg atcggggcga gctacgtctt ctacgcatgg
     2821 tgggacatcc atttcatctg gttgctcgcc gcgatctcga cgctcagcca gctcggcgcc
     2881 atggcggtgg cgcgccagca ggaggaacgc gcgaagcggt ggacaatggc gatcgcggtg
     2941 acgctgacgc tgctcccgct cgcctacttc aagtactacg ggttcttctc gctcaacctc
     3001 accgaggcgg ccaactcgct ggggctcagc gccgagctgc cgctgatcca ggtgatcctg
     3061 ccggtcggcg tgtcgttctt ctcgttcatg gcggtgagct acatcgtcga tgtgtaccgg
     3121 ggtcacacca cggtcgcgag ctggatcgac gtgttcctgt acctgtcgtt cttcccccac
     3181 ctggtggcgg ggccgatcgt gcgccccgac gagctgatcc ctcagctgga cgtgcggcgc
     3241 gaccctcgcc acgtcgacgt ggtgggcgcg tcgtggctga tcttcggcgg gttgttcaag
     3301 aaggtggtga tctccagcta cctggcgagc acgatggtgg atccggtgtt cggcagcccc
     3361 gcgtcctacg gcgcgatcga cgtgctggtc gcgatcgtgt cctacgcgat cgtgatctac
     3421 gccgacttca gcggctacac cgacatcgcc atcggcatcg ccaagctcct cgggttccag
     3481 ttcccccaga acttcgaccg gccttacagc gcccggagcc tccaggactt ctggcgaagg
     3541 tggcacatca cgctgtcccg ctggctccgc gactacctgt acatcccgct cggcggttcg
     3601 aagaagggcg agatgcgcac gtacgtgaac atcgtcgtga cgatggtctt gggcggcctg
     3661 tggcacggcg ccgcgtggac gttcgtcttc tggggtctgt atcacggcgt cggccaggcg
     3721 atcggcgcat ggaagcgaac gcatctgccc gaacgcacgg agacacccat gctcctgctg
     3781 ggtcaacgcg tgggcacgtt cgccttggtg tgcgtgggct gggtcttctt ccgttccgat
     3841 tccatggcga ccgcgttcac gatgctggga cgcctgatca ccggctggag tgtttccccg
     3901 gagctggtga acacactggt cctggggtcg atcgcgttga tgctcgcgct gcagtacgcc
     3961 ccgcgccggc cggcgctgaa gctccaggaa cgcctctccg ccctgcaacc cgcggtgatg
     4021 ggattgatct tcgctggcac actgttcgtg atcgcgaccc tcggcccgag cggggtcgcg
     4081 ccgttcatct acttccagtt ctgaccgaga gccgaccaag aagccaccaa gaagccgaga
     4141 tgccaagacc cagcgaccag ccgcccgaac gcgcgtcact cgaggaggcc gagacgcccc
     4201 gtcctcccga gtcgcggacg tcgtcgcacg gccgggcgac catgccggcg ggcaaggtgc
     4261 tgatcgtgat ggtggtggcg ctcctcgttt gggctctgct ctttgcgccg acgttgaagc
     4321 gatcgtccga ggcacagccg ctggggaccc gccgaaccat ctcgttgtgg atcctgggtc
     4381 cgatggcggc gatcagcaac gtcctgcagt tgacgaaggc caccgatgcc gtctcgagcg
     4441 cgctcggcaa ggacccggat gccgcgccgg gcggcgacct cgacatcccg ctgccgacgg
     4501 tcgagccggg cgcctcaccc tcgcccaagc cgaccgagcc cgtcacgaag accacgccga
     4561 tgcgcaagcc cacgccgcag gaaaggctcc gggtggtcgt ggtgggcgat tcactggccc
     4621 aggggctcgg cgtctacatg gagcgacagt tccggccggg gctcgtccgc gtctcccagc
     4681 agggccgcat ctcgaccggc ctggcgcgtc tcgactactt cgactggctc accgcgatgg
     4741 aggagatcga agacggctac cggccggacc tggtgatcgt gatgatcggg gacaacgaca
     4801 accagagcct gcagagccct ggtggcggca gcgtcgcgga catcggcagc ttcgaatggc
     4861 cgcagggcta cgaagaccgg gtcgaacaga tgacgcggac cgccgtcgac ggaggtgcgc
     4921 acgtcgcctg ggtgggcctg cccatcgtga acgacaagga ccgatggccc gtgatgcagc
     4981 gtcagaacga gatcttcgag caggtgatcg accgcacccc gaacgcgctc tacgtcgaca
     5041 cctgggatcg gttcgccacc cccgacgggg actacaccga cttctaccgc cacgacggca
     5101 aggtcgagct gatccgggcc accgacgggt tccacttcaa cccccgcggc tacgagctgg
     5161 tgtcacaggc cgtcaccgac gcgttgatgg accggttcga cctctcgccg aaagccgtca
     5221 acggctaggt ccgatcggtc ctgccggagg aggggcgtcc ggccctcgga tcgaaccagt
     5281 ggccgaggtg aactgtccgg gtgatgactc cagggatcga cgcgacgaac gcatccgttg
     5341 tccagctgta ctggatcccg ttgggcgcgg gagcgcgcgt ggtgcgcctg tccgggaggt
     5401 tgtacgaagc gttcgatgcg tggcgtcacc gacgccctgc agttccgctc tatcactcag
     5461 ccctcgtcgt cctcgcccct gagggccgat tcatcatcga gcagaccccg atccctcggg
     5521 ctcgggagcg acggcacggc gtggtcgaag gaccggtcgg ctcgaagctg gcggggcacc
     5581 tgcggctctt ccggtacgag gttcgccgat ggagggacgg cgtcatcccg gacatcgcgg
     5641 acgcggtggg gagccccgtt ggcttgagcg acgatcccgc acgggcatcc caggtcgtgg
     5701 aactcgtgcc gctggtcccg gcactggtct ggggccgcga ccaggagcac acgggcgaga
     5761 tgtggaactc gaactccgtg atcgcctggc tgctggagcg tgcaggactg gatgccgtca
     5821 cgatccaacc accggccggc ggccgcgcac ccggatggtc ggccggacgc gagatcgcac
     5881 ggcgcgagcg ggttcagacc tggatcgaga cttccggcag ctgcgccaac gcggcgtcct
     5941 catcggcggc gatgaactcc acctcgggca accgcccgtt cagatagtcg tcgtaggcct
     6001 gcatgtcgaa gtgcccgtga ccgcagaggt tgaacaggat cacgcgctcc tcgccggcgt
     6061 ccttcgcggc gagcgcctcg tcgatcgcgc ccttgatcgc gtggctcggc tctggcgccg
     6121 ggatgatccc ctgggtcttc gcgaacagca cggcggcctc gaagatctgg ttctgcgtgt
     6181 aggccaccgc gtccatgtgg ccttcgtgga ccagcatcga caggctcggc gagtcgccgt
     6241 ggtagcggag accgcccgca tgcaccggcg ccggcacgaa gtcatggccc agcgtgtaca
     6301 tcggcacgat cggaccgatc gcggcggtgt ccccgtagtc gtaggcgaac cgtcccttcg
     6361 tcagcgtggg acaggccgcg ggctcggcgg ccaggaaccg ggtggcggtc tccccacgga
     6421 gcttgtcggc catgaacgga tacgccaggc ccgcgtagtt cgaaccgccg ccgacgcacc
     6481 cgacgatgac gtcgggacgc tcgcccgcca tctccatctg ctgctgggcc tcgagcccga
     6541 tgaccgactg gtgcaggagc acgtgtccca gcacgctgcc cagcgcgtag ttcgtgtcct
     6601 cgtgcgtcgc ggcatcctcc accgcttcgc tgatcgcgat gccgagggaa ccggtcgagt
     6661 cgggatgcgc ctcgcggatc gcgcggccgc tgttcgtctt gtcgctcggc gacgccacga
     6721 cgctggcgcc gaacgtctcc atgaagatgc ggcggtacgg cttctggtcg tacgacgcct
     6781 tcaccatgta cacgtcgcac tcgagcccga acatctggca cgcgtgcgcc atggccgagc
//
LOCUS       DATDBZ010000190         6324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2308353, whole genome shotgun sequence.
ACCESSION   DATDBZ010000190 DATDBZ010000000
VERSION     DATDBZ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308353"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VIX62_04685"
     CDS             <1..825
                     /locus_tag="VIX62_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859560.1"
                     /translation="QMAQAMRHIRGEDAPEGEADEAIAVGEAAPLEDRRATSPFMKEL
                     SFDARKSLTRIMGITSILKHKKDAKEQAQLVRQLTAHTRRLEHIVSDLGDAERLVHGT
                     IELTVRRTDLETLVRRVVEESGVDADHEVVIHADRLVVAVDPLRTEQILAGLLRASGD
                     RTPAKKTIVVRLEQHEEGALISVSDPEPSSDASMSPVVQRFAEAQAGWATVEGREEGG
                     SAFKVYLPDGAGIGEPAGATRDVKVVVDTPTDDWEHSDGSSMLVQELHRLSTAEEN"
     gene            complement(838..2019)
                     /locus_tag="VIX62_04690"
     CDS             complement(838..2019)
                     /locus_tag="VIX62_04690"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5859561.1"
                     /translation="MAERAVIVAGARTPIGKFRGSLAGFKAVDLGAAAMAGALGRSGV
                     APEQIEYSIMGHVLQAGQGQITSRQAAIAAGIPKEIPAITVNKVCLSGTSAIAIADQM
                     IRAGDLEIAVAGGMESMTNAPFVLPNAREGSRLGDTEMLDSMIHDGLWCAFDDRHMGE
                     GTDAISAELGITREDQDAWAARSQQRARDAWDAGRLGGEVVPVAVPQRKGDPVLFERD
                     EGIRETTVEVLAKLRPAFTPDGTVTAGNASQISDGGAAVVVTSADVAERMNLEPIAEI
                     VAYGMSADRYASLHTVPALALEKALKRAGLAASDLGLLEVNEAFAAVSLHTARTLDID
                     ESLVNVNGGAVALGHPIGASGTRIVLTLALEMQRRGVDLGAAAICGGGGQGDALILRR
                     L"
     gene            complement(2063..3883)
                     /locus_tag="VIX62_04695"
     CDS             complement(2063..3883)
                     /locus_tag="VIX62_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5859562.1"
                     /translation="MPNKETASDTTALLQGSQLLQKGRADVFRQRERTKFKRIIRAIV
                     VIGLIDLYLWYRYSSGRPLTLPSLPDDWVFLLFPLLILIPVLLMVALPLINGRSPHIT
                     VYPEQVESGLTDIKGLDTQVDEVVRSLDVFLGYATFREELGGTPRRGILFEGPPGTGK
                     TYLAKAMAKQAGVPFLFISAPAFQSMWQGMTAYRIRSFFKALRKAARKEGGAIGFIEE
                     IDAIGMSRGGGNNATPDGIARATVENFGMGDSGSMVNELLIQMQSFDQPPQRTRLRAK
                     MIGWVNAYLPTDRQLTAIKSEYHNILLIAATNRGDSLDAALVRPGRFDRRLYFDVPTK
                     QGRVDLIDWFLDRKSHHQQLDDPQTRERIAYETFGYTPVMIEHLFDEGMLVALREGRR
                     QMNFDDVMEAKLNEEIGLKQPVVYTEQDREAVATHEAGHATVAYFVGQNRRLEVLSII
                     KRRGSLGMLAHGDSEERFTRTRSEIESGIAIALGGLVAEELCIGDAGTGPAGDLAHAT
                     SLAAGMVGSFGMAGSLISYDAVTQGPIGGANLVGKVLADSEGRQRVDDILEAQREKVI
                     AVLEANRDVHAALRDALIARDELIRDDILEVIEQALSARA"
     gene            4071..4448
                     /locus_tag="VIX62_04700"
     CDS             4071..4448
                     /locus_tag="VIX62_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859563.1"
                     /translation="MARKKQPPIGAADKRTIGALLRELRRGAGYRSVDKAAEVPACPA
                     STATIYAYERGGLVPSLAQFLELVEFYVLETPPMATGAKPEGDLRTMGVAAVTRALTL
                     PAYHVAQANDLVARMQPDLGDHP"
     gene            4445..4855
                     /gene="mce"
                     /locus_tag="VIX62_04705"
     CDS             4445..4855
                     /gene="mce"
                     /locus_tag="VIX62_04705"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEY5859564.1"
                     /translation="MSIELTDLDHVGLAVADLEASVAEYSRLLGVEPTHRERVDDQGV
                     EEVLFPVGSSYIQLLGALGADTPVGRFLASRGPGMHHVAYRVDDIEGALAHLRAEGAQ
                     LIDEAPRAGSRNTLIAFVHPRTMGGVLVELVQER"
     gene            complement(4898..6139)
                     /gene="ccrA"
                     /locus_tag="VIX62_04710"
     CDS             complement(4898..6139)
                     /gene="ccrA"
                     /locus_tag="VIX62_04710"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEY5859565.1"
                     /translation="MGKDLYDIGELPPLGDVPAQMHAQLVRPDRYGDPEKAIQDEVID
                     VPELGPNDALVLVMAAGVNFNNVWAARGVPVDVTKTQARWGEPTDFHIVGSDASGVVW
                     AIGDQVSNVQVGDRVVVHAGQWDLDDPHVKAGKDPGLAASFRVWGYDTCWGSFAQFTK
                     VQAHQCLPKADFLTWEEAAAPTLTGSTAYRMLFGWPPHTVEPGDVVLVWGASGGLGSL
                     AVQLVANAGGRAVAVVGSDEKGEYAKSLGAVGYVNRKAFPHWGVPPAWDSAEWKDWFG
                     GAKDFGKAIWEALGEKVSPRIVFEHPGQDTIPTSIFVADRGGMVVICAGTSGYDTMVD
                     VRYLWYMQKRYQGSHLFNDDQAQAFNDLVVQGRIQTTLGAAYPYDQSGHVHQLMGDGG
                     LPEGNVSVLINAPTEGLTDLP"
BASE COUNT          997 a         2177 c         2151 g          999 t
ORIGIN      
        1 cagatggccc aagcgatgcg gcatatccga ggcgaggatg cgcccgaggg cgaggccgac
       61 gaagccatcg ccgtgggcga ggccgctccc ctggaggacc ggcgcgcgac cagcccgttc
      121 atgaaggagc tgtcgttcga cgctcgcaag tccctcaccc ggatcatggg catcacgtcg
      181 atcctcaagc acaagaagga cgcgaaggag caggcacagc tggtccgaca gttgacggcc
      241 cacacccgtc gtctcgagca catcgtgtcg gacctcgggg acgccgagcg cctcgtgcac
      301 ggcacgatcg agctcaccgt ccgccgcacc gacctggaga cgctggtgcg ccgggtggtc
      361 gaggaatccg gcgtcgacgc cgaccacgaa gtcgtgatcc acgccgaccg gctggtggtg
      421 gcggtcgacc cgctgcgcac cgagcagatc ctggccggat tgcttcgagc gagcggtgac
      481 cgcacgccgg cgaagaagac gatcgtggtc cggctcgagc agcacgagga aggcgcgttg
      541 atctcggtct cggaccccga accctcgtcc gatgcttcga tgagcccggt ggtgcagcgc
      601 ttcgccgaag cgcaggcagg ttgggcgacg gtcgagggcc gggaagaggg gggttctgcg
      661 ttcaaggtgt acctgccgga cggggccggg atcggtgagc ctgcgggggc cacgcgcgac
      721 gtcaaggtcg tcgtggacac gcccacggac gactgggagc acagcgacgg atcctcgatg
      781 ctggtgcagg aactgcaccg gttgtcgacc gccgaggaga actgagcgag gcgaccgtca
      841 gaggcgacgc aggatcaacg cgtcgccctg cccgccgccg ccgcagatcg ccgcggcgcc
      901 caggtccacc cctcgccgct gcatctccaa cgccagggtg agcacgatcc gcgtgccgct
      961 cgccccgatc ggatgaccga gcgccacggc cccgccgttg acgttcacca ggctctcgtc
     1021 gatgtccaag gtccgggcgg tgtggagcga gaccgccgcg aaggcctcgt tgacctcgag
     1081 caggccgagg tcggaggccg cgagcccggc ccgcttcagc gccttctcca gcgcgagcgc
     1141 gggcaccgtg tggagcgagg cgtagcggtc ggccgacatg ccataggcaa cgatctcggc
     1201 gatcggctcc aggttcattc gctcggccac gtcggcgctc gtcacgacga cggcggcgcc
     1261 cccgtcggag atctgcgagg cgttacccgc ggtgaccgtg ccgtcgggcg tgaaggccgg
     1321 tcgcagcttc gcgagcacct cgacggtggt ctcgcggatc ccctcgtctc gctcgaagag
     1381 caccggatca cctttccgct gcggaaccgc gacgggcacg acctcgcctc ccaggcggcc
     1441 cgcatcccag gcatcacgcg cgcgctgctg tgaacgcgcc gcccacgcgt cctggtcctc
     1501 acgggtgatc ccgagttcgg cgctgatcgc gtccgtgccc tcccccatgt gccggtcgtc
     1561 gaacgcacac cacaagccgt cgtggatcat ggaatccagc atctcggtgt cgcccagccg
     1621 cgagccctca cgcgcgttcg gcaggacgaa gggcgcgttc gtcatcgact ccatgccgcc
     1681 ggcgaccgcg atctcgaggt caccggcgcg gatcatctgg tcggcgatcg cgatggcgga
     1741 cgtgcccgac aggcagacct tgttcaccgt gatggcgggg atctccttgg ggatgccggc
     1801 ggcgatcgcc gcctgccgcg aggtgatctg gccctgaccc gcctgcagga cgtgccccat
     1861 gatcgagtac tcgatctgct cgggggccac accggatcgc cccagcgccc cggccatcgc
     1921 ggccgcgccc aggtcgacgg ccttgaaccc cgccagggac ccgcggaact tcccgatggg
     1981 ggttcgagcc cccgcaacga tcaccgctcg ctcagccatg cctccagtct agaggcggcc
     2041 cgcgaacaac cgtgagtgac gctcaggcgc gggcggacag ggcctgttcg atgacctcga
     2101 ggatgtcgtc ccggatcagc tcgtcgcggg cgatcagcgc atcacgcaat gcggcgtgca
     2161 cgtcgcggtt ggcttcgagt acggcgatga ccttctcgcg ctgcgcctcg aggatgtcgt
     2221 ccacgcgctg gcggccctcg ctgtcggcga ggactttgcc cacgaggttc gcgccaccga
     2281 tcggaccctg cgttacggcg tcgtaggaga tcagcgatcc cgccatgccg aacgatccga
     2341 ccatgcccgc cgcgaggctg gtggcgtgcg cgaggtcgcc cgcgggaccc gtgccggcat
     2401 caccgatgca cagctcctcg gcgaccagac cccccagggc gatcgcgatc cccgactcga
     2461 tctcgctccg cgtccgggtg aaccgctcct cggaatcacc gtgcgcgagc atgccgagcg
     2521 atccacgccg cttgatgatc gacagcactt cgaggcgccg gttctgtccc acgaagtagg
     2581 cgaccgtggc atgaccggcc tcgtgcgtgg cgacggcttc gcgatcctgc tcggtgtaga
     2641 cgaccggctg tttcagcccg atctcctcgt tgagcttggc ttccatcacg tcgtcgaagt
     2701 tcatctggcg acggccttca cgcaacgcga cgagcatgcc ctcatcgaag aggtgctcga
     2761 tcatcaccgg ggtgtagccg aacgtctcat aggcgatgcg ctcccgggtc tgcggatcgt
     2821 ccagctgctg gtggtggctc ttgcgatcga ggaaccagtc gatcaggtcc acgcggccct
     2881 gcttggtggg gacgtcgaag tagagacgcc ggtcgaaccg gcccggacgc accagggcgg
     2941 cgtcgaggga gtcgccgcgg ttcgtggccg cgatcagcag gatgttgtgg tactcggact
     3001 tgatggccgt caattgacgg tcggtgggca gataggcgtt gacccacccg atcatcttgg
     3061 cgcgaagacg ggtgcgctgt ggcggctggt cgaacgactg catctggatc aagagctcgt
     3121 tcaccatgga acccgaatcg cccatgccga agttctccac ggtggctcgc gcgatcccat
     3181 cgggggtggc gttgttgcca ccaccacgcg acataccgat cgcgtcgatc tcctcgatga
     3241 agccgatcgc gcccccttcc ttgcgcgcgg ccttccgcag ggctttgaag aacgatcgga
     3301 tgcgataggc ggtcatcccc tgccacatcg actggaacgc gggggccgag atgaacagga
     3361 acggcacgcc cgcctgcttg gccatggcct tcgccaggta ggtcttgccc gttccgggcg
     3421 gtccttcgaa caggatgcct cgccgtggag tgccgccgag ctcttcacgg aaggtggcgt
     3481 agcccaggaa cacgtcgagg gaacggacca cttcgtcgac ctgggtgtcg agccccttga
     3541 tgtcggtgag gcccgattcg acctgttcgg ggtagacggt gatgtggggt gagcgcccgt
     3601 tgatcagcgg caacgcgacc atgagcaaca ccgggatcaa gatcaggagc gggaagagca
     3661 ggaacaccca gtcgtcgggc agggacggca gcgtgagtgg gcggccggac gagtagcggt
     3721 accagaggta gagatcgatc agcccgatca cgacgatcgc gcggatgatc cgcttgaact
     3781 tcgtgcgttc gcgctggcgg aaaacgtcgg cgcgaccctt ctgcagcaac tgggagccct
     3841 gcaacagggc ggtggtgtcg gaagccgttt ccttgttcgg cacgggcctt gctccttgct
     3901 cgggtggcgg gtccccctgt gatccgcctg cgagccaggc tacgccgacc ggcctccccc
     3961 ggatatcggt cctacggccc agacggggga ccgaatggcc ccgtggccca ggcgcgcaac
     4021 gggtttcgcg ggccgtgcgg cggtccgtac ccacccggta gggttcggcc gtggccagga
     4081 agaagcagcc gccgatcggt gcggcggaca agcgcacgat cggggcgctc ctgcgcgagc
     4141 tccggcgtgg agcgggctac cgatcggtgg acaaggcagc cgaggtcccc gcgtgccccg
     4201 cgtccaccgc cacgatctac gcctacgagc gtggtgggct ggtgccgtcc ctggcccagt
     4261 tccttgagtt ggtcgagttc tacgtgctcg agacacctcc gatggcgacc ggggccaagc
     4321 ccgaaggcga cctgcggacg atgggcgtgg cggccgtcac gcgcgcgctc accctgcccg
     4381 cgtatcacgt ggcgcaggcg aacgatctcg tggcccgcat gcaacccgac ctcggggatc
     4441 acccgtgagc atcgaactca ccgacctcga ccatgtaggc ctcgccgtcg ctgacctcga
     4501 agcatcggtc gccgagtact cgcgccttct cggggtcgaa ccgacccacc gggagcgcgt
     4561 cgacgatcag ggcgtcgagg aagtgctgtt cccggtcggc tcctcctaca tccaactgtt
     4621 gggtgcgctg ggggccgaca ccccggtcgg acggttcctc gcctcccgag ggcccggcat
     4681 gcaccacgtg gcctaccgcg tggacgacat cgaaggtgcg ctcgctcacc tgcgtgccga
     4741 gggcgcccag ctgatcgacg aagcaccgcg cgcgggttcg cgcaacacgc tgatcgcgtt
     4801 cgtgcacccc cgaacgatgg gcggcgtcct ggtcgagttg gtccaggaac gctgagcgtg
     4861 ggctaggacc cggaaacgcc gaggttcgcg tcggcggcta gggcaggtcg gtgaggccct
     4921 cggtgggcgc gttgatcaac acgctcacgt tgccttcggg caggccgccg tcgcccatca
     4981 gctgatgcac gtgaccgctc tggtcgtagg ggtaggcggc gcccaacgtg gtctggatcc
     5041 tgccctggac caccaggtcg ttgaatgcct gggcctgatc gtcgttgaac agatgcgagc
     5101 cctggtagcg cttctgcatg taccagaggt agcgaacgtc caccatggtg tcgtagccgc
     5161 tggtccccgc gcagatcacg accatgccgc cgcggtcggc gacgaagatg gatgtcggga
     5221 tcgtgtcctg tcccgggtgc tcgaagacga tgcgggggct gaccttctcc cccagcgcct
     5281 cccagatggc cttgccgaag tccttggcgc cgccgaacca gtccttccac tcggccgaat
     5341 cccacgccgg gggcacgccc caatgcggga acgccttgcg gttgacgtaa ccgaccgcgc
     5401 cgagcgactt cgcgtactcg cccttctcat cgctccccac cacggccacc gcgcgcccgc
     5461 cggcgttggc gaccaactgc acggcgaggg agccgagccc gccgctggcg ccccacacca
     5521 gcacgacgtc gccgggttcc acggtgtgcg gaggccagcc gaagagcatg cggtaggcgg
     5581 tcgacccggt gagggtgggg gccgctgctt cctcccacgt gaggaagtcg gccttgggca
     5641 gacattggtg cgcctgcacc ttggtgaact gggcgaacga gccccagcag gtgtcgtagc
     5701 cccagacacg gaacgaggcc gccaggcccg ggtccttgcc cgccttcacg tgcgggtcgt
     5761 ccaggtccca ctggccggcg tgcaccacga cgcggtcgcc cacctggacg ttcgagacct
     5821 gatcgccgat cgcccacacg acgccgctcg cgtccgaacc cacgatgtgg aagtcggtgg
     5881 gttcgcccca ccgcgcctgc gtcttcgtga catcgaccgg gaccccgcgt gccgcccaga
     5941 cgttgttgaa gttcaccccg gccgccatca ccagcaccag ggcgtcgttc ggccccagtt
     6001 cgggaacgtc gatgacctcg tcctggatgg ccttctcggg atcgccgtag cggtcgggac
     6061 gcaccagctg cgcgtgcatc tgcgccggca cgtcacccaa cggcggaagc tctccgatgt
     6121 cgtacaggtc cttgcccacg gctctcaccc ttttccggtg gtgacgacga acacggtcgg
     6181 gcgaacccta gcaagccgtg aacgaagcgc gagttcctgg agcgttaccc ggcgtcgggc
     6241 cgcgtgggcg gaacgtcctg cgccggtgcg ggcggaagtg ccgaacccat gccgtccgtc
     6301 gccgaaccgg acgcgtcctt gccg
//
LOCUS       DATDBZ010000191         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1497203, whole genome shotgun sequence.
ACCESSION   DATDBZ010000191 DATDBZ010000000
VERSION     DATDBZ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497203"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VIX62_04715"
     CDS             complement(<1..434)
                     /locus_tag="VIX62_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008376450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEY5859566.1"
                     /translation="MDIPSPARIMVEIQKAATRGHRLLLQSPLGPLVSTTPFGRFLIL
                     HTTGRRSGEPRKTPLSYTKDGDTYIVIGSDGGSPHNPDWYLNLVDEPNAEIDVGGQRS
                     RVHAETVTGDERDGLWRRAVASYGGYAGYQARTDRQIPVVRLS"
     gene            complement(575..1720)
                     /locus_tag="VIX62_04720"
     CDS             complement(575..1720)
                     /locus_tag="VIX62_04720"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEY5859567.1"
                     /translation="MDVRLRIAVASDGVNVAAFCREHGLSRETFYVWRRRYLAEGLEG
                     LEPRSRAPETSPQRIGLELEEAIVALRKELDDRGVDAGAGTIQWHLGRQGRRAVPSEA
                     TIWRLLVRRGFVVREPRKRPRSSWRRFAADAPNELWQADCIDWVIATGVVRILSFLDD
                     HSRIALRVRALAEATTDATWLTFCEATAAWGVPLGQLSDNGLNFSGRLRGFEVRFEIE
                     LRAIGVVPKTSRPYHPQTCGKVERFQQTLKKWLRRQPLAADLDELQAQLDTFVAYYNH
                     QRPHRGIGRRTPAEAWAATPPAINLGVALPGPAHATTVVVGDNGVVRVGRYRIAIGNQ
                     WRGHTARVHHDDTHAAVFIDHKLVRALALDPSRSYQPSGRPRGRRPT"
BASE COUNT          314 a          646 c          760 g          342 t
ORIGIN      
        1 gagagccgca cgactgggat ctgtcgatcc gtgcgcgcct ggtatccggc gtagcctccg
       61 taggacgcaa ccgctcgacg ccacaggccg tcgcgctcgt cgccggtgac ggtctcggca
      121 tgaacccgtg agcgctggcc gccgacgtcg atctcagcat tcggctcatc cacaaggttg
      181 aggtaccagt cggggttgtg gggagaccct ccatcggagc cgatgacgat gtaggtgtcg
      241 ccatccttgg tgtacgacag tggcgtcttg cgcggctcgc ccgaacggcg accggtggtg
      301 tgcaggatca ggaaccgacc gaacggcgtc gtcgacacca gtggtccgag cggggactgc
      361 agcaacagcc ggtggccacg ggtggccgcc ttctggatct cgaccatgat gcgggcgggc
      421 gacggaatgt ccatgctctg actccttcag cgtcgtgctc ctgcgggcaa gacagggtac
      481 gacgaatctg ggcgcgcagg gtctcgaatc tgcgtatgtt gtgtcgcgcg acatgtctga
      541 cacttctgtc gcgagacatc cctgacaggt gaggttaggt cggtcggcgg ccgcggggtc
      601 gtccggaggg ctggtaggac ctggacgggt cgagcgcgag ggcgcgtacg agtttgtggt
      661 cgatgaacac ggcggcgtgg gtgtcgtcgt ggtggacgcg ggctgtgtgg cctcgccact
      721 ggttgccgat ggcgatgcgg tagcggccga cccgtacgac accgttgtcg ccgaccacga
      781 cggtggtggc gtgggcgggt ccgggcaggg cgacaccgag gttgatcgct ggtggggtcg
      841 ccgcccaggc ttcggcgggg gtgcgacgtc cgatgccgcg gtgggggcgt tgatggttgt
      901 agtaggcgac gaaggtgtcg agctgcgctt ggagctcgtc gagatcggcg gcgaggggct
      961 gtcggcggag ccacttcttg agggtctgct ggaagcgttc gaccttgccg caggtctggg
     1021 ggtggtaggg ccgtgacgtc ttggggacca ccccgatggc tcgtagctcg atctcgaagc
     1081 ggacctcgaa gccgcgcaga cgacccgaga agttgaggcc gttgtcgctg agctggccca
     1141 gcgggacacc ccaggcggcg gtggcctcgc agaacgtgag ccaggtggcg tcggtggtcg
     1201 cctcggcgag tgctctgacc cgcaacgcga tccgcgagtg atcatcgagg aagctgagga
     1261 tccggaccac accggtggcg atcacccagt cgatgcagtc ggcctgccag agctcgttgg
     1321 gggcgtcggc ggcgaagcgt cgccacgagc tgcggggccg cttgcggggc tcgcgcacca
     1381 cgaacccccg ccgcaccagc agccgccaga tcgtcgcttc cgacggcacc gcccgccgac
     1441 cctgacggcc caggtgccac tggatcgtgc ccgctccggc atccacaccg cgatcgtcga
     1501 gttccttacg gagcgccacg atcgcctcct cgagctcgag accgatccgc tgagggctgg
     1561 tctcaggggc ccgcgatcgg ggctcgagcc cctcgagccc ttcggccaga tagcgccgcc
     1621 tccacacgta gaaggtctcg cggctcagcc catgttcacg acagaacgcc gccacgttca
     1681 cgccatcgga cgccaccgcg atcctcaacc ggacatccat cgggatcacc tctctggcca
     1741 tggccaacga tggccacgac ccacgcaggt gtcagggatg tcgcgcgaca gcaagtgtca
     1801 gggatgtcgc gcgaaaggac attgcggaaa ccgagacgga tcccgcccag atacggatcc
     1861 cgcccagttg gggtgccttc gggggtccac gggggcggag cccccgtgtg tacggatccc
     1921 gcccagatgg gggtcacccg gcgggcgcgt taccgttccg ggggaggagg gggtcgaggt
     1981 ggcgcggata tcgacgtcgg gctcgccgtg ggcgatcgtg gtgctcggcg cggtcatgac
     2041 cggcttcggg gtgctcgtcc tc
//
LOCUS       DATDBZ010000192         6774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2520942, whole genome shotgun sequence.
ACCESSION   DATDBZ010000192 DATDBZ010000000
VERSION     DATDBZ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520942"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..727
                     /locus_tag="VIX62_04725"
     CDS             191..727
                     /locus_tag="VIX62_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859568.1"
                     /translation="MTTPRTDRAHSDDQVRGWFAGRVPDDWFSGPLEISADRDEILVM
                     GTIPAPDLPEDESAEARSAATGSRIAGFREDTRARRMKVAAEAEHRFGRKVSWGVICA
                     DQRAMFTTLSVPAMTRLRLAERQVLDTLVDAGVARSRSHALAWCVRLVAERQDDWLRD
                     LRQALTSVEKVRAEGPKD"
     gene            complement(763..978)
                     /locus_tag="VIX62_04730"
     CDS             complement(763..978)
                     /locus_tag="VIX62_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859569.1"
                     /translation="MVGDAEALVVDVRPFDVHGVGYVDVTVAYRDRTTATARLGRESV
                     PDDLEAGEQVITRLVANMIVSIERSTT"
     gene            1122..2423
                     /locus_tag="VIX62_04735"
     CDS             1122..2423
                     /locus_tag="VIX62_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY5859570.1"
                     /translation="MYRGGTPFRRGLATFAAIVSMGATLVAGGVTATAAGGGSSAPLP
                     HLGEPGSGPAPFPVELPATASPRAATFRWADLDNSDAWAKAAIDFVGKANDWMRDFAA
                     KPDGTVPFKPDMVQTRKYLARAAVKAFAPAADVDPAITFTDLDPTQAFYKWANIAVQR
                     GWMRRSPDGRFMPDQAVTTGVLHSVLIDALGMWGIARQLDGLHTRDGAHFQTPERFGA
                     LMLGLRLGLRYNNSNEAMDVGPKSPLTRAQVAYSLYKAATLPSWVVPWVSEQYDGIVL
                     PKMGPKPRAIVRWGLKAVGLPYVWGGEWGRANPPAALGGQPVPGFDCSGLAWWLLRAN
                     DGGAWDVHPPRPYAGWALPQRTSADMARFGALRYDRLLPGDLMFYDGNDDGTVDHVDV
                     YIGNGYALDSSNTPGGVTIMWVGDGWYREHFVHGRRILPSA"
     gene            complement(2424..2825)
                     /locus_tag="VIX62_04740"
     CDS             complement(2424..2825)
                     /locus_tag="VIX62_04740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY5859571.1"
                     /translation="MVEVTVHIRWRDMDNYGHVNNAVYLNYLEECRDRMVEGLFGADE
                     AWDFVLAHVGIDFRNQLTQDDGDVIVHCEVAGFGRSSIRTRERIEKADGTVAADAEAV
                     IVPRSPDGPSSRPLTEDERAVLQRAIEGAGA"
     gene            complement(3010..4653)
                     /locus_tag="VIX62_04745"
     CDS             complement(3010..4653)
                     /locus_tag="VIX62_04745"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5859572.1"
                     /translation="MRERAAATFGVLRTALRNRSLRRALVAFLLFNTQEYAVWVAVAV
                     YAFQQGGAGEAGAVLVLQLVPAAIVAPIGSVLADRIPRAKALVLGYGLQACANLVLGV
                     CLIEAPAPVAYAAAVLAASVITLTRPAHNAILPSLATTPGELTAANAASSTMDGIGTL
                     IGPLAGAVLMARWGPGGAVLAMAVVAALSVAAALGVRPDRPDEPHPSEHVSTFLSQAL
                     GGLRQARAEPDVATLLGVGGVQFVMVGMLDVFFALLAIDVLGSGESGAGVLAGGFGLG
                     LLLGAAGAVGLVGVRRLAPAVLVALLGSGAALAALSVASSMPVAVAVLALCGATIGLF
                     AVASRTLLQRRADDVVLARVFGLEEALQMIGLAVGAAAAPMLVSAFGAAGALVAAGAL
                     LVIVALGAWPRLRRIDEVTVGPGPAFDLLRRVSIFSPLPQHVQEQLTWHLIPFRVPSG
                     GVVIREGETGDRVYVVRDGTVEVTKGGVRVAELGPGDYFGEIALLRDVPRTATVTSLE
                     DVDLLALERDVFLAAVTGSVLSRRQADEEADRRLAEGDR"
     gene            complement(4785..6410)
                     /gene="groL"
                     /locus_tag="VIX62_04750"
     CDS             complement(4785..6410)
                     /gene="groL"
                     /locus_tag="VIX62_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895269.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5859573.1"
                     /translation="MAAKIISFDEDARRALERGMDQLANAVKITLGPRGRNVVLEKKW
                     GAPTITNDGVSIAKEIELDDPEEKIGAELVKEVAKKTDDVAGDGTTTATVLAQAIVKE
                     GLRNVAAGANPMSLKRGIERATELAVEAIKNQSKEVDDKAQIANVGAISAADPQIGET
                     IAEAVDKVGKDGVITVEESNTFGMELDFVEGMRFDKGYISPYFVTDSERMEAVLEDPY
                     ILIANSKISAVKDLLPVLEKVMQAGKPVLVLAEDVEGEALATLVVNKIRGTFRSASVK
                     APGFGDRRKAMLQDIAVLTGATVISEEVGLKLENATLDLLGTARKVIVTKDETTIVEG
                     AGKDEDVQGRINQIKGEIEKTDSDYDREKLQERLAKLSGGVAVIKVGAATEVELKEKK
                     HRIEDAVQSTKAAVEEGIVPGGGVALLNAQAALDKVDLEGDEATGASIVRRALEEPLK
                     QIAVNAGLEGGVIVEKVRALDPGYGLNAANGEYGDMLKFGVIDPTKVTRSALQNAASI
                     AALFLTTEAIVAEKPEKAPAMPAGGMPGGDMDF"
BASE COUNT          999 a         2476 c         2247 g         1052 t
ORIGIN      
        1 cgcgagctcc ccccggccgg cgaacgcatg cgcctcgggg gtctccagat cgctggtgag
       61 ccggctgccc ccgtatgcgc ccgggggggc ctcgtcgggc aggggctcca tggggccgca
      121 gcgtaccaac cccctcgcgc tggtctcccc ccatcgttga cgtatgtgtg taatgaagta
      181 atctcccggt atgacgacac cgaggaccga tcgcgcgcac tccgacgacc aggttcgagg
      241 atggttcgca ggacgcgttc ccgacgactg gttcagtggc ccgctcgaga tcagcgccga
      301 ccgcgacgag atcctcgtga tgggcacgat cccggctccc gacctgcccg aggacgagag
      361 cgcggaggct cggagcgcag cgaccggatc caggatcgcg gggttccgtg aggacactcg
      421 agcccggcgg atgaaggtcg ccgccgaggc ggagcatcga ttcggacgca aggtgagctg
      481 gggcgtgatc tgcgccgatc agcgggcgat gttcacgacc ctcagcgtgc cggcgatgac
      541 ccgcctgcgc cttgcggagc ggcaggtgct cgacacgctc gtcgatgccg gggtcgcccg
      601 cagccggagc catgccctcg cttggtgcgt gcgtctcgtc gcggagcgtc aggatgactg
      661 gctgcgcgac ctgcgccagg ccctgacgtc ggtcgagaag gtgcgggcag agggcccgaa
      721 ggactgaccg ccgccctgag ccttcgccct cagcctccgc cctcaggtgg tcgatcgttc
      781 gatcgagacg atcatgttcg cgacgaggcg ggtgatgacc tgctcccccg cctctagatc
      841 gtcgggcaca ctctctcgcc cgagacgggc ggtcgccgtc gtccggtctc ggtacgccac
      901 ggtgacatcg acgtagccca ccccgtgcac atcgaagggg cgcacatcga ccaccagcgc
      961 ctcggcatca ccgaccatga gccgagagta ccgcaaggcc cgtagctccc gaggccctgc
     1021 accgatgcgt cacctgtgcc ggcatcggag aggtggcgcc ccacgcccgg cctcctcggc
     1081 catcgctcaa gaccggcgcg acccatgccg aacctacccg tatgtaccga gggggaaccc
     1141 cattccgtcg tggtctggcg acgttcgccg cgatcgtctc catgggagcc acgctggtcg
     1201 ccggaggggt tacggcaacc gctgcaggcg gaggctcctc agccccccta ccgcacctgg
     1261 gggagcccgg atcgggcccg gcgccgttcc ccgtcgagct gccggcgacg gcatcgccgc
     1321 gagccgcgac cttccgatgg gccgacctcg acaactcgga cgcatgggcc aaggcagcca
     1381 tcgacttcgt cggcaaggcc aacgactgga tgcgcgactt cgccgcgaag ccggatggca
     1441 ccgtgccgtt caagcccgac atggtccaga cgcggaagta cctggcccgg gcagcggtca
     1501 aggcgttcgc acccgccgcc gacgtcgacc cggcgatcac gttcaccgac ctcgacccga
     1561 cgcaggcctt ctacaagtgg gcgaacatcg cggtgcagcg tggctggatg cgacgttccc
     1621 ccgacggtcg gttcatgccg gatcaggcgg tgacaacggg agtcctgcac tcggtcctga
     1681 tcgatgccct cggcatgtgg ggcatcgcgc ggcagctcga cgggctgcac acgcgggacg
     1741 gggcacattt ccagacaccc gaacggttcg gcgcgctcat gctcggactc cgactcgggc
     1801 tccgctacaa caacagcaac gaggcgatgg acgtcgggcc gaagagtcca ctcacccgcg
     1861 ctcaggtcgc ctactccctc tacaaggccg ccacccttcc ctcgtgggtg gtgccgtggg
     1921 tcagcgagca gtacgacggc atcgtgcttc ccaagatggg accgaagccg cgggcgatcg
     1981 tgcgctgggg cctgaaggcc gtcggtctcc cgtacgtgtg gggcggcgag tggggacggg
     2041 cgaacccacc ggcagccctc ggcggacagc cggtccccgg gttcgactgc tcgggcctgg
     2101 cctggtggct cctgcgagcc aacgacggcg gcgcatggga cgtgcacccg ccacgtccct
     2161 acgcgggctg ggccctcccc caacggacgt cggccgacat ggcccgcttc ggcgccctca
     2221 ggtacgaccg cctgctcccg ggggacctga tgttctacga cgggaacgac gacggaacgg
     2281 tcgaccacgt cgacgtctac atcggcaacg gctacgcgct cgattcgtcg aacacccctg
     2341 gcggggtcac catcatgtgg gtcggcgacg gctggtaccg cgagcacttc gtgcacggac
     2401 gccgcatcct accgtccgcc tgatcacgcc cccgcgccct cgatcgcccg ctgcaacacg
     2461 gcacgttcat cctcggtgag gggccggctg gaagggccgt ccggcgatct cggcacgatc
     2521 accgcctcgg cgtccgccgc gacggtgccg tcggccttct cgatgcgctc acgggtgcgg
     2581 atgctcgaac gcccgaaccc cgccacctcg cagtgcacga tcacgtcgcc gtcgtcctga
     2641 gtgagctggt tgcggaagtc gatcccgacg tgcgccagca cgaagtccca tgcctcgtcg
     2701 gcgccgaaga gcccttcgac catgcggtcc cggcactctt cgaggtagtt caggtacacg
     2761 gcgttgttca cgtgtccgta gttgtccatg tcgcgccagc ggatgtggac cgtcacctcg
     2821 accatgacgc ctatcctgcc aggcggtcga ggcatcggcc cgggccgccg gcctcccgac
     2881 atgggagaag gccccgggcg gggggcccga ggccttctct gtcggagaga ggggctccac
     2941 ccctacttcg gttcgcaccc gggcgtggtt cgcccccacc gtgttgcggt tgtgtgacgg
     3001 agggcggatc tagcgatcgc cctcggcgag ccgacggtcg gcctcctcgt cggcctgccg
     3061 gcggctgagc accgaccctg tgacggcggc gaggaacacg tcacgctcca gcgccaacag
     3121 gtcgacgtcc tcgagcgacg tgacggtcgc ggtcctcggc acgtcgcgca gcagcgcgat
     3181 ctcgccgaag tagtctcccg ggccgagttc ggcgacccgc accccgccct tcgtgacctc
     3241 gaccgtcccg tcgcgcacca cgtagacgcg gtcgccggtc tcaccctccc tgatcacgac
     3301 gccgcccgaa ggcacccgga acgggatcag gtgccaggtg agctgctcct gcacgtgctg
     3361 ggggagcggc gagaagatcg agacccggcg gagcaggtcg aatgccgggc cggggccgac
     3421 cgtgacctca tcgatccggc ggagacgtgg ccacgccccg agcgcgacga tcaccaggag
     3481 cgcgccagca gccacgagcg ctccggcagc gccgaaggct gagaccagca tcggggctgc
     3541 tgctgccccc accgccaggc cgatcatctg cagcgcctcc tccagaccga agacccgagc
     3601 gaggaccacg tcgtcggccc ggcgctgcag aagcgtgcgg ctggccaccg cgaacagccc
     3661 gatcgtggcc ccgcacaggg ccagcaccgc gaccgcgacc ggcatgctgg acgccaccga
     3721 gagcgcggcg agtgcggcgc ccgagccgag cagggcgacc agcaccgccg gcgcgagccg
     3781 ccgtaccccc accaggccga cggcccccgc cgctcccagc aacagaccca gaccgaaccc
     3841 gccggccaac acccccgcgc cgctctcgcc cgatcccagt acgtcgatcg cgagcagcgc
     3901 gaagaagacg tcgagcatcc cgaccatcac gaactgcacg ccccccaccc ccagcagtgt
     3961 ggcgacgtcg ggctcggcac gcgcctgccg gagcccgccg agggcctgcg acaggaaggt
     4021 gctcacgtgc tcggagggat gcggctcgtc cggtcgatcc gggcggaccc cgagcgctgc
     4081 ggccacggag agcgccgcga cgacggccat cgccaggacg gcgccgcccg gtccccagcg
     4141 agccatcagg accgccccgg ccagcggccc gatcagggtg ccgatcccat ccatcgtgct
     4201 cgacgcagcg ttggccgccg tcagctcgcc cggcgtcgtc gcgaggctgg gcaggatcgc
     4261 gttgtgcgcg ggtcgcgtga gtgtgatcac gctcgccgcc agcacggccg ccgcgtacgc
     4321 gaccggcgcg ggcgcctcga tgagacacac ccccaacacc aggttcgcgc aggcctggag
     4381 cccgtaaccc aggacgaggg ccttcgcccg cgggatgcgg tcggcgagca ccgatccgat
     4441 cggcgcgacg atcgccgccg gcacgagctg gagcaccagg accgcgcccg cctcacccgc
     4501 gcccccctgc tggaaggcgt agacggcgac ggcgacccat accgcgtact cctgcgtgtt
     4561 gaacagcagg aaggccacca gggcccgacg gagcgatcgg ttccggagtg cggtgcgcag
     4621 cacgccgaac gtggcggccg ctcgctccct caccttccgc gcctctccgt catcggttcg
     4681 accatccttg cggcgcagtc gagagcggtc aaaccccgat gacccaggaa acgcggaacg
     4741 gggccccgaa gggccccgtt cggtgtcgtg cgaccgcgtt ggactcagaa gtccatgtcg
     4801 ccgccgggca tgccgccggc cggcatggcc ggcgccttct ccggcttctc ggcgacgatg
     4861 gcctctgtcg tgaggaacag cgcggcgatc gacgcggcgt tctgcagcgc cgagcgcgtc
     4921 accttggtgg ggtcgatcac gccgaacttc agcatgtcgc cgtactcacc gttggccgcg
     4981 ttcagcccat accccgggtc gagcgctcgc accttctcga cgatcacgcc gccctcgagg
     5041 ccggcgttca cggcgatctg cttcagcggc tcctcgagcg cacgacggac gatgcttgcc
     5101 cccgtcgcct catctccctc gaggtcgacc ttgtcgagcg cggcctgcgc gttcagcaac
     5161 gcgacaccgc caccgggcac gatgccctct tcgaccgcag ccttggtcga ctgcaccgcg
     5221 tcctcgatgc ggtgcttctt ctccttgagc tcgacctcgg tggccgcgcc gaccttgatg
     5281 accgccacgc ccccggagag cttggccagc cgctcctgca gcttctcgcg gtcgtagtcc
     5341 gagtcggtct tctcgatctc acccttgatc tggttgatgc ggccttggac gtcctcgtcc
     5401 ttgcccgcgc cttccacgat cgtcgtctcg tccttggtca cgatgacctt gcgagcggtg
     5461 cccagcaggt ccagcgtggc gttctcgagc ttcaggccga cctcctcgga gatgaccgtg
     5521 gcgcccgtga ggaccgcgat gtcctgcagc atggccttgc gacggtcccc gaatccggga
     5581 gccttcaccg aggcgctacg gaacgtgcca cggatcttgt tgaccacgag ggtcgcgagg
     5641 gcttcaccct cgacgtcctc ggccagcacc agcacgggct tgcccgcctg catgaccttc
     5701 tcgagcaccg gcagcaggtc cttcacggcc gagatcttcg agttcgcgat caggatgtac
     5761 gggtcctcga gcacggcttc catgcgctcg gagtcggtga cgaagtacgg cgagatgtag
     5821 cccttgtcga agcgcatgcc ctcgacgaag tcgagctcca tgccgaacgt gttggactcc
     5881 tcgaccgtga tgacgccgtc cttgccgacc ttgtcgacgg cctcggcgat cgtctcgccg
     5941 atctgggggt cggccgcgga gatcgcgccg acgttggcga tctgggcctt gtcgtcgacc
     6001 tccttggact ggttcttgat cgcctcgacg gcgagctcgg tcgcacgctc gatgccgcgc
     6061 ttcaggctca tcgggttggc gcctgccgcg acgttgcgca ggccttcctt cacgatcgcc
     6121 tgagcgagca cggtggccgt cgtcgtgccg tcaccggcga cgtcgtcggt cttcttcgcg
     6181 acctccttga ccagctcggc accgatcttc tcctcggggt cgtcgagctc gatctccttg
     6241 gcgatggaga cgccgtcgtt cgtgatcgtg ggggcgcccc acttcttctc gaggacgacg
     6301 ttgcgacccc tggggccgag cgtgatcttc acggcgttgg cgagctggtc catgccccgc
     6361 tcgagcgcgc gccgtgcgtc ctcatcgaaa gagatgatct tcgctgccat cccttctcct
     6421 ttcctggccc gtgggctcgt tggtggtcgg tcggttctcc ggctggcttg cagactgcta
     6481 gcactcgggg gtgccgagtg ctaacgattg tagccgcaaa ccgggtgtgg gtggcaagcc
     6541 ttcctcgcgc gcggctccgt cgcctgtgtc gggtatccga cacggctccg cgcggtttga
     6601 gcggtaggct cgcgcgtcgt gaccccgcct cgaacgggag gagcctgacg tggtcatcag
     6661 tctcgattgg cggcaccgcc tcaccgctcc cacgatcggc ggcgtcggct ggaactcggc
     6721 ccgcccgggc cgtctcgcgg tcgtctcgac ggagagcggc acctcgcagg cgtg
//
LOCUS       DATDBZ010000193         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1066247, whole genome shotgun sequence.
ACCESSION   DATDBZ010000193 DATDBZ010000000
VERSION     DATDBZ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066247"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VIX62_04755"
     CDS             complement(<1..583)
                     /locus_tag="VIX62_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018549021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5859574.1"
                     /translation="MSDQPLAARMRPRTFEEYEGQAHLVGEGTALSRLLAGGNLPSII
                     LWGPAGTGKTTLAHLLANAVGADLIQLSAVSSGVADARKVMQGAKGGMFRTVLFVDEV
                     HRWSKAQQDVLLPAVEEGIVTLIGATTENPYFSLNTPLLSRCLLLRLDPLGDEELATL
                     LRRALADEDRGLGRHHLTIADDALAHLVTVSGGD"
     gene            complement(612..1001)
                     /locus_tag="VIX62_04760"
     CDS             complement(612..1001)
                     /locus_tag="VIX62_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEY5859575.1"
                     /translation="MAELTPEQLAVFGPPNLAYLATINEDGSPHLSPLWADTRDGMIV
                     LNTADGRVKVENVRRDPRVSVAIHDKEHPHPPIAVVGTVVDITTEGAAEHMQELALAY
                     DGEAWTPVEGQVRLLLVIRPDHVLGWT"
     gene            complement(994..>2089)
                     /locus_tag="VIX62_04765"
     CDS             complement(994..>2089)
                     /locus_tag="VIX62_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEY5859576.1"
                     /translation="TQLDVEMSFVTEEDLIALIEPLYARIVQDIAGVEVAAPFPRMTY
                     AEMMQRYGSDKPDLRYGMELEDLGPVFARTGFNAFAKVLADGGAIKALAAPGAGSFSR
                     KELDKLIEEAKGRGAAGLVWLVVEADGAVRSPVEKFLSPEEIAGIVAATGAAEGDLVC
                     IVADRTDRANVALDGLRRDLAVRLDLIPENAWTFCWMVEPPLFEWSDEEGRWVSVHHP
                     FTSPAGDDLTPETAKARAYDLVLNGFEIGGGSIRIHRADVQRRIFENLGLTAEQIDDE
                     FGHLIGALRLGAPPHGGIAMGVDRLVMIMAGKDAIRDVIAFPKSQSGGDPLTGAPAPV
                     DEDQLRELGIRLAHPPKAAEPATTKEDVDG"
BASE COUNT          303 a          730 c          724 g          332 t
ORIGIN      
        1 cgtcgccgcc ggacacggtc accaggtgtg cgagggcgtc atcggcgatc gtgaggtggt
       61 gacggccgag gcctcggtcc tcatcggcga gcgcgcggcg cagcaaggtg gcgagctcct
      121 cgtcgccgag cgggtcgagc cgcagcagga gacagcgcga gagcagcggg gtgttcaacg
      181 agaagtaggg gttctcggtg gtggcgccga tcagcgtgac gatgccctct tccaccgccg
      241 gaagcagcac gtcctgctgc gccttcgacc agcggtgcac ctcgtcgacg aacagcaccg
      301 tccggaacat cccgcccttg gcgccctgca tcaccttgcg cgcatcggcc acgccggacg
      361 agaccgccga gagctggatc agatcggcgc ccaccgcgtt ggcgagcaga tgcgccaggg
      421 tggtcttgcc ggtgcccgcc ggcccccaca ggatgatcga cgggaggttg ccgccggcga
      481 gcaatcgcga cagcgcggtg ccctcgccca ccaggtgggc ctgcccttcg tactcctcga
      541 acgtccgagg gcgcatgcgc gcggcgagcg gttgatcgct cacgggcgtt cgtgcatccc
      601 caacgcgctc cctacgtcca accgaggacg tggtcgggcc tgatcaccag caggaggcgc
      661 acctgtccct cgaccggggt ccacgcctcc ccgtcgtagg cgagggcgag ttcctgcatg
      721 tgctcggcgg caccctccgt ggtgatgtcg acgacggtgc cgaccacggc gatcggtgga
      781 tgcggatgct ccttgtcgtg gatcgccacc gacacgcgcg gatcgcgccg cacgttctcg
      841 accttcacgc ggccgtcggc ggtgttgagc acgatcatgc cgtcgcgggt gtcggcccac
      901 agcggcgaca ggtgcggtga accgtcctcg ttgatcgtgg cgaggtaggc caggttgggc
      961 ggaccgaaga ccgcgagttg ttcgggcgtg agctcagcca tcgacatcct ccttggtcgt
     1021 ggcgggttcg gcggccttcg gcgggtgcgc cagacggatg ccgagctcgc gaagttggtc
     1081 ctcgtccacc ggggcgggag cgccggtgag cgggtcgccg cccgactggg acttcgggaa
     1141 cgcgatgacg tcgcggatcg cgtccttgcc cgccatgatc atcacgaggc ggtcgacccc
     1201 catcgcgatc ccgccgtgcg gcggcgcgcc gagccggagc gccccgatca ggtgaccgaa
     1261 ctcgtcgtcg atctgttcgg cggtgagtcc gaggttctcg aagatccgtc gttggacgtc
     1321 ggcccggtgg atccggatgc tgccgccgcc gatctcgaat ccgttcagca ccaggtcgta
     1381 ggcgcgagcc ttcgcggtct cgggcgtcag gtcatcgccc gccggggagg tgaatgggtg
     1441 gtgcaccgac acccagcggc cctcctcgtc gctccattcg aacagcggcg gctcgaccat
     1501 ccagcagaac gtccaggcgt tctcggggat gaggtcgagt ctgacggcaa ggtcgcgccg
     1561 aaggccgtcg agggccacgt tggcccggtc ggtgcggtcg gccacgatgc acaccaggtc
     1621 gccttcggcg gccccggtcg cggccacgat gccggcgatc tcttcagggc tcaggaactt
     1681 ctccaccggc gaccgcaccg cgccgtcggc ttcgacgacg agccacacga ggccggcggc
     1741 gccacggccc ttggcctctt cgatcagctt gtcgagctcc ttgcgcgaga acgaccccgc
     1801 gcccggcgcc gccagggcct tgatcgcacc cccgtcggcc agcaccttcg cgaacgcgtt
     1861 gaaccccgtc cgggcgaaca ccggcccgag atcctccagc tccataccgt atcggaggtc
     1921 aggcttgtcc gatccgtacc gctgcatcat ctcggcgtag gtcatgcgcg ggaacggcgc
     1981 ggccacctcg acccccgcga tgtcctgcac gatccgcgcg tacagcggct cgatcagggc
     2041 gatcaggtcc tcttcggtga cgaagctcat ctccacgtcg agctgggtg
//
LOCUS       DATDBZ010000194         7466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1497397, whole genome shotgun sequence.
ACCESSION   DATDBZ010000194 DATDBZ010000000
VERSION     DATDBZ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497397"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VIX62_04770"
     CDS             <1..517
                     /locus_tag="VIX62_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY5859577.1"
                     /translation="DTLEPSWRGEYEITEAIQWLVDHGHTVRAEMVEGWWKDTGKPDD
                     LLEANRMMLSVRGHAIDGDVDEATSVEGSVVVAAGAKVTRSVLRGPVVIGPDSVVDES
                     TVGPDVSVERGCRIERSEVADSIVMEGCTIADVRGIAGSILGRGVEVRHSGTEGIHRL
                     IVGDQSRVEVD"
     gene            616..996
                     /locus_tag="VIX62_04775"
     CDS             616..996
                     /locus_tag="VIX62_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859578.1"
                     /translation="MDHIDPQPPRVPGRRMAFQAFVLAAVGPFLMGASSPAAIVLGFR
                     SLSLSSVAGARAPGRATAYGAIVLGACGLWLWGWTVARLAGTLSARGKDPALVMPLIA
                     GLLFVWLASGLAGFLAGRAARDLG"
     gene            1009..2166
                     /locus_tag="VIX62_04780"
     CDS             1009..2166
                     /locus_tag="VIX62_04780"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5859579.1"
                     /translation="MRAVIDARPALDPRRTGVGLYTQQIIRHLPHADPDNEYVAWYLH
                     AKGLFRPRRFFEGVNAPNLTEKASRFPARVFGPMSTRLGVPRIEWSGAFDVLLATNFL
                     PPATHSPGVVMMVHDLAHEHLPETAPHMDHRWRRRFDRWLQRAAGVIVPSASTKADLL
                     AAHDVDADRVHVTPLGVDVAAFAPAAPDAIEAVRRKYGIDGAYALFLGGIEPRKNLDL
                     LVQAFASLEPGTRLVIAGGSVRWDPKAIDRLGEVIGALPPAARARIVRTGYVADREKI
                     ALLSGATLLAYPSRYEGFGFPVLEGFAAGVPVLTSDVSSLPEVAGDAAVLVDPRDPDA
                     IADGLRQLFDDPDLRAMLSAAGLARAAKFTWEATARGTAAALHAAHEVARG"
     gene            2201..3187
                     /locus_tag="VIX62_04785"
     CDS             2201..3187
                     /locus_tag="VIX62_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019855406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY5859580.1"
                     /translation="MANDPSNEHVLVTGGAGFIGSHLVDLLLEHPDARVTVLDKLTYA
                     GSKANLAQHDGDDRLRFVQGDVADPSVVAPLVADVSRVIHAAAETHVDRSISGPAEFV
                     LTNVVGTQVVLDACRTYDRPLLLVSTDEVYGQNEPEGTFDEGSPIRPRSPYAASKAAA
                     ELLARSYGITYGLPVWIVRGTNAFGPRQNPEKAIPVFTTAALEGRPLPVYDDGRQSRE
                     WLYVTDWAAACITALYHGEPGDVFNIGDGFELANADLARSICRLVGADESLVTFVPDR
                     PGHDLRYGMASERLHELGWRPAVPFDEGLARTVEWYRVHPDWVREMRAGAGA"
     gene            3184..4026
                     /gene="rfbD"
                     /locus_tag="VIX62_04790"
     CDS             3184..4026
                     /gene="rfbD"
                     /locus_tag="VIX62_04790"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY5859581.1"
                     /translation="MRLVVTGAGGGLGRAFLAQMPAHHEVQAFDHAALNIGDHDAVRQ
                     TIDPLRPDAIVNLAAFTKVDANEAEPARAFRDNAMGPQHLALSARATGAVLLHISTDY
                     VFDGAKDAPYDETDEPAPLSVYGRAKLEGERFVRQIAPASFIVRVGYVFGGGDDYLTG
                     AARRLAAGEDAGGLRDRVGSPTFVGHVAERLLPLALTGRFGTYHLAGPQAASWFEVLQ
                     RVRDLGGFPGAVVPQTAAELALPAPRPAYSALTSVYLEHLGVPPMPSLDEGLKTFLGS
                     LDRS"
     gene            4023..5375
                     /locus_tag="VIX62_04795"
     CDS             4023..5375
                     /locus_tag="VIX62_04795"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5859582.1"
                     /translation="MRPGRVRITSVGLLVVAGMFATACSSADPADESPSQSPRPTAAS
                     PVVTTPSAAAVVDANAFPTRWPIKHVVFLIKENRTFDNLFGTFPGARGVSFGFDRGVR
                     RPLTRGTDQATHEDIPHCYECALAAWNHGEMDGFNQSEPADRWAYTQLHKDQLPNYWE
                     WASHNALFDNFFASAQGPSFPNHLYSIAAQSGGAHDNPRRKPGLGSLTFGCDAPDEQL
                     VEVVDSEGVTKMVKPCFDFLTEGDLLNRAKIPWAYYAAQEDQKGYIWSAYSAIRRYRE
                     DPKRWRQHIFPVDRVVKDIRENTLPPVTWITPKFELSEHPEYNFCHGENWSTKVINAI
                     MQSDHWKDTAIFLTWDDYGGFYDHVPPPQVDDFGFGIRVPMIVLSPYAVDGEVSHELG
                     EFSSVLRFIEDNWGLTKLTHRDREATPMMSAFDFTQEPRAPQPLPMRDDCKGPVYFEP
                     "
     gene            5372..5920
                     /locus_tag="VIX62_04800"
     CDS             5372..5920
                     /locus_tag="VIX62_04800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5859583.1"
                     /translation="MIEPTIRLIRTDDLRPEEVASLRALFDAAWSNKDGRFSEDDWTS
                     ASGGTHVIASIGDRIVSHASVVERVLETGDLVLRTGYVEAVATWPEHQGAGHATRVMR
                     AVGELLGGYGLGALDTGTPAFYERLGWERWRGPTAVRTERGVIRTPEEDGLVMVLRTP
                     STPEGLDLEAAISCDWRPGDVW"
     gene            5972..6400
                     /locus_tag="VIX62_04805"
     CDS             5972..6400
                     /locus_tag="VIX62_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859584.1"
                     /translation="MTADPTSMAPGEDPQDDLLREMYWARETAARGRMDTSEVRDRLH
                     DLAAQLRTDETMVIPMGEGNLASPSRWRRQIKYRMWRVMRPATWRYDRLIADHAELTT
                     SLAEKVMVLEAEVDRLKSELDAERSGNATGNDRPIEEPPA"
     gene            6397..7446
                     /locus_tag="VIX62_04810"
     CDS             6397..7446
                     /locus_tag="VIX62_04810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5859585.1"
                     /translation="MRVAVLHPQTAFVRGGAETHAESLVKALKAAGHDADLVQIAGKW
                     YPPSQLAHQMAVWRSFDITESNGLKVDAVIGLKFPAYLAQHERKVVWLIHQHRTAYEL
                     WDHADYADLKRQDDGAAVRDMVWEADRVALGEAKRVFTNSSNVKDRLWNSLRIPAETL
                     YHPSGVSERLLDREPGPYGEHILFPSRMESLKRQSLVIDAMKHVKTSVQLVLVGRGPD
                     ESQLREQVERLGVGDRVTFEIGVSDERLEQLYLSALGVYFGPFDEDYGYVTLEGMAAE
                     RPVVTLTDSGGPLEFVRDDETGIVAPPEPRAIAEAFDRLHRDREAAARLGKAGNALIR
                     AEVPRWPDVVARLLD"
BASE COUNT         1125 a         2589 c         2600 g         1152 t
ORIGIN      
        1 cgacaccctg gaaccctcgt ggcgcggtga gtacgagatc accgaggcga tccagtggct
       61 ggtcgaccac gggcacacgg tccgtgccga gatggtcgaa ggctggtgga aggacaccgg
      121 caagcccgac gacctgctcg aagccaaccg gatgatgctg tcggtgcgtg gccacgcgat
      181 cgatggcgac gtcgacgaag ccacctcggt ggagggctcg gtcgtggtgg ccgccggagc
      241 caaggtcacc cgcagcgtgt tgcgcgggcc cgtcgtgatc ggtccggact cggtggtgga
      301 cgagtcgacc gtgggtcccg atgtctcggt cgagcggggc tgccggatcg aacgcagcga
      361 ggtcgcggac tcgatcgtga tggaggggtg cacgatcgcg gacgttcgag gcatcgccgg
      421 ctcgatcctg ggccggggtg tcgaggttcg tcattccggc accgagggca tccacagact
      481 gatcgtgggg gatcagagcc gcgtcgaggt cgactgaccg gcccggaccg accggggccg
      541 aacggtcttc ggcggcatcg gacctgaggc cgaagacggt tccttcggcg ttcgccgcgt
      601 cctaccatcg cgcagatgga ccacatcgat ccccagcctc cccgcgtgcc cggccgccgc
      661 atggcgttcc aggcgttcgt gctcgcggca gtgggaccgt tcctgatggg cgctagctcg
      721 ccggccgcga tcgtgctggg gttccgctcg ctcagcctct cgtcggtcgc cggcgcccga
      781 gctccgggcc gcgccaccgc gtacggggcg atcgtgctcg gcgcgtgcgg cctgtggctg
      841 tgggggtgga ccgtggcgcg gctcgcgggc acgttgtccg cccgcggcaa ggaccccgcc
      901 ctggtcatgc cgctgatcgc ggggctgctc ttcgtgtggc tggcctcagg gctggccggg
      961 ttcctcgcgg ggcgtgcggc acgcgacctc gggtagggtt cggggttcat gcgagccgtg
     1021 atcgacgccc ggccggcgct cgatccccga aggacggggg tggggctcta cacgcagcag
     1081 atcatccgtc atctgccgca cgccgacccc gacaacgagt acgtggcgtg gtacctgcac
     1141 gccaagggcc tgttccggcc gcgccggttc ttcgaaggcg tgaacgcccc caacctcacg
     1201 gagaaggcct cccggttccc ggcgcgcgtg ttcggcccca tgtcgacccg cctcggcgtg
     1261 ccgcggatcg aatggtcggg cgcgttcgac gtgctgctgg cgacgaactt ccttcccccg
     1321 gccacccaca gccccggggt ggtgatgatg gtgcacgacc tcgcgcacga gcatctgccc
     1381 gaaacggccc cccacatgga tcatcggtgg cgccgccggt tcgaccgctg gctgcagcgg
     1441 gcggcggggg tcatcgtgcc gtcggcctcc acgaaggccg acctgctggc cgcgcacgac
     1501 gtcgatgccg atcgtgtcca cgtcacgccg ctcggggtcg acgtcgcggc cttcgcgccg
     1561 gcggcgcccg atgcgatcga ggcggtccgg cgcaagtacg gcatcgatgg cgcctacgcc
     1621 ctgttcctcg gcgggatcga gccacggaag aacctcgacc tgctcgtgca ggcgttcgca
     1681 tccttggagc ccggcacgcg cctggtgatc gcggggggct cggtccgatg ggaccccaaa
     1741 gcgatcgacc gcctgggtga ggtgatcggg gcgttgccgc ctgcggctcg cgcccggatc
     1801 gtgcggacgg gctacgtggc cgaccgggag aagatcgcgc tcctcagtgg cgccacgctc
     1861 ctggcctacc cgtcacggta cgaaggcttc gggttcccgg tgctcgaagg gttcgctgcg
     1921 ggggtgccgg tgctcacgtc ggacgtgtcg tcgttgcccg aggtcgccgg cgacgccgcc
     1981 gtgctggtcg atccgcgtga ccccgacgcg atcgccgatg gcctgcgcca actcttcgac
     2041 gatccggacc tgcgcgcgat gctgtccgcg gccggactcg cgcgtgccgc gaagttcacg
     2101 tgggaggcca cggcacgcgg caccgctgct gccctgcatg ccgcccacga agttgcccgc
     2161 gggtagactc gcaccgtccc tgcaccggcg aggttccgct gtggccaacg acccctccaa
     2221 cgagcacgta ctggtgaccg gcggtgccgg gttcatcggc tcgcacctgg tcgacctgct
     2281 gctcgagcac cccgacgctc gtgtcacggt cctggacaag ctcacctacg cgggatcgaa
     2341 ggccaacctc gcccagcacg acggcgatga ccgcctccgg ttcgtgcagg gggacgtcgc
     2401 cgatccctcc gtggtcgccc cactcgtggc cgacgtctcc cgcgtgatcc acgccgcggc
     2461 cgagacgcac gtcgaccgtt cgatcagcgg tcccgccgag ttcgtgttga ccaacgtcgt
     2521 cggcacgcag gtggtgctcg acgcctgccg gacctacgac cggccgctcc tgctggtctc
     2581 gaccgacgag gtctacggcc agaacgaacc cgagggcacc ttcgacgagg gatcgccgat
     2641 ccgtccgcgc agcccgtacg ccgcgagcaa ggcggcggcc gaactcctgg ctcgctccta
     2701 tggcatcacc tacggcttgc cggtgtggat cgtgcgaggc accaacgcgt tcgggccccg
     2761 gcagaacccg gagaaggcga tcccggtctt caccaccgcc gccctcgaag gccggccgct
     2821 ccccgtctac gacgacggcc gccagtcgcg cgaatggctg tacgtcaccg actgggccgc
     2881 cgcctgcatc accgcgctgt atcacggcga acccggcgat gtcttcaaca tcggcgacgg
     2941 cttcgaactg gcgaacgcgg acctggctcg ttcgatctgc cgcctggtgg gtgcggacga
     3001 gtcgctggtg acgttcgtgc ccgaccgccc cggccacgac ctccgctacg gcatggcgag
     3061 cgagcgtctg cacgaactcg gttggcgtcc cgcggtgccg ttcgacgaag gccttgcgcg
     3121 aaccgtcgag tggtaccggg tccatcccga ctgggttcgc gagatgcggg cgggggccgg
     3181 cgcatgaggc tggtcgtcac cggggcgggt ggaggcctgg gccgggcgtt cctcgcgcag
     3241 atgccggcgc accacgaggt gcaggcgttc gaccacgcgg cgctcaacat cggtgatcac
     3301 gatgcggtgc gccagaccat cgacccgctc cggcccgatg cgatcgtgaa cctcgccgcg
     3361 ttcaccaagg tcgacgccaa cgaggccgag cccgcgcgcg cgttccgcga caacgcgatg
     3421 ggcccgcagc acctcgcgct gtcggcgcgc gccaccggcg cggtgctgct ccacatctcc
     3481 accgactatg tgttcgacgg ggcgaaggat gcgccctacg acgagaccga cgaaccggcg
     3541 ccgctgtccg tttacggccg agccaagctc gaaggcgagc ggttcgtccg ccagatcgcg
     3601 ccggcgtcgt tcatcgttcg ggtgggctac gtgttcggcg gtggcgacga ctacctcacg
     3661 ggtgccgctc ggcggctcgc tgccggggag gacgccggcg gtctgcgaga tcgcgtcggg
     3721 tcgccgacgt tcgtgggtca tgtcgccgag cggctgctcc cgttggcgct cacggggcgg
     3781 ttcggtacct accacctcgc cggaccccag gctgcctcgt ggttcgaggt gctccaacgg
     3841 gtgcgtgatc tcggcgggtt ccccggcgcc gtggtgccgc agaccgcggc cgaactcgca
     3901 ctcccggcac cacgcccggc gtattcggcg ctgaccagtg tctacctgga gcacctcggt
     3961 gtaccgccga tgccgtcgct cgacgaaggg ctcaagacgt tcctcggtag cctcgatcgc
     4021 tcatgaggcc gggccgggtt cgtatcacct ccgtcgggct gctcgtggtt gccgggatgt
     4081 tcgcgaccgc atgttcgagc gcggaccctg cggacgagtc gccgtcgcag tcgccgcgac
     4141 cgaccgccgc gtcgccggtg gtgaccacac cgtcggcagc cgcggtcgtc gatgcgaacg
     4201 cgttcccgac gcgctggccg atcaagcacg tggtgttcct gatcaaggag aaccggacct
     4261 tcgacaacct gttcggcacg ttcccgggtg cgcgcggcgt ctcgttcggc ttcgaccgcg
     4321 gggttcggcg gccgctgacc cgcggcaccg accaggcgac gcacgaggac atcccgcact
     4381 gctacgagtg tgcgctggcg gcgtggaacc acggcgagat ggacgggttc aaccagagcg
     4441 aaccggcgga tcgttgggcc tacacgcagc tccacaagga ccagctgccc aactactggg
     4501 agtgggcctc gcacaacgcg ctgttcgaca acttcttcgc cagcgcgcag ggaccgtcgt
     4561 tcccgaacca tctgtactcg atcgcggcgc agtccggcgg cgcgcacgac aatccgcgtc
     4621 gcaagcccgg tctcgggtcc ctcacgttcg ggtgcgacgc gcccgacgaa cagctggtgg
     4681 aggtcgtcga ctccgaaggg gtcacgaaga tggtgaagcc gtgcttcgac ttcctgacgg
     4741 agggcgacct gctgaaccgc gcgaagatcc cgtgggccta ctacgcggcc caggaggatc
     4801 agaagggcta catctggtcg gcgtacagtg cgatccgccg ctaccgcgag gaccccaagc
     4861 gctggcggca gcacatcttc ccggtggacc gtgtcgtgaa ggacatccgc gagaacacgc
     4921 ttccgcccgt cacgtggatc acgcccaagt tcgagctcag cgagcatccc gaatacaact
     4981 tctgccacgg cgagaactgg tccaccaagg tgatcaacgc gatcatgcag agcgatcact
     5041 ggaaggacac cgcgatcttc ctcacctggg acgactacgg cggcttctac gaccacgtgc
     5101 cgcccccgca ggtggatgac ttcgggttcg ggatccgcgt gcccatgatc gtgctcagcc
     5161 cgtatgcggt ggacggcgag gtctcgcacg agctcgggga gttctccagc gtgctgcggt
     5221 tcatcgaaga caactggggc ctcacgaagc tcacccatcg cgaccgggaa gccacgccga
     5281 tgatgtcggc gttcgacttc acgcaggaac ctcgcgcacc ccaaccgttg ccgatgcggg
     5341 acgactgcaa aggtcccgtg tacttcgagc cgtgatcgaa cccacgatcc gcctgatccg
     5401 aacggacgac ctgcgtcccg aagaggtcgc ctcgctgcgc gcgctcttcg acgccgcctg
     5461 gtcgaacaag gacgggcgat tctccgagga cgactggacc agcgcgtctg gtggcacgca
     5521 tgtgatcgca tcgatcggcg accggatcgt gtcgcacgcc tcggtcgtcg agcgcgtcct
     5581 ggagaccggc gacctcgtcc tgcgaaccgg ctacgtcgaa gcggtcgcca catggcccga
     5641 acaccaaggc gccggtcatg ccacgagggt gatgcgcgcg gtgggcgagt tgctcggcgg
     5701 ctacggattg ggtgccctcg acaccggcac ccctgcgttc tacgaacgtc ttgggtggga
     5761 acgctggcgc ggaccgaccg ccgtccgcac cgaacggggc gtcatccgca cgccggagga
     5821 ggacggcctc gtgatggtgc tcaggacgcc ctcgacgccg gaaggcctcg atctcgaggc
     5881 cgcgatcagc tgcgactggc gccccgggga cgtctggtga cctgcgggaa cgccacggag
     5941 ccgtgacagg cgggtgctag cctcgaccgc gatgacggcc gaccccactt ccatggctcc
     6001 cggcgaggat ccgcaggacg atctgctgcg cgagatgtac tgggcgcggg agaccgcggc
     6061 gcgcggccgt atggacacct ccgaggtgcg tgaccgcctg cacgatctcg ccgcgcagct
     6121 gcgcaccgac gagacgatgg tgatcccgat gggggagggc aacctcgcct cgccgtcgcg
     6181 gtggcgtcgc cagatcaagt accgcatgtg gcgggtgatg cgtcctgcca cctggcggta
     6241 cgaccgcttg atcgccgacc acgccgagct caccaccagt ctcgccgaga aggtcatggt
     6301 cctcgaggcc gaggtcgatc gattgaagag cgaactcgac gcggagcgct ccgggaacgc
     6361 caccgggaac gaccgcccga tcgaggagcc gccggcgtga gggtcgccgt cctccatccc
     6421 cagaccgcgt tcgtgcgcgg cggcgccgag acccacgccg agtcgctggt gaaggcgctg
     6481 aaggccgcgg gccacgacgc cgacctggtg cagatcgcgg gcaagtggta cccgcccagc
     6541 cagctggcgc atcagatggc ggtgtggcgc agtttcgaca tcaccgagtc gaacggcctc
     6601 aaggtcgacg ccgtgatcgg cctgaagttc cccgcctacc tcgcgcagca cgagcgcaag
     6661 gtggtgtggc tgatccacca gcaccgcacc gcgtacgagc tgtgggatca cgccgactac
     6721 gccgatctga agcgtcagga cgacggcgcc gcggtgcggg acatggtgtg ggaggccgac
     6781 cgcgtggccc tcggcgaggc caagcgcgtg ttcaccaact cctcgaacgt gaaggaccgc
     6841 ctttggaact cgctgcggat cccggccgag acgctgtatc acccttcggg cgtgagcgaa
     6901 cggctcctcg atcgtgaacc cggcccctac ggcgagcaca tcctgttccc cagccgcatg
     6961 gagtcgctga agcggcagtc actggtgatc gacgccatga aacacgtgaa gacgtcggtc
     7021 cagctcgtgc tcgtcggccg tgggccggac gaatcccagc tccgggaaca ggtcgagcgc
     7081 ctgggggtcg gtgatcgcgt caccttcgag atcggggtca gcgacgaacg tctcgaacag
     7141 ctctacctgt ccgcgctcgg cgtgtacttc ggccccttcg acgaggatta cggctacgtc
     7201 acgctggaag gcatggcggc ggaacgaccg gtcgtcacgc tcaccgacag cgggggaccg
     7261 ctcgagttcg tgcgggacga cgagaccggg atcgtcgccc cgccggagcc gcgggcgatc
     7321 gccgaggcct tcgaccggct gcaccgtgac cgggaggcgg ccgcccggtt gggcaaggcc
     7381 ggcaacgcgt tgatccgggc cgaggtgccg cgctggccag acgtcgtcgc gcggctcctg
     7441 gactgatggg cgccagggcg aaggcc
//
LOCUS       DATDBZ010000195        12870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2309600, whole genome shotgun sequence.
ACCESSION   DATDBZ010000195 DATDBZ010000000
VERSION     DATDBZ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12870
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309600"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VIX62_04815"
     CDS             <1..400
                     /locus_tag="VIX62_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY5859586.1"
                     /translation="AHTYFVPVSHTERARSVLGPEPVLAVELTAIHATDPTRARHLAR
                     AWARHYLELPNYAKNLVRMGFSEDEVTGDGSDRLIDATIAWGDAGAVAARVREHLDAG
                     ADHVCVQYIDADDADLCLPAYAELAESVLA"
     gene            complement(404..1102)
                     /locus_tag="VIX62_04820"
     CDS             complement(404..1102)
                     /locus_tag="VIX62_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEY5859587.1"
                     /translation="MKAASRQVVDELRSVADPSRRPGMARVGITIDRALGVSIPNCRR
                     LARRHRGDHALALDLWTTKIHEARILASMVDDPSQVTVEQMEAWATDFDSWDLCDQVC
                     GNLFTETPHSFDRARAWVRREEEFVKRAGFSIVAEHAARARDDHDDAFWLGWLLEIER
                     GAVDERNYVKKAVNWALRQIGKRDLALNAAAIDTGERLAASPSRSARWIARDALRELR
                     SDGVQARLRARASR"
     gene            complement(1099..1500)
                     /locus_tag="VIX62_04825"
     CDS             complement(1099..1500)
                     /locus_tag="VIX62_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEY5859588.1"
                     /translation="MDRAIVLYDEDCGVCRWTADRLRRWDRRDRLTFRSIQTAEAGGD
                     LDAMDPAARYASWHVVEPDGRIWSAGAAIPRVMRRLPGGRPVATLTATFPAATERMYG
                     FVVRHRTRLGRMLGQRACSVDPSAITGAREP"
     gene            complement(1543..2613)
                     /locus_tag="VIX62_04830"
     CDS             complement(1543..2613)
                     /locus_tag="VIX62_04830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5859589.1"
                     /translation="MRSSLPHRRSSLVLIAVVLSALPMCGGSTPTPPPPIRITVDGTT
                     HAFAPGTTFAGVIAELGLSAADGRLLSVSGEVLDPLARRGHIVLNGTPAAPGTRLRQG
                     DAIQVLDRHDRVEGTRRVVVDIKEPQVANPERTLTRYPIREITIEGRISGDVVSVREN
                     PRGHGETPGAVALTFDDGPWPVHTERVLSILHRFHVKATFFMVGYEVERYPELVRRVK
                     RAGHEIGNHSFDHPENPPLADMTEAHITAEMADTNAQLGEVGVHPTLFRPPGGSYDDF
                     VIDEARRQGMRIVMWSVDPQDWRSSRTRKDIVKKVLKQVEAGSIILLHDGGGDAGHTI
                     AALPDLIRGIRKKGLRFVTVPA"
     gene            2684..3145
                     /locus_tag="VIX62_04835"
     CDS             2684..3145
                     /locus_tag="VIX62_04835"
                     /inference="COORDINATES: protein motif:HMM:NF022700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HEY5859590.1"
                     /translation="MSTRKVKRPRAGRHPNQDPAPPPPRPETPAAWLIAADTVALVVF
                     VLAGMRSHHEGSAAWIFGRNAIPLLIAWFAVAALLKTYRTPGFMIVAKTWIVAVPIGL
                     TVRSLWVGSPTGWRFVVFLAVGLGFTMLFLLIGRLLLALITGRGYPQRRRT"
     gene            3142..4596
                     /locus_tag="VIX62_04840"
     CDS             3142..4596
                     /locus_tag="VIX62_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859591.1"
                     /translation="MSTRLSMPDPGAARSFWLQEALGADPGEPCPPLEAHVVADVCIV
                     GGGFAGLWTAIELSAREPTMRIALLEQDICGGGASGRNGGFFSSSWWDVEALCGLFGD
                     DDGLRYAHAVSDTVGEAGTWLADHDVDAWFHHDGVLGIRTGTWQEGPGGEGPAAFLEA
                     RGLGDRMRPLTVEQAREIADSPRITGARFIDDSAIVQPARLARGLRRIALERGVRIFE
                     RTTVTGLDRSRPAVVRTEHGAVKADQVVLAIGAWAAGWPGFRRSFGVIADHMVVTEPI
                     PERLAEIGWTSFAGIADGRDLLFYLRPTDDHRIAIGGGGLAVVFGGRASGRSATHDRR
                     VADVAARGLLWLFPQLEGVRFTHAWGGPIDQTPSFLPFYRTLQPGNVHAGLGFSGHGL
                     SQTMVGSRILASMVLGVEDRWTSLPVVGGELGKAPPEPFRYPAIKAAGWALERGDRRA
                     DAGRPRGWLDATVGAAPQAWRDRLVARASRRRAG"
     gene            complement(4600..4878)
                     /locus_tag="VIX62_04845"
     CDS             complement(4600..4878)
                     /locus_tag="VIX62_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859592.1"
                     /translation="MFVFILILLLLAAIFGVLGAVLKVAAVLILSAFLAVAVLAAVAW
                     WALKRKARQIQRDYDTQRIQGSSRSTGTTVFVENEADPGELPARDDRY"
     gene            4994..5824
                     /locus_tag="VIX62_04850"
     CDS             4994..5824
                     /locus_tag="VIX62_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003895546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5859593.1"
                     /translation="MDEGFRSPLDADANAGAVAIVTGGGTGIGRATARELARTGASVA
                     ICGRRPEPLERVRAELETDGRNVLAMPCDVREPAQVAVFLDAVDDRFGAVDILVNNAG
                     GQFAAPAEAIELKGLRAVHRLNVDAPWHLTHEVATRWMIPRRRGVVFFLGFSPRRGIP
                     MMTHSSMARSALETMAASLSNEWSRYGVRAICVAAGLIQTEGMLQYGGQEVIDGFADL
                     VPMRRAGLPEEVASTIAFLASPGGGYVTGSTVIVDGGADAWGQGMAPPPVEPPADPAG
                     "
     gene            5923..7374
                     /locus_tag="VIX62_04855"
     CDS             5923..7374
                     /locus_tag="VIX62_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5859594.1"
                     /translation="MADALKNYIDGKWVDAIDGGRFDVFDPATGEVIATAPDCQPADV
                     ERAVDAARRTFDDGTWWPGTSERERGRILLRAADIMRRELDRLAELESLDCGKPIGEA
                     RDDLEEVVFMWEYYGGWATKIYGDIPPVGPDAMSLVIKEPMGVAALITPWNYPMPMAV
                     QKVAPAIAAGCTMVLKPAEQTPLTALEIPKILEEAGLPAGVLNVITGFGETAGAPLVT
                     SPKVDKVGFTGSKDVGKIIMRSGADTLKRVTLELGGKSPNIVFADADFETAIEGSANG
                     IFWNQGEICSAGSRVFIERSIYDDALNALVAHADAVTMGSGRAEGTTMGPLVSKEQQE
                     RVQEYIEIGKGEATLATQGVLPDDPSLANGYFVPPTIFADVDNSARIAREEIFGPVMS
                     VIPFTSAEEVVELSNDNEYGLAAAVWTRDIKKAINTAKSLRAGIVWINDTQPAPTEAP
                     WGGYKQSGIGRELGRDGVDDYMEAKHIYINLSE"
     gene            complement(7392..8591)
                     /locus_tag="VIX62_04860"
     CDS             complement(7392..8591)
                     /locus_tag="VIX62_04860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859595.1"
                     /translation="MDEPVAGPPTSPEAPDRTTSNRRDIAPLLIGYWGFGQFWGVWVI
                     LVSDFQAFHGINDSRMGLYYTLLSVTAVSVMAFLAPHLQRFELSTSVPLCLMTLSLGS
                     LVIAAQPSSAIFVGIILVGMGNGLIDVYLNVAAQRVEIRSGKPVLQWMHACYALGGIT
                     GAAVAGLTLTAGMDYRVGLLFAGVALAGTSVVASRLLPKEGVGGSVETSFSITALFRN
                     KTLWVPALVVLSAFLVEGSMDTWSGRYLRVELGATAGVAALVFIAFSTALLLGRLFAG
                     RVLFGLGPRATILVAGFGAAIGGAIAAITDSATVVACAYLLMGFTIAAAAPAGFSLVN
                     VPDEDATNAIAAVSTVGYTGFIWSPPILGWVADTFSLRASVSVIVVATLGIVVGGMLA
                     PKVVVRR"
     gene            8672..9793
                     /locus_tag="VIX62_04865"
     CDS             8672..9793
                     /locus_tag="VIX62_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5859596.1"
                     /translation="MASGEVRLVDLVKRFGDFTAVAGINLDMPSGEFFSLLGPSGCGK
                     TTTLRMIAGFERPSEGQILLDGNDMAQTPPHKRNVNTVFQNYALFPHLTVADNVAFGL
                     KYKKASKQETTERVGSALELVQMSQFANRRPSQLSGGQQQRVALARALILNPAVLLLD
                     EPLGALDAKLRKRLQIELKALQEEVGITFIYVTHDQEEALTMSDRIAVMSQGRVEQVG
                     GPKEIYEEPATAYVADFLGVSNLMDASASGATTGGCKVQLGEFTLIAGQGEEDATGDC
                     KVTIRPERVNLEAQATSGENRIPAMVERVVYVGSVLQTIIHLASGQTIQAWLPNDEAA
                     DVPRSGAPITVHFPREALRVLPDAGTPVVAGELELEIDD"
     gene            complement(9902..10804)
                     /locus_tag="VIX62_04870"
     CDS             complement(9902..10804)
                     /locus_tag="VIX62_04870"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859597.1"
                     /translation="MGATAGTAAEATKRQSWFKNPWRKPYFLEGVTVLYLLWSLVPVV
                     IAVIFSFNAGRSRSAWQGFSLRWWTADPFDSLFHNPSLRQAMIQTFKLSSVASVVAVV
                     LGTLFAIGIDRWHGRPARGANFTMLLSFVVPEIILGVSLFLLFTQLLKNTVTLGTGAQ
                     WLGLIAFQISYPVIIVRARLLTIGPEYEEAAMDLGATPTQAIRKALLPLLFPAILASF
                     AVTFADTVDDFVTVRYLSGKADSEPLSVKIYGAARASPTPAVNAAATVMLIATLLVIV
                     VGYLIYRRISRGTGDSDVGSFAQL"
     gene            complement(10810..11793)
                     /locus_tag="VIX62_04875"
     CDS             complement(10810..11793)
                     /locus_tag="VIX62_04875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859598.1"
                     /translation="MAATSTSDPPAPTRRQKKQQAKAQASANEASGAWYPRWYWPSFT
                     VPGIVWLALLFLLPFFTVLSVAFGTVDPIFRQPLPVYQPWWWSFATFNETLSKFYSGD
                     AIYQGPLIRTFAYVFTASFICLVVGYAVAYYAARYAGKYRVLVLILLISPFWISYLMR
                     IYAWQSLLQPDGYVNDILTGLHIVGEPVNWLAGKHITVILGLVYGYIPFMILPLFGSL
                     DRINQSMLEAGRDLGGSPFETFRRVTLPLSRPAILAGLVIVSLPMFGDYYTNNLLGTT
                     QTSMFGNLIDNAVTQAGRGPEAGSLVIILMIIVIVPMLYYLHETKRAAAAR"
     gene            complement(11802..>12870)
                     /locus_tag="VIX62_04880"
     CDS             complement(11802..>12870)
                     /locus_tag="VIX62_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5859599.1"
                     /translation="NGEPIPTDTPIEAGATLQVYNWSDYFYRKVLAEFEEQYDVKIEW
                     TTFNNMEEGIQKLVAGQVKPDVFFPTTDYISRLVQTDLLQPLNHELLPNMANNVWPSF
                     SDPGPWYDQGWQYTVPYVIYTTGVAYRRDHIDDSVAQEKGYDLLWDPEYTGKISYYDS
                     YRDALGMAMLRNGDLDPNSGDPAVIDRAREAVSEIVGPLQGKLTINGTYAKLPEDEYY
                     VTQAWSGDIVGAQFYLPRGTGEEVLGYWYPDDNKGLIGNDTMVIPTTAQNPRLAHEFL
                     NFFLDKKWGYENFVNYNGYQPPFTTIEPDTLIANNVVPENLNRAVVTEEMFTKAYVQG
                     QLSADVDDLWLDAWDQIQAGS"
BASE COUNT         2055 a         4352 c         4464 g         1999 t
ORIGIN      
        1 tgcgcatacg tatttcgtgc ccgtctccca taccgaacgt gcgcggtcgg tcctcggacc
       61 cgagcccgta ctggcggtgg agttgaccgc gatccacgcc accgatccca cacgcgcgcg
      121 gcacctcgcg cgcgcctggg cccggcacta cctcgagctg ccgaactacg ccaagaacct
      181 cgtgcggatg ggcttcagcg aggacgaagt gaccggcgac ggcagcgacc ggttgatcga
      241 cgcgacgatc gcctggggcg atgccggcgc ggtggccgcc agggtgcgtg agcacctcga
      301 tgcgggagcc gaccacgtgt gcgtccagta catcgacgcc gatgacgcgg atctgtgcct
      361 gccggcgtac gccgaactgg ccgagtccgt cctcgcgtga gggtcaacgt gaagcccggg
      421 cccgcaggcg agcctggacc ccgtcgctcc gcagttcccg gagcgcgtcc ctcgcgatcc
      481 atctcgcgct gcggctcggt gaggccgcca gccgttcgcc cgtgtcgatc gcggcggcgt
      541 tcagcgcgag atcgcgcttc ccgatctggc gcagcgccca gttcaccgcc ttcttgacgt
      601 agttccgttc gtcgaccgcc ccgcgctcga tctctaggag ccatcccagc cagaaggcgt
      661 cgtcgtgatc gtctcgcgcg cgggcggcgt gctcggccac gatcgagaac cccgcccgct
      721 tcacgaactc ctcctcacgc cgcacccacg cgcgcgcccg gtcgaaggaa tgcggcgtct
      781 cggtgaacag gttgccgcag acctggtcgc agaggtccca cgaatcgaag tcggtcgccc
      841 aagcctccat ctgctccacg gtgacctgcg acgggtcgtc gaccatcgac gcgaggatcc
      901 tcgcctcgtg gatcttcgtc gtccacaggt cgagcgcgag cgcatggtcg ccgcggtggc
      961 gcctggcgag ccgccggcag ttcgggatgc tgaccccgag cgcccggtcg atggtgatcc
     1021 ccaccctggc catgcccggc cgccgcgatg ggtcggcgac cgaccggagt tcgtccacca
     1081 cctgccggct ggcggccttc acggctcgcg agctcccgtg atcgccgacg gatccaccga
     1141 acacgctcgc tggccgagca tcctgccgag ccgggtccgg tgtcgaacca cgaacccgta
     1201 catccgctcg gtcgcggcag ggaaggtggc ggtgagggtc gcgaccggcc ggccaccggg
     1261 gagccggcgc atcacgcgcg ggatcgccgc tcccgccgac cagatgcggc cgtccggttc
     1321 gacgacgtgc cacgaggcgt agcgggccgc cggatccatc gcgtcgaggt ccccgcctgc
     1381 ctcggccgtt tggatcgatc ggaacgtcag ccgatcgcgg cggtcccagc gccggagccg
     1441 gtccgccgtc caccggcaga ccccgcagtc ctcgtcgtag agcacgatcg cccggtccat
     1501 acctcgatgc tacggcgggt ttccgcttcg ccctgtggcc gttcaggccg gaaccgtgac
     1561 gaaccgcagg cctttcttcc ggatgccgcg gatcagatcc ggcaaggccg cgatcgtgtg
     1621 gccggcgtcg ccgccgccgt catggagcag gatgatcgac ccggcctcca cctgtttgag
     1681 caccttcttc acgatatcct tgcgcgtgcg gctcgatcgc cagtcctggg ggtcgacgct
     1741 ccacatcacg atccgcatcc cctgccgacg ggcttcatcg atcacgaagt cgtcgtagct
     1801 gccgccgggc ggccggaaca gtgtgggatg cacgccgacc tcgcctagct gcgcgttcgt
     1861 gtcggccatc tcggcggtga tgtgcgcctc ggtcatgtcg gccaggggtg ggttctcggg
     1921 atggtcgaac gaatggttgc cgatctcgtg gccagctcgc ttgacgcggc gaaccagctc
     1981 cgggtagcgc tccacctcgt agcccaccat gaagaacgtg gccttcacgt ggaagcggtg
     2041 caggatcgag agcacgcgtt cggtgtggac cggccagggc ccgtcgtcga aggtcagcgc
     2101 caccgcgccg ggcgtttcgc cgtgcccgcg ggggttctcg cgcaccgaga ccacgtcccc
     2161 cgagatgcgg ccctcgatgg tgatctcccg gatcgggtac ctggtcaacg tccgttccgg
     2221 gttcgctacc tgtggctcct tgatgtcgac gacgacccgt cgcgtgcctt cgacgcgatc
     2281 gtgccggtcg agcacctgga tggcatcacc ctgacggagc cgggtgcccg gcgcggcggg
     2341 cgtcccgttg aggacgatgt gaccccggcg tgcgagcgga tcgagcacct cgccggagac
     2401 cgacagcagc cggccgtcgg cggccgagag tccgagctct gcgatcacgc ccgcgaacgt
     2461 cgtgccgggt gcgaacgcgt gcgtggtgcc gtcgaccgtg atgcggatcg ggggcggggg
     2521 cgtcggtgtg gagccgccgc acatcggcag tgcggagagc acgacggcga tgaggacgag
     2581 agacgaacga cggtggggaa gcgatgaccg cacatgcaac cccatcatcc ttgcacgacc
     2641 gtcaagcatc ccgcccaagc atcccgcctg gcatcatgaa cgcatgtcga ccaggaaggt
     2701 gaagcggccg cgcgcggggc gtcacccgaa ccaggacccc gcccccccgc ctcctcgacc
     2761 cgagacgccg gctgcctggc tcatcgccgc cgacacggtg gcgctggtgg tgttcgtgct
     2821 ggcggggatg cgttcacatc acgagggctc ggcggcgtgg atcttcggcc gcaacgcgat
     2881 cccgctcctg atcgcgtggt tcgcggtggc ggcactgctg aaaacctatc ggacgccggg
     2941 cttcatgatc gtggccaaga cctggatcgt ggccgtcccg atcggactca ccgtccgttc
     3001 gctgtgggtg ggttcgccca cggggtggcg cttcgtcgtg ttcctggcgg tggggctggg
     3061 gttcacgatg ctcttcctgc tgatcgggcg cctgttgctc gcgctgatca ccgggcgggg
     3121 ctacccgcaa cggcgccgca cgtgagcacg cgcctgtcga tgcccgaccc gggcgcggcg
     3181 cggagcttct ggctccaaga ggccctcggg gcggaccccg gcgaaccgtg cccgcccctc
     3241 gaggcgcacg tggtcgccga cgtttgcatc gtcggcgggg ggttcgccgg gctctggacg
     3301 gcgatcgagc tctcggcccg cgaacccacg atgcgcatcg cgctgctgga gcaggacatc
     3361 tgcggcggcg gcgccagcgg gcgcaacggt ggtttcttct cctcgtcctg gtgggacgtc
     3421 gaggcgctgt gcgggctctt cggcgacgac gatggactgc gctatgccca cgcggtgtcg
     3481 gacacggtcg gtgaggcggg gacgtggctc gccgaccatg acgtcgacgc gtggttccac
     3541 cacgacgggg tcctggggat ccgaacgggg acctggcagg aggggcccgg tggcgagggg
     3601 ccggcggcgt tcctggaggc gcgtggactc ggcgatcgga tgcgaccgct caccgtggag
     3661 caggctcgcg agatcgccga ctcaccgcgg atcacgggtg ctcggttcat cgatgacagc
     3721 gcgatcgtgc agcccgcccg tctggcccgt ggcctgcgac gcatcgccct ggagcgcggt
     3781 gttcggatct tcgaacgcac cacggtgacc ggtctcgatc ggtcgcggcc ggcggtggtg
     3841 cgcaccgaac acggcgcggt caaggccgat caggtggtgc tggcgatcgg cgcctgggcg
     3901 gccggctggc cggggttccg ccgaagcttc ggcgtgatcg ccgaccacat ggtggtcacc
     3961 gagcccatcc cggagcggct ggcagagatc ggctggacct cgttcgcggg gatcgccgac
     4021 ggccgggacc tcctcttcta cctgcgcccc accgacgacc atcggatcgc gatcggtggc
     4081 gggggcttgg cggtcgtgtt cggcggccgt gcatccggac gcagcgcgac ccacgaccgt
     4141 cgcgtcgccg acgtcgcggc tcgcggcctg ctgtggctgt tcccgcagct ggagggcgtc
     4201 cggttcacgc acgcctgggg cggccccatc gaccagacgc cgtcgttcct gccgttctac
     4261 cgaacgctcc agcccggcaa cgtgcacgcc gggctcggct tctccggcca cgggctctcg
     4321 cagacgatgg tcggctcccg catcctggcg tcgatggtgc tgggcgttga ggaccgttgg
     4381 acctcgctgc cggtggtggg gggcgaactc ggcaaggccc cacccgaacc gttccgctat
     4441 cccgcgatca aggcggcggg ttgggcgctg gaacggggcg accgccgggc cgacgcgggg
     4501 cgcccccggg gctggttgga tgccaccgtg ggtgcagcgc cgcaggcctg gcgcgaccgg
     4561 ttggtggctc gggcgtcgcg ccgcagggcc ggctgaagct cagtaccggt cgtcgcgagc
     4621 ggggagctcg ccggggtcgg cttcgttctc cacgaacacc gtcgttcccg tcgacctgga
     4681 cgagccctgg atgcgctgcg tgtcgtagtc gcgctggatc tgccgggcct tccgcttgag
     4741 ggcccaccac gcgaccgccg ccaacacggc gacggcgagg aacgccgaca ggatcagcac
     4801 ggcagccacc ttcaacaccg cgcccagcac gccgaagatg gcggccagca gcaacaggat
     4861 caggatgaag acgaacatct cgtctcctag cctacgccgc cgcgcgagat tcgttcggcc
     4921 gcgcgcagcc ggccgcccgc gcggcgagca cgcgccggca accggggggt ctccgctccg
     4981 ccatactgcc gcgatggacg aaggattccg ttcgccgctg gatgccgatg ccaacgccgg
     5041 cgcggtcgcc atcgtcacgg ggggtggcac cggcatcggc cgggccaccg cgcgcgaact
     5101 cgcacgcacg ggggcgagcg tcgcgatctg cgggcgccgt ccggagccgc tggagcgggt
     5161 gcgcgccgag ctggagacgg acgggcggaa cgtgctggcg atgccctgcg acgtgcgcga
     5221 gcccgcgcag gtcgccgtgt tcctcgacgc ggtcgatgat cgcttcggcg cggtcgacat
     5281 cctggtgaac aacgccggcg gtcagttcgc cgcgcccgcc gaggcgatcg agctcaaggg
     5341 cctgcgcgcc gtgcaccggc tgaacgtcga cgcgccctgg cacctcacgc acgaggtcgc
     5401 cacccgctgg atgatcccgc ggcgccgagg cgtcgtgttc ttcctgggct tcagtccccg
     5461 gcgagggatc ccgatgatga cgcattcgtc gatggcacga tcggccctcg agacgatggc
     5521 ggcgtcgctg tcgaacgagt ggtcccgcta cggggtccgc gcgatctgcg tcgccgccgg
     5581 gctgatccag accgagggca tgttgcagta cggcggccag gaggtcatcg acgggttcgc
     5641 cgatctggtg ccgatgcgac gggccggcct gcccgaagag gtcgcctcga cgatcgcctt
     5701 cctcgcttcg cccggtggtg gctacgtcac cggctcgacg gtgatcgtgg acggcggcgc
     5761 cgacgcctgg ggtcagggga tggctccgcc gccggtcgaa ccccccgccg acccggccgg
     5821 gtagcgctcc gaggcgactt ccggcggccg cgtcgctcgt agtaggtttg ccggcacagc
     5881 ccggcggccc tcgcggccac gttccctcag gaggggttcc tcatggcaga cgctctgaag
     5941 aactacatcg acggcaagtg ggtcgacgcg atcgacggag gacggttcga cgtgttcgac
     6001 ccggccaccg gcgaggtcat cgcgaccgcg cccgactgcc agcccgccga cgtggagcgc
     6061 gcggtggatg ccgcccggcg caccttcgac gacggcacct ggtggccggg tacgtccgag
     6121 cgtgagcggg gccgcatcct gttgcgggcc gccgacatca tgcggcgcga actcgaccgg
     6181 ctcgccgaac tcgaatccct cgactgcggc aagccgatcg gcgaggcgcg cgacgacctc
     6241 gaagaggtcg tgttcatgtg ggagtactac ggcggctggg ccacgaagat ctacggcgac
     6301 atcccacccg tgggcccgga cgcgatgagc ctggtcatca aggagccgat gggggtggcg
     6361 gccctcatca ccccgtggaa ctacccgatg cccatggcgg tgcagaaggt ggcgccggcg
     6421 atcgccgccg gctgcaccat ggtgctcaag cccgccgagc agaccccgct gacggcgctg
     6481 gagatcccga agatcttgga ggaagccggg ctcccggccg gcgtgctgaa cgtgatcacg
     6541 ggattcggtg agaccgccgg tgctccgctg gtcaccagcc ccaaggtgga caaggtgggc
     6601 ttcaccggtt cgaaggacgt cggcaagatc atcatgcgca gcggtgccga cacgttgaag
     6661 cgcgtcacgc tggagctggg cggcaagtcg cccaacatcg tgttcgccga cgccgacttc
     6721 gagaccgcga tcgaaggcag cgccaacggc atcttctgga accagggcga gatctgctcc
     6781 gccggctcgc gggtgttcat cgagcgttcg atctacgacg acgcgctgaa cgcgctggtg
     6841 gcccacgccg atgcggtcac gatgggttcg ggtcgcgccg agggcaccac gatggggccg
     6901 ctggtgtcga aggagcagca ggagcgggtg caggagtaca tcgagatcgg caagggcgaa
     6961 gcgaccctcg ccacccaagg tgtcctgccc gacgatccgt cgcttgccaa cggctacttc
     7021 gtgccgccga cgatcttcgc cgacgtcgac aactccgcgc ggatcgcacg cgaggagatc
     7081 ttcgggccgg tgatgagcgt gatcccgttc acgagcgccg aggaggtcgt cgagctctcg
     7141 aacgacaacg agtacggcct ggcggcggcg gtgtggaccc gcgacatcaa gaaggccatc
     7201 aacaccgcga agtcgctgcg tgccggcatc gtgtggatca acgacacgca gccggcgcct
     7261 accgaggcgc cgtggggcgg gtacaagcag tcggggatcg gacgcgaact cggtcgcgac
     7321 ggggtggacg actacatgga ggccaagcac atctacatca acctgtccga gtaaccccgg
     7381 acgggctcgc ctcagcgcct gaccacgacc ttgggcgcca gcatgccgcc caccacgatc
     7441 ccgagggtgg cgaccacgat cacgctgacc gacgcccgga ggctgaaggt gtcggccacc
     7501 cagcccagga tgggcggact ccagatgaac cctgtgtagc ccaccgtgga gaccgccgcg
     7561 atcgcgttgg tcgcgtcctc gtcggggacg ttgaccaggc tgaatcccgc cggggccgcg
     7621 gcggcgatcg tgaagcccat caacaggtag gcgcacgcga cgaccgtggc gctgtcggtg
     7681 atggcggcga tcgcgccgcc gatggccgct ccgaagcccg ccaccaggat cgtggcgcgc
     7741 ggccccagcc cgaacaggac ccgtcccgcg aacagcctgc ccaacagcaa cgccgtggag
     7801 aacgcgatga acaccagcgc ggcgacgccc gcggtggccc cgagctccac ccgcaggtag
     7861 cggcccgacc aggtgtccat cgacccctcg accaggaacg ccgacaacac gacgagcgcg
     7921 gggacccaga gggtcttgtt gcggaacagg gccgtgatgg agaacgaagt ctcgaccgaa
     7981 ccaccgacgc cttccttcgg cagcagcctc gacgcgacca ccgacgtgcc ggccagggcc
     8041 acgcccgcga acaagagccc gacgcggtag tccatcccgg ccgtgagcgt gaggccggcc
     8101 accgcggcgc cggtgatgcc gcccagcgcg tagcaggcgt gcatccattg caggacgggc
     8161 ttgccgctgc ggatctccac ccgctgggcg gcgacgttga ggtagacgtc gatcagaccg
     8221 ttgcccatgc cgaccaggat gatccccacg aagatcgccg aggacggttg cgccgcgatg
     8281 accagtgaac ccagcgacaa cgtcatcaga cagagcggca cgctggtcga cagctcgaac
     8341 cgctgcaggt gtggcgccag gaaggccatc acggagaccg ccgtcacgga cagcagcgtg
     8401 tagtacaggc ccatccggct gtcgttgatc ccgtggaagg cctggaagtc cgagacgagg
     8461 atgacccaca cgccccagaa ctgaccgaat ccccagtagc cgatcagcag cggggcgatg
     8521 tcgcggcggt tcgaggtcgt tcggtccggc gcctcaggcg aggtgggcgg acccgccacg
     8581 ggttcatcca tccgggcatc atgccacgga cgatcggagg gctttgcaca cttgacccta
     8641 ggtggcgggg ctgggctata gtccgcccac gatggcgagt ggagaggtgc gactggtcga
     8701 cctcgtgaag cgtttcggcg acttcacggc ggtggccggg atcaatctgg acatgccctc
     8761 gggtgagttc ttctcgctcc tggggccctc cggctgcggc aagaccacca ccctgcgcat
     8821 gatcgccgga ttcgagcgcc cgtcggaagg tcagatcctg ctcgacggca acgacatggc
     8881 ccagacgccg ccccacaagc gcaacgtcaa caccgtgttc cagaactacg cgttgttccc
     8941 gcacctcacc gtcgccgaca acgtggcgtt cggattgaag tacaagaagg cgagcaagca
     9001 ggagacgacc gagcgcgtgg gcagcgcctt ggaactggtg cagatgagcc agttcgcgaa
     9061 ccggcgtccc tcccagttgt ccggtggtca gcagcagcgc gtcgccctcg ccagggcgct
     9121 catcctgaac ccggcggtgc tgctgctcga cgagccgctc ggcgccctcg acgccaagct
     9181 gcgcaaacgc ctgcagatcg agctgaaggc cctccaggaa gaggtgggga tcaccttcat
     9241 ctacgtcacg cacgatcagg aagaggccct cacgatgagc gatcgcatcg ccgtgatgag
     9301 ccagggtcgt gtggaacagg tgggcgggcc gaaggagatc tacgaggagc ccgcgacggc
     9361 ctacgtcgcc gacttcctcg gtgtgtcgaa cctgatggat gcctccgcgt ctggagccac
     9421 cacgggtggc tgcaaggtgc aactgggcga gttcacgttg atcgccggtc aaggtgagga
     9481 agacgccacc ggcgactgca aggtcacgat ccggcccgag cgcgtgaacc tcgaagcgca
     9541 ggccaccagc ggggagaacc ggatccccgc gatggtcgag cgcgtggtct acgtcggctc
     9601 ggtgctccag accatcatcc acctggcctc gggccagacg atccaggcct ggttgccgaa
     9661 cgacgaagcg gccgacgtgc cgcggtccgg ggcgccgatc acggtccact tcccccggga
     9721 agcgttgcgg gtgctgccgg atgccggaac ccccgtcgtc gccggtgagc tcgagctcga
     9781 gatcgacgac tgaccgagcg ctccggcccc ttctcagcga ccaccctcta cacacgaacg
     9841 gccgccgcgt ccggcgatgg tgccggacga gacggccgtt cggtgacgct gcggagccgg
     9901 gctacagctg ggcgaagctg cccacgtcac tgtcgcccgt gccgcgtgag atccgccggt
     9961 agatcagata ccccaccacg atgaccaaca gcgtggcgat gagcatcacg gtggcggcgg
    10021 cgttgacggc gggcgtgggg gacgcgcgag ccgcgccgta gatcttcacc gacagcggct
    10081 cggagtcggc cttgcccgac aggtaccgga ccgtgacgaa gtcatcgacg gtgtcggcga
    10141 acgtcaccgc gaagctcgcg aggatcgccg ggaacaacaa gggcagcaac gccttgcgga
    10201 tcgcctgcgt gggcgtggcc ccgaggtcca tcgccgcttc ttcgtactcg ggcccgatcg
    10261 tcagtaatcg cgcgcgaacg atgatgaccg ggtaggagat ctggaacgcg atcaacccga
    10321 gccactgcgc gcccgtcccc agggtgacgg tgttcttcag cagctgcgtg aacagcagga
    10381 acaacgacac gccgaggatg atctccggca ccacgaacga cagcagcatc gtgaagttgg
    10441 cgccccgcgc cgggcgcccg tgccaacggt cgataccgat cgcgaagagc gtgcccagca
    10501 ccacggcgac caccgacgcg acgctcgaga gcttgaacgt ctggatcatc gcttggcgca
    10561 gcgacgggtt gtggaacagg gagtcgaagg ggtccgccgt ccaccaccgg agcgagaagc
    10621 cctgccacgc gctcctcgag cgaccggcgt tgaacgagaa gatcacggcg atcaccaccg
    10681 gtaccagtga ccagagcagg tagagcaccg tgacgccctc caagaagtag ggcttgcgcc
    10741 acgggttctt gaaccagctc tggcgcttgg tggcttcggc cgcggtgcct gcggtggcgc
    10801 ccatctcgct catcgtgctg ccgccgctcg tttggtctcg tgcaggtagt agagcatcgg
    10861 gacgatcacg atgatcatca ggatgatcac cagcgagccc gcctcgggtc cgcggcctgc
    10921 ctgcgtcacg gcgttgtcga tcaggttgcc gaacatcgag gtctgcgtcg tgcccagcaa
    10981 gttgttcgtg tagtagtcgc cgaacatcgg caacgacacg atcaccagcc ccgccaggat
    11041 ggccggcctc gacagcggca gcgtgacgcg tcggaacgtc tcgaacgggc tcccgcccag
    11101 atcgcgtcct gcctccagca tgctctggtt gatgcggtcc agcgagccga acagcggcag
    11161 gatcatgaac gggatgtacc cgtagaccag cccgaggatc acggtgatgt gcttgccggc
    11221 gagccagttc accggttcgc cgacgatgtg caggccggtc aggatgtcgt tcacgtagcc
    11281 gtccggctgg agcaggctct gccaggcgta gatccgcatc aggtagctga tccagaacgg
    11341 cgagatcagc aggatcagca ccagcacccg gtacttcccc gcgtaccgag cggcgtagta
    11401 ggccaccgcg tagcccacca ccaggcagat gaagctcgcc gtgaacacat aggcgaacgt
    11461 gcggatcagc ggaccctggt agatggcatc gcccgagtag aacttgctga gcgtctcgtt
    11521 gaacgtggcg aagctccacc accacggctg atacaccggg agcggctggc ggaagatcgg
    11581 gtcgaccgtg ccgaacgcca cggacaacac cgtgaagaac ggcagcagga acagcagcgc
    11641 caaccagacg atgcccggca ccgtgaacga gggccagtac caccgcgggt accaggcccc
    11701 gctggcttcg ttcgctgacg cctgcgcctt cgcctgctgc ttcttctgcc gcctcgtggg
    11761 tgcgggcggg tcgctcgtgg aggttgctgc cacctcggtg actagctccc ggcctggatc
    11821 tgatcccacg cgtcgagcca caggtcgtcg acgtcggcgg aaagctgacc ctgcacgtag
    11881 gccttggtga acatctcttc ggtgacgacg gcccggttga ggttctccgg aaccacgttg
    11941 ttggcgatca acgtgtcggg ctcgatcgtc gtgaacggcg gctgatagcc gttgtagttc
    12001 acgaagttct cgtatcccca cttcttgtcg aggaagaagt tgaggaactc gtgggccagg
    12061 cgcgggttct gcgcggtggt ggggatcacc atcgtgtcgt tgccgatcag gcccttgttg
    12121 tcgtcggggt accagtagcc cagcacctct tcgccggtgc ctctcggcag gtagaactgc
    12181 gcgccgacga tgtcaccgga ccatgcctgg gtgacgtagt actcgtcctc gggcagtttc
    12241 gcgtaggtgc cgttgatcgt gagcttgccc tggagcggtc ccacgatctc gctgaccgcc
    12301 tcgcgggccc ggtcgatcac ggcggggtcg ccactgttgg gatcgaggtc gccgttgcgg
    12361 agcatcgcca tgccgagggc gtcgcggtag gagtcgtagt agctgatctt gcccgtgtac
    12421 tcggggtccc agagcagatc gtagcccttc tcctgcgcga cgctgtcgtc gatgtggtcg
    12481 cggcgatagg ccaccccggt ggtgtagatc acgtagggaa cggtgtactg ccacccttgg
    12541 tcgtaccacg ggcccgggtc cgagaaggaa ggccacacgt tgttcgccat gttggggagc
    12601 aactcgtggt tcagcggctg gagcaggtcc gtctggacga gccgggagat gtagtcggtt
    12661 gtggggaaga acacgtcggg cttgacctgg cccgcgacca gcttctggat cccttcctcc
    12721 atgttgttga aggtggtcca ttcgatcttg acgtcgtact gctcttcgaa ctccgcgagc
    12781 accttgcgat agaaatagtc gctccagttg tagacctgca gcgtggcgcc ggcctcgatc
    12841 ggggtgtcgg tcgggatcgg ttcgccgttg
//
LOCUS       DATDBZ010000196         6182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_886026, whole genome shotgun sequence.
ACCESSION   DATDBZ010000196 DATDBZ010000000
VERSION     DATDBZ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886026"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VIX62_04885"
     CDS             complement(<1..673)
                     /locus_tag="VIX62_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY5859600.1"
                     /translation="MSQRRKVVITGIGPVTPVGTGVDEFWQGLSTGRNGVRTINAFDT
                     SDLPVSVAGYPADFVATDHLDAKEARRTDPFAQYAIASAKLAWADAGEPDVRSDRGAV
                     IYATGIGGVQTLLNQHDVLREKGAGRVSPFMVPMLMANAAGGHIAMHFGLTGPNYCTV
                     SACSSSNHAIGESMRLIRDGYSDLVIAGGSEAATIPLTVAAFAQMTALTKNPDPETAS
                     RPFDAD"
     gene            complement(715..963)
                     /gene="acpP"
                     /locus_tag="VIX62_04890"
     CDS             complement(715..963)
                     /gene="acpP"
                     /locus_tag="VIX62_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY5859601.1"
                     /translation="MTDRAEIETRVKKVLAEQLAVDEAQVVPDARFAEDLNADSLDLV
                     EAVLALEEEWSIEIPEEEMESVKTVGQAVDLVAGKLGS"
     gene            complement(1032..1772)
                     /gene="fabG"
                     /locus_tag="VIX62_04895"
     CDS             complement(1032..1772)
                     /gene="fabG"
                     /locus_tag="VIX62_04895"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY5859602.1"
                     /translation="MTTSKVAVVTGATRGIGRACAVALAEAGWTVAIGYRSDEAAAKE
                     ALSALESAGNPGLTVFLDTTDQASVQEAFRRVGDEVGNVTGLVNNAGFSQDGLLLKYS
                     MDNYDKVMNTNVKGAFLCSQAAMRGMLREKWGRIVNLSSAVALRGNAGQTVYAASKTA
                     LLGLTKSLAREIGGKGITVNAICPGLVDTEMTSHLDDRARDYYLDQTPIARTATLDEV
                     SSVVRFVMSEEASYVNGAVIPVDGGLTA"
     gene            complement(1769..2767)
                     /locus_tag="VIX62_04900"
     CDS             complement(1769..2767)
                     /locus_tag="VIX62_04900"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY5859603.1"
                     /translation="MTRYATIAGVGSSLPPRLVPNTWFETIVETNDEWIRDRTGIEAR
                     HFADEGTDTSDLAVEAARRAMESAGVTPEQLDMIIVATITGDTIFPATAVWVQQKLGV
                     RCPSFDVNAACAGFSYGLSSANAFITSGMADTILLIGAEVFSRILDYTDRGTCILFGD
                     GAGAAVVRASETPGFEGAVLGADGSAAEILWMPAGGTRTPASEATVAANDHTVRMPNG
                     REVFKRAVTEMGAATRELLEKSGLSIDDIDVLIPHQANARIMGAVVERLGIDPAKAIS
                     DVAEVGNTSAASIPVALDHAWREGRITPGDLVLFTSFGAGLTWGATLVRWTAESPA"
     gene            complement(2764..3690)
                     /locus_tag="VIX62_04905"
     CDS             complement(2764..3690)
                     /locus_tag="VIX62_04905"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY5859604.1"
                     /translation="MTFAVVFPGQGSQFEAMADPWTTHPAGQAVMEEASEAMERDIVA
                     GCHDPDALATTEFAQPALLACGVAAFRVLEAEGLRSERLAGAAGHSLGEFAALVAAGT
                     MVLPEALALVVVRGEAMQRAGEARPGTMSALIGADTETAEALCDEAREDGVLLVANQN
                     SPTQVVISGSVEAIERAEALAKERKIRAIRLNVAGAFHSPLMEPAVEPILRMLDDMEL
                     RTPAFPIAENVSGTLVDDPTELKELLGRHVVSPVLWERDAQSLAAAGATTFLEAGPGD
                     VLTKMAKRVVPGATALAVGTPEAAAAAVSSAP"
     gene            3841..4923
                     /locus_tag="VIX62_04910"
     CDS             3841..4923
                     /locus_tag="VIX62_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5859605.1"
                     /translation="MAERIQRSSSPTSKNQGTEGVLGFLGRDMAVDLGTANTLVYVRG
                     RGVVLNEPSVVAINTLTGAILAVGAEAKRMIGRTPSHIQAVRPLKDGVIADFDVTEKM
                     LRYFIQRVHKRRLLAKPRVVVCVPSGITGVEQRAVEEATIAAGSRRAYIIEEPMAAAI
                     GAGLPIHEPTGNMVVDIGGGTTEVAVISLGGIVTSTSIRIGGDELDEAIIQHVKKEYS
                     LLLGERTSEAIKLAIASVFPMPEELVAEIKGRDLVSGLPKTITITAEEIRRAIEEPVN
                     AIIDAIKTTLDRTPPELSADIMDRGIVLTGGGALLRGLDKRLRHETGMPVRIADTPLQ
                     AVVLGSGKCLEEFEVLQRVLVSPSRR"
     gene            4930..5787
                     /gene="mreC"
                     /locus_tag="VIX62_04915"
     CDS             4930..5787
                     /gene="mreC"
                     /locus_tag="VIX62_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY5859606.1"
                     /translation="MVTRQRPRATRLLVVVLVSISLAVITLDYRQQDGPLSALGTSTK
                     AAMAPMQRAVTNVTRPVGDFFSGLAHLPSLSKENQELKDELAAAHAEDAVNDLIQVQN
                     EELQGLLDLRATLDPEPIAAVVIANGLSNFEWTITIDKGSNDGIAKDQPVVTGSAEAP
                     MLVGRVVQVGPDSADVRLIIDRESAVAAVLKDSGEAGLIHGQGDADLRMTDVEPGTVT
                     EGDVEQVFTLGYEVTGQPGLYPPDLLIGQVSRTVPADNATEEFIEVRPAVDFATLQFV
                     LVLKARTEA"
     gene            5792..>6182
                     /gene="mreD"
                     /locus_tag="VIX62_04920"
     CDS             5792..>6182
                     /gene="mreD"
                     /locus_tag="VIX62_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY5859607.1"
                     /translation="MTRRVLSIIAIVVTALLLQSTVFAEFKFLGVRPELMYLVTIIIA
                     ILEGPQEGAVVGFAGGMAQDFMLNQPKGITALTLTLVGYAVGLARQYIVSPSPLLPTI
                     LVAAGTAAGLIFYQVVAFLLGQLDEPFS"
BASE COUNT          924 a         2132 c         2118 g         1008 t
ORIGIN      
        1 ggtcggcatc gaacgggcgc gacgcggtct cgggatcggg gttcttcgtg agcgcggtca
       61 tctgcgcgaa ggccgcgacc gtgagcggga tcgtggcggc ttcggagccg ccggcgatca
      121 cgaggtcgct gtacccgtcg cggatcagcc gcatggattc gccgatcgcg tggttggacg
      181 agctgcacgc cgagaccgtg cagtagttgg gaccggtgag cccgaaatgc atcgcgatgt
      241 ggccgccggc ggcgttggcc atgagcatgg gcaccatgaa cggcgacacg cgtcccgcgc
      301 ccttctcgcg cagcacgtcg tgctggttga ggagcgtctg gacgccgccg atgccggtgg
      361 cgtagatcac ggcgccgcga tcggagcgga cgtcgggctc gccggcgtcg gcccacgcca
      421 gcttcgccga ggcgatggcg tactgggcga acgggtcggt gcgccgagcc tccttggcgt
      481 ccaggtgatc ggtcgccacg aagtcggcgg ggtagccggc caccgagacc ggcaggtcgg
      541 acgtgtcgaa ggcgttgatc gtcctcacgc cgttgcggcc cgtggacaac ccctgccaga
      601 actcatccac gccggtgccg accggcgtga ccggtccgat cccggtgatg acgaccttgc
      661 ggcgttggct cacgggttcc cctgttcagc gacgcccggt gatggcgacg gcgctcagct
      721 gcccagcttg ccggccacca ggtcgacggc ctggccgacg gtcttgaccg actccatctc
      781 ttcttccggg atctcgatgc tccactcctc ctcgagggcc agcacggcct ccacgaggtc
      841 gagggagtcg gcgttgaggt cctcggcgaa ccgcgcgtcg ggcacgacct gcgcctcgtc
      901 cacggccagt tgctcggcca gcaccttctt caccctggtt tcgatctcag ctcgatcggt
      961 catgcgcgtt tcctcctgtg ttcccctccg gttccgccca ggctaaaggg cggaacgcca
     1021 tccgacacga ttcacgcggt gaggccacca tcgacgggga tcaccgcgcc gttcacgtac
     1081 gacgcttctt ccgacatcac gaaccgcacg acggacgaca cctcgtccaa ggtggcggtc
     1141 cgcgcgatcg gcgtttgatc gaggtagtag tcgcgcgcac ggtcgtccag atgcgaggtc
     1201 atctcggtgt cgaccaaccc cgggcagatc gcgttcaccg tgatgccctt gcccccgatc
     1261 tcgcgcgcca gcgacttcgt cagccccagc agcgcggtct tcgacgcggc gtagacggtc
     1321 tgtcccgcgt tgccgcgcag cgcgacggcg ctcgagaggt tcacgatgcg gccccacttc
     1381 tcccggagca tgccgcgcat cgccgcctgc gagcagagga acgcgccctt cacgttggtg
     1441 ttcatcacct tgtcgtagtt gtccatcgag tacttcagca gcagaccatc ctggctgaac
     1501 cccgcgttgt tcacgaggcc cgtgacgttg ccgacctcgt cgcccacgcg gcggaacgcc
     1561 tcttggaccg aggcctgatc ggtggtgtcg aggaagaccg tgagtccggg gttaccggcg
     1621 ctctcgaggg cgctgagggc ttccttggcg gcggcctcgt cgctccggta gccgatcgcc
     1681 acggtccacc cggcttcggc gagtgcgacg gcgcacgcgc gaccgatccc cctggttgcg
     1741 ccggtgacga cggccacctt cgaggtggtc atgcggggct ctctgcggtc caccgcacca
     1801 gcgtggcgcc ccaggtgagc ccggcgccga acgaggtgaa caggaccaga tcgcccggcg
     1861 tgatccgtcc ctcgcgccag gcgtggtcga gggccacggg gatcgaggcg gcgctggtgt
     1921 tgccgacctc ggcgacgtcg gagatggcct tcgcgggatc gatgcccagg cgttcgacga
     1981 ccgcacccat gatgcgggcg ttcgcctggt gggggatgag gacgtcgatg tcgtcgatcg
     2041 agagccccga tttctccagc agttcgcggg tggcggcccc catctcggtc accgcgcgct
     2101 tgaagacctc ccggccgttg ggcatgcgca ccgtgtgatc gttggcggcc accgtggcct
     2161 cggaggccgg ggtgcgggtg ccgcccgcgg gcatccacag gatctcggcc gcgctcccgt
     2221 cggcgcccag gaccgcgccc tcgaacccgg gggtctcgga ggcgcgcacc accgcggcgc
     2281 cggcgccgtc gccgaacagg atgcaggtgc cgcggtcggt gtagtcgagg atgcggctga
     2341 agacctcggc gccgatcagc aggatcgtgt cggccatgcc gctggtgatg aaggcgttgg
     2401 ccgacgacag gccgtaggag aagccggcgc acgccgcgtt cacgtcgaac gacgggcagc
     2461 gcacccccag cttctgctgc acccacaccg cggtggcggg gaagatcgtg tcgccggtga
     2521 tcgtggcgac gatgatcatg tcgagttgtt cgggtgtgac gcccgcggac tccatcgcgc
     2581 ggcgggccgc ttccaccgcg aggtccgagg tgtccgtgcc ttcgtcggcg aagtgccgag
     2641 cttcgatccc ggtgcgatcg cggatccact cgtcgttggt ctcgacgatc gtctcgaacc
     2701 acgtattggg cacgagacgg ggcgggagcg aggagcccac gccggcgatg gtggcgtagc
     2761 gggtcacggc gccgaggata ccgcggcggc ggccgcctcg ggagtgccca cggccagcgc
     2821 cgtcgccccc gggaccaccc gtttcgccat cttcgtgagc acgtcgccgg ggccagcctc
     2881 caggaaggtg gtggcgccgg ccgcggcgag cgactgcgcg tcgcgttccc acagcaccgg
     2941 cgagacgacg tggcgcccca ggagctcctt gagctcggtc ggatcgtcca cgagcgtacc
     3001 gctcacgttc tcggcgatcg ggaaggcggg cgtccggagc tccatgtcgt caagcatgcg
     3061 caggatcggt tccaccgccg gttccatcag cggcgagtgg aacgcaccgg ccacgttgag
     3121 ccggatcgcc cggatcttcc gctccttcgc gagcgcctcg gcgcgttcga tcgcctcgac
     3181 gctgccgctg atcaccacct gggtggggga gttctggttg gcgaccagca ggaccccgtc
     3241 ctcacgcgct tcgtcgcaga gcgcctccgc ggtctcggta tcggcgccga tcagcgcact
     3301 catcgtgccg ggtcgtgctt cgccggctcg ttgcatcgcc tcgccccgca ccacgacgag
     3361 ggcgagcgct tcgggcagca ccatcgttcc ggccgccacc agcgccgcga actcgcccag
     3421 ggagtggccg gccgcccccg ccaggcgctc cgagcgcagg ccctcggcct ccaacacccg
     3481 gaacgcggcc accccgcacg ccagcagggc cggctgcgcg aactccgtgg tagccaaggc
     3541 atctggatca tgacagccgg cgacgatgtc gcgttccatc gcctcggatg cctcttccat
     3601 gaccgcctgg ccggcggggt gggtggtcca cggatcggcc atggcctcga actgggaccc
     3661 ctgccccggg aacaccaccg cgaacgtcac gaaccctcct cggatacccg cctgaccgaa
     3721 ccgtggaagc ctaccggggc agtcacctgg tgtgccggtg agtgtgtcgg tgccgtggtt
     3781 tcgccctcgc gaagttgcca ggggggtgcc cctcctgttg aatgacctgc gagtcccatc
     3841 atggccgaga ggattcaacg atcttcttct cccacgtcca agaaccaggg gacggagggt
     3901 gtactcgggt tccttggtcg cgatatggcc gtcgacctcg gtaccgcgaa cacgctggtg
     3961 tacgtgcgtg gacgcggtgt cgtgctcaac gagccaagcg ttgtcgcgat caacaccctc
     4021 actggagcaa tccttgccgt gggtgccgag gccaagcgca tgatcggccg caccccgtcg
     4081 cacatccagg ccgtgaggcc cctgaaggac ggcgtgatcg ccgacttcga cgtcaccgag
     4141 aagatgctcc ggtacttcat ccaacgtgtg cacaagcgtc gcctgctggc caagccgcgt
     4201 gtggtcgtct gcgtcccgtc cggtatcacc ggggtggagc agcgcgcggt cgaggaagcg
     4261 acgatcgcgg cgggttcccg tcgcgcgtac atcatcgagg agcccatggc ggcggcgatc
     4321 ggcgccgggc tcccgatcca cgagcccacc gggaacatgg tggtcgacat cggcggcggc
     4381 acgacggagg tcgcggtgat ctcgctcggc gggatcgtca cgagcacctc gatccgcatc
     4441 ggtggcgacg aactcgacga ggcgatcatc cagcacgtca agaaggagta ctcgctgctc
     4501 ctgggtgagc gcacgtccga ggccatcaag ctcgcgatcg cgagcgtgtt ccccatgccc
     4561 gaggagctcg tggccgagat caaaggccgg gacctcgtga gcggtctgcc caagaccatc
     4621 acgatcaccg cggaggagat ccgccgcgcg atcgaggaac cggtcaacgc catcatcgac
     4681 gccatcaaga ccacgctcga ccgaacgccc cccgagctgt ccgccgacat catggaccgc
     4741 ggcatcgtcc tcaccggcgg tggtgcgctc ctgcgcggcc tggacaagcg cctgcgacac
     4801 gagaccggca tgccggtccg gatcgcggac accccgctgc aagcggtcgt gctcggctcc
     4861 ggcaagtgcc tggaggagtt cgaagtcctg caacgggtcc tggtgtcacc gtcgcggcgg
     4921 taatccccgg tggtcacccg gcaacgcccc cgcgccacgc gcctgctggt ggtcgtgctc
     4981 gtctcgatct cactcgcggt catcacgttg gactaccgtc agcaggacgg cccgctctcg
     5041 gcgctgggca cctccacgaa ggccgccatg gccccgatgc agcgcgcggt caccaacgtc
     5101 acccggccgg tgggggactt cttctccggg ttggcccacc tgccctcgct ctccaaggag
     5161 aaccaggagt tgaaggacga actggcggcc gcgcacgccg aggatgcggt gaacgacctg
     5221 atccaggtcc agaacgagga actccagggc ctgctcgacc tgcgggccac cctggacccc
     5281 gagccgatcg ccgccgtcgt gatcgccaac ggtttgtcca acttcgagtg gacgatcacg
     5341 atcgacaagg gctcgaatga cgggatcgcc aaggaccagc ccgtggtgac gggatcggcg
     5401 gaggcgccga tgctcgtggg ccgcgtcgtc caggtgggcc cggactcggc cgacgtccgg
     5461 ctcatcatcg accgcgagtc ggccgtggcg gccgtcctca aggactccgg cgaagccggc
     5521 ctgatccacg ggcagggcga cgccgatctg cgcatgaccg acgtcgaacc cggcacggtc
     5581 accgagggcg acgtcgagca ggtcttcacg ctgggctacg aggtcacggg tcagccgggg
     5641 ttgtatccgc ccgacctgtt gatcgggcag gtgtcgcgca cggtgcccgc cgacaacgcc
     5701 accgaagaat tcatcgaggt ccgtcccgcg gtcgatttcg ccacgctgca gttcgtgctg
     5761 gtgctcaagg ccaggacgga ggcatgaggg agtgacgcgc cgtgtcctgt ccatcatcgc
     5821 catcgtcgtc accgcgctgc tgctgcagtc gacggtgttc gccgagttca agttcctggg
     5881 ggtgcggccg gaactgatgt acctggtgac gatcatcatc gcgatcctcg agggcccgca
     5941 ggaaggtgcg gtcgtcgggt tcgccggcgg tatggcgcag gacttcatgc tgaaccagcc
     6001 gaaggggatc accgcgttga cgttgacgct ggtgggctac gccgtcgggc tggcgcgcca
     6061 gtacatcgtg tcgccgtcgc cgctgctgcc cacgatcctg gtggcggccg gcaccgcggc
     6121 gggcctgatc ttctatcagg tggtggcctt cctgctaggt cagctcgatg aaccgttctc
     6181 ct
//
LOCUS       DATDBZ010000197         2595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1290830, whole genome shotgun sequence.
ACCESSION   DATDBZ010000197 DATDBZ010000000
VERSION     DATDBZ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290830"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /gene="rsmG"
                     /locus_tag="VIX62_04925"
     CDS             <1..514
                     /gene="rsmG"
                     /locus_tag="VIX62_04925"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012300313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY5859608.1"
                     /translation="PLELPRLWTRHVLNSALVAPLMRPGRVGDVGSGAGLPGLVLAII
                     RPDVSFTLIEPMERRVAWLERQVDELELTNVTVVRARAEESKLYDSLDQVTARAVSAL
                     RTLIPVTAPLLRAGGELVLMKGTGVDGEIGAAEKAIRKYKLRAVEVLVLGEGVVPASE
                     VTRVFRASVG"
BASE COUNT          378 a          914 c          850 g          453 t
ORIGIN      
        1 cccgctcgaa ctcccccgcc tctggacacg ccacgtgctc aacagtgcgc tcgtcgcacc
       61 cctcatgcgc cccggccgcg tcggcgatgt cggatcgggg gccgggcttc ccggactcgt
      121 gttggcgatc attcgacccg acgtgtcatt cacgctcatc gagccgatgg aacgccgggt
      181 cgcgtggctc gaacgtcagg tcgacgagct cgagttgacc aacgtcaccg tggtgcgtgc
      241 acgtgccgag gagtcgaagc tgtacgactc actcgaccag gtcaccgctc gtgccgtcag
      301 cgcgctgcgc acactgatcc ccgtgactgc tcccctactg agggccggcg gtgagctcgt
      361 cctcatgaag ggcaccggcg tcgacggtga gatcggcgcg gccgagaagg cgatccgcaa
      421 gtacaagctt cgtgccgtcg aggtgctcgt gttgggcgag ggcgtcgttc cggcgagcga
      481 ggtcacccgg gttttccggg catcggtcgg ctgagcggca tggttcggcc cggccgtttc
      541 ggcggcgacg gccgtcggcg acaactcggg tgagcggttg acgatcgtgt tcgtcggggc
      601 gcccgcgccc cgggagggat ctggggtgtg actcgtcacg ccgcttcatt cggcgtttca
      661 cgtgaaacat tgctcgcggc gagtctctag cgcggtcaat ggcgttcact cgtgacggcg
      721 atctgctgcc gtgaatccgt cttcttgacg cagtggtgcc cgcccacgct ttcatccgtt
      781 tcgccgacgt caccctgctg ccctcgctca tatcgatgaa gcatcttcgc tcgggtcttc
      841 gctcgggtct tcgctcgatc ggctgaccac gccgcgccca ggaagagcgt cactcgcttg
      901 cgccgctccg ccgcgcccag gttccggggt gtgatctcgg gctccttgtt ggtgtgcggt
      961 gtaagcacca cgacctcgga agcgacggtc gagctcgccg ggggtcgagc gccttcttcg
     1021 cggcaccagc gcgcctccgc atcggcgttc gatgggcaac gcagcggtct cggccaagcc
     1081 gagtgcagga cttcaggcgc gccgtcggcg tgtacggagg gcagacgcgg gtccccggcc
     1141 gcgttcctgc actggcaaat gcggaccttc gggccgcctc ggtccgcatg cgccgtgccg
     1201 acctccgggc ggcccgtcct gcacttcgtc gtcgttgcac accggcgccc acccgcacgc
     1261 ggtgcccgga cgggtgggcc gagccctccc ccggccatca ccgtcgagtc agcgtgacgc
     1321 tttcgattcg ttcgtgatcg ggccggcgaa cacgtcgtcc tcctggcgtc tgattcgcag
     1381 cccgccgaca cgcaatgcgg aagcctgtca ttgcgcccca ggcgccgcga cgagcgtgga
     1441 gtgccgaaag ccccgacatg ttccgaaccc tgccgctgcg cggtcggatc cgttgtccgc
     1501 acgacgagcg atcgaccccg gcgcctggcc gagccgcgga agtgacgggt tgcgttgcgg
     1561 gcgtcgttcg ttgggcggag atgggggtgt ttcacgtgaa acatcacgcc gagggcgcac
     1621 cggtgcccgg ttgccagatc tgcgattcgc gaccaaaacc gtgatcccga gacagcgccg
     1681 cccatcctca gacccgcggt tggacctcac gcagaccctg ccttccgtgt ccgcctgccc
     1741 cacacggtgc cgatgttcag taatcgacgt cggctgcgcc atcggtccgg gtgcgcgcat
     1801 gttcacgtca aacatgggtg ccgcgaggcg ccgccaaacg ccggcgtgtt gtcgggaccg
     1861 accccttgag atcgcacagc gaccaatcca cccgaaccag tgcgtgattg ggaacaatgg
     1921 gatgcccttg aaggccgttc gcgcgccgca gttcggcctt tcgaagttga agagagcgag
     1981 caggactctg ccgatcgacc gccgttccga ggtccgaatc ggatcacccg gccctctcac
     2041 cgggcgtcct cgcacctacc tgcccgatcg accggggttg cgccgtggct cccacacgcg
     2101 ttcacattcg ctgcaaccgt gtgcccatcg accgggtcgc tccactctgg ctcggcgcgg
     2161 tggagcaact cgcacacacg gttcggcggc aacgggagcc cgacagggct acccgacgcg
     2221 tgtccccagt cgactccatc ggctcagcac cgtctggtcg gggccgcgga acccgtgaac
     2281 caacgaggct gcacacggaa cccgcgacgc gcccagcgag tgcgcgctcg atgcgtcgag
     2341 acgcctgttc aggtgcgatc tcgccccctc ccccatgccc tggttcacca aacccatcgc
     2401 tcgctcgggc aacgcggtcc gttgccgttc gcgtgacagg tcatgccgct ccagcggcga
     2461 attctcggtg cgcgggcgat tccgtgtggt cgggcgggag gagggacacg attgcacgct
     2521 cgagctcagt gcgaccaagc acaggccacg cgctgcctgc aggcattcga acccgacgca
     2581 cggaccgccc gttgc
//
LOCUS       DATDBZ010000198         3327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_474532, whole genome shotgun sequence.
ACCESSION   DATDBZ010000198 DATDBZ010000000
VERSION     DATDBZ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474532"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VIX62_04930"
     CDS             complement(<1..569)
                     /locus_tag="VIX62_04930"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5859609.1"
                     /translation="MTDHASEIADNRKLWDAWTAIHTTGDFYDVQRFQDDPNDVRIEP
                     WEQEEVGDVAGKTLLHLQCHFGLDTLSWARLGASHVTGVDFSEPAIAFAIELATETGL
                     ADRARFVVSNIYDLPGPLDGETFDIVYTGRGALSWLPDLRPWARSVAAFLKPGGIFYI
                     HEGHPVMWALDDDQTEPNGLQLGYDYWGG"
     gene            complement(566..1600)
                     /gene="speB"
                     /locus_tag="VIX62_04935"
     CDS             complement(566..1600)
                     /gene="speB"
                     /locus_tag="VIX62_04935"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY5859610.1"
                     /translation="MSDAPDFRLEHSELISEDEFSEFTSTDLPYYSDWRGSFEKLPWA
                     TDDESLRAANADIAIVGAPLDEGTSSRPGARFGPRAIRMAPTAWSSDYAWSIQLDVEP
                     YEHVTVVDAGDAPIVPTRFERGLRVIHEKVFRVASAGPIPIVLGGDHSITYPSVAGVA
                     RHVWPRTVGVIHFDAHADTGSDQWGNLYGHGEPMRRLIEEGWVAGRNFVQVGLRGYWP
                     EKSNWEWMREQGLRWHTMVEIEDRGSEAVIADAIAEALDGPDCIYLSVDIDVVDPGMA
                     PGTGTPESGGMLARELLRAVRQIVGAVDLVGMDVVEVSPPYDNAEVTAILAHRTVMEA
                     ISAVAKKRAP"
     gene            1653..2585
                     /locus_tag="VIX62_04940"
     CDS             1653..2585
                     /locus_tag="VIX62_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5859611.1"
                     /translation="MDPLAPDLPPPRAFPWWGVLPVLLPWLWFVARDHGAWTDPVAIA
                     LPAIAFVAFLAWAILTVFHRWVAAATALSLLAVCLVAIMGPRLPQRTAAPVRPIRIVS
                     DNVFNTSQWPGLAAQTMTSRGADVIVSVEMGRSYWEHLQEYTTAYPYTAIKSEQSILS
                     RWPVEALPLADGLPQDRIMRAALDVEGERVIVYVVHLFNPTHETTFDAQQRLLAQLVT
                     AMDAETDPVIVAGDLNLSDRSEGYRMLDDHFADAMRTGWWAGSTYRHGLWRALLLRID
                     QILVPLDWCTADAETFRVPGSDHMAVQSTVGPCF"
     gene            complement(2656..>3327)
                     /locus_tag="VIX62_04945"
     CDS             complement(2656..>3327)
                     /locus_tag="VIX62_04945"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5859612.1"
                     /translation="EDGEIDLKPEYTGFDLPLYDKNAPTNGEPQAVADALAGAVADDG
                     LVTYAISPANSTNVFVVLPDTASTNSLTDMSSLAPVAGQMTLGGPPDCPKADFCIPGL
                     EETYGIVFGEFKPLDFGGPKTVAAIDSGAVQVGELFSLDPTITDKGYVVLTDNENLQS
                     NGNFIPVVREEVASEELGALLDSVTTTLTDDNMREMVGQVLNDKRDVEDVASEYLTNA
                     GIISS"
BASE COUNT          510 a         1121 c         1108 g          588 t
ORIGIN      
        1 tcaccgcccc agtagtcgta cccgagctgc agcccgttgg gttcggtctg atcgtcgtcg
       61 agggcccaca tcaccggatg gccctcatgg atgtagaaga tgcctccggg cttcaggaag
      121 gcggccaccg accgtgccca cggcctgagg tcgggaagcc aactgagcgc cccgcggccc
      181 gtgtagacga tgtcgaaggt ctcgccatcg agcggaccgg gcaggtcgta gatgttcgac
      241 acgacgaacc gggcacgatc ggcgaggccc gtttcggtcg cgagttcgat ggcgaacgca
      301 atcgccggct cggagaagtc gacgccggtc acgtgcgagg cacccagacg agcccacgaa
      361 agggtgtcga gcccgaagtg acactggagg tggagcaagg tcttgccggc gacgtccccg
      421 acctcttcct gctcccacgg ctcgatgcgc acgtcgttcg ggtcgtcctg gaaccgttgg
      481 acgtcgtaga agtccccggt ggtgtggatc gccgtccacg cgtcccacaa ctttcggttg
      541 tccgcgatct cgcttgcgtg atcggtcatg gagcccgctt cttggccacg gcgctgatcg
      601 cttccatcac cgtgcggtga gcgaggatgg cggtgacctc ggcgttgtcg taaggcggag
      661 agacctccac cacgtccatg ccgaccaggt cgacggcacc cacgatctgg cgaaccgcgc
      721 gcagcagttc acgagccagc atgcccccgg actccggcgt gccggtgccc ggagccatgc
      781 cgggatcgac gacgtcgatg tcgacgctga ggtagatgca gtcgggaccg tcgagcgcct
      841 cggcgatcgc gtcggcgatc actgcttcgg agcctcggtc ttcgatctcg accatggtgt
      901 gccagcggag tccctgctcg cgcatccatt cccagttgct cttctcgggc cagtaaccgc
      961 gcaaacccac ctggacgaag ttgcggccgg ccacccagcc ctcttcgatc agccggcgca
     1021 tcggctcacc gtggccgtag aggttccccc attggtcgga gcccgtgtcg gcgtgagcgt
     1081 cgaagtggat cacacccacc gtccggggcc atacgtgacg cgccacgccg gccacgctcg
     1141 ggtaggtgat cgaatgatcg ccgcccaaca cgatcgggat cggccctgcg ctggccacgc
     1201 ggaacacctt ctcgtggatc actcgtagcc cgcgctcgaa ccgggtgggg acgatcggcg
     1261 cgtcgccggc gtcgacgacc gtcacgtgct cgtagggctc gacgtcgagc tggatcgacc
     1321 atgcgtagtc gctgctccag gcggtgggcg ccatgcggat cgcgcggggg ccgaaccggg
     1381 cccccgggcg cgacgaggtt ccctcgtcca acggggcgcc gacgatcgcg atatcggcgt
     1441 tcgcggcgcg caggctctcg tcatcggtgg cccacggcag cttctcgaac gacccacgcc
     1501 aatccgagta gtacgggaga tcggtggacg tgaattccga gaactcgtcc tcggagatca
     1561 gctcgctgtg ctccagccgg aagtcgggtg cgtcgctcat gggcgcgata ttgccacagg
     1621 gcggacacac cacgcgggta cgctccgggg ccatggatcc gctcgccccc gacctcccgc
     1681 caccgcgagc cttcccatgg tggggcgtgc ttccggtgct gttgccatgg ttgtggttcg
     1741 tcgcgcgcga tcacggcgcc tggaccgatc cggtcgccat cgccttgccg gcgatcgcgt
     1801 tcgtggcctt cctggcatgg gcgatcctga ccgtcttcca tcggtgggtc gcggcggcga
     1861 ccgcgttatc gctgctcgcg gtgtgcctgg tggcgatcat gggtccacga ttgccgcagc
     1921 gcacggccgc gcccgtgcgg ccgatccgca tcgtgtcgga caacgtgttc aacacgagcc
     1981 agtggcccgg cctcgccgcc cagacgatga cgagtcgcgg cgccgacgtc atcgtctccg
     2041 tcgagatggg ccgctcgtat tgggaacatc tgcaggagta cacgacggcc tacccctaca
     2101 ccgcgatcaa gagtgaacag agcatcctgt cgcgctggcc ggtggaagcg ctgccgctcg
     2161 cagacggcct gccccaggat cggatcatgc gggccgccct cgacgtcgag ggcgaacgcg
     2221 tcatcgtcta cgtggtgcac ctgttcaacc ccacgcacga gaccacgttc gacgcgcaac
     2281 aacgattgct cgctcagctg gtgacggcca tggacgccga gaccgatccc gtcatcgtcg
     2341 cgggtgacct caacctgtcc gatcgctcgg agggctaccg gatgctggac gaccacttcg
     2401 ccgacgcgat gcggacggga tggtgggcgg gcagcaccta ccggcacggc ctgtggcgag
     2461 ctctgctgct gcggatcgac cagatcctcg tgccgctcga ctggtgcacc gccgacgccg
     2521 agaccttccg ggttccgggt tcggatcaca tggcggtgca gtccaccgtc ggcccctgct
     2581 tctagccgta tccgaacacg ccgaagcccc aggccgacgt gggccgcggg ggcttcgatg
     2641 cgtctgcgtg gaacgctagg agctgatgat gccggcgttc gtcaggtact cgcttgcgac
     2701 gtcttcgacg tcacgcttgt cgttcagtac ctggcccacc atctcgcgca tgttgtcgtc
     2761 ggtgagggtg gtggtgacgc tgtccagcag cgcgcccagt tcctcgctgg caacctcctc
     2821 gcgcacgacc gggatgaagt tcccgttcga ctggaggttc tcgttgtcgg tgagtaccac
     2881 gtagcccttg tcggtgatcg tggggtcgag cgagaagagc tcgcccacct gtacggcgcc
     2941 gctatcgatc gcggcgaccg tcttgggtcc gccgaagtcg agcggcttga actcaccgaa
     3001 cacgatgccg taggtctcct ccaggcccgg gatgcagaag tcggccttcg ggcagtccgg
     3061 cggccctccc agcgtcatct gcccggccac gggggcgagg ctgctcatat cggtgagact
     3121 gttggtcgac gcggtgtccg gcagcaccac gaacacgttc gtgctgttcg ccggcgagat
     3181 cgcgtaggtc accaagccgt cgtcggccac ggcacccgcg agggcgtcgg ccacggcctg
     3241 aggctcaccg ttcgttgggg cgttcttgtc gtagagcggc agatcgaagc cggtgtattc
     3301 gggcttgaga tcgatctcgc cgtcctc
//
LOCUS       DATDBZ010000199         3201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2113690, whole genome shotgun sequence.
ACCESSION   DATDBZ010000199 DATDBZ010000000
VERSION     DATDBZ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113690"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VIX62_04950"
     CDS             <1..882
                     /locus_tag="VIX62_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5859613.1"
                     /translation="TVLVSETDREPFPVVTASRDGEVVHTFGRFAELPLIEARPTYAS
                     VGPKDLRIALFTPGGHEPGEPLPVLLDPYGGPHFGRVVRMQRMHLESQWFADQGFAVL
                     VADGRGTPYRGVAWDQAVHLNYLDAALEDQVEALHAAAERFPFLDLSRVAMRGWSFGG
                     YLVLGAMLRRGDVFHAGISGAPVSDPSYYDTHYTERDLGVPEEQPEAYRRTNVVKDAA
                     GLDGELMIIHGVVDDNVYVTHALQMSKALMEAGRRHAMIPLSGITHRPIDPAAAENMM
                     KIEVDFLRRSLGIADPA"
     gene            complement(944..2677)
                     /locus_tag="VIX62_04955"
     CDS             complement(944..2677)
                     /locus_tag="VIX62_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5859614.1"
                     /translation="MRGARNRIVAFAATMALVLPLWVGGGTAQGGVPSHPTRVLIVLF
                     DQMIPQYANQFDMPNYRELRNEGTNFRNAYLGYMASETVIAHNVITSGQLPKHMGWVD
                     EAYRDADNLLGKGAGAMHITGDLSSADFSTLINASYNGAGYPKLADYLQTADPGSKFI
                     AVGEKSYAVDSATAPNGAIGVRMSSRSSSSLFDSCRTTLGGRYRFPFGTNVPSYLSDY
                     CGRWYINSDAGNSYGTTTAFPSWLYPEDGNRFFPGTDASAVAGHTGGDTWVADAAIEM
                     MAREPWSGMFVTLGAIDKAAHMWGAQKDAATFMGACHTGDPVADGAAQTHVRCAAENA
                     DVQLGKMLDAVETLDASDGGETMVVLTADHGATYGEHFYGKDTAGAGDSNWYYAPTGV
                     WDAGSFIPATDPLYNQPSPSLQPLIATGNIQFSYQSSAIEAWLINHSTYKKKLGASAM
                     LKAPGVIASYWRDGGRYRLRGTNAMTLAEARWWNRHGQELVNTMAASNGPDVIGLLHD
                     KTTYSVLGDHGGAQESVQRVPMVFWSPSLAYSNNTYARFTTPDVMPTILRLMGIPFTE
                     PVDGVARRLDA"
     gene            2830..>3201
                     /locus_tag="VIX62_04960"
     CDS             2830..>3201
                     /locus_tag="VIX62_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY5859615.1"
                     /translation="MPIIVQKYGGTSVGSVERIQAVADRVVAAREAGNDVVVVVSAMG
                     HTTDELLAMASEIAPIPDPRELDMLLTAGERIAMSLLGIAINARGCRAASYTGSQAGI
                     MTDHEHGKARIVEIRPKRILEA"
BASE COUNT          487 a         1041 c         1118 g          555 t
ORIGIN      
        1 accgtcctgg tgtccgagac cgatcgggag ccgttcccgg tagtgaccgc gtcacgcgac
       61 ggcgaagtcg tccacacctt cggtcggttc gctgagctgc cgctgatcga ggcccggccc
      121 acgtatgcgt ccgtgggccc gaaggacctg cgcatcgcgc tgttcacgcc gggcggccac
      181 gagccggggg agccactccc ggtgctgctc gatccatacg ggggtccgca tttcggcagg
      241 gtcgtgcgga tgcagcggat gcacctggag tcccaatggt tcgccgacca ggggttcgcc
      301 gtgctggtgg ccgatggtcg cggcaccccg tatcgcgggg tggcctggga tcaggcggtg
      361 cacctgaact atctggacgc ggcgctcgag gaccaggtgg aggcgttgca tgccgccgcc
      421 gagcggttcc cgttcctgga tctcagccgg gttgcgatgc gcggatggtc cttcggcgga
      481 tatctggtgc tcggggcgat gttgcgccgc ggcgatgtgt tccacgcggg gatctcgggg
      541 gcgccggtgt cggatccctc gtactacgac acgcactaca ccgagcgcga cctcggggtg
      601 ccggaagagc aaccggaggc ctaccgtcgc acgaacgtcg tgaaggatgc tgcggggctg
      661 gacggtgagc tgatgatcat ccacggggtg gtcgacgaca acgtgtacgt cacgcatgcg
      721 ctgcagatgt ccaaggcgct catggaagcg ggccgccgtc acgcgatgat cccgctgagc
      781 ggcatcaccc accgcccgat cgatcccgcg gccgccgaga acatgatgaa gatcgaggtc
      841 gacttcctgc ggcgatcgct cggcatcgcc gatccggctt gaatcgcgcc gagcggtccg
      901 gaggccgaag cccccggacc gtgacggcga ggatcaggtg ttgctacgcg tcgagcctcc
      961 gcgccacccc gtccacgggc tcggtgaacg ggatccccat cagccgcagg atcgtcggca
     1021 tgacgtccgg tgtcgtgaac ctggcgtacg tgttgttgct gtacgccagg ctgggtgacc
     1081 agaacaccat cggcacgcgc tgcacgctct cctgcgcgcc gccgtggtcg cccaagacgc
     1141 tgtaggtggt cttgtcatgc agcagtccga tcacgtccgg cccgttggaa gcagccatcg
     1201 tgttcacgag ctcctggccg tgacggttcc accagcgggc ctcggccaga gtcatcgcgt
     1261 tggtgccccg caggcggtag cgcccgccgt cgcgccaata gctcgcgatc acccccgggg
     1321 ccttcagcat cgccgatgcg cccagcttct tcttgtaggt ggagtggttg atcagccacg
     1381 cctcgatcgc tgaggactga taggagaact ggatgttccc cgtggcgatc aacggctgca
     1441 ggctcggcga cggctggttg tagagcgggt cggtcgcagg gatgaacgag cccgcgtccc
     1501 agactccggt cggcgcgtag taccagttgc tgtcgccggc gccggcggtg tccttaccgt
     1561 agaagtgctc gccgtaggtc gcgccgtggt cggccgtcag cacgaccatg gtttcaccgc
     1621 cgtcgctcgc gtcgagcgtc tccacggcgt ccagcatctt gccgagctgc acgtcggcgt
     1681 tctcggcggc gcatcggacg tgcgtctggg ccgcaccgtc ggccacggga tcgcccgtgt
     1741 ggcacgcacc catgaaggtc gcggcgtcct tctgtgctcc ccacatgtgc gcggccttgt
     1801 cgatcgcacc cagcgtgacg aacatccctg accagggctc gcgggccatc atctcgatcg
     1861 cggcatcggc gacccacgtg tcaccaccgg tgtgcccggc gactgcggag gcgtcggtgc
     1921 cggggaagaa ccggttgccg tcttccggat acagccacga ggggaacgcc gtcgtggtgc
     1981 cgtagctgtt gccggcgtcg gagttgatgt accaacgtcc gcagtagtcg ctcaggtagc
     2041 tcggcacgtt cgtgccgaac gggaaccggt agcggccgcc gagcgtggtg cggcaggaat
     2101 cgaagagcga actcgaggac cggctcgaca tccgcacgcc gatggcgccg ttcggggcgg
     2161 tcgccgaatc caccgcgtag gacttctcgc cgacggcgat gaacttcgag ccgggatcgg
     2221 ccgtctggag atagtcggcg agcttcgggt agcccgcgcc gttgtatgag gcgttgatca
     2281 gcgtgctgaa gtcggcactg ctgaggtcgc cggtgatgtg catggcgccg gcgcctttgc
     2341 cgagcaggtt gtcggcgtcg cggtaggcct cgtcgaccca gcccatgtgc ttcgggagtt
     2401 gcccggaggt gatcacgttg tgggcgatca cggtctccga cgccatgtag cccaggtagg
     2461 cgttccggaa gttggtgccc tcgttcctga gctcgcggta gttcggcatg tcgaactggt
     2521 tcgcgtactg cgggatcatc tggtcgaaca gcacgatcaa cactcgggtc ggatgcgagg
     2581 gcacgccgcc ctgcgccgtg ccaccgccca cccacagcgg caggaccaac gccatggtgg
     2641 cggcgaaggc cacgatcctg ttcctggcac ccctcatgcg tttccccctc ccgtgcgttg
     2701 ctggatgatg ctcccgcccg tgggataggg caaatcgcgt agggcgtccg acggagagga
     2761 ccgcttgacg aggtggtgag ggtgccgccg ctagactcga ccacgtcccg accttcacct
     2821 ggagcgtccg tgcccatcat cgtgcagaag tacggcggta cctcggtcgg atccgtggag
     2881 cggatccagg ccgtggccga tcgcgtggtg gctgcacgcg aagccggcaa cgacgtggtc
     2941 gtcgtggtct cggccatggg gcacacgacc gacgagctgc tggcgatggc gtccgagatc
     3001 gccccgatcc ccgacccgcg ggagctcgac atgctgctca ccgcgggaga gcggatcgct
     3061 atgtccctcc tggggatcgc gatcaacgca cgagggtgcc gcgccgccag ctacacgggt
     3121 tcgcaggccg ggatcatgac cgatcacgag cacgggaagg ccaggatcgt cgagatccgg
     3181 ccgaagcgga tcctcgaagc g
//
LOCUS       DATDBZ010000200         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1907614, whole genome shotgun sequence.
ACCESSION   DATDBZ010000200 DATDBZ010000000
VERSION     DATDBZ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907614"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1933
                     /gene="kdpB"
                     /locus_tag="VIX62_04965"
     CDS             <1..1933
                     /gene="kdpB"
                     /locus_tag="VIX62_04965"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY5859616.1"
                     /translation="GSLLTTILWVRDIIAKPAGSPPLWFTGLVTVWLWFTVLFANFAE
                     AIAEGRGKAQAASLRKMRQDTQARKLTNGREETVSASTLRKGDRVVVEAGMLIPGDGD
                     VVEGIASVDESAITGESAPVIRESGGDRCAVTGGTKVLSDRIVVEISTNPGESFLDRM
                     IALVEGAARQKTPNEIALHILLVGLTLVFLFACVTLVPLALYSGIPLSVTVIVALLVC
                     LIPTTIGALLSAIGIAGMDRMVQRNVLAMSGRAVEAAGDVATLLLDKTGTITLGNRQA
                     AAFLTVPGVLERDLADAAVLSSLADETPEGRSIVALAQDHHGLEPRTLDGATLVPFTA
                     QTRMSGVDHDGRHIRKGAADSVRRWVEEVGGEVPGAMDTIAHGIAADGGTPLVVAESD
                     DRSGVVRVLGVIQLKDVVKAGMRERFDQLRAMGIRTVMITGDNPLTAKAIAEEAGVDD
                     FLAEATPEDKMALIRREQAGGRLVAMTGDGTNDAPALAQADVGVAMNTGTQAAKEAGN
                     MIDLDSNPTKLIEVVEVGKQLLITRGSLTTFSIANDVAKYFAIIPAMFLGVFPQLERL
                     NVMGLDSPHSAILSAVIFNALVIVALIPLALRGVRFRAAGAAEVLRRNLLIYGLGGLV
                     VPFVGIKAIDVIITTLGVS"
     gene            1933..>2087
                     /locus_tag="VIX62_04970"
     CDS             1933..>2087
                     /locus_tag="VIX62_04970"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HEY5859617.1"
                     /translation="MRAQLLAALRAVLVFTVLVGVAYPLAVTGVAQLAFGDQADGSLI
                     ERDGVVVG"
BASE COUNT          331 a          732 c          695 g          329 t
ORIGIN      
        1 tggaagcctt ctcaccacca tcctctgggt gcgcgacatc atcgcgaaac cggcaggctc
       61 ccctccactt tggttcacgg gactggtaac ggtgtggctc tggtttacgg tgctcttcgc
      121 caatttcgcg gaagcgatcg ccgaagggcg gggcaaggcg caggcggcgt cgctgcggaa
      181 aatgcgccag gacacgcagg cgcggaaact cacgaacggt cgcgaagaga cggtgtccgc
      241 atccaccctt cgcaaaggcg atagggtggt ggtcgaagcc ggcatgctga tccctggcga
      301 cggtgacgtg gtagagggta tcgcctcggt cgacgaatcg gccatcaccg gcgagtcggc
      361 accggtcatc cgcgaaagcg gtggtgatcg ctgcgcagtc accggaggaa cgaaagtgct
      421 ctccgatcgc atcgtggtcg aaatcagcac caacccgggg gagtcgttcc tcgaccggat
      481 gatcgcgctg gtcgagggcg ccgcccggca gaagacgccg aacgagatcg cgctgcacat
      541 cctgctggtc gggctgaccc tggtgttcct gttcgcgtgc gtgaccctgg tcccgctcgc
      601 gctctactcg gggatcccgc tgtcggtgac ggtgatcgtc gcgctgctgg tttgcctgat
      661 cccgaccacg atcggggcgc tgctctcggc gatcgggatc gccggcatgg accgcatggt
      721 gcagcggaac gtgctcgcca tgagcggtcg tgccgtcgaa gccgccggcg acgtcgccac
      781 gctgctcctc gacaagaccg gcaccatcac cctcgggaac cgccaggcgg ccgccttcct
      841 caccgtcccg ggggtgctcg agcgcgacct cgccgacgcc gccgtgctgt cgagcctcgc
      901 cgacgagacc cccgaagggc gatccatcgt ggccctggcc caggaccacc acggactgga
      961 gccgcgcacg ctcgacggcg cgacgctggt gccgttcacc gcccagaccc gcatgagcgg
     1021 cgtcgaccac gacggccgcc acatccgcaa gggcgcggcc gactcggtgc gacgctgggt
     1081 cgaggaggtg ggcggcgagg ttcccggcgc gatggacacg atcgcccacg ggatcgcggc
     1141 cgatggcgga acgccgctgg tggtcgccga gtccgacgac cgatccggtg tcgtgcgggt
     1201 cctcggtgtc atccagctca aggacgtcgt gaaggccggg atgcgcgaac ggttcgacca
     1261 gctgcgcgcc atgggcatcc gcaccgtcat gatcaccggg gacaaccccc tcaccgccaa
     1321 ggccatcgcc gaggaggcgg gcgtcgacga cttcctcgcc gaggcgaccc ccgaggacaa
     1381 gatggccctc atccgccgcg agcaagccgg agggcggctg gtcgccatga caggcgacgg
     1441 caccaacgac gctcccgccc tggcgcaggc cgacgtcggc gtggccatga acaccggcac
     1501 gcaggcggcc aaggaggcgg ggaacatgat cgacctcgac tcgaacccca ccaagctcat
     1561 cgaggtcgtc gaggtcggca agcagctgct catcacacgc ggctcgctga cgacgttctc
     1621 catcgccaac gacgtcgcca agtacttcgc catcatcccc gccatgttcc tcggggtctt
     1681 cccccagctc gagcggttga acgtgatggg cctcgactcg ccccactcag ccatcctctc
     1741 ggcggtgatc ttcaacgccc tcgtgatcgt ggcgctcatc cccctggcgt tgcggggtgt
     1801 gcggttcaga gccgccggcg ccgccgaggt gctccgccgc aacctcctca tctacgggct
     1861 cggcggcctc gtcgtcccgt tcgtgggcat caaggccatc gacgtgatca tcaccacact
     1921 gggagtgagc tgatgcgtgc acaactcctc gctgccctgc gagcggttct cgtcttcacc
     1981 gtgctggtcg gcgtcgccta ccccctggcc gtgaccggcg tcgcgcagct cgccttcggc
     2041 gaccaggccg acggctcgtt gatcgagcgc gacggcgtcg tcgtggg
//
LOCUS       DATDBZ010000201         3714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2113882, whole genome shotgun sequence.
ACCESSION   DATDBZ010000201 DATDBZ010000000
VERSION     DATDBZ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113882"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(456..1475)
                     /locus_tag="VIX62_04975"
     CDS             complement(456..1475)
                     /locus_tag="VIX62_04975"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY5859618.1"
                     /translation="MGTAWDNETAPIASADSSLPRYADLGGHDGAPPGSSWGLWGPED
                     RMGCWNLLTPERAIAASALVQRGAVFALNADMSVPDPPLFGRGGFRHEVTGELEGPGH
                     DDVLHNWNTQGSSQWDGLRHIRNPIHGWYGGHPDGFHGIDAWAARGLVGRAVLADVDR
                     WREAQGRPIRHDEPDPITGDELLATLEAQGTVPEVGDLLLVRTGWLGWYHGLDPDARA
                     SLAGGHQNTGLHPEEHTAEVLWDLHVAAVGADNPSLEIWPPGALHSAEERDAISADGS
                     RRPELFVHQRIIPMLGMSIGELFELDALADDCAVEGTYEGFFTSAPLRLRHGVASPPN
                     AIVVR"
     gene            1516..1932
                     /locus_tag="VIX62_04980"
     CDS             1516..1932
                     /locus_tag="VIX62_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEY5859619.1"
                     /translation="MSDPVATDEIDEPSDGDQIRLSLPARHEYARVARIAIAALAPRL
                     GFTYREVEDLRLAIDESLILLLGVDRPDERITIRFGTEHGHVELHASTGFEPEIDPDA
                     RARFEALAGDLVDDWRLSDDGRELRLTKRHRSATDD"
     gene            1990..2361
                     /locus_tag="VIX62_04985"
     CDS             1990..2361
                     /locus_tag="VIX62_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859620.1"
                     /translation="MNWSPFTKGAAVGAASVIVLIFMGLGVLAAVWIAAAGDEPPDPT
                     ATRHEAVGVVTTTEPRLCVEGTEPAQEGTPTPAWCGLAQPGYVAPNIAVGDAVVGAII
                     YVETDPGSGTAFSVWESLTPE"
     gene            complement(2385..2825)
                     /gene="dut"
                     /locus_tag="VIX62_04990"
     CDS             complement(2385..2825)
                     /gene="dut"
                     /locus_tag="VIX62_04990"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006721203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY5859621.1"
                     /translation="MIEVPILRLDPGLSVPSYARDGDAGLDLLAREDAVVAAGGGRAL
                     VPTGIAIAIPVGYAGFVLPRSGMALRHGITVANTPGLIDSGYRGELQVVLLNTDPDAD
                     FHVVRGNRIAQLVVQQVEEVGWIPVAELPTDSERGAGGFGHSGS"
     gene            2906..3517
                     /gene="orn"
                     /locus_tag="VIX62_04995"
     CDS             2906..3517
                     /gene="orn"
                     /locus_tag="VIX62_04995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY5859622.1"
                     /translation="MLAWMDLEMTGLDPGRHTIVEIATIITDDELNLVAEGPDLVVHA
                     TEAELAEMDTVVVNMHTRSGLLPAITASTMTLADAGAATLEFLREHIPEPRTVPLCGN
                     SIGTDRRFLAAYLPEIEEYLHYRSVDVSTIKELARRWNPTIVKGAPDKDTTHRALDDI
                     RESIEELRYYREHLFIPAEAASADPTPAIAVVVADGGSDGTSA"
     gene            3528..>3714
                     /locus_tag="VIX62_05000"
     CDS             3528..>3714
                     /locus_tag="VIX62_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859623.1"
                     /translation="MRGDNQGDHASGHSHAHAATSVREERQPALDDATEMAPGIIRIQ
                     LPISLPGLGHVNCYALPD"
BASE COUNT          584 a         1326 c         1260 g          544 t
ORIGIN      
        1 caccatcggg gatcgccgcg tcaggaggct cgaccgccgt gggggctgtg tcatcgggca
       61 ttggagctcc tccttgtcgc cggcggggcg ggacggtcgc gatcggtcgg cgcgagcatc
      121 aggccacctg tgcggcctgg cgttgcaagg tccacttgtc gacctccccg atggcgacca
      181 gccccgccgc gagcagcagg cacaccagcc actggccgcc ggtgagcggc accgtgtcga
      241 ggatccggtt gggtacagcg aggacgcgcg tcctcctgcg tgcccgtggt gctcaagtcg
      301 actccgtgga gcgctccacc ctgcatcccc ctccgcagct gcgatgcggc gtgatcgaga
      361 acataggccg acggcctccc ttcacgggcg gactctcctt acgcccaggt gaactcgagc
      421 tgaattcatc cggcgaggat gccggatgcg accgttcagc ggacgacgat ggcgttgggt
      481 gggctggcga ctccgtggcg cagcctcagc ggcgccgagg tgaagaagcc ttcgtaggtt
      541 ccctccaccg cgcagtcgtc ggccagggcg tcgagctcga acagctcgcc gatcgacatg
      601 ccgagcatcg ggatgatgcg ctggtggacg aacagctcgg gccggcgcga gccgtcggcg
      661 ctgatcgcgt cgcgctcctc ggccgagtgg agcgctccag gcggccagat ctccagggac
      721 gggttgtcgg caccgactgc cgcgacatgg agatcccaca gcacctcggc ggtgtgctct
      781 tcggggtgca ggcccgtgtt ctggtgccct ccggccagag aggcccgggc gtcgggatcg
      841 aggccgtggt accaaccgag ccatccggtg cggacgagga gcaggtcgcc gacctcgggc
      901 acggttccct gggcctcgag cgtggcgagg agctcgtccc cggtgatcgg gtcgggttcg
      961 tcgtggcgga tcggacggcc ctgtgcctcg cgccagcgat cgacgtcggc gaggaccgcc
     1021 cgcccgacca gaccacgcgc cgcccacgcg tcgatgccgt ggaagccgtc gggatggccg
     1081 ccataccacc cgtggatcgg attgcggatg tggcggagcc cgtcccactg cgagctgccc
     1141 tgggtgttcc agttgtggag cacatcgtcg tgacccggcc cctccaactc gccggtgacc
     1201 tcatgacgga accctccgcg accgaacagc ggcgggtcag gtacggacat gtcggcgttg
     1261 agggcgaaca ccgcacctcg ctgcacgagc gccgaggcgg cgatcgcccg ctcgggggtc
     1321 aacaagttcc agcaccccat ccggtcctcg ggaccccaca acccccatga cgatcccggc
     1381 ggcgcgccgt cgtgtccacc gaggtcggca tagcgcggca gcgagctgtc tgccgacgcg
     1441 atcggagcgg tctcgttatc ccatgcggtc cccatgcagc ggactctacg tccggcctca
     1501 atacgctgag cgacgatgtc cgacccggtg gccaccgacg agatcgacga gccctccgat
     1561 ggcgatcaga tccgcctctc gctgccggca cgccacgagt acgcccgcgt cgcccgcatc
     1621 gcgatcgccg cgctggcgcc gcgactcggg ttcacgtacc gggaggtgga ggatctccgc
     1681 ctcgccatcg acgagagcct gatccttctg ctcggcgtcg accgccccga cgagcgcatc
     1741 accatcaggt tcggcaccga gcacggccac gtggagctcc acgcctcgac gggtttcgag
     1801 cccgagatcg atccggatgc ccgcgcccgg ttcgaggcac tcgcgggtga tctggtcgat
     1861 gactggcgct tgtccgacga cgggcgcgag ctgcggctca ccaagcggca ccggtcggcc
     1921 accgacgact gatctgcgcg tcgcccgctc cgccacgttg ggaccgctcg gtcgggccac
     1981 actctggcga tgaactggtc accgttcacg aagggtgcag ccgtcggcgc agcctcggtc
     2041 atcgtcctca tcttcatggg actgggggtc ctggccgcgg tgtggatcgc cgcggcaggg
     2101 gacgaaccgc cggacccgac ggcgacgcgg cacgaggccg ttggcgtggt cacgacgacg
     2161 gaaccgcgcc tgtgcgtcga ggggaccgag cccgctcagg aggggactcc gaccccagcc
     2221 tggtgcggcc tggcacagcc gggctacgtc gcgccgaaca tcgccgtggg cgatgccgtc
     2281 gtgggcgcca tcatctatgt ggagactgat cctggctcag gcacggcgtt ctccgtctgg
     2341 gagtcgctga cgcccgaata ggtcgcaccg gatcgcctct cggctcagct gccgctgtgg
     2401 ccgaatcctc cagcaccgcg ctcggagtcg gtcggcagtt cggccacggg gatccagccg
     2461 acctcctcga cctgctgcac cacgagttgc gcgatgcggt tgccacgcac gacgtggaag
     2521 tccgcgtcgg ggtcggtgtt gaggagcacg acctggagct caccccggta gccggagtcg
     2581 atgagccccg gcgtgttcgc cacggtgatc ccgtgtcgca gagccatgcc actgcggggc
     2641 aggacgaacc cggcatagcc aacagggatc gcgatcgcga tgccagtggg caccagtgcg
     2701 cgacctccgc ccgctgcgac gacggcatcc tcacgggcga gcagatcgag ccccgcgtca
     2761 ccgtcacgag cgtaggacgg gacgctcaac ccagggtcga gccggaggat cggcacctcg
     2821 atcacagggc gacagtagcc acgccccgct ctgccctggg accgccgacg cccggggcag
     2881 ccgccgagtt cgtaggatcc ccacgatgct cgcctggatg gacctagaga tgaccggact
     2941 cgatccgggt cgccacacca tcgtggagat cgcgacgatc atcaccgacg acgagctgaa
     3001 cctcgtcgcg gaaggccccg acctcgtcgt gcacgccacc gaggcagagc tcgccgagat
     3061 ggacaccgtg gtcgtgaaca tgcacacgcg cagcgggttg ctcccagcga tcacggcatc
     3121 caccatgacg ttggcggacg cgggcgccgc gacgctcgag ttcctccgag agcacatccc
     3181 cgagccccgc acggtcccgc tctgcggcaa ctcgatcggc accgatcgcc gcttcctcgc
     3241 cgcgtacctc cccgagatcg aggagtacct ccactaccgg tcggtcgacg tctccaccat
     3301 caaggagctg gcccgccgct ggaacccgac catcgtgaag ggggcgcccg acaaggacac
     3361 gacccaccgg gcgctcgacg acatccgcga gagcatcgag gagctgcggt actaccgcga
     3421 gcacctgttc atccccgctg aggccgcctc ggcggatccc acgcctgcca tcgccgtggt
     3481 ggtcgcggat ggtgggtcgg acgggacgtc cgcctagcat cgacgcgatg aggggggaca
     3541 accaggggga ccacgcatcg gggcactcgc acgcccacgc cgccacgtct gtgcgcgagg
     3601 agcgtcagcc ggcactggac gacgccaccg agatggcacc ggggatcatc cgcatccagc
     3661 tgccgatcag cctgcctggc ctcggccacg tcaactgcta cgcccttccc gatc
//
LOCUS       DATDBZ010000202         4547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2114009, whole genome shotgun sequence.
ACCESSION   DATDBZ010000202 DATDBZ010000000
VERSION     DATDBZ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114009"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VIX62_05005"
     CDS             <1..538
                     /locus_tag="VIX62_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY5859624.1"
                     /translation="PVAVAMDTVALALVSLASISERRLYRLLDHQQSNGLPAFLVHES
                     GLNSGFMLVQYTAASLVSESKSLAHPASVDSIPSSAGQEDHVSMGMTSARHARDIVTN
                     AEHVLAMEALAAAQALDLREPLVPGAATQAVRDAIRATVPFFQADREFGPDIADAVAM
                     VRTGALVRAAEAAAGPLG"
     gene            complement(569..1810)
                     /gene="hutI"
                     /locus_tag="VIX62_05010"
     CDS             complement(569..1810)
                     /gene="hutI"
                     /locus_tag="VIX62_05010"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY5859625.1"
                     /translation="MTNRGAAVAAALATANGPLPVRGGRFEARVTEDAGIAWDETGTI
                     VYVGPADGLPWPATVGDRTLGCIIPGFVDCHAHLPFAGWRADEFEARLRGASYADLHG
                     EEGGIFRSARQLAAATDDEVLEFCRPILAEMVAHGTTALELKTGYGLSVEAELRQARL
                     ARRLAAEVAAQTCTVTLLAAHAVPHGVSREDWIRLACAELIPTASSEGLVDAVDIYVE
                     DIAFSLDDLSAVAAAAETAGLPLRVHADQLGPSGAAGAAVALRARSADHLNHAGPEDV
                     AALGHAERSAAVLLPASTFFLRAGAPPARALIDAGAAVAIATDCNPGTSPVSSMPETI
                     AMACTLYGLTPLEALTAATANPAWVLGLHDRLGTLEAGKRADLVLLEEASFAQVPYRP
                     GHDPVISTFIGGELAWDAVPS"
     gene            1876..2322
                     /locus_tag="VIX62_05015"
     CDS             1876..2322
                     /locus_tag="VIX62_05015"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5859626.1"
                     /translation="MQVSRSIVLPTTAEEAWGVLMDWERQADWMLDADRVTVVSDDRE
                     GVGVRLQVRTRLFGVPAFTEPMEVTGWEPPRELVIRHGSLVAGTGTWTLAPVDGGVRF
                     TWTEHISLAVPFVGDAAARLYRPVLRMLMGRAMEGLRRHVIAIGPR"
     regulatory      2383..2467
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2473..3129
                     /locus_tag="VIX62_05020"
     CDS             2473..3129
                     /locus_tag="VIX62_05020"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEY5859627.1"
                     /translation="MRTALVFCGGGRSRVAITVADEPLVIAADIGAVEALRLGFAVEL
                     LVGDMDSVPAAVVAEIEGAGGRIQRHPVDKDATDLDLALAAAASHGVERTLIVGGDGG
                     RMDQLLGNAMVMISSRFASMQMDAIFGRAQAHVIRDGRDLVGAPGETLSLFAVGGPAR
                     GVSASGVRWPLDGATLEPGSSLGISNEFIDVIARIEVEDGVVLAIRPGDLALDEQGTS
                     "
     gene            3126..4196
                     /locus_tag="VIX62_05025"
     CDS             3126..4196
                     /locus_tag="VIX62_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5859628.1"
                     /translation="MNVRVRLAAASAALSLIAIACTGEATDASSVPEEPVTITLLTHD
                     SFDVSKEVVDTFERSSGITLKIVPAGDAGQLVNRAILSAGNPEGDVLFGVDDNLFPKA
                     IEAGVFDTYRPAALDQVGPAIVLDPTDHVVPIDHGEVCLNIDRAWFADHRIEPPAGLE
                     DLTSPTYRGLTVVENPATSTPGLSFLLATVARFGDPGFETFWTQLRANDVRVVDGWEQ
                     AYYGGFSGAGGGEGDRPIVVSYASSPVAEVVFAEETLDEAPTAVVTDGCYRQVEFAGV
                     LAGTDHPAEAREVVDFLLSPDFQEDLPLRMFVYPVVEGTALPEVFERFGATVNDPLSM
                     PPEDVAEHRADWVQRWTDLMLA"
     gene            4172..>4547
                     /locus_tag="VIX62_05030"
     CDS             4172..>4547
                     /locus_tag="VIX62_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY5859629.1"
                     /translation="MDRPDARLRRVAVALALAAVPVAFLGLFFAYPLASILERGLGSG
                     ENTPSPLDVLTDPLIREVAWFTLWQAIASTVLTIAVALPASYVLGRYAFRGRNLVAAA
                     VTVPFVLPTVVVALAFLAVLPDG"
BASE COUNT          680 a         1502 c         1605 g          760 t
ORIGIN      
        1 gccggtggcc gtggcgatgg acaccgttgc gctcgccctg gtgtcgctgg cctcgatcag
       61 cgagcggcgg ctctaccgac tgctggacca ccagcagtcc aacggactcc cggcgttcct
      121 ggtgcacgag agcgggctca actcagggtt catgctggtg cagtacacgg ctgcgtcgtt
      181 ggtgagcgag tcgaagtcgt tggcgcaccc ggcgtcggtc gattcgatcc catccagcgc
      241 gggtcaggaa gatcacgtca gcatgggcat gacatcggca cgacacgccc gtgacatcgt
      301 cacgaatgcc gagcacgtcc tggcgatgga ggccctcgcc gctgcgcagg cgttggatct
      361 acgcgagccg ttggtgcccg gcgctgccac ccaggcggtg cgcgatgcga tccgagcgac
      421 ggtgccgttc ttccaggcag atcgggagtt cggtcccgac atcgccgatg ccgtcgcgat
      481 ggtgcgtact ggagccctgg tcagggcggc cgaggcggcg gcgggtccac tcggatagga
      541 cggtcgccct tcgctagtct gatatggact agctgggcac ggcgtcccac gccagttcgc
      601 cgccgatgaa ggtgctgatc accgggtcgt gcccggggcg ataggggacc tgagcgaatg
      661 aggcttcctc caagagcacc agatcggctc gcttccctgc ttccagcgtg ccgagtcgat
      721 catgcaggcc cagcacccat gcgggattcg cggtggcggc ggtcagtgcc tcgagcgggg
      781 tgagtccgta cagggtgcag gccatcgcga tcgtttcagg catcgaggac acgggcgagg
      841 taccggggtt gcagtcggtg gcgatcgcga ccgccgcccc ggcgtcgatc agtgcgcgtg
      901 cggggggcgc ccccgcccgc aggaagaacg tggaggcggg cagcagcacc gctgcggagc
      961 gctcagcgtg cccgagggcc gcgacgtcct cagggccggc atggttcagg tgatcggcgc
     1021 tcctggctct caatgcaacc gcggcccctg ctgcgccgct cggccccaac tggtcggcgt
     1081 gtacccgcag ggggagcccg gcggtctcgg cggcggcggc gaccgccgaa aggtcatcca
     1141 gcgagaaggc gatgtcctcc acgtagatgt ccaccgcgtc gacgagtccc tcggatgagg
     1201 ccgttgggat cagttcggcg cacgcgagac ggatccagtc ctcgcgcgag acgccgtgag
     1261 gaacggcgtg cgcggccagg agcgtcacgg tgcatgtctg tgcggccacc tcggccgcga
     1321 gtcgccgagc cagacgggcc tgtcgtagtt cggcttccac cgacaggccg tagcccgtct
     1381 tcagttccaa ggctgtggtc ccatgtgcca ccatctcggc caggatcggc cgacagaact
     1441 ccagcacttc gtcgtcggtc gcggcggcga gctgccgagc ggaacggaag atcccgcctt
     1501 cctcaccatg gaggtcggca taggaggcgc cccgaagacg cgcttcgaac tcgtcagctc
     1561 gccagcccgc gaacggaaga tgcgcgtggc agtcgacgaa gcccgggatg atgcagccga
     1621 gcgtgcgatc gccgacggtg gcgggccacg gcagcccgtc ggcgggaccc acgtacacga
     1681 tcgtgccggt ctcgtcccac gcgatgccgg cgtcctcagt gaccctggcc tcgaaccgcc
     1741 cgccacgaac gggaagcgga ccgttcgcgg tcgccaacgc cgcggcgacc gcggcacctc
     1801 ggttcgtcat cggcgaagcg tagctgcatc gcctcagcgg cgccgtcgtc ggcggacctg
     1861 cgagacactc cccacatgca ggtgtcccga tcgatcgtat tgcccaccac agcggaggag
     1921 gcatggggcg tcctgatgga ttgggaacga caggcggact ggatgctcga tgccgatcga
     1981 gtcaccgtgg tctcggacga ccgcgaagga gtgggtgtcc gactgcaggt tcggactcgg
     2041 ctcttcggcg tgcccgcctt caccgagccg atggaggtca ccggctggga gccgccccgg
     2101 gaactggtga tccgtcacgg aagcctggtc gccggcactg gcacgtggac cctggctccg
     2161 gtggacggag gcgtccggtt cacctggacc gaacacatca gcctcgcggt gccgttcgtc
     2221 ggtgatgcgg ctgcgcggct gtaccgaccg gtgctccgga tgctgatggg cagggccatg
     2281 gaaggcttgc gacggcacgt gatcgcgatc ggaccgcgct gacctgacaa cgccgccggt
     2341 ctgggcgcgg ccatccggca cctatactgg cgctgacatc cacggggagc tcgggcaacc
     2401 gggctgagag gccggctcac gcgccggcga cccgacgaac ctgaaccagg taatgctggc
     2461 ggagggagcc tcatgcgaac ggcgttggtg ttctgcggcg ggggtcggtc acgagtcgcg
     2521 atcaccgtcg ccgacgaacc cctcgtgatc gccgccgata tcggagcggt cgaagccctc
     2581 cgcctcgggt tcgcggtcga actcttggtg ggcgatatgg attcggtccc ggcggccgtg
     2641 gtcgccgaga tcgagggggc tgggggtcgc atccaacggc acccggtcga caaggacgcc
     2701 accgatctgg atctggcgct cgccgctgcg gcatcccatg gagtcgagcg aacgctgatc
     2761 gtgggtggcg acgggggccg catggaccag ctgctcggca acgccatggt catgatctcg
     2821 tcgaggttcg cgtcgatgca gatggacgcg atcttcgggc gcgcgcaggc gcacgtgatc
     2881 cgcgatggcc gagatctggt cggtgcgccg ggtgagacgt tgagtctgtt cgcggtgggg
     2941 ggacccgcgc gaggcgtcag tgcgagcggt gttcgatggc cgctggacgg cgcgacgctc
     3001 gaacccggct ccagcctcgg tatcagcaat gagttcatcg acgtcatagc ccgcatcgag
     3061 gtcgaagacg gtgtcgtgct cgcgatccgc cccggcgacc tcgcgctcga cgagcaaggc
     3121 acgtcgtgaa cgtccgcgtc cgcctcgcgg ccgcgtccgc agcgctgtct ctgatcgcga
     3181 tcgcgtgcac cggcgaggcg accgatgctt cgtcggtgcc cgaagagccc gtcaccatca
     3241 cgctgctcac ccacgattcg ttcgatgtga gcaaggaggt cgtggacacg ttcgaacgat
     3301 cgagcggtat caccctgaag atcgtcccgg caggcgacgc cggtcagttg gtgaaccggg
     3361 cgatcctgag cgccgggaac cccgagggtg atgtgctgtt cggcgtcgac gacaacctgt
     3421 tcccgaaggc gatcgaggcg ggcgtgttcg atacctatcg gccggctgcc ttggaccagg
     3481 tcggcccggc catcgtgctc gatcccaccg atcacgtcgt gccgatcgat cacggagagg
     3541 tgtgtctcaa catcgaccgc gcttggttcg ccgatcacag gatcgagcca cccgccggac
     3601 tcgaggacct gacgtcgccc acctatcgcg ggctcacggt ggtcgagaac ccagccacgt
     3661 caacgccggg gctctcgttc ctgctggcga cggtcgcgcg cttcggtgac ccgggtttcg
     3721 agacgttctg gacgcagctt cgcgcgaacg acgtccgtgt cgtcgacggg tgggaacagg
     3781 cgtactacgg cgggttctcg ggggccgggg gcggcgaggg cgaccgcccc atcgtggtgt
     3841 cgtacgcctc gagcccggtc gccgaggtgg tcttcgccga ggagacgttg gatgaggcgc
     3901 ccacggcggt cgtcaccgac ggctgctacc gccaggtgga gttcgcgggc gtcttggcgg
     3961 gcacggatca ccccgcggaa gctcgtgagg tcgtcgactt cctgctgtcg ccggacttcc
     4021 aggaagacct accccttcgg atgttcgtgt acccggtggt ggagggaacc gcgttgccgg
     4081 aagtcttcga gcgcttcggc gcgaccgtga acgacccgtt gtcgatgcca cccgaggacg
     4141 tcgccgaaca ccgcgcggat tgggttcaac ggtggaccga cctgatgctc gcctgaggcg
     4201 cgtcgctgtc gccctcgcgc tcgcggcggt ccccgtcgcg ttcctggggc tcttcttcgc
     4261 ctacccactg gcctcgatcc tggagcgagg tctcggctcg ggagagaaca ccccgtcacc
     4321 gctcgacgtc ctcaccgacc cactgatccg tgaggtggcc tggttcaccc tctggcaggc
     4381 aattgcctcc accgtgttga cgatcgcggt cgcgctccct gcgtcctacg tgctcggccg
     4441 ctacgcgttt cgcggtcgaa atctcgtcgc ggcggcggtg accgtgccgt tcgtccttcc
     4501 cacggtcgtg gtcgcgctcg catttctcgc cgtgttgccc gatggtc
//
LOCUS       DATDBZ010000203         8901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1068408, whole genome shotgun sequence.
ACCESSION   DATDBZ010000203 DATDBZ010000000
VERSION     DATDBZ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068408"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VIX62_05035"
     CDS             complement(<1..394)
                     /locus_tag="VIX62_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859630.1"
                     /translation="METTKCRACRSINHASSIRCLNCGAPLDPQVVDGSHPEGSPDFP
                     APPEGMPWVSMVKSADVRPKQTSPTAILFVTGGIILGIIAVVTALVMGDGGGFAVSRL
                     PTGFAVIAIVLAAFWLWMLIDAVASPMDS"
     gene            complement(449..1537)
                     /locus_tag="VIX62_05040"
     CDS             complement(449..1537)
                     /locus_tag="VIX62_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEY5859631.1"
                     /translation="MTHRITLIPGDGIGPEVVEAARRVIEATGTSVDWERQPMGAGAF
                     SRTGRTLPAQTVESIRATRVALKGPVETPVTSGLRSTNLELRKTLDLYANVRPCRLYP
                     GVPSVYDQVDLIVVRENTEGMYTGLEFEMGTVAVKELMRFVEDTTGRRIREDSGVSIK
                     TISRFGSERIVRFAFEEARRRGRTKVTASHKANIMKFTDGLFLEVARSVAQDYPDIAF
                     DDRIIDALCMQLIQAPERFDVLVMPNLYGDVAAELGAGLIGGVGVAPGAHFGGDDGVE
                     LALFEATHGTAPDLAGTNQANPLGVMLSGAMLLRHVGELDAGDRLEAAIAAVLADGTH
                     LPGDLRAPDDDRAPAGTFQMADAVIAQL"
     gene            1657..2772
                     /locus_tag="VIX62_05045"
     CDS             1657..2772
                     /locus_tag="VIX62_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY5859632.1"
                     /translation="MTEKGLREVIAAETVISDIDGEQGRLWYVGYDIADLAEHCTFEE
                     VVYLLHNRKLPNQDELDELNEFLVDNRELSPFLTELLSTLAQQASPMSMLRTSISAAS
                     AYDPDGWDDSPEAEYRKAMRLIAKTPALIAYYHRLRTGQDLIPPNPKLPHAANFLWML
                     LGDEPSQEDAEALDTTFTLYADHTMNASTFTARIIASTLSDMFSAITGAIGALKGPLH
                     GGANEEAMKAAEEVGSPENAAAYVAGRLERHEKIMGFGHAVYRTMDPRATVLKRLARE
                     SGERHEDVVWSEIFEAMQTTVYDQKQLWPNVDLYAAGVYHVLGIPTDLMTPIFALARM
                     AGWTAHVREQYADNKVIRPGSTYVGPRDQVWVPIDDR"
     gene            complement(2780..3697)
                     /locus_tag="VIX62_05050"
     CDS             complement(2780..3697)
                     /locus_tag="VIX62_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859633.1"
                     /translation="MQGAHEETRSYGPLVVLAGALVMAIAPFTAIAFGDSLFVQTHRD
                     LMWSLDAPIYAIAMVPPILLVVVAVLALAFPRSAVFRGALGMAAVMAVAVAAGTWVFV
                     LGVDGPQTRAAFVNAAITLTVAAIAITVGGLRSLQPRLSLAEPDRPITTVGPLLIIAG
                     AVCLAAAPFVFLAFGDDTNRYVIEQRHRLWIDLPQYLAGVLPAVAAFVFAVAALIRRR
                     SAVVATALLGVGLSTILLAVLSFWWLQGVVARPAKVAALRPSIVVLVVGAAVVLTGTV
                     VHFSVSRRLRSGATVSQPVTLRAVQGSSG"
     gene            complement(3797..5437)
                     /locus_tag="VIX62_05055"
     CDS             complement(3797..5437)
                     /locus_tag="VIX62_05055"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY5859634.1"
                     /translation="MTDAPPDLLIRNARAFTVDAARPWAEAIAVREGRIVWVGEDADA
                     SEGPDTEVIDAAGAAVLPGFIDTHNHARLGSNPLEVDLAGADSLDEVRARVRAHADAH
                     PEHTWIEGVGFNYSAMPDGRMPTAEDLDGLTGGRPAFLLTYDAHNAWLNREAMDVFAI
                     TRDTDTLPWGHVRKDPATGEPTGIVGDFAVMGISRAGQAALEPVLPGYERGLQYERTL
                     ESLDMATRFGITTILEPQNSPDDLWIFERARDEGRLRSRLIAAMFHPVGTTEAELQGF
                     DAARRRLGEDDRLRVGPIKLYIDDVIEPWTAAMLEPYANRPGERGDTFWTPREFAELV
                     TDLERRGWSCHVHGTGDRGLRVALDGFEAARLANGDVGARHGMVHTECLHADDVPRFG
                     ALGVAPIMQPRHCAPSIIADWRANVGPDRWRHAWAFRSLYETGARLSFSSDWNVAEMD
                     PLVGIYSAITRADLDGTGAWIPEETLDLETTLAAYTIGGAHVAFAEDRRGSITPGKQA
                     DLAVLSGDLFQAADADPRRILDLQVTHTVVDGEVVHRA"
     gene            complement(5434..6369)
                     /locus_tag="VIX62_05060"
     CDS             complement(5434..6369)
                     /locus_tag="VIX62_05060"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY5859635.1"
                     /translation="MSAVPAKVGGRLAALLRSGEFAVTGEIVPPRSANGDEVTRHARA
                     QVGYVDAANITDNPTASAHMSPVAGASFVARAGIEPTLQLTVRDRNRLAVTADLLGAW
                     AVGARNVLCLSGDPIHLGDHPDATVVRDIDVLQVVQLAKGLREHGTTMGGSEIADPPR
                     FLIGVADMPLADPYDPVRLEQKLDAGADVIWTQIAYDVEALAAWADILRPRGVFERAK
                     VLIGIVPLRTGNGARFMDEKLPGVRVPTEMIEALDTAGDDSAAVGVDLTVDVIDGIRS
                     IAGVSGIHLMGMGHDEAVRAVVERAGLFPRPTGAL"
     gene            complement(6366..7232)
                     /locus_tag="VIX62_05065"
     CDS             complement(6366..7232)
                     /locus_tag="VIX62_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY5859636.1"
                     /translation="MTARVLDGRAVSADIRSEVSERVRALSEKGVIPGLAAVLVGDDQ
                     ASRIYVGAKHKACADVGIRSEQVDLPAYVTEGELLATLRRLNRDPEIHGIIVQLPMPA
                     GLRELEVQRTVTPEKDVDGLHPWNVGMMLRGEPTFQPATPYGIVELLLRSGIELEGAE
                     VVVVGYGDLVGAPLSIMLAQDSIRGNATVTITHVKTRDLKAHTLKADILVAAAGVPQL
                     ITGDMVKPGATVIDVGVHRTADGLVGDVVYAEAAEVAGAITPVPGGVGPMTVAMLLVN
                     TVAAAEQQAFAR"
     gene            complement(7279..8829)
                     /gene="purH"
                     /locus_tag="VIX62_05070"
     CDS             complement(7279..8829)
                     /gene="purH"
                     /locus_tag="VIX62_05070"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY5859637.1"
                     /translation="MSDVQPIRRAILAPYDKTGLVAFATALAQRDVELVASGGTARVL
                     ADAGLAVTKVEDVTGSPEMLGGRVKTLHPSIHAGLLADRRNPDHVRQIEAFGIEPFDL
                     LVGGLYPFRETVASGADADAVIENIDIGGPAMMRAAAKNFESVTVVVDPARYEGILAE
                     IDGQGGVTRATRRTLAAAAFAHTAAYDAAVASWFAEHGDGGDDEALPGFVGLAYEKVG
                     DLRYGENPHQRGGLYREAATTALLGGASVLQGKDMSFNNWLDAYAAYELVAAMPEGSA
                     VIVKHNNPCGAAARGSLAESYRAAFACDMVSAFGGIVAFHGDVDADAAEAMAEVFTEV
                     VIAPSYTTAANAAFAQRPNLRLVAAPMLAPGGLDVRPLPGGALIQDRDTVSETRADWK
                     VVSSREPTAQEWADLELAWTIAWRVKSNTIVLVKDGATVGVGAGQMSRVDSSWIATRK
                     AGDRAQGAVLASDAFFPFPDALEVAADAGCGAVIHPGGSKGDEDVLAAAEARGMAVVL
                     TGTRHFRH"
BASE COUNT         1410 a         3205 c         2966 g         1320 t
ORIGIN      
        1 ctgagtccat cggactggcg accgcatcga tcagcatcca gagccagaac gccgccaaga
       61 cgatcgcgat cacggcgaac cccgtcggca gtcggctcac ggcgaaccca ccgccgtccc
      121 ccatcaccag tgccgtcacg accgcgatga tcccgaggat gatccccccg gtcacgaaga
      181 ggatggccgt cggcgatgtc tgcttcggac gcacgtccgc cgacttgacc atcgacaccc
      241 agggcatgcc ttctgggggg gctgggaagt ccggcgaacc ctcagggtgg ctgccgtcga
      301 ccacctgagg gtcgagcggg gcgccacagt tcaggcaacg gatcgagctc gcgtgattga
      361 tcgagcggca ggcccggcat ttcgtcgtct ccatcagaca gatggtcgac cttcacgacg
      421 ttgcccttga acaccacgat ccgaacgctc agagctgggc gatcacggcg tcggccatct
      481 ggaacgtgcc tgccggcgcc cgatcgtcat cgggcgcgcg caggtccccc ggcagatgcg
      541 tgccgtcggc gagcacggcc gcgatggccg cttccaaccg gtcgcccgcg tcgagctccc
      601 ccacgtgacg cagcagcatg gcgccgctca gcatcacgcc cagcgggttg gcctggttgg
      661 tgcccgcgag gtcgggcgcg gtgccgtgcg tggcttcgaa cagcgcgagc tcgacgccgt
      721 cgtcaccgcc gaagtgtgcg cccggcgcca cgccgacgcc gccgatcaga ccggcgccca
      781 gttcggcggc cacgtccccg tacaggttgg gcatcacgag gacgtcgaaa cgctcgggcg
      841 cctggatcag ctgcatgcac agcgcatcga tgatgcggtc atcgaacgcg atgtccgggt
      901 agtcctgggc cacgctccga gcgacctcca ggaataaccc gtcggtgaac ttcatgatgt
      961 tggccttgtg gctcgccgtg accttcgtgc gtccccgccg ccgggcttcc tcgaaggcga
     1021 accggacgat gcgctcactg ccgaaccggc tgatcgtctt gatcgagacg ccgctgtcct
     1081 cgcggatgcg cctgcccgtc gtgtcctcca cgaaccgcat gagttccttc accgccacgg
     1141 ttcccatctc gaactcgagg ccggtataca tgccctcggt gttctcgcgg accacgatca
     1201 ggtcgacctg gtcgtacacg ctgggcacgc ccggatagag ccggcacggg cggacgttcg
     1261 cgtacagatc gagcgtcttg cggagctcca ggttcgtgct ccgcaagccg ctggtcaccg
     1321 gtgtctcgac cggacccttc aacgccacgc gggtggcccg gatcgactcg acggtctgcg
     1381 ccggcagcgt ccgacccgtt cgcgagaacg ccccggcgcc catcggctga cgttcccagt
     1441 ccaccgaggt cccggtcgcc tcgatcacgc gccgcgccgc ttcgacgacc tcgggtccga
     1501 tgccgtctcc agggatcagc gtgatgcgat gggtcaacgc cagtaccttc ctgcgggtca
     1561 gccagcgatc tttggtccgt agaccggtcg cggactatcc tggcagatac gttcccgggt
     1621 gcccggggcg ttctgacgcg cgaaggatgt ggccaagtga ccgaaaaggg gcttcgagag
     1681 gtcatcgccg ctgagacggt gatctccgac atcgacggcg agcagggacg cctctggtac
     1741 gtcggctatg acatcgccga cctcgcagag cactgcacgt tcgaagaggt cgtctacctg
     1801 ctgcacaacc gcaagttgcc gaaccaggac gaactggacg agctgaacga gttcctggtg
     1861 gacaatcgcg aactcagtcc gttcctcacc gagctgctgt cgacgctggc ccagcaggcg
     1921 tcgcccatgt ccatgctgcg cacgtcgatc tcggcggcct cggcctacga ccccgacggg
     1981 tgggatgact cccccgaggc cgagtaccgc aaggcgatgc gcctgatcgc caagacccct
     2041 gcgttgatcg cgtattacca ccgcctccgc accgggcagg acctgatccc gcccaacccg
     2101 aagcttcccc acgccgcgaa cttcctgtgg atgctgctcg gcgacgagcc ctcgcaggaa
     2161 gacgccgaag cgctggatac cacgttcacc ctctacgccg accacacgat gaacgcctcg
     2221 acgttcaccg cgcgcatcat cgcctccacg ctgtccgaca tgttcagcgc catcaccggc
     2281 gcgatcggcg cgctcaaggg cccgctgcac ggcggcgcca acgaggaagc catgaaggcg
     2341 gccgaggagg tcggctcgcc ggagaacgcg gcagcctacg tcgccggccg gctggagcgg
     2401 cacgagaaga tcatggggtt cggccacgcc gtgtaccgga cgatggaccc gcgggcgacc
     2461 gtgctgaagc gtctcgcgcg cgagtcggga gagcgtcatg aggacgtggt ctggtcggag
     2521 atcttcgagg cgatgcagac caccgtgtac gaccagaagc agctgtggcc caacgtcgac
     2581 ctctacgccg ccggcgtcta ccacgtgctg ggcatcccca ccgatctgat gacgccgatc
     2641 ttcgcgctgg cgcgcatggc gggctggacc gcccacgtgc gcgagcagta cgccgacaac
     2701 aaggtgatcc gtcccggctc cacctacgtg gggccgcgcg atcaggtgtg ggtcccgatc
     2761 gacgaccgct aagtcagcct cacccggagg agccttgaac ggcccggagc gttacgggct
     2821 ggctcacggt cgcgccgctg cgcagacgcc ggctgacgga gaagtgcacc acggtcccgg
     2881 tcaagacgac cgcagcgccg acgaccagga cgacgatgct cggccgcagc gcagcgacct
     2941 tggccgggcg agcgaccacc ccttggagcc accagaacga gagcaccgcc agcaggatcg
     3001 tcgaaaggcc gacgcccagg agcgccgtcg ccacgacggc cgatcgccgg cggatcaacg
     3061 ccgccaccgc gaacacgaac gccgcgacgg cgggcaggac gccggccaag tactgcggga
     3121 ggtcgatcca gagccgatgc cgctgttcga tcacgtagcg gttcgtgtcg tcgccgaacg
     3181 ccaggaacac gaacggggcc gccgccaggc acaccgcgcc ggcgatgatc agcagcgggc
     3241 cgaccgtggt gatcggtcgg tccggttccg ccagggacag tcgcggctgg agtgaccgga
     3301 ggccgcccac cgtgatcgcg atggcggcta ccgtcagcgt gatcgcggcg ttcacgaacg
     3361 ccgctcgcgt ctgcggtccg tccacgccga gcacgaacac ccaggtgccg gccgccaccg
     3421 cgaccgccat gaccgccgcc atgcccaggg ctcctcggaa caccgccgag cgagggaacg
     3481 cgagcgcgag caccgcaacc acgacgagca ggatcggtgg caccatcgcg atggcgtaga
     3541 tcggcgcgtc gagggaccac atcaggtcac ggtgcgtctg gacgaacagc gaatcgccga
     3601 acgcgatcgc cgtgaacggc gcgatcgcca tgaccaacgc tccggccagc acgaccaacg
     3661 gcccgtagct ccgggtttcc tcatgcgccc cctgcatatg gagggaacgc tagtccgccg
     3721 ttcagcggct gagaaccgcc tttcggccta cccgagcacc acgcctgcag aacctttcgc
     3781 gaggccgccg ggacgatcag gcgcgatgga cgacctcgcc gtccaccacg gtgtgggtga
     3841 cctgcagatc caggatccgg cgcgggtcgg catcggcggc ctggaacaga tcgccgctca
     3901 gcaccgcgag atcggcctgt ttgcccgggg tgatcgatcc gcgtcgatcc tcggcgaacg
     3961 ccacatgggc gccgccgatc gtgtaggccg cgagcgtggt ctccaggtcg agggtctcct
     4021 cggggatcca cgcgccggtc ccgtccaggt ccgcacgtgt gatcgcgctg tagatcccca
     4081 cgagggggtc catctcggcg acgttccagt cgctggagaa cgacaacctc gcgcccgtct
     4141 cgtacaggga acggaacgcc caggcatgac gccagcggtc gggaccgacg ttcgcccgcc
     4201 agtcggcgat gatcgagggc gcgcagtggc gcggctgcat gatcggggcg acgccgagcg
     4261 cgccgaagcg aggtacgtcg tcggcgtgca ggcactcggt gtggaccatg ccgtgccgcg
     4321 cgccgacgtc gccgttcgcc aggcgggccg cttcgaagcc gtcgagggcc acgcgcaggc
     4381 cgcgatcgcc agtgccgtgc acatgacacg accacccgcg acgttccagg tcggtgacga
     4441 gttcggcgaa ctcgcgcggg gtccagaacg tgtcgccgcg ctctcccggg cggttcgcgt
     4501 acggctcgag catcgcggcg gtccacggtt cgatgacgtc gtcgatgtag agcttgatcg
     4561 ggccgacgcg gagccgatcg tcctcgccca ggcgccggcg cgccgcatcg aacccctgga
     4621 gctcggcctc ggtggtcccc accgggtgga acatcgcggc gatcaaccgc gatcggagcc
     4681 gtccttcgtc gcgggcacgc tcgaagatcc acaggtcgtc gggcgagttc tgcggctcca
     4741 ggatcgtggt gatgccgaac ctcgtcgcca tgtcgaggct ctccagcgtg cgttcgtact
     4801 gcagccctcg ttcgtagccg gggagcacgg gttcgagcgc cgcctggccc gctcggctga
     4861 tccccatcac ggcgaaatcc cccacgatgc cggtgggctc gccggtagcc ggatccttgc
     4921 gcacatgtcc ccacggcagc gtgtccgtgt cgcgcgtgat ggcgaacacg tccatggcct
     4981 cacggttgag ccaggcgttg tgggcgtcgt aggtcaggag gaacgccggc cgccctcctg
     5041 tcagcccgtc gaggtcctca gcggtgggca tgcggccgtc cggcatcgcc gagtagttga
     5101 accccacgcc ctcgatccag gtgtgctcgg gatgcgcgtc ggcgtgcgct cgtacgcgtg
     5161 ccctgacctc gtcgagggag tcggcgcccg ccagatccac ctcgagcgga ttcgagccga
     5221 gccgcgcgtg gttgtgcgtg tcgatgaacc cgggcaggac cgcggcgccc gcggcatcga
     5281 tcacctcggt gtcggggcct tcggatgcgt cagcgtcttc gcccacccac acgatccgcc
     5341 cctcgcgcac cgcgatcgcc tccgcccacg ggcgcgcggc atcgaccgtg aacgcccggg
     5401 cgttgcggat caggaggtcg ggcggagcgt cggtcacagc gccccggtgg gccgcgggaa
     5461 caagcccgcg cgctccacga cggcgcggac ggcctcgtcg tgacccatgc ccatcagatg
     5521 gatcccgctg acgccggcga tcgagcggat gccgtcgatc acgtcaacgg tgagatccac
     5581 tccgaccgcg gcgctgtcgt cgccagcggt atccagcgcc tcgatcatct ccgtgggcac
     5641 ccggaccccg gggagcttct cgtccatgaa ccgcgcgccg ttgcccgtcc gcagcggcac
     5701 gatcccgatc aggaccttcg cccgctcgaa cacgccacga gggcgaagga tgtcggccca
     5761 cgccgcgagc gcttcgacgt cgtaggcgat ctgggtccag atcacgtcgg cgccggcatc
     5821 gagcttctgc tccagccgga ccgggtcgta gggatcggcg agcggcatgt cggccacgcc
     5881 gatcaggaac cgtggaggat cggcgatctc ggatccgccc atcgtggtcc cgtgctcgcg
     5941 gagtcccttg gcaagctgca ccacctgcag cacgtcgatg tcccgcacca ccgtcgcgtc
     6001 ggggtgatcg cccaggtgga tcggatcacc cgacaggcac agcacgttcc gggcacccac
     6061 agcccacgca cccagcaggt ccgcggtgac ggccagccgg ttgcggtctc ggaccgtgag
     6121 ctgcagcgtg ggttcgatcc cggcccgcgc gacgaacgag gcgccggcga ccggcgacat
     6181 gtgcgcgctg gcagtggggt tgtccgtgat gttggccgca tccacgtagc ccacctgggc
     6241 gcgggcgtgg cgcgtgacct cgtcgccgtt ggccgagcgc ggcggaacga tctcaccggt
     6301 gacggcgaac tcgcccgatc gcagcagcgc cgccagccgt ccgccgacct tcgccgggac
     6361 cgcgctcacc gcgcgaacgc ctgctgctcc gcggcggcca ccgtgttcac caggagcatc
     6421 gccacggtca tcggtcccac gccgccgggc accggcgtga tcgcgcccgc gacctcggcg
     6481 gcttcggcgt acacgacatc acccaccagc ccgtcggcgg tgcggtgcac gccgacgtcg
     6541 atcacggtgg cgcccggctt caccatgtcg ccggtgatca actgaggcac ccccgcggcc
     6601 gccaccaaga tgtcggcctt cagcgtgtgg gccttcagat cgcgggtctt cacgtgggtg
     6661 atcgtcaccg tggcgttccc gcggatcgaa tcctgcgcca gcatgatcga cagcggcgcc
     6721 ccgacgagat cgccgtagcc gaccaccacg acctcggcac cttcaagctc gataccgctg
     6781 cgcagcaaca gctccacgat gccgtacggc gtggccggct ggaacgtggg ctcaccccgc
     6841 agcatcatgc ccacgttcca ggggtgcagg ccgtcgacgt ccttctcggg cgtgaccgtg
     6901 cgctggacct ccaactctcg aaggcccgcc ggcatgggca gctggacgat gatgccgtgg
     6961 atctccgggt cgcggttgag ccggcgcagg gtggcgagca gctcgccctc ggtgacgtag
     7021 gcgggcaggt cgacctgttc gctgcggatc ccgacgtcgg cacacgcctt gtgcttggcg
     7081 ccgacgtaga tgcgagaggc ctggtcgtcg cccacgagga cggcggccag gcccgggatc
     7141 acgcccttct cggagagcgc tcgtacccgc tcggatactt ccgagcggat gtctgcggaa
     7201 acggctctgc cgtccaagac ccgggcggtc acgaggcgca tcctacccgc cgcgcggccc
     7261 tgcgacgaaa ctcgtgggtc agtgtcggaa gtgccgggta ccggtgagca cgacggccat
     7321 gccacgggcc tcggcggccg ccagcacgtc ctcgtcgccc ttcgaaccgc cggggtggat
     7381 cacggcaccg cagccggcgt cggcggccac ctcgagcgcg tcggggaacg ggaagaacgc
     7441 gtcggatgcc aggaccgcgc cctgcgctcg gtcgccggcc ttccgtgtcg cgatccagga
     7501 cgaatccact cgcgacatct gccccgcccc cacgcccacg gtggcgccgt ccttgaccag
     7561 cacgatggtg ttcgacttca cacgccaggc gatcgtccac gcgagttcga ggtcggccca
     7621 ctcctgcgcg gtcggctcac gactcgacac gaccttccag tcggcgcggg tctcggacac
     7681 ggtatcgcgg tcctggatca gggcgccgcc gggcagcgga cgcacgtcga gcccgccggg
     7741 ggcgagcatc ggtgccgcca ccagtcgcag gttgggccgc tgcgcgaacg ccgcgttcgc
     7801 cgccgtggtg tacgacggcg cgatcaccac ctcggtgaac acctcggcca tggcctcggc
     7861 ggcatcggcg tccacatctc catggaaggc gacgatcccc ccgaacgcac tcaccatgtc
     7921 gcaggcgaac gccgcccgat aggactcagc gagcgatccc ctcgccgcag ccccgcaggg
     7981 attgttgtgc ttgacgatca cggccgagcc ctcgggcatg gccgcgacga gttcgtacgc
     8041 ggcgtaggcg tcgagccagt tgttgaacga catatccttg ccctgcagca cgctggcgcc
     8101 cccaagcagc gccgtggttg cggcttcgcg gtagaggcca ccgcgttgat gcgggttctc
     8161 gccgtaccgg aggtccccga ccttctcgta ggccagcccg acgaaccccg gcagcgcctc
     8221 atcgtcgcct ccgtccccgt gctcggcgaa ccagctggcg acggccgcgt cgtaggcggc
     8281 ggtatgcgcg aaggccgcag ccgccaacgt ccgtctggtg gcccgcgtca cgccaccctg
     8341 tccatcgatc tcggcgagga tgccctcgta tcgggccggg tccaccacga ccgtcacgga
     8401 ctcgaagttc ttcgcggccg cacgcatcat ggccgggccg ccgatgtcga tgttctcgat
     8461 caccgcgtcg gcgtcggctc cactcgccac ggtctcgcgg aagggataca gcccgccgac
     8521 cagcaggtcg aagggctcga tgccgaacgc ctcgatctga cggacgtggt cgggatttcg
     8581 acgatcggcg agcaaccccg cgtggatcga gggatgcaag gtcttgaccc tgccgcccag
     8641 catctcgggc gaaccggtga cgtcttcgac cttggtgacg gcgagcccgg cgtcggccag
     8701 cacccgagcg gtgccgcccg aggcgaccag ttcgacgtcg cgctgggcca acgccgtggc
     8761 gaacgccacc aggcctgtct tgtcatacgg tgccaggatc gcgcgtcgga tcggctgcac
     8821 gtcgctcatg gttcctcccc tgtgatgcgg accgctcgac cctccacgac caggcgaccc
     8881 tgcaacaacg cccgaacggc g
//
LOCUS       DATDBZ010000204        12873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2727645, whole genome shotgun sequence.
ACCESSION   DATDBZ010000204 DATDBZ010000000
VERSION     DATDBZ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2727645"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VIX62_05075"
     CDS             complement(<1..472)
                     /locus_tag="VIX62_05075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5859638.1"
                     /translation="MSEDPRIARPATRDDREVAVLSIVRAFAADPLVRWFFPDDGTYV
                     QRASTFFGLLFDGRVDDGNVLVAGDGAAVSMWDPPGGGSIAQAELDWRWDTEFEPGAG
                     PGERERLEAYEAAADTMMPPDASWYLGVLATDPDFQRQGLASAVIAPVLERADRD"
     gene            complement(469..750)
                     /locus_tag="VIX62_05080"
     CDS             complement(469..750)
                     /locus_tag="VIX62_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859639.1"
                     /translation="MTDWFHEDEDCWIAECMVCRTPMIVWRTHGLPDPEHEARLLAHL
                     ERVASERYGDGGFYVDGERRRIPDHWHAHARPAGGFFDPKSELYGAFDE"
     gene            complement(776..1249)
                     /locus_tag="VIX62_05085"
     CDS             complement(776..1249)
                     /locus_tag="VIX62_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY5859640.1"
                     /translation="MHRLLTLALVFGLVGSACQDQGRAEPSGNVVARATFSTQQEEAV
                     TGDLSVADSAEERRDGLMGVEALDEDGGMVFVFDGEQDGSFWMKDTLIPLSIAFWGED
                     GRLLDILEMAPCTADPCPTYSARAPYTHALEMNAHWFDDRGIEIGDRVELTIDSE"
     gene            1310..1384
                     /locus_tag="VIX62_05090"
     tRNA            1310..1384
                     /locus_tag="VIX62_05090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1342..1344,aa:Thr,seq:ggt)
     gene            1450..3504
                     /locus_tag="VIX62_05095"
     CDS             1450..3504
                     /locus_tag="VIX62_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY5859641.1"
                     /translation="MTTTNVAGEPDAGSKVRLVLLSFLMLFVELGLIRWAGAYNVYLS
                     FFTNFILLASFLGVGVGFLRAKGRFDLFRLAPAALAGLFVFLAVFPVQGGRVEGVLQV
                     VGGFGWPALPKWLSLSVVFGLSFLVMAAIASGVARVFARFESLEAYRLDILGSILGIV
                     GFSALAFLQASPLVWGLVVAVALVALSGRRRIVDLAAIVVVIGVLAVLSFAPLTHWSP
                     YQRITTSPRDADGIAIDVNGRPHQRIMSVSYMETEQAFRFEPYARSPGNPLTNVLIVG
                     AGSGNDVAIALAQGADHVDAVEIDPVLYALGRDLHPDRPYEDPRVDVYVEDGRAFIHD
                     TDTMYDLVIFAIPDSLAALAGQSSLRLESFLSTEEAVGEVRDHLAPGGAVAMYHYYLP
                     VALDRYGDTLDRTFGAPPCLDLSPGPGERPRTVLTASNDPGSLVCLDVWERPAAGIIP
                     ETDDFPFPYLVGRGIPTFYQLALLLILVGSLIAVRFAGGPLREMRSYVDLFFMGAAFL
                     LLETTNVVRFALWFGTTWFVNALVFGGILVSVWLAVEVTRRWPVRRPSLLYAPLFVSL
                     AIAWAVPIHALLDLAPAPRFVAAVVLGFTPVFLGNLIFANRFKDVGSSTIAFGTNLLG
                     AIVGGVLEYGALVVGYRSLLIGVAALYALALITGRAHLGSRLDTGKPTSIDPVTAAPR
                     AT"
     gene            3559..3633
                     /locus_tag="VIX62_05100"
     tRNA            3559..3633
                     /locus_tag="VIX62_05100"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3591..3593,aa:Met,seq:cat)
     gene            3736..3900
                     /gene="rpmG"
                     /locus_tag="VIX62_05105"
     CDS             3736..3900
                     /gene="rpmG"
                     /locus_tag="VIX62_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY5859642.1"
                     /translation="MAKADNRPKITLACTDCKRRNYNTVKNKVNDRDRIELKKYCRWC
                     RKHTLHRETR"
     gene            complement(3897..4661)
                     /locus_tag="VIX62_05110"
     CDS             complement(3897..4661)
                     /locus_tag="VIX62_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5859643.1"
                     /translation="MPKTGHEGPITVVIADDHRSFGEALEVALDKEQDLRVIEVVTDG
                     EAAVETASKEQPDIVLMDLQMPGVDGIEATRRIHDESTGTMVIILSGQNDDISLARAI
                     QAGARGYLRKTEAVGGLADAIRRAYRGEPLHTSAEVEESLRKLRRRRASDGNLAQRID
                     RLTPRETEILQRMADGDPPEKISRELGMSKHTLRTHTQNVLTKLGVHSKLDAIVAAIR
                     YGKISTVDVSPLVETDDLDEDTPGSAIEGPGEPDAT"
     gene            4749..5135
                     /locus_tag="VIX62_05115"
     CDS             4749..5135
                     /locus_tag="VIX62_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5859644.1"
                     /translation="MNPAAEGTTYPDMPFVVAPERVAGFREVFGQGQGVPPTFLTAAE
                     FTVLPTIVADPRLDVDFTRVVHGSQEYTFARAPREGERLTIKARLESVRVRAGTGFVT
                     ITIDLVDEAGDHVATCRSSMVERGDV"
     gene            5135..5557
                     /locus_tag="VIX62_05120"
     CDS             5135..5557
                     /locus_tag="VIX62_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY5859645.1"
                     /translation="MRAFEDVAVGDELPALHRVVTAEDMKAYADVSGDQNPLHQDEAF
                     ARSVGFDGIIAHGMFTMGHMAACIIGWSGGDPATVLQLSAQFRAPVFMGERIDAGGRV
                     RLLDAEARTATLETWVSVERDGTTEWPIKRGAATVRLA"
     gene            complement(5561..7141)
                     /locus_tag="VIX62_05125"
     CDS             complement(5561..7141)
                     /locus_tag="VIX62_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859646.1"
                     /translation="MDEYGPTASPQHAGASRRLLRRLEQYEDEPRMTPGEMRIAAAVE
                     RTVDERLADGLLAIEEQATALMREIAGEIWRSAGADARPEQERIVSLLSRDQTIKSLL
                     NSSDERFQSLALRSARLEDSLTDLTESGRATRASMEASVAAIREIADSPTLHGVEGVR
                     SQLEQVEHHIAAAFEHFDERDRALTEGVLGQIKAHGELVARETTRLVEVMQQYVQTGA
                     EAMAILATRIEEQADAFATQDLTISEHVGDMVAVRIRPVAEQLDLLAEKVGLHGRDQD
                     QVRAAIERLVEARIMGLAQLIRSDSEAIRRVVEERSEDQVEALREAVDWRMAAFAERI
                     DEQLERAGDQVATRASEAAERAIADSLGQTIERMSAGVGAIDGLDSMMAEGQAATEER
                     LREHIDDRMTAIARLIRSDNQILAESFKASTAAPAETVDPQLLRQTMLAIKELQAGLA
                     NDVRTAVDRRFESINDQLHKESQSTTESMVKVAEVLGDKIDRLSVRVDEGYGNDLQIV
                     IDRMGDAIQAMSSVRRTA"
     gene            complement(7255..8913)
                     /locus_tag="VIX62_05130"
     CDS             complement(7255..8913)
                     /locus_tag="VIX62_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY5859647.1"
                     /translation="MPARRPQLAELGLFLLVVALPLAFTPFSTSPFGDPKLVILVGGS
                     LALWGSGLPLDRRIGALAALWSVFTALSALTGVEPIRSLAARTDGEGGGLMLVLSCAV
                     IVVTGVGVDADLRERSRRWFVVASCIVGALGLVFRFAPDVLTDTLGLRTLSFVGATMG
                     NQLFAAALLAAGIAAALVPGKRLRNQVLVVAFLALGAASFGERSSLILPAVAVIVTLA
                     RTRMPWRASFALAATVGVVLLGWQIAEPVLPGRETGAGAALAPLQGEATDVDRAVMWR
                     VTSGSIGEHPLVGWGPGTTQVAYLTSATPADIAEATRGWADAHNLFLETTVTSGVLGL
                     IPLVALLLLLVGRAWRAGPERAWSLGAAAALGAYALVEPLNLVLTPLLFLFAAMAAGL
                     RPREPAADERTPRGVRLAVGIVLSLSLVVSLTMIAGATFERWGRHYGEEWAYRAALRV
                     QPWRLTSTEQLALQLAAQGRAGDEQAGAEAKRIIAEGVERFPWDVNVRVWAADVDTLL
                     NDPAGSRAWRRQHVERFPADETVLTDDGPDDGFTLPGEEPVTGG"
     gene            9088..9161
                     /locus_tag="VIX62_05135"
     tRNA            9088..9161
                     /locus_tag="VIX62_05135"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9122..9124,aa:Trp,seq:cca)
     gene            9173..9478
                     /gene="secE"
                     /locus_tag="VIX62_05140"
     CDS             9173..9478
                     /gene="secE"
                     /locus_tag="VIX62_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY5859648.1"
                     /translation="MNRQMKRMQERAERQQKRAGVDRQAAPAATTRRAQTQEKRKRTG
                     IRQFLKEVRQELRKVDWPNRRELVSYTVVVLATIVVMTAFVATLDYVFLKGVVKIFG"
     gene            9508..10638
                     /gene="nusG"
                     /locus_tag="VIX62_05145"
     CDS             9508..10638
                     /gene="nusG"
                     /locus_tag="VIX62_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY5859649.1"
                     /translation="MEDLEQSNDTPAEDALEAAEELSELADDAAAEALTEGVVEAAEV
                     EAVADATAAAVLDEAADEAEALSEAGDDEAAEEVLEQGIVDAVEIETVGEAVADEVLA
                     EAVEEAAEIEEAADDVIEELVVEAVVAEAVEEEAELEIGETEYLEMEAAQGDGELPIA
                     TESVAEAAVEPVVEPEPAAEPKAAEPEPEPEPKSDDPFKGPGDWYVVHTYAGYENKVK
                     TNLESRIHTMQMEGKIFNVHIPMEDVMEIKGGKKQVVQKKVFPGYLLVKMVYDNDSWY
                     VVRNTPGVTGFVSAGTGTKPTPLSRREVEKILTVKKEEAKPQVRLGFDEGDVVRIISG
                     PFADFNGTISEINADQAKLKVLVNIFDRETPVELSFEQVSKV"
     gene            10692..11120
                     /gene="rplK"
                     /locus_tag="VIX62_05150"
     CDS             10692..11120
                     /gene="rplK"
                     /locus_tag="VIX62_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY5859650.1"
                     /translation="MAPKKVLASVKLQIKAGQATPAPPVGTALGQHGVNIMEFCKQYN
                     EATQQFSGQVIPVELTIYEDRSFSFITKQPPAAELIKQAAGVEKGSAEPNRDKVARIS
                     PSQVREIAERKMADLNANDLDNAMRIIEGTARSMGVEVDA"
     gene            11117..11833
                     /gene="rplA"
                     /locus_tag="VIX62_05155"
     CDS             11117..11833
                     /gene="rplA"
                     /locus_tag="VIX62_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY5859651.1"
                     /translation="MSHGKRYREAAGNVDHEHEYQPVEAIKLLKAQPDAKFDETIEIA
                     FRLGLDTRKADQSIRGTVSLPNGTGKSVRVGVFATGEKAREAYEAGADVVGGEELVEQ
                     VMKGEIDFDAAVATPDMMASVGKAGRVLGPRGLMPNPKTGTVTMDIAKAVGDIKGGKV
                     EYRADRTGNVHMVIGKKSFGEQQLLENYLAVVDELLRAKPSSAKGRYIESLAVSSTMG
                     PSVRIDPAHPKEIEMAATSV"
     gene            11960..>12873
                     /locus_tag="VIX62_05160"
     CDS             11960..>12873
                     /locus_tag="VIX62_05160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5859652.1"
                     /translation="MAYPRVLHVIGSLEPGGTERQLVGFIQRSGEPGRHHVVVCSPGG
                     ELEKELPRSPDQIGPLKREFLGVPSGIRTVAELHRLISRIEPDIVHTHLGYPQVLASI
                     AVPRGVPIIASRRGRTPYLESSGFGRMLVGVANRRTRLLLTNSHDLARRAASERWTPP
                     SRVIPNGVDLDRFTPAPFPETGSPTVVMLARMRAEKRHDRFLRVFRRVVDQLPDARAI
                     LVGDGPCRDDVARLVGLMGLSERVDLVGGCDDPRSQLARSHVVTLTSDHEGFPNALLE
                     GMAMGRPIVATDVGGVAELVGDQAGITVP"
BASE COUNT         2079 a         4340 c         4384 g         2070 t
ORIGIN      
        1 catcgcgatc ggcacgctcg agcaccggcg cgatcactgc gctggccagt ccctgccgtt
       61 ggaagtccgg gtcggtggcc aggacgccga ggtaccaact cgcgtctggg ggcatcatcg
      121 tgtccgcggc ggcttcgtac gcctcgagtc gctcccgttc acccggcccg gcacccggtt
      181 cgaactcggt atcccatcgc cagtcgagct cggcctgggc gatcgatccg cctcccgggg
      241 gatcccacat cgacacggcg gcgccatcgc ccgccacgag gacgttcccg tcgtccacgc
      301 gaccgtcgaa cagcaatccg aagaacgtcg aggcccgttg cacgtaggtc ccgtcgtcgg
      361 ggaagaacca ccgcaccagc ggatcggccg cgaacgctcg gacgatcgag agcaccgcca
      421 cctcgcggtc gtctcgcgtc gccggccggg cgatgcgtgg gtcctcgctc actcgtcgaa
      481 cgccccgtag agctcgctct tggggtcgaa gaacccgccg gccggacgcg cgtgcgcgtg
      541 ccagtggtcg gggatccggc gacgctcgcc gtccacgtag aagccgccgt cgccgtagcg
      601 ttcagacgcc acccgctcca ggtgtgccaa gagccgggcc tcgtgctcag ggtctggcag
      661 accgtgggtt cgccacacga tcatcggcgt gcggcacacc atgcattcgg cgatccagca
      721 gtcctcgtcc tcgtggaacc agtcggtcac gcgctcagcg gaacacagga gacactcact
      781 cgctgtcgat ggtgagctca acgcgatctc cgatctcgat gccgcggtcg tcgaaccagt
      841 gggcgttcat ctcgagcgcg tgggtgtagg gcgctcgtgc cgagtacgtg ggacaggggt
      901 cggcggtgca cggtgccatc tcgaggatat cgaggaggcg cccatcctcc ccccagaacg
      961 cgatcgacag cgggatcagc gtgtccttca tccagaatga tccgtcctgt tcaccgtcga
     1021 acacgaacac catgccgccg tcctcatcca gcgcctccac gcccatgagc ccgtcgcgac
     1081 gctcctcggc ggaatccgcg acgctgaggt caccggtaac cgcctcttcc tgctgggttg
     1141 agaacgtggc ccgagccacg acgttgccgg agggctcggc ccggccctgg tcctggcacg
     1201 ccgagccgac caggccgaag accagcgcga gtgtgaggag acggtgcacc catccatggt
     1261 cgcacgcccc tcgagtggct cggccgcgca ttgctatcat caggcttccg ccggagtagc
     1321 tcaggggtag agcaacgcca tggtaaggcg taggtccggg gttcaaatcc ccgctccggc
     1381 tccaaggccg tccgcaccgc cctccctgtt agggtcgacc gctacgccgt cggaccccgt
     1441 ggagcgcagt tgaccaccac caacgttgca ggcgaacccg acgcgggatc caaggttcgc
     1501 ctggtcctcc tgagtttcct gatgctgttc gtggaactgg gcctgatccg atgggccggc
     1561 gcctacaacg tctacctgtc gttcttcacg aacttcatcc tgctggcgag cttcctgggt
     1621 gtgggtgtgg ggttcttgcg cgcgaaggga cgcttcgacc tgttccggct ggcaccagcg
     1681 gccctggcgg ggctgttcgt gttcctcgcg gtgttcccgg tgcagggagg ccgtgtcgaa
     1741 ggcgtcctcc aggtggtggg cgggttcgga tggcccgctc tgccgaagtg gctctcgctg
     1801 tcggtggtgt tcggcctgtc gttcctggtg atggcggcga tcgcgtcggg cgtggcgcgg
     1861 gtgttcgcgc ggttcgagtc cctcgaggcc taccggctgg acatcctggg cagcatcctg
     1921 gggatcgtgg ggttctcggc gctcgccttc ctccaggcct cgccgttggt gtgggggctc
     1981 gtcgtcgcgg tcgcgttggt ggcactgagc ggccggcgcc ggatcgtcga cctcgcggcc
     2041 atcgtggtgg tgatcggcgt gctcgcggta ctgtcgttcg cgccgctgac gcattggtcg
     2101 ccctatcaac ggatcaccac ctcgccgcgg gacgccgacg gcatcgcgat cgacgtcaac
     2161 ggccgcccgc atcaacggat catgtcggtg tcctacatgg agaccgagca ggcgttccgt
     2221 ttcgagccgt acgcacgctc gcccgggaac ccgctcacga acgtgttgat cgtcggggcg
     2281 ggcagcggga acgacgtcgc gatcgccctc gcgcagggag cggaccacgt cgacgccgtc
     2341 gagatcgacc ccgtgctgta tgcgctgggt cgcgacctgc accccgatcg tccgtacgag
     2401 gacccgaggg tggacgtcta cgtcgaggac ggtcgtgcgt tcatccatga caccgacacg
     2461 atgtacgacc tggtgatctt cgcgatcccg gattcactcg ccgcgctcgc cggccagtcg
     2521 tcgctgcgcc tcgagagctt cctgtccacc gaggaggcgg tcggggaggt gcgcgaccac
     2581 ctcgcgcccg gtggagcggt ggcgatgtac cactattacc tgccggtcgc tctcgatcgc
     2641 tacggcgaca cactcgaccg aacgttcggt gcgccgccct gcctcgacct gagccccggc
     2701 ccgggcgagc gcccgcgaac ggtgctgacc gcgagcaacg atcccggcag cctggtgtgt
     2761 ctggacgtat gggaacgacc ggccgccggg atcatcccgg agaccgacga tttccccttc
     2821 ccgtacctgg tggggcgcgg gatcccgacc ttctaccagc tggcgctgct gctgatcctg
     2881 gtgggttcgc tcatcgccgt tcggttcgcg ggaggaccgc tccgtgagat gcgctcctac
     2941 gtcgacctgt tcttcatggg ggcggcgttc ctgctgctgg agaccacgaa cgtcgtccgg
     3001 ttcgcgctct ggttcgggac cacgtggttc gtgaacgcgc tggtcttcgg cggcatcctg
     3061 gtgtcggtct ggctcgcggt cgaggtgacc cgccggtggc cggtgcggcg tccgagcctg
     3121 ctgtacgcgc cgctcttcgt gagcctcgcg atcgcatggg cggtgccgat ccatgccctg
     3181 ttggacctcg ctcccgcacc gcggttcgta gccgccgtcg tgctgggctt cacgccggtg
     3241 ttcctgggca acctgatctt cgccaaccga ttcaaggacg tgggttcctc gacgatcgcc
     3301 ttcggcacga acctgctggg ggcgatcgtg ggcggcgtcc tcgagtacgg cgcgctcgtc
     3361 gtggggtacc ggtcgctgtt gatcggcgtg gcggcgctct acgcgctggc gctcatcacg
     3421 ggccgggcac acttggggag ccggctcgat accgggaaac ccacttcgat cgatcccgtg
     3481 accgcggcac cacgggcgac ctgatctcag gtgtgccggg cgcgcatccg cccgggtaaa
     3541 ctcaacgttc gcatctaggg cggcgtagct caggggcaga gcaagcggct cataatcgct
     3601 gtgtcggtgg ttcgagtcca cccgccgcta ccagcccggt tgttggcccg caccgtcgga
     3661 accatgcacg gagggatcgg aacgcacgat cgtcgttgag gacgttgacc gagatcaccc
     3721 aaggaacagg acaccatggc caaggcagat aaccgcccga agatcacgct cgcgtgcacg
     3781 gactgcaagc ggcgcaacta caacacggtc aagaacaagg tgaacgaccg tgaccgcatc
     3841 gagctgaaga agtactgccg ctggtgcagg aagcacaccc tgcaccgcga aacgcgctag
     3901 gtcgcgtccg gctcgcccgg tccctcgatc gccgatcccg gcgtgtcctc gtcgaggtcg
     3961 tcggtctcca cgagcggcga gacgtcgacg gtgctgatct tgccgtatcg gatggcggcg
     4021 acgatcgcat ccagcttcga gtgcaccccc aacttcgtga gcacgttctg cgtgtgcgtg
     4081 cgaagggtgt gtttgctcat ccccagctcg cgggagatct tctccggcgg gtccccgtcg
     4141 gccatgcgct gaaggatctc ggtctcgcgc ggcgtgagcc ggtcgatccg ctgcgcgagg
     4201 ttgccgtcgg aggcgcgccg tcgacgcagc ttcctcaggg actcctcgac ctcggcgctg
     4261 gtgtggaggg gctcgccccg gtaggcgcgc ctgatcgcgt cggccaggcc gcccacggcc
     4321 tcggtcttgc gcaggtaccc gcgcgctcct gcctggatgg cgcgggccag tgagatgtcg
     4381 tcgttctgtc ccgacaggat gatcaccatg gtgcccgtgg actcgtcgtg gatgcggcgc
     4441 gtcgcctcga tcccgtccac gcccggcatc tgcaggtcca tgagcacgat gtcgggctgt
     4501 tccttcgagg cggtctccac cgcggcctcg ccgtcggtga cgacctcgat cacgcggagg
     4561 tcctgctcct tgtcgagcgc cacctccaac gcctcgccga acgaccggtg gtcgtcggcg
     4621 atcacgacgg tgatgggccc ttcatgtccc gtcttcggca tacggcgcga atcgtaggcc
     4681 ctgacctggg atgtttcacg cacctggagc aagttgctgg ggggcccggt cacggctacc
     4741 gtgcgcgcgt gaacccggcc gccgaaggca ccacctaccc ggacatgcca ttcgtcgtcg
     4801 cgccggaacg ggtcgccggg ttccgcgagg tgttcggcca aggccaaggg gtgccgccca
     4861 cgttcctcac ggcggccgaa ttcacggtgc tccccacgat cgtggccgac ccccggctgg
     4921 acgtcgattt cacacgcgtg gtgcacgggt cgcaggagta caccttcgcc cgcgcgccac
     4981 gggagggtga gcggctcacg atcaaggccc ggctcgagtc cgtccgggtg cgcgccggca
     5041 ccggcttcgt cacgatcacc atcgacctgg tggacgaggc tggcgaccac gtcgccacct
     5101 gccggtcgtc catggtcgaa cggggcgacg tctgatgagg gccttcgagg acgtggcggt
     5161 gggggacgag ctcccggcgc tccaccgcgt ggtgacggcc gaggacatga aggcctatgc
     5221 cgacgtgagc ggcgatcaga atccgctgca ccaggacgag gcgttcgccc gttcggtggg
     5281 cttcgacggc atcatcgccc acggcatgtt cacgatgggc cacatggcgg catgcatcat
     5341 cgggtggtcg ggcggcgacc ccgcgaccgt gctgcagctc tcggcgcagt tccgggcccc
     5401 cgtgttcatg ggtgagcgga tcgatgccgg cggccgtgtg cgcctgctcg atgccgaggc
     5461 gcgcaccgcc acgctggaga cctgggtcag cgtggagcgc gacggcacca ccgaatggcc
     5521 gatcaagcgc ggtgcggcga ccgttcggtt ggcctgatcg tcaggcggtt cgccgaacgc
     5581 tcgacatcgc ctggatcgcg tcgcccatac gatcgatcac gatctgcagg tcgttgccgt
     5641 agccctcgtc cacccgcacc gagagccggt cgatcttgtc cccgagcacc tcggccacct
     5701 tgaccatcga ctctgtggtc gactggctct ccttgtgcag ctggtcgttg atcgactcga
     5761 accgacggtc gaccgcggtc cggacatcgt tggccaggcc cgcctggagc tccttgatcg
     5821 cgagcatcgt ctggcggagc agctgcggat cgacggtctc ggcgggcgcg gcggtgctcg
     5881 ccttgaagga ctcggccagg atctggttgt ccgaccggat cagccgcgcg atcgccgtca
     5941 tgcggtcgtc gatgtgctcg cggagccgct cctcggtggc ggcctgtcct tcggccatca
     6001 tcgagtcgag tccgtcgatc gccccgacgc cggcgctcat gcgctcgatc gtctggccga
     6061 gcgagtcggc gatcgcccgc tcggcggcct cggacgcacg cgtcgcgacc tggtcaccgg
     6121 cccgttccag ctgttcgtcg atccgttcgg cgaacgccgc catacgccag tcgacggcct
     6181 cacggagggc ttcgacctgg tcctcggacc gttcctcgac cacgcggcgg atcgcctcgg
     6241 agtcagaacg gatcagctgg gccagaccca tgatgcgggc ctcgaccagc cgctcgatcg
     6301 ccgcgcgcac ctgatcttga tcgcgcccgt gcagcccgac cttctcggcc agcaggtcga
     6361 gttgctcggc gacgggccgg atccggaccg ccaccatgtc gcccacgtgc tcggagatcg
     6421 tcaggtcctg cgtcgcgaag gcgtcggcct gctcctcgat gcgggtcgcc aggatcgcca
     6481 tggcttcggc gccggtctgg acgtactgct gcatcacctc gacgaggcgc gtggtctcgc
     6541 gagccaccag ttcgccgtgc gccttgatct gcccgagcac gccctcggtg agcgcacggt
     6601 cgcgttcgtc gaagtgctcg aaagcggcgg cgatgtggtg ctcgacctgc tcgagctggc
     6661 tgcggacgcc ctccaccccg tgcagggtgg gagagtcggc gatctcgcgg atggccgcca
     6721 ccgaggcttc catcgaggcg cgggtggcgc ggccgctctc cgtgaggtcg gtcaacgaat
     6781 cctcgagccg cgccgagcgc agcgcgagcg actggaaccg ttcgtcgctg gagttgagga
     6841 ggctcttgat ggtctggtcg cgggacagca gcgagacgat gcgctcctgc tccgggcggg
     6901 cgtcggcacc cgcggatcgc cagatctcgc cggcgatctc gcgcatcagg gcggtcgctt
     6961 gttcctcgat ggccaggagg ccgtcggcga ggcgttcgtc cacggtgcgt tcgaccgccg
     7021 cggcgatgcg catctcaccg ggggtcatgc gtggttcgtc ttcgtactgc tcgagtcgac
     7081 gcaacagtcg ccgggatgcc ccggcgtgct gcggggaagc ggtggggccg tactcatcca
     7141 tgacgtcgct cgcgatctgg ggccgccgcg gggtgcgtcg cgaggagcat agggcacgac
     7201 ctggggcgac tcacgtatct gcaccaggga aaacgagggg tcctgtgagg tggttcagcc
     7261 gccggtgacg ggctcctcac cggggagcgt gaacccgtca tcaggcccat cgtcggtgag
     7321 cacggtctcg tctgccggga agcgttcgac gtgctgccgg cgccaggctc tggaaccggc
     7381 agggtcgttc aacaaggtgt cgacgtcggc ggcccacacc cggacgttga cgtcccaggg
     7441 gaagcgctcg acgccctcgg cgatgatccg cttcgcctcg gcgccggcct gttcatcacc
     7501 ggctcgaccc tgtgcggcga gttggagcgc cagttgctcg gtggacgtga gtcgccacgg
     7561 ctgcacgcgg agggcggctc gatacgccca ttcctctccg tagtggcgcc cccagcgctc
     7621 gaacgtggcg ccggcgatca tcgtgagcga caccaccagc gacaggctca agacgatccc
     7681 gaccgcgagg cgcacacctc gcggggttcg ctcatcagcg gcgggctccc ggggtcggag
     7741 cccggccgcc atggcggcga acaggaacag caggggcgtc aggaccaggt tgagcggttc
     7801 gaccaaggcg taggcgccca gggcggcggc ggcgcccagg ctccaggcgc gctccggacc
     7861 cgctcgccac gcccgcccca ccagcagcag gagcaacgcc acgagcggga tcaagccgag
     7921 gacccccgaa gtcacggtcg tttcgaggaa caggttgtga gcgtcggccc agcctcgggt
     7981 cgcctctgcg atgtcggcgg gcgtggcgct ggtcaggtac gccacctgcg tggtgccggg
     8041 cccccatccg acgagcggat gctcgccgat gctgccggac gtgacccgcc acatcacggc
     8101 tcggtcgaca tcggtggctt ccccttgcaa gggggcgagc gcggcgcccg ctcccgtctc
     8161 ccgcccggga agaacaggtt ctgcgatctg ccatccaagg aggaccacgc cgaccgtcgc
     8221 cgcgagggcg aagctggccc gccacggcat gcgggttcgt gccagcgtca cgatcacggc
     8281 gacggcgggc aggatcaagg agcttcgctc accgaaggaa gcggccccaa gcgccaggaa
     8341 ggccaccacc agcacctggt tccgcaggcg cttgccgggg accagcgccg cggcgatccc
     8401 cgccgcgagc agggccgccg cgaacagctg attgcccatc gtggccccca cgaacgagag
     8461 ggtcctcaag ccgagggtgt cggtcagcac gtcgggcgcg aaccggaaca cgagaccgag
     8521 tgctccgacg atgcacgagg cgaccacgaa ccagcgccgg cttcgttcgc gcaggtcggc
     8581 gtccacaccg acccccgtga ccacgatgac ggcacaactc agcaccagca tcaggcctcc
     8641 cccttcgccg tccgtacggg cggcgaggga gcggatgggc tcgaccccgg tgagcgcgct
     8701 caacgcggtg aagaccgacc acagcgccgc cagcgcgccg atccgccggt ccagggggag
     8761 tcccgaaccc cacagcgcca gcgatccccc gaccaggatg accagcttgg ggtcgccgaa
     8821 cgggctggtc gagaaggggg tgaaggcgag cggcagggcc acgaccagca ggaacaggcc
     8881 gagttcagcg agctgggggc ggcgggccgg catgacccga gggaatctag tccgggtccg
     8941 gttggaggtg gtggacgtga ctcggttccg acagaacatg tccggcgatc gcgagggcat
     9001 cctttgccgg gcttcgacgg agcgccctat actggtcgtt ccgcaagact ccccttcatg
     9061 ccgtggtggg gtgatacgga tcggcggagg cgcgtagctc aatttggtag agcaccggtc
     9121 tccaaaaccg aaggttgggg gttcaagtcc ctccgcgcct gcaaggagca ccatgaaccg
     9181 acagatgaag cggatgcagg agcgcgccga gcgccagcag aagcgcgcgg gcgtggaccg
     9241 ccaagctgcc ccggccgcga ccacgcgtcg cgcccagacg caggagaagc gtaagcgcac
     9301 cggcatccgt cagttcctga aggaggtccg tcaggaactt cggaaggtcg actggcccaa
     9361 ccgccgtgag ctggtctcct acaccgtggt cgtcctggcc accatcgtgg tcatgaccgc
     9421 cttcgtcgcc acgctcgact acgtcttctt gaagggcgtc gtcaagatct tcggctgacc
     9481 ccgcgaacca ccaacccgaa ggaacccatg gaagacctcg aacagagcaa cgacacgccc
     9541 gccgaggacg ccctcgaggc ggccgaggaa ctgagcgaac tcgccgacga cgctgcggcc
     9601 gaggccctga ccgaaggggt cgtcgaggcc gccgaggtcg aggcggtcgc cgatgccacc
     9661 gctgccgccg tgttggacga agccgccgac gaagccgagg cattgtccga agccggcgac
     9721 gacgaggcgg ccgaggaagt gctcgagcaa gggatcgtcg acgccgtcga gatcgagacc
     9781 gtcggtgagg ccgtcgccga cgaggtgctg gccgaggccg tcgaagaagc cgctgagatc
     9841 gaggaagccg ccgacgacgt gatcgaggaa ctcgtcgtgg aagccgtcgt cgccgaggcc
     9901 gtcgaagagg aggccgagct cgagatcggc gagaccgagt acctcgagat ggaggctgcc
     9961 caaggcgacg gcgaactccc gatcgccacg gagtccgtcg cggaagctgc ggtcgagccc
    10021 gtcgtcgaac ctgagcccgc cgccgagccg aaggctgccg agccggaacc cgaaccagag
    10081 ccgaagtccg acgacccgtt caagggcccc ggcgattggt acgtcgtgca cacctacgcc
    10141 gggtacgaga acaaggtcaa gaccaacctc gagtcgcgca tccacacgat gcagatggag
    10201 ggcaagatct tcaacgtcca catccccatg gaagacgtca tggagatcaa gggcggcaag
    10261 aagcaggtcg tccagaagaa ggtgttcccc ggctacctgc tcgtgaagat ggtgtacgac
    10321 aacgactcct ggtacgtggt gcgcaacacc cccggcgtga ccgggttcgt gtcggcaggc
    10381 accggcacca agcccacgcc gctcagccgc cgcgaggtcg agaagatcct cacggtcaag
    10441 aaggaagagg ccaagccgca ggttcgcctc ggcttcgacg agggcgacgt ggtgcggatc
    10501 atctccgggc cgttcgccga cttcaacggt acgatcagcg agatcaacgc cgaccaggcc
    10561 aagctcaagg tgctggtcaa catcttcgac cgcgaaacgc cggtcgaact gtccttcgag
    10621 caggtctcga aggtctaact cagacgcggg aggcgcttcg cgccgctggg accgcaggag
    10681 gtgggataca catggcaccc aagaaggtgc tcgcgagcgt caagctccag atcaaggccg
    10741 gtcaggccac gccagctccg cccgtgggga cagcgctcgg ccagcacggc gtgaacatca
    10801 tggagttctg caagcagtac aacgaggcca cgcagcagtt cagcggacag gtcatcccgg
    10861 tcgagctcac gatctacgag gaccggtcgt tctcgttcat cacgaagcag ccgcccgccg
    10921 ccgagctgat caaacaggcc gccggggtcg agaagggttc ggccgagccg aaccgcgaca
    10981 aggtggccag gatcagtccg tcccaggttc gtgagatcgc cgagcggaag atggccgacc
    11041 tcaacgccaa cgacctcgac aacgcgatgc gcatcatcga gggcacggcg cgcagcatgg
    11101 gcgtggaggt ggacgcatga gtcacggcaa gcgctaccgc gaggccgccg ggaacgttga
    11161 ccacgagcac gagtaccagc ccgtggaggc catcaagctc ctgaaggccc agcccgacgc
    11221 caagttcgac gagaccatcg agatcgcgtt ccgtctcggc ctggacacgc gcaaggccga
    11281 tcagtcgatc cgcggcactg tgtcgttgcc caacggcacc ggcaagtccg tgcgcgtcgg
    11341 cgtgttcgcc accggcgaga aggcgcgaga ggcctacgag gccggcgcgg acgtggtcgg
    11401 cggcgaggaa ctggtcgagc aggtcatgaa gggcgagatc gacttcgacg ccgccgtggc
    11461 cacgcccgac atgatggcct cggtcggtaa ggccggtcga gtcctcggtc cgcggggact
    11521 catgccgaac ccgaagaccg gcacggtgac gatggacatc gccaaggccg tcggcgacat
    11581 caagggcggc aaagtggagt accgcgccga tcgcaccggc aacgtccaca tggtgatcgg
    11641 caagaagtcc ttcggcgagc agcagctcct ggagaactac ctggccgtgg tcgacgaact
    11701 gctccgcgcc aagccgtcgt cggcgaaggg tcgctacatc gaatcgctcg ccgtgagctc
    11761 caccatgggg ccgagcgtcc ggatcgatcc cgcgcacccg aaggagatcg agatggcggc
    11821 cacctcggtc tagtcacccc gacatatccc atacggaacg ccccggcatc ggccggggcg
    11881 ttcgtgcgtt ccgacctgcc gaggaacctc cacatctcag cacgaaagtc cgggcaggaa
    11941 ggcgccgaag gtcatacgga tggcctaccc gcgcgttctg catgtcatag gaagccttga
    12001 acctggcggc acagaacgcc agttggtggg cttcatccag cgaagcggcg agccgggccg
    12061 gcatcacgtg gtcgtttgta gcccgggagg ggagctggag aaggagctcc ctcggtcccc
    12121 ggaccagatc gggccactca aacgtgaatt cctgggcgtt ccttcgggca tcagaaccgt
    12181 cgctgaattg catcgattga tctcccgtat cgagcctgac atcgtgcaca cccatctcgg
    12241 atatccgcag gtacttgcct ccatcgcggt accgcgcggc gtgcccatca tcgcttcccg
    12301 aagaggccgt accccgtacc tcgaatcctc cgggttcggt cggatgctgg tcggggtcgc
    12361 caatcgtcgc acgcgcctct tgttgaccaa ctcgcacgac cttgctcggc gggccgcgag
    12421 cgagcgttgg actccgccat cgagggtgat ccccaacggg gttgacttgg atcggttcac
    12481 cccggcgccg ttccccgaga ctgggtcccc gaccgtcgtg atgctcgcgc gcatgcgtgc
    12541 cgagaaacga cacgatcgtt tcctgcgggt gttccgccga gttgtcgacc aactaccaga
    12601 tgcccgagcc atcctcgtgg gggacggacc gtgtcgggac gatgtggctc ggctggttgg
    12661 cctcatgggc ctctctgaaa gggttgatct cgtgggcggg tgcgacgatc cccgaagtca
    12721 actggcgcgg tcccacgtcg tgacgttgac gtcggatcac gaagggtttc caaacgctct
    12781 cttggagggc atggcgatgg gccggcccat cgtggcgacg gacgtaggtg gtgtcgcaga
    12841 actggttggc gaccaagcgg ggatcaccgt ccc
//
LOCUS       DATDBZ010000205         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1069503, whole genome shotgun sequence.
ACCESSION   DATDBZ010000205 DATDBZ010000000
VERSION     DATDBZ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069503"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1275)
                     /locus_tag="VIX62_05165"
     CDS             complement(121..1275)
                     /locus_tag="VIX62_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5859653.1"
                     /translation="MSEPIGIGVVGAGMIAQRGILPHLSQDDVRDRLRVVAVCDPAPG
                     RAAASAERFGITTPYDAFEELLADPAVDAVSIASPIGLHFAQARAALEAGKHVHVNKT
                     MSTTTAEADELIALAVDRSLSLVASPGEVLRPQLTAIRTLIARGAIGQLCWGICGCSL
                     GNYHEADEEAERTSVPGGVPADPSWYFRQPGGGPMYDVTVYALHQLTSVLGPARRVTA
                     MSGTVVPSRTFLGTEIVTEVDDNTIQLLDFGDGVQVVVYGTAAGNVIEDFAAGQYFGT
                     EGSIVGLQLNGAPIEFEGRELTTDRPTWDWDAQMRVLPHVVGVHREIPEAHVFEDIMQ
                     LVGLVRDGTPTPVTPEHSRHVIEIIEAGYRAAETGQTQPLRTTFEFVAGP"
     gene            complement(1300..>2086)
                     /locus_tag="VIX62_05170"
     CDS             complement(1300..>2086)
                     /locus_tag="VIX62_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5859654.1"
                     /translation="PLGTSVDGATRLRDEAAARGLVLVAAPSVLRFPQVLEARRLLAE
                     GVIGAVHTVRGLGFGGVPPWEGYGSDPGPFFAPGAGPLVDLGVYPLHAITGLVGPAMR
                     VSAMSARTRDAFDIADGPLAGRRIPVGVDDAWVVTLDLGEARLATVEANFTARGSRSA
                     ELELMGETGTIALSLLDVAAPVQVLDTRGAWTTLEVETGRPAGGPDHILGVEHLVDCI
                     RVGTTNMLPAQHAIHVIEILDAAGRSAADGSSVDLRTTFDLMN"
BASE COUNT          343 a          742 c          685 g          316 t
ORIGIN      
        1 gccccctcgt gcgccggtgc tcggccgcaa gcgagcgccc accttcggca cgccatggga
       61 gttccctgag ggcttcgacg gctgggcacc gggcacggtc cccaacgaag accacttcgt
      121 ctaagggcct gccacgaact cgaaggtcgt tcgcaggggc tgcgtctggc cggtctcggc
      181 ggcgcggtaa ccggcttcga tgatctcgat gacgtgacgt gaatgttcag gcgtgaccgg
      241 cgtgggcgtg ccgtctcgaa ccagcccgac caattgcatg atgtcctcga acacgtgcgc
      301 ttcagggatc tcccggtgca cgccgacgac gtgcgggagc acgcgcatct gggcgtccca
      361 atcccaggtc ggacggtccg tcgtgagctc gcgcccctcg aactcgatcg gtgcaccgtt
      421 gagctggagc ccgacgatcg agccttcggt cccgaagtac tgtcctgccg cgaaatcctc
      481 gatgacgttg cccgcggcgg tcccgtacac caccacctga accccatccc cgaagtcgag
      541 caactggatc gtgttgtcgt cgacttcggt gacgatctcg gtgcccaaga aggtccggct
      601 cggcacgacc gtgcccgaca tcgcggtgac ccgtcgggca gggcccagca cgctcgtcag
      661 ctggtggagc gcgtagacgg tcacgtcgta cataggcccg ccgccgggct ggcggaaata
      721 ccagctggga tcggccggca caccgcctgg caccgacgtc cgttcggctt cctcgtccgc
      781 ctcgtggtag ttaccgagtg agcagccgca gatcccccag cacagctgcc cgatcgcccc
      841 tcgtgcgatc aaggtgcgga tcgccgtgag ttgtggacga agcacctcgc ccggagaggc
      901 cacgagcgac aacgaccgat cgaccgccag cgcgatcagc tcgtcggcct cggcggtcgt
      961 cgtcgacatc gtcttattga cgtgcacgtg cttgccggcc tcgagcgcag ctctggcctg
     1021 tgcgaagtgc aacccgatcg gtgaggcgat cgagacagcg tcgacggctg ggtcagccag
     1081 gagttcctcg aacgcgtcgt agggcgtcgt gatgccgaag cgctccgccg aagcggcagc
     1141 tcggccggga gccggatcac acacggcgac cacgcgcaac cgatcccgca catcgtcctg
     1201 gctgagatgc ggcaagatcc cgcgctgcgc gatcatgccg gcgccgacga cgccgatacc
     1261 gatgggctcg ctcacgtgct cctccttgcg gtcggacggt cagttcatga ggtcgaacgt
     1321 ggttcgtagg tccacggagg aaccgtcggc ggccgaccga cctgctgcat ccaggatctc
     1381 gatgacgtgg atcgcatgct gggcgggcag catgttggtc gtgccaactc ggatgcagtc
     1441 cacgagatgc tcgacaccga ggatatgatc gggtccgccg gccggacgcc cggtttcgac
     1501 ctcgagcgtg gtccaggcgc ctctggtgtc gagcacctgg acaggtgcgg cgacatcgag
     1561 caggctcaac gcgatcgtgc cggtttcgcc catcagctcg agctcggccg atcgagagcc
     1621 ccgagccgtg aagttggcct ccaccgtcgc gagccgtgcc tcgcccagat cgagggtcac
     1681 cacccacgcg tcgtcgaccc ccaccgggat ccgccggccc gccaacgggc cgtcggcgat
     1741 gtcgaaggcg tctcgtgtgc gagcactcat cgcggacacc ctcatcgccg gacccaccag
     1801 accggtgatc gcgtgcagcg ggtagacacc cagatcgacg agcggacccg cacccggcgc
     1861 gaagaacggc ccgggatccg acccgtaccc ctcccaggga ggcaccccgc cgaacccgag
     1921 gccacgcacg gtgtgcaccg cgccgatgac gccctcggcg aggagccttc gcgcctccag
     1981 cacctggggg aagcgcagca cgctgggcgc ggcgaccaac acgaggccgc gggcagccgc
     2041 ctcgtctcgg agccgcgtcg ccccgtccac cgacgtcccc agcggc
//
LOCUS       DATDBZ010000206         2262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2524530, whole genome shotgun sequence.
ACCESSION   DATDBZ010000206 DATDBZ010000000
VERSION     DATDBZ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524530"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VIX62_05175"
     CDS             complement(<1..551)
                     /locus_tag="VIX62_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY5859655.1"
                     /translation="MKIGVIRGDGTGPEVVAEGLKVLDAVKGDAAFELVEFDLGAERY
                     LRTGDVLPDEELDGLREMDAVFLGAVGDPRVKPGILERDLLLKARFGLDQYVNLRPVV
                     RYPGVETLVPSHSEDDVDFVVVRENSEGLYTGAGGFHRKGTQYEVAVQESINTRHGVE
                     RIVRYAFELAMRPDRRRKLTLVH"
     gene            complement(548..2110)
                     /locus_tag="VIX62_05180"
     CDS             complement(548..2110)
                     /locus_tag="VIX62_05180"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY5859656.1"
                     /translation="MSDGAGNVGNVRIFDTTLRDGEQAPGIALTKAEKVEIAEQLARL
                     NVDILEAGFPISSDGEFDAVREIADTVKGPVIAALARTATGDIERAAEALKGADRWRI
                     HTFISTSDVQRESMLKMSYDQVLNAIRENVKLAKSYTEDVEFSAQDATRTPLEFLLEC
                     FKLAVQCGATTINVPDTVGYATPTEFGELIRVVRDQTPDDVTISTHCHNDLGLAVANS
                     LAGIENGAGQVEVAVNGIGERAGNCSLEEIAMVVRTRGQALERSHGLNIREIVRTSRL
                     VAMTTGYSVQPNKAVVGSSAFAHESGIHQHGVLSNRATYEIMDPVEIGLEGNSLVLGK
                     HSGRHAFIDALDKIGVTLDEDHMNRAFGRFKDLADRKVQITDQDLSAIVAEELGATDE
                     DVLVMESLQMGGGTHLQPRATVRLTKGEEVVEESAMGDGLIDAVMGAIQRASGVEGRL
                     VNFHVSSVTGGTDALGDVVIQIDVDGRRFTGRGVATDVVEASARAYLAAVNRALQTGA
                     PREVTERTQVTP"
BASE COUNT          321 a          806 c          728 g          407 t
ORIGIN      
        1 ttgtgcacca gggtgagctt ccggcggcgg tcgggccgca tcgcgagctc gaacgcgtag
       61 cgcacgatgc gctccacgcc atggcgcgtg ttgatcgact cctgcaccgc cacctcgtac
      121 tgcgtgccct tgcggtggaa cccgcccgcg cccgtgtaga gcccctcgga gttctcgcgc
      181 acgacgacga agtcgacgtc gtcctcgctg tggctgggca cgagggtctc caccccgggg
      241 taccgcacga ccggccgcag gttcacgtac tgatccagcc cgaaccgggc cttcagcagg
      301 aggtctcgct cgaggatgcc tggcttcacg cgtgggtcac ccaccgcacc caggaagacg
      361 gcgtccatct cgcggaggcc atcgagctcc tcgtcgggca ggacgtcgcc ggtgcgcagg
      421 taccgctcgg ctcccaggtc gaactcgacc agttcgaacg ccgcgtcgcc cttgaccgca
      481 tcgagcacct tcaggccctc ggcgaccacc tcgggtccgg tgccgtcccc ccggatgacg
      541 ccgatcttca cggcgtcacc tgggtgcgtt ctgtgacctc gcgaggcgca cccgtctgca
      601 gggcccggtt caccgcggcg aggtaggcgc gagcgctggc ctccacgacg tcggtggcga
      661 ccccgcgacc ggtgaagcgg cgtccgtcca cgtcgatctg gatgaccaca tcacctaagg
      721 catcggtgcc gcccgtgacg ctggacacgt ggaagttcac gagtcgacct tcgacgcccg
      781 acgcccgttg gatcgcgccc atcacggcgt cgatcaggcc gtcgcccatc gcggattcct
      841 ccaccacctc ctcgcccttg gtcagacgaa cggtggcacg cggctgcagg tgcgtgccgc
      901 cgcccatctg cagcgactcc atcaccagga cgtcctcgtc ggtggcgccg agttcctcgg
      961 ccacgatcgc cgacagatcc tggtcggtga tctgcacctt gcggtcggcc aggtccttga
     1021 agcgaccgaa cgcccggttc atgtggtcct cgtcaagggt gacgccgatc ttgtcgagcg
     1081 cgtcgatgaa cgcgtgccgg ccggaatgct tgcccaacac cagcgagttg ccctcgaggc
     1141 cgatctcgac cggatccatg atctcgtagg tggcgcggtt gctgagcacg ccgtgctggt
     1201 ggatgccgct ctcatgcgcg aacgcgctgg agcccaccac cgccttgttc ggctgcacgc
     1261 tgtacccggt ggtcatggcg accagccgag acgtccgcac gatctcgcgg atgttcagcc
     1321 cgtgactgcg ctcgagcgcc tgtcctcggg tacgtaccac catggcgatc tcttccagcg
     1381 aacagttgcc cgctcgctcg ccgatgccgt tcacggccac ctcgacctgt ccggcgccgt
     1441 tctcgatgcc ggccagggag ttggcgaccg ccaggcccag gtcgttgtgg cagtgcgtcg
     1501 agatcgtgac gtcatcgggc gtctggtcgc gcacgacccg gatcagctca ccgaactcgg
     1561 tgggcgtggc gtagcccacg gtgtcgggca cgttgatcgt ggtggcgccg cactgcacgg
     1621 cgagcttgaa gcactccagc aggaactcca gcggggtgcg cgtggcgtcc tgcgcgctga
     1681 actccacgtc ctcggtgtag ctcttggcga gcttcacgtt ctcgcggatc gcgttcagta
     1741 cctggtcgta gctcatcttc agcatcgact cgcgctgcac gtcgctcgtg gagatgaacg
     1801 tgtggatccg ccagcggtcg gcgcccttca gcgcttcggc ggcgcgctcg atgtcgccgg
     1861 tggcggtgcg cgcgagcgcc gcgatcaccg gacccttcac cgtgtcggcg atctcgcgca
     1921 cggcgtcgaa ctccccgtcg gaggagatcg ggaacccggc ttcgaggatg tcgacgttca
     1981 gccgggcgag ctgctcggcg atctcgacct tctcggcttt cgtgagcgcg atcccgggag
     2041 cctgctcccc atcgcgcagc gtggtgtcga agatcctgac gttgcctacg ttgcctgctc
     2101 catcgctcat accggcggca ccttcttctc gctctccaac cagggcatca tctggcgcag
     2161 ctgccgtccc acgtcctcga gctgggacga gcgcgcctgg ctcctaagtc ggaggaactc
     2221 cgggaacccg gcgtcggcct cggcgatcca gttcctggcg aa
//
LOCUS       DATDBZ010000207         3051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_276250, whole genome shotgun sequence.
ACCESSION   DATDBZ010000207 DATDBZ010000000
VERSION     DATDBZ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276250"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VIX62_05185"
     CDS             complement(<1..374)
                     /locus_tag="VIX62_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859657.1"
                     /translation="MRQFFPVEDLARDERFVQTNMAERMKDARQPRIKDAPASGTRSS
                     AKSRAATARLTPDRPDASDAARSLMHGIMVGEIQALEGAGRTAHDFASGDGTNDTIPF
                     ELKLDMARQAWDEARHVEISVKL"
     gene            complement(415..1053)
                     /locus_tag="VIX62_05190"
     CDS             complement(415..1053)
                     /locus_tag="VIX62_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859658.1"
                     /translation="MASTTDSPSTARAPFAPWDRRDLPGSFDVVETARRVGHYKWTEM
                     KLFEALGGWIATIPELDVKMRLGTHCYHHAWHADLWHKRLPELREMKPERLTVPANDA
                     MERFIEAMTEPEAPELTIEKLVGVYRVLIPRFIAAYTYHQNNTSEITDAPTIRTIKFI
                     LQDEFDDWRDGELMLQSLITTSDHVRRAADHQARLEAIMVEAGGITGPGSIG"
     gene            complement(1226..2692)
                     /locus_tag="VIX62_05195"
     CDS             complement(1226..2692)
                     /locus_tag="VIX62_05195"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEY5859659.1"
                     /translation="MQRRAGPPVASLVTVASLVALAAGLAAAAQRARSSPLTSRFARS
                     AKIWRLSARNSTRFAVSKVRGFRSPEERRAELDEQFAIRTAEDVAKELGEMKGVLMKA
                     GQLISFIFEALPDEAQAALATLQADAEPMAPTLAADVVKADLGRAPERVFLEWGDMPV
                     AAASIGQVHRAITPDGRDVAVKIQYPGVREAIESDLDAAEVMYAMFSAMMLKGLDAKG
                     LVDELRARMREELDYRLEARNIAEFEERFRGHPWVRIPKLVPEFSTEHLLTTEWIDGM
                     SFDEFRTNESYATKQRAGEVIWRFAQNAIFRHGVFNGDPHPGNYKFHHDGSVSFLDYG
                     LVKRWTRGEWDGLRPTMDAIIIDRDPDLLVTRMEASGFLRSGHGLDPALVYDYVSSPY
                     TPYLSDEFTFTREWMRDTIGVIFDVQGPHGPVIEQLNMPPSFVILDRVVWGVSAILGK
                     LEAKAPWRAMLLEYTGGGPPATDLGAAEAAWFATRPVH"
     gene            complement(2692..2907)
                     /locus_tag="VIX62_05200"
     CDS             complement(2692..2907)
                     /locus_tag="VIX62_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859660.1"
                     /translation="MSDHAPPVPPLGPQPADVVRADAERVDLDRIERELRDVETALAR
                     LADGTYWSEPGALVPGTRAPGLDAPDH"
BASE COUNT          432 a         1011 c         1073 g          535 t
ORIGIN      
        1 agcttgacgc tgatctccac gtgccgcgcc tcgtcccacg cctggcgggc catgtcgagc
       61 ttgagctcga acgggatcgt gtcgttggtg ccgtcgccgc tggcgaagtc gtgcgccgtg
      121 cgtccggcac cctcgagcgc ctggatctcg ccgaccatga tcccgtgcat cagcgagcgg
      181 gcggcgtcgg atgcgtcggg ccggtcgggc gtgagccgcg ccgtcgcggc gcggctcttg
      241 gccgacgatc gcgtgccgct cgccggagcg tccttgatgc gcggctggcg cgcgtccttc
      301 atccgctcgg ccatgttcgt ctgcacgaag cgttcgtctc gggcgagatc ctcgactggg
      361 aagaactggc gcatcaggta cccccgtact cgtgctcgtg gtcgtgtccg atcgtcatcc
      421 gatgctaccc gggcccgtga tcccgcctgc ctccaccatg atcgcctcga gcctggcctg
      481 atggtcggcg gcgcggcgga cgtggtcgga cgtggtgatg agcgactgca gcatcagctc
      541 gccgtcgcgc cagtcgtcga actcgtcctg caggatgaac ttgatggtgc ggatcgtcgg
      601 ggcgtcggtg atctcgctcg tgttgttctg gtggtacgtg taggccgcga tgaaccgcgg
      661 gatcagcacc cggtagacac cgacgagttt ctcgatcgtg agttccggcg cctccggctc
      721 ggtcatcgct tcgatgaagc gctccatcgc gtcgttggcc gggacggtga gccgttccgg
      781 tttcatctcg cggagttcgg ggaggcgctt gtgccacagg tcggcgtgcc acgcgtggtg
      841 gtagcagtgc gtgccgaggc gcatcttcac gtcgagttcg gggatcgtgg cgatccaccc
      901 cccgagcgcc tcgaacagtt tcatctcggt ccacttgtag tgaccgaccc ggcgcgcggt
      961 ttcgacgacg tcgaacgacc cgggcaggtc gcgccggtcc cacggtgcga acggtgcacg
     1021 tgccgtggag ggactgtcgg tcgtggaggc catcgccgcg accgtagtag ccgtccctcc
     1081 ccgcggattg accggacgtg tcaatccgcg aacggcggaa cccgggtgcg cccacaagtc
     1141 ccgtttgcgt cacgatttct ggggaccggt gcccagaaac tgtgacgcaa acgatggtgg
     1201 gggacgggac ggctggtggg tcgggtcagt gcacggggcg ggtggcgaac caggcggctt
     1261 cggcggcgcc gagatcggtg gccggcggac cgccgccggt gtattccagc aacatggctc
     1321 gccacggggc cttcgcctcg agcttgccga ggatcgcgct gaccccccac accacccggt
     1381 cgaggatcac gaagctcggc ggcatgttca gttgctcgat caccggaccg tggggtccct
     1441 gcacgtcgaa gatcacgccg atcgtgtcgc gcatccattc gcgggtgaac gtgaattcat
     1501 cggacaggta tggcgtgtac gggctcgaca cgtagtcgta gacgagtgcc ggatcgaggc
     1561 cgtggcccga ccgcaggaaa cccgacgcct ccatgcgcgt gacgaggagg tcgggatcgc
     1621 ggtcgatgat gatcgcgtcc atcgtcggac gcaggccgtc ccattcgccc cgggtccagc
     1681 gcttcacgag gccgtagtcg aggaacgaga cgctgccgtc gtggtggaac ttgtagttgc
     1741 ccgggtgcgg atcgccgttg aacactccgt gccggaagat cgcgttctgg gcgaaacgcc
     1801 agatgacctc gccggcgcgc tgcttggtgg cgtacgactc gttcgtgcgg aactcgtcga
     1861 agctcatccc gtcgatccac tcggtcgtga gcagatgctc ggtgctgaac tcgggcacca
     1921 gcttggggat gcgcacccac ggatggccgc ggaagcgctc ctcgaactcg gcgatgttgc
     1981 gggcctcgag gcggtagtcg agttcctcgc gcatccgggc gcgcaactcg tcgacgagac
     2041 ccttggcgtc gaggcccttc agcatcatcg ccgagaacat ggcgtacatg acctcggcgg
     2101 cgtcgagatc gctctcgatc gcctcccgga caccggggta ctggatcttc acggcgacgt
     2161 cgcggccgtc cggtgtgatc gcccggtgga cctgcccgat cgacgcagcg gcgaccggca
     2221 tgtcgcccca ctcgaggaac acgcgctcgg gtgcccgtcc gagatcggcc ttcaccacgt
     2281 cggcggcgag cgtcggggcc atcggctcgg cgtcggcctg cagtgtggcc agcgcggcct
     2341 gggcctcgtc gggcagggcc tcgaagatga agctgatgag ctgtcccgcc ttcatcagca
     2401 cccccttcat ctcgcccagc tccttggcga cgtcctcggc ggtgcggatc gcgaactgct
     2461 cgtcgagctc ggcgcgccgt tcctcggggc tgcggaaccc gcggaccttc gacacggcga
     2521 agcgggtgga gttgcgggcc gagagcctcc agatcttggc cgagcgggcg aaccggctcg
     2581 tcagcggcga cgacctggcc cgttgcgcag cggcggccag cccagcggcg agtgcgacca
     2641 gcgacgcgac cgtcaccagc gaggcgaccg ggggacccgc cctgcgctgc atcagtggtc
     2701 cggcgcgtcc agacctggtg cacgagtgcc tggcaccagt gcaccaggtt ccgaccagta
     2761 ggtgccgtcg gcgaggcggg cgagtgcggt ctccacgtcg cggagttcgc gttcgatccg
     2821 gtccagatcg acgcgttcgg cgtcggcacg cacgacgtca gctggctgcg gcccaagtgg
     2881 cggcacgggc ggcgcgtggt cgctcatccg ctgcacccta attcgttccc gccgcggcaa
     2941 cctcgccgct cactcggtga cgaatagggt gaggtcgatg ccagacaagc cgcgatccgt
     3001 acggcgttgg gccgacttcc cgctggcgtt ggcgatgctg ctgccgtccg c
//
LOCUS       DATDBZ010000208         6252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_44619,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000208 DATDBZ010000000
VERSION     DATDBZ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44619"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VIX62_05205"
     CDS             <1..719
                     /locus_tag="VIX62_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859661.1"
                     /translation="ARVAELTERWPATLVLTDLLSRDTWDLRPCDLSARRLLLVSIAE
                     DSDIPAASADLGQLATGTPVVVTPHTPFVSFAIRWLEDRDESGRDGVIARAMDGVTMV
                     RVPRLRTATCVVTGIRREGSGPPHALRLGLYDGDALVDVGRTTSLRGSRERRGAALAL
                     AGLERDTRAVNGWTDVRPEMACEVTFARLRGRRFRSAARFMRWLPAMDPQRCTLQQFD
                     AMAPPPPAPADDHDVIRLDV"
     gene            complement(729..1406)
                     /locus_tag="VIX62_05210"
     CDS             complement(729..1406)
                     /locus_tag="VIX62_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5859662.1"
                     /translation="MQVGIVGTGTVGRTLAQGFTQKGHDVVIGTRDVEALLARTEGDA
                     MGNPPFAQWKAEHPEVGLTTQADAMAHGEIVVNATDGEGSEAALSAGGEHLTGTIVLD
                     ASNPIDHASGWPPSLFVANTDSLGERLQSAFPQARFVKVWNTMTAALMTDPGALAGGD
                     HTIPLCGNDADAKAEVAAILESFGWRDILDLGDITAARAMEAHLLIWLRELGALDTAM
                     FNTKVVR"
     gene            1530..1940
                     /locus_tag="VIX62_05215"
     CDS             1530..1940
                     /locus_tag="VIX62_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011455423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859663.1"
                     /translation="MTAKGSGTPEDPWVLQTPSLQSEYLMARAPDADPPALICTVGKT
                     ELRYHLRAIEDLHAMLVAHGDWMPLGNADEQKPAKDGTVEAWARSTDNPVGGWYGLKK
                     GLRGRFGNYVPPVLEVLGLAEVEHNPRNNRMRAI"
     gene            2062..2364
                     /locus_tag="VIX62_05220"
     CDS             2062..2364
                     /locus_tag="VIX62_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007153503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY5859664.1"
                     /translation="MTTVGLLVRLKAKEGKEREVQRFLESGRTLVEAEPATTAWFAIK
                     LGPSEFGIFDAFPDDAGRQAHLAGRVAEALMAQADDLLSEPPDIQPVDVLTEKLPG"
     gene            2420..2893
                     /locus_tag="VIX62_05225"
     CDS             2420..2893
                     /locus_tag="VIX62_05225"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY5859665.1"
                     /translation="MRWWVVLHVVIAFGFVAGLIGRDITLAKARSTDDLGQVGALVGL
                     ASRFEKLLVIPGSFAVLVAGLIAMVAQGRSLTGPRNGWLVVSLVLFVALFALVPTVFL
                     PRGKVFDVALEDATAKGTMTPELQRAFADPVVAFARNAELVAVVVIIALMVAKPF"
     gene            complement(2900..3385)
                     /gene="soxR"
                     /locus_tag="VIX62_05230"
     CDS             complement(2900..3385)
                     /gene="soxR"
                     /locus_tag="VIX62_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEY5859666.1"
                     /translation="MTKSREDLLTIGEVAERSGLATSAIRFYEAEGLVTSTRTPGNQR
                     AFARHTLRRLAFIRAAQRVGLSLDDVAGALATLPPDRAPTKAQWAKLSRTWRRGLDER
                     IADLEALRDDLTSCIGCGCLSLATCRLYNPDDAASVNGSGPRFLLGDDHDGFLETGRR
                     A"
     gene            3447..4136
                     /locus_tag="VIX62_05235"
     CDS             3447..4136
                     /locus_tag="VIX62_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5859667.1"
                     /translation="MDTTTDPLTHVRALMARATGDEKHDESTTSTLDALWVLYDRVLR
                     IDPAHPDAEDRDRFILSKGHGPASLYAILAAKGFFPPDWLDAFLDHDGRLGSHPDRMQ
                     VPGIEASTGSLGHGFPMAVGVAQALRAKGRPDTQVVVLTGDAELNEGSNWEAVMLAPN
                     LRLGNLTWLIVDNHSSSWTMGPWDAWLPPFGWDVRVVDGHDHDALAAALGDRHAEQPV
                     AVVADIPEGEW"
     gene            4136..5011
                     /locus_tag="VIX62_05240"
     CDS             4136..5011
                     /locus_tag="VIX62_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5859668.1"
                     /translation="MAAMREAFAAAATDLLEADERVAIVLADISETLLRRAIRHDPLR
                     AVNVGIAEQTMVGVAAGFAMEGFHPIAHSLSPFMAERPYEQLKLDFGYQGLGGTFVGS
                     GGSYDYAGEGATHHSPADASLMLAIPRMQVLVPGHGREVDQLLRAVVANGEPTYVRTS
                     LATNDEPHDVAPGRLEVIRRGAGPTVVAFGPMLTRALEATAHLDATVVYATSLEPFDG
                     PTLRELARDAPDIVVVEPWYEGTAAAVITAVFADVAVRLRSIGVPRRFIHRYGTWQEL
                     DASLGMDATGIRARL"
     gene            5069..>6252
                     /locus_tag="VIX62_05245"
     CDS             5069..>6252
                     /locus_tag="VIX62_05245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5859669.1"
                     /translation="MQEAQRSTDALVDPAQLFAAGPEALLVVREGRIACANERALDLV
                     GRPIAGLDLHEVIPDWRAAPDDAVPFEAMLRCADGELAIEVRVRELEDGVTVASLRDA
                     RALIAGREAEAALADAEERYRGLVEQIPAVVYADDGNVTTYVNPQIEHILGVTPQAYR
                     DDPNMWLRMVHPDDRETVESQSQAFIDGVGGDLADYRMIRPDGGIVWVRDRAYAHRDD
                     DGRVLWEHGILFDVTELKEAEARIAHMAFHDGLTGLANRELFEETLALAVQRAKRDGT
                     VVAVLYLDLDNFKLVNDSLGHHAGDRLLIQLADRLSACTRDTDLVARQGGDEFLMLLA
                     DLGHADYVEAIRAVTDRVNEAMAAPFDLEGTEFFARGSIGISVHPHDAHDVETLMRNA
                     DVA"
BASE COUNT          919 a         2135 c         2203 g          995 t
ORIGIN      
        1 aggcgcgggt cgccgaactc accgagcggt ggccggcaac gctcgtgctg accgacctgc
       61 tgtcgcgcga cacctgggat ctgcggccgt gcgacctctc ggcgcggcgc ctgctgctgg
      121 tctcgatcgc ggaggattcg gacatccccg cggcctcggc cgacctcggc cagctggcaa
      181 ccggcacgcc ggtcgtcgtg acgccgcaca cgcccttcgt cagtttcgcg atccggtggc
      241 tcgaggaccg ggacgaatcc gggcgcgacg gcgtgatcgc gcgagcgatg gacggggtca
      301 cgatggtgcg ggtgcccagg ctccgaacgg cgacctgcgt ggtgaccggg atccggcgcg
      361 aaggctccgg cccgccgcac gccttgcgcc tggggctcta cgacggcgat gccctggtcg
      421 acgtggggcg gaccacgtcg ctccgtgggt cgagggaacg gcgcggagcg gcgctcgcgc
      481 tcgcaggtct cgagcgtgac acccgggccg tgaacggatg gaccgacgtc cgccccgaga
      541 tggcctgcga ggtgacgttc gcacggctgc gcgggcggcg gttccgatcg gcggcgcggt
      601 tcatgcggtg gctgccggcg atggatccgc aacgatgcac gctgcagcag ttcgacgcga
      661 tggcaccgcc tccgccggcg cccgccgacg atcacgacgt gatccgcctc gacgtctgag
      721 gctcgccgtc agcgcaccac cttcgtgttg aacatcgcgg tgtccagcgc gcccaactcg
      781 cgaagccaga tcaggagatg agcctccatg gcgcgagccg cggtgatgtc gcccaggtcg
      841 aggatgtccc gccagccgaa ggattccagg atcgcggcga cctcggcctt ggcgtcggcg
      901 tcgttgccgc acagcgggat cgtgtggtcg ccgccggcga gggcgcccgg gtcggtcatc
      961 agcgccgccg tcatcgtgtt ccacaccttc acgaatcgcg cctgagggaa ggccgactgc
     1021 aaccgttcgc cgagcgaatc ggtgttggcg acgaacaagc tgggcggcca acccgacgcg
     1081 tggtcgatcg ggttggacgc gtcgagcacg atcgtcccgg tgaggtgttc gcccccggcg
     1141 ctcagcgcgg cctcggagcc ttcaccgtcg gtggcgttca cgacgatctc gccgtgggcc
     1201 atcgcgtcgg cctgggtcgt gagcccgacc tccgggtgct ccgccttcca ctgcgcgaac
     1261 ggtgggttcc ccatggcgtc gccctcggtg cgcgccaaca acgcctcgac gtcgcgcgtc
     1321 ccgatcacga cgtcatggcc tttctgggtg aatccctgcg ccagggtccg accgacggtt
     1381 cccgtgccca cgatgccgac ctgcatctgc cgctcccttc gtcgtggcac catcttggga
     1441 catgccccac gttccgaccc cgggcccctc gcgcacctag ggtggatcca ggggtgacct
     1501 tcccacgcgt ctcgcgctac ggttcaccca tgacggcgaa aggaagcggc acgccggaag
     1561 acccgtgggt gttgcagacg cccagcctcc agtcggagta cctgatggct cgggcgccgg
     1621 acgcggatcc gccggcgctg atctgcacgg tcggcaagac cgaacttcgc tatcacctgc
     1681 gggcgatcga ggacctgcac gcgatgttgg tggcccacgg cgactggatg ccgctgggca
     1741 acgccgacga gcagaagccc gcgaaggacg gcacggtcga agcatgggct cgctcgaccg
     1801 acaacccggt gggcgggtgg tacggattga agaaagggtt gcgcgggcgc ttcggcaact
     1861 acgttccgcc ggtgctcgag gtgctgggtc tcgccgaggt ggagcacaac ccccgcaaca
     1921 accggatgcg cgctatctga ctgcgcacag ttgtgtgcga cctgactgcg cacaatggtg
     1981 gtgtgcgacc tgacggcgcg ccgtcgtggg cgacctgaca taggctgatc gcagcccgtg
     2041 ttccccgaag gaaggacccc catgaccacc gtcggactcc tcgttcgcct caaagccaaa
     2101 gaaggcaagg agcgggaggt gcagcggttc ctcgagtcag ggcgcaccct cgtcgaggcc
     2161 gaacccgcca ccaccgcctg gttcgcgatc aagctggggc cgtcggagtt cgggatcttc
     2221 gacgccttcc cggacgatgc cggacgccag gcccacctcg ccggtcgcgt cgccgaggcg
     2281 ctcatggccc aggccgacga cctgctgtcc gagcctccgg acatccagcc ggtcgacgtg
     2341 ctcaccgaga agctccccgg ctgacccttc gccctacacc acgtagcctt caccctgtcc
     2401 gaaccgtcag gagggcgtga tgcggtggtg ggtggtgctg cacgtggtga tcgcattcgg
     2461 gttcgtcgcc ggcctcatcg gccgcgacat caccctcgcg aaggcgcgtt ccaccgatga
     2521 cctgggccag gtgggggcgc tcgtcggcct ggcgtcgcgg ttcgagaagc tgctggtgat
     2581 ccccgggtcg ttcgccgtgc tggtagcggg cttgatcgcg atggtggcgc agggccgctc
     2641 gctcaccggc ccacggaacg gctggctcgt ggtgtccctg gtgctgttcg tggcgctgtt
     2701 cgccttggtg ccgacggtgt tcctgccgcg agggaaggtg ttcgacgttg cgctcgagga
     2761 tgcgacggcg aagggcacga tgacgccgga gctccagcgg gcgttcgccg atccggtggt
     2821 ggcgttcgct cggaacgccg agctggtcgc ggtggtcgtg atcatcgcac tgatggtggc
     2881 caagccgttc tgacctgcgt cacgcccggc ggccggtttc gaggaatccg tcgtggtcgt
     2941 cgcccagcag gaaccgtggc ccgctgccgt tgacgctcgc ggcgtcgtcg gggttgtaga
     3001 ggcggcaggt ggcgagcgac aggcagccgc agccgatgca gctggtgagg tcatcccgca
     3061 gcgcctcgag gtcggcgata cgttcgtcca gcccgcgtcg ccacgtgcgg gagagcttgg
     3121 cccattgcgc cttcgtcggg gctcggtcgg gcggcagggt ggcgagcgcc ccggcgacat
     3181 cgtccagaga gagccccaca cgttgcgccg cacggatgaa cgcgaggcgc cgcagcgtgt
     3241 gtcgcgcgaa cgcccgctgg ttgcccggcg tcctcgtgga cgtgaccaat ccctcggctt
     3301 cgtagaagcg gatcgccgag gtggcgagac cgctccgttc cgcgacctcg ccgatcgtca
     3361 gcaggtcttc gcggctcttc gtcatggcgg tcacctcagc gcggggcttg acttcaagtg
     3421 aacttgaagt tctagggttc gtcgtcatgg ataccacgac cgatcccctg acgcacgtgc
     3481 gtgcgctgat ggcgcgggcc accggcgacg agaagcacga cgagagcacc acgtcgaccc
     3541 tcgacgccct ctgggtcctc tacgaccggg tcctgcgcat cgatcccgcg catccggacg
     3601 ccgaggatcg ggaccggttc atcctgagca agggacatgg tccagcgtcg ctgtacgcga
     3661 tcctggcggc gaaggggttc ttcccgcccg actggctcga cgccttcctc gaccacgacg
     3721 gacgcctcgg ctcgcacccg gaccgtatgc aggtgcccgg catcgaggct tcgaccggct
     3781 cgctcggtca cggattcccg atggcggtgg gcgtggcgca ggcgttgcgg gcgaagggcc
     3841 gccccgacac ccaggtggtc gtcctcacgg gtgatgccga gctcaacgag ggctccaact
     3901 gggaagccgt gatgctggcg cccaacctcc gactgggcaa cctcacctgg ctgatcgtcg
     3961 acaaccacag ctcctcgtgg acgatgggac cgtgggacgc ctggctgccc ccgttcggat
     4021 gggacgtgcg cgtcgtcgac ggtcacgatc atgacgcact ggcggcggcg ctcggggatc
     4081 gtcacgcgga acagccggtg gcggtcgtcg ctgacatccc ggagggggag tggtgatggc
     4141 cgcgatgcgt gaagcgttcg ccgccgcggc cacggacctg ctcgaggccg acgagcgggt
     4201 ggccatcgtg ctcgccgaca tcagcgagac cctgctgagg cgcgcgatcc ggcacgatcc
     4261 ccttcgtgcc gtcaacgtgg ggatcgccga gcagaccatg gtgggcgtcg cggccggatt
     4321 cgccatggag gggttccatc cgatcgcgca ttcgctgtcg ccgttcatgg ccgagcgccc
     4381 ctacgagcaa ttgaaactcg acttcggcta tcagggcctc ggcgggacct tcgtgggctc
     4441 gggcggctcc tacgactacg ccggagaagg cgcgacccat cattcgccgg cggacgcgtc
     4501 gctgatgctc gcgatcccgc gcatgcaggt gctggtgccg ggtcacggac gtgaggtgga
     4561 ccaactgctt cgggcggtgg tggcgaacgg cgagcccacc tacgtgcgca cgagcctggc
     4621 gacgaacgac gaaccccatg acgtggcacc cggccggctc gaggtgatcc gtcgcggcgc
     4681 cggacccacg gtggtggcgt tcgggcctat gctcacgcgc gcgctggagg cgaccgctca
     4741 tctggatgcc acggtggtgt acgccacgag cctcgagccc ttcgatggtc ccacgctccg
     4801 cgagctggcc agagacgccc ccgacatcgt ggtcgtcgag ccctggtacg agggcaccgc
     4861 ggccgccgtg atcacggccg tgttcgccga tgtggcggtt cgcctgcggt ccatcggggt
     4921 tccacgccgg ttcatccatc gttacggcac gtggcaggag ctcgacgcct ccttgggcat
     4981 ggacgccacc ggtatccgag cacgcctgtg acgcaagcga tccggtgacc ctctcaggtc
     5041 cccggagcgg ggcgtcgatg ctccaagcat gcaggaagcg cagcgttcga ccgatgccct
     5101 cgtggacccg gcccagctgt tcgcggccgg tcccgaggcc ctgctcgtcg tgcgcgaagg
     5161 ccggatcgcc tgcgcgaacg aacgtgcact cgatctggtc gggcgcccga tcgctggcct
     5221 cgacctccac gaggtgatcc ctgattggcg cgcggcacct gacgatgccg tgccgttcga
     5281 ggcgatgctc cgctgtgcgg acggcgaact cgcgatcgag gtgcgcgtcc gcgagctcga
     5341 ggacggggtc acggtggcgt cgctgcgaga cgcccgcgcg ctgatcgccg gtcgcgaggc
     5401 cgaagccgcg ctcgccgatg cggaggagcg ctatcgcggc ctggtggaac agatccccgc
     5461 cgtggtctac gccgacgacg gcaacgtcac cacctacgtc aatccgcaga tcgagcacat
     5521 cctgggcgtc acgccgcagg cctatcggga cgatccgaac atgtggctgc gcatggtgca
     5581 tccggacgac cgggagacgg tcgagtcgca gagccaggcc ttcatcgacg gcgtaggggg
     5641 cgatctcgcc gattaccgga tgatccgtcc cgacggcggg atcgtctggg tgcgcgaccg
     5701 tgcatacgcc caccgtgatg acgacggccg cgtgctgtgg gagcacggca tcctgttcga
     5761 cgtcaccgag ctgaaggaag ccgaagcgcg gatcgcgcat atggccttcc acgacggcct
     5821 caccgggctc gccaaccggg agcttttcga ggagacgctg gcgctggccg tgcagcgcgc
     5881 gaagcgcgac gggacggtgg tcgccgtcct gtacctggac ctggacaact tcaagctggt
     5941 gaacgactcg ctcggccacc atgcgggcga ccggctgttg atccagctcg cggaccggct
     6001 cagcgcgtgc acgcgcgaca ccgacctggt ggcgcggcag ggcggcgacg agttcctcat
     6061 gctgctcgcc gacctcggcc acgccgacta cgtcgaggcg atccgggccg tcaccgaccg
     6121 cgtgaacgag gcgatggccg cgccgttcga cctggagggc acggagttct tcgcgcgagg
     6181 ctccatcggc atcagcgtgc atccccacga cgcgcacgac gtcgagaccc tgatgcgcaa
     6241 cgccgacgtc gc
//
LOCUS       DATDBZ010000209         3120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_276904, whole genome shotgun sequence.
ACCESSION   DATDBZ010000209 DATDBZ010000000
VERSION     DATDBZ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276904"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1892)
                     /gene="glmS"
                     /locus_tag="VIX62_05250"
     CDS             complement(60..1892)
                     /gene="glmS"
                     /locus_tag="VIX62_05250"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY5859670.1"
                     /translation="MCGIVGYAGPDEALPIIIEGLRRLEYRGYDSAGIAVLNGGLDVV
                     KRAGKLASLEAALADEGSVTGSIGMGHTRWATHGAPTDRNAHPHQDCSGRVAVIHNGI
                     IENFQGLRARLEKDGHTLTSETDTECIAHVIEEQLAAGATSLPDAVRRTVRELEGAYA
                     LVVCSVDDPEVLVGVRVSSPLVVGIGEGETLLASDASAVLDRTTTVIPVEEGQVVEVR
                     QDGAVFTDFDGLVQHPEPITVDWDVAQAQKGGYDSFMRKEIDEQPAAIRDTLVGRVVG
                     GRLVLDELHISDDVLREVNKVFVVACGTAFHSGMVAKYAIEHWTRLPVEIEIASEFRY
                     RDPVLGPDTLTLAVSQSGETIDTLEAARHARRQGSQVIAVTNTVGSSLAREADGVLYM
                     HAGTEICVAATKTFATQMVSQYLVALYLAQVRGSMFPEEIAEVLDRLDELPDAVQRAI
                     GLDDRCRALAERYVDAPTVLFIGRHTGYPAALEGALKLKELSYLHAEGFPAGELKHGP
                     IALVEAGVPVVAVATTCHIYPKMLSNIQEVRARGAEVIAVATEGDTTIAELADHVLEV
                     PATPELLAPVTVTVPLQLFAYHVATLRGCDVDQPRNLAKSVTVE"
     gene            complement(1946..>3120)
                     /locus_tag="VIX62_05255"
     CDS             complement(1946..>3120)
                     /locus_tag="VIX62_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY5859671.1"
                     /translation="EWLEDALVEGIRHAGGDALIAGVEPTPAIAFLTTTLGASSGVVI
                     SASHNPPEYNGIKFFDRTGTKLADAIEDEIEAVLAGPHDAADRPGQTLPIGDGRERYF
                     AHLVDAAQAPLEGMSVVVDCANGAASELAPALLQRLGATVHAINADPDGKNNHDGCGA
                     LYPEVVAAEVLRVGADAGVSHDGDADRALFADAQGAIVDGDQVLAACAVDLKHRGELP
                     GNTVVTTVMANLGFHHAMREAAIDVVASKVGDRYVLEDMMRIDAPLGGEQSGHVIFRE
                     RSTTGDGLLTAVRFLSLATRTGSSIRQLASVMHRYPQRMINVDVVDRDALEAAEPVWA
                     AVATSESTLGQDGRVLVRASGTEPLVRVMVEAATEDLASSHAESIADAVRTAIGVA"
BASE COUNT          466 a         1102 c         1051 g          501 t
ORIGIN      
        1 accatgatcc ccatcccgat caggatgaag agtcctcctg cagcaagcat ggatgcaccc
       61 tattccacgg tgacgctctt tgcgaggttc cgtggctgat ccacgtcaca tccgcggagc
      121 gtcgccacgt gatacgcgaa gagctgcaag ggaacggtga ccgtgaccgg ggcgagcagt
      181 tccggcgtgg ccggcacctc gagcacgtgg tcggcgagct cggcgatggt ggtatcgcct
      241 tcggtggcca ccgcgatcac ctcggcgccg cgagcgcgga cctcctggat gttggacaac
      301 atcttcgggt agatgtggca ggtcgtggcc accgcgacga ccggcacacc cgcttccacc
      361 agggcgatcg gtccgtgctt gagttcgccc gcggggaagc cttcagcgtg gaggtagctg
      421 agttccttca gcttgagcgc accttcgagc gcggccgggt agccggtgtg tcgcccgatg
      481 aagagcacgg tcggcgcgtc gacgtagcgt tcggccaagg cgcggcatcg atcgtcgagc
      541 ccgatcgcgc gctgcaccgc gtccgggagc tcgtcgagac ggtcgaggac ctcggcgatc
      601 tcctcgggga acatcgagcc ccgcacctgg gccaggtaga gggcaaccag gtattgcgac
      661 accatctgcg tcgcgaaggt cttcgtggcg gcgacgcaga tctcggtgcc agcgtgcatg
      721 tagagcacac cgtcggcctc gcgcgccagc gacgacccca cggtgttcgt caccgcgatc
      781 acctgtgaac cctgccggcg cgcgtgccgt gccgcctcca acgtgtcgat cgtctcgccg
      841 ctctgcgaca ccgccagcgt cagcgtgtcg ggaccaagga ccgggtcgcg gtagcggaac
      901 tcgctcgcga tctcgatctc gacgggcaaa cgggtccagt gctcgatcgc gtacttcgcg
      961 accatgcctg agtggaacgc cgtgccgcag gccacgacga agaccttgtt cacctctcgg
     1021 agcacgtcgt cgctgatgtg gagctcgtcc agcaccagcc ggccaccgac caccctcccc
     1081 accagcgtgt cgcggatcgc ggcgggctgt tcgtcgatct ccttgcgcat gaacgagtcg
     1141 tagccgccct tctgcgcctg ggcgacgtcc cagtccaccg tgatcggctc tgggtgctgc
     1201 accagtccgt cgaagtcggt gaacacggcg ccgtcctggc ggacctcgac gacctgcccc
     1261 tcctcgaccg ggatcacggt cgtggtgcgg tccagcaccg ccgaggcatc ggaagccaac
     1321 agcgtctccc cctcaccgat ccccaccacc aacggggagg acacccggac gccgaccagc
     1381 acctcggggt catcgaccga acacacgacg agcgcgtacg cgccttcgag ctcgcggacg
     1441 gttcgccgca ccgcgtccgg gagcgaggtg gcgccggcgg ccagttgctc ttcgatgacg
     1501 tgggcgatgc actcggtgtc ggtttcggac gtcagcgtgt gtccgtcctt ctccaaccgg
     1561 gcgcggagcc cttggaagtt ctcgatgatg ccgttgtgga tcacggcgac cctgccgctg
     1621 cagtcctgat gcgggtgcgc gttgcggtcg gtcggcgcgc cgtgggtcgc ccaccgcgtg
     1681 tgacccatcc cgatcgaacc cgtgacgctc ccctcgtccg cgagcgcggc ttcgagggag
     1741 gcgagcttgc cggcacgctt gacgacgtcg aggccgccgt tgagcacggc gatgcccgcc
     1801 gagtcgtacc cgcgatactc gagccggcgg agaccttcga tgatgatcgg aagggcttcg
     1861 tcgggccctg cgtagcccac gatgccgcac atgaccgctc aaccctagcg caggggtccg
     1921 tgaacgcccg gagcctcgcg gagcctcagg ccacgccgat ggcggtccgc acggcgtcgg
     1981 cgatcgactc ggcgtggctg ctcgccaggt cctcggtggc cgcctccacc atgacccgga
     2041 ccagcggctc ggtgccggat gcgcgaacca acacgcgacc gtcctgtccc agggtcgact
     2101 cgctcgtggc gacggcggcc cagacgggtt cggcggcctc cagcgcgtcg cgatcgacca
     2161 cgtccacgtt gatcatgcgc tgcgggtacc ggtgcatcac cgatgcgagc tggcggatcg
     2221 acgagcccgt gcgggtcgcc agtgagagga accgcaccgc ggtgagcagg ccgtccccgg
     2281 tggtcgaacg ctcccggaag atcacgtgcc cggactgttc tcccccgagc ggggcgtcga
     2341 tccgcatcat gtcctcgagg acgtagcggt cgcccacctt ggacgccacc acgtcgatcg
     2401 ccgcctcgcg catcgcgtgg tggaacccga ggttggccat caccgtggtg acgaccgtgt
     2461 tgccgggcag ctccccacga tgcttcaggt cgaccgcgca cgccgccagc acctgatccc
     2521 cgtcgacgat cgctccctgg gcgtcggcga acagcgcgcg atcggcatcc ccgtcgtggc
     2581 tcaccccggc gtcggcgccc acgcggagga cctctgccgc cacgacctcc gggtacaggg
     2641 ccccgcaccc gtcgtggttg ttcttgccgt cgggatcggc gttgatcgcg tgcaccgtgg
     2701 cgccgagccg ctggagcagc gcgggagcga gttcggaagc ggcaccgttg gcgcagtcca
     2761 ccacgacgct catcccctcc agcggggcct gggcggcgtc gacgaggtga gcgaagtagc
     2821 gctcgcgccc gtcgccgatc ggcaacgtct ggccgggacg atcagcggca tcgtgcggcc
     2881 cggccagcac ggcttcgatc tcatcctcga tcgcgtcggc cagcttcgtc ccggtccggt
     2941 cgaagaactt gatgccgttg tattcgggcg ggttgtggga cgccgagatc accacccccg
     3001 atgaagcgcc cagcgtcgtg gtgaggaacg cgatcgcggg ggtgggctcg acgccggcga
     3061 tcagggcgtc gccgcccgcg tgccggatgc cttcgaccaa tgcgtcctcg agccattcgc
//
LOCUS       DATDBZ010000210         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1294955, whole genome shotgun sequence.
ACCESSION   DATDBZ010000210 DATDBZ010000000
VERSION     DATDBZ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294955"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /gene="purE"
                     /locus_tag="VIX62_05260"
     CDS             complement(<1..363)
                     /gene="purE"
                     /locus_tag="VIX62_05260"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY5859672.1"
                     /translation="MQPAPLSHDSGVPLVGVVMGSSSDWGTMRHASELLTEFGIPHET
                     RVVSAHRTPEAMYDYARSARERGVRCIIAGAGGAAHLPGMLAASTTVPVLGVPVPSRH
                     LQGLDSLYSIVQMPKGVPV"
     gene            407..1735
                     /locus_tag="VIX62_05265"
     CDS             407..1735
                     /locus_tag="VIX62_05265"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859673.1"
                     /translation="MTVPTAAPLSHPFTPGSVRAAFSYRDFRLVWIGLLASNIGTWMQ
                     IVALPAYVQRRTGSAAAVGLMVFAQLGPLLLLSIPGGILANRFPRRPWLITMQSAQIV
                     FSLGLAALVAQHAPLAALFGVQLLIGIANALNAPALQASIPLLLDRRDLPGAIALNSA
                     SLNGSRVIGPVIAALLGLLGVTVSQLFVINAATYLFVIAALAVVHIPVIVDRYGDQGW
                     RQLTVGVRIARSRPILARILLSMALFSFFSLPYVGLFPAVAELNFGIDAGSPAYKWLY
                     ATWGLGACLGALGTGTLFAGVDKRRLITPGFLGFAVSLAAFALVSVPAPAFPIGFVLG
                     FCYFLTATGLLTVLQESIEDHERAVVMSLWFMAFGGTVPIGNLVFGPLMDHIGARWIM
                     LGGALFAVFLAWWCNLDAPRWRRSGEQPRRKPLEPGDPAALDEYGVVAGD"
     gene            complement(1652..>2161)
                     /locus_tag="VIX62_05270"
     CDS             complement(1652..>2161)
                     /locus_tag="VIX62_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY5859674.1"
                     /translation="RVEVELPVSGRHMVGNACAAAAIGVVLGVPLDGIAAGLAGAALS
                     PWRMEISVAPSGAVVINDAYNANPTSMRAALDALASVPARRHVAALGVMAEIADADDE
                     HRAIAAYAAERGIELVPVGTDLYGMPSAGDASVVAVSIIGSIAGDDAVLVKGSRVAGL
                     ERLAARLLA"
BASE COUNT          303 a          773 c          712 g          373 t
ORIGIN      
        1 caccggaacg cccttcggca tctgaacgat ggagtacagc gaatccagcc cctggaggtg
       61 gcgggacggc acgggcacgc ccagcaccgg caccgtcgtc gaggcggcga gcattcccgg
      121 caggtgggca gctccgccgg cgccggcgat gatgcaacga acgccacgct cccgagccga
      181 gcgggcgtag tcgtacatcg cctcgggcgt gcgatgggcg gacaccaccc gggtctcgtg
      241 agggatgccg aactcggtga ggagctcgct ggcgtgacgc atcgtccccc agtcggacga
      301 cgatcccatg acgaccccga cgagtggcac accggaatca tgggacaacg gtgccggctg
      361 catggttcaa tcgtaggaac ttgaccgacg ggtcaagcgt attgccatga ccgttcccac
      421 cgcagcccct ctctcccatc cgttcacgcc cggctccgta cgggcggcgt tcagctaccg
      481 cgacttccgg ctggtctgga tcggcctgct ggcatccaac atcggcacgt ggatgcagat
      541 tgtcgccctc ccggcctacg tgcagcggcg cacgggttcg gcagccgccg tcgggctgat
      601 ggtgttcgcc cagctcggcc cactcctgct gctgtcgatc cccggtggca tcctggccaa
      661 ccgcttccct cgccgaccct ggttgatcac gatgcagagc gcgcagatcg tgttctcgct
      721 cggcctcgca gcactggttg ctcagcacgc accacttgca gcgctgttcg gcgtccagct
      781 gctcatcggc attgcgaatg cgctcaacgc gcccgcgctc caggcgtcga tcccgctcct
      841 cctcgaccgg cgcgacctcc ctggcgcaat cgcgctcaac tcggccagcc tgaacgggtc
      901 acgggtgatc ggaccggtca tcgccgcgtt gctcggactc ctgggcgtca cggtgtcgca
      961 gctgttcgtg atcaacgcgg cgacgtacct gttcgtcatc gccgcactgg cggtggtgca
     1021 catcccggtc atcgtcgatc ggtacggcga ccagggatgg cggcagctga cggtgggcgt
     1081 cagaatcgcc cgcagccgcc cgatcctcgc tcggatcctg ctctcgatgg cgctgttctc
     1141 gttcttctcg ctgccctatg tcggcctgtt tcctgcggtg gccgaactga acttcgggat
     1201 cgacgccggc agtcccgcct acaagtggct ctacgcgacg tgggggctcg gtgcctgcct
     1261 gggtgcgctc ggcaccggga cgctcttcgc cggtgtggac aagcggcgcc tgatcacgcc
     1321 cggattcctc gggttcgctg tcagcctcgc tgcgttcgca ctcgtcagtg tgcccgcgcc
     1381 cgcgttcccg atcggcttcg tactcggctt ctgctacttc ctcaccgcca cagggctgct
     1441 caccgtgcta caggagagca tcgaggacca cgaacgggcg gtcgtgatgt cgctgtggtt
     1501 catggcgttc ggcggaaccg tcccgatcgg caaccttgtc ttcggccccc tcatggacca
     1561 catcggggca cgctggatca tgctcggcgg tgcgctgttc gcggtcttcc tcgcctggtg
     1621 gtgcaacctc gacgcgcccc ggtggcggcg ctcaggcgag cagccgcgcc gcaagccgct
     1681 cgagcccggc gaccctgctg cccttgacga gtacggcgtc gtcgccggcg attgagccga
     1741 tgatcgagac agcgacgacc gacgcgtccc cggcggaggg catgccgtag agatcggtgc
     1801 ccaccggaac gagttcgatc ccccgctcgg ccgcgtaggc ggcgatcgcc cggtgttcgt
     1861 catcggcgtc ggcgatctcc gccatcacgc cgagcgcggc gacgtggcgc cgggcgggca
     1921 ccgaggcgag tgcgtcgagc gccgcccgca tcgaagtcgg gttggcgttg tatgcgtcgt
     1981 tgatcaccac tgcgcccgag ggagcgacgc tgatctccat ccgccacggc gagagcgccg
     2041 ccccggcgag accggcggcg atgccgtcga gcgggacgcc gagcacgacc ccgatcgccg
     2101 ctgccgcaca ggcgttgccg accatgtggc ggccgctcac ggggagctcc acctcgaccc
     2161 g
//
LOCUS       DATDBZ010000211         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_478742, whole genome shotgun sequence.
ACCESSION   DATDBZ010000211 DATDBZ010000000
VERSION     DATDBZ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478742"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VIX62_05275"
     CDS             complement(<1..515)
                     /locus_tag="VIX62_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY5859675.1"
                     /translation="MSYGGAGVPEIEPDLGPPEGSRRSRILGDRDGGRNVWVAVASTI
                     IFFAVLITLIVNSPGWSAPGGVRDAFFNKELFLESLPKILDGFWLNVRIFLTAEILVL
                     MFALVLAVMRGLPGPVFAPLRLLAVSYSILFRGVPTILVIFILGFGMPALELQGVPSS
                     DVFWAIVALTLV"
     gene            complement(546..1445)
                     /locus_tag="VIX62_05280"
     CDS             complement(546..1445)
                     /locus_tag="VIX62_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5859676.1"
                     /translation="MRKLMIGLAAFAIIAAACAPEDDTPAASDSTGEAPATAEECVQG
                     ADIPLTTEGALTIGTGNPAYAPWYGGKSVEGSEWESSSFTGDPHSGEGYESAFAYALA
                     GTMGFDDDAVEWVGIPFAKSFAPGPRDFDFAMQQISYSDKRAEAVDFSDGYFDVNQAL
                     IANNDSPAVGVTTLDGLKDLKLGAPIGTTSLEAIEELVQPNVEPSIYNNLDLALKDLK
                     NGQVDGVVVDYPTAFYGYADVVVGQFPPVGGEQEYFGVTFEKANPLVECVNIGIAELE
                     DDGTLQELQDKWLTTDVDVPVFS"
     gene            complement(1548..2318)
                     /locus_tag="VIX62_05285"
     CDS             complement(1548..2318)
                     /locus_tag="VIX62_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859677.1"
                     /translation="MSEAAQVLRQVRYTNKAFWRNPASAFFTFAFPLMFLVIFTSLLS
                     NGQITIGNQEIDLSTYYVMAEAAFGVISACFTNIAISVTFQRDQGILKRTRGTPLPGW
                     AYLTARVVHAMTIAFVLVAITVAFGVIFYSASVPTGIPLVQFVLTLLVGGLSFAALAL
                     ALTAAIPNADAAPPIVNAAVLPLLFLSGIFFPTDDAPTWITAVANIFPVRHFFEAMSS
                     AFLGNAIPFDWWDLVVVAAWGIVGLVLASRFFSWEPRK"
     gene            complement(2311..3222)
                     /locus_tag="VIX62_05290"
     CDS             complement(2311..3222)
                     /locus_tag="VIX62_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5859678.1"
                     /translation="MGDRVIDVEGLRKSYGDVDAVRGIDLHVDRGEIFALLGPNGAGK
                     TTTVEILEGFRTATAGRAQVLGHDPGRHEPALAERIGIVLQSTGVDPYLTVQETVELY
                     SGYYPSPRPAAEVIDMVGLGSKADSRVLKLSGGQQRRLDVAIALAGDPELLFLDEPTT
                     GFDPSARHAAWDIIKSLTELGKTVFLTTHFMDEAQYLADRLAVLAHGQIVAEGTPSTI
                     AGRDRMLARIQFRIGGAQDGLPALGQERGVDGTWTIRAEDPTRPLHELTGWAIERGVV
                     FDALEVTRPSLEDVYLEITGGEAEAGE"
     gene            complement(3285..>3466)
                     /locus_tag="VIX62_05295"
     CDS             complement(3285..>3466)
                     /locus_tag="VIX62_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY5859679.1"
                     /translation="PADSPLWAMPQVIVSPHVSGDVEGWERAVVQVFTDNAARWVRGE
                     PLVNLIDKRAGHGTS"
BASE COUNT          599 a         1150 c         1170 g          547 t
ORIGIN      
        1 acgagcgtca gggcgacgat ggcccagaac acgtcgctgc tgggtacccc ttgaagctcc
       61 agcgccggca tgccgaatcc gaggatgaag atgaccagga tggtcggcac gcctcggaac
      121 aggatcgagt acgacacggc cagcaacctg agcggtgcga agacgggacc ggggagaccg
      181 cgcatcactg ccaacacgag ggcgaacatc aggaccaaga tctcggcggt caggaagatc
      241 cgtacgttga gccagaagcc atccaggatc ttcggcaggg actcgaggaa gagttccttg
      301 ttgaagaacg cgtccctcac cccacccggc gccgaccacc ccggggagtt gacgatcagg
      361 gtgatcagga ccgcgaagaa gatgatcgtg ctggcgaccg cgacccagac gttccgcccg
      421 ccatcgcggt cgcccaggat gcgagacctg cgactgcctt cgggaggccc gaggtcaggt
      481 tcgatctcgg gaacgcctgc ccctccgtac gacacgggcg atccccgttc ctcggtggtc
      541 gatggctacg agaagaccgg gacgtcgacg tcggtggtga gccacttgtc ctggagttcc
      601 tggagcgtgc cgtcgtcctc cagctccgcg atcccgatgt tgacgcactc caccaacggg
      661 ttcgccttct cgaacgtcac gccgaagtac tcctgctcgc cgcccaccgg cgggaactgg
      721 cccacgacca cgtcggcgta gccgtagaac gccgtcgggt agtcgaccac cacgccgtcg
      781 acctggccgt tcttgaggtc cttcagcgcg aggtcgaggt tgttgtagat gctgggctcg
      841 acgttcggtt gcacgagttc ctcgatggcc tccaggctcg tggtgccgat cggggcgccg
      901 agcttcaggt ccttgaggcc gtccagggtg gtgacgccga ccgcggggga atcgttgttc
      961 gcgatcaagg cctggttcac gtcgaagtag ccgtcgctga agtccaccgc ttcggcgcgc
     1021 ttgtcggagt aggagatctg ctgcatggcg aagtcgaagt cccgtggtcc cggcgcgaac
     1081 gacttggcga acgggatgcc cacccactcg accgcgtcgt cgtcgaaacc catcgtgccg
     1141 gccagggcgt aggcgaacgc gctctcgtag ccctcaccgc tgtgcggatc gcccgtgaag
     1201 gagctcgact cccattcgga accctcgacg ctcttgccgc cgtaccaggg cgcgtacgcc
     1261 gggttgcccg tgccgatcgt gagcgcgccc tcggtggtga gcgggatgtc ggcgccctgc
     1321 acgcactcct cggcggtggc cggagcctcg ccggtgctat cggacgcggc gggtgtgtcg
     1381 tcctcggggg cacatgcggc ggcgatgatc gcgaaggccg ccaggccgat catgagcttc
     1441 cgcatcgtgc ataccccctg ttgctgcgtg gactggacga gtgggaacga gctgtaagcc
     1501 ccgctcgcgc cggagcctag cagagtgatt tcgagccgtg gaggcagcta cttccgaggt
     1561 tcccaactga agaaccggga cgcgagcacc aggccgacga ttccccacgc cgccacgacg
     1621 acaaggtccc accagtcgaa cgggatggcg ttgccgagga acgcactgga catggcctcg
     1681 aagaagtgcc gcaccgggaa gatgttggcg acggccgtga tccacgtcgg cgcgtcgtcg
     1741 gtcgggaaga agatccccga gaggaagagc agcgggagca ctgctgcatt gacgatcggc
     1801 ggggccgcgt ccgcgttggg gatcgcggcg gtgagggcca gcgccagcgc cgcgaagctc
     1861 aagccaccca ccagcaacgt caggacgaac tgcaccaacg ggatgcccgt gggcacggac
     1921 gccgagtaaa agatcacgcc gaatgcgacc gtgatggcca ccaggacgaa ggcgatcgtc
     1981 atcgcgtgga cgacccgggc ggtgaggtag gcccaccccg gcagcggggt cccacgcgtc
     2041 cgtttgagga tcccctggtc gcgctggaag gtgacgctga tggcgatgtt cgtgaagcac
     2101 gcggagatca cgccgaacgc ggcttcggcc atcacgtagt aggtcgagag gtcgatctcc
     2161 tggttgccga tcgtgatctg cccgttgctc agcagcgacg tgaagatcac caggaacatc
     2221 agtgggaacg cgaacgtgaa gaacgccgac gcggggttcc gccagaaggc cttgttcgtg
     2281 tatcggacct gccgcagaac ctgggcggcc tcactcaccg gcttccgcct ccccaccggt
     2341 gatctcgaga tacacgtcct cgagactcgg acgcgtgacc tcgagcgcgt cgaacacgac
     2401 accgcgctcg atcgcccatc ccgtgagctc gtgcaacggc cgtgtcggat cctccgcgcg
     2461 gatcgtccac gtgccgtcca cgcctcgctc ctgccccagg gcggggagac cgtcctgcgc
     2521 cccgccgatg cggaactgga tccgcgccag catgcgatcg cggccagcga tcgtcgaagg
     2581 cgtgccctcg gcgacgatct gcccgtgcgc cagcacggcg agccgatccg cgaggtattg
     2641 cgcctcgtcc atgaagtgcg tcgtgaggaa gacggtcttt ccgagttcgg tgaggctctt
     2701 gatgatgtcc cacgcggcgt ggcgtgcgct tgggtcgaaa cccgttgtcg gctcgtcgag
     2761 gaacaggagt tcgggatcgc ccgcgagcgc gatcgcgacg tccaggcgac gttgctgccc
     2821 gccggagagc ttcagcaccc gagagtcggc cttcgagccc aggccgacca tgtcgatcac
     2881 ctccgccgcc gggcgggggc tcgggtagta gccgctgtaa agctcgaccg tctcctgcac
     2941 cgtcaggtag ggatccaccc cggtcgactg caggacgatg ccgatccgtt ccgcgagcgc
     3001 cggctcgtga cggccgggat cgtggccgag cacttgcgcg cgaccagcgg tcgccgtccg
     3061 gaagccttcg aggatctcca cggtggtggt cttgcccgcg ccgttgggtc cgagcagggc
     3121 gaagatctcg ccgcggtcga cgtgcaggtc gatcccgcgg accgcgtcga cgtcaccgta
     3181 cgacttgcgc aacccctcga cgtcgatcac ccggtcgccc acggcgtcag gcttgggcgg
     3241 gacatctcga gcgacaaggt ccgcccgccg ggctcgatcc gggatcagga cgtaccgtgg
     3301 cccgcgcgct tgtcgatcag gttgaccaat ggctcgccac gcacccaccg agcggcgttg
     3361 tcggtgaaca cctgcacgac ggcacgctcc cacccctcga catcgccgct cacatgcggc
     3421 gagacgatca cctggggcat ggcccacaag gggctgtcgg ccggga
//
LOCUS       DATDBZ010000212         4946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2526936, whole genome shotgun sequence.
ACCESSION   DATDBZ010000212 DATDBZ010000000
VERSION     DATDBZ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2526936"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..685
                     /locus_tag="VIX62_05300"
     CDS             242..685
                     /locus_tag="VIX62_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY5859680.1"
                     /translation="MLDARDLDIIAALQDDARATYADIAAKVGMSASAVHERVRKLEQ
                     QQVIQGYTAVVDPQALGLLVTAIIAASPLDPTQPDDLPDRVAEFPEVQDCFSVAGETN
                     YVLKVRTRTTADLEDLIRRLREKGNVATRTTIALSIPFEGRPLRT"
     gene            721..2112
                     /gene="cysS"
                     /locus_tag="VIX62_05305"
     CDS             721..2112
                     /gene="cysS"
                     /locus_tag="VIX62_05305"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY5859681.1"
                     /translation="MRLYNTLTRRIDEVVPIEEGHVTMYTCGPTVYRYAHLGNLRSQM
                     LADLIRRALMFEGFRVTQIMNITDVGHMTDEASPEAVDKMLLAVEDEGLSPLEIAEKY
                     TAAVFEEAEAVGIAPADRYPKATDHIQEMVDLTQVLIDRGHAYVVDSGSVYYDVTSFP
                     GYGKLSGNTLDKLREGHRDLETDPAKRNVADFALWKAAGAGRLMKWDSPWGEGFPGWH
                     IECSAMSMKYLGDRFDIHTGGNDLRFPHHEDEIAQSEGAVGHEVVSIWVHAGHLRQSG
                     QKISKSTGNTVRVPDLIERGFDPLAFRWLAFQTQYRSEMDFSWDAMEVADQRVRHLRR
                     RYAAWGEPATELGEAAKGFDARFREEIANDLHMPGAVAVVNELASSADVPDPEKAALL
                     ASWDQVLGIDVGREARTTWEPTAAMRELMAQRDAARAAKDYATSDRLRDELAALGVEV
                     MDTAAGTEVRPLD"
     gene            complement(2136..3233)
                     /locus_tag="VIX62_05310"
     CDS             complement(2136..3233)
                     /locus_tag="VIX62_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5859682.1"
                     /translation="MNERLNDRLRRATAEATAQGYAAIIVAPSPDLAYLTGYEPMPME
                     RPTLLVLRDDRDPVMLVPELERPLAAASSAGAVVELVGWTDGDDPYTAAADLLEPRGR
                     VAVGDRLWASHVLGLQGVLPDLTFEPASPVIGRLRAVKDDDELAALRRAARGADETFR
                     QICSSTFLGRREEEIAADLADLLVQHGHAYADFTIVASGPNSASPHHEPSGRTILPRD
                     VVVMDFGGELGGYFSDTTRTVVVGEPPKAFERVYDTVRRAQEAACEAVRPGVSCQDVD
                     RTARRIIDDAGFGARFIHRTGHGIGREVHEPPYIVEGNEWVLEPGTTFSVEPGVYLED
                     SFGVRIEDIVAVTDDGVERLNRSTRELKVVS"
     gene            complement(3268..4197)
                     /locus_tag="VIX62_05315"
     CDS             complement(3268..4197)
                     /locus_tag="VIX62_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY5859683.1"
                     /translation="MTSPFGTLAVIADPADDDGRVAAELPALERALGEGDLDYRLTLA
                     DPGRGLGSLASAALDDGARFVVAVGSDATVQDVVNGMFRDGRPIVDEAVLGVVAANTG
                     NQLVRSFGLPGDTEGGVRRLAGADTYPFDVMKVTCAGPEGPVTRYAANLAMMGLAARV
                     ELRRQRMPGWLGERGRAFASFWRGYIGAHRQQVSIAYDTKRWDGETFQIIVGNAQYGP
                     DGLRLSPRSWPGDGVLDALVFTGQKSDAYTMMPRIMRHGDHIPDPNIHELRAKIRFAV
                     DAECPMTVVADGREFGTTPATFQVVAHQLLFKL"
     gene            complement(4215..>4946)
                     /locus_tag="VIX62_05320"
     CDS             complement(4215..>4946)
                     /locus_tag="VIX62_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEY5859684.1"
                     /translation="DPKVEREAARLEALAAEHPCASCPERAKHERWATRVDEKRRSLR
                     GVERRIRVRTETLARQFDRVLEVLRTLGFVEDWRVTDKGRTLARIYGEADILIGEALS
                     KGVFDDLAPAEVAALLSSAVYEGRERVPLSGEMPTEATVERYERFQRLWRQIRRTEDE
                     HQVQLCRELEQGFATPIYHWAQGLPLEAVLAETEMAPGDFVRNCKQLLDLLRQVEEVA
                     QPDVAALFAAARTAVLRGVVAYTGV"
BASE COUNT          774 a         1754 c         1677 g          741 t
ORIGIN      
        1 atcggcgggc cgaggggggc gggtcgaacc caccgcgagc gtgcgggcgt ggccatacga
       61 gcggtcctcc atcgttcgtg tgcggatcgc cgagggagga gggcgccgtc gcaccggttc
      121 taccgaggac cgcccaccgg cgcaatcggc cggcgatcgc gaacgccgtc ccccgagccc
      181 atcccccggc gggtcggatg acgcgggcgg gggccgatgg gcggggacct atgatgggcg
      241 catgctggat gcgcgagatc tcgacatcat cgccgcgttg caggacgacg cgcgggccac
      301 ctacgccgat atcgcggcga aggtgggcat gtcggcctcc gcggtgcacg agcgcgtccg
      361 caaactcgaa cagcagcagg tgatccaggg ctacacggcg gtggtcgatc cccaggcgct
      421 cggtctgttg gtgacggcca tcatcgccgc ctcgcccctc gaccccacgc agcccgacga
      481 tctcccggac cgggtggccg agttcccgga ggttcaggac tgcttcagcg tcgcgggcga
      541 gaccaactac gtcctgaagg tccggacccg caccaccgcg gacctcgagg atctgatccg
      601 gcgcctgcgc gagaagggca acgtcgccac ccgcaccacg atcgcgttgt cgatcccctt
      661 cgagggccgc ccgctccgga cctgacaggg ctgcgcgaac cctgcagcta tattggcccc
      721 atgcggctgt acaacacgct gacccgccgg atcgacgagg tcgtgcccat cgaagagggc
      781 cacgtcacga tgtacacgtg cggcccaacg gtgtatcggt acgcccacct ggggaacctg
      841 cggagccaga tgctggcgga cctgatccgc cgtgccctga tgttcgaagg cttccgcgtc
      901 acccagatca tgaacatcac cgacgtgggg cacatgacgg acgaagcctc gcccgaagcg
      961 gtcgacaaga tgctgctcgc cgtcgaggac gaaggtctca gcccgctcga gatcgcggag
     1021 aagtacaccg cggccgtctt cgaggaggcc gaggccgtgg ggatcgcgcc cgccgatcgc
     1081 tacccgaagg ccaccgacca catccaggag atggtcgatc tcacgcaggt cctgatcgat
     1141 cggggccacg cctacgtggt cgactccggc agcgtctact acgacgtcac ctccttcccg
     1201 ggctacggca agctcagtgg caacacgttg gacaagctcc gggagggcca tcgcgacctg
     1261 gagaccgacc cggccaagcg caacgtcgcc gacttcgcgc tgtggaaggc ggcgggtgcc
     1321 ggacggctca tgaaatggga ctccccgtgg ggcgaaggtt tcccgggctg gcacatcgag
     1381 tgctcggcga tgtccatgaa gtacctgggc gaccggttcg acatccacac cgggggcaac
     1441 gacctccggt tcccccacca cgaggacgag atcgcgcaga gcgaaggcgc ggtgggacac
     1501 gaggtcgtgt cgatctgggt gcacgcgggc cacctgcggc aatcgggaca gaagatctcg
     1561 aagtccaccg ggaacacggt ccgtgtgccg gatctgatcg agcgtgggtt cgatccgctc
     1621 gcgttccgct ggctggcctt ccagacgcag taccggtcgg agatggactt ctcgtgggat
     1681 gcgatggagg ttgccgacca gcgcgtgcga cacctgcggc gccggtacgc cgcatggggc
     1741 gaacccgcga ccgaactcgg cgaggccgcg aagggcttcg acgcccggtt ccgcgaggag
     1801 atcgccaacg acctgcacat gcccggcgcc gtcgccgtgg tgaacgaact ggcgtcatcg
     1861 gccgacgtcc ccgatcccga gaaggcagcg ctgctcgcct catgggacca ggtgctcggg
     1921 atcgacgtgg gccgtgaagc ccgcaccacc tgggagccca cggcggcgat gcgcgaactc
     1981 atggcgcagc gcgatgccgc cagggccgcg aaggactacg ccaccagcga tcggttgcgc
     2041 gacgaactgg ccgcgctggg tgtcgaggtc atggacaccg ccgccggcac cgaggtccga
     2101 ccgctcgact gacatctcac gacctggtcg tcgagttagc tgacgacctt cagctcccgt
     2161 gtggatcgat tgaggcgttc gaccccgtcg tcggtcaccg ccacgatgtc ctcgatccgc
     2221 accccgaagg agtcctcgag gtagacgccg ggctccaccg agaacgtggt gcctggctcg
     2281 agcacccact cgttgccctc cacgatgtac ggcggctcgt gcacctcccg gccgatcccg
     2341 tgccccgtcc gatggatgaa ccgcgcgccg aaccccgcgt cgtcgatgat ccgccgggcc
     2401 gtgcggtcga cgtcctggca ggacacaccc ggtcgcacgg cctcgcaggc cgcctcctga
     2461 gcgcgacgca cggtgtcgta gacgcgttcg aacgccttcg gtggctcccc caccacgacc
     2521 gtcctcgtgg tgtcgctgaa gtagccgccc agttccccgc cgaagtccat caccacgacg
     2581 tcccggggca ggatcgtgcg gccgctgggc tcgtgatgcg gtgaggcgga attcggtccg
     2641 ctcgccacga tggtgaagtc ggcgtaggcg tggccgtgct ggacgaggag atcggcgagg
     2701 tcggcggcga tctcctcctc gcgacggccc aggaaggtcg aggaacagat ctgtcggaag
     2761 gtctcgtcgg cgccacgcgc ggcccggcgc agtgccgcga gctcgtcgtc gtccttcacg
     2821 gcccgcagcc ggccgatcac cggcgacgcg ggctcgaacg tcaggtcggg cagcacgcct
     2881 tgcagtccga ggacgtgcga ggcccacagg cggtcgccca ccgcgacccg gccccgtggt
     2941 tcgagcagat cggccgccgc ggtgtagggg tcgtcgccgt cggtccaacc caccagttcc
     3001 acaaccgctc cggccgacga cgctgcggcg agcggccgtt cgagctcggg caccagcatc
     3061 acggggtcgc gatcgtcccg gagcacgagc agggtggggc gctccatcgg catcggctcg
     3121 tagccggtga ggtaggccag gtccggtgag ggcgccacga tgatcgcggc gtagccttgc
     3181 gcggtcgcct cggccgtggc ccgtcgcaac cggtcgttca gacgttcgtt catgggcctt
     3241 cctcgttcgt cacgtcgctg catcccctca caacttgaac agcaactggt gggcgaccac
     3301 ctggaaggtg gcgggcgtgg tgccgaactc gcgcccgtcg gccaccaccg tcatggggca
     3361 ctcggcgtcg accgcgaacc ggatcttcgc tcgcagttcg tggatgttgg ggtccgggat
     3421 gtggtcaccg tgccgcatga tcctgggcat catcgtgtag gcgtcggact tctgtccggt
     3481 gaagaccaat gcatcgagca caccgtcgcc gggccacgac cggggagaga ggcgcaaccc
     3541 gtcaggcccg tattgcgcgt tgcccacgat gatctggaag gtctcgccgt cccagcgctt
     3601 cgtgtcgtag gcgatcgaga cctgctggcg gtgggcgccg atgtaaccgc gccagaagct
     3661 cgcgaacgcc cgcccgcgct cgccgagcca tccgggcatc cgttggcgcc gcagttcgac
     3721 ccgtgctgcc agtcccatca tggccaggtt cgcggcgtag cgggtcacgg gtccctcggg
     3781 gccggcgcag gtcaccttca tgacgtcgaa cggataggtg tccgcaccgg cgagccgccg
     3841 cacaccgcct tcggtgtcgc cgggcagccc gaagctccgg accagctggt tgcccgtgtt
     3901 cgccgccacg acaccgagca ccgcttcgtc cacgatcggc cggccgtcgc ggaacatgcc
     3961 gttcacgacg tcttggacgg tggcgtcact ccccaccgcc accacgaacc gcgcaccgtc
     4021 atcgagcgcc gccgacgcga gagatccgag ccctcggccc ggatcggcga gggtgagccg
     4081 gtagtccaga tccccttccc ccagcgcacg ctccagggcg gggagttcgg cggcgacccg
     4141 tccgtcatcg tcggcggggt cggcgatcac ggcgagggtt ccgaaggggc tggtcacgtc
     4201 gccgggcatc gtagctagac gcccgtgtag gcgacgacgc cacgcaggac cgccgtgcgc
     4261 gccgcagcga acagcgcggc gacatccggc tgcgccacct cctccacctg tcgcagcagg
     4321 tcgaggagtt gcttgcagtt ccggacgaaa tcccccggcg ccatctccgt ctccgccagg
     4381 accgcctcca agggcaggcc ctgtgcccag tggtagatcg gggtggcgaa gccctgctcg
     4441 agctcgcggc acagctgcac ctggtgctcg tcctccgtgc ggcggatctg ccgccacagc
     4501 cgttggaacc gctcgtatcg ctccacggtg gcctcggtcg gcatctcccc tgacaacggc
     4561 acgcgttcgc ggccctcgta gaccgccgac gacagcagcg ccgccacctc ggccggggcg
     4621 aggtcgtcga acacgccctt ggacagcgct tcgccgatca ggatgtcggc ctcgccgtag
     4681 atcctggcca gcgttcgccc cttgtccgtc acccgccagt cctccacgaa gccgagcgtc
     4741 cgcagcacct cgagcacgcg gtcgaactgg cgggccagcg tctcggtgcg cacgcggatc
     4801 ctgcgctcga ccccccggag cgagcggcgc ttctcatcga cccgtgtcgc ccaccgttcg
     4861 tgcttggccc gctcagggca cgatgcgcat ggatgctcgg cggcgagcgc ttccagccgg
     4921 gcggcctcac gttcaacctt cggatc
//
LOCUS       DATDBZ010000213         2615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2731480, whole genome shotgun sequence.
ACCESSION   DATDBZ010000213 DATDBZ010000000
VERSION     DATDBZ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2731480"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..2400)
                     /locus_tag="VIX62_05325"
     CDS             complement(43..2400)
                     /locus_tag="VIX62_05325"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012396478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY5859685.1"
                     /translation="MIGRQNLPVPDAHQPYPGLVTYDAKDPATSFAPTEELRPPAGAP
                     NVLVVLLDDVGFAASSAFGGPCDTPVAERLAAGGLKYTRFHTTALCAPTRQALLTGRN
                     HHAVGMGCITELATSAPGYTGIRPNTAAPLADILKLNGYSTSQYGKCHEVPVHQSSPM
                     GPFDAWPTGGGGFEYFYGFISGETNQYYPAIYEGTTPLEPPKTPEEGYHFTEDMTDKA
                     ISWMRQQKSLMPDRPFFAYFAPGATHAPHHVPKDWIERYRGRFDAGWDALREEIFARQ
                     KALGVIPADAELTARPAEIPAWDDMADELKPVLARQMEVYAAFLAHTDHHIGRLIDAI
                     DGLGVLEDTLVYYIIGDNGASAEGGINGTFNETFAFNGAAFLETAEFMSSKIDQFGGP
                     EAYNHFAVGWAHAMDTPYQWTKQVASHWGGTRNGTIVHWPNGFTARGESRSQFCHVID
                     IAPTVLEAAGLPAPSFVHGVQQMPHHGTSMMYSFDDAEAPERHDLQYFEMFVNRGVYH
                     QGWAAVTRHSTPWLPEPPPAIENDAWELYGPDDWTQSHDVAANNPAKLAELQRLFLIE
                     ATKYNVLPLDDRRVERFNADLAGRPVLIKGKTQLLFGGMGRLSENSVLTTKNKSHAVT
                     ANVTVTEAGAEGVIIAQGGAFAGWCMYAKDGIPKYCYNLLGLERFTIVGDQPLPPGDH
                     QVRAEFAYDGGGLAKGATVTLYVDGGKVGDGHLPATVPMVFSGDETTDVGRDNGTGVS
                     DEYRPGDNEFTGTVHWVQIDIDDSAEDFDHLITPEERWQIAMARQ"
BASE COUNT          395 a          821 c          920 g          479 t
ORIGIN      
        1 tgggccgggg ccgatggcac gccagtggcc ggcgtgccgc gcctactggc gtgccatcgc
       61 gatctgccag cgttcctcgg gggtgatcag gtggtcgaag tcttcggcgc tgtcgtcgat
      121 gtcgatctgg acccagtgca ccgtgcccgt gaactcgttg tcgccgggcc ggtactcgtc
      181 actgacaccg gtgccgttgt cgcggccgac atcggtcgtc tcgtcaccgg agaagaccat
      241 cgggacggtt gcggggaggt gaccgtcacc gaccttgccg ccgtcgacgt agagcgtgac
      301 ggtcgcgccc ttggcgagac cgccaccgtc ataggcgaac tccgcgcgca cctggtggtc
      361 gccaggaggc agaggctggt cgccgacgat ggtgaagcgc tcgagcccga gcaggttgta
      421 gcagtacttc gggatcccat ccttggcgta catgcaccag ccggcgaacg cgccgccctg
      481 ggcgatgatc acgccctcgg caccagcctc ggtcacggtg acgttcgcgg tgacggcgtg
      541 tgacttgttc ttggtcgtca gcacggagtt ctcgctgaga cgtcccattc caccgaagag
      601 cagctgggtc ttgcctttga tcaggacggg ccgacccgcg aggtcggcgt tgaatcgttc
      661 gacgcggcgg tcgtcgaggg ggaggacgtt gtacttggtc gcctcgatga ggaacagacg
      721 ctggagctcg gcgagcttgg ccgggttatt ggcggcgaca tcgtgggatt gcgtccagtc
      781 gtcggggccg tagagctccc aggcgtcgtt ctcgatcgcg ggcggtggtt cgggcagcca
      841 cggtgtgctg tgacgcgtca ctgctgccca tccctggtgg tagacgccgc ggttgacgaa
      901 catctcgaag tactgcaggt cgtggcgctc cggagcctcg gcatcgtcga aggagtacat
      961 catgctcgtg ccgtggtgag gcatctgctg gacgccatgc acgaaggatg gcgcggggag
     1021 tcccgcggct tcgagaacgg tcggggcgat gtcgatgaca tgacagaact gcgacctcga
     1081 ctcgccgcgg gcggtgaagc cgttcggcca gtgcacgatc gttccgttcc gggtgccgcc
     1141 ccagtgcgag gcgacctgct ttgtccactg gtagggcgtg tccatggcgt gcgcccaacc
     1201 gacggcgaag tggttgtagg cctcgggccc tccgaactgg tcgatcttcg aggacatgaa
     1261 ctcggcggtc tcgaggaacg ccgcgccgtt gaacgcgaac gtctcgttga acgtgccgtt
     1321 gatgccgcct tctgccgacg cgccgttgtc gccgatgatg tagtagacga gggtgtcctc
     1381 gagaacgccg agaccgtcga tcgcgtcgat gagccgcccg atgtgatggt cggtatgcgc
     1441 gaggaacgcc gcgtacacct ccatctgacg tgcgaggacg ggcttgagct catcggccat
     1501 gtcgtcccac gccgggatct ctgcgggtcg cgcggtgagc tccgcgtcgg cagggatcac
     1561 cccgagcgcc ttctggcggg cgaagatctc ctcccggagc gcgtcccagc ccgcatcgaa
     1621 gcgaccgcgg tagcgctcga tccagtcctt gggcacgtgg tgaggggcgt gcgtcgcgcc
     1681 gggcgcgaag tacgcgaaga acggccggtc gggcatcaac gacttctgtt gacgcatcca
     1741 tgagatggcc ttgtcggtca tgtcctcggt gaagtggtaa ccctcctccg gagtcttggg
     1801 aggctcgagc ggagtcgtgc cctcatagat cgccgggtag tactggttcg tctcgccgct
     1861 gatgaagccg tagaagtact cgaaaccacc gccaccggtg ggccatgcgt cgaacgggcc
     1921 catcgggctc gactggtgca ccggcacctc gtggcacttg ccgtactgcg aggtggagta
     1981 cccgttcagc ttgaggatgt ccgcgagcgg cgcggccgtg ttggggcgga tcccggtgta
     2041 gcccggcgcc gatgtcgcga gctcggtgat gcaacccatc ccgaccgcat ggtgattgcg
     2101 gccggtgagc aacgcctggc gcgtcggcgc gcacagcgca gtcgtgtgga accgggtgta
     2161 cttcagccct cccgcggcga gccgttccgc gaccggagtg tcgcaaggcc cgccgaacgc
     2221 gctcgaggca gcaaagccga cgtcatcgag gagcacgacg agcacgttcg gcgcacccgc
     2281 gggcggccgc agctcctcgg tcggcgcgaa gctcgtcgcc ggatccttcg cgtcgtacgt
     2341 gaccagaccc ggatatggct gatgcgcatc agggacgggt aggttctgcc ggccgatcat
     2401 cgtttcccgt gccatctgcg ccttctcctt tggaggtcgc gccggcgtgc gcgacacgtg
     2461 ggtagccgtg gcggctcctc acgagccgag agccatggtc ttctgagcgg cgagctcatc
     2521 ctgagaacgc cctggtcctt tggctccgcg agccgcacga gctgggactc ctccagcatc
     2581 attggaaccg cgtgactgcg catcaccacg gggtg
//
LOCUS       DATDBZ010000214         2553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_685044, whole genome shotgun sequence.
ACCESSION   DATDBZ010000214 DATDBZ010000000
VERSION     DATDBZ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685044"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VIX62_05330"
     CDS             complement(<1..1034)
                     /locus_tag="VIX62_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5859686.1"
                     /translation="MKRIKIVGVLLALALVAAACSGGDDEPTDETGSTGATGATGSGP
                     LAEYGESTDADQALVDKAMGPVADVPDIILASIARANQDLDQATLDKAMECWSATKCD
                     TGSGGDLTMGYADGGGLNVWRQVTRMEAILQALTYPEIGKMMFRDAQWSTDPAVAAGD
                     IEFLIQAGVDFIIGYPDQGTNIADAILEAKDAGIPYSTYSAGWVGLPEQEGALIPGED
                     YLTVVGEDLCALGDSFAGVINDGVGSGKVGVMGGTPGNALSLGWQQCYLDETDTSIKN
                     LGPADTYWANDVALQTVNGWLSTDPDIKGYAYEYADGLNTALQAYDDLNVPIDDLTIA
                     LRTDEQTLFCT"
     gene            complement(1113..2168)
                     /locus_tag="VIX62_05335"
     CDS             complement(1113..2168)
                     /locus_tag="VIX62_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859687.1"
                     /translation="MSQPRTTSSSSAVTSFVVASRYMPVYIALLLLVVVAAIWAPATL
                     KGPALSSIAPFGTFLAIAALGQMLVIMTGGIDLSVPGTLTLAAVVTVGLGQTHGDDLG
                     TVILIALALCALVGLVNGLLIGGLGLNALIVTLAVGQIVTGFAIRYYTTTAIQTPVPE
                     ALSEWTSTRFLGVTRIFWMGVAITLVMVVIFRFTTFGRRFQVVGANPTASSIAGLRVN
                     LNQVMAYVVAAILYGIAGILLASFLRTISITIGAPYLLGPIAAVVIGGASLTGGLASP
                     VSTWAAAFFLAGLTQMMRVMGLPTALQFVVFGLVIVGGMLVSGDRIIRGVEHLLRERK
                     RTDLVSAGVIGPGDGTG"
     gene            complement(2165..>2553)
                     /locus_tag="VIX62_05340"
     CDS             complement(2165..>2553)
                     /locus_tag="VIX62_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5859688.1"
                     /translation="FDRKLSGKAGLPRLMTTRDVIRCATSNGARVAGVGDVTGSLEPG
                     RQADVVVLRTDRPNIYPINDPIGAVVWGMDTSNVDMVIAGGRTLMAGGVLSGDVERAR
                     SLAEAARERVAAAAGLTVGAAPGGGG"
BASE COUNT          406 a          906 c          842 g          399 t
ORIGIN      
        1 gtgcagaaca gggtctgctc gtcggtacgc agcgcgatcg tgagatcgtc gatcgggacg
       61 ttcaggtcgt cgtaggcctg caacgcggtg ttgaggccgt cggcgtactc gtaggcgtag
      121 cccttgatgt cgggatcggt cgagagccag ccgttcaccg tctgcagggc cacgtcgttc
      181 gcccagtagg tgtcggccgg acccaggttc ttgatcgatg tgtccgtctc gtccaggtag
      241 cactgctgcc accccagcga cagggcgttg ccgggcgttc cgcccatgac cccgaccttg
      301 ccggaaccca cgccgtcgtt gatgacgccg gcgaagctgt cacccagggc gcacaggtcc
      361 tcgccgacca cggtgaggta gtcctcgccg gggatcagcg caccttcctg ctccgggagt
      421 ccgacccagc cggccgaata cgtggagtag gggatgccgg cgtccttggc ctccaagatc
      481 gcgtcggcga tgttcgtgcc ctggtccggg tagccgatga tgaagtccac gccggcctgg
      541 atcaggaact cgatgtcgcc cgcggcgacc gcggggtccg tgctccactg ggcgtcacgg
      601 aacatcatct tgccgatctc cgggtacgtg agcgcctgga ggatggcctc catgcgggtc
      661 acctgacgcc acacgttgag tccgccgccg tcggcgtagc ccatcgtgag gtcgcccccg
      721 ctgcccgtgt cacacttcgt ggcgctccag cactccatcg ccttgtcgag cgtggcctgg
      781 tcgaggtcct gattcgcccg ggcgatcgag gcgaggatga tgtcgggcac atccgcgacc
      841 ggacccatcg ccttgtcgac gagcgcctgg tcggcgtcgg tgctttcccc gtattccgcg
      901 agcggaccgc tcccggtcgc gccggtggcc cctgtggagc cggtctcatc cgtcggttcg
      961 tcgtccccac cgctacacgc ggctgccacg agggcaagcg cgagaaggac cccgacgatc
     1021 ttgatgcgtt tcatgctcct ccctccttcg atgtcgctgc gcgcaggtga tccgatcggt
     1081 tcggttccac caggtgctgc cccccttctt ccctagcccg tcccgtcgcc gggaccgatg
     1141 acgcccgcgc tcacgagatc cgttctcttg cgttcgcgga gcaggtgctc cacccctcgg
     1201 atgatccggt cacccgaaac caacatgccg cccacgatca cgagcccgaa cacgacgaac
     1261 tgcagggcgg tcggcagtcc catcacccgc atcatctggg tgagtccggc gaggaagaac
     1321 gcggccgccc acgtcgagac cgggctcgcg agtccgccgg tgagcgaggc cccgccgatc
     1381 accaccgccg cgatcgggcc cagcaggtac ggcgcgccga tcgtgatgct gatcgtgcgc
     1441 aggaacgagg ccagcaggat gccggcgatc ccgtacagga tcgccgcgac gacgtaggcc
     1501 atgacctggt tgaggttgac ccgcaggccc gcgatcgagg acgccgtcgg gttcgccccc
     1561 acgacctgga accggcgccc gaacgtcgtg aaccggaaga tcaccaccat cacgagggtg
     1621 atcgcgaccc ccatccagaa gatccgggtc acccccagga acctggtgga cgtccattcc
     1681 gacaatgcct cggggacggg ggtctggatc gcggtggtgg tgtagtaccg gatcgcgaac
     1741 ccggtcacga tctgacccac cgcgagcgtc acgatcaacg cgttcagccc cagcccgccg
     1801 atcaggagcc cgttcacgag gccgacgagg gcgcacagcg cgagggcgat caggatcacc
     1861 gttcccaggt cgtcgccgtg cgtctggccc aatcccaccg tgacgaccgc cgccagcgtc
     1921 agggtgccgg gcacgctgag gtcgatcccg ccggtcatga tcacgagcat ctggccgagc
     1981 gccgcgatgg cgaggaacgt gccgaacggc gcgatcgaac tgagcgccgg ccctttcaac
     2041 gtcgccggcg cccagatcgc ggcgaccacg acgagcagca gcaacgcgat gtacaccggc
     2101 atgtaccgcg aggccacgac gaacgaggta acggccgacg acgaggaggt ggtcctgggc
     2161 tggctcatcc gccgcccccc ggtgccgcgc cgaccgtgag gcccgcggcg gccgccacgc
     2221 gttcccgcgc ggcctcggcc agcgatcggg cgcgctccac gtcaccgctg aggacgccgc
     2281 cggccatcaa ggttcgacca ccggcgatca ccatgtcgac gttcgaggtg tccatgcccc
     2341 agaccaccgc gccgatcggg tcgttgatcg ggtagatgtt cggccggtcg gtgcggagca
     2401 ccacgacatc ggcctgccta cccggctcga gcgaacccgt gacgtcgccc acgccggcca
     2461 cacgcgcgcc gttcgaggtg gcgcaacgga tcacgtcgcg ggtggtcatg agccgcggga
     2521 gcccggcctt gccggagagc ttgcgatcga aca
//
LOCUS       DATDBZ010000215         4033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_891740, whole genome shotgun sequence.
ACCESSION   DATDBZ010000215 DATDBZ010000000
VERSION     DATDBZ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891740"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VIX62_05345"
     CDS             complement(<1..959)
                     /locus_tag="VIX62_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY5859689.1"
                     /translation="MVTKDKNVPSRASTEEPEKTVEDRIELLRKRKKAALTPGGRDSA
                     KKQHDRGKLTARERLDILMDTGSFVETDPFAIHRAHDFGMDRKRPPGDGVVTGYGTVD
                     GRKVFVASQDFTVFGGSMGEVMAQKVCKVMDLAISTGAPFISINDSGGARIQEGAASL
                     AGYGYIFERNVRASGVIPQISVIMGPCAGGAVYSPAITDFTFMVKETSHMFITGPEVI
                     KSVTGEDVSMEELGGAMTHATKSGVASFIGEDDEDVLQRVRYLLSFLPSNNLEDPPAY
                     AATDDPERMAEALTNLIPDRAAEAYDMHEVIRYLVDDGDFLEV"
     gene            complement(994..1458)
                     /locus_tag="VIX62_05350"
     CDS             complement(994..1458)
                     /locus_tag="VIX62_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859690.1"
                     /translation="METEDRRTVGTRSRVAALALVVALTGSLLLVAAPSAQATGPCDF
                     GRGEDQGVRAYMREVIRCAVDRWSVRGGPRTALCIAKRESGLLPWAESSDGINKGLYQ
                     QHVDYWANNYETYARPAWALEKRILNGRTNAIVSIRMAHTGGWGPWGGSDCG"
     gene            complement(1601..2173)
                     /locus_tag="VIX62_05355"
     CDS             complement(1601..2173)
                     /locus_tag="VIX62_05355"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEY5859691.1"
                     /translation="MVSRETDMAETPDDILRALADPERLAIAGALARRDLAARELADA
                     LDLPITRVRKHLNRLTSTGVARLADDRRTYRLDPETLRWAAEQVGPPREAGLALGAAN
                     EDEEIVLRTFFRNGRLTEIPGKAAKRRIVLERIASEFEPGVHYDEKEVNATVGAFFND
                     YASLRRYLVDEGFLDRDAGIYWRAGGRVEV"
     gene            2180..3109
                     /locus_tag="VIX62_05360"
     CDS             2180..3109
                     /locus_tag="VIX62_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY5859692.1"
                     /translation="MQLTDPQQRTLDGLIGTGERPTFSADLPQRLRDRIEEAVRELEL
                     REPLWLGKERLNQHSRCEGQFQAAVSGESSPFAHSSKSAYGVLLHKAIEVEVGARDGM
                     DPHEVAQVAVGRVLDREDRFVEYWSELSASDQDDVLMEVVRRVTLFQASFPPLKPLRR
                     ELAPISELRVRSELLGGDLVLSGQIDLVMGLPDKLEPMRATRLAVDLKTGGAYPEFAE
                     DMRFYALVMTLRFGVPPYRAASLFLESGTWQSEDVTEDLLVHAADRVIGAARAAAGLV
                     NGREAHLRPGTYCTWCPRSSTCPVAELPTAVGA"
     gene            3220..>4033
                     /locus_tag="VIX62_05365"
     CDS             3220..>4033
                     /locus_tag="VIX62_05365"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEY5859693.1"
                     /translation="MGSISRGFRLAKASWGVVRQDHQLLVLPVVSFFCSLVVIAVFAA
                     GAFGIGIPADGESVSPALYVLGFVLYVALAFVTIFFNAAIVGTAMKRLNGEHASIKDG
                     LALARQHLGKIFVWALITATVGMILRTLQDRAGIVGRIVIGLIGVAWNVITFFVVPVL
                     LYEPVGVPEAIKRSASIFRQRWGEQFIGTATIGLAIFLVAIPVVLVGGAVAYAVPLVG
                     VPLLVVAIGALMAVGAACSGVFNAALYRFATTGEASSVFSIEDLNGSFRPKKG"
BASE COUNT          570 a         1381 c         1369 g          713 t
ORIGIN      
        1 aagacctcca ggaagtcgcc gtcgtcgacg aggtagcgga tcacctcatg catgtcgtag
       61 gcctcggcgg cgcgatcggg gatcaggttg gtgagcgctt cggccatgcg ttcggggtcg
      121 tcggtggcgg cgtaggcggg cgggtcctcc aggttgttgg agggcaggaa gctcagcagg
      181 tagcgaaccc gctgcagcac gtcctcgtcg tcctccccga tgaaggacgc gacgccggac
      241 ttcgtggcgt gcgtcatggc gccgccgagc tcctccatgg aaacgtcctc accggtgacc
      301 gacttgatca cctcgggacc ggtgatgaac atgtgcgagg tctccttcac catgaaggtg
      361 aagtcggtga tcgcggggga gtagaccgcc ccgccggcgc agggccccat gatcacgctg
      421 atctgcggga tcacgcccga ggcgcgcacg ttccgctcga agatgtagcc gtagcctgcg
      481 aggctcgccg cgccctcctg gatccgcgcg ccgccggagt cgttgatcga gatgaacggc
      541 gcgccggtgc tgatcgccag gtccatgacc ttgcacacct tctgcgccat gacctcgccc
      601 atcgaaccgc cgaacaccgt gaagtcctgg ctggccacga agaccttgcg gccatcgacc
      661 gtgccgtagc cggtcaccac gccgtcgccg ggcggtcgct tgcggtccat cccgaagtcg
      721 tgcgcccggt ggatcgcgaa cgggtcggtc tccacgaacg agcccgtgtc catcaggatg
      781 tcgagacgtt cacgggcggt gagcttgcct cgatcgtgct gcttcttcgc cgaatcccgg
      841 cccccgggcg tcagggcggc cttcttgcgc tttcggagca gttcgatccg gtcctcgacc
      901 gtcttctccg gctcttcggt gctcgcccgg ctgggaacgt tcttgtcctt ggtcaccatg
      961 tggggagtct agccgcgtgg tccgggctca ggttcagccg cagtcgctgc cgccccaggg
     1021 accccagccg ccggtgtggg ccatgcggat cgacacgatc gcgttcgtgc ggccgttcag
     1081 gatccgcttc tccagcgccc aggcaggcct ggcgtaggtc tcgtagttgt tggcccagta
     1141 gtcgacgtgc tgctggtaga gccccttgtt gatgccgtcg gaggactcgg cccatggcag
     1201 caggccgctc tcccgcttgg cgatgcagag ggcggttcgg ggtcctccgc gcaccgacca
     1261 gcgatcgacg gcgcaccgga tcacctcgcg catgtacgcc cgaacgccct gatcctctcc
     1321 gcggccgaag tcgcacggac cggtggcctg ggcggaagga gcggcgacga gcaggagcga
     1381 gccggtcaac gccacgacca gcgccaatgc cgcgacgcgc gacctggtgc cgacggtcct
     1441 gcggtcctcg gtctccatcc aactccctgg tgttcgtctc acgacgagcg tcaccgatcg
     1501 atgggaagcg ggggatgctg ccggtgacag ccccggcacc ctagcaccgg aggccgcaac
     1561 cgtcgacccg cggtcgaggg tttggcggat cctcagggca tcagacctcg acgcggccgc
     1621 cggcccgcca gtagatcccg gcgtctcggt cgaggaaccc ttcgtccacc aggtaacgac
     1681 ggagcgaggc gtagtcgttg aagaacgccc cgacggtcgc gttgacctcc ttctcgtcgt
     1741 agtgcacgcc gggttcgaac tcggatgcga tccgttccag cacgatgcga cgtttcgcgg
     1801 ccttccccgg gatctcggtg agccggccgt tccggaagaa cgtccgcagc acgatctcct
     1861 cgtcttcgtt ggcggcgccc aatgccaggc cggcttcgcg tggcggtccg acctgctccg
     1921 cggcccaccg gagcgtctcg ggatcaagcc ggtaggtccg gcggtcgtcc gccaggcgcg
     1981 ccacgcccgt cgaggtgagg cggttgagat gcttgcgcac acgggtgatc ggcaggtcga
     2041 gcgcatccgc gagttcccgc gccgccagat cacggcgagc cagcgccccg gcgatcgcga
     2101 ggcgttccgg atcggccagc gcgcgcaaga tgtcgtcagg tgtttcggcc atgtcggtct
     2161 ctctgcttac catgcgcagg tgcaactcac cgacccgcag cagcgcacgc tcgacgggct
     2221 catcggcacg ggggagcgac ccacgttctc cgccgacctc ccacaacgcc tccgcgaccg
     2281 tatcgaggag gctgtccggg agctcgaact ccgcgagccg ctgtggctgg gcaaggagcg
     2341 cctcaaccag cattcgcgct gcgagggaca gttccaggcc gccgtcagcg gtgagtcctc
     2401 accgttcgcg cactcgtcca agagcgccta cggcgtgctc ctgcacaagg ccatcgaggt
     2461 cgaggtcggc gcacgcgacg gcatggatcc ccacgaggtc gcccaggtcg cggtcggccg
     2521 ggtgctcgac cgtgaggatc gcttcgtcga gtactggagc gagctctcgg cgtccgacca
     2581 ggacgacgtg ctcatggagg tggtgcgtcg cgtcacgctc ttccaggcct cgttcccccc
     2641 gttgaagccc ctgcggcgcg agctcgcgcc gatcagtgag ctccgcgtgc ggtccgagct
     2701 gctcggcggc gacctggtgc tgtcgggcca gatcgacctg gtcatgggac tgcccgacaa
     2761 attggagccc atgcgggcca ccaggctggc ggtcgacctg aagaccggcg gggcttaccc
     2821 ggagttcgcc gaggacatgc ggttctacgc gttggtcatg accctgcggt tcggggttcc
     2881 gccctaccgt gccgcgtcgc tgttcctgga gagcggcaca tggcagtccg aagacgtcac
     2941 cgaggatctc ctcgtccacg cggccgatcg ggtgatcgga gccgcccgtg ctgcggccgg
     3001 cctggtcaac ggccgtgaag cccatctgcg gcccggtacc tactgcacgt ggtgcccacg
     3061 ctcgtcgacg tgcccggtcg ccgagctccc caccgccgtc ggcgcctgag aagctccgaa
     3121 ccgccctgca gttccccccg ttcgcgtgcc gatgcaggaa gataggaaca tcgggcgccc
     3181 gcacctcgag ccgcccacga gcgaaaggat cgtcgaacga tggggtcgat ctctcggggc
     3241 ttccgtctcg cgaaggccag ttggggcgtc gtccgccagg accaccagct gttggtgctc
     3301 ccggtcgtct cgttcttctg ctcgttggtc gtgatcgcgg tcttcgcggc tggggccttc
     3361 ggcatcggga tccccgccga cggcgagtcg gtgagccccg cgctctacgt cctcgggttc
     3421 gtgctgtacg tggcgctcgc gttcgtcacg atcttcttca acgccgcgat cgtcgggacc
     3481 gcgatgaagc gcctcaacgg cgagcacgcg tcgatcaagg acgggctggc gctcgcgcga
     3541 cagcacctgg gcaagatctt cgtgtgggcg ctgatcaccg ccaccgtggg gatgatcctc
     3601 cgcaccctgc aggaccgtgc cggcatcgtt gggcggatcg tgatcggcct gatcggcgtc
     3661 gcgtggaacg tgatcacctt cttcgttgtc cccgtcctgc tgtacgagcc cgtcggggtt
     3721 cccgaggcga tcaagcgctc ggcgtcgatc ttccgtcagc gatggggcga gcagttcatc
     3781 ggcaccgcca cgatcgggct cgcgatcttc ctggtggcga tccccgtggt gctcgtcggc
     3841 ggagcggtgg cgtacgccgt gccgctggtg ggtgtgcccc tcctggtggt cgcgatcggt
     3901 gcgctcatgg ccgtcggggc cgcatgctcc ggcgtgttca acgccgccct gtatcggttc
     3961 gccacgacgg gtgaggcatc cagcgtgttc tcgatcgagg atctcaacgg ctccttccgg
     4021 ccgaagaagg ggt
//
LOCUS       DATDBZ010000216         6434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2927826, whole genome shotgun sequence.
ACCESSION   DATDBZ010000216 DATDBZ010000000
VERSION     DATDBZ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2927826"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VIX62_05370"
     CDS             complement(<1..452)
                     /locus_tag="VIX62_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY5859694.1"
                     /translation="MKDVGLVGVPFSGKSTLFTAMTRAGSQGGQANIAVVPVPDPRLQ
                     VLTDMETSKKTVAAQVRFVDVPGGVSSAQGIAKLREVDALAVVLRCFGADASPADELE
                     TVRGELLLADLGVIDSALEKAEKKARGKPGPEVDALRRAKEALEAETP"
     gene            complement(795..1187)
                     /gene="sodN"
                     /locus_tag="VIX62_05375"
     CDS             complement(795..1187)
                     /gene="sodN"
                     /locus_tag="VIX62_05375"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEY5859695.1"
                     /translation="MFEAFLKPRRTVHAHCDLPCGVYDPAQARIEAESVKACQEKYQG
                     SDDAIFQERAITIKEERADLVKHHLWVLWTDYFKPEHVEKYPDLHDKFWKATKLAGDS
                     KKSTDPAQGQQLLDAIDELGKIFWETKS"
     gene            1186..1662
                     /locus_tag="VIX62_05380"
     CDS             1186..1662
                     /locus_tag="VIX62_05380"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEY5859696.1"
                     /translation="MEPPPSFDDRSLGLEPETYHRGWKRASGAVAGAVVVAGVAWVCL
                     RWKPSRIEIDGGSMAPTLAPGDWALVATPASYARGDVVMVEHPGRPGFEMVKRILGVP
                     GDVRDGRTLGADEWWVEGDFEPASTDSRQFGPVTTEELKAKVLVIYWPKERRARVR"
     gene            1841..2542
                     /locus_tag="VIX62_05385"
     CDS             1841..2542
                     /locus_tag="VIX62_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859697.1"
                     /translation="MVGSAVLTIVFLVAGIFASVLFRTPTPQGSADEYRFLARDFSGD
                     PIRWNPCDPIHYVTNLTDAPAGSIDDVTESVRRVSMATGIQFVDDGETDEEPSQRRDS
                     YQPDRYGDEWAPVLIAWVDPDDSLISFSSDGHTAAGVAAPQVPSDGSDVLVSGWVAIN
                     ADDPNGPGFGWMGAQGPVVLHELGHVMGLDHVKVDGELMEGAGGGVTDFGPGDLEGLR
                     ILGRSSGCLNTPDPG"
     gene            2834..3079
                     /locus_tag="VIX62_05390"
     CDS             2834..3079
                     /locus_tag="VIX62_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY5859698.1"
                     /translation="MSVVPSTATVTVDARGQSCPGPLVSLAKALRDAASGDLLELLAT
                     DPGSKSDVPSWAEISGNELVGSDEPTPGQYRYLVRKV"
     gene            3086..3655
                     /locus_tag="VIX62_05395"
     CDS             3086..3655
                     /locus_tag="VIX62_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY5859699.1"
                     /translation="MTAETVATDAPTYAELLARIEALEAGQSPVKKKNKLTIIAWGGD
                     LDRIWPTTILATTAAASGMESSVFFTFWGLFAIVKPGVRITGDNWMQKMMSAMNPGSA
                     QKAKLSRYHFAGAGPTMFKKLADDHQVAKPEELIATAQDLGVRLIPCQMTMDLLGLKH
                     EDLLEGLEEPIGAATAIAEMRDAAVSLFI"
     gene            3675..5045
                     /gene="chrA"
                     /locus_tag="VIX62_05400"
     CDS             3675..5045
                     /gene="chrA"
                     /locus_tag="VIX62_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY5859700.1"
                     /translation="MSDETPQHGVTFREAARFWVKLGFINFGGPAGQIAIMHEEIVQR
                     RRWVSNGRFLHALNFCMLLPGPEAQQLAIYLGWLLHKVRGGLVAGVMFVLPSFFVLLA
                     LSWVYVAHGDVTWVSATLAGLAPAVIAIVASATIRIGQKALANGLMVAVAVAAFVAIY
                     VVHVPFPVIVLVAGAIGLVGGSAWPDRFRVGVVEELEEHERVAIADDAPRAEHTKPTV
                     TRTLVTLAVGLVVWLVPIALVAAWRGPGDTLTQMGWFFSRASLVTFGGAYAVLAYVNV
                     AAVSTYGWLLPGQMVVGLGLAETTPGPLIMVLEFVGFVGAYQHPGDLDPLVAGVLGAC
                     VVVWATFAPCFLWIFLGAPYIEGLRGNRRLGAALETITAAVVGVIANLAVTFALSTLF
                     EEVRVSRVLGRFDVVRPVWSSVDPFAVVVALVAFVGMWRFRWKVIPVVAGSALAGLLW
                     YGLLGN"
     gene            complement(5052..5783)
                     /locus_tag="VIX62_05405"
     CDS             complement(5052..5783)
                     /locus_tag="VIX62_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015034688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5859701.1"
                     /translation="MPRDWDAATYDRLPIPMTAWGGTVLGWLDLRGDERVLDAGCGTG
                     KVTEMLRDRLPRGSVVALDGSVSMVEQARARLGDDRVEYLVADLREPLPVSPPVDAIL
                     STATFHWIADHDALFANLAAVLRSGGQLAAQCGGAGNIASIEAALGQIDEDLRGRKHF
                     ATPEATAARLGAAGFVEVETWLHEEPTPLPEGDLEPYLETICLGDHVEGMSPEQRRAF
                     VHDVASRMPGPLIDYVRLNIRARRA"
     gene            complement(5837..>6434)
                     /locus_tag="VIX62_05410"
     CDS             complement(5837..>6434)
                     /locus_tag="VIX62_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859702.1"
                     /translation="KGRRSMIITAVIALVLVALGGTYLLAGGDDDDAASPSATVSRSP
                     EPSPTEEPSVSPTESPTASASPSLSADPNILDNGEHFVFMNGAKTGAARRLTFDLAEW
                     YTDQEAIDVAAQHGDDAPNGYYIVNDNPRLRTMPVSDDVVVRYIPNDACCEPQPGNFD
                     ALAEAVNGTAQTDYFAQAPWWITVSGGQIVEIEQQYLP"
BASE COUNT          905 a         2177 c         2226 g         1126 t
ORIGIN      
        1 aacggcgtct cggcctcgag cgcttccttc gctcggcgca acgcgtccac ctcaggaccg
       61 ggttttccac gtgccttctt ctcggccttc tccagggcgc tgtcgatcac gccgaggtcg
      121 gcgagtagca actctccgcg cacggtctcg agttcgtcgg caggcgaagc gtccgcgccg
      181 aagcagcgca acaccaccgc gagtgcgtcc acctcgcgca gcttcgcgat gccctgggcg
      241 ctcgacacac cgccgggcac gtccacgaac cggacctgcg ccgcgacggt cttcttcgac
      301 gtctccatat cggtgagcac ctgtaggcgc ggatcgggca ccggcacgac tgcgatgttg
      361 gcctggccgc cctgcgaacc cgcgcgcgtc atcgcggtga acagcgtgct cttaccgctg
      421 aacggcaccc cgacgagtcc gacgtccttc acggtcggcg agcctaacag ggcgtcccct
      481 gcggacggcc cgagccgttc gctcccggtg gctccgccgt tcggcccaca gctctggccc
      541 ttcgaccact gtgccgccgg cgtggccgat gccgatgtat cgctacgaga ccgggcgggg
      601 tggggtgccg ccagacccac tccacctcgc caggtccgtc tctacgtccc ggtggatgtg
      661 ggatccgacg gcctcgtcca ccgggtcact ttccccgtac gaggggggat gggtacgggt
      721 ctggtgacga cgaagcccgg ggcacctggc gccccgggct tcgttacgag cggtcgtgca
      781 gggatcgacc tagatcagga cttggtctcc cagaagatct tgccgagctc gtcgatcgcg
      841 tcgaggagct gctggccctg cgccgggtcc gtggacttct tggagtcgcc ggcgagcttc
      901 gtcgccttcc agaacttgtc gtgcaggtcc gggtacttct ccacgtgctc gggcttgaag
      961 tagtcggtcc acagcaccca caggtggtgc ttgaccaggt cggcccgctc ttccttgatc
     1021 gtgatcgcgc gctcctggaa gatcgcgtcg tcgctgccct gatacttctc ctggcaggcc
     1081 ttcacggact ccgcttcgat gcgtgcttgc gcggggtcgt acacaccaca cggcaggtcg
     1141 caatgcgcat ggacggtgcg gcgtggcttg aggaatgctt cgaacatgga accgcctcct
     1201 tcgttcgatg accgttccct cggcctcgaa cccgaaacct accaccgcgg ttggaaacgg
     1261 gcgtctggag ccgtcgccgg cgccgtcgtg gtggccggtg tcgcgtgggt atgtctgcgg
     1321 tggaagccct cgcgcatcga gatcgatggc ggctccatgg cccccaccct ggcccccggc
     1381 gattgggcgc tggtcgcgac gcccgcgtcg tacgcacgcg gcgacgtcgt catggtcgaa
     1441 caccccgggc gtcccggctt cgagatggtg aagcgcatcc tgggcgttcc cggagacgtc
     1501 cgagacggtc ggacgctcgg cgcggacgaa tggtgggtcg agggcgactt cgaacccgcc
     1561 tctaccgaca gccgtcagtt cggccccgtc acgacggaag aactgaaggc gaaggtcctc
     1621 gtgatctact ggccgaagga acgccgagcc agggttcggt gaaccatcgg aactgccgat
     1681 gagccctacg atgattccgt ctgcgtccta ggagctcgtc acgtcccagt accttccctc
     1741 acagatcccg cagcgacccg atcagacgcc ttcgggcgtt ccgctgccgc cgtggccgcc
     1801 gctcgttccg gggccgcacc gacgccgata cccagcgtgg gtggtcgggt ctgcggtgct
     1861 cacgatcgtg ttcctggtgg cgggcatctt cgcgtccgtg ctcttccgga cccccacgcc
     1921 gcagggcagc gccgacgaat atcgcttcct ggctcgcgac ttctccggtg atccgatccg
     1981 gtggaacccg tgcgatccga tccactacgt gaccaacctc accgatgcac cggcgggttc
     2041 gatcgacgac gtcaccgagt cggtccgtcg cgtgtccatg gccaccggga tccagttcgt
     2101 cgacgacggc gagaccgacg aggaacccag ccagcgtcgt gactcctatc aacctgatcg
     2161 gtacggcgac gagtgggcgc ctgtgctgat cgcttgggtc gatccggacg attccctgat
     2221 ctcgttctcc tccgacggcc acacggcggc cggggtggcg gcgccgcagg tgccttcgga
     2281 cggcagcgac gtcctggtga gcggctgggt cgcgatcaac gccgacgatc ccaacgggcc
     2341 cggcttcgga tggatgggcg cgcaggggcc cgtggtgctg cacgaactcg gccacgtgat
     2401 gggcctggac cacgtgaagg tcgacggcga gctgatggag ggcgccggag gcggcgtcac
     2461 cgacttcggt ccgggtgacc tggaagggct gcgcatcctg ggtcgttcgt cagggtgctt
     2521 gaacacgccc gacccgggct agccctcgtc tccagcaacc ggcggtcgtg ggtggcgatc
     2581 acgaacgtcg tcaccgtgtc ggccggtcta ccgagtgcga gtggctaggc cggaaggcgc
     2641 ttgttcggaa tggtcggggt cgggtaggct gttgcacctg caaatgatct gcacacgcga
     2701 agccggcatc gtgggggcca tgatccccga tcgcatctgc tgttccgcgt gacccggagc
     2761 ggtcccaacg ctccccgctg atcctgccac tccctttccg caggctcctc accgctccgt
     2821 ctggaggtcc tccatgtccg tcgttcccag caccgcaacc gtcaccgtcg acgcacgcgg
     2881 gcagtcgtgc cccggcccgt tggtgtccct ggccaaggcc ctgagggacg ccgcatcggg
     2941 tgacctgctc gagctcctgg cgaccgaccc gggctccaag tccgacgtcc ccagctgggc
     3001 cgagatcagc ggcaacgagt tggtcggatc cgacgaaccc acgccgggtc agtaccgcta
     3061 cctggtccgc aaggtgtgat cttcgatgac tgccgaaacc gtcgccaccg acgctcccac
     3121 ctacgccgag ctcctcgccc ggatcgaggc gctcgaggcc ggccagagcc cggtgaagaa
     3181 gaagaacaag ctgacgatca tcgcctgggg cggtgacctc gaccgcatct ggcccaccac
     3241 gatcctggcc accaccgccg cagcgagcgg catggagtcc agcgtgttct tcaccttctg
     3301 ggggctgttc gccatcgtga agcccggcgt gcgcatcacc ggggacaact ggatgcagaa
     3361 gatgatgagc gccatgaacc cgggttcggc gcagaaggcc aagctctcgc gctaccactt
     3421 cgccggcgcc ggcccgacca tgttcaagaa gctcgcggac gaccaccagg tggccaagcc
     3481 cgaggagttg atcgccaccg cccaggatct cggggttcgg ttgatcccgt gccagatgac
     3541 catggacctg ctggggctca agcacgagga cctgctcgaa ggactcgagg agccgatcgg
     3601 cgccgccacc gcgatcgccg agatgcgcga cgccgcggta tcgctcttca tctgacccac
     3661 ctacactccg ccccgtgagc gacgagacgc cgcagcacgg cgtgacgttc cgtgaagccg
     3721 cgcgcttctg ggtgaagctc gggttcatca acttcggcgg ccccgccggc cagatcgcga
     3781 tcatgcacga ggagatcgtg caacgccgtc gctgggtgtc gaacggccgg ttcctgcatg
     3841 ccctgaactt ctgcatgctg ctgcccggtc ccgaggccca gcagctcgcg atctacctcg
     3901 gctggctcct gcacaaggtg cgcggcggtc tcgtggccgg cgtgatgttc gtcctgccga
     3961 gcttcttcgt gttgctggcg ctgtcgtggg tgtacgtggc gcacggcgac gtcacctggg
     4021 tgtcggcgac gctggcggga ctggcgccgg cggtgatcgc gatcgtggcc tcggcgacga
     4081 tccgcatcgg ccagaaagcg ctcgcgaacg gtctcatggt ggccgttgcc gtggcggcgt
     4141 tcgtggcgat ctacgtggtg cacgtgccgt tcccggtgat cgtgctggtc gccggcgcga
     4201 tcgggctcgt gggcggctcg gcgtggcccg accggttccg cgtcggggtg gtcgaggaac
     4261 tggaggagca cgagcgggtc gcgatcgcgg acgatgctcc gcgggccgag cacacgaagc
     4321 cgaccgtgac ccggacgctg gtgacgctcg cggtggggct ggtggtgtgg ttggtaccga
     4381 tcgcgttggt cgctgcctgg cgcgggcccg gcgacacgct cacgcagatg gggtggttct
     4441 tcagccgggc atcgctggtg acgttcggcg gggcgtacgc ggtgctcgcc tacgtgaacg
     4501 tggcggcggt ctcgacgtac ggatggctgc tcccgggcca gatggtagtg gggctcgggc
     4561 tggccgagac cacgccgggg ccgctgatca tggtgctgga gttcgtgggg ttcgtcggcg
     4621 catatcagca tccgggcgac ctggacccgc tggtggcggg cgtgctgggg gcgtgcgtgg
     4681 tggtgtgggc gacgttcgcg ccgtgcttcc tgtggatctt cctgggcgcg ccctatatcg
     4741 aggggctacg cgggaaccgg cggctcggcg cggctctcga gacgatcacg gcggcggtgg
     4801 tgggcgtgat cgcgaacctg gcggtgacgt tcgcgctgtc gaccctgttc gaggaggtac
     4861 gggtgtcgcg cgtgctcggg cggttcgatg tggtgcggcc ggtgtggtcg agcgtggacc
     4921 cgttcgcggt cgtggtggcc ctggtggcct tcgtggggat gtggcggttc cgatggaagg
     4981 tgatccccgt ggtggcggga tcggcgctgg ccgggttgct ctggtacggc ctgctcggga
     5041 actgaggggg ttcaggctcg gcgggcgcgg atgttcagcc gcacgtagtc gatcagcggc
     5101 ccgggcatcc tcgacgccac gtcgtgaacg aacgcccgac gctgctccgg gctcatcccc
     5161 tccacatgat cgcccaggca gatcgtctcc aggtacggct ccagatcacc ctcgggcagc
     5221 ggcgttggct cctcgtgcag ccacgtttcc acttcgacga acccggccgc tcccagccgg
     5281 gcggccgtgg cctccggcgt ggcgaagtgc ttccgtccgc gaaggtcctc gtcgatctgg
     5341 cccaacgccg cttcgatcga ggcgatgttg ccggcgcctc cgcattgggc cgccagctgt
     5401 ccgcccgacc gcaggacggc cgccaggttc gcgaacagcg cgtcgtggtc ggcgatccag
     5461 tggaaggtgg ccgtcgacag gatcgcgtcc accggaggcg acaccggcaa cggttcccgc
     5521 aggtcggcca ccaggtactc cacccggtcg tcgccgagcc gggcgcgcgc ctgctccacc
     5581 atcgagaccg aaccgtccag cgccaccacc gagccgcgag ggagccggtc acgcagcatc
     5641 tccgtgacct tgccggttcc gcagccggca tccagcacgc gctcgtcacc ccggaggtcc
     5701 agccagccga ggacggtccc gccccacgcc gtcatgggga tgggcaggcg gtcgtacgtg
     5761 gcggcgtccc aatctcgcgg catccccggc aggctaccgg gagtttccgg cgcgcccctg
     5821 gccgaccgtg gaaccctcag ggcagatact gctgctcgat ctccacgatc tggccgccgc
     5881 tcacagtgat ccaccacggc gcctgcgcga agtagtcggt ttgcgccgtg ccgttcaccg
     5941 cctccgccaa ggcgtcgaag ttgccgggct ggggctcgca gcaggcatcg ttggggatgt
     6001 atcgcaccac cacgtcgtcc gataccggca tcgtgcggag ccgagggttg tcgttgacga
     6061 tgtagtaacc gttcggcgcg tcgtcgccgt gctgtgccgc aacgtcgatc gcctcctggt
     6121 ccgtgtacca ctcggcgagg tcgaaggtga ggcgccgtgc cgcccccgtt ttcgctccgt
     6181 tcatgaacac gaagtgctct ccgttgtcca ggatgttcgg atcggcggag agggacggcg
     6241 atgcggaggc cgtgggggac tccgtgggcg agacggaggg ttcctcggtg gggctgggct
     6301 cgggcgatcg gctgaccgtc gccgatgggg aggcggcgtc gtcgtcgtca cctccggcca
     6361 acaggtaggt gcctcccagg gccaccagca ccagcgcgat cacggcggtg atgatcatgc
     6421 tccgccggcc cttc
//
LOCUS       DATDBZ010000217         2853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1912559, whole genome shotgun sequence.
ACCESSION   DATDBZ010000217 DATDBZ010000000
VERSION     DATDBZ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912559"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..606
                     /locus_tag="VIX62_05415"
     CDS             151..606
                     /locus_tag="VIX62_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015578346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY5859703.1"
                     /translation="MKTMLRPAVTVQYPHVKPDLPARTRGVIALKQENCTVCYKCSRE
                     CPDWCIYIDAHKETHEPAGGGRGRSMKVLDRFAIDYALCMYCGICVEVCPFDALFWSP
                     EFEYAEYEMDLLIHEKERLEEWTYTVLPPPALEVGAEPSADDLAAEESA"
     gene            603..1103
                     /locus_tag="VIX62_05420"
     CDS             603..1103
                     /locus_tag="VIX62_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY5859704.1"
                     /translation="MSAQEYAFCVLAVVGGLSAIAVVTVRNVVHAALYLVVALLSVAG
                     VYLLLGAEFVAWVQILIYVGAIVILFLFGLMLTKAPIGRDSLDNQQRWLGALVGVGVF
                     AGLVFLIQQAYPVQDAVAFQTVAGTTDMVGEAIFRNYVLPFEAVSFLLLAALIGAIVL
                     ARKDDA"
     gene            1100..1402
                     /gene="nuoK"
                     /locus_tag="VIX62_05425"
     CDS             1100..1402
                     /gene="nuoK"
                     /locus_tag="VIX62_05425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY5859705.1"
                     /translation="MIRLPLVLMFSALLFSAGVYGVLARRNAVMVLMAIELMLNAVNV
                     NLVAFAASMQEMTGQVFALFVIAVAAAEVGIGLAIVILLFRTRESINVDEVNLLKW"
     gene            1399..>2853
                     /gene="nuoL"
                     /locus_tag="VIX62_05430"
     CDS             1399..>2853
                     /gene="nuoL"
                     /locus_tag="VIX62_05430"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY5859706.1"
                     /translation="MTALASEGEAAFKATTDGIWFVEHAWIVVLLPLISAALILFFGK
                     RTPGKGAVVGIAAVAAAFAFSLGVLWHFVEGGGPFEAGIEWFTVGSLHLELGILVDGL
                     TGIMLVVVTAVSLCVHVYSLGYMQGDERFTWFYVVLSLFTAAMLTVVVSNNLIQLLVG
                     WEVMGVCSYLLIGHWWEVKENSNAAIKAFITTRVGDIPFMFGIIMLIALTGFETTNIT
                     VISHELGLGVASPLFISMAAILLFGGTVGKSAQFPLHVWLPDAMAGPTPVSALIHAAT
                     MVAAGVYLIGRLFEVFLQAEPWVLTTVSVVAAITALGAALLAIVQDDIKRVLAYSTLS
                     QLAYMVAGLSLGELGLTAGFFHLFTHAFFKALLFLGAGSVIHAVHSNNMSEMGGLRKS
                     MPVTFWTMLIGSLALAGIFPLAGFWSKDELLVVAQEEHVTWLFVVFLITAVITAFYTI
                     RMVLLTFFGEYKGHAHPHESPRTMTGPLVALAAAT"
BASE COUNT          397 a          952 c          948 g          556 t
ORIGIN      
        1 atctcgctcg tatggatcct gggcgctgcc gtcttcaagg tcgccttcta accgacaagg
       61 acgaacgaca ccgtggccat cgaccgaacg cacatcgacc agcccgagtc gggggtcggc
      121 cagggcctgc tcaagggcct cgcggtcacg atgaagacga tgctgcgccc ggcggtcacg
      181 gtgcagtacc cgcacgtgaa gcccgacctg ccggcgcgga cccgcggtgt catcgccctg
      241 aagcaggaga actgcaccgt ctgctacaag tgctcgcgcg agtgccccga ctggtgcatc
      301 tacatcgacg cccacaagga gacccacgag ccggcgggcg gcggccgcgg ccgttcgatg
      361 aaggtgctcg accgcttcgc gatcgactat gcgctgtgca tgtactgcgg catctgcgtc
      421 gaggtctgcc cgttcgatgc cctcttctgg agccccgagt tcgagtacgc ggaatacgag
      481 atggacctgc tgatccacga gaaggaacga ctcgaggagt ggacctacac cgtgctgccg
      541 ccacccgcgc tggaggtcgg cgccgagccg agcgccgacg acctcgcggc cgaggagagc
      601 gcatgagtgc acaggagtac gcgttctgcg tgctcgccgt ggtcggcggc ctgtccgcca
      661 tcgccgtggt gaccgtgcgc aacgtcgtgc acgcggcgct gtacctcgtg gtcgcgctgc
      721 tctcggtcgc cggggtgtac ctgctgctgg gcgcggagtt cgtggcatgg gtgcagatcc
      781 tgatctacgt gggtgcgatc gtgatcttgt tcctgttcgg cctgatgctc acgaaggccc
      841 ccatcgggcg cgactcgctc gacaaccagc agcgctggct cggcgccctg gtcggcgtcg
      901 gcgtgttcgc cgggctcgtg ttcctgatcc agcaggccta tccggtgcag gacgccgtcg
      961 cgttccagac ggtcgcgggc accaccgaca tggtcggcga ggcgatcttc cgcaactacg
     1021 tgttgccgtt cgaggccgtg tcgttcctgc tgctcgcggc cctgatcggc gccatcgtgc
     1081 tggcccgcaa ggacgacgca tgatccggct gcccctggtg ctgatgttct cggccctgct
     1141 gttctcggcg ggggtctacg gggtgctggc ccgccgcaac gcggtgatgg tgctcatggc
     1201 gatcgagctg atgttgaacg ccgtcaacgt gaacctggtc gcgttcgcgg catcgatgca
     1261 ggagatgacg gggcaggtct tcgcgttgtt cgtgatcgcg gtggcggccg ccgaagtggg
     1321 catcggtctc gcgatcgtga tcttgctctt ccgcacacgc gagtcgatca acgtggacga
     1381 ggtgaacctg ctcaagtggt gaccgcgctg gcgtccgagg gggaggcggc cttcaaggcc
     1441 acgaccgacg gcatctggtt cgtcgagcac gcctggatcg tggtgctgct gccgctgatc
     1501 tcggcggcgc tcatcctgtt cttcggcaag cgcacgcccg gcaagggcgc ggtcgtcggt
     1561 atcgccgcgg tggcggccgc gttcgcgttc tcgctcggcg tgctctggca cttcgtggaa
     1621 ggcggggggc cgttcgaagc cggcatcgag tggttcacgg tgggctcgtt gcacctggag
     1681 ctcggcatcc tcgtcgacgg actcaccggc atcatgctcg tcgtcgtcac ggcggtctcg
     1741 ctctgcgtgc acgtctactc gctcggctac atgcagggcg acgagcgctt cacctggttc
     1801 tacgtggtgc tgtcgctgtt caccgccgcg atgctcacgg tcgtcgtctc gaacaacctg
     1861 atccagctgc tcgtgggctg ggaggtcatg ggcgtgtgct cctacctctt gatcggccac
     1921 tggtgggagg tcaaggagaa ctcgaacgcc gcgatcaagg cgttcatcac cacacgcgtc
     1981 ggcgacatcc cgttcatgtt cggcatcatc atgctgatcg ccctcaccgg gttcgagacg
     2041 acgaacatca ccgtgatctc ccacgagctc ggactcggtg tcgcttcgcc gctgttcatc
     2101 tcgatggcgg ccatcctgct gttcgggggc accgtcggga agtcggccca gttcccgctg
     2161 cacgtatggc tgcccgacgc gatggcgggc cccacgccgg tgtcggccct gatccatgcc
     2221 gccacgatgg tcgccgcggg cgtctacctc atcggccgtc tcttcgaggt gttcctgcag
     2281 gccgagccgt gggtgctcac cacggtcagc gtcgtggcgg cgatcaccgc cctcggcgcc
     2341 gccctgctcg cgatcgtgca ggacgacatc aagcgtgtgc tcgcgtactc gaccctctcg
     2401 cagctcgcct acatggtcgc gggtctctcg ctcggtgagc tcgggctcac cgccgggttc
     2461 ttccacctat tcacgcacgc gttcttcaag gcgttgctct tcctcggcgc gggttcggtg
     2521 atccacgccg tgcactccaa caacatgagc gagatgggcg gcctgcggaa gtcgatgccg
     2581 gtcaccttct ggacgatgct gatcggctcg ctcgcgctcg ccggcatctt cccgctcgcg
     2641 gggttctggt cgaaggacga gctgctcgtg gtcgcgcagg aagagcacgt gacgtggctc
     2701 ttcgtcgtct tcctgatcac cgccgtgatc accgccttct acacgatccg catggtgctg
     2761 ctcaccttct tcggggagta caaggggcac gcgcacccgc acgagtcgcc gcgcacgatg
     2821 accggcccgc tcgtcgcgct cgccgccgcc acg
//
LOCUS       DATDBZ010000218         4108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2118787, whole genome shotgun sequence.
ACCESSION   DATDBZ010000218 DATDBZ010000000
VERSION     DATDBZ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2118787"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VIX62_05435"
     CDS             complement(<1..464)
                     /locus_tag="VIX62_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY5859707.1"
                     /translation="MDTQRLLVLGRDELESLHLSWTEVVDVLEDAFVQKARGLVQNPP
                     KPAVRPRSDAFINAMPAYLGGSDRVGIKWVAGFEQNRAKGLPYIYGVFVLNDADTGRP
                     LALMDGGWITEMHTAGVSGVAVRQVAAQPHRLAIVGAGVQGRRHLELLLDRCP"
     gene            complement(485..2590)
                     /locus_tag="VIX62_05440"
     CDS             complement(485..2590)
                     /locus_tag="VIX62_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY5859708.1"
                     /translation="MNGDTRTALERMLEARSVAVVGASVKPLSLGNQMVVELQRGGYE
                     GSIYPVNPGYEEVLGLRCYPSIAELPEPVDLAILGVSNARVEQALRDAVAAGARSAVT
                     FSSLFEEEPPADVPALPERLGAIARSAGIAMCGGNGMGFLNLDTGLRATGFATPDDMR
                     AGPVTFISHSGSAFAAVAFNDRGIGFNLIVSSGQEIVSTMADYMDYALGLPSTRVLAL
                     LLETVRDPQRFCAQLRRAAEADIPVVALTVGRTEGSKAMVTAHSGALAGEHGAYEAVF
                     DAYGVHEVLTLDEMADAMELFSSPRRVRGTARGIASVHDSGGERALFVDVATDLGVPF
                     AAITEQTRSAIEDTLDPGLDATNPLDAWGTGIDADRIFRESFLVMHEDPDAAALAFVV
                     DLTRQGEPYDEGYLQVAKDVFAATTKPFCIVSNLASAVALEEAAYLRDAGIPVLEGTR
                     SGLVALRALIAQRDAGDRPPLEAPTPVAEDVRDRWRQRLGEGSEVDELEGLRLLADYG
                     IPVIPTREAGSVAVAVGAANELGWPVAIKTAAPGVQHKSDVGGVALGLADAEELRTAY
                     EDIAARLGPRVVVAPMAPKGVEVALGIVRDPTFGPLVLVAAGGVLVELMKDRQLALPP
                     LDEHRARSLIDRLRTRPLLDGVRGAPAADVASLAFAISRLSVLASDLGDLIAALDVNP
                     MIVSPGGCVAVDALVIPVS"
     gene            complement(2619..>4108)
                     /locus_tag="VIX62_05445"
     CDS             complement(2619..>4108)
                     /locus_tag="VIX62_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5859709.1"
                     /translation="DTSAPFTDHPDINVTTYSYVMSLMPPTIIRELNLKRHGYDVTPF
                     GPYFQAFPDGRAITVYADDSQKSYDSIAQFSKKDADTLPKWEAWMKGVSDVLGPLLLS
                     VPPHVGSTKLGDLLPQAQTAWKMRGLGQRGVADVTRLFTMSVTDLLNDWFESDAIKAM
                     LTVNGVIGTWAGPDEPGTAYVMLHHSIGDVGDGHLGSWGFQQGGMGAVSDSIRRSAES
                     FGAEIRTDAKVAKILTRGGRAVGVALENGEELRAPVVVTTIHPKIAFLDLLDKHELPA
                     DFVWDIERWKTRSGTVKVNVAISELPNFTAIPGTNQQDHHTGSVELCFSPQYAERAFQ
                     DAHIERRPATAPFVDGTIPTTLDKQLAPEGTHVFSMFTQWVPDDWNTEPHRDELDAYA
                     QRIFDGYNDLAPNFKDSIIDYQVIGPYDMEQDLGLIGGNIFHGELSVDQLFHMRPAPG
                     YADFRTPIRGLYHGSCATHGGGGVNGIPGWQAYKMAVKDKAVAKK"
BASE COUNT          642 a         1447 c         1389 g          630 t
ORIGIN      
        1 gggcatcggt ccagcaacag ctcaagatgc cgccgtccct ggaccccggc cccgacgatc
       61 gccagccggt ggggctgtgc agccacctgt cgaaccgcga caccggagac gccggcggtg
      121 tgcatctcgg tgatccaacc gccgtccatc aaggcgagcg ggcgaccggt gtcggcatcg
      181 ttcagcacga acacgccgta gatgtacggc aaccctttcg cgcggttctg ctcgaagccg
      241 gccacccatt tgatcccgac gcgatcgctg ccgcccaggt aggcgggcat ggcgttgatg
      301 aaggcgtcgc tgcgaggacg cacggccggc ttggggggat tctggacgag gccccgtgcc
      361 ttctggacga acgcgtcctc aagcacatcg accacctcgg tccaggagag atggagcgac
      421 tccagctcgt cgcgaccgag gaccagcaac cgctgcgtgt ccacgaccgg agtctattgc
      481 gcctctatga gacggggatc acgagggcgt cgacggcgac gcacccgccg ggcgagacga
      541 tcatcgggtt cacgtcgagc gcggcgatca ggtcgccgag gtcgctcgcc aggacggaga
      601 gccgtgagat cgcgaacgcc agcgatgcga cgtcggcagc gggcgcacca cgaaccccgt
      661 ccagcaacgg gcgagtccgc aaccgatcga tgagcgagcg cgcccgatgt tcgtcgagcg
      721 ggggcagcgc gagctgccgg tccttcatca gctcgaccag gaccccgccc gccgccacca
      781 gcacgagcgg cccgaacgtg gggtcgcgca cgatgccgag cgccacctcg acgcccttcg
      841 gcgccatggg ggcgaccacc acccggggtc cgagccgcgc ggcgatgtcc tcgtacgcgg
      901 tacggagctc ctcggcgtcg gcgagtccca gcgcgacccc gccaacatcc gacttgtgct
      961 gcacgcccgg cgcggcagtc ttgatcgcga ccggccaccc gagttcgttg gcggcgccca
     1021 ccgccacggc caccgacccg gcctcccggg tggggatcac ggggatgccg tagtcggcca
     1081 gcaggcgcag accttcgagc tcgtcgacct ccgagccttc gccgaggcgt tgccgccacc
     1141 ggtcgcgaac gtcctcggcc accggcgtgg gtgcttccag cggcgggcgg tcaccggcat
     1201 cgcgctgggc gatcaacgcc cgcaacgcca ccaaccccga ccgcgtgcct tcgagcacgg
     1261 ggatgcccgc atcgcgcagg taggcggcct cctccagcgc caccgcgctc gccaggttcg
     1321 acacgatgca gaagggcttc gtcgttgccg cgaacacgtc cttggccacc tgcaggtagc
     1381 cctcgtcgta cggctcgccc tgccgcgtga ggtcgaccac gaacgccagc gccgcggcgt
     1441 cggggtcctc gtgcatcacg aggaacgact cgcggaagat ccggtcggcg tcgatcccgg
     1501 tgccccaggc atcgagcggg ttggtcgcat ccagccccgg gtccagcgtg tcctcgatcg
     1561 ccgagcgcgt ctgctcggtg atggcggcga acggcacgcc gaggtcggtg gccacatcca
     1621 cgaacaaggc gcgctccccg cctgaatcgt gcacggaggc gatgccccgg gccgtgccgc
     1681 gcacccggcg tgggctggag aacagttcca tcgcgtcggc catctcgtcg agcgtcagca
     1741 cctcgtgcac gccgtacgcg tcgaacaccg cttcgtaggc gccgtgctcg ccggccagcg
     1801 cgccggagtg ggcggtcacc atcgccttcg acccctcggt gcgccccacc gtcaacgcca
     1861 ccaccgggat gtcggcctcg gcggctcgcc ggagctgggc gcaaaaccgc tgcggatcgc
     1921 gcacggtctc gagcagcagc gccagcaccc gcgtggacgg caggcccagc gcgtagtcca
     1981 tgtagtcggc catggtcgag acgatctcct gaccgctcga cacgatcagg ttgaacccga
     2041 tgccgcggtc gttgaacgcc accgcggcga acgccgaccc cgagtgcgag atgaacgtca
     2101 ccggcccggc gcgcatgtcg tcaggcgtcg cgaacccggt ggcccgcaag ccggtatcca
     2161 ggttcaggaa gcccatgccg ttgccgccgc acatcgcgat gcccgccgac cgcgcgatcg
     2221 cgccgagccg ctcgggcagc gctggaacgt cggcaggcgg ctcctcctcg aacaacgacg
     2281 agaacgtgac cgccgagcgc gccccggcgg cgacggcatc gcgcaacgcc tgttcgacgc
     2341 gggcgttcga gaccccgagg atcgccagat cgaccggctc gggaagctcc gcgatcgacg
     2401 ggtagcagcg gagtcccagg acctcctcgt agccggggtt caccgggtag atcgagccct
     2461 cgtagcctcc gcgctgcagc tccaccacca tctggttgcc caacgacagc ggtttgaccg
     2521 aggcccccac gacggcgacg gaccgcgcct cgagcatgcg ctcgagcgcg gtccgggtgt
     2581 caccgttcac cgtggattca ggctgcaggg tcggtcaact acttcttggc gacggccttg
     2641 tccttgaccg ccatcttgta ggcctgccac ccggggatgc cgttgacgcc gcccccgccg
     2701 tgcgtggcac acgaaccgtg atacaggcct ctgatgggcg tgcggaagtc cgcgtagccc
     2761 ggcgccggac gcatgtggaa cagctgatcg acgctgagct cgccgtggaa gatgttgccg
     2821 ccgatcaggc ccaggtcctg ctccatgtcg taggggccga tcacctggta gtcgatgatc
     2881 gaatccttga agttcggcgc caggtcgttg tacccgtcga agatccgctg cgcgtaggcg
     2941 tcgagttcgt cgcggtgcgg ctcggtgttc cagtcgtcgg ggacccactg cgtgaacatc
     3001 gagaacacgt gcgtgccttc gggagccagc tgcttgtcga gcgtggtcgg gatcgtgccg
     3061 tcgacgaacg gggcggtggc cggccggcgc tcgatgtgcg cgtcctggaa cgcgcgctcg
     3121 gcatactgcg ggctgaagca cagctccacc gacccggtgt ggtggtcctg ctggttcgtg
     3181 ccggggatcg ccgtgaagtt cggcagctcg ctgatcgcca cgttgacctt caccgtgccc
     3241 gaacgcgtct tccaccgttc gatgtcccac acgaagtcgg cgggcaactc gtgcttgtcg
     3301 agcagatcga ggaacgcgat cttcgggtgg atggtggtca ccaccaccgg cgcacggagc
     3361 tcctcgccgt tctcgagcgc cacgcccacc gccctgccgc cgcgggtcag gatcttcgcc
     3421 accttggcgt cggtgcggat ctcggcgccg aacgattcgg cgctgcggcg gatcgaatcg
     3481 ctcaccgcgc ccatcccgcc ctgctggaac ccccagctgc cgaggtgacc gtcacccacg
     3541 tcgccgatcg agtgatgcag catgacgtat gcggtcccgg gctcgtcggg gccggcccat
     3601 gtgccgatca cgccgttgac cgtgagcatc gccttgatcg catcggactc gaaccagtcg
     3661 ttcagcaggt cggtgacgct catcgtgaac aaccgggtca cgtcggcgac gccgcgctga
     3721 ccgaggccgc gcatcttcca cgcggtctgg gcctgtggca gcaggtcacc gagcttggtg
     3781 gaccccacgt ggggcggcac gctcagcagc agcggaccca acacgtctga gacgcccttc
     3841 atccacgctt cccacttcgg gagggtgtcg gcgtccttct tcgagaactg cgcgatcgag
     3901 tcgtagctct tctgcgagtc gtcggcgtag acggtgatgg cgcgcccgtc ggggaaggcc
     3961 tggaagtagg gaccgaacgg cgtcacgtcg tagccgtgcc gcttcaagtt gagctcgcgg
     4021 atgatcgtcg gcggcatcag cgacatcacg tagctgtagg tggtgacgtt gatgtcgggg
     4081 tgatcggtga acggagcgct ggtatcgg
//
LOCUS       DATDBZ010000219         2878 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_892021, whole genome shotgun sequence.
ACCESSION   DATDBZ010000219 DATDBZ010000000
VERSION     DATDBZ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892021"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(30..93)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01763"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-III (ykkC-III) riboswitch; guanidine
                     riboswitches control genes whose products are involved in
                     modification or pumping out guanidine as a toxic compound
                     from bacteria; this is a third class of guanidine
                     riboswitches based on ykkC-III RNA motif; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF01763"
     gene            complement(151..1053)
                     /locus_tag="VIX62_05450"
     CDS             complement(151..1053)
                     /locus_tag="VIX62_05450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY5859710.1"
                     /translation="MPAHPAVVLGVRPTVSVGLIPALLVCLAAPAFFVVRIPWVGWAL
                     LAAGMLLAWMVRGRTDAEAQAARASVARPAELAETRTPSLLRDLSLIALGLLIVSSIP
                     LAAELDNLAFVRFTLGLGGAVLAPYLVSRFVFRDHAIRFPWRGGGRWTAFQWTWLVAV
                     LAFGWLILPFYFITSGVYMNWPVVDTPEMIARLFVGVGAVGIWDELFFICIVFTLLRR
                     HFPDWQANLLQATVFVSFLWELGYQAWGPLLTIPFALVQAYIFKRTRSLTYVVTVHLL
                     FDAVVFLVLVHAHNPGLLDGLFLI"
     gene            complement(1058..2404)
                     /locus_tag="VIX62_05455"
     CDS             complement(1058..2404)
                     /locus_tag="VIX62_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859711.1"
                     /translation="MTEPEEAASVAPEDAGGALVAPDDGGVATADRDRWRFFRPLAHR
                     DFRALFAAVVLSIFAAGMWAVVMVYAVIAVGGGPLELSLVAASNATGLLVCAIPGGIV
                     ADRVSRRLIVRAVSLTDFLAITSVVIVGFSGPVGIPQLVIVAFVLGAGAGFFFPAYSA
                     ILPRILPPGQLLAANGLEGAIRPGLQQAAGPAVAGMLVAALVPAHAAIVIAIAHGAAF
                     VILLFLRPEPAGEGGEHGEASAKSVFHDLREAVVFTVRTPWLLWTLLYATLWVLVAVG
                     PEEVLLPFLIRERIGDDPRLFGFLLAVYGLGGVLGSIFVSSRKLPRRYLTTMNLVWGF
                     STLPFIIVGFTDQYWLLLASMFTIGFGFSYGNVIWGTLLQRRVPRQMLGRVSSLDFFV
                     SLALMPLSMALAGPLSEVLSLQVIFTAAGVLTLVFGIIAIFAARMPQDEIAHPLTD"
     gene            complement(2401..>2878)
                     /locus_tag="VIX62_05460"
     CDS             complement(2401..>2878)
                     /locus_tag="VIX62_05460"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEY5859712.1"
                     /translation="SAHPEVDFLAVEVFTAGLARTMLNAEKAAVENLRLVEANAPEVL
                     EHLLPPASVDELWVFFPDPWHKSKHYKRRLVTPEFAAIASGALRDGGILRLATDWEQY
                     ARQMRTVLDDAPAFSRSFEGEWAERFDGRVLTAFERKGARAGRAIRDLAYTREERG"
BASE COUNT          552 a          979 c          996 g          351 t
ORIGIN      
        1 aggatgaccc acgacacggg actcgcctcc acggccagtc ttgtcgctca ccgggtactg
       61 atccgtcgtc cggggaccct gttcgagatc ctcgatcagt ttagcaatcc ggcctgtgca
      121 cctcctgggc agcccggccc ggatgccagg tcagatcagg aacaggccgt cgaggagccc
      181 cgggttgtgc gcgtgcacca gcaccaggaa cacgaccgcg tcgaacagca ggtggacggt
      241 cacgacgtag gtcagcgacc gtgtccgctt gaagatgtag gcctgcacca gggcgaacgg
      301 gatcgtcagc agcggtcccc atgcctgata gccgagttcc cataggaacg acacgaacac
      361 cgtcgcctgc agcaggttcg cctgccagtc ggggaaatgg cggcgcagca gggtgaagac
      421 gatgcagatg aagaacagct cgtcccagat gccgaccgcg ccgacgccga cgaacaggcg
      481 cgcgatcatc tccggggtgt ccaccaccgg ccagttcatg tagacgccgg aggtgatgaa
      541 gtagaagggc aggatcagcc agccgaaggc gagcaccgcg accagccacg tccattggaa
      601 cgcggtccag cgtccgcctc cgcgccaggg gaagcggatc gcgtggtccc ggaacacgaa
      661 ccgcgacacg aggtacggcg cgagcaccgc gccgcccagg ccgagggtga accgcacgaa
      721 cgcgaggttg tcgagctcgg ccgcgagggg gatcgaactg acgatcagca gaccgagtgc
      781 gatcagcgag aggtcgcgca gcagactcgg cgtgcgtgtc tcggccagct ctgccggccg
      841 cgccaccgac gcgcgcgccg cctgggcctc ggcatccgtt cgtccgcgca ccatccacgc
      901 gagcagcatc cccgcggcca gcagagccca cccgacccac gggatgcgca cgacgaagaa
      961 cgcgggagcg gcgaggcaga ccagcagggc cgggatcagc ccgacggaga ccgtcggacg
     1021 gacaccgagc acgacggcgg gatgggcggg cacccgttca gtctgtcagc ggatgcgcga
     1081 tctcgtcctg cggcatgcgc gccgcgaaga tcgcgatgat cccgaaaacg agcgtgagca
     1141 cccctgccgc ggtgaagatc acctgcagcg agagcacctc cgagaggggt cccgcgagcg
     1201 ccatcgacaa cggcatgagc gcgagcgaca cgaagaagtc gagactcgag acgcggccga
     1261 gcatctggcg tgggactcgg cgctgcagca gggtgcccca gatgacgttc ccgtagctga
     1321 agccgaaacc gatcgtgaac atcgaggcca gcagcagcca gtactgatcg gtgaagccca
     1381 cgatgatgaa cgggagcgtg ctgaagcccc acaccagatt catcgtggtc aggtagcgcc
     1441 gcggcagttt tctggacgag acgaagatcg atccgagtac gcctccgagg ccgtagacgg
     1501 cgaggaggaa cccgaacagg cgcggatcat cgccgatccg ctcgcgaatg aggaacggga
     1561 gcagcacctc ttcgggcccg accgccacga gcacccacag cgttgcgtac agcagggtcc
     1621 acagcagcca cggtgtgcgc accgtgaaca cgaccgcctc gcgcaggtcg tggaacacgc
     1681 tcttggccga cgcctcgcca tgctcgccgc cttcgcccgc cggctcggga cgcaggaaca
     1741 gcaggatcac gaaggcggca ccgtgcgcga tcgcgatcac gatcgccgcg tgggccggaa
     1801 cgagcgcggc caccagcatt ccggcgaccg ccggccccgc ggcctgctgc agccccgggc
     1861 ggatcgcgcc ctcgagcccg ttcgcggcga gcagctgacc cggcgggagg atgcgcggca
     1921 ggatcgcgct gtaggccggg aagaagaatc cggcgccggc tccgagcacg aaggcgacga
     1981 tcacgagttg agggatgccg accgggcccg agaatccgac gatgacgacg gaggtgatcg
     2041 cgaggaagtc ggtcagcgac accgcgcgca cgatcagacg gcgagagacc cggtcggcga
     2101 cgatgccgcc gggtatcgcg cacacgagca ggccggtcgc gttcgaggcc gccacgagcg
     2161 acagttcgag cggcccgcca cccaccgcga tcacggcgta gaccatcacc accgcccaca
     2221 tgcccgccgc gaagatcgac aggacgaccg cggcgaacag cgcgcggaaa tcgcggtgcg
     2281 cgagcggccg gaaaaagcgc cagcggtccc ggtcggcggt ggccacgccg ccgtcgtcgg
     2341 gtgcgacgag cgcgcctccg gcgtcctcag gtgcaacaga tgccgcctct tcgggctcgg
     2401 tcatccgcgc tcctcgcgcg tgtaggcgag gtcgcggatc gcgcgcccgg ccctggcacc
     2461 cttgcgctcg aaggcggtga gcaccctgcc gtcgaaccgc tccgcccatt caccctcgaa
     2521 ggaccgtgag aacgcgggtg cgtcatccag cacggtgcgc atctgccgcg cgtactgctc
     2581 ccagtcggtc gcgagccgca agatgccccc gtcacgaagg gctccggacg cgatcgcggc
     2641 gaactcgggg gtgaccaggc ggcgcttgta gtgcttgctc ttgtgccacg ggtcggggaa
     2701 gaacacccag agctcatcga cggatgccgg tggcaggagg tgctcgagaa cctcgggcgc
     2761 gttcgcctcc acgagccgca ggttctcgac ggcggccttc tcggcgttca gcatggtgcg
     2821 ggcaagtccc gcggtgaaca cctcgaccgc gaggaagtcc acctcggggt gtgcggat
//
LOCUS       DATDBZ010000220         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2928000, whole genome shotgun sequence.
ACCESSION   DATDBZ010000220 DATDBZ010000000
VERSION     DATDBZ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928000"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VIX62_05465"
     CDS             <1..894
                     /locus_tag="VIX62_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5859713.1"
                     /translation="VEYAIHTTNEDIMGKLTAANGTGFDLVFVSGPFIEPLEGLGYAA
                     EIDQSQIPNMANLDTEATELAFDPGNTHSVPYTWGTTGICYRNDLLSETPSSWEAFHT
                     AAPDTEGKMTMLGTDRWLLQPALLSLGYSVNTTDPAEIDEAKVWTMEAKENLLAFDDT
                     TFYSKLVSGEASLVQAWDGWCEYGRLEDPKNIDFVIPDEGSDLWVDNMVVLEASENKA
                     AAYAFIDYILRPEVGKQIVEYTFYKMPNAEVMAAVDPAVIKGYPTLAITPAELFEQEV
                     QTDLGEEGTILWTETATEIKA"
     gene            914..1810
                     /locus_tag="VIX62_05470"
     CDS             914..1810
                     /locus_tag="VIX62_05470"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007539468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859714.1"
                     /translation="MSTTAAPARRARGRVWGPRIVLLGPGTAWWLVFFLVPVGVLIAY
                     SFFSRGPYGGVIYEPTLKNYVRAFDPLYIGVLWTSVRIATIATGAALLLAYPAALFIA
                     RASSRWRVPLLVLVILPFWTSFLIRTYAWMVLLNPAGLINKVLMGIGVIDEPLPLLYN
                     EFAVTLGLVYAYLPLMVLPIYASLERLGPQSSEAAADLYATPAQTLRKVTIPLTLPGI
                     VAGCIFVFVPSLGNFIVPDLLGGGQTVMIGNVIQQQFLTARDWPFGAVLAMSVIVLMF
                     LVLAVQAQVLRRTQEVEHGRPA"
     gene            1794..2594
                     /locus_tag="VIX62_05475"
     CDS             1794..2594
                     /locus_tag="VIX62_05475"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859715.1"
                     /translation="MAVRRSRYLRVHMGFLFVFLYMPIAVLVVLSFNSSGLPTVWEGF
                     SLQWYRELIGDEELLAAIKNSLIVAGFVTAISVVLGTLLALGLQRTVRSRVLDSALFL
                     PAVIPDIVLAIGLLSFFNLLKFTLGLHTIVIAHVVFDMIFVAAIVRTRLGYFDRRIEE
                     AAADLGANPVTTFLLVTLPAILPGVIAGALVAFTLSIDEFVIAFFNSGPTSITFPIKV
                     YSMIRFGVTPVINAVAAIVMVVSLLTIVIALRLTRPKGETSASLGVSA"
     gene            2591..>2719
                     /locus_tag="VIX62_05480"
     CDS             2591..>2719
                     /locus_tag="VIX62_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5859716.1"
                     /translation="MSRPTNGAKRRVGIKDVAEAAGVSITTVSHALSGKGRLTEETR"
BASE COUNT          418 a          890 c          909 g          502 t
ORIGIN      
        1 gtcgagtacg cgatccacac gacgaacgag gacatcatgg gcaagctcac cgccgccaac
       61 ggcacgggct tcgacctcgt cttcgtgtcg ggtcctttca tcgagccgct cgagggcctt
      121 gggtacgcag ccgagatcga ccagtcgcag atcccgaaca tggcgaacct cgacaccgag
      181 gcgaccgagc tggccttcga tccgggcaac acacactcgg tgccgtacac gtggggcacg
      241 acgggcatct gctaccgcaa cgacctcctc tccgagacgc cgagctcctg ggaagccttc
      301 cacaccgcgg cccccgacac cgaaggcaag atgacgatgc tcggcaccga ccggtggctc
      361 ttgcagccgg cgctgctgag cctcgggtac tcggtcaaca cgaccgaccc ggccgagatc
      421 gatgaggcga aggtgtggac gatggaggcc aaggagaacc tgctcgcctt cgacgacacg
      481 acgttctatt cgaagctcgt ctcgggcgag gcgagcctcg tgcaggcgtg ggacggctgg
      541 tgcgagtacg gacgcctcga ggacccgaag aacatcgact tcgtgatccc cgacgagggc
      601 agcgacctct gggtcgacaa catggtcgtg ctggaagctt ccgagaacaa ggccgccgcg
      661 tacgcgttca tcgactacat cctgcgcccc gaggtgggga agcagatcgt cgagtacacg
      721 ttctacaaga tgccgaacgc cgaggtgatg gccgccgtcg atcccgcggt gatcaagggc
      781 tatccgacgc tcgcgatcac gcccgcggag ctcttcgagc aagaggtgca gaccgacctc
      841 ggcgaagagg gcacgatcct ctggacggag actgccaccg agatcaaggc gtgaggcgct
      901 cgctccaacg gccatgagca cgacggcggc cccggcccgg cgtgcccgtg gccgggtctg
      961 gggtccgcgg atcgtgctgc tcggtccggg cacggcgtgg tggctcgtct tcttcctggt
     1021 gcccgtcggg gtcctgatcg cctactcctt cttcagtcgg ggtccctacg gcggggtgat
     1081 ctacgagccg accctgaaga actacgtccg cgcgttcgat cccctctaca tcggcgtgct
     1141 gtggacctcc gtgcggatcg cgacgatcgc gacgggggcg gccctgttgc tcgcgtatcc
     1201 ggcggccttg ttcatcgccc gtgcctcgtc gcgttggcgc gtgccgttgc tggtgctcgt
     1261 gatcctgccg ttctggacga gcttcctgat caggacgtac gcgtggatgg tgctgctgaa
     1321 cccggccggc ctgatcaaca aggtgttgat gggcatcggc gtgatcgatg agcccttgcc
     1381 actgctctac aacgagttcg cggtgacgct cgggctggtc tacgcctacc tgccgctgat
     1441 ggtgctgccg atctacgcgt cgctcgagcg gctcggcccg cagtcgtcgg aagccgcggc
     1501 cgacctctac gcgacccccg cgcagaccct gcggaaggtc acgatcccgc tcacgctccc
     1561 gggcatcgtc gcggggtgca tcttcgtgtt cgtgccgagc ctcgggaact tcatcgtgcc
     1621 ggacctgctc ggaggcggtc agacggtgat gatcggcaac gtcatccagc agcagttcct
     1681 gacggcgcgt gactggccgt tcggcgccgt gctcgcgatg tcggtgatcg tgctgatgtt
     1741 cctggtgctg gccgtgcagg cgcaggtgct tcggcggacc caggaggtgg agcatggccg
     1801 tccggcgtag ccgctacctg cgggtgcaca tggggttctt gttcgtgttc ctctacatgc
     1861 cgatcgcggt gctcgtggtc ctctcgttca acagctcggg gttgccgacc gtctgggagg
     1921 gcttctccct gcagtggtac cgggagctga tcggcgacga ggagctgctc gccgcgatca
     1981 agaactcgct gatcgtcgcc ggtttcgtca cggcgatatc ggtcgtgctg ggcaccctgc
     2041 tcgcactcgg gctgcagcgc acggtgcgca gccgcgtgct cgacagcgcg ttgttcctgc
     2101 cggcggtgat ccctgacatc gtgctcgcga tcggcttgct ctcgttcttc aacctgctga
     2161 agttcaccct gggcctgcac acgatcgtga tcgcccacgt cgtgttcgac atgatcttcg
     2221 tggctgcgat cgtgcgcacg cggctgggct acttcgaccg ccgcatcgag gaagccgccg
     2281 ccgacctcgg cgcgaacccg gtgacgacgt tcctgctcgt gaccctgccg gcgatcctgc
     2341 ccggcgtgat cgcgggggcg ctggtcgcct tcacgttgtc gatcgacgag ttcgtgatcg
     2401 cattcttcaa ctcggggccg acctcgatca cgttcccgat caaggtgtac tcgatgatcc
     2461 gcttcggggt gacgcccgtg atcaacgcgg tggcggcgat cgtgatggtc gtctcgctgc
     2521 tgacgatcgt gatcgcgctg cggctcacgc gaccaaaggg cgagacgtcg gcgagcctcg
     2581 gggtgagcgc atgagccgac ccacgaacgg cgccaagcgc cgcgtcggca tcaaggacgt
     2641 cgcggaggcc gccggtgtgt ccatcacgac ggtctcgcat gccctgagtg ggaagggccg
     2701 gctcaccgag gagacgagg
//
LOCUS       DATDBZ010000221         2923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1074134, whole genome shotgun sequence.
ACCESSION   DATDBZ010000221 DATDBZ010000000
VERSION     DATDBZ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074134"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /locus_tag="VIX62_05485"
     CDS             complement(<1..1682)
                     /locus_tag="VIX62_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY5859717.1"
                     /translation="MTLAVMGAAMSNRYEERPVGPASAGGLWLQVLGPLRIWRGNVEL
                     DAGPPQQALLLAILLAREGRPVSRNELIDLIWGDDVPQTAHNVIHKYVGVIRRLMEPS
                     LAPRATGSHLVRRGNAYLFLAEPGMLDLGVFRTSVRTAEVARSEQRHDAALAAYLDAL
                     QLWRGEAGAGLDLDPAAMPIFVGLNREFLDACVSAAEVAVSIGRPERLVAPLELAAAM
                     APLHEPVHASLMSILAAAGQQAEALAVHRAIRVRLVEQLGIDPGELLETTYRQVVAQP
                     PARASARLDSPGTVVGSHSSTLGQSGLVGRERDLSLLTAAIEPAFTGASGLAVVEGEP
                     GVGKTRLLEELDVEATRRGARVIWAHCHDGDGTPALWPWVLVVRALLDDRSHAARADW
                     PSAGLGELLDPSTDALTATVFQGGDTQFRMFQRVVDLIGRAAVTRPLVVLLDDLQWAD
                     IATLHLYSHLLGQLPRGVAVVSALRDRAPMPGEDLARALAGVSRAPGHRRLRLAPLDL
                     PDVAELVRNLTDVEPHPDRVRDIHMRTGGNPFFVREVARGDDVRVHSVNDAAG"
BASE COUNT          498 a         1014 c          953 g          458 t
ORIGIN      
        1 cccgcagcat cgttgaccga gtggacccga acgtcgtcgc cccgtgccac ctcgcgaacg
       61 aagaacgggt tgcctccggt gcgcatgtgg atgtcccgaa cccggtccgg atggggctcg
      121 acgtctgtca ggttgcgtac gagttcggca acatcgggca ggtccaacgg cgcgagtcga
      181 aggcgtcgat ggccgggcgc cctgctgacg ccggcgagcg cacgcgccag atcctcgccc
      241 ggcataggcg cccggtcgcg cagggccgac acaacggcga cgccgcgagg caattgcccg
      301 agcagatggc tgtacaggtg gagggttgcg atgtctgccc actgcaagtc gtcgagaaga
      361 acgaccagcg gcctcgtcac ggcggcgcga ccgatgagat cgacgacgcg ctggaacatg
      421 cggaactggg tgtctccgcc ttggaagacg gtcgccgtga gggcatcggt gctgggatcg
      481 agcagctcgc ccagcccggc gctcggccaa tccgctcgcg ccgcgtgcga acgatcgtca
      541 aggagcgctc gcaccactag cacccacggc cagagcgctg gcgtgccgtc accgtcgtga
      601 caatgcgccc agatgacgcg agccccgcgt cgagtggcct ccacgtcgag ttcctccagc
      661 aggcgggtct tgccgacgcc cggttctccc tccacgacgg ccagcccgga cgcgccggtg
      721 aaggccggct cgatcgcggc cgtcagcagc gacaggtcac gctcgcgtcc cacgagtcca
      781 ctctgaccca gcgtcgacga atgggatccg accacagtgc caggcgagtc gagccgggca
      841 gacgctcggg cgggcggctg cgcgacgacc tgacggtagg tcgtctcgag cagctcgcca
      901 gggtcgatgc cgagttgttc gacgagacga acacgtatgg cgcggtgcac cgccaaagcc
      961 tccgcctgtt ggcccgcagc ggcgaggatc gacatcaaac tcgcgtgcac cggctcgtgg
     1021 agcggcgcca tggcggccgc cagctccagc ggcgccacca gccgctcggg cctcccgatc
     1081 gacacggcga cttcggccgc cgacacgcac gcgtcgagga actcgcggtt gaggccgacg
     1141 aagatcggca tcgcggcagg atcgagatcg agccccgcgc cggcttcacc gcgccagagc
     1201 tgtagcgcat cgaggtaggc ggcgagagcg gcgtcatgac gctgctctga tcgcgcgacc
     1261 tcagccgtcc ggaccgacgt gcggaacacg cccaggtcga gcatcccggg ctccgccagg
     1321 aagaggtacg cgttgccgcg gcggaccaga tgggatccgg tcgcccgtgg tgcaagcgac
     1381 ggttccatca atcgccgtat gacccccacg tacttgtgga tcacgttgtg cgccgtctgc
     1441 ggcacgtcat cgccccagat gaggtcgatc agctcgtttc ggctgacggg acggccctcc
     1501 cgcgccagga gaatggccaa caggagtgcc tgctgaggtg ggccggcgtc cagctcgacg
     1561 ttccctcgcc agatcctcag cgggccgaga acctgaagcc acagtccacc ggccgacgcg
     1621 gggccgacgg gtcgctcctc gtatcgattc gacatggcgg ctcccatcac agccagcgtc
     1681 accaggacat cggacgagcc gcgatcctcc cacaccgctg catccaccaa acgctcgatc
     1741 cgacaacggt cccgcgcgaa acgatgctga cggaggtcgc cgcctacgcc gatcagcgtg
     1801 gtgcgtagcc cccggtccgc cgccgcgagc ctcgcgcaag gtcccacgct cggcgaagcg
     1861 ctcagaggtc gggcgaggcg cggtcgcctg ttcgagcccg gtttcttcga gtgagccgat
     1921 cccggctgac actctcgcgt ccacgacgac gcgaggacct gcgctggccc ggtgtgctcg
     1981 tccacatcgg tggcccgttg cagaactgtc aggcgggcat cgttggagaa gtgccgctcg
     2041 ggacacccga taacgcgtgg cgcaatcagt ttgggcaatt cggcccaccc cactcgggtt
     2101 cggccccatt ccggatgccc catatcgagc gcgcagcttg gaggcgtgcc cccaactcca
     2161 accgccggac gtggtcatca ggctcgccgc tgttccgtcg catgggtgcg aggcgcgccg
     2221 tacctgttcg gaggtgatcg acaagtgcag agtcgagccg tgggactcgc acgccctttc
     2281 acgccctcgg tccacttaga gtgcgctgac ctggtggttt gtcgctggac gggggttcgt
     2341 ctcccgaatg ttccccccag catgtctggc tgcgaaaccc caggtcagcg gggttgtgac
     2401 gtcgccagtc gtgcgccgca cggatcacgt cacgacccac aaaacccctg gtagacgggg
     2461 attttcagcg cggtggagct ggggggaatc gaaccctcac aaactcgtcg ctccgaacct
     2521 gctcaggtca cgtgacccca gctcacgccc aattttcagg gtgacacaga cggactggag
     2581 tgaccgttgt tgaccggtgt gacacgccct ctccacgccc cacacgccct tttcgcgccc
     2641 tcgacttgcc gtacgacgga cggtccggaa cctcgacgcc caaacgcgac tcgctcgatc
     2701 cataacacga ttctgagctc gtgacgtgat ggacgacatc acgattctgc tgcttgagcc
     2761 cgtcgaagcg tcctcgaccg gcacatcaat gaggcgacga cgctctccca ggttcgctcg
     2821 ttatgggacg ctcatcggcg actggaacac aggttccgag ccggtctgcg atcgttcgtc
     2881 gccgggaatc ccgagcaggt ctgcgatcgt tcgtcgccgg gat
//
LOCUS       DATDBZ010000222         2142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_686694, whole genome shotgun sequence.
ACCESSION   DATDBZ010000222 DATDBZ010000000
VERSION     DATDBZ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686694"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VIX62_05490"
     CDS             complement(<1..768)
                     /locus_tag="VIX62_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5859718.1"
                     /translation="MIAAAFPALAGVLAQADEVVPLETPDVQWSALWPMIILALGAIL
                     LLTVSSLVKGMRVQGANAAFTVIVGLLSLTACVPVWQRVTDPEQGPFSAVGGAIGIDG
                     FSIMATGLIAIAVILSALLMDNYLRREDMDGAEMYALMLLSASGGALMASANDLIVLF
                     LGLEALSIAVYVMAAMHRRRVESQEAGFKYFIVGAFSSAFFLYGIAMVYGATGSTNLV
                     EIADFLATQILLRNNLLMMGMALLLVGFGFKISAVPFQ"
     gene            complement(765..>2142)
                     /locus_tag="VIX62_05495"
     CDS             complement(765..>2142)
                     /locus_tag="VIX62_05495"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5859719.1"
                     /translation="VVLTAVIFPIALFAATPEHDSKAYYAWLLVLEAGSMGAFLSLDL
                     FLFFVFFEIVLVPMYFLIGAWGHGERVYAAMKFFLYTMFGSAFLLVAIVATAILNAQG
                     TGGAFTFDIVELATSQAIATETARWLFLAFTIAFAIKVPLVPFHTWLPDAHTEAPTAG
                     SVILAAVMLKLGTYGMIRFGLFLFPEAAVYFAPLLLTLGVVGIIYGAVVATVQRDLKR
                     LVAYSSVAHMGFIVLGIFALNTQGLEGGVLQMVNHGISTGALFILVGWIYERRHTRQI
                     SALMGLQKAAPIFAGVFTLVMLSSIGVPGLNGFVGEFLILMGAFASARWWAVIAAVGV
                     ILAAIYLLWAYQRVFHGPIDEENEGFRDLRWTEGLVLAPLLFLIVFLGVYPKPVLERI
                     EPSVTALVAHIEESVPGFSETTGPEPTEDVDLTEVEVPEEGHGDDAEHPEDEMTTDED
                     ATSEEEHE"
BASE COUNT          421 a          665 c          761 g          295 t
ORIGIN      
        1 ctggaacggc acagcggaga tcttgaagcc gaatccgacc agcaacaggg ccatgcccat
       61 catcagcagg ttgttgcgca acaggatctg ggtggcgagg aagtcggcga tctcgaccag
      121 gttcgtggag ccggtggcgc cgtagaccat ggcgatgccg tagaggaaga acgccgagga
      181 gaacgcgccg acgatgaagt acttgaagcc ggcctcctgg gactcgaccc gccgacggtg
      241 catcgccgcc atcacgtaca cggcgatcga cagggcctcg agcccgagga acagcacgat
      301 gaggtcgttg gccgacgcca tcaacgcgcc gccgctcgcc gacaggagca tgagggcgta
      361 catctcggcg ccgtccatgt cctcgcgccg gaggtagttg tccatcaaca gcgcggacag
      421 gatcacggcg atggcgatga ggcccgtcgc catgatcgag aagccgtcga tgccgatggc
      481 tccgccgacg gcgctgaacg gcccctgttc gggatcggtg acccgctgcc acaccggcac
      541 gcaggcggtg agcgacagga gtccgacgat cacggtgaac gcggcgttcg caccctggac
      601 gcgcatcccc ttcaccagcg agctgaccgt gagcaacagg atggcgccga gggcgaggat
      661 gatcatcggc cacagggccg accattggac gtcgggcgtc tcgagcggga cgacctcgtc
      721 ggcctgggcg agaacgccgg cgagcgcggg gaaggcggcg gcgatcactc gtgctcctcc
      781 tcgctcgtgg cgtcctcgtc ggtcgtcatc tcatcctcgg ggtgctcggc gtcgtcgccg
      841 tgtccttctt cgggtacctc gacctcggtg aggtcgacgt cctcggtcgg ttcgggtcca
      901 gtcgtctcgc tgaagcccgg aacggactcc tcgatgtggg cgacgagcgc ggtgaccgac
      961 ggctcgatgc gttcgaggac gggcttggga tagacaccca ggaacacgat gaggaacagc
     1021 agcggcgcga ggacgagacc ctcggtccac cgcaggtccc ggaagccttc gttctcctcg
     1081 tcgatcggcc cgtggaacac ccgctggtag gcccacagga ggtagatggc cgcgaggatc
     1141 acgcccaccg ccgcgatcac cgcccaccag cgagcggagg cgaaggcgcc catgaggatg
     1201 aggaactcgc cgacgaagcc gttgagcccg ggcacgccga tcgacgagag catcacgagg
     1261 gtgaacacac cggcgaagat cggcgccgcc ttctggaggc ccatcagcgc cgagatctgg
     1321 cgggtgtggc gtcgctcgta gatccagccg acgaggatga acagcgcgcc ggtggagatc
     1381 ccgtggttca ccatctggag cacgccgccc tcgaggccct gggtgttgag ggcgaagatg
     1441 ccgagcacga tgaagcccat gtgggccacc gacgagtagg cgaccaggcg tttgaggtcg
     1501 cgctgcaccg tggcgaccac cgcgccgtag atgatgccga cgacgccgag ggtcaacagc
     1561 aacggcgcga agtagaccgc cgcctcaggg aacaggaaca gcccgaagcg gatcatcccg
     1621 taggtgccga gcttgagcat gaccgcggcg aggatcaccg agccggcggt gggtgcttcg
     1681 gtgtgggcgt cgggcagcca cgtgtggaac ggaaccaacg gcaccttgat cgcgaacgcg
     1741 atcgtgaagg cgaggaacag ccagcgggcc gtctcggtgg cgatcgcctg gctggtggcg
     1801 agctcgacga tgtcgaacgt gaacgctccg ccggtgccct gggcgttgag gatcgccgtg
     1861 gcgacgatgg cgacgagcag gaacgccgat ccgaacatcg tgtagaggaa gaacttcatg
     1921 gcggcgtaga cccgctcgcc gtggccccag gcaccgatga ggaagtacat cgggaccagg
     1981 acgatctcga agaacacgaa gaacaagaac aggtcgaggg acaggaacgc ccccatcgat
     2041 ccggcttcga gcaccaggag ccaggcgtag taggccttcg agtcgtgctc gggggtcgcg
     2101 gcgaacagcg cgatcgggaa gatgaccgcg gtgaggacga cg
//
LOCUS       DATDBZ010000223         2746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_686855, whole genome shotgun sequence.
ACCESSION   DATDBZ010000223 DATDBZ010000000
VERSION     DATDBZ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686855"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VIX62_05500"
     CDS             complement(<1..545)
                     /locus_tag="VIX62_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859720.1"
                     /translation="MSSMTVRTHHRARGLGVLGAVAALSVYMVISAVPAWAVVTCAIS
                     GAPPSQTLTVTMAADADTVSLAVAAGNITVTGTGADASCTGPFAKADVSTVLVNAGTP
                     AEVEAQTVNIDGSGDFTASIAVDLGAGNDTVNVQDATGADPISADLGEGSDVFGTTMV
                     DGALSVIGAAGNDDISTGGGN"
     gene            777..1550
                     /locus_tag="VIX62_05505"
     CDS             777..1550
                     /locus_tag="VIX62_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY5859721.1"
                     /translation="MRKEPSAATLVPARDVRIRLLGAFAIWSDGTVPRLPSPAQRLAS
                     FLAMRGEPTARAIAAGTLWPEVGHDHALANLRTALWRLRGAIPGLVHMGQAQLHLDAR
                     ASVDLHRSRTLAERILQGELPMCDGHGSVADLSADLLPDLDDEWLVFERERFRDLRIH
                     ALERLSEQLSAIGEHADAVQCGLLAVEGEPLRESAHRALMRAYIAEENRASALQQFQV
                     LERRLDSELQVGPSIATMELALELRTACEARALGEGRAP"
     gene            complement(1995..>2746)
                     /locus_tag="VIX62_05510"
     CDS             complement(1995..>2746)
                     /locus_tag="VIX62_05510"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY5859722.1"
                     /translation="EAAVTEVLAQRFPDVIPRPIAIDGERGWMLTADFGDQLVATMEP
                     SYWEGSLDALAGLQRRSVASVEFLLESGCQDRRPGRLETQVEELASEGSEWLPDAMLQ
                     RLRAGLSRFHELCEEVASAPIPNTLVHGDLHADNIVISDGDYLIFDWTDACIAHPFID
                     AATFLRYAERASIDQATCEHWRDRYLRGWEDLASYDVISRLFELVEPLTAMHQVITYR
                     WLLDSLDPSERWQFGSAMEDWLTRALAGPEL"
BASE COUNT          426 a          962 c          913 g          445 t
ORIGIN      
        1 tcgttgccgc cgcccgtgga gatgtcgtcg ttgcccgcgg cgccgatcac gctcagcgct
       61 ccgtcgacca tcgtcgtgcc gaacacgtcg ctaccctcgc cgaggtcggc gctgatcgga
      121 tcggcccccg tcgcgtcctg gacgttcacg gtgtcgttgc cggccccgag gtcaaccgcg
      181 atcgaggcgg tgaagtcgcc ggagccgtcg atgttcacgg tctgcgcttc cacctctgcc
      241 ggggtccccg cgttcacgag gaccgtcgag acgtcggcct tcgcgaacgg gccggtgcaa
      301 ctcgcgtcgg cgccggtgcc cgtcacggtg atgttgccgg ccgcgacggc caggctgacc
      361 gtgtccgcat ccgcggccat cgtgaccgtc agcgtctggc tcgggggtgc acccgagatc
      421 gcgcacgtca cgaccgccca cgccggcacg gccgagatca ccatgtagac cgacagcgct
      481 gcgaccgccc cgagcacacc cagcccacgg gctcggtggt gtgtccgtac cgtcatgctg
      541 ctcatccgtt cccccttccg aagggttccg cccgagaggg cggggcggcg tccccggcca
      601 ccggggcgca tcgtcgggcg acgtgcatct ctcgaccgtg tctccgaccg tcatcggagc
      661 gtcgccgggg cgtcagcctg ggttgcgcgc cagggttgtc ctgaggtaga acgatgccgc
      721 acgacgcaac agctggcccg acggcttccc cccacggacc gagagagcgg tggcgcatgc
      781 ggaaggaacc gtcggcggcg accctcgttc ccgcgcgcga cgtccggatc aggctgctcg
      841 gcgccttcgc gatctggagc gacgggacgg tcccgcgtct cccgtcaccg gcccagcggc
      901 tcgcctcgtt cctcgcgatg cgcggcgagc ccaccgcgcg tgcgatcgcc gcgggcaccc
      961 tctggcccga ggtgggccac gaccacgcgc tcgcgaacct tcggacggcg ctctggcggc
     1021 tccggggagc gatccccgga ctcgtccaca tgggccaggc ccagctccac ctcgacgcgc
     1081 gcgcctcggt cgacctgcac cggagccgga cactggccga gcgcatcttg cagggcgagc
     1141 tcccgatgtg cgacgggcac gggtccgtcg ccgacctctc ggcggacctg ctccccgacc
     1201 tcgacgacga atggctcgtg ttcgaacggg aacggttccg cgacctgcgg atccacgcgc
     1261 tcgagcgcct cagcgagcag ctcagcgcga tcggcgagca cgccgacgcc gtccagtgcg
     1321 ggctcctggc ggtcgagggc gagccgctgc gggagtcggc ccatcgcgcg ttgatgcgcg
     1381 cctacatcgc cgaggagaac cgggcgagcg cgctccagca gttccaagtg ctcgaacgcc
     1441 ggctcgactc ggaactgcag gtggggccgt cgatcgcgac gatggaactc gccctcgagc
     1501 tgcgcacagc gtgcgaagcc cgagcgttgg gcgaaggccg cgccccgtga gttcaggagg
     1561 actgggtgct atcacgacga ccgatcgcag ctaaccgtcg gtgatggaac tcagatgcat
     1621 gagagctggg tagcgaactt cggaatcggc gaggtgatga tccagctctt cagcctaagc
     1681 gacagcgaac ggttggccga ggtgcgagtc atgcagtccg ttgccgattg gccccgagcc
     1741 atgcttccgc tctggcccct cccgacgatg ccttacaact ggccgcccga gctggtcatc
     1801 ggcgacccag gtttcgagct tcttaagcgt cgatgggatc aaccaggcgc gcccggctag
     1861 cacaccccat aactccaccc agttcgctcg tcgctgacct gcatcttccc tggtcggcac
     1921 accgctcagc tctcgatagc tggcagccaa gaggtcaggg gttcgactcc ccttgggtcc
     1981 accaccaccg caggtcagag ctcagggcca gccagcgctc tcgtcagcca gtcttccatc
     2041 gccgacccga actgccagcg ttcacttggg tcgagcgaat ccaggagcca tcggtaagtg
     2101 atcacttggt gcatcgcagt gagcggctcg acgagttcga acaggcggct aatgacatcg
     2161 taggaagcga ggtcctccca tccacggagg tagcgatcgc gccagtgttc gcaggtggcc
     2221 tgatcgatcg aggcacgctc ggcgtagcgc aggaacgttg ccgcgtcgat gaatgggtgc
     2281 gcgatgcatg cgtcggtcca gtcgaagatg aggtagtcgc cgtcgctgat gacgatgttg
     2341 tctgcgtgaa ggtcgccgtg gacgagcgtg ttggggatgg gagcgctcgc gacctcttcg
     2401 cagagctcgt ggaaccgtga cagcccagcg cgaagccgtt ggagcatcgc gtccggcagc
     2461 cactcggagc cctccgaggc gagttcctcc acctgggttt ccaacctccc gggtcggcga
     2521 tcctggcatc ctgactcgag caggaactcg acggacgcca cacttctgcg ttggaggccg
     2581 gcgagcgcgt cgagcgatcc ctcccaatac gatggctcca tcgtcgcgac cagttgatcg
     2641 ccgaagtcgg cggtcaacat ccacccgcgc tctccgtcga tcgcgatcgg acgcgggatc
     2701 acatcgggga agcgctgggc gagcacctcc gtcacggccg cttcgt
//
LOCUS       DATDBZ010000224         4530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_893570, whole genome shotgun sequence.
ACCESSION   DATDBZ010000224 DATDBZ010000000
VERSION     DATDBZ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893570"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..501
                     /locus_tag="VIX62_05515"
     CDS             121..501
                     /locus_tag="VIX62_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5859723.1"
                     /translation="MTEPDTLLAGLLDAYIARDAGAMGAVLADDVRVWVTNAEGGVDP
                     LQGRELVIGALLSLEAPVFHIEVTQLVRVSDVQALAMVEIRAERNGKSLHNFSSFLAR
                     TDADRIAELWMVEALPAYSAEFWS"
     gene            508..975
                     /locus_tag="VIX62_05520"
     CDS             508..975
                     /locus_tag="VIX62_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006895546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5859724.1"
                     /translation="MPKAGLTSAGILLWRRRDGALEVLLGHPGGPFFAKKDDWSVLKG
                     EVEPGEDLQAVARREFEEESGTPIAEDAEMVELGEIRQRSGKVVVAWAVEGDLDPATA
                     VSNTFELEWPPRSGRMQKFPEVDRVEWFTLEDARARMRAAQTPFLDRLERAVG"
     gene            1236..1961
                     /locus_tag="VIX62_05525"
     CDS             1236..1961
                     /locus_tag="VIX62_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY5859725.1"
                     /translation="MNPPADEREARIELAAAFRIAVMFDFHEGIANHFSMLIPGRQDR
                     FLINPFGLHFSEITASSLAVVEVDGTPVSGTAPPTGIGIHAPIHRLCPQATVVLHTHQ
                     PWTTALATQEDLHLAPISQTSLQFTDRVVYDERYEGLADLDEGERLAKVLGEAEVLFL
                     RHHGILVAGRELGAAIDDLYMLEKAAQLQVLASGLGPLTPIGDETIERTRDQFADGMI
                     EWGRMHWAALLRMVERTQPDFRD"
     gene            complement(1983..2183)
                     /locus_tag="VIX62_05530"
     CDS             complement(1983..2183)
                     /locus_tag="VIX62_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859726.1"
                     /translation="MNCGHATCRCTIADGEDFCSDHCRDQAAAAGGITDHDSHACECG
                     HPACQGAEADARTEQDIKEAFE"
     gene            complement(2210..2818)
                     /locus_tag="VIX62_05535"
     CDS             complement(2210..2818)
                     /locus_tag="VIX62_05535"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY5859727.1"
                     /translation="MARLFSIRPTLSLKGRRSQGLRGFTGKPFHPPLTDFPIVCYVLA
                     AIFDLISFFAGADDPKAQDFFAAATFVMVAGFIVSLGAALTGFMDWWKGIPRDRKSGP
                     IGKAKHTQVWRTINWHATVMLTVTAITLVDLLLRIPDFDEAQTQALWLVLSCVAAALV
                     AFGAAYGGTLVFEYGFNVENIDEVWNVSEEDHVRPERKKPSA"
     gene            3006..3614
                     /locus_tag="VIX62_05540"
     CDS             3006..3614
                     /locus_tag="VIX62_05540"
                     /inference="COORDINATES: protein motif:HMM:NF033632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HEY5859728.1"
                     /translation="MTERRTRSATPTKARPAPSPAWDDDIERLFESWLRRVAAAEAGH
                     RRESDVMRRGSLMLGIPVVVLTTIAGTSVFASIQRGDVPTGVRVFIGTISILAAVLSS
                     LQTFLRFGMRSEGHRIAAFRYETLRREMIQILALPRAARPEPVTELDHVRLSLDRYAK
                     ESPTIDERRWAKLERQFHLSKVPPDPQWGEHKIRIPEPSSDD"
     gene            3638..>4530
                     /locus_tag="VIX62_05545"
     CDS             3638..>4530
                     /locus_tag="VIX62_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY5859729.1"
                     /translation="MDADLVLEGGGVKGIGLVGAFSTLVDHDYTFHRIAGTSAGAIVG
                     ALIAAGMPPEDLQRVMRSVDYGHFQDEGFVDHLGLVGKGISILFEKGIYEGRYLVEWL
                     DGILMDLGARTFGDLRIEDPNSSLPTERAYKLVIMASDVTRGRLVRLPWDYPRYGLDP
                     DEQLVADAVRASMSIPFFYEPQRFYGRDEHGERLRSFMVDGGMLSNFPIEVFDRTDGK
                     PPRWPTFGIKLSAKPSVPQLQRFEVNGALGLAKAMVGTMTNFHDQMHIDDPSVLARTM
                     FVDTQHVKATDFDLDEPSQDML"
BASE COUNT          755 a         1523 c         1524 g          728 t
ORIGIN      
        1 cgtggccatg taccaaggtt aggaaccgag gggttacgcc ccagacagcc cagaggtaag
       61 agaacggtaa gttgtagccc gcgccacctc cgcttcccgc cttcgtcaag gggatcgacg
      121 atgactgagc cagacacgct gctcgccggc ctgctcgacg cctacatcgc acgcgatgcc
      181 ggagcgatgg gtgccgtgct cgccgacgac gtgcgcgtgt gggtcaccaa cgccgaaggt
      241 ggcgtggacc cgctccaggg gcgtgagctg gtcatcggcg ccctcctttc cctggaggca
      301 ccggtgttcc acatcgaggt gacgcagctg gttcgcgtgt ccgatgtcca ggcgctggcg
      361 atggtggaga tccgggccga gcggaacggc aagagcctgc acaacttctc gtcgttcctc
      421 gcgcgcaccg acgccgaccg gatcgccgag ctgtggatgg tcgaggcgct gcccgcttac
      481 agcgcggagt tctggagctg agccgggatg cccaaggcgg gcctcacgag cgccgggatc
      541 ctgttgtggc gacggcgaga cggtgcgctc gaggtgctgc tcggtcaccc cggtggtccg
      601 ttcttcgcga agaaggacga ctggtcggta ctcaagggcg aggtcgagcc cggcgaggac
      661 ctgcaggcgg tggcgcgccg ggagttcgag gaggagtcgg gcacgccgat cgccgaggac
      721 gccgagatgg tcgagctcgg tgagatccgg cagcggtccg gcaaggtggt ggtggcgtgg
      781 gctgtggagg gcgacctcga ccctgcgacg gcggtgagca acacgttcga gctggagtgg
      841 ccgcctcggt cgggccgcat gcagaagttc cccgaggtcg atcgcgtcga gtggttcacg
      901 ctcgaggacg ctcgcgccag gatgcgcgcg gcgcagacac cgttcctcga ccgactcgag
      961 cgggcggtgg gctaggggcc cgagcggctg ggctgatacc gcaatcggta tgaaccactg
     1021 gcatcggagc gctgagtcgt tccgtatccc ggaatcggta tctacgagcg gcaatggaac
     1081 gctgagtcct tctgcatctc ggaactggta tcaaccgccg ccaggggaac cctgacctcc
     1141 ttccgcatat cggaaacggt gtgggcgacc cccagcagca cgcaccgagc ctggtggcgc
     1201 atactccgag gcatccgagc acggaggccg ccgagatgaa cccacctgcc gacgagcgcg
     1261 aggccaggat cgagctggcc gcggcgttcc ggatcgccgt gatgttcgac ttccacgagg
     1321 gcatcgccaa ccacttctcg atgctgatcc ccggccgcca ggaccgattc ctgatcaacc
     1381 cgttcggcct gcacttctcc gagatcacgg cttcgagcct cgcggtcgtc gaggtcgacg
     1441 gcacgccggt cagcggcacg gcgccgccca ccggcatcgg catccacgcc ccgatccacc
     1501 gcctgtgccc gcaagcgacc gtggtgctac acacccatca accgtggacg acggcgctcg
     1561 cgacgcaaga ggacctgcat ctggcgccga tcagccagac ctccctgcag ttcaccgacc
     1621 gcgtggtcta tgacgagcgg tacgaagggc tcgccgatct cgacgaaggt gagcgtctcg
     1681 ccaaggtgct gggggaagcc gaggtgctgt tcctgcgcca ccacgggatc ctggtggccg
     1741 gccgcgagct cggcgccgcg atcgacgacc tgtacatgct cgagaaggcg gcacagctgc
     1801 aggtgcttgc atccggcctc ggcccgctca ctccgatcgg cgacgagacg atcgagcgca
     1861 cccgggatca gttcgccgac ggcatgatcg agtggggccg gatgcactgg gccgcgttgc
     1921 tccgcatggt ggagcgcacg cagcccgact tccgcgacta gcgaccggct agcgcctccg
     1981 ccttactcga acgcttcctt gatgtcctgc tcggttctcg cgtcagcctc ggcaccttgg
     2041 cacgcggggt ggccgcactc gcacgcgtgc gagtcgtgat cggtgatgcc ccccgcggcg
     2101 gccgcctgat cgcggcagtg gtcgctgcag aagtcctcgc cgtccgcgat cgtgcatcgg
     2161 caggttgcgt gtccacagtt catggggtcc tccaggtctg ctcgggaagc tacgccgagg
     2221 gcttcttgcg ctccgggcgg acgtggtctt cctcggacac gttccagacc tcgtcgatgt
     2281 tctccacgtt gaacccgtac tcgaatacca gcgtgccgcc gtaggccgcc ccgaacgcca
     2341 ccaacgcggc ggccacgcat gacaacacga gccacaacgc ctgcgtctgc gcttcgtcga
     2401 agtcggggat ccgcagcagc agatcgacga gcgtgatggc cgtgacggtc agcatcaccg
     2461 tggcgtgcca attgatcgtt cgccacacct gcgtgtgctt ggccttgccg atcggacccg
     2521 acttgcggtc gcgcgggatg cccttccacc agtccatgaa cccggtcagc gccgcgccga
     2581 ggctcacgat gaagcccgcc accatcacga acgtcgccgc cgcgaagaag tcctgggcct
     2641 tcgggtcgtc ggcgcccgcg aagaacgaga tcaggtcgaa gatcgccgcg agcacgtagc
     2701 agacgatcgg gaagtcggtg agcggggggt ggaacggctt ccccgtgaac ccccggagcc
     2761 cctgggaacg gcgtcccttc aggctcagcg tgggacggat cgagaacaac ctcgccatct
     2821 gcgcctcctc gtctcgcggt gaacgagggc ggggaccctc cgcatccttg agcatcatgc
     2881 cacgggaacc cggcgtggtc ggcagacggc gtcggtccgc tgggggagcc gggaatcacg
     2941 agcttggccg actgacccgg ggaccgtcgc tgcctacggt gaggcccgat ataccggagg
     3001 acgtcgtgac cgaacgacga acacgcagcg ccacacccac caaagccagg cctgccccga
     3061 gtccggcctg ggatgacgac atcgagcggc tcttcgagtc ttggctgcga cgtgtggctg
     3121 cggcggaggc cggccaccgc cgcgaatccg atgtgatgcg gcgtggatcc ctgatgctgg
     3181 ggatcccggt cgtcgtcctg accacgatcg ccggtaccag cgtcttcgcc tcgatccagc
     3241 gcggcgacgt tccgacgggc gtccgggtgt tcatcggcac gatcagcatc ctcgccgcgg
     3301 tgctgtcgag cctccagacg ttcctccgct tcggcatgcg atcggagggc catcggatcg
     3361 cggcgttccg ctacgagacg ctgcgccgcg agatgatcca gatcctcgcg ctgccccgcg
     3421 cggcgcggcc cgagcccgtc accgagctcg accacgtacg cctgtccctg gaccggtacg
     3481 cgaaggagtc gcccacgatc gacgaacggc gatgggcgaa gctcgaacgc cagttccacc
     3541 tgtcgaaggt tccgcccgac ccgcagtggg gcgagcacaa gatccggatc cccgaaccct
     3601 catccgacga ctgagccctg ctcaccaagg agtgcatatg gacgctgacc tcgtgctgga
     3661 aggcggtggc gtgaaaggca tcgggttggt cggtgccttc tccaccctgg tcgaccacga
     3721 ctacacgttt caccgcatcg cgggaacatc ggccggcgcg atcgtcggcg cgctgatcgc
     3781 cgccggcatg ccgcccgagg acctccagcg cgtgatgcgc tcggtcgact acggacactt
     3841 ccaggacgaa ggattcgtcg accacctggg cctggtgggc aagggcatct cgatcctgtt
     3901 cgagaagggg atctacgagg ggcgctacct cgtcgagtgg ttggacggga tcttgatgga
     3961 cctcggcgcg cgcacgttcg gcgatctccg catcgaggac ccgaactcct cgctgcccac
     4021 cgaacgggcc tacaagctcg tcatcatggc ctccgacgtc acccgaggca ggctggtgcg
     4081 gctgccgtgg gactacccga ggtacgggtt ggacccggac gaacaactgg tggccgacgc
     4141 ggtgcgcgca tcgatgtcga tcccgttctt ctacgagccg cagcggttct acgggcgcga
     4201 cgagcacggc gaacggctgc gctcgttcat ggtcgacggc ggcatgctgt cgaacttccc
     4261 gatcgaggtg ttcgaccgaa cggacgggaa gccgccccgg tggcccacgt tcgggatcaa
     4321 gctctcggcg aagccgtccg tgccccagct gcagcgcttc gaggtgaatg gggcgctcgg
     4381 tctcgcgaaa gccatggtcg ggacgatgac gaacttccac gaccagatgc acatcgacga
     4441 tccgtcggtc ctggcgcgca cgatgttcgt cgacacccag cacgtgaagg ctaccgactt
     4501 cgacctcgac gaaccctcgc aggacatgct
//
LOCUS       DATDBZ010000225         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_49413,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000225 DATDBZ010000000
VERSION     DATDBZ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49413"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VIX62_05550"
     CDS             complement(<1..188)
                     /locus_tag="VIX62_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859730.1"
                     /translation="MAVAVRTRLIAVVAVAVTVLALAGVGVVRAADAPSLPPITSDRL
                     LASTAQALSQPVTISGDV"
     gene            complement(273..587)
                     /locus_tag="VIX62_05555"
     CDS             complement(273..587)
                     /locus_tag="VIX62_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859731.1"
                     /translation="MKVLEIVLAVLFATLGVRSVIHWVRRPFASQDMTDQVLFALFVT
                     ARAGWWFAASGLFALYATMGEQGDVAVTVRRYDWYLLLLISLAALQFVSGFILGRRTS
                     DR"
     gene            complement(584..1231)
                     /locus_tag="VIX62_05560"
     CDS             complement(584..1231)
                     /locus_tag="VIX62_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY5859732.1"
                     /translation="MRFRDRTEAGELLGAAVRERVSGPAVVLGLARGGVIVAAGVAEA
                     LGASLDVAVARKLGAPGNPELGIGAVAPGVQVVDERLVHLLRVSPRFIEAETEAQSQE
                     VERRLLAYRGDRPSPAMRGATVVVVDDGVATGVTARAALRWVRTEGASRLVFAAPVGP
                     QGACRMLAPDCDECLILHTPTSLRAVGEWYDVFDQTTDDEVRAAIEARDRPDGDA"
     gene            1349..1582
                     /locus_tag="VIX62_05565"
     CDS             1349..1582
                     /locus_tag="VIX62_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY5859733.1"
                     /translation="MVHAYILIQTEVGKAAAVADEVRSIGGVAEAEDVTGPYDVIVRA
                     EAKTVDELGKLVVARIQAVEGITRTLTCPVVHL"
     gene            complement(1609..>2207)
                     /locus_tag="VIX62_05570"
     CDS             complement(1609..>2207)
                     /locus_tag="VIX62_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY5859734.1"
                     /translation="ARVAALGYPVFTKPATLGSSVGILKAHEPAALDACLDEAFRYAR
                     KAVVERGIESVREIECAVLGNDDPVASVCGEIVPKGHEFYDYASKYLDADGAQLVIPA
                     DLSADTAQTVQRMAITAFEAIECWGMARVDFFVRDDEVWINEINTIPGFTSISMYPKL
                     WEASGLAYDDLVERLIDLARERHAVERAKGTVARELEP"
BASE COUNT          367 a          801 c          738 g          301 t
ORIGIN      
        1 tcgacgtcgc cggagatcgt caccggctgc gagagcgcct gcgccgtgga cgccaacagg
       61 cgatcggacg tgatgggcgg cagcgacggc gcgtcggcgg cccgaaccac gccaacccct
      121 gccagcgcga gcaccgtcac ggccacggcg acgacggcga tcaggcgggt gcggacggca
      181 acggccatac ccctttgtaa caccggttcg tgcggaggga cggctcgacc cgagggctgc
      241 acagcttcgt cacaggcgga gccgtcgctg gttcagcggt cactggtcct tcggcccagg
      301 atgaatcccg agacgaactg cagcgccgcc aaggagatca ggagcaggag gtaccagtcg
      361 tagcggcgca ccgtcacggc gacgtcaccc tgctccccca tcgtggcgta caaggcgaac
      421 aaccccgacg cggcgaacca ccacccggct ctcgcggtca cgaacaacgc gaacaacacc
      481 tggtcggtca tgtcctggct ggcgaacggg cgccgcaccc agtggatgac ggagcggacg
      541 ccgagcgtgg cgaagagcac cgccagcacg atctcgagga ccttcacgcg tcgccgtccg
      601 gccggtctcg ggcctcgatc gccgcgcgga cctcgtcgtc ggtggtctgg tcgaacacgt
      661 cgtaccactc tcccacagcc cgcagcgacg tcggggtgtg gaggatcagg cactcgtcgc
      721 agtcgggcgc caacatgcga caggcgccct ggggccccac cggcgcggcg aacaccagcc
      781 gggatgcgcc ctcggtgcgg acccatcgga gtgcggccct cgcggtgacg ccggtggcca
      841 cgccgtcgtc gaccacgacg acggtcgcgc cccgcatcgc gggcgagggc cggtcacccc
      901 gataggcgag caggcggcgc tccacctcct gtgactgagc ctcggtttcg gcctcgatga
      961 accgcggcga cacgcgcagc agatggacca gacgctcgtc cacgacctgc accccgggag
     1021 ccaccgcacc gatgccgagt tccgggttcc cgggagcacc cagcttcctg gcgacggcga
     1081 cgtcgagcga tgcgccgagg gcctcggcga cacccgcggc cacgatcacc ccgccgcgag
     1141 cgaggcccag gaccacggcg ggacccgaca cgcgctcgcg gacggcggcg cccaacagct
     1201 cacccgcttc ggttcggtcg cggaacctca tcacgccgcg atgatgcgcg ttgcgtacgc
     1261 tcgacgtcca cggctagcat gggcgccctc gtgcacctgg ttcaccgcgc gggacataga
     1321 gacgaggtcc gatgagggag gttcaccggt ggtgcacgcg tacatcctga tccagacgga
     1381 ggtcggcaag gccgccgccg tcgccgatga agtgcggtcg atcggtggcg tcgccgaggc
     1441 cgaggacgtc accggcccgt acgacgtgat cgttcgcgcc gaggccaaga ccgtggacga
     1501 gctgggcaag ctggtcgtcg cccgcatcca ggcggtcgaa ggcatcacgc gaaccctcac
     1561 ctgcccggtg gtgcacctct agtccatcgg acgaccggac ccggcgcctc agggttcgag
     1621 ttcccgggcg accgtgccct tcgcgcgttc gacggcgtgg cgctccctcg cgaggtcgat
     1681 cagccgctcc accaggtcgt cgtaggccag cccgctcgcc tcccacaact tcggatacat
     1741 ggagatcgac gtgaaccccg ggatcgtgtt gatctcgttg atccacacct cgtcgtcccg
     1801 cacgaagaag tcgaccctgg ccatgcccca gcactcgatc gcctcgaacg ccgtgatcgc
     1861 catccgctgg accgtctgtg cggtgtcggc ggacaggtcg gccgggatca ccagctgggc
     1921 gccgtcagca tcgaggtact tcgatgcgta gtcgtagaac tcgtggccct tgggcacgat
     1981 ctcaccacac accgaggcca ccggatcgtc gttgcccagc acggcgcact cgatctcgcg
     2041 caccgactcg atgccgcgtt ccaccacggc cttgcgggcg tagcggaacg cctcatccag
     2101 acaggcgtcc agggccgccg gttcgtgggc cttcaagatc ccgaccgatg accccagggt
     2161 tgccggcttc gtgaagaccg ggtagccgag cgccgccacg cgggcgg
//
LOCUS       DATDBZ010000226         4903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1075906, whole genome shotgun sequence.
ACCESSION   DATDBZ010000226 DATDBZ010000000
VERSION     DATDBZ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075906"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VIX62_05575"
     CDS             <1..530
                     /locus_tag="VIX62_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5859735.1"
                     /translation="QETMPDVVLMDVRMPKRSGIEAAGQIKELLPHCKILMLTISDEE
                     ADLYDAIKAGASGYLLKEIPIEEVADAIRSVWAGQSRISPSMASKLLTEFAAMSKSAD
                     ERPQMPAPRLTDREMEVLRLVAQGLNNRDIAKQLFISENTVKNHIRNILEKLHLHSRM
                     EAVVYAVREKMIEIS"
     gene            534..1016
                     /locus_tag="VIX62_05580"
     CDS             534..1016
                     /locus_tag="VIX62_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5859736.1"
                     /translation="MTDPTIDAAAQLLGDSLDLLRATIDGLPSAALDEQPSGPDTNSL
                     AVLVTHSLHSTRAWLSVAVAAPLPDRDRPAEFAASAADLDAFLAGFDSMSNDCRALLE
                     HAGPFDPGRVGTAPWRPGDLAQEPVTAAWALIHALVHLREHVGHAELTRQLWDERPGA
                     "
     gene            1044..2699
                     /locus_tag="VIX62_05585"
     CDS             1044..2699
                     /locus_tag="VIX62_05585"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEY5859737.1"
                     /translation="MSSSRPQRTVARLIVDCLENEGVEYVFGLPGEENLHMIDALTDS
                     SIRFVTTRDERGAAFMADTYGALTGKPGVCLATLGPGAINLMLGIANAQLDSHPMLAI
                     TAQAGLHRIYKESHQFVDLVSLFRPITKWGDMVTVRDAAPEMVRKAFKQATTERPGAT
                     FLILPEDVAERETGATPLRVNTPVDPSPDENQVHRVAHVLASAKHPVVLAGPGVARDG
                     ATDALIRFAERLNLPVATTFLGKGVFPDNHPNALGTIGFMVKDYANFGFDRADVVVAV
                     GYDLVEYSPERWNPNADKRIIHVHRTVAEVDANYTLEVGVQGNISETLDAIGGSAYIH
                     DIRGQIPPVRDLVHEELERGAGDDAFPLAPARIVHDVRQALGDDDIVLCDTGAVKMWM
                     ARLYPTYRPNTCLISNGLATMAFSLPGALAAKLVHPERRVVAAMGDGAFAMSMSEIET
                     AVREGIHFVVLVWVDGSYGLIGWKQDIHFGRQSAVSFTNPDFIKLAESFGAKGYEIGA
                     ASELAPTLEKALADEAVSIIACPVDYRENARLIERLGALTESI"
     gene            2803..>4903
                     /gene="secA"
                     /locus_tag="VIX62_05590"
     CDS             2803..>4903
                     /gene="secA"
                     /locus_tag="VIX62_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY5859738.1"
                     /translation="MPAVLDKISNMGEGRKLKNLTALAKLVNTFEPETEDLTDDELRA
                     KTPEFRQRAANGESLDDMLPEAFAVVREASRRVNGQRHFDVQLMGGAALHQGNIAEMK
                     TGEGKTLVSTLPGYLNAMSDKGVHVVTVNDYLAKRDSEWMGGIYRFLGLNVGLIQATM
                     TPLQRTPAYAADITYGTNNEFGFDYLRDNMAMRLEECVQRGYNFAIVDEVDSILIDEA
                     RTPLIISGQVADSAKWYVTFARIAPRLKRDEDYEVEESKYQVAITEEGVDKVEEILGI
                     ENLYEHANTTLVHHLQNSLRAKELYKRDVAYVVTDGEVKIVDEFTGRVLEGRRYSEGL
                     HQAIEAKEGVKIKEENQTLATITIQNYFKMYDKLSGMTGTAKTQLTEFEETYKISVVE
                     MPTNRPNVRNDEQDLIFKSETAKWNAVADDIIERNEAGQPVLVGTVSIEKSEVLSTVL
                     NRRGVQHNVLNAKNHEKEAVTVAQAGRKGGVTVATNMAGRGVDILLGGNPEYLARQEM
                     AARGFDNDAYLLFELDEDERAAYEAEYEPIFAKLRSQTDAEHDEVVGLGGLYVLGTER
                     HESRRIDNQLRGRAGRQGDPGESRFYLSLEDDLMRMFASDKVASIMERLKWPDDEAIE
                     AKMVSRAVENAQKQIEELNYERRKNVLKYDEVVELGGLFVLGTERHESRRIDNQLRGR
                     SGRPGDPGESPVYLSLGD"
BASE COUNT          933 a         1639 c         1621 g          710 t
ORIGIN      
        1 cgcaagagac catgcccgac gtcgtgctga tggatgtgcg catgcccaag cgctccggga
       61 tcgaagcggc cggccagatc aaggaactcc tgccgcactg caagatcctg atgctcacga
      121 tcagcgacga agaggccgac ctgtacgacg cgatcaaggc gggcgccagt ggctacctcc
      181 tgaaggagat ccccatcgaa gaggtcgccg acgcgatccg cagcgtgtgg gcgggccagt
      241 cgcggatctc accgtcgatg gcgtcgaaac tgctcaccga gttcgcggcg atgagcaagt
      301 ccgccgacga gcgcccgcag atgccggcgc cccgcctcac cgaccgcgag atggaggtgc
      361 tccgcctcgt cgcgcaggga ctgaacaacc gcgacatcgc caagcagctc ttcatcagcg
      421 agaacacggt gaagaaccac atccgcaaca tcctcgagaa actgcacctg cattcgcgca
      481 tggaggcggt cgtctacgcc gtgcgcgaga agatgatcga gatcagctga accgtgaccg
      541 atccgacgat cgacgccgca gcacagctct tgggcgattc gctcgatctg ctccgtgcca
      601 cgatcgacgg cctaccctcg gcagccctcg atgaacagcc cagcggcccc gacacgaact
      661 cgctcgccgt cctggtgacg cactcgctgc acagcacccg ggcgtggctc agcgtggccg
      721 tggccgcacc gctgcctgac cgtgaccggc cggccgagtt cgccgcctcg gccgccgacc
      781 tcgatgcgtt cctcgccggg ttcgattcca tgtcgaacga ctgccgtgcc ctgctcgagc
      841 atgcgggtcc cttcgatccg ggacgggtcg ggaccgcgcc gtggcgcccc ggagacctgg
      901 cgcaggagcc cgtcacggcc gcctgggcgc tgatccacgc cttggtgcat ctgcgtgagc
      961 acgtggggca cgcggagctg acgcgccagc tctgggacga acgacccggc gcctagcgtt
     1021 cacgggtccg ataccctcga cgcatgagtt cctcgcgtcc ccagcgcacc gtcgctcgcc
     1081 tgatcgtcga ctgcctcgag aacgaagggg tggagtacgt cttcggcctg ccgggcgagg
     1141 agaacctcca catgatcgac gccctgacgg actcctcgat ccggttcgtc accacgcgtg
     1201 atgaacgcgg ggccgccttc atggccgaca cctacggggc gctgaccggg aagccggggg
     1261 tgtgcctggc caccctgggg cccggagcga tcaacctgat gctggggatc gcgaacgccc
     1321 agctcgactc ccacccgatg ctggccatca cggcgcaggc cggtctgcat cgcatctaca
     1381 aggagtccca ccagttcgtc gacctggtgt cgctgttccg gcccatcacg aagtggggcg
     1441 acatggtgac cgtgcgcgac gcggcgccgg agatggtgcg caaggccttc aaacaggcga
     1501 ccaccgagcg gcctggcgcg accttcctga tcctgcccga ggacgtcgcc gagcgcgaga
     1561 cgggtgcgac accgctgcgg gtcaacacgc cggtcgaccc ttcgccggac gagaaccagg
     1621 tgcaccgtgt cgcccacgtg ctggcgtcgg cgaagcaccc cgtggtcctg gccggccccg
     1681 gggtggcccg cgacggcgcc accgacgcgc tgatccggtt cgccgaacgg ctcaacctgc
     1741 cggtggccac cacgttcctg ggcaagggcg tgttccccga caaccatccg aacgcgctgg
     1801 gcacgatcgg gttcatggtg aaggactacg cgaacttcgg gttcgaccgt gccgacgtgg
     1861 tggtggcggt gggctacgac ctcgtcgagt actcgcccga gcgctggaac ccgaacgcgg
     1921 acaagcggat catccacgtg catcgcacgg tggccgaggt cgacgcgaac tacacgctgg
     1981 aggtgggcgt gcagggcaac atcagcgaga ccctcgacgc gatcggtggt tcggcctaca
     2041 tccacgacat ccgcgggcag atcccgcccg tccgcgacct ggtgcacgag gaactggaac
     2101 gcggcgcggg cgacgatgcc ttcccgttgg cgccggcgcg catcgtgcac gacgtccgcc
     2161 aggcgctggg ggacgacgac attgtgctgt gcgacacggg cgcggtgaag atgtggatgg
     2221 cgcgtctgta cccgacgtat cgccccaaca cctgcttgat ctccaacggc ctggcgacga
     2281 tggcgttctc gctcccgggg gcgctggccg ccaaactcgt ccatcccgag cgccgggtcg
     2341 tggccgccat gggcgacggc gcgttcgcga tgagcatgag cgagatcgag accgcggtgc
     2401 gggaggggat ccacttcgtg gtgctggtct gggtggacgg gagctacggc ctgatcgggt
     2461 ggaagcagga catccacttc ggccgtcagt ccgccgtgag cttcaccaac ccggacttca
     2521 tcaagctggc ggagagcttc ggcgccaagg gttatgagat cggtgccgcg agtgagctgg
     2581 cccccaccct cgagaaggcc ctggccgacg aggcggtctc gatcatcgcc tgcccggtcg
     2641 actatcgcga gaacgcccgg ctgatcgagc ggctcggcgc tctgaccgag tccatctgac
     2701 cgagaccgcc gcaggtcaga ggccgtcctc gaggtcctga accagcgggc cgatagaatc
     2761 ggatacccga caccgtcaac ggactccccc gaaaggggcc gcatgccagc cgtactcgac
     2821 aagatctcga acatggggga agggcgcaag ctcaagaacc tcaccgccct cgccaagctc
     2881 gtcaacacct tcgaacccga gaccgaagac ctcaccgacg acgaactgcg ggccaagacc
     2941 ccggagttca ggcaacgggc ggcgaacggc gagtcgctgg acgacatgct ccccgaggcc
     3001 ttcgcggtgg tgcgcgaggc ctcccgccgc gtcaacggtc agcgccactt cgatgtgcag
     3061 ctcatgggtg gggcggccct gcaccagggc aacatcgccg agatgaagac cggtgaaggc
     3121 aagacgctgg tgtccaccct gcccggttac ctcaacgcga tgtcggacaa gggcgtgcac
     3181 gtcgtcaccg tcaacgacta cctcgccaag cgcgactccg agtggatggg cgggatctac
     3241 cgcttcctcg gactgaacgt gggtctgatc caggcgacga tgacgccgct gcaacgcacg
     3301 cctgcctacg ccgccgacat cacctacggc acgaacaacg agttcgggtt cgactatctg
     3361 cgcgacaaca tggcgatgcg gctcgaggaa tgtgtccagc gcggctacaa cttcgcgatc
     3421 gtggacgagg tcgactcgat cctgatcgat gaggcacgca cgccgttgat catcagtggg
     3481 caggtggccg actcggcgaa gtggtacgtc acgttcgcgc ggatcgctcc gaggctgaag
     3541 cgtgacgagg actacgaggt ggaggagtcc aagtaccagg tcgccatcac cgaagaaggc
     3601 gtggacaagg tcgaggagat cctcggcatc gagaacctct acgagcacgc caacaccacg
     3661 ctggtccatc acctgcagaa ctcgctgcgc gccaaggagc tctacaagcg tgacgtcgcc
     3721 tacgtggtga ccgacggcga ggtcaagatc gtcgacgagt tcaccggtcg cgtgctggag
     3781 ggccggcgct actccgaggg gctgcaccag gcgatcgagg ccaaagaagg cgtcaagatc
     3841 aaggaagaga accagacgct ggccacgatc acgatccaga actacttcaa gatgtacgac
     3901 aagctctcgg gcatgacggg taccgccaag acccagctca ccgagttcga ggagacctac
     3961 aagatctccg tcgtcgagat gcccacgaac cgcccgaacg tccgcaacga cgagcaggac
     4021 ctgatcttca agagcgagac ggcgaaatgg aacgccgtcg ccgacgacat catcgagcgg
     4081 aacgaggccg gtcagcccgt cctggtgggc accgtctcga tcgagaagtc cgaggtgctg
     4141 tccacggtgt tgaaccggcg cggcgtccag cacaacgtcc tgaacgccaa gaaccacgag
     4201 aaggaagccg tcaccgtcgc ccaggccggg cgcaagggcg gggtcaccgt ggcgacgaac
     4261 atggcgggcc gcggcgtcga catcctgctg ggtggcaacc ccgagtacct cgcccgccag
     4321 gagatggccg cgcgcggatt cgacaacgac gcctatctgc tgttcgagct ggacgaggac
     4381 gaacgcgcgg cctacgaggc cgagtacgag ccgatcttcg cgaagctgcg ctcgcagacc
     4441 gatgccgaac acgacgaagt ggtggggctg ggcgggctct acgtgctggg caccgaacgg
     4501 cacgagtccc ggcggatcga caaccagctg cgtgggcgtg ccggccgcca gggtgacccc
     4561 ggcgaatccc ggttctacct ctcgctcgag gacgacctca tgcggatgtt cgcctcggac
     4621 aaggtcgcat cgatcatgga gcggctgaag tggcccgatg acgaggccat cgaagccaag
     4681 atggtctcgc gcgccgtgga gaacgcgcag aagcagatcg aagaactgaa ctacgagcgc
     4741 cgcaagaacg tcctgaagta cgacgaggtg gtcgagctgg gcggactgtt cgtgctcggc
     4801 accgagcggc acgagtcacg acgcatcgac aaccagctcc gcggccggtc gggtcgccca
     4861 ggcgacccag gcgagtcccc ggtctacctc tcgctcggcg acg
//
LOCUS       DATDBZ010000227         5115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1506837, whole genome shotgun sequence.
ACCESSION   DATDBZ010000227 DATDBZ010000000
VERSION     DATDBZ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506837"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..902
                     /locus_tag="VIX62_05595"
     CDS             435..902
                     /locus_tag="VIX62_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY5859739.1"
                     /translation="MRDRRATKRVRRGIAGGERGAAAIEFALVAPLLFLLVFGIIDFG
                     FGFHAWDASHNAAREGARVGAVNPNPTLIEARARAASDFLDQSLIDVTVQCAAEGSTS
                     FGACGDGSGWVEGDVVRVTVIYDYNYMTPLPNIVGLGDSLAVKGISESRFEGQ"
     gene            902..2281
                     /locus_tag="VIX62_05600"
     CDS             902..2281
                     /locus_tag="VIX62_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5859740.1"
                     /translation="MAKARQPGRIRGLIKDRLHDESGAVVIISALLIVVLMGSVAFAV
                     DLARLRHERQVLQTAVDLGALAGAGKLPAQGGDAAADAVATATRIAIANAPQLASATL
                     DISFRCAVADPEGNGGADSQDVKFACGPDGGGGWIDGWNTRRGRAFHSCDPWAGDLCN
                     TIVVRASNLVPYFFAPVLGIDQGSTGTVSGASCKGFCGQPSSPLDVAMVLDRSTSMTN
                     ADMANLKNAALSVLDFYDPALQHVALVALPYTNSTNRCIANPTQNYPAVGRMWQVTGL
                     SSDYKSGGSLNTSSELVTRINCLQRATNLTSVRPSGGHTDLGDPMATAHDIVVNDGRP
                     DVPDVIIFMTDGEANQPRGLQPCDYFVDRAIPAQDDEVNVYVIAYGVASARCGEDTTG
                     PYRGDYASTSAADAASGDSDDNAPGGCASDENTDGDYYFCESGSSDLEPVFRQIAVAA
                     LERSRLIDF"
     gene            2334..3122
                     /locus_tag="VIX62_05605"
     CDS             2334..3122
                     /locus_tag="VIX62_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY5859741.1"
                     /translation="MSDAWIRALVALPFGLAIGSFMTVVVSRVPAGESVLRPRSRCTS
                     CGTEIRNRDNVPVLSWVLLRGRCRSCGERISARYPLLELSTASLVVAAAAAYEQIWLA
                     AMIAAFLSLMPAVAWIDIEHRIIPNRITYPAFLGFSAYVVVAWLFDGGTDPVRALAGA
                     LIYGGLLFVVALVSRGMGMGDVKLALVIGVVLGAVGLRYVGVAAAGAVLFGGIGGIVA
                     LIVGRNRKAMIPFGPYMAAGAVVGAFWGEPIAEWYLRSFSATAL"
     gene            3282..3488
                     /locus_tag="VIX62_05610"
     CDS             3282..3488
                     /locus_tag="VIX62_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5859742.1"
                     /translation="MADAQPFVTGALLTVAEVAAGMRVSNMTVYRLIKSGELPAVRVG
                     KGYRIRESELQRFLEERSVQVEGG"
     gene            3488..4588
                     /gene="pilM"
                     /locus_tag="VIX62_05615"
     CDS             3488..4588
                     /gene="pilM"
                     /locus_tag="VIX62_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEY5859743.1"
                     /translation="MAKGRIGLDIGSTAVRAVELTGGDPPTVIKAAQVPLPAGAVENG
                     EVKDPDVVTEALRELWERGGFKAKRVYMGVGNQRVVVREIALPYLPEKELRASLGFQV
                     QEFIPMAVDEAVLDYDPIGEFEQDDRKMLRMLLVAAQRQMVDTVVSAATGAKLEPVGL
                     DLVPFAMVRSVGTTGVGMDLEDEGDEAVIDIGAHVTNIVVHARGTVRFVRILPSGGRD
                     VTLAIARGIGVEDDVAERLKRGEDVELETPPRVDQEGGNEVAEPPDTGRVKEIAMQRA
                     ASFVDEIRSSLEFYTAQAQGARIARVLVTGGGSKLEGFLELLRQRIPVEVEAGQVFQH
                     ARSQLSLSDEALAEAEPVLAVAVGLAIPGRNA"
     gene            4585..>5115
                     /locus_tag="VIX62_05620"
     CDS             4585..>5115
                     /locus_tag="VIX62_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859744.1"
                     /translation="MSQVNLLPPDILQAQRYRRLTSGVVVAGVVLVAAVFGYYLLQSN
                     ELSSVNDEIAAQEATNASIQGSIAEKQKYADLQAEAQAQQQLLSAAYAGEVSFSALLM
                     DFSRVIPSDAYVNSLAIQADQAAGGTEGATPGLIGGITGSGQAVSIDTLSVFLTRLEQ
                     VKGWVNPWMSTVSKNEE"
BASE COUNT          792 a         1689 c         1811 g          823 t
ORIGIN      
        1 gggtcggcgg aatgcggcgg caacccgccg ccgaactcga gcgcccactg cttggtcctg
       61 aagtggaagg gcgccaggat cggtggttcg gagccaggcg gcggcgccaa cttcggcctg
      121 ctcgcggcgc agctgtgcga cctcgcgtac ggcagctgcc tcgaatagca cgatctgctt
      181 cctcggcagc cgagcccccc tccgctgtgg tcgatgtgat caacgattcg tcggagcagt
      241 cgttcgcgga cgaagggctg agcccgcaga cccggccagc cgatgcctcg gagggaaatg
      301 tcgtacggcc aacgactcaa caggaagcga cgacctaccg atacccatga gacgggcgag
      361 gcgccgatga ttctgcagac gcagcaggac cggagccggc gtatccgccg gctggagaga
      421 gcggacgtga cgcgatgagg gatcgacgag cgacgaagcg agttcggcgg gggatcgccg
      481 gtggagaacg tggcgctgcc gcgatcgagt tcgcactggt ggcgccgctg ctgttcctgc
      541 tggtcttcgg catcatcgac ttcggcttcg ggttccatgc gtgggacgcc tcccacaacg
      601 ccgcccgcga gggcgcgcgc gtgggcgccg tgaaccccaa cccgacgttg atcgaggccc
      661 gggcccgtgc cgcgtccgac ttcctcgacc agagcctcat cgacgtgacc gtgcaatgcg
      721 ccgccgaggg ctcgacgtcg ttcggcgcct gtggcgacgg ttccggatgg gtcgagggcg
      781 acgtggtccg cgtgaccgtg atctacgact acaactacat gacgccgctt cccaacatcg
      841 tcggcctcgg cgattccctc gccgtgaagg gcatctcaga gtcccgattc gagggtcagt
      901 gatggcgaag gcgcggcagc ccggccggat ccgcggcctg atcaaggatc gactgcacga
      961 cgagagcggg gccgtggtga tcatctcggc gctgctgatc gtcgtcctga tggggtcggt
     1021 ggccttcgcg gtcgacctgg cccgcctgcg ccacgagcgt caggtgctgc agacagcggt
     1081 cgacctcggc gcgctcgcgg gagccggcaa gcttccggcg cagggcggcg acgccgcggc
     1141 cgacgccgtg gccaccgcga cccgcatcgc gatcgcgaac gcgccccagc tcgcgagcgc
     1201 gacgctcgac atctcgttcc ggtgcgcggt cgccgacccc gagggtaacg gcggcgccga
     1261 cagccaggac gtcaagttcg cttgcggtcc cgacggcggc ggcgggtgga tcgatggttg
     1321 gaacacccgg cgggggcgcg cgttccactc gtgcgacccg tgggcgggcg acctgtgcaa
     1381 cacgatcgtg gtgcgcgcgt cgaacctggt gccctacttc ttcgctccgg tgctcggcat
     1441 cgaccaaggc tcgaccggca cggtgagcgg cgcgtcgtgc aagggcttct gcgggcagcc
     1501 ctcgagcccg ctcgacgtgg ccatggtgct ggaccgctcg acgagcatga cgaatgccga
     1561 catggccaac ctgaagaacg ctgcgctctc cgtgctcgac ttctacgatc cggcgctgca
     1621 gcacgtggcc ctcgtcgcgt tgccctatac caactcgacc aacaggtgca tcgccaaccc
     1681 gacccagaac taccctgccg tcgggcggat gtggcaggtc acgggcctgt cgagcgacta
     1741 caagagcggc ggctcgctca acaccagcag cgagctggtc acgaggatca actgcctcca
     1801 gcgagcgacg aacctcacca gcgttcgacc gagcggtggc cacaccgacc tcggcgatcc
     1861 gatggcgacg gcgcacgaca tcgtcgtcaa cgacgggcga ccggacgtcc ccgacgtgat
     1921 catcttcatg accgatggcg aggcgaacca acccaggggc ttgcagccgt gcgactactt
     1981 cgtcgatcgg gccatccctg cccaagacga cgaggtgaac gtgtacgtca tcgcctacgg
     2041 cgtggcgtcg gccaggtgcg gggaagacac caccggcccg tacaggggcg attacgcctc
     2101 gacgtcggct gccgacgcgg cgagcgggga ctcggatgac aacgcgccgg gtgggtgcgc
     2161 atccgacgag aacaccgacg gcgactacta cttctgcgag agcggcagca gcgacctcga
     2221 gccggtcttc cgccagatcg ccgtcgccgc gctggagcgg tcgaggctca tcgacttctg
     2281 acgggttccg ggtcccacac cacccgtctg cccggcgtta gcgtcctcca ccgatgagcg
     2341 atgcgtggat ccgcgccctc gtggcgctgc cgttcgggct cgcgatcggg agcttcatga
     2401 cggtcgtcgt ctcgcgtgtg ccggcggggg agagcgtgct gcggccccgc tcgcgctgca
     2461 cgagctgcgg caccgagatc cgcaaccgag acaatgtgcc ggtgttgtcg tgggtgctcc
     2521 ttcggggacg gtgccgatcg tgcggtgagc gcatctcggc gcgctacccc ctgctcgagc
     2581 tctccaccgc ctcgctcgtg gtggccgcgg ccgcggccta cgagcagatc tggctcgcgg
     2641 ccatgatcgc ggcattcctg tcgttgatgc ccgccgtcgc ctggatcgac atcgagcatc
     2701 ggatcatccc gaaccgcatc acctacccgg ccttcctggg cttcagcgcc tacgtcgtgg
     2761 tcgcgtggct cttcgacggc gggaccgatc cggtccgcgc gctggcgggc gccctgatct
     2821 acggcggtct cctgttcgtg gtcgccctcg tctctcgggg catgggcatg ggcgacgtga
     2881 agctggcact cgtgatcggc gtcgtgctcg gtgcggtcgg tctgcgctac gtcggggtgg
     2941 cagccgccgg agccgtgctc ttcggaggga tcggcggcat cgtggcgctg atcgtcggtc
     3001 ggaaccgcaa ggcgatgatc cccttcgggc cgtacatggc ggcgggcgcg gtggtcgggg
     3061 cgttctgggg cgagccgatc gccgagtggt acctgcggtc gttcagcgca acggccctct
     3121 gagggcagcc tccggcgctc gaacacacgt tctccacaac gttccggcag atgtttgact
     3181 tgccctgtgc cctgatgttt cgtgagcatt ctcaacagca ctcctgtgtc tcgatgtggc
     3241 tggtgtgaac tgatgtgact gatgtgacgg aaggaagcgg catggccgac gcgcaaccgt
     3301 tcgtgaccgg agcgctgctc acggtcgcgg aggtcgccgc cggcatgcgc gtgtcgaaca
     3361 tgacggtcta ccgattgatc aagagcggcg agctgcctgc cgtccgggtg ggcaagggct
     3421 accgcatccg cgagtcggag ctgcagcgct tcctcgagga gcgttccgtc caggtggagg
     3481 ggggctagtg gccaaggggc gcatcggcct cgatatcggc tcgaccgcgg tgcgggccgt
     3541 cgagctcacg gggggcgatc cgcccaccgt gatcaaggcc gcccaggtgc ccttgcccgc
     3601 cggcgcggtc gagaacgggg aggtcaagga ccccgacgtc gtgaccgagg ccctgcgcga
     3661 actctgggag cgcggtgggt tcaaggccaa gcgcgtctac atgggtgtcg gcaaccaacg
     3721 ggtcgtggtg cgtgagatcg ccctgcccta cctgcccgaa aaggagctca gggcgtcgct
     3781 cgggttccag gtgcaggagt tcatcccgat ggccgtcgac gaggccgtgc tcgactacga
     3841 cccgatcggg gaattcgagc aggacgatcg caagatgttg cgcatgctgc tggtcgcggc
     3901 acagcgacag atggtcgaca ccgtggtgtc cgcagcgacc ggggccaagc tcgagccggt
     3961 cggcctcgac ctcgttccct tcgcgatggt tcgttcggtc ggcacgaccg gcgtcggcat
     4021 ggatctcgag gacgagggtg acgaggcggt catcgacatc ggcgcccacg tcaccaacat
     4081 cgtggtgcac gcgcgaggca ccgttcgctt cgtccgcatc ctcccgtcgg gggggcgcga
     4141 cgtgacgttg gcgatcgctc gcggcatcgg cgtggaggac gacgtcgccg aacggctgaa
     4201 gcggggtgag gacgtcgagc tcgagacgcc gccccgcgtc gaccaggagg gtggcaacga
     4261 ggtcgcggag cctcccgaca cgggccgggt caaggagatc gcgatgcaga gggcggcgag
     4321 cttcgtcgac gagatccgct cgtcgctgga gttctacacc gcccaggcgc agggggctcg
     4381 catcgcacgc gtgctggtca ccggcggcgg ctcgaagctg gaggggttcc tcgagctgct
     4441 ccgtcagcgc atccccgtgg aggtcgaggc gggccaggtg ttccagcacg cccgctcaca
     4501 gctctcgctc tccgatgaag cgctcgcgga ggctgagccc gtcctcgccg tcgccgtcgg
     4561 gctcgcgatc ccggggagga acgcgtgagc caggtcaacc tcctcccccc cgatatcctg
     4621 caggcacagc ggtatcggcg cctgacctcc ggggtggtcg tcgcgggcgt cgtcctggtc
     4681 gccgcggtct tcggctacta cctcttgcag agcaacgagc tgagcagtgt gaacgatgag
     4741 atcgcggccc aggaggccac gaacgcctcg atccaaggga gcatcgccga gaagcagaag
     4801 tacgccgacc tgcaggcgga ggcgcaggcg cagcaacagc tcctgagcgc ggcgtacgcg
     4861 ggcgaggtct cgttctcggc gctgttgatg gacttctcgc gtgtgatccc gtccgacgcc
     4921 tacgtcaaca gcctcgcgat ccaagccgac caggcggcgg gcggcaccga gggagcgacc
     4981 ccgggcctga tcggtgggat cacgggcagt ggtcaggcgg tcagcatcga cacgttgtcg
     5041 gtcttcctca cccgtctcga gcaggtgaag ggttgggtca acccgtggat gtccacggtg
     5101 agcaagaacg aggag
//
LOCUS       DATDBZ010000228         6534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2732988, whole genome shotgun sequence.
ACCESSION   DATDBZ010000228 DATDBZ010000000
VERSION     DATDBZ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732988"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..2265
                     /gene="uvrB"
                     /locus_tag="VIX62_05625"
     CDS             235..2265
                     /gene="uvrB"
                     /locus_tag="VIX62_05625"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY5859745.1"
                     /translation="MPPFEIKSEFRPAGDQPVAIEQLVSAVKGGDPHTTLLGATGTGK
                     TFTIAHVVEALQRPTLVIAPNKSLGAQLANEFREVFPDNAVEYFVSYYDYYQPEAYVP
                     STDTYIEKDSSVNEEIERLRHSATAALLGRRDVIIVASVSCIYGLGSPEEYEGQILRP
                     YRGTDIPLEFAMQRLVDIQYQRNNMSRARGTFRVQGDTLEVFPPYDTTGYRIEWWGDT
                     IEKISKFDPVTGELIKDDIIDLTVFPASHYVAGEERMKRAVVSIEDELEERLALLEGQ
                     GKLLEAERLRMRTRYDLEMMREVGFCSGIENYSRHIDGRTAGTAPYTLLDYFPDDYLV
                     VLDESHVTVPQLGAMYEGDLSRKSTLVEHGFRLPSAIDNRPLKFEEFTERVHQTVYMS
                     ATPSSYEIRHSRIVVEQIVRPTGLVDPEVQIRPTKGQVDDLIGEIRTRAEHEQRVLVT
                     TLTKKMAEDLTDYLVEMGIRVRYLHSEVDTLQRIEIVRDLRLGEFDVLVGINLLREGL
                     DIPEVSLVAILDADKEGFLRSETSLVQTIGRAARNVDGTVLMYADAITDSMKRAISET
                     QRRRQIQLRYNEEHGIDPTTIRRKITDILQQLRGGDDPAATHPKKERRRKAASHAEMP
                     TEELERLIESLEGEMHDAAKDLRFEYAARLRDEVHDLRKELRAMREAGVGAG"
     gene            2276..3313
                     /locus_tag="VIX62_05630"
     CDS             2276..3313
                     /locus_tag="VIX62_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5859746.1"
                     /translation="MTLVVRTPLAWLGPGRITPDVQITCEGDTITEVGPVRPVPDEAR
                     VITIEGFCMPAGADRHVHAELGDTVVMLRRGVTAVRDLAWPPDRIFPLAEASELPGFH
                     GPLIRAVGPMLTAPGGYPTKATWAPAGTGLEVADAGEAAGAVAALGQRGATAIKVSLN
                     AQAGPTPSDAVLMAVCDAAHGLELPVTAHAEGPGQVARALGAGIDELAHTPWTERLSD
                     DVIAAAAARMRWISTLDIHGFGRDSEALRIATDNLARFHRAGGSVHYGTDLGNGAIPS
                     GIHTRELRLMHEAGMAIEQVLASLVRAPLEPGAPADLLMLGDSALGDLGALDDIRLVV
                     KGGAVVAGTDH"
     gene            complement(3325..3732)
                     /locus_tag="VIX62_05635"
     CDS             complement(3325..3732)
                     /locus_tag="VIX62_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859747.1"
                     /translation="MAIRRFPFEFEPRYRRILWLLSVTPARSGVEIGDGSLRVRFGPW
                     RVQTDVSNIRCAEETGPYQALRAIGPHISMADQGLSFGSTTRGGVCLLFHEPVPGRET
                     LGLVKHPGLTVTVEDPVGLIDAIADEQRPADPG"
     gene            complement(3794..4915)
                     /locus_tag="VIX62_05640"
     CDS             complement(3794..4915)
                     /locus_tag="VIX62_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5859748.1"
                     /translation="MLSRKRYLISASLGPVSTRSREGLDRYMDAWGSKGAPDHVWMED
                     IFPQMALLKRTFAALTGCDTDELAITTNISIALATVASALDLRGERNRVILSELDFPT
                     DGHVWLAWAAKTGAEIVWLRSADGLTIPLEAYDEAIDERTAVVMVNRVLYRSSAIVDA
                     KAVCAMARERGALSFVDDYHGIGIVPLDLHDLGCDLYTAGTLKWLCGGPGLAFLYARR
                     ELLPTLEPAVTGWFATEEPFSFDNQHLDYHPTARRLEHGTPPAPVFFIAQGGLDIISE
                     VGVDRVRARQGVLTDHVIARSDEAGLVVRTPRARADRGGVVNVQVGPEAERICHALLA
                     QDICTDFRGDGIRISPHFFTTEDEIDACFDALGPILAGS"
     gene            complement(4950..5249)
                     /locus_tag="VIX62_05645"
     CDS             complement(4950..5249)
                     /locus_tag="VIX62_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859749.1"
                     /translation="MTTREALEAMPIDQLHDRTMKVARHRLDVGFLWDLVKALPVAEE
                     VAGDDQRSKVDIMRPLALLNDLVRDTDEGALGEALRPMYVDYLLEHDADWDGSDD"
     gene            complement(5261..>6534)
                     /locus_tag="VIX62_05650"
     CDS             complement(5261..>6534)
                     /locus_tag="VIX62_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5859750.1"
                     /translation="VAIDPTTGADRVLTSSDVQLGWAQGSPDGREVAVIEAVCSDRYV
                     VAGQLLLVDPASGARRPIDTAGVDVSWSEWRGPGRLFAIGLRGLDTVALEVDTASGDV
                     RETWVWAEGCGDFSPFASPIGTGDAFAAVVGGATRPTTLTRVEAGADHPLATIAHAGN
                     ERMWSAIAARERVTWTAPDGLKIEGFITLPATGTAPYPTIAQVHGGPISATTDHPVGA
                     WSGALLDRGYAIFQPNPRGSSGRGREFAARVIGDMGGGDARDILSGLDHLVERGLADP
                     DRIGVMGGSYGGFMAAWLPTVDGRFQAAVSASPVTDWYSERFDSTLGSWAADYVGGEP
                     LDVPEAYARQSPVLAKDLTTPTLLTAGLHDRATPVGQATEFYRALRGRGVPAEVVRYP
                     QEGHGVRNFPALIDFVARSVGWFERFMPPGR"
BASE COUNT         1068 a         2298 c         2228 g          940 t
ORIGIN      
        1 aactcgatcg gctggccttg ggcagtggtg ccgacgtagg tcgggtcctg gagggccgac
       61 gcagggaacg cgatggcgag caggaaggtc gcgagcccga aggcgatgga tcggtggacg
      121 ttcttcaaga ggggctgctc ctcgttcggg gaagcgagcc tgccacgggg gagcgttccc
      181 gcgcaacggc cgaacggacc caggcctgtc cgaagggggc catagactag gagcgtgccc
      241 ccgttcgaga tcaagtcaga gttccgtcct gccggtgacc aaccggtggc catcgagcag
      301 ctcgtgtcgg ccgtcaaggg cggcgacccg cacaccacgt tgctgggcgc caccggcacg
      361 ggcaagacgt tcacgatcgc gcacgtcgtg gaggcgttgc agcggcccac cctggtgatc
      421 gccccgaaca agtcattggg cgcgcagttg gccaacgagt tccgcgaggt gttcccggac
      481 aacgcggtcg aatacttcgt cagctactac gactactacc aacccgaggc ctacgtcccg
      541 tccaccgaca cctacatcga gaaggacagt tcggtgaacg aggagatcga gcggctgcgc
      601 cactcggcca ccgccgccct cctcgggcgg cgcgacgtga tcatcgtcgc cagcgtctcg
      661 tgcatctacg gcctgggctc gcccgaggag tacgagggcc agatcctgcg gccttaccga
      721 ggcaccgaca tcccgctcga gttcgcgatg cagcgactgg tcgacatcca gtaccagcgc
      781 aacaacatga gccgcgcgcg tggcacgttc cgtgtccagg gcgacaccct cgaggtcttc
      841 ccgccgtacg acaccacggg ctaccggatc gagtggtggg gcgacacgat cgagaagatc
      901 tcgaagttcg acccggtcac cggcgagctg atcaaggacg acatcatcga cctcacggtg
      961 ttcccggcct cgcattacgt ggccggggaa gaacggatga agcgcgccgt cgtctcgatc
     1021 gaggacgagt tggaggagcg cctcgccttg ctcgaaggtc agggcaagct cctcgaggcc
     1081 gaacggctcc gcatgcgcac ccgctacgac ctggagatga tgcgcgaggt cgggttctgc
     1141 agcgggatcg agaactactc gcggcacatc gacgggcgta ccgccggcac cgcgccgtac
     1201 acgctgctcg actacttccc cgacgactac ctggtggtcc tcgacgagag ccacgtcacc
     1261 gtgccgcagc tgggtgccat gtacgaaggc gatctgtcgc gcaagtccac cctggtcgaa
     1321 cacggcttcc gtctgcccag cgccatcgac aaccgcccgc tgaagttcga ggagttcacc
     1381 gagcgcgtgc accagaccgt gtacatgtcg gcgacgccaa gctcctacga gatccgtcac
     1441 tcgaggatcg tcgtcgagca gatcgtgcga cccaccggcc tggtcgaccc cgaggtgcag
     1501 atccggccca cgaagggaca ggtcgacgac ctgatcgggg agatccgaac gcgagccgag
     1561 cacgagcaac gcgtcctggt caccaccctc acgaagaaga tggccgaaga cctcaccgac
     1621 tacctggtcg agatggggat ccgggtccgc tacctgcact ccgaggtcga caccctgcag
     1681 cgcatcgaga tcgtgcgcga cctgcggctc ggtgagttcg acgtcctggt cggtatcaac
     1741 ctgctccgtg aagggctgga catccccgag gtgtcgctgg tggccatcct cgacgccgac
     1801 aaggaaggct tcctgcggtc ggagacctca ctggtccaga cgatcgggcg agccgcgcgc
     1861 aacgtcgacg gcacggtgct gatgtacgcc gacgccatca ccgattccat gaagcgcgcc
     1921 atcagcgaga cccagcgccg ccgccagatc cagctccgat acaacgagga gcacggcatc
     1981 gaccccacca ccatccgccg caagatcacc gacatcctgc agcagctccg cggcggcgac
     2041 gatccggccg ccacccatcc caagaaggag cgccgtcgga aggccgcgtc ccacgccgag
     2101 atgcccaccg aagagctgga gcgcctgatc gagagcctcg aaggcgagat gcacgatgcc
     2161 gcgaaggacc tccggttcga gtacgccgcg cggctccgcg acgaggtgca cgacctgcgc
     2221 aaggaactcc gagccatgcg agaggcgggc gtcggcgccg ggtagtgtcg gcgccatgac
     2281 cctggtcgtc cgcacgccgc tcgcctggct cggtccgggc cgcatcacgc ccgacgtcca
     2341 gatcacctgc gagggcgaca ccatcaccga ggtcgggccg gtgcgtccgg tgccggacga
     2401 agcgcgggtg atcacgatcg aggggttctg catgccggcg ggcgccgacc gccacgtgca
     2461 cgccgagctc ggcgacaccg tggtgatgct gcggcggggt gtgacggccg tgcgcgacct
     2521 cgcgtggcca cccgaccgca tcttcccgct cgcggaggcg tcggagctgc cggggttcca
     2581 cggccccctg atccgggcgg tcgggcccat gctcaccgcg cccgggggct acccgacgaa
     2641 ggcgacatgg gcgcccgcgg gcacggggct cgaggtcgcc gatgccggcg aggcggccgg
     2701 tgcggtcgcg gcactggggc agcgcggcgc caccgcgatc aaggtctcgc tcaacgcaca
     2761 ggcgggaccc accccgagcg acgccgtgct gatggccgtg tgtgacgccg cgcacggcct
     2821 cgagctcccc gttacggctc acgcggaggg tcccggccag gtggcgcggg cgctgggcgc
     2881 gggcatcgat gaactcgccc acacgccgtg gaccgagcgt ctctccgacg acgtgatcgc
     2941 ggcggccgct gcgaggatgc gatggatctc gacgctcgac atccacgggt tcggccgcga
     3001 cagcgaagcg ctgcgcatcg ccacggacaa cctggcgcgg ttccatcgag ccggcgggtc
     3061 cgtccactac ggcacggatc tcgggaacgg tgcgattccc tccgggatcc acactcgcga
     3121 actccgactc atgcacgaag ccggcatggc tatcgagcag gtgctcgcgt cgttggttcg
     3181 agctcccctc gaaccgggtg cgcccgccga cctcctgatg ctgggagaca gcgccctggg
     3241 cgacctgggc gccctcgacg acatccgtct cgtggtgaag ggcggagcgg tcgtcgcggg
     3301 caccgaccac tagtctgcgg tggactaccc cgggtcggcg ggccgttgct cgtcggcgat
     3361 cgcgtcgatg agcccgacgg gatcctcgac cgtgacggtg agaccgggat gcttcaccag
     3421 cccaagcgtc tcccggcccg gcacgggttc gtggaacagc agacacacgc cgccccgggt
     3481 cgtcgagccg aacgacaacc cctggtcggc catcgagatg tgcggaccga tcgcccgaag
     3541 cgcctgatac ggcccggtct cctccgcgca ccggatgttg ctcacgtcgg tctgcacgcg
     3601 ccacgggccg aaccgcaccc gcaacgatcc gtcaccgatc tcgacccccg aacgagccgg
     3661 ggtgacgctc aacagccaca ggatgcgccg gtagcggggt tcgaactcga acgggaaccg
     3721 tcggatggcc acgacgtcca atgtacgcgc gaggtgcgtg gagcccggcc acgagcctga
     3781 agtgcggagg ttcctacgac cccgccagga tcggcccgag cgcgtcgaag cacgcgtcga
     3841 tctcgtcctc ggtggtgaag aagtgcgggc tgatccggat cccgtcaccg cggaagtcgg
     3901 tgcagatgtc ctgggccagc agcgcatggc agatgcgttc cgcctcggga cccacctgca
     3961 cgttgaccac gccgccccgg tcggcacgcg ctcgcggcgt ccgcacgacc aacccggcct
     4021 cgtcgctccg cgcgatgacg tggtcggtca gcacaccctg ccgcgcgcgg acccgatcca
     4081 cgcccacctc gctgatgatg tcgaggcctc cctgcgcgat gaagaacacc ggcgccggcg
     4141 gggtgccgtg ctccaaccgc ctggcggtgg ggtggtagtc caggtgctgg ttgtcgaacg
     4201 agaagggttc ctcggtggcg aaccacccgg tcaccgccgg ctccagggtg ggcagcagtt
     4261 cccggcgtgc gtagaggaac gccaggccgg gtccgccgca cagccacttg agcgtgcccg
     4321 ccgtgtagag atcacacccc aggtcgtgca ggtccagcgg cacgatcccg atcccgtggt
     4381 agtcgtcgac gaacgacagg gccccgcgtt cccgcgccat cgcgcagacc gccttggcat
     4441 ccacgatcgc gctcgagcgg tagagcacgc ggttcaccat gacgacggcg gtgcgttcat
     4501 cgatcgcctc gtcgtaggcc tcgagcggga tcgtgaggcc gtcggccgat cgcagccaga
     4561 cgatctcggc gccggtcttc gccgcccagg ccagccacac gtgtccgtcg gtggggaagt
     4621 ccagctccga caggatcacc cggttccgtt cgccgcgcag gtcgagcgcc gaggccacgg
     4681 tcgccagcgc gatcgagatg ttcgtggtga tggccagttc gtcggtgtcg catcccgtga
     4741 gcgccgcgaa cgtgcgcttc agcaaggcca tctgcgggaa gatgtcctcc atccacacgt
     4801 ggtcgggggc gcctttggac ccccaggcat ccatgtagcg gtcgaggccc tcccgggaac
     4861 gggtcgacac cgggccgagc gaggcactga tcaggtagcg cttgcgggag agcaccggga
     4921 actcgtcccg gtaggagagg agttcgcgct cagtcgtcgg atccgtccca gtcggcgtcg
     4981 tgctcgagga gatagtccac gtacatcgga cgaagggctt cgcccaaggc accctcgtcg
     5041 gtgtcgcgca ccagatcgtt gagcagcgcg agcgggcgca tgatgtccac cttgctgcgc
     5101 tggtcgtcgc cggccacctc ctccgccacg gggagggcct tcaccaggtc ccacaggaac
     5161 cccacgtcga gccggtggcg ggccaccttc atggtccggt cgtgcagttg gtcgatcggc
     5221 atggcctcga gtgcttcgcg ggtcgtcatg gcgacagagc ctatcggcct ggaggcatga
     5281 accgttcgaa ccagccgacg gaccgggcca cgaagtcgat cagcgccggg aagttcctga
     5341 ccccgtggcc ctcctgcgga taccgcacca cctcggccgg cacgccgcgt ccgcgcagcg
     5401 cccggtagaa ctcggtggcc tgccccacgg gagtcgcgcg atcgtgcagg cccgcggtga
     5461 ggagggtggg cgtggtgagg tccttcgcga gcaccgggct ctgccttgcg taggcttcgg
     5521 gcacgtcgag cggttccccg ccgacgtagt cggcggccca cgatcccagc gtgctgtcga
     5581 atcgctcgga gtaccagtcg gtgaccggcg aggcgctgac ggccgcttgg aagcggccgt
     5641 ccaccgtggg cagccacgcc gccatgaacc ccccgtagct gccgcccatg acgccgatgc
     5701 ggtcggggtc ggcgagacca cgctcgacca ggtggtcgag tccactcagg atgtcccggg
     5761 cgtcgccgcc tcccatgtcg ccgatcaccc gcgccgcgaa ctcgcgaccg cgccccgacg
     5821 acccgcgagg attcggctgg aagatcgcgt acccgcgatc gaggagcgcg cccgaccagg
     5881 caccgaccgg atggtcggtg gtggcgctga tcgggccgcc gtggacctgg gcgatcgtgg
     5941 ggtagggagc ggtgcccgtc gccgggagcg tgatgaaccc ctcgatcttc aggccgtcgg
     6001 gcgccgtcca ggtcactcgc tcccgcgcgg cgatcgccga ccacatgcgc tcgttcccgg
     6061 cgtgggcgat cgtcgccaat gggtggtccg cgcccgcctc gaccctggtc aaggtcgtgg
     6121 gtcgcgtggc cccgccgacc acggccgcga acgcatcgcc ggtgccgatg ggcgaggcga
     6181 acggcgagaa gtccccgcag ccttcggccc atacccaggt ctcacgaacg tcgccggatg
     6241 cggtgtccac ctccaacgcc acggtgtcga gtccgcgcag tccgatcgcg aacagccggc
     6301 cgggccctcg ccattcgctc cagctgacat cgacgccggc ggtgtcgatc ggccgtcgcg
     6361 cgccggaggc cggatccacg aggaggagct ggcccgcgac gacgtaccgg tcgctgcaga
     6421 ccgcctcgat cacggcgact tcccggccgt cgggcgaccc ctgcgcccac cccagctgca
     6481 cgtcgctcga tgtcaggacc cggtcggccc cggtcgtggg gtcgatcgcg acca
//
LOCUS       DATDBZ010000229        11404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1507436, whole genome shotgun sequence.
ACCESSION   DATDBZ010000229 DATDBZ010000000
VERSION     DATDBZ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507436"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..501)
                     /locus_tag="VIX62_05655"
     CDS             complement(97..501)
                     /locus_tag="VIX62_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEY5859751.1"
                     /translation="MRSVEFQVDTSGTRVLDLTDRVEAFAAEAGEDGLVHVFIPHATA
                     GVALMETGSGSEADLEATLERLLPRDDRYQHRHGSVGHGGDHLLPVFVSPSLMIPAIG
                     GGLALGTWQRVVLVDPNRENDVRSVRLSFLAG"
     gene            complement(501..1235)
                     /locus_tag="VIX62_05660"
     CDS             complement(501..1235)
                     /locus_tag="VIX62_05660"
                     /inference="COORDINATES: protein motif:HMM:NF021079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HEY5859752.1"
                     /translation="MSRTSGIVGLIGALALIMGSFMAWAKLSFDGLPALTVKGIDRDG
                     KYSLACGVAVLVAAILVLVGKVAKRIPGILMLVFGALGAGVGILNVLTKDSQINDALD
                     QVSGDLTALGITSEEFKELFDVSWEIGIYIVIVGGLVALVAGILALKERSVEAVTQMP
                     MGGAGAGTAPPAAPAGTGFDAPPAPVSPAAPPPAAPDPVPTEPPSAPVTPSAPAAPQT
                     PPATPPAPPPASTEGDTPEDGGAPSR"
     gene            complement(1342..2346)
                     /locus_tag="VIX62_05665"
     CDS             complement(1342..2346)
                     /locus_tag="VIX62_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007118500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEY5859753.1"
                     /translation="MTMLPFTFRSLEDLDLGPVWAGVFETLWPHYREWFLRDGEAERP
                     SYATSVRMLRAHMPELLPAYERLVDLAGGGDLAARMLSLDKPPPYLAACSQGVWTREG
                     GPVLARNYDYAPSRFEGLIWSTDMVRPVIGTSDCLWGLLDGMNDAGLGVSLTFGGRRV
                     LGDGFGIPLVVRYLLETCETVAHARETLARLPYSLAHNLTVVDRDGEVLTAYLSPDRE
                     PIFRPFPAATNHQGIVEWPEQAKATRTIEREQCIVRVLGAEGQTAEGFADAFLRAPLF
                     STAYDSGWGTLYTAVYHPVDGVVDYRWPTHTWRLGFGSFTQAEHTEVLAEPTTADSAG
                     "
     gene            complement(2399..4015)
                     /locus_tag="VIX62_05670"
     CDS             complement(2399..4015)
                     /locus_tag="VIX62_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019297920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HEY5859754.1"
                     /translation="MRAIQVAAWLAALVLLIIVARLLVRRTHDASPPAADPAPTPMPA
                     GDRGSGANADRAPLVGIPAIRNFFRTNETPVYFVSATAFNLLGIDRWVRNFRFVNYYD
                     SFDGWHPNVFVPKEQSPRAFESIEEICNYLLGHKEVVDFIGGRGPGGKVAFLMFDQET
                     ETLARELDLQVAFPPAELRHRMDSKIETTRLGDDAGVPSVPNTMGRADTYREVLDLAG
                     AAGLGTDLVIQTPYGDSGQTTFFVASEDDWKQDEKKIVGHDLKVMTRIDPRECAIEGV
                     ITRHGTLVGPLMAELTGFPELTPYDGGWCGNDVFPDVLTEQQRLLARQRTFAMGERLR
                     AEGYRGYFELDFLADADSGEMYLGELNPRVTGASSITNVTAVAYGDMPLFLFHLLEFM
                     DVEYEIDVDELNQRWAHPGNVDEWTQFILKQTDEAVELITEAPSSGIWQMDGDRGIAF
                     SRRDTDWHTVGDGSEAFYLRIATAGGYRYPGADLGILVTRGRFLDDDHELVDRARDWI
                     AGIKGQFTSVPPPSQRTPEVQPEPFGFKML"
     gene            complement(3997..5403)
                     /locus_tag="VIX62_05675"
     CDS             complement(3997..5403)
                     /locus_tag="VIX62_05675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEY5859755.1"
                     /translation="MEPSPDETVTGAAYGGSRLTGDFESPGEYAFDGRGELATQPGAP
                     PEQAPEDSEILGAIEEQGDWMTEVLARLVRAETTLGNEEPGQALVRSILREDLHLEPV
                     DVPMDAEALRAHPHAAPFDWSLEGRSNVVAIWGPDEPDEISGRSLILNGHIDVVSPEP
                     LGQWGAHDPFGADRDGEWMIGRGAADMKCGLAAIVGAVRGLRSLGLAPRAPVTIESVL
                     EEECSGNGTLQTLLAGYTAEAAVIAEPFGAAITTSQVGVLWFSVRITGVPGHAAEGRN
                     ATNAIEKSLAVIGALRGLESDLNAAPPAPYDLFAHPINLNVGTIRGGDWPSTVPGECV
                     TGYRIALYPNMTLDDLKARIETLVAEATADQPGSAEVRYNGFASHGYELADDHDLVTT
                     LAGSFARQAGVPPALVATTGTTDASVFGNVAGIPAVCFGPFAEAAHGVGERVYLPSVI
                     QTAQVMGLFIRDWCGLSR"
     gene            5527..6069
                     /locus_tag="VIX62_05680"
     CDS             5527..6069
                     /locus_tag="VIX62_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859756.1"
                     /translation="MKNPRGHRHDHASLDEIQGWFAGRLPDGLYVGAVDVSTDRDEIL
                     VIGTIAEPDLAADASSEARAAAIASRIAGFREDTRGKRMQIADEAQHRFAKAVSWGAR
                     CADQQVLFTTLSVPAMTRLRMPERKVLDTLVEAGVARSRSHALAWCVRLVAERQDDWL
                     RDLQEALTKVEEVRAEGPRD"
     gene            complement(6079..6303)
                     /locus_tag="VIX62_05685"
     CDS             complement(6079..6303)
                     /locus_tag="VIX62_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859757.1"
                     /translation="MLGEVDALIVEVKPFDVHGVGYVDVTVAFRDRTVETARLGAESV
                     PADLRPGEQVVVTRAVNMVVSLRRADRDRA"
     gene            6469..7755
                     /locus_tag="VIX62_05690"
     CDS             6469..7755
                     /locus_tag="VIX62_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY5859758.1"
                     /translation="MNTLAIMATLGVSLLSGLLVLVPTANAVGTTPTHVDPTVGEAGT
                     GLPPLPVVVPKSTSAKAIALRWSDVGPRDAWARPAINFVGKDHDWMRDFAANGDGSYP
                     FRPRMLETRKYFARAVVKAFAPGAGVDPSITFPDLDPTQSFYKWANIAVQKGWMRPVS
                     DGRFLPDKPVTMVTVHRSIVLALGMRSTANQINHLRTRDGVAFATPMNVGTTNLGNRL
                     NLRYPSAEADHDVTPQSPMSRAQVAYSIYKAKTTAPGNVPWIRDQYEGIVLPNMGPTR
                     RAIVEWGLKYVGYPYVWAGEWGFDSPEPSALGGQPIAGFDCSGLMWWLMREDDGGYWE
                     ISPPRPHRGWPLPQRGSADMARFGNLRWDALLPGDLAFYDGSGDGTVDHVDLYIGNGY
                     ALDSSTSVGGVTLMYVGPGSWYRDHFVHGRRLLPTK"
     gene            complement(7759..8157)
                     /locus_tag="VIX62_05695"
     CDS             complement(7759..8157)
                     /locus_tag="VIX62_05695"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY5859759.1"
                     /translation="MHEVTITIRWRDVDNYGHVNNAVYLTYLEECRDRLVESLFGTEE
                     AWDFVLARVAIDYRNQLTQADGQVLVRCGVAGHGTSSVRTWERITKLDGTVAAESEAV
                     IVPRDPAGERSRPLTDEEQAILRAATEEPS"
     gene            complement(8339..9967)
                     /locus_tag="VIX62_05700"
     CDS             complement(8339..9967)
                     /locus_tag="VIX62_05700"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859760.1"
                     /translation="MFAAVLRRGALRRVLLAFFIFNAAEWAVWLAVLVYAFTIGGTTT
                     AGVVALLQLIPAALVAPLGSVVGDRLRRDHALALGYATQSAVMGATAIALWSNAPPWV
                     VFIGAIATSCSITLTRPVHNAILPELAETPEELTASNAASSTLEGLAIFVGPVLNGVL
                     IGIDGPWLVFAAMSAASVISALVTVHLPLREADGAEREDDPERLIADAREGFAQLRRE
                     PGALMLSLLVGAQFVVIGALDVLYTVTAIDVLDMGESGIGILASAVGVGGVVGAAATA
                     VLVGRRRMSPALGAGIIVTGIPLALVAFAQAPPFALVMLALSGAGKAFFDVAGRTLLQ
                     RTVRDEVLARIFGVQEGLLMAGLALGSIAAPVLVALFGARGAFVAVGAFLPLLGAISW
                     GRLRRIEEGGVVPGPELAVLLRIPFFQPLSQPVLERLSWNLVPVTAPAGSVVIREGDP
                     GDRFYMIEDGTVSVVAGGVPIATLGPGGYFGEIALLRNVPRTATITADSDLRLLALER
                     DDFLAAVTGSRPSSERADQEMDRRLGEHQPPSGR"
     gene            complement(10112..>11404)
                     /gene="groL"
                     /locus_tag="VIX62_05705"
     CDS             complement(10112..>11404)
                     /gene="groL"
                     /locus_tag="VIX62_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5859761.1"
                     /translation="PMSLKRGIERATELAVEAIKNQSKEVDDKAQIANVGAISAADPQ
                     IGETIAEAVDKVGKDGVITVEESNTFGMELDFVEGMRFDKGYISPYFVTDPERMEAVL
                     EDPYILIANSKVSAVKDLLPVLEKVMQAGKPVLILCEDVEGEALATLVVNKIRGTFRS
                     AAVKAPGFGDRRKAMLQDIAILTGATVISEEVGLKLESATIDLLGTARKVIVTKDETT
                     IVEGAGKDEDVQGRINQIKSEIENTDSDYDREKLQERLAKLSGGVAVIKVGAATEVEL
                     KEKKHRIEDAVQSTKAAVEEGIVPGGGVALLNAQAALDKVDLEGDELTGSNIVRRALE
                     EPIKQIAVNAGLEGGVIVEKVRALDPGFGLNAANGEYGDMLKFGVIDPTKVTRSALQN
                     AASIAALFLTTEAVVAEKPEKAPAGMPGGGMPDGGMDF"
BASE COUNT         1797 a         4011 c         3827 g         1769 t
ORIGIN      
        1 gaggacctga tcctgttgcg cgagcgcgac gtgcaggcgg tcacggccgc cgacgacaag
       61 gccgagcgtt ccccgggcca gtacctctag gccggctcag cccgccagga acgacagccg
      121 caccgaccgg acgtcgttct cccggttggg gtcgaccagt accacgcgtt gccaggtccc
      181 caacgcgagt cctccgccga tcgcggggat catcaggctc ggcgacacga acaccgggag
      241 caggtgatca ccgccgtggc caacgctccc gtgccgatgc tgatagcgat cgtcccgcgg
      301 caacaaccgc tcgagcgtcg cttccaggtc cgcctctgac cctgagccgg tctccatgag
      361 cgcgaccccc gctgtggcgt gcgggatgaa cacatgcacg agaccgtcct cgcccgcctc
      421 ggccgcgaag gcctcgaccc gatcggtgag gtcgaggacc cgggtccccg aggtatcgac
      481 ctggaactcg acgctgcgca ctatctggaa ggagcgccgc cgtcttcagg cgtgtcgccc
      541 tccgtgctgg cgggaggcgg ggccggcggc gtcgctggcg gcgtctgggg tgcggcaggc
      601 gccgacggtg tgaccggagc cgaaggcggc tcggtgggca ctggatcggg tgctgctggc
      661 gggggcgccg cgggcgagac cggcgccggc ggggcgtcga acccggtgcc cgccggagcc
      721 gcgggcgggg cggttcctgc gccggctccc cccatgggca tctgcgtcac agcttcgacc
      781 gagcgctcct tcagcgccag gatcccggcg accagcgcca ccaagccccc cacgatcacg
      841 atgtagatcc cgatctccca gctgacgtcg aacaactcct tgaactcctc ggacgtgatc
      901 cccaacgccg tgagatcacc cgagacctga tcgagagcgt cgttgatctg cgaatccttc
      961 gtgagcacgt tcaggatgcc caccccggcg cccaacgccc cgaacacgag catgaggatc
     1021 cccgggatcc gcttcgcgac cttgccgacg agcaccagga tggcggccac cagcaccgcg
     1081 accccgcatg cgaggctgta cttgccgtcg cggtcgatgc ccttcaccgt cagggcgggc
     1141 aggccgtcga aggacagctt cgcccacgcc atgaacgaac ccatgatcag cgcaagcgcg
     1201 ccaatcaacc ccacgatccc gctggtgcga ctcacgctgt gtgtcgttga tgctgccatc
     1261 gtcacggccc ccgtcgttcg gatgaaggca gtcttccacc ccggccgacg ggatgcgagc
     1321 ctcgtgactt ggtcgcccgg gctaacctgc cgaatcagcg gtggtcggtt cggcgaggac
     1381 ttcggtgtgc tcggcctggg tgaacgatcc gaacccgagc cgccacgtgt gggtgggcca
     1441 ccggtagtcg accaccccgt cgaccgggtg gtacacggcc gtgtagaggg tcccccaccc
     1501 gctgtcgtac gcggtgctga acaacggggc ccgcaggaac gcgtccgcga acccctcggc
     1561 ggtctgcccc tctgctccca acacacgcac gatgcactgc tcccgctcga tcgtgcgcgt
     1621 ggccttcgcc tgctccggcc actcgacgat cccctggtgg ttcgtggccg ccgggaacgg
     1681 ccggaagatc ggttcccggt cgggcgagag gtaggcggtg aggacctcgc cgtcacgatc
     1741 gacgacggtg aggttgtgcg cgaggctgta gggcaggcgc gccaacgtct cgcgcgcgtg
     1801 agccaccgtc tcgcacgttt ccagcaggta gcgcacgacg agcgggatgc cgaacccgtc
     1861 gccgagcacc cgccgccccc cgaaggtgag cgacacgccg agacccgcat cgttcatgcc
     1921 gtcgagcagc ccccacagac agtcgctggt cccgatcacg ggccgcacca tgtccgtgga
     1981 ccagatcagt ccctcgaacc gggagggcgc gtagtcgtag ttccgcgcca ggacggggcc
     2041 gccctcgcgc gtccagaccc cttgcgagca tgccgccagg tacggaggcg gcttgtcgag
     2101 ggagagcatg cgggccgcga gatcgccgcc gccggccagg tcgaccaacc gctcgtacgc
     2161 ggggagcagc tcgggcatgt gggcacgcag catgcgcacg ctggtggcgt aggaaggccg
     2221 ctccgcctcc ccgtcgcgca ggaaccactc gcggtagtgc ggccagagcg tctcgaacac
     2281 cccggcccac accgggccga ggtcgaggtc ttccagggag cggaacgtga acggcagcat
     2341 cgtcatcggg ggttgaggat acgggccgga tgggccccag ccggtatcgg caccaggttc
     2401 agagcatctt gaagccgaag ggctcgggct ggacctcagg cgtgcgctgg ctgggcggcg
     2461 gcacgctggt gaactgcccc ttgatcccgg cgatccagtc cctcgcgcga tccacgagtt
     2521 cgtggtcgtc gtccaggaac cgtccgcgcg tcaccaggat ccccaggtcg gccccggggt
     2581 atcggtaccc acccgcggtc gcgatgcgga ggtagaaggc ctcggatccg tcgccgacgg
     2641 tgtgccagtc ggtgtcgcgc ctggagaacg cgatgcctcg gtcgccgtcc atctgccaga
     2701 tccccgacga gggcgcttcc gtgatcagct ccaccgcctc gtcggtctgc ttgaggatga
     2761 actgcgtcca ctcgtcgacg ttgcccgggt gggcccagcg ctggttcagc tcgtcgacgt
     2821 cgatctcgta ctcgacgtcc atgaactcca ggagatggaa caggaacagc ggcatgtcgc
     2881 cgtaggccac cgccgtcacg ttcgtgatgc tggacgctcc cgtcacccgg gggttcagct
     2941 ctcccaggta catctcgccg gaatcggcat cggccaggaa gtcgagttcg aagtatccgc
     3001 ggtacccctc ggcgcgcagg cgctcgccca tcgcgaacgt gcgctggcgg gccagcagcc
     3061 gttgctgctc ggtgagcacg tcggggaaca cgtcattgcc gcaccagccg ccgtcgtagg
     3121 gggtcagctc cgggaacccg gtcagctccg ccatcagggg ccccaccaag gtcccgtgac
     3181 gggtgatcac cccctcgatc gcgcactcgc gcgggtcgat gcgggtcatc accttcaggt
     3241 catgtcccac gatcttcttc tcgtcctgct tccagtcatc ctcgctcgcg acgaagaacg
     3301 tcgtctggcc cgaatcgcca tagggcgtct ggatcacgag atcggtgccc aggccggcgg
     3361 cgcccgcgag gtcgagcacc tcgcggtagg tgtccgcccg ccccatcgtg ttgggcacgc
     3421 tggggacgcc ggcgtcatcg ccgagccgcg tggtctcgat cttggaatcc atccggtggc
     3481 ggagttcggc gggggggaac gccacctgga gatcgagttc gcgggccagg gtctcggtct
     3541 cctggtcgaa catcaggaac gccaccttgc cgccggggcc acggccaccg atgaagtcga
     3601 cgacctcctt gtgccccagc aggtagttgc agatctcctc gatggactcg aacgctcgcg
     3661 gggactgctc cttcggtacg aacacgttcg gatgccatcc gtcgaacgag tcgtagtagt
     3721 tgacgaaccg gaaattgcgc acccaccggt cgatccccag caggttgaac gcggtggcgc
     3781 tgacgaaata gaccggggtc tcgttcgtac ggaagaaatt gcggatcgcc gggatgccga
     3841 ccagtggtgc acgatcggcg ttggcgcccg agccacggtc accggcgggc atcggcgtcg
     3901 gggcggggtc ggcagccggg ggggaggcgt cgtgggttcg ccgcacgagc agccgtgcga
     3961 cgatgatcag caggacgagg gcggcgagcc aggctgctac ctggatagcc cgcaccagtc
     4021 ccggatgaac aagcccatga cctgcgccgt ctggatcacg gacggaaggt acacccgttc
     4081 ccctacgccg tgcgcggcct cggcgaaggg cccgaaacac acggccggga tgccggccac
     4141 gttgccgaac acggaggcgt cggtggtgcc ggtggtggcg accagcgccg gtggcacccc
     4201 ggcctgcctc gcgaacgacc ctgcgagcgt ggtcaccagg tcgtggtcat cggccagttc
     4261 gtagccgtgg ctcgcgaagc cgttgtagcg cacctcggcc gaccccggtt ggtcggcggt
     4321 cgcctcggcc accagcgttt cgatccgcgc tttgaggtcg tccaacgtca tgttcggata
     4381 cagcgcgatg cggtagccgg tgacgcactc gcccggcacg gtggagggcc agtcgccgcc
     4441 gcggatcgtg ccgacgttca gattgatcgg atgggcgaac aggtcgtagg gcgccggagg
     4501 ggcagcgttc agatcggact cgagacctcg gagcgcaccg atcaccgcga ggctcttctc
     4561 gatcgcattc gtggcgttgc ggccctcggc ggcatgtccc ggcacgcccg tgatccgcac
     4621 gctgaaccag agcaccccca cctgtgaggt ggtgatcgcg gcgccgaacg gctcggcgat
     4681 gacggcggcc tcggccgtgt agccggccag cagggtctgc agcgtcccgt tgccgctgca
     4741 ctcttcctcc agcaccgact cgatcgtgac cggcgcccgg ggggcgagtc ccagggaccg
     4801 gagaccccgc acggcgccca cgatggcggc cagaccgcac ttcatgtcgg ccgcgcccct
     4861 gccgatcatc cactccccgt cccgatcggc gccgaagggg tcgtgcgcgc cccactggcc
     4921 caatggctcc gggctgacca cgtcgatgtg tccgttgagg atcagcgaac gacccgagat
     4981 ctcgtccggt tcgtcgggcc cccagatggc gacgacgttc gatcggcctt ccagcgacca
     5041 atcgaacggc gccgcatgtg gatgagctcg tagcgcctcg gcatccatcg gcacgtcgac
     5101 cggttcgagg tggaggtcct cgcgcaggat cgagcgcacc agggcctgcc ccggttcctc
     5161 gttgccgagc gtggtctcgg cgcgaacgag ccgggcgagt acctcggtca tccagtcgcc
     5221 ctgctcctcg atggcgccga ggatctccga gtcctccggt gcctgctccg gaggggctcc
     5281 gggctgcgtt gcgagctcgc cccgaccgtc gaaggcgtac tccccagggg attcgaagtc
     5341 accggtgagg cggctcccac cgtaggcggc gccggtcacc gtctcgtcgg gcgagggctc
     5401 catggtggcg cagcgtacac aggaccttgc ccccgatgct cggcgcgggc gtcggatcgg
     5461 cactctgttg acaagccagt tgacacatct gttgacagac cgatgtaatg atgtaatctt
     5521 cattgcatga agaacccacg aggacaccga cacgaccacg catcgctcga cgagatccag
     5581 ggatggttcg ctggcagact ccccgacggg ctgtacgtcg gtgcggtcga cgtgagcacc
     5641 gatcgggacg agatcctcgt catcggtacg atcgccgagc ccgacctcgc ggccgacgca
     5701 tcgtccgaag ccagggccgc cgcgatcgcg tcgcgcatcg ccggtttccg cgaggacacc
     5761 cgcggcaagc gcatgcagat cgccgacgag gcgcagcatc ggttcgccaa ggccgtgagc
     5821 tggggggccc gctgcgccga ccagcaggtg ctgttcacca cgttgagcgt gccggcgatg
     5881 acccggctac ggatgcccga acgcaaggtc ctcgacacgt tggtcgaagc cggcgttgcc
     5941 cgcagccgca gccacgcgct ggcctggtgc gtccggctgg tcgccgagcg tcaggacgac
     6001 tggctccgtg acctgcagga agccctcacg aaggtggagg aagtgcgggc cgaggggccg
     6061 cgcgactagc gcgcgccgct aggcacgatc gcggtcggct cggcggagcg agacgaccat
     6121 gttcacggct ctcgtgacga ccacctgctc cccgggccga agatcggccg ggacactttc
     6181 ggctcccagg cgggcggtct ccacggttcg gtctcggaag gcaacggtga cgtcgacgta
     6241 gcccactccg tgcacgtcga agggcttcac ttcgactatg agcgcatcca cctctccgag
     6301 caccggccga gcctacctca gggggcgtag ggaggctccc cctgcgcagc tgcaggacct
     6361 gctgctaggg tccgggcgat gcggccccac cgcgcccggc tcaaggactc acgccggatt
     6421 gccgattctc cctgcatgca tcgaggggga tctcggcttc gccgtcgcgt gaacacgctc
     6481 gccatcatgg cgacgctggg ggtctccctg ctctccggcc tgctcgtcct cgtccctacg
     6541 gcgaacgcgg tagggaccac acccacccac gtcgatccca ccgtcggtga ggcaggaacg
     6601 gggctcccac cgctgccggt ggtggtaccg aagagcacct cggccaaggc catcgccctc
     6661 cgctggagcg acgtcgggcc gcgcgacgcg tgggccaggc cggcgatcaa cttcgtgggc
     6721 aaggaccacg actggatgcg ggacttcgcc gccaacggcg acggctccta ccccttccgg
     6781 ccgcggatgc tggaaacccg caagtacttc gctcgggccg tggtgaaagc gttcgcgccg
     6841 ggcgccggcg tcgatccctc gatcacgttc cccgacctgg acccgaccca gtcgttctac
     6901 aagtgggcga acatcgcggt gcagaagggg tggatgcgtc cggtcagcga cgggcgcttc
     6961 ctgccggaca aaccggtgac catggtcacc gtccaccgat cgatcgtctt ggcgctgggg
     7021 atgcgctcga cggccaacca gatcaaccac ctccgaaccc gggacggcgt cgcgttcgcc
     7081 accccgatga acgtgggcac gacgaacctg ggcaatcgcc tcaaccttcg ataccccagc
     7141 gcggaagccg atcacgacgt gacaccgcag tcaccgatgt cgcgcgccca ggtcgcctat
     7201 tcgatctata aggccaagac gaccgccccc ggcaacgtcc cttggatccg cgatcaatac
     7261 gaggggatcg tcctgccgaa catgggtccc actcgccggg ccatcgtcga gtggggtctg
     7321 aagtacgtgg gctatcccta cgtctgggcc ggtgaatggg gcttcgactc gcctgagcct
     7381 tccgcgctcg gcggacaacc gatcgccggg ttcgactgct cgggcctgat gtggtggctg
     7441 atgcgtgagg acgacggcgg atactgggag atctcgccgc cccgccccca ccgggggtgg
     7501 ccgctgccgc agcgcggttc cgcggacatg gccaggttcg gcaacctgag atgggacgcc
     7561 ctgcttcccg gagacctcgc gttctacgac ggcagcggcg acggcaccgt ggaccatgtg
     7621 gatctgtaca tcgggaacgg ctacgcgctc gactcctcca ccagcgtggg cggcgtcacg
     7681 ctgatgtacg tcgggcccgg cagctggtac cgcgaccact tcgtccacgg gcgacgactg
     7741 ctcccgacga agtagcgctc agctcggttc ctcggtcgct gcccgcagga tcgcctgttc
     7801 ctcatccgtg agtggacggc tccgctcgcc ggcaggatcc ctgggcacga tcaccgcctc
     7861 ggactcggcg gccacggtgc cgtcgagctt cgtgatccgc tcccaggtcc gcacgctcga
     7921 ggtcccgtgc ccggcgaccc cgcatcgcac caggacctga ccatcggcct gcgtcagctg
     7981 gttccgatag tcgatcgcga cccgggccag cacgaagtcc caagcctcct cggtaccgaa
     8041 cagggactcc accagccggt cgcggcactc ttcgaggtag gtcaggtaga ccgcgttgtt
     8101 gacgtggccg tagttgtcga cgtcgcgcca gcggatcgtg atcgtcacct cgtgcacggc
     8161 gcgtatcctc ccacgccgcc ggccccgacc atggcccgga catgggaaaa ggccccggac
     8221 ggggggtccg gggccttctc gtcggagaga ggggctccac gggtagttcg gttcgatccc
     8281 ggaggtggtt cgcgccggga tgttgcggtt gtgttgcaga tcgccgaccg ggcccgtctc
     8341 accgcccgct gggcggctgg tgttcaccga gccgacggtc catctcctgg tcggcgcgct
     8401 ccgagctcgg ccgcgacccg gtgacggcgg ccaggaagtc atcgcgttcg agcgcgagga
     8461 ggcggaggtc cgagtcggcc gtgatcgtgg ccgtgcgagg cacgttgcgg agcagcgcga
     8521 tctctccgaa gtagccgccg ggtcccagcg tcgcgatcgg cacgccgccg gcaaccacgc
     8581 tcaccgtccc gtcctcgatc atgtagaagc gatcgccggg atcaccctcg cggatgacga
     8641 ccgatcccgc cggcgccgtg acgggtacga ggttccatga caagcgctcg agcaccggct
     8701 gcgagagcgg ctggaagaac gggatacgca gcagcaccgc gagctccggc ccgggcacca
     8761 cgccgccctc ctcgatgcgc cgcagccgtc cccacgagat cgcaccgagc aggggcagga
     8821 atgcgccgac ggcgacgaag gccccgcggg ccccgaagag cgccaccaac accggcgccg
     8881 cgatcgagcc gagggccaac ccggccatca gcagtccttc ctgcaccccg aagatccgcg
     8941 cgagtacctc gtcacgaacg gtccgctgca ggagcgtgcg tccggccacg tcgaagaacg
     9001 ccttgccggc accgctcagc gccagcatca ccaacgcgaa cggaggcgcc tgcgcgaagg
     9061 ccaccagtgc gagcgggatc cccgtgacga tgatcccggc gccgagcgcc gggctcatcc
     9121 gccggcgccc gaccaggacg gcggtcgcgg cggcacccac cactccgccg acccccacgg
     9181 ccgaggccag gatcccgatc ccggactcgc ccatgtccaa cacgtcgatc gcggtcaccg
     9241 tgtagagcac atcgagcgcg ccgatcacca cgaactgagc acccacgagc aggctcaaca
     9301 tcagggcgcc gggctcacgg cggagctggg cgaacccctc tcgggcgtcg gcgatcaacc
     9361 gctcgggatc gtcctcgcgt tcggccccgt cggcctcccg caacggcagg tgcaccgtga
     9421 cgagcgcgct gatcaccgag gcggcgctca tcgccgcgaa gacgagccat ggaccatcga
     9481 tcccgatcaa caccccgttg agcaccggcc cgacgaagat cgcgagtcct tccagggtgc
     9541 tggaggcggc gttggaagcc gtcagctctt cgggtgtttc cgccagctcc ggcaggatcg
     9601 cgttgtggac gggcctggtc agggtgatcg aacagctggt ggcgatcgcc ccgatgaaga
     9661 ccacccatgg cggggcgttc gaccacaacg cgatcgccgt cgcccccatg accgccgact
     9721 gggtggcgta gccgagcgcg agcgcgtgat ccctacggag ccggtccccc acgaccgatc
     9781 ccaacggagc gaccagcgcg gcggggatca gctggagcag cgcgacgacg ccggcggtcg
     9841 tcgtgccgcc gatcgtgaag gcgtacacga gcaccgcgag ccagaccgcc cattcggcgg
     9901 cgttgaagat gaagaacgcg agcagcacac ggcgcaacgc tccgcgccgg agcaccgcgg
     9961 cgaacacgcg agcggtatcc ctgccggcca gcgaggcctc ctcggatgca ctggggcccg
    10021 ctcatcctgg ggtgccgctt gtgaacggtc aaaacgaaga acgggggccc gaaggccccc
    10081 gttctcatgg tggtgcgatg gtgccgacga gctagaagtc catgccaccg tcgggcatgc
    10141 cgccaccggg catgccggca ggggccttct cgggcttctc ggcgacgacg gcttcggtcg
    10201 tcaggaacag ggcggcgatc gacgcagcgt tctgcaatgc cgagcgcgtc accttggtcg
    10261 ggtcgatcac gccgaacttc agcatgtcgc cgtactcgcc gttggcggcg ttgagcccga
    10321 acccgggatc cagcgcacgc accttctcga cgatgacgcc accctcgagg ccggcgttca
    10381 cggcgatctg cttgatcggc tcctcgagcg cacggcgcac gatgttggaa cccgtgagct
    10441 cgtccccttc gaggtcgacc ttgtcgagcg ctgcctgcgc gttcagcagg gccacgccac
    10501 cgccgggcac gatgccctct tcgacggccg ccttggtgga ctgcacggcg tcctcgatgc
    10561 ggtgcttctt ctccttgagc tcgacctcgg tggccgcgcc gaccttgatg accgccacgc
    10621 cgccggagag cttggccagc cgctcctgca gcttctcgcg atcgtagtcg gagtcggtgt
    10681 tctcgatctc gctcttgatc tggttgatgc ggccctggac gtcctcgtcc ttgcccgcgc
    10741 cttccacgat cgtggtctcg tccttcgtca cgatcacctt gcgggcggtg cccagcaggt
    10801 cgatcgtggc gctctcgagc ttgaggccga cctcctcgga gatcaccgtg gcaccggtca
    10861 ggatcgcgat gtcctgcaac atggccttgc ggcggtcacc gaacccggga gccttcaccg
    10921 cggcgctgcg gaacgtgcca cggatcttgt tgacgaccag ggtggccaac gcctcgccct
    10981 cgacgtcctc gcacaggatc aggacgggct tgccggcctg catgaccttc tcgagcaccg
    11041 gcagcaggtc cttcacggcc gagaccttcg agttggcgat caggatgtac gggtcctcga
    11101 gcacggcttc catgcgctcg gggtcggtga cgaagtacgg cgagatgtag cccttgtcga
    11161 accgcatgcc ctcgacgaag tcgagctcca tgccgaaggt gttggactcc tcgacggtga
    11221 tgacgccgtc cttgccgacc ttgtcgacgg cctcggcgat cgtctccccg atctgggggt
    11281 cggccgcgga gatcgcgccg acgttggcga tctgggcctt gtcgtcgacc tccttggact
    11341 ggttcttgat cgcctcgacg gcgagctcgg tcgcacgctc gatgccgcgc ttcaggctca
    11401 tcgg
//
LOCUS       DATDBZ010000230         2167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2929168, whole genome shotgun sequence.
ACCESSION   DATDBZ010000230 DATDBZ010000000
VERSION     DATDBZ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2929168"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VIX62_05710"
     CDS             <1..917
                     /locus_tag="VIX62_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859762.1"
                     /translation="GFAVAGVATSMGVRGTGVTAAAALAMPVVWDSAYVAYNDTPVAA
                     FSLLAVGVVIASPRASLGAVGVAAGLIAVATSIKPTGVAAVGVVALVILMRYLFDDSA
                     AQSVSDGPRDANRGPFSRVLVQWLILAVPGVLALAFWSVRQYLYTGNLVDPAVVAEPD
                     AFARTMLPNALEQVLAPLMPFVSGIIGAFEPWGGRTAVVIQLFLVPAIIYVIWRRGDV
                     LRRFLLMVVPGWAHWVVLGLAIVRTRFHVISWTMIIVGLRIAVEDFIDRHPRGRLWIE
                     IAWTVGILAGLADVSLEMFRHIRQLWAA"
     gene            complement(951..1904)
                     /locus_tag="VIX62_05715"
     CDS             complement(951..1904)
                     /locus_tag="VIX62_05715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014348468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5859763.1"
                     /translation="MGTQGHPQIAVIIPCHDEEAAVATVVHEVRSAVPDAEIYVYDNA
                     STDRTAEVAAGAGANVRREPVKGKGNVIRRAFADVDADIYVLIDGDDTYATKDLPAMI
                     AALQAGPYDHVLGVRSGDASAYRPGHALGNRAFNWLTGTVFGASVSDMLSGYRVLSRR
                     FVKSFPALSSGFEIETELTVHAMGLRAPQVEVPVGFKDRAAGSASKLHTVRDGVRILS
                     LILTLTRHERPVLFFGVIGFVFAVVGLVLGLPVIIEFFETGLVPRLPTAVLALGTVII
                     AFMSWMIGVILDGVLQSRRELKRLSYLRLSPPDLDPQADLR"
BASE COUNT          309 a          709 c          757 g          392 t
ORIGIN      
        1 tggggttcgc cgtcgcaggc gtggccacga gcatgggcgt gcgcggaacg ggcgtgacgg
       61 cggcggcggc cctcgcgatg ccggtggtct gggattctgc atatgtcgcg tacaacgaca
      121 ccccggtcgc ggcgttctca ctgctcgccg tcggcgtcgt gatcgcctcg ccgcgggcgt
      181 cgctcggcgc cgtcggcgtg gcggccggcc tgatcgccgt cgcgaccagc atcaagccga
      241 cgggcgttgc ggcggtcggt gtcgtcgcgc tcgtcatcct gatgcgctac ctgttcgatg
      301 actccgctgc gcagtcggtg agcgacggcc ctcgggacgc gaaccgcggc ccgttcagca
      361 gggtgctggt gcagtggttg atcctggcgg tcccgggagt cctggcgctc gcgttctgga
      421 gtgtgcgcca gtacctgtac accggcaatc tcgtcgaccc cgcggtcgtc gcagagcccg
      481 atgcgttcgc gcgcaccatg cttccgaacg cgctcgagca ggtgctggcg ccactcatgc
      541 ccttcgtgag cggcatcatc ggtgcgttcg agccctgggg aggccgcacc gcggtcgtga
      601 tccagctgtt cctggtcccc gcgatcatct acgtcatctg gcgcaggggc gacgtcctcc
      661 gccgattcct gctgatggtg gttcccgggt gggcgcactg ggtggtgctc ggcctggcga
      721 tcgtgcgcac gcgtttccat gtgatctcgt ggacgatgat catcgtgggc ctccgcatcg
      781 cggtcgagga cttcatcgac cgccacccac gggggcgact ctggatcgag atcgcctgga
      841 cggtgggcat cctggccggt ctggccgatg tctcgctcga gatgttccgg cacatccgac
      901 aactctgggc ggcgtgagcg cgcttcagtg cgacggccgg cgatctgtcg ttaccgaagg
      961 tcggcttgcg gatcgaggtc gggcggcgag agtcgcaggt agctcagtcg cttgagctcg
     1021 cggcgtgatt gcagcacgcc gtcgaggatc acgccgatca tccacgacat gaacgcgatg
     1081 atgacggttc ccagggcgag aaccgcggtc ggaagacgtg gcacgaggcc cgtctcgaag
     1141 aactcgatga tgaccgggag tccgagcacg agccccacga ccgcgaacac gaatccgatg
     1201 acgccgaaga agagcacggg tcgctcatgg cgggtgagcg tgaggatgag cgacaggatt
     1261 cgcaccccgt cgcgtacggt gtgcaatttg ctcgcgcttc cggctgcccg gtccttgaac
     1321 ccgacgggca cctcgacctg cggcgcccgc aaccccatcg cgtgcacggt caactcggtc
     1381 tcgatctcga agccgctgga aagtgccggg aagctcttga cgaacctgcg cgacaagacc
     1441 cggtagccgc tcagcatgtc gctgaccgaa gcgccgaaga cggtgccggt caaccagttg
     1501 aatgcgcgat tgccgagtgc gtgtccgggt cgatatgcgc tcgcatctcc ggaccgcaca
     1561 ccgagcacgt ggtcgtacgg gccggcttgg agcgccgcga tcatcgcggg gagatccttg
     1621 gtcgcgtagg tgtcatcgcc gtcgatgagc acgtagatgt ccgcgtcgac atcggcgaag
     1681 gcccgacgga tgacgttgcc cttgcccttg accggctcgc gtcgcacgtt cgcgcccgcg
     1741 cctgcggcga cctcggcggt gcggtccgtg ctggcgttgt cgtacacgta gatctcggcg
     1801 tcggggaccg cggagcgaac ctcgtgcacg acggtggcga cggccgcctc ttcatcgtgg
     1861 cacgggatga tgacggcgat ctgaggatgc ccctgcgtgc cgatgtcggc ctccgatgct
     1921 cggcgtgaga ccgcgatccg gccgcactca cggtcccatt atcattggac agatcgtcgc
     1981 gcgagcacca ccacccggaa gggggcagcc tgtgtccgac gagggccccc ggcatgtcgg
     2041 cgtgctccgg cgaggcggcg ccttcctcgt cgtcggtgcg ctcgccttcg tgttggacgc
     2101 ggccgtcttc aacctgctca cgttcggttt cacgggtcag ggcccgatgt acgcgtcgcc
     2161 gatctgg
//
LOCUS       DATDBZ010000231        12991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_283035, whole genome shotgun sequence.
ACCESSION   DATDBZ010000231 DATDBZ010000000
VERSION     DATDBZ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12991
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283035"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VIX62_05720"
     CDS             <1..251
                     /locus_tag="VIX62_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY5859764.1"
                     /translation="GAQMVAPTSVGTAPPVPMLGHVTSSYRSPTLGRTFALAMVAGGR
                     ERIGTTLVAPLQGGDIEAEVTAPVFYDERNERRDGGGA"
     gene            248..796
                     /locus_tag="VIX62_05725"
     CDS             248..796
                     /locus_tag="VIX62_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEY5859765.1"
                     /translation="MTTARDPLAGRVDDLARIGQVTDGGVVLEHVPFLAQVNLRLDPT
                     LAGRAPYPLPVDPNTAWEDGPRAALWLGPDEWVILGPPHAGREIVAELDAALDGSHRS
                     VVDVSANRVAVEISGPRAKEILSKGCSIDLHPSMWAPGMCAQTMLGKVQVVLHERADT
                     TGIAVRTSFANYLIDWFGATVG"
     gene            800..1369
                     /locus_tag="VIX62_05730"
     CDS             800..1369
                     /locus_tag="VIX62_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859766.1"
                     /translation="MGSEYLPLIRSLMELDAPIRIFGGFAEDALLGGRIRRHHDDVDV
                     MIARDALDRGLRMFEELGFAPPEIRFEPTAGSPLVMGTVREGLNLELSVIDIAEAGPP
                     SFTLPGEHGLVRVLLPPDTWSWPPCGLEGIVIRTISPLALYQIRSGLTIVGAMGPPRP
                     KDIASQQALRERFFPDEPEGSLEPVIELL"
     gene            complement(1383..2258)
                     /locus_tag="VIX62_05735"
     CDS             complement(1383..2258)
                     /locus_tag="VIX62_05735"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY5859767.1"
                     /translation="MAVRKRRSLGGLDLEQQAALRMLVAAPKFELIPLSNTAEKADEL
                     PHAATVTVTASPSHGIEATLALAEEVAGRGHVVIPHLSAHMIRDRAHLGEVLDRCREA
                     GFSRAFVVGGDAKDKGEFHDGLMLLRAIDEAGHPFTEIGVPSYPEGHVDLPDARLVEV
                     LLEKQRYASYMTTQMCFNPGAIVTWIGTMRAAGVALPVHLGTPGVAELTKLMTISARI
                     GIADSARYLKKNKRMVGSLLTPGKFGPDSLLEGMGAALTDPGAKLEALHLFTFNQVQA
                     TVDWQQRMLAALDEA"
     gene            complement(2271..3824)
                     /locus_tag="VIX62_05740"
     CDS             complement(2271..3824)
                     /locus_tag="VIX62_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HEY5859768.1"
                     /translation="MSDQAPEGRRGRQGGGRAARQAARLHAVAEKIPFITRTLQPFEV
                     LDEEGLSLVEHNADVILEQVGIEVHGAPDALELFKGAGADVDGERVRFPAGLCRQLVQ
                     ATAPREFTQHARNPEHSVQIGGNATVFAPAYGSPFVRDVDGGRRYGTIEDFRNFVKLA
                     NSSPFLHHSGGTVCEPVDLPVNKRHYDMVYAHMRYSDRPFMGSVTHPQRAEDTVSMAR
                     ILFGKDYLDDHCVVLSLINANSPLVWDSSMLGAARAYAEANQAVIMTPFILAGAMSPA
                     TVAGAATQTLAEALAGMAYVQLVRPGAPVVFGSFASSMSMQSGAPTFGTPEPALVLYT
                     LAACARRLGVPFRSGGSLTASKIPDAQAAYESANTLQPTVLGGVNFVLHAAGWLEGGL
                     TMGYEKFVMDLDQCGMMAAFAKGVDLSPNGQALDAIVENGPGQHFLGTAHTLANFESA
                     FFRSSIADNNSFEQWEIEGSADSITRANTIWKKMLAEYEAPVIDETVDEELRSWIEQK
                     KASFPDSNV"
     gene            complement(3838..5859)
                     /locus_tag="VIX62_05745"
     CDS             complement(3838..5859)
                     /locus_tag="VIX62_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007254395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEY5859769.1"
                     /translation="MSEAEGSLVVFTPSGKRGRFDEDTNLLDAARALGVDLDSVCGGR
                     GICGRCQVVVSEGEFAKHGITSKADHLTAFSEPESRYRERSGLADDRRLGCHARVCGD
                     LVVDVPPESQIHRQVVRKEADAHPIEVDPVVRLHYVEVREPDLADPSGDLQRLLEALE
                     RDWSLEWLACDPHVLATLQRTLRAGDWTATVAVHDGWTITAIWPGFHDVALGVAFDIG
                     STTVAGHLCDLHTGEVLASAGAMNPQIRFGEDLMSRVSYVMMNPGAETELTRVVRECV
                     DGLVAELCTGAGVDPNDVLDLTIVGNPIMHHLFLGLDPTELGGAPFALATDEAVRREA
                     RELDLTATNAGARVYVLPCIAGHVGADTAGVILSEAPHDQEIVNLLVDVGTNAEIVLG
                     NRDRMLAASSPTGPAFEGAQISCGQRAAPGAIERVRIDRETLEPRFRVIGQDTWSDDP
                     GFDAATVTGVCGSGIIEAVAELFLAGILTTDGVIDGGLGERSPRIQPDGRTFSYVLHD
                     VPGAPRLVITQNDIRQIQLAKAALYAGCRLLMDAFGIETVDRIRLAGAFGAHIDPTYA
                     LVLGLVPDCDPARVTSAGNAAGTGARIALLNRAARGEIEGVVRRVEKLETAVEPRFQE
                     HFVAAMGIPHTTEPFPRLAATIDLPAPRTSSGGTDRPRRRRRDAPDRTS"
     gene            complement(5856..6812)
                     /locus_tag="VIX62_05750"
     CDS             complement(5856..6812)
                     /locus_tag="VIX62_05750"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5859770.1"
                     /translation="MTDTVVSSATKEVVIGSGRPFVIIGERINPTGRKKLAEEMKAGD
                     FSTVIADTVNQVEAGVHMLDVNAGIPLADEPAILAATVKLVQSLTDVPLSIDSSIVAA
                     LEAGLEVYEGKPLVNSVTGEDERIEQVLPLVAKYGAAVVAITNDETGISEDPDVRFAV
                     AEKIVRAAADHGIAAADVVVDPLVMPIGALGGAGRQVFSLVRRLHDELGVNSTCGASN
                     VSFGLPDRNRLNGAFLAMAIANGLTSAITSPLHDEVREAVMAADVMMGNDQDCNAWIA
                     KHREQPAGGTGRRGGRAGRRGAGADGGPTLSVVPDPEATPEP"
     gene            complement(6809..7117)
                     /locus_tag="VIX62_05755"
     CDS             complement(6809..7117)
                     /locus_tag="VIX62_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HEY5859771.1"
                     /translation="MADLTVIWWRDIPVQVVAKQGRTSAKVPLSPRFQEAIDMAATRS
                     GLIGTDEYLNEWRRVTRECGDDLDAEASKEAHALEQAYGDSRLESLVRSGGTDEGEGS
                     "
     gene            complement(7128..7823)
                     /locus_tag="VIX62_05760"
     CDS             complement(7128..7823)
                     /locus_tag="VIX62_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HEY5859772.1"
                     /translation="MSDELDLSSLSDEDLTQQMHDDLYDGLADEITEGTNILLARGWG
                     ADRVLNEALVEGMRIVGIDFRDGILFVPEVLLAANAMKAGMAILRPLLAETGAERMGT
                     IVIGTVKGDIHDIGKNLVGMMLEGAGFDVFDLGINNPVEDYLAALEEHKPDILGMSAL
                     LTTTMPYMKVVIDEMVAKGIRDDYIVLVGGAPLNEEFGKAVGADAYCRDAAVAAETAQ
                     ALVMAKRAATANA"
     gene            complement(7843..10323)
                     /locus_tag="VIX62_05765"
     CDS             complement(7843..10323)
                     /locus_tag="VIX62_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859773.1"
                     /translation="MLRDRAQTVIIGAGIVGSSAAYHLSQMGVTDVLVIDQGPLFETG
                     GSTSHAPGIIFQTNGSRTMCRIAQDSVALYDTLEVDGERCWYGVGGIEVATTPERMEE
                     LKRRQGYARSYGIEGTELLSPMEAAERSPLLDPSTILGAYWVPSDGAGKGVLIATALA
                     RKAEAAGVVFEGGVTVTGFDIRAGRVHGVETDQGRIECDRVLLCAGFWGPSVGAMAGV
                     PVPLVAVQHQLVWTDPVPELAGLTGDTWASQPVVRHQDMSLYFRQRDDHYGVGNYRHE
                     PIVTPQSALRRHGDGPMPSLMPWTPDDFDLCEAETERMFPALAGRMRPSDPARSINGM
                     FSFTPDAGSVVGESGAVRGLWLCEAVWVTHAAGMAKQAVEWMVDGEPSYDLAEADANR
                     FYPFQASSPYVLERAKQQYREVYDIIHPLQQPSKPRNLRLTPFFARHQALGAECFTGA
                     GWERPQWFRANRDLVGGVTHEWSRRAGWAARGWSPEVGAEHLATRSNAALFDITPFAK
                     FDVEGDDALGYLERVFANRIDRPVGSIVYTAALTPKGGIRLDLTITRKDERLFRVVTG
                     GGSGQHDLAWLRAQVRDGEVVRITERTGGLFALGVWGPRARDILQAVTDADVSNDAFP
                     YMTARYLNVGEVNPVWAQRISYAGELGWELYGQIAMGDRVWDRLWEAGRGHGLIAAGG
                     GAFDSLRLEKGYRLWGQDINIEHDPLAAGLGFAVHWDKEFQGREALEAIRDAGGPAQR
                     LACMTFDDPGVVVMGKEPIWHDGGVVSYVTSAAYGYSIGQGIVYGYLPTALAAHGTAV
                     EVEYFGRRYAATVTADPLFDPKSERLKT"
     gene            complement(10406..11401)
                     /locus_tag="VIX62_05770"
     CDS             complement(10406..11401)
                     /locus_tag="VIX62_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY5859774.1"
                     /translation="MPAITVDDITTLDRIPRPEPEHVQRPVQTVTTAPSGFEGEGFPV
                     RRAFAGVDLALLDPFIHMDQMGEVEYAPGEPKGTPWHPHRGFETVTYIMDGTFEHQDS
                     NGGGGTITNGDTQWMTAGAGILHIEKPPEALVASGGLFHGIQLWVNLPKAEKWAAPRY
                     QDLRAGEVGLLASDDGGALVRVIAGSLASHTGPGDTHTPMTMVHATVSPGGQLDLPWD
                     PGYNALAYVLAGEGTVGVEARPVRTGQLAVFGAGDALRLQAATGQESRSPELEVLVLG
                     GRPIREPVAWYGPFVMNTKQELVQAYEDFQAGKLGSIPAVHNTPDEIVESSPGTD"
     gene            complement(11450..>12991)
                     /locus_tag="VIX62_05775"
     CDS             complement(11450..>12991)
                     /locus_tag="VIX62_05775"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5859775.1"
                     /translation="CPRRKQRTRTRFGDEGGPDGPPSVICGGDMSEHVKMFIGGAWVD
                     AEDGATFDATSPATGAVIGSVPEGSRADAQRAIDAANAAAPAWAGLSAFDRAAAMRRI
                     ATVIDERRDDLAHTLALDQGKPLVAEARDEVEELIAYFDMAAADATRMEGLMPPSVDA
                     NKRVLLYRVPRGVVSIISPWNWPYTKPGEILAPAIAYGNAVVWAPAPSTSVCAAAMAA
                     VLEAAELPPGVVNMVTGPGAVVGDEIAANPGTQAVGFIGSIATGETVASRAAGKDLLL
                     EMGGNGPLVILEDADVGAAVEATLAACFLNAGQSCTAGERILVHEAVREEFVTKLAAQ
                     VHDRIRLGDPFDDATTMGPVNNAATADKTERHVRDAVDHGAKVITGGDRVPKHGSDLF
                     FSATVLDGVTNDMEIAREETFGPVAPISTIRSEQEAIDLVNGWPYGLLSAVFTRDLGR
                     GLRYAEAVRAGWVNVNEGTNYWESHLPFGGRAGSHSGVGRVGGRFSMERLTELKTVVL
                     NWVEE"
BASE COUNT         1911 a         4659 c         4345 g         2076 t
ORIGIN      
        1 aaggcgcgca gatggtggcc cccacgtcgg tcggcacggc cccgccggtc cccatgcttg
       61 gccatgtgac gtcgagctac cgcagcccca cgctgggccg gacgttcgca ctggccatgg
      121 tcgcgggggg gcgtgaacgc atcggtacca ccctcgtggc gccgctgcag ggcggcgaca
      181 tcgaagccga ggtcaccgcg ccggtcttct acgacgagcg gaacgaacgc cgggacggag
      241 gaggagcgtg accaccgcgc gtgatccgct cgccgggcgc gttgacgacc tcgcccgcat
      301 cgggcaggtc accgacggcg gcgtggtgct cgaacacgtg ccgttcctcg cccaggtgaa
      361 cctgcggctg gaccccacgc tcgccgggcg tgccccctat ccgttgccgg tcgatccgaa
      421 caccgcctgg gaggacggcc cgagggcggc cctgtggctc ggccccgacg agtgggtgat
      481 cctgggaccg ccgcacgccg ggcgcgagat cgtcgccgag ctcgacgcgg cgctcgacgg
      541 atcgcatcgc tcggtcgtgg acgtcagcgc gaaccgcgtg gccgtggaga tctccggccc
      601 gcgggccaag gagatcctgt ccaaggggtg ctcgatcgat ctgcatccct cgatgtgggc
      661 acccggcatg tgcgcccaga cgatgctggg caaggtccag gtggtcctgc acgaacgcgc
      721 cgacaccacg ggcatcgccg tgcgcacctc gttcgcgaac tacctgatcg actggttcgg
      781 cgccacggtg gggtgagcga tgggcagcga gtacctcccg ttgatccgct ccctgatgga
      841 gctcgatgca cccatccgga tcttcggcgg cttcgccgag gacgcgctgc tcggcgggcg
      901 gatccggcgc caccacgacg acgtcgacgt catgatcgct cgcgacgccc tcgatcgggg
      961 actgcggatg ttcgaggagc tgggcttcgc gccgccagag atccgtttcg agcccacggc
     1021 gggttccccg ttggtgatgg gaaccgtgcg cgagggactg aacctggaac tcagcgtgat
     1081 cgacatcgcc gaggcgggcc cgccgtcgtt caccctgccc ggggaacacg gtctcgtgcg
     1141 ggtcctgttg ccccccgaca cgtggtcctg gccgccttgc ggactcgagg ggatcgtgat
     1201 tcggacgatc tcccccttgg cgctgtacca gatccgctcg gggctcacga tcgtcggagc
     1261 gatgggcccg ccgcggccga aggacatcgc gtcccagcag gcgctgcgtg agcgcttctt
     1321 cccggacgaa ccggaaggtt ccctggagcc ggtgatcgag ctcctctgaa gacgagcgcc
     1381 gatcaggctt cgtcgagcgc ggcgagcatg cgctgctgcc aatccacggt cgcctgcacc
     1441 tggttgaagg tgaacaggtg gagcgcctcg agcttggcgc cggggtcggt gagcgccgca
     1501 cccataccct cgagcagcga atcgggcccg aacttgcctg gggtgagcag cgaccccacc
     1561 atgcgcttgt tcttcttcag gtagcgggca ctgtcggcga tgccgatgcg cgcgctgatc
     1621 gtcatcagct tcgtgagttc ggccacgccc ggcgtcccca ggtgcacggg cagggcgacg
     1681 ccggccgcgc gcatcgtgcc gatccacgtg acgatcgcgc cggggttgaa gcacatctgc
     1741 gtggtcatgt acgaggcgta gcgctgcttc tccagcagca cctcgaccaa ccgggcatcg
     1801 gggaggtcga cgtgaccttc tggatagctc ggcacgccga tctcggtgaa ggggtggccc
     1861 gcttcgtcga tcgcccggag cagcatcagg ccgtcgtgga actccccctt gtccttggcg
     1921 tcgccgccca cgacgaacgc ccggctgaag ccggcctcgc gacatcgatc gagcacctcg
     1981 cccagatgcg cgcggtcgcg gatcatgtgc gccgacaggt gcgggatgac cacgtgccct
     2041 cggccggcaa cctcctcggc gagggcgagc gtggcctcga tcccgtgact gggtgaggcg
     2101 gtcaccgtga ccgtggcggc gtgcggcaac tcgtcggcct tctcggcggt gttcgagagc
     2161 gggatcagct cgaacttcgg cgctgccacg agcatgcgca gcgccgcctg ctgctcgaga
     2221 tccagccctc cgaggctccg tcgtttccgc accgccatgc cgctccctgc ctaaacgttc
     2281 gagtccggga aggacgcctt cttctgctcg atccagcttc tgagctcctc atcgacggtc
     2341 tcgtcgatca ctggagcctc gtattcggcc agcatcttct tccagatcgt gttcgccctg
     2401 gtaatcgagt ccgcggatcc ttcgatctcc cactgctcga aggagttgtt gtcggcgatc
     2461 gagctgcgga agaacgccga ctcgaagttg gccagcgtgt gcgccgtgcc taggaagtgc
     2521 tggcccggcc cgttctcgac gatcgcgtcc aacgcctgtc cgttcggtga gaggtccacg
     2581 cccttggcga aggccgccat catgccgcac tggtccaggt ccatgacgaa tttctcgtaa
     2641 cccatagtca ggccgccttc gagccagccc gctgcgtgca gcacgaagtt cacgccgccc
     2701 agcacggtgg gctgcagggt gttggccgat tcgtacgccg cctgggcatc ggggatcttc
     2761 gaggccgtca gcgaaccgcc gctgcggaac ggcacgccga gccggcgcgc gcatgcagcc
     2821 agggtgtaca ggaccagcgc aggctcgggt gtgccgaacg tcggcgcgcc cgactgcatc
     2881 gacatcgacg atgcgaacga cccgaacacc accggcgcgc cgggccgaac cagttgaacg
     2941 taggccatgc cggcgagcgc ctcggccagg gtctgggtgg cggcgccggc caccgtcgcc
     3001 ggactcatcg cccccgccag gatgaacggc gtcatgatca ccgcctggtt cgcctcggcg
     3061 taggcgcgcg cggcgccgag catcgaggag tcccacacca ggggcgagtt cgcgttgatc
     3121 aggctcaaca cgacgcagtg gtcgtccagg tagtccttgc cgaacaggat gcgcgccatc
     3181 gacaccgtgt cctcggcgcg ctgcggatgg gtcaccgacc ccatgaacgg acggtcggag
     3241 taccgcatgt gcgcgtacac catgtcgtag tgccgcttgt tcaccggcag gtcgaccggc
     3301 tcgcacaccg tgccgcccga gtggtgcagg aacggtgagg agttcgcgag cttcacgaag
     3361 ttacggaagt cctcgatcgt gccgtatcga cggccgccgt cgacgtcgcg cacgaacggc
     3421 gacccgtacg cgggcgcgaa caccgtggcg ttgccgccga tctgcacgct gtgctccggg
     3481 ttccgcgcgt gctgcgtgaa ctcccgggga gcggtggcct gcaccagttg ccggcagagg
     3541 cccgcgggga accgcacacg ttcgccgtcc acatcggctc cggcaccctt gaacaactcc
     3601 agcgcatccg gcgccccgtg gacctcgatc cccacctgct ccaggatcac gtcagcgttg
     3661 tgttcgacca ggctgagccc ttcctcatcc agcacctcga acggttgcag cgtgcgcgtg
     3721 atgaacggga tcttctcggc cacggcgtgc aaccgcgcgg cctgtcgtgc cgcgcggcca
     3781 ccgccctgtc gtcccctgcg gccttcgggt gcctgatcgc tcacgaggtg ctcctttcta
     3841 agatgtgcgg tctggggcgt cgcggcgccg tctccgggga cgatcggtgc caccggacga
     3901 ggtcctgggc gccggcagat cgatcgtcgc cgccagccgc gggaacggct cggtcgtgtg
     3961 cgggatgccc atcgccgcca cgaagtgttc ctggaaccgt ggttccaccg cggtctcgag
     4021 cttctcgacc cgccgcacca cgccctcgat ctcgccgcgg gcggcccggt tgagcaacgc
     4081 gatccgtgcg ccggtgccgg cggcgttgcc ggcgctcgtg acgcgggccg gatcgcagtc
     4141 gggcaccaga cccagcacca atgcgtacgt gggatcgatg tgggcgccga acgcgccggc
     4201 caggcggatc cgatcgaccg tctcgatgcc gaaggcgtcc atcagcaagc ggcagccggc
     4261 gtacagcgcg gccttcgcga gttggatctg gcggatgtcg ttctgggtga tcaccagacg
     4321 aggcgccccc ggcacgtcgt gcagcacgta ggagaacgtg cgcccgtcgg gttggatccg
     4381 tggcgatcgt tcgccgagcc cgccgtcgat cacaccgtcg gtggtgagga tgccggcgag
     4441 gaacagctcg gcgaccgcct cgatgatccc cgaaccgcac acgccggtga ccgttgccgc
     4501 gtcgaagccg ggatcgtccg accacgtgtc ctggccgatc acgcggaacc tcggttccaa
     4561 ggtttcgcgg tcgatgcgga cgcgttcgat cgcgcccgga gccgcccgct ggccgcacga
     4621 gatctgtgca ccctcgaacg ccgggcccgt cgggctcgac gctgcgagca tccggtcgcg
     4681 gttgcccagc acgatctccg cgttcgtccc gacgtcgacc agcaggttca cgatctcctg
     4741 gtcgtggggc gcctcggaca ggatcacgcc ggcggtgtcg gcaccgacgt ggcccgcgat
     4801 gcacggcagg acgtagacgc gagccccggc gttcgtggcc gtgagatcga gctcccgcgc
     4861 ctctcgtcgt accgcctcgt cggtggccag cgcgaacggg gcgccgccca gctccgtggg
     4921 gtcgaggccg aggaacaagt gatgcatgat cgggttgccg acgatcgtga ggtcgagcac
     4981 gtcgttgggg tcgaccccgg cccccgtgca gagctcggcg accagcccgt ccacgcactc
     5041 gcggaccacg cgggtcagtt cggtctcggc accgggattc atcatcacgt agctcacgcg
     5101 gctcatcagg tcctcgccga accggatctg cgggttcatc gcgcccgcgc tcgcgagcac
     5161 ctcgccggtg tgcaggtcgc agagatgccc ggccacggtg gtggaaccga tgtcgaacgc
     5221 cacccccaat gcgacgtcgt ggaaaccggg ccagatcgcc gtgatcgtcc acccgtcgtg
     5281 cacggccacc gtggcggtcc agtcgccggc gcgcagcgtc cgctgcagcg tggcgagcac
     5341 gtgggggtca cacgcgagcc actcgagcga ccagtcgcgc tcgagcgcct ccagcaggcg
     5401 ctgcagatcg ccgctgggat cggcgagatc gggttcgcgg acctcgacgt aatgcagacg
     5461 cacgaccggg tcgacctcga tcgggtgcgc gtcggcttcc ttgcgcacca cctggcgatg
     5521 gatctggctc tccggcggca cgtccacgac gagatcgccg cacacccggg cgtggcaccc
     5581 caggcgtcga tcgtcggcga gccccgatcg ctcgcgatag cgcgattccg gttcgctgaa
     5641 cgccgtgagg tgatcggcct tcgaggtgat gccgtgcttg gcgaactccc cctcgctcac
     5701 caccacctgg caccgtccgc agatgccacg tccgccgcac accgaatcca ggtcgacccc
     5761 cagcgcgcgc gccgcgtcca gcaggttcgt gtcctcgtcg aaccgccccc gcttgcccga
     5821 cggggtgaag acgacgagcg agccttcggc ctcactcacg gttcgggcgt cgcctccgga
     5881 tcgggaacga cgctcagcgt cggcccaccg tcggccccag caccacgacg gccggccctg
     5941 ccccctcgac ggccggtgcc gccggccggc tgctcacgat gcttcgcgat ccacgcgttg
     6001 cagtcctggt cgttgcccat catcacgtcg gcagccatca cggcctcgcg cacctcgtcg
     6061 tggagcgggc tggtgatcgc actcgtgagg ccgttcgcga tcgccatcgc caggaacgcg
     6121 ccgttcagcc ggttgcgatc gggcagtccg aacgagacgt tcgatgcgcc gcacgtgctg
     6181 ttgacgccga gctcgtcgtg gagacggcgc accaacgaga acacctgccg gcccgcgccg
     6241 cccagcgctc cgatcggcat caccagcggg tcgaccacga cgtcggcggc agcgatgccg
     6301 tgatcggcag cggcgcgcac gatcttctcg gccaccgcga accgcacgtc ggggtcttcg
     6361 ctgatgccgg tctcgtcgtt cgtgatcgcg acgacggcgg ccccgtactt ggccaccagt
     6421 ggcaacacct gctcgatgcg ctcgtcctcc ccggtcacgg agttcaccaa cggtttgccc
     6481 tcgtagacct ccaggccggc ctcgagggcg gccacgatcg aggagtcgat cgagagcggc
     6541 acgtcggtga gcgactgcac cagcttcacc gtcgcggcca ggatcgcggg ttcgtcggcg
     6601 agcgggatcc cggcgttcac gtccagcatg tggacaccgg cctcgacctg gttcacggtg
     6661 tcggcgatca ccgtggagaa gtcgcccgcc ttcatctcct cggcgagctt cttgcgtccg
     6721 gtgggattga tgcgctcgcc gatgatgacg aagggccgcc ccgacccgat cacgacctcc
     6781 ttcgtggccg aggacacgac ggtgtcggtc acgatccctc cccttcgtcg gtgccgcccg
     6841 atcgcaccag cgattccaac cgggaatcgc cgtacgcctg ttccaacgcg tgcgcttcct
     6901 tcgaggcctc ggcgtcgaga tcgtcgccgc actcgcgcgt cacccgtcgc cattcgttga
     6961 ggtactcgtc ggtgccgatc aacccgctcc gcgtggcggc catgtcgatc gcctcctgga
     7021 accgaggcga caacggaacc ttcgcgctgg tgcggccctg cttggccacc acctggacgg
     7081 ggatgtcacg ccaccagatc accgtgagat cggccatggc cccggactca cgcgttcgcc
     7141 gtggcggcgc gcttggccat cacgagcgcc tgggcggttt cggcggccac ggcggcatct
     7201 cggcaatagg catcggcccc gaccgccttg ccgaactctt cgttcagcgg agccccgccc
     7261 acgagcacga tgtagtcgtc gcggatgccc ttggccacca tctcgtcgat cacgaccttc
     7321 atgtacggca tcgtggtggt gagcaacgcg ctcatgccca ggatgtcggg cttgtgctcc
     7381 tcgagcgccg ccaagtagtc ctcgaccggg ttgttgatcc ccaggtcgaa gacgtcgaat
     7441 ccggcgccct ccaacatcat ccccaccagg ttcttgccga tgtcgtggat gtcgcccttg
     7501 acggtgccga tcacgatcgt gcccatgcgc tcggcgccgg tctcggccag cagcggccgc
     7561 aggatcgcca taccggcctt catcgcgttg gccgccaaga gcacctcggg tacgaacagg
     7621 atcccgtccc ggaagtcgat gccgacgatg cgcatgccct cgacgagcgc ctcgttgagc
     7681 acgcggtccg ccccccatcc gcgagccagc aggatgttcg tgccctcggt gatctcgtcc
     7741 gcgagcccgt cgtagaggtc gtcgtgcatc tgctgggtca ggtcctcgtc cgacagcgag
     7801 gagagatcga gttcgtcgct catgctcgtt cagcccttct cgtcaggtct tgagccgttc
     7861 gctcttggga tcgaacagtg gatcggcggt cacggtggcg gcataccgac ggccgaagta
     7921 ctcgacctcg acggcggtgc cgtgtgcagc gagcgcggtg ggcaggtagc cgtagacgat
     7981 cccctggccg atcgaatagc cgtaggccgc ggacgtcacg tagctcacca cccccccgtc
     8041 gtgccagatc ggctccttgc ccatgacgac cacgccgggg tcgtcgaacg tcatgcaggc
     8101 cagtcgctgg gccggtccgc cggcgtcgcg gatcgcttcg agcgcctcgc gcccctggaa
     8161 ctccttgtcc cagtgcaccg cgaaccccag cccggctgcg agcggatcgt gttcgatgtt
     8221 gatgtcctga ccccagagcc ggtagccctt ctccagacgg agcgaatcga aggcgccgcc
     8281 cccggccgcg atcaggccgt gcccgcggcc cgcttcccac agccggtccc agacccggtc
     8341 gcccatcgcg atctggccgt acagctccca gcccaactcg ccggcatagc tgatgcgctg
     8401 ggcccacacc gggttgacct cacccacgtt gaggtagcgc gccgtcatgt aggggaacgc
     8461 gtcgttcgag acatcggcgt cggtcacggc ttgcaggatg tctcgcgcac gcggccccca
     8521 gactcccagc gcgaacaacc cgccggtgcg ctcggtaatc cggacgacct cgccgtcgcg
     8581 aacctgcgcg cggagccacg cgaggtcgtg ctggccgctc ccgccaccgg tcaccacgcg
     8641 gaacaaccgc tcgtccttgc gggtgatcgt gaggtcgagc cggatcccac ccttcggggt
     8701 cagcgcggcg gtgtacacga tcgatcccac cgggcggtcg atccggttgg cgaacacccg
     8761 ctcgaggtag ccgagagcgt cgtcgccctc gacgtcgaac ttcgcgaacg gcgtgatgtc
     8821 gaacagcgcg gcgttcgaac gtgtggccag gtgctcggcg cccacctcgg gcgaccatcc
     8881 cctggccgcc cagcccgcgc gccgcgacca ctcgtgcgtg accccaccca cgaggtcgcg
     8941 gttggcgcgg aaccactgcg gccgctccca cccggcgccg gtgaagcact cggctccaag
     9001 cgcctgatgc cgggcgaaga acggtgtgag gcgaaggttc cgcggcttcg acggctgctg
     9061 gagtggatgg atgatgtcgt agacctcgcg gtactgctgc ttggctcgtt cgagcacgta
     9121 cggcgacgac gcctggaacg gatagaaccg gttcgcgtcg gcttcggcga ggtcgtagct
     9181 gggctcaccg tcgaccatcc attcgacggc ctgcttcgcc atgccggcgg cgtgggttac
     9241 ccacacggct tcgcacaacc agaggccacg gacggcgcca ctctcgccca ccaccgagcc
     9301 cgcgtccggc gtgaacgaga acatcccgtt gatcgaccgc gccggatcgg acggacgcat
     9361 gcggcccgcc aacgccggga acatgcgctc ggtctcggcc tcacacaggt cgaaatcgtc
     9421 gggcgtccac ggcatcagcg acggcatcgg gccatcaccg tggcgacgca gcgccgactg
     9481 gggcgtcacg atcggctcat ggcggtagtt ccccacgccg tagtgatcgt cacgctgccg
     9541 gaagtacagg gacatatcct gatggcgaac caccggctgg ctggcccagg tgtcgccggt
     9601 caacccggcg agttcgggca ccgggtccgt ccacaccagc tggtgctgga cggccaccag
     9661 cgggaccggc acccccgcca tcgcgccgac gctgggtccc cagaagccgg cgcatagcag
     9721 gacgcgatcg cactcgatac gaccctggtc ggtctccacc ccgtgcaccc ggcccgcgcg
     9781 gatatcgaag ccggtgacgg tgacgccgcc ctcgaacacg acgcccgcgg cctcggcctt
     9841 ccgcgcgagc gccgtggcga tcagcacgcc cttgccggcc ccgtcactcg gcacccagta
     9901 ggcgcccagg atcgtggagg ggtccaggag cggtgagcgt tcggcggctt ccatcggcga
     9961 gagcaactcc gtgccctcga tgccatagga ccgcgcgtag ccctggcgcc gcttcagctc
    10021 ctccatccgc tcgggcgtgg tggccacctc gatgccgccg acgccgtacc agcaccgttc
    10081 gccgtctacc tcgagggtgt cgtagagggc gaccgaatcc tgcgcgatgc ggcacatcgt
    10141 ccgcgagccg ttggtctgga agatgatccc gggggcgtgc gaggtcgatc caccggtttc
    10201 gaacagcggg ccctggtcga tcaccaggac gtcggtgacc cccatctggg acaggtggta
    10261 ggccgcgctg ctgccgacga tcccggcgcc gatgatcacc gtctgggcgc gatcccgcag
    10321 catgcccccc atgctggcag agcatgctgc ggaggcaacg tccgaggtgc gcatgccgca
    10381 acgcccgaac gggccgtcgc caggatcagt cggttccggg cgaggactcc acgatctcgt
    10441 cgggcgtgtt gtgcaccgcc gggatcgacc cgagcttgcc ggcctggaag tcctcgtagg
    10501 cctggaccag ttcctgcttc gtgttcatga cgaacggccc gtaccaagcg acgggctcgc
    10561 ggatcggccg gccgccgagc acgagcacct cgagctcggg cgagcggctc tcctgacccg
    10621 tggcggcctg cagacgcagg gcgtcgcccg caccgaacac ggcgagttgc ccggtgcgca
    10681 ccggccgcgc ttcgacaccg acggtgcctt cgccggccag cacgtaggcc agcgcgttgt
    10741 aacccgggtc ccacggcagg tcgagctgac cgcccggcga gaccgtggca tgcaccatcg
    10801 tcatcggggt gtgggtgtcg ccgggaccgg tatggctcgc gagcgaaccc gcgatcaccc
    10861 gcacgagcgc gccaccgtcg tcagaggcga gcaggcccac ttcaccggct cgcaggtcct
    10921 gatagcgggg cgccgcccac ttctcggcct tgggcaggtt cacccacagc tggatcccgt
    10981 ggaagaggcc gccgctggcc accaacgcct ccggcggctt ctcgatgtgg aggatcccgg
    11041 cgcccgccgt catccactgg gtgtcgccgt tcgtgatcgt gccgccgccg ccgttcgagt
    11101 cctgatgctc gaacgtgccg tccatgatgt aggtgacggt ctcgaacccg cgatgcgggt
    11161 gccacggcgt gcctttcggc tcacccggcg cgtactcgac ctcgcccatc tgatccatgt
    11221 ggatgaacgg gtcgagcagg gcgagatcca caccggcgaa cgcgcgccgg acggggaagc
    11281 cttcgccttc gaagccgctc ggggcggtgg tgacggtctg gacgggtcgc tggacgtgct
    11341 cgggctcggg gcgggggatg cgatcgaggg tggtgatgtc gtcgacggtg atggccggca
    11401 tggtgttggc tccttccttc gacccctcag aacggcgaac ccgccgcgcc tattcctcaa
    11461 cccagttcag cacgacggtc ttgagctcgg tgagccgctc catcgagaag cgcccgccca
    11521 cccggcccac cccgctgtgc gagcccgcgc gtccaccgaa cggcaggtgt gactcccagt
    11581 agttcgtgcc ttcgttgacg ttgacccagc ccgcgcgcac cgcctcggcg tagcgcaacc
    11641 cacgacccag gtccctggtg aagacggcgg acagcagccc gtacggccag ccgttcacga
    11701 ggtcgatcgc ctcctgttcg ctgcggatcg tggagatcgg cgccaccgga ccgaaggtct
    11761 cctcgcgcgc gatctccatg tcgttcgtca ccccgtccag gacggtggcc gagaagaaca
    11821 ggtcgctgcc gtgcttgggc acacggtcgc cgccggtgat caccttcgcg ccgtggtcga
    11881 cggcatcgcg cacgtggcgc tcggtcttgt ccgcggtcgc cgcgttgttg accggcccca
    11941 tcgtggtggc gtcgtcgaac gggtcgccca ggcggatccg gtcgtgcacc tgggcggcca
    12001 gcttggtgac gaactcctcg cggaccgcct cgtgcaccag gatgcgctcg cccgccgtgc
    12061 agctctgccc ggcgttgagg aagcatgccg ccagggtggc ctcgacggcc gcgccgacat
    12121 cggcgtcctc gaggatcacg agcgggccgt tgccacccat ctccaggagc aggtccttgc
    12181 cggcggcgcg tgaggccacc gtctccccgg tggcgatcga tccgatgaac cccaccgcct
    12241 gcgtgcccgg gttcgcggcg atctcgtctc cgacgacggc gccggggccg gtcaccatgt
    12301 tgaccacgcc gggcgggagc tcggcggcct ccagcaccgc ggccatcgcg gcggcacaca
    12361 cgctggtact gggcgccggc gcccacacca cggcgttccc gtaggcgatc gcgggcgcca
    12421 ggatctcccc cggcttcgtg tagggccagt tccacgggct gatgatcgac acgaccccgc
    12481 gcggcacccg gtagagcaac acgcgcttgt tcgcgtcgac cgacggtggc atcaagccct
    12541 ccatgcgggt ggcgtcggcg gccgccatgt cgaagtaagc gatcagttcc tcgacctcgt
    12601 cgcgcgcctc ggccacgagc ggcttgcctt gatcgagcgc gagcgtgtgc gcgaggtcgt
    12661 cccggcgctc gtcgatcacc gtcgcgatcc ggcgcatcgc ggcggcgcga tcgaaggcgc
    12721 tgagccccgc ccacgccgga gccgcggcgt tggccgcatc gatcgcccgt tgcgcgtcag
    12781 cgcgcgaacc ctcgggcacc gaaccgatca ccgcgccggt cgccgggctc gtggcatcga
    12841 aggtggcgcc gtcctcggca tcgacccacg cgccgccgat gaacatcttc acgtgctcgc
    12901 tcatgtcccc tccacagatg acggagggcg gcccgtccgg gccgccctcg tcaccgaacc
    12961 tggttcgggt gcgctgctta cgacgtgggc a
//
LOCUS       DATDBZ010000232         8432 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_283934, whole genome shotgun sequence.
ACCESSION   DATDBZ010000232 DATDBZ010000000
VERSION     DATDBZ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283934"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VIX62_05780"
     CDS             complement(<1..524)
                     /locus_tag="VIX62_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEY5859776.1"
                     /translation="MPGQIEGVKTGQSLEPSQADLIDSFARSLRARNRSPRTIKSYCD
                     TAERFVAFLQDRGMPTSAPAVTREHVEDYLADQLDRFTPSTAATRYRCLQQYFRWLDD
                     EGEIAASPMAKMSPPRVADKPVDVVDESDTRKLLDSCSGKTFDDRRDTAILMLFIDTG
                     MFSSPTCSGSPTAIS"
     gene            623..695
                     /locus_tag="VIX62_05785"
     tRNA            623..695
                     /locus_tag="VIX62_05785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:656..658,aa:Thr,seq:cgt)
     gene            complement(734..1633)
                     /locus_tag="VIX62_05790"
     CDS             complement(734..1633)
                     /locus_tag="VIX62_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEY5859777.1"
                     /translation="MSTTSVRTRPSPVLGVVLVAMVVLLAGMALVTPGAAQGEPAPAT
                     LPFPSATTVAPPPTTAVPTTVPPTTVPDPAPTPVPAPSSEPTAVRDVLLVGDSIMRST
                     GPALAHQLGARWRVHNEGVNGSGLLTPGVFPWPRHLAASLSRFDPDVVIMLFIGNYTA
                     DPAQKWVGADGRLVPDIYSRSFSREWGRQAERFVTQMIRSGSDVVLVLPPPMISARVQ
                     IVTDRLRAEYRAVASRHAEVTLADATTAVGGPHGEWVATRPTASGGWARVRVADTVHL
                     ARHGQGLVAREIRFGIARARSNA"
     gene            1847..5401
                     /locus_tag="VIX62_05795"
     CDS             1847..5401
                     /locus_tag="VIX62_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY5859778.1"
                     /translation="MKVGLLGPLEVAGPGGRVRIGGAKERMVLALLALRAGEVVSRDA
                     LVDALWGDDPPATAIKTLQGNVARVRRALEAAGMADLLLTRPPGYVLGVVPESIDVTG
                     FERHASAGRNALAVGDASSATAELGEALGLWRGDALADCRSGGWAAAQALRLDELRLS
                     TVEDRIDADLMLGQHAVLVGELESLLSRHPLRERLWAALMIALYRSGRQAEAVRAYQR
                     ARDVLVEELGLEPGAELRRLEAAVLAGDSVLDLLDATHRAPRAELSIPFPGRLGAASP
                     AVFVGRAHEREGLAMSLPVVASGQRQMVLVSGEPGIGKTSLAAAFAREAAERGAIVLY
                     GRCNEDLGIPYQPWAEALTHLVAHAPDDVLAAHVDARGNLLARLAPDLAGGTAAGPVS
                     SSDAESERYLLFGAVVDLLDRVSALAPVVLVLDDLHWADRPTIQLLRHVVSADAPLRL
                     FVIGAFRESDIDPDHPLAEALAALHRESGVERIALGGLGDDELLVLLETRAGHAMTDE
                     GVALRDALLAETDGNPFFVGEMLRHLAETRAIHEDEQGHWIVSADLRTSGLPVSIREV
                     IGRRVALLDEPTRDVLSMAAVIGSAFDADVLARVSELDEDTIIDSCDQAVTAALLTDA
                     DVAGRYAFAHALIEHALYGSRSAGRRGYTHRRVAEALEDLGGDDPAGRIGELAYHWAR
                     ATPPDVGKAIAYAQQAGDRALAQLAPDEALRWYREALDLMDRASVDDQHRRAALLLGL
                     GDAQLQTGDPAHRETLLAAGRLADDVDAVDLLVRVALRNNRGWNSVTWGFDDERIDML
                     RRALARIGDRDSADRARLLSLLCVELPVDADIDERLSLATRAVEMARRTGDKAALVDA
                     IRLGQWSLRLPQTLPLRVQWNAEACALADDLGDPNARLSAYENRLAAALESGDLATTR
                     IAWAIVKSGWERIGQPVQRWLIAVHGARQLMLEGDLAAAKQSATDALTLGTEAGYADD
                     AVTFHGAQFTCLRWMQGRSHQIIPLIEAVVHDNPDVPLLRAILASSRSFDDPHEEVRQ
                     VLETELTSGFPMLADSTWLIGQVIWADTAARSGHRPASKALYQCLLPWHDQFATGPIT
                     VQGGVAHYLGRLAHTLDLHDEADAWFSQALAFHEALEAPFFLAMTQTAWAGLLADRDQ
                     AGDAQRARALAGAALPVATERGYGYVEHDARSLLERIG"
     gene            complement(5708..6661)
                     /locus_tag="VIX62_05800"
     CDS             complement(5708..6661)
                     /locus_tag="VIX62_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5859779.1"
                     /translation="MSFTMYEEVSVSTNQPSPRTRGKVGRFRTDEAKVRFEHAYDRAL
                     ATWPMRARPVDVPTSFGRTRVNASGSESGTPIVLLHSMLATSTSWAPNIGALGADHPL
                     YAVDTICDAGRGTQERPVRNGQDLHEWFEQVLARLELDRSHLVGLSFGAWIALNHALL
                     SPARVRSVTAIEPPGAITRGRLRFLLKFVKAGMRRSDRDFDRIIELLGNGRAPSPEIV
                     DVLRCGFRDYKLALPFAKLLTDERLRSMDTPMLVLFGSESPMSDGSKAVERALSLLPD
                     VEAELVPGTAHTPPMEEPDEVNDRILRFLEVVESRRKDTVD"
     gene            6660..7475
                     /locus_tag="VIX62_05805"
     CDS             6660..7475
                     /locus_tag="VIX62_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5859780.1"
                     /translation="MYNVRVPRSRQARERPDGDSLWLLGSVPPRTSAPRRASRKDTPL
                     TRDAIVTAAIGIADLEGADAVTMRRVARDLDASPMALYWHVDNKEDLLALMLDAVEGE
                     IEIPPPTGEWRADVTKTARRYRRGLLRHGWMTNLIGVRRSLGPNELPHIERSLAAFDG
                     LDLAVRDAFNILMAIETYYLGFALREQQELAAERAVEDTTPAQRRAFFKNAGRQLADT
                     GDYPRLATLFEEGTLPSRDERFELGLARLLDGIEANLPDSRKATRRRSTRKAT"
     gene            complement(8222..>8432)
                     /locus_tag="VIX62_05810"
     CDS             complement(8222..>8432)
                     /locus_tag="VIX62_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEY5859781.1"
                     /translation="FVGGVTLPLALADLGLIDEYEFLVQPVLAGHGPTLLAGLRERIQ
                     LELVDRHEFRSGAVALRCRPTRVTA"
BASE COUNT         1324 a         2758 c         3003 g         1347 t
ORIGIN      
        1 gatatcgccg tagggctccc ggaacacgtc ggcgaggaga acatgcccgt gtcgatgaag
       61 agcatgagga tggccgtgtc ccgccggtcg tcgaaggtct tccccgagca cgagtccagc
      121 agcttgcggg tatcactttc gtccaccacg tcgacgggct tgtcggcgac ccgaggcggg
      181 gacatcttcg ccattggcga tgcggcgatc tccccctcgt cgtcgagcca acggaagtac
      241 tgctggagac accgatagcg ggtggcggct gtagacggcg tgaaccggtc gagttggtcg
      301 gcgaggtagt cctcgacgtg ctcccgggtg acggccgggg ccgaggtcgg catccctcga
      361 tcctggagaa aggccacgaa ccgctcggcg gtgtcgcagt aggacttgat cgtgcggggg
      421 gatcggttcc gagcccggag gctcctggca aacgagtcga tcaggtcagc ttgggaaggc
      481 tctaggctct gacccgtctt cacgccttcg atctgaccag gcatgtcctt cgcagcggcc
      541 gaacttgtcc gctgtgtgag cgctgcgctc gcgcaaggca gggggtaggt taggcgtttg
      601 ccctggtcac aggcgattac ccgcccgggt agctcagtcg gtagagcggc tcactcgtaa
      661 tgagcaggtc aggagttcga ttctcctccc gggctctgga atcgtggtgc aacttggagg
      721 tggcttcctc cgctcaggcg ttgctgcggg cccgagcgat cccgaagcgg atctctcggg
      781 ccaccaggcc ctggccgtgg cgggcgaggt ggacggtgtc agccacccgc acgcgggccc
      841 agccgcccga ggccgtcggc cgggtggcca cccactcgcc gtggggaccg ccgacggcgg
      901 tggtggcgtc ggccagggtc acctcggcgt gacgggaggc caccgcccgg tactcggccc
      961 ggaggcggtc ggtcacgatc tggacccggg cgctgatcat gggcgggggc agcaccagga
     1021 caacgtcgga gccggagcgg atcatctggg tcacgaaccg ctcggcctgg cggccccact
     1081 cccgggagaa ggaccgcgag tagatgtcgg gaaccagacg accgtcagca ccgacccact
     1141 tctgggcggg gtcagcggtg tagttgccga tgaagagcat gatcacgacg tcggggtcga
     1201 accggctcaa cgaggcggcg aggtgcctcg gccaggggaa cacgccgggt gtcagcaggc
     1261 cggagccgtt gaccccctcg ttgtggaccc gccagcgggc cccgagctgg tgggccaacg
     1321 ccggccccgt cgacctcatg atcgagtcac cgaccaggag gacgtcccgc accgcggtcg
     1381 gctcactgct cggcgcagga accggcgtcg gagccggatc ggggaccgtc gtcgggggca
     1441 ccgtggtcgg gaccgcggtg gtcggaggag gggcgaccgt cgtcgccgac gggaagggga
     1501 gcgtggccgg ggccggctcg ccctgggcgg cgcccggggt cacgagggcc atccctgcca
     1561 ggaggaccac catggccacg aggaccacgc cgaggaccgg ggacgggcgc gtgcgaacag
     1621 aggtggtgga catggcttgc tcctggtctc ggtgaccctc gagccaccgg gccgatcagc
     1681 gggctctcac ccgccggctc cgtcgtcctg atcccgtcga caccatccgg gcgatggtct
     1741 gtctcgatcg gggaacgagc atcgtcctga ccgtgagccg gaccggtggt agggcggtgg
     1801 tggcgtggtg gcgacccgat cgggggaggg gatctagagt gcgcgggtga aggtgggcct
     1861 actgggtccg ttggaggtcg cggggccggg gggccgggtg cggatcggcg gggccaagga
     1921 gcgaatggtg ttagccctgc tcgcgttgcg ggccggggag gtggtgtcgc gcgacgccct
     1981 ggtggatgcc ctctggggtg acgatccgcc ggccacagcg atcaagacgt tgcaggggaa
     2041 tgtggcgcgg gtgcggaggg cactcgaggc cgcggggatg gccgacctgc tgctgacgcg
     2101 gccgccgggg tacgtcctcg gtgtcgttcc cgagtccatc gacgtgacgg ggttcgagcg
     2161 gcacgccagt gcggggcgaa acgcactggc cgtgggcgac gcgtccagcg cgacggcgga
     2221 gctgggcgag gcgctcgggc tctggcgggg tgacgcgttg gctgattgtc gaagcggcgg
     2281 gtgggccgcg gcgcaggcgt tgcgcctcga cgagctgcgc ctttcgaccg tcgaggatcg
     2341 gatcgacgcg gacctgatgc tcggccagca cgccgtgctc gtgggcgagc tcgagtcatt
     2401 gctgtcgcgg cacccattgc gagagcggtt gtgggcggcg ttgatgatcg ccctgtatcg
     2461 gtccggccgc caggctgagg cggtgcgcgc gtaccagcgg gcgcgtgatg tcctcgtcga
     2521 ggagctgggc ctcgagccgg gtgccgagct tcgtcggctc gaagccgcgg ttctcgccgg
     2581 cgactccgtc ttggatctgc tcgacgccac acaccgagca ccgcgggccg agctctcgat
     2641 cccgtttccg ggcaggctcg gggcggcgtc accggcggta tttgtggggc gggcgcacga
     2701 gcgcgagggc ctggccatgt cgctgccagt cgtcgcctcg ggccaacgac agatggtctt
     2761 ggtctcgggc gagcccggga tcggcaagac gtcgctggcc gcggcgttcg ctcgggaggc
     2821 ggccgaacgc ggcgcaatcg tgttgtacgg gcgctgcaac gaggatcttg ggatcccgta
     2881 tcagccgtgg gccgaggcgc tcacccatct cgtcgctcac gcccccgacg atgtgctcgc
     2941 cgcccatgtc gatgcccgcg gcaacctgct ggcgcgactg gcgccggatc tcgccggcgg
     3001 gacggcggcc gggcccgtct cctcgagcga cgccgagtcg gagcggtatc tgctgttcgg
     3061 cgccgtggtc gacctgctgg accgtgtctc ggccttggcg ccggtcgtgc tggtcctcga
     3121 cgatctgcac tgggcggacc gtccgacgat ccagctgctg cgccatgtcg tgtcggcgga
     3181 tgcgccgcta cgactgttcg tgatcggggc gttccgggaa tccgacatcg acccggatca
     3241 ccctctcgcc gaagcgctcg ccgcgctgca ccgagagtcg ggggtcgagc ggatcgcact
     3301 gggcggcctc ggcgacgacg aactcctcgt cctgctcgaa acgagggccg ggcacgcgat
     3361 gaccgacgag ggtgtggccc tgcgcgatgc gctgttggcg gagacggacg ggaatccgtt
     3421 cttcgtcggt gagatgctcc ggcacctggc cgagacacgg gccatccatg aagacgagca
     3481 gggtcactgg atcgtgagcg cggacctgcg gacatcgggc ctgccggtca gtatccgcga
     3541 ggtgatcggc cggcgcgtcg cactcctgga cgaaccgaca cgagatgtgc tgtcgatggc
     3601 tgcggtcatc ggcagcgcgt tcgatgccga cgtgcttgcc cgggtcagcg agctcgacga
     3661 ggacaccatc atcgacagct gtgaccaggc ggtcaccgcg gcattgctca ccgacgccga
     3721 cgtggcgggc cggtacgcgt tcgcccacgc cctgatcgag cacgcgctct acggaagtcg
     3781 ctccgccggc cgtcgggggt acacccatcg gcgggttgcg gaggcgctcg aggacctcgg
     3841 tggcgatgat cccgccgggc gcatcgggga gctcgcgtac cactgggctc gcgccacgcc
     3901 cccggatgta ggcaaggcga tcgcttacgc gcaacaggcg ggcgatcggg ccctggccca
     3961 gctcgccccc gacgaggcgc tgcgctggta ccgggaggcc ctggacctta tggatcgggc
     4021 gtcggtcgac gaccagcacc gtcgggccgc gctgctcctc gggctcggcg acgcgcagct
     4081 ccagacaggt gaccccgcgc accgcgaaac cctgctcgcg gccggccgcc tggccgacga
     4141 cgtcgacgcg gtcgacctcc tcgtgcgcgt ggcgctacgg aacaaccggg ggtggaacag
     4201 cgtcacctgg gggttcgatg acgagcgcat cgacatgctc agacgcgcgc tcgcacggat
     4261 cggcgaccgg gacagcgctg accgggcgcg ccttctctcg ctgctgtgcg tcgagctccc
     4321 ggtggatgcc gatatcgacg aacgcctctc gctggcgacc cgagctgtcg agatggcgcg
     4381 gcggacaggc gacaaggccg ctttggtgga cgcgatccgg ttgggccagt ggtcgctcag
     4441 gctaccccag accctcccgc tgcgcgtcca gtggaacgcg gaggcgtgcg ccctcgccga
     4501 cgatctcggc gaccccaacg cacgcttgtc cgcctacgag aatcggttgg cggcggcact
     4561 ggaatccggc gacctcgcga ccacacggat cgcgtgggcc atcgtgaagt cggggtggga
     4621 gcggatcggc cagccggtcc agcggtggct gatcgccgtc cacggcgcgc ggcagttgat
     4681 gctcgaaggg gacctcgccg cggcgaagca gtccgcgacc gacgccctga ccctcggcac
     4741 cgaggccggc tatgcggacg acgcggtcac gttccacggt gcccagttca cgtgcctgcg
     4801 ctggatgcaa ggccgcagtc accagatcat cccgctcatc gaggcggtcg tgcacgacaa
     4861 ccccgacgtg cccctcttgc gcgccattct cgcatcgtcg agaagcttcg acgaccctca
     4921 tgaggaagtg cgtcaggtcc tggagaccga actcaccagc ggcttcccga tgctcgccga
     4981 ctccacgtgg ctcatcggcc aggtgatctg ggccgacacc gcggcgcgca gtggccaccg
     5041 acccgcctcg aaggccctct atcagtgcct gttgccatgg cacgaccagt tcgcgaccgg
     5101 acccatcacg gtccaggggg gtgtggcgca ctacctcgga cggctcgctc acaccctcga
     5161 cctccacgac gaagccgatg cgtggttcag ccaggcactc gcgttccacg aagcgctgga
     5221 ggcgccgttc ttcctcgcca tgacccagac cgcatgggcg ggcctcctcg ccgaccgcga
     5281 tcaggcgggc gatgcccaac gagcgcgcgc tctggccggc gccgcccttc ctgtcgcgac
     5341 ggagcgtggc tacggctacg tcgaacacga tgcccgctct ctgctcgaac ggatcggtta
     5401 gctctcgcgc tccagcgcga tcaggcgagc ggcttccatc ccgcgttcat cccgcgtccc
     5461 agaccgttca gtgccgtcca tggctgtcca cgaaccctgg tctggcgtgc agatccgtcc
     5521 aggaccgtcc cgtgctgtgt gggtgcagag ttctcgtaat gagcaggtca ggagttcgat
     5581 tctcctcccg ggctctggaa tctcgaggtc cggcgaggac gagatgctca cgagcgcggg
     5641 ttcgtctgtg agcctgccgt gcagctcgtg accgggcaga agttcttgtt cccagcggtc
     5701 cggagcttca gtcgacggtg tctttccgcc gggactcgac aacctcgagg aaacggagga
     5761 ttcggtcgtt gacctcgtct ggttcttcca tcggcggcgt gtgcgccgtt cctggaacga
     5821 gctcggcctc gacatcaggt agcagtgata gagcccgctc gacggctttc gagccgtcgg
     5881 acatggggct ctcggagccg aacaggacaa gcatcggggt gtccatcgat cgcaaccgct
     5941 cgtcggtcag cagcttggcg aacgggagcg cgagcttgta gtcgcggaag ccgcagcgga
     6001 ggacgtcgac gatctccggt gagggtgcac ggccgttgcc caggagctcg atgatgcgat
     6061 cgaagtctcg gtcgctcctt ctcatgccgg ctttcacgaa cttcaggaga aaccggagcc
     6121 gaccgcgggt gatcgctcct ggtggctcga tcgcggtgac gctcctcact ctggctggag
     6181 acaggagggc gtggttgagc gcgatccatg caccgaacga caagccgacg agatgagacc
     6241 gatcgagctc gaggcgggcg aggacctgtt cgaaccattc gtgcagatcc tggccgttgc
     6301 ggaccgggcg ttcctgagtg ccgcggccgg cgtcacagat ggtgtccacc gcgtacaacg
     6361 ggtggtcagc gccaagcgcc ccgatgttcg gggcccagga ggtcgatgtg gccagcatgc
     6421 tgtgcaggag cacgatgggc gtgccggatt cggagccgga tgcgttgacc cgggtacgac
     6481 cgaagctggt ggggacgtcg acaggccttg cccgcatcgg ccatgtagca agcgcacggt
     6541 catacgcgtg ctcgaaccgg accttggcct cgtcggttcg aaatcgtccg accttccctc
     6601 gtgttctcgg actcggttgg ttcgtcgaca ccgagacctc ctcgtacatc gtaaagctca
     6661 tgtacaatgt acgagtgcct cgctcccgtc aagccagaga gaggcctgac ggcgactcgt
     6721 tgtggctcct aggttcggtt ccgccccgaa ccagtgcacc gcgaagggct tcgcggaagg
     6781 acacgccact cacccgggat gcgatcgtca cggcggccat cgggatcgcc gacctagagg
     6841 gagcagacgc agtcaccatg cgacgggtcg cacgggacct cgacgcgagt ccgatggcgc
     6901 tgtactggca cgtcgacaac aaggaagatc tcctcgcgtt gatgctcgac gctgtcgaag
     6961 gcgagatcga gatcccgcca ccgacgggcg aatggcgggc agacgtcacg aagacggctc
     7021 gccgctatcg ccggggcctc ctccgccacg ggtggatgac gaacctcatc ggcgtgcgcc
     7081 gttcgctcgg gccgaacgag ctgccgcaca tcgaacgttc cctagcggcc ttcgacggcc
     7141 tggacctggc ggtgcgcgac gccttcaaca tcctcatggc catcgagacc tactacctcg
     7201 gcttcgcgct gcgcgaacag caagagctgg ccgcggaacg tgcggtcgag gacacgacgc
     7261 ccgctcaacg gcgggcattc ttcaagaacg cgggacggca gctcgctgac actggcgact
     7321 acccacgact ggcgaccctc ttcgaggaag gcacgctccc cagccgagac gaacggttcg
     7381 agctcggtct cgctcgactg ctggatggga tcgaggccaa ccttccggac tcgaggaagg
     7441 caacgcggcg gcgatccact cgaaaggcga cctgatgcgg atgcgtaccg gtcgagcatg
     7501 actgcgaccg aaccgtgtcg actgatgtat cccggtacgg cgcggcaacg actcagggcc
     7561 aggtggccat gcgcagagag gtcggacgtg ccgctagctc cagctatcgg caaggtgcgt
     7621 gctgacgctc tcgcagtcgg cgagcagtgc agaagacgcc tgtcgcggtt cgcatcctga
     7681 gtgtgacctt gctcacggat tgctaacgga ctccccggga aaggccggga cggatggttc
     7741 tgaggagatg ctgataacgg ccgaaatccc gggatttcgg gctagcccgt ccgcatcggg
     7801 agacgccggg acaggttccg accgtctcgt aatgagcagg tcaggagttc gattctcctc
     7861 ccgggctctg gagtcttgga accgggatgc cccccaaggg gcccctcgcg aggccaacga
     7921 agcggagtcc atgcggcgag cggcaacgct gtcaaccgtg catctcgtgt cggctggcgg
     7981 cggacgtggg cagcgaccgt tcgtggtgcc atgaacgcca tctgaaatag gcccccgaag
     8041 gcgaccaggg tcgccagcta gcgccgtgtc aggcaacctt ttgccctgtc ctccaacggc
     8101 acttcggggc gctgctcacg tcgatatgca cgcgcattcg gggggcacgg ccagcgcggg
     8161 gccgcgatcc gagccgtcgc tccgtcgcga ggcagcggcc aacgtgccag gacaaatcac
     8221 gtcaagccgt aactcgcgtg ggccggcatc gcagggcgac cgcccccgac cggaactcat
     8281 ggcgatccac gagctcgagc tggatgcgct cgcgcagacc ggcgagcaac gtcggcccgt
     8341 gtccggcaag gaccggctgc acaaggaact cgtactcgtc gatcagtccc agatctgcca
     8401 acgccagggg gagcgtcacg ccacccacga ac
//
LOCUS       DATDBZ010000233         2450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2733869, whole genome shotgun sequence.
ACCESSION   DATDBZ010000233 DATDBZ010000000
VERSION     DATDBZ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2733869"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VIX62_05815"
     CDS             complement(<1..496)
                     /locus_tag="VIX62_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5859782.1"
                     /translation="MERPETRYAKTADGVNIAYQVVGEGPIDILFVLGWVTHIERLWD
                     QPRIVRFLQRLASFSRVMIFDKRGVGLSDRVPEDRLPSLEVRMDDARAVMDAAGSGRA
                     IVLGHSEGGPMATLFAATYPERAIGLILFGTSVCWNGAPDYPWGNRYGTPEESTAFAE
                     ERERR"
     gene            complement(498..1337)
                     /locus_tag="VIX62_05820"
     CDS             complement(498..1337)
                     /locus_tag="VIX62_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5859783.1"
                     /translation="MNLIRNWDEPDETVDFGGVIEQLISIGGLTVARSVQPVGWRWRE
                     HFQPLVGGDWCQAHHVGVTLEGRQAILLADGTEIEYGPGDLYDIPPGHDGWTIGNEDC
                     VMLEWSGMRRWVGGATPNRVLASLLFTDIVDSTGTASRLGDAPWHDLLTLHFHGADDA
                     IERFGGRRIATTGDGILASFDAAAAAVQCGVAIGAAAREHDLTLRIGAHVGEVELAGD
                     DVRGVTVHEAARVMAAAAPGEILVSEAVRLLCQGSDLAFEDAGEHELKGVPSRWRLYK
                     VVS"
     gene            complement(1426..>2450)
                     /locus_tag="VIX62_05825"
     CDS             complement(1426..>2450)
                     /locus_tag="VIX62_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5859784.1"
                     /translation="DGGCICALFTATRPDRVRSLVIYSMDPGEGTSARPPAESEAFWE
                     ELFERIDRSWGTREYASWDLGISNPAHADDEALVSWHEAHLRLSASPATADALLRNYR
                     DTDVTGILPSIGAPTLVLHRTDNRLEPIEQSRLIARLIPNAELVELDGDDHYWVVNND
                     DIADEIETFLTGARPAPRSERVLSTVLFTDIVDSTRTAAQLGDERWRGVLDAHHEIVR
                     AELDRFAGAEVDTAGDGFLATFDGPARAVRCATSISSAVERLGIRVRAGVHTGEIEVS
                     SDDVKGIAVHIGARVAAMAGPSDVFVSQTVKDLTAGSGLVFEDAGEHELKGVPDRWRL
                     YRVMAS"
BASE COUNT          377 a          861 c          825 g          387 t
ORIGIN      
        1 accgacgctc gcgctcttcg gcgaacgccg tgctctcctc cggcgttccg tagcggttcc
       61 cccacgggta gtccggcgcg ccgttccagc agacgctggt gccgaacagg atcaggccga
      121 tcgctcgctc gggataggtg gccgcgaaca gggtggccat ggggcctcct tcggagtgac
      181 cgaggacgat cgcgcgccct gatccggccg cgtccatcac ggcgcgcgca tcgtccatgc
      241 ggacctcgag cgacgggagg cggtcctcag ggacgcggtc ggagagaccc acgccgcgct
      301 tgtcgaagat catcacgcga gagaacgacg cgaggcgctg gaggaaccgc acgatcctgg
      361 gttggtccca gagtcgctcg atgtgcgtga cccaacccag gacgaacagg atgtcgatcg
      421 gaccctcacc cacaacctgg taggcgatgt tgaccccgtc agcggtcttc gcgtagcggg
      481 tctcggggcg ctccaactca gctcaccacc ttgtagaggc gccagcggct gggcacgccc
      541 ttcaactcgt gctcaccggc atcctcgaag gcgaggtcgc tgccctggca gagcagtctg
      601 acagcctccg agacaaggat ctcgcccgga gcagccgcgg ccatcacacg ggccgcctcg
      661 tgcaccgtga caccacgcac gtcgtcgccg gccagttcca cctcaccgac gtgggcgccg
      721 atgcgaaggg tcaggtcgtg ctcgcgagcg gccgccccga tcgcgacacc gcactgcacc
      781 gccgcggcgg cggcatcgaa cgacgcgagg atgccgtcgc cggtcgtggc gatccgtcgc
      841 ccaccgaagc gttcgatcgc gtcgtcggca ccatggaagt gcagcgtgag caggtcgtgc
      901 cacggggcgt cgccgagtcg ggacgcggtg ccggtcgagt cgacgatgtc ggtgaagagc
      961 agggacgcca gcacgcggtt cggggtcgcg ccgcctaccc accgacgcat gccggaccat
     1021 tcgagcatga cgcagtcctc gttgccgatc gtccagccgt cgtgcccggg tgggatgtcg
     1081 tagaggtcgc cgggcccgta ctcgatctcg gtcccgtcgg ccaacaagat cgcttgacgc
     1141 ccctccaacg tgaccccaac gtggtgcgcc tggcaccagt cgcctccgac cagcggttgg
     1201 aagtgctccc gccaccgcca cccgaccggt tggacgctgc gtgccaccgt gaggccgccg
     1261 atcgagatca gctgctcgat cacgccaccg aagtcgaccg tctcgtcggg ctcgtcccag
     1321 ttgcggatca ggttcatcgg gcgagtctgt gaccggccgg cctcgggcgc aacctgacag
     1381 atcgctcggc cgagagtcgg tcctcgtcgg tggccgcgac ctcggtcacg atgccatcac
     1441 ccggtagagg cgccaccggt cgggcacgcc cttcagttcg tgttcgcccg catcttcgaa
     1501 cacgagccct gacccagccg tgaggtcctt cacggtctgc gagacgaaca cgtccgaggg
     1561 cccggccatc gccgcgacgc gggcgccgat atgcacggcg atgcccttga cgtcgtcgct
     1621 cgagacctcg atctcgcccg tgtgcacgcc ggcgcggacg cggatgccca gtcgctcgac
     1681 ggcgctggag atgctcgtcg cgcatcgcac cgccctcgcc ggaccatcga acgtggcgag
     1741 gaaaccgtcg ccggcggtgt ccacctccgc cccggcgaat cgatcgagct ccgcgcgaac
     1801 gatctcgtgg tgggcgtcca gtacaccccg ccagcgctcg tcgccgagct gcgcggccgt
     1861 tcgtgtggaa tcgacgatgt cggtgaacag cacggtcgag agcacccgtt cgctgcgcgg
     1921 tgccgggcgg gcgccggtca ggaacgtctc gatctcatcg gcgatgtcgt cgttgttcac
     1981 cacccagtag tggtcgtcgc cgtcgagttc cacgagctcg gcgttcggga tcagccgggc
     2041 gatgagtcga gactgctcga tgggttccag ccggttgtcg gtgcgatgga ggacgagggt
     2101 cggcgccccg atcgagggga ggatgcccgt gacgtcggtg tcccggtaat tccgcagcag
     2161 cgcatcggcc gttgccgggc tggccgagag ccgcaggtga gcctcatgcc aggagacgag
     2221 cgcctcgtcg tcggcgtggg cgggattcga gatgcccaga tcccacgacg cgtactcgcg
     2281 cgtgccccag ctacggtcga tccgctcgaa cagttcctcc cagaaggcct cgctctccgc
     2341 gggcggtcgt gccgacgttc cttctcctgg atccatcgag tagatcacca ggctccgcac
     2401 gcggtccggc ctcgttgcgg tgaagagggc gcagatgcac ccgccgtcct
//
LOCUS       DATDBZ010000234         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_896222, whole genome shotgun sequence.
ACCESSION   DATDBZ010000234 DATDBZ010000000
VERSION     DATDBZ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896222"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..787)
                     /locus_tag="VIX62_05830"
     CDS             complement(11..787)
                     /locus_tag="VIX62_05830"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEY5859785.1"
                     /translation="MDEQAPEKGSEGPDRAPSEDGRSAIDLREEWRSRNEGTTFRERL
                     DRPDRYGVLLVMIVISLISIAVFSDGEVERAFSVGILGITLVFALRTSRAPRALFRLS
                     IACVPLIVIVAVFVSRDDSQLAREAIAVITFVLLMAVLGAIIGRMTIHLTISLATVLA
                     GLCIYLLLGLVFSSVFSFLDAADSTPFFAQITTASASDLLYFSFITMTTVGYGDLTPV
                     GNLPRMLAATEALSGQIYLVTAVALLVGNLGRERRRRQPR"
     gene            complement(828..2021)
                     /locus_tag="VIX62_05835"
     CDS             complement(828..2021)
                     /locus_tag="VIX62_05835"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY5859786.1"
                     /translation="MPPSLELTNRQARRLCVMGQRLSSPRPASIGEAVHDLGMVQLDP
                     TSAVARTEHLVLFSRLGSAFRVAELERLLWDERSLFEYWVHIVPTADLPIHRATMRRY
                     PGWRGDEARSTYTKRWLEANASFRRYVLRELNRRGPLRARDLEDRTAEGWQTGGWNDE
                     RGRSVTMMLDILWFKGEVMIVGRDGQQRIWDLAERSLPMDEPKWSQAKISRALLERQL
                     RARGVATPLQFIRAFDGRTPGWERALKDLVREGVAVPVRIDGIAKGEWYAHAEVLEQA
                     WRPRTVLLSPFDDLVSDRDHTDALFDFFFRLEIYVPKAKRQWGYFVLPILHRDRLIGR
                     IDPKFDRRTNVLHVNAVHAQDGTSPADGVAAAKAIGELATWLGAGDVAYDGPVPAPWR
                     RALRA"
BASE COUNT          370 a          747 c          698 g          300 t
ORIGIN      
        1 tgacctgaag tcatctgggt tgtcgccggc ggcgttcccg ccccaggttg ccgaccagca
       61 acgccaccgc cgtcaccagg tagatctggc ctgagagcgc ctcggtggcg gccagcatgc
      121 ggggcaggtt gcccacaggc gtcaggtccc cgtaccccac cgtcgtcatc gtgatgaagc
      181 tgaagtacag caggtcggag gcgctggcag tggtgatctg ggcgaagaag ggtgtggagt
      241 cggccgcgtc caggaagctg aacaccgagg agaacaccag tcccagcaac aggtagatgc
      301 acaacccggc gagcaccgtc gcgagcgaga tcgtgaggtg gatcgtcatg cgaccgatga
      361 tcgcccccag caccgccatc aacaggacga aggtgatgac cgcgatcgcc tcgcgggcga
      421 gctgcgagtc gtcccgcgac acgaacacgg ccacgatcac gatcaggggc acgcaggcga
      481 tagagagcct gaacaaagcc cgcggcgcgc gcgaggttcg gagcgcgaac accaacgtga
      541 tgccgaggat cccgaccgag aatgcacgtt ccacctcgcc atcgctgaac acggcgatcg
      601 agatcagcga gatgacgatc atgaccagca gcacgccgta acggtcgggc cgatcgaggc
      661 gttcgcggaa ggtcgtcccc tcgttccggg agcgccactc ctcacggagg tcgatcgccg
      721 accgtccgtc ctcgctcggc gcgcgatccg gcccttcgct tcccttctcg ggagcctgct
      781 catccatggg tgagccggag tctacgccgc cgtccgtgac ggaagggtca agcgcgcaac
      841 gcgcggcgcc acggcgccgg gaccggaccg tcgtaggcga cgtcgcccgc gccgagccac
      901 gtcgcgagct cgccgatggc cttggccgcc gccacgccgt cggcgggcga cgtcccgtcc
      961 tgggcgtgca cggcgttcac gtgcaggacg ttcgtccgac ggtcgaactt cgggtcgatg
     1021 cgaccgatca accggtcacg gtgcaggatg ggcagcacga agtagcccca ctgccgcttc
     1081 gctttcggca cgtagatctc cagccggaag aagaagtcga atagcgcgtc ggtgtggtcg
     1141 cggtcgctca ccaggtcgtc gaacggcgac agcagcacgg tgcgaggtcg ccacgcctgc
     1201 tcgagcacct ccgcgtgcgc gtaccactcc cctttcgcga tgccgtcgat gcgcaccggc
     1261 acggcgacgc cctcgcgcac caggtccttc aacgctcgct cccacccggg cgtccggccg
     1321 tcgaaggccc ggatgaactg cagcggtgtc gcgacgcctc tggcccgcag ttgccgctcc
     1381 aggagcgctc gcgagatctt cgcctgtgac cacttcggct catccatcgg caggctgcgc
     1441 tcggcgagat cccagatgcg ctgctgccca tcccggccga cgatcatcac ctcgcccttg
     1501 aaccagagga tgtcgagcat catcgtcacg ctgcgcccgc gttcgtcgtt ccagccaccg
     1561 gtctgccagc cttcggccgt ccggtcctcc aagtcgcgcg ctcgaagcgg gccccgccga
     1621 ttgagttcgc gcaggacgta gcggcggaac gaagcgttcg cttccagcca gcgcttcgtg
     1681 tacgtcgaac gggcctcgtc cccgcgccag cccgggtagc gccgcatcgt ggctcggtgg
     1741 atcgggaggt cggccgtcgg gacgatgtgg acccagtatt cgaacaacga acgttcgtcc
     1801 cacaacaacc gttcgagctc agccacccgg aacgccgagc ccagccgcga gaacagcacc
     1861 agatgctcgg tgcgggccac ggccgacgtg ggatcgagct gcaccatccc caggtcgtgg
     1921 acggcctcgc cgatcgacgc gggacgcggc gacgacagcc gctgacccat cacgcacagc
     1981 cggcgggcct ggcggttcgt gagctcgagc gaagggggca tcgcggcagg ctacgctacg
     2041 gcgcgggcat gaggaacgcc gagcaccgaa cgccgagcac cgatgcgacg caggccgccc
     2101 acgatgccgc caccc
//
LOCUS       DATDBZ010000235         7458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_285299, whole genome shotgun sequence.
ACCESSION   DATDBZ010000235 DATDBZ010000000
VERSION     DATDBZ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285299"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..631
                     /locus_tag="VIX62_05840"
     CDS             437..631
                     /locus_tag="VIX62_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859787.1"
                     /translation="MNHRLDAWEMGAAIVTLTLVVAAVWLPAVAVGAGIVGVFVGLGV
                     GAWFWGTDSRDHSAGDPFGR"
     gene            679..930
                     /locus_tag="VIX62_05845"
     CDS             679..930
                     /locus_tag="VIX62_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859788.1"
                     /translation="MLEFTDRAAEALARSDAAARRFNPDARVRLVTDGAAIRAELTDH
                     AEPGDVTVRHELGFEVYVAEGIDGLVDAGEHDALFLAQT"
     gene            983..1855
                     /locus_tag="VIX62_05850"
     CDS             983..1855
                     /locus_tag="VIX62_05850"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY5859789.1"
                     /translation="MTDQGERNLSSVPGLGAGPVMVIGGAEDKVRDKVILSRFAKFAG
                     GEDGHVVVISTASSLGDQATEVYRALFTDLGIGTVTGVRPDDRDEADDPAMTAALRDA
                     TGVYLTGGNQSRLTQVVAGTKLGDALFLAHDRGAVLAGTSAGASAMASHMVAFGNAGP
                     TPKNRMVQLSAGLGILSGLVIDQHFGERGRIGRLLALVAQSPSLLGIGIDEDTCAVVF
                     SDQTLHVIGRGAVTIVDGAHVKTDAYRGKGYKPLMVSGAVLHSLPSGYWYDLRGRRLL
                     AAEHALDDEREASE"
     gene            2011..4758
                     /gene="cphA"
                     /locus_tag="VIX62_05855"
     CDS             2011..4758
                     /gene="cphA"
                     /locus_tag="VIX62_05855"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY5859790.1"
                     /translation="MQTHVFRGPNYWSYEPCIRLLVDLGSLEFWPSNTIPGFNKKLLK
                     ALPGVGEHSCSLGKKGGFKERLQDGTWMGHVAEHVALELQRETGAHISRGKTRSAGPP
                     GQYNVIYGYAEETVGLAAGALAVRLLNHLIKAEDGFDFEAEREALILLAERRQFGPST
                     QAIIDEAVTRDIPWIRLNEHSLVQLGQGTYQQRIRATMTSKTSALAVDVAGDKKMTNR
                     LLASAGLPVPRSEVVRTEDDAVVAAKRIGFPVVTKPLDGNHGRGVGLDLRTDRAVRGG
                     FKRAVSEARRGQVVVESYVTGNDYRVLVIGGHMVAIAERVPAHVTGDGKRTIAQLVEK
                     ANQDPRRGIGHEKVLTRIKIDDAARDLVKKQGFALEDVPPKGEQVLLAATGNMSTGGI
                     SIDRTWDAHEDNVEIAEEAARVVGLDVAGIDFLAPDISEAVRETGGAIVEVNAAPGFR
                     MHTHPTEGEPQYVAKSVVDGLFPPGSPSRIPILAVTGSNGKTTTTRMLAHIFRGMGRR
                     VGMTTTDGIYIDERLVKRVDASGPKSAQMVLQNPRVDTAVFEVARGGILREGLGYGRN
                     DVGVVLNVTGDHLGLREIETLEQLAAVKRVVVEAVPRNGWGVLNADDPLVLDMRRHCS
                     GNVILFTMQESHDFVDRWVRRGRKAVVLERNERGEMMVIKEGRRTMPIAWVHTLPATF
                     DGKARMMVQNAMAAAAAAHVAGAHLHDIRQGLRSFTTSIYQAPGRLNVFDLDGVKVVL
                     DYAHNAAGLETLGDFVERLAADTATGERPGEASWSANLRVAVVATAGDRRDEDMKELG
                     RVAARYFDEVIVREDTKSRGREPGETAELIREGVVEAIQGGARAGSVEVVLDEMDAAR
                     QALDRSRPGDLVVLCVDYATEVYKEIEQRRGVASPEVLRASEGDLADAVGGDPDLMRR
                     S"
     gene            4772..5104
                     /locus_tag="VIX62_05860"
     CDS             4772..5104
                     /locus_tag="VIX62_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY5859791.1"
                     /translation="MDTVAIRAELVASREDLLAELADITTVTRDPAATIGFGKRVGEG
                     TNEAIGRIERVGQADALTAKLADVERALAKLDDGTYGLCDRCGALIPAERLEARPWAV
                     LCVRCSTA"
     gene            5114..>7458
                     /locus_tag="VIX62_05865"
     CDS             5114..>7458
                     /locus_tag="VIX62_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5859792.1"
                     /translation="MGRFLFRLGGLTARHRIVVIVVWIGLAALALGLVKAYGAKTDNG
                     LTLPGTDSQAAFDILAQRFPPQQNGTNPFVFAVEEGKLTDPANRAAIAATYKAIKRSP
                     QVYSVVNPLTKDGKTVGLLSEDERIAFMPVLLKVDSGFITDRLAQAILDATKPATKAG
                     IEVAVGGSIGSVLSSPDTGESELIGNVSAMVILALVFGSLIAMGTPIVTAAFALAVGL
                     SAIGLLGHVMAVPSVAPTLATMIGLGVGIDYALFLVTKHLDQLGEGMEVEASIANAVA
                     SSGSAIVFAGSTVVIALASLAVAGIPLVTSLGFASAIAVFMAVLASITLLPAILSLLG
                     DWVRRVKVPAFLRPKPRKVGERRWDAWARGVARHPWVAVVASLVILVPLIVPLFSLQF
                     GQEDVGVTPTDTTERRAYDLLTEGFGVGYNGPLLIASTLDPVAAPSAEYTKKYDRATA
                     LQKQLKREQRRLQAQADALKQRQAELEREQAQLERKASVLQQRKGQLEQEEAALRAQE
                     ARLRAEAERLVRQAAPIAAHLAAILGRERFVQHQIDQTTDPDRLRRLRARLARLEDKE
                     ATTRARLAPYRSRAASLIAQAESLFAQAERLEQQAAALQAQADALQVQGNALQAQGEE
                     LQAQADALKRQQAQAEQQKKVALRLKRQLTAMVTKAGGDARGTDPRIVAMQDAISGTR
                     GVVGMFPPQLNKAGDALIMSAIPERAPSSPATATLVTTMREDVLPPATAGSGIDAHVG
                     GSTASNVDLAAVIAERLPLVVLTVLTLSFLLLMVAFRSLLVP"
BASE COUNT         1172 a         2479 c         2710 g         1097 t
ORIGIN      
        1 atgccggggg cgtggtcgtg cagcgacagg aagctgcgaa cggtttcgag gtcgccgggc
       61 gccgggttga ccccgctgtc ctcgtgcccc cacggcgccg tgctcatgac ggtcgttcgt
      121 tcgctcggct cacccatggt ccgcatcata ccgcaggaaa ctcccgaaat ccacttgact
      181 ggttacacct gaaaccgata taacactgat aacagttacg gattccgatc gaggaggtgg
      241 acaacgtgcc gcacatgttg ggaccaacgg cgccggagct gttgaagatc cgtgcagagg
      301 acctccgggc gcacggcgga catcgccggg agcggccggg gaggctccgc cggatgctgg
      361 ggtcgcggct gatcggcgcg ggagaacgcg tcgccggctg cacgagggca cggcccgaac
      421 cgatcaggag gatgtcatga accaccgtct ggacgcgtgg gagatgggcg ccgcgatcgt
      481 caccctcacc ctggtggtcg ccgcggtgtg gctgccggcc gtcgcggtgg gtgcgggcat
      541 cgtcggggtg ttcgtgggcc ttggcgtggg agcctggttc tggggaaccg actcccgcga
      601 ccattcggcc ggcgacccat tcggccggtg acccgttccg tctcctcgtt gaacccttct
      661 cctcgtagca tcgagcggat gctcgagttc acggatcgtg ccgccgaggc gctggcccgc
      721 agcgatgcgg cggcgcggcg cttcaacccc gatgcccggg ttcggctggt gaccgacggc
      781 gccgccatcc gcgcagagct caccgatcac gccgaacccg gtgacgtcac ggtccgtcac
      841 gagcttggct tcgaggtgta cgtggccgag gggatcgacg ggctcgtcga cgccggtgag
      901 cacgacgcgc tcttcctggc gcagacgtga acccgcgctc gctctgcggt tcggccgcgt
      961 accgggacta cacttccctg cgatgaccga ccagggcgaa cgcaacctct cctcggtgcc
     1021 cggcttggga gccggaccgg tgatggtgat cggcggtgcc gaggacaagg tccgcgacaa
     1081 ggtgatcctg tcccggttcg cgaaattcgc cggcggcgag gacgggcacg tggtggtcat
     1141 ctcgacggcg tcctcgcttg gcgatcaagc cacggaggtc tatcgggcgc tgttcaccga
     1201 cctggggatc ggcaccgtca ccggagtacg acccgacgat cgggacgagg ccgacgatcc
     1261 cgccatgacg gcggcgctcc gtgacgccac cggggtctac ctcaccggtg gcaatcagtc
     1321 gcgactcacg caggtggtgg cgggcacgaa actcggcgac gcgctgttcc tggcccacga
     1381 ccgtggggcg gtgcttgcgg gcaccagtgc cggggcgagc gccatggcga gccacatggt
     1441 ggccttcggc aacgccggac ccacgccgaa gaaccggatg gtccagctgt cggcggggct
     1501 ggggatcctg tcgggcctgg tgatcgacca gcatttcggc gagcgtggcc ggatcggacg
     1561 cctgctcgcc ctggtggcgc agtctccctc gctgctcggg atcgggatcg acgaggacac
     1621 ctgcgccgtc gtgttcagcg atcagacgct gcatgtgatc gggcgcgggg ccgtcacgat
     1681 cgtggacggg gcgcacgtca agacggacgc ctaccgcggg aaggggtata aaccgctcat
     1741 ggtgtcgggc gcggtgctgc actcgttgcc gtcgggctac tggtacgacc tgcggggtcg
     1801 ccggttgctc gcggccgagc acgcgctcga cgatgagcgg gaggcctcgg agtgacggcg
     1861 gacgccggga agcgaaccgc cgccgccgat cgagcccccg ccggggatcc tgcaccgggc
     1921 aaaggggcca cgaagacccg cgccgctcgc cagaagacca agcgcgagcc cgccgccaag
     1981 aagccggcgc tctcacccga tctcgccgtc gtgcagacgc acgtgttccg gggtccgaac
     2041 tactggtcct acgagccgtg catccgcctg ctggtcgatc tcgggtccct cgagttctgg
     2101 ccgtcgaaca cgatcccggg cttcaacaag aagctcctga aggcgttgcc cggcgtcggg
     2161 gagcactcgt gctcgctcgg caagaagggc gggttcaagg agcgcctcca ggacggcacg
     2221 tggatgggcc acgtcgccga gcacgtggcc ttggaactcc agcgtgagac cggtgcgcac
     2281 atctctcgtg gcaagaccag gagtgccggc ccgccggggc agtacaacgt gatctacggc
     2341 tacgccgagg agaccgtcgg cctggcggcg ggcgccctcg ccgtccgtct gttgaaccac
     2401 ctgatcaagg ccgaagacgg gttcgacttc gaagccgagc gggaagcgct gatcctgctc
     2461 gccgaacgcc ggcagttcgg cccctcgacg caggcgatca tcgacgaagc cgtcacccgc
     2521 gacatcccgt ggatccggtt gaacgaacac tccctggtcc agctgggcca ggggacctac
     2581 cagcagcgga tccgcgccac gatgacctcc aagacgagtg cgctcgccgt ggacgtcgcc
     2641 ggcgacaaga agatgacgaa ccgcctgctg gcctcggccg ggctgccggt cccgcgcagc
     2701 gaggtggtac gcaccgagga cgatgccgtc gtggccgcca agcggatcgg gttccccgtg
     2761 gtgaccaagc cgctggacgg caaccacggc cgcggggtgg ggttggacct gcgcaccgat
     2821 cgcgccgtac gtggcgggtt caagcgggcg gtgtccgaag cgcgccgcgg tcaggtggtg
     2881 gtggagtcct acgtcaccgg caacgactat cgcgtgctgg tgatcggcgg gcacatggtg
     2941 gcgatcgcgg agcgggtccc ggcccacgtc accggcgacg gcaagcgcac gatcgcccag
     3001 ctggtcgaga aggccaacca ggatccacgc cgtggcatcg gccacgagaa ggtgctgacc
     3061 cgcatcaaga tcgacgatgc cgcacgcgac ctggtgaaga agcaggggtt cgcgctcgag
     3121 gacgtcccgc ccaagggcga acaggtattg ctggccgcca ccggcaacat gtcgacgggg
     3181 ggcatctcga tcgaccgcac ctgggacgcg cacgaggaca acgtggagat cgccgaagaa
     3241 gcggcgcgcg tcgtaggttt ggacgtcgcc ggcatcgact tcctcgcgcc cgacatctcc
     3301 gaggcggtgc gcgagacggg tggggccatc gtcgaggtca acgccgcgcc ggggttccgg
     3361 atgcacaccc atcccaccga aggcgagccg cagtacgtgg ccaagtccgt cgtcgacggg
     3421 ttgttcccgc cgggttcgcc gagccggatc ccgatcctgg cggtgacggg gtccaacggc
     3481 aagaccacca ccacgcgcat gctggcgcac atcttccgcg ggatgggccg gcgcgtgggc
     3541 atgaccacca ccgacggcat ctacatcgac gaacggctgg tgaagcgggt ggacgccagc
     3601 ggtcccaagt ccgcgcagat ggtgctgcag aacccacgcg tggacaccgc ggtattcgag
     3661 gtggctcgcg gcggcatcct gcgtgagggc ctgggctacg gacgcaatga cgtcggggtc
     3721 gtgctgaacg tcaccggcga ccacctcggg ctgcgcgaga tcgagacgtt ggaacagctc
     3781 gcggcggtca agcgcgtggt ggtcgaagcc gtgccccgga acggctgggg cgtgctgaac
     3841 gcggacgatc cgctggtgct cgacatgcgc cggcactgca gcggcaacgt gatcctgttc
     3901 acgatgcagg agtcgcacga cttcgtcgac cggtgggtcc ggcgaggtcg caaggccgtg
     3961 gtcctggagc ggaacgaacg cggggagatg atggtgatca aggaggggcg gcgcacgatg
     4021 ccgatcgcct gggtccacac cctcccggcc accttcgacg gcaaggcccg catgatggtg
     4081 cagaacgcga tggcggccgc ggcggcggcc cacgtcgccg gcgcgcacct gcacgacatc
     4141 cgtcagggac tgcggtcgtt cacgacctcg atctatcagg cacccggtcg gttgaacgtc
     4201 ttcgatctcg acggcgtgaa ggtggttctc gactacgcgc acaacgcggc tggcctcgag
     4261 accctgggcg acttcgtcga gcggctggcg gcggacaccg cgaccggcga acgtcccggg
     4321 gaagcctcgt ggtcggccaa cctgcgcgtg gcggtcgtcg ccacggcggg cgaccggcgg
     4381 gatgaggaca tgaaggagct cgggcgggtg gcggcccggt acttcgacga ggtgatcgtc
     4441 cgggaggaca cgaagagccg cggacgcgag cctggggaga ccgcggagct gatccgcgaa
     4501 ggggtggtcg aagcgatcca agggggtgcg cgcgccggca gcgtcgaggt ggtgctcgac
     4561 gagatggacg ccgcgcgcca ggccctcgac cgctcgcgcc ccggcgacct ggtggtcctg
     4621 tgcgtcgact acgccaccga ggtgtacaag gagatcgagc agcgccgcgg ggtcgcgtcg
     4681 cccgaggtgc tgcgggcgtc cgagggcgat ctcgccgatg cggtgggcgg cgatcccgac
     4741 ctcatgcgcc ggtcgtgaac tcggaccctc gatggatacc gtcgcgatcc gtgccgagct
     4801 cgttgccagc cgtgaggacc tactagccga gctggccgac atcacgacgg tgacgcgaga
     4861 ccccgcggcc acgatcgggt tcggcaagcg cgtcggcgaa gggacgaacg aggcgatcgg
     4921 tcgcatcgaa cgggtgggcc aggccgacgc cctcaccgcg aagcttgccg atgtcgagcg
     4981 ggccctcgcg aagctcgacg acggcaccta cgggctgtgc gatcgttgcg gagcgctgat
     5041 ccctgccgag cgcctcgagg cgcggccctg ggccgtgctc tgcgttcgct gcagcacggc
     5101 ctgaggagcg agcgtgggga ggttcctgtt ccggctcggg gggctcacgg cccgccaccg
     5161 catcgtcgtg atcgtggtgt ggatcggcct ggcggcgctg gccctcggtc tggtgaaggc
     5221 ctacggcgcc aagaccgaca acggcctcac cctgccgggc accgacagcc aggcggcgtt
     5281 cgacatcctg gcccagcggt tcccgccgca gcagaacggc acgaacccgt tcgtgttcgc
     5341 ggtcgaggaa ggcaagctca ccgatcccgc gaaccgagcc gcgatcgcgg cgacgtacaa
     5401 ggcgatcaag cgctcgccgc aggtctacag cgtcgtgaac cccctcacga aggacggcaa
     5461 gacggtgggc ttgttgtccg aggacgagcg gatcgccttc atgccggtgc tgctcaaggt
     5521 cgactcaggc ttcatcacgg atcggctggc ccaggccatc ctggatgcca cgaaaccggc
     5581 gaccaaggcg ggcatcgagg tcgccgtcgg ggggtcgatc ggctcggtgc tctcgtcgcc
     5641 ggacaccggc gagagcgagc tcatcggcaa cgtctcggcg atggtgatcc tggcgctggt
     5701 cttcggcagc ctgatcgcga tggggacgcc gatcgtcacc gcggccttcg cgctcgccgt
     5761 cgggctctcg gcgatcggcc tgctgggcca cgtcatggcg gttccttcgg tggcgcccac
     5821 gctcgcgacg atgatcggcc tcggcgtggg catcgactac gcgttgttcc tggtgaccaa
     5881 gcacctcgat caactcggcg agggtatgga ggtggaggcg tcgatcgcga acgcggtcgc
     5941 ctcctcgggc agcgcgatcg tcttcgcggg cagcacggtg gtgatcgcgc tggcctcgct
     6001 ggcggtcgcc gggatcccgt tggtgacctc gctcgggttc gcctcggcga tcgccgtgtt
     6061 catggccgtg ctcgcctcga tcacgttgct gccggcgatc ctgtccttgc tgggggactg
     6121 ggtgcggcgg gtgaaggtcc ccgccttcct ccgcccgaag ccgcgcaagg tgggagagcg
     6181 gcggtgggat gcctgggcgc gcggcgtggc ccggcatccg tgggtcgccg tggtggcgtc
     6241 gttggtgatc ctggtgccgt tgatcgtccc gctgttctcg ttgcagttcg ggcaggaaga
     6301 tgtcggcgtc acgccgaccg acaccaccga gcgccgagcc tacgacctgc tgaccgaggg
     6361 cttcggcgtg gggtacaacg ggccgctcct gatcgcgagc accctcgacc cggtcgccgc
     6421 ccccagcgcc gagtacacga agaagtacga ccgcgccacc gcgctccaga agcaactgaa
     6481 gcgcgagcag cggcggctgc aggcgcaggc cgacgccctg aagcagcgcc aggccgagct
     6541 cgagcgggag caggcccagc tcgaacggaa ggcgtcggtc ctgcagcaac gcaaggggca
     6601 gctcgagcag gaagaggccg ctcttcgcgc ccaggaggcc cgcctgcgtg cggaggccga
     6661 gcggttggtc cgtcaggcgg ccccgatcgc ggcgcacctg gccgcgatct tgggacgcga
     6721 gcgcttcgtc cagcaccaga tcgaccagac caccgatccc gaccggctgc gacggctgcg
     6781 cgcccgcctc gccaggctcg aggacaagga ggcgaccacg cgcgcgcggc tcgcgcccta
     6841 ccgctcgcgg gcagcgtcgt tgatcgctca ggcggagtcg ttgttcgcgc aggcggagcg
     6901 cctcgagcag caggcggcgg ccctgcaggc gcaggcggac gcgctgcagg ttcaggggaa
     6961 cgccctccag gcccagggtg aggagctcca ggcgcaggcc gatgccctga agcggcagca
     7021 agcgcaggcc gagcagcaga agaaggtcgc gttgcgactc aagcgccaac tcaccgcgat
     7081 ggtgacgaag gcgggaggtg atgcgcgggg gaccgatccg cgcatcgtgg cgatgcagga
     7141 cgcgatctcg ggcacccggg gtgtggtcgg catgttcccg ccccagctca acaaggccgg
     7201 cgacgccctg atcatgagcg cgatcccaga gcgtgccccg tcctcacccg cgaccgcaac
     7261 gctggtcacg acgatgcgtg aggacgtgct cccgccggcg accgccggtt cgggtatcga
     7321 cgcccacgtc ggtggaagca cggcgagcaa cgtcgacctg gccgccgtga tcgccgaacg
     7381 gctcccgctc gtggtcctca ccgtgctgac gctcagcttc ctgctgctga tggtggcctt
     7441 ccgctctctg ctggtgcc
//
LOCUS       DATDBZ010000236         3880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_53010,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000236 DATDBZ010000000
VERSION     DATDBZ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53010"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VIX62_05870"
     CDS             <1..770
                     /locus_tag="VIX62_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5859793.1"
                     /translation="LRHAMSPAAAVAIARMYQATDVRDALPLIQAPTLVTYLAEDKVS
                     DAREGRYIAAHIPGATSHEVPGDDHMWRLRASEVGAVVEEFLAFREEEVAFERMLATV
                     LFTDIVGSTDRAVELGDRGWRELLERHHATVRALLGRYRGTEVVTTGDGFLATFDGPA
                     RAIRCARAIAAAMDPIGIQVRAGIHTGEIETIGDDVGGVAVHIGARVAAIAGPSEVLV
                     SSTVKDLVAGSGLTFEDAGDHELKGVPDQWHLYRVTA"
     gene            893..1732
                     /locus_tag="VIX62_05875"
     CDS             893..1732
                     /locus_tag="VIX62_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5859794.1"
                     /translation="MDHVRRWAESDEVIEFGGATEQLISIGGLTVSRTVQPPGWHWRK
                     DFQPLVGGDWCEAHHVGIGLEGRQGIRLADGTEFEIGPGELYDIPPGHDGWTIGDEAC
                     VMLEWSGMRRWVDSSGKQRMLTSLLFTDIVDSTATAARLGDVRWHDVLSMHYHQAGDA
                     IERFGGRQVASTGDGLLAAFDATAAAVRCAIALRTLGGQQNLHIRVGVHVGEVERAGD
                     DLRGLSVHAAARVMAAAGADEIFVSEPTRLLCQGAGLTFEDAGEHELKGVPDRWRLYR
                     VVT"
     gene            1732..3063
                     /locus_tag="VIX62_05880"
     CDS             1732..3063
                     /locus_tag="VIX62_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5859795.1"
                     /translation="MEVPETRYARAPDGAFIAFQVFGSGPIDVLLMPGFFTNLDENWR
                     IPEIAETHRRIGAFARVIAMDRRGMGLSDRLSPDDGAPLETHVDDVVAVLDAVGARDV
                     CIVTSESGAPILALLFAAAHPDRVRALALYTPIPSSPLVALGIDPREEMIAWGSEAFA
                     RKDLETAAPTVAGDPAAVRSWASYLRAAASPGSAFAMHEQWWETDVRGVLPTVRAPTL
                     ILTRPGASHVEFAAEFIETLGGKIPDTTLVELPGRDLPYWIGEAGAFVAEIEEFFTGT
                     RSAPDDTDRVLATVLFTDIVDSTSRAAGLGDAAWQAIRSKHDAVVRDALARHRGEEIK
                     TMGDGFLATFSGPARGVRAALEIATSVHGLGLQTRAGLHTGEITFERSDISGIAVAIG
                     ARIGAAAGPSEVLVSSTVKDLVAGSGLSFADAGEQELKGVPDRWRLYKVVG"
     gene            3148..>3880
                     /locus_tag="VIX62_05885"
     CDS             3148..>3880
                     /locus_tag="VIX62_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5859796.1"
                     /translation="MQTPETRYAWNDDVALAYQVVGDGPIDLVYLPGFCSHIDLTWES
                     PYLSAFLRGLAGRTRLIVTDRRGWGCSDRFSVNDVPPFETMTDDLLAVLDAVDSDRAA
                     IFATQECTSIACMFAATHPNRTCGLVLCDPYVTYLRTEETPWMWTTPEWEDIFRDWRA
                     TYPMPRWWTGPPGHPERAWFDRFVRASVAPGALVAEIRRFIATDIRPVLPTVAVPTLA
                     IVDPEGDEDLDPRAGAFIAERISGAE"
BASE COUNT          581 a         1291 c         1387 g          621 t
ORIGIN      
        1 cgctgaggca tgcgatgagc ccggcggctg cggtcgcgat cgcgcgcatg tatcaggcga
       61 ccgacgtccg ggacgccctc ccgctgatcc aggccccgac cctcgtcacc tatctcgcgg
      121 aggacaaggt gagtgatgcg cgggagggtc gctacatcgc cgcgcacatc cccggagcga
      181 cctcgcacga agtgccgggc gacgatcaca tgtggcgatt gcgcgcgtcg gaggtcggcg
      241 cggtggtcga ggagttcctc gcgttccgcg aggaggaagt tgctttcgag cggatgctcg
      301 cgacggtcct gttcacggac atcgtcgggt cgaccgatcg ggcggtggag ctcggagatc
      361 gtgggtggcg tgagctcttg gagcgacacc acgccaccgt ccgggcgctg ctcggtcgct
      421 accgcggcac cgaggtggtg accacgggcg acgggttcct cgccacgttc gacgggccgg
      481 cgcgcgccat ccggtgcgct cgggcgatcg ccgccgcgat ggacccgatc gggatacagg
      541 tacgggccgg gatccacacg ggcgagatcg agacgatcgg cgatgacgtc ggcggggtcg
      601 ccgtgcacat cggcgcgcgg gtagcggcga tcgcggggcc tagtgaggta ctcgtctcga
      661 gcacggtgaa ggacctcgtg gccgggtccg ggctcacctt cgaggacgcc ggcgatcatg
      721 agctcaaggg cgttcccgac caatggcacc tgtatcgggt aacggcgtga gatactgcct
      781 cttccgacag gtaccagatg acctgaaggg cgtgcccgat cgctggcacc tgtaccgggt
      841 ggtggagtag gagcggttgc gtccgagcgc gtcggggagc gacactcccc cgatggatca
      901 cgtgcggcga tgggcggagt ccgacgaggt tatcgaattc ggcggcgcga ccgagcaact
      961 catctcgatc ggcggcctca ccgtctcgcg cactgtccag cccccgggat ggcactggcg
     1021 gaaggacttc cagccgctcg tcggaggcga ctggtgcgag gcgcaccacg tcggcatcgg
     1081 cctggagggc cgccagggca tccgcctcgc ggacgggaca gagttcgaga tcggccccgg
     1141 cgagctgtac gacatcccac ccgggcacga cgggtggacg atcggtgacg aggcgtgcgt
     1201 catgctcgag tggtcgggga tgcgccggtg ggtggacagc tcgggcaagc agcggatgct
     1261 cacctcgttg ctgttcaccg acatcgtcga ctcgacggca accgcggctc gcctcggcga
     1321 cgtgcgctgg cacgacgtgc tatcgatgca ctaccaccaa gccggagacg cgatcgagcg
     1381 cttcggaggt cggcaggtgg cctcgaccgg cgacgggctc ctggctgcgt tcgacgcgac
     1441 cgcggccgca gtgcgctgcg cgatcgcgct tcgcacgttg ggtgggcagc agaacctgca
     1501 catccgcgtg ggcgtccacg tcggcgaggt cgagcgggcg ggcgacgacc tccgagggct
     1561 ctcggtgcat gcggccgccc gtgtgatggc ggcagcagga gccgacgaga tcttcgtctc
     1621 ggaaccgacc cgcctgttgt gccagggcgc gggtctcacg ttcgaggacg cgggggagca
     1681 cgagctgaag ggcgtgcccg accgctggcg cctctaccgg gtggtcacgt gatggaagtt
     1741 cccgagaccc ggtacgcccg ggcgcctgac ggagccttca tcgcgttcca ggtgttcggc
     1801 tccgggccga tcgacgtgct gctgatgccg gggttcttca cgaacctcga cgagaactgg
     1861 cggatccccg agatcgccga aacgcaccgg cgcatcgggg ccttcgcccg ggtgatcgcg
     1921 atggatcgtc gcggcatggg tctatccgac cgcctgtcgc ccgacgatgg tgcgccgctc
     1981 gagacccacg tagatgacgt ggtcgcggtg ctcgacgccg ttggcgctcg cgacgtctgc
     2041 atcgtcacca gcgagtccgg ggcgccgatc ctcgccctgc tgttcgccgc cgcgcacccg
     2101 gatcgggtgc gcgcccttgc cctctacacc ccgatcccga gcagtccact cgtcgcgctc
     2161 gggatcgatc cccgcgagga gatgattgcg tggggcagcg aggcgttcgc tcgcaaggat
     2221 ctggagacag ccgcgccgac cgtggcgggc gatccggccg ccgtccggtc gtgggcctcg
     2281 tatctccgcg cggccgcgag cccgggttct gcgttcgcga tgcacgaaca gtggtgggag
     2341 acggacgtcc gcggcgtcct gccgaccgtc cgggccccta ccctgatcct cacccgcccc
     2401 ggcgcgtcgc acgtcgagtt cgccgctgag ttcatcgaaa cgctcggtgg gaagatcccc
     2461 gacacgaccc tggtcgagct gcccgggcgc gacctgccct attggatcgg cgaggcggga
     2521 gcgttcgtag ccgagatcga ggaattcttc accgggaccc gatcggcgcc tgacgacacg
     2581 gaccgggtcc tcgcgacggt gctcttcacc gacatcgtgg actcgacgtc gcgcgcggca
     2641 ggtctcggcg acgccgcgtg gcaggcgatc cgttcgaagc acgacgccgt ggtgcgggac
     2701 gcgcttgcgc ggcaccgagg cgaggagatc aagacgatgg gcgacgggtt cctcgcgacg
     2761 ttctcaggac cggcgcgcgg ggtccgtgcc gcgctggaaa tcgcgacgtc ggtgcacggt
     2821 ctcggactgc agacccgcgc cgggctgcac acgggcgaga tcacgttcga gcggtccgac
     2881 atatcgggga tcgcggtggc gatcggcgcg cggatcggcg cggccgcggg accatccgaa
     2941 gtgctcgttt cctcgaccgt gaaggatctc gttgcaggga gcggcctctc gttcgccgat
     3001 gcgggcgagc aagaactgaa gggcgtgccc gaccggtggc gcctctacaa ggtagtgggc
     3061 tgacgtggcc atcccctcgc gacaggcacc agatgttcgg aacgccgtac ccgaccgctg
     3121 gcgcctctac cgggtggtga gcacctgatg cagactcccg agacgcggta cgcctggaac
     3181 gatgacgtcg cgctcgccta ccaggtcgtc ggagacggtc cgatcgatct cgtttacctg
     3241 cccgggttct gctcgcacat cgacctcaca tgggagagcc cctacctctc cgccttcctt
     3301 cgggggctcg ccggtcggac ccgtctgatc gtgaccgatc ggcgaggctg gggctgttcc
     3361 gatcggttct cggtcaacga cgtcccgccc ttcgagacga tgacggacga cctgttggcg
     3421 gtcttggacg cggtggactc ggatcgggcc gcgatcttcg cgacccagga atgcacgtcg
     3481 atcgcgtgca tgttcgccgc cacccacccg aacaggacgt gcggcctcgt actgtgcgat
     3541 ccgtacgtga cctatctccg taccgaagag accccgtgga tgtggaccac accggagtgg
     3601 gaagacatct tccgggactg gcgtgcgacg tatccgatgc cgagatggtg gaccggcccc
     3661 ccgggtcatc ccgagcgagc atggttcgac cggttcgtga gagcctccgt ggccccaggc
     3721 gcgctcgtcg ccgagatcag acgattcatc gcgaccgaca tccggcccgt gctgccgacc
     3781 gtggccgtgc ccaccctcgc gatcgtcgac cccgagggcg acgaggacct agacccgcga
     3841 gccggcgcgt tcatcgctga gcgcatctct ggtgccgagc
//
LOCUS       DATDBZ010000237         2294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_485896, whole genome shotgun sequence.
ACCESSION   DATDBZ010000237 DATDBZ010000000
VERSION     DATDBZ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485896"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="VIX62_05890"
     CDS             <1..1346
                     /locus_tag="VIX62_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY5859797.1"
                     /translation="DMAFPRLNAMSYWFFAGGGVLVLLSSFATDAGGVATGWYLYPPL
                     SGPRYSPGVGPDLLILGLALVAISTLMSGVNVITTTFLMRAPGMTMWRLPIFTWNAVV
                     TAILGLVAFPPAVAAFVLLLFDRRLGGHAFDPLGGGDPIVYQHLFWFLGHPEVYILIL
                     PFFGVITEIIPVFSRKRLFGYTGFVLATLAIGALSTGVWAHHMFTTGVVDNPFFSAMT
                     FTIAVPTGVKFFNWIGTMWGGKLSFRVPMLFAIGFLLNFLIGGVTGVMLASAPLDFQL
                     SDSYFLVSHFHYTMMGGSVFGIFAAIYFWWPKAFGYRLSERLGRGVFALMFVGFNLTF
                     WPQFVVGLRGMPRRIVDYAPGLGWETPNLVSSLGVVVLSAGVLLFLVDTLTSRHRRTP
                     AGDDPWGGYSLEWATSSPPPEHNFSSLPRIRSERPAFDAHHPAAAGEVARTEEPA"
     gene            1343..1726
                     /locus_tag="VIX62_05895"
     CDS             1343..1726
                     /locus_tag="VIX62_05895"
                     /inference="COORDINATES: protein motif:HMM:NF023691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEY5859798.1"
                     /translation="MKVALRYLWIYAAFGLGLGTFYYLLTEEWVGSVTLWFLGVMPLI
                     VAVWWVRHGPSAAVRASDDPDADPAASAGTSLGSFPTDTAWPVFLVLGVIVTGASLVY
                     GLLLLPVGAALLAWAIVGLARESRG"
     gene            1811..>2294
                     /locus_tag="VIX62_05900"
     CDS             1811..>2294
                     /locus_tag="VIX62_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859799.1"
                     /translation="MRTGSGQRPFVAVSLAGAVVVLLLWVGTAWATPTTRMASVSSSE
                     AHGDGSSGGYWGGSISADGRFVIFESSATNLVSNDTNGDADIFIRDRSKGTTRRISVS
                     STEAQTDGDSRRAVISPDGRFVLFVSAAANLVGNDTNAAEDVFIRDRSKGTTRRVSIG
                     N"
BASE COUNT          283 a          770 c          792 g          449 t
ORIGIN      
        1 ccgacatggc gttcccgcgc ctcaacgcga tgtcgtactg gttcttcgcc ggcggcggcg
       61 tgctcgtgct gctgagctcg ttcgcgaccg acgctggcgg cgtggcgacc ggctggtacc
      121 tctaccctcc gctgtccgga cctcgctact cccccggcgt cggcccagac ctgctgatcc
      181 tcgggctcgc cctcgtcgcg atctctacgc tgatgtcggg cgtgaacgtg atcaccacga
      241 cgttcctgat gcgggccccg ggcatgacga tgtggcgcct gccgatcttc acgtggaacg
      301 ccgtggtcac ggccatcctc gggctcgtgg cgttccctcc ggcggtggcc gcgttcgtgc
      361 tgctgctgtt cgaccgccgg ctcggcggcc atgccttcga cccgctcggc ggcggcgacc
      421 cgatcgtgta ccagcacctg ttctggttcc tgggtcaccc ggaggtctac atcctcatcc
      481 tgccgttctt cggggtgatc accgagatca tccccgtctt cagccgcaag cggctgttcg
      541 gctataccgg gttcgtgctc gccaccctcg cgatcggcgc gctctcgacg ggcgtgtggg
      601 cccaccacat gttcacgacc ggggtggtcg acaacccgtt cttctcggcg atgacgttca
      661 cgatcgccgt gcccacgggg gtgaagttct tcaactggat cggcacgatg tggggcggca
      721 agctctcgtt ccgggtgccg atgctgttcg cgatcgggtt cctgctgaac ttcctgatcg
      781 gcggggtcac cggcgtgatg ctcgcgagcg ccccgctcga cttccagctg tcggattcgt
      841 acttcctcgt ttcgcatttc cactacacga tgatgggcgg gtcggtgttc ggcatcttcg
      901 ccgcgatcta cttctggtgg cccaaggcgt tcgggtaccg cctgtcggaa cgtctcggcc
      961 gcggcgtgtt cgccctgatg ttcgtgggat tcaacctcac gttctggccc cagttcgttg
     1021 tggggcttcg cggcatgccc cggcgcatcg tcgactacgc gcctgggctc ggctgggaga
     1081 caccgaacct cgtctcgtcg ctgggcgtgg tcgtgctctc ggcgggggtg ctgctgttcc
     1141 tcgtcgacac gctcacgtcg cgacatcgcc gcacccccgc cggcgacgac ccgtggggcg
     1201 gctactcgct ggagtgggcc acgtcgtcgc ccccgcccga gcacaatttc tcgtcgctgc
     1261 cgcgcatccg ctccgagcgg cctgccttcg acgcgcatca ccccgccgct gccggtgagg
     1321 tcgcacgaac ggaggagccc gcatgaaggt agcgctgcgc tacctgtgga tctatgcggc
     1381 cttcgggctg ggactcggca cgttctatta cctgctcacc gaggaatggg tgggctcggt
     1441 cacgctgtgg ttcctcgggg tgatgccgct catcgtagcg gtgtggtggg tgcgccacgg
     1501 cccttctgcg gctgtgcgcg cgtccgacga ccccgacgcc gaccccgctg cctcggccgg
     1561 cacgtcgctc ggatcgttcc cgacggacac ggcctggccg gtgttcctcg tgctcggcgt
     1621 gatcgtcacc ggggcctccc tggtgtacgg cctgctgctg ctgccggtcg gtgcggcctt
     1681 gctcgcgtgg gcgatcgtgg gattggcacg agagagccga ggttaggggt cgaggctctt
     1741 gccagcggat ggcatggcgt gggaccgtac gcgccagcgg cggacgatcg gcggagggtg
     1801 tggtggacag gtgaggaccg gttccgggca acgaccgttc gttgcggtgt cgctggccgg
     1861 agcggtcgtc gtgctgctgc tgtgggtggg aaccgcctgg gcgacgccga cgacccggat
     1921 ggcgagcgtg agctcgtccg aggctcacgg ggacggatcg agcggcggct actggggtgg
     1981 gtcgatctcg gcagacggcc ggttcgtgat cttcgaatcc agcgcgacga acctcgtctc
     2041 gaacgacacg aacggcgatg cggacatctt catccgcgac cggtccaagg gaacgacccg
     2101 gcggatcagc gtgagctcga ccgaggcgca gaccgacggc gacagccgac gtgcggtgat
     2161 ctcgcccgat gggcggttcg tgctgttcgt ctctgcggcc gcgaacctgg tcgggaacga
     2221 cacgaacgcg gccgaggacg tcttcatccg agaccgctcg aagggcacca cgcgccgcgt
     2281 gagcatcggg aacg
//
LOCUS       DATDBZ010000238         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_692346, whole genome shotgun sequence.
ACCESSION   DATDBZ010000238 DATDBZ010000000
VERSION     DATDBZ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692346"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..587)
                     /locus_tag="VIX62_05905"
     CDS             complement(387..587)
                     /locus_tag="VIX62_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859800.1"
                     /translation="MTEAVASADDIGMRFESVVARRLLASALRVRGDIGEAAQVVEEA
                     LVIARCRGYTREVRLLSDLIEV"
     gene            complement(631..>2007)
                     /locus_tag="VIX62_05910"
     CDS             complement(631..>2007)
                     /locus_tag="VIX62_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5859801.1"
                     /translation="EGEQVDSCFVVVDGEFEVTAKGARVRTASRGDTIGDVALLTRIT
                     PHTSVTAIGAGSLMRIRRPQFLVALTGHDVDASDDGIDYEGARRRFREVVALHQRDAR
                     PGDAARPDTWLSLGAAGRMLGDPTYTDVIARGASLAKATSNGVLLAEAAAMTTWPGAF
                     FFIAENPHDEMIEVCETALTVLPRDDPMRVRVLATLASNLTFAAGPERRQSVIDEALT
                     LAGEHGDPALIGAVLNAEFICLWEPGTLERREEIADRLVEIATGTRDQELAYIGGFFA
                     AYCSAERGRLREARQQLTVLRELVPATHNQYFEFLGDRLTLSIDIALGEPGTQARIDA
                     LAVRHQATHADTDGTWALQIGGLAYQAGTLETMVSTVLAMLDVGARPTDGGRCGRGGS
                     SHRRPGRRATELLLDHRGAGTGGGCCSPWPHPSLCGPLRRAVSVPWARGHHRVGLAVF
                     RPRVAIAR"
BASE COUNT          335 a          713 c          655 g          304 t
ORIGIN      
        1 aaggcaactg gggcgcccaa gccgtcgacc tctcccccgc ccaaggagcc gtgatcgaga
       61 tgaacaccgc cgaagacggc acctacgcct tcgtcaacca cgccttcaac ttccccgggc
      121 gcggcgcgat cgggctgttc aacgcgagtt gactggccca gtctgctcct gcctacgtga
      181 gggcgagctc cttcgggatc cggcgcacct cgttgacgct gacgaaccgg ccatcgttcc
      241 tctgcttggt cgcccccgtg ccacgtcgac ctgtccgttc ggtgcccgct cgggaaggcc
      301 ttggtcgcgc tggccgcgtt ggacgcggga agactgctgc cgttcgaggc atggcacagg
      361 agtgctcggt cgtgaggcgg gacgggtcag acctcgatga ggtcgctgag caggcgcacc
      421 tcgcgcgtgt agccgcggca gcgcgcgatg acgagcgctt cctcgaccac ctgcgctgcc
      481 tccccgatgt ctccgcgcac ccggagggcc gacgccagca gccgacgggc gaccaccgac
      541 tcgaagcgca tcccgatgtc gtcggcgctc gccactgctt cggtcaacag caggcaagcc
      601 tcgtcgacgc gacccagcgc cagagcgagc tcaccgagcg atcgcgacac gaggccgaaa
      661 cacagcgagc ccgacgcggt gatgcccacg cgcccacgga acggatacag ctcgtcgaag
      721 aggaccgcac agcgacgggt gaggccaagg gctgcagcaa cctccgcctg tgcctgcgcc
      781 acggtgatcc agaagtagct ccgtggcacg tcgtcctggt cgtcgatggc tgctgccgcc
      841 cctaccgcat cgcccgccat cagttgggcg agcgccaacg tcgagcatcg ccaagacggt
      901 cgacaccatg gtttccaacg tgccggcctg gtacgcgagg ccgccgatct ggagcgccca
      961 ggtgccgtcg gtgtcggcgt gggtggcttg gtgccgtaca gcgagggcat cgattcgtgc
     1021 ctgcgtgcct ggctccccga gagcgatgtc gatcgacaac gtgagccggt cgccgaggaa
     1081 ctcgaagtac tggttgtgcg tggcgggtac cagctcgcgg agcacggtca gttgctgacg
     1141 cgcctcacgc aggcgaccac gctcggccga gcagtaggcg gcgaaaaacc caccgatgta
     1201 ggccagctcc tggtcacgag tccccgtggc gatctcgacc agccggtcag cgatctcctc
     1261 ccttcgttcg agcgtgcccg gctcccacaa gcagatgaac tcagcgttca gcacagcgcc
     1321 gatcagcgct ggatcgccgt gctcgccagc cagcgtgagg gcctcgtcga tcacggactg
     1381 gcggcgctcg ggacccgcag cgaacgtgag gttcgacgcc aacgttgcca gcaccctcac
     1441 ccgcatcggg tcgtcgcgag gcaacaccgt gagcgcggtc tcacacacct cgatcatctc
     1501 gtcatgcggg ttctcggcga tgaagaaaaa ggcgcccggc caggtcgtca tcgcagcggc
     1561 ctcggccaac agcaccccgt tgctcgtggc cttcgcgagc gacgccccac gtgcgatcac
     1621 atcggtgtac gtcgggtcgc cgagcatccg ccccgccgcg cccaagctga gccatgtgtc
     1681 ggggcgtgcg gcatcgcccg gccgtgcatc ccgctggtga agggccacca cctcacggaa
     1741 gcgacggcga gcgccctcgt agtcgatacc gtcgtccgac gcgtcgacat cgtgcccggt
     1801 cagcgccacg aggaactgcg ggcgccggat gcgcatcagc gaaccagcgc cgatcgcggt
     1861 gaccgaggtg tggggcgtga tcctggtgag cagggcgaca tcaccgatcg tgtcgcctcg
     1921 cgaggcggtg cgcacgcgtg cgccctttgc agtgacctcg aactctccgt cgacgaccac
     1981 gaagcacgaa tcgacctgct cgccctc
//
LOCUS       DATDBZ010000239         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_286284, whole genome shotgun sequence.
ACCESSION   DATDBZ010000239 DATDBZ010000000
VERSION     DATDBZ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286284"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..507
                     /locus_tag="VIX62_05915"
     CDS             130..507
                     /locus_tag="VIX62_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5859802.1"
                     /translation="MAPEDADEAQDRPVDALALKLKRTELQHQRSSIPGREIVQVLTE
                     IPVGVESGWHTHPGEEVGYILAGTVEMRIQGRSSLILHAGDPFLMPPGTPHNARDLGP
                     DTGRMLSTYIVDPGEPLASFVDV"
     gene            657..1718
                     /locus_tag="VIX62_05920"
     CDS             657..1718
                     /locus_tag="VIX62_05920"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859803.1"
                     /translation="MTTRSGRGRTAPRIPLNTFAISFGIAGLAALWSTLTAVLELPQT
                     VPLILWGGAGLAWGWLIVAHTHRGAHSTETLRSQLQHPAQGPIAAIVPAVGMLLGAEL
                     HTVWPLGGTVLALASLVAAVIFAGWMLAYWHGGNLNPEAFHGAYLLPTVAAALIGSTV
                     AARLELPGIAIGAFAVGGFFWVVLVTVLLSRLAFFPALPDALTPTLAILIAPPAVAGA
                     AWFAMNGVRADLVSTCLLGILALMLVMQLFLLGTYRRLPFTLGYWSFTFPVAAAAGYG
                     VQWLSLAPFEGWQAVAVVVAAVATVPVVAIGLRSLVLVTSVRRGARRAEQSLRRADDA
                     VRRPVPARDDATGVRPPHE"
     gene            1733..2383
                     /locus_tag="VIX62_05925"
     CDS             1733..2383
                     /locus_tag="VIX62_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY5859804.1"
                     /translation="MPDDTSPSTGIADGTRERAYAALAEADPVFRPLIEQHGTPDPFR
                     FPDGGRTADSNFAGMVLHIIAQQISTRVALVIYDRLAVATGSPPTPQSLLELTVDQLR
                     ALGMSRSKASYVRGLAEDVEVGALQIDHLDRLGDREAFDALTRVKGIGPWSAEMFLIH
                     QLRRPDILPAGDLGIRTAVRRLYALAETPPIDDVRRRGSAWAPHRTYAATLLWLSL"
BASE COUNT          378 a          869 c          822 g          417 t
ORIGIN      
        1 cgtcatcctc tccatggccc agcgtgcgaa cgccaagatc gtcgaactcg agggatcgca
       61 cgtcatcatg atctcccagc cgcaggcggt ggccgacgtc attctcgaag ctgtcgaggc
      121 gatgtcgtga tggcccccga agacgccgac gaagcacagg acagacccgt cgatgcgctg
      181 gccctgaagc tcaagcgaac cgagttgcag caccagaggt catccatccc aggcagggag
      241 atcgttcagg tgctcacgga gattccggtg ggtgtggaat cagggtggca cacgcatccc
      301 ggggaggaag tgggatacat cctggcaggt acggtcgaga tgaggatcca ggggcggtct
      361 tcgctgatcc tccacgccgg agacccgttc ctcatgccgc ccggcacgcc gcacaatgcg
      421 cgggacctgg gtccggacac gggacgaatg ctctccacct acatcgtcga tcccggcgag
      481 cccctggcca gcttcgtcga tgtctgactc ggcgcagtcg tcgatgtccg actcggcgca
      541 gaagtggacc cacccgtcgg tcttccccca cgccgacggg ctccacagcc ggtgcacatc
      601 gccgatgcgg tgcctgcggt gcgtggggtc gaagcgatgg ccgtgaggct ggatcgatga
      661 ccacgcgctc cggccggggg cgcactgcgc cgcggatccc gctcaacacg ttcgcgatca
      721 gcttcgggat cgccggcctg gcagccctgt ggtcgacgct caccgcagtg ctcgagttgc
      781 cgcagaccgt cccgttgatc ctctggggcg gcgcgggcct cgcgtggggg tggctcatcg
      841 tcgcgcacac gcacaggggt gcgcacagca cggagacgct gcgatcgcag ctccagcacc
      901 cggctcaagg ccccatcgca gcgatcgtgc cggcggtcgg catgctcctc ggcgcggagc
      961 ttcacacggt ctggccgctg ggtggcacgg tgctcgcact ggcatcgctc gttgcggcgg
     1021 tgatcttcgc cgggtggatg ctcgcctact ggcacggcgg gaatctgaat cccgaagcgt
     1081 tccacggggc gtacctgctg ccgacggtcg ccgcagcact catcgggtcc acggtcgcgg
     1141 caagactcga gctccccggc atcgcgatcg gcgcgttcgc ggtcggcggc ttcttctggg
     1201 tcgtcctggt caccgtgctg ctgtcgaggc tcgcgttctt ccccgcgctg ccggacgccc
     1261 tcacgcccac cctggcgatc ctcatcgcgc cgccggcggt cgccggagcg gcatggttcg
     1321 ccatgaacgg agttcgggcg gacctcgtct cgacctgcct gctcggcatc ctcgccctca
     1381 tgctcgtcat gcagctgttc ctgctcggta cgtaccgtcg gttgccgttc acgctcggct
     1441 actggtcgtt cacgttcccc gtcgcggcgg cggccgggta cggcgtccaa tggctcagcc
     1501 tggctccatt cgaaggatgg caggcggtgg ccgtcgtcgt tgcggccgtc gccacggtgc
     1561 ccgtcgtcgc catcggcctc cgctcgctcg tcctcgtcac gagcgttcgg cgcggagccc
     1621 ggcgtgcgga gcagtccctt cgacgcgcgg acgacgcggt acgtcgtccc gttcccgcac
     1681 gagatgatgc aaccggagtt cgaccgccgc acgaatgaca gatcggccca gcatgccaga
     1741 cgacacctca ccatccacag ggatcgcaga cggaacgcgt gagcgcgcgt atgccgcgct
     1801 cgcggaagcc gacccggtgt tccgacccct catcgagcag cacggaacac ccgatccgtt
     1861 ccggttccca gacggcggtc gcaccgcgga cagcaatttc gcaggcatgg tgctgcacat
     1921 catcgcccag cagatctcga cgagggtggc gctcgtcatc tacgaccggc tcgccgtggc
     1981 gacgggatcg ccaccgaccc cgcagagcct gctcgagctg acggtcgacc agctgagagc
     2041 gctggggatg tcgcgctcga aggcgagcta cgtgcgcggc ctcgcagaag acgtcgaagt
     2101 cggcgcgctc cagatcgacc acctggatcg gctgggcgac cgagaggcct tcgatgccct
     2161 gacgcgagtg aagggaatcg ggccgtggag cgctgagatg ttcctcatcc accagctcag
     2221 gcgccccgac atcctccccg ccggcgactt gggcatccga accgccgtcc gacgcctgta
     2281 tgcactggcc gagacgcctc ccatcgacga tgtgcgacga cgcgggtcgg cgtgggcacc
     2341 tcatcgaacc tacgccgcga cgctcctgtg gctctcgctc tgaatcggcg gggtctggtc
     2401 tgatctcgcc ggtgacgctg cccgattcgc ctgtcgacgt cgtgcatcgc gtgcgacgat
     2461 cgggctatga agcaacggga acgccg
//
LOCUS       DATDBZ010000240         2302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_486419, whole genome shotgun sequence.
ACCESSION   DATDBZ010000240 DATDBZ010000000
VERSION     DATDBZ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486419"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VIX62_05930"
     CDS             complement(<1..341)
                     /locus_tag="VIX62_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5859805.1"
                     /translation="MTARYAFPIDRFQADAFDSLDRGDHVVVAAPTGSGKTVVAEYGI
                     AMARAAGHRAFYTAPIKALSNQKYRDLVAIHGDDAVGLLTGDNAINGDAPIVVMTTEV
                     LRNMIYGRSRAL"
     gene            complement(398..1177)
                     /gene="tatC"
                     /locus_tag="VIX62_05935"
     CDS             complement(398..1177)
                     /gene="tatC"
                     /locus_tag="VIX62_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY5859806.1"
                     /translation="MTTDNRQADQPPGDTMTLTEHLGELRARIIRAALAITIGMVLVV
                     AFYDQVLGFLLEPYQELCETKPPDFCTSELFNFTPTEGLSTRLRVGMYGGIILALPVI
                     LWQVWRFIVPALNPKEKKYAIPFVVSSIVLFLSGGALAYLTIGQALDFLISWSGDDVN
                     QVYSVNSYVALIGLMIFAFGLGFLLPVLVVFLQLVGVVTPRRLMQSWRLAMVGIAVIA
                     AVITPSGDPITMAMLGVPMMVLYFVAVLIGWLVVRRRPQDE"
     gene            complement(1174..1800)
                     /gene="tatB"
                     /locus_tag="VIX62_05940"
     CDS             complement(1174..1800)
                     /gene="tatB"
                     /locus_tag="VIX62_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY5859807.1"
                     /translation="MFNLQGSEIIVILVLALVVLGPEKLPGAIKQFTKTYAELKKMGN
                     GFQSELKDALEEPMREMRETANIVRDATDPNKIMAEAEAEQRLEHTRVREDASPVADA
                     PPATEWADGAFPSPSPGVLPASGAPPPPPPTAAAAVAGAAASPTPPPPAPPPAPPSLA
                     EVTPPPRLPSSPAASPAPPPPPPPPPPPPPARPPSPAPFPSRRSESSA"
     gene            1836..>2302
                     /locus_tag="VIX62_05945"
     CDS             1836..>2302
                     /locus_tag="VIX62_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY5859808.1"
                     /translation="MQQRLPSRLDPPIAFAHRGARAHARENTIEAFELALRLGATGLE
                     SDVWLTADGVAVLDHDGVAKIRRRKRPIRELLRSQLPGHIPSLAEMLAACGTGYALSL
                     DLKDPAAGPEILRTVREHDPAMVEHTWLCDIDLPRLRALRAAEPDARLLLSTRL"
BASE COUNT          361 a          748 c          830 g          363 t
ORIGIN      
        1 agggcccgtg accgcccgta gatcatgttg cggaggacct cggtcgtcat gacgacgatc
       61 ggggcgtccc cgttgatggc gttgtccccg gtgagcagcc cgacggcgtc gtcaccgtgg
      121 atggcgacga gatcgcggta cttctggttc gacagcgcct tgatcggggc ggtgtagaac
      181 gctcggtgcc cggcggcgcg agccatggcg atgccgtatt cggcgaccac ggtcttgccg
      241 cttccggtcg gcgccgccac gacgacgtgg tcaccgcgat cgagcgagtc gaacgcgtcg
      301 gcctggaacc ggtcgatcgg gaacgcgtag cgcgcggtca gctcagcgcg gccgggcgtg
      361 cgcgacgccg ttggttcgcc ggagcgtggc gtcgaggtca ctcgtcctgg ggtcgccttc
      421 gcacgaccag ccaaccgatg agcacggcca cgaagtagag caccatcatc gggacgccga
      481 gcatcgccat ggtgatgggg tcgccggacg gagtgatcac ggcggcgatc accgcgatgc
      541 cgaccatcgc gaggcgccag ctctgcatca gccgacgcgg tgtgaccacg ccgacgagct
      601 ggaggaagac gacgagcacc gggagcagga agccgagccc gaacgcgaag atcatcaagc
      661 cgatcaacgc cacgtagctg ttcaccgaat acacctggtt gacgtcgtcg ccggaccagg
      721 agatgaggaa gtcgagcgcc tgcccgatcg tgaggtaggc gagtgcgcca ccggagagga
      781 acagcacgat cgacgacacc acgaacggga tggcgtactt cttctccttg gggttcaagg
      841 ccggcacgat gaagcgccag acctgccaca ggatcaccgg gagggccaga atgatgccgc
      901 cgtacatgcc gacgcggagc cgggtgctga ggccctccgt cggtgtgaag ttgaacagtt
      961 cggacgtgca gaagtcgggg ggcttcgtct cgcagagttc ctggtagggc tcgagcagga
     1021 acccgagcac ctgatcgtag aacgcgacca cgagcaccat gccgatcgtg atggcgagcg
     1081 cggcgcggat gatccgggcg cgcaactcac cgaggtgctc ggtgagagtc atcgtgtcgc
     1141 cgggcggttg atctgcctgg cggttgtccg tcgtcacgcc gacgactccg atcgccgtga
     1201 cggaaacggc gcgggcgacg gcggacgcgc gggcgggggt gggggtggtg gtggcggcgg
     1261 cggaggcggc gcgggtgacg cggcgggcga ggacggcagt cgaggcggtg gcgtcacctc
     1321 cgccagcgat ggcggtgccg gtggtggcgc cggcggaggc ggagtcggcg atgctgctgc
     1381 tcctgccact gcggctgctg ctgttggcgg tggcggcggt ggagctccgg acgccggcag
     1441 cacgccgggc gacggggacg ggaacgcccc gtcagcccac tcggtggccg gtggggcgtc
     1501 ggcgaccggc gacgcatcct ccctcacccg ggtgtgctcg aggcgttgtt cggcttcggc
     1561 ttcggccatg atcttgttcg gatcggtcgc atcgcgcacg atgttggcgg tctcgcgcat
     1621 ctcccgcatc ggctcttcga gcgcgtcctt cagttccgac tgaaagccgt tccccatctt
     1681 cttcaactcg gcgtaggtct tcgtgaactg cttgatcgcg cccggcagct tctcgggccc
     1741 gaggacgacg agcgccagga ccaggatcac gatgatttcg ctgccctgga ggttgaacac
     1801 gctggcagtc tacgggcctg gcatcatctc tcggagtgca gcagcgcctc ccctcacgtc
     1861 tcgatccacc gatcgcattc gcccaccgtg gcgcacgcgc gcacgcgcgc gagaacacga
     1921 tcgaggcgtt cgagctggcg ctccgcctcg gcgcgaccgg tctcgagagc gacgtctggc
     1981 tgaccgccga cggtgtcgcg gtgctcgacc acgacggcgt cgcgaagatc cgccgccgga
     2041 aacggccgat ccgcgaactc ctgcgcagtc agctgccagg tcacattccg tcgctggccg
     2101 agatgctcgc cgcgtgcggc acggggtacg cgctgtctct cgacctcaag gatcccgcgg
     2161 cggggcccga gatcctgcgc acggtccgtg aacacgatcc ggcgatggtc gagcacacct
     2221 ggttgtgtga catcgatctc cctcggctcc gagcgctgcg cgccgccgag ccggacgccc
     2281 gactgctgct ctcgacccgc ct
//
LOCUS       DATDBZ010000241         4909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_286930, whole genome shotgun sequence.
ACCESSION   DATDBZ010000241 DATDBZ010000000
VERSION     DATDBZ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286930"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VIX62_05950"
     CDS             <1..685
                     /locus_tag="VIX62_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY5859809.1"
                     /translation="LDSGIDASHPWVGSVEGGVALEYDPDAAGGVRFEEGPHEDLYGH
                     GTACAGIIRRLAPDCEIYSVRVLGSKLTGKGFVFAAGLDWALREGMRVVNLSLSTGKR
                     DYYSVFHRLADEAVFKRVMLVCAINNMPGPSFPAQYSSVFSVASHDEVGDPFAFDFNP
                     KPPAEWGAPGIDLEVPWLDRKTIVATGNSFAAPHISGILTLILAKHPELTPFQAKTVL
                     MALARNVHA"
     gene            723..1634
                     /locus_tag="VIX62_05955"
     CDS             723..1634
                     /locus_tag="VIX62_05955"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY5859810.1"
                     /translation="MTYSIVARDPATGRFGVAVQSHWFAVGRIVPWAEAGVGAVATQS
                     FAEASYGPLALDLMRRGKSAREAMDALLLVDDEREARQVAIVDAAGDVATHTGEDCLS
                     LAGHRGGDGYSVQANIMERDSVWPAMAQAYEGADGDLADRLFAALRAAQAEGGDLRGM
                     QSAAILIVEAAPTGKEWVDRVLDLRVEDHDAPLDELERLIRRWRAYGHAEMAEDAELS
                     QDAARALTQRLTALEIEPDNPEMAFWAAVALADAGRTEEARAALQPVRPVGDRWAELL
                     RRCIAEGALELDPGTAEMLTAELEGRS"
     gene            complement(1647..2339)
                     /locus_tag="VIX62_05960"
     CDS             complement(1647..2339)
                     /locus_tag="VIX62_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5859811.1"
                     /translation="MTTEDNGRLLASSSLFSGLEAKVLASLGERAVRQQFEKGRTIFY
                     QGDPGDTLYVVAEGLVKVWVSSHDGSEMVMATLRPPDAFGELSAVDGHVRSASATTLE
                     PTVLLSLDRTTLLDAVHHNAAVADALLRALGSLARRITEQASDLVFLDLAGRVAKCLV
                     HLAVREGHVDGETTVLELPLSQTEIAEMVGGSRQSVNQTMKNFEMRGYLQLRGREVVI
                     LDLASLQHRAER"
     gene            complement(2339..2767)
                     /locus_tag="VIX62_05965"
     CDS             complement(2339..2767)
                     /locus_tag="VIX62_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5859812.1"
                     /translation="MPDEEKMQALKAVPLFQGFSDKDLARVAEITKEVHHEDGEFVVE
                     EDRSAVGFHLILSGEADASTGGEVVNTMGPGDFFGEMSLIDGQPRSATVTASGPLTTL
                     AIPAWNFNRLLDEHPEMMRALLVELSARIRNLRDRHPSRG"
     gene            complement(3063..3920)
                     /locus_tag="VIX62_05970"
     CDS             complement(3063..3920)
                     /locus_tag="VIX62_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY5859813.1"
                     /translation="MEPGDVRTLGVVGCGLMGSGIVEIAARVGIDVVYVEPTEDLVAA
                     GRTRIEGSTAKAVERDKLTPDDRDALLARIRGGSDLATFADVDLVIEAATEDPSIKLD
                     TFRTLDGIVRPEVVLGSNTSSIPIATLGAATGRPDRVIGIHFFNPPPVMKLVELTPSL
                     STSPETVTFARGFGERLGKTVVIAKDHAGFIVNRLVIPYVCAAIRLYDDGFATREDID
                     VSMTLGMNHPMGPLKLADFIGLDTVLQIAEVLHAEFRDPMYAPPPRLQRMVDAGHLGR
                     KSGRGFYEY"
     gene            3984..4553
                     /locus_tag="VIX62_05975"
     CDS             3984..4553
                     /locus_tag="VIX62_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859814.1"
                     /translation="MHDGNARRTGVILGVAAAGLLLGHWLTYLLDVPSHARDQVLQAT
                     GHGYLATVGALAAVTAAVSLAIVFLGRLTRREPGARFIAVAGRLAAVQMSAFVAMEVL
                     ERLGAGAPMHDLATILPVGVAAQAVVALAGAWLLRLVLRAAEVAGSLATSLPALLPRA
                     ERIIAPAVFAPPPGLWLQRRSSRAPPLPS"
     gene            4621..>4909
                     /locus_tag="VIX62_05980"
     CDS             4621..>4909
                     /locus_tag="VIX62_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859815.1"
                     /translation="MRRSTFRLAALCAAVAMLVLIPAGAALAHGHTSQGDLEMEIGFA
                     TEPAYTGQPNAVQLLLVHDGHPVTDLKTGDITVEVSFGDQTSEPMDMEPWFF"
BASE COUNT          733 a         1724 c         1693 g          759 t
ORIGIN      
        1 gctcgattcg ggcatcgacg catcgcatcc gtgggtcggc agcgtcgaag gcggcgtcgc
       61 cctggagtac gacccggacg cggcgggcgg cgtccggttc gaggagggac cgcacgagga
      121 tctctacggc cacggcaccg cctgtgccgg gatcatccgc aggctcgcgc cggactgcga
      181 gatctacagc gtgcgcgtgc tggggagcaa actgaccggc aaggggttcg tgttcgcggc
      241 gggcttggac tgggcgctgc gggaaggcat gcgcgtggtg aacctgtcgt tgtccacggg
      301 caagcgcgac tactactcgg tgttccaccg gctcgccgac gaggcggtgt tcaagcgcgt
      361 gatgttggtg tgcgcgatca acaacatgcc gggcccgagc ttccccgcgc aatattcgtc
      421 ggtgttctcg gtcgcctcgc acgacgaggt gggcgatccg ttcgccttcg acttcaaccc
      481 gaagccgccg gcggagtggg gggcgcccgg gatcgacctc gaggtgccat ggctggaccg
      541 caagacgatc gtggcgaccg ggaactcctt cgccgcgccg cacatcagcg gcatcctcac
      601 gctgatcctg gccaagcacc cggagctgac gccgttccag gccaagacgg tgctgatggc
      661 gctggcgagg aacgtgcacg cgtgaacgcc ccgacagatg cgcgggtggg agaaggcatc
      721 ccatgacgta ctcgatcgtg gcccgcgacc cggcgaccgg tcggttcggc gtggccgtgc
      781 aatcccactg gttcgcggtg ggccggatcg tgccgtgggc cgaggccggc gtgggcgccg
      841 tggcgaccca gtccttcgcc gaggcctcgt acgggccgct cgcgctcgac ctgatgcgcc
      901 gcggcaagtc cgcccgcgag gcgatggacg ccctgctgct ggtcgacgac gaacgcgagg
      961 cgcggcaggt ggccatcgtc gatgccgccg gcgacgtggc cacccacacg ggcgaggact
     1021 gcctcagcct cgccggtcat cgcgggggcg acggctacag cgtgcaggcc aacatcatgg
     1081 agcgcgactc cgtgtggccg gccatggcgc aggcgtacga gggcgccgac ggggacctcg
     1141 ccgatcgcct gttcgccgcc ctccgggccg cgcaggccga gggcggcgat ctccgaggca
     1201 tgcagtccgc cgcgatcctg atcgtggagg cggcgcccac cggcaaggaa tgggtggatc
     1261 gcgtcctcga tctccgggtc gaagaccacg acgctccgct ggacgagctc gagcggctga
     1321 tccgacggtg gcgcgcctac ggtcacgccg aaatggcgga ggacgcggag ctctcccagg
     1381 acgccgcgcg ggccctcacc cagcggctga ccgccctcga gatcgaaccc gacaatccgg
     1441 agatggcctt ctgggcggcc gtggcgctgg ccgacgccgg acgcaccgag gaagcgcgcg
     1501 ccgccctgca gccggttcga ccggtgggcg atcgctgggc ggaactgctt cggcggtgca
     1561 tcgccgaagg cgcgctggaa ctcgaccccg ggaccgccga gatgctgacg gccgagctcg
     1621 agggtcgctc ctgaagcgtc agccgctcat cgttcggcgc gatgttggag cgaggccagg
     1681 tccaagatca cgacctcgcg accgcggagc tgcaggtacc cgcgcatctc gaagttcttc
     1741 atcgtctggt tcacgctctg ccgcgatccg ccgaccatct cggcgatctc ggtctgcgac
     1801 agcggcagct cgagcacggt ggtctccccg tccacgtgcc cttcccgaac ggccaggtgc
     1861 accaggcact tggccacccg tccggcgagg tcgaggaaca ccaggtcact ggcctgctcg
     1921 gtgatccggc gcgcgaggct gcccagggcg cgcaggagcg cgtcggccac ggcggcgttg
     1981 tggtggacgg cgtcgagcaa cgtggtgcga tcgagcgaga gcagcaccgt cggctccagg
     2041 gtggtggccg acgccgagcg cacatggccg tccacggcgg agagctcccc gaatgcatcg
     2101 ggcgggcgca aggtcgccat caccatctcg ctgccgtcat gggaggagac ccacaccttg
     2161 accagaccct cggctaccac gtacagggtg tcccccggat cgccctggta gaagatcgtg
     2221 cggcctttct cgaattgctg gcggaccgct cgttctccca gcgaagccag gactttggcc
     2281 tcgaggccgg agaacaggga cgaactcgcg agcagccgtc cgttgtcctc ggtggtcatc
     2341 agccgcggct gggatggcgg tcgcgcaggt ttcggatccg cgcgctgagc tcgaccagca
     2401 acgcccgcat catctcggga tgctcgtcca gcagacgatt gaagttccag gccgggatcg
     2461 cgagcgtggt cagcggcccg ctggccgtga cggtcgccga acgcggctgg ccgtcgatca
     2521 gcgacatctc gccgaagaag tcccccgggc ccatcgtgtt gaccacctca ccaccggtgg
     2581 acgcgtcggc ctcgccgctg aggatcaggt ggaacccgac ggccgaccga tcctcctcca
     2641 cgacgaactc accgtcttcg tggtgaacct ctttggtgat ctccgcgacc cgcgccaggt
     2701 ccttgtcgga gaacccctgg aacaggggaa ccgccttgag ggcctgcatc ttctcctcgt
     2761 ccggcatgga gcctccctgc gtggatcgct cggtcgtcgc ggtcgtgtcg gcagcatagc
     2821 ccgggaccct gggcatcggg aaccggctag accggaccaa cgggtgaacc aggccggcgc
     2881 gacggggaga cgaagacccc ggggcctcgc ccgcgagggc cccggggaat ccgatggccg
     2941 agggttcagg gggtccagat cccgcgtgcc tggcggcatc gtcgtgcccg ggcccgcccg
     3001 aggctgtcag ccagcggaca ccggaggtgt cagcttgccg acaccggtac cagcgcggtt
     3061 gctcagtact cgtagaatcc gcggccgctc ttgcggccga ggtgcccggc gtccaccatc
     3121 cgttgcaacc gaggcggggg cgcatacatc gggtcgcgga actccgcatg gagcacctcg
     3181 gcgatctgca acacggtgtc gagcccgatg aaatcggcca gcttcaacgg acccatcgga
     3241 tggttcatcc ccagggtcat cgagacgtcg atgtcctcgc gggtggcgaa cccgtcgtcg
     3301 tagagacgga tcgccgcaca gacatatggg atcaccagcc ggttcacgat gaagccggcg
     3361 tggtcctttg cgatcaccac ggtcttgccc agccgctcac cgaacccgcg cgcgaacgtg
     3421 acggtctccg ggctggtcga cagcgacggc gtcagctcca ccagcttcat caccggtgga
     3481 ggattgaaga agtggatgcc gatcacgcgg tcgggacggc cggtcgccgc gcccagggtc
     3541 gcgatcggga tcgacgacgt gttggagccg agcacgacct ccggtcgaac gatcccgtcg
     3601 agcgtccgga acgtgtcgag cttgatcgag gggtcctcgg tcgccgcctc gatcacgaga
     3661 tcgacatcgg cgaaggtcgc caggtcgctc cccccgcgga tccgggccag cagggcgtcg
     3721 cgatcatccg gggtgagctt gtcgcgttcg acggccttgg cggtggagcc ctcgatgcgg
     3781 gtgcgcccgg ccgcgaccag gtcctcggtc ggttcgacgt agacgacgtc gatgcccaca
     3841 cgcgcggcga tctccacgat gcccgagccc atcagcccgc agcccacgac ccccaaggtc
     3901 cgaacgtccc ccggttccac gatcctcttc cctgtctccg atgacttgcc ggtgatgtct
     3961 gggcccagcc tacaatcgtg gccatgcacg acgggaacgc gcggcgcacc ggggtgatcc
     4021 tcggcgtggc cgctgccggg ctgctgctgg gtcactggct cacctacctg ctcgacgtcc
     4081 cgtcgcacgc ccgggaccag gtgctgcagg ccaccggcca cgggtacctc gccacggtgg
     4141 gggccctggc cgccgtcacc gccgcggtgt cgctggcgat cgtcttcctc ggacgcctca
     4201 cccggaggga acccggggcg cggttcatcg ccgtcgcggg acgcctcgcg gccgtccaga
     4261 tgtcggcctt cgtggccatg gaggtcctcg aacgcctggg cgccggtgcc ccgatgcacg
     4321 atctcgccac gatcctgccc gtcggtgtgg cggctcaggc cgtcgtggcc ctcgccggag
     4381 catggctgct gcgcctggtg ttgcgcgcgg ccgaggtcgc cggttcgctc gcgacgtcgc
     4441 tacccgccct ccttcctcgt gccgagcgga tcatcgctcc cgccgtgttc gccccgcccc
     4501 cagggctgtg gctccagcgc cgctcctccc gggctccgcc cctgccgtcg tgacctaaag
     4561 ctgtcctcag ccggtccatc ggatcggttg agggctccat cacgacgaag gagacccttc
     4621 atgcgacgtt ccacgttccg gctggcggcg ctctgcgccg ccgtcgcgat gctcgtcctc
     4681 atcccggccg gcgccgcgct cgcgcacggc cacacgtccc aaggcgatct ggagatggag
     4741 atcgggttcg cgaccgaacc cgcgtacacc ggccaaccca acgccgtgca gctcctactg
     4801 gtgcacgacg gccatcccgt caccgacctc aagaccggcg atatcaccgt ggaggtgagc
     4861 ttcggcgacc agacctccga gcccatggac atggaaccgt ggttcttct
//
LOCUS       DATDBZ010000242         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_693861, whole genome shotgun sequence.
ACCESSION   DATDBZ010000242 DATDBZ010000000
VERSION     DATDBZ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693861"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1277
                     /locus_tag="VIX62_05985"
     CDS             63..1277
                     /locus_tag="VIX62_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859816.1"
                     /translation="MHPVRYFKPWRMWRRHDLAKTYDVIIIGGGAHGLAIAYELAKRG
                     ITNGAVLEKDYIGAGGSGRNTTIIRANYRTPEGVAFYKEALRLYEQLAQELDYNLMLS
                     QQGHLTLAHAERAVNVAHERAEVNRTLGVDSRVIGPPEIGKLCPELDLSQRPAFPIMA
                     ALYHPPGGVLRHDAVVWAYGRQADRLGVQLHQNTEVTGINVDGGKVTGVRTNRGDISA
                     PIVVSAVAGWTSEITRMAGVQTPITTHPLQAFVTEPLKPFLDVILVSATLHVYISQTD
                     RGEVLIGSEIEPYSSYSQVSTLDFLEQTANHVLELLPILGRVKVMRAWGGLCDVTPDY
                     SPIMGETEVEGFLIDGGWGTYGFKATPICGVTMADLIHTGRVPDLIAPFALSRFRDRT
                     LVSEIAAAAVSH"
     gene            1309..>2001
                     /locus_tag="VIX62_05990"
     CDS             1309..>2001
                     /locus_tag="VIX62_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY5859817.1"
                     /translation="MLPLDPSAEDMRAMGTAALEEMITFIHGLDDAPADNLEGSMELA
                     RELLSPPPEEGGDFDELMEEFLAAMSKAREYAGPGYLAYIPGGGLFTSALGEFLARGY
                     NRFVGMWLQGPAAVAIEENVTRWLCDEFGFPAGSQGILTTGGSLANLSAMVTARHHRL
                     GEDFSDGVYYVSEQAHASVTKAATIAGFSRRNLRLVPTDDELRMDVDALREMVAQDRR
                     DGFRPFLVAPSAG"
BASE COUNT          345 a          665 c          656 g          335 t
ORIGIN      
        1 tgtcgagcct cgacggtcgc cccgtcgggt ggcaggcgct gcgagcggag gggtgggcct
       61 agatgcaccc ggtccgctac ttcaagccat ggcgcatgtg gcgcaggcat gacctcgcga
      121 agacctacga cgtcatcatc atcggcggtg gcgcgcacgg cctcgcgatc gcctacgaac
      181 tcgccaagcg tggcatcacc aacggcgccg tcctcgagaa ggactacatc ggtgccggtg
      241 gcagcggccg gaacaccacg atcatccggg cgaactaccg cacgcccgaa ggtgtcgcct
      301 tctacaagga agcgctccgt ctgtacgaac agctcgcgca agagctcgac tacaacctga
      361 tgctgtccca gcagggccac ctgacgctcg cgcatgcaga gcgagcggtg aacgtcgcgc
      421 acgaacgtgc tgaagtgaac cgcacgctgg gtgtcgattc ccgggtgatc ggtccgccgg
      481 agatcggcaa gctctgccct gaactcgacc tgtcgcagcg tccggcgttc ccgatcatgg
      541 cggcgctcta tcacccgccc ggcggcgtac tccgtcacga cgccgtggtg tgggcatacg
      601 gtcgtcaggc cgatcgcctg ggggtgcagc tgcaccagaa caccgaggtc accgggatca
      661 acgtcgacgg cggtaaggtc acgggcgtac gaacgaaccg tggtgatatc tcagccccga
      721 tcgtggtgtc ggcggtcgca gggtggacca gcgagatcac gcgcatggcc ggcgtgcaga
      781 cgccgatcac gacccacccg ctccaagcgt tcgtcaccga gccgctgaag ccgttcctgg
      841 acgtgatcct ggtgtcggcg accctgcatg tctacatctc ccagaccgat cgcggtgagg
      901 tgctgatcgg ctcggagatc gagccgtact cgagctactc gcaggtctcg acactcgact
      961 tcctggaaca gacggcgaac cacgtgctgg aactgctgcc gatcctcggc cgggtcaagg
     1021 tgatgcgcgc atggggcggg ctctgcgatg tcacccccga ttactcgccg atcatgggcg
     1081 agaccgaggt cgaaggcttc ctgatcgacg gcggttgggg aacctacggc ttcaaggcaa
     1141 cgccgatctg cggcgtcacg atggccgatc tgatccacac cggacgagtg cccgacctga
     1201 tcgcaccgtt cgctctctca cggttccgag atcgcacgct cgtgtcagag atcgccgccg
     1261 ccgccgtctc gcactagccg ttcagcacgc gcaggtacga tggtccgcat gctgcccctc
     1321 gacccctccg ctgaggacat gcgggccatg ggcacggccg ccctggagga gatgatcacc
     1381 ttcatccacg ggttggacga cgcgcctgcg gacaacctcg agggctccat ggaactggcc
     1441 cgcgaactcc tgtcgccgcc tccggaagaa ggcggcgact tcgacgaact gatggaggag
     1501 ttcctcgccg ccatgtcgaa agcccgcgag tacgcgggtc ctggctacct ggcgtacatc
     1561 cccggtgggg ggctgttcac ctcggcgttg ggggagttct tagcgcgtgg ttacaaccgg
     1621 ttcgtgggca tgtggttgca aggccctgcc gccgtcgcga tcgaagagaa cgtcactcgc
     1681 tggctgtgcg acgagttcgg gttcccggcg gggtcgcaag ggatccttac caccggtggc
     1741 tcgctcgcga acctgtcggc catggtgacc gctcgtcacc accggctcgg cgaggacttc
     1801 agcgacggcg tctactacgt cagcgagcaa gcccatgcca gtgtgacgaa agcggcgacg
     1861 atcgcggggt tctcacgacg gaacctccgc ctggtaccca ccgacgacga actccgcatg
     1921 gacgtcgatg cgttgcgcga gatggtcgcc caggatcgcc gcgacgggtt ccgcccgttc
     1981 ctggtggcgc catcggcggg c
//
LOCUS       DATDBZ010000243         4199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_288125, whole genome shotgun sequence.
ACCESSION   DATDBZ010000243 DATDBZ010000000
VERSION     DATDBZ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288125"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VIX62_05995"
     CDS             complement(<1..221)
                     /locus_tag="VIX62_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859818.1"
                     /translation="MTGPGLDWREFSALRPDLADAGKQLLYQFGVGLAFLGTVRADGG
                     PRLHPFCPVIDDGLFAFIVPSPKRDDLHR"
     gene            complement(218..1075)
                     /locus_tag="VIX62_06000"
     CDS             complement(218..1075)
                     /locus_tag="VIX62_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5859819.1"
                     /translation="MSARVPVKRTAKLFIGGAFPRSESGRSYEVFADDGKPLAYAAQA
                     SRKDLREAVVAARGAFPRWSGMTAYNRGQILYRVAELMEGRRSQFRDELADAGAADPD
                     RGVDAAIDRWVWYAGWADKIAQTLGTSNPVAGPYFNFTIPEPTGVVGIVAPIDQSLLG
                     LVSRLAPAIVGGNTTVVLASERSPLPAVSLAEVLATSDVPGGVVNILTGVTAELVPWL
                     ASHMDVNAIDVTGVPDELRADTDRAAADNVKRVHHAPDADPFAPEAQSPYEITALMEF
                     KTVWHPMGA"
     gene            complement(1072..2553)
                     /locus_tag="VIX62_06005"
     CDS             complement(1072..2553)
                     /locus_tag="VIX62_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019357741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5859820.1"
                     /translation="MADQIEPTGTETATWAYASAPESTDIVRLEDRYGLFINGEFAEP
                     KSGRWFTTINPATEEPLAEVAEAGTEDVAVAVEAARAATDAWQGLPGSERAKYVFRIA
                     RQLQERAREFAVLETMNGGKPIKESRDVDLPLAAAHFFYYAGWADKLGYAFPGRPTPR
                     PVGVAGQIIPWNFPMLMAAWKLAPALAAGNTVVLKPAETTPLTALLLAQVIQEAELPP
                     GVVNIVTGAGETGGALVDAPVDKIAFTGSTEVGKAIRRATAGTGKRLTLELGGKAANI
                     VFEDAPLDQAVEGVINGIFFNQGHVCCAGSRLLVQEPVFDALLTKLKDRLQTLRVGDP
                     LDKNSDVGAINSRAQLEKIEELVASGVDEGAEIYQPACDLPERGFWFRPTLFTGVSQS
                     HRIAQEEIFGPVLSILTFRTPEEAVEKANNTPYGLSAGVWTEKGSRILWMAQRLRAGV
                     VWANTFNRFDPTSPFGGYKESGFGREGGLHGLEPYLTFDGSAS"
     gene            complement(2562..3521)
                     /gene="deoC"
                     /locus_tag="VIX62_06010"
     CDS             complement(2562..3521)
                     /gene="deoC"
                     /locus_tag="VIX62_06010"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY5859821.1"
                     /translation="MAEAASIQRQDDARPAVSSSEAARIVARTTSVDAVGLEGRAAAL
                     ATRSIKRESKRWALDLVIRCMDLTTLEGSDTVGKIVALCAKAVRPDPTRPEVPSVAAV
                     CLYPQLVPVAVDQLRGTGVAVASVAGGFPSGLGPLDARLQEIRDVAAAGADEIDIVLN
                     RSLFLGGRYGEAFDELVAAREAAGSTHLKVILETGELGSYDRVRQASMLAMAAGADFI
                     KTSTGKIGVNATLPVALCMLEAVRDFRDQTGRDVGVKVAGGIRKSKQAIQHLVLVHET
                     LGDAWMRPERYRIGASSLLNDVLMQLEKERTGTYAGPDHFTID"
     gene            3608..3961
                     /locus_tag="VIX62_06015"
     CDS             3608..3961
                     /locus_tag="VIX62_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY5859822.1"
                     /translation="MDRTVSIIITLSLTLGGIACGAGASEPSACSTFTTSTSVEMDDF
                     SYGPACVQVDTGGTLALDNVGAAPHTFTVEGTEVSVDVAAGAQGSADLSGVAAGTYTV
                     LCTYHPQMKATMEVG"
     gene            3990..>4199
                     /locus_tag="VIX62_06020"
     CDS             3990..>4199
                     /locus_tag="VIX62_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859823.1"
                     /translation="MRTRTIALAALLALVAGACSLASDEPVPAPTGSAPSSVPTVGGT
                     PDATGSTGSASPHPTGDCTEAKAVDL"
BASE COUNT          628 a         1512 c         1424 g          635 t
ORIGIN      
        1 tcccggtgga gatcgtcgcg cttgggcgac gggacgatga aggcgaacag gccgtcatcg
       61 atcacggggc agaaggggtg cagccggggc ccgccgtcgg cccggaccgt gccgaggaac
      121 gcgagaccca cgccgaactg atagagcagc tgcttcccgg catcggccag gtcgggacgc
      181 aacgccgaga actcgcgcca gtccagtcct ggaccggtca cgcgcccatc gggtgccaga
      241 cggtcttgaa ctccatgagc gccgtgatct cgtaggggct ctgcgcctcg ggggcgaacg
      301 ggtcggcgtc cggggcgtga tgcacccgct tgacgttgtc cgcggcggcg cgatccgtgt
      361 cggcccgcag ctcgtcgggc acgcccgtca cgtcgatcgc gttgacgtcc atgtggctcg
      421 cgagccacgg caccagctcg gcggtgacgc cggtgaggat gttcacgacc ccgcccggga
      481 cgtcgctggt ggcgagcacc tcggcgaggg acaccgccgg gagcggcgac cgttcgctgg
      541 ccagcaccac cgtggtgttc ccgcccacga tcgccggagc aagccgcgac accagcccca
      601 gcagcgactg gtcgatcggc gccacgatcc ccaccacgcc cgtcggctcc gggatcgtga
      661 agttgaagta cgggcccgcg acggggttcg acgtcccgag cgtttgcgcg atcttgtcgg
      721 cccacccggc gtaccagacc cagcgatcga tcgcggcgtc cacccctcga tcgggatccg
      781 cggcccccgc atcggccagc tcgtcgcgga actgcgagcg gcgcccctcc atcagctcgg
      841 ccacccggta caggatctgg ccgcggttgt acgcggtcat gcccgaccat ctcgggaacg
      901 ccccgcgcgc cgccaccacg gcctcgcgca gatccttgcg ggatgcctgc gccgcgtagg
      961 ccaacggctt gccgtcgtcg gcgaacacct cgtaggagcg cccactctcg ctgcgtggga
     1021 acgcgccgcc gatgaacagc ttcgccgtgc gcttcaccgg cacccgtgcg ctcatgacgc
     1081 gctcccgtcg aacgtgaggt agggctcgag accgtgcagc ccgccctcgc ggccgaaccc
     1141 gctctccttg tagccgccga acggcgacgt gggatcgaac cggttgaacg tgttggccca
     1201 caccacgccg gcccgaaggc gctgggccat ccagaggatc cgcgaaccct tctcggtcca
     1261 gacccccgcg ctcaggccgt acggggtgtt gttcgccttc tcgacggctt cctcgggagt
     1321 gcggaacgtg aggatcgaca ggaccgggcc gaagatctcc tcctgcgcga tgcggtggga
     1381 ctgcgagacc ccggtgaaca acgtgggtcg gaaccagaac ccacgctcgg gcagatcgca
     1441 cgcgggttgg tagatctcgg cgccctcgtc gacgccgctc gccaccagct cctcgatctt
     1501 ctcgagctgg gcgcgcgagt tgatggcgcc gacgtcgctg ttcttgtcga gtgggtcgcc
     1561 cacccgaagg gtctgcagcc ggtctttgag cttggtgagc agcgcgtcga acaccggttc
     1621 ctgcacgagc agacgcgagc ccgcacaaca cacgtgcccc tggttgaaga agatgccgtt
     1681 gatcacgcct tcgaccgcct ggtccaaggg tgcgtcttcg aacacgatgt tcgcggcctt
     1741 gccgccgagc tccagcgtga gccgcttgcc ggtgccggcg gtggcgcgac ggatcgcctt
     1801 gcccacctcg gtcgagccgg tgaaggcgat cttgtccacc ggggcgtcaa cgagcgcgcc
     1861 gccggtctcc cccgcacccg tcacgatgtt caccacgccg ggcggcagct cggcttcctg
     1921 gatcacctgg gcgagcagca acgcggtgag gggtgtggtc tccgcgggct tcagcaccac
     1981 ggtgttgccg gccgcgagcg cgggcgccag cttccaggcg gccatcaaca tggggaagtt
     2041 ccacgggatg atctggccgg ccaccccgac cggacgaggc gtgggacgcc ccgggaacgc
     2101 gtacccgagc ttgtcggccc acccggcgta gtagaagaag tgcgccgccg ccagcggcag
     2161 gtcgacgtcg cgcgattcct tgatcggctt gccgccgttc atcgtctcca gcacggcgaa
     2221 ctcgcgcgca cgctcctgca gctgccgcgc gatgcggaac acgtacttcg cgcgttcgct
     2281 gccgggcagg ccctgccagg catccgtcgc cgcgcgggca gcctcgacgg cgaccgcgac
     2341 gtcctccgtc cccgcctcgg cgacctcggc gagcggctcc tccgtggccg ggttgatcgt
     2401 ggtgaaccac cgcccgctct tgggctcggc gaactcaccg ttgatgaaca ggccgtagcg
     2461 atcctcgagc cggacgatgt ccgtcgactc cggggcgctc gcgtacgccc acgtggccgt
     2521 ttcggtcccg gttggttcga tctggtcggc cacgatgctc ctcagtcgat cgtgaagtgg
     2581 tccgggccgg cataggttcc ggtccgttcc ttctcgagct gcatcaacac gtcgttcaat
     2641 aggctggaag ccccgatccg gtatcgctcg gggcgcatcc acgcgtcgcc gagcgtctca
     2701 tgcaccagca cgaggtgctg gatcgcctgc ttggacttcc ggatgccgcc cgccaccttc
     2761 accccgacgt cgcgacccgt ctggtcgcgg aagtcgcgga ccgcctcgag catgcacagg
     2821 gcgaccggca acgtcgcgtt gacaccgatc ttgccggtgg aggtcttgat gaagtccgca
     2881 ccggccgcca tcgcgagcat cgaggcctgc cgcacccggt cgtaggaccc gagctcgccg
     2941 gtctccagga tcaccttcag gtgcgtggac cccgccgcct ctcgcgcagc gacgagttcg
     3001 tcgaacgcct cgccgtagcg gccgcccagg aacagcgagc gattcagcac gatgtcgatc
     3061 tcgtcggcgc ccgccgcggc gacgtcgcgg atctcctgca gcctggcatc gagcgggccg
     3121 agcccgctcg ggaacccgcc cgccacgctg gccacggcga cgccggtgcc gcggagctga
     3181 tccacggcca ccggcaccag ctgtgggtac aggcacacgg cggccaccga cgggacctcg
     3241 ggccgcgtcg gatcaggtcg gacggccttg gcgcacagcg cgacgatctt gccgaccgtg
     3301 tcgctgccct ccagggtggt gagatccatg caccggatca ccaggtcgag ggcccaccgc
     3361 ttggactccc gcttgatcga ccgggtcgcg agtgccgcgg cgcgcccctc caatccgacg
     3421 gcatccaccg atgtggtccg ggcgacgatc cgcgccgcct cggacgagga caccgcaggg
     3481 cgggcatcgt cctgccgttg gatggaagcg gcctcggcca tgccgccatc gtagcgaaga
     3541 cgtgacgggc gctcggaggc ggggcccgta ggctgacggc ggccgaacga ggagagcgga
     3601 acgagccatg gaccggaccg tgtcgatcat catcacgctg tcgttgacct tgggcgggat
     3661 cgcctgtggg gcaggggctt ccgagccctc ggcgtgctcg acgttcacga cgtccacctc
     3721 cgtcgagatg gacgacttct cctacgggcc cgcatgcgtg caggtggaca ctggcggcac
     3781 cctggccttg gacaacgtgg gcgcggcacc gcacaccttc acggtcgaag gcaccgaggt
     3841 gagcgtggac gtcgccgccg gagcgcaggg atccgcggat ctttccggcg ttgcggcagg
     3901 cacctacacc gtgctctgta cgtatcaccc acagatgaag gccacgatgg aggtcggctg
     3961 agccggctcg acaccaccga ggaggttcga tgcgcacgag gacgatcgcc ctggccgcgc
     4021 tcctggcgtt ggtggccggc gcctgcagcc tcgccagcga cgaaccggta cccgcgccga
     4081 ccggcagcgc gccatccagc gtcccgacgg tgggcggtac ccccgatgcg accgggtcca
     4141 cgggcagtgc ctcaccgcac cccaccggcg actgcacgga agccaaggct gtcgatctc
//
LOCUS       DATDBZ010000244         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1082733, whole genome shotgun sequence.
ACCESSION   DATDBZ010000244 DATDBZ010000000
VERSION     DATDBZ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082733"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VIX62_06025"
     CDS             <1..900
                     /locus_tag="VIX62_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5859824.1"
                     /translation="RRGVVSELVQNLVDGIGRGSTYALLALGISLIFGVMHLVNFAHA
                     ELITVGAYVAYYLSTRGLGWWALAPAIVLVAVLAAMTSERLVFRWVRNASPFTMLLAS
                     FALEQLSHACWAVFVSPNKRSFPGPGWAFDTVEVAGIRFEVMDLVTIATTFVVLLATA
                     FVFRRTMFGISVRAASEDFDAARLMGVRSNQVIRGAFALSGALAGIAGIFWLMRAGQV
                     TPAAGVTPMLKGVLAALIGGMGSLRGAVIGGFALGIAEVFLRSWLPSGIVGLTDGVVF
                     LLIAVLFIFRPQGIITVPHAERV"
     gene            902..>2043
                     /locus_tag="VIX62_06030"
     CDS             902..>2043
                     /locus_tag="VIX62_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5859825.1"
                     /translation="MARPLLGNVFPDRTRDVALPAIGSLITFTVLVLGGLAAQALIGG
                     STDQLVTSMLIDAIIVLGLQIYIGNTGVLSFGHIGFGAIAGYVFAVLAIDPDKKSSVI
                     PDAPWNLDEVHLSPILATVVAVLVTLVVAVVVGLGLARSGAQSGAVAATVITLALLFV
                     TRELAVNWTELTGGDRAGLSFSVGDTLDSRIPIYVVLAVSILIARWFACSRSGRLAHA
                     AREDNLAARAVGVNPQVQQMIALLLSVAVVSVAASLRVYEIGNITPKFFFFEYTLITL
                     VMLIVGGRNSVTGALTGVVVITVAREVARRMAGDGYEFFGISLDGTVTDIVFREGLPD
                     IVLGLAMLVFMIVRPNGLLNDWELDTWIGRRLRRNREVEPAAAAPD"
BASE COUNT          264 a          697 c          689 g          393 t
ORIGIN      
        1 cggcgaggtg tcgtgagcga gctggtccag aacctcgtcg acggcatcgg gcggggcagc
       61 acgtacgccc tgctggcgct cggcatctcg ctgatcttcg gcgtcatgca cctcgtcaac
      121 ttcgcccacg cagagctgat cacggtcggt gcgtacgtcg cctactacct gtccactcgg
      181 ggcctcgggt ggtgggcgct ggctccggcg atcgtcctgg tagccgtgct cgcggcgatg
      241 acgagcgagc ggctcgtgtt ccggtgggtg cgcaacgcca gcccgttcac gatgctgctg
      301 gcgtcgttcg ccttggagca gctgtcgcat gcctgctggg ccgtcttcgt ctcaccgaac
      361 aagcgctcct tccccgggcc cggttgggcc ttcgacacgg tcgaggtcgc cggcatccgc
      421 ttcgaggtga tggacctggt caccatcgcc acgacgttcg tcgtcctcct cgccacggcg
      481 ttcgtcttcc gccgcacgat gttcgggatc tccgtccggg cggcgagcga ggacttcgac
      541 gccgcccggc tgatgggggt caggtcgaac caggtgatcc gcggcgcgtt cgctctgtcg
      601 ggagcgctgg cgggaatcgc cggcatcttc tggctcatgc gcgccggcca ggtcaccccg
      661 gcagccggcg tgactcccat gttgaagggc gtgctggcgg cgctgatcgg cgggatgggg
      721 agtctgcgcg gtgccgtgat cggcgggttc gcgctcggca tcgccgaggt cttcctgcgc
      781 tcgtggctcc cgagcgggat cgtcggcctg accgacggag ttgtgttcct cctgatcgcc
      841 gtgctcttca tcttccggcc acagggcatc atcaccgttc cccacgcgga gcgtgtctga
      901 catggcccgc cccctgctcg gcaacgtctt ccccgaccgc acccgcgacg tcgccctccc
      961 ggcgatcggt tcactgatca ccttcaccgt gctggtgctc ggtgggctcg ccgcgcaggc
     1021 actgatcggg ggatcgaccg accagctcgt cacgtcgatg ctgatcgacg ccatcatcgt
     1081 gctcggactc cagatctaca tcggcaacac gggcgtgctc tcgttcgggc acatcggatt
     1141 cggggcgatc gccggatacg tgttcgcggt gttggcgatc gatcccgaca agaagtcgag
     1201 cgtgatcccg gacgctccgt ggaacctcga cgaggtgcac ctctccccta tcctcgccac
     1261 cgtcgtcgcc gtgctcgtca cgctcgtggt ggcggtggtc gtcgggctcg ggctggcccg
     1321 ctcgggcgca cagagcggtg ccgtggcagc gaccgtgatc acgctcgccc tgctgttcgt
     1381 gacgcgcgag ctggcggtca actggaccga gctcacgggt ggtgaccggg ccggactgtc
     1441 gttcagcgtc ggcgacacgc tcgactcacg gatcccgatc tacgtcgtgc tggcggtctc
     1501 gatcctgatc gcccgctggt tcgcctgcag ccgaagcggc cgcctggcgc acgccgcccg
     1561 cgaggacaac ctggcggcgc gggcggtcgg cgtcaatccc caggtgcagc agatgatcgc
     1621 attgctgctg tcggtggcag tcgtgtcggt ggcggcatcg ttgcgcgtgt acgagatcgg
     1681 caacatcact cccaagttct tcttcttcga gtacaccctg atcacgctcg tgatgctgat
     1741 tgtcggcggg cgcaacagcg tcaccggtgc gctgaccggg gtcgtggtga tcacggtcgc
     1801 acgcgaagtg gcgcgcagga tggccggcga cggctacgag ttcttcggca tcagcctcga
     1861 cggcacggtc accgacatcg tcttccgcga gggtcttccc gacatcgtgc tcgggctcgc
     1921 catgctcgtg ttcatgatcg ttcgcccgaa cggtctcctc aacgactggg agctcgacac
     1981 ctggatcggt cgccgactgc gtcgcaaccg ggaggtcgag ccggcagcgg ccgcgcccga
     2041 cca
//
LOCUS       DATDBZ010000245         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1513758, whole genome shotgun sequence.
ACCESSION   DATDBZ010000245 DATDBZ010000000
VERSION     DATDBZ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513758"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VIX62_06035"
     CDS             complement(<1..314)
                     /locus_tag="VIX62_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY5859826.1"
                     /translation="MIARTWRGAVRAEDADAYVEYLEETGLSEYRATPGNQGVYGLRR
                     IEGDRCEFLLVTLWDDMDAVRAFAGDDPETAVFYPEDDRYLVDRDLVSNHYEVVAAPE
                     QG"
     gene            415..1464
                     /locus_tag="VIX62_06040"
     CDS             415..1464
                     /locus_tag="VIX62_06040"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEY5859827.1"
                     /translation="MRVIRSTGTPFERGTAVGSALREEIDASCAFTMTWAADLGADRR
                     RVEAMLAPYDEATMRFAPDLSEVLAGMADGSGVDPVALRATNAFEELYVVLDPEAVGE
                     PIERCTDALLEGVDGPLLVHQEQWYAADVDSVAIVIDQPDEGVGVIAPVVASGLPLVG
                     MNAFGTAMGAMSLTATDERPGIPRMIVARRALDARDREEAWRTVTTSERAGGYSWGWA
                     FADGTTAIVETTATTASDLQDATTHTNHALDGFVAAVCPTGSDGSLSRLARMRALTTG
                     RDRWTVPEASALLADHGADGQDICVHPVPAEGPESSAIMFGMVADVANRTLWVAPANP
                     CEHPFEPYALDDLLA"
     gene            1476..>2155
                     /locus_tag="VIX62_06045"
     CDS             1476..>2155
                     /locus_tag="VIX62_06045"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY5859828.1"
                     /translation="MDIRVLAAIPFRTFPQLSIGPVTIRTFGVFVAIGIVVGVWVFLR
                     YARDRDLDAELLSRLAWWVIVLGIIGSRVLFVLTHWSDFSGDPLSMFAVWQGGLQFSG
                     AFLISIVVIVWFTRRHPEMPGLVVSDGIVFGLAPGLAIGRLGCIAVGEHLGTATTFPL
                     AWKYLGGDTREPIDGGVGAVIHNTALYELFLLLPLVALLWYLQRRRVQPGWLTATFLL
                     WYGTQRFFT"
BASE COUNT          288 a          771 c          733 g          363 t
ORIGIN      
        1 tagccctgct cgggcgctgc gacgacctcg tagtggttcg agaccaggtc gcggtcaacc
       61 aggtaccgat cgtcctccgg atagaagacc gccgtctccg ggtcgtcgcc cgcgaacgcg
      121 cgcacggcgt ccatgtcgtc ccacagcgtc accagcagga actcacagcg gtccccctcg
      181 atccgtcgca gtccgtagac gccttgattg cccggcgtgg cccgatactc ggacagtccg
      241 gtctcctcca ggtactcgac gtaggcatcg gcgtcctcgg ctcgcaccgc acctcgccag
      301 gtcctcgcga tcatgcccgc ctccttgagt cgtgggaacc gttcatacca gcgcgccacg
      361 ccttgcgtcg cactcggcga cgcttcgccg gttcgggtgg gtaggcttgc cgccgtgcga
      421 gtgatccgca gcaccggcac acccttcgag cgcggcaccg ccgtgggttc cgccctgcgc
      481 gaggagatcg atgcctcgtg cgcgttcacg atgacctggg ccgccgatct gggcgccgac
      541 cgtcggcgcg tcgaagccat gctggctccc tacgacgagg ccacgatgcg gttcgctccc
      601 gacctcagcg aggtgctcgc cgggatggcc gacggctcgg gcgtggaccc ggtggcgctg
      661 cgtgccacga acgccttcga ggagctctac gtcgtgctcg atcccgaggc ggtcggcgaa
      721 cccatcgaac gctgcaccga cgcgctgctc gagggcgtcg acggtcccct cctcgtgcac
      781 caggaacagt ggtatgccgc cgatgtcgac agcgtcgcga tcgtgatcga ccaaccggac
      841 gagggcgtcg gcgtgatcgc ccccgtcgtg gcctcgggcc tgcccctggt ggggatgaac
      901 gccttcggca ccgcgatggg cgcgatgtcc ctcaccgcca ccgacgaacg gccgggcatc
      961 cctcggatga tcgtggcccg gcgcgccctc gatgcacgcg atcgcgagga ggcgtggcgc
     1021 acggtcacga cgtcggagcg agccggcggc tacagctggg gttgggcgtt cgccgacggc
     1081 accacggcga tcgtcgagac gaccgccacc acggcgagcg acctgcagga cgccaccacc
     1141 cacacgaatc acgccctcga cggcttcgtg gcggcggtgt gtcccaccgg ctccgacgga
     1201 tcgctgagcc gcctggcgag gatgcgcgcg ctcaccaccg gtcgcgatcg gtggaccgtg
     1261 ccggaggcga gcgccctgtt ggccgaccac ggagccgacg gccaggacat ctgcgtccat
     1321 cccgtcccgg cggagggccc ggagtcgagc gccatcatgt tcgggatggt ggccgacgtc
     1381 gccaaccgaa ccctgtgggt cgcgcccgcg aacccgtgtg agcacccgtt cgagccctac
     1441 gcgctcgatg acctgctggc ctgaggctcg gaccgatgga catcagggtg ctcgccgcga
     1501 tccccttccg caccttcccg cagctgtcga tcggccccgt gacgatccgc acgttcggcg
     1561 tgttcgtcgc gatcggcatc gtcgtcggtg tatgggtctt cctgcgctac gcccgggacc
     1621 gcgacctcga cgcagagctg ctgtcacgcc tggcgtggtg ggtgatcgtg ctgggcatca
     1681 tcgggtcgcg cgtgctgttc gtgctgaccc attggagcga cttctcgggc gacccgctgt
     1741 cgatgttcgc cgtctggcag ggcggcctgc agttctccgg ggcgttcctg atctccatcg
     1801 tggtgatcgt gtggttcacc cggcgccacc ccgagatgcc cggcctggtg gtgagcgacg
     1861 ggatcgtgtt cggactggcg cccggcctcg cgatcggacg gctcgggtgc atcgcggtcg
     1921 gcgagcacct cggcaccgcg acgaccttcc cgctggcgtg gaagtacctg gggggcgaca
     1981 cacgcgagcc gatcgacggc ggcgttggcg ccgtgatcca caacaccgcg ctgtacgagc
     2041 tgttcctgct gctgcccttg gtggcgctgc tctggtacct gcagcggcgc cgcgttcagc
     2101 ccggttggct cacggcgacg ttcctgctct ggtacggcac gcagcggttc ttcac
//
LOCUS       DATDBZ010000246         2681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_288591, whole genome shotgun sequence.
ACCESSION   DATDBZ010000246 DATDBZ010000000
VERSION     DATDBZ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288591"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VIX62_06050"
     CDS             <1..563
                     /locus_tag="VIX62_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEY5859829.1"
                     /translation="TSDEPFVVDEHLPGEGKVVYLSLGSLGCMDVGLMQRLIDAMAAT
                     DHRTIVSMGPLKDQMTLGPNMYGDQFLPQPSILPQCDLLITHGGNNTVCEGFHFGLPM
                     IALPLFWDQYDNAQRLRETGFGERLATYDWTQAELVDAVDRLLADDDLHARMTRDAAA
                     IQADPGRVRGADLIERVAVTGEPVLA"
     gene            560..1042
                     /locus_tag="VIX62_06055"
     CDS             560..1042
                     /locus_tag="VIX62_06055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY5859830.1"
                     /translation="MTDVAVRALTPADVDACDAIVLSLPYHFGQEVGRQQCAAAVRAE
                     AGWVCELNGLVGGFCTFVRHLDTSAEITWLAVHADHRGSGLGTRLVDHLATQMAAEGR
                     RLLLALTVSASDGPDDVEDGYDATRAFYHSAGFVEARDFAGFWGPADTPVLMVRVLDP
                     "
     gene            1039..2235
                     /locus_tag="VIX62_06060"
     CDS             1039..2235
                     /locus_tag="VIX62_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859831.1"
                     /translation="MTDVDVLDVLSLPGGAELTLRRDGHDIVIDPRDGADGSIGSGAF
                     EGLLTLLRAGEPRGSFSFLRVSDVPEAAGERPIDVDQSNRSVVVGEQVVVKVFPRTTR
                     GPQPALDLPAHLAAVGFGAMPTPFGAVTWTDGDGTPVVVATAAAYLPGARDGWEWYLE
                     LLLGWLAGGVDDAATFEPATSVGELAGALHAALATASSVLPSPVTFVEKDAILGWKMR
                     AEVALDEALALTTGEEGERLRGREGRIRAAFDVFDQVPTAAAMRIHGDLHVGQILRWD
                     GGDAVTDFDGNPLAPPEVRVASDTPVRDVASLVRSVDHMGRIAQRRDAGSIEDIERWI
                     ERSRELVVEGYLRGLDRAGHLSLFEPTLLFPLEVAQVSHEYAYAARYLPSWLRVPDLA
                     LQAIFP"
     gene            2274..>2681
                     /locus_tag="VIX62_06065"
     CDS             2274..>2681
                     /locus_tag="VIX62_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron dicitrate transport regulator FecR"
                     /protein_id="HEY5859832.1"
                     /translation="MPDPDPRRFLQDLEAKPAALRALADAIGGMWPEALDRTSTRVVV
                     GMGSSRYAGSVFATRWQQGGIGAWSELASAAAGAPPAPGILAIGVSASGTTPATVEAL
                     ARHRGTSTTVAITNHPGAALSEAADLTIPMLAGE"
BASE COUNT          408 a          890 c          933 g          450 t
ORIGIN      
        1 gcacctcgga cgaaccgttc gtcgtcgacg aacacctgcc tggtgagggc aaggtcgtct
       61 acctgtcgct cggttcgctc gggtgcatgg acgtggggct catgcagcga ttgatcgatg
      121 cgatggcggc tacggatcac cgcacgatcg tctcgatggg tcccctcaag gatcagatga
      181 cgcttggccc gaacatgtac ggcgaccagt tcctgccgca gccttcgatc ctgccccagt
      241 gcgacctgct gatcacgcac gggggcaaca acacggtgtg cgaggggttc cacttcgggc
      301 tgcccatgat cgccttgccg ctgttctggg atcagtacga caacgcccag cgcctgcggg
      361 aaactgggtt cggcgaacgt ctggccacct acgactggac ccaggccgag ttggtggatg
      421 cggtcgatcg actgctggcc gatgacgacc tgcacgcgcg aatgacccgc gatgctgcgg
      481 ccatccaggc agatccgggt cgcgtcaggg gcgccgacct gatcgaacgt gtcgccgtga
      541 ccggcgagcc cgttctggca tgaccgacgt cgctgtccgg gcgctgacac ccgccgatgt
      601 cgacgcgtgc gatgcgatcg tgctcagcct gccgtaccac ttcggtcaag aggtgggacg
      661 acagcagtgc gccgcagcgg tgcgcgctga ggcgggatgg gtctgcgagt tgaacggact
      721 ggtcgggggg ttctgcacct tcgttcgcca cctcgacacc tcagcggaga tcacgtggct
      781 ggcggtgcac gccgaccatc ggggctcggg tctggggacg cggttggtcg accatctggc
      841 gacacagatg gcggctgaag gacgccgtct gttgttggcg ctcacggtct cggcgagcga
      901 cggtcccgac gatgtggagg acggctacga cgccacgagg gcgttctacc actccgcggg
      961 gttcgtagaa gcgcgagact tcgctgggtt ctggggcccg gccgataccc cggtcctcat
     1021 ggtgcgtgtc ctggacccgt gaccgacgtc gacgtcctcg atgtgttgtc gctgccgggc
     1081 ggggccgaac tcacgttgcg tcgcgacggg cacgacatcg tgatcgatcc ccgcgacggc
     1141 gccgatggct cgatcggcag tggcgcgttc gagggactgc tgaccctgct gcgagccggt
     1201 gaaccacgcg ggtcgttctc gttcctccgg gtgagcgatg tgcccgaggc ggcgggagag
     1261 cgccccatcg acgtggacca gtccaatcgt tccgtggtgg tcggggagca ggtcgtggtc
     1321 aaggtcttcc cacggaccac ccgaggtccc cagccggcgc tcgatcttcc ggcccacctg
     1381 gccgcggtgg ggttcggtgc gatgcccact ccgttcggcg cggtcacgtg gaccgacggc
     1441 gacggcaccc cggtggtggt cgccacggca gcggcctacc taccgggagc ccgtgatgga
     1501 tgggagtggt acctcgaact cctcctgggt tggctcgcgg gcggggtcga cgatgcggcg
     1561 accttcgaac cagcgacatc ggtcggcgaa ctcgcgggtg cactccacgc ggcgttggcg
     1621 accgcctcga gcgtccttcc ttccccggtg acgttcgtcg agaaggacgc gatcctgggt
     1681 tggaagatgc gcgccgaggt cgcgttggac gaggccctgg cgctcacgac cggcgaggaa
     1741 ggggaacggc tgcgagggcg agaaggccgg atccgcgcgg cattcgatgt attcgaccag
     1801 gtcccgaccg cggcagcgat gcgcatccac ggtgatctgc atgtcggcca gatcctgcgt
     1861 tgggacggcg gagacgccgt caccgacttc gatgggaacc cgctcgcgcc ccccgaagtc
     1921 agggtggcca gcgacacgcc ggttcgtgac gtcgccagcc tggtgcgctc ggtcgatcac
     1981 atgggaagga tcgcccagcg ccgggatgcc ggttccatcg aggacatcga acgatggatc
     2041 gagcgatccc gcgagctcgt ggtcgagggg tacctccgcg gcctcgaccg cgccgggcac
     2101 ctctcgttgt tcgaacccac cttgctgttc cccctggaag tggcgcaggt gagtcacgag
     2161 tacgcctacg ccgctcgata ccttccgtca tggctgcgcg tgcccgatct cgccctgcaa
     2221 gcgatcttcc cgtagaccca tccacccagc gtcctgagcg acaggagcca cccgtgccag
     2281 acccagatcc ccgccgattc ctccaggacc tcgaggcgaa gccggctgcc cttcgagcgc
     2341 tcgccgacgc gatcgggggc atgtggcccg aagcgttgga ccgaacgtcg acccgcgtcg
     2401 tggtcggcat gggctcgtct cgctacgccg ggtcggtgtt cgccaccaga tggcagcaag
     2461 gtggcatcgg cgcgtggtcc gaactcgcat cggctgccgc aggtgcgccg cccgcacccg
     2521 ggatcctcgc gatcggcgtc agcgcgtccg gcacgacgcc ggcgaccgtg gaagcgctcg
     2581 ctcgccatcg aggaacgagc accacggtcg ccatcacgaa ccatcccgga gctgccttga
     2641 gcgaggcggc cgacctcacg atcccgatgc tcgcgggtga g
//
LOCUS       DATDBZ010000247         8408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_56709,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000247 DATDBZ010000000
VERSION     DATDBZ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56709"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VIX62_06070"
     CDS             <1..221
                     /locus_tag="VIX62_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY5859833.1"
                     /translation="TPILSPGTSITLAIGVIGPRALVVDGEVVARPACTLSCTFDHRV
                     LDGATVGRALTDLVSRLENRQALGDLPA"
     gene            218..1135
                     /locus_tag="VIX62_06075"
     CDS             218..1135
                     /locus_tag="VIX62_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY5859834.1"
                     /translation="MKIVSLLPSATEIVYALGLGDSLIGVTDECDYPPDAVTKPVVSR
                     SALPQGRPLGSREIDAAVRERMDAQQPLYVLDTDRLRREQPDVILTQDLCRVCAVPAG
                     HVEEALASLGVGDQTKVVSLDPHSLEDVIAQLETVGSLLGAEARGKELAEALRERVAA
                     VKQQAQRLPSVSVFCMDWAEPPFVAGHWVPEMVEAVGATNLLSDKGEPSHTVTHREIR
                     DALPEVLLFMPCGYYLEEAEEEGERLLTHPEIAATPAARNGNVFAVDATSFFSRPGPR
                     IVDGLEIVAWAAHPEAYPEPSPGSITRLR"
     gene            1132..2067
                     /locus_tag="VIX62_06080"
     CDS             1132..2067
                     /locus_tag="VIX62_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5859835.1"
                     /translation="MTSVEPTAGRRSVLTTTEGTHRGAFTPLDWGLFAAIGGIWGSSF
                     LFIAIGLEAFEPGLVTWLRVLFGAGVLWLVPGARKPVDRADRPRLVVISVLWVAIPFT
                     LFPLAEQHITSALTGLLNGSLPIFAAAMGALMLRRWPGRPHAIGLGVGFVGIALIALP
                     AAGQGTSEMLGVLMVLLAVVCYGVAVNVATPLTQRYGALPAMARMLALASIWTAPFGL
                     WGLSRSAFAWNAFAAVLALGALGTGLAFVLMGTLVSRVGATRGAFAIYLVPVVAMVLG
                     AVFLDEEIFAISIVGIALVLTGAFLASRKDTARAG"
     gene            2057..2866
                     /locus_tag="VIX62_06085"
     CDS             2057..2866
                     /locus_tag="VIX62_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEY5859836.1"
                     /translation="MPGDPWQPEAPILEFDPAPSAVIEPGEVIEPIDVPLHVVLCFFQ
                     DVITTVIEELEGRVIDHVVSEIGRNPIYEVELDGRRLGIVHPGVGAPLAAGFLEELVA
                     RGSRSFVACGGAGVLVPDVALGHVIVPTSAVRDEGTSYHYLPPSRTASPTPQAVDAIV
                     ETLEAHHVPFVTGATWTTDALYRETRGKVERRVAEGCLTVEMEAAAFFAVAAFRGVSF
                     GQLLYAGDDLSGDAWDQRAWNDHTTGRETLFRLAAEAVLRLPPDPAPTATG"
     gene            2896..3663
                     /locus_tag="VIX62_06090"
     CDS             2896..3663
                     /locus_tag="VIX62_06090"
                     /inference="COORDINATES: protein motif:HMM:NF025384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HEY5859837.1"
                     /translation="MAHDVTIDGLRFRVRNPLGVAGLTIITLGLYGLYWYTAANNDTR
                     MYLRNYSIRPGVSLFALILNLIGTQFIALALLLSSPWLAVGVVLVIPSFVSVFRTGRR
                     IALMQVHAGVEETSPGIALVLFLLFFLVGAGIYLQAGLNRVWAAAGSAPEPEAAPEPV
                     GVTMPGGVPSVAAAPRTDARATGHNLVDPGDVGSRVTFQFELPNGYTTEAVGVFERWD
                     EDAQTYFVRKKDGTEVRVPARGVRHGKVIPPAPQPTV"
     gene            3729..4127
                     /locus_tag="VIX62_06095"
     CDS             3729..4127
                     /locus_tag="VIX62_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEY5859838.1"
                     /translation="MSELPTALIRFRLGQEDAHYGGNLVAGATQLKVIGDLITELAMK
                     YDGDEGLFRAYESVEFLSPLYAGDWIEGRGRIVEVGNSSRKCEFEVYKIGAALTHISD
                     SAAEFLDEPVLSCKAVGTTVVPKEHQRFSH"
     gene            complement(4139..5068)
                     /locus_tag="VIX62_06100"
     CDS             complement(4139..5068)
                     /locus_tag="VIX62_06100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY5859839.1"
                     /translation="MILSPDTRAAPVMAPIIGEAFPDLATPRPRLLFTGEEFDTWAAG
                     DVVMKFPRSEDDARKLAVEVAAHPVVADRLGELVPKILGVAEPSDRFPFLSVAYICAR
                     GRQGQTIEGPIIQPKAWARTTLARELAQALTTLHTTPLKAVKAAGIARHPVALDPQVD
                     VGEDAIAWARRIAGDAVDAFLSDPLPMEARGAAAGVLCHADLKGEHLFVSEDGTRLTA
                     IVDWADAAIADPAADLAGLAIWLGPAFVREVLRSYDGPADEGTYHRAVFLARAGLLGF
                     LDGQLAGTSEPAPVAMLDAQLRAVFREEAPARR"
     gene            complement(5109..5519)
                     /locus_tag="VIX62_06105"
     CDS             complement(5109..5519)
                     /locus_tag="VIX62_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5859840.1"
                     /translation="MGRNFQQMDNMPAPGTSILVVDDEPQVVWVLQFSLEAEGYQTFA
                     ANNGVEALSEISEHRPTMMVLDIMMPTMDGWSVLEAMTELPREERPRVVVVSALASLR
                     DRAKAAELGADAYVPKPFNVDELLEVLHGLERAS"
     gene            complement(5657..6652)
                     /gene="purM"
                     /locus_tag="VIX62_06110"
     CDS             complement(5657..6652)
                     /gene="purM"
                     /locus_tag="VIX62_06110"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY5859841.1"
                     /translation="MSKAYRDAGVDIEAAAKAVTLIGDLAARARRPEVADEVGGFAGL
                     FRIGGDRMLAAATDGVGTKLEIARAAGRLDTVGIDLVAMCADDVVCTGAEPLFFLDYL
                     AVGRVVPERVAALVEGVAEGCRRAGCALLGGETAEHPGVMPDDQFDMAGFCVGVVAEA
                     DLLGPARVRDGDVLIGLASTGLHANGYSLVRKALEGYDLTETPEGLARPLVDELLEPC
                     AIDAPAVLSLARDGLLHAAAHITGGGFHENIPRALPPGMGAEIRRGAWPEPSIFSMVQ
                     ASSGAPDDDMFSTFNMGVGMVLVVDPSVAEEVLGRNDPRAFTIGRVVPGAGVSIA"
     gene            complement(6663..8072)
                     /gene="purF"
                     /locus_tag="VIX62_06115"
     CDS             complement(6663..8072)
                     /gene="purF"
                     /locus_tag="VIX62_06115"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY5859842.1"
                     /translation="MGAESPKEECGLFGVWAPGDDVARLTYFGLFAQQHRGQESAGIA
                     VSDDHTILVFKDLGLVSQVFNEATLTTLHGDLAIGHTRYSTTGSTTWDNAQPVFKTDG
                     AHTLALGHNGNLVNTHELAAMVPSGAATTDSDIVTTLLVQQMTETGGLEEACMQVLPK
                     LSGAFCFVMMDERSVFAARDPFGVRPLSIGRLPNGFVVASETCALDIVGAAFVRDVEP
                     GELIRFDDRGLHSVRFAESPRRALCVFESVYLARPDSRFEGTSVHESRREMGRILARE
                     HPVDADMVIAVPTTGHSAAQGYSEVSGLPYGDGLYKNNYVGRTFIQPSQTLRDRGVRL
                     KLNPLPDSIRGKRLVVIDDSIVRGTTTKQVVAALRESGATEVHLRITCPPLKWPCFYG
                     IDMPTRQELIGADLTVEEIGAYVAADSLGYLSLEGMVEAAGGGKDRFCRACFDGEYPI
                     EIPAQAGKFVLEDQLQLPT"
BASE COUNT         1237 a         2928 c         2940 g         1303 t
ORIGIN      
        1 ccacgccgat cctgtcgccc gggaccagca tcaccctggc gatcggcgtg atcgggccga
       61 gagcgctcgt cgtggacggc gaggtcgttg cccggccggc ctgcaccctg agctgcacgt
      121 tcgaccaccg ggtgctcgac ggcgccaccg tggggcgtgc cctcaccgac ctggtgagcc
      181 gcctggagaa tcggcaagcc ctgggagact tgccggcatg aagatcgtgt ccctgctgcc
      241 ctcggccacc gagatcgtgt acgccctcgg tctgggcgat tcgctgatcg gggtcaccga
      301 cgaatgcgac tacccgcccg acgcggtgac gaagccggtg gtctcccgca gcgcgctccc
      361 ccagggccgg ccgctggggt cgcgcgagat cgacgcggcc gtccgggaac gcatggacgc
      421 ccagcaaccg ctctacgtgc tggacaccga ccggctcagg cgagagcaac ccgatgtcat
      481 cctcacgcag gacctgtgca gggtgtgcgc ggtgccggcc gggcacgtcg aggaagccct
      541 ggcctcgctc ggtgtcggcg accagaccaa ggtggtgtcc ctggatccgc attcgctcga
      601 ggacgtcatc gcccagctcg agaccgtggg gtccctcctc ggcgccgaag cccgcggcaa
      661 ggaactcgcc gaggccttgc gcgaacgggt cgctgccgtg aagcagcagg ctcaacgtct
      721 gccctcggtg agcgtgttct gcatggactg ggcagagccc ccgttcgtgg ccggccactg
      781 ggtccccgag atggtcgaag ccgtcggtgc caccaacctc ctcagcgaca agggcgagcc
      841 ctcgcacacc gtcacgcacc gggagatccg cgatgccctg cccgaggtgc tgctgttcat
      901 gccgtgcggg tactacctgg aggaggccga ggaggagggc gaacgcctcc tgacccatcc
      961 ggagatcgcc gccacgcccg cggcgcgcaa tggcaacgtg ttcgcggtcg atgccacgtc
     1021 gttcttctcg cggccgggac cacgcatcgt ggacggcctg gagatcgtgg cctgggccgc
     1081 tcaccccgag gcctaccccg agccgtcacc gggctcgatc acccggctgc gatgacctcg
     1141 gtcgagccga cggccggccg gcgctcggtc ctcaccacca ccgagggcac ccaccggggc
     1201 gcgttcaccc cgctcgactg gggactcttc gcggcgatcg gcggcatctg ggggtcctcg
     1261 ttcctgttca tcgcgatcgg gctggaggcg ttcgaacccg gcctggtcac ctggctgcgc
     1321 gtgctgttcg gggccggcgt gctgtggctg gtgccgggcg cgaggaagcc cgtcgatcgc
     1381 gccgaccggc cgcggctggt cgtgatctcg gtgctgtggg tggccatccc cttcacgctg
     1441 ttcccgctcg ccgagcagca catcacctcg gcgctcaccg ggctgctcaa cggttcgctg
     1501 cccatcttcg ccgcggcgat gggagcgttg atgcttcgcc gttggcccgg ccgcccccat
     1561 gcgatcggcc tcggcgtggg attcgtcggg atcgccctca tcgcgctgcc ggccgcgggc
     1621 cagggcacga gcgagatgct gggcgtcctg atggtgttac tggcggtcgt ctgctacggc
     1681 gtggccgtca acgtggccac gcccctcacc cagcgctacg gcgcgctgcc ggcgatggcg
     1741 cgcatgctcg cgctcgcctc gatctggacc gcgcccttcg ggctctgggg gctcagccga
     1801 tccgcgttcg cgtggaacgc gttcgcggcg gtgctcgcgt tgggtgcgct cggcaccggc
     1861 ttggcattcg tgctgatggg caccctggtg tcgagggtgg gagcgacccg gggggcgttc
     1921 gcgatctacc tggtgccggt cgtcgcgatg gtcctgggcg ccgtgttcct ggacgaggag
     1981 atcttcgcga tctcgatcgt ggggatcgcg ctggtgctga ccggggcgtt cctggcatcg
     2041 aggaaggaca ccgcgcgtgc cgggtgatcc ctggcagccg gaggcgccga tcctggagtt
     2101 cgacccggcg cccagtgccg tgatcgaacc gggtgaggtg atcgagccga tcgatgtgcc
     2161 gctccacgtg gtgctgtgct tcttccagga cgtgatcacc accgtgatcg aagaactcga
     2221 gggccgggtg atcgaccacg tggtctccga gatcgggcgc aacccgatct acgaggtgga
     2281 gctggacgga cgacgcctgg gcatcgtgca tccgggcgtg ggtgcgccgc tcgcggccgg
     2341 gttcctcgag gagctggtgg ctcggggttc gcgctccttc gtcgcatgcg gcggcgccgg
     2401 cgtgcttgtg cccgacgtgg cgctgggcca tgtcatcgtt cccacgtccg ccgtccggga
     2461 cgagggcacc tcctaccatt acctgcctcc cagccggacc gcctccccca cgccccaggc
     2521 ggtcgacgcg atcgtcgaga ccctggaagc ccatcacgtg cccttcgtga cgggcgcgac
     2581 atggaccacc gacgccctct accgggagac ccgcgggaag gtcgagcgca gggtcgccga
     2641 aggctgcctc acggtggaga tggaggccgc ggcgttcttc gcggtcgcgg cgttccgtgg
     2701 ggtgagcttc ggccagctgc tgtacgcggg cgacgacctg tccggcgacg cgtgggacca
     2761 gcgcgcatgg aacgaccaca cgaccggtcg cgagaccctg ttccgcctgg ccgccgaggc
     2821 tgtgctgcgg ctcccgcccg accccgcccc aacggccacc ggctgagcgc ggcgcccgtt
     2881 cgatatcctc gcgccatggc ccacgacgtg acgatcgacg gtttgcgctt ccgcgtccgc
     2941 aaccctctgg gcgtggcggg cctcacgatc atcacgctcg gcctctacgg gctctattgg
     3001 tacaccgccg ccaacaacga cacccggatg tacctgcgca actactcgat ccgccccggg
     3061 gtctcgttgt tcgcgctgat cctgaacctc atcggcaccc agttcatcgc gctggcgctg
     3121 ctcctgagct cgccgtggct ggcggtgggc gtggtgctag tgatcccgtc gttcgtgtcg
     3181 gtgttccgca cgggccggcg gatcgccctg atgcaggtcc acgccggcgt cgaggagacc
     3241 tccccaggga tcgccctcgt cctgttcctg ctgttcttcc tggtcggtgc cgggatctac
     3301 ctccaggcgg ggctcaaccg cgtgtgggcg gccgcgggat cggcgcccga gcccgaagcc
     3361 gcacccgaac cggtgggggt gaccatgccc ggaggtgtgc catcggtggc ggcggcgccg
     3421 cgcaccgacg cccgcgcgac gggtcacaac ctggtcgatc ccggcgacgt gggctcccgc
     3481 gtcacgttcc agttcgagct cccgaacggg tacaccaccg aggccgtggg cgtgttcgaa
     3541 cgctgggacg aggacgccca gacgtacttc gtccgcaaga aggacggcac cgaggtccgg
     3601 gtaccggcac gcggcgtgcg ccacggcaag gtcatcccgc ccgcacccca gcccaccgtc
     3661 tgagcaccgc gcataccgac cctcgcagca caccaccgcc caggtcagcc aagaacatgg
     3721 aggccccgat gtccgaactg cccaccgcgc tcatccgttt ccggctcggc caggaggacg
     3781 cccactacgg cggcaacctg gtggccggcg ccacgcagct gaaggtgatc ggcgatctca
     3841 tcacggaact cgcgatgaag tacgacggag acgaagggct gttccgcgcc tacgagtcgg
     3901 tggagttcct gtcgccgctg tacgcgggtg actggatcga gggccgcggc aggatcgtcg
     3961 aggtcggcaa ctccagccgg aaatgcgagt tcgaggtcta caagatcggc gccgcgctca
     4021 cccacatcag cgacagcgcc gcggagttcc tggacgagcc ggtgctgtcc tgcaaggccg
     4081 tgggcaccac ggtggtgccg aaggaacacc agcggttctc gcactagtcc ggagcggact
     4141 accggcgggc gggcgcctct tcccggaaga ccgcgcgtaa ctgggcgtcg agcatcgcca
     4201 ccggagcggg ttccgaggtg ccggccagct gcccatccag gaaccccagc aggcccgccc
     4261 gcgccaggaa caccgcgcgg tggtaggtgc cttcgtccgc aggaccgtcg tagctccgga
     4321 ggacctctcg cacgaacgcc ggacccagcc agatcgcgag ccctgcgagg tcggcggccg
     4381 gatcggcgat cgccgcgtcg gcccagtcga cgatcgccgt gaggcgggtg ccgtcctcgc
     4441 tcacgaacag gtgctcgcct ttgagatcgg cgtgacacag caccccggcc gcggcgccgc
     4501 gggcctccat gggaagcggg tcggagagga aggcatcgac ggcgtcgccg gcgatccgtc
     4561 tggcccaggc gatcgcgtcc tcaccgacgt cgacctgtgg gtccagcgct accgggtgtc
     4621 gcgcgatacc cgcggctttc accgccttca gcggggtggt gtgcagggtg gtcagcgcct
     4681 gggcgagttc cctggcgagg gtggttctgg cccatgcctt cggctggatg atcgggccct
     4741 cgatcgtctg gccctgtcgc ccacgcgcac agatgtaggc gaccgacagg aacgggaacc
     4801 ggtccgaagg ttcggcgacg ccgaggatct tcgggacgag ctcaccgagc cgatcggcga
     4861 ccaccgggtg cgcggcgacc tcgaccgcga gtttgcgagc gtcgtcctcg ctcctgggga
     4921 acttcatcac gacgtcaccg gccgcccacg tgtcgaactc ctcgcccgtg aacaagagcc
     4981 gtggtcgggg cgtcgccaga tcggggaagg cctcgccgat gatgggggcc atcacggggg
     5041 cggcgcgggt gtcgggggaa aggatcaccc gccgaccgta gcagggtgga gggaaggggg
     5101 tccgcggact agctggcccg ctcgaggccg tgcaggacct cgaggagctc gtccacgttg
     5161 aacggcttcg gcacgtaggc gtcggcgccg agctcggcgg ccttcgctcg atcgcgcagc
     5221 gaggccaggg cggacaccac gacgacgcgt ggacgctcct cacgcgggag ctcggtcatc
     5281 gcttcgagca ccgaccatcc gtccatcgtg ggcatcatga tgtcgagcac catcatcgtg
     5341 ggacggtgct cggagatctc ggacagggcc tcgacaccgt tgttggcggc gaacgtctgg
     5401 tagccctcgg cctccaggct gaactgcagg acccagacga cctggggctc gtcgtcgacg
     5461 acgaggatgc tggtaccggg cgcgggcatg ttgtccatct gctggaagtt acggcccatc
     5521 tcgacgaacc ctgaatcggc cgcgcgggta tatctgttcc cgaccgttgc cccgagaacc
     5581 gtccgaaggt cggtggtgcc gggggatgcg gccctagtca gccctggtca tcccaccgag
     5641 ggtccgggcg gacccctcag gcgatcgaaa cgccggcgcc cggtacgacg cgcccgatcg
     5701 tgaaggcacg agggtcgttg cgccccagca cctcttcggc aactgacgga tcgacgacga
     5761 ggaccatccc cacgcccatg ttgaacgtgg agaacatgtc gtcgtccggg gcaccggagg
     5821 atgcctgcac catcgagaag atcgagggct caggccacgc accccggcgg atctccgcgc
     5881 ccataccggg aggcaacgcg cgagggatgt tctcgtggaa cccgccgccc gtgatgtggg
     5941 ccgcggcgtg gagcaagccg tcgcgagcca gcgacaggac cgcgggcgcg tcgatcgcgc
     6001 agggctccag cagttcgtcg acgagcggcc gcgcgagccc ctcgggagtt tccgtgaggt
     6061 cgtagccctc gagggctttg cgcaccaggg agtagccgtt ggcgtgaagg ccggtggacg
     6121 ccagcccgat cagcacgtcg ccgtcccgga cgcgagccgg ccccagcaga tcggcctcgg
     6181 ccaccacccc gacgcagaac cccgccatgt cgaactggtc gtcgggcatc acgccgggat
     6241 gttcggcggt ctcgccgccc aacagggcac agcccgcgcg tcggcatccc tcggcgacgc
     6301 cctccacgag ggcggccacc cgctcgggca cgacccggcc caccgcgagg taatccagga
     6361 agaagagcgg ttcggcgccc gtgcacacca cgtcgtcggc acacatcgcc accaggtcga
     6421 tgcccaccgt gtccagacga ccggctgccc gagcgatctc gagcttcgtg cccaccccgt
     6481 cggtggccgc ggccaacatg cggtcgcctc cgatccggaa gagccctgcg aacccgccca
     6541 cctcgtcggc cacctcggga cgccgggccc gggccgcgag gtcgccgatc aacgtgacgg
     6601 ctttggccgc agcttcgatg tccacgcccg cgtcacggta ggccttgctc atcggcctgc
     6661 gatcacgtgg gcagctggag ttgatcctcc agcacgaact tgcccgcctg cgcggggatc
     6721 tcgatcgggt actcgccgtc gaagcatgcc cggcagaacc ggtccttgcc gcccccggcg
     6781 gcttcgacca tgccctcgag ggacaggtag ccgagggagt cggccgcgac gtaggcgccg
     6841 atctcctcca cggtgaggtc ggcgccgatc agctcctggc gcgtaggcat gtcgatgccg
     6901 tagaagcacg gccatttcag cggcgggcac gtgatccgca gatgcacctc ggtggcgccg
     6961 gactcgcgca gcgccgcgac gacctgcttc gtggtcgtgc cgcgcacgat cgagtcgtcg
     7021 atcaccacga gccgcttgcc tcggatcgaa tcgggcaagg ggttgagctt gagccgcaca
     7081 cctcggtcgc gcagggtctg cgacggctgg atgaacgtgc gtcccacgta gttgttcttg
     7141 tagaggccgt ccccgtacgg gaggccgctg acctcgctgt atccctgggc ggcgctgtgt
     7201 ccggtggtgg gcacggcgat caccatgtcg gcatcgaccg ggtgctcacg cgccaggatg
     7261 cggcccatct cgcgacggga ctcgtgcacg ctcgtgccct cgaaccgtga gtccggccgc
     7321 gcgaggtaga ccgactcgaa gacgcacagc gcgcgacggg gcgactcggc gaaccgcacc
     7381 gagtgcagcc cacggtcgtc gaagcggatc agctcaccgg gttcgacgtc gcggacgaac
     7441 gccgccccga cgatgtcgag cgcgcacgtc tcggacgcca ccacgaaccc gttggggagc
     7501 cggccgatcg acaggggccg caccccgaag gggtcgcgcg ccgcgaagac gctccgctca
     7561 tccatcatca cgaagcagaa ggcaccgctc agcttcggca gcacctgcat gcaggcctct
     7621 tccagcccac cggtctccgt catctgctgc acgagcagcg tggtcacgat gtcggagtcc
     7681 gtggtggcgg cgccggacgg caccatcgcc gccagctcat gggtgttgac caggttgccg
     7741 ttgtgcccga gcgccagcgt gtgggcgcca tcggtcttga acacgggctg ggcgttgtcc
     7801 cacgtggtgg agccggtggt cgagtaccgg gtatggccga tcgcgagatc gccgtgcagc
     7861 gtggtcagcg tggcttcgtt gaagacctgg ctgacgaggc cgaggtcctt gaagaccagg
     7921 atcgtgtggt cgtcgctgac cgcgatgccg gccgattcct gcccgcgatg ctgctgggcg
     7981 aagaggccga agtaggtgag ccgcgcgacg tcgtcgcccg gagcccacac cccgaagagt
     8041 ccgcactcct ctttcggcga ttcggctccc acgaggctgt cagtttaccg gctggaggcg
     8101 tgggctgccg gggcgccagc gtggatccgc ctcatgtcgg gtgctgcgcc cagacatgac
     8161 agtgggacgg ggaacgcgaa cggccggcca cccctgggtg gccggccgta ctcgcgtcgg
     8221 cgtgatcggg agatcaggcc gtgggcagca cctgcagcaa tggcgtgaag gcaccgctcg
     8281 catccagcgt cgcgtagatg atcgtgttcg tgtgctcggc gaacgccacg ggggcgtgga
     8341 acaccgtggg tccagtgccg gcgagacgaa ccttgatcct cagcgtggtc gccgggatgt
     8401 ccagcaca
//
LOCUS       DATDBZ010000248         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_901897, whole genome shotgun sequence.
ACCESSION   DATDBZ010000248 DATDBZ010000000
VERSION     DATDBZ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901897"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VIX62_06120"
     CDS             <1..403
                     /locus_tag="VIX62_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5859843.1"
                     /translation="EQVGMAAYADRPPHHLSFGQRRRVAVATVLAMKPEILVLDEPSS
                     NLDPAGRRELAEILTSLDITMFMVTHDLPYALELCPRALVMNEGVIVADGPTQRILTD
                     RLLMEANRLELPFGFYPDRMMGQLPLPNEGT"
     gene            complement(400..846)
                     /locus_tag="VIX62_06125"
     CDS             complement(400..846)
                     /locus_tag="VIX62_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY5859844.1"
                     /translation="MERTKSGYVDVMELLRSKGLRMTPQRRAIVTEVMRTKGHISPTD
                     IARTVQGEMPGVNASTVYRTLTLLEEVGVLSHSHLETGAEYHKADEAEHVHLTCARCG
                     KDDSLSLSEAKSLQDLIQRHHGFRADLTHFAITGLCQRCAKIEAGA"
     gene            915..1811
                     /locus_tag="VIX62_06130"
     CDS             915..1811
                     /locus_tag="VIX62_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY5859845.1"
                     /translation="MTVMVTGASGPVGHALVPHLLAMDEVRAVVRRPEAAEPLRAMGA
                     KVAVGRLDDADALAEVFRRVYTVIHLVGGPNQPDEEALWDANHRSVLRALAAAREAGV
                     RRFVLVSVPGASPEASDPYLRARGLAEEAVTTSGLDHAVIRSTHLVGAGCLWFTAIVA
                     GAAATPPLVIGDGHQVVAPLALEDLAAVLAAADDREGELAGTWALEGPDVVSADALVQ
                     RLAGGEATGFRHVESTDANAALDDLLGLHVSRDTAAGFCVSSRADAPDAGPEFGITPR
                     PLDEAIRGTLDRVADATGRDPG"
     gene            1821..2597
                     /locus_tag="VIX62_06135"
     CDS             1821..2597
                     /locus_tag="VIX62_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5859846.1"
                     /translation="MDLLARLEVDDGVGVVRMDRPPANAIDLQMGLDLQAAIAEGVER
                     DDVGALVIWGGPKIFAAGADIKAMADWGPDEVRPSVDALGAACDLLEAAPTISIAAIN
                     GYALGGGMELAIAADLRLVADDAHLGQPEIQLGVIPGAGGTQRLTRLLGPGRAAELVF
                     TGRQVDAQEALTLGLAGRIEPAESLFEAAVRDARAFANGPRHALAAAKAAIGAATTDP
                     GPSGIARERALFLALFGGHDQREGMRAFVEKRPPRFGESS"
BASE COUNT          394 a          859 c          952 g          445 t
ORIGIN      
        1 cgagcaggtc gggatggcgg cgtacgccga ccggccgccg caccacctct cgttcgggca
       61 gcggcgccgg gtggcggtgg ccaccgtgct ggcgatgaag cccgagatcc tggtgctgga
      121 cgagccgtcg tcgaacctgg atccggcggg ccgccgcgag ctggccgaga tcctcacgtc
      181 gctcgacatc acgatgttca tggtgaccca cgacctgccg tacgccctcg aactgtgccc
      241 gcgcgcactg gtcatgaacg aaggcgtcat cgtcgccgac ggtccgacgc aacggatcct
      301 gacggaccga ctgttgatgg aagcgaaccg gctggagcta ccgttcgggt tctaccccga
      361 tcgcatgatg ggacagctcc ccctgccgaa cgaggggacc taggccccag cttcgatctt
      421 cgcgcagcgc tggcagagtc ccgtgatggc gaagtgggtg aggtctgcgc ggaacccgtg
      481 atgacgctgg atgagatctt gcagcgactt ggcttcgctc agcgagagcg agtcgtcctt
      541 gccgcatcgg gcgcaggtca ggtgcacgtg ttcggcttcg tctgccttgt gatattcggc
      601 gccggtctcc aggtgggagt gcgagagtac gccgacttct tccagcaacg tcagcgtccg
      661 atacacggtc gacgcgttga cgcctggcat ctccccttgc accgttcgtg cgatgtcggt
      721 gggggagatg tggcccttcg tgcgcatcac ttcggtcacg atcgcgcgtc gctggggtgt
      781 catccgcaac cccttgctac gcagcagttc catgacatcg acgtagccgc tcttggtccg
      841 ttccacggca tcgatgctag cagcatgttg caggtgcaac agaaccttgc aggtgccgtc
      901 ggtagcatcg ggtcatgacg gtcatggtga cgggggcttc gggtccggtg ggacacgcgc
      961 tcgttcctca tctgctggcc atggacgagg tgcgagcggt cgtccggcgc cccgaagcgg
     1021 ctgaaccgct gcgagcgatg ggcgccaagg tggccgtcgg aaggctcgac gatgccgacg
     1081 cgctcgcgga ggtcttccgg cgggtgtaca cggtgatcca cctggtgggt gggccgaacc
     1141 agcccgatga ggaagccctc tgggacgcga accaccgctc ggtccttcga gctctcgccg
     1201 ccgcgaggga agccggggtg cgccgtttcg ttctggtctc ggtgccgggc gcttcgcctg
     1261 aggcctccga cccgtacttg cgcgctcgag ggctcgccga ggaagccgtc accacgagcg
     1321 gcctggatca tgcggtgatc cgtagcacac acctagttgg cgcgggttgc ctctggttca
     1381 ccgcgatcgt cgccggtgcg gccgcgacac ctcccctggt gatcggggac ggccatcagg
     1441 tggtggcgcc gctcgccctg gaagatctgg ctgcggtgct ggcggctgcg gacgatcgcg
     1501 agggagagtt ggcgggaacg tgggcgctgg aaggacccga cgtcgtgtcg gctgatgcgt
     1561 tggtgcaacg gttggccggg ggtgaggcga cgggtttccg tcacgtggag agcaccgatg
     1621 cgaacgccgc cctcgatgat ctgctcgggc tccacgtgtc gcgggatacc gccgccggtt
     1681 tctgcgtctc gagccgcgcc gatgcgcccg acgcaggccc cgagttcggt atcacgccca
     1741 ggccgctcga tgaggcgatc cggggaaccc tggatcgcgt cgccgatgcg accgggcggg
     1801 accccgggta gcatccggcc atggatctgc tcgcacggct ggaggtcgac gacggcgtcg
     1861 gcgtggtgag gatggaccga cctcccgcca acgcgatcga cctgcagatg ggcctggacc
     1921 tgcaggcggc gatcgccgag ggtgtcgagc gcgatgacgt cggggcgctg gtgatctggg
     1981 gcggcccgaa gatcttcgcg gcgggtgccg acatcaaagc catggccgac tggggtccgg
     2041 acgaggttcg tcccagcgtc gacgcgctcg gcgccgcctg tgacctcctc gaagcggcgc
     2101 cgaccatctc gatcgcggcg atcaacggct acgccctagg cgggggcatg gaactcgcga
     2161 tcgccgccga cctgcggctc gtggccgacg atgcgcatct tggtcagccc gagatccagc
     2221 tcggcgtgat ccccggtgcg ggtggcaccc agcgactcac gagactgctc ggacccggga
     2281 gggccgccga actcgtgttc acgggccggc aggtggatgc acaggaagcg ctgaccctcg
     2341 gcctcgccgg acggatcgag ccagcggagt cgctcttcga ggcagccgtc cgagacgcga
     2401 gagcgttcgc gaacgggcct cggcatgcct tggccgcggc gaaggccgcg atcggcgccg
     2461 cgacgaccga tcccggtccg tcgggcatcg cccgcgagcg tgcattgttc ctggcgctgt
     2521 tcggaggcca tgaccagcgc gaggggatgc gggcgttcgt cgagaagcga cctccacggt
     2581 tcggcgagtc cagctagcca cgcagcttcg cccgggaagc tatgcaccgg tgcccagacc
     2641 cacatcggaa
//
LOCUS       DATDBZ010000249         2165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_289991, whole genome shotgun sequence.
ACCESSION   DATDBZ010000249 DATDBZ010000000
VERSION     DATDBZ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289991"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VIX62_06140"
     CDS             complement(<1..1472)
                     /locus_tag="VIX62_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HEY5859847.1"
                     /translation="MPRRHYALLFALPFLLGGRADLDGQAAQPRVRIDREYRLEATML
                     GYRGIGGSIEGIRNPTLWARTGETVRITIINGEVMVHDIALEKQPVKSQQILERGETT
                     SITFKAEQSDTYYCTLPGHRLSGMEGRLDVSDEPRTTSDEGVAPTTDQRPLNLDFETG
                     SLADWTATGDAFSIVKAAGRSPELRDAPDDRAGEYWVSSRPGGSPKRGVLTSAPFPVT
                     HPYASFLVSGGAFRTTRIELVRVEDGQRLFTVSGSDAASLRPVVVDLRAQASKMIVVR
                     LVDDETGAPTASYLQESPWAHIAFDRFRFHGERPFFPDEVTPSDSSALPPLETATHAG
                     LSAIEAARAMTVPEGFTVTLAAAEPDVVRPIAFATDDRGRLWVAEAHSYPLRAPEGEG
                     RDRILILEDTNGDGRLDRRKVFIDKLNLVSGLEVGFGGVWVGAAPYLMFIPMKAGADR
                     PSGPPKVLLDGWGYEDTHETLNTFRWGPDGWLYGTHGVFT"
     gene            1818..>2165
                     /locus_tag="VIX62_06145"
     CDS             1818..>2165
                     /locus_tag="VIX62_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859848.1"
                     /translation="MRANAIVATAVVSFWLCGPVQANKYGSVEPIANPAVIDTGPLRD
                     QPLRVREAFATRLLQCGIVDRVVQALSATKAITTINDLNTHVSVGAGGFAGFTNPSYV
                     FTVIDDGPNAASID"
BASE COUNT          334 a          740 c          757 g          334 t
ORIGIN      
        1 tgcgtgaaca cgccgtgggt gccgtacagc cagccgtccg gtccccatcg aaacgtattc
       61 agcgtctcgt gggtgtcctc gtagccccag ccgtcgagca gcacctttgg cggcccggac
      121 ggccgatcgg cgcccgcctt catcgggatg aacatgaggt acggagccgc gccgacccac
      181 accccgccga acccgacctc gaggccgctg acgagattga gcttatcgat gaacaccttg
      241 cgccggtcga gccggccgtc gccattcgtg tcctcgagga tcaggatgcg atcgcggcct
      301 tcgccctccg gggcccgcag cgggtagctg tgcgcctccg ccacccacag gcggccgcgg
      361 tcgtccgtcg cgaaggcgat cggtcggacg acgtcgggct cggcggcggc cagcgtcacg
      421 gtgaaccctt cgggcacggt catcgcgcgc gccgcctcga tggccgacag accggcatgg
      481 gtcgcggtct cgagcggcgg cagcgcgctg ctgtcggacg gcgtaacctc gtcggggaag
      541 aacggccgct cgccgtggaa gcggaaccgg tcgaacgcga tgtgcgccca ggggctctcc
      601 tgcaggtacg acgcggtcgg cgcgccggtc tcgtcgtcca cgaggcggac cacgatcatc
      661 ttgctggcct gcgcccggag atccacgacc acgggacgga ggctggccgc gtcggacccc
      721 gacacggtga agaggcgctg cccgtcctcg acccggacga gttcgatgcg cgtcgtgcgg
      781 aacgcgcccc cggacaccag gaagctggca tacgggtggg tgaccgggaa cggcgccgac
      841 gtgagcacgc cgcgcttcgg gctgccgccc gggcggctgc tgacccagta ctcaccggcc
      901 cgatcgtcgg gtgcgtcacg caattcgggg gaacggccgg ccgccttgac gatcgagaac
      961 gcgtcgcccg tcgccgtcca gtccgcgagc gaaccggtct cgaagtcgag gttcagcggg
     1021 cgctgatccg tggtgggcgc gacgccctcg tccgaggtcg tccgcggctc gtccgagacg
     1081 tccagccgcc cctccatgcc cgagagccgg tggcccggca acgtgcagta gtaggtgtcg
     1141 ctctgctccg ccttgaacgt gatcgacgtg gtctcgcccc gctcgagaat ctgctggctc
     1201 ttcaccggct gcttctcgag ggcgatgtcg tggaccatca cctctccgtt gatgatggtg
     1261 atgcggaccg tctcgcccgt gcgggcccac agcgtggggt tgcgaatccc ctcgatgctc
     1321 ccgccgatgc cccgatagcc cagcatcgtg gcctcgaggc ggtattcgcg atcgatgcgg
     1381 acgcggggct gtgccgcctg tccgtcgagg tcggcgcgcc caccgagcag gaagggcaga
     1441 gcgaagagca gagcgtagtg gcggcgtggc acgtcggttc ggcctccgag gtcggtgtgc
     1501 ggtggcgagt gtactgcctt cgggcttggc ggcacacgga cgcttcgttc agcaatcgag
     1561 cagacgcccc taggcgcctg cgcgcgatag gcggtcgacg tagctgaccg cggtgggggc
     1621 gaccggtgat ggcgcgcccg ggcgcgcgcc cgggcactga ggtcacccgc cacaccccgg
     1681 cgatccagaa gcccaacagt ttcaccattg acgacgcgat ggcgaagatg agcgaagcgg
     1741 gaggcgcggg ctgagcaacg ggcgcgagag aaacctgcta gactccacgc gctcccacaa
     1801 tcgacggagg tccagccatg cgagccaatg cgattgttgc cacggccgtg gtgtccttct
     1861 ggctctgcgg cccggtgcag gccaacaagt acggatcggt agagccgatt gccaatcccg
     1921 cggtgatcga cacgggcccg ttacgtgatc agccgttgag ggtccgcgag gccttcgcca
     1981 cacgcctcct ccagtgcggc atcgtcgatc gcgtggtgca ggcgctctcg gcaacgaagg
     2041 cgatcacgac catcaacgat ctgaacacgc acgtgtcggt gggcgccggc ggctttgccg
     2101 ggttcacgaa tccgtcctac gtcttcaccg tcatcgacga tgggccgaac gcggcgagca
     2161 tcgac
//
LOCUS       DATDBZ010000250         2063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_290169, whole genome shotgun sequence.
ACCESSION   DATDBZ010000250 DATDBZ010000000
VERSION     DATDBZ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290169"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VIX62_06150"
     CDS             <1..235
                     /locus_tag="VIX62_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY5859849.1"
                     /translation="LDVADRPGVLAEIAGDFGHNGVSIERVWQEGSGDEATLVFITHR
                     AQEGALQKTLQELRESTAVRAVSSVLRVEGEEP"
     gene            271..1338
                     /gene="thrC"
                     /locus_tag="VIX62_06155"
     CDS             271..1338
                     /gene="thrC"
                     /locus_tag="VIX62_06155"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY5859850.1"
                     /translation="MQQTSGAHQWRGVIEEYRDRLPVSEATPVVTLREGGTPLVRSDP
                     LSDETGCDVWLKYDGANPTGSFKDRGMTLAISKALEEGAKAIVCASTGNTSAAAAAYA
                     AKAGMTCAVLVPKGKIAIGKMAQTLVHGARVLEVEGNFDDSLELAKDLAAHYPVTLVN
                     SVNPYRLQGQKTCAFEIVDALGRAPDVHCVPVGNAGNISSHWIGYSEYLEDGTIVEPP
                     QLMGFQAQGAAPIVRGEPVKDPQTIATAIRIGNPASWDKAVTAAKESEGAIAAVTDRE
                     ILAAYRRVAREGLFAEPASAASVAGLLHLHAEGKLPRGATVVCILTGHGLKDPEWAIS
                     GAAHPVVIKPDPAAVAAELGL"
     gene            1361..>2063
                     /locus_tag="VIX62_06160"
     CDS             1361..>2063
                     /locus_tag="VIX62_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY5859851.1"
                     /translation="MPATSANLGPGFDCFGLALDLCNEVTIDTEAEPAVTWEGEGADE
                     LPTDGTDVVHRAMLHALEAQRVHHPDVSLPSFAMHGVNRIPLERGLGSSSAAAVAGVA
                     AASALLGEAGWDDHPFTTYAYAAELEGHPDNAAPAAFGGFTIVSSAVLRFDPHPDLRP
                     VVLVPNRVRLATADARAAVPRQIDRTDAVHNVAQAALTALALTHDPSLLTFALEDRLH
                     QRARLALVPEIEAVFD"
BASE COUNT          333 a          705 c          706 g          319 t
ORIGIN      
        1 cctcgatgtc gccgaccgcc ctggcgtcct ggccgagatc gccggcgact tcggccataa
       61 cggcgtgtcg atcgaacgcg tgtggcaaga gggctccggc gacgaggcga cattggtgtt
      121 catcacgcac cgtgctcagg aaggtgcgct gcagaagacc ctgcaggaac tccgtgagtc
      181 cacggccgtg cgcgcggtga gcagcgtgct acgggtcgaa ggagaggaac cgtgaccgcc
      241 gacggcccga tgcccggcgc ggcaactagt atgcaacaga ctagtggcgc acaccaatgg
      301 cgcggggtca tcgaggagta ccgggaccgg cttccggtgt cggaggccac gccggtggtg
      361 acccttcgtg agggcgggac gccgttggtt cggtccgacc ccctttccga cgagaccggc
      421 tgcgatgtgt ggctcaaata cgacggcgcc aaccccaccg gctcattcaa ggatcggggc
      481 atgaccctgg cgatctccaa ggcgctcgag gaaggtgcca aggccatcgt gtgcgcgtcg
      541 acgggcaaca ccagcgccgc cgcggccgcc tacgccgcga aggcgggcat gacgtgtgcg
      601 gtgctggtgc ccaaaggcaa gatcgcgatc gggaagatgg cgcagacgct ggtccacggt
      661 gctcgcgtgc tcgaggtcga gggcaacttc gacgactcgc tggaactggc gaaggatctt
      721 gcagcgcact accccgtgac gctcgtgaac tccgtgaacc cctaccggct ccaaggacag
      781 aagacgtgcg cgttcgagat cgtcgacgcg ctgggccgcg cacccgacgt gcactgcgtg
      841 ccggtgggca acgccggcaa catctcgagc cattggatcg gctactccga atacctcgag
      901 gacggcacga tcgtcgagcc gccccaactg atggggttcc aagcgcaggg cgccgccccg
      961 atcgtccgcg gcgagccggt gaaggacccc caaacgatcg ccaccgcgat ccgcatcggc
     1021 aatcccgcat cctgggacaa ggccgtgacc gcggcgaagg agtccgaagg tgcgatcgcc
     1081 gccgtcaccg atcgcgagat cctggctgct taccgccggg tggctcgtga aggattgttc
     1141 gccgagcccg ccagtgcggc cagcgtcgcc ggtctgctgc acctgcacgc cgaggggaag
     1201 ctcccacgcg gagcgacggt cgtctgcatc ctcacgggcc atggcctcaa ggatccggag
     1261 tgggcgatct cgggtgccgc ccaccccgtc gtgatcaaac ccgatcccgc ggccgtcgcc
     1321 gctgagttgg gcctgtagcg ccgtgcgtgt cacggttcgg gtgcccgcga cgtcagccaa
     1381 cctcgggccg gggttcgact gcttcgggtt ggccctcgac ctctgcaacg aggtcacgat
     1441 cgacaccgag gctgagcccg ctgtcacctg ggagggcgaa ggcgccgacg agctgcccac
     1501 cgatggcacc gacgtcgtgc acagggcgat gctgcacgcg ctcgaagccc agcgggtgca
     1561 ccaccctgac gtttccttgc cctcgttcgc gatgcatggg gtgaatcgca tcccgctcga
     1621 gcgtgggctg ggttcgtcgt cggcggccgc ggtggcgggt gtggctgcgg cgagcgcgct
     1681 gctcggcgaa gcaggttggg atgatcaccc cttcaccacc tatgcctacg ccgcagagct
     1741 cgagggccac cccgacaatg ctgcgcccgc cgctttcggc gggttcacga tcgtgtcgag
     1801 cgcggtgctt cgtttcgatc ctcatcccga cctgcggccc gtcgtgctgg tgcccaaccg
     1861 ggtcaggctc gcgacggccg atgcgcgcgc cgcggtaccg cgccagatcg accggaccga
     1921 tgcggtgcac aacgtcgcgc aggcggcgct cacggccctc gccctcaccc acgacccttc
     1981 gttgctgacg ttcgccctag aggaccgact gcaccagcga gcacgtctcg ctctggtccc
     2041 ggagatcgag gcggtgttcg acg
//
LOCUS       DATDBZ010000251         2581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2129550, whole genome shotgun sequence.
ACCESSION   DATDBZ010000251 DATDBZ010000000
VERSION     DATDBZ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129550"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VIX62_06165"
     CDS             <1..754
                     /locus_tag="VIX62_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5859852.1"
                     /translation="SFYAWGSEFYNRYVQNQFGENNEPLQRDLRQWGFQHPTGLDLPG
                     EVEGLIPDAAWGNAPEQRGTEQEPGILPFGWVPGGDILLMIGSGYATVTPLQLAQAYA
                     TLANEGKICQPHVVDRIVDDTGETVKKVSGHCGERTVPYTQDQLDYILDALTNVPKLG
                     GTAYSAFQGFPLEQYPIAGKTGTAFRGYPFQDTSWFAGVVPANDPQYVVVAMVEQAGF
                     GSDVAAPIVRKVIEGIYGIESGAPAVVTAGQD"
     gene            754..1908
                     /gene="rodA"
                     /locus_tag="VIX62_06170"
     CDS             754..1908
                     /gene="rodA"
                     /locus_tag="VIX62_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY5859853.1"
                     /translation="MADLVAGRVFAQERRPIRHVDLTLLVVTAALVVIGLFAIYSATS
                     QTLRQDGLDPLSRVNKQVATAVLGFIVLLVMMTFDYRFLKVYAGFIYGITLFLLLVLQ
                     IPGVSGGDGATSNYIDIPGVSLLQISPSEFAKVGLIVILSAMLSELRTPVPMLSDVVR
                     VLMVAGLTMGLVFINVEIGTTIVLTAITVGILVVAGTRAKHLIALAAGAIILIVLAFQ
                     LGAIKDYQVARLTAFFDSGNAAEDARYNLEQSEIAVGSGGMFGRGYLHGTQTNLDYVP
                     EQHTDFIFTVIGEEFGFFGSVFVLGLFAALLWRAIRIAYLSKDSFGTYLATGVASMFA
                     IQMFVNVGMVIGIMPITGIPLPFLSYGGTAMLVNFVAVGILLNVHMRRFK"
     gene            complement(1917..>2581)
                     /locus_tag="VIX62_06175"
     CDS             complement(1917..>2581)
                     /locus_tag="VIX62_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5859854.1"
                     /translation="LLERSARRHELISRLGHELRTPVTVILGMAQTLSKGTAPVEERG
                     QILDSLVDRAHSLARLVQRFEAAVEAGLTEWADVTQVAREVAAMHDRVIVDAAPGPAM
                     ASLNRIAARRIIEELVENGLAFSAPSTTVTIRVSAEGGIPHVRVIDRGRGIETAALDR
                     IFDPLEQGEDLNTRTHQGVGLGLTLARMSARAMDGDVVLESTSTEGSTFVWIVSGNAP
                     SR"
BASE COUNT          398 a          832 c          890 g          461 t
ORIGIN      
        1 gtcgttctac gcctggggat ctgagttcta caaccggtat gtgcagaacc agttcggtga
       61 gaacaacgag ccgctccagc gtgatcttcg gcagtggggc ttccagcacc cgacggggct
      121 cgacctcccg ggcgaggtcg aaggcctcat ccccgacgcc gcctggggca atgcgccgga
      181 gcagaggggc accgagcagg agcccgggat cttgccgttc gggtgggtgc ccggcggtga
      241 catcctgctg atgatcggct ccggttacgc gaccgtgacg ccgctccagc tggcacaggc
      301 ctatgccaca ctcgcgaatg agggaaagat ctgtcagccc catgtcgtgg acaggatcgt
      361 ggacgacacg ggggagaccg tgaagaaggt cagcggtcac tgtggggagc gaaccgtccc
      421 ctacacccag gatcagctcg actacatcct cgacgcactg accaacgtgc cgaagctggg
      481 cgggaccgcc tacagcgcgt tccagggatt cccgctcgag caatacccga tcgcgggcaa
      541 gaccgggacg gcgttccgcg gctacccgtt ccaggacacc tcgtggttcg cgggcgtggt
      601 cccggccaac gacccgcaat acgtcgtggt ggccatggtg gagcaggcag gattcggctc
      661 ggatgtggcc gcgcccatcg tgcgcaaggt gatcgaaggc atctacggca tcgagtcggg
      721 agcgccggcc gtggtcacgg cggggcagga ctgatggccg acctcgtcgc cggtcgggtc
      781 ttcgcgcagg aacgccggcc gatccgccac gtcgacctca cgctgctcgt cgtcaccgcg
      841 gcgctggtgg tgatcgggtt gttcgcgatc tactcggcca ccagccagac gctgcgccag
      901 gacgggctcg atccgttgtc ccgggtcaac aagcaggtgg ccaccgcggt gctcgggttc
      961 atcgtgctgt tggtgatgat gaccttcgac taccgcttcc tgaaggtcta cgccgggttc
     1021 atctatggga tcaccctgtt cctgctgctg gtgctgcaga tcccgggcgt gtcgggcggc
     1081 gatggtgcca ccagcaacta catcgacatc cccggcgtga gcctgttgca gatcagcccg
     1141 tcggagttcg cgaaggtcgg gttgatcgtg atcctgtcgg cgatgctgtc ggagctgcgc
     1201 acgccggtgc ctatgctctc cgacgtcgtt cgcgtgttga tggtggcggg actcacgatg
     1261 ggcctggtgt tcatcaacgt cgagatcggc accacgatcg tgctcacggc gatcacggtg
     1321 ggcatcctcg tggtggccgg tacgcgggcg aagcatctga tcgcgctggc agcgggtgcc
     1381 atcatcctga tcgtcctggc gttccagctc ggggcgatca aggactacca ggtggcccgg
     1441 ctcaccgcgt tcttcgactc cggcaacgcc gccgaggacg cgcgttacaa cctggagcaa
     1501 tccgagatcg cggtgggatc gggcggtatg ttcggccgcg gctacctgca cggcacgcag
     1561 accaacctgg actacgtgcc cgagcagcac accgacttca tcttcaccgt gatcggtgag
     1621 gagttcgggt tcttcggctc ggtgttcgtg ctggggctgt tcgcggcgct gctgtggcga
     1681 gcgatccgca tcgcgtacct gtcgaaggac tcgttcggca cctacttagc cacgggcgtg
     1741 gcgtcgatgt tcgcgatcca gatgttcgtc aacgtcggga tggtgatcgg gatcatgccg
     1801 atcaccggga tcccgttgcc gttcctcagc tacggcggca cggcgatgct ggtgaacttc
     1861 gtcgcggtgg ggatcctgct caacgtgcac atgcggcgct tcaagtaagc gcgcgctcag
     1921 cgcgacggcg cgttaccgga gacgatccac acgaaggtcg aaccctcggt gctggtcgac
     1981 tcgaggacga cgtcgccgtc catcgcgcgg gcggacatgc gcgccagcgt gaggccgagc
     2041 cccacgccct ggtgggtgcg ggtgttgagg tcctcgccct gttccaacgg gtcgaagatg
     2101 cggtcgagcg ccgccgtctc gatgcctcgg ccacggtcga tcacgcgcac gtgggggatc
     2161 ccgccttcgg ccgagacgcg gatcgtgacc gtcgtgctgg gagccgagaa cgcgaggccg
     2221 ttctcgacga gctcttcgat gatgcgacgc gccgcgatcc ggttgaggct ggccatcgcc
     2281 ggtcccggcg cggcatccac gatgacgcga tcgtgcatcg cggccacctc gcgcgccacc
     2341 tgggtgacgt cggcccactc ggtcaggccg gcctccacgg cggcctcgaa ccgctggacc
     2401 agtcgggcca ggctgtgcgc ccggtcgacc aggctgtcca ggatctgtcc gcgttcctcc
     2461 acgggggcgg tgcccttcga cagcgtctgg gccatgccca ggatcacggt gaccggggtg
     2521 cggagctcgt gaccgagccg cgagatcagt tcgtggcgcc gggcgctccg ctccagcaac
     2581 g
//
LOCUS       DATDBZ010000252        11453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_489427, whole genome shotgun sequence.
ACCESSION   DATDBZ010000252 DATDBZ010000000
VERSION     DATDBZ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489427"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..178
                     /locus_tag="VIX62_06180"
     CDS             44..178
                     /locus_tag="VIX62_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859855.1"
                     /translation="MFTRPVGRVAFAGEHTAGADHHGTMEGALRSGRRAAEQVGALLA
                     "
     gene            253..1092
                     /locus_tag="VIX62_06185"
     CDS             253..1092
                     /locus_tag="VIX62_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5859856.1"
                     /translation="MSQEAAPTEPVLLVEDIGHVRRLTMNRPKALNALNHDLTDAMSD
                     ALREAEADEHVRVIVLRGAGRAFCAGYDLTEDADEGELDAAHWHDSLKASVATMLEFT
                     DCAKPTIAQVHSYCLAGGTDLMLACDLAVAADDAKFGYVDVRFGSGVVSMFLPWVVGV
                     RAAKEMLFTGEDRIPADEALRIGLVNRVVPVDELDAATLGLADEIAKNEPFVVQTTKR
                     AVNRVWDVAGFRAAMAANVELDVMIETANLPARDEFRRITNEEGLKKAIEWRDAKFRG
                     AQG"
     gene            1089..3191
                     /locus_tag="VIX62_06190"
     CDS             1089..3191
                     /locus_tag="VIX62_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY5859857.1"
                     /translation="MSGWNLQPLLAPRSIAIVGASESPDSWAPEIERSLRHLGYDGEL
                     YPINPKYDEVWGRPCLDSIADLPKGVDLVVFVVPARVVVRMIDDCGARGVRGVMVVSS
                     GFAEAGEEGAALQEQLRDAALRHRLPILGPNVEGFVNYVDHVAPYGTTPPPHPVAGSI
                     SVISQSGTVAWAMNQLASDRGVGLRIILGVGNEAVLGLGDMFTWAAADPKTKVVTSYI
                     ETMRDVEGIGRGLEALRKARKPVLICAPEGRSEAARRSIVAHTGALAGNTGLRDAWLR
                     GHGVVLVEDPVEMFEAAVLLSHVKKLRTHGVAAALQSGGACTLFAEAAGAAGLSLPEF
                     SARTKTKLKKVLPHYASQNNPLDVTGQAAVETDMYCGALEALANDPSVGLIAFDAFPP
                     RLAGETPWADPVLEKAVELQRRTGVAFASVAMSPLAYSSEATAFTATWKQLPFLQGHR
                     ASSGAIRALIRFTSSNGRAIAERPAHPNRAKALRVLHGKSGAIDEADGAKLLAYYGVR
                     RPKERAVRTPAQAAAFARTIGFPVAVKALAPEIPHKAKLGGVKLSLRTPDDVEVAAAD
                     VLQAATRAGARAPQVLVQQMASGTEVLVGAVVDERYGAVITVRPGGAMAEAGDAVFVP
                     CPLTAKQALDYLTEQAAHCGLDPSRHDLPAAAKAVEAVARAAHDLRHRLSSLEANPLL
                     VDAHGAVAVDALAEVRPA"
     gene            3188..4036
                     /locus_tag="VIX62_06195"
     CDS             3188..4036
                     /locus_tag="VIX62_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5859858.1"
                     /translation="MIYGLAAAVGWGLADFSGAVAGRRIGSVATVIVGQVLSALFMTA
                     LLLISGEPVAPIAAIIGWVALNGLFTAIAYMTHYRALELGPVAVVSPIGATYAVVGVA
                     LAMIVLGERPDPPELIGAAITVFGVMLVSTDLRKLRAGTHGMPAGLPWAIVSAVGFGV
                     AAFLLGYAAQQIGWISGLWASRMAQVTCYIPIAIIYRKQLSQLRPGVGLWVALAAGAA
                     DILGVVTYSAGAEQGYLTLVLAASAIFPMIAVVLSIVYLHERLVPNQYVGIGFVVGGL
                     MLLGFG"
     gene            complement(4064..4477)
                     /locus_tag="VIX62_06200"
     CDS             complement(4064..4477)
                     /locus_tag="VIX62_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5859859.1"
                     /translation="MTGEPATTDWGSELTHILVVRDIATAVAFYRDVLGATVLREYGG
                     TSCVLQLFSTWLLLVTGGDPTDDKPTVTFEAPADPDRVSHSMTFRVLDCRHAHEVLSA
                     RGALFLAPLAESDWEIRGFFRDPDGHLLEISEAKG"
     gene            complement(4474..6147)
                     /gene="betA"
                     /locus_tag="VIX62_06205"
     CDS             complement(4474..6147)
                     /gene="betA"
                     /locus_tag="VIX62_06205"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEY5859860.1"
                     /translation="MASYDFVVIGGGSAGSALGNRLSADPANEVLVLEAGRPDYIWDV
                     FIHMPAALPFPIGSRFYDWKYESEPEPHMNGRKIYHARGKVLGGSSSINGMIFQRGNP
                     LDYERWGADPGMQTWDYAHCLPYFKRMESALAADRKDEFRGHAGPLVLERGPATSPLF
                     GAFFEAVQQAGYELTDDVNGYRQEGFAPFDRNIHNGRRLSAARAYLHPVKSRPNLEIQ
                     TRAFVTKILFEGTRAVGVEYVHGKGPAKRVRTGEVVLSGGAINSPQLLQLSGVGNAAE
                     LQGLGIDVVADLPGVGENLQDHLEVYVQYASKLPVSIAPGMKMWRRPLIGAQWMFLRS
                     GLGASNHFEAGGFARSNDDVDYPNLMFHFLPIAIRYDGSAPASDHGYQVHIGPMYSDA
                     RGSIKIKSTDPREHPAMTFNYLSTDQDRREWVEAIRVARGILTQPAFDPFNAGELSPG
                     PSVETDEQILEWVANDAETALHPSCTAKMGTDEMSVVDPLTMKVHGLEGLRVVDASVM
                     PYVTNGNIYAPVMMVAEKAADLILGATPLPAEPTEFYRHKKPRKRSTTK"
     gene            6313..7344
                     /locus_tag="VIX62_06210"
     CDS             6313..7344
                     /locus_tag="VIX62_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017606886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5859861.1"
                     /translation="MPEASSVTVSCRNVWKVYGPKADRIVDSPDALLPRAELLEKTGC
                     VAAVRDVSFDVAPGEVFVVMGLSGSGKSTLIRTVTRLIDATAGEVLIDGDDVLAMNEE
                     RLRDLRRHKVSMVFQHFGLLPHRRIVDNVAFGLEIQGEDKDARTKQAEEVLDTVGLGG
                     WGEHFPDELSGGMQQRVGLARALATDPEIMLFDEPFSALDPLIRRDMQDEVVRLQREL
                     KKTMIFITHDLAEALKLGDRIAIMKDGHFVQVGSPEEVVSEPADDYVADFTKEVPRAH
                     VLTARTVMQPAAGNGERYGGDITPSTIVQDLIPHFASSDLPLRVVDGDQVLGSVDRSA
                     VMAALVEEH"
     gene            7347..9446
                     /locus_tag="VIX62_06215"
     CDS             7347..9446
                     /locus_tag="VIX62_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5859862.1"
                     /translation="MATVDAPPRSSAPRVEVGAPPEIVVPPPANPWRRRLIWIGAVVV
                     ATIVAIQLANGFPEKWVVDAQRWFANVETWVIENDETSPLFVYFLTPLKNFLNAFLDG
                     VTNVLLRMTWIGVVTAAAVIGGLLAGWKMALLAGLGFLSLGVLGLWESSMETLALILV
                     AVTVALVIGIPLGIWAGRNPKVDRILRPLLDAAQTIPAYCYLILLVLFFSIGDTTALI
                     STVIFALPPAVRLTSLGLRGVPSGAMEVADSFGTTSRQRLMKVQLPMAKPSIMLGVNQ
                     TIMMALGMVVIAAVVGAGGLGQEVLQGLRNLNVGEAFVGGTAIVIMAIVLDRVTYAWS
                     KRDRRSNSLTVGGRTIKRRWVVVVAIAAILIGVMVGREVVRQQDFPERYVVELGGNTS
                     ETGPVNAVVNNVTEFVTPVTEWISDTLVTYALVPLNDLLIGVPWWYLAGAVALLGFVV
                     SRRWTLALFAFCCLAAIGIFGMWADAMDTLSQVIIAVVISVIIAIPLGVLSAKSDGFQ
                     RVLKPILDAMQTMPAFVYLVPVVVLFNVGRVPGIIAAVIYALPPCIRLTDLGIRQVPT
                     NTTEAALSYGATSSQMLRKVQLPLARPSILLGINQTIMMVLSVVVIAGLIGAGGLGLQ
                     VVFGLTKGDIGLGVVAGISIMLLALVIDRITQAMGMAPRATRGPVGTGGLGWWTRVRA
                     ITGKAADDDLDVGTTKT"
     gene            9469..10482
                     /locus_tag="VIX62_06220"
     CDS             9469..10482
                     /locus_tag="VIX62_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5859863.1"
                     /translation="MTHRPRWWSLTILTVLLAVLAAACSDVDEGSNDGETTGSDGGTT
                     TADCGSDTVNIAVNPWTGSAVNANVAKVIMEQELGCTVELVDIDEFAQFPALSTGDLD
                     ATLEVWPSGHAKDYKKYIDGGQGVIDGGELGVIGKIGWYIPTYMLDEHPELATWEGLN
                     DNAELFATAETSPKGQMLDGDPSFVSYDAEIVENLGLNYEVVVAGTETAELAQLKKAY
                     AANEPLLFYFYTPHWANQKYDLTAVELPAYTEECGQAAADETGEYACEYPEDVLYKAF
                     NEDLQTKAPAAFDFLSAMSYDNAAQEAIALSIDVDGMDPADAAQAWVDENTDVWQAWL
                     PSS"
     gene            10555..>11453
                     /locus_tag="VIX62_06225"
     CDS             10555..>11453
                     /locus_tag="VIX62_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5859864.1"
                     /translation="MSEHVKMFIGGAWVDAEDGATFDATSPSTGAVIGSVPQGSRADA
                     QRAIDAANAAAPAWAGLSAFDRAAAMRRIASVIDERRDDLAHTLALDQGKPLVAEARD
                     EVEELIAYFDMAAADATRMEGLMPPSVDANKRVLLYRVPRGVVSIISPWNWPYTMPGE
                     ILAPAIAYGNAVVWAPAPSTSVCAAAMAAVLEAAELPPGVVNMVTGPGAVVGDEIAVN
                     PGTQAVGFIGSIATGETVASRAAGKDLLLEMGGNGPLVILEDADVGAAVEATLAACFL
                     NAGQSCTAGERILVHEAVREEFVT"
BASE COUNT         1724 a         3722 c         4150 g         1857 t
ORIGIN      
        1 gtcctactcc gcgtgggacg ccgtgtcggt ggcccgacgc gacgtgttca cgcgaccggt
       61 cggccgggtg gccttcgcgg gagaacacac cgcgggcgcc gatcaccacg gcacgatgga
      121 gggtgcgctt cgcagtggcc gccgcgcggc cgaacaggtc ggcgccctgc tcgcctagat
      181 ccggcgcgag cgcacccgcg ccgttgctag catcccggcc tcacgtcgac cgaccggaga
      241 agagggatac ccgtgagtca ggaagccgca cccaccgagc ccgtgctgct ggtcgaggac
      301 atcggccacg tccggcgtct gaccatgaat cggccgaagg ccctcaacgc gctgaaccac
      361 gatctcactg acgcgatgtc ggacgccctg cgcgaggccg aggccgacga gcacgtccgg
      421 gtgatcgtgc tgcgaggcgc aggtcgcgcg ttctgcgccg gctacgacct caccgaggat
      481 gccgacgaag gggaactcga cgcggcgcac tggcacgaca gcctgaaggc gtccgtggcc
      541 acgatgctgg agttcaccga ctgcgccaag cccacgatcg cccaggtgca ctcctactgc
      601 ctggccggcg gcaccgacct gatgttggcg tgcgatctcg cggtcgcggc cgacgatgcg
      661 aagttcggct acgtggatgt gcggttcggc tcgggcgtcg tgtcgatgtt ccttccatgg
      721 gtggtcggtg ttcgagcggc caaggagatg ctcttcacgg gcgaggatcg catcccggcc
      781 gacgaggcct tgcggatcgg cctggtgaac cgggtagtgc ccgtcgacga gctcgatgcc
      841 gccaccctgg gcctggccga tgagatcgcc aagaacgagc ccttcgtcgt ccagaccacg
      901 aagcgcgcgg tgaaccgcgt gtgggatgtc gcagggttcc gggcggcgat ggcggccaac
      961 gtcgagctcg acgtgatgat cgagacggcc aacctgcccg cgcgagatga gttccgccgc
     1021 atcacgaacg aggaaggtct caagaaggcg atcgagtggc gggacgcgaa gttccgtggt
     1081 gcccagggat gagcggctgg aacctccagc cactgctggc gccgcggtcg atcgcgatcg
     1141 tgggcgcgtc ggaatcaccc gacagctggg cgcccgagat cgaacgatcg ctccgtcacc
     1201 tcggctacga cggcgagctc tacccgatca accccaagta cgacgaggtc tggggccggc
     1261 cgtgcctcga ttcgatcgcc gacctgccga agggcgtcga cctcgtggtc ttcgtggtgc
     1321 cggcgagggt cgtggtgcgc atgatcgacg actgcggggc acgcggtgtc cgcggcgtga
     1381 tggtcgtctc gtccgggttc gccgaggccg gtgaagaagg tgccgccctc caggagcagc
     1441 tgcgcgacgc ggccctgcgc caccggctcc cgatcctcgg gccgaacgtg gagggattcg
     1501 tcaactacgt tgaccatgtc gcgccgtacg gcacgacgcc gccgccccac ccggtcgccg
     1561 gttcgatcag cgtgatcagc cagtcgggca ccgtggcatg ggcgatgaac cagctcgcgt
     1621 cggatcgtgg cgtcggcctg cgcatcatcc tgggcgtggg caacgaggcc gtgctgggtc
     1681 tcggcgacat gttcacctgg gccgccgccg accccaagac gaaggtcgtc accagctaca
     1741 tcgagacgat gcgcgatgtc gagggcatcg gccgaggtct ggaggcgctc cggaaggccc
     1801 gcaagcccgt gctgatctgc gcgcccgagg gacgcagcga agcggcacgt cgttcgatcg
     1861 tggcgcacac cggggcgctc gccggcaaca ccggcctgcg tgatgcctgg ctgcgcgggc
     1921 acggcgtcgt gctggtcgag gatccggtcg agatgttcga agcggcggtg ctgctgagcc
     1981 acgtgaagaa gctccggaca cacggcgtgg cggcggcgct gcagtcgggc ggcgcctgca
     2041 ccctgttcgc cgaggccgcg ggggcggcgg ggctgtcgct gccggagttc tccgcgagga
     2101 cgaagacgaa gctcaagaag gtgttgccgc actacgcctc gcagaacaac ccgctcgacg
     2161 tcacggggca ggccgcggtg gagaccgaca tgtactgcgg cgcgctcgag gcgctggcga
     2221 acgacccgtc ggtaggcttg atcgcgttcg atgcgttccc tcctcgactc gcgggcgaga
     2281 ccccgtgggc ggatccggtc ctggagaagg ccgtcgagct ccagcgcagg accggggtgg
     2341 cgttcgcgag cgtggcgatg agtccgctcg cctacagctc cgaggcgacc gcgttcaccg
     2401 cgacgtggaa gcagctgccg ttcctgcagg ggcaccgtgc ctcgagcggg gcgatccgcg
     2461 cgctgatccg gttcacgtcc tcgaacggac gggcgatcgc cgaacgcccc gcccacccca
     2521 accgcgcgaa ggcgctccgg gtgctccacg ggaagtcggg cgcgatcgat gaggcggatg
     2581 gcgcgaagct gctcgcctac tacggcgtcc gccgtccgaa ggagcgggcc gtgcgcacgc
     2641 ccgcacaggc ggcggcgttc gcccgcacca tcgggttccc ggtggcggtg aaggcgctgg
     2701 cgccggagat cccgcacaag gccaagctcg gcggcgtgaa gctcagcctg cgcacgccgg
     2761 acgatgtcga ggtcgcggcc gccgacgtgc tccaggccgc gacacgggcc ggcgccagag
     2821 ccccgcaggt actggtgcag cagatggcga gcggcacgga ggtcctggtg ggggcggtgg
     2881 tggacgagcg gtacggcgcc gtgatcacgg tgcgtcccgg tggtgccatg gccgaggccg
     2941 gcgacgccgt gttcgtcccg tgtcccctca cggcgaagca ggcgctggac tacctcaccg
     3001 agcaggcggc ccactgcggg ctcgacccga gccgtcacga cctgccggcg gcagccaagg
     3061 cggtcgaggc cgtagcacgg gcggcgcacg atcttcggca ccggctgtcc tcgctcgagg
     3121 ccaacccgtt gctggtggat gcacacgggg cggtcgccgt ggatgccctc gcggaagttc
     3181 gtccggcgtg atctacggcc tggccgcggc ggtcggctgg ggcctggccg acttcagcgg
     3241 cgcggtcgcg ggccgcagga tcgggagcgt cgccacggtg atcgtgggtc aggtgctgtc
     3301 ggcgctgttc atgacggcgc tgttgctgat cagcggcgaa ccggtggcgc cgatcgcggc
     3361 gatcatcggg tgggtcgccc tcaacggctt gttcaccgcg atcgcgtaca tgacccacta
     3421 ccgcgccctc gagctcggcc ccgtcgccgt ggtctcgccg atcggggcca cctatgcggt
     3481 ggtcggcgtg gcgctggcga tgatcgtgct gggcgaacgc ccggacccgc cggaactgat
     3541 cggtgcggcg atcaccgtgt tcggggtgat gctggtctcg accgatctgc ggaagctccg
     3601 cgccgggacc cacggcatgc cggccggact gccctgggcg atcgtgtcgg ccgtgggctt
     3661 cggcgtcgcg gcgttcctgc tgggatacgc cgcgcagcag atcggctgga tctcggggct
     3721 gtgggcgtca cggatggccc aggtgacctg ctacatcccc atcgcgatca tctatcgcaa
     3781 gcagctgtcg cagctgcgcc ccggcgtggg gttgtgggtg gcgctcgcgg ccggcgccgc
     3841 cgacatcctc ggcgttgtga cgtactcggc cggcgccgag cagggctacc tcacgctcgt
     3901 gctcgccgcg agcgcgatct tcccgatgat cgccgtggtg ctttcgatcg tctacctcca
     3961 cgaacggttg gtgcccaatc agtacgtggg catcgggttc gtggtgggcg ggctgatgct
     4021 gctggggttc ggctgatccc tggcgcgtcg ggctccgacg aactcagccc ttcgcttcgc
     4081 tgatctccaa caggtgcccg tcggggtcgc ggaagaaccc gcggatctcc cagtcggatt
     4141 cggcgagcgg ggcgaggaac aaggccccgc gggcggagag cacctcgtgg gcatggcggc
     4201 aatcaaggac ccgaaaggtc atggagtggc tcacgcgatc gggatcggcg ggcgcctcga
     4261 aggtcacggt gggcttgtcg tcggtggggt cgccgcccgt caccagcagg agccacgtgc
     4321 tgaagagctg cagcacgcac gacgtcccgc cgtactcgcg gagcaccgtg gcgccgagga
     4381 catcgcggta gaacgcgacg gcggttgcga tgtcgcgcac caccaggatg tgggtgagtt
     4441 cgctgcccca gtccgtggtg gcgggttcgc cggtcatttc gtcgtcgacc gtttccgtgg
     4501 cttcttgtgc cggtagaact cggtgggttc tgccggcagc ggtgtggcac ccaggatgag
     4561 gtccgcggcc ttctccgcca ccatcatcac cggcgcgtag atgttgccgt tcgtgacgta
     4621 gggcatgacc gaagcatcga cgacgcgcaa cccctcgagc ccgtgcacct tcatcgtgag
     4681 gggatcgacc accgacatct cgtcggtgcc catcttcgcg gtgcacgacg ggtgcagcgc
     4741 ggtctcggcg tcgttcgcca cccactccag gatctgctcg tcggtctcca cgctggggcc
     4801 gggtgagagt tcgccggcgt tgaacggatc gaaggccggc tgggtgagga tgccccgcgc
     4861 cactcggatc gcttccaccc attcccggcg gtcctggtcg gtcgagaggt agttgaacgt
     4921 catcgcgggg tgctcgcggg ggtccgtgct cttgatcttg atgctgccgc gcgcgtcgga
     4981 gtacatgggg ccgatgtgca cctggtagcc gtgatcgctg gccggcgccg agccgtcgta
     5041 gcggatcgcg atgggcagga aatggaacat caggttgggg tagtcgacgt cgtcgttgct
     5101 gcgcgcgaac ccgcccgctt cgaagtggtt cgacgctccc agtcctgaac gcaggaacat
     5161 ccactgcgca ccgatgaggg gccggcgcca catcttcatg cccggcgcga tcgagaccgg
     5221 gagcttgctg gcgtactgca cgtagacctc gaggtggtcc tggaggttct cgcccacccc
     5281 ggggaggtcg gccaccacgt cgatgccgag gccctgcagc tcggcggcat tgccgacgcc
     5341 cgagagctgc aggagctgcg gggagttgat cgccccgccg ctcagcacca cctcgccggt
     5401 gcgaacccgt ttggcggggc ctttgccgtg cacgtactcg acgccgacgg cccgcgtgcc
     5461 ctcgaacagg atcttcgtga cgaaggcgcg ggtctggatc tcgaggttgg gccgggactt
     5521 caccggatgc aggtaggcgc gcgccgcgga gagccgtcgg ccgttgtgga tgttgcgatc
     5581 gaagggcgcg aacccctcct ggcggtagcc gttgacgtca tcggtcagtt cgtagcccgc
     5641 ctgctgcacc gcctcgaaga aggcgccgaa tagcgggctc gtggccgggc cgcgttccag
     5701 caccaagggc ccggcgtggc cgcggaactc gtccttgcgg tcggcggcca gggcggactc
     5761 catgcgcttg aagtacggca ggcagtgcgc gtagtcccac gtctgcatgc ccgggtcggc
     5821 gccccagcgt tcgtagtcca gcgggttgcc gcgttggaag atcatgccgt tgatgctgct
     5881 ggagccgccc aggaccttgc cgcgggcgtg gtagatcttg cgcccgttca tgtgcggctc
     5941 gggctcggat tcgtacttcc agtcgtagaa gcggctgccg atcgggaacg ggagcgccgc
     6001 aggcatgtgg atgaagacgt cccagatgta gtccggacgc ccggcctcga gcaccaggac
     6061 ctcgttcgca gggtcggcgc tgaggcggtt gccgagggcg ctgcccgccg agcctccgcc
     6121 gatgaccacg aagtcatagc tcgccatggg cgcgagcatg ccgcaagtcg gcgggcggcg
     6181 catccgcgag gttgcccatg ccgcaaccct tgacttgcgc caggtcgctc ggaggccgac
     6241 tatccgaggg ttcgtaggtc gtgaggggga cgggctccgt cacggggtcc gaccggatcg
     6301 gggggtacgc ccttgccaga ggcgtcgagc gtcacggttt cctgtcgcaa cgtgtggaag
     6361 gtctacggac cgaaggccga tcgcatcgtg gattcgcccg atgccctgct cccgcgagcc
     6421 gagctgctcg agaagaccgg ttgcgtcgcg gccgtccgtg atgtctcgtt cgacgttgcg
     6481 cccggcgagg tgttcgtcgt catggggctg tcgggcagcg gcaagtccac cctgatccgc
     6541 accgtgaccc ggctgatcga cgccacggcc ggcgaggtgt tgatcgacgg cgacgacgtg
     6601 ctcgcgatga acgaggagcg gttgcgcgac ctccgccggc acaaggtcag catggtcttc
     6661 caacacttcg gcctgctgcc gcaccgtcgc atcgtcgaca acgtggcgtt cgggctcgag
     6721 atccagggcg aggacaagga cgcgcgcacg aagcaggccg aggaggtcct ggacaccgtt
     6781 ggcctcggtg gatggggcga gcatttcccc gacgagctct cgggcgggat gcagcagcgc
     6841 gtcggcttgg cgcgcgcgct cgccaccgac ccggagatca tgctgttcga cgagccgttc
     6901 agcgcgctgg acccgttgat ccgtcgcgac atgcaggacg aggtggtgcg cctgcagcgc
     6961 gagctcaaga agacgatgat cttcatcacc cacgacctgg cggaagccct gaagctgggg
     7021 gaccgcatcg cgatcatgaa ggacggccat ttcgtccagg tgggctcgcc cgaggaagtc
     7081 gtctccgagc cggcggacga ctacgtggcc gacttcacga aggaagtacc gcgggcgcac
     7141 gtgctgaccg cccggaccgt gatgcagccg gccgccggca acggtgagcg ctacggcggc
     7201 gacatcacgc cgtcgacgat cgtgcaggac ctgatcccgc acttcgcttc ctccgacctc
     7261 ccgctgcgcg tggtggacgg ggaccaggtg ctgggttcgg tcgatcgcag cgcggtgatg
     7321 gccgccttgg tcgaggagca ctgagcgtgg cgacggttga cgcaccgccg cgatcgtcgg
     7381 cgccacgcgt cgaagtcggt gccccacccg agatcgtcgt cccgccaccg gcgaacccgt
     7441 ggcgtcgtcg tctcatctgg atcggcgccg tcgtggttgc gacgatcgtt gcgatccaac
     7501 tcgcgaacgg attcccggag aagtgggtgg tcgatgcgca gcgatggttc gccaacgtgg
     7561 agacctgggt gatcgagaac gacgagacga gcccgctgtt cgtctacttc ctgaccccgc
     7621 tgaagaactt cctcaacgcg ttcctcgatg gtgtgacgaa cgtcctgctg cggatgacct
     7681 ggatcggcgt cgtgaccgcc gccgccgtga tcggggggtt gctcgccggc tggaagatgg
     7741 cgctgctcgc tgggttgggg ttcctgtcct tgggcgtgtt gggtctttgg gagtcgagca
     7801 tggagaccct ggcgctgatc ctggtggccg tcaccgtggc gctcgtgatc gggatcccgc
     7861 tcgggatctg ggcgggacgc aaccccaagg tcgaccggat ccttcgcccg cttctggatg
     7921 cggcgcagac catcccggct tactgctatc tgatcctgct ggtgctgttc ttcagcatcg
     7981 gcgacaccac ggcgttgatc tccaccgtga tcttcgcgct gccgccggcg gttcgcctca
     8041 ccagcctcgg gctgcgcggc gtgccctcgg gggcgatgga ggtggccgat tcattcggga
     8101 cgacctcgcg gcaacgcctc atgaaggtgc agctccccat ggcgaagccc tcgatcatgc
     8161 tgggcgtcaa ccagacgatc atgatggcgc tgggcatggt cgtgatcgcc gccgtggtgg
     8221 gcgccggcgg gctcggtcag gaggtcctcc agggactgcg aaacctcaac gtcggcgagg
     8281 cgttcgtggg aggcaccgcg atcgtgatca tggcgatcgt gctcgaccgc gtgacctacg
     8341 cctggagcaa gcgcgaccgc cgctcgaact cgttgacggt cggtggccgg acgatcaagc
     8401 gccggtgggt ggtcgtcgtg gcgatcgccg cgatcctcat cggggtcatg gtcggccgcg
     8461 aggtcgtgcg acagcaggac ttccccgaac gctacgtcgt cgagctcggc ggcaacacct
     8521 cggagacggg gccggtcaac gccgtcgtga acaacgtcac cgagttcgtc acgccggtca
     8581 ccgagtggat tagcgacacg ctggtcacat acgccctggt ccccctgaac gatctgttga
     8641 tcggggtgcc gtggtggtac ctcgcgggcg cggtggcgct cctggggttc gtcgtgtccc
     8701 ggcgctggac gttggcgctg ttcgcgttct gttgcctcgc ggcgatcggc atcttcggca
     8761 tgtgggccga cgcgatggac acgctgagcc aggtgatcat cgcggtggtg atctcggtga
     8821 tcatcgcgat cccgctcggg gtgttgtcgg ccaagagcga tgggttccag cgtgtgctca
     8881 agcccatctt ggacgcgatg cagacgatgc cggcgttcgt ctacctggtg ccggtggtgg
     8941 tgctgttcaa cgtgggccgt gtgccgggca tcatcgccgc ggtgatctac gcgttgccgc
     9001 cgtgcatccg cctcacggat ctcgggatcc ggcaggtgcc cacgaacacc acggaggccg
     9061 cgctgtctta tggggcgacc tcgtcgcaga tgctgcgcaa ggtgcaactg ccgctggcgc
     9121 gtccgtcgat cctgctgggg atcaaccaga cgatcatgat ggtgttgtcc gtcgtcgtga
     9181 tcgcggggct gatcggcgcc ggtggcctgg gtctgcaagt cgtgttcggc ctgacgaagg
     9241 gcgatatcgg gctcggcgtc gtggctggga tcagcatcat gctgctcgcc ttggtgatcg
     9301 accggatcac gcaggcgatg gggatggctc cgcgcgcgac gcgtggaccg gtcgggaccg
     9361 gcggactcgg ctggtggacg cgcgtgcgcg cgatcaccgg caaggccgcg gatgacgatc
     9421 tcgacgtagg tacgaccaag acgtagttcc aactcgaagg gaggaagcat gacgcaccga
     9481 cccaggtggt ggtcactcac catcctgacg gtgctgctcg ccgtgctcgc cgctgcgtgc
     9541 agcgatgtgg atgagggcag caacgacgga gagaccacag ggagcgacgg cggtacgacg
     9601 acggccgact gcggcagcga cacggtgaac atcgccgtga acccgtggac gggctcggcg
     9661 gtcaacgcca acgtggccaa ggtcatcatg gagcaggagc tggggtgcac ggtcgagctc
     9721 gtcgacatcg acgagttcgc gcagttcccg gccctgtcca ccggcgacct cgacgccacg
     9781 ctcgaggtgt ggccgtccgg tcacgcgaag gactacaaga agtacatcga cggtggccaa
     9841 ggggtcatcg acggtggcga gctcggtgtg atcggcaaga tcgggtggta catcccgacc
     9901 tacatgctcg acgagcaccc cgagctggcg acctgggaag ggctcaacga caacgccgag
     9961 ctcttcgcga cggccgagac ctcgcccaag ggccagatgc tggacggcga cccgagcttc
    10021 gtgtcctacg acgccgagat cgtcgagaac ctcgggctca actacgaggt cgtggtggcg
    10081 ggcaccgaga cggcggagct ggcgcagctc aagaaggcct acgccgccaa cgagccgctg
    10141 ctgttctact tctacacgcc gcactgggcg aaccagaagt acgacctgac ggcggtcgag
    10201 ctgcccgcct acaccgagga atgcggtcag gccgcagccg acgagaccgg cgagtacgcc
    10261 tgcgagtacc cggaggacgt cctgtacaag gcgttcaacg aggacctgca gaccaaggcg
    10321 ccggccgcct tcgacttcct gtcggcgatg agctacgaca acgccgcaca ggaagcgatc
    10381 gcgctgtcga tcgatgtcga cggcatggac ccggccgacg cggcgcaggc gtgggtcgac
    10441 gagaacaccg acgtctggca ggcctggctg ccctcgtcgt aagcagcgca cccgaaccag
    10501 gttggtgacg agggcggccc ggacgggccg ccctccgtca tccatggagg ggacatgagc
    10561 gagcacgtga agatgttcat cggcggcgcg tgggtcgatg ccgaggacgg cgccaccttc
    10621 gatgccacga gcccgtcgac cggcgccgtg atcggttcgg taccccaggg ctcgcgcgcc
    10681 gacgcgcaac gggcgatcga tgcggccaac gccgcggctc cggcgtgggc ggggctcagc
    10741 gccttcgatc gcgccgcagc gatgcgccgg atcgcttcgg tgatcgacga gcgccgggac
    10801 gacctcgcgc acacgctcgc gctcgatcag ggcaagccgc tcgtggccga ggcgcgcgac
    10861 gaggtcgaag agctgatcgc ctacttcgat atggcggccg ccgacgccac ccgcatggag
    10921 ggcttgatgc cgccgtcggt cgacgcgaac aagcgcgtgt tgctctaccg cgtgccgcgc
    10981 ggcgtcgtct cgatcatcag cccgtggaac tggccctaca cgatgccggg ggagatcctg
    11041 gcgccggcga tcgcctacgg gaacgccgtg gtgtgggcgc ccgcgcccag caccagtgtg
    11101 tgtgccgccg cgatggccgc ggtgctggag gctgccgagc tcccgcccgg ggtggtcaac
    11161 atggtgaccg ggccgggcgc cgtcgtcggg gacgagatcg ccgtgaaccc gggcacgcag
    11221 gcggtggggt tcatcggatc gatcgccacc ggggagacgg tggcctcgcg cgccgcgggc
    11281 aaggacctgc tcctggagat gggtggcaac ggcccgctcg tgatcctcga ggacgccgac
    11341 gtcggcgcgg ccgtcgaggc cacgctggcg gcgtgcttcc tcaacgccgg gcagagctgc
    11401 acggcgggcg agcgcatcct ggtgcacgag gcggtccgcg aggagttcgt cac
//
LOCUS       DATDBZ010000253         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1715686, whole genome shotgun sequence.
ACCESSION   DATDBZ010000253 DATDBZ010000000
VERSION     DATDBZ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715686"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="VIX62_06230"
     CDS             complement(<1..1799)
                     /locus_tag="VIX62_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamine-synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HEY5859865.1"
                     /translation="MLSPEILGRTFADAPDPDLARIAFSRVGDQPEARELLAREDILP
                     VAARLLGFSTAAADLLVRHPGEAAALADVSARSRVRLDTELAAEVERRGTADGLRVFR
                     RRAMMRIAARDLGDASLDEIVAEISHVAEACLEVAVREAAEGARIAVIGLGKLGGAEL
                     NYASDVDLLFLHEDVEAEQAERIVARLIALLAEPTADGIALRVDPTLRPGGRSGSLSL
                     TLDSTLEYYESRSATWERQAMIKARGVAGDPWLGSAFVEGVTPFVYPAHLAPSAIDEV
                     RRTKVRLEEYIRQRGKELTEVKRGRGGIRDVEFAVQLLQIVHGRRDPVLREPNTLRAL
                     TALTQQGFVATDDAEDLAEAYRFLRTLEHRLQIVRDLQTHDLPADPRARTTLARSLGM
                     ADAAELQRTYDATTAEVRTIHERLFYRPLLEAFAGPAVPSPGVDRAATEELLGGLGFA
                     SPARSYEVLRRLVDPATRIGKVLTNVFPVMVPQLALAPDPDGALVRLERIADAVGDGH
                     GPADILAGDPAAARRLAAVAGASSFSTDLLATDPGRMVALTDVGVGRGSPRATDARGD
                     LVGAVARAATGELLSHQTGAALTEVAERVVREA"
     gene            1886..>2297
                     /locus_tag="VIX62_06235"
     CDS             1886..>2297
                     /locus_tag="VIX62_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEY5859866.1"
                     /translation="MVLSAEAEYVLRTVEERGIRFVRLWFTDVLGFLKSFTITTQELE
                     GALSEGMGFDGSSIEGFSRIQESDMVAIPDPATFQVIPWKLEAPVARMFCDVWNPDGT
                     PFDGDPRGVLKRQLSRAAELGFTFYVGPELEFFYF"
BASE COUNT          351 a          787 c          814 g          345 t
ORIGIN      
        1 atggcctcgc ggacgacacg ctcggcgacc tcggtgagcg cggcgccggt ctggtgcgag
       61 agcagttcgc cggtggcggc gcgggcgacg gcaccgacca ggtcgccgcg ggcgtcggtg
      121 gcccggggcg agccccggcc cacgccgacg tcggtgaggg cgaccatcct gccgggatcg
      181 gtggcgagca gatcggtgga gaacgagctg gcgccggcga cggcggcgag gcgcctggcc
      241 gcggcagggt cgcccgcgag gatgtcggcg ggtccgtgcc cgtcccccac ggcgtcggcg
      301 atgcgctcca accgcaccag agcaccgtcg gggtcgggag ccaaggccag ctgtggcacc
      361 atcaccggga agacgttggt gaggaccttg ccaatacggg tcgcagggtc caccaggcgc
      421 ctgagcactt cgtacgaccg ggccggcgat gcgaacccca gaccgccgag cagttcctcg
      481 gtggcggcgc ggtcgacacc cggcgagggc accgccggtc ctgcgaacgc ttccagcagc
      541 ggtcggtaga agagacgctc gtggatcgtg cgaacctcgg cggtggtggc gtcgtaggtg
      601 cgctgcagct cagcggcgtc ggccatgccg agcgagcggg ccagcgtcgt ccgcgcgcgg
      661 ggatcggcgg gcaggtcgtg cgtctgcagg tcacgaacga tctgcagacg atgttccagc
      721 gtccgtagga accggtacgc ctcggccaag tcctccgcat cgtcggtggc cacgaagcct
      781 tgctgggtca gcgcggtcag ggcgcgcagc gtgttgggtt cccgcagcac agggtcgcga
      841 cggccgtgca cgatctggag caattgcacc gcgaactcca cgtcgcggat accgccgcgg
      901 ccccgtttga cctcggtgag ctccttgccg cgctgacgga tgtattcctc caggcgaacc
      961 ttggtgcgcc gaacctcgtc gatcgcactg ggtgccaggt gcgccgggta cacgaacggc
     1021 gtgaccccct cgacgaaggc cgagcccagc cacgggtcgc ccgccacacc tctcgccttg
     1081 atcatggcct ggcgctccca ggtcgccgag cgagattcgt agtactccag ggtggagtcg
     1141 agggtgagag acagcgagcc gctccggcct ccggggcgca acgtggggtc gacgcgcagc
     1201 gcgatgccgt cggcggtggg ttcggcgagg agcgcgatga gtcgggccac gatgcgttcg
     1261 gcttgctccg cctccacgtc ctcgtgcagg aacaacaggt cgacgtcgct ggcgtagttg
     1321 agctcggcgc cgcccaactt ccccagtccg atcacggcga tccgcgcacc ctcggcagcc
     1381 tcacgcaccg ccacttccag gcacgcttcg gcgacgtgag agatctcggc gacgatctca
     1441 tcgagcgagg catccccgag gtcacgcgca gcgatccgca tcatcgcccg ccggcggaac
     1501 acgcgcagtc cgtccgcggt gccgcggcgt tcgacctcgg ccgcgagttc ggtgtcgagt
     1561 cgtacacgcg atcgggcgga gacgtccgcg agggcggccg cctcaccggg gtggcgcacc
     1621 aacaggtcgg cggccgccgt cgagaagccc agcaaccggg ccgccaccgg caggatatcc
     1681 tcgcgagcga gcagctcacg agcttccggc tggtccccaa cgcgcgagaa cgcgatgcga
     1741 gccagatcgg gatcgggcgc gtcggcgaag gttcgaccga ggatctcggg actcagcacg
     1801 caggcatcat cgcatcgcct cccgctcgcc gtagagttca cacgggcgta acggttgcgg
     1861 cgcaccgccc gcctaccctt gggcgatggt cctttccgcc gaagccgagt acgtcctgcg
     1921 cacggtcgag gaacggggca tccggttcgt ccgcctctgg ttcaccgacg tcctcgggtt
     1981 cctgaagtcg ttcacgatca ccacgcagga actggaaggc gcgctttctg agggcatggg
     2041 cttcgacggc tcgtccatcg agggcttcag tcgcatccag gaatccgaca tggtcgcgat
     2101 ccccgatccc gccacgttcc aggtgatccc ttggaagttg gaagcgcccg tcgcgcgcat
     2161 gttctgcgac gtgtggaacc ccgacggaac cccgttcgac ggtgatccac gaggggtgct
     2221 caagcgccag ctgtcgcggg ccgctgagct cgggttcacg ttctacgtcg gaccggagct
     2281 ggagttcttc tacttcc
//
LOCUS       DATDBZ010000254         3034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_902742, whole genome shotgun sequence.
ACCESSION   DATDBZ010000254 DATDBZ010000000
VERSION     DATDBZ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902742"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..2142
                     /locus_tag="VIX62_06240"
     CDS             73..2142
                     /locus_tag="VIX62_06240"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEY5859867.1"
                     /translation="MADHHDQHQHDQHAGHSVALFRDKFWWSLLLSVPVLIWSPMLQD
                     LLDFSAPRFPGARWIPMAFGTVVFAYGGWPFLQGGLREFRDRQPGMMLLISLAIGVAF
                     ISSIASEAGWLDLEFWWELVLLIDVMLLGHWQEMRALGRASGALDALAALIPDVAERV
                     EDDGLHEIAVRDLRPGDLVLVRPGARVPGDGVVTQGQAGVDESMITGESRSVERNVGD
                     HVVAGTVATDSSLRVWVEAVGDDTAIAGIRRLVADAQASKSHAQALADRAAALLFYVA
                     LGSAVITFVVWAGLGRMDDAIERTVTVLVIACPHALGLAIPLVIAISTTISARAGILV
                     KDRLALERMRSVDTVLFDKTGTLTRGEPVVSGVASTAGHPDAVLRYAAAVEADSEHPL
                     ARAVVAAGERLGETTEATDFRSMTGRGVQAAVDGVAVAVGGPALLRERSLVEPPELAD
                     AVEGWRSRGASILFIVRDNSVIGAIALEDEVRPESRQAVEGLHADGVHVVIVTGDARQ
                     VADAVAADIGVDEVKAEVLPEDKEAAVAELEGRGLRVAMVGDGINDAPALARADVGIA
                     IGAGTDVAIESAGVVLASDDPRGVVGVRTLSKASYRTMQQNLVWAAGYNIVAIPLAAG
                     ALAWAGVVLSPAVGAILMSASTIVVALNAQLLRRIDLRPAPVDDPGTGSDVSSSRLGA
                     GTPPARA"
     gene            complement(2096..2902)
                     /locus_tag="VIX62_06245"
     CDS             complement(2096..2902)
                     /locus_tag="VIX62_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5859868.1"
                     /translation="MYRVELAKAFHRWRTWLLAAAIGGIPVVIVFALKVSPPAPGGAE
                     DAPPFLFQIASNGLFAALTALAVVQPFFLPLATGLFAGDAIAGEAQSGTLRYLLLRPV
                     RRSRLVLAKYASSMTLLGFLVAVTIVTGVVSGAVVFGLGPMPTLSGSTLSVAGSLLRI
                     GGSGIYMLAALSGIACIGLFISTKTDSGPGAAVATIVIAIASQILDQIPSLRVIHGFL
                     PTHGWLAFTGLFRFPVDWAPMRGGLAVSAGYTLVFLTLALVGFRRRDVMS"
BASE COUNT          499 a         1004 c         1060 g          471 t
ORIGIN      
        1 cccacctgca gcagttcacc cagctgtccg aggagatcat caaagcagtc ggctgaggaa
       61 ggtcgccgcc gcatggcgga ccatcacgat caacaccagc acgaccagca cgctggtcat
      121 agcgtcgcgc tgttccgcga caagttctgg tggagcctgc tgctgtcggt tcccgtcctg
      181 atctggtcgc cgatgctgca agacctgctg gacttctccg cgccccggtt cccgggggca
      241 cgctggatcc cgatggcatt cggcaccgtg gtgttcgcct acggcgggtg gccgttcctg
      301 caaggggggc tccgcgagtt ccgagatcga cagccaggca tgatgctgct gatctcgttg
      361 gcgatcggcg tcgcgttcat ctccagtatc gccagcgaag cagggtggct cgacctggag
      421 ttctggtggg agctggtcct gttgatcgac gtgatgctcc tgggtcactg gcaggagatg
      481 agagcgctag gccgggcttc cggcgcgttg gacgccctcg cagccctgat ccccgacgtc
      541 gctgagcggg tcgaggacga cgggctgcat gagatcgcgg tgcgcgatct tcggcccggc
      601 gatctggtcc tggtccgccc cggcgcccgg gtccccggag atggcgtggt gacgcagggt
      661 caggccggag tggacgagtc gatgatcacc ggcgaatcgc gatcggtcga gcgaaacgtg
      721 ggcgaccacg tcgtggcggg cacggtcgcc accgattcct cgcttcgcgt gtgggtcgag
      781 gccgtcggag acgacaccgc gatcgccggc atccgtcgcc tggtggccga tgcacaggcg
      841 tccaagagcc acgcccaagc actcgccgac cgagccgccg ccttgctgtt ttacgtcgcg
      901 ttgggatccg cggtcatcac gttcgtggtc tgggcggggc tcgggaggat ggacgatgcg
      961 atcgaacgca cggtgacggt gctggtcatc gcttgccccc acgcgctggg gttggccatc
     1021 ccactggtga tcgccatctc gaccaccatc tcggcaaggg cgggcatcct ggtgaaggat
     1081 cggctggcct tggaacggat gcgctcggtc gacacggtcc tgttcgacaa gaccggcacc
     1141 ctgacccgcg gcgaacccgt cgtgagcggt gtggcttcca ctgccggcca cccggacgcg
     1201 gtgcttcgat atgccgcagc agtagaagcc gacagtgagc atccgctcgc ccgagcggtc
     1261 gtggcggccg gcgaacggct cggcgaaacc acggaggcga cggacttccg ttccatgacg
     1321 gggcgcgggg tccaggccgc agtcgatggt gtggcggtag cggttggcgg ccctgcgctc
     1381 ttgcgagaac gatcactcgt cgagcctcct gagctggccg atgcggtcga gggctggcga
     1441 agcaggggtg cctcgatcct gttcatcgtt cgcgacaact cggtcatcgg cgctatcgcg
     1501 ctggaggacg aagtccgccc cgagtcccgc caagcagtgg aagggctcca cgccgacggt
     1561 gtccacgtcg tgatcgtcac cggtgatgcg aggcaggtcg cagatgcggt cgctgccgac
     1621 atcggggtgg acgaggtgaa agctgaggtc ctgccggagg acaaagaggc tgccgtggcc
     1681 gaactcgagg gccgagggct gcgcgtcgcc atggtcggcg acggcatcaa cgacgcgccg
     1741 gcgctggccc gagccgatgt cgggatcgcg atcggtgcgg gcactgacgt cgcgatcgag
     1801 tctgccggtg tggtgctggc gagcgatgat ccgcgtggcg tcgtgggggt gcggaccctc
     1861 tcgaaggcga gctatcgcac gatgcagcag aacctggtgt gggctgcggg ctacaacatc
     1921 gtcgcgatcc cgctggcggc cggggcgttg gcatgggccg gcgtggttct gtcccctgcg
     1981 gtgggcgcca tcctcatgag cgcatcgacg atcgtggtgg cgctgaacgc ccagctcctt
     2041 cgcaggatcg accttcgccc ggcgccggtg gacgatcccg gcaccggttc cgacgtcagc
     2101 tcatcacgtc tcggcgccgg aaccccacca gcgcgagcgt gaggaacacc agggtgtacc
     2161 cggcgctgac cgccaggccc ccgcgcatcg gggcccagtc gaccgggaag cggaacagcc
     2221 ccgtgaacgc gagccatccg tgggtcggca agaacccgtg gatcacgcgc aggctgggga
     2281 tctggtccag gatctggctc gcgatcgcga tcacgatcgt cgcgaccgct gcccccggcc
     2341 ccgaatcggt cttcgtagag atgaacaggc cgatgcacgc gatccccgac agtgccgcga
     2401 gcatgtagat ccccgaaccc ccgatccgca ggagcgatcc agcaaccgac aacgtggagc
     2461 ccgacagcgt cggcatcgga cccaatccga acacgacggc gcccgacacc acgccggtca
     2521 cgatcgtgac ggcgaccagg aaccccagca gcgtcatcga cgaggcgtac ttcgccagca
     2581 ccagccggga tcggcggacc ggtcgcagca gcaggtagcg cagcgtgccc gattgcgcct
     2641 cgccggcgat cgcgtcaccc gcgaacagtc cggtcgcaag cggaaggaag aatggctgga
     2701 cgaccgccaa cgccgtcagc gcggcgaaca gaccgttgga cgcgatctgg aacaagaacg
     2761 gcggggcgtc ttcggcccca cccggcgccg gtggcgacac cttcaacgcg aacacgatga
     2821 cgaccgggat gcctccgatg gcggcggcca gcaaccaggt gcgccagcgg tggaacgcct
     2881 tcgccaactc aacgcggtac atcggcgccc tccaccagcg tcatgaagac gtcttccagc
     2941 gacgatcgct cgggggcgag ttcgctgacg ccgatgcccg ctgcaaccag cgccgcattg
     3001 agggccgccg tggaggctcc ttcccccacc gcga
//
LOCUS       DATDBZ010000255         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2130036, whole genome shotgun sequence.
ACCESSION   DATDBZ010000255 DATDBZ010000000
VERSION     DATDBZ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130036"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VIX62_06250"
     CDS             complement(<1..1041)
                     /locus_tag="VIX62_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5859869.1"
                     /translation="MAERIQRSSSPTSKNQGTEGVLGFLGRDMAVDLGTANTLVYVRG
                     RGVVLNEPSVVAINTLTGAILAVGAEAKRMIGRTPSHIQAVRPLKDGVIADFDVTEKM
                     LRYFIQKVHKRRLLAKPRVVVCVPSGITGVEQRAVEEATIAAGSRRAYIIEEPMAAAI
                     GAGLPIHEPTGNMVVDIGGGTTEVAVISLGGIVTSTSIRIGGDELDEAIIQHVKKEYS
                     LLLGERTSEAIKLAIASVFPMPEELVAEIKGRDLVSGLPKTITITAEEIRRAIEEPVN
                     AIIDAIKTTLDRTPPELSADIMDRGIVLTGGGALLRGLDKRLRHETGMPVTIADTPLQ
                     AVVLGSGKCLEEF"
     gene            1195..2121
                     /locus_tag="VIX62_06255"
     CDS             1195..2121
                     /locus_tag="VIX62_06255"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY5859870.1"
                     /translation="MAFAVVFPGQGSQFEGMADPWNTHPAGRTVLAEASEALERDVVE
                     GCHDPEALATTEFVQPALLACDVAAFRVLEAEGLASSQIVGAAGHSLGEFAAFVASGA
                     MLLPEALGLVVIRGVAMQRAGEQRPGTMTALLGMDTERANELCDQVRGDDVLVVANEN
                     SSVQVVISGSVAAIERAEALASERKIRAVRLNVAGAFHSSLMGSAVEPILGVLEDIEI
                     RAPRFPIAENVSGTLVSDPGELRALLGRHVVSSVRWQACAVALQEAGASTFLEAGPGE
                     VLTKMAKRVVPGATAVAVGSPEAAAAAVSSAP"
BASE COUNT          334 a          701 c          789 g          389 t
ORIGIN      
        1 gaactcctcc aggcacttgc cggagccgag cacgacggcc tgcagcgggg tgtccgcgat
       61 ggtgaccggc atgccggtct catggcgaag tcgcttgtcc aggccgcgca ggagcgcgcc
      121 accgcctgtg aggacgatgc cgcgatccat gatgtcggcg gacagctcgg ggggtgttcg
      181 gtcgagcgtg gtcttgatgg cgtcgatgat ggcgttgacc ggttcctcga tcgcccggcg
      241 gatctcctcc gcggtgatcg tgatggtctt gggcagacca ctgacgaggt cccggccctt
      301 gatctcggcc acgagttcct cgggcatggg gaacacgctg gcgatggcga gtttgatggc
      361 ctcggaggtt cgctcgccca acagcagcga gtactccttc ttgacgtgct ggatgatggc
      421 ctcgtccagc tcgtcgccac cgatgcggat cgacgtgctc gtgacgatcc cgccgagcga
      481 gatcaccgcg acctccgtcg taccaccacc gatgtcgacc accatgttgc cggtgggctc
      541 gtggatcggg agcccggcgc cgatcgcggc cgccatgggc tcctcgatga tgtaggcgcg
      601 gcggctcccc gccgcgatcg tggcttcttc gaccgcccgc tgctcgacgc cggtgatgcc
      661 cgaagggacg cagacgacca cacgcggctt cgcgagcagg cgacgcttgt gcaccttctg
      721 gatgaagtac cgcagcatct tctccgtgac gtcgaagtcg gcgatgacgc cgtccttcag
      781 cgggcggacg gcctggatgt ggctgggcgt gcggccgatc atgcgcttgg cctcggcacc
      841 cacggcaagg atggctccag taagggtgtt gatcgcgacg acgcttggct cgttcagtac
      901 gacaccgcgt ccacgcacgt acaccagcgt gttcgcggta ccgagatcga cggccatgtc
      961 gcgaccaagg aacccgagta caccctccgt cccctggttc ttggacgtgg gagaagaaga
     1021 acgttgaatc ctctcggcca tgatgggact cgtaggccat tcaacaggag gcgctccacc
     1081 ctggcaactt cgggaggccg aaaccacggg cccgacacaa ggttcggccc cgcccgatag
     1141 gcttcgtcgg gtcgacggtg ggcagtcggt tcacgaggtg gagggggtcg ttgagtggcg
     1201 ttcgcggtgg tcttcccggg ccagggttcg cagttcgagg gcatggccga tccatggaac
     1261 acccacccgg cgggcagaac cgtgttggcc gaggcctctg aggcgctcga acgcgacgtg
     1321 gtggagggat gccacgatcc ggaggcgctc gcgaccacgg agttcgtgca acccgccctg
     1381 ctcgcctgcg acgtggcggc cttccgagtg ttggaagccg aaggattggc gtcctcgcag
     1441 atcgtcggcg ctgcaggcca ttcgctgggt gagttcgctg ccttcgtggc gtcgggcgcc
     1501 atgctgctgc ccgaagcctt gggtctggtg gtgatccgag gtgtggcgat gcagcgggca
     1561 ggtgaacagc gaccgggcac catgacggcg ctgctcggca tggacaccga gcgggccaac
     1621 gaactctgcg accaggtccg cggcgacgac gtcctggtgg tcgcgaacga gaactcttcc
     1681 gtccaggtcg tgatcagtgg atcggtcgcg gcgatcgagc gcgccgaggc cctcgcctca
     1741 gaacggaaga tccgcgccgt gcgattgaac gtcgcgggag ccttccactc ttctttgatg
     1801 ggatcggcgg tcgaaccgat cctgggtgtg ctcgaggaca tcgagatccg ggcgccgcgg
     1861 ttcccgatcg cggagaacgt gagcggcacg ttggtgagcg atcccggtga attgcgcgcg
     1921 ctgctggggc gacacgtggt gtcgtccgtc cgctggcagg catgcgccgt cgcgttgcag
     1981 gaggcgggcg cgtcgacctt cctggaggcc gggccgggcg aggtgctcac caagatggcg
     2041 aagcgggtgg ttccgggcgc gacggcggtc gccgtgggct ctcccgaggc cgccgctgcc
     2101 gcggtatcct cggcgccgtg acccggtacg ccaccatcgc cggtgtgggc tcggcgcttc
     2161 cgcctcggct cgtgcccaac agctggttcg agaccatcgt cgagacgaac gac
//
LOCUS       DATDBZ010000256         2312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1085311, whole genome shotgun sequence.
ACCESSION   DATDBZ010000256 DATDBZ010000000
VERSION     DATDBZ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085311"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VIX62_06260"
     CDS             <1..400
                     /locus_tag="VIX62_06260"
                     /inference="COORDINATES: protein motif:HMM:NF023134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEY5859871.1"
                     /translation="EWALSIRDALRTKVGENAGAPRDHAAVEILNSAAQDTGLQLCFG
                     CDENRLHTEVDGVRGVIGRILGTAFLADMDGSFHRLRECQDATCPTVFYDRSKNHTAK
                     WCSMASCGNRNKVRKFRERERAGAATDPAP"
     gene            397..1341
                     /locus_tag="VIX62_06265"
     CDS             397..1341
                     /locus_tag="VIX62_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HEY5859872.1"
                     /translation="MTTLELPLVGPAGEPVDLARTIDSHGFSDLDPMRLDAAAYTLEL
                     TVSVEATQHGSRSTPQPRRIRISPGPGRTAHLEILGPRIGARAQAQVVAAARHVLRLD
                     QDLSGFYDATAGDPDLAWVASGAGRMLQSPTAFEDVIKTVCTTNCSWSATRRMVNALV
                     SELGTPALGGDGSSTNAFPTADSMANAPERFYRDEVRAGYRGVYLIELARRVADGEVD
                     LDALAASSPADLSDDDLERTLIELPGIGPYAAAHIMMTMGRNSRLILDSWTRPTYAKL
                     LGRSKPVADATIQRRFRRYGDHAGLAFWLFLTRDWVQD"
     gene            1545..>2312
                     /locus_tag="VIX62_06270"
     CDS             1545..>2312
                     /locus_tag="VIX62_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY5859873.1"
                     /translation="MASTRHASRLVTRPARRLYRSWRAERRTLRQGLVALTLSTAASF
                     VAGLTLASITGTLSALPGLLVLIPAAVGMRGTIFGAIGARLGTASAAGTFESNLKRGG
                     VLAQNVEVAFITSFASSLWLAALAKLATNAFGEPSISFWDLVTISVVGGFLGSVLILI
                     VTLGMSVVSYRRGYDLDSVATPMVTALADMGTLPLLYLATFLVRNDTVNALVAGLCVL
                     VCVLAVVRAVRTPDMHVRRIVLEMTAVIALTPMLDIAA"
BASE COUNT          368 a          789 c          751 g          404 t
ORIGIN      
        1 tgaatgggcg ctgtcgatcc gcgatgccct gcgaaccaag gtgggtgaga acgccggcgc
       61 acccagggat catgccgcgg tcgagatcct gaactccgca gctcaggaca cagggctgca
      121 gctgtgtttc gggtgcgacg agaaccgcct gcacacagag gtcgacggcg ttcgtggtgt
      181 catcggcagg atcttgggca cggccttcct cgccgacatg gacgggtcgt tccatcgact
      241 tcgggaatgc caggacgcca cgtgcccgac ggtgttctac gaccgctcga agaaccacac
      301 cgcgaaatgg tgctccatgg cgtcgtgcgg caaccggaac aaggtgcgca agttccgcga
      361 gcgagaacgt gcgggcgccg ccacggatcc cgcgccgtga ccacgctcga actcccgctg
      421 gtgggtcccg ctggagagcc cgtcgacctg gcccgcacga tcgactccca cggcttctca
      481 gacctcgatc cgatgcgact cgacgccgcc gcctacacgc tcgagctgac ggtgtccgtc
      541 gaggcgactc aacacgggag ccgaagcacc ccccagcctc gccgtatccg gatctcaccg
      601 ggtcccggac gaacggcgca cctggagatc ctcggacctc ggatcggcgc ccgagcccaa
      661 gcgcaggtgg tcgcagctgc tcgtcacgtg ctccgactcg accaagacct ttcgggcttc
      721 tacgacgcga cggccggcga tccggacctc gcatgggtcg cgagcggtgc gggtcgcatg
      781 ctccagagtc cgacggcgtt cgaggatgtc atcaagacgg tctgcaccac caactgctcg
      841 tggagcgcca ctcggcggat ggtgaacgcc ttggtgagcg aactcggcac gcccgcgctc
      901 ggcggcgacg gatcctcgac caacgcgttc ccgaccgccg actccatggc gaacgcgcct
      961 gaacgcttct atcgggatga ggtccgtgct gggtatcggg gtgtctacct catcgaactc
     1021 gcccgtcgcg tcgccgacgg cgaggtggac ctggacgcgc tggcggcgtc gtcgccggcc
     1081 gacctttccg atgacgacct cgaacggacg ttgatcgaac tccccggtat cggtccatac
     1141 gcggccgcgc acatcatgat gacgatgggc aggaattcgc gcctgatcct ggattcctgg
     1201 acccgcccga cgtacgcgaa gttgttgggt cgatccaagc ccgtcgccga cgccacgatc
     1261 cagcggcgct tccggcggta cggcgaccac gcgggattgg cgttctggct gttcctcaca
     1321 cgcgattggg tccaggactg accccgggat cgggaggaag ctcacgaacg gctcaggaac
     1381 acctccactg gaaccgatac cttcccgggg ttgccccgcc tggtgggcga gggcatactg
     1441 cctgaggccc cgaaccggac gatcctgttg gggtcctagc gggtcccaca cgtcggaccc
     1501 aacctgcggc aacgaggagc gagtgaggaa atgatggtgc cgttatggcg tccacacgcc
     1561 acgcctcgag gttggtcacc cgaccagcac gccggctgta ccgttcttgg cgagccgagc
     1621 gtcgcacgct ccgccagggg ctggtcgcgc tcaccctcag cacggcagca tccttcgtcg
     1681 cagggctgac gctcgcctcc atcaccggca ccctctcagc cctgccgggt ctgctggtcc
     1741 tgatccccgc cgctgtcggc atgcgcggca ccatcttcgg cgcgatcggt gctcgactcg
     1801 ggacggcctc ggccgcgggt acgttcgagt ccaacctcaa gcgcggcggt gtgctggccc
     1861 agaacgtcga ggtggcgttc atcacgtcgt tcgcatcatc cctgtggttg gcagctctcg
     1921 cgaagctggc gaccaacgcg ttcggcgagc cgtcgatctc gttctgggat ctggtcacga
     1981 tctcggtggt gggcggattc ctcgggtcag tcttgatctt gatcgtcacc ctggggatgt
     2041 cggtggtgtc gtaccggcgg gggtatgacc tggactcggt cgccacgccc atggtcacgg
     2101 cgttggccga catgggaacc ctgccgctgc tctacctcgc aaccttcctg gtgcgcaacg
     2161 acacggtcaa cgcgcttgtg gctgggctct gcgtcctggt gtgcgtgctc gccgtggtca
     2221 gagccgttcg cacacccgac atgcacgtgc gccggatcgt gctggagatg accgcggtca
     2281 tcgctttaac gccgatgctc gacatcgccg cc
//
LOCUS       DATDBZ010000257         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1924207, whole genome shotgun sequence.
ACCESSION   DATDBZ010000257 DATDBZ010000000
VERSION     DATDBZ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924207"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1029
                     /locus_tag="VIX62_06275"
     CDS             64..1029
                     /locus_tag="VIX62_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5859874.1"
                     /translation="MEYRRLGRTALRVSPLCLGTMNFGLQTEEADSHAIMDRALELGI
                     NFFDTANRYGGSLGAGGTEEIIGRWFALGGGRREKVVLATKAFGAMSEWPNDGGLSAR
                     HIRRACEESLRRLQTDHIDLYQMHHIDRSAPWDEVWQAMELLVAQGKVIYVGSSNFAG
                     WHIAQANEAARSRHFLGLVSEQSLYNLGARTVELEVLPACRAYGVAVIPWSPLAGGML
                     GGGRDATEGRRASVNPRWANMAPQLERWEAWCSARGEEPAAVALAWLLHQREVVCPII
                     GPRTAAQLDGASMQALDIVLDEQACKELDEIFPGPGGRAPEAYAW"
     gene            1109..>2006
                     /locus_tag="VIX62_06280"
     CDS             1109..>2006
                     /locus_tag="VIX62_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5859875.1"
                     /translation="MGASRFDRGLVLGAFLPPDVGHHALIHHVQARCAQVVIAVTDRP
                     GQLPETHDRASWMQAVHLDLDVVVLPDACEWHESAPCVDECASAWADQVALLPAPFDL
                     IVTAHSFGPLLADRLGATYLDPSTLTSADLGPVPIPGSTTGDDDAMCRDLAGNWDRLH
                     PVVRAGLYRRVALIGSESTGTTTLARDLAEHLQAPLTYEAGRIMSWSLAARAGGIEKV
                     EWTEHDFFRILEEQRRVEADATARAAELPIGPLGPWLVCDTDALATVVWWERYLVGPP
                     DPSMRFSDARLADAYVITSPHDV"
BASE COUNT          295 a          690 c          693 g          328 t
ORIGIN      
        1 tcctaccccg cccaccccga acaggttccc gttcgccagt ggcgacggcg tagcttcgac
       61 ctcatggaat accgccggct cggccgcacc gcccttcgcg tcagtccact gtgcctcggc
      121 acgatgaact tcggcctgca gaccgaggaa gccgactcgc acgccatcat ggatcgcgcc
      181 ctcgagctcg ggatcaactt cttcgacact gcgaaccgct atgggggcag tcttggcgct
      241 ggcggcacag aggagatcat cgggcgctgg ttcgcgctcg gtggtggccg gcgggagaag
      301 gtggtgctcg ccacgaaggc gttcggagcg atgtccgaat ggccgaacga cggaggtctg
      361 tcggcccggc acatccgtcg cgcctgtgaa gagagtctcc ggagactgca gaccgatcac
      421 atcgacctct accagatgca tcacatcgac cgctcggcgc cctgggacga ggtgtggcag
      481 gcgatggaac tgctcgttgc gcagggcaag gtgatctacg tcggcagctc gaacttcgcc
      541 ggatggcaca tcgcgcaggc gaacgaagca gccaggtcgc gccacttcct cgggctggtg
      601 agcgagcaga gcctctacaa cctcggcgcc cgcacggtgg agctcgaagt gctcccggcc
      661 tgccgcgcct atggcgtcgc cgtcatcccg tggagcccgc tcgccggcgg catgctcggg
      721 ggcgggcgcg atgcgacgga ggggcggcgg gcgagcgtca atccccgttg ggcgaacatg
      781 gcaccgcaac tcgagcgctg ggaggcgtgg tgctccgcac gaggtgagga gccggccgcg
      841 gtggcgctgg catggctgct gcaccagcgc gaggtcgtct gcccgatcat cgggccgcga
      901 acggccgcac agctcgacgg tgcgtcgatg caggcgctcg acatcgtcct cgacgagcag
      961 gcgtgcaagg agctcgacga gatctttccc ggacccggtg gtcgggctcc cgaggcgtac
     1021 gcctggtagt cgtcggccgc gccgatcgat ccgagcgctc gcggcggcga tggcggatcc
     1081 gtcggcctcg ccagtaagac tcgcgggcgt gggcgcctct cgcttcgatc gtggactcgt
     1141 gctcggggcg ttcctgccgc ccgacgtcgg tcaccatgcg ctcatccatc acgtgcaggc
     1201 gaggtgcgcg caggtcgtga tcgccgtgac ggatcgtccg ggtcagctcc ccgagaccca
     1261 cgaccgggca tcgtggatgc aggccgtcca cctcgacctc gacgtcgtcg tgctccccga
     1321 tgcgtgcgag tggcacgaat cagcgccgtg tgtcgacgaa tgtgcttcag catgggccga
     1381 tcaggtggca ctgcttccgg cacccttcga cctgatcgtc accgcccatt cgttcggtcc
     1441 tctcctcgcc gaccggctcg gtgccaccta cctcgatcca tcgacgctca cctcggccga
     1501 tctgggcccc gtaccgatcc ccggctcgac cacgggcgac gatgacgcca tgtgccgcga
     1561 cctcgccggt aactgggatc gcctgcaccc ggtggtgcga gccggcctgt atcgccgggt
     1621 ggccttgatc ggctcggagt cgacggggac gacgacgctg gcgcgcgatc ttgccgagca
     1681 cctgcaggcg ccgttgacgt acgaggccgg caggatcatg tcgtggtcgc tcgccgcccg
     1741 tgccggtggc atcgagaagg tcgagtggac cgagcacgac ttcttccgga tcctggagga
     1801 gcagcgtcgg gtcgaggccg atgccaccgc ccgtgccgcc gagctcccga tcggcccgct
     1861 cggaccgtgg ctggtgtgcg acacggatgc gctggccacc gtggtgtggt gggagcggta
     1921 cctcgtcggg ccgcccgatc cgagcatgcg gttctccgac gcccgcctcg ccgatgccta
     1981 cgtgatcacg agcccgcacg acgtgt
//
LOCUS       DATDBZ010000258         8506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1925008, whole genome shotgun sequence.
ACCESSION   DATDBZ010000258 DATDBZ010000000
VERSION     DATDBZ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925008"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VIX62_06285"
     CDS             <1..407
                     /locus_tag="VIX62_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEY5859876.1"
                     /translation="FPIEVFDRTDGKPPRWPTFGIKLSAKPSAPELQRFEVKGALGLA
                     RAMVGTMTNFHDQMHIDDPSVLARTMFVDTQHVKATDFDLDEPSQDMLFTNGERGAGK
                     FLSGWDFERYVAEFRSDTTIDLTAEHPRVDAG"
     gene            523..1116
                     /locus_tag="VIX62_06290"
     CDS             523..1116
                     /locus_tag="VIX62_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859877.1"
                     /translation="MISLPIGDASNGLCGGMVFAARDFFETERPIPDEDAPPGSGPLF
                     DYLCDRLFESFDLPWGPTRYLTLMNPVLPDGETVWSRLGIGPHGRAWQTIHDAWPQVR
                     ADIDAGHPSPLGLVTVKSSDPWQLKHDHQVLAYGYELDEGILALSVYDPNLPGRDDVR
                     LSLSIADPWHLTVMTMLPSARPVYAFFRVAYEAATPP"
     gene            1166..2953
                     /locus_tag="VIX62_06295"
     CDS             1166..2953
                     /locus_tag="VIX62_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY5859878.1"
                     /translation="MASQIEDYGLIGDLQTAALLGRDGSIDWCCFPRFDSGACFSALL
                     GGPDDGRWLLAPSEPFQVISRRYRHDTLVLESVLETASGSVRMIDFMPPRGDTPDIVR
                     IVEGLTGSVPMTLELTIRFDYGHIVPWVRQVDDALVAIAGPDALCLWSPVPTRGQGMK
                     TMASFTVDAGQRIPFVLTWFPSHQPTPDPIDAEVALDDAEGFWLDWSGTCVNDGDYHE
                     EIRESLLVLKAMTFQPTGGIVAAPTTSLPEEPGGERNWDYRYCWLRDATLTLLAMLRA
                     GAKDEAVNWRGWLLRAMAGDPADLQIMYGLSGERRLDERILEWLPGYEGSTPVRVGNA
                     ASEQLQLDVYGDVIDALYQTRRQGAPADDNVWSLTRKLLEWLEEGWKLPDHGIWEVRG
                     PARHFTHSKVMAWVAFDRAVRATEEWGREGPVERWRAIRDEIHAQVIERSWSEHHQAF
                     AQSYGSDELDASVLLMAPVGFMPAADPRFVSTVSAVERELVSGGLVMRYRARADGAID
                     GITSDEAAFLPCSFWLADALALIGEFDRARELFERLLDLRNELGLLSEEYDPVANRQL
                     GNYPQAFTHLALINTAFVLREGRPLRGRG"
     gene            complement(2977..3246)
                     /locus_tag="VIX62_06300"
     CDS             complement(2977..3246)
                     /locus_tag="VIX62_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859879.1"
                     /translation="MGEAADRKVKEIEETRRQVEADLSELEARIPSPLRSVKTLVGTA
                     VTSSAVAGLTGMWMKKRRAKRRADDRTTEVVVRVVRDDVEVEGTG"
     gene            complement(3219..4601)
                     /locus_tag="VIX62_06305"
     CDS             complement(3219..4601)
                     /locus_tag="VIX62_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY5859880.1"
                     /translation="MDIAAPIRKLDGFQRTHPWMGFPLAVVKRFGDSGAGSLAAALAY
                     YGFFSLFPLLMVFTSLAGIILRDRPDLQDQLLDSALAQFPVIGTQIRSNVGQIDGSGL
                     TLAIGIVLALWAGLGAVRAAQVAMDTIWDVPRKHRRGTPASIAMALAMLGVLGVFVLG
                     GAVLAGLATTASGTAGSLVALAGSAVLNVVLFAVAYRVLTAADLTWRQVLPGACLAGL
                     GWTVLLSLGGWIVADRVSSSSDVYGTFALVIGLLAWIYLGAQLTLYGAEVNAVQQGRL
                     WPRSLQGELTEADRRALRRSARQEERKEEEIVDVTFGDDGDPKASDPTVGSGATRTTT
                     AERRSVAAVARSVIDGVTQLFRHEVELAKIEATEAIAVRAQAIGLLAAGGVLALYALG
                     YLAASGAAALDIVLPAWASRLIVAGVFIIGGAILFLAGRAAMRSAGGVEQTQATVKED
                     VQWAKQQIAR"
     gene            4680..4862
                     /locus_tag="VIX62_06310"
     CDS             4680..4862
                     /locus_tag="VIX62_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859881.1"
                     /translation="MAEDPRSELDRVIQDLQRMEQAQGSATAAGGMLHAARTILLEVA
                     RITEFGGAVTDDEPAG"
     gene            complement(4859..5857)
                     /locus_tag="VIX62_06315"
     CDS             complement(4859..5857)
                     /locus_tag="VIX62_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY5859882.1"
                     /translation="MTKGILERLAEGPVLGDGGYLLELEKRGYVQAGPFTPEVVIEHP
                     DALAQLHREFLMAGAEVLQTMTFYASDDKLATVGLADRVDDINRTAVRIAREVATEGD
                     ALVAGNLSLTWAYDPADAASPDHVRALLDRQLQDQLDAGPPDFWIGETFSFLGEALLF
                     VERAKQTGLPVMVTMSFETLPAKAYEGDTAGEAAKKLADAGADIVGVNCLNSPVQQLP
                     IAVEMVKAVRGAVPVAAQPVAYATTEERPDFTSFDEFPTELTAMTLPRGDLAGFAADA
                     REAGVRYIGSCCGSVAEHVREMAKVLGKRSSHEREWRSSTGKAMSAYEYYEGERTS"
     gene            6003..7274
                     /locus_tag="VIX62_06320"
     CDS             6003..7274
                     /locus_tag="VIX62_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HEY5859883.1"
                     /translation="MNAKRIGCIVAVTLCCSQASALAAPGDLDPSFGGDGVVHTNLSS
                     DRRGRDTAFGLAVQADGSIVVVGRAGPRFGVVRYDDDGTLDVGFGQGGKVRTDVGPGP
                     DTASDVALQSTGRIVVAGTSTGPGGGFAVVRYRANGTLDPSFGDHGAVLTSFPGADEG
                     AMDVAIDAEDRIVVVGHAGGAFVTVRYETDGRLDDSFSADGVARTNLTRRKDVAAAVA
                     IQADGAIVVVGTARFEPGGAGQRWAEVRYEESGQLDDTFHLDGVRIEELSSYGNVAFD
                     VAIGNDGQILVVGTIGVGGFDTDTRSGVIQLDPSGQLDPSFSTSGIRSFNVTNRPDTA
                     TSVVIQDDDDIVVAGVSAAGGPFLPRAWVVRYQPNGDLDHTFSDGRVLTDPAAARIAN
                     DVAIQPDGDVVIVGRAGTRSATFATLRLLGA"
     gene            complement(7318..>8506)
                     /locus_tag="VIX62_06325"
     CDS             complement(7318..>8506)
                     /locus_tag="VIX62_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859884.1"
                     /translation="EDPCTDADLDRHRGMLAGERSLIGGASEEAVETLTQVLARTREA
                     GSTADTVAAAELLSRALVEVGRFDEANDVARSAIQGVPADDHDTMARAIGAFGTVQIH
                     RYALQSAVEALRRAVAEAELGEDRVRLVHLRSDLATACAMAGDVAQALEAMLAAREVA
                     VEIGYRRHLALSVSNEAELRLLLGDDRAVTTLSLRGLHAATSLGDIGLACDNLLRLGA
                     DPDLEPADRRAILQASIPIEVALHRPHTLIEFRTVAVEVDALLGDSMYDAGDVLAEAR
                     SLGRPDLELRVLSALGSNADRADLDDLSARVDEAGDRFLLDVELHRITGNRSQKALLR
                     TRGLELYRKAPYAAYRDVLAELGATDPPTDVVVPPMVTQPDDPVYSRTSVLEVIGSLR
                     KPG"
BASE COUNT         1299 a         2967 c         2922 g         1318 t
ORIGIN      
        1 acttccccat cgaggtgttc gaccggacgg acggcaagcc gccccggtgg cccaccttcg
       61 ggatcaagct ctccgccaag ccgtcggcgc ccgagctgca gcgcttcgag gtgaaggggg
      121 cgctgggtct ggcgcgagcc atggtgggga cgatgacgaa cttccacgac cagatgcaca
      181 tcgacgatcc gtcagtcctg gcgcggacga tgttcgtcga cacccaacat gtgaaggcca
      241 ccgacttcga tctcgacgaa ccttcacagg acatgctgtt cacgaacggg gagcggggcg
      301 ccgggaagtt cctgtcgggg tgggacttcg agcgctacgt cgcggagttc cgcagcgaca
      361 ccacgatcga cctcacggcc gaacaccccc gggtcgacgc gggctgagcg ggagggcagc
      421 atcgtcggcc gctcggtccc cgggttcctg cccagtacct cggggttccg gttcgccaac
      481 gacttcccgc acgtcccggt gcgcacgatc gggatcccgg gtgtgatcag cctcccgatc
      541 ggcgacgcgt cgaacgggct gtgcggcggc atggtgttcg cggcccgcga cttcttcgag
      601 acggagcgtc ccatccccga cgaggacgcc ccgccgggtt cggggccgct gttcgactac
      661 ctgtgcgacc gcctgttcga aagcttcgac ctgccctggg ggcccacccg ctacctgacg
      721 ttgatgaacc ctgtgctgcc cgacggcgag acggtgtgga gccggttggg catcggtccg
      781 cacgggcgcg cgtggcagac gatccatgac gcgtggccgc aggttcgcgc cgacatcgac
      841 gccggtcatc cgtcgccgct gggcctcgtc accgtgaagt catcggaccc ctggcagctc
      901 aagcacgatc atcaggtgct cgcctacggc tacgaactgg acgaagggat cctcgccctc
      961 agcgtctacg acccgaactt gccgggccgc gacgacgtcc gactgtcgct gagcatcgcc
     1021 gatccctggc atctcacggt gatgacgatg ctcccgtcgg cgagaccggt ctacgccttc
     1081 ttccgggtcg cgtacgaggc cgcgacgcct ccttagcggg cgttcgggga cagccccttc
     1141 cgacatgcct ggctagtctg gcccgatggc atcccagatc gaggactacg gcctgatcgg
     1201 cgacctccag acggctgctc tgctgggtcg cgacggctcg atcgactggt gctgcttccc
     1261 gcggttcgat tccggcgcgt gcttctcggc gctgctcgga ggacccgacg acggtcgctg
     1321 gctcctggct ccgtccgaac ccttccaggt gatcagccgg cgctaccgcc acgacacgct
     1381 ggtgttggaa tcggtgctgg agacggcctc ggggtccgtg cgcatgatcg acttcatgcc
     1441 gccccgcggc gacacgcccg acatcgtgcg gatcgtcgaa gggctgaccg gctcggtgcc
     1501 catgacgctc gaactgacga tccgcttcga ctacgggcac atcgtgcctt gggtccggca
     1561 ggtcgatgac gcgctcgtcg cgatcgccgg gccggacgcg ttgtgcctgt ggtctccggt
     1621 gccgacgcgg gggcaaggca tgaagacgat ggcctcgttc acggtggacg ccgggcagcg
     1681 tatccccttc gtcctgacat ggttcccgtc gcatcagccc acgcccgatc cgatcgatgc
     1741 cgaggtggcc ctcgacgacg ccgaggggtt ctggctcgac tggtcgggca cctgcgtgaa
     1801 cgacggcgac taccacgagg agatcaggga gtccctgctg gtgttgaagg cgatgacgtt
     1861 ccaaccgacg ggcggcatcg tggcggcgcc gaccacctcg ttgcctgagg agcccggcgg
     1921 cgagcggaac tgggactacc gctactgctg gctgcgcgat gccacgttga ccctgctcgc
     1981 gatgctccgc gccggagcca aggacgaggc cgtcaactgg cgtggatggc tgctgcgcgc
     2041 catggcgggg gatccggcag acctgcagat catgtatggc ctctcgggag agcgacgcct
     2101 cgacgaacgc atcctggaat ggttgcccgg ctacgagggg tcgacgccgg tccgggtggg
     2161 gaacgcggcg tccgaacagc tgcagctcga cgtctacggc gatgtgatcg acgcgctgta
     2221 ccagacgagg cggcagggag cgccggccga cgacaacgtg tggtcgctga cccgcaagct
     2281 gctggaatgg ctcgaggagg ggtggaagct gcccgaccac ggcatctggg aggtgcgcgg
     2341 tcctgcccgt cacttcaccc actcgaaggt catggcgtgg gtcgccttcg atcgcgcggt
     2401 ccgagccacc gaggaatggg gacgtgaagg cccggtcgag cgatggcggg cgatccgcga
     2461 cgagatccac gcgcaggtca tcgagcggtc ctggagcgaa caccatcagg cgttcgcgca
     2521 gtcgtacggt tccgatgaac tggacgccag cgtgctgctg atggcgccgg tgggcttcat
     2581 gcccgccgcc gacccacggt tcgtctcgac ggtgtcggcg gtcgagcgcg agctggtctc
     2641 aggcgggctg gtgatgcgat accgcgcccg tgccgacggg gcgatcgacg gcatcacctc
     2701 cgacgaggca gcgttcctgc cgtgctcgtt ctggctcgcc gacgcgctcg ccctgatcgg
     2761 ggagttcgac cgggcccgcg aactgttcga gcgcctgctg gacctgcgca acgaactggg
     2821 gctgctctcc gaggagtacg accccgtcgc gaaccggcag ctggggaact acccccaggc
     2881 gttcacgcac ctcgcgctga tcaacacggc gttcgtcctg cgcgaaggcc ggccgctccg
     2941 cggccgcgga tagacgcgcg tctccgtcgc tgagggtcag cctgttcctt ccacctccac
     3001 gtcgtcacgg acgacccgca cgacgacctc ggtggtgcgg tcgtctgccc gccgcttcgc
     3061 gcgtcgcttc ttcatccaca tcccggtgag gccggcgacg gccgagctgg tgaccgcggt
     3121 gccgacgagg gtcttcaccg atcggagggg cgaggggatc cgcgcctcca gttcgctgag
     3181 gtcggcctcg acctgccgtc gggtctcctc gatctccttc accttgcgat ctgctgcttc
     3241 gcccattgga cgtcctcctt caccgtcgcc tgtgtctgct cgaccccgcc cgccgaccgc
     3301 atcgcggcgc gcccggccag gaacaggatc gccccgccga tgatgaacac gccggccacg
     3361 atcaaccggg acgcccacgc aggcagcacg atgtccaacg ccgccgcgcc cgaagccgcc
     3421 aggtacccga gcgcgtacag cgccaacacg ccacccgccg cgagcagccc gatcgcctgt
     3481 gcgcgaaccg cgatggcctc ggtcgcctcg atcttcgcca gttccacctc gtgacggaac
     3541 aactgggtga ccccgtcgat cacgcttcgc gcgaccgccg ccaccgaccg tcgttccgct
     3601 gtcgtcgtcc gcgtggctcc cgatcccacc gtaggatccg aggccttcgg atcgccgtcg
     3661 tctccgaacg tcacgtccac gatctcctcc tccttgcgtt cttcctgcct cgccgatcgg
     3721 cgaagggctc gccggtcggc ctcggtcagc tccccttgca acgaacgcgg ccagagccgg
     3781 ccctgttgta ccgcgttcac ctccgcgccg tacagcgtca gttgggcccc gaggtagatc
     3841 cacgccagca ggccgatcac cagcgcgaag gtgccgtaca catcggagga cgaggacacg
     3901 cgatcggcca cgatccaccc tccgaggctc agcaggaccg tccatccgag tcccgcgaga
     3961 caggcaccgg gcagcacctg acgccaggtg aggtcggcgg ccgtcaggac gcgataagcc
     4021 acggcgaaga gcaccacgtt gaggaccgcc gagcccgcca gggccacgag ggatcccgcg
     4081 gtgccgctgg cggtggtcgc cagcccggcg agcaccgcgc caccgaggac gaagacgccc
     4141 agcaccccca gcatggccag cgccatcgcg atcgaggccg gcgtcccgcg ccgatgcttc
     4201 cgggggacgt cccagatggt gtccatcgcg acctgcgccg cgcgcacggc gccgagcccc
     4261 gcccacagcg ccaggacgat cccgatggcc agcgtcaggc ccgagccgtc gatctgtccg
     4321 acgttgctgc ggatctgggt gccgatcacc gggaactggg ccagggccga gtccaggagt
     4381 tggtcctgga ggtcggggcg gtcgcgaagg atgatcccgg ccagcgacgt gaagaccatc
     4441 agcagcggga acaacgagaa gaacccgtag taggcgaggg cggccgcgag cgaaccggcc
     4501 ccgctgtcac cgaagcgctt gacgacggcc aaggggaagc ccatccatgg gtgggttcgc
     4561 tggaacccgt cgagcttcct gatcggtgcg gcgatgtcca cggtggagcc attccctcag
     4621 ggaggcaaag gaaacgggag gccgaacgca tcgggcatgc ctgtggcacg cttcacccca
     4681 tggctgaaga ccctcgcagc gagctcgacc gcgtgatcca ggacctccaa cgcatggagc
     4741 aggcacaggg ctccgcaacc gcggcgggcg ggatgctgca cgccgcgcgg acgatcctgt
     4801 tggaggtggc gcgcatcacc gagttcggcg gcgccgtcac cgacgacgag ccggcgggct
     4861 agctggtgcg ctcgccctcg tagtactcgt aggccgacat cgccttgccg gtgctcgagc
     4921 gccactcgcg ttcgtgcgag gagcgtttgc ccagcacctt ggccatctcg cgcacgtgct
     4981 cggccaccga tccgcagcac gagccgatgt agcgcacgcc cgcctctcgg gcgtcggcgg
     5041 cgaacccggc gaggtcgccg cgggggagcg tcatcgcggt gagctcggtg gggaactcgt
     5101 cgaacgacgt gaagtccggt cgttcctcgg tggtggcgta ggcgaccggc tgggccgcca
     5161 ccggcaccgc accacggacg gctttcacca tctcgacggc gatcggcagc tgctggacgg
     5221 gggagttgag gcagttcacg cccacgatgt cggcgccggc atcggcgagc ttcttcgcgg
     5281 cttcgccggc ggtgtcgccc tcgtaggcct tcgccggcag ggtctcgaac gacatcgtca
     5341 ccatcaccgg cagaccggtc tgcttcgcgc gttcgacgaa caggagcgcc tcgcccagga
     5401 acgagaacgt ctcgccgatc cagaaatcgg gcggtccggc atcgagctga tcctggagct
     5461 ggcgatcgag cagcgcgcgg acgtgatcgg gactggcggc atccgcaggg tcgtacgccc
     5521 acgtgaggga gaggttgccg gccacgaggg cgtcgccctc ggtggcgacc tcgcgagcga
     5581 tgcgcaccgc cgtgcggttg atgtcgtcga cccggtcggc cagtcccacg gtcgcgagct
     5641 tgtcgtcgct ggcgtagaag gtcatggtct gcagcacctc ggcgcccgcc atcaggaact
     5701 cgcggtgcag ctgcgccaac gcgtccggat gctcgatcac cacctccggc gtgaacggac
     5761 ccgcttgcac gtacccgcgc ttctccagct cgagcaggta gccgccgtcg cccagcaccg
     5821 gaccttcggc cagccgttcc aggatcccct tcgtcacgtg accgccccct tggactcagc
     5881 accgagtgta cgagtcgtac ctgcagcccc ggcctccggc gaggcaccgc gggtcacacc
     5941 gcgtccactc ccgatcgaag cggcacgcag gtcgcgggcg tatggtcctc gccaggagga
     6001 cgatgaacgc caagcggatc ggctgcatcg tggcggtgac gttgtgctgc tcccaggcca
     6061 gcgccctggc cgctcccggc gacctcgacc cctcgttcgg tggggacggc gtcgtgcaca
     6121 ccaacctctc ctcggatcga cgcggccggg acacggcgtt cggtctcgcg gtgcaagccg
     6181 atggttcgat cgtcgtcgtg ggacgtgcgg gcccgcggtt cggcgtcgtc cgctacgacg
     6241 acgacggaac cttggacgtg gggttcggcc aaggaggcaa ggtccggacc gacgtcggcc
     6301 cgggtccgga caccgcctcc gatgtcgcgc tgcaatcgac cggcaggatc gtggtggccg
     6361 gcacctcgac gggaccgggt ggcggattcg ccgtcgtgcg gtaccgggcg aacggcacgc
     6421 tcgatccctc gttcggcgat cacggggccg tgctgacctc gttcccaggg gccgatgagg
     6481 gggcgatgga cgtcgcgatc gacgccgagg accggatcgt cgtggtgggt catgcgggcg
     6541 gcgccttcgt caccgttcgg tatgagaccg acggccggct cgacgactcg ttctcggccg
     6601 acggcgtcgc ccgcacgaac ctcaccaggc ggaaggatgt cgcggcggcg gtggcgatcc
     6661 aggccgacgg tgcgatcgtg gtggtcggga cggcacgctt cgaaccaggt ggagccggcc
     6721 agcgctgggc ggaggtgcgg tacgaggaga gcgggcaact cgacgacacc ttccatctcg
     6781 acggcgttcg tatcgaggaa ctgtcgagct acggcaacgt cgccttcgac gtggcgatcg
     6841 gcaacgacgg tcagatcctg gtggtgggca cgatcggcgt gggggggttc gacaccgaca
     6901 cccgctcggg ggtgatccaa ctcgatccgt ctgggcagct ggatccgagc ttcagcacct
     6961 cgggcatccg cagcttcaac gtcacgaacc ggccggatac ggcgacctcg gtcgtcatcc
     7021 aggacgacga cgacatcgtc gtggcgggcg tctcggcagc cggcggcccg ttcctgccga
     7081 gagcatgggt ggtgcgctac caaccgaacg gggatctcga tcacacgttc agcgacggca
     7141 gggtgctcac ggatccagcc gcagcgcgga tcgcgaacga cgtcgcgatc cagcccgacg
     7201 gtgatgtcgt catcgtcggc agggccggga cccgctccgc gacattcgcg acccttcgac
     7261 tcttgggcgc ttgaggacta ggtgtagccc cagcgcgtgt ggtcgtgaac gcggccgtca
     7321 tccgggcttg cgcagcgacc cgatgacctc gagcaccgag gtgcgcgagt agaccggatc
     7381 atcgggctgc gtcaccatcg gcggaacgac gacgtcggtg ggtggatcgg tggcacccag
     7441 ctcggccagc acatcgcggt aggcggcgta cggcgccttc cgatagagct cgaggccgcg
     7501 cgtgcggagc aaggccttct gcgatcggtt cccggtgatc cggtgcagct ccacgtcgag
     7561 caggaaccgg tcgccggcct catccacgcg ggcgctcaga tcgtcgaggt cggcacggtc
     7621 ggcgttcgac cccagcgccg acagcacccg tagttcaagg tctgggcgtc caaggctccg
     7681 cgcttcggcg agcacatcgc cggcgtcgta catcgaatct cccaggaggg cgtccacctc
     7741 caccgccacc gtccggaact cgatcagggt gtgcggccga tgcaacgcga cctcgatggg
     7801 gatcgaggcc tgcaggatcg cgcgccggtc cgccggctcc aggtcggggt cggcgcccag
     7861 gcgcagcagg ttgtcgcagg ccagtccgat gtctccgagc gaggtcgccg cgtgcagccc
     7921 gcgcagggac aacgtggtga ccgcgcggtc atcgccgagg aggagccgaa gctcggcctc
     7981 gttgctcacg ctgagcgcga ggtgccggcg gtagccgatc tcgaccgcga cctcgcgcgc
     8041 ggcgagcatc gcctccaacg cctgcgcgac gtcgcccgcc atcgcgcacg ccgtggccag
     8101 gtcgctccgg agatggacga gccgaacccg gtcctcaccc agttccgctt cggccaccgc
     8161 ccgtcgcagg gcctcgacgg cgctctgcag ggcatatcga tggatctgca ccgtgccgaa
     8221 ggcgccgatg gcgcgcgcca tggtgtcgtg atcgtccgcc gggacccctt ggatcgccga
     8281 cctggcgacg tcgtttgcct cgtcgaagcg gccgacctcg acgagcgccc ggctcaacag
     8341 ctcggcggcg gccaccgtgt ccgccgtcga acccgcttcc cgggtgcgag ccagcacctg
     8401 cgtcaaggtc tcgacggcct cctcgctcgc cccgccgatc aacgagcgtt cgcctgccag
     8461 catccctcgg tgtcgatcga ggtcggcatc ggtgcaaggg tcttcg
//
LOCUS       DATDBZ010000259         3625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2934803, whole genome shotgun sequence.
ACCESSION   DATDBZ010000259 DATDBZ010000000
VERSION     DATDBZ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2934803"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..157)
                     /locus_tag="VIX62_06330"
     tRNA            complement(86..157)
                     /locus_tag="VIX62_06330"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Val,seq:cac)
     gene            complement(222..1043)
                     /locus_tag="VIX62_06335"
     CDS             complement(222..1043)
                     /locus_tag="VIX62_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY5859885.1"
                     /translation="MRAWVNGQLLSDPTAPAVQIMDHGFTVGDGVFEAAKVVDGQPFA
                     ITRHLARLARSADRLGLPMPDDGEVRRGIDAVLDGFEEPLGRLRITYTGGPAPLGSGR
                     GEDPPTLVVALAPLGRHAETTSAVTVPWPRNERGALAGVKSTSYAENVVALAYATERG
                     ATEAVFPNLQGHLCEGTGSNVFYVLDGELRTPTLASGCLAGITRALILEWCDAVEVDE
                     PIEAVTQRASEIFLASTTRDAQPVTRWNDRQLDVGPLTQAVRAEWRAREAAHLDP"
     gene            1167..1239
                     /locus_tag="VIX62_06340"
     tRNA            1167..1239
                     /locus_tag="VIX62_06340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1200..1202,aa:Gly,seq:gcc)
     gene            1268..1338
                     /locus_tag="VIX62_06345"
     tRNA            1268..1338
                     /locus_tag="VIX62_06345"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1300..1302,aa:Cys,seq:gca)
     gene            1358..1432
                     /locus_tag="VIX62_06350"
     tRNA            1358..1432
                     /locus_tag="VIX62_06350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1390..1392,aa:Val,seq:gac)
     gene            complement(1531..2202)
                     /locus_tag="VIX62_06355"
     CDS             complement(1531..2202)
                     /locus_tag="VIX62_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY5859886.1"
                     /translation="MAKFKNPASTSVVIIGLGRFGSAVADSLVRMGQEVLAIDENQTL
                     VDRWADDLTHVAQADTTDDQALRQLGVGDFDRAVVAIGTDIEASVLTVLALAEAGVPD
                     IWAKAITKKHGAILERVGAHHVIYPEFSMGERVAHMLTGQMMDYIEFDDEFSIARTLA
                     PSFTWGKTLAESAPRSKYGVTIVGVKRTGEDFTYARPETSVERHDHLIVSGPPSKVEK
                     FCNIR"
     gene            complement(2195..3607)
                     /locus_tag="VIX62_06360"
     CDS             complement(2195..3607)
                     /locus_tag="VIX62_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEY5859887.1"
                     /translation="MTDGRRVHRYGVMVCLLGQERSKHVRSVSDGFAHPARIVPLAFL
                     AAIVVGTVLLSLPYSTPGPTRPPVLTAAFTTVSAVCVTGLTTVDTATYWSPFGQTVIM
                     ALIQVGGFGIMTLATLLGMLVGGKLRLKSSLIAQAETHTLNIGDVRHVVRRVAITMLA
                     FEVVFAAILTARFRARYDDTWGDAVWHGVFHSISAFNNAGFALYSDNLIGFVADAWIM
                     FPICAAIIAGGIGFPVLFELRRRWRVPHQWTVHTRLTVYGSLILLVAGIGAFLALEWS
                     NEGTLGGLGPWGKVVGGVTGGVVPRTAGFNSIDYANITPETMAVNYVLMFIGGGSGGT
                     AGGIKVTTFFLLAFVIWSEVRGERDTNIAHRQIGSQTLRQALTVVLLALAAIAVGTMM
                     ILLVTDYALDVVLFETISAFATVGLSTGITPNLPPPAEITLMVLMFVGRVGTITVASA
                     LALKSRHRQFHLPEERPVIG"
BASE COUNT          628 a         1145 c         1291 g          561 t
ORIGIN      
        1 cggggtcgtg aggtggggcg ggcacaaagt ggcccgaagg agcgcgctga tcgtggaatg
       61 tgcctctgac cagcgcgttt ggaagtgggc gatactgggt tcgaaccagt gacctcttcg
      121 gtgtgaacga agcgcgctac cactgcgcca atcgcccgcg tggtgcggcg ggaactctaa
      181 cgcatgagcc ccagcagcct cacatcgaga gtggtggcga ctcacgggtc caggtgggcg
      241 gcctcgcggg cccgccactc ggcgcgcacg gcctgggtga gggggccgac atcgagctgg
      301 cggtcgttcc agcgggtgac gggctgcgcg tcgcgggtgg tcgaggcgag gaagatctcg
      361 ctcgcccgct gggtcaccgc ctcgatgggc tcgtcgacct cgacggcgtc gcaccactcc
      421 aggatcagcg cccgggtgat gccggccagg cagcccgacg ccagggtcgg cgtccggagc
      481 tcaccgtcga gcacgtagaa gacgttggag ccggtgccct cgcacagatg accctgcagg
      541 ttcgggaaga ccgcctcggt ggcgccacgt tctgtggcgt aagccagggc gacgacgttc
      601 tcggcgtacg acgtcgactt caccccggcc agcgcgccgc gctcgttgcg gggccacggc
      661 acggtgacgg cgctcgtcgt ctccgcgtgg cggccgagcg gcgcgagggc gacgaccagg
      721 gtcggcgggt cttcgccgcg tccggagccg agcggagccg ggcctccggt gtaggtgatg
      781 cgcaggcgcc ccagcggctc ctcgaagccg tcaaggacgg cgtcgattcc gcgtcgcacc
      841 tcgccgtcgt cgggcatggg cagtccgagt cggtcggccg agcgggcgag ccgcgcgagg
      901 tggcgcgtga tcgcgaacgg ctgtccgtcg accaccttcg cggcctcgaa gaccccgtca
      961 cccacggtga acccgtggtc catgatctgc accgcggggg ccgtcgggtc gctcaggagc
     1021 tgtccgttca cccacgcccg catgcggcca ctctaggctc ctcgatcggg ctgacacgcg
     1081 ggcccggacc tcgattttgg gcggttcgag gcatcggcta aggttctcac gcacctcgcc
     1141 ggggacccct ccggcgtacg gcgcaagcgg acgtagctca gttggtagag cgcaaccttg
     1201 ccaaggttga ggtcgcgagt tcgagcctcg tcgtccgctc ggagaggtcc ccgcaattgg
     1261 cctccacggt gggttggccg agaggcgagg caacggactg caaatccgtg tacacgggtt
     1321 caaatcccgt acccacctcg aaatacctga atgacggggg cgattggcgc agcggtagcg
     1381 cgcttccttg acacggaaga ggtcactggt tcaaacccag tatcgcccac cagcgaagat
     1441 gcaggtcaga ggccggttcc cgtcagggag ccggcctctg gtgcaacgca ggtgcagcaa
     1501 ggcagcgaac gccgagccag gcgggctcgg tcatcggatg ttgcagaact tctcgacctt
     1561 gctcggaggt ccggacacga tgaggtgatc gtgacgctcg accgaggttt cgggccgggc
     1621 gtaggtgaag tcctcaccgg tgcgcttgac tccgacgatc gtcacgccgt acttgctgcg
     1681 tggtgccgac tcggccagcg tcttgcccca cgtgaacgag ggggcgaggg tgcgggcgat
     1741 ggagaactcg tcgtcgaact cgatgtagtc catcatctgg ccggtgagca tgtgagccac
     1801 gcgttcaccc atggagaact ccgggtagat cacgtggtgg gcaccgaccc gttcgaggat
     1861 ggcgccgtgc ttcttggtga tggccttggc ccagatgtca ggcacgcctg cttcggcaag
     1921 cgccagaacg gtcaggacgc tggcttcgat gtcggtgccg atggccacga cggcgcggtc
     1981 gaagtccccg acaccgagtt ggcgcagcgc ttggtcgtcg gtggtgtccg cctgcgcgac
     2041 gtgggtcaag tcatcggccc accgatcgac gagggtctgg ttctcgtcga tggcaaggac
     2101 ctcctggccc atgcgaacga gcgagtcggc cacggcggag ccgaaccggc cgaggccgat
     2161 gatgacgacg gacgtggacg cggggttctt gaacttagcc aatgacgggt cgctcctcgg
     2221 gaaggtggaa ctggcggtgt cgggacttga gggccagcgc tgaggcgacc gtgatggtgc
     2281 cgacgcgccc cacgaacatg aggaccatca gggtgatctc tgctggtggc gggaggttgg
     2341 gggtgatgcc ggtggacaag ccgaccgtgg cgaacgcgga gatggtctcg aacaacacca
     2401 cgtcgagggc gtagtcggtg accagcagaa tcatcatggt cccgacggcg atggcagcca
     2461 gggcgagcag gaccacagtg agcgcttggc gcaacgtctg gctgccgatc tggcggtgcg
     2521 cgatgttggt atcgcgctca ccgcggactt ctgaccagat gacgaacgcc agcaggaaga
     2581 acgtggtgac cttgatgcct ccggcggtgc cacccgagcc gccgccgatg aacatgagga
     2641 cgtagttgac ggccatggtt tcgggggtga tgttggcgta gtcgatgctg ttgaacccgg
     2701 cggtacgggg cacgacgcca ccggtgacac caccgacgac cttcccccac gggcccagtc
     2761 cgccgagggt gccttcgttg gaccactcca gcgcgaggaa ggccccgatc cccgcaacga
     2821 gcaggatcag cgagccgtac acggtcagcc gggtgtgcac cgtccactga tgagggacac
     2881 gccagcggcg gcgcagctcg aacagcacgg ggaagccgat gccgccggcg atgatcgccg
     2941 cacagatggg aaacatgatc caggcgtcgg cgacgaagcc gatgaggttg tcgctgtaga
     3001 gcgcgaagcc ggcgttgttg aacgccgaga tcgagtggaa cacgccgtgc cacacggcat
     3061 cgccccacgt gtcgtcgtag cgagcgcgga agcgtgcggt caggatggcg gcgaacacga
     3121 cctcgaaggc gagcatcgtg atcgccaccc gacgcaccac gtgccgcacg tccccgatgt
     3181 tgagggtatg ggtctcggct tgagcaatga gtgacgactt gaggcgcagc ttcccgccga
     3241 ccagcatgcc caacagggtc gccagcgtca tgatgccgaa cccgccgacc tggatgagcg
     3301 ccatgatcac ggtctgcccg aacggcgacc agtacgtcgc ggtgtccacg gtggtgagtc
     3361 ccgtcacgca caccgccgag accgtggtga aggcagcggt cagcaccggc gggcgagtcg
     3421 gcccgggcgt tgagtacggc agcgagagca ggaccgtgcc gaccacgatc gcggccagga
     3481 acgcgagcgg cacgatgcgc gccggatgcg cgaacccgtc cgagacactg cgcacgtgct
     3541 tactcctctc ctggccgagc agacaaacca tcacaccgta gcgatgcacc cgacgcccgt
     3601 cagtcatccc tacgacacgg cgttc
//
LOCUS       DATDBZ010000260         6821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1087205, whole genome shotgun sequence.
ACCESSION   DATDBZ010000260 DATDBZ010000000
VERSION     DATDBZ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087205"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VIX62_06365"
     CDS             complement(<1..297)
                     /locus_tag="VIX62_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859888.1"
                     /translation="MQTTAHRTRRPSGDPPPLPRDTHATGWLWASLAVVLVGIGLAFI
                     LTNDPDLRELGSEVLEWFADLRTSWLTQVAKVVVLISSFGAVQLLRLGIAGALVA"
     gene            366..1505
                     /locus_tag="VIX62_06370"
     CDS             366..1505
                     /locus_tag="VIX62_06370"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEY5859889.1"
                     /translation="MDEGRTDAVSETPTPGEPPAAEEPKKKRSLTRTIIEGAISAAIV
                     IGIFVFAIPKVADYSAVWKAFQSMTGLELLVLTGAMILNLFTYWLANMAALPGLRLWP
                     AAVLTQTTTSVANTLPGGGAIAVGLTYEILHSWGFTGTNVALYVGVTGIWNIFVKLAL
                     PVLSIAILVITGQSSSAFITAAIVGVVVLAIAVGLLAAIFASERLARSIGNGIGRMTA
                     AILRLFHKEPKSDMGERAVKFRRDTIGLVRLRWIRLTVTTIASQLALFLVLLLALRAM
                     GISEADVSTAKVLAVFSFSRLLSAVPVTPGGVGVIDLGYIGGLAAGLPESEKAAVVAA
                     VLIFRALTYGIQIPIGAFTYLVWRAKKDWRKDVPATPEATSAAAV"
     gene            complement(1502..2212)
                     /locus_tag="VIX62_06375"
     CDS             complement(1502..2212)
                     /locus_tag="VIX62_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5859890.1"
                     /translation="MLTNHRRALRFSLALLAAMVFMLIAVGRHPELEAPKTTLAFVGN
                     FDQSVYHWMDDIRNAFLTGLFRFLNVAGGGKVTIPLRVIVTLLLLFTRRFRAMFAFIL
                     TWAASEALLTWFKIFFHRGRPPMPLVETVGFSFPSGHAVAGAAIAVALVLVAFPPGPR
                     RLKWERGAMAFAFLMGFSRVYLNAHWFSDVVAGVLLGAGIAVGSAAVVTEVANLLEAR
                     KPASVAEPPQDPLDPALD"
     gene            complement(2226..2798)
                     /locus_tag="VIX62_06380"
     CDS             complement(2226..2798)
                     /locus_tag="VIX62_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859891.1"
                     /translation="MRWRSALVVIALVSAGPACTGGGGEDMPVCSDLEAQVLVLEAQS
                     VPSATRLPCVAELPPGWRFGGSLIKDAETTLWLDSDRAGVHALQVTMSASCDVSSAVP
                     VPPAADEVGMTVYQEPKTLEPGFTGWRYLVFEGGCIIHEYAFADGTEPTLVIEADLAI
                     STVARRDIVTYVQDSLDLTLCGAEAPPCVG"
     gene            complement(2795..4483)
                     /locus_tag="VIX62_06385"
     CDS             complement(2795..4483)
                     /locus_tag="VIX62_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5859892.1"
                     /translation="MDTGEQTTREPHERRKRLLGPDARRRPSGEAPPLPREIGRSGKF
                     WLFMVGYFVATLIGVLLFEPMARLYERTDTSFLHLLAEGRNDILTEIMQAIALLSSRW
                     VLRVLRWGTIIALIASRRWRHLFVFLGALITEEVVAYLLTLVIHRPRPLGIEILAPWQ
                     GFSMPSRPMASLAVTLVGMAYSLIPHGRYRDAAKWAIAGVLVGVGIARLYLGVEAASA
                     CVFGGVLGVAIGLTAFRWFTPNDVFPVTYKKGKAAHLDVGGMRGEAIVTAVRDQLGYR
                     VLDIQPVGLAGSGGSTPLRLKVRSEDGGPERQLFAKLYAQNHVRADRWYKIGRTILYG
                     ALEDETPFGTVRRFVEYEDYALRLMYDLELPVPTPYGIVEITPEREYMIVMEFFDDAV
                     EIGEAEVDDGVIEQGLRLVREMWDEGLAHRDIKPANLMVRDGQLKLIDVFFVQVRPSP
                     WRQAVDLANMMMVLALRSDAPRVYEHAVGMFSPDEIAEAFAATRGVASPTQLRSMMKE
                     DGRDLLEEFRALAPNRDEVRIQRWSLRRVGLTVTALLAALFALGLIVTNWAVFA"
     gene            complement(4499..5410)
                     /locus_tag="VIX62_06390"
     CDS             complement(4499..5410)
                     /locus_tag="VIX62_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5859893.1"
                     /translation="MRIRTVVLAMVLALVAASCDRSAGEPSTAVGDDAITVGSFDFPE
                     SEVIAEIYAGALEAEGFRVVREPAIGTREVVLPALQLGLIEVVPEYAGSALTFLGGTA
                     SADPAVTNALLASIVADRGLTALEPAAAENRNGMVVTAETAADLDLRTVSDLAPSADS
                     MVFGGPPECPERDFCLPGLETIYGLHFESFLPLDTGGPLSADAIQRGTVDVGMLFTTD
                     GTLAGDDLVLLRDDRHLQPAESVTPIVRADTLERFGDRARSALDAVSEQLTTEDLREM
                     NASMSAGATPKDVAATWLEDHGLGAAG"
     gene            5466..6401
                     /locus_tag="VIX62_06395"
     CDS             5466..6401
                     /locus_tag="VIX62_06395"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY5859894.1"
                     /translation="MEIVRGLEALPLSDEPAVVTVGFFDGVHLGHQQVFATTVKRARE
                     RGIRSVAVTFDRHPREVLTPGQEPRLLTTVERKASLIEAMGIDVLVVLPFTAAFSRVP
                     AEDFVRDVLVGGIHAVHAAMGANFTFGFKAAGTVETLPAMGEPYGLTAEGVALFELDG
                     RTVSSSSIRDALSAGDLAWPLAALGRRFVLDGEVTSGHGRGKGLGYPTANLRTWPRLM
                     LPGQGIYAGVAEVDGLRYPAAIDVGTNPTFGVEPLHVEAFLLDFEGEELRGKPISIEF
                     WERLRDEIKYDSVEALIEAIAVDVQRTRELVDPSA"
     gene            complement(6403..>6821)
                     /locus_tag="VIX62_06400"
     CDS             complement(6403..>6821)
                     /locus_tag="VIX62_06400"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEY5859895.1"
                     /translation="KGSVKASPGLGPALIGASGVAMVVAGLFRRDRMSNHPEPGQAPG
                     QSGINDVHDAASVVGALTSSVGLFALGARLRHEPGLEDLARPTMVAAAAGSGLLAWFA
                     RGVTRPGNGLVQRAGVTIPLLLMARLAIRLLRTDDG"
BASE COUNT         1103 a         2340 c         2387 g          991 t
ORIGIN      
        1 cgcgaccagc gccccggcga tcccgagccg caacagctgc acggcgccga acgaggagat
       61 caggaccacg accttggcga cctgcgtgag ccaggacgtg cgcagatccg cgaaccactc
      121 caaaacctcc gacccgagct cgcgcagatc cgggtcgttc gtcaggatga aggcgagccc
      181 gatcccgacg agcacgacgg ccagcgacgc ccacagccag cccgtggcat gggtgtcgcg
      241 cggcagcggt ggtgggtcgc cagacggccg gcgggttcgg tgggcggtgg tctgcatgcg
      301 gggcagccta tccggtgcac agggtccgag cgaccctctg gagtcggcgg gtatatcctc
      361 cgcgcatgga cgagggccgc accgatgccg tgagcgagac cccgacgccg ggggagccgc
      421 ccgccgcgga ggaacccaag aagaagcgct ccctcacccg cacgatcatc gagggtgcga
      481 tctcggcggc gatcgtgatc ggcatcttcg tgttcgcgat ccccaaggtg gccgactaca
      541 gcgccgtatg gaaggcgttc cagtccatga ccggcctgga gctcctcgtg ctgaccggcg
      601 ccatgatcct gaacctgttc acttactggc tggcgaacat ggcggcgctg cccgggttgc
      661 gcctgtggcc cgccgccgtg ctgacgcaga cgaccacgtc cgtggcgaac acccttcccg
      721 gcggcggcgc catcgcggtg ggcctgacct acgagatcct gcattcctgg gggttcacgg
      781 ggacgaacgt cgcgctgtat gtgggcgtca ccgggatctg gaacatcttc gtgaagctgg
      841 cgttgccggt gctctcgatc gccatcctgg tgatcaccgg gcagagcagt tcggcgttca
      901 tcaccgcggc gatcgtcggc gtcgtggtcc tggcgatcgc cgtggggctg ctcgccgcca
      961 tcttcgcgag cgagcggctg gcccgctcga tcggcaacgg catcgggcgg atgacggcgg
     1021 cgatcctgcg gctgttccac aaggagccga agtccgacat gggcgagcgc gcggtcaagt
     1081 tccggcgcga cacgatcggg ctggtgcggc tgcggtggat ccggctgacg gtcaccacga
     1141 tcgcgagtca gctcgcgctg ttcctcgtcc tgctgctggc gcttcgggcc atgggcatct
     1201 ccgaggccga cgtcagcacg gcgaaggtgc tggccgtgtt ctccttcagc cggctgctct
     1261 cggccgtgcc cgtcacgccg ggtggcgtgg gcgtgatcga cctgggctac atcggcgggc
     1321 tcgccgccgg cctgcctgag agcgagaagg cagcggtggt ggcggccgtg ctgatcttcc
     1381 gtgcgctgac ctacggcatc cagatcccga tcggcgcctt cacctacctc gtctggcgtg
     1441 ccaagaagga ctggcgcaag gacgtgccgg cgacaccgga agcgacgagc gcggccgcgg
     1501 tctagtccag cgccgggtcg agcggatcct gcggcggttc ggccacgctg gcgggcttcc
     1561 gggcttctag caggttggcg acctcggtca ccaccgccgc cgatccgacc gcgataccgg
     1621 caccgagcag cacacccgcc accacgtcgg agaaccagtg cgcgttgagg tagacccgcg
     1681 agaaccccat caggaacgcg aaggccatgg cgccccgttc ccacttcagg cggcgcgggc
     1741 caggcgggaa ggcgaccaag accaaggcca cagcgatggc ggcgccggcg acggcatggc
     1801 ccgacgggaa cgagaagccc acggtttcca cgagcggcat gggtggacgc ccccgatgga
     1861 agaagatctt gaaccacgtg agcagggctt cggacgccgc ccaggtcagg atgaacgcaa
     1921 acatggcgcg gaaccggcgc gtgaacagca acagcagggt cacgatcacg cgcagcggga
     1981 tcgtgacctt ccccccgccc gcgacgttga ggaaccggaa cagcccggtg aggaacgcgt
     2041 tccggatatc gtccatccag tggtagacgc tctggtcgaa gttcccgacg aaggccagcg
     2101 tggtcttcgg ggcttcgagc tccggatggc gtcccaccgc gatcagcatg aacaccatcg
     2161 ccgccaagag tgccagcgag aagcgcagcg cgcggcgatg gttggtcagc atgggtcggg
     2221 gcagcctatc ccacgcacgg cggcgcctcg gccccgcaca gggtgaggtc caaggagtcc
     2281 tggacgtagg tgacgatgtc acggcgagcg accgtcgaga tcgcaaggtc ggcctcgatc
     2341 acgagggtgg gctcggtccc gtcggcgaac gcgtactcgt ggatgatgca gcctccctcg
     2401 aataccaggt agcgccagcc ggtgaagccg ggttcgaggg tcttgggctc ctggtagacg
     2461 gtcatgccca cctcgtcggc cgcgggaggc accggcacgg ccgacgagac gtcgcacgag
     2521 gcgctcatcg tgacctggag cgcatggacg ccggcgcggt cgctgtccag ccacaaggtg
     2581 gtctcggcgt ccttgatcag ggagccgccg aaccgccacc ccggcgggag ctcggcgaca
     2641 cacggcagcc gtgtcgccga cggcaccgac tgcgcctcga gcacgagcac ctgggcttcg
     2701 aggtccgagc acaccggcat gtcctcgccg ccgccaccgg tgcaggctgg gcccgccgag
     2761 acgagtgcga tgaccacgag cgccgatcgc cacctcatgc gaacaccgcc cagttggtca
     2821 cgatcagccc cagcgcgaac aaggccgcca ggagcgccgt gacggtcagg cccacccgcc
     2881 ggaggctcca ccgctggatg cgtacctcgt cgcggttcgg agcgagcgcc cggaactcct
     2941 cgagcaggtc gcggccgtcc tccttcatca tggagcgcag ctgcgtgggg ctcgcgacgc
     3001 ccctggtcgc cgcgaacgcc tcggcgatct cgtcgggcga gaacatcccg accgcgtgtt
     3061 cgtacacgcg cggcgcatcc gagcgcagag cgagcaccat catcatgttc gccaggtcga
     3121 cggcttggcg ccacggtgac ggtcgaacct ggacgaagaa gacgtcgatc agcttcaact
     3181 ggccgtcgcg caccatgagg ttggccggct tgatgtcccg gtgagcgagg ccctcgtccc
     3241 acatctcgcg cacgagccgc agcccttgtt cgatcacgcc gtcgtcgacc tcggcctcgc
     3301 cgatctccac cgcgtcgtcg aagaactcca tcacgatcat gtactcgcgc tccggcgtga
     3361 tctcgacgat gccgtagggc gtgggcaccg gcagctcgag gtcgtacatc aaccgcagcg
     3421 cgtagtcctc gtactccacg aagcgccgca ccgtgccgaa cggggtctcg tcctcgaggg
     3481 cgccgtagag gatcgtgcgt ccgatcttgt accaacggtc ggcgcgaacg tggttctgcg
     3541 catagagctt ggcgaacagt tgccgctcgg ggccgccgtc ctcggaccgg accttgaggc
     3601 gcagtggggt cgagcctccc gaacccgcca ggcccaccgg ttggatgtcg agcacccggt
     3661 agccgagctg gtccctcacg gcggtcacga tggcttcgcc gcgcatcccg ccgacgtcga
     3721 ggtgggcggc cttgcccttc ttgtaggtaa ccgggaacac gtcgttgggg gtgaaccacc
     3781 ggaaggccgt cagaccgatc gccacgccga ggaccccgcc gaagacgcac gcgctggccg
     3841 cttccacccc gaggtagagg cgggcgatcc cgaccccgac caggacgccg gcgatcgccc
     3901 atttcgcggc gtcgcgatac cgcccgtgcg ggatcagcga gtaggccatg cccaccaacg
     3961 tgacggcgag gctggccatg ggacgcgacg gcatcgagaa cccttgccac ggggcgagga
     4021 tctcgatccc gagcggcctg ggccggtgga tcaccaaggt caacaggtag gccacgacct
     4081 cctcggtgat cagcgccccc aggaagacga acagatggcg ccaccggcgg gaggcgatga
     4141 gggcgatgat cgtgccccac cgcaggaccc ggagcaccca gcgagaggac aacagcgcga
     4201 tcgcctgcat gatctcggtg aggatgtcgt tgcgcccctc ggcgagcagg tgcaggaacg
     4261 aggtgtcggt gcgctcgtag agccgtgcca tgggttcgaa gagcaggacg ccgatcaggg
     4321 tcgcgacgaa gtagcccacc atgaacagcc agaacttgcc gctgcgaccg atctcgcgcg
     4381 ggaggggagg agcttccccc gagggacgcc gccgagcgtc gggacccagg agccgcttac
     4441 gccgctcgtg gggttccctg gtggtctgtt cgccggtatc cacgtcctcg cgccgatgct
     4501 accccgcggc cccgagcccg tggtcctcga gccaggtggc cgcaacgtcc ttgggcgtgg
     4561 ctcccgccga catggacgcg ttcatctcgc gcaggtcctc cgtcgtgagc tgttcggaga
     4621 cggcgtcgag ggccgaacgc gcgcggtcgc cgaaccgctc caaggtgtcc gcgcgcacga
     4681 tcggtgtgac cgactccgcc ggctgcagat gacgatcgtc ccgcaggagc accagatcgt
     4741 cgcccgccaa tgtcccgtcg gtggtgaaga gcatgcccac gtccaccgtg ccacgctgga
     4801 tcgcgtcggc cgagagcggt ccgccggtgt ccaacggcag gaacgactcg aagtgcaggc
     4861 cgtagatcgt ctccagtccg ggcaaacaga agtcgcgttc cgggcattcg ggcggtccgc
     4921 cgaagaccat cgagtcagcc gacggcgcaa ggtcgctgac ggtccgcagg tcgagatcgg
     4981 cggccgtctc ggcggtcacc accatgccgt tgcggttctc ggcggcagca ggttcgagtg
     5041 ctgtgagacc ccggtcggca acgatggatg ccaagagagc gttcgtcact gccggatcgg
     5101 cgctcgcggt accccccagg aaggtgagcg cgctgccggc gtactcgggc acgacctcga
     5161 tgaggcccag ctgaagggcg ggcagcacca cctcgcgcgt gccgatcgcg ggttccctga
     5221 ccacccggaa cccctcggcc tccagcgcgc cggcatagat ctcggcgatc acctcgctct
     5281 cggggaaatc gaacgagccc acggtgatcg cgtcgtcacc gaccgccgtc gagggctcgc
     5341 cagcgctccg gtcgcaggat gcggccacca gggcaaggac catcgcgagc accacggttc
     5401 ggatccgcac cggcgcaccg taccgcgccg aggtggcgcc ggaggcgttc ggtacgctct
     5461 cgcgcatgga gatcgtgcga ggcctcgagg ccctgccgct gtccgacgag ccagcggtgg
     5521 tgaccgtcgg gttcttcgac ggggtccatc tgggacacca acaggtgttc gccaccacgg
     5581 tgaagcgagc ccgcgaacgg ggcatccggt cggtggcggt cacgttcgac cggcacccac
     5641 gggaggtgct gacgcccggc caggaacccc ggctgctcac caccgtggag cgcaaggcat
     5701 cgttgatcga ggcgatgggc atcgacgtgc tggtggtgct cccgttcacc gcggcgttct
     5761 cgcgggtccc ggcggaggac ttcgtccggg acgtgctggt gggcggcatc cacgcggtac
     5821 acgcggcgat gggcgcgaac ttcacgttcg ggttcaaggc ggcgggcacc gtcgagaccc
     5881 tgccggcgat gggggagccc tacggcctca ccgccgaggg cgtcgcgctg ttcgagctgg
     5941 acggccgaac ggtgtcctcg tcctcgatac gggatgcgtt gtcggccggt gatctggcat
     6001 ggccgctcgc ggcgttgggc cgacggttcg tgctggacgg cgaggtcacc tccgggcatg
     6061 gccgtggcaa ggggctgggc tatcccaccg cgaacctgcg cacgtggccg cggctgatgc
     6121 tgcccggtca ggggatctac gccggcgtgg ccgaggtcga cgggctccgc tacccggcgg
     6181 cgatcgacgt cggcaccaac cccacgttcg gcgtggagcc gctgcacgtc gaggcgttcc
     6241 tgctcgactt cgagggggag gaactgcgag gcaagcccat ctcgatcgag ttctgggagc
     6301 gcctgcgcga tgagatcaag tacgactcgg tggaggcgtt gatcgaggcg atcgcggtcg
     6361 acgtccaacg tacccgcgag ctggtcgacc cttcggcctg agctacccgt cgtcggtccg
     6421 cagcagccgg atcgccaggc gcgccatcag cagcagcggg atcgtgacgc cggcgcgttg
     6481 caccagtccg ttgcccggcc gcgtcacgcc gcgtgcgaac cacgcgagca accccgagcc
     6541 ggcggcggcc gctaccatcg tggggcgcgc gagatcctcg aggcccggtt cgtgccggag
     6601 ccgggccccc agggcgaaca atcccaccga cgaggtgagc gccccgacga ccgacgccgc
     6661 gtcgtggaca tcgttgatcc ccgactggcc cggagcctgg ccgggttcgg gatggttgga
     6721 catccggtcg cgccggaaca gccccgccac gaccatcgcc acgcccgacg caccgatcag
     6781 cgcggggccg aggccgggag atgccttgac cgatcccttg c
//
LOCUS       DATDBZ010000261         3930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2741737, whole genome shotgun sequence.
ACCESSION   DATDBZ010000261 DATDBZ010000000
VERSION     DATDBZ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741737"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VIX62_06405"
     CDS             complement(<1..1038)
                     /locus_tag="VIX62_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859896.1"
                     /translation="MISGRQQIRPDAAVRGLFVTSGFVIAAFFPFLALYLDGKGLSES
                     EIGLVIAAMAVARIVLNPFWGHVSDSVLGRRTSLQIGLVLAAGAALFLASAHDLWVIA
                     TAGFLLAGAMVTLGPNIDAITLEHLGADRMSEYGKIRSWESFTYAIGCLVLGALFQAS
                     GVEWAMPIYAIASLGVLAWSFVVARDAPKHESSGDGKLGAIGAVFRAAPRFWGFLAAL
                     FLVWTGFNAAWNFISLKIASEGGGPWLVGIGTALGGLIEVPMMRSSSRLQARFGLRRV
                     YALGCLIYATGFLLWGLVPNPTIVSLLTVLEGMAFSLLFTTGVVVVGRLLPSSLYSSG
                     NSVAQMVGFGLG"
     gene            complement(1035..2105)
                     /locus_tag="VIX62_06410"
     CDS             complement(1035..2105)
                     /locus_tag="VIX62_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5859897.1"
                     /translation="MTDHEDLRELRLRRFRPVAALRTAQHFVPRARFPAIDVHNHLGR
                     WLTSGWAAADVGALLEVMDAANVVAIVNLDGMRGEELQANLDRYDRAHPGRFVTFAQV
                     DWHEVARGAGFGERLAAQLREAVDAGARGLKVWKDLGLHLRDDAGVLIAPDDERLSPL
                     WEMAGDVGVPVLIHTGDPVAFFDPMDERNERIEELLEHPDWWFGDRARFPAFDDLLGA
                     FERLVASHPRTTFIGAHVAGAAEDLGWVGPMLETYPNLHVDLAARIAELGRQPRAIRA
                     LLERHPTRILFGTDEFPPMQEVYAIHFRFLETADESFAYSIEDPPPQGRWAISGLDLP
                     DDVLRAVYRENALRLIPGLDPS"
     gene            complement(2102..2860)
                     /locus_tag="VIX62_06415"
     CDS             complement(2102..2860)
                     /locus_tag="VIX62_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY5859898.1"
                     /translation="MRQSERLSAILEALANDGSVGVADLATQLDVSAATVRRDLEFLE
                     HQRMLTRTHGGAVAQGVLYELPLRYKSARHQEEKKRIAAEAATRVADGVAIGLTGGTT
                     TTEVARAVIDRQRLTVVTNALNIASELAIRPNLKLVVTGGHARPESYELVGPLAEQAL
                     AGLNLDVVFLGVDGISPTAGITTHHEVEAHTNLALIERARHVVVVTDSSKIGLVAFAQ
                     ICPIDRVHEVITDAGADRGLLAELREAGVDVVTV"
     gene            2933..3805
                     /locus_tag="VIX62_06420"
     CDS             2933..3805
                     /locus_tag="VIX62_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HEY5859899.1"
                     /translation="MSETEREIASQPSCWERAQTLASEIASRLPAPRERVAAVGCGTS
                     WFMAQAYAALREGSGVGETDAFTASEFPPDRSYDRIVAISRSGTTTEVVRLVEQVTAP
                     TTAITAVAGTPIPELATDAVVLDFADERSVVQTRFATTTLALLRAHTGQDLTAAIAQG
                     EQAAVADLAVDATAFDHFVFLGHGWTVGLANEAALKMREAAQAHTEAYPAMEYRHGPI
                     SLAGPGSLVWVLGTPDPAVAEDVRATGATVMVAEDDPMAELIRIQRSAVAVATARGLD
                     PDHPRNLTRSVVLS"
BASE COUNT          641 a         1389 c         1336 g          564 t
ORIGIN      
        1 cccgagtccg aacccgacca tctgcgccac ggagttcccg ctcgagtaca gcgacgacgg
       61 caggagcctt ccgaccacga ccaccccggt ggtgaacagc aggctgaacg ccatgccttc
      121 caggacggtg aggagcgaga cgatcgtcgg gttcggcacc aggccccaca gcaggaagcc
      181 cgtcgcgtag atcaagcacc cgagcgcgta cacccggcgc agcccgaacc tcgcctgcaa
      241 ccgtgaggac gatcgcatca tgggcacctc gatcagcccg ccgagcgccg tgccgatccc
      301 caccagccac ggtccgccgc cctcgctcgc gatcttcagc gagatgaagt tccacgcggc
      361 gttgaacccg gtccacacca ggaacagcgc cgccaggaac ccccagaacc gtggagcggc
      421 ccggaacacc gcgccgatcg cgccgagctt gccgtcgccg gaggattcat gcttcggagc
      481 gtcccgcgcc acgacgaacg accaggccag caccccgagg ctggcgatcg cgtagatcgg
      541 catcgcccac tccacgcccg aggcctggaa gagcgcgccg agcaccaggc aaccgatcgc
      601 gtaggtgaag ctctcccacg accggatctt gccgtactcc gacatccggt cggcgcccag
      661 gtgctccagc gtgatcgcgt cgatgttggg acccagcgtc accatggctc cggccaggag
      721 gaaccccgct gtcgcgatca cccacaggtc atgcgcgctc gcaaggaaca acgcggcgcc
      781 ggccgcgagc accaatccga tctgcaacga ggtccgccgg ccgagcacgc tgtcggacac
      841 gtggccccag aaggggttca gcacgatgcg cgccaccgcc atggccgcga tcaccagccc
      901 gatctcgctc tcgctcaggc ccttgccgtc caggtacagc gcgaggaagg ggaagaacgc
      961 ggcgatcacg aatccggagg tgacgaacag cccccggacc gcggcgtcgg ggcggatctg
     1021 ttggcgaccg ctgatcacga cgggtccagc ccggggatca accgcagcgc attctcgcga
     1081 tagaccgccc gcaggacgtc gtccggcagg tcgaggcccg agatcgccca ccggccctgc
     1141 ggcggtggat cctcgatcga gtacgcgaac gactcgtcgg ccgtctccaa gaaccggaag
     1201 tggatcgcgt agacctcctg catcgggggg aactcgtcgg ttccgaacag tatgcgggtg
     1261 gggtgtcgtt ccagcagggc gcgtatggcg cgcggttggc gacccagttc ggcgatgcgc
     1321 gccgcgagat cgacgtgcag attggggtag gtctcgagca tgggcccgac ccaccccaga
     1381 tcttccgcag cgcccgcgac gtgcgccccg atgaaggtgg ttcgcgggtg actcgccacc
     1441 agccgctcga acgcccccag caaatcgtcg aacgccggga accgagcgcg gtcgccgaac
     1501 caccagtcgg gatgctccag cagttcctcg atgcgttcgt tgcgctcgtc catgggatcg
     1561 aagaacgcga ccgggtcgcc ggtgtggatc agcaccggga ccccgacgtc gccggccatc
     1621 tcccacaaag gcgacagccg ctcgtcgtcg ggcgcgatca acacgccggc gtcgtcgcgc
     1681 aggtgcaatc cgaggtcctt ccagaccttg aggcctcgag caccggcgtc aacggcttca
     1741 cggagctgcg cggccaaccg ttcaccgaac ccggcgccgc gcgccacctc atgccagtcc
     1801 acctgcgcga acgtgacgaa ccgtcccgga tgcgcccggt cgtagcggtc gaggttggcc
     1861 tgcagctcct cgccgcgcat cccgtcgagg ttcacgatcg cgacgacgtt ggcggcatcc
     1921 atcacctcga gcagcgcacc cacgtcggcc gcagcccagc cggacgtgag ccaccgaccc
     1981 aggtggttgt ggacgtcgat cgcggggaac cgtgcccgtg gaacgaagtg ttgggcggtc
     2041 cggagcgccg ccacgggccg gaatcggcgc agccgaagct cccgcaggtc ctcgtggtcg
     2101 gtcacacggt caccacgtcg acgccggcct cgcgcagttc ggcgagcaga ccgcgatcgg
     2161 cccctgcgtc ggtgatcacc tcgtggacgc gatcgatcgg gcagatctgc gcgaaggcga
     2221 cgaggccgat cttcgagctg tccgtcacga cgacgacgtg gcgagcgcgt tcgatcaggg
     2281 ccaggttcgt gtgcgcctcc acctcgtggt gggtggtgat gccggcggtg ggcgagatgc
     2341 cgtccacccc gaggaacacg acatccaggt tgagtccagc gagcgcttgt tcggcgaggg
     2401 gacctaccag ctcgtaggac tcggggcgcg cgtgcccacc ggtgacaacc agcttcaggt
     2461 tgggacggat cgcgagttcg ctcgcgatgt tcagggcgtt cgtgaccacg gtgaggcgtt
     2521 gacggtcgat caccgcgcgc gccacctcgg tggtggtggt gccgccggtg agcccgatcg
     2581 cgacgccgtc ggcgacgcgc gtggccgcct cggcggcgat gcgcttcttc tcctcctggt
     2641 ggcgcgcgct cttgtagcga agcggcagtt cgtacagcac gccctgggcc accgcacccc
     2701 cgtgggtgcg cgtgagcatg cgctggtgct cgaggaactc gaggtcgcgg cggacggtcg
     2761 ccgccgagac gtcgagctgg gtcgcgagat cggccacgcc cacggagccg tcgttcgcca
     2821 gggcctcgag gatggcgctg agccgctccg actggcgcat gagccggagt ataggcgtcg
     2881 catgcacggt tggattgcgt ttctgttcgt ttggagcgta tggtgcgcag gaatgagcga
     2941 gactgagcgg gagatcgcga gccagccctc gtgctgggag cgggcgcaga ccctcgcgtc
     3001 cgagatcgcc tcacggctgc cggcgccccg tgaacgcgtc gccgctgtgg ggtgcggcac
     3061 ctcctggttc atggcgcagg cgtacgcggc gcttcgagaa gggtccggtg taggcgagac
     3121 cgacgccttc accgcctcgg agttcccacc cgaccgctcc tacgaccgca tcgtggcgat
     3181 cagccggagc ggcaccacca ccgaggtggt acggctggtc gagcaggtca cggcgccgac
     3241 caccgcgatc accgccgtgg cgggcacgcc gatcccggaa ctcgccaccg acgccgtggt
     3301 cctggacttc gccgacgaac ggtcggtggt gcagacccgg ttcgccacga ccacgctcgc
     3361 gctcctgcgc gcgcacaccg gccaggacct caccgccgcg atcgcccagg gtgagcaggc
     3421 cgccgtcgcc gacctcgccg tcgatgccac cgcgttcgat catttcgtgt tcctggggca
     3481 cggatggacg gtgggactgg ccaacgaggc cgccctgaag atgcgtgagg ccgcgcaggc
     3541 ccacaccgag gcctatcccg ccatggagta ccggcacggg ccgatcagcc tggcgggacc
     3601 aggttcgctc gtctgggtgt tgggaacgcc cgaccccgcg gtcgccgaag acgtgcgagc
     3661 gaccggcgcc acggtcatgg tcgccgagga cgaccccatg gccgagctca tccggatcca
     3721 acgttccgcc gtggctgtgg ccaccgcccg gggcctcgat ccggaccatc cccgcaacct
     3781 gacccgatcc gtcgtgctgt cgtgatgcgt aggaggaaga agatgaagcg tcgatccctg
     3841 ttcgtgctct tcaccgtgat ggcgttggtc gccgccgcgt gctcgggcgg cgaggacggc
     3901 tccaccgacg cctcggattc cggcggcggc
//
LOCUS       DATDBZ010000262         2818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1089132, whole genome shotgun sequence.
ACCESSION   DATDBZ010000262 DATDBZ010000000
VERSION     DATDBZ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089132"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VIX62_06425"
     CDS             <1..214
                     /locus_tag="VIX62_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859900.1"
                     /translation="QGLLGGIETLVAGVTALAAGALYEEFDRTVAYTACAIAMVTCVL
                     VAYALVGPEWRRRPATVEMVSAGSRH"
     gene            complement(410..1639)
                     /locus_tag="VIX62_06430"
     CDS             complement(410..1639)
                     /locus_tag="VIX62_06430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5859901.1"
                     /translation="MEFGVFLNGYIPGPAAHDTMCEHTALMREAEYAIVADRHRWKYV
                     WFGEHHGLTEYSHMSAPEVMMGYVAARTERIHLGSAITSLPTRKEHPVRIAERAAMLD
                     HITGQRFEFGTGRGAGSHEVAMFNVLDTNDTKAQWDEVIHEIPRMWEQRDYDFSGDHF
                     TVPTPHNVLPKPYGLGHPPLWVAGGNPPTFAKAGAHGIGAIAFNFEPTHNLKGRVESY
                     KEAIQHPTDQIGQYRNDNVMMTNAVVCLNDRRRAREIALTAGRGYLVTMVNLYHDTMP
                     KSPNARTWPDPPIVLPHDEDLLDQLIAGGYMLCGTPEEVCEQLVRYQAVGCDQVVFGL
                     PGEGMHHDEILEQLEVFGEQVIPEFDLDPVHSTTRYREQAQRRFPDFAHPVPDVSVSL
                     LPDTALTPARAPAPAPT"
     gene            complement(1717..2403)
                     /locus_tag="VIX62_06435"
     CDS             complement(1717..2403)
                     /locus_tag="VIX62_06435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020038847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5859902.1"
                     /translation="MTESQPIGLDDAYALESPEDSRRLYARWADTYDSGFVQPLDYRY
                     PERVAAVFVDVARELDGPILDVGCGTGLVGLALADAATGDVHIDGIDISAAMIDRAAR
                     RQRADGSPVYHRLIEADLTQPIDIPDGTYAGLVSAGTFTHGHLGPDDLGGLLRLGRPG
                     AVFVIGINAEHYGERGFAARLDTDVLSGAIEALELRQIPIYGPDSPRADDLAMIAVFR
                     RGRTGAASST"
     gene            complement(2420..>2818)
                     /locus_tag="VIX62_06440"
     CDS             complement(2420..>2818)
                     /locus_tag="VIX62_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859903.1"
                     /translation="LSLTLGGCAVIAFVIGLSSWWPVVFVMFTAVSLLGILWNVITVS
                     LRQTIIPPHLLGRVNSVYRFFAWGTLPVGAALGGLVVIVVDTFASRDVALRATWIVNG
                     AIHVLLFVFGRRKLTTARIEAARTAERVVD"
BASE COUNT          420 a          913 c         1011 g          474 t
ORIGIN      
        1 gcaaggcttg ctcggtggca tcgagacgct cgtcgccggg gtcacggcgc ttgcggccgg
       61 ggcgctgtac gaggagttcg atcgcaccgt cgcctacacc gcatgcgcga tcgccatggt
      121 gacgtgtgtc ctcgtcgcct acgcgctggt cggcccggag tggcggcgcc gcccggccac
      181 ggtcgagatg gtgtcagcgg gctcgcgcca ctgatctgat ggatcgggac tggccgttcc
      241 gatggatcgg gactggccgt tccgatggat cgggactggc cgccgcttcg cttcggccgc
      301 tcccgatcgg ctcgctgcgc ctggcggcgc cacgctcgtg tttgtgcgct cggttgcgga
      361 tagcgcaacc tcgtgcgcaa acgctcggca cgatggggtc aggcggcggt caggtgggtg
      421 ctggggcggg tgctcgtgcg ggggtgagcg ccgtgtcggg cagcaggctg acggacacat
      481 ccggcaccgg gtgggcgaag tccgggaacc ggcgctgcgc ctgctcgcga taccgggtcg
      541 tcgagtgcac gggatcgagg tcgaactcgg ggatgacctg ctcgccgaag acctcgagct
      601 gctcgaggat ctcgtcgtgg tgcatgccct caccgggcag cccgaacacg acctggtcgc
      661 acccgaccgc ctggtaacgc acgagctgct cgcacacctc ctccggtgtg ccgcacagca
      721 tgtagccgcc ggcgatgagc tgatcgagga ggtcctcgtc gtgcgggagg acgatgggcg
      781 gatcgggcca ggtgcgggcg ttcggcgact tcggcatggt gtcgtggtag aggttcacca
      841 tcgtgacgag atagccgcgg ccggccgtca gcgcgatctc gcgcgcccgg cggcgatcgt
      901 tcaggcagac gactgcgttc gtcatcatca cattgtcgtt ccggtactgg ccgatctggt
      961 cggtcgggtg ctggatcgcc tccttgtacg actcgacccg ccctttcagg ttgtgggtcg
     1021 gctcgaagtt gaacgcgatc gcgccgatgc cgtgggcccc cgctttggcg aacgtgggtg
     1081 ggttgccgcc cgccacccac agcggcggat ggccaaggcc atagggcttc ggcaggacgt
     1141 tgtgcggtgt cggcacggtg aagtgatcgc cgctgaagtc gtagtcacgc tgctcccaca
     1201 tgcgcgggat ctcgtggatc acctcgtccc actgcgcctt cgtgtcgttc gtgtcgagga
     1261 cgttgaacat cgccacctcg tggctgccgg caccacggcc ggtgccgaac tcgaagcgct
     1321 gcccggtgat gtgatcgagc atcgctgctc gctccgcgat gcgcacggga tgctccttgc
     1381 gcgtgggcag gctcgtgatc gccgacccga ggtggatgcg ttcggtgcgc gccgcgacgt
     1441 accccatcat cacctcgggg gcggacatgt ggctgtactc cgtcagcccg tggtgctcac
     1501 cgaaccacac gtacttccag cggtggcggt cggcgacgat cgcgtactcc gcttcccgca
     1561 tgagcgcggt gtgctcgcac atggtgtcgt gcgccgcagg acccgggatg tagccgttga
     1621 gaaagactcc gaactccacg acgcgcctcc gattgggccg aggggacacc ccactggtgg
     1681 tccgccgaac gtagcaggtt ctaatgcatc cgattactac gtcgacgagg cggcacccgt
     1741 ccggccgcgc cggaacacgg cgatcatcgc gaggtcgtcg gcgcgtgggc tgtcgggtcc
     1801 gtagatcggg atctgtcgga gctcgagggc ttcgatcgcg ccggacaaca catcggtgtc
     1861 gagccgcgcc gcgaaccctc gctccccgta gtgctctgcg ttgatgccga tgacgaacac
     1921 ggcgcccggc cgtcccaacc gcaggaggcc gccgagatcg tccgggccga gatgcccgtg
     1981 ggtgaacgtg ccggcgctga cgaggccggc atacgtgccg tcagggatgt cgatcggctg
     2041 cgtgaggtcc gcttcgatga gccggtggta gacggggctg ccgtcggccc gctgccgtcg
     2101 agcagcccgg tcgatcatcg cggcggagat gtcgatgccg tcgatgtgga cgtcgccggt
     2161 cgcggcgtcg gcgagtgcga ggccgacgag gccggtgccg caaccgacgt cgaggatcgg
     2221 cccgtcgagc tcgcgggcga cgtcgacgaa caccgcggcg acccgttccg ggtagcggta
     2281 gtcgagcggt tgcacgaagc cgctgtcata cgtgtcggcc catcgggcgt agaggcggcg
     2341 gctgtcctcg ggtgactcga gcgcgtatgc gtcgtccagg ccgatgggct gggattcagt
     2401 cacgggcgta ccccctcgat cagtcgacga cgcgctctgc agtgcgggcc gcttcgatcc
     2461 ttgccgtcgt cagcttccgc cgtccgaaga cgaagagcaa gacgtggatc gcaccgttga
     2521 cgatccacgt ggcccgcagc gcgacgtcgc ggctggcgaa ggtgtcgacg acgatcacga
     2581 ccagcccgcc gagagcggcc cccaccggca acgtgcccca ggcgaagaag cggtagacgc
     2641 tgttcacccg gccgagcagg tgtggcggga tgatggtctg acgcaggctg acggtgatga
     2701 cgttccacag gatgccgagc agcgagaccg cagtgaacat cacgaacacc accggccacc
     2761 agctcgacaa cccgatcacg aatgcgatca ccgcgcagcc gcccagggtc aggctgag
//
LOCUS       DATDBZ010000263         3884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1090159, whole genome shotgun sequence.
ACCESSION   DATDBZ010000263 DATDBZ010000000
VERSION     DATDBZ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090159"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VIX62_06445"
     CDS             <1..1259
                     /locus_tag="VIX62_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY5859904.1"
                     /translation="AMPPKERAEVLHRIADGVEARVPELAAVETRDNGSLLRSMRNSV
                     MPRVARNFRFFADQLLALETGVKDYDGFAERVAWDPSGVTVVITPWNAPLMLATWRIA
                     PALAAGNAVILKPPEWAPLTASMLADIAHEAGLPDGAFNVVQGIGEEAGAALTAHPGV
                     DRIAFTGSVPTGKLVAQAAGANLAPVSLELGGKSPFVAFADADLDAVVQQAVNQFDNA
                     GQVCLAGTRLIVERSMHDEFLERFVEASAAIVQGDPREETTDIGPQITRAHFERVDGF
                     VQRAKTDGATVVFGGGPNEDLGGLYYRPTLFTDAPSGSEILTQEVFGPVLTLQTFESE
                     DEAVALANSTEYGLAAIVYTGDQARAERVSSALVAGTVWVNCFFVRDLAAPFGGARGS
                     GIGREGGSWSFDFYADVKNVCTAPWN"
     gene            1284..2183
                     /locus_tag="VIX62_06450"
     CDS             1284..2183
                     /locus_tag="VIX62_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HEY5859905.1"
                     /translation="MGEVVGAALVAHVPTIMLPEETRREINDGNEITLVPGLHRLKSE
                     CLDRLQPDTVIVFDTHWESTFEHIVTAQERRTGKFTSHELPRGMAGIPYDMPGDPELA
                     NLIAAQAEGRDDCWILACDDPYLPIFYGTVNVWTFLGDANTRWISVALNQCCTTEDFL
                     LFGKLIGDAIAQSDRRVVLLASGGMTHRFFPFRELRQHESSKAPENILGIESYEADQR
                     VLSMMRDGDHAGVIDWMPEYRKAAPEGHFGHYLMMASAIGGDRCQAPGELYSEYEASV
                     GTGQVHVWFERPADGWTGKGAAA"
     gene            2183..3688
                     /locus_tag="VIX62_06455"
     CDS             2183..3688
                     /locus_tag="VIX62_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY5859906.1"
                     /translation="MGGRTEKGGSTVAEQQTQEMGYERLGKARLSLVDVLAQSVGFMG
                     PVFSAAFIIPLIIGVNFAARGAGTSAVVSVLLAAVGVFAIGWIVAQYAKRIHAAGSLY
                     DYVSEGLGKTLGAAGGWLYYAGTIILTVGLGVLLGGFIKDTLLIAFEIDLDLPIWVWD
                     AVVAGLLLAVLYLGVQISTRVQLALALISIAVVLVFFATVISKLGDANDFGKALDPSP
                     DGGFSGILFGVLYGVLIFVGFETAANLAEETAEPQKSIPRAVLGAVVLVSIFYVIAAY
                     VEVAGFGFDLAVITSAEVAGAPLFALGAPGGEFGSEFWLKILILVVFLDMLAVYVGAG
                     VASTRGMFAMARDRRLPAPLASVSKRFGTPAGAIALLIVIQGLLIVASEWTEVFALPG
                     LPHYFALFAWCSTFGGFALLVVYLMMSVGALASLGRQAKPIGVAIASILGILITAAAI
                     WGSFYKVPTPTVWAPRYALIWFVIGLVYMFLVKGREPASQALADLSTKDVA"
BASE COUNT          563 a         1300 c         1358 g          663 t
ORIGIN      
        1 gcgcgatgcc cccgaaggaa cgcgccgagg tgttgcaccg catcgccgac ggcgtcgagg
       61 cgcgcgtgcc cgagctcgca gcggtggaga cgcgcgacaa tggttcgctg ctgcgctcga
      121 tgcgcaactc cgtgatgccc cgggtcgctc gcaacttccg gttcttcgcc gatcagcttc
      181 tcgcgctcga gaccggggtg aaggactacg acgggttcgc cgaacgcgtg gcctgggacc
      241 cgagcggggt cacggtggtc atcacgccct ggaacgcacc gctcatgctc gcgacgtggc
      301 gcatcgcgcc cgcgctcgcg gcgggcaacg cggtcatcct gaagccgccg gaatgggcgc
      361 cgctcacggc ctccatgctc gccgacatcg cgcacgaggc cggtctgccc gacggcgcgt
      421 tcaacgtcgt gcagggtatc ggtgaggaag cgggcgccgc cctcaccgcg cacccgggcg
      481 tcgatcgcat cgcgttcacg ggctcggtgc cgacgggcaa gcttgtcgcc caggccgcgg
      541 gcgccaacct cgcgccggtc tccctcgagc tcggcggcaa gtcgccgttc gtcgcgttcg
      601 ccgacgccga cctcgacgcc gtcgtgcagc aagccgtgaa ccagttcgac aacgccgggc
      661 aggtctgcct cgccggcacg cgcctgatcg tggaacgcag catgcacgac gagttcctcg
      721 agcggttcgt cgaggcttcc gcagcgatcg tgcagggcga cccccgcgag gagaccaccg
      781 acatcggtcc gcagatcacc cgggcgcact tcgaacgggt cgacgggttc gtgcagcgag
      841 cgaagaccga cggcgccacg gtggtcttcg gcggcgggcc caacgaggac ctcggcggcc
      901 tgtactaccg gccgaccctg ttcaccgatg cgccgagcgg gtcggagatc ctcacgcagg
      961 aggtcttcgg ccccgtgctc acgctgcaga ccttcgagag cgaggacgag gccgtcgcgc
     1021 tcgccaactc gaccgagtac gggctggccg ccatcgtcta caccggcgac caggcccgcg
     1081 cggaacgcgt gagctccgcg ctcgtggcgg gcaccgtgtg ggtcaactgc ttcttcgtgc
     1141 gcgacctcgc ggcgccgttc ggtggcgcgc gcggctcggg catcggccgc gagggcgggt
     1201 cgtggagctt cgatttctac gccgacgtga agaacgtgtg caccgcaccg tggaactgac
     1261 accgagaccg aggaggcaac gacatgggtg aagtggtcgg cgcggccctg gtggcccacg
     1321 tgcccaccat catgctgccc gaggagacgc gccgtgagat caacgacggc aacgagatca
     1381 cgctcgtgcc cggcctgcac cggctgaaga gcgagtgcct cgaccggctg cagcccgaca
     1441 ccgtgatcgt gttcgacacc cactgggagt cgaccttcga gcacatcgtc acggcgcagg
     1501 agcgccgcac cgggaagttc acgagtcacg agctgcctcg aggtatggcg ggcatcccgt
     1561 acgacatgcc cggcgacccc gagctcgcga acctcatcgc ggcccaggcc gaggggcgcg
     1621 acgactgctg gatcctcgcc tgcgacgacc cgtacctgcc gatcttctac ggcacggtca
     1681 acgtgtggac gttcctcggc gacgcgaaca cgcggtggat cagcgtggcg ctgaaccagt
     1741 gttgcactac cgaggacttc ctgctgttcg gcaagctcat cggcgacgcc atcgcgcaga
     1801 gcgaccgccg cgtcgtgctg ctggcgagcg gcggcatgac gcaccgcttc ttcccgttcc
     1861 gggagctgcg ccagcacgag agctcgaagg cgcccgagaa catcctgggc atcgagagct
     1921 acgaggccga ccagcgcgtg ctctcgatga tgcgggatgg cgaccacgcc ggggtcatcg
     1981 actggatgcc cgagtaccgc aaggccgcgc ccgaggggca tttcggccac tacctgatga
     2041 tggcgtcggc gatcgggggc gatcgttgtc aggcgcccgg cgagctgtac agcgagtacg
     2101 aagcatccgt cggcacgggc caggtgcacg tctggttcga acgcccggcc gacggctgga
     2161 ccggcaaggg ggcggccgcc tgatgggcgg ccgtacggag aaggggggct ccacggtggc
     2221 ggaacagcag acacaggaga tgggttacga acgccttggc aaggcgaggc tttccttggt
     2281 ggacgtgctc gctcaatcgg tcggtttcat gggaccggtg ttctcggcgg ccttcatcat
     2341 cccgctgatc atcggcgtga acttcgcggc gaggggcgcc ggcacctcgg ccgtcgtgtc
     2401 ggtcttgttg gctgccgtgg gcgtgttcgc catcggttgg atcgtcgcgc agtatgcgaa
     2461 gcgcatccac gctgcgggat cgctctacga ctacgtgtcg gagggcctcg ggaagacctt
     2521 gggcgccgcc ggtggatggc tctactacgc cggcacgatc atcttgacgg ttggcctcgg
     2581 tgtgttgctc ggcgggttca tcaaggacac cctgctcatc gcgttcgaga tcgacctcga
     2641 cctgccgatc tgggtctggg acgccgtcgt ggctgggttg ctgctcgcgg tcctctacct
     2701 gggcgtgcag atctcgacca gggtgcagct cgcgttggca ctgatctcga tcgcggtggt
     2761 gctcgtcttc ttcgccaccg tgatcagcaa gctcggtgat gcgaacgact tcgggaaggc
     2821 cctggacccc agtcccgatg gaggcttctc gggcatcttg ttcggcgtcc tctacggggt
     2881 cctgatcttc gtgggattcg agactgccgc gaacctggca gaggagacgg ccgagccgca
     2941 gaagtcgatc ccgagggccg tgctcggggc ggtcgtgctg gtgtcgatct tctacgtgat
     3001 cgccgcctac gtggaggtcg cgggtttcgg gttcgacctg gcggtcatca cgagcgccga
     3061 ggtcgccggg gcgccgctgt tcgccctggg ggcgccggga ggcgaattcg gctccgagtt
     3121 ctggctgaag atcctgatcc tcgtcgtctt cctcgacatg ctggcggtct acgtgggcgc
     3181 gggtgtggca tcgacccgcg gcatgttcgc gatggcgcgc gatcggcgct tgccggcgcc
     3241 tctggcttcc gtgtcgaagc gattcggcac gccggcgggg gcgatcgcac tcctgatcgt
     3301 gatccagggg ctcctgatcg tggcgagcga atggaccgag gtgttcgccc ttccaggtct
     3361 cccgcactac ttcgcgctgt tcgcgtggtg ctcgacgttc ggcgggttcg cgctcctcgt
     3421 cgtgtacctg atgatgtccg tcggggcgct cgcttcgctc ggccgtcagg cgaagcccat
     3481 cggggtggcg atcgcgtcca tcctgggcat cctcatcacg gcagccgcca tctggggctc
     3541 cttctacaag gtgccgacac cgacggtgtg ggctcctcgg tatgcactga tctggttcgt
     3601 gatcggtctc gtctacatgt tcctcgtgaa gggccgggaa cccgcgagcc aggcgctcgc
     3661 cgatctgagc acgaaggatg tggcgtaacg ctccgccatt cgctcacatg actgacgaac
     3721 gaccgctgcg gctcgatgcg cctcgcgacg aggcgctcga gcacgcggcg cgcctggtcg
     3781 ccgaggcctg gcgcagcttc gacaccttcc ggcccgagga gccgccgctc gacgatcgcg
     3841 tgcggcggct cctcgagggc gcgctgcccg ccgcgccctc gccc
//
LOCUS       DATDBZ010000264         4943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_702465, whole genome shotgun sequence.
ACCESSION   DATDBZ010000264 DATDBZ010000000
VERSION     DATDBZ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702465"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VIX62_06460"
     CDS             <1..1072
                     /locus_tag="VIX62_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859907.1"
                     /translation="SRPADAARRAARVGSSCMGIPVRRTVTACVLATSCLVSASAPLT
                     VLAEPVPEAARCPAYDRTWQNAGLSGAGASVLNELSGIQASLAHRGVLWSVEDGNNGP
                     HLFAFNAGGEVIGDFTLSKADVGNLDWEALGLDRRKGRDQLYIGDIGDNAHARDGSGQ
                     KVPALYRIAEPKIRAGASGVTRTITRIKKFTFRYYTKAGVLAPRNAESMFVDPRRHDV
                     IVITKDLETIGGHANRVRAFRMRVGALHGGRLNHAKQVATLTAATDGHGVGAVSADIA
                     RDGRWIVVKNYGQGFLWHRDPHGPAWSAFRRHPVAPCRVPVDSAEAISFSYTDGGRWN
                     GFWSVTETGSPPPPLRHLVGPD"
     gene            1131..1478
                     /locus_tag="VIX62_06465"
     CDS             1131..1478
                     /locus_tag="VIX62_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859908.1"
                     /translation="MDESEIAELRARVAEAERERDAAEARAQAAETRAEDLGALDDRV
                     QVAERRALDAERRLQELSEQVQASRPGTDTSEEPLEPVGSTETAPPAANDLRARLART
                     AARKKPGGGPDPT"
     gene            complement(1483..2064)
                     /locus_tag="VIX62_06470"
     CDS             complement(1483..2064)
                     /locus_tag="VIX62_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859909.1"
                     /translation="MNRTSTASRAGGLELAAGAEPILAWRAWALTGTRDGDDLMLRPV
                     ARRSRPWKPRQPAEAACSLARFHRAPHAGCTCGLHGTHAVEILRKTKCPAVLGRVALW
                     GTVIEHEYGYRARFAYPQRTRLICQFCFWMAGPYAAAPEHVGWFPKDELMPICDEHLE
                     TASRYGLAPRHVLPAEAVDQRLRETYAVDALAV"
     gene            complement(2061..2225)
                     /locus_tag="VIX62_06475"
     CDS             complement(2061..2225)
                     /locus_tag="VIX62_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859910.1"
                     /translation="MDIGQPKRIIEITPAAIPVPGELVPDLAPDMTPEREPEREPQPA
                     GPESADRRSR"
     gene            complement(2267..3379)
                     /locus_tag="VIX62_06480"
     CDS             complement(2267..3379)
                     /locus_tag="VIX62_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY5859911.1"
                     /translation="MRTIRTTVRLLILTLIASSLAVVTLAPAANAGASIKVLKVKGGL
                     RGPAGFTFLPGGKLLYAERGSGQIRILNLETKHTRRFFTVSGVNGAGERGALGVAVHP
                     AWPDQPFVYVFATRNAGGNLRNQIVRIRANKHDGAWKGVGMKVLLSAPASNDPYHNGG
                     RIEFGPDGRLYAIVGDGHNSTNAQDVTGNLRGKILRLRADGGVPADNPTIGGERTRMF
                     AYGIRNSFGFAFDPDTGDLWETENGPGCNDEVNRIVAGENYGWGPSESCPNTNNSGPS
                     PRLPLVTYDSTIGITGAVFCDGCGLAGAEGDLFWGACCDGGSLHRGVLNGPRDDFANV
                     VTVFDVPGGSIYSMERAPNGRIYFSDSSAIYRLAPV"
     gene            complement(3412..4488)
                     /locus_tag="VIX62_06485"
     CDS             complement(3412..4488)
                     /locus_tag="VIX62_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY5859912.1"
                     /translation="MTGIVALAGSPAATAAPPKVRVVPVATLANPVGFTFTPDGRIVY
                     AERDTGEVRFLNPRTGSDRLFFRIGGVNSEGERGALGVALHPDWPRTPFVYVYVTRGP
                     GGEPIRNQLIRLRAVHGHGRNFTVLLQSPIGSRANHNGGRIAFGPDGKLYVVIGDGGE
                     DPSTAQDLTDEPRGKILRLNPNGSVPPSNPFPGSTVYSFGHRNSIGFAFDPDSGTLWE
                     TENGPDCNDEINRIQAGGNFAWGPSQACPDTNQDGPDPKLLPVWNFDETVAVTGTAFC
                     DGCGLGAAYDGDLFVGCANGTCKQTVGPVARANLNGARDDFEGALQKVALVNFAGPVY
                     SMETSPNHRLYFSNALGIYRLAPA"
     gene            4487..>4943
                     /locus_tag="VIX62_06490"
     CDS             4487..>4943
                     /locus_tag="VIX62_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859913.1"
                     /translation="MSTAVIRTVPVRVLRMCFSPSDDRWWPHALWEHEVVPTLPPSMF
                     GQPALRSEDPRFLTGRGRYLENLPIEGVLRAVFVRSMMPHARVIAVDVDAARAMPGVV
                     AVYTADDLAIRAMPPSGNVEGASGTLEGLFGREVMAREVVRFVGEPLALV"
BASE COUNT          708 a         1697 c         1760 g          778 t
ORIGIN      
        1 cagccgtccg gccgatgcgg cccgtcgcgc cgcgcgggta ggctcctcgt gcatggggat
       61 cccggtccgg cgaacggtga cggcgtgtgt cctggcgacc tcctgcctgg tctcagcgtc
      121 tgccccgctc accgtcctcg ccgagccggt tcccgaggcc gccagatgtc ctgcctacga
      181 ccgcacctgg cagaacgcgg ggctctccgg cgccggcgcc tcagtcctca acgagctgtc
      241 gggcatccag gcgtcgctgg cccatcgggg cgtcctgtgg tcggtcgaag acggcaacaa
      301 cggaccgcac ctgttcgcgt tcaacgcggg cggcgaggtg atcggggatt tcaccttgtc
      361 gaaggccgac gtcggcaacc tggactggga agcgctcggc ctggaccggc gcaagggccg
      421 tgaccagctg tacatcggcg acatcggtga caacgcgcac gctcgcgacg gcagcggcca
      481 gaaggtccct gcgctctacc ggatcgccga gccgaagatc cgcgccggcg cctcaggcgt
      541 gacgcgcacg atcacccgga tcaagaagtt caccttccgt tactacacga aggcaggcgt
      601 gctggcgcct cggaacgctg agtcgatgtt cgtcgatccg cggcgccacg acgtgatcgt
      661 gatcaccaag gacctggaga cgatcggtgg tcacgccaac cgtgttcggg cgttcaggat
      721 gcgcgtggga gctctgcacg gcggccggct caaccatgcg aagcaggtgg ccaccctgac
      781 cgcggcgacc gatggccacg gcgtgggggc ggtgtcggcc gacatcgccc gggacggccg
      841 gtggatcgtg gtgaagaact acggccaggg cttcctgtgg catcgcgatc cgcacgggcc
      901 ggcgtggtcg gcgttccgac ggcatccggt cgcgccgtgc cgggtcccgg tggactcggc
      961 cgaggcgatc tcgttctcct acacggacgg cggccgctgg aacgggttct ggtcggtgac
     1021 cgagaccggc tcacccccgc cgccgctccg tcacctggtc ggccccgact gatccatcgc
     1081 tgatccgggc ggttcaagcc ggaccggcac ctgccgaaga cgcgagggag atggacgagt
     1141 ccgagatcgc cgaattgcga gcgcgcgtcg ccgaggccga gcgcgagcgg gacgccgccg
     1201 aagccagggc ccaggccgcc gagacccgtg ctgaggatct cggtgcgctc gatgaccggg
     1261 ttcaggtggc cgagcgccgt gcgctcgacg ctgagcgccg cctgcaggaa ctgtccgagc
     1321 aggtgcaggc gagccggccc ggcacggaca cctccgagga gccgctcgaa cccgtcgggt
     1381 cgaccgagac ggctccgccc gcagcgaacg accttcgggc gcggctggcc cgcacggcgg
     1441 cgcggaagaa gcccggcggc gggccggacc ccacctgagc cgctagaccg cgagcgcgtc
     1501 caccgcgtag gtctcgcgca ggcgttgatc gaccgcttcg gccggcagca cgtgcctcgg
     1561 tgcgagtccg tatctcgacg ccgtctcgag gtgctcgtcg cagatcggca tgagttcgtc
     1621 cttcgggaac cagccgacgt gttcgggcgc cgcagcatac gggcctgcca tccagaagca
     1681 gaactggcag atcagcctgg tgcgctgcgg ataggcgaac cgcgcccggt agccgtactc
     1741 gtgctcgatc accgtgcccc acagcgcgac gcgtcccagc acggccgggc acttggtctt
     1801 gcgcaggatc tccacggcgt gggtgccgtg cagcccgcag gtgcatccgg cgtggggtgc
     1861 ccggtggaag cgcgcaaggg aacacgcggc ttccgcgggc tgccggggct tccagggtcg
     1921 cgaccggcga gcgacgggac gcagcatcag gtcgtcgccg tcgcgcgtgc cggtcagcgc
     1981 ccacgcccgc caggcgagga tgggctctgc gccggccgcg agttccagtc caccagcgcg
     2041 cgacgcggtg gaggtgcggt tcatctcgag cgtcgatccg ccgactcggg ccccgcgggc
     2101 tgaggttcac gttcgggctc gcgctcaggc gtcatgtcgg gcgcgaggtc cggcacgagc
     2161 tcgcccggca ccgggatcgc ggcgggcgtg atctcgatga tgcgtttggg ctggccgata
     2221 tccatgatgc ttcgagggta cgcccctccg ccgagttggg cgagccctac acgggagcca
     2281 accggtagat ggccgagctg tcgctgaagt agatgcgtcc gttcggcgcc cgctccatcg
     2341 agtagatcga accgccgggc acgtcgaaga cggtgaccac gttggcgaag tcgtcgcgtg
     2401 gcccgttcag gacccctctg tgcaggctgc caccgtcaca gcacgctccc cagaacaggt
     2461 ccccttcggc cccggccagc ccgcatccgt cgcagaacac ggcgccggtg atgccgatcg
     2521 tcgagtcgta ggtgaccaac ggcagacgtg ggctcggacc gctgttattc gtgttggggc
     2581 agctctcgct cggaccccag ccgtagttct ccccggcgac gatccggttg acctcgtcgt
     2641 tgcaccctgg gccgttctcc gtttcccaga ggtcgccggt gtccggatcg aacgcgaagc
     2701 cgaaggagtt ccggatgccg taggcgaaca tgcgcgtgcg ctcgcccccg atcgtcgggt
     2761 tgtcggccgg cacgccgcca tcggcccgga gccgcaggat cttcccgcgc aggttcccgg
     2821 tgacgtcctg cgcgttcgtg gagttgtgtc cgtctccgac gatcgcgtac agcctgccgt
     2881 cggggccgaa ctcgatgcgc ccgccgttgt gatacggatc gttcgacgcg ggcgccgaca
     2941 gcagcacctt catgcccacg cccttccagg cgccgtcatg cttgttcgcg cggatgcgaa
     3001 cgatctggtt ccgcaggttc ccgccggcgt tacgggtggc gaagacgtac acgaacggct
     3061 ggtcgggcca cgccgggtgc accgccacgc ccagggctcc acgttccccc gcaccgttga
     3121 cgcccgagac cgtgaagaag cgccgggtgt gcttggtctc gaggttcagg atgcggatct
     3181 gcccgctccc tcgttcggcg tagaggagtt tgccccccgg caggaacgtg aacccggccg
     3241 gccctctcag accgcccttc accttcagga ccttgatcga cgctcccgcg ttggcggccg
     3301 gggccaaggt cacgaccgcg agcgaactcg cgatcagggt caggatcagg agccgcaccg
     3361 tggttcggat cgttcgcatg cgtcgaagtc ctctctgctc ggggagggcg ctcacgcggg
     3421 cgccagtcga tagataccca gggcgttgct gaagtacaga cgatggttcg gcgacgtctc
     3481 catcgagtac accggtccgg cgaagttgac gagggccacc ttctgcaggg cgccctcgaa
     3541 gtcatcgcgc gcgccgttga ggttcgcgcg cgcgaccggc cccacggtct gcttgcaggt
     3601 gccgttcgcg caccccacga acaggtcgcc gtcgtaggcg gcgccgaggc cgcacccgtc
     3661 gcagaacgcg gtgccggtca ccgcgacggt ctcgtcgaag ttccagacgg gcaggagctt
     3721 ggggtcgggg ccgtcctggt tcgtgtcggg acacgcctgg ctcgggcccc aggcgaagtt
     3781 gcccccggcc tggatccggt tgatctcgtc gttgcagtcg ggtccgttct ccgtttccca
     3841 gagggtgccc gaatccgggt cgaacgcgaa cccgatcgag ttgcgatggc cgaacgagta
     3901 caccgtcgag cccgggaacg ggttcgacgg cggcaccgaa ccgttggggt tgagccgcag
     3961 gatcttgccg cgaggttcgt cggtgaggtc ctgcgccgtg gatgggtcct cgccaccgtc
     4021 gccgatcacg acgtagagct tgccgtcggg gccgaacgcg atcctgccgc cgttgtggtt
     4081 ggcgcgcgac ccgatcggtg actgcagcaa cacggtgaag ttgcgcccgt gcccgtgtac
     4141 ggcgcgcagg cggatcagtt gattccggat cggttcgccg ccggggccac gggtgacgta
     4201 gacgtagacg aacggggtcc ggggccagtc ggggtgcagg gccacaccca gcgcgccacg
     4261 ctcgccctcg ctgttgaccc cgccgatccg gaagaacagc cggtccgaac cggtgcgggg
     4321 gttcaggaac cggacctcgc cggtgtcgcg ttccgcgtag acgatgcgtc cgtccggggt
     4381 gaacgtgaag ccgaccgggt tggccagggt cgccacgggg acgacgcgga ccttcggcgg
     4441 cgccgcggtg gcggccggcg atccggcgag ggcgacgatc cccgtcatga gcaccgccgt
     4501 gatcaggacc gtgccggttc gcgtgcttcg gatgtgcttc tccccctctg acgaccgctg
     4561 gtggcctcac gccctgtggg agcatgaggt cgtgcccacg ctgcctccct cgatgttcgg
     4621 ccaaccggcc ctgcgttctg aagacccacg gttcctgacg ggccgcggcc ggtacctcga
     4681 gaacctcccg atcgagggcg tgctccgagc ggtgttcgtt cgctccatga tgccgcacgc
     4741 gcgcgtgatc gcggtggacg tcgacgccgc acgggccatg cccggggtgg tcgcggtcta
     4801 caccgcagac gatctggcca tccgcgcgat gccccccagc ggcaacgtgg agggggcctc
     4861 gggcacgctc gaagggctgt tcggccgcga ggtgatggcc cgcgaggtgg tgcgcttcgt
     4921 gggagaaccg ctcgcgctgg tgg
//
LOCUS       DATDBZ010000265         2388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_60334,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000265 DATDBZ010000000
VERSION     DATDBZ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60334"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..253
                     /locus_tag="VIX62_06495"
     CDS             125..253
                     /locus_tag="VIX62_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859914.1"
                     /translation="MSDPSTMTCAYQTVFPEPSTTIQASLAGSMPSWLHIPGGSPR"
     gene            complement(387..1169)
                     /locus_tag="VIX62_06500"
     CDS             complement(387..1169)
                     /locus_tag="VIX62_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859915.1"
                     /translation="MEPRSADRLRRISVPLVIGVLAVVLVLSLWGSWSRGPVETTAVF
                     SSAGGSTPPAATPEPVPLPAWWPERLTLYTDSVGLGAVTALRDVMPTWRVKVLGRPAL
                     MLDIAAEELADDDAQVDEVVVVALGYNSLWEHNREDFDYYSRTFDQDAARLVRTLHAA
                     GARKIVWVLLRDAPRSVIPADSRDQNRSFSWYFPYVNERLRKLDRERSDVVLADWSSI
                     GDAEDITYDAIHLDPDGALLYARMVKKAIMHEPYEPFGSQEP"
     gene            1267..>2388
                     /locus_tag="VIX62_06505"
     CDS             1267..>2388
                     /locus_tag="VIX62_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY5859916.1"
                     /translation="MKILVVGSGGVGAAFAPIAARRDFYEHIVFADLDSSRSERVVDR
                     YDGGTGRFSATTVDASDAANVAECVRANGITHVLNAADPRFVMPIFTGAFEAGVHYLD
                     MAMSLSERDPDQPYERVGKKLGDDQFAVSGDWEARGLLALCGIGIEPGAADVFAKYAA
                     TSLFSQIDEVGVRDGANLEIAGYGFAPTFSIWTTIEECLNPPVIWEKARSDVDGGWFT
                     TAPFSEPEVFTFPDGIGDVECVNVEHEEVLLVPRWVKTDRVTFKYALGDDFIDKLKTI
                     HALGMDRTDRVTVKGVEVAPRDVLAAITPDPISLGDKFRGRAVVGTWVIGTKGGEPRE
                     TYAYQMCDAEEIWREFGLQIVGWQTGFNPTVAMELLATGA"
BASE COUNT          408 a          785 c          809 g          386 t
ORIGIN      
        1 aagtcagcac ttcgttcagc ttcgactagt cccaaagcag gtgcgcgcct cgacctggtc
       61 caagaggaag gacccgaggg cccgcctccg atgcagggga tggacgaccc cgagggattc
      121 gacgatgtcc gatccgtcca ccatgacgtg cgcgtatcag acggtcttcc ccgagccgtc
      181 gaccacgatc caggcctccc tggcgggatc gatgccgtcg tggctccaca tccctggagg
      241 aagtccgcgt tgagtacaac ctcgcccgcg tcgtcgaggt cgtcggctcc gaagatgtcg
      301 gcgaccgcgg gaagttcacc ctcgatcggt gctcgggatg tgtatccggc cgccggttcc
      361 atccctatca gcgaacgctc cgcccgctag ggctcctgcg aaccgaacgg ttcgtacggt
      421 tcgtgcatga tcgccttctt caccatgcgt gcgtagagca gcgctccgtc cggatccagg
      481 tggatcgcgt cgtaggtgat gtcctccgcg tcgccgatcg aggaccagtc ggccagcacc
      541 acgtcggagc gctcgcggtc gagcttgcga aggcgctcgt tcacgtacgg gaagtaccag
      601 gaaaaggacc ggttctgatc gcgcgaatcg gcggggatga ccgatcgggg cgcgtcgcgc
      661 aacaggaccc agacgatctt gcgagcgcca gcggcgtgca gcgtgcggac cagccgagcg
      721 gcgtcctggt cgaacgtcct cgagtagtag tcgaagtctt cccggttgtg ctcccacaac
      781 gagttgtacc cgagtgccac gaccaccact tcatcgacct gtgcatcgtc gtcggcgagc
      841 tcttccgctg cgatgtcgag catgagggcg ggcctgccca gcaccttgac gcgccaggtc
      901 ggcatcacat cgcggagggc ggtcacggcg cccagcccca cggaatcggt gtacagcgtg
      961 agccgttctg gccaccaggc gggcaggggc acgggttctg gcgtcgcggc cggcggcgtg
     1021 gagcctcccg ccgatgagaa gaccgccgtg gtttcgaccg ggccacgcga ccaggatccc
     1081 cacagcgaca gcacgagcac cacggccagc accccgatca ccagtgggac cgagatgcgc
     1141 ctgagccggt cggcggagcg gggttccatg ggacggttcc ttcgaggcgt tgggtcggag
     1201 agcgcaccgg gccatgcacc cggttcgcag gtgggcaatg gtacccgccc gctagactcc
     1261 cgcgccatga agatcctggt ggtgggctcc ggtggggtgg gggcggcgtt cgcaccgatc
     1321 gcagccaggc gagacttcta cgaacacatc gtgttcgccg atctcgactc ctcgcgatcg
     1381 gagcgggtgg tggatcgcta cgacggcggc accggacgct tcagcgctac cacggtcgat
     1441 gcgtccgacg ccgccaacgt cgccgagtgc gttcgggcca acggcatcac gcacgtgctc
     1501 aacgccgccg acccccggtt cgtcatgccg atcttcaccg gggctttcga ggctggggtg
     1561 cactatctgg acatggcgat gtcgctgtcc gaacgagacc ccgatcagcc atacgaacgc
     1621 gtcggcaaga agctggggga cgaccagttc gcggtctccg gcgactggga ggcgaggggc
     1681 ctgctggcac tgtgcggcat cggcatcgaa ccgggtgcgg ccgacgtatt cgcgaagtac
     1741 gccgcgacgt cgctgttcag tcagatcgac gaggtgggtg tgcgcgacgg cgccaacctg
     1801 gagatcgccg ggtacgggtt cgcgcccacc ttcagcatct ggaccacgat cgaggagtgc
     1861 ctcaatccgc cggtgatctg ggagaaggct cgctcagacg tggacggcgg ttggttcacc
     1921 accgccccgt tcagcgaacc cgaggtgttc accttccccg acggtatcgg ggacgtggaa
     1981 tgcgtcaacg tcgagcatga ggaggtgctc ctcgtcccgc gctgggtcaa gacagaccgc
     2041 gtgacattca agtacgcgct cggggacgac ttcatcgaca agctcaagac gatccacgcc
     2101 ctgggcatgg accgcaccga cagggtcacc gtcaagggcg tcgaggtcgc tccgcgcgac
     2161 gtcctcgccg cgatcacccc cgacccgatc agcctcggcg acaagttccg agggcgagcc
     2221 gtggtgggga cgtgggtcat cggcacgaag ggcggcgagc cgcgcgagac ctacgcgtac
     2281 cagatgtgcg acgccgagga gatctggcgc gagttcggtc tccagatcgt cggctggcag
     2341 accggcttca acccgaccgt ggccatggag ctgctcgcca cgggcgcc
//
LOCUS       DATDBZ010000266         5579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_906732, whole genome shotgun sequence.
ACCESSION   DATDBZ010000266 DATDBZ010000000
VERSION     DATDBZ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906732"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VIX62_06510"
     CDS             <1..627
                     /locus_tag="VIX62_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY5859917.1"
                     /translation="VISYNRFISQKNLIKDAWANIDTELKRRYDLIPNLVETVKGYAA
                     HEKEVFEEVARTRSMAAGATGSPASQAAAEGPLVAALGKLFAVAENYPDLKANQNFLE
                     LQRELTNTEDRLQTARRFYNANVREYNQRVKQFPSVLVASAFHFEEAEFFEVDEAIRE
                     AGAPQVDFGTAGGSGATPPAAGGAATLPPPADDVPEPPGQPTPPNPTG"
     gene            complement(624..1964)
                     /locus_tag="VIX62_06515"
     CDS             complement(624..1964)
                     /locus_tag="VIX62_06515"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859918.1"
                     /translation="MGTPKPEADEHQAPPGGVRASVSLLRRNRDFRRLFLASVISLGG
                     DWFLFVAITGLILETTGRAVDVGLAIMAQELAFFLASPAAGVLADRVDRRRLMIVCDL
                     VRAVICSAFLLVGPETVWLAYPLLAALSVFSAPFEPASSAAVPNVVDAEDLPSANAIA
                     GSLWGTMLAVGAALGGVISTVFGRDPAFVVDALSFLLSALFLAGIRRSFSEARAADHE
                     HPKIVEATKEVLHYARTDRRVSALITVKGGFGLAAGVLALIPVFGADVFGKGDIGVGI
                     LMAARGVGALVGPFFGHRLSGPQHRRLFSAIGLALTVFGLSYMGLGLAPALWVAAVAI
                     FVAHLGGGTQWVLSTYGLQVLVPDHIRGRIFAVDFALITLSLGISSLAASWIADAAGP
                     RQAAFALGGVAVLWAGVWWILTRKVRKGPALEGAEPDEDAYEPPEPEAVALGDR"
     gene            2012..2653
                     /locus_tag="VIX62_06520"
     CDS             2012..2653
                     /locus_tag="VIX62_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5859919.1"
                     /translation="MLERITWFRQSALRWVDDERTIYIDPWGTPEDAPPADLILITHA
                     HLDHFQPEEIERLRGPGTKLVAPRDVADELTGDVTAVAPDQKHEVAGVPFTTVPAYNT
                     REEALDFHPRANDWVGYVLELGGATYYHAGDTDHAPELETIDTDVAFLPVGGYYTMNV
                     EEAAGLALAMTPRLAVPMHYGFVVGSATDGDRFRDAASPVAVDLMDPTNPFEC"
     gene            2715..3620
                     /locus_tag="VIX62_06525"
     CDS             2715..3620
                     /locus_tag="VIX62_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEY5859920.1"
                     /translation="MALGYEGDLYILAFDHRGSFQKKFFGIDGAAGPEDTLKIIDAKA
                     VIFEGMRRALADGVSNEAAGVLVDEQFGAEVARAAIRDGLKLAMPAEKSGQHEFDFEY
                     GEEFSAHIEAFDPAFTKVLVRYNPEGDGAMNARQAVRLKQLSDWLHHTGRRFLFELLV
                     PAEPHQLETVANDVGRFDREVRPHLMRVAIAELQATGVEPDIWKIEGIDDPEQCREVA
                     EQCRIGGRDGVACIVLGRGADDAAVDAWLRAARGVPGYQGFAIGRSIWWNPLKFFVDG
                     ALPREEAVSQIANNYRRFVAVWEQG"
     gene            3636..3839
                     /locus_tag="VIX62_06530"
     CDS             3636..3839
                     /locus_tag="VIX62_06530"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEY5859921.1"
                     /translation="MAVIKTIDLVGVSGESWRDAAQQALAEAAKTIRGIESMDILETS
                     AKVADGQISEYHTHVRIRFRIER"
     gene            complement(3885..4655)
                     /locus_tag="VIX62_06535"
     CDS             complement(3885..4655)
                     /locus_tag="VIX62_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859922.1"
                     /translation="MKRIWDEIGPPVFGIVALIVVWAIVAALTDTRGVPSPAEVWSAF
                     LTGMRDGTIPEATVKTLLRLLFAFACSIAIGVAIGVGMALNEFTRRSVRPLIVALQIT
                     PFVAWLPLAVIWFGASERAVVFVTIVGAFPSVTLATMSAIRQVPPILTRAGRTMGADG
                     WGLYRDVILPAAMPGFVAGLQQAWGFAWKALMAAELIITAVGASGLGHLLAEEQSNVP
                     ELLAVLGVIVVIGVAVDYLLFHRLDRRIRKRRGLLVEG"
     gene            complement(4652..5482)
                     /locus_tag="VIX62_06540"
     CDS             complement(4652..5482)
                     /locus_tag="VIX62_06540"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5859923.1"
                     /translation="MSDAATNIVDLASVRDAGPPKLEVRDLVKTRRSTTGTELTAVDH
                     VSFQVGEGEFVCLLGPSGSGKTSILNILAGLERPTSGSALLDGTPITAPGPDRAVLFQ
                     EPALFPWLSVAGNVELALKLIDVPADQRRERADAWLTNVGLERFADVQPHELSAGMRQ
                     RAALARALACEPDVLLGDEPFGALDAQARELLQDEVQRVWVERDGRTTFVFVTHNVRE
                     AALLADRVLVLSAAPGKLIEEVRINAPRPRQLDDVLVARVVSEVHELLMREVDKGANR
                     "
     gene            complement(5493..5576)
                     /locus_tag="VIX62_06545"
     CDS             complement(5493..5576)
                     /locus_tag="VIX62_06545"
                     /inference="COORDINATES: protein motif:HMM:NF021566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HEY5859924.1"
                     /translation="MQYGTVVLVIQDSKVVQIEMAEKFRLR"
BASE COUNT          982 a         1962 c         1881 g          754 t
ORIGIN      
        1 gtgatctcct acaaccgctt catctcgcag aagaacctga tcaaggacgc ctgggccaac
       61 atcgacaccg agctcaagcg ccggtacgac ctgatcccga acctggtcga gaccgtgaag
      121 ggctacgccg cccacgagaa ggaggtcttc gaggaagtcg cgcgcacgcg ttcgatggcc
      181 gcgggcgcca ccgggtcgcc ggcctcccaa gccgccgccg aaggcccgtt ggtcgccgcc
      241 ctcggcaagc tgttcgccgt cgccgagaac taccccgacc tcaaggcgaa tcagaacttc
      301 ctggagctgc agcgagagct caccaacacc gaggatcgcc tgcagacggc acggcgcttc
      361 tacaacgcca acgtccgtga gtacaaccag cgcgtgaagc agttcccgtc cgtgctggtc
      421 gccagcgcct tccacttcga agaagcggag ttcttcgagg tcgacgaggc gatccgcgag
      481 gccggcgctc cccaggtgga cttcggcacc gctggtggct cgggcgccac gcctcccgcc
      541 gccggaggcg ccgccaccct gccaccgccg gccgacgacg tgccggaacc ccccgggcag
      601 cccaccccgc cgaacccgac cggctagcgg tcgcccaggg ccacggcttc gggctcggga
      661 ggttcgtagg cgtcctcgtc cggctcggcg ccctcgagtg ccggtccctt gcggaccttg
      721 cgggtgagga tccaccagac gccggcccac agcacggcga ccccgcccaa cgcgaacgcg
      781 gcctgccgcg gcccggcggc gtcggcgatc caggaggcgg cgaggctcga gatccccaac
      841 gacagcgtga tcagcgcgaa atcgacggcg aagatgcggc cccggatgtg atccggcacc
      901 agcacctgga gcccgtaggt cgacagcacc cattgtgttc cgccaccgag gtgggccacg
      961 aagatcgcca cggccgccac ccacaacgcg ggagccagcc ccaaccccat gtacgacagc
     1021 ccgaacacgg tcagcgcgag gccgatcgcg ctgaacaatc gtcgatgctg cggcccgctg
     1081 agccggtgac cgaagaacgg acccaccaag gcgcccacgc cgcgtgccgc catcaggatg
     1141 cccacaccga tgtcaccttt gccgaacacg tcggccccga acaccgggat caatgccaag
     1201 acccccgccg cgaggccgaa cccgcccttg accgtgatga gcgccgagac ccgacgatcg
     1261 gtgcgggcgt aatgcaagac ctctttggtg gcctcgacga tcttgggatg ctcgtggtcg
     1321 gcggcccgtg cttcgctgaa cgagcgccgg atcccggcca ggaacagcgc ggacagcagg
     1381 aacgacagcg cgtccaccac gaaggcggga tcgcgcccga acaccgtgga gatcacaccg
     1441 ccgagtgccg cccccacggc cagcatcgtt ccccacaggg agcccgcgat cgcgttcgcc
     1501 gaagggaggt cctcggcatc cacgacgttg ggcaccgccg ccgaggaggc cggttcgaag
     1561 ggcgcgctga acaccgacaa cgccgcgagc aaggggtagg cgagccacac cgtttcgggt
     1621 cccaccagca ggaacgccga gcagatcacc gcgcgaacca ggtcgcacac gatcatcagc
     1681 cggcgccggt ccacgcggtc ggccagcaca ccggccgccg gcgaggccag gaagaaggcc
     1741 agctcctggg ccatgatcgc caggcccacg tccaccgcgc ggccggtggt ctccaggatc
     1801 aggcccgtga tcgccacgaa caggaaccag tcgccaccca gcgagatcac cgaggccagg
     1861 aacaggcgcc ggaaatcacg gttgcgccgc aacagggaaa cgctggctcg gacccctccg
     1921 ggcggggcct ggtgttcgtc ggcttcgggt ttgggcgttc ccatggctgg gcagtcagac
     1981 tatctggggc ccgtctcgtg aggaggtcgc cgtgctcgaa cggatcacct ggttccgaca
     2041 gtcagcgctc cgatgggtcg acgacgaacg cacgatctac atcgatccgt ggggcacgcc
     2101 cgaggacgcc ccacccgccg acctgatcct gatcacgcac gcccacctcg atcatttcca
     2161 gcccgaggag atcgaacggc tcaggggacc cggaacgaaa ctggtggcgc cccgcgatgt
     2221 cgccgacgaa ctcaccggag acgtcacggc ggtggcgcca gaccagaagc atgaggtcgc
     2281 cggcgttccg ttcaccaccg tgcccgcgta caacacgcgt gaagaggcgc tggacttcca
     2341 tccccgggcc aacgactggg tggggtacgt gctcgagctc ggtggcgcca cctactacca
     2401 cgcgggtgat actgaccatg cgcccgagct cgagacgatc gacacggatg tcgcgttcct
     2461 tcccgtgggc ggctactaca cgatgaacgt ggaggaagcc gcgggtcttg cgctggcgat
     2521 gaccccacgg ctcgccgtgc cgatgcacta cggcttcgtg gtgggttcgg ccacggacgg
     2581 cgaccggttc cgagacgccg cctcccccgt ggcggtcgac ctgatggacc ccaccaaccc
     2641 gttcgaatgc tgaggcgaac gtcggacccg gagcgatagg ctcggggaac caaccgcgaa
     2701 ccgaggggag acgaatggcg ctcggctacg aaggcgacct gtacatcctg gcgttcgatc
     2761 accgaggatc gttccagaag aagttcttcg gcatcgatgg tgcggccggg cccgaggaca
     2821 cgttgaagat catcgacgcg aaggccgtga tcttcgaggg gatgcgacgc gcgttggccg
     2881 acggcgtcag caacgaggcg gcaggggtgc tcgtcgacga gcagttcggc gccgaggtgg
     2941 ctcgggccgc gatccgcgac ggactcaagc tcgcgatgcc cgccgagaag tccggccaac
     3001 acgagttcga cttcgagtac ggcgaggagt tctccgcgca catcgaggcg ttcgacccgg
     3061 ccttcacgaa ggtcctggtg cgctacaacc ctgaaggcga cggcgcgatg aacgcacgcc
     3121 aagccgttcg gctgaagcag ttgtcggact ggctgcatca caccgggcgc cgcttcctgt
     3181 tcgaactgct cgtcccggcc gaaccccatc agctggagac ggtcgccaac gacgtcggcc
     3241 ggttcgaccg ggaggtgcgt ccccacctga tgcgggtggc gatcgccgag ctgcaggcca
     3301 ccggcgtgga gccggacatc tggaagatcg aggggatcga cgatcccgaa cagtgtcgcg
     3361 aggtggccga gcagtgccgg atcggcgggc gcgacggcgt ggcgtgcatc gtcctgggac
     3421 gtggtgccga tgacgcggcg gtcgatgcct ggctgcgtgc cgcgcgtggc gtccccggct
     3481 atcaggggtt cgcgatcggc cggtcgatct ggtggaaccc gttgaagttc ttcgtcgacg
     3541 gcgcgctccc gcgcgaagag gccgtctcgc agatcgcgaa caactaccgg cggttcgtcg
     3601 ccgtgtggga acaaggctag gggaaggaga tcgccatggc ggtcatcaag acgatcgatc
     3661 tggtcggggt gtcgggcgag tcctggcgcg acgccgcgca gcaggcgctg gccgaagcgg
     3721 ccaagacgat ccgagggatc gagagcatgg acatcctgga gacctcggcg aaggtcgcgg
     3781 acggacagat cagcgagtac cacacgcacg tgcggatccg gttccgcatc gagcgctgat
     3841 cgctccgagc cacgccggct gagcggccct ttcgggcggc cggctcagcc ttcgaccagc
     3901 agcccgcgcc gcttgcggat gcgccggtcg agccggtgga agagcaggta gtccaccgcg
     3961 acgccgatca ccacgatcac gccgagcacg gccaagagct cggggacgtt gctctgttcc
     4021 tcggcgagca gatgccccag ccccgaggcg cccaccgcgg tgatgatgag ttcggcagcc
     4081 atcagcgcct tccacgcgaa accccacgcc tgctgcagcc cagcgacgaa gccgggcatg
     4141 gccgccggca ggatcacgtc ccggtacagg ccccagccgt cggcacccat cgtccggccc
     4201 gcccgggtga ggatcggcgg cacctgccgg atcgccgaca tggtcgccag cgtcaccgac
     4261 gggaaggccc ccacgatcgt gacgaacacc acggcgcgtt cgctggcgcc gaaccagatc
     4321 accgcgaggg gcagccacgc cacgaacggt gtgatctgga gcgccacgat caacggtcgg
     4381 accgatcgcc gcgtgaactc gttgagggcc atgcccacgc cgatcgccac gccgatcgcg
     4441 atcgagcacg cgaacgcgaa cagcagccgg agcagcgtct tgaccgtggc ctcggggatc
     4501 gtgccgtcgc gcatgcccgt gaggaaggcg ctccacacct cggcggggga gggcacgccg
     4561 cgcgtgtcgg tgagggccgc cacgatcgcc cacaccacga tcagcgccac gatgccgaac
     4621 acgggcggac cgatctcgtc ccagatccgc ttcaccggtt cgcccccttg tcgacctcgc
     4681 gcatcaggag ctcgtgcacc tcggagacga cgcgggccac caggacgtca tcgagctggc
     4741 ggggccgcgg cgcgttgatg cgcacctcct cgatcagctt gccgggggcg gccgacagga
     4801 cgagcacccg gtcggcgagg agcgccgctt cccgcacgtt gtgggtcacg aacacgaagg
     4861 tcgtgcgccc gtcgcgttcc acccacaccc gctggacctc gtcctggagg agttcgcgcg
     4921 cctgcgcgtc gagcgcgccg aagggttcgt cgcccagcag cacatcgggt tcgcacgcga
     4981 gggcgcgagc gagcgccgcc cgctgccgca tgccggccga cagctcgtgc ggctgcacgt
     5041 cggcgaatcg ctcgaggccg acgttggtga gccaggcgtc ggcgcgttcc cgtcgctggt
     5101 cggcgggcac gtcgatcaac ttcagcgcga gctcgacgtt gccggccacc gacagccacg
     5161 ggaacaacgc cggctcttgg aacaacaccg cgcggtcggg gccgggcgcg gtgatcggcg
     5221 tcccgtcgag caacgccgaa cccgacgtgg ggcgttccag cccggccagg atgttgagga
     5281 tcgaggtctt gccgctgccc gacggcccca gcaggcacac gaactcgccc tcgcccacct
     5341 ggaacgacac gtggtcgacc gcggtgagct cggtgcccgt ggtggacctg cgcgtcttca
     5401 cgaggtcgcg gacctccagc ttgggaggtc cggcatcacg aaccgatgcc aggtcgacga
     5461 tgttggtggc ggcgtcgctc atcggggcgt cctcatcgca gccggaactt ctcggccatc
     5521 tcgatctgca cgaccttgct gtcctggatc accaggacca cggtgccgta ctggatctt
//
LOCUS       DATDBZ010000267         4644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2938741, whole genome shotgun sequence.
ACCESSION   DATDBZ010000267 DATDBZ010000000
VERSION     DATDBZ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2938741"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VIX62_06550"
     CDS             complement(<1..399)
                     /locus_tag="VIX62_06550"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY5859925.1"
                     /translation="MAGTDRLRSRDVTEGPERAPARAMLRAVGFSEEDFGKPQIAVAS
                     SWNEVTPCNYHLDKLAALAKEGVRQGEAVPVEFTTIAVSDGIAMGHEGMKASLMSRDL
                     IADSVELVMHAERFDGLVGIAGCDKSEPGML"
     gene            610..2043
                     /locus_tag="VIX62_06555"
     CDS             610..2043
                     /locus_tag="VIX62_06555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5859926.1"
                     /translation="MADIATIPIPPDAGRSWWLTEALARPEFAGEPSPALTEDTTADV
                     VILGGGYTGMWTAFHLKQLDPGTDVVILEQDICGGGPSGRNGGFVNSYWGDLPRLVSE
                     FGDKAAISLCEAGEESVQAISAFCDEHDVDAWFANDGDLNAAASDAQVGSWGDLIITA
                     DRLGLAEDFRVMGPQEVRAMVDSPTFKGGVFTKYGASVQPARLARGLRRVLMDQGVRI
                     YEQTPVTRFGSGSPAIAETPRGTVRVGAGVIALNAWASHWKRFRRAITVRGSYIVMTA
                     PAPEKLAEIGWTDGMGLSDYRAALHYLRTTPDGRIAFGIGGMQPNLARHIGPRFAYDE
                     QALQVAARDLHRMFPTFADVPIDAGWGGPIDVAGSHLPFFGTLEHGTVHYGMGYTGNG
                     VGPAHLGGRILANRSLGRYDDVLDLPIVDARPMKFPPEPIRSPGALIANHAIRRKDEF
                     EDRGEDPNPLVDFVAKLPRRLGYNLGP"
     gene            complement(2040..3158)
                     /locus_tag="VIX62_06560"
     CDS             complement(2040..3158)
                     /locus_tag="VIX62_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY5859927.1"
                     /translation="MEPIDLTTLSSDVRRGPLFERQRAMGATFYEDYGRLWAASFGDL
                     DREYAAIRTGAMAWDISPLSKFHMVGPQVREALERLTSRPISGEEPGQVRYLVILDEA
                     GHIVDEGTRYLIGPDEAWYVGNEDRPALVEHIAAALEGFDVTTTNRTDEVAALAIQGP
                     EAFDVLAPLIDVDLASLDYYRCRPDVAVAGVPAMISRTGFSGELGYELFVPDGVDRVW
                     DGLLEAGATPVGLDAVEMARVEVGFLVADEDYVSFETDPYEVGLGEFIDLTGHEFVGR
                     EAALAASQDEHRELVTLVFDEPEVLPTPGQVTLDRRVVGEVRSVERSPRFGMLGLAVL
                     DADLAVDGAFVEVDGIAAVVRPRPIDDGDRARRTRPGG"
     gene            complement(3269..>4644)
                     /locus_tag="VIX62_06565"
     CDS             complement(3269..>4644)
                     /locus_tag="VIX62_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5859928.1"
                     /translation="LAAAVRDGRTTSSALVAESLHRIEAGDGPVNAVVLRRDEDALAD
                     AAALDARVAAGQDPGPLAGLPLLVKDIEDCAGLPTTFGSLLRRDAAPAERDCRAVARL
                     RAAGAVVVGKTNTPEFAFEGFTSNRLFGDTHNPWALDWSPGGSSGGSGAALAMGLAPL
                     ATATDGGGSIRIPAAFCGLVGLKPTKGLIGDDPIPSWIDLSSKGPLTVSVADAALLLD
                     VLRGPTDGDPTAVPAWSPREGVWPSRLMVTPRLVEGGPLPDAVAALFDEAVASLEMVT
                     GLTAEPIASPFPPQLDEDWFIAVGAEELTWIGRDVVIEHADELTAYVRGGLEYAATFT
                     VDDYLAARRRRFEFVKVIDQLLGPDAIVVSPTMCVEGFYADGRAPGEDDAGSTQAPAY
                     NTQAANITGHPALSLPAGRSPNGVPFGIQLTGPRFADDLLLAVGAAWEAAYPWPLSAP
                     GFEPFGR"
BASE COUNT          727 a         1678 c         1585 g          654 t
ORIGIN      
        1 caacatgccg ggctcggatt tgtcgcagcc cgcgatgccc accaacccgt cgaaccgctc
       61 cgcgtgcatc accagctcga ccgaatcggc gatcaggtcg cggctcatca ggctggcctt
      121 catgccttca tggcccatcg cgatgccgtc ggagaccgcg atcgtggtga actcgacggg
      181 aaccgcctcg ccctgacgca cgccttcttt cgcgagcgcg gcgagcttgt ccaggtggta
      241 gttgcagggg gtgacctcgt tccagctgga ggccaccgcg atctgcggtt tgccgaaatc
      301 ctcttccgag aacccgacgg cgcggagcat ggcgcgggca ggggcgcgct cgggaccttc
      361 ggtgacgtct cggctgcgga gacgatcggt gcctgccatc gtggagcctc ctcggcggat
      421 gggatgggga cctcacggta gcaacgccag cccggggctg acctgggcgg accgataccg
      481 gaaccgatat ccacgacgca gatggcatcg gggactccac atcgataccg gttccggcat
      541 gagaagggac cgctcgaccg ttccgacgaa ccacatcgct ctttcggtat cggtacccta
      601 gggtcctgca tggctgacat cgcgacgatc ccgatcccgc ccgacgccgg ccggagctgg
      661 tggctcaccg aagcgctcgc gcgaccagag ttcgccggcg aaccttcgcc cgcgctcacc
      721 gaggacacca cggccgacgt cgtgatcctg ggcggcggct acaccgggat gtggaccgcg
      781 ttccacctga agcagctcga ccccggcacc gatgtggtga tcctggagca ggacatctgc
      841 ggcggcgggc ccagcggccg caacggcggg ttcgtgaaca gctactgggg cgacctgccg
      901 cgactggtct cggagttcgg cgacaaagcc gcgatctcgc tctgtgaagc gggcgaggag
      961 agcgtgcagg cgatcagtgc cttctgcgac gagcacgacg tcgacgcatg gttcgcgaac
     1021 gacggcgacc tcaacgcggc ggcctccgac gcgcaggtgg ggtcttgggg cgatctcatc
     1081 atcaccgccg accgcctggg cctggccgag gacttcaggg tcatggggcc ccaggaggtc
     1141 cgggccatgg tcgattcgcc cacgttcaag ggcggcgtct tcacgaagta cggcgccagc
     1201 gtgcagccgg cgcggctggc gcgtggcctg cgacgcgtgc tgatggacca gggcgtgcgc
     1261 atctacgagc agaccccggt gacccggttc ggttcggggt cgcccgcgat cgccgagacc
     1321 ccccgcggca ccgttcgcgt gggtgccggc gtgatcgcgc tcaacgcgtg ggccagccat
     1381 tggaagcggt tccgccgcgc gatcacggtt cgtggcagct acatcgtgat gacggcaccg
     1441 gcgcccgaga agctcgccga gatcggctgg accgacggca tgggattgtc cgactaccgc
     1501 gccgcgctgc actacctgcg caccacgccc gacggccgta tcgcgttcgg catcggcggc
     1561 atgcagccga acttggcccg ccacatcggc ccgcggttcg cctacgacga gcaggcgcta
     1621 caggtggcgg cccgggacct gcaccggatg ttccccacgt tcgctgacgt gcccatcgac
     1681 gcggggtggg gcggtccgat cgacgtcgcc ggctcgcacc tgccgttctt cggcacgctc
     1741 gagcacggca ccgtgcacta cggcatgggc tacacgggca acggcgtggg ccccgcccat
     1801 ctgggcggtc gcatcctggc gaaccgctcg ctcggccgat acgacgacgt gctcgacctg
     1861 ccgatcgtcg atgcgcgccc gatgaagttc ccgcccgagc cgatccgctc ccccggtgcg
     1921 ctcatcgcga accacgcgat ccgccgcaag gacgagttcg aggaccgcgg cgaagacccg
     1981 aacccgctcg tggatttcgt ggcgaagctc ccgcgccgcc tgggctacaa cctcggccct
     2041 tagccgcccg gccgcgtgcg ccgcgcccgg tctccgtcgt cgatgggccg aggacgcacc
     2101 accgccgcga tgccgtcgac ctccacgaac gcaccgtcca cggccaggtc ggcgtcgagg
     2161 accgcaaggc ccagcatccc gaaccggggc gaccgttcca cgctgcggac ctctcccacc
     2221 acgcggcggt cgagggtgac ctggccgggg gtcggcagca cctcgggttc gtcgaacacc
     2281 agcgtgacga gctcccggtg ctcgtcctgc gaggcggcga gggcggcctc cctgccgacg
     2341 aactcgtgcc cggtcagatc gatgaactcg cccagcccga cctcgtaggg atcggtctcg
     2401 aacgagacgt agtcctcgtc ggccaccagg aacccgacct cgacgcgtgc catctccacg
     2461 gcgtcgagcc cgaccggcgt cgcccccgcc tccaggagcc cgtcccacac ccgatccacg
     2521 ccgtcgggga cgaacagctc gtatccgagt tcgccggaga agccggtgcg cgagatcatc
     2581 gccggcaccc ccgccaccgc cacgtcgggc cggcaccggt agtagtcgag cgacgcgagg
     2641 tcgacgtcga tcagtggggc cagcacatcg aacgcctccg ggccctggat cgcgagggcg
     2701 gccacctcat ccgtgcgatt ggtcgtggtg acgtcgaacc cttcgagcgc cgccgcgatg
     2761 tgttcgacca gcgccggtcg atcttcgttg cccacgtacc aggcctcgtc ggggccgatc
     2821 aggtagcgcg tcccctcgtc cacgatgtgc ccggcctcgt cgaggatcac cagataccgg
     2881 acctggcccg gctcctcgcc ggagatcggc cgggacgtga gccgctcgag cgcttcgcgg
     2941 acctgcggtc ccaccatgtg gaacttcgac agcggcgaga tgtcccaggc catcgccccg
     3001 gtacggatcg cggcgtactc gcgatcgagg tcaccgaagg aggccgccca cagccggccg
     3061 tagtcctcgt agaaggtggc gcccatcgcg cgctgccgct cgaacagcgg cccacgccgc
     3121 acgtcggagg acaaggtcgt cagatcgatc ggttccacgc tgggctgctc cagggttggc
     3181 cggtggacgc ccgaagccta gcggtcgacc accgcggaaa ccagcatcga ccgtcgggtg
     3241 cggccgcaaa cctcttccga cacgccgctc agcggccgaa cggttcgaag cccggggccg
     3301 acagcggcca ggggtacgcc gcttcccagg cggcgcccac cgccagcagc aggtcgtcgg
     3361 cgaaccgggg cccggtgagt tggatcccga agggcacgcc gttgggcgaa cgtccggccg
     3421 gcaggctcag ggcaggatgc ccggtgatgt tcgcggcctg cgtgttgtag gcgggggctt
     3481 gggtgctccc cgcgtcgtcc tcgcccgggg cccggccgtc cgcgtagaag ccttcgacac
     3541 acatcgtggg cgacaccacg atcgcatcgg gccccaggag ttggtcgatc accttgacga
     3601 actcgaacct gcggcgacgg gccgccagat agtcatcgac ggtgaaggtc gccgcatact
     3661 ccaacccgcc gcggacgtag gccgtgagct cgtccgcgtg ttcgatcacc acgtcacgac
     3721 cgatccaggt gagctcctcg gcgcccaccg cgatgaacca gtcctcgtcg agctgcgggg
     3781 ggaacggcga ggcgatgggt tcggccgtga gtccggtcac catctccaac gacgccacgg
     3841 cctcatcgaa cagggcagcc accgcatcgg gcaacggtcc gccctcgacc aggcgagggg
     3901 tcaccatcag ccgggagggc cacacgcctt cgcgcggcga ccacgcgggg accgcggtgg
     3961 ggtcgccgtc ggtcggccca cggagcacgt ccagcagcag ggctgcgtcg gcgaccgaga
     4021 ccgtcaacgg acccttcgac gacagatcga tccacgacgg gatgggatcg tcgccgatca
     4081 ggcctttggt gggcttcagg ccgaccaacc cacagaaggc cgccgggatc cggatcgacc
     4141 ccccaccatc ggtggcggtg gcgagcggag ccaggcccat ggcgagcgcg gcaccgctcc
     4201 ccccactcga gccgccgggc gaccaatcca acgcccaggg attgtgcgtg tcgccgaaca
     4261 gccggttcga ggtgaacccc tcgaacgcga actcgggggt attggtcttg ccgaccacca
     4321 ccgctccggc ggcacggagc ctcgccacgg cccggcagtc gcgttcggcc ggcgccgcat
     4381 ccctgcgcag cagcgatccg aaggtcgtgg gcagaccggc gcagtcttcg atgtccttga
     4441 ccagcagcgg caggccggcc agcggaccgg gatcctggcc cgcggcgacg cgcgcgtcga
     4501 gcgcagcggc gtcggcgagc gcgtcctcgt cgcggcgcag gaccaccgcg ttcaccggac
     4561 cgtcgccggc ctcgatgcgg tgcagggact cggccaccaa cgcggacgac gtggtgcggc
     4621 cgtccctgac ggcggcggca agct
//
LOCUS       DATDBZ010000268         4599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1722392, whole genome shotgun sequence.
ACCESSION   DATDBZ010000268 DATDBZ010000000
VERSION     DATDBZ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722392"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VIX62_06570"
     CDS             complement(<1..332)
                     /locus_tag="VIX62_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5859929.1"
                     /translation="MADEPAVEGLQPATAIAITAVRRGLDLALSRVGAGEITSKGGRD
                     LVTATDTVVEDAIRAIVEGETPHPVVGEERGGEAPADGSSYWLLDPICGTRNFASGVP
                     LYCVNLALV"
     gene            complement(344..853)
                     /locus_tag="VIX62_06575"
     CDS             complement(344..853)
                     /locus_tag="VIX62_06575"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5859930.1"
                     /translation="MDAESLLAFVRFHAWANDRIMTTTAGLSEEDFRRAALLDHGTAF
                     QTLRHLVDVDWSWREFCTGNDIGQAYVWDLGFVLDDLPAIHAFSLKEDDRLRSYVESL
                     GDAGLNEPMKLSEDPDDVVPRWLILAHVVNHGTQHRSELARYLTECGHSPGELDLVDA
                     PRLPWPVER"
     gene            complement(895..1797)
                     /locus_tag="VIX62_06580"
     CDS             complement(895..1797)
                     /locus_tag="VIX62_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY5859931.1"
                     /translation="MLVREIPTEVPVDLARTLFPLRRGTGDPTMRIAPGSVWRATRTP
                     DGPATIHLALSGATAAIHAEAWGEGADWIIERAPGLAGLEDDASAFRPRDDVIRDLHR
                     RMPGVHLTRTDRLIEALIPAVLEQKVAGVEARRAYLRMTRSLAEPAPGPGALLLPPDP
                     ARVATLPYHAFHPFGLERRRAETLRFACAKAPAIEALAERPPAEAREYLGRFPGIGVW
                     TTAEVARLALADADAVSVGDYHVPNMVTWALAGEPRGTDERMLELLEPYRGQRGRVQV
                     LLEAGGITAPRFGPRMRTRAIERL"
     gene            1837..2895
                     /gene="rlmN"
                     /locus_tag="VIX62_06585"
     CDS             1837..2895
                     /gene="rlmN"
                     /locus_tag="VIX62_06585"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY5859932.1"
                     /translation="MHVYDLSLDQLTARLTAWGEPAFRAKQVYAQLWKRGATYEEMTD
                     VAPSLRERLATELPIEVELLNERAADKGATRKALLKLGDKGHVIEVVLMGYRDRVTVC
                     VSSQAGCAMGCGFCATGQMGLESNLTEGEIAAQVVWARREAARLPDTTPQRLSNVVFM
                     GMGEPMANYRAVRGSIDRITDPAGIRMGARHITVSTVGLVPGIKRLGQDHPQVGLAVS
                     FHAADDELRDDLVPPNRLYPLARLEAAMAAWRDRTHRRPSIEWAMIADVNDADEQAGK
                     LAPIAHRLGAHVNLIPLNPTPGWPSQPSSTARIEGFVQVLRAGGVNVTVRDTRGRRID
                     AACGQLRRDHDELTLHPV"
     gene            complement(2950..3600)
                     /locus_tag="VIX62_06590"
     CDS             complement(2950..3600)
                     /locus_tag="VIX62_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859933.1"
                     /translation="MKAPKRLLALAISLVILALIATMSLASTRSVTHDVPAGANTLIS
                     PDEPDEGDCSSDASACVDPTDEDADTTGDEDEDTGETPDPVANPDRGAACTTAAGIEP
                     TDGTDDAADTKLHGVDNAIAHVLANCVKNPQAPGLLNALEHLAANQARHDAHEAEKAE
                     RKAAHDAAKAQKHAGGPGHGKGHGSNGEHGNPHDGVHGHPHGDAGHGHGGSHGNGH"
     gene            3750..>4599
                     /locus_tag="VIX62_06595"
     CDS             3750..>4599
                     /locus_tag="VIX62_06595"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY5859934.1"
                     /translation="MGTRAAQAAVATWLAGVAAMALVGSAPASPLQPPLPAGDAGAGP
                     LAALMRAAFGERIASDDPAAVVASVLAIALVVVGFGLVARVAWRGELSLRATLVLAVI
                     GCVVVLAMPLLYSRDVYSYALYGRIAAFHHANPYVVTPAEVSTDPFFPLVGSQWQSTT
                     SVYGPVFTMLSVVLTRVFEGPAAVVAAFRALAVVSVLGAAALIARACLRVRPGVAAFA
                     VAVFALNPAVLFQTAGSGHNDAFVALSIAAAMAFVVSRRELAATVALVLGTLIKSTCG
                     VALVLWI"
BASE COUNT          604 a         1590 c         1675 g          730 t
ORIGIN      
        1 accagcgcca ggttgacgca gtacaggggg accccggacg cgaagttgcg ggtgccgcag
       61 atgggatcga ggagccagta cgaggacccg tccgccggag cttcgccgcc gcgctcctca
      121 cccacgaccg gatggggagt ctcaccctcg acgatggcac ggatcgcatc ctcgacgacc
      181 gtgtcggtcg ccgtcacgag atcgcggcca cctttggagg tgatctcgcc cgcgcccaca
      241 cggctcaggg cgaggtcgag tcctcgacgg acggcggtga tggcgatcgc ggtcgccggt
      301 tgcaggcctt cgacggcggg ttcgtcggcc aacgggtcgg cgcctaccgt tcgaccggcc
      361 atggcaggcg aggggcgtcc acgaggtcca gctcgccggg cgagtgcccg cattcggtga
      421 ggtaacgggc cagctcgctg cggtgctggg tcccgtggtt cacgacgtgc gcgaggatca
      481 accaccgcgg aaccacgtcg tccgggtctt cgctcaactt catcggctcg ttcagccctg
      541 catcgccgag agactccacg tagctccgca gacggtcgtc ctccttcagg ctgaacgcgt
      601 ggatcgccgg caggtcgtcg agcacgaacc cgagatccca cacgtaggcc tgaccgatgt
      661 cgttgccggt gcagaactcg cgccagctcc agtcgacgtc gacgaggtgg cggagcgtct
      721 ggaacgcggt gccatggtcg aggagcgccg cacgacggaa gtcctcttcc gacaggccgg
      781 cagtcgtcgt catgatgcgg tcgttcgccc atgcatggaa ccggacgaag gcaagcaagc
      841 tctcggcatc catcgaccaa ccctagcgtg cctggcgatg gcctacggtg ccggtcagag
      901 ccgttcgatg gctcgtgtgc gcatccgcgg gccgaaacgg ggggcggtga tcccgccggc
      961 ctccagcagc acctgcaccc ggccgcgctg tccgcgatag ggctccagga gttcgagcat
     1021 gcgctcgtcg gtgccccgag gttcgcccgc cagggcccag gtcaccatgt tggggacgtg
     1081 gtagtcgccc accgatacgg cgtcggcatc ggcgagcgcg aggcgcgcca cctcggcggt
     1141 ggtccacacg ccgatgcccg ggaaccgtcc gaggtactcc cgggcctccg cgggaggtcg
     1201 ctcggcgagc gcttcgatcg cgggcgcctt cgcgcaggcg aaccgcaacg tctcggcacg
     1261 ccgacgttcc aggccgaacg gatggaacgc gtgatagggg agcgtcgcca cccttgctgg
     1321 gtcgggtggc aggagcagag ccccggggcc gggggccggt tcggcgaggg agcgggtcat
     1381 ccggagatag gctcggcggg cttcgacgcc cgccaccttc tgctcgagga cggcggggat
     1441 cagggcctcg atcagacggt cggtccgggt gaggtggacg ccgggcatgc gccggtgcag
     1501 atcgcggatc acgtcatcgc gcggacggaa cgcgctcgcg tcatcctcga ggccggcgag
     1561 tccgggcgcg cgttcgatga tccagtctgc gccctcgccc caggcttccg cgtggatcgc
     1621 ggcggtggcg cccgataacg caaggtggat ggtcgcgggc ccgtccgggg tgcgcgtcgc
     1681 acgccagacc gaccccggcg cgatccgcat cgtgggatcg cccgtgccgc gccggagcgg
     1741 gaagagcgtg cgggccagat ccaccgggac ctcggtcggg atctcgcgca ccagcatcgc
     1801 ggccagccta gcgcccgccc gcgtaggctc gtgagcgtgc acgtctacga cctctccctc
     1861 gatcagctca cggctcgcct cacggcgtgg ggggaaccgg cgttccgggc caagcaggtg
     1921 tacgcccagc tctggaagcg cggcgccacc tacgaggaga tgaccgacgt cgcgccgagc
     1981 ctgcgcgaac ggctggccac cgaactgccg atcgaggtcg aactgctgaa cgagcgcgcc
     2041 gccgacaagg gcgcgacgcg caaggcgctg ctgaagctcg gcgacaaggg ccacgtgatc
     2101 gaggtcgtgc tgatgggcta ccgcgaccgc gtcacggtgt gcgtctcgtc ccaggccggg
     2161 tgcgcgatgg ggtgcggctt ctgtgccacg ggtcagatgg gactggagag caacctcacc
     2221 gagggcgaga tcgccgcgca ggtggtctgg gcgcggcgcg aggcggcccg gctgccagac
     2281 accacgccgc agcgcctctc caacgtggtg ttcatgggga tgggcgagcc gatggcgaac
     2341 tatcgagcgg tgcgcggctc gatcgaccgg atcaccgacc ccgcgggcat ccggatgggc
     2401 gcccggcaca tcacggtttc gacggtgggc ctcgtgccgg ggatcaagcg gctcggtcag
     2461 gaccacccgc aggtgggtct ggcggtcagc ttccacgccg ccgacgacga gctccgcgac
     2521 gacctggtgc cgccgaaccg cctctacccg ctggcgcgac tggaggcggc catggccgcc
     2581 tggcgcgacc ggacccaccg gcgcccctcg atcgagtggg cgatgatcgc cgacgtgaac
     2641 gatgccgatg agcaggcggg caagctcgcg cccatcgcgc accggctggg tgcgcacgtg
     2701 aacctgatcc cgctcaaccc cacgcccgga tggccgtcac agccgagctc gaccgcgcgg
     2761 atcgaggggt tcgtgcaggt cctgcgcgcg ggaggcgtga acgtgacggt ccgtgatacg
     2821 cggggccgca ggatcgatgc ggcgtgtggt cagctccggc gcgatcacga tgaactgacg
     2881 ctgcaccccg tgtgaggtcc ggcggcgggc cacgcccgca cgggcccgcc gccgttcctc
     2941 ctgcccggcc tagtggccgt tgccgtggga cccgccgtgc ccgtgcccgg cgtccccgtg
     3001 ggggtgaccg tggacgccgt cgtgcgggtt cccgtgctcg ccgttcgaac cgtgtccctt
     3061 gccgtgaccc ggtccgccgg cgtgcttctg tgctttggcc gcgtcgtgcg cggccttgcg
     3121 ttcggccttc tcggcctcgt gcgcgtcgtg cctggcctgg ttcgcggcga ggtgctccag
     3181 ggcgttgagc agcccgggcg cctgcgggtt cttcacgcag ttcgcgagca catgcgcgat
     3241 cgcgttgtcc acgccgtgga gcttcgtgtc ggcggcgtcg tccgtgccgt cggtgggctc
     3301 gatccccgca gccgtcgtgc aagcggcccc acggtcgggg ttggccacag gatccggggt
     3361 ctctcccgtg tcctcgtcct cgtctcccgt cgtgtccgca tcctcgtccg tggggtccac
     3421 gcacgccgag gcgtcggagg agcaatcgcc ctcgtccggt tcatcgggcg aaatgagtgt
     3481 gttggcccca gccggcacat cgtgggtgac cgatcgggtg ctggctaggc tcatcgtggc
     3541 gatgagcgcg aggatgacca gcgagatcgc cagcgcgagc agccgcttcg gagccttcat
     3601 gccgcccgta tcggcacgta cgtacctagt tcttgagcgc tacgtacgta tcacctgccc
     3661 ggttggagtc ccgaacgcgc cgtccggacc gcggcgtcgc ccgggtccct tgcgccgtga
     3721 cgaaagaccc tgctgctacg gtcgccgaga tgggaactcg ggcggcacag gcggcggtcg
     3781 cgacctggct cgcgggggtc gcggcgatgg ccctcgtggg ctcggcgccc gcttcgccgc
     3841 tccaaccgcc actgcccgcc ggcgatgccg gggccggtcc gctcgccgca ctgatgcgcg
     3901 ccgcgttcgg agagcgcatc gcttcggacg accctgcggc ggtggtggcg agcgtcctcg
     3961 cgatcgcgct cgtcgtggtg gggttcggcc tcgtggcgcg ggtggcgtgg cgcggagaac
     4021 tctccctgcg ggcgacgctc gtgctcgccg tgatcgggtg cgtcgtggtc ctggcgatgc
     4081 ccctgctgta ctcccgcgac gtgtacagct acgcgctgta cggacggatc gccgcgttcc
     4141 accacgcgaa cccgtacgtg gtgacgcccg ccgaggtcag caccgatccg ttcttcccgc
     4201 tggtggggag tcagtggcaa agcaccacat ccgtgtacgg gccggtgttc acgatgctct
     4261 cggtcgtgct gacccgtgtg ttcgaaggtc ccgcggccgt ggtcgccgcc ttccgcgcgt
     4321 tggccgtggt ctcggtgttg ggcgccgcgg cgctgatcgc gcgtgcctgc ctgcgcgttc
     4381 gccccggcgt cgccgcgttc gccgtcgccg tgttcgcgct caaccccgcc gtcctgttcc
     4441 agacggccgg aagcgggcac aacgatgcgt tcgtggcgct ctcgatcgcg gcggcgatgg
     4501 ccttcgtcgt ctcgcgccgg gaactggcgg cgacggtggc gctcgtgctg ggcacgctga
     4561 tcaagtcgac gtgcggcgtc gccttagtgc tctggatcg
//
LOCUS       DATDBZ010000269         3419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1722414, whole genome shotgun sequence.
ACCESSION   DATDBZ010000269 DATDBZ010000000
VERSION     DATDBZ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722414"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VIX62_06600"
     CDS             <1..389
                     /locus_tag="VIX62_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEY5859935.1"
                     /translation="KVVARIYDPRRAEIYERLNIPTVATTAWGIKQIQLMLFHDRKEI
                     RESLGGGDLLRMRVPVPPHLVGRSPSSLDVEGKVLVAGVSRGGGGFLPTDDSTMQAGD
                     FLVLMLTKDGVETMDQMLEAPSGDHA"
     gene            386..1105
                     /locus_tag="VIX62_06605"
     CDS             386..1105
                     /locus_tag="VIX62_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY5859936.1"
                     /translation="MKVIVTGGGAVGRHLAVDLVERGHEVTLIEQNHALADKLRSWAE
                     GVNIVFGDACEPWVLEEAQMAQAEVVVAATGDDEDNLVTSLLAKQEFAVPRVLARVNH
                     PKNEWLFNEQWGVDAAVSPPHILTAMVEEAVTVGDLVRLIRLEGGDISIVEMTLPEGS
                     SSVGKAIYELRLPADAAIVGILREGHVVIPQPETVLSAGDEVIALASAPSEHALRDAV
                     VGAGMPGGVPNDTIDGASGVV"
     gene            complement(1102..1833)
                     /locus_tag="VIX62_06610"
     CDS             complement(1102..1833)
                     /locus_tag="VIX62_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY5859937.1"
                     /translation="MRKTVAGCVVAVVATAALVVVLHPHRGDINTVSEAFAFLFVVVG
                     TVWLGGLWPGILASLLGFACFNYFFIPPFDTFAIGKSEDVVMLFAFLALSVVISLLVT
                     RSDARAAAAEARASELRMQQDLSRALVEPDTGPDRYAVVLRLIVSSFGFVDGVLFEQP
                     HADLGGLVEMTVVNAEPGTIATTAGDGVERFPLNVGRRNLGLLVLRGDRAPLTASERR
                     ILEAFGNQLALLLERDRLLRGATSA"
     gene            complement(1839..2516)
                     /locus_tag="VIX62_06615"
     CDS             complement(1839..2516)
                     /locus_tag="VIX62_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5859938.1"
                     /translation="MTSVLVVDDEPQILRGLRTNLRARGYEVFTAPDGEAALATAAKE
                     RPDLAIIDLGLPGIDGIEVIRGIRAWSEMPLLVLSARDQERDKVLALDAGADDYVTKP
                     FGMDELLARLRAAERRLHPSAEQPVVETDAFKVDLGAARVSTAEGDVRLTPTEWHLVE
                     ILVRNPGKLVSQRQLLQEVWGPQYQDETNYLRVYMAQIRRKLEPDPAEPRFFITEPGM
                     GYRFEPR"
     gene            complement(2513..>3419)
                     /locus_tag="VIX62_06620"
     CDS             complement(2513..>3419)
                     /locus_tag="VIX62_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5859939.1"
                     /translation="EGQAWVMESSAGPAPPTVSAEASDVIELKDGALLLLRGDRLPAE
                     SRRVLSAFAAHLSAAVRARDLSEEVAEAAELVEIDELRTAILQAVSHDLRTPLASIKA
                     AASSLRQDDVPWSEEETEEFLATIEEETDRLSVMVGNLLDMSRIQSGALNLVRVRVGL
                     DEVVPRALASLPDNGRAIRSEVPESLPRVEVDAALLERAIANIAGNAMAWTPAEEHVA
                     IVGSSGAHRVELRVIDRGPGIAPDDRDRVFEPFQRFGDRSSSDGVGLGLAVAKGFVEA
                     MGGDLTIEDTPGGGLTMVMSFSEAA"
BASE COUNT          553 a         1205 c         1155 g          506 t
ORIGIN      
        1 cgaaggtggt ggcgcggatc tacgacccgc ggcgtgccga gatctacgag cggctgaaca
       61 tccccacggt ggccacgacc gcatggggca tcaagcagat ccagttgatg ctgttccacg
      121 accgcaagga gatccgcgag agcctgggag gcggcgacct cctgcgcatg cgggtgccgg
      181 tgcccccgca cctcgtgggt cgctcgccct cttcgctcga tgtcgagggc aaggtgctcg
      241 tggccggggt ctcgcggggc gggggcggat tcctccccac ggacgacagc acgatgcagg
      301 cgggcgactt cctggtgctg atgctcacga aggacggcgt ggagacgatg gaccagatgc
      361 tcgaagcccc gagcggtgat cacgcatgaa ggtgatcgtg accggcggtg gggccgtagg
      421 acggcatctc gcggtcgacc tcgtggagcg cggccacgag gtgacgctaa tcgagcagaa
      481 ccacgcgttg gccgacaagc tgcggtcgtg ggccgagggc gtgaacatcg tgttcggcga
      541 cgcgtgcgag ccgtgggtgc tcgaggaagc gcagatggcc caggccgagg tcgtggtggc
      601 cgccaccggc gacgacgaag acaacctggt gacctcccta ctcgccaagc aggagttcgc
      661 ggtcccgcgc gtcctggccc gcgtcaacca ccccaagaac gagtggctct tcaacgaaca
      721 gtggggcgtc gatgccgcgg tctccccgcc gcacatcctc accgccatgg tggaggaagc
      781 cgtcaccgtc ggcgacctgg ttcggctgat ccggttggaa ggcggcgaca tctcgatcgt
      841 ggagatgacc ctgccggaag gttcgagctc ggtgggcaaa gcgatctatg aactgcggct
      901 gccagccgac gccgccatcg tgggcatcct gcgcgagggt cacgtcgtga tccctcagcc
      961 cgagacggtg ctctcggccg gcgacgaggt catcgcgctg gcgagcgctc cgtccgaaca
     1021 cgcgctccgc gacgcggtgg tgggcgccgg catgccaggc ggcgtgccca acgacacgat
     1081 cgatggcgcc agcggcgtcg tctaggcgga cgtcgcccca cgcaacagcc ggtcgcgttc
     1141 cagcagcaac gccaactggt tcccgaacgc ctcgaggatc cggcgctccg acgccgtgag
     1201 cggcgcgcgg tcgccacgca gcaccaacag gcccaggttc cgcctgccca cgttcagcgg
     1261 gaaccgttcc acgccgtctc cggcggtcgt cgcgatcgtg ccgggctcgg cgttgaccac
     1321 cgtcatctcc accaagcctc cgagatcggc gtgcggctgc tcgaacaaga ccccgtcgac
     1381 gaacccgaac gaactgacga tcagccgcaa caccaccgcg tatcgatcgg gtcccgtgtc
     1441 cggctcgacc agcgcgcgcg acaggtcctg ctgcatccgc agctcggagg cgcgcgcctc
     1501 ggccgcggcg gcacgcgcgt cgcttcgcgt caccagcaag gagatcacca ccgacagggc
     1561 gaggaacgcg aacagcatca cgacgtcctc gctcttgccg atcgcgaacg tgtcgaacgg
     1621 cgggatgaag aagtagttga agcacgcgaa ccccaggagc gaggccagga tgccgggcca
     1681 cagccctccg agccacacgg tgcccacgac cacgaacagg aacgcgaacg cctcggacac
     1741 cgtgttgatg tcaccgcggt gcgggtgcaa caccaccacg agcgccgcgg tggccaccac
     1801 cgcgaccacg cagccggcga ccgtcttcct catggtcctc acctcggttc gaatcggtag
     1861 cccatgcccg gctcggtgat gaagaaccgg ggctcggccg ggtcaggctc gagcttgcgc
     1921 cggatctggg ccatgtacac acgaaggtag ttcgtctcgt cctggtactg cggaccccac
     1981 acctcctgga gcaactggcg ctggctcacg agcttgcccg ggttgcgcac gaggatctcc
     2041 acgagatgcc actcggtggg cgtgagtcgc acgtcacctt cggcggtgct gacccgcgcc
     2101 gcacccaggt ccaccttgaa cgcgtcggtc tccaccacgg gttgctcggc ggaggggtgc
     2161 aatcgtcgtt cggccgcgcg gagccgcgcc aggagttcat ccatcccgaa cggcttcgtg
     2221 acgtagtcgt cggcaccggc gtcgagggcg agcaccttgt cgcgctcctg gtcgcgggcc
     2281 gacagtacga gcagcggcat ctcgctccag gcacggatcc cgcggatcac ctcgatgccg
     2341 tcgatgcccg gcaagccgag gtcgatgatc gcgaggtcgg gacgctcctt cgcggcggtc
     2401 gccaacgcgg cctcgccgtc gggcgccgtg aacacctcgt agccgcgggc gcgcaggttc
     2461 gtgcgcagac cacgcaggat ctgcggctcg tcgtcgacca ccaggacgct cgtcacgccg
     2521 cctccgagaa cgacatcacc atcgtgagtc cgccaccggg cgtgtcctcg atcgtgaggt
     2581 caccacccat cgcttccacg aatcccttcg cgaccgcgag cccgagaccc acaccgtccg
     2641 atgacgagcg gtcgccgaag cgttggaagg gttcgaacac ccggtcgcgg tcgtccggag
     2701 cgatccccgg ccctcggtcg atcacccgca gctcgacgcg gtgagccccc gagctgccga
     2761 cgatcgcgac gtgttcctcg gcgggcgtcc atgccatcgc gttgccggcg atgttggcga
     2821 tcgcgcgttc caacagggcg gcgtcgacct cgacccgtgg caggctctcc ggcacctccg
     2881 accggatcgc acgtccgttg tcggggaggc tcgcgagcgc gcgcgggacc acttcgtcca
     2941 agcccacccg gacgcggacg aggttgagcg ctccgctctg gatccggctc atgtcgagga
     3001 ggttgccgac catcacgctg agccggtcgg tctcctcctc gatcgtggcg aggaactcct
     3061 cggtctcctc ctcgctccac gggacgtcgt cctgacggag gctgctcgcc gcggccttga
     3121 tggatgcgag cggcgtccgc aggtcgtgcg agactgcctg caggatcgcg gtacggagct
     3181 cgtcgatctc gacgagctcc gcggcttcgg caacctcctc gctgaggtcg cgggcacgca
     3241 ccgccgcgga cagatgggcg gcgaacgcac tcagcacccg gcgggactcc gccggcaagc
     3301 ggtcgcctcg cagcagcagg agggcgccat ccttgagctc gatcacgtcc gatgcctctg
     3361 cggacaccgt cggtggggcg ggtccggcgg aggactccat cacccacgcc tggccctcc
//
LOCUS       DATDBZ010000270         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1092965, whole genome shotgun sequence.
ACCESSION   DATDBZ010000270 DATDBZ010000000
VERSION     DATDBZ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092965"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..1742
                     /locus_tag="VIX62_06625"
     CDS             375..1742
                     /locus_tag="VIX62_06625"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY5859940.1"
                     /translation="MRNRTILAIVVVFVVAAFPPTTTAAPQRALGAPRQGSPGDAVLD
                     WNANAGKAAIAACIAPTDNPLHESRMYAMMHVAIHDALNAIDRRSDPYVFHGRTRRPA
                     SPDAAVATAARDVLVPLLSELPAPFPQECIDAGVTSVEADYAAALGAIPDNAAKTRGM
                     RKGAMAAAAILALRASDGSDTPLVDSAYPQGTDPGEYRFTPGTPFAFAPGWADVTPFV
                     LRDASQFRAPPPYGVTSRRYTRDFRQVKRLGGDDTSTPSDRTNNQTEMALFWVESSPL
                     QWNRIARTVSAGRGLDMWENARLFGLVNLAMADGYIGSFETKYLYNFWRPVTAIQNAG
                     TDGNPNTNADPTWTPLVTTPPIPDYDSAHSVEGGAAAQVLRRFFGTDDISFETCSLTL
                     PAGSTCDDPSPVTRSFSSFSEAAEENAFSRVLVGFHFLKACEVGVQHGRTIGNRAINR
                     FLLPV"
     gene            1875..>2193
                     /locus_tag="VIX62_06630"
     CDS             1875..>2193
                     /locus_tag="VIX62_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5859941.1"
                     /translation="MSDHPITFAGDALERHAWKDAYEALIEADRTDPLPGAALEMLAW
                     ATYWTGHPHETIDALERAYAAYLDEGDRAQAAMMAYRLAEQHGMRMSIPLAQGWGQKA
                     ARLA"
BASE COUNT          358 a          769 c          727 g          339 t
ORIGIN      
        1 tcgtgaaccg gggcgagacg tacctcgacg accttgccga cgcgaagctg cgaggcccga
       61 tcggcgaggt cctgccgcgg ctggttgcat cggcggacga gcaacgccgc aggccggccg
      121 gctgattccg catgaaggac atcgcgaggc ccagagcttg acctcgggtg cctcgcaaga
      181 cgaaagcgct tcatccgcca cgagcccggt gcgccgacgt gtcggacgtt cttctgactc
      241 ccgcgtgacg gcgcccgccg ctatcgtctg ggggtcggta cttgccgcga ttcgcgtgaa
      301 gtgactgaca ggggtgcccg cctcctgggc tctgctccca cggatcgccg atgtccgcga
      361 tcggaggggg caccatgagg aaccgcacca tcctcgcgat cgtcgtcgtg tttgtcgtgg
      421 cggcgttccc accgacgacg acggcggctc cgcagcgggc cctcggcgca cctcgccagg
      481 gatccccggg agatgcggtc ctcgactgga acgcgaacgc gggcaaggca gcgatcgccg
      541 catgcatcgc tccgaccgac aacccgctgc acgaatcgcg gatgtacgcg atgatgcatg
      601 tggccatcca cgacgcgctg aacgcgatcg accgacggtc tgatccctac gtcttccacg
      661 gtcgaacgcg gcgtccggcg tctcccgatg ccgccgtggc cacggccgcg cgagacgtgc
      721 tcgttcccct gctgagcgaa ctccctgctc cattccctca ggagtgcatc gatgccggcg
      781 tcacgagcgt cgaggcggac tatgcggccg cgctcggtgc gatcccggac aacgcggcca
      841 agacacgtgg catgaggaag ggagcgatgg ctgctgcggc catcctcgcg ctcagagcct
      901 cggacggctc agacactccg ctggtggact ccgcgtaccc ccagggcacg gaccctggtg
      961 agtaccggtt cacgcccggc acgccgttcg ccttcgctcc cggctgggcg gacgtgaccc
     1021 ccttcgtgct ccgtgacgct tcgcagttcc gcgcgccccc gccctacggc gtgacgagca
     1081 ggcggtacac cagggatttc cgtcaggtga agcggctcgg cggcgacgac acctccacgc
     1141 ccagcgaccg cacgaacaac caaaccgaga tggcgctctt ctgggtcgag agctccccgc
     1201 tgcagtggaa ccggatcgcc cgaaccgtgt ccgccggcag gggcctcgac atgtgggaga
     1261 acgcgcgact gttcggcctg gtcaacctcg ccatggcgga cggttacatc ggctccttcg
     1321 agaccaagta cctctacaac ttctggcgtc ccgtcacggc gatccagaat gccgggacgg
     1381 acggcaaccc gaacacgaac gccgacccga cctggactcc gctggtgacg actccaccga
     1441 tccccgacta cgactccgca cacagcgtcg aaggaggagc ggccgcgcag gtgctgcgtc
     1501 gtttcttcgg gaccgacgac atcagcttcg agacctgcag cctgaccctt ccggccggga
     1561 gcacgtgcga cgacccgtcg cccgtgacgc gttcgttctc gagcttctcc gaagcggccg
     1621 aggagaacgc cttctcgcgg gtcctggtgg ggttccattt cctgaaggca tgcgaggtgg
     1681 gcgtccagca cggccgcacg atcggcaacc gcgccatcaa ccggttcctg cttcccgtgt
     1741 aggaggtgcc cggtaggcgt tcagacgtcg gacgagtcgc gagcgagctc atcgtgagga
     1801 ccgcccctct gcgcctccgc tcctgagcgg gtacggttcg atgtgcccgc tgcacctgtg
     1861 gagtcgccgt gagcatgagc gaccatccga tcaccttcgc gggggatgcg ctggagcggc
     1921 acgcctggaa ggacgcctac gaagcgctga tcgaggccga ccggaccgat cccctaccgg
     1981 gggcggcgct cgagatgctc gcctgggcga cttactggac gggtcatcca cacgagacga
     2041 tcgatgcgct ggagcgagcc tacgccgcat acctcgacga gggcgatcgg gcgcaggcgg
     2101 ccatgatggc gtaccgactc gcggaacagc acggcatgcg catgtcgatc ccgctggcgc
     2161 agggctgggg gcagaaggcc gcccgcctcg ccg
//
LOCUS       DATDBZ010000271         4708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1313936, whole genome shotgun sequence.
ACCESSION   DATDBZ010000271 DATDBZ010000000
VERSION     DATDBZ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313936"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="VIX62_06635"
     CDS             complement(<1..1526)
                     /locus_tag="VIX62_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019484148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEY5859942.1"
                     /translation="MTTTSARPVLDQAQLSRINAWWRAANYLSVGQIYLLDNPLLREP
                     LEPEHIKPRLLGHWGTTPGLNFIYAHLNRAIMARDLDVMYVMGPGHGGPGPVAASWLE
                     GSYTETYPNVTRDAEGMRRLFRQFSFPGGIPSHVAPETPGSIHEGGELGYALSHAYGA
                     AFDNRDLVVAAVVGDGEAETGPLAASWQSNKFVDPQRDGTVLPILHLNGYKIANPTVL
                     ARISPKELDHLLRGYGHTPYYVEGDDPAQMHQAFARALEACLDKIKEIKRRAREEGRV
                     ERPLWPMIVLRSPKGWTGPAEVDGQKVEGTWRSHQVPFAEARENDSHRDVLERWLRSY
                     RPEELFDQSGAPVPAIASLHPSGTRRMSSNPHSNGGGLLRDLRMPDFREYGVPVLEPG
                     ASNAESTRALGVLLRDVMAQNMDNFRVFAPDENNSNRFNAVLEVTNRAWNAATIPGDD
                     HLAPDGRVMEILSEHTCQGWLEGYLLTGRHGFFSCYEAFIHIIDSMFNQHAKWLTTTN
                     "
     gene            complement(1552..4119)
                     /locus_tag="VIX62_06640"
     CDS             complement(1552..4119)
                     /locus_tag="VIX62_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY5859943.1"
                     /translation="MNHTQSVVLEISGVQWASSKAVAEATLLRRPGVISVEANPVSQT
                     ANITYDPHATNVAELRDWVEDCGFHCAGQSVPGHICDPMEEPANRVTAPATGAHAEQD
                     DMAGDHTMHSASEHAGHGPALAESPPEASPAHEHGAHDHGAAGGQNVTAPPAATAHEH
                     APRTAQDVMGHGGHGAGASMDEMVRDMRNRFLVAAILSIPIVLWSPIGRDVFGFTVPA
                     PFGLRDDVFSLILSLPVIFYSAWIFFDGAYRALRARTLDMMVLVAVGVGAGWLYSVVI
                     TLTGGGEVFYEAATVLAAFVLLGHWVEMRARGGANDAIRTLLELAPPRAVVIRDGEPV
                     EVSTSEVVPGDLMLVRPGAKVPTDGVVEDGDSEIDESMVTGESLPVVKRPGNEVIGAT
                     VNTTGTLRVRATKVGADTALAQIVALVQEAQNSKAPGQRLADRAAFWLVLVALIGGTL
                     TLVVWLLAGATFQAALLFAITVVVITCPDALGLATPTAIMVGTGLGAKRGVLFKNATA
                     IESAAGIDTVVLDKTGTLTKGEPEVTDYIPIGYDGLELLALAAAAERESEHPLAKAVV
                     AYAEQRGARKLAAESFRNITGQGAVSTVDDHRVLLGNTRLMAAEGVDTAAVSDQQEVL
                     ASTGRTAIIVAVDGMAAGIIALADAPRETAAAAITAMHEMGVEVVMLTGDNTPTATRI
                     GQQLGIDTIIAEVLPEDKSAKIAELQKAGKKVAMVGDGVNDAPALAQADLGIAIGAGT
                     DVAIETADVVLMRSDPLDVPIALRIGKGTVRKMRQNLGWAIGYNAVALPIAAGVFYPA
                     FGIALSPEIAAISMSGSSVIVAVNALLLKRLRLPAAPAAASPVGPSREPVTPTPVVR"
     gene            complement(4210..4698)
                     /locus_tag="VIX62_06645"
     CDS             complement(4210..4698)
                     /locus_tag="VIX62_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEY5859944.1"
                     /translation="MMQTSEPSSDAVFNSFDVMFAQMMVPHHEQAIEMADAMLEKDAI
                     DPRVIDLDEQVKAAQQPEIDQLDEWLDEWGADPAPTGAMSRGSVMMSDTDVAAPDTAT
                     GAEASRVFLEQMTVHHEVAITMAQSEIDGGANPDAIELAQAIVDAQTEEITLMRDILD
                     SL"
BASE COUNT          724 a         1538 c         1598 g          848 t
ORIGIN      
        1 tcgttcgtgg tcgtcaacca cttggcgtgc tggttgaaca tcgagtcgat gatgtggatg
       61 aacgcttcat agcaggagaa gaaaccatgc ctcccggtga gcaggtaccc ctccagccag
      121 ccctgacagg tgtgttctga gaggatctcc atcacgcggc cgtcgggtgc cagatggtcg
      181 tcgcccggga tggtggccgc gttccatgct cggttggtca cctcgagtac ggcgttgaat
      241 cggttggagt tgttctcgtc gggtgcaaac acgcggaagt tgtccatgtt ctgtgccatg
      301 acgtcgcgca ggagaactcc gagggcgcgg gtggattccg cgttcgacgc tcccggctcg
      361 aggacgggga ccccgtattc acggaagtcg ggcatccgca ggtcccgcaa caggccgccg
      421 ccgttgctgt gcgggttcga gctcatgcgc cgcgttcccg acgggtgaag tgacgctatg
      481 gccggcacgg gagcaccgct ctgatcgaac agttcttcgg gtcggtagct gcgcagccat
      541 cgctccagga cgtcgcggtg cgagtcgttc tcgcgcgcct cggcgaaggg cacctggtgt
      601 gaacgccacg tcccttcgac cttctggccg tcgacctcgg ccggcccggt ccaccccttg
      661 ggcgagcgca acacgatcat cggccagagg ggccgctcga cccgcccttc ctcccgtgcc
      721 cggcgcttga tctccttgat cttgtcgagg caggcctcga gtgctcgggc gaaggcctga
      781 tgcatctgcg ccgggtcgtc tccctccacg tagtacgggg tgtggccgta accgcgcaag
      841 agatggtcca gctctttcgg gctgatcctc gccaggacgg tcgggttcgc gatcttgtag
      901 ccgttcagat gcaggatcgg cagaacggtc ccatcccgct gcggatccac gaacttgttc
      961 gactgccagc tcgccgccaa cggccccgtc tcggcctcgc catcgccgac caccgctgcg
     1021 acgacgaggt cgcgattgtc gaaggcagcc ccgtaggcgt gggagagtgc atatcccagc
     1081 tctccgccct catggatgga gccaggggtc tcgggagcga catggctggg gatgccgccg
     1141 gggaaggaga actgccggaa caggcgccgc atcccctcgg cgtcccgggt gacgttcggg
     1201 tacgtctccg tgtacgaacc ttccaaccag gatgcggcga cggggcccgg gcccccatgg
     1261 ccgggaccca tgacgtacat cacgtcgagg tcgcgcgcca tgatcgcccg attgaggtga
     1321 gcgtagatga agttcagtcc cggtgtcgtg ccccaatgcc cgagcagtct cggcttgatg
     1381 tgctcaggct cgaggggctc gcgcagcagc ggattgtcga ggaggtagat ctgcccgacc
     1441 gacaggtagt tggccgcgcg ccaccaggcg ttgatcctgc tcagttgcgc ttgatcgagg
     1501 acaggcctgg ccgaggtggt agtcatgagt cggatccctt cgttgattcg gtcaccgcac
     1561 cactggggtg ggtgtgacgg gctcccggct gggacctacc ggcgacgccg cggccggagc
     1621 cgcaggaagg cgcagtcgct tgaggaggag tgcgttcacg gcgacgatca cgctcgagcc
     1681 cgacatcgag atcgcggcga tctcggggct cagcgcgatc ccgaatgccg gatagaacac
     1741 accggcggcg atgggcagcg cgaccgcgtt gtagccgata gcccagccga ggttctgccg
     1801 catcttgcgt acggtgccct ttccgatgcg gagcgcgatc gggacgtcga gtgggtcgga
     1861 acgcatgagc acgacgtcgg cagtctcgat ggcgacatcg gtgccggcac cgatcgcgat
     1921 gccgaggtcg gcctgcgcca aggcgggcgc gtcattcaca ccgtcgccga ccatcgcgac
     1981 tttcttgccc gccttctgca gttcggcgat cttggccgac ttgtcctcgg gaaggacctc
     2041 ggcgatgatc gtgtcgatgc cgagttgctg tccgatgcgc gtggcggtcg gggtgttgtc
     2101 gccggtgagc atcaccacct cgacccccat ctcgtgcatc gcggtgatcg cggctgcagc
     2161 tgtctctcgg ggggcgtcgg cgagggcgat gatcccggcg gccatgccgt cgacggcgac
     2221 gatgatggcg gtacggccgg tgctcgccag cacctcctgt tggtcgctga cggctgccgt
     2281 gtcgacgcct tctgcagcca tcaggcgcgt gttgccgagc aacacgcggt ggtcgtcgac
     2341 tgtcgagacg gcgccttggc cggtgatgtt gcggaatgat tccgctgcga gctttcgcgc
     2401 accacgctgc tcggcgtagg cgacgaccgc cttggcgagc ggatgctccg actcccgctc
     2461 cgcggccgca gcgagtgcga gcagctccag cccgtcgtag ccgatcggga tgtagtctgt
     2521 gacctcgggt tcgcccttgg tgagggtgcc ggtcttgtca aggacgacgg tgtcgatgcc
     2581 cgccgccgac tcgatcgccg tcgcattctt gaagagcacg ccccgcttcg cgcccaggcc
     2641 ggtgccgacc atgatcgccg tgggggtggc cagaccgagg gcgtcggggc aggtgatcac
     2701 cacgacggtg atcgcaaaca ggagtgccgc ctggaacgtg gcacccgcga gcaaccagac
     2761 cacgagcgtc agcgtgccgc cgatgagtgc gaccagcacc agccagaacg cggcccgatc
     2821 tgcgagtcgc tgccccggcg ccttggagtt ctgcgcctcc tgcaccagcg caacgatctg
     2881 cgccaacgcc gtgtcagcgc cgaccttcgt ggcgcgtacc cgaagggttc ccgtggtgtt
     2941 cacggtcgcg ccgatgacct cattgccggg cctcttgacg accgggagac tctcgccggt
     3001 caccatggac tcgtcgatct ccgagtcgcc gtcctcgacc acgccatcgg tggggacctt
     3061 ggccccggga cgcacgagca tcaggtcgcc aggcacgacc tccgaggtcg acacctcgac
     3121 gggttcgccg tcacggatca cgaccgcgcg cggcggcgcg agctccaaga gggtgcggat
     3181 ggcgtcgttc gcaccgccgc gagctctcat ctcaacccaa tgcccgagca gtacgaacgc
     3241 ggcgagcaca gtcgcggcct cgtagaagac ctcgccgcca ccggtcagcg tgatcaccac
     3301 gctgtacaac cagccggcac cgacacccac cgcgacgaga accatcatgt cgagcgttcg
     3361 cgcccgcagg gcccggtagg cgccgtcgaa gaagatccaa gccgagtaga agatcaccgg
     3421 cagcgacagg atgagcgaga agacgtcatc ccgcagaccg aacggtgccg gcactgtgaa
     3481 gccgaacaca tcacgaccga tcggagacca caacacgatc gggatcgaca ggatcgcggc
     3541 gacgaggaac cggttgcgca tgtcgcgcac catctcgtcc atgctcgccc cggcgccgtg
     3601 accgccgtga cccatcacat cctgcgccgt ccgcggagcg tgctcgtgcg cggtcgcggc
     3661 ggggggagcc gtgacgttct gcccgcccgc tgcgccgtgg tcgtgtgcgc cgtgctcgtg
     3721 cgcgggtgac gcttccgggg gagattccgc cagtgccggc ccgtgccctg cgtgctcact
     3781 cgcggagtgc atcgtgtgat cacccgccat gtcgtcctgc tcagcatggg cgccagtcgc
     3841 tggtgcggtc acccggttcg cgggctcctc catggggtcg cagatgtggc cgggcaccga
     3901 ttggccggcg caatggaagc cgcagtcctc gacccagtcg cggagttccg cgacgttcgt
     3961 cgcgtggggg tcatacgtga tgttggcggt ctgtgagacg gggttggcct cgacgctgat
     4021 cacgccgggc cgccggagga gagtcgcctc cgcgacggcc ttggatgacg cccattggac
     4081 gcccgagatc tcgagtacga ccgattgtgt gtggttcatg agcagtgcgt cccttcgtcg
     4141 atgtgccgac tgatgaaact cagtgcggct tcgcgtgcga gctttcacgt ttgagcattc
     4201 ggggcggagt cagagggaat cgaggatgtc gcgcatgagc gtgatctctt ccgtctgtgc
     4261 gtcgacgatg gcttgggcca gttcgatggc gtcggggttc gcgcccccgt cgatctcgct
     4321 ctgggccatg gtgatcgcga cttcgtggtg cacggtcatc tgctcgagga acacgcggct
     4381 ggcttcggct ccggtcgcag tgtcgggcgc ggccacgtcg gtgtcggaca tcatgacgct
     4441 cccacggctc atggcacctg tcggggcggg gtctgcgccc cactcgtcca gccactcgtc
     4501 gagctggtcg atctcgggct gctgggcggc cttgacctgc tcgtcaaggt cgatcacacg
     4561 cgggtcgatg gcgtcctttt ccagcatggc atcggccatc tcgatggcct gttcgtggtg
     4621 cggcaccatc atctgcgcga acatcacgtc gaacgaattg aacacggcat ccgaactcgg
     4681 ctcgctggtt tgcatcatgg atcccccg
//
LOCUS       DATDBZ010000272         3078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2320990, whole genome shotgun sequence.
ACCESSION   DATDBZ010000272 DATDBZ010000000
VERSION     DATDBZ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320990"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VIX62_06650"
     CDS             complement(<1..165)
                     /locus_tag="VIX62_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY5859945.1"
                     /translation="MVDSACIFCRIAAHDIPADIVRESDHVVAFRDQNPQAPTHILLI
                     PKEHVESVAEI"
     gene            complement(180..1307)
                     /gene="dnaJ"
                     /locus_tag="VIX62_06655"
     CDS             complement(180..1307)
                     /gene="dnaJ"
                     /locus_tag="VIX62_06655"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY5859946.1"
                     /translation="MAAVRDLYEVLGVERGASDEDIKKSYRKLARELHPDVNADGDAE
                     ERFKEVAGAYEILSDPEKRQRYDAFGTTGGPQGAPFSDIQDIFDLFFGQGGGFGQGGF
                     GPSARRRGPRSRAQHGEDLGVHLTLGFREAVFGTREELDIERLVECERCMGNGAEPGT
                     APVACRTCGGAGQVQAVRKSVFGTVMTAAPCGTCRGTGQEVTDPCESCHGHGRRAEHA
                     TVTVDIPAGVSEGMELRVTGNGHAGVQGGPAGDLYVGLSVEPSIAFERRGQDLFTVLD
                     ISMTQATLGAEVQIEALDDTEHLRVEPGTESGTVIKLRGKGVPNVNRRGRGDLYVTMH
                     VITPGELSKEERELWQRLSVLRGEESSKRDPAVGELRRPEF"
     gene            complement(1311..2360)
                     /gene="hrcA"
                     /locus_tag="VIX62_06660"
     CDS             complement(1311..2360)
                     /gene="hrcA"
                     /locus_tag="VIX62_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY5859947.1"
                     /translation="MPERERPELGPRKDAVLRAVVEQYVRTGEPVGSETIAENAGLGV
                     SSATIRNEMAALEELGYLKHPHTSAGRIPTDVGYRHYVDSLPAGGRLREAQRRLIAGY
                     FAEAIVDLEEVLKGSVQLLSRLTQYAGLAVPPSANEEPVVRLELIDMGPTVMILAVGQ
                     HGRVDKHVIDRPDGVDTQTLARVERRLQALRGKTYLVAQARLLQLAAESSKEEHDLLL
                     HVAELLRVATQGEGSTHVVVGGASNLADEAQQMRRETLRRLFETLERETEMLAVLEDI
                     GSGQTALNVTIGAEHPSTQEWDASIITAPFKTGDTTIGTIGVVGPTRMDYLSAMASVR
                     VVAKRLSEMATELDQ"
     gene            complement(2397..2891)
                     /locus_tag="VIX62_06665"
     CDS             complement(2397..2891)
                     /locus_tag="VIX62_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859948.1"
                     /translation="MAIRGDDLDAAEPEPLRGPQSPFSDHDPAFPMPRRADRHGGVPA
                     WAWVALGAVVVAIIAIFVFTRGGDDPVDAPAPGSTAAMEQLCSDVGQLQQVIRTDALA
                     RAQDDLKADATALKEAGDKATAKQVKQLIAAVGDLRTALEDQQDTTEATNAMAEAIDA
                     VGCS"
BASE COUNT          479 a         1062 c         1001 g          536 t
ORIGIN      
        1 gatctcggcg accgactcca catgctcctt ggggatcagc aagatgtggg tcggagcctg
       61 agggttctga tcgcggaacg cgaccacatg atcggactcg cgaacgatgt ctgccgggat
      121 gtcgtgggcc gcgatcctgc agaagatgca tgccgagtcg accatgcaac aagcctacgt
      181 cagaactcag gacgccggag ttcgccgacg gccgggtcac gcttggacga ctcctcaccg
      241 cggagcaccg agagccgctg ccagagctcc cgttcctcct tcgagagttc gcccggcgtg
      301 atgacgtgca tcgtgacgta cagatcaccg cggcctcgcc ggttcacgtt gggtacgccc
      361 ttgcccctga gcttgatgac ggtgcccgat tccgtgcccg gttcgacccg gaggtgctcg
      421 gtgtcatcga gagcttcgat ctgcacctcg gcacccagcg tcgcctgcgt catggagatg
      481 tcgagcaccg tgaacagatc ctgcccgcgc cgttcgaacg cgatcgaggg ttcgaccgac
      541 aggcctacat acaggtcgcc cgccggccca ccctgcaccc cggcgtgtcc gttcccggtg
      601 acccgcaact ccatgccctc ggagacgccc gcggggatgt cgaccgtgac ggtcgcgtgc
      661 tcggcccgtc ggccgtgacc gtgacaggac tcgcacgggt cggtcacctc ctggcccgta
      721 ccccgacagg ttccgcatgg agccgccgtc atcaccgtgc cgaagacact cttgcgaacc
      781 gcctgcacct gacctgcgcc gccacatgtg cgacacgcga ccggcgcggt gccgggttca
      841 gcaccgttac ccatgcaccg ctcgcactcc accaagcgct cgatgtcgag ttcctcgcgg
      901 gtcccgaaca cggcctcgcg gaaccccagc gtcaggtgca caccgaggtc ctcaccgtgc
      961 tgggcacggg aacgcggacc acgacggcga gcgctggggc cgaacccacc ctggccgaac
     1021 ccacccccct ggccgaagaa caggtcgaag atgtcctgga tgtcggagaa cggtgcccct
     1081 tgcggtcctc ccgtggtgcc gaaggcgtcg tatcgctgcc gcttctcggg gtcggacaag
     1141 atctcgtagg cgcccgcaac ctccttgaac cgttcctcgg cgtccccgtc ggcgttcaca
     1201 tcggggtgga gttcgcgcgc gagcttccgg taggacttct tgatgtcctc gtcggaagcg
     1261 ccgcgttcca cgccgagcac ctcgtacaga tcgcgaaccg ctgccatggg ctactgatcc
     1321 agctccgtcg ccatctcgga caaccgcttc gccaccacgc gcaccgacgc catggccgac
     1381 aggtagtcca tgcgcgtggg accgaccacg ccgatcgtcc cgatcgtggt gtcgccggtc
     1441 ttgaacggcg ccgtgatgat cgacgcgtcc cactcctggg tcgaggggtg ttcggcaccg
     1501 atcgtcacgt tcaacgccgt ctgacccgag ccgatatcct cgaggaccgc cagcatctcg
     1561 gtctctcgct cgagcgtttc gaagagccgg cgaagggttt cacgacgcat ctgctgcgct
     1621 tcgtcggcga ggttggaggc cccaccgacc accacgtgcg tcgagccctc gccctgcgtc
     1681 gcgacacgga ggagctcggc gacgtggagc agcaggtcat gttcttcctt ggaactctcg
     1741 gccgcgagtt ggagcaaccg cgcctgcgcg acgagatacg tcttgcctcg caacgcttgg
     1801 agacggcgct cgacgcgtgc gagcgtctgt gtgtcgacgc catcggggcg gtcgatcacg
     1861 tgcttgtcga cccggccgtg ttggcccacg gcaaggatca tgacggtcgg ccccatgtcg
     1921 atgagctcta gccggacgac cggctcttcg ttggcgctgg gtggcacggc gagacccgcg
     1981 tactgggtca ggcgcgacag caactggacg ctgcccttga gtacctcctc caggtcgacg
     2041 atggcttcgg cgaagtagcc agcgatcaat cggcgctggg cctcgcggag gcggccacct
     2101 gccggcagtg agtccacgta gtggcggtac cccacgtcgg tggggatgcg acccgcgctg
     2161 gtgtgcgggt gcttcaggta cccgagttct tcgagtgcag ccatctcgtt gcggatcgtg
     2221 gcgctggaca caccgagtcc ggcgttctcg gcgatcgttt cgctgccgac cggttcgccg
     2281 gtgcgcacgt actgctcaac gacagcacgc agcaccgcgt ctttgcgggg accgagctca
     2341 ggacgttcac gttctggcat ggacccatga tacgtagcac tcaggttcgt cgagtgctag
     2401 ctgcagccga ccgcgtcgat ggcttcggcc atggcgttcg ttgcttccgt ggtgtcctgc
     2461 tggtcctcca gcgccgtgcg gagatcgccg accgctgcga tcagctgttt gacctgcttc
     2521 gccgtggcct tgtcaccggc ttccttcagg gcggtggcgt cggccttcag gtcgtcttgg
     2581 gctcgagcca aggcgtcggt gcggatcacc tgctgcaact gtccgacgtc ggagcagagc
     2641 tgctccatcg cagctgtgga cccgggtgcg ggtgcatcca ccgggtcatc cccgccgcgg
     2701 gtgaacacga agatcgcgat gatcgccacc acgacggcgc ccaacgccac ccacgcccac
     2761 gcagggacgc ctccatggcg gtcggcccgc cgcggcatag ggaacgcagg atcgtgatcg
     2821 ctgaacgggg actgcggacc cctcagtggc tcgggttcgg cggcgtcgag gtcatcgccc
     2881 cggatcgcca tcgacggagc gggcacctgc ggagtggtcg cctccggggt cgcgtgcgac
     2941 gcccctccga cggaggttcc gtggggcgtg ccgcagttcg ggcacgtcca ccccgtggat
     3001 ccgggcctgc catggaagcg tgaaccacag gtcgagcagg tcacgaacgt ccacgacgcg
     3061 ccacaagaag cgcatgtg
//
LOCUS       DATDBZ010000273         5599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2139487, whole genome shotgun sequence.
ACCESSION   DATDBZ010000273 DATDBZ010000000
VERSION     DATDBZ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139487"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VIX62_06670"
     CDS             complement(<1..636)
                     /locus_tag="VIX62_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5859949.1"
                     /translation="MSHAPALEVSRLAFAYPDGTQALFGIDLTIGVGERVALLGPNGA
                     GKTTLVMHLNGINTAQHGSVLVGGLPVTKQHMKEIRRRVGIVFQDPDDQLFMPTVRAD
                     VAFGPANMGVRGDDLDACVERALQAVGMEEFIDRPPHHLSFGQRRRVAVATVLAMEPE
                     ILVLDEPSSNLDPAGRRELADILRSLPITMLMVTHDLPYALELCQRAVVMNE"
     gene            complement(633..1394)
                     /gene="cbiQ"
                     /locus_tag="VIX62_06675"
     CDS             complement(633..1394)
                     /gene="cbiQ"
                     /locus_tag="VIX62_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEY5859950.1"
                     /translation="MSGAHALFVHGDSLIHRLRPECKLLAQVLFVFAVVATPREAFWA
                     FGVYVVMLVVLTQLADLTVAFVAKRLVIEVPFVLFAVFLPLIGQGQRIEVFGLSLSVE
                     GLWAMWNILIKGTIGVAASVILAATTPVPDFLRGLERLHLPSVFTSITGFMIRYSEVI
                     SQEARRMKVARESRGYDARWLWQAKGFAASAGALFIRSYERGERVHLAMISRGFDGRL
                     PEVDDGRAGTAQWAAAMSLPAVATLICVLAWVVRP"
     gene            complement(1391..1753)
                     /locus_tag="VIX62_06680"
     CDS             complement(1391..1753)
                     /locus_tag="VIX62_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HEY5859951.1"
                     /translation="MAAKRTTTWAVVVGGLLVALGLAFVVSRFASSSPDGLNKVAIDQ
                     GFDDTETNHATADGPFAGYGVKGVDDQGLSTGLAGIVGVVITFGIGLLLFVVVGRRKA
                     TGGERDAAAATASSGSAP"
     gene            complement(1753..2448)
                     /locus_tag="VIX62_06685"
     CDS             complement(1753..2448)
                     /locus_tag="VIX62_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEY5859952.1"
                     /translation="MHIPDGFINAGVSAAAGVVSAGGIGYSLVKAKETLSEKLAPMAG
                     LVAAFIFAVQMLNFPVAAGTSGHLLGGCLAAILVGPYAGAVAVAVVLVVQALLFADGG
                     LTALGLNVLNMALITSFGGYAIFRLIRGVLPANRGSVVAASGIAAVLSVVLAALGFVV
                     EYAIGGNGGASITTVAAAMLGTHLLIGIGEGIITALTIGVVLGVRPDLVYGAQDLLPT
                     LQLRDPSIVAGRP"
     gene            complement(2581..4233)
                     /locus_tag="VIX62_06690"
     CDS             complement(2581..4233)
                     /locus_tag="VIX62_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY5859953.1"
                     /translation="MTDARQRTQPTDEAVAAERSHRTLSGEPVPEIATAEDLASWDPD
                     ERLGRPGEFPFTRGVYPSMYRGRLWTMRQFAGFGTPAETNARYRFLLAQGQGGLSVAF
                     DMPTLMGLDSDDPRSEGEVGRCGVATDSLDDFETLFDSIPLGDISTSMTISGPATVAF
                     AFFLATAERQGVAWDQLDGTLQTDILKEYIAQKEWIFPPRPHLRLIGDLMAFCAERCP
                     KFHPVSVSGYHIREAGATAAQELAFTLADGFGYVELGQRRGLDVDRFSSGLSFFFNSH
                     IDFFEEIAKFRAARRIWARWLRDRYGATDPAAMRLRFHTQTAGVSNTAQQPMNNVVRT
                     AVEALAAVFGGTQSLHTNALDEVLALPTEDAAKLALRTQQVIAYETGAPDTIDPLGGS
                     YFVETMTDRIEAQAEEIFAHIDAMGEGSMLDGVLAGIEQGWFQQRIADAAFEEQRRYE
                     TGDLVKVGVNAFVETDEAPIDTLVIGPEAEVEQRVAVERTRAERDDAVAKAALADLVR
                     AAGTDENLIDPMVECARTRCTEGEIVSALVGVFGDYRETPRF"
     gene            complement(4276..5211)
                     /gene="meaB"
                     /locus_tag="VIX62_06695"
     CDS             complement(4276..5211)
                     /gene="meaB"
                     /locus_tag="VIX62_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017615212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY5859954.1"
                     /translation="MEVDELVRGVLARERRAVARAISAVEDGDADLESLSAGLYPHTG
                     SAATIGLTGAPGVGKSTLAAGLVGAARTRDLPVAVLAVDPTSPYTGGALLGDRVRMQE
                     HATDPGVFIRSMATRGHLGGMALAAPEAVRILDAAGYERVVVETVGVGQAEVDVAAAT
                     DTAVVVLAPGLGDAVQMAKAGILEVADIFVVNKADRDGAPEVVRELRQMLHLGAARDW
                     DPPVLTASASQGEGIEELWEAIESHRDHLAASGALAAKRRSRLIREVESLAAERFRLR
                     AAEALRADPQLADELEARRLDPYRAAAMLASRAAE"
     gene            complement(5222..>5599)
                     /locus_tag="VIX62_06700"
     CDS             complement(5222..>5599)
                     /locus_tag="VIX62_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859955.1"
                     /translation="KGLPLLAFVGIAVWLARRRERRWLQASLSPEVGLDGISAEELQV
                     LASPKRRRAARAQMRRRAGPRASSLLHRLQREQVNLAMVASRVPDDQDPALMRQREYC
                     RSLRDALQAIPGAAPAQTASGGG"
BASE COUNT          901 a         1993 c         1927 g          778 t
ORIGIN      
        1 ttcgttcatc accacggctc gctggcagag ctccagcgca taggggagat cgtgggtcac
       61 catcagcatc gtgatcggca gggagcgcag gatgtcggcg agctcgcgcc ggccggcggg
      121 gtccaggttc gaggacggtt cgtcgagcac caggatctcg ggctccatcg ccaggaccgt
      181 ggcgaccgcc acgcgccgcc gctgtccgaa gctcagatgg tgcggcggcc ggtcgatgaa
      241 ctcctccatg ccgacagcct gcaatgcgcg ctcgacgcac gcgtcgaggt cgtcaccgcg
      301 cacgcccatg ttggccggcc cgaacgccac gtcggcgcga acggtgggca tgaacagctg
      361 atcgtcaggg tcctggaaca cgatgccgac gcgcctccgg atctccttca tgtgctgctt
      421 ggtcaccggc aggccgccca ccagcaccga gccgtgctgg gccgtgttga tgccgttcag
      481 gtgcatcacg agcgtggtct tgccggcgcc gttcggaccc aggagcgcga cgcgctcgcc
      541 gaccccgatc gtgagatcga tcccgaacaa cgcctgggtg ccgtcgggat aggcgaaggc
      601 gagccgcgaa acctcgagcg ccggtgcgtg gctcacggcc gcaccaccca agcgagcacg
      661 cagatcaggg tcgccacggc cggcaggctc atggcggccg cccactgcgc cgtgcctgcg
      721 cgaccgtcat cgacctcggg gagacgaccg tcgaagccgc gggagatcat ggccaggtgc
      781 acccgttcgc cgcgctcgta ggaccggatg aacagcgcgc cggccgaggc cgcgaacccc
      841 ttcgcctgcc agagccaccg ggcgtcgtag ccacgggatt cccgcgccac cttcatgcgc
      901 cgggcctcct gcgagatgac ctcggagtac cggatcatga accccgtgat cgacgtgaac
      961 accgacggca ggtggagacg ttccaatccg cggaggaagt ccgggacggg cgtggtggcc
     1021 gccaggatca cgctggccgc gacgccgatc gtgcccttga tcaggatgtt ccacatcgcc
     1081 cacagacctt ccaccgacag ggatagaccg aatacctcga tccgctgacc ctggccaatc
     1141 aacggcagga acacggcgaa cagcacgaac ggcacctcga tcaccaggcg cttggcgacg
     1201 aacgccaccg tgagatcggc gagctgggtg agcaccacca gcatcacgac gtagacgccg
     1261 aacgcccaga acgcctcgcg gggcgtggcg acgacggcga acacgaacag gacctgggcc
     1321 agcaatttgc actccggccg gagccggtgg atcaggctgt cgccgtggac gaagagcgcg
     1381 tgggcgccac tcacggtgcg gagccggacg aggcggtcgc cgccgcggca tcgcgctcgc
     1441 cacccgtcgc cttgcggcga ccgacgacga cgaagagcag gaggccgatg ccgaaggtga
     1501 tgacgacgcc gacgatgccg gcgagcccgg tggacaggcc ctggtcatcc acacccttca
     1561 cgccgtagcc ggcgaatggg ccgtcggccg tggcgtggtt cgtctcggtg tcgtcgaacc
     1621 cctggtcgat cgcgaccttg ttgagcccgt cgggcgagct gctcgcgaac cggctcacga
     1681 cgaaggccag cccgagcgcc acgaggagcc cgcccaccac gaccgcccag gtggtggttc
     1741 gcttcgcggc catcatggcc tccccgccac gattgaggga tcgcgcagct gcagggtggg
     1801 gagcagatcc tgggcgccgt agacgaggtc gggacgaacg cccaggacca cgccgatggt
     1861 gagcgccgtg atgatgccct caccgatgcc gatcaggagg tgcgtgccca gcatggccgc
     1921 cgccacggtg gtgatcgacg cgccgccgtt gccgccgatc gcgtactcga cgacgaaccc
     1981 gagcgcggcc agcaccaccg agagcaccgc ggcgatcccg gaggcggcca cgaccgatcc
     2041 acggttggcc ggcaacaccc cgcggatcaa ccggaagatc gcatacccgc cgaacgacgt
     2101 gatcagggcc atgttcaaca cgttgagccc gagcgcggtg agcccgccgt cggcgaacaa
     2161 cagcgcctgc accaccagca ccaccgccac cgcgaccgcc ccagcatagg gtccgaccag
     2221 gatcgcggcg aggcacccac ccagcaggtg cccgctggtt cccgcggcga cagggaagtt
     2281 caacatctgc accgcgaaga tgaaggcggc caccagaccc gccatgggcg cgagcttctc
     2341 cgacagggtc tccttggcct tgacgaggct gtagccgatg ccaccggccg agaccacgcc
     2401 cgcggccgcc gatacgcccg cgttgatgaa cccgtcaggg atgtgcatcc gtgcctcccg
     2461 tagaccgacg ctcgtcgccg gagcagctcg ttgcagttgc aactcgttgc gattccgacg
     2521 actcctgtat atcgccgacc tcctgtgagc gtcaagcgca cgatctcggc agccggtcgc
     2581 tcagaagcgc ggggtctccc ggtagtcgcc gaacaccccg acgagggcgc tcacgatctc
     2641 gccttccgtg caccgggttc gcgcacactc gaccatcgga tcgatcaggt tctcgtcggt
     2701 gccggccgcg cggacgaggt cggcgagcgc ggccttcgcg acggcatcgt cgcgctcggc
     2761 tcgggtccgc tcgacggcca cgcgttgctc gacctcggcc tccgggccga tcacgagggt
     2821 gtcgatcggc gcctcatcgg tctcgacgaa ggcgttgacg cccaccttca ccaggtcgcc
     2881 ggtctcgtag cgacgctgct cctcgaacgc ggcgtcggcg atgcgctgct ggaaccagcc
     2941 ctgttcgatc ccggccagga cgccgtcgag catcgagccc tcccccatcg catcgatgtg
     3001 ggcgaagatc tcctcggcct gggcctcgat ccggtcggtc atcgtctcga cgaagtagga
     3061 cccgccgagc gggtcgatcg tgtcgggcgc cccggtctcg taggcgatca cctgctgcgt
     3121 gcgcagcgcg agcttggcgg cgtcctcggt cggcagggcc agcacctcgt cgagcgcgtt
     3181 cgtgtggagc gactgcgtcc ccccgaacac cgcggcgagc gcctcgacgg cggtccgcac
     3241 cacgttgttc atcggttgtt gcgcggtgtt ggagaccccg gcggtctgcg tgtggaaccg
     3301 cagacgcatc gcggcgggat cggtggcgcc gtatcgatcg cgcagccatc gcgcccagat
     3361 gcgccgagcg gcgcggaact tcgcgatctc ttcgaagaag tcgatgtggc tgttgaagaa
     3421 gaacgacagc ccggaggaga agcgatccac gtcgagcccg cggcgctggc cgagttcgac
     3481 gtagccgaac ccgtcggcca gcgtgaacgc cagctcctgg gccgccgtcg ctccggcctc
     3541 gcggatgtgg tagccgctca cgctcaccgg atggaacttg gggcaccgct cggcacagaa
     3601 ggccatgagg tcgccgatca gccgcagatg cggacgcggc gggaagatcc attccttctg
     3661 cgcgatgtac tccttgagga tgtcggtctg cagcgtgccg tcgagctgat cccacgccac
     3721 cccttggcgt tccgccgtgg ccaggaagaa cgcgaacgcc acggtggccg gtccgctgat
     3781 cgtcatcgac gtgctgatgt cgcccaaggg gatcgagtcg aacagcgtct cgaaatcatc
     3841 gagggaatcg gtcgccacac cgcagcgccc cacctcgccc tcgctgcgag ggtcgtcgga
     3901 gtccagcccc atcagggtgg gcatgtcgaa ggcgacgctg agcccaccct gaccctgcgc
     3961 cagcaggaag cggtaccgcg cgttggtctc ggcgggcgtg ccgaatccgg cgaactgccg
     4021 catggtccac aggcgacccc ggtacatcga ggggtagacc ccgcgcgtga acgggaactc
     4081 ccccggccgg cccagtcgtt cgtcggggtc ccaggaggcc aggtcctcgg ccgtggcgat
     4141 ctcgggaacg ggttcgcccg acagcgttcg atgcgaacgc tcagcggcaa cggcctcgtc
     4201 ggtgggttgg gtgcgttggc gggcgtcggt cactgtggct cgcgtccctg cgatgtggtg
     4261 gcggtcggcc gcggcctact ccgcggcccg ggaggccagc atagcggcag cgcggtaggg
     4321 atcgagacgg cgggcctcga gctcgtcggc cagctgcggg tctgcgcgaa gggcctcggc
     4381 ggcgcgaagc cggaaccgct cggcggccag gctctccacc tcgcggatca ggcgggaccg
     4441 ccgcttggcc gcgagagcac cggacgctgc caggtggtcg cgatgcgatt cgatggcctc
     4501 ccacagctcc tcgatgccct cgccctggga tgccgatgcg gtgaggaccg gggggtccca
     4561 gtcgcgggcc gcaccgaggt gcagcatctg gcggagttcg cggacgacct ccggggcccc
     4621 gtcgcgatcg gccttgttga cgacgaagat gtcggcgacc tcgaggatcc cggccttcgc
     4681 catctgtacg gcgtcgccga ggccgggcgc cagcaccacc accgcggtgt cggtggcggc
     4741 cgccacatcg acctcggcct ggcccacccc cacggtctcg accacgacgc gttcgtaccc
     4801 cgccgcgtcc aggatgcgga ccgcttcagg agccgcgagc gccatgccac caagatgtcc
     4861 ccgggtggcc atcgaccgga tgaacacgcc ggggtcggtg gcgtgctcct gcatgcggac
     4921 gcgatcgccc agcagcgcgc cgccggtgta gggggaggtc ggatcgaccg ccagcacggc
     4981 gacggggagg tcgcgggtcc gcgccgcacc caccaatccg gccgcgagcg tcgacttacc
     5041 cacgcccggc gccccggtca ggccgatcgt cgcggccgag cccgtgtgtg gatacaggcc
     5101 cgcggacagc gactccaggt cggcgtcgcc gtcctcgacg gccgagatcg cgcgggccac
     5161 cgcacggcgt tcgcgcgcca gcaccccacg gacgagctcg tcgacctcca tcgatcgcag
     5221 cctacccgcc gccgctcgcc gtctgggcgg gcgcggcgcc ggggatcgcc tggagggcat
     5281 cgcgcagcga acggcagtac tcccgttgcc gcatcagggc gggatcctga tcatcgggca
     5341 ccctggacgc caccatggcg aggttcacct gttcgcgttg gagtcgatgc agcagcgagg
     5401 acgcccgagg ccccgcgcgc cggcgcatct gcgcacgggc ggcgcgacgg cgcttgggcg
     5461 aggcgagcac ctggagctcc tcggcggaga tgccatcgag gccgacctcc ggcgagaggg
     5521 aagcctgcaa ccagcgccgt tcccgacgcc gggccagcca gaccgcgata cccacgaacg
     5581 ccagcaacgg cagcccttt
//
LOCUS       DATDBZ010000274        10005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1526687, whole genome shotgun sequence.
ACCESSION   DATDBZ010000274 DATDBZ010000000
VERSION     DATDBZ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526687"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VIX62_06705"
     CDS             <1..195
                     /locus_tag="VIX62_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEY5859956.1"
                     /translation="LVKEGREHGQAVGYLEDGTMVVVEKAVEEIGSSADIKVTNVIQT
                     TTGRMVFGVLAPDGESVPAS"
     gene            258..956
                     /gene="ispD"
                     /locus_tag="VIX62_06710"
     CDS             258..956
                     /gene="ispD"
                     /locus_tag="VIX62_06710"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY5859957.1"
                     /translation="MDPEPLGAVAIVLAAGAGRRLGAVDQPKAFLPIGGRPMLTVAAT
                     AAAASESVRSLVVVAPAGWEDYAQGCVEFCGRPAVVVTGGDSRQASVRAALAVIDDAP
                     VVAVHDAARPFAAPDLFTAVIDAIDDATIGVIPVVPVTDTVKRVLDGVVVGTVDREEL
                     ALAQTPQAFATEALRAAHDRAVEAGLTLTDDAAVLELAGHQVRAVPGDPMNSKITTLF
                     DLAQAEARMGGGDG"
     gene            949..1440
                     /gene="ispF"
                     /locus_tag="VIX62_06715"
     CDS             949..1440
                     /gene="ispF"
                     /locus_tag="VIX62_06715"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY5859958.1"
                     /translation="MAETPRVGIGFDVHPRAEGRPLMLGGIRFEGVDGLRGHSDGDAV
                     CHALADALLGAAALGDVGEHFPESDPALEGIAGLDLLARTLTLVADAGFAAASCDLTV
                     IGERPSIAPRRSEMRSALAGALGLPVGAVSVKATRPEGLGLQGDGVGCMAIALLAPIA
                     GSR"
     gene            1437..2258
                     /gene="rlmB"
                     /locus_tag="VIX62_06720"
     CDS             1437..2258
                     /gene="rlmB"
                     /locus_tag="VIX62_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY5859959.1"
                     /translation="MSRDRDRAHRRRTRPPEPGSVVGGRRAVTEAIRAGRATEVLVVS
                     SPKVTQGMRSLLEAAERAGVRITVTPRARLDGMAADHQGVVARIDAPDREEGSGVLTE
                     REVANYPFAEDAIVVVLDGITDPHNLGAAARSAEAAGAAMLITRTKRAADVTASAVRA
                     SAGALLHLPHARVANIARALDRLQDAGFWVLGLDGDAAASIYDEPAPEGKVALVVGSE
                     GEGMSRLVRERCDILVRLPMHGRVGSLNASASLAAVLYGGVLPAREATVPAADGR"
     gene            2297..2752
                     /locus_tag="VIX62_06725"
     CDS             2297..2752
                     /locus_tag="VIX62_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859960.1"
                     /translation="MDESLPPPPHAGYEAWADRVPPGTDPNVAPRRPGVITAAGVLLL
                     VSAGFSLLGALILLSSGARPTLTGLGSARAFAAVLVIVGGLHLLAGILVLARMNGGRI
                     LAIVLASLGVLGALSQLRSATGFVSLAINGFVVYALAANGDAFRRRTRR"
     gene            2768..2841
                     /locus_tag="VIX62_06730"
     tRNA            2768..2841
                     /locus_tag="VIX62_06730"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2802..2804,aa:Thr,seq:tgt)
     gene            3127..3618
                     /locus_tag="VIX62_06735"
     CDS             3127..3618
                     /locus_tag="VIX62_06735"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY5859961.1"
                     /translation="MSVPWDDRSVDVKRSGRTRGARRHEEAGAAAVEFAIVASLFFML
                     VFAIIDFGFGFHTWNGTAHAAREGARLAAVDPDPAAIEGRVREAAGFLDQTKLTVSVQ
                     CSRGGGGFSACPTSASSWAEGDYVRVIVDYQYPYFTPLPGFVGLGDDLNLHSQSEVRF
                     EGA"
     gene            3621..5000
                     /locus_tag="VIX62_06740"
     CDS             3621..5000
                     /locus_tag="VIX62_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY5859962.1"
                     /translation="MNKILRRVHARANDESGAIIIMAMFLMVVLFGAVAFTIDLSRLF
                     HERQVLQNAVDFGALAGAQELPAQGSTQGAIASAKALQVTLDNAPWIDPATISITFKC
                     VVGDRNGDGAPDLSDVPYICGPSTGTWLTDWKTKRGRSQHTCNPYIGDKCNTVSVRTS
                     ETVNYIFAPVIGINQGSTGSVSAASCRGACGAQPNPLDVVMVIDRTGSMTTADLSNAK
                     NAARSVLDFYDSSEHHVGVVALPYGRPTDKCNANDPQLYPQAAASYWSIAPLSSDYDR
                     SDGSLNTSSQIVTAINCLKVSPTIVVRVNGVVKTNAGHTNLGDPLDAAREMLLTQGRS
                     DVPDIIIFMTDGEANQPDGLQPCSYLNNNANIAKAAGQTIYTIAYGVGGARCSRDTSG
                     AFRNAYASTNLAKAATNSTDDVPGGCGINENKDGDNYFCESGSGDLEPVFRQVAAATL
                     GHSRLIEDL"
     gene            5032..5754
                     /gene="cpaB"
                     /locus_tag="VIX62_06745"
     CDS             5032..5754
                     /gene="cpaB"
                     /locus_tag="VIX62_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY5859963.1"
                     /translation="MRRQTLVIPIALLLAVMAAAGVVLYTMNVKEEATTGGPTSTVVV
                     ASEDVPAGTELDPLIEEGKLVEQEIPKNYIVPGAVGSLEELQGRTVSQPLVEGEQILA
                     NRIDELGTVEGGSLGIPDGFTAYTVELRPEQTVANVLNRGDRVRVFATFSEKSGARAS
                     VALLPDVKVLAVSSDGDPEQQAASNQLVTLAVTPDDAAKLALFGQDTGSLWLNLLPPD
                     APKDQVPDPFTDVDLIALAKKS"
     gene            complement(5767..6972)
                     /locus_tag="VIX62_06750"
     CDS             complement(5767..6972)
                     /locus_tag="VIX62_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5859964.1"
                     /translation="MDVGFHPADPGFVADPYPVYRELRDRSPVTHDEALDLWLVARYE
                     DVNALLRDRRLGRTYLHVASHEEMGHTAPPAQHDPFWHLITNGILDMEPPDHTRVRRL
                     VAKAFSPRMVESMRAPVQGLMDGLIEGRRGAGSFDLIAAVAEPLPVAIIAELLGIPAA
                     DRHRLRPWSADICRMYELQPTDEDAAIAVRASIEFSDYLRELSRERRASPAGDLISEM
                     TSVVDDGDHLTEDELIGTCVLLLNAGHEATVNVTGNGWWSLFRHPDALARLRAQPALV
                     PSAVEEIMRWDTPLQMFERWALEDVEIGGITIPRGSEFGLLFGSANRDPEVFTDPDAF
                     DLEREPNPHLTFGAGIHFCLGAPLARLELQTSFSTVLRGLPDLVLVEEPSWKPNYIIR
                     GLDRLLVSA"
     gene            6995..7519
                     /locus_tag="VIX62_06755"
     CDS             6995..7519
                     /locus_tag="VIX62_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY5859965.1"
                     /translation="MRVVRWVGIAGLVTMTACGALGGSGERVTDRPVPGAGGPRSDAT
                     VTRIVDGDTIHVDLDGRDTTIRIIGIDTPEKDGPYTDEECFGQEATRYTEQALAGREV
                     ELEFDVDRTDRYDRTLAYVWVGNELFDERILEDGYAVLLTVPPNVRYVDRLTQAQRAG
                     REAGAGLWGACPGG"
     gene            complement(7516..9096)
                     /locus_tag="VIX62_06760"
     CDS             complement(7516..9096)
                     /locus_tag="VIX62_06760"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY5859966.1"
                     /translation="MPAHLDILIESVTLIDGTGAPGFTATVGVAAGSVAWVSRTRPED
                     APAARTIDGEGLVLAPGFIDVHNHSDLGALVDPEMPSTLRQGVTTVVVGNCGSSPWPV
                     AGAVECAQLVGGDPATMDLAFPSFAGYLDRLDAARPAVNVAALVGHGAIRSQVMGGQR
                     RPPTGEELGGMRRAVSSAMDDGALGLSTGLIYAPGMYARTDEVVALAEETAGRGGIYA
                     SHIRGEGEHLFRAVDEAIEIGRRAGGPAHVSHLKCETSLVWGRAGELLQRFHGDDDVT
                     GDQYPYEAWASVLWSLLPEWAPVDEVARIWADPDRRTRLAQAIEEGEGDAFQSSVRGV
                     GWDRIVIEATADTACNGESIAAIAQERGVAPVEACIQLLIEEPQTACIGHAMAEDDVR
                     TIMADPDVMVASDASSMSPTGPLGGLPVHPRNYGTFPRVLGRSVREGVLTLEAAVRKM
                     TSLPADRFGLPKRGRIEQGAVADLVLFDPVTVQDRADFDAPHTFPEGIRAVIVDGTIA
                     WSADTGAIERSGRVLRRT"
     gene            complement(9106..>10005)
                     /locus_tag="VIX62_06765"
     CDS             complement(9106..>10005)
                     /locus_tag="VIX62_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5859967.1"
                     /translation="QDHTVEYRVITSQGSTLWLRDLVHVVRGAQGPKQLRGLMVDVTD
                     RKRAEQALRKSERKYSEAFRREREAAQRLRALDDMKNTFLEAVSHDLRTPLTSILGSA
                     LTLEQTRFELPKAESLDLVRRVASNARKLERLLSDLLDLDRLQRGIVSPQRRPTDLAA
                     LVARTVDELEDPAGHEIDVDVEAVTVSVDAAKVERIIENLLSNAIRHTPPATRIWLKA
                     SSLDGGLMLVVEDEGPGVPEDLREAVFEPFRQAPGSSSEHSPGVGVGLSLVRRFAELH
                     GGRAWLEARDGGGASFKVFLPGG"
BASE COUNT         1570 a         3369 c         3529 g         1537 t
ORIGIN      
        1 ctggtgaagg aggggcgcga acacggtcag gcggtgggct acctcgagga cgggacgatg
       61 gtcgtcgtgg agaaggccgt cgaagagatc gggtccagcg ccgacatcaa ggtcacgaac
      121 gtgatccaga cgaccaccgg ccgcatggtc ttcggcgtgt tggcccccga cggcgagtcc
      181 gtgccggcct cctagctgcc gcctcttgat cggttggcca ccggctggca cccggacgtg
      241 tctggcatgc ttcgggcatg gatccagagc ccctcggcgc cgtcgcgatc gtcctggccg
      301 ccggcgcggg cagacgtctg ggcgcggtcg accaaccgaa agcgttcctg cccatcgggg
      361 gcaggcccat gctgacggtg gcggccaccg ccgctgctgc ctcggaatcg gtccgttcgt
      421 tggtcgtggt cgcgcccgca ggctgggagg actacgcgca ggggtgtgtg gagttctgcg
      481 gacggccggc ggtggtggtg accggcgggg actcgcgaca agcatcggtg cgtgccgcgc
      541 tcgccgtgat cgatgatgct ccggtcgtcg cggtgcacga tgcggccagg ccgttcgctg
      601 cgcccgatct gttcaccgcc gtgatcgatg cgatcgacga cgcgacgatc ggcgtcatcc
      661 ccgtcgtccc ggtcacggac acggtcaagc gggtgctcga cggggtggtc gtcggcacgg
      721 tcgaccggga ggaactcgcg ctggcacaga cgccacaggc gttcgcgacc gaggccctcc
      781 gcgccgctca cgaccgcgcc gtggaggcag ggctcacgct gaccgacgac gccgcggtgc
      841 tggagttggc cggtcatcag gtccgcgccg tgcccggtga cccgatgaac tcgaagatca
      901 ccaccctctt cgatctcgca caggccgaag cccggatggg aggcggggat ggctgagacg
      961 cctcgcgtgg ggatcgggtt cgacgtccat ccgcgggcgg aaggacgacc gttgatgctc
     1021 ggtggcatcc ggttcgaggg tgtcgacggc ctcagggggc attccgacgg cgacgcggtg
     1081 tgtcacgcgc tggccgacgc cctgctggga gcggccgcgc tcggtgatgt cggggagcac
     1141 ttccccgaat cggatcccgc cctggagggg atcgccgggc tcgacctcct cgcacgcacg
     1201 ctcaccctcg tcgcggacgc ggggttcgcg gccgcgtcct gcgatctcac ggtgatcggc
     1261 gagcggccct cgatcgcgcc gaggcgatcc gagatgcgct cggccctcgc cggggcgctc
     1321 ggtcttccgg tcggcgcggt ctcggtcaag gccacgcgtc ccgaaggact cgggttgcag
     1381 ggcgacggcg tcggctgcat ggcgatcgcc ctgctcgcac cgatcgcggg ttcgcgatga
     1441 gccgcgaccg cgaccgagcc catcgacgcc ggacgcgacc gccggagccc gggtcggtcg
     1501 tgggtgggcg gcgcgcggtc acggaggcga tccgtgcggg gcgggccacc gaggtcctgg
     1561 tggtgtcctc accgaaggtg acccagggga tgcgctcgct cctcgaggcg gcggaacgcg
     1621 ccggggtgcg gatcacggtg accccgcgcg ccaggctcga cggcatggcc gccgaccacc
     1681 agggcgtggt ggcccggatc gatgcccccg accgggagga gggctcgggc gtcctcaccg
     1741 agcgagaggt cgcgaactat ccgttcgccg aggacgccat cgtcgtggtg ctggacggca
     1801 tcaccgaccc ccataacctg ggtgcggctg cgcgttcggc cgaagcggcg ggtgcggcca
     1861 tgctgatcac caggaccaag cgtgccgccg atgtcaccgc gagcgccgtt cgagcctcgg
     1921 ccggggcgct gctgcatctg ccgcatgccc gtgtcgcgaa catcgctcgc gccctcgacc
     1981 ggctgcagga tgccgggttc tgggtcctgg ggctggacgg cgacgccgcg gcgtcgatct
     2041 acgacgagcc cgcacccgag ggcaaagtgg cgctcgtcgt gggcagcgag ggggaaggga
     2101 tgtcgaggtt ggtgcgtgag cggtgcgaca tcctggttcg actcccgatg cacgggcgcg
     2161 tgggctcgtt gaacgcgtcc gcgtcgctgg ccgcggtcct gtacggcggc gtcttgcccg
     2221 cgagagaggc tacggttccg gccgccgacg gccgatgacc tgaccggagg atccgcaccg
     2281 cgcgggtcga acggtcatgg acgagtcgct gccgccaccg ccccacgccg gctacgaagc
     2341 gtgggccgat cgcgttcccc cgggaaccga tccgaacgtc gcccctcgac gtccgggggt
     2401 gatcacggcc gcgggggtcc tgctcctggt gtccgccggc ttctcgttgc tgggcgcgct
     2461 gatcctgctg agctcgggag ccaggcccac gttgaccggg ctggggtcgg ctcgggcctt
     2521 cgccgccgtc ctcgtgatcg tcggaggctt gcacctgctg gccggcatcc tcgtcctggc
     2581 ccggatgaac ggcgggcgca tcctcgcgat cgtgctcgcc tcgctcgggg tgctgggcgc
     2641 gttgtcccag ctccgttcgg cgacggggtt cgtgtcgctc gccatcaacg gattcgtcgt
     2701 gtacgcgctc gccgcgaacg gggatgcgtt caggcggcgg acacggcgat agactcgctc
     2761 ccgcccggcc ggcgtggcgc agcccggtag cgcaagcgat ttgtaatcgc taggtcgtcg
     2821 gttcgaatcc gaccgccggc tctgacgttc gcccccccac catccgacct acgctcgaag
     2881 cctccttcgg tcctatacgg cgcagggcac cgatgtgccg atggaacggg catatccacg
     2941 agggcctggc gatggatcga gggggatgac atgccggctc aagcctgccg ccgaggatcg
     3001 accgcggtat ccgccgctgg ccgatcgcgc tcggctccgc ctttcgtctc tcgcccgcgc
     3061 gcgggcagcc gtcgaccatc ggccgtggct gcgctcaagg agaggcctca aactttcgat
     3121 aaggaaatgt ccgtcccatg ggatgacagg agcgtcgatg tgaagcgatc cgggcgaacg
     3181 cgcggagcaa ggcgacacga agaagcagga gcggcagcgg tcgaattcgc gatcgtggcg
     3241 tcgctgttct tcatgctggt cttcgccatc atcgatttcg gtttcgggtt ccacacgtgg
     3301 aacggcaccg cgcatgcggc gcgcgaaggc gctcgtctcg cggccgtcga tccggatcct
     3361 gcggcgatcg agggacgcgt ccgcgaagca gccgggttcc tggaccagac caaactcacc
     3421 gtctccgtgc aatgcagcag gggtggcggc ggcttctccg catgccccac ctcggcctcc
     3481 tcgtgggccg agggcgatta cgtgcgggtc atcgtggact accagtatcc gtacttcaca
     3541 ccactgcccg gtttcgtcgg actcggggat gacttgaacc tgcattcgca gtctgaggtc
     3601 cgattcgagg gggcatagcc gtgaacaaga tcctgcgccg ggtgcacgcc cgggccaacg
     3661 acgagtccgg agccatcatc atcatggcga tgttcctgat ggtggtgctg ttcggtgcgg
     3721 tcgcgttcac gatcgacctg tcccggctct tccacgaacg tcaggtgctg cagaacgcgg
     3781 tggacttcgg cgcgctcgcc ggtgcacagg agctgccggc gcagggttcg acccaaggag
     3841 cgatcgcgtc ggcgaaggcc ctgcaggtga cgctggacaa cgctccatgg atcgatccag
     3901 ccaccatcag catcaccttc aagtgcgtgg tgggagaccg caacggcgac ggggcgccgg
     3961 acctgtcgga cgtgccgtac atctgcggac cgtcgacggg aacgtggttg accgattgga
     4021 agaccaagcg cggccggtca cagcacacgt gcaacccgta catcggggac aagtgcaaca
     4081 cggtcagcgt gcgcacgagt gagaccgtca actacatctt cgcgcccgtg atcgggatca
     4141 atcaggggag caccgggtcg gtgagcgcgg cctcgtgccg aggcgcgtgc ggcgctcaac
     4201 ccaatccgct cgacgtggtg atggtgatcg accgaaccgg ctcgatgacg accgccgacc
     4261 tttcgaacgc gaagaacgct gcccggtccg tgctggactt ctacgactcg tccgagcacc
     4321 acgtcggcgt cgtggccctg ccgtacggga gacccacgga caagtgcaac gcgaacgatc
     4381 ctcagctcta cccgcaggct gcggcgtcgt actggtcgat cgcgccgctg agctcggact
     4441 acgaccgatc ggacggatcg ctcaacacgt cgagccagat cgtcacggcc atcaactgcc
     4501 tcaaggtctc gcccacgatc gtcgtgcgcg tgaacggcgt ggtcaagacg aacgccggcc
     4561 acacgaacct cggcgatccg ctggacgccg cccgggagat gctgctcacc cagggtcgat
     4621 cggacgtgcc cgacatcatc atcttcatga ccgacggtga ggcgaaccaa cccgacggcc
     4681 tgcagccgtg cagctacctg aacaacaacg cgaacatcgc caaggcggcc ggtcagacga
     4741 tctacacgat cgcctacggg gtcgggggcg cgcggtgctc gcgtgacact agcggggcct
     4801 tccgcaacgc gtacgccagc acgaacctcg ccaaggccgc caccaattcc accgacgacg
     4861 tccccggtgg atgcgggatc aacgagaaca aggacgggga caactacttc tgcgagtcgg
     4921 ggagcggaga cctggagccg gtgttccggc aagtggccgc cgcgaccctc ggacactcgc
     4981 ggttgatcga ggacctctga cggagcggtc tggacacagg aggagacccg aatgaggaga
     5041 caaacactgg tgatcccgat cgccctgctg ttggccgtga tggccgcggc aggcgtggtc
     5101 ctgtacacca tgaacgtgaa ggaggaggcg accaccggcg gaccgacgtc caccgtggtc
     5161 gtcgcatccg aggacgtgcc ggcaggcacc gagctcgacc cgctgatcga agaaggcaag
     5221 ctggtcgagc aggagatccc caagaactac atcgtgcccg gcgccgtggg ttcgctggag
     5281 gaactccaag gccgcacagt gagccaaccg ctggtcgaag gtgagcagat cctggcgaac
     5341 cggatcgacg agctcggcac ggtcgaaggt gggtcgctgg ggatccccga tggcttcacc
     5401 gcctacacgg tggagctgcg tcctgagcag accgtggcca acgtcctgaa ccgcggggac
     5461 cgcgtccggg tgttcgcgac cttctccgag aagagcggcg ctcgtgcatc cgtggcgttg
     5521 ttgcccgacg tcaaggtctt ggccgtctcc agcgatggtg atccggaaca gcaggcggcg
     5581 agcaaccagc tcgtcacgct ggcggtcacg cccgatgacg cggcgaagct cgcgttgttc
     5641 ggacaggaca ccggttcgct gtggctgaac ctgttgccgc cggacgctcc caaggaccag
     5701 gtccccgatc cgttcacgga cgtcgacctc atcgccttgg ccaagaagag ctagacgggg
     5761 aaggcttcag gcgctcacca ggagccggtc cagaccgcgg atgatgtagt tcggcttcca
     5821 cgagggctcc tcgaccaaca ccaggtcggg gagccctcgg agcaccgtgg agaacgacgt
     5881 ctggagctcg agcctggcca gcggggctcc caggcagaag tggatccccg cgccgaaggt
     5941 caggtgcggg ttcggttcgc gctcgagatc gaaggcgtcc ggatccgtga agacctcggg
     6001 atcgcggttc gccgatccga acagcaaccc gaactccgag cctcgcggga tcgtgatgcc
     6061 accgatctcg acgtcctcca gcgcccagcg ctcgaacatc tgcagcgggg tgtcccaccg
     6121 catgatctcc tcgaccgctg agggaacgag cgcgggttgg gcacggagcc gagcgagcgc
     6181 gtcggggtga cggaacagcg accaccatcc gttgccggtc acgttcacgg tggcttcgtg
     6241 gccggcgttg agcagcagga cgcacgtgcc gatcagctcg tcctcggtga ggtgatcacc
     6301 gtcgtcgacc accgaggtca tctcgctgat caggtcgccc gcgggcgaag cacgtcgctc
     6361 cctcgagagc tcccggaggt agtcggagaa ctcgatgctg gcgcgcacgg cgatcgcggc
     6421 atcctcgtcg gtcggctgga gttcgtacat gcggcagatg tcggccgacc acggtcgcag
     6481 gcgatgccgg tcggccgcgg ggatgccgag cagctcggcg atgatggcga ccggaagcgg
     6541 ctcggccacg gcggcgatca ggtcgaagct cccggcccct cgccgtccct cgatgagccc
     6601 gtccatcagg ccctgcaccg gcgctcgcat cgactcgacc atgcgcggcg agaaggcctt
     6661 ggccaccagc cggcgaaccc gcgtgtggtc gggcggttcc atgtcgagga tgccgttcgt
     6721 gatgaggtgc cagaacggat cgtgttgcgc cggcggggcc gtgtgtccca tctcctcgtg
     6781 cgaggcgaca tggaggtagg tgcgacccaa ccggcgatcc cgtaggaggg cgttcacgtc
     6841 ctcgtaccgg gccaccagcc acagatcgag ggcttcgtcg tgggtcaccg gggagcgatc
     6901 gcggagctcg cgatagacgg gatacgggtc ggcgacgaag ccgggatcgg cgggatggaa
     6961 gcccacgtcc atccacctac ggtagcgtcc ggccatgcgt gtggtccgat gggtcggcat
     7021 cgccggactc gtgacgatga ccgcctgcgg agcgttggga ggctccggtg agcgggtgac
     7081 ggatcgtccg gttccggggg ccggagggcc gcgctcggac gcgacggtga cccggatcgt
     7141 cgacggcgac acgatccacg tcgacctcga cggacgggac accacgatcc ggatcatcgg
     7201 gatcgacacg cccgagaagg acgggcccta caccgacgag gagtgcttcg ggcaggaggc
     7261 cacccgctac accgagcagg cgctcgccgg gcgcgaggtc gagctggagt tcgacgtcga
     7321 tcggaccgat cggtacgacc gaaccctggc ctacgtctgg gtcgggaacg aactcttcga
     7381 cgagcgcatc ctcgaggacg gctacgccgt gctcctcacc gtcccgccga acgtgcgata
     7441 cgtcgaccgg ctcacgcagg cacagcgggc ggggcgggag gccggcgccg ggctgtgggg
     7501 agcctgcccc ggcggctaag tccgacggag cacgcgcccc gaccgttcga tcgcccctgt
     7561 atccgccgac cacgcgatcg ttccatcgac gatgaccgcc ctgatcccct ccgggaaggt
     7621 gtgcggagca tcgaagtcgg cccggtcctg cacggtgacg gggtcgaaca gcaccaggtc
     7681 ggcgaccgcg ccctgctcga tgcggccgcg cttcggcaac ccgaaacgat cggcaggcag
     7741 cgaggtcatc ttgcggacgg cggcctccag ggtcagcaca ccctcacgta cggagcgccc
     7801 aaggacccgg gggaaggttc cgtagttgcg agggtggacg gggagcccgc cgagcggccc
     7861 cgtcggcgac atcgacgacg cgtcggaagc caccatgacg tcggggtcgg ccatgatcgt
     7921 gcggacgtcg tcctcggcca tcgcgtggcc gatgcacgcc gtctgcggtt cctcgatcaa
     7981 caactggatg caggcttcga ccggcgccac cccgcgttcc tgcgcgatcg ctgcgatgct
     8041 ctcgccgttg caggccgtgt cggcggtggc ctcgatcacg atccggtccc atcccacgcc
     8101 gcggaccgag gactggaacg cgtcgccttc gccctcctcg atcgcctgtg ccagcctcgt
     8161 gcgacggtcg gggtcggccc agatccgggc gacctcgtcc acgggggccc actcgggcag
     8221 tagcgaccac aggaccgagg cccacgcctc gtaggggtac tggtcgccgg tgacgtcgtc
     8281 gtcaccgtgg aaccgctgca ggagttcacc ggccctgccc cagaccaacg atgtctcgca
     8341 cttgagatgg ctcacgtgcg cggggcctcc cgcgcgccgg ccgatctcga tcgcttcgtc
     8401 gaccgcgcgg aacagatgct caccctctcc gcggatgtgt gacgcgtaga tgccgcctcg
     8461 gccggcggtc tcttccgcga gggccacgac ctcgtccgtg cgcgcgtaca tgcccggcgc
     8521 gtagatcagc cctgtggaga ggccgagggc gccgtcgtcc atcgcgctcg acacggctcg
     8581 acgcatcccc ccgagttcct ccccggtcgg tgggcggcgt tgcccgccca tgacctgcga
     8641 gcggatcgcc ccgtgaccga cgagggcggc gacgttgacg gcgggccgtg cggcgtcgag
     8701 acgatcgagg tagccggcga acgacgggaa cgcgagatcc atcgtcgcgg ggtcgccacc
     8761 caccagctgg gcgcattcga ccgctccggc gaccggccac ggtgaggagc cgcagttgcc
     8821 caccaccacc gtggtcacgc cctgtcgcag ggtggacggc atctcgggat cgaccaaggc
     8881 cccgagatcc gagtggttgt gcacgtcgat gaacccgggc gccaagacga gcccttcacc
     8941 gtcgatcgtt cgtgcggcag gagcgtcctc gggacgtgtc cgcgagaccc atgcgacgga
     9001 ccccgccgcg acacctaccg tcgcggtgaa cccgggcgca cccgtcccat cgatcagcgt
     9061 gacggactcg atgaggatgt cgaggtgcgc gggcatcgcc cgatgctagc cgccaggcag
     9121 gaacaccttg aacgacgcgc ctccgccgtc ccgggcctcg agccacgcgc gacccccgtg
     9181 cagttcggcg aaccggcgaa ccagggacag gcccacgccc acccccggcg agtgttccga
     9241 cgaagagccg ggcgcttggc ggaacggctc gaagaccgcc tcccgaaggt cctcgggaac
     9301 gccggggccc tcgtcttcga cgacgagcat cagcccgccg tccaggctcg aagccttgag
     9361 ccagatcctc gtggcgggcg gcgtatgacg gatcgcgttc gacagcaggt tctcgatgat
     9421 gcgttcgacc ttggcggcgt cgacggacac ggtgaccgcc tcgacatcca cgtcgatctc
     9481 gtggcctgcg ggatcttcga gttcgtcgac ggtgcgggcc accagggccg ccagatcggt
     9541 cgggcgtcgc tgtggcgaca cgatgccgcg ctggagtcga tcgaggtcga gcaggtcgga
     9601 cagcaggcgt tcgagcttcc tggcgttgga ggccacgcgc cggaccaggt cgagcgattc
     9661 ggccttgggc agttcgaacc gggtctgctc cagggtcagg gcagacccca ggatcgaggt
     9721 gagcggggtc ctgaggtcgt gcgagaccgc ttccaagaac gtgttcttca tgtcatcgag
     9781 cgcgcggagg cgttgggccg cctcccgttc tcgccggaac gcctcggagt acttgcgctc
     9841 gctcttgcgc agcgcctgct ccgcccgctt gcggtcggtg acgtccacca tgagcccgcg
     9901 cagttgcttg gggccctggg cgccgcggac gacgtgcacg aggtcgcgga gccacagcgt
     9961 gctgccctgg gaggtgatca cccggtactc caccgtgtgg tcctg
//
LOCUS       DATDBZ010000275         3060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2322260, whole genome shotgun sequence.
ACCESSION   DATDBZ010000275 DATDBZ010000000
VERSION     DATDBZ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322260"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VIX62_06770"
     CDS             complement(<1..468)
                     /locus_tag="VIX62_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5859968.1"
                     /translation="MSPSEFVQQLVNALSLGSIYALLALGLSMVYGVLGMLNFAYGEL
                     VTITGYSMLAATYAGAPFIVVALVGVIAAGISSVLMELIAFRPLRNASFVSLLFTSFA
                     VAQLIQGAFRQTVSVRSKGIPVPAFFDEAVQIGPVRISVLSMITAGIGAISLVA"
     gene            complement(485..1198)
                     /locus_tag="VIX62_06775"
     CDS             complement(485..1198)
                     /locus_tag="VIX62_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5859969.1"
                     /translation="MLAVDGLTVAYGAVVAIRDVSLTVAPGEIVAALGPNGAGKTTLL
                     RTLAGALKPQHGSVTFDGEALTGLSPETVVRRGVALVPEGRHVFGNLTVAENLTIGGI
                     ARTDRDGLRADTERWLARFPILGERANQTAGTLSGGEQQQLAIARALMSRPRMLLLDE
                     PSLGLAPIFVDRIFELIQELRDEGVTVLLVEQNVHRALEIADRAYVLSVGAVVASGPT
                     DRLAEGELERSYLGISQTG"
     gene            complement(1258..2445)
                     /locus_tag="VIX62_06780"
     CDS             complement(1258..2445)
                     /locus_tag="VIX62_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5859970.1"
                     /translation="MLRRSVALAFVLCLVGAACSTSDDANEDGGDGSASGEPIVIGFP
                     ADLSTDWAAFDSPMEEGAQFAVDQINEEGGVLGRPLELKAVDMRNDVAEAAKVTQQLI
                     DDGSVYLIGTVGDGILAEGQVACATGVPISTGLGTAPSLVGDMGDCAYQLVMSDNIQA
                     AVLAEYAIAEGYETAYVLGSNEIPYTRDLPTFFSDAFENGGGTVVATDEYKLGAGDYS
                     AVVTKIQNFDPAPDVIFTPMYIPDSVVFLRQLRQAGVTTPVLSTDGNGDPALLDAGVK
                     AIDGLTFSNSVCTAEGDPNVQQFYDDYNAANGKDPSSYVAVVGYDEVNMVADAIEAAG
                     STEPADITAQLDTIDATGISGHVVMDPETRRADKPAALVQMDGTEFTCLEQPDFPDYV
                     PAI"
     gene            2556..>3060
                     /locus_tag="VIX62_06785"
     CDS             2556..>3060
                     /locus_tag="VIX62_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEY5859971.1"
                     /translation="MREGPTHTSETIGEEIRERLGEMTPSERRVARTLLATYPTAGLE
                     SLPQLAESSGVTGPTVLRFVRKIGFDGYPDFQRSLRQEVQARTEGLPSLYRTKGGSQD
                     DDVLRQSHEAFTSALDATLTSSSLEQDLAPVVALLADPKRRIWFAGGRFSQFVASYLC
                     LQMRMLRP"
BASE COUNT          488 a         1047 c         1048 g          477 t
ORIGIN      
        1 cgcgaccagc gagatcgcgc cgatgcccgc cgtgatcatg ctcagcacgc tgatgcgcac
       61 cgggccgatc tgcaccgctt cgtcgaagaa ggccggcacc gggatgccct tggagcgcac
      121 cgacacggtc tgtcggaacg cgccctggat caactgcgcc acggcgaacg aggtgaacag
      181 gagcgatacg aagctcgcat tccggagcgg gcgaaaggcg atcaactcca tcaggaccga
      241 cgagatcccc gcggcgatca cgccgacgag ggccaccacg atgaacggcg caccggcgta
      301 cgtggcggcg agcatcgagt aaccggtgat cgtgacgagt tcgccgtagg cgaagttcag
      361 catgccgagc acgccgtaga ccatggacaa cccgagcgcc agcagggcgt agatgctgcc
      421 cagggacagg gcgttcacca actgctggac gaactccgat gggctcacgc cgtcacctca
      481 tccttcatcc ggtctgactg atgccgaggt aggagcgctc cagttcgccc tccgccagac
      541 ggtcggtcgg cccggaggcg accaccgcgc ccaccgacag cacgtaggcg cgatcggcga
      601 tctcgagcgc gcggtgcacg ttctgctcga ccagcagcac ggtgacgccc tcgtcgcgca
      661 gttcctggat cagctcgaag atgcggtcca cgaagatcgg cgccaggccc agggagggtt
      721 cgtcgagcag gagcatgcgc ggtcgtgaca tcaacgctcg cgcgatcgcg agctgctgct
      781 gctcgccgcc cgagagcgtg ccggccgtct ggttcgctcg ttcgcccagg atggggaagc
      841 gcgcgagcca ccgttcggta tcggcgcgca ggccgtcgcg atccgtgcgc gcgatgccgc
      901 cgatcgtgag gttctccgcc acggtgaggt tgccgaacac gtgccgcccc tcggggacga
      961 gcgccacgcc gcggcgcacc acggtctccg gcgacaggcc ggtgagcgct tcgccgtcga
     1021 aggtcaccga gccgtgctgc ggtttcaggg cccctgcgag cgttcgcagc agcgtcgtct
     1081 tgcccgcgcc gttgggcccg agcgcggcga cgatctcgcc gggcgccacc gtgagggaca
     1141 cgtcgcggat cgccacgacc gccccgtagg cgaccgtcag cccgtcgacg gcgagcaggg
     1201 gagggccggc gtcggtggcc tcggccgccc cgtcccctcc tcgttcgtcg gtcacgctca
     1261 gatcgccggc acgtagtccg ggaagtcagg ctgctccaga caggtgaact cggtcccgtc
     1321 catctggacc agcgccgcgg gcttgtcggc ccggcgggtt tccggatcca tcacgacatg
     1381 gcccgagatg ccggtggcat cgatcgtgtc cagctgggcg gtgatgtcgg cgggctcggt
     1441 gctgcccgcc gcttcgatcg cgtcggccac catgttgacc tcgtcgtagc cgaccacggc
     1501 cacgtacgag ctcggatcct tgccgttggc cgcgttgtag tcgtcataga actgctggac
     1561 gttcgggtcg ccctcggccg tgcacaccga gttcgagaac gtcagcccgt cgatcgcctt
     1621 gacgccggcg tcgagcaacg ccgggtcgcc gttgccgtcg gtcgagagca ccggggtggt
     1681 gacgcccgcc tgacggagct ggcggaggaa gaccacgctg tcggggatgt acatcggcgt
     1741 gaagatcacg tcgggcgcgg ggtcgaagtt ctggatcttc gtcacgaccg cggagtagtc
     1801 gcccgcgccc agcttgtact catcggtggc caccaccgtg ccgccgccgt tctcgaaggc
     1861 gtcggagaag aacgtcggca ggtcgcgcgt gtaggggatc tcgttcgagc ccaggacgta
     1921 cgcggtctcg tagccctcgg cgatcgcgta ctcggccagc acggcggcct ggatgttgtc
     1981 gctcatcacc aactgatagg cgcaatcacc catgtcgccg accagcgagg gcgccgtgcc
     2041 caggcccgtg gagatcggca cgcccgtggc gcaggccacc tggccctcgg cgaggatgcc
     2101 gtcgcccacg gtgccgatca ggtagacgga gccgtcgtcg atcaactgct gggtgacctt
     2161 ggccgcctcc gcgacgtcgt tgcgcatgtc gaccgccttc agttcgagcg gacggcccag
     2221 cacgcctcct tcttcgttga tctggtcgac ggcgaactgc gcgccctcct ccatggggga
     2281 gtcgaaggca gcccagtccg tcgacaggtc ggccgggaag ccgatcacga tgggttcgcc
     2341 ggacgcggag ccgtcgcccc cgtcctcgtt ggcgtcgtcc gaggtgctgc atgcggcgcc
     2401 caccaggcac agcacgaacg cgagtgcgac cgatctccga agcatccccc aacctcccgt
     2461 cacggtatcg ggcattcgta gacccccctc aggaacctgt caagctcgtt accagctttc
     2521 cctcgtcagc aagatgcgta agaatcctga cttcgatgcg ggaggggccg acgcacacca
     2581 gcgaaacgat cggggaagag atccgcgaac gcctggggga gatgacgccc tccgagcgcc
     2641 gcgtggcccg taccctgctc gccacctatc ccacggcggg attggagagc ctgccgcaac
     2701 tcgccgagag ctcgggggtc accggtccga ccgtgctgcg gttcgtgcgc aagatcggct
     2761 tcgacggcta cccggacttc cagcggtcgc tgcgccagga ggtgcaggcg cgcacggagg
     2821 ggttgccgtc gctgtaccgc accaagggcg gttcgcagga cgatgacgtg ctgcggcaga
     2881 gtcacgaagc gttcacgagc gcgctcgacg ccaccctgac gtcgagttcg ctggaacagg
     2941 acctcgcgcc ggtggtcgcg ctgttggccg atcccaagcg gcggatctgg ttcgcgggcg
     3001 gacggttcag ccagttcgtg gcgagctacc tgtgcctgca gatgcggatg ctgcgaccgg
//
LOCUS       DATDBZ010000276         2625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2943666, whole genome shotgun sequence.
ACCESSION   DATDBZ010000276 DATDBZ010000000
VERSION     DATDBZ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2943666"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VIX62_06790"
     CDS             complement(<1..324)
                     /locus_tag="VIX62_06790"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013262.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY5859972.1"
                     /translation="MTSPVRVAVTGAAGQIGYSLLFRIASGAMLGPDTQVILQLLEIT
                     PSLKALEGVKMELDDCAFPLLAGVVMSDDPNVAFGDVDVALLVGAMPRKAGMERADLL
                     TANGGI"
     gene            complement(364..2379)
                     /locus_tag="VIX62_06795"
     CDS             complement(364..2379)
                     /locus_tag="VIX62_06795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY5859973.1"
                     /translation="MAKAWPPPTAPRKAPISRIPYLPGLDGLRALAVVAVMLYHAGVS
                     WMPGGFLGVEVFFVISGYLITLLLIGEQERRGRLSLSGFWARRARRLLPALIALLVAV
                     ATVSAVLPYLSESLAKLRDELFWGFFYGSNWFQINENLSYFDNQGRPQLLRHLWSLAV
                     EEQFYIVWPLVMVVVLRVFRERLPKVGLAFFVAALASTVWMMVLYDPLNPNRVYLGTD
                     TRAGGLLLGAALATVWRPYAIMRSPLRTKGRILDLVGLAGLLMLALTHWKFHDVVIVD
                     GEIRGYDALYRGGFLLVGLATVMVIACTTHLRSFLGQRVLGIAPLVWLGTRSYGLYLW
                     HWPVFMLLRPGAVDEGGDVDWPGWLVMAVRFAVTFALAEASFRMIERPFRERTVMRWI
                     RSLQKPVPARIAMRRRRVFVGLAVVLLLPVFAGYQVATAKDRPSEVERSLAANEDAVH
                     ELDDILASTTPTTTTTPTVSGSDGDGAAGSNPTEPDVPVVPVESVESVVPEPTTTLPP
                     ETTTTAAPVEPIPMLAIGDSVMLGAASKLAEQGVWVDAKVGRQFKEGVELVTALNAGG
                     LLGDVLVVHLGNNGPSTRERFDELMAQTTNVRLVVVLTVKVPKPWQGEVNTEIFDLPN
                     RYPNVRLIDWNGLSQSQEGIFYSDGIHLRPAGQEVYTQLVMQTIAAG"
     gene            complement(2440..>2625)
                     /locus_tag="VIX62_06800"
     CDS             complement(2440..>2625)
                     /locus_tag="VIX62_06800"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY5859974.1"
                     /translation="VERVGKAGDDVDESARIGIEIATELCAGLLDAGAPGLHFYTLNR
                     SSATRDIYANLGLPIPA"
BASE COUNT          478 a          878 c          896 g          373 t
ORIGIN      
        1 gatcccgccg ttcgcggtga gcaggtcggc gcgctccatt ccggccttgc gcggcatcgc
       61 acccacgagg agcgcgacat ccacatcacc gaacgcaacg ttcgggtcgt cggacatcac
      121 gacgccggcg aggagcggga acgcacagtc gtcgagctcc atcttcacgc cctcgagcgc
      181 cttcagcgac ggcgtgatct cgagcagttg caggatgacc tgcgtgtcgg gaccgagcat
      241 cgcgccggac gcgatgcgga acaggagtga gtaaccgatc tgaccggcag caccggtgac
      301 cgcgacacga acaggtgacg tcatgctccg agcgtaggtc cgtcccacgc ttcgcgggaa
      361 acgtcagcct gcggcgatgg tctgcatcac gagttgtgtg tagacctcct gccctgcagg
      421 gcggaggtgg atgccgtcag agtagaagat gccttcctgc gactggctca acccgttcca
      481 gtcgatcagg cgcacgttgg ggtaccggtt cggcaggtcg aagatctcgg tgttcacctc
      541 gccctgccac ggcttcggca ccttcaccgt gagcacgacc acgaggcgga cgttcgtcgt
      601 ttgcgccatc agttcgtcga agcgctcacg ggtggagggg ccgttgttcc cgaggtggac
      661 gacgaggaca tcgccgagca acccgccggc gttgagcgcg gtgacgagct ccacgccctc
      721 cttgaactgg cggcccacct tcgcatccac ccagacgccc tgctcggcga gcttcgacgc
      781 cgcgccgagc atcaccgagt cgccgatggc gagcatcgga atcggctcga caggcgcggc
      841 cgtcgtggtg gtctccggcg ggagggtcgt ggtcggctcg ggaacgacgg actcgaccga
      901 ctcgacgggc acgacgggaa cgtccggttc ggtcggattg cttccggccg cgccgtcacc
      961 gtccgatcct gagacagtcg gtgtcgtggt ggtcgtggga gtggtcgacg ccaggatgtc
     1021 gtcgagttcg tgcacggcgt cctcgttcgc agcgaggctc cgttcgacct ccgacgggcg
     1081 gtccttcgct gtcgcgacct ggtagccggc gaagacgggg aggaggagca cgacggcgag
     1141 accgacgaac acccggcggc gacgcattgc gatgcgagcc ggcaccggct tctgcagcga
     1201 gcggatccac cgcatgacgg tgcgctcccg gaacggtcgc tcgatcatgc ggaacgatgc
     1261 ctcggccagc gcgaacgtga cggcgaaccg gacggccatc acgagccacc ctggccaatc
     1321 gacgtcgccg ccctcgtcca ccgcgccggg acgcagcagc atgaacaccg gccagtgcca
     1381 cagatacaac ccgtaggagc gggtgccgag ccagaccagg ggggcgatgc cgagcacccg
     1441 ctggccgagg aacgacctca ggtgcgtcgt gcaggcgatg accatcacgg tggccagccc
     1501 gaccagcagg aacccgccgc ggtacagcgc gtcgtacccg cgtatctcgc cgtcgacgat
     1561 gacgacgtcg tggaacttcc agtgcgtcag cgccagcatc aggagtccgg cgagaccgac
     1621 gagatcgagg atccgcccct tcgtccgcaa cggactccgc atgatcgcgt acgggcgcca
     1681 caccgtggcg agcgcggccc cgaggaggag cccgccggcc cgcgtgtcgg tgccgaggta
     1741 gacccggttc gggttgagcg ggtcgtagag caccatcatc cagaccgtcg atgccagcgc
     1801 agcaacgaag aacgcgaggc cgaccttcgg caaccgctcg cggaagacgc gcagcacgac
     1861 gaccatcacg aggggccaca cgatgtagaa ctgctcctcg accgccaggg accacaggtg
     1921 gcgcaggagc tggggccggc cctggttgtc gaagtaggac aggttctcgt tgatctggaa
     1981 ccagttgctc ccgtagaaga acccccagaa gagctcgtca cgcagcttcg ccagcgactc
     2041 gctgagatag ggcagcaccg ccgacacggt ggcgacggcg acgagcagag cgatcagcgc
     2101 gggcaatagg cgccgggcgc gccgggccca gaagccgctc aggctgaggc gaccacgtcg
     2161 ttcctgttca ccgatcaaca gcaacgtgat gaggtagccg ctgatgacga agaacacctc
     2221 gacaccgagg aagccgcccg gcatccacga gacgccggcg tggtagagca tgaccgccac
     2281 caccgcgagc gcgcgcaaac catcgagccc cggaagatac gggatccgac tgatcggtgc
     2341 ctttcgcggc gcggtcggcg ggggccaggc tttggccatc ggcgccagta tcgcaaggcc
     2401 gccggacgaa tcgcaggcat cccctcgccg agccggcgct caggcgggga tcggcagacc
     2461 gaggttggcg tagatgtcgc gggtcgcgct cgagcggttc aacgtgtaga agtgcaggcc
     2521 cggcgcacct gcatcgagca gtccggcgca gagctcggtg gcgatctcga tcccgatccg
     2581 cgccgactca tcgacatcgt caccggcttt gccgacgcgc tcgac
//
LOCUS       DATDBZ010000277         3345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_500151, whole genome shotgun sequence.
ACCESSION   DATDBZ010000277 DATDBZ010000000
VERSION     DATDBZ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500151"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VIX62_06805"
     CDS             complement(<1..340)
                     /locus_tag="VIX62_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEY5859975.1"
                     /translation="MQRLDLYGTDKTVDITEPELLEAIRKVLSGAGPEVRVGVGDDAA
                     VVAPGSGELVLTTDVMVEGTHFRSGLTTARDLGAKAMTVSLSDVAAMAASPRYALCAL
                     TLSDRVDAAWV"
     gene            463..1962
                     /locus_tag="VIX62_06810"
     CDS             463..1962
                     /locus_tag="VIX62_06810"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEY5859976.1"
                     /translation="MAEVHVNPTPEQLRAFTEEMPETRISEFGNTNTQTQVLSRSAAS
                     THVVDRESSGKTMTRAQYDEIAALQDAYIAEHDMVQIDGYIGNDPEMRTAARLLMEKR
                     YANIAGMQQKLYFERDDGAEPQVQVIYTPGLKAEGWPDDRLIAVDLDHYVTRVLNSDY
                     FGESKKGGLRMWNDIVYHKGGLALHAGLKVIPTDDGDKVFMIIGLSGTGKTTTTFTTQ
                     NGSKPIQDDFVGLMPGGKAYGTENGCFAKTFALDPDFEPSIHGAVIKPSTYMENVYQA
                     EDGTVDFNNEHYTKNGRAVFSMSDLLSYEDAQNVGTVDYLLILNRNENIIPAVAKLSQ
                     EQAAAYFMLGETTGTAAGGAAEEGKFLRVPGTNPFFPLPHGLQGNRLLELLATHPIET
                     YLLNTGRVGGKDGDDRSKKLKIPDTSACVKGIAEQSIVFEEDPDFGYEVASSVPGLDD
                     PEKLQPRRLYEAQGRSEDYDAIVERLKTERVAHLQQFTQLSEEIIKAVG"
     gene            complement(2037..2843)
                     /locus_tag="VIX62_06815"
     CDS             complement(2037..2843)
                     /locus_tag="VIX62_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5859977.1"
                     /translation="MFRVELAKAFHRWRTYLLAAAIGGIPIIIVFALKVSPPDPGAAE
                     DAPPFLFQITANGLFAALTGLAVVQPFFLPLATGLFAGDAVAGEAQSGTLRYLLLRPV
                     RRTRLVLAKYCSSMTLLGGLVLVTLVSGVVAGAVVFGLSPLPTLSGSTLSVTEGLARV
                     GASGVYMLAVLSGIACIGLCISTRTDSGPGAAVATIVIAIASQIMDQIPSLDAIHPFL
                     PTHGWLGFTGLFRFPVDWAPMRAGLAVSAAYTVVFLTAAVAGFRRRDVTS"
     gene            complement(2830..>3345)
                     /locus_tag="VIX62_06820"
     CDS             complement(2830..>3345)
                     /locus_tag="VIX62_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5859978.1"
                     /translation="RLGIGLALLGAPELLVLDEPTNGLDPSGMREMRLLLRRLADDGT
                     TIFVSSHLLAEVEAMCDRVGVMAYGRLVALGPPGSLRGDADGVRLEVDDAPAAAAVVR
                     AFDGVSLIDDADPPSGQGVLRVSVADADTVAARNAALVGAGIGVHALVPERSSLEDVF
                     LSLVEGADVPR"
BASE COUNT          648 a         1215 c         1063 g          419 t
ORIGIN      
        1 tcacccaggc ggcgtccaca cgatcggaca gcgtaagcgc gcacagggcg tatcgagggc
       61 tcgcggccat cgccgccacg tcgctcaagg agacggtcat cgctttagcg ccgagatcac
      121 gcgccgtcgt cagcccgctt cggaagtggg tgccctcgac catcacgtcg gtggtgagga
      181 cgagctcccc cgagcccggt gccaccaccg ccgcgtcgtc gcccacaccg acccgcacct
      241 cggggccggc gccggacagg accttgcgga tcgcctccag cagctctggt tccgtgatgt
      301 ccaccgtctt gtccgttccg tacaagtcga gccgctgcat cgcagccagt tgcgccgaag
      361 gaggcctgaa cgccgttcgg tagactctca gccgtactcc ggcatgtcca aacggccggg
      421 tcacgcgcaa ccccaagcat cccgcaacga ggaggcccca tcgtggcaga ggttcacgtc
      481 aacccgaccc cggaacagct tcgggcgttc acggaggaga tgcccgagac gcgcatctcc
      541 gagttcggga acaccaacac ccagacccag gtgctctccc gcagcgccgc cagcacgcac
      601 gtggtcgacc gcgagtccag cggcaagacc atgacgcggg cccagtacga cgagatcgcc
      661 gcgctgcagg acgcctacat cgccgaacac gacatggtcc agatcgacgg ctacatcggc
      721 aacgaccccg agatgcgcac cgccgcccgc ctgctgatgg agaagcgcta cgccaacatc
      781 gccgggatgc agcagaagct ctacttcgag cgcgacgacg gagccgagcc gcaggtccag
      841 gtgatctaca cgccgggcct caaggccgag ggctggcccg acgaccgcct catcgccgtg
      901 gatctcgacc actacgtgac ccgcgtcctc aactccgact acttcggtga gtcgaagaag
      961 ggcggcctac ggatgtggaa cgacatcgtc taccacaagg gcggcctggc gttgcacgcc
     1021 ggcctcaagg tgatccccac cgacgacggc gacaaggtgt tcatgatcat cggcctgtcg
     1081 ggcacgggca agaccaccac cacgttcacg acgcagaacg gttccaagcc gatccaggac
     1141 gacttcgtgg ggctgatgcc gggcggcaag gcctacggca ccgagaacgg gtgcttcgcc
     1201 aagacgttcg cgctcgaccc cgacttcgag ccctcgatcc acggcgccgt gatcaagccg
     1261 agcacctaca tggagaacgt ctaccaggcc gaggacggca cggtcgactt caacaacgag
     1321 cactacacga agaacggtcg cgcggtcttc tcgatgagcg acctgctctc gtacgaggac
     1381 gcgcagaacg tcggcacggt cgactacctg ctgatcctca accggaacga gaacatcatc
     1441 ccggcggtgg cgaagctctc gcaggaacag gccgcggcgt acttcatgct cggggagacc
     1501 accggcaccg ccgccggcgg cgcggccgaa gaaggcaagt tcctgcgcgt tcccggcacg
     1561 aacccgttct tcccgctccc tcacggactg cagggcaacc ggttgctcga actgctggcc
     1621 acccacccga tcgagaccta cctgctcaac acgggccggg tcggcggcaa ggacggcgac
     1681 gaccgttcca agaagctgaa gatcccggac acctccgcgt gcgtgaaggg catcgccgag
     1741 cagtcgatcg tcttcgaaga ggaccccgat ttcggctacg aggtggcctc ctcggtcccc
     1801 ggactcgacg accccgagaa gctccagccg cgccggctct acgaggcgca gggtcgcagc
     1861 gaggactacg acgccatcgt cgaacgcctc aagaccgagc gcgtcgccca cctgcagcag
     1921 ttcacgcagc tgtcggagga gatcatcaag gccgtcggct gacgcaccgc cggaacgacg
     1981 cctgaccgca cgagccgggg cgcgacgccc cggctcgttc gtgttcgatc ccggcctcag
     2041 ctcgtgacgt ctcgccggcg aaaccccgcg accgccgccg tgaggaacac cacggtgtag
     2101 gcagcgctca ccgcgagccc ggcgcgcatc ggcgcccaat ccaccgggaa gcggaacaac
     2161 ccggtgaacc cgagccagcc gtgcgtcggc aggaacggat ggatcgcgtc gagactgggg
     2221 atctgatcca tgatctgact cgcgatcgcg atcacgatgg tcgccacggc cgcgccggga
     2281 ccggaatcgg tccgcgtcga gatgcacagc ccgatgcacg cgatccccga cagcaccgcg
     2341 agcatgtaca cgcccgaggc cccgacgcgt gcgagccctt cggtcaccga cagggtggat
     2401 cccgacaggg tgggcaaagg actcaacccg aacaccacgg caccggccac cacgccggag
     2461 acgagcgtga cgaggaccag tcctcccaac agcgtcatcg acgagcagta cttcgccagg
     2521 accagccggg tgcgacgcac cggtcgcagc agcaggtagc gaagggtccc cgactgtgcc
     2581 tcgcccgcca cggcgtcccc ggcgaacaac cccgtcgcca gcggcaggaa gaacggctgg
     2641 accaccgcca ggcccgtcag cgccgcgaac agtccgttgg ccgtgatctg gaacaggaac
     2701 ggcggggcat cctcggcggc gcccgggtcg ggcggggaca ccttcagcgc gaacacgatg
     2761 atgatcggga tgccgccgat cgcggccgcc aacaggtacg tgcgccaccg gtggaaggcc
     2821 ttcgccagct caacgcggaa catcggcgcc ctccacgagc gagaggaaga cgtcctcgag
     2881 cgaggagcgc tcgggcacga gcgcatggac gccgatccct gccccgacca gtgccgcgtt
     2941 gcgcgcggcc acggtgtcgg cgtcggccac cgacacccgc agcacgccct gcccgctcgg
     3001 tgggtccgcg tcgtcgatca aggacacgcc gtcgaacgca cggacgaccg cagcggcggc
     3061 gggggcatcg tccacctcga gccgcacgcc gtcggcgtca cctcgaaggc tcccgggtgg
     3121 cccgagcgcc accaggcgcc cgtaggccat cacgcccacc cgatcgcaca tcgcctcgac
     3181 ctcggcgagc aggtggctcg agacgaagat ggtggtgccg tcgtcggcca gccgacgcag
     3241 gagcaagcgc atctcccgca taccgctggg atcgagacca ttggtgggct cgtcgaggac
     3301 caacaactcc ggagcgccca acagcgccag cccgataccg agacg
//
LOCUS       DATDBZ010000278         2042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_67233,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000278 DATDBZ010000000
VERSION     DATDBZ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67233"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VIX62_06825"
     CDS             complement(<1..375)
                     /locus_tag="VIX62_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY5859979.1"
                     /translation="MDTLISILHVTTAVFIVGPMAILPMTAMRAVRAGNGGQVAVLAK
                     STMVFSWLSLLTFVFGFGAMAMAPEEFELTFTTPWILWSIILYVIAFVLSVFVVVPAM
                     RRAAEALSGDAGSGGGAAQAGYP"
     gene            658..2022
                     /locus_tag="VIX62_06830"
     CDS             658..2022
                     /locus_tag="VIX62_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016823804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5859980.1"
                     /translation="MSAPSAAPAAPTTTPANPRSRVVLASLIGTTIEFYDFYVYATAA
                     VLVFPHLFFPTGDATTALLSSFAVFGAAMIARPLGAVIFGHLGDKHGRKATLVGALLT
                     MGIATFLIGLLPTYAMVGWLAPLLLVILRIAQGFALGGEWSGAALVATENAPEGKRAL
                     FGTFPQLGAPIGFIIANGLFLVIAAMLPSDDPSMPSQAFLDWGWRIPFLFSVVMVIVG
                     LWVRLRLVESTAFSNASKQGKLTRLPLASVFKNHWKHLILGTLFMLATYVLFYLMTTF
                     SLSYGRAPQQAAEGALPGLGYSYTTFVLMLIGGVVFFGIFTLVSGPWADRWGRRKTLI
                     WVTLGIITFGLLWVPLLGAGFWGVMLWLVVGFSLMGLTFGPMGALLPELFPTNVRYTG
                     SGLTYNVSSILGAAVAPFIAVALWQAGDGSPFWVGIYLSAMATLTLIALLISKETKDI
                     DINA"
BASE COUNT          291 a          759 c          623 g          369 t
ORIGIN      
        1 cgggtagccc gcctgcgctg cgccgccgcc cgatccggcg tcgcccgaca gtgcttcggc
       61 ggccctgcgc atcgccggga cgaccacgaa gacgctgagc acgaacgcga tcacgtagag
      121 gatgatcgac cacaggatcc agggggtcgt gaacgtgagc tcgaactcct cgggcgccat
      181 cgccatcgcg ccgaatccga acacgaacgt gagcagcgag agccagctga acaccatcgt
      241 cgacttcgcg agcacggcga cctgaccccc gttgccggcg cggaccgcgc gcatcgcggt
      301 catgggcagg atcgccatcg gcccgacgat gaagacggcc gtggtgacat gcagaatcga
      361 gatcagggta tccatgccgc ccagcctagc cacgcggcat ccggggtcgc gtcgaacctt
      421 cgatcgatct ggcgccccgg ttcggagccg gtaaggactc ccccgcaccg ttcaccgcac
      481 cgagcgcacc gctcaccgca tgatgccacc gccatgcgcc tcggtgcatg cggtgttgcg
      541 tagggtggtg aggttgccac aagcgacgat tcctgcactg cctccggtcc gaacgcctga
      601 ccgacgtgcc tcggggcgca gcgttcttcc gcacccccat tggagtcttc gccagcaatg
      661 tctgcaccat ccgcggcccc ggccgcaccc accaccactc ccgccaatcc gcgcagccgt
      721 gtcgttctcg cgagcctcat cggcacgacg atcgagttct acgacttcta cgtctacgcg
      781 accgccgccg tgctggtctt cccccacctg ttcttcccga cgggcgacgc gaccaccgcc
      841 ttgctctcgt cgttcgccgt cttcggcgcc gccatgatcg cgcgaccgct cggcgccgtc
      901 atcttcggcc acctgggcga caagcacggc cgcaaggcga cgctcgtcgg cgcgctgctc
      961 acgatgggca tcgcgacctt cctgatcgga ctcctgccga cctatgcgat ggtcggatgg
     1021 ctcgcacccc tgctgctcgt catcctgcgc atcgcccagg gcttcgcgct cggcggcgag
     1081 tggtcgggcg ccgcgctcgt ggccaccgag aacgctcccg aaggcaagcg cgccctgttc
     1141 ggcaccttcc cgcagctcgg cgcacccatc ggcttcatca tcgcgaacgg cctgttcctc
     1201 gtcatcgcgg cgatgctccc ctccgacgac ccctcgatgc cgtcgcaggc gttcctcgac
     1261 tggggctggc gcatcccgtt cctgttctcg gtcgtcatgg tcatcgtcgg cctctgggtg
     1321 cgcctgcgcc tggtcgagtc caccgccttc tcgaacgcgt cgaagcaggg caagctcacc
     1381 cgcctgcccc tcgccagcgt cttcaagaac cactggaagc acctcatcct cggcacgctc
     1441 ttcatgctcg ccacgtacgt gctcttctac ctgatgacca ccttctcgct ctcgtacggc
     1501 cgtgccccgc agcaggcagc cgagggcgcg ctccccggtc tcgggtattc gtacaccacg
     1561 ttcgtgctca tgctcatcgg cggtgtcgtg ttcttcggca tcttcaccct ggtgtcgggc
     1621 ccgtgggccg accgctgggg gcgccgcaag acgctcatct gggtcacgct cggcatcatc
     1681 acgttcggcc tgctgtgggt gccactgctc ggcgccggat tctggggcgt gatgctctgg
     1741 ctcgtcgtgg gcttctcgct catgggactg acgttcggcc cgatgggcgc gctgctgccc
     1801 gagctgttcc cgacgaacgt gcgctacacc ggctcgggcc tgacgtacaa cgtgtcgtcg
     1861 atcctcggcg cggcggtcgc cccgttcatc gcggtggccc tgtggcaggc cggcgacggc
     1921 agcccgttct gggtcggcat ctacctctcg gcgatggcga cgctcacgct catcgcgctg
     1981 ctcatctcga aagagacgaa ggacatcgat atcaacgcct gagtgcggtt cgagacgagg
     2041 gg
//
LOCUS       DATDBZ010000279         3897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2143921, whole genome shotgun sequence.
ACCESSION   DATDBZ010000279 DATDBZ010000000
VERSION     DATDBZ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143921"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VIX62_06835"
     CDS             complement(<1..1020)
                     /locus_tag="VIX62_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5859981.1"
                     /translation="MSTKSTSVPAEIVTAMGAPPTDEQWAAISMPLQPYVLVAGAGSG
                     KTSVMAARVVYLALAALGRIEAPTGSLANPGVLPGNVLCLTFTVKATENLRSRVRRAL
                     ASIDLAEGEEPQVLNYHAMAAQVLERYGILAGIETGGRVLSQAQRVELAGRVLDEMDF
                     EHTKTEWQPSVVDKILNLDAQIANHLLDPDEVVAFVEERLPVYKKIARSDRVYFSALE
                     RIELAKASVIFRRLKRDLGVIDFSDQIELAMKIAGSHPEVGREYRDRFGAVLLDEYQD
                     TNVAQARLMEALFGEGHPVTAVGDPDQNIYAWRGASLYNLLEFPSAFRSADGTEAPRL
                     PLFTNF"
     gene            complement(1017..>3897)
                     /locus_tag="VIX62_06840"
     CDS             complement(1017..>3897)
                     /locus_tag="VIX62_06840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY5859982.1"
                     /translation="FSVLRHDAGSLLVIGAPGTGKSAILRERFAALIEGGADPERVAL
                     VLGSSASRAAARASLHARLPASLPGLNVVTLHGLANRVLKDRLEAAGDEPPQILSAAE
                     QFARVQELLTDQDPEAWPAYGTLLGLRGFADEVRQFLLRAQEGLRTPDDIVAAADRRG
                     LTGWHELARFLGEYQTVMDDLGVVDFAGLVQRAASAAGEGAPLFDHVLVDDYQDTTLA
                     GEAVIRGLRAPDLVVAADPEAHVFSFQGTTRVPLDRFASEAFDGAATITLETNHRTDG
                     EPVQHLAWVAPHPSEEHAAIARELRRLHVEEGVGWADMAVVVRRQGSVLGGLLRALGD
                     ARIPRAIPERGLSLTAEPATRPYVLALRWLVADPTQREELVEQLVTSDVVGLSPAVAR
                     GLLRAARADTGSIANALDVDEGLTADELERVQVARETLAKAALFAGMSVQDAFRTLWD
                     SLACSRHLVERADEDAEARRDLDTVVMFANVVSETDAAGGDKSVQAFLESLDAGEHGP
                     GYSAWERSRADAVQVLTAHGTVGREFDTVVVTGVTEGNFPSVSRPEPMFDLAVLDHPI
                     SNAERNRTRLEDERRLFDMVVARASRRVVLTCADPHPASDADAVSSRSRFVPADTWGP
                     APAGPFEEPVSTREAAASWRHELADPGLPGWRRLAALDGLVALDVEPRRWWFQRDWTD
                     TGRPLHETIRVSYSRLSNLEACELMHVLGDELGLGRPGGYHAWVGKTVHRIIEEVERG
                     EIPKEPRAMIEVLHQRWRPQEFPSRAVSEAFRKLARESMLKNWFDTYGDKPSLAIEQY
                     FEFEFEGVTVLGYIDRIGPAVQDGTVITDYKTGKSDNAGPPQESLQLGIYYLAVQDSD
                     VLADYQPVRTVELAFLRGNWRRSDAADFRKLPITERDEETYEAGMRERLASLIARKKE
                     LNEIEVFRPNPYANCRFCDFQTLCPLYPEGQPVFPVESVRLGASA"
BASE COUNT          581 a         1318 c         1400 g          598 t
ORIGIN      
        1 gaagttggtg aacagcggca gtcggggtgc ctcggtcccg tcggcgcttc ggaacgccga
       61 ggggaattcc agcaggttgt agagcgacgc tccgcgccac gcatagatgt tctggtcggg
      121 gtcgcccacg gcggtgacag gatggccctc gccgaacaag gcctccatga ggcgggcctg
      181 cgcgacgttc gtgtcctggt actcgtccag cagcacggcg ccgaaccgat cgcggtactc
      241 ccggcccacc tcggggtgcg aaccggcgat cttcatcgcc agctctatct ggtcgctgaa
      301 gtcgatgacg cccaggtcgc gcttcagccg ccggaagatg accgaggcct tcgcgagttc
      361 gatgcgctcg agcgcgctga agtacacgcg gtcggagcgc gcgatcttct tgtagaccgg
      421 aaggcgttcc tcgacgaagg ccacgacctc gtcggggtcc agcaggtggt tcgcgatctg
      481 ggcgtccagg ttcaggatct tgtcgacgac gctgggctgc cactcggtct tcgtgtgttc
      541 gaagtccatc tcgtccaaca cgcggccggc aagttccacc cgttgagcct ggctgagcac
      601 gcgtccgccg gtctcgatac cggcgaggat gccgtagcgc tccagcacct gggcggccat
      661 ggcgtggtag ttgaggacct ggggttcttc accttcggcg aggtcgatgc tggccaacgc
      721 gcgacggacc cgcgaccgca ggttctcggt ggccttcacc gtgaaggtga ggcacagcac
      781 gttgccgggc aggacccccg ggttggcaag agacccggta ggcgcctcga tgcgaccgag
      841 cgcggcgagc gcgaggtaca cgacgcgcgc cgccatgacg ctggtcttgc ccgaacccgc
      901 gccggcgacc aacacgtacg gctgcagcgg catggagatg gcggcccact gctcgtcggt
      961 ggggggcgcg cccatggcgg tgacgatctc ggcggggaca ctggtcgact tcgtgctcat
     1021 gcactggccc ccagccgcac ggactccacg gggaacacgg gctggccctc ggggtagagc
     1081 ggacacagcg tctggaagtc gcagaaccgg cagttggcgt acgggttggg ccggaacacc
     1141 tcgatctcgt tgagctcctt cttcctcgcg atcagggacg cgaggcgctc gcgcatgccg
     1201 gcttcgtagg tctcctcgtc gcgttcggtg atggggagct tgcggaagtc ggcggcgtcg
     1261 gaacgacgcc agttcccgcg caggaacgcc agctcgacgg tgcggaccgg ttggtagtcg
     1321 gcgagcacgt cggagtcctg aacggcgagg tagtagatgc cgagttgcag gctctcctgg
     1381 ggcggcccgg cgttgtcgct cttgccggtc ttgtagtcgg tgatgaccgt gccgtcctgc
     1441 accgccgggc cgatccggtc gatgtagccc agaacggtga ccccttcgaa ctcgaactcg
     1501 aagtactgct cgatcgcgag cgagggcttg tcgccgtagg tgtcgaacca gttcttcagc
     1561 atggactcgc gtgccagctt gcggaacgcc tccgacacgg cacgcgaggg gaactcctgc
     1621 ggccgccagc gctggtgcag cacctcgatc atggcgcgag gctccttggg gatctcgccg
     1681 cgctccacct cttcgatgat gcggtgcacg gtcttgccca cccaggcgtg gtagccaccg
     1741 ggcctgccga ggccgagctc gtcgcccaac acgtgcatga gctcgcacgc ctccaggttg
     1801 gacaaccgcg agtacgagac gcggatcgtc tcgtgtagcg gacgtccggt gtcggtccag
     1861 tcgcgctgga accaccaccg tcggggctcg acgtcgagcg ccacgagtcc gtcgagcgcg
     1921 gcgaggcgtc gccagccggg gagcccgggg tcggcgagct cgtgccgcca actggccgcg
     1981 gcttcccgtg tggacaccgg ttcttcgaac ggtccggcgg gagccggtcc ccatgtatcg
     2041 gcgggcacga acctggacct gctggacacg gcgtcggcat cggaggcggg gtgcggatcg
     2101 gcgcaggtca ggaccacgcg acgggacgcc cgggccacca ccatgtcgaa caaccggcgt
     2161 tcgtcctcga gccgggtgcg gttgcgctca gcgttcgaga tggggtgatc gagcacggcg
     2221 agatcgaaca tcggctctgg gcgcgagacg ctggggaagt tcccctcggt gacaccggtc
     2281 accaccaccg tgtcgaactc gcgtcccacc gtgccgtggg cggtgagcac ctgcaccgcg
     2341 tcggcgcgcg agcgttccca cgccgaatag ccggggccgt gctcgcccgc gtcgagggat
     2401 tcgaggaacg cctgcacgct cttgtcgccg cccgcggcgt cggtctcgga caccacgttc
     2461 gcgaacatga cgacggtgtc gaggtcacgg cgcgcctcgg cgtcctcgtc ggcgcgctcc
     2521 accaggtgcc gggagcacgc gaggctgtcc cacagggtgc ggaacgcgtc ctgcacgctc
     2581 atcccggcga agagtgccgc cttggccagg gtctcgcggg ccacctggac gcgctcgagc
     2641 tcgtcggcgg tgagtccttc gtccacgtcg agcgcattgg cgatcgatcc ggtgtcggcg
     2701 cgggcggctc gcagcagtcc gcgagcgacg gcgggggaga gtcccaccac atcggacgtg
     2761 acgagctgtt ccaccagttc ctcacgctgg gtgggatcgg ccaccagcca acgcaacgcg
     2821 aggacgtagg gacgcgtggc gggctcggcg gtgagcgaga ggcctcgttc ggggatcgct
     2881 cgcgggatgc gcgcgtcccc gagcgcccgg agcagcccac cgagcaccga accctggcgc
     2941 cggaccacca cggccatgtc cgcccagccc acgccttcct cgacgtgcag gcggcgcagc
     3001 tcgcgggcga tcgcggcgtg ttcctcggac ggatgcggcg cgacccacgc cagatgttgc
     3061 accggctcgc cgtcggtccg atggttcgtc tcgagcgtga tcgtggccgc gccgtcgaac
     3121 gcctcggagg cgaaccggtc gagcgggacc cgggtggtcc cctggaacga gaagacgtgg
     3181 gcctcggggt cggccgccac gacgagatcg ggcgcgcgca acccgcggat caccgcttcg
     3241 ccggccaggg tggtgtcctg atagtcgtcg acgagcacat ggtcgaacaa cggcgcgccc
     3301 tcgccggccg cggaggccgc gcgttggacc agtccggcga agtcgaccac ccccaggtcg
     3361 tccatcacgg tctggtattc cccgaggaac ctggcgagct cgtgccagcc ggtgaggccg
     3421 cgccggtcgg cggcggccac gatgtcgtcg ggcgtccgca gtccttcctg cgcacgaagg
     3481 aggaactggc ggacctcgtc ggcgaacccg cgcaagccga gcagcgttcc gtaagcgggc
     3541 caggcctccg gatcttggtc ggtgagcagt tcctgcacgc gggcgaactg ctcggccgcc
     3601 gacaggatct gcggcggctc gtcgccggcg gcctccaacc ggtccttcag cacgcggttg
     3661 gcgagaccgt gcagggtgac cacgttgagt cccggcaggg aggcgggcag cctggcgtgg
     3721 agggaggcgc gagccgccgc tcgtgacgcc gacgacccga ggaccaacgc cacccgctcc
     3781 gggtcggcac cgccttcgat cagcgccgcg aaccgttccc ggaggatggc cgacttgccc
     3841 gtccccgggg cgccgatgac caggagggag cccgcgtcgt gccggagcac gctgaac
//
LOCUS       DATDBZ010000280         4452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_912141, whole genome shotgun sequence.
ACCESSION   DATDBZ010000280 DATDBZ010000000
VERSION     DATDBZ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912141"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2381
                     /locus_tag="VIX62_06845"
     CDS             <1..2381
                     /locus_tag="VIX62_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5859983.1"
                     /translation="PSWRLKDETARTMPHRFREIALESLSGDSGSALLTALVGHDTLP
                     AATAERVLASADGNPFFLEEIVRSLQDAGALVPTEEGLRFDHEVDVEIPPTVEKVILA
                     RIDRLEPSPRQALLAASVLGRRFSLSLLEGVADDSGIRSALSELQRLDFVREGRRWPE
                     PEFRFKHALIQEAAYRTLVTEDRTRLHRKAAETLEAQRGEGSREIAGLLAHHWLGAAD
                     EDKAVRYLTLAGDRARQEYALDEAVAYYRELLPLLERRGEGQEIALGLFKLALALHMA
                     LRFSEADATYQRAFEHWTPPAPVETNATLRIASSFLPNDPDPRSAIAWPNIQLCMQLF
                     DRLVEQWPERTIVPSLAERWDISPDGLRYVFHLREGLRWSDGTPLTAGDVEFGIQRVL
                     DPDAPGSSVAVYFVLEHGQERYLGSPEEDRIGVRALDDRTVEFRLVAPAPYFLSVMNR
                     PDGGPQPRHAIVGHEGDWAQPGSQVVSGAFRVDEPGSDRLVLRRRDDYTGPRGGNVER
                     VEIVAAPIATSVEAFARDEVDLVSVRYTPRLADVAPADVPDARLGPAAWSGYLAFDHQ
                     QGPTSGVDLRLALAHAVDRDAISAILPINMVAATGGIVPPALQGHTPDISLRFDPDLA
                     REALGRSGFVGALSVACLDEDEMLVAPVLESWRTILGLDVQVRPWRRSDIATMPPPRQ
                     LAPIYLTGWLPGYADPEYFLRLLFQSDSRTNEGGFASEAFDQLIERARQERSDRSRLE
                     LFHQADRMAVVERVAVIPVVYGRSMSQVKPRVHGWWEFGKSSANFADLLLDP"
     gene            complement(2413..3090)
                     /locus_tag="VIX62_06850"
     CDS             complement(2413..3090)
                     /locus_tag="VIX62_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY5859984.1"
                     /translation="MTAPAVVLFDIDGTLISTGGAGARSWGRAFRKLHDREADITAFS
                     ESGMTDPVVGRAVFEGVLGRQPNQQELSRLIMAYLMELPIEVANSPTYHRFDGVIRTL
                     ERLSEAGTLLGLVTGNIEGAARIKIDRANLNPYFAFGGYGTDSADRAELTLAAIARAE
                     TMQGTAIERSTIVVVGDTPRDVAAAKAVGAVSVAVATGDYSVDQLRETGADHVLRNFA
                     EDEFPGL"
     gene            complement(3092..3730)
                     /locus_tag="VIX62_06855"
     CDS             complement(3092..3730)
                     /locus_tag="VIX62_06855"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY5859985.1"
                     /translation="MTFIDISLPIGPDLLTWPGNPEVTVTPFQRIADGDASNVSELRI
                     GTHSGTHVDPPVHFVEGGAGIDRVALDVLVGECVVVDARGRQGELTAEDMEALAIPAD
                     AERVLLRTDNSDLWRRLPVEFPQGYVCVGPDAARWVVAHGIRLIGIDFLGIERQGSPG
                     HPTHHELLSHGVVIVEGLDLGMADPGPYRLTVLPLRIVDGDGGPARAVLETR"
     gene            complement(3730..>4452)
                     /locus_tag="VIX62_06860"
     CDS             complement(3730..>4452)
                     /locus_tag="VIX62_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY5859986.1"
                     /translation="HMLQVIAFLAMEPPRSLDAEAFRDEKVKLLRAIRPIDPADVVRG
                     QYDGYRQEEGVDPKSQVETFIAARMWIDNWRWDGVPFYLRHGKKLPIRNTEITVVFRQ
                     APDYLFRDLELEHIPSNHLTIRVQPDEGISLAFQAKTPGPGYHLQTVRMDFDYDESFA
                     DASAEAYERLLHDAMDGDHTLFNRADSVDRAWEIVTPMLEEPPPLFHYAQGSWGPSRA
                     DRLIEPHEWHLRTRADGTMGHH"
BASE COUNT          647 a         1533 c         1550 g          722 t
ORIGIN      
        1 acccctcgtg gcgattgaag gacgagacgg cgcggacgat gccccaccgg ttccgagaga
       61 tcgcgttgga gtccctgtcc ggcgactcgg gctccgcact cctgacggcg ctggtcggcc
      121 acgacacgct gccggcggcg acggccgaac gcgtgctcgc gtccgccgac ggcaacccct
      181 tcttcctgga agagatcgtg cgctcgttac aggacgccgg ggccctcgtg cccaccgagg
      241 aggggctgcg cttcgatcac gaggtcgatg tggagatccc acccaccgtg gagaaggtga
      301 tcctggcccg gatcgaccgg ctcgaaccgt cgccacgcca ggcgttgctg gccgcctcgg
      361 tcctcggccg tcggttcagc ctctcgctcc tggaaggggt cgcggacgac tcggggatcc
      421 gctcggcgct gtcggagctg cagcggttgg acttcgtgcg cgagggccgc cgatggcccg
      481 aacccgagtt ccgtttcaag cacgccttga tccaggaggc cgcctatcgc acgctcgtga
      541 ccgaggatcg aacccggttg caccgcaagg cggccgagac cctggaggcc cagcgaggcg
      601 agggctcgcg agagatcgcg gggttgctgg cccaccactg gctcggcgcc gccgacgagg
      661 acaaggccgt ccgctacctc accttggcgg gcgaccgtgc ccgtcaggag tacgcgctcg
      721 acgaggcggt cgcctactac cgggagctgc ttcccttgct ggagcgccgc ggtgaggggc
      781 aggagatcgc gctcggtctg ttcaagctgg cgctcgcctt gcacatggcg ctgcggttct
      841 cggaggccga cgcgacctac cagcgggcct tcgagcattg gaccccgccg gcgccggtcg
      901 agacgaacgc caccctgcgc atcgccagca gtttcctgcc caacgacccg gatccccgtt
      961 cggcgatcgc ttggccgaac atccaactgt gcatgcagct gttcgaccgg ctcgtcgagc
     1021 aatggccgga gcgcacgatc gtgccgtcgc tcgcggaacg ctgggacatc tcccccgacg
     1081 gcctgcgcta cgtcttccat cttcgggaag gcctgcgctg gtccgacggc acaccgctca
     1141 cggcgggtga cgtcgagttc ggcatccaac gggtgctcga tccggacgcc ccgggatcgt
     1201 cggtcgccgt gtacttcgtg ttggaacacg ggcaggagcg atacctcggc tccccggagg
     1261 aggaccgcat cggtgtgcgc gcgttggacg accgcaccgt ggagttccgg ctggtggcgc
     1321 ccgcaccgta cttcctgagc gtgatgaacc gtccggacgg gggcccgcaa ccccgccatg
     1381 cgatcgttgg tcacgaaggg gactgggcac agcccggatc gcaggtggtg tcgggggcgt
     1441 tccgcgtcga cgaacccgga tccgaccgcc tcgtgctgcg gcgacgtgac gactacaccg
     1501 gtccgcgagg cggcaacgtc gagcgcgtgg agatcgtcgc cgcacccatc gccacctccg
     1561 tcgaggcgtt cgcccgtgat gaggtggacc tggtgtcggt gcggtacacg ccccgactcg
     1621 ccgatgtcgc cccagcggat gtgcccgacg cccggctcgg accggcggcg tggtcgggtt
     1681 acctcgcgtt cgatcaccag caaggcccga cctccggcgt ggacctgcgt ctggccctgg
     1741 cccacgcggt ggatcgcgac gcgatctcgg cgatcctgcc gatcaacatg gtggcggcca
     1801 ccgggggcat cgtgcccccc gcgctgcagg gccacacgcc cgacatctcg ctgcggttcg
     1861 atcccgatct ggctcgggag gcccttgggc gttcggggtt cgtcggggcg ctgtcggtcg
     1921 cctgcctgga cgaggacgag atgctggtgg cacccgtcct cgagtcgtgg cgaacgatcc
     1981 tgggcctgga cgtccaggtg cgaccctggc ggcgcagcga catcgccacg atgccgccgc
     2041 cccgacagct ggcgccgatc tacctcaccg ggtggctgcc gggctatgcc gatcccgagt
     2101 acttcctgcg gctgctgttc cagtccgaca gccgtaccaa cgagggtggg ttcgcgtcgg
     2161 aggcgttcga ccagctgatc gaacgagccc gccaggagcg gagcgaccgc agccggttgg
     2221 aactcttcca ccaggccgac cggatggcgg tggtggagcg cgtggcggtc atccccgtcg
     2281 tctacggacg cagcatgtcg caggtgaagc cgcgcgtgca cgggtggtgg gagttcggga
     2341 agtcatcggc caacttcgcc gacctcctgc tcgacccctg agctacccct gatctacccc
     2401 tgaccttggg cgctacagcc cggggaactc gtcctcggcg aagttccgca ggacgtgatc
     2461 ggcgccggtc tcgcgcagct ggtcgacgga gtagtcgccg gtcgccaccg ccacgctgac
     2521 cgcccccacc gccttcgcgg ccgcgacgtc acgcggcgtg tcaccgacca cgacgatcgt
     2581 cgaccgttcg atcgctgttc cctgcatcgt ctcggcgcgg gctatcgccg ccagcgtcag
     2641 ttcggcgcga tccgcggagt cggtgccgta tcccccgaac gcgaagtagg gattgaggtt
     2701 ggcccggtcg atcttgatcc gagcagctcc ttcgatgttg ccggtcacca ggcccagcag
     2761 cgtgccggct tcggagaggc gctcgagcgt ccggatcacg ccgtcgaacc ggtgataggt
     2821 gggcgagttg gccacctcga tcggcagttc catcagatag gccatgatca agcgagagag
     2881 ctcctgctgg ttcggttgcc ggcccagcac cccttcgaac accgcgcgtc ccacgacggg
     2941 gtcggtcatg ccgctctcgg agaacgcggt gatgtcggcc tcgcggtcgt ggagcttgcg
     3001 gaacgcgcgg ccccagctcc gagcgccggc gcctccggtg ctgatcaacg tgccgtcgat
     3061 gtcgaacaac acgacggcgg gagcggtcac gtcagcgggt ctccagcacg gcacgcgccg
     3121 ggccgccgtc gccgtccacg atccgcagcg gcaggacggt gaggcggtag gggccggggt
     3181 cggccatccc gaggtcgagc ccctcgacga tcacgacgcc atgggacagc agttcgtggt
     3241 gcgtgggatg cccgggcgag ccctgccgtt cgatgccgag gaagtcgatg ccgatcagcc
     3301 ggatcccgtg agccaccacc catcgcgccg cgtccggacc cacgcagacg tatccctgcg
     3361 ggaactcgac cgggagccga cgccacaggt cggagttgtc ggtgcggagc agcacgcgct
     3421 cggcgtcggc ggggatcgcg agcgcttcca tgtcctcggc ggtcagttcc ccctggcgac
     3481 ctcgcgcgtc caccacgacg cactcgccca cgagcacgtc gagcgccacg cggtcgatcc
     3541 cggcgccgcc ctcgacgaag tgcaccggcg ggtccacatg ggtgccggag tgcgtgccga
     3601 tccgcagttc gctcacgttc gaggcgtcac cgtcggcgat ccgctggaac ggcgtgacgg
     3661 tgacttcggg gttgcccggc cacgtgagca ggtcgggacc gatcgggagc gagatgtcga
     3721 tgaacgtcat cagtggtgtc ccatcgtgcc gtcggcgcgg gtgcggagat gccattcgtg
     3781 cggttcgatc agccggtcgg cgcgcgaggg gccccacgag ccctgtgcgt agtggaacag
     3841 cggcggcggc tcctccagca tcggcgtgac gatctcccac gctcggtcga ccgagtcggc
     3901 ccggttgaac agggtgtggt cgccgtccat cgcatcgtgc aacaggcgtt cgtatgcctc
     3961 ggccgacgcg tccgcgaacg actcgtcgta gtcgaagtcc atgcgcaccg tctgcaggtg
     4021 gtagcccggg ccgggtgtct tggcttggaa cgcgagcgag atcccctcgt cgggctgcac
     4081 gcggatcgtc agatggttcg acgggatgtg ctccagctcc aggtcgcgga acaggtagtc
     4141 gggcgcctgg cggaacacca cggtgatctc cgtgttgcgg atcgggagct tcttgccgtg
     4201 ccggaggtag aacggcacgc cgtcccaccg ccagttgtcg atccacatcc gggcggcgat
     4261 gaacgtctcg acctggctct tgggatcgac cccctcctcc tggcggtagc cgtcgtactg
     4321 accccgcacg acgtcggcgg gatcgatggg tcggatcgct cgcagcagct tcaccttctc
     4381 gtcgcggaag gcttcggcat ccagggatcg gggcggttcc atggcgagga acgcgatcac
     4441 ctgcagcatg tg
//
LOCUS       DATDBZ010000281         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_912772, whole genome shotgun sequence.
ACCESSION   DATDBZ010000281 DATDBZ010000000
VERSION     DATDBZ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912772"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VIX62_06865"
     CDS             <1..189
                     /locus_tag="VIX62_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859987.1"
                     /translation="QLGHGEGYRYPHDFPGHAVDQEYRPTTFQGKRYYEPSGQGRDVP
                     RIPGTGQKAEEETEDRDG"
     gene            268..642
                     /locus_tag="VIX62_06870"
     CDS             268..642
                     /locus_tag="VIX62_06870"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEY5859988.1"
                     /translation="MTLLAMSGDTALTIVAVFWAVLVLGLLVVLLNTFRVLESTKMSI
                     DTMREEVVPLLREVKGSVERANRELDRVDTMLISATEVVGRVEKLSGLAEQAAASPLV
                     KIISVGAGLKKGFSRARGGRKQ"
     gene            639..908
                     /locus_tag="VIX62_06875"
     CDS             639..908
                     /locus_tag="VIX62_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5859989.1"
                     /translation="MKRGFWVALGLGAGATGAIMASRWTKKQAQRVAPQSLAREAKGG
                     LLDFSKLVSESIQEGKQAMAEREAQLRADNDLPVATGEPDEPPAA"
     gene            930..>2272
                     /gene="alaS"
                     /locus_tag="VIX62_06880"
     CDS             930..>2272
                     /gene="alaS"
                     /locus_tag="VIX62_06880"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY5859990.1"
                     /translation="MEGHRIRESFLSFFEERDHKRVQSSSLIPPPESGLLLTNAGMNQ
                     FIPYFLGQASAPYPRAVTSQKVMRTNDIENVGHDARHETFFEMLGNFSFADYFKAEAI
                     TWAHELITEGYGIDHDLLWVTVYDQDEEAASAWVDLAGIAPDRIVRRGKLDEEGELAN
                     YWHTHAAGPAGPCSEIFVDRGPRYGPDGGPDVDEERFMEIWNLVFMQEQVDGELEVVA
                     PLPGKNVDTGSSLERVAMILQGVDNVFETDLFSPTLEEAERRSGKRHGEDPNDDVSLK
                     IVAEHGRATTFLIADGVQPSNEGRGYILRRMLRRAVSHGRRLGIEGSVLDPIITTVID
                     GFGDAYPELRENEAFVRQVADSEEDRFSATLGKGLMLFEDAKGRAEGSRISGDDAFAL
                     SDTFGIPREQIQEWAAEAGLTVDTDRFAELLQEQRDRARSARKKVEVGLEAGAVPP"
BASE COUNT          404 a          755 c          777 g          336 t
ORIGIN      
        1 cagctcggcc acggtgaggg ctatcgctac cctcacgatt tcccgggtca tgcggtggac
       61 caggagtatc gccccaccac gttccagggc aagcgttact acgagccgtc gggccagggg
      121 cgtgacgtcc cccgcatccc cgggacgggc cagaaagccg aggaggagac cgaggatcgg
      181 gacgggtgag cggcatccgg gtcccccacg gcgtcgggta ggctacgcgg accaaccgca
      241 ggcgaccgac gaaaggctcg aggaacgatg acactcctgg cgatgtccgg cgacacggcc
      301 ctcacgatcg tggccgtctt ctgggccgtg ctggtccttg gcctgctggt cgtgctcctc
      361 aacaccttcc gcgtgctcga gagcacgaag atgtcgatcg acacgatgcg cgaagaggtc
      421 gtgccgctgc ttcgcgaggt caagggatcg gtggagcgcg ccaaccgcga actcgatcgg
      481 gtcgacacga tgctgatctc ggccaccgag gtcgttggtc gcgtcgagaa gctctccggc
      541 ttggccgaac aggccgccgc gagccccctc gtgaagatca tcagcgtggg tgccggtctg
      601 aagaagggct tctcgcgcgc ccggggtggc cgcaagcagt gaaacgaggg ttctgggtcg
      661 cgttgggcct cggcgccggc gccaccggcg cgatcatggc cagccgctgg accaagaagc
      721 aggcgcaacg cgtcgcgccg cagtccctgg cgcgcgaggc caaaggcggt ctgctcgact
      781 tctcgaagct ggtctccgag tcgatccaag agggcaagca ggccatggcc gagcgcgagg
      841 cccagctgcg ggccgacaac gatctgccgg tcgcaaccgg cgaacctgac gaaccgcccg
      901 ccgcctgacc caccggtaga ctccccggcg tggaaggcca ccggatccgc gagtcgttcc
      961 tgtcgttctt cgaagaacgc gatcacaagc gcgtccagag ctcgtcactg atcccaccgc
     1021 ccgagtcggg cctgctgctg acgaacgcgg gcatgaacca gttcatcccg tacttcctgg
     1081 gtcaggcatc ggcgccgtat ccgcgtgccg tcacgtcgca gaaggtgatg cgcacgaacg
     1141 acatcgagaa cgtgggccac gacgccaggc acgagacctt cttcgagatg ctcgggaact
     1201 tctcgttcgc cgactacttc aaggccgagg cgatcacgtg ggcgcacgag ctgatcaccg
     1261 aggggtacgg gatcgatcac gacctgctgt gggtcacggt ctacgaccag gacgaggagg
     1321 cggcctcggc ctgggtcgac ctggcgggta tcgcgccgga ccgtatcgtg cgtcgcggca
     1381 agctcgacga ggaaggcgag ctcgcgaact actggcacac ccacgccgcc ggccccgccg
     1441 gcccctgctc ggagatcttc gtggaccgcg ggcctcgata cggtcccgac ggcggtcccg
     1501 atgtcgacga agagcgcttc atggagatct ggaacctggt gttcatgcag gaacaggtcg
     1561 acggcgagct cgaggtcgtg gcgccgctgc cgggcaagaa cgtcgacacc ggttcctccc
     1621 tcgaacgggt ggcgatgatc ctgcagggcg tcgacaacgt gttcgagacc gacctgttca
     1681 gccccaccct cgaggaagcc gagcgacgct ccggtaagcg tcacggcgag gacccgaacg
     1741 acgacgtgtc cctcaagatc gtggccgaac acggccgcgc caccacgttc ctgatcgccg
     1801 acggggtcca gccctcgaac gaggggcgcg gatacatcct gcgccgcatg ttgcggcgcg
     1861 cggtctcaca cggtcgccgg ctggggatcg agggcagcgt gctcgatccc atcatcacca
     1921 cggtgatcga tggtttcggc gacgcctacc cggaactccg ggagaacgag gccttcgtgc
     1981 gccaggtggc cgactccgag gaagaccggt tctccgccac cctgggcaag gggctcatgc
     2041 tcttcgaaga cgccaagggc cgggcagagg gttcgcggat ctcgggcgac gatgcgttcg
     2101 cgctgtcgga cacgttcggg atcccgcgcg aacagatcca ggaatgggcg gccgaggccg
     2161 gactcacggt cgacaccgac cggttcgccg aactgctcca ggaacagcgc gaccgagcgc
     2221 gttcggcacg caagaaggtc gaggtcggtc tcgaagcggg cgccgtcccg cc
//
LOCUS       DATDBZ010000282         3364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_503618, whole genome shotgun sequence.
ACCESSION   DATDBZ010000282 DATDBZ010000000
VERSION     DATDBZ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503618"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..2070)
                     /locus_tag="VIX62_06885"
     CDS             complement(52..2070)
                     /locus_tag="VIX62_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY5859991.1"
                     /translation="MSERFRVGERRRLVVASFLMLFTELALIRWVSAYQVHVAYFTNF
                     VLLASFLGIGVGFLRSGKGRDLSRWAPAILAALVLGVFVLRVVKTNGSDALRTVFDLP
                     ALPSWVMLPLLFLGSVAAMATIAEGVARLFERFEPLEAYRLDITGSLAGIVVFSLMSF
                     FGLGPLAWGLVIAVLFIALTPREDRMRLVWCGAVVFVFALGSLAPHDTWSPYYRVTVQ
                     PADDAGRIGVVVNSLPHQSMMPLAQIQEEFYTRPYTHLRQTPQDVLIVGAGNGNDVAL
                     ALSRDVGHIDAVEIDPVIQAAGRDRHPAHPYQDPRVSPHIDDGRAYLERTDQTYDLIL
                     FALPDSLTLVSGQGSLRLESYLFTREAMETVRDHLNPGGAFAMYNYYRDDVFERYANT
                     MSEVFGHDPCFDAVDQTLGPRQQAVLTVGVEATDIDCETTFRSAVDAPEPSTDDHPFP
                     YVPDRSIPNFYLWWLGAILLASVVIVRLASGAPLGQMRPYVDLFFMGAAFLLLETKSV
                     VQFALLFGTTWFVNSLVFAGILLAVLAAIEVARRAKLPRPIVMYGALLACLAVAWLIP
                     PASLLSLSTVPRFFASVLVAFAPVFVANLIFADRFRDVGASTIAFGANLLGAMLGGVL
                     EYLAIVTGYRALLFGVAILYGLAYVTGRRHLGSREPSAPEARVGADVP"
     gene            complement(2067..3183)
                     /gene="prfB"
                     /locus_tag="VIX62_06890"
     CDS             complement(join(2067..3116,3118..3183))
                     /gene="prfB"
                     /locus_tag="VIX62_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153759895.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY5859992.1"
                     /translation="MSESTEPLRKLRERVLAAKEFLDVDGKRSQLEHLEQLASDPSLW
                     DDPAHGQAITSKLSRLKTEVDGYDRLMARIDDAVAMDELLAEETDAEMTKELSRAVSS
                     LGADVDKLELAALLSGPYDASDAIATINAGAGGTESQDWAEMLLRMYTRWAERDGFDA
                     EVDDIQHGDEAGIKSATFTVRGTNVYGLLSAERGVHRLVRISPFDAQKRRHTSFASLD
                     VIPMLEASAEEDIEIPADELRIDVYRSSGPGGQSVNTTDSAVRITHIPTGLVAACQNE
                     RSQLQNKAVAMRILKARLAELERQKREDEMDTIRGERKAVDFGSQIRSYVLAPYQMVK
                     DHRTDAEVGDPQRILDGDIDVFIEAELRHRAAEGNEG"
BASE COUNT          569 a         1135 c         1152 g          508 t
ORIGIN      
        1 ccggcgccgt ggtgtggatc gggatcctcg tggcgttcgt ggcagccgcg gtcacggaac
       61 gtcggcgccg acgcgagcct cgggcgccga cggctcccgc gatcccagat gccggcggcc
      121 cgtgacgtag gccaagccgt agaggatcgc gaccccgaac agcaacgcgc ggtaccccgt
      181 gacgatcgcg aggtactcga gcaccccacc gagcatcgcc cccaacaggt tcgcgccgaa
      241 ggcgatggtc gaggctccca cgtcccggaa ccggtccgcg aagatcaggt tcgccacgaa
      301 cacgggggcg aacgccacca gcacgctcgc gaagaaccgc ggcacggtgg acagcgacag
      361 gagcgaggcg ggcgggatca gccacgccac ggcgaggcag gccagcagcg cgccatacat
      421 cacgatcggt cgagggagct tcgctctgcg cgccacctcg atcgccgcga gcaccgccag
      481 caggatgccc gcgaacacca gcgagttcac gaaccaggtg gtgccgaaca ggagcgcgaa
      541 ctgcaccacg ctcttggtct ccagcaacag gaacgccgcg cccatgaaga acaggtcgac
      601 gtacggtcgc atctggccca acggcgcccc ggaggccaag cggacgatca cgacggaggc
      661 cagcaggatc gcgcccagcc accagaggta gaagttgggg atggagcgat cgggcacgta
      721 cgggaacggg tgatcgtcgg tggagggttc aggcgcgtcg accgcggagc ggaacgtcgt
      781 ctcgcagtcg atgtcggtgg cttccacgcc cacggtcagc accgcctgct ggcgtgggcc
      841 gagcgtctgg tcgacggcgt cgaagcacgg gtcgtgaccg aagacctcgg acatcgtgtt
      901 cgcgtagcgc tcgaacacgt cgtctcggta gtagttgtac atcgcgaacg ccccgccggg
      961 gttcagatgg tcacgcacgg tctccatggc ctcgcgcgtg aacaggtagc tctccagccg
     1021 caacgagccc tgcccgctga ccagtgtcag cgagtcgggc agcgcgaaca ggatcaggtc
     1081 ataggtctga tcggtccgct ccaggtatgc gcggccgtca tcgatatgcg ggctcacgcg
     1141 tgggtcctga tacgggtggg cgggatgccg gtctcggcct gccgcctgga tcaccgggtc
     1201 gatctcgacc gcgtcgatgt gcccgacgtc gcgggagagc gccagcgcca cgtcgttgcc
     1261 gttgcccgcc cccacgatca acacgtcctg aggggtctgc cgcaggtggg tgtacggccg
     1321 ggtgtagaac tcctcctgga tctgggcgag cggcatcatc gattggtgcg ggagcgagtt
     1381 caccaccacg ccgatccgcc cggcgtcgtc ggcgggctgg accgtgaccc ggtagtaggg
     1441 cgaccaggtg tcgtgggggg cgagagaccc cagcgcgaac acgaacacga cggcgccgca
     1501 ccacaccagc cgcatccggt cttcgcgagg cgtcaaggcg atgaagagca ccgcgatcac
     1561 gagtccccac gccaacgggc cgagcccgaa gaaggacatc aacgagaaca cgacgatccc
     1621 ggcgaggctg ccggtgatgt ccaagcggta ggcctccagc ggctcgaagc gttcgaacag
     1681 ccgcgccacc ccttcggcga tcgtcgccat cgcggccacc gaacccagga agagcagcgg
     1741 cagcatcacc cacgagggca gggcgggcag gtcgaacacc gtgcgcagcg catccgagcc
     1801 gttggtcttg accacacgca gcacgaacac cccgagcacc agggcggcca ggatcgcggg
     1861 tgcccaccgc gagaggtccc ggcccttgcc cgagcgcagg aaccccacgc cgatgcccag
     1921 gaagctggcg agcagcacga agttcgtgaa gtaggccacg tgcacctgat aggccgacac
     1981 ccagcggatc agggcgagct cggtgaagag catcaggaag ctggccacca ccagccggcg
     2041 tcgttcgccc acgcggaacc gttcgctcat ccctcgttcc cctcggcagc gcgatgacgg
     2101 agttcggcct cgatgaagac gtcgatgtct ccgtccagga tgcgctgtgg atcgccgact
     2161 tcagcatcgg tgcggtggtc cttgaccatc tgatacggag ccagcacgta ggagcggatc
     2221 tgcgagccga agtccacggc cttgcgctca ccgcggatcg tgtccatctc gtcctcccgc
     2281 ttctggcgtt cgagttccgc gagcctggcc ttcaggatcc gcatggcgac ggctttgttc
     2341 tggagttggc tgcgctcgtt ctggcaggcc gccaccaggc cggtcgggat gtgggtgatg
     2401 cgcacggccg agtccgtggt gttgaccgac tgcccgccgg ggccgctcga gcgatacacg
     2461 tcgatccgca actcgtcggc cgggatctcg atgtcctcct cggccgaggc ttcgagcatc
     2521 gggatcacat cgagcgaggc gaacgaggtg tgtcgccgct tctgcgcatc gaacgggctg
     2581 atccgcacca gacggtgcac gccgcgctcg gcgctcagca ggccgtagac gttcgtgcct
     2641 cgaaccgtga acgtcgcgga cttgatcccg gcctcgtcgc cgtgctggat gtcgtcgacc
     2701 tcggcgtcga agccgtcgcg ttcggcccac cgggtgtaca tgcgcaggag catctcagcc
     2761 caatcctggc tctcggtgcc gccggcgccg gcgttgatcg tggcgatcgc atcggacgcg
     2821 tcatacgggc cggagagcag cgccgcgagt tccagcttgt ccacgtcggc cccgagggac
     2881 gacaccgccc tcgagagctc cttcgtcatc tcggcgtcgg tttcctcggc gaggagctcg
     2941 tccatggcga cggcgtcgtc gatgcgagcc atcaaccggt cgtagccgtc gacctcggtc
     3001 ttcaggcgcg agagcttcga ggtgatggcc tgcccgtgcg cggggtcgtc ccacagcgac
     3061 gggtcggagg ccagctgttc gagatgctcg agctgcgaac gcttcccgtc gacgtcaaag
     3121 gaactccttg gcggccagga cgcgttcacg gagcttccgc aggggctcgg tggactcgct
     3181 catctggggc cagagtgtac cggccaggtt ctcggatccg gaagctggcc tcagacgacg
     3241 gcgccgtggc acttcttgta cttcttgccg ctcccgcacg ggcacggggc gttgcgcggc
     3301 gtcttgtcgc tgaccgcctg gttcgcgttg gccgtcccgg tgggagcggc gacaccgccc
     3361 gcct
//
LOCUS       DATDBZ010000283         2318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_70442,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000283 DATDBZ010000000
VERSION     DATDBZ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70442"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VIX62_06895"
     CDS             complement(<1..1338)
                     /locus_tag="VIX62_06895"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY5859993.1"
                     /translation="MSRRLDGTRGRGIDRSAPVTFTFDGHSVDGYTGDTVASALVAAG
                     IDVVCPSPILARPRGVFSAGSEEPNAFVRVSGAIVDPITPATMVTIADGLVVEGVPGV
                     GRLPGDTAGARVAEHRHVHVETLVVGAGVAGLAAIGTASSLGAVVRAFDARPEVGEQV
                     ESMGAEFLRIDVEETGPSATGYAKEMGEDFNRKAAELYAAQAADVDIIITTALIPGRP
                     APRLITEDMVASMKPGSVVVDMAASNGGNVAGTVADQKVVTDNGVTILGYTDLPGRLP
                     TQASQLFGTNLVNLLKLVTAAKDGEFALDLDDPVQRGMTVVRDGEVLWPPPPVQVSAA
                     PAAAAAPVLEPKPPKVPMSANRRLGYLGLAAAVFLLIASVSPEPFLGHFMVFMLSVVI
                     GFYVIGNVHHALHTPLMSVTNAISGIILVGALLQVGHEGEPLISVLAFVAILVA"
     gene            complement(1335..1649)
                     /locus_tag="VIX62_06900"
     CDS             complement(1335..1649)
                     /locus_tag="VIX62_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEY5859994.1"
                     /translation="MTKVEAGERHMLLIPCPWCGSRDETEFRFGGQAHLAYPADPASL
                     SDEAWADFLFMRDNPRGWWSERWMHTAGCRRWFNLQRNTATNEIGASYRMGEDPPTGV
                     TP"
     gene            complement(1627..1956)
                     /locus_tag="VIX62_06905"
     CDS             complement(1627..1956)
                     /locus_tag="VIX62_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY5859995.1"
                     /translation="MRTFALTLPEATEQETWETATFRVRNKIFCMFSGEERQLWIKSI
                     EDEQRSLIAQDAAAYFRPPYVGSKGWVGAVLAKADGDEIRELITEAWRMTAPKRLVKT
                     HDEGGSR"
     gene            complement(1991..>2318)
                     /locus_tag="VIX62_06910"
     CDS             complement(1991..>2318)
                     /locus_tag="VIX62_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015662112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5859996.1"
                     /translation="SFHVIEHQMAAAVELFPIFAHASMLRTWGGIVDVSPDASPILGP
                     TPIDGLFLNCGWGTGGFKGTPASGWAYAETIANGEAGDLAKPFALERFTTGALIDEHG
                     AAAVAH"
BASE COUNT          364 a          834 c          766 g          354 t
ORIGIN      
        1 cgccaccagg atcgccacga acgccagcac ggatatcagg ggttcgccct cgtgaccgac
       61 ctgcagcagc gcgccgacga ggatgatgcc gctgatcgcg ttggtcaccg acatcagcgg
      121 ggtgtgcagg gcgtggtgca cgttgccgat gacgtagaac ccgatgacca ccgagagcat
      181 gaagaccatg aagtgcccca ggaacggctc gggcgagacc gacgcgatca gcaggaacac
      241 cgccgccgcc aggcccaggt agcccagccg ccggttcgcg ctcatcggca ccttcggcgg
      301 cttcggctcc agcaccgggg ccgctgcggc ggccggggcc gcggacacct gcaccggcgg
      361 cggcggccac agcacctcgc catcgcgcac caccgtcata ccgcgctgca ccgggtcgtc
      421 caggtccagg gcgaactcgc cgtccttggc cgcggtgacc agcttgagca ggttgaccag
      481 gttggtgccg aacagctgcg aggcctgcgt gggcagccgg cccggcaggt cggtgtagcc
      541 caggatcgtg acgccgttgt cggtgaccac cttctggtcg gcgaccgtgc ccgcgacgtt
      601 gccgccgttc gaggcggcca tgtcgaccac gaccgagccg ggcttcatcg acgcgaccat
      661 gtcctcggtg atcagccgag gggccgggcg gcccgggatc agcgccgtgg tgatgatgat
      721 gtcgacgtcg gcggcctgcg cggcgtagag ctcggcggcc ttgcggttga agtcctcgcc
      781 catctccttg gcgtagcccg tggcgctcgg gccggtctcc tcgacgtcga tgcgcaggaa
      841 ctccgcaccc atcgactcca cctgctcgcc gacctcggga cgtgcgtcga acgcgcgcac
      901 caccgcaccc agcgaggatg ccgtgccgat cgcggccagg ccggcgacgc ccgcccccac
      961 caccaaggtc tccacgtgca cgtggcgatg ctcggcgacc cgggccccag cggtgtcacc
     1021 gggaagcctg cccacgcccg gcacgccttc gaccacgaga ccgtcagcga tcgtgaccat
     1081 cgtcgccggc gtgatcggat cgacgatggc gccgctgacc cgcacgaatg cgttcggctc
     1141 ctcggatcct gccgagaaca caccacgtgg gcgagcaagg atcggagacg gacacacgac
     1201 gtcgatgccg gcggccacca gggcgctcgc gaccgtatcg ccggtgtatc cgtcgacgct
     1261 gtgcccgtcg aaggtgaagg tgacgggcgc agaccgatcg atccctcgtc cgcgggtgcc
     1321 gtcgaggcgg cggctcacgg cgtcactccc gtgggcgggt cctcacccat ccgatacgac
     1381 gccccgatct cgttcgtggc cgtgttccgc tgcaggttga accaacggcg acacccggcg
     1441 gtgtgcatcc agcgttccga ccaccacccc cgcgggttgt cgcgcatgaa caggaagtcc
     1501 gcccaggcct catcgctcag cgaagccggg tcggcggggt aggccaggtg cgcctgcccg
     1561 ccgaagcgga actcggtctc gtcgcgcgac ccgcaccatg ggcacgggat caggagcatg
     1621 tgccgttcac cggcttccac cttcgtcatg cgtcttcacc aggcgcttgg gcgccgtcat
     1681 gcgccaagcc tcggtgatca gctctcggat ctcgtcgcca tccgccttcg cgagcacggc
     1741 ccccacccaa cccttggatc ccacgtacgg aggacggaaa taggccgccg catcttgtgc
     1801 gatcagcgaa cgctgttcgt cctcgatcga cttgatccac agctgtcgtt cctcgcccga
     1861 gaacatgcag aagatcttgt tgcgaacccg gaacgtggcg gtctcccagg tctcctgctc
     1921 ggtcgcctcg ggcaacgtca gggcgaacgt acgcacggtc gatgctcgta tccccatgag
     1981 ccagcgcccc ctagtgcgcg accgccgccg cgccgtgttc atcgatgagg gcgccggtgg
     2041 tgaaccgttc cagtgcgaac ggcttggcca ggtcgccggc ttcgccgttc gcgatcgtct
     2101 cggcgtacgc ccacccggag gcaggcgtgc ccttgaaccc cccggttccc cagccgcagt
     2161 tgaggaacag tccgtcgatc ggtgtcggcc ccaggatggg cgacgcgtcc gggctgacgt
     2221 ccacgatgcc gccccaggtt cgcagcatcg atgcgtgcgc gaagatgggg aacagttcca
     2281 cggcggccgc catctggtgc tcgatcacgt ggaaggac
//
LOCUS       DATDBZ010000284         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1099326, whole genome shotgun sequence.
ACCESSION   DATDBZ010000284 DATDBZ010000000
VERSION     DATDBZ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099326"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VIX62_06915"
     CDS             <1..640
                     /locus_tag="VIX62_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEY5859997.1"
                     /translation="VAHVPLRVGTARDEQKLYVAPARPERDDATLEAPIRQSVPPGAA
                     GRVTEVIEVYRASRQARGLGATNNDVADAVATASRFRMPALRLAEAQAAHQPRTYLYQ
                     FDWESPARRGTLGACHGLEIPFVFGSIGRTGDDRMSGTGPEADHLSGQMMDAWIAFAR
                     HGDPAHEGIGAWPGYDRDERQTMVFGRTTGVQPAPFEEERSLWESMLGSRTV"
     gene            complement(647..1048)
                     /locus_tag="VIX62_06920"
     CDS             complement(647..1048)
                     /locus_tag="VIX62_06920"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5859998.1"
                     /translation="MLTLLTEAPMHGYQIITELADRTHGVWRPSPGSIYPLLQQLADE
                     GLVVSAEQGGRNVFNLTDEGRSIATDETDGPPIWDRVAGEGTAVDLREAVDTVASAAR
                     QVARTGTTEQATKAAEILTEARKRLYQLLAE"
     gene            1363..>2265
                     /locus_tag="VIX62_06925"
     CDS             1363..>2265
                     /locus_tag="VIX62_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEY5859999.1"
                     /translation="MTDTDADDDGATRVVLSRVARLRQRAMALVDQAMELYEHTRATI
                     PPVDIAATAVERDNRIGGFLLAGAIAFRFFVYLLPLYLLVLLAAGAALSFDPDSPAEL
                     GESAGLSQYLASTIGDAAQTSKDSLWILAPVTLWALLTAGRSAHKVLAVSHANAWGVS
                     PPRGLKPHKVSAMIFVFALAMLAGIVTFRNLHTGYWAPVGFGGAAIYYFFLWLAASRM
                     LPRSPEAPTAALIPGAVLVAAGSQGLYLFNALYLNQKIQSSSEAYGALGVAASLLLWL
                     YLMGRLMVAAPVLNATMWERSRAEE"
BASE COUNT          305 a          795 c          776 g          389 t
ORIGIN      
        1 cgtcgcccac gtcccgctca gggtggggac cgcacgcgac gagcagaagc tgtacgtggc
       61 cccagctcgg ccagagaggg acgacgcaac gctcgaggcg ccgatccgcc agagcgttcc
      121 gccgggcgcc gctggtcggg tgaccgaggt gatcgaggtg taccgcgcat cccgccaggc
      181 acgcggcctc ggggcgacga acaacgacgt cgccgatgcg gtggccaccg cctcccggtt
      241 ccgaatgccc gcgctacggc tcgccgaggc acaggctgcc caccaaccgc ggacctacct
      301 ctaccagttc gactgggagt caccggcccg ccgggggacg ctcggcgcct gtcacggcct
      361 cgagatcccc ttcgtgttcg gcagcatcgg ccgcaccggc gacgatcgca tgagcggcac
      421 cgggcccgag gccgaccacc tctcgggtca gatgatggac gcgtggatcg cgttcgcccg
      481 ccacggtgac ccggcccacg aggggatcgg tgcgtggccc ggctatgacc gcgacgagcg
      541 ccagacgatg gtcttcggcc gcacgaccgg cgtgcagccg gccccgttcg aggaggaacg
      601 ctcgctctgg gagtcgatgc tcgggagccg caccgtctga tcctgcctac tcggccagga
      661 gctggtacag ccgcttgcgg gcctcggtca ggatctccgc tgccttggtg gcctgctcgg
      721 tcgtgcctgt cctggcgacc tgacgggcgg ccgacgccac ggtgtcgacg gcctcgcgga
      781 gatcgaccgc cgtcccctcg ccggcaacgc gatcccagat cgggggtccg tcggtctcgt
      841 cggtggcgat ggagcgacct tcgtcggtga ggttgaagac gttgcggccg ccttgctcgg
      901 ccgagacgac cagtccctcg tcggcgagtt gctggagcag tggatagatc gacccggggc
      961 tcggccgcca cacgccgtgg gtgcggtcgg cgagctcggt gatgatctgg tagccgtgca
     1021 tcggcgcttc ggtgaggagg gtgagcacgg cgatgcggac gtcgccgcgg gcgacgccgc
     1081 ggccgcggcc acgaccagcg cctcggaccc cgctccgtcc acggcccgat ccaggaccgc
     1141 gtcgacctcc cggtccgcga ctgctggttg gtccactcat ggtcatgatc cattctccac
     1201 gcgttcgtgg gtgtccttct ctgtgctcgg tcatcggtgt gtccttcgtg ttggcgacgg
     1261 tcattccgtc gatgtgatca cgatatatcg cgagatgatc ggctgcaacc ctcgactgca
     1321 accctgcgat cgccggtgcg gaacctgcga gatgatgtgc ccgtgaccga cacggacgcc
     1381 gacgacgacg gggcgactcg ggtcgtgctc tctcgcgtcg cgcggctccg gcaacgggcc
     1441 atggcgctgg tcgaccaggc catggagctc tacgaacaca cgagagccac gatcccaccg
     1501 gtggacatcg cggccacggc ggttgagcgc gacaaccgca tcggagggtt cctcctcgcc
     1561 ggggcgatcg cctttcgctt cttcgtctac ctcctcccgc tctacctgct ggtccttctg
     1621 gcggcgggtg ccgctctgtc gttcgacccc gacagccctg ccgagctcgg tgagagcgct
     1681 ggactgtcgc agtacctggc gagcacgatc ggcgacgccg cccagacctc gaaggacagc
     1741 ctctggatcc tcgctcccgt gaccctgtgg gcgctgttga ccgcgggccg ttcggcgcac
     1801 aaggtgctcg cggtctccca cgccaacgcg tggggtgtgt cgcccccgcg tgggctcaag
     1861 cctcacaagg tgagcgccat gatcttcgtg ttcgccctgg cgatgttggc gggcatcgtc
     1921 accttcagga acctccacac cgggtactgg gctccggtgg gcttcggtgg cgcggcgatc
     1981 tactacttct tcctgtggct cgcggcgtcc cggatgctgc cgcgctctcc ggaagcgccg
     2041 actgcggcac tcatccccgg ggcggtcctc gtcgcggccg gctcgcaggg tctctacctg
     2101 ttcaacgccc tgtacctgaa ccagaagatc cagtcgtcgt cggaggccta cggcgcgctc
     2161 ggggtggccg cgtcgctgtt gctgtggctc tacctgatgg ggcggctgat ggtcgccgct
     2221 cccgttctca acgccaccat gtgggagcgc tcgcgtgcgg aagag
//
LOCUS       DATDBZ010000285         3770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1532598, whole genome shotgun sequence.
ACCESSION   DATDBZ010000285 DATDBZ010000000
VERSION     DATDBZ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532598"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(481..2685)
                     /locus_tag="VIX62_06930"
     CDS             complement(481..2685)
                     /locus_tag="VIX62_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY5860000.1"
                     /translation="MSERRRSHGLLAAIVGGLIIGAVEAVLATAFAAFVYRGLLHQRL
                     PDAIGLYLGAAALTLAVFAWRAGPRGVVASVQDAAAAVLAVVAGITAAKAAQLDIVAR
                     AAGLKDYERPDVYLTVVATTLVVTVLCGVVFLVLGWRRLGNFIRFVPYPVMGGFLAGT
                     GWLLFKGGIEASTGVVVHLRTISDLTRQYDLVRLLPALAFGVILLLAVRIVKRPIVIP
                     AVIALGLVAFVAVALATGSSVETIREGGWLLGPFETTRLWQPWTLRALSGADWLAVLE
                     SWPVILTAVFVATLALLFNVSGTEIVLDRDLDTNEELRDSGVVNIISGALGGIPGYHA
                     LSLTSLAERMSVDARAAGLVAALVPLGAVVFGAPVIELIPRMIVGGVLVFVGLAFIVE
                     WVWDKRRSLPPIEYGVVLVILAVIIARGFLPGVVVGLVLAVVLFAISYGRVELVREVA
                     FGETYRSNVDRPAAERAQLRTLGEQVQILRVNGFVFFGSTNGLLERIRHRIEAEPLAF
                     LVIDLQRVTGVDSSAVAAFAKAMRLAEGGGFEIVFTGPSDSVRAQLERGGVVPTEGTV
                     GFEPDLDRGLQRCEDALLAAETPDHAAAGADQVEGLGDLPPGLSDYLERVSVPAGTVL
                     LHQGDPPGDVYVLESGHLGVETQTPEGPRIRLRSIRPGVVIGEIAFYTAVPRTADVVA
                     ETDSVVLRFSAPSIRRLETEQPEVAAEFHRWLATALADRLSDTMRSVGALLR"
     gene            complement(2744..>3770)
                     /locus_tag="VIX62_06935"
     CDS             complement(2744..>3770)
                     /locus_tag="VIX62_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5860001.1"
                     /translation="DKRRSLPPIEYGVVLVILAVIIARGFLPGVVVGLVLAVVLFAIS
                     YGRVELVREVAFGETYRSNVDRPAAERAELRALGDQVQILRVNGCVFFGSTNGLLERI
                     RHRIEAGPLAFLVIDLQRVTGMDSSAVAAFAKAMRLAEGGGFEIVFTGPSDSVRARLE
                     RGGVVPTEGTVGFEPDLDRGLQRCEDALLAAETPEHAAAGADQTEGLGDLPPGLSGYL
                     ERVSVPAGTVLLHQGDPPGDVFVLESGHLGVETQTPEGLRIRLRSIRPGVVIGEIAFY
                     TAAPRTADVVAETDSVVLRFSAESIRRLEAEQPELAAEFHRWLATALADRLSDTMRTF
                     DALVDQT"
BASE COUNT          687 a         1337 c         1254 g          492 t
ORIGIN      
        1 ggcggccgcg ggatcgtcct cgaacacccg ggctctgacg atcgtcccgt ccgagtactc
       61 gtacacgacc acccgcatcc acgcgccgtc gccggcatca gacccgatcc tcccgagcag
      121 gagacgtggc gatcgcccgc atccaggtcg aggaactccg gcttgaaccg actggctgag
      181 tagcggtctt ggttcgccaa gaagcagagg agtgccgtcc cggccccgat actcaccatg
      241 gagcgggccg cgaccggggt aactgaagac ggcgtcctcg gcgaagaacc gtgccccgca
      301 tctcgatcgt cgcggcccca gccttcgagg agttgccgga agagcgctta gttcgggtgc
      361 gccgtgcgga tcactgcttc gagggatcct ccctccagca tgcgagagtg atatgacgaa
      421 gccgcgatct cggtcaagcg atcgatcagg ccggagggga cgactccgat ccacagcgcg
      481 ctaacggagg agcgcgccca ctgacctcat cgtgtcgctc aagcgatcgg ccagcgcggt
      541 cgcgagccag cggtgaaact cggcggcgac ctcgggctgt tcggtctcga gccttcgaat
      601 cgacggggcg gagaagcgaa ggaccacgct gtcggtctcg gccaccacat cggctgtgcg
      661 cgggaccgcg gtgtagaagg cgatctcgcc gatcacgacg ccgggacgga tcgaacgcag
      721 gcggatccgc gggccctcgg gggtttgcgt ctccacgccc aggtgcccgg actcgagcac
      781 gtagacgtca cccggcggat cgccttggtg gagcaacacc gtgcccgcgg ggacagagac
      841 gcgctcgagg tagtcggaaa gcccgggggg cagatcgccg agaccctcga cctgatcggc
      901 gcctgcggcc gcgtgatccg gggtctcggc cgcgagcagc gcatcctcac accgttgcag
      961 cccacggtcc aggtcgggtt cgaagccgac ggtcccctcg gtggggacca cccctccgcg
     1021 ctcgagctgt gcgcgcaccg agtcagaggg acccgtgaag acgatctcga agccgccccc
     1081 ctccgccagt cgcatcgcct tggcgaacgc ggctaccgcc gaggagtcca cgcctgtgac
     1141 ccgctgcaga tcgatcacca agaacgcaag cggctccgct tcgatgcgat gtcggatccg
     1201 ctcgagcagg ccgttggtcg aaccgaagaa cacgaacccg ttcacccgca ggatctggac
     1261 ctgctccccg agcgtgcgca gctgggcgcg ttcggccgcc ggccggtcga cgttgctgcg
     1321 gtaggtctct ccgaacgcga cctcgcgcac gagctccacc cgcccgtagc tgatcgcgaa
     1381 caggaccacg gcgagcacga gcccgacgac gacccccggc aagaaccccc gggcgatgat
     1441 caccgcgagg atcaccagaa cgaccccgta ttcgatcggc ggcaaggacc gccgcttgtc
     1501 ccagacccac tcgacgatga aggcgagacc cacgaacacg agcaccccac ccacgatcat
     1561 ccgcgggatc agctcgatga cgggggcacc gaacacgacg gcgcccagag ggaccagcgc
     1621 ggccaccagc cccgcggctc gggcgtcgac gctcatgcgc tcggcgagcg aggtcagaga
     1681 cagcgcatgg tatccgggga tcccgccgag cgcaccagag atgatgttca cgacccctga
     1741 gtcgcgcagc tcctcgttcg tatcgaggtc gcgatccaag acgatctcgg tcccgctgac
     1801 gttgaacaac agcgcgaggg tcgcaacgaa cacggcggtg aggatcacgg gccaggattc
     1861 gagcacggcc aaccagtcgg caccggagag cgcgcgaagt gtccacggtt gccacagccg
     1921 tgtcgtctcg aaaggtccga gcagccaccc tccctcgcgg atggtctcga ccgaagagcc
     1981 ggtggcgagc gcgacggcaa cgaacgccac cagtccgagc gcgatcaccg cggggatgac
     2041 gatgggccgt ttcacgatcc ggacggcgag cagcaagatc accccgaacg ccaacgccgg
     2101 gagcaaccgg accagatcgt attggcgggt caggtcgctg atcgtccgca gatgcaccac
     2161 gacgccggtc gacgcctcga tcccaccctt gaacagcagc catcccgtgc cggcgaggaa
     2221 ccctcccatg accgggtagg gcacgaaccg gatgaagttg ccgagccggc gccacccgag
     2281 caccaggaac acgactccgc acaggaccgt caccaccagg gttgtggcca cgacggtgag
     2341 gtagacgtcg gggcgctcgt agtccttcaa gcccgcggcc ctggccacga tgtcgagctg
     2401 agcggccttg gccgccgtga tgccggccac gaccgcgagc acggccgcgg cggcatcctg
     2461 gacgctcgcc acgacccccc tgggaccagc tcgccacgcg aagaccgcga gcgtgagggc
     2521 ggcggccccc aggtacaggc cgatggcgtc gggaagccgt tggtggagca gaccgcgata
     2581 cacgaacgcc gcgaacgcgg tggcgagcac ggcctcgacg gccccgatga tcaacccgcc
     2641 gacgatcgcg gcaaggagcc cgtgagatcg tcgccgttca gacaccgtcg cccccgtgca
     2701 cctcgcgagt cgcgcgcacc ctaggcatcc ccatccgtgg agttcatgtc tggtcgacca
     2761 aggcgtcgaa cgtcctcatc gtgtcgctca agcgatcggc cagagcggtc gcgagccagc
     2821 gatggaactc ggcggcgagc tcgggctgct cggcctcgag ccttcggatc gactcggcgg
     2881 agaagcgaag gaccacgctg tcggtctcgg ccaccacatc ggccgtgcgc ggggccgcgg
     2941 tgtagaaggc gatctcgccg atcacgacgc cggggcggat cgagcgcagc cggatccgta
     3001 ggccctctgg ggtttgggtc tccacgccca ggtgcccgga ctcgagcacg aagacgtcac
     3061 ccggtgggtc gccttggtgg agcaacaccg tgcctgcggg gacggagacg cgctcgaggt
     3121 agccggaaag gccggggggc agatcgccga gaccctcggt ctgatcggcg cctgcggccg
     3181 cgtgctctgg ggtctcggcc gcgagcagcg catcctcaca tcgctgcagc ccacggtcca
     3241 gatcgggttc gaagccgacg gtcccctcgg tggggaccac ccctccgcgc tcgagccgtg
     3301 cgcgcaccga gtcggagggg cccgtgaaga cgatctcgaa gccgcccccc tccgccagtc
     3361 gcatcgcctt ggcgaacgca gccactgccg aggagtccat gccggtgacc cgctgcaggt
     3421 cgatcaccaa gaacgcgagc ggccctgcct cgatgcgatg tcggatccgc tcgagcaggc
     3481 cgttggtcga accgaagaac acgcacccgt tcacccgcag gatctggacc tgatccccga
     3541 gcgcgcgcag ctcggcgcgt tcggccgccg gccggtcgac gttgctgcgg taggtctctc
     3601 cgaacgcgac ctcgcgcacg agctccaccc gcccgtagct gatcgcgaac agaaccacgg
     3661 cgagcacgag cccgacgacg accccgggca agaacccccg ggcgatgatc accgcgagga
     3721 tcaccagaac gaccccgtat tcgatcggcg gcaaggaccg gcgcttgtcc
//
LOCUS       DATDBZ010000286         3303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_304675, whole genome shotgun sequence.
ACCESSION   DATDBZ010000286 DATDBZ010000000
VERSION     DATDBZ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304675"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="VIX62_06940"
     CDS             complement(<1..1737)
                     /locus_tag="VIX62_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY5860002.1"
                     /translation="MPRARPGPAVRLVLASCLMLFVELGLIRYSGAYVVYLSFFTNFV
                     LLASFLGVGVGFLRARSSPDLTRWAPFLLLALTLFIALFPVSAGRIDGQRVLQGSFGL
                     SPLPIWVSLPLIFALSFAVMASIAQGVADLFARFEPLEAYRLDIAGSVLGILAFSAIS
                     FVHAPPLWWGVAVVVLGIAVDRGGPSPVRLGAAVAMVAVLGVLSFAPDTWWSPYYRVH
                     LGDQAGPGSVDLRVNGLPHQEITPIDVLRSEQPFRYDAYDHDPDNALDDVLIVGSGSG
                     NDVAIALSKGAGHVDAVEIDPVIQQLGQERHPDRPYGDPRVDVHIQDGRAFLHDDERR
                     YDLILFALPDSHTLISGQASLRLESYLFTEEAMDEAYEHLRPDGTLAMYHFYTPTVID
                     RYAGTLDSVFGHPPCIDKRTQVGVRYQTVLTINVQPDGLDCTTPWVASGPVPAPDSDD
                     HPFPYLVRRTIPLQYVVSLAIILLASLAVVRVAAGPLRQMSAYLDLFFMGAAFLLLET
                     KNVVQFALLFGTTWFVNAFVFIGVLLSVLLAVAVSKRVVFARAWPLYVLLLATVALNW
                     AIPGRALLALPTP"
     gene            1907..3277
                     /locus_tag="VIX62_06945"
     CDS             1907..3277
                     /locus_tag="VIX62_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5860003.1"
                     /translation="MAARVEFDLDLIDSLIEREEATLVPSRSASIAYREVADRHVAGG
                     VASSWQDSPPHAIYIDRGSRNHIWDIDQNEYIDFHLGYGAMVAGHAHPKIVEAIQRQA
                     ERGTHFAQPTKHLDVIGENLADRFRLPLWRFCNSGTEATLEAGRLMRANTGRDMLVKI
                     EGTYHGHHDSLMFSVAPDPADMGPREHPVTVHQAMGIPQAFADLVRVVPFNDLEAARR
                     VFAEHEGKIAGMIVEPAMMNCGVILPEPGYLQGLKDLCHANGAYLAFDEVKTGATLAY
                     GGAVEAFGVVPDIVCLAKAIGGGTPCGAIGATRELYQPIVDGTYDMAGTFNGNPLTMA
                     ASQATLLEVLVPDAYEGFNRIDKAMKDGLTSVIETTGLPAYVSGVGAKGSVIYSTEPV
                     REYRDAVGIDERISYLAWLFQQNRGVFKSPWTKQETWTLSVWHTEDDAARYVENFEEF
                     ATAVTA"
BASE COUNT          599 a         1111 c         1144 g          449 t
ORIGIN      
        1 cggcgtgggc agcgccagca gtgcccgacc ggggatcgcc cagttcagtg cgacggtcgc
       61 taggagcaag acgtacaacg gccacgcgcg ggcgaacacg acgcgtttgc tgacggcgac
      121 ggcgagcagc accgacagca gcaccccgat gaacacgaag gcgttgacga accacgtcgt
      181 gccgaacagc agcgcgaact gcacgacgtt cttcgtctcg agcagcagga atgccgcacc
      241 catgaagaac aggtcgaggt aggccgacat ctggcgcagc gggccggcag ccacccgaac
      301 caccgccagt gacgccagca ggattatggc gaggctcacg acgtactgca gcgggatcgt
      361 tcgccgaacg aggtatggga acggatgatc gtcggagtcg ggtgccggca ccggcccgct
      421 cgcgacccac ggcgtggtgc agtcgagccc gtcgggttgc acgttgatcg tgagcaccgt
      481 ctggtaccgc accccgacct gggtgcgctt gtcgatgcag ggcgggtggc cgaagaccga
      541 gtcgagggtg ccggcgtatc ggtcgatcac ggtcggggtg tagaagtggt acatcgcgag
      601 ggtgccgtcg ggccgcagat gttcgtacgc ctcgtccatc gcttcctcgg tgaacaggta
      661 gctctcgagg cgcagcgacg cttgccccga gatgagcgtg tgcgagtccg gcagcgcgaa
      721 caggatcagg tcgtagcgtc gctcgtcgtc gtgcaggaac gccctgccgt cctggatgtg
      781 cacgtcgacc cgtgggtcgc cgtagggacg gtccgggtgc cgttcctgcc cgagttgctg
      841 gatcacggga tcgatctcca cggcgtcgac atggccggcg cccttcgaga gcgcgatcgc
      901 gacatcgttg ccgcttcccg acccgacgat caacacgtcg tcgagcgcgt tgtcggggtc
      961 gtggtcgtag gcgtcgtacc ggaagggctg ctccgaccgc agcacgtcga tcggcgtgat
     1021 ctcctggtgg gggagcccgt tcacgcgcag gtcgacggag ccgggtccgg cctgatcgcc
     1081 caggtgcacc cggtagtacg gcgaccacca ggtgtcgggc gcgaacgaga gcacgccgag
     1141 caccgccacc atcgcgacgg cggcaccgag ccgcaccggt gacggcccgc cacggtcgac
     1201 cgcgatgccg agcacgacga cggccacccc ccaccacagt ggcggcgcgt gcacgaacga
     1261 gatggctgag aacgcgagga tgccgagcac gctgccggcg atgtcgagcc ggtaggcctc
     1321 gaggggttcg aaccgggcga acaggtcggc gacgccctgc gcgatggacg ccatcacggc
     1381 gaacgacaac gcgaagatca ggggcagcga gacccagatc ggcagcgggg acagtccgaa
     1441 cgagccctgg agcacgcgct ggccatcgat gcggccggcg ctcaccggga acagcgcgat
     1501 gaacagcgtg agcgcgagca gcaggaacgg cgcccaacgt gtcaggtccg gactcgaccg
     1561 ggctcgcagg aaccccacac cgacgcccag gaagctcgca agcagcacga agttcgtgaa
     1621 gaacgacagg tagacgacgt aggcgccgga gtagcggatc aggccgagtt ccacgaacag
     1681 catcaggcac gatgcgagca cgagacgcac cgcaggaccg gggcgtgctc gcggcatcgg
     1741 tgcggcgctc gtcgcatcga ctcgggtcgt cagagcgggc atcgtctcac gcccgtctac
     1801 ccacgtcccc ggcgctgggg tatcatcagc ggacgtttga accgccgttc aatctcgtcg
     1861 ccctcgcggg cggctacggc ggttcctcga aggaggagat cgcaccatgg cagcacgggt
     1921 cgagttcgat ctggacctga tcgacagcct gatcgagcga gaggaagcca cgctcgtccc
     1981 ctcgcgctcg gcatcgatcg cctaccgcga ggtcgccgat cgccacgtgg ccggcggggt
     2041 cgcctcgagt tggcaggact cgcccccgca cgcgatctac atcgaccgcg gcagccgcaa
     2101 ccacatctgg gacatcgacc agaacgagta catcgacttc cacctcggct acggcgcgat
     2161 ggtggcgggc cacgcgcatc cgaagatcgt cgaggcgatc cagcgacagg ccgaacgagg
     2221 cacgcacttc gcccaaccca cgaagcacct cgacgtcatc ggggagaacc tcgccgaccg
     2281 ctttcggctg cccctgtggc gtttctgcaa ctcgggcacc gaggcgacgc tcgaggccgg
     2341 gcggctgatg cgcgcgaaca ccgggcgcga catgctcgtg aagatcgagg gcacctacca
     2401 cggccaccac gactccctga tgttctcggt ggcgcccgac cccgccgaca tggggccgcg
     2461 cgagcacccg gtcacggtgc accaggcgat gggcatcccg caggcgttcg ccgacctcgt
     2521 gcgggtcgtg ccgttcaacg acctcgaggc cgcccggcgc gtgttcgccg agcacgaagg
     2581 caagatcgcc ggcatgatcg tggagcccgc gatgatgaac tgcggcgtga tcctgcccga
     2641 gccgggctac ctgcaggggc tcaaggacct ctgccacgcc aacggcgcct acctcgcctt
     2701 cgacgaggtg aagacgggtg ccacgctggc ctacggcggc gcggtcgagg ccttcggcgt
     2761 cgtgccggac atcgtgtgtc tcgccaaggc gatcggtggc ggcaccccgt gcggcgcgat
     2821 cggcgcgacg cgcgagctct accagcccat cgtcgacggc acctacgaca tggccggcac
     2881 gttcaacggc aacccgctca cgatggccgc ctcgcaggcg acgctgctcg aggtgctcgt
     2941 gcccgacgcc tacgagggct tcaaccgcat cgacaaggcg atgaaggacg gcctcacgtc
     3001 ggtgatcgag acgaccgggc tgcccgcgta cgtgagcggg gtcggcgcca aggggtcggt
     3061 catctactcg accgagcccg tgcgcgagta ccgcgacgcc gtcggcatcg acgagcgcat
     3121 cagctacctc gcctggctgt tccagcagaa ccgcggcgtg ttcaagagtc cctggaccaa
     3181 gcaggaaacc tggacgctgt cggtgtggca caccgaggac gacgccgcgc gctacgtgga
     3241 gaacttcgag gagttcgcga ccgccgtcac cgcgtagcct gaggcagacc acgaacgatg
     3301 ggg
//
LOCUS       DATDBZ010000287         3483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2755943, whole genome shotgun sequence.
ACCESSION   DATDBZ010000287 DATDBZ010000000
VERSION     DATDBZ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2755943"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VIX62_06950"
     CDS             <1..737
                     /locus_tag="VIX62_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY5860004.1"
                     /translation="GRDGNSHIGDWDGNRTFAVISHLPIAYPVACGAATAYQRAGDGR
                     VAMAICGDGSTSNGRWHESLNWSAVHQLPVVWIVNNNQYAYSTPNPLEFHVPTIAERA
                     VAYGMPGVRVDGGDVLEVYEAALAAVQRAREGGGPTLIESVSLRWRGHAGHDPASYMP
                     AELLEQYMAEKDPVKNFEEWMLAEGVVTKEDIEAIAERIAADFEAGYEFAQASPFPEA
                     GDVTKGLWAEDGYWDAEPGRGGGTGP"
     gene            737..1798
                     /locus_tag="VIX62_06955"
     CDS             737..1798
                     /locus_tag="VIX62_06955"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY5860005.1"
                     /translation="MAKTAQVAKGLDGAAAGHGAEPRGKRTDDGLATYLIAIAEGLWE
                     EMERDERVYLLGEDIGVYGGAFKVTEGFVDHFGTQRVMDTPIAEETIVGVAVGSAMEG
                     LRPVAEFQYADFMSSGFDEITTVLARYHYRTGVRLPVVLRAPSGGGVRASNFHSTNPE
                     PWFAHAPGLKVVCPSSPTDAKGLIKSAVRDDNPVVYLEHKWLYRRFKESVPEDPDFLV
                     PIGKADVKREGTDLTIVTYGAMVYRALEAADNLAAKGASIEVVDLRSIVPMDEETVLE
                     SIAKTSRALVLYESHRFLGVGAEVAAMIAEKAFEHLDAPVERLAPPNTPVPFSPPLED
                     AYLPQVSDIEAAVERISAW"
     gene            1737..3188
                     /gene="sucB"
                     /locus_tag="VIX62_06960"
     CDS             1737..3188
                     /gene="sucB"
                     /locus_tag="VIX62_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEY5860006.1"
                     /translation="MPTCRRYPTSRPRSSGSLHGDHRQAEPGGDAPVTSIQMPQLGET
                     IIEGTILKWLKQEGDTIERDEPLFEISTDKVDTEVPSPVAGTVTKILVPEGETVAVGT
                     ELAEIDSGDGAAAATATPAAAAEDSAEDPAAEVPAVSAAEPVPAPQAARPAAQAPAAA
                     ATTAPAMQDRGPRSQILSPLVRKLAAEHHLDLSSIAGTGTGGRITKHDVEAAIAAGPA
                     AVPTSGVPSPGAASQPAPSVTPVAPSAPSTPGQGEEIVPLTHIRKAIGEHMVASLATS
                     ARAWTMVEVNVDHLVKLRERVKESFLERYGVKLTYLPMVTRAVSEALLSFPMVNAELR
                     GDELVVRKYVNVGIAVSYDQGLIVPVVRNVDAMNVPGIARAIADLAARARSHQLKPDE
                     VTGATFTITNPGPYGSLLSVPIINQPNAGILSLDTIQKRPVVIDDAIAIRSMVYLSMS
                     WDHRVIDGELATRFLARVKQNLESWDFAEDLGV"
     gene            3199..>3483
                     /locus_tag="VIX62_06965"
     CDS             3199..>3483
                     /locus_tag="VIX62_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013126171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase"
                     /protein_id="HEY5860007.1"
                     /translation="MTRPTWLIEPAGPVPYATANDAMHDLAQRRLDGGVPDSLILLEH
                     PPVFTAGRRSKPEHLVWSEDEAVAHGAEVHHIDRGGSFTFHGPGQLVGYPI"
BASE COUNT          582 a         1242 c         1169 g          490 t
ORIGIN      
        1 ggggacggga cggcaacagc cacatcggcg actgggacgg caaccgcacg ttcgcggtga
       61 tctcgcatct gccgatcgcc tacccggtgg cctgcggcgc cgccaccgcc tatcagcggg
      121 ccggtgacgg ccgggtggcg atggcgatct gcggcgacgg ttccacctcg aacgggcgtt
      181 ggcacgaatc gctcaactgg tcggccgtcc accagctgcc ggtcgtgtgg atcgtgaaca
      241 acaaccagta cgcctactcc accccgaacc cgctggagtt ccacgtcccc acgatcgccg
      301 agcgcgccgt cgcctacggc atgcctggcg tccgggtcga cggcggcgac gtcctcgagg
      361 tctacgaggc cgccctcgcc gccgtgcaac gtgcccgcga aggaggcggc cccacgctga
      421 tcgaatcggt ctcgctccgc tggcgcggcc acgcgggcca cgacccggcc agctacatgc
      481 ccgccgaact gctcgagcag tacatggccg agaaggaccc ggtgaagaac ttcgaggaat
      541 ggatgctcgc cgagggcgtc gtcaccaagg aggacatcga ggccatcgcc gagcgcatcg
      601 ccgccgactt cgaggccggc tacgagttcg cgcaggcgtc gcccttcccc gaggcgggcg
      661 acgtgaccaa gggcctgtgg gccgaggacg gctactggga cgccgagccc ggccgcggcg
      721 gcgggacggg accgtgatgg ccaagaccgc gcaggttgcc aaaggactgg atggggccgc
      781 agcaggtcac ggggccgagc cccgaggcaa gcgcaccgac gacggtctcg ccacctacct
      841 catcgccatc gccgagggct tgtgggagga gatggagcgc gacgagcgcg tctacctgct
      901 cggcgaagac atcggcgtgt acggcggcgc cttcaaggtg accgaagggt tcgtcgacca
      961 cttcggaacg cagcgtgtga tggacacacc gatcgccgaa gagacgatcg tgggtgtggc
     1021 ggtgggttcg gcgatggagg gcctgcgtcc ggtcgccgaa ttccagtacg cggacttcat
     1081 gtcgagtggg ttcgacgaga tcaccaccgt gctggcgcgc taccactacc ggaccggcgt
     1141 gcgtctgccc gtggtgctgc gcgcgccgag cggcggcggc gtgcgcgcct cgaacttcca
     1201 ctccaccaac cccgagccct ggttcgcgca cgcgccgggc ctcaaggtgg tgtgcccgtc
     1261 ctcgcccacc gacgccaagg ggctgatcaa gtcggcggtg cgcgacgaca acccggtcgt
     1321 ctacctcgag cacaagtggc tgtatcggcg tttcaaggaa tcggtgcccg aggacccgga
     1381 cttcctggtc ccgatcggca aggccgacgt gaagcgcgaa ggcaccgacc tcacgatcgt
     1441 gacctacggc gccatggtgt accgcgcgct ggaggccgcg gacaacctcg ccgccaaggg
     1501 cgcctcgatc gaggtcgtcg acctccgctc gatcgtcccg atggatgagg agaccgtgct
     1561 cgagtcgatc gccaagacct cgcgcgcgct ggtgctgtac gagtcgcacc ggttcctggg
     1621 ggtgggcgcc gaggtggcgg ccatgatcgc cgagaaggcg ttcgaacatc tcgacgcgcc
     1681 ggtggagcgc ctcgctcccc cgaacacgcc cgtgcccttc tctcccccgc tggaagatgc
     1741 ctacctgccg caggtatccg acatcgaggc cgcggtcgag cggatctctg catggtgacc
     1801 accgacaggc cgaacccgga ggcgatgcac ccgtgacctc gatccagatg ccacagctcg
     1861 gcgagacgat catcgaaggc acgatcctga aatggctcaa gcaggagggc gacacgatcg
     1921 agcgcgatga gccgctcttc gagatctcca ctgacaaggt ggacaccgag gtcccctccc
     1981 ccgtcgccgg caccgtgacg aagatcctcg tgcccgaagg agaaaccgtc gccgtcggca
     2041 ccgagctcgc ggagatcgac tcgggcgacg gcgcggccgc tgccacggcc acccctgcag
     2101 ccgccgctga agactccgcc gaagaccccg cagccgaggt ccccgcggtc tccgccgccg
     2161 agccggtccc cgcgcctcaa gccgctcggc ccgcggcgca agccccggcg gcggcagcaa
     2221 cgacggcccc cgcgatgcag gaccggggcc cccgctccca gatcctctcg ccgttggtcc
     2281 gcaagctcgc cgccgaacac cacctggatc tgtcgtcgat cgccgggacg ggcaccggcg
     2341 gccgcatcac caaacacgac gtcgaagccg ccatcgccgc cgggccggct gcggtgccga
     2401 catcgggcgt tccttcccca ggggcggcat cgcagccggc cccgagcgtg accccggttg
     2461 caccgtccgc gccctcaacg ccgggccaag gcgaggagat cgtgccgctc acgcacatcc
     2521 gcaaggcgat cggcgagcac atggtcgcct cgctcgccac ctcggcccgc gcctggacga
     2581 tggtcgaggt caacgtcgac cacctcgtga agctccgcga gcgcgtgaag gagtccttcc
     2641 tcgagcgcta cggcgtgaag ctcacctacc tgcccatggt cacgcgcgcg gtgtccgagg
     2701 cgctgttgtc gttcccgatg gtcaacgccg agcttcgagg ggacgaactc gtcgtccgca
     2761 agtacgtgaa cgtggggatc gccgtgagct acgaccaagg actgatcgtg ccggtggtga
     2821 ggaacgtcga cgcgatgaac gtccccggga tcgcgcgagc catcgccgac ctggcggcgc
     2881 gcgcacggtc gcatcaactc aagcccgacg aggtgaccgg cgccacgttc acgatcacga
     2941 accctggtcc gtacggatcc ctgctgagcg tgccgatcat caaccagccc aacgccggga
     3001 tcctctcgct cgacacgatc cagaagcggc cggtcgtgat cgacgacgcg atcgcgatcc
     3061 gctcgatggt gtacctgtcg atgtcgtggg atcaccgggt catcgacggc gaactcgcga
     3121 cccggttcct ggcccgcgtc aagcagaacc tcgagagctg ggacttcgcc gaggacctcg
     3181 gggtctgatc gaccggccat gacgcgtccc acgtggctga tcgaacccgc cgggccggtg
     3241 ccgtatgcga cggccaatga cgcgatgcac gatctggcgc agcggcggct ggatggcggg
     3301 gtgcccgatt cgctgatcct gctggagcac ccgccggtgt tcaccgcggg ccggcgctcc
     3361 aagcccgagc acctggtgtg gagcgaggac gaagccgtcg cccacggcgc cgaggtccac
     3421 cacatcgatc gcggcggttc cttcaccttc cacggaccgg gccaactcgt cggctatccg
     3481 atc
//
LOCUS       DATDBZ010000288         5733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_714986, whole genome shotgun sequence.
ACCESSION   DATDBZ010000288 DATDBZ010000000
VERSION     DATDBZ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714986"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VIX62_06970"
     CDS             <1..1047
                     /locus_tag="VIX62_06970"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEY5860008.1"
                     /translation="HGSIELYRAIFRHYTGLTWDQVRGRAGAFVEPIDALDVQLLPEM
                     EGIAEGAAVDTEDVLALNVRTEVMYGLGAPGAAAAECTALGLAAGRSAAGHVVIAENW
                     DWKPRARDTCVLLVMAPHDRPGFTTLVEAGLLAKSGMNEAGVGQVVNALVSSRDRGQA
                     GAPFHAILRRTLTSRSLDEAVDAVRRPERASSANYLLGSAKEGLADVEAAPGGPDMVT
                     VTTDDVVVHANHFLRRDRPFKDLSLLDGQDSPDRQRSVEEALEGSAALDVGDIVEALR
                     RHAGPDRGDGSVCAHGDPSLAPEADYVTIATIALDLTAGTLDLSEGNPCSAPMERFVL
                     RDLLEAARSASLPG"
     gene            complement(1058..2386)
                     /locus_tag="VIX62_06975"
     CDS             complement(1058..2386)
                     /locus_tag="VIX62_06975"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEY5860009.1"
                     /translation="MKQLHGRAWRRASMVACAALVGAATVIGATGPVAVNAKMGGPVR
                     SSYKLGQGLKLTTIRYPNAPEQVRILTITQGAGSVTDMFTPTKQYPGYRKPSAMGLLG
                     DASAMVNGDFAAPNGRPKHLSLVDGELWTSGIQDGAAIAFSGNGAHVYIGHPTLAMAA
                     KTGGTTIKVDRWNAGDPKTSQVAGFSQRGGRIEPPSGAASPTTADPRYCAARLIPTGG
                     FEWTGTGKAGIARSFTVDAQPEPCVKTPISIGTDPGAVVLTGKNGGAGADAVKLLAAG
                     EAIRLTWSFQGWPAVVDAIGAQPLLVQNGHNIAPPYHPGDSYFYNYNPRTSVGASEGC
                     SDDRSATICKTYILTVDGRQSAWSKGMQLDDLADELIRAGATWAANLDGGGGTVMWVA
                     KRGPAYCQTNVSAGGCLVNRPSERGGERVAVVSLGVKLAPDAGEPRAVRG"
     gene            2559..3929
                     /locus_tag="VIX62_06980"
     CDS             2559..3929
                     /locus_tag="VIX62_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEY5860010.1"
                     /translation="MALDPHDLPRRRHSDDLDRRVSDLPTEPSPNGKPRRRDYDGWLD
                     RWVATTSDLTRLTTTHPMMDAVIDEQVGRKIRIGDRWLTDWASCNYLGFDLDEEIIAT
                     VPEYLAKWGTHPSWSRLLGNPVLYPQIEEQLTELVGAEDTLVLPTITHIHMSVIPVLV
                     GDGAIFLDGRAHKTIYDGAMVAAGHGATVRRFRHNDFEHLEELLRASDPALPRLIAMD
                     GVNSMTGNAPELAEFSRLAREHDALLYVDDAHGFGVIGERSPGETNDYGVKGNGVFRY
                     LDVPYDNAVLVAGFSKSYSSLLAFITLPTRLKDALKVLAPPYLYSGPSPVASLATTLA
                     GLQVNRTRGDAYREDLWRMTHRLLSALHDLGIHTPNTSGFPIVEIPLSSHEDIDEVGR
                     YLFEHGQYATMAAYPLVPKNEVGFRVQVTAANTDEQVDELIEVLANLHERFQLEPNVA
                     ASRVGR"
     gene            complement(3926..4711)
                     /locus_tag="VIX62_06985"
     CDS             complement(3926..4711)
                     /locus_tag="VIX62_06985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5860011.1"
                     /translation="MDERLATSARPLAGRSALVTGGTSGIGLAVVRRFVDAGADVVAI
                     ARHARPEAEQAGARMVVCDVADATAVAGAFDQAVATVGMLDIVVLNAGIADLDDGAME
                     AMDTDGLRRMLEINTMGVFHGLAQAGPHMHDGGSVIVTATAALDWAFPDYMAYTASKA
                     PLRTMAVHAAMKLGSRGIRVNTVSPGTILTEMQPDDDPEARITPFATCLGRVGTPDDV
                     TGAYLFLAGADSAYVTGTDIRVDGGWLEGLTYASVERLLGDER"
     gene            4861..>5733
                     /locus_tag="VIX62_06990"
     CDS             4861..>5733
                     /locus_tag="VIX62_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860012.1"
                     /translation="MKKRAWLVYPVLAAAATTVYYLLGQNSLLFNLIGLTSPILIIVA
                     VKIHAPEKRWPWYLIAIGQFLFIAGDVVSYNYVLFHDVFPQLFPLDFAFNPTGDVPFP
                     GPADALYLAVYPCLITGLLLMIHARNPGRDRTSLLDSLMVAIGVATVSWVLLISPYIH
                     IADLDLKIKLTAMAYPVMDLLLAVVAIRLAVGAGRKPVAFYLMITAIGALFVTDAIYA
                     WFGLYTESGYQPGSGLLEAGWMAFYVLLGTAALHPSMVNITDKAPEWEDRLTFGRLAL
                     LTTAALAPPVVRLVQ"
BASE COUNT          871 a         2063 c         1874 g          925 t
ORIGIN      
        1 cacggttcga tcgagctgta ccgggcgatc ttccggcact acaccgggct cacgtgggat
       61 caggttcggg gccgtgccgg cgccttcgtg gagccgatcg atgccctcga cgtgcagttg
      121 ctgccggaga tggagggcat cgccgagggc gccgcggtcg acaccgaaga cgtcctggcc
      181 ctcaacgtcc gcaccgaggt gatgtacggg ctcggcgcgc cgggcgccgc cgcagccgaa
      241 tgcaccgcgc tcggactggc ggcgggccgc tcggccgcgg gccacgtcgt catcgccgag
      301 aactgggact ggaaaccgcg ggcacgggac acctgcgtcc tgctggtgat ggcgccccac
      361 gatcgcccgg gcttcaccac gctggtcgaa gccggcctgc tcgccaagtc cggcatgaac
      421 gaggccggcg tcggacaggt cgtgaacgcg ctcgtctcga gccgtgaccg gggtcaggcg
      481 ggagcgccgt tccacgcgat cctgcgacgg accctgacga gccgctcgct cgacgaggcc
      541 gtggatgcgg tacggcgccc cgagcgcgcg tcgtcggcga actacctcct gggctcggcc
      601 aaggagggcc tcgcagatgt cgaggcggct ccgggcggcc ccgacatggt caccgtgacc
      661 acggacgacg tcgtggtcca cgccaaccac ttcctccggc gggatcggcc gttcaaggat
      721 ctgtccctgc tcgacggcca ggattccccc gaccgacagc gctcggtcga ggaggcgctc
      781 gagggctcgg ccgcgctcga cgtcggtgac atcgtcgagg cgctccgccg gcacgccggg
      841 ccggatcggg gcgacggctc cgtgtgcgcg cacggcgacc cgtcgctggc gcccgaggcc
      901 gactacgtca cgatcgccac gatcgccctg gacctgacgg cgggcacgct cgacctgtcc
      961 gaaggcaacc cctgctccgc accgatggag cggttcgtcc tgcgggacct gctcgaggcc
     1021 gctcgatccg cgagcctgcc cggataaggc tgctgcctca gcctcggacc gctctcggtt
     1081 ctcctgcgtc gggggcgagc ttgacgccga gcgagaccac cgcgacacgt tcgccgccgc
     1141 gttccgacgg ccggttgacc aggcatcctc cggccgacac gttggtctgg cagtacgccg
     1201 ggccgcgctt ggccacccac atcaccgtgc caccgccgcc gtcgaggttg gccgcccagg
     1261 tcgcgccggc gcggatgagc tcatcggcca ggtcgtccag ttgcataccc ttggaccagg
     1321 cggactgtcg cccgtcgacg gtgaggatgt aggtcttgca gatcgtcgcc gaccggtcgt
     1381 ccgagcaccc ctcactcgcc ccgaccgatg tgcgggggtt gtagttgtag aagtaggagt
     1441 caccggggtg atacggcggc gcgatgttgt gcccgttctg gaccagcagt ggctgcgcgc
     1501 cgatcgcgtc gaccaccgcg ggccaccctt ggaagctcca ggtcaggcgg atcgcctccc
     1561 cggcggcgag cagcttcacg gcatcggcgc ccgccccgcc gttcttgccg gtgaggacca
     1621 ccgctcccgg atcggtaccg atcgagatgg gcgtcttgac gcacggttcg ggctgggcat
     1681 cgaccgtgaa gctccgggcg atgcccgcct tgccggtgcc ggtccactcg aaaccgccgg
     1741 tggggatcaa ccgggcggcg cagtatcgcg ggtcggccgt ggtgggcgat gcagcgcccg
     1801 acggcggttc gatgcgtccg cctcgctgcg agaagccggc cacctgggat gtcttcgggt
     1861 caccggcgtt ccagcgatcg accttgatcg tggttccacc ggtcttggcc gccatcgcca
     1921 acgtgggatg gcctatgtac acgtgggccc cgttcccgga gaacgcgatc gccgccccgt
     1981 cctggatgcc cgaggtccag agttccccgt cgaccaacga caggtgcttc ggacggccgt
     2041 tgggggccgc gaagtccccg ttgaccatgg ccgaggcatc gcccagcaat cccatcgccg
     2101 acggcttgcg gtagcccgga tactgcttgg tgggggtgaa catgtcggtg accgagcccg
     2161 cgccctgcgt gatcgtgagg atgcggacct gttcgggcgc gttggggtac ctgatcgtgg
     2221 tgagtttgag cccctgcccg agcttgtagg aggaccgaac cggtccgccc atcttcgcgt
     2281 tcaccgccac cggaccggtt gccccgatca ccgtcgccgc gccgacgagc gcggcgcacg
     2341 cgaccatcga ggcccggcgc caggccctcc cgtgcagctg cttcacgcga tgccccctcc
     2401 ccgaacgagc gtggtccgtt cgaatggtgc accgtagcga agcaggccta cgtatggaac
     2461 ccctcgttcg gcgtataggt cattcggccg agtcttcgtc ggcaaggttg ctcgcgaagg
     2521 gtaaaggagt gcgtacgtga gccgatgcag acagagccat ggcactcgac ccacacgacc
     2581 tcccccggcg tcggcacagc gacgacctgg accgccgggt ctccgacctg cccaccgaac
     2641 cctcgccgaa cggcaagcct cgtcggcggg actacgacgg ctggctggac cggtgggtgg
     2701 cgacgacctc cgacctgacc aggctcacca cgacgcaccc gatgatggac gcggtcatcg
     2761 atgaacaggt ggggcgcaag atccggatcg gcgaccgctg gctgaccgac tgggcgtcgt
     2821 gcaactacct cgggttcgac ctcgacgaag agatcatcgc gaccgtgccc gagtacctgg
     2881 cgaagtgggg cacgcacccc tcgtggtcgc gactgctggg caaccccgtc ctctaccccc
     2941 agatcgagga gcaactcacc gagctcgtgg gcgccgagga cacgctcgtg ctgccgacga
     3001 tcacccacat ccacatgtcg gtgatcccgg tgctcgtcgg cgacggcgcg atcttcctgg
     3061 acggccgtgc gcacaagacg atctacgacg gggccatggt cgcggccggc cacggcgcca
     3121 ccgttcgccg cttccgccac aacgacttcg aacacctcga ggagttgctg cgcgccagcg
     3181 atcccgcgct cccgcgcctg atcgcgatgg acggtgtcaa ctcgatgacc ggtaatgcgc
     3241 ccgagctcgc cgagttctcg cgccttgccc gggagcacga cgccttgctc tacgtcgatg
     3301 atgcccacgg gttcggcgtc atcggcgaac gttcgccggg cgagaccaac gactacgggg
     3361 tcaagggcaa cggcgtcttc cgctacctcg acgtgcccta tgacaacgcc gtgctggtgg
     3421 cagggttctc caagtcctac tcctcgctgc tggcgttcat cacgttgccc acccgactga
     3481 aggacgccct caaggtgctg gctcccccgt acctgtactc cggaccctcg ccggtggcct
     3541 cgctcgccac cacgctggcc gggttgcagg tgaaccgcac gcgtggcgac gcctaccgcg
     3601 aggacctgtg gcgcatgacc caccgcctgc tgtcggcgct ccatgacctg gggatccaca
     3661 cgccgaacac ctcggggttc ccgatcgtgg agatcccact ctcctcgcac gaagacatcg
     3721 acgaggtggg gcgctacctg ttcgagcacg ggcaatacgc cacgatggcc gcctacccgt
     3781 tggtgcccaa gaacgaggtg ggcttccgcg tgcaggtcac cgccgccaac accgacgaac
     3841 aggtcgacga gctgatcgag gtcctggcga acctgcacga gcggttccag ctggagccga
     3901 acgtcgccgc gagccgcgtc ggacgctagc gctcgtcccc gagcaaccgt tccaccgagg
     3961 cgtaggtcag cccctccagc cacccgccgt ccacccggat gtccgtgccg gtcacgtacg
     4021 cgctgtcggc gccggccagg aagaggtagg caccggtgac gtcgtccggc gtcccgaccc
     4081 ggccgaggca ggtggcgaac ggcgtgattc gagcttccgg atcgtcgtcg ggctgcatct
     4141 cggtgaggat cgtgcccggg ctcaccgtgt tcacgcggat cccgcgcgaa ccgagcttca
     4201 tggcggcgtg gacggccatg gtgcggagcg gcgccttcga cgcggtgtac gccatgtagt
     4261 ccgggaacgc ccagtcgagc gcggcggtgg cggtgacgat cacgctgccg ccgtcatgca
     4321 tgtgcggccc tgcctgcgcc agcccgtgga agacccccat cgtgttgatc tccaacatgc
     4381 gccggaggcc atcggtatcc atggcctcca tcgcaccatc gtcgaggtcg gcgatgcccg
     4441 cgttcagcac gacgatgtcg agcatcccga ccgtggccac cgcctggtcg aaggcaccgg
     4501 ccaccgccgt ggcatcggcg acgtcgcaca ccaccatccg ggcgccggcc tgctccgcct
     4561 cgggccgggc atgcctcgcg atggcgacga cgtccgcccc cgcgtcgacg aaccgacgaa
     4621 ccaccgcgag cccgatcccg gaggttcccc cggtgaccaa ggccgaccgt cccgcgaggg
     4681 gacgcgcgga tgtggcgagc cgttcatcca tgtccgaacc ctcgcacacg ccggaacccc
     4741 tcgccacgac ccgggcaagc tcggtcctga gccaacaccg caggtcaaga ggcccgcacg
     4801 ccgagcccct acccgattcc aggtattagc cgaacggtgg cactacccga taccgcattg
     4861 gtgaagaaga gggcttggct cgtctatccg gtgctcgctg cggcggctac gaccgtctac
     4921 tacctgttgg gtcagaacag cttgctgttc aacctcatcg gattgacctc gccgatcctc
     4981 atcatcgtcg cggtcaagat ccacgctccc gagaagcgat ggccctggta cctgatcgcg
     5041 atcggtcaat tcctgttcat cgccggcgat gtggtgtcgt acaactacgt cctcttccac
     5101 gacgtgttcc cgcagttgtt cccgctcgac ttcgcgttca atccgaccgg agacgtgccc
     5161 ttccccggcc cggccgacgc cttgtacctc gcggtgtatc cctgtctcat cacaggcctg
     5221 ctgctgatga tccacgcgcg caatcccggg cgggaccgca ccagcctgct ggactcgctc
     5281 atggtggcga tcggtgtagc caccgtgtcg tgggtgctgc tgatctcgcc gtacatccac
     5341 atcgccgacc tcgacctgaa gatcaagctc accgccatgg cctacccggt catggacctg
     5401 ctcctcgcgg tcgtggcgat ccgcctggcc gtcggcgccg gccgcaagcc ggtggccttc
     5461 tacctgatga tcaccgccat cggggcgctg ttcgtcaccg acgccatcta cgcctggttc
     5521 ggcctgtaca cggagtcggg ctaccaaccc ggaagcggat tgctcgaggc cggctggatg
     5581 gccttctacg tgctgctggg caccgcggcg ctgcatccgt ccatggtgaa catcaccgat
     5641 aaggcgcccg aatgggagga ccggctcacg ttcggccggc tcgccctgct caccaccgcc
     5701 gccctcgctc cccccgtggt gcgcctcgtg cag
//
LOCUS       DATDBZ010000289         7850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1102022, whole genome shotgun sequence.
ACCESSION   DATDBZ010000289 DATDBZ010000000
VERSION     DATDBZ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102022"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..494
                     /locus_tag="VIX62_06995"
     CDS             105..494
                     /locus_tag="VIX62_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860013.1"
                     /translation="MDDGAFPVRYFPRFTWLFVPLVLGPRHAAVRLTPDALHVRMGWA
                     FRAAIPRTSIQWARRAPDEPWAVGVHSNFRGRWLVNGSSLGIIELGLEPAARARTAVF
                     PVTVKVLGLGLRDPEGLLKTLGVPEHE"
     gene            complement(500..3202)
                     /gene="ppdK"
                     /locus_tag="VIX62_07000"
     CDS             complement(500..3202)
                     /gene="ppdK"
                     /locus_tag="VIX62_07000"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY5860014.1"
                     /translation="MTKYVYDFSEGNKDMKDLLGGKGANLAEMTNMGLPVPHGFTIST
                     EACLAYLDAGGGAGGYSASLADEVETHVQTLESSMGKRLGDPSDPLLVSVRSGAKFSM
                     PGMMDTVLNLGLNEESLKGLAEQSGGDMRFARDAYRRFIQMFGKIVMDVPADAFEEAL
                     DRAKDAKGEGASDTDLDADDLAALATEFLGIYRQHTGQEFPTDPREQLRLAIEAVFKS
                     WNGRRAIDYRRQNKISDSLGTAVNVQSMVFGNRGDDSGTGVAFTRDPSTGEKVPYGDY
                     LGNAQGEDVVAGIRNTLPLADLETVDSTSYTGLREVMATLEGHYRDMCDIEFTIEKGR
                     LWILQTRVGKRTAFGEWVMAYEMLDEDLIDEGEALLRVDANRLEELFKRRVDATGAEP
                     IATGLNASPGAATGAVVFTADEAQSRGQAGERVILVRRETTPDDYHGMIAAQGILTSA
                     GGTNSHAAVVARGEGIPAVCGADAIKLRVADGQFTANGTAVREGDVITIDGFSGNVFV
                     GELPLQESLLEKARQGDEAARQEPLWRSFERLMTIADERRRLRVRANADTPDQSTNAR
                     ERGAEGIGLCRTEHMFLGEERVLAVRKMIFAETAEEEQEAYDALLPLQQDDFVGIFTA
                     MSGLPVTVRLLDPPLHEFLPDQVELAVEVALAKAKGEDVTDQERVLQKVNELHEMNPM
                     LGLRGVRLGIIKPGLYAMQVRAIVEAAVRVRRDGGDPQVEIMIPLVATAPELQQMRAE
                     LEPVAREILDREGVELSHLEWGTMIELPRAAVTAGEIAESADFFSFGTNDLTQTAFGF
                     SRDDIGKFVGMYEERKLVPANPFVTIDRPGVGVLMRIATDEGRAANPAIHLGICGEHG
                     GDPASVVFCHELGLDYVSCSPFRVETARLAAGQAAVASVEGAST"
     gene            3319..3576
                     /locus_tag="VIX62_07005"
     CDS             3319..3576
                     /locus_tag="VIX62_07005"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY5860015.1"
                     /translation="MAKMVRTNVVIDERLIRKVMSLYGLETKREAVDFALHAVLEGGG
                     RASPDPWVGALELDGLWADRPKEELKSIYDDDLPEREQPAS"
     gene            3573..3971
                     /locus_tag="VIX62_07010"
     CDS             3573..3971
                     /locus_tag="VIX62_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5860016.1"
                     /translation="MILVDTSVWVDLLRGARNRTTDRLRTLLADREPIAITEPVVMEL
                     LSGCRSGHELAEVRRRLLPLDLLPVGGLETWERASAVERVCAAAGQRVRNRFDCLIAA
                     VAIREDASVLHRDRDFEIIARHTDLRIEPR"
     gene            complement(4087..5427)
                     /locus_tag="VIX62_07015"
     CDS             complement(4087..5427)
                     /locus_tag="VIX62_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860017.1"
                     /translation="MRSRRRSVAAIAVLAFVTAACTGDGIRLQIDTATPTPSVLPGND
                     GPGLRVEPVKTGEASSKGAMRALCVSPTPVSTGDGASPGPTPPQISEVEDQVEAVRGL
                     DYLDPVAADPVSADEIAEAFTSTFDDTFPKAFYDRRTVAWQTIGVIPTDVTIRDAMLA
                     FQTGQVVGFYNPVDGQLVYLGDDGDLDLIERYTLAHELTHAMDDQHFDLSRLDTIAAA
                     CRDEAFQAALGAVEGSAEFFATQVLTQFPDPDAELGDFGGAGLPAGVPPFITELTLWP
                     YSAGVAFMSALDAGGGLTAINGALSDLPPTTEQVMHPELYPQPAPEAPDIADRSAALG
                     PGWGDLDVMQVGEEWLRAMLRLALDAGDAGDAAAGWNGGTYRAWTDGHDTAVLLKTSW
                     DTPEDAAVFADAMDAWLATTGRTGSVDPVEGETVTVGFATSDESLTAVMGAPDP"
     gene            complement(5424..5630)
                     /locus_tag="VIX62_07020"
     CDS             complement(5424..5630)
                     /locus_tag="VIX62_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860018.1"
                     /translation="MSDAPAKTPRGWTTLYAVAFVVMLVAGLLLAIAARGFLDNLTLL
                     WLSAGLSLGAIVLAVLALVLPRRG"
     gene            complement(5627..6940)
                     /locus_tag="VIX62_07025"
     CDS             complement(5627..6940)
                     /locus_tag="VIX62_07025"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY5860019.1"
                     /translation="MEAVTSLAKRRGIAFQSAEIYGGLRSSWDYGPLGVELRRNIRNA
                     WWRQTVQLRDDVVGVEAAIITSPKVWEASGHLETFHDPLIECTNCHQRFREDHLEANA
                     DGVIACPNCGGAEFTEPKQFNLMLKTFLGPIEDDDAVAYLRPETAQGMFVNFALIQAT
                     ARKKLPFGIAQIGKSFRNEITPGNFIFRTREFEQMEMEFFVEPGTDDEWFDTWVQQRL
                     QWYLDLGVSKEKLRLRPHETDELAHYAKAATDVEYEFPFGWSELEGIANRADYDLRAH
                     QEASGEDLTFFDQERETRYLPYVIEPAVGVDRAMLTFLLDAYRVEEVPTAKGDTDKRT
                     VLGLHRDLAPIKVAVLPLSRNEKLVPEARRVFDLVKPEWMSQYDDAGAIGRRYRRQDE
                     VGTPFCVTVDFDSLDDRQVTVRERDTMHQDRIPIVNLLDYLQERL"
     gene            complement(7232..>7850)
                     /gene="uppS"
                     /locus_tag="VIX62_07030"
     CDS             complement(7232..>7850)
                     /gene="uppS"
                     /locus_tag="VIX62_07030"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY5860020.1"
                     /translation="GEEALWDTVKGANEVGVRWLTVYAFSTENWKRPAPEVLYLMNFN
                     RDLLRRRRDELNDMNVRIRWTGRRDWRVPRSVLKGMRISEELTRDNTGMTLTIAFNYG
                     GRAEIVDAVKDLARDVREGTLKPEKVTEREIAQRLYHPDMPEPDLLIRTSGEERVSNF
                     LLWEVAYSELWFTPVFWPDFTRETLFEAIRDFQKRTRRFGAIAED"
BASE COUNT         1176 a         2702 c         2643 g         1329 t
ORIGIN      
        1 ggggaggtgg gaggacggca gaggctacgt cagggcgggg cgccccgccg tgacggaggt
       61 cacggttcga gcaccacgag acgctctgga gtagcgtgcc tcccgtggac gacggtgcgt
      121 tcccggttcg ctacttcccc cggttcacct ggctgttcgt gccgctcgtc ctcggtccga
      181 ggcacgcggc ggtccgactc acgcccgatg ccctccacgt acggatggga tgggcgttcc
      241 gagccgcgat cccgcggacg tcgatccaat gggcgcggcg ggcccccgac gaaccctggg
      301 ccgtcggtgt ccactcgaac ttccggggac gctggctggt gaacggttcg tcgctgggca
      361 tcatcgaact cggcctggag ccggcggccc gcgcccgcac cgccgtcttc ccggtcaccg
      421 tgaaggtgct gggtctcggg ctccgagatc ccgagggcct gctgaagacc ctgggtgtgc
      481 ccgagcacga gtgagcgcgc tacgtcgaag caccctccac ggaggccacg gccgcctgac
      541 ccgccgcgag gcgcgcggtc tccacgcgga agggcgagca actgacgtag tccaacccca
      601 gttcgtggca gaagacgacg ctcgcggggt caccgccgtg ctcaccgcag atcccgaggt
      661 ggatggcggg gttcgcggct cggccttcgt cggtggcgat ccgcatcagc acccccaccc
      721 ccgggcggtc gatcgtgacg aacgggttcg cgggcacgag cttgcgctct tcgtacatgc
      781 cgacgaactt gccgatgtcg tcgcggctga atccgaacgc ggtctgggtg aggtcgttgg
      841 tgccgaacga gaagaagtcg gcgctctcgg cgatctcgcc ggcggtgacc gcggcgcgcg
      901 gcagctcgat catcgtgccc cactccaggt ggctcagctc gacgccttct cgatcgagga
      961 tctcgcgggc caccggttcg agctcggcgc gcatctgctg gagctcgggc gcggtggcga
     1021 ccagcgggat catgatctcc acctgtgggt cgccgccgtc ccggcgcacc cggacggccg
     1081 cttccacgat cgcgcgcacc tgcatcgcat acagcccggg cttgatgatg cccaggcgca
     1141 cgccgcggag gccgagcatc ggattcatct cgtggagttc gttcaccttc tgcagcacgc
     1201 gttcctggtc ggtgacgtcc tcgcccttgg ccttggcgag cgccacctcg acggcgagct
     1261 ccacctggtc gggcaggaac tcgtgcagcg gcgggtcgag caaccgcacc gtgaccggca
     1321 acccgctcat cgcggtgaag atgcccacga agtcgtcctg ctgcaagggc aagagagcgt
     1381 cgtaggcctc ttgttcctct tccgccgtct cggcgaagat catcttgcgg accgccagga
     1441 cccgctcctc cccgaggaac atgtgctcgg tgcggcagag gccgatgccc tcggcgcccc
     1501 gctcgcgcgc gttcgtcgac tggtcggggg tgtcggcgtt ggcgcgaacg cgcaagcgcc
     1561 ggcgctcgtc agcgatcgtc atcaagcgct cgaacgaacg ccacagcggt tcctggcgcg
     1621 cggcctcgtc cccttgccgc gccttctcga gcaacgattc ctggaggggc agttcgccga
     1681 cgaagacgtt gccgctgaac ccgtcgatcg tgatgacgtc accctcgcgc acggcggtgc
     1741 cgttcgcggt gaactgcccg tcggccaccc ggagcttgat cgcgtcggcg ccgcacaccg
     1801 ccgggatgcc ctccccgcgc gccaccacgg ccgcgtgtga gttcgtccca ccggcgctcg
     1861 tcaggatgcc ctgggccgcg atcatgccgt gatagtcgtc cggcgtggtc tctcgacgga
     1921 ccaggatgac ccgctcgccc gcctggcctc gcgactgcgc ctcgtcggcg gtgaacacca
     1981 ccgcgccggt ggccgcaccg ggcgatgcgt tgaggccggt cgcgatcggc tcggcgccgg
     2041 tcgcatcgac ccgccgcttg aacagttcct ccagtcggtt ggcgtcgacg cgcagcagcg
     2101 cttcgccctc gtcgatcagg tcctcgtcga gcatctcgta ggccatgacc cattcgccga
     2161 aggcggtgcg cttgcccacc cgcgtctgca ggatccacag ccggcccttc tcgatcgtga
     2221 actcgatgtc gcacatgtcg cggtagtgac cctcgagcgt ggccatcact tctcggagtc
     2281 ccgtgtacga cgttgaatca accgtctcca ggtcggcgag cggcagggtg ttgcggatgc
     2341 ccgccacgac gtcctcgccc tgcgcgttgc ccaggtagtc gccgtagggc accttctcgc
     2401 ccgtgctggg gtcacgcgtg aacgccacac ccgtgccgga atcgtcgccg cggttgccga
     2461 acaccatcga ctgcacgttc accgcggtgc cgagcgagtc gctgatcttg ttctggcgcc
     2521 ggtaatcgat cgcgcgccgg ccgttccagc tcttgaagac cgcttcgatc gccaggcgca
     2581 gctgctcccg cgggtccgtc gggaactcct ggccggtgtg ctggcggtag atgcccagga
     2641 actccgtcgc cagcgccgcc aggtcgtcgg cgtcgaggtc ggtgtccgag gcgccctcgc
     2701 ccttggcgtc cttcgcgcga tcgagcgctt cctcgaaggc gtcggccggc acgtccatca
     2761 cgatcttgcc gaacatctgg atgaaccggc ggtaggcatc gcgcgcgaac cgcatgtcac
     2821 cgccggactg ctcggccagc cctttcagcg actcctcgtt gaggcccagg ttcaggacgg
     2881 tgtccatcat gccgggcatc gagaacttgg cgccggaccg gacgctgacc aacaacgggt
     2941 cgctgggatc gccgagccgc ttgcccatgg aggactcgag cgtctgcacg tgggtctcga
     3001 cctcgtccgc gaggcttgct gagtacccgc ccgccccacc accggcgtcg aggtaggcca
     3061 ggcacgcctc ggtgctgatc gtgaacccgt ggggcaccgg caggcccatg ttcgtcatct
     3121 cggcgaggtt ggcccccttg ccgcccagca ggtccttcat gtccttgttg ccttcagaga
     3181 agtcgtagac gtacttggtc actgcatgcc tccggcaggg tcgagcggtt cggagtcgtc
     3241 agtctacggc gagtgtgcgc ccggatggcg gcagggaggt ggtacacatc actgtgtaga
     3301 ggccggtaga ctggcaggat ggcgaagatg gttcgcacca acgtggtcat cgatgaacgg
     3361 ctgatccgca aggtcatgag cctgtacggg ctcgagacga aacgggaagc cgtcgatttc
     3421 gccctgcatg ccgtgctcga agggggcggt cgcgcgagtc ccgacccatg ggtaggcgcc
     3481 ctcgaactcg acggcctctg ggcggatcga ccgaaggagg agctgaagtc gatctacgac
     3541 gatgatctcc ccgagcgtga gcagccggct tcgtgatcct cgtcgacacc tcggtgtggg
     3601 tcgacctgct ccgcggcgca aggaaccgaa ccaccgatcg gctccgaacg ctcctcgccg
     3661 accgggagcc catcgccatc accgaacctg tcgtgatgga gctcctgtcg ggatgccgtt
     3721 cgggtcacga actcgccgag gtccggcgcc gtctcctgcc actggatctc ctgcccgtcg
     3781 gtggcctcga gacgtgggaa cgagcgtccg cggtcgagcg agtgtgtgcc gccgccggcc
     3841 agcgggtccg gaaccggttc gattgcctca tcgcggctgt agcgatccgc gaggatgcct
     3901 ccgtccttca tcgcgatcgc gacttcgaga tcatcgccag gcacaccgac ctccgcatcg
     3961 aaccgcgttg acccccgagg tcgcctgacc gtcaggcgcc actcacgtga cgaccaaggc
     4021 gatcatggca gcgacggggt tgatccggag cacggagccc atcctggccc aggcgctagc
     4081 tcgtcgctag gggtccggtg cccccatgac ggccgtcagg gactcgtcgc tggtcgcgaa
     4141 cccgaccgtc accgtctcgc cctcgaccgg atccaccgaa ccggtccggc cggtcgtggc
     4201 gagccaggca tccatcgcat cggcgaacac ggcagcgtcc tcgggcgtgt cccacgaggt
     4261 tttcagcaac accgcggtgt cgtgtccgtc ggtccaagcc cggtaggtgc cgccgttcca
     4321 tccggcggcc gcgtcgccgg catcgccggc atccagcgcg agtcggagca tggcgcggag
     4381 ccactcctcc cccacctgca tcacgtccag atctccccac cccggtccga gggccgccga
     4441 acgatcggcg atgtcgggcg cctccggtgc cggttgcggg tagagctccg gatgcatcac
     4501 ctgttcggtg gtgggcggca gatccgagag cgcgccgttg atcgctgtga gcccacctcc
     4561 cgcgtccaac gcgctcatga acgccacacc cgccgagtag ggccacaagg tgagctcggt
     4621 gatgaagggc ggtaccccgg cgggaaggcc cgctccgccg aagtcgccga gctccgcatc
     4681 ggggtcaggg aactgcgtga gcacctgcgt ggcgaagaac tccgcactcc cctcgacggc
     4741 gcccaaggct gcctggaacg cttcgtcgcg gcacgccgcc gcgatcgtgt cgagccgcga
     4801 cagatcgaaa tgctggtcgt ccatcgcatg cgtcagctcg tgcgccagcg tgtagcgctc
     4861 gatgagatcg aggtcgccgt cgtcgcccag gtagacgagc tggccgtcca cggggttgta
     4921 gaagcccacc acctgaccgg tctggaacgc cagcatcgca tcccggatcg tgacgtcggt
     4981 cgggatcaca ccgatggtct gccaggcgac cgtccgtcgg tcgtagaacg ccttcgggaa
     5041 ggtgtcgtcg aacgtcgacg tgaaggcctc agcgatctcg tcagcgctca ccgggtcggc
     5101 cgccaccgga tccaggtaat cgaggccgcg caccgcttcc acctggtcct cgacctcgga
     5161 gatctggggt ggcgtagggc cgggcgaagc accgtcaccg gtcgacaccg gcgtgggtga
     5221 gacgcatagg gcgcgcatcg cgcccttcga gctggcttcc ccggtcttca ccggctcgac
     5281 ccgcagccct ggaccgtcgt tgccgggcag gaccgatggc gtgggggtgg ccgtgtcgat
     5341 ctgcaaacgg atgccgtcgc cggtgcacgc cgcggtgacg aacgccagca ccgcgatcgc
     5401 cgccaccgag cggcggcgcg acctcatccc cgcctcggca gcaccagcgc cagcaccgcg
     5461 aggacgatcg ctcccaggga gagcccggcc gacaaccaca ggagcgtgag gttgtcgagg
     5521 aacccgcgag ccgcgatcgc cagcagcagg ccggcgacca acatcaccac gaacgccacg
     5581 gcgtacaggg tggtccagcc ccgtggcgtc ttggcgggcg cgtcgctcac aggcgctcct
     5641 gcaggtagtc aagcaggttg acgatcggga tccgatcctg gtgcatcgtg tcgcgctcgc
     5701 gcaccgtcac ctgccgatcg tccagcgaat cgaagtcgac ggtgacgcag aacggtgttc
     5761 ccacctcgtc ctggcgacgg tagcggcgac cgatcgcgcc ggcgtcgtcg tactggctca
     5821 tccactcggg cttgaccaga tcgaagaccc tacgcgcctc gggcacgagc ttctcgttgc
     5881 gcgacagcgg cagcaccgcg accttgatgg gcgcgagatc gcggtgcaga cccagcacgg
     5941 tgcgcttgtc tgtgtcgcct ttggccgtcg gtacctcctc cacgcggtag gcgtcgagca
     6001 ggaacgtgag catcgcgcgg tccacgccca ccgcgggttc gatcacgtag ggcaggtacc
     6061 gcgtctctcg ctcctggtcg aagaaggtga ggtcttcacc gctcgcctcc tggtgggcac
     6121 gcaggtcgta gtcggcgcgg ttcgcgatcc cttcgagttc gctccagccg aacgggaact
     6181 cgtactcgac gtccgtggcg gccttcgcgt agtgggccag ctcgtcggtc tcgtgcgggc
     6241 gcaggcgcag cttctccttc gagacgccga ggtccaggta ccactgcagg cgttgctgca
     6301 cccaggtgtc gaaccactcg tcgtcggtgc cgggttcgac gaagaactcc atctccatct
     6361 gttcgaactc gcgcgtgcgg aagatgaagt tgcccggcgt gatctcgttg cggaacgact
     6421 tgccgatctg cgcgatcccg aacggcagct tcttgcgcgc cgtggcctgg atcagggcga
     6481 agttcacgaa catgccctgc gcggtctcgg gacggaggta ggcgaccgcg tcgtcgtcct
     6541 cgatcgggcc gaggaacgtc ttcagcatca ggttgaactg cttgggctcg gtgaactcgg
     6601 cgcccccgca gttcgggcac gcgatcacgc cgtcggcgtt cgcctcgagg tggtcctcac
     6661 ggaaccgctg atggcagttc gtgcactcga tcagcggatc gtggaacgtc tcgaggtggc
     6721 cgctcgcttc ccacaccttg ggcgaggtga tgatggcggc ttcgacgccg acgacgtcgt
     6781 cgcgcagttg caccgtctga cgccaccatg cattgcggat gttccgacgg agctcgaccc
     6841 cgagcgggcc gtagtcccag gacgagcgca gccccccgta gatctcagcg gactggaacg
     6901 cgatccctcg tcgtttcgcg agggaggtga cggcctccat gaggtcggtc atgtgcgagg
     6961 actcctctcc cggccgctgg cagcgaccgc tggcgtagac ccatcgtacc ggcgggcatg
     7021 cgcggcggga gcggtcgcca tcgaagcgtc acgacccgcg tgggcgggag cgatgggaaa
     7081 ccggatctga gatgaggagc gtcgctcgtg ctgtggagtc cttcaggatc tcgtttccgg
     7141 tatgagtggg gagcatccga aggttcgagt cgatcgcata ccggatgcgg tatcgcggat
     7201 gcagcccaga agccggcccc gccggaacgc cctagtcctc ggcgatcgcg ccgaacctgc
     7261 gggttcgctt ctggaagtcg cggatcgcct cgaacagggt ctcgcgggtg aagtcgggcc
     7321 agaacaccgg cgtgaaccac agctcggagt aggccacctc ccacaggagg aagttcgaga
     7381 cgcgttcttc cccgctggtg cggatcagca ggtcgggttc gggcatgtcg gggtggtaga
     7441 gacgctgggc gatctcgcgt tcggtgacct tctcgggctt cagcgtgccc tcgcggacgt
     7501 cgcgagccag gtccttcacg gcgtcgacga tctcggcgcg cccgccgtag ttgaaggcga
     7561 tcgtcagcgt catgccggtg ttgtcgcgcg tcagctcttc ggagatccgc atccccttca
     7621 gcaccgacct gggcacccgc cagtcgcggc ggccggtcca gcggatccgc acgttcatgt
     7681 cgttcagctc gtcgcggcgg cggcggagca ggtcgcggtt gaagttcatg aggtagagca
     7741 cctcgggggc ggggcgcttc cagttctcgg tgctgaacgc gtagaccgtg agccagcgca
     7801 cgcccacctc gttggcgccc ttcaccgtgt cccagagcgc ttcctcgccg
//
LOCUS       DATDBZ010000290         5751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_73405,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000290 DATDBZ010000000
VERSION     DATDBZ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73405"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="VIX62_07035"
     CDS             complement(<1..1669)
                     /locus_tag="VIX62_07035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5860021.1"
                     /translation="MEREEGQTADAVASRLATLIEPGSLLEATPECLVVAHADGRILF
                     ANHRVEQLTGFDRTDLVGQSVELLIATDILELVPGTRLETICRTNDLGEIPVEIHLGT
                     IEGPERLLVVSLRDVSDLKAGREAQFEAEAKYRALVEQIPAVVYLDPVDENSDSIYVS
                     PQVVTLLGIDQDEWLTDPYCWRRHVHPEDLDRAWEEYEDAYNNHTTLIHEYRMLHEDG
                     SIRWVLEQAYPINDEHGNPWLIQGVIFDISARKAAEEQVAFLAYHDKLTGLPNRALFE
                     EMLESAITRARRHDLGVGVLFLDLDNFKLVNDSLGHHAGDVLLAQLGERLKGCTRETD
                     MVARQGGDEFLLLLSDLERGAGPIPGTEAALIVAESVAERVHEALQEPFNLNGTEFIA
                     SGSVGISLFPQDALDAATLLKNADAAMYQSKKQDPGGYVIYATSGEDPMKKLSMTTRL
                     RRAVEQENWILHYQPVVNLRDGSVEGVEALVRWQDPNGGIVPPGEFIPLAEELGLIEA
                     IGDWVIDAMAAQQRAWASDGLDLTVGFNLSPRQLWSAHLAEKVLAKLR"
     gene            1788..2228
                     /locus_tag="VIX62_07040"
     CDS             1788..2228
                     /locus_tag="VIX62_07040"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5860022.1"
                     /translation="MAHHVEREITTICRRERAFDFVADFSTTQEWDPGIQAATRLGDR
                     PIGVGSRFELVSRFGSTEQTIVYEIAAYDRPTSVTFVGDGKTFRGTDVISFSEDDAGT
                     RVTYVADLGLKGLTALAMPFLRGKLDAMSDDAVDGLRRALDARA"
     gene            complement(2235..3467)
                     /locus_tag="VIX62_07045"
     CDS             complement(2235..3467)
                     /locus_tag="VIX62_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5860023.1"
                     /translation="MRILVVGSGGVGAAFAPIAARREFYEHIVFADLDPSRSKRVVDR
                     YGAGGKFSATTVDASDAANVAECIRANDITHVLNAADPRFVMPIFDGVFEAGAHYMDM
                     AMSLSTKHPDAPYERVGKKLGDDQFAVADRWVERGLLALCGLGIEPGAADVFAKYAAT
                     HLFGKVDEVGVRDGANLEIAGYEFAPTFSIWTTIEECLNPPVIWEKSREGVDGGWYTT
                     APFSEPEMFTFPEGIGDVECVNVEHEEVLLVPRWIDCDRVTFKYGLGDEFINVLEVLH
                     KTGLDRVETVKVRGVEVSPRDVVASTLPDPAFLGEEMTGKTCAGTWVKGTGTDGNPRE
                     VYLYHVVDTEETWAKDGAQAVTWQTAINPVIGLELLASGTWSGTGVLGPEAFEPMAFL
                     DLLIDYGAPWGMTEMTPS"
     gene            3535..3663
                     /locus_tag="VIX62_07050"
     CDS             3535..3663
                     /locus_tag="VIX62_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860024.1"
                     /translation="MGPIKRLLAKLRGRGQDEDVRRQLERENEGLWKKRIPPTPRR"
     gene            3752..4519
                     /locus_tag="VIX62_07055"
     CDS             3752..4519
                     /locus_tag="VIX62_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860025.1"
                     /translation="MMAFPGGERFRRLSGAALAALLAVAIVVVVAGAGARNVPATTAF
                     GSVGASEPPAQHPAVPLPAWWPKRATLLTDSVGLGAVAALREAMPDWHLKVLGHPALM
                     VDEAADDLVEHRTHVDKVVVVALGYNSLWERGREDFDYWSGLFDRNARRMVRTLHAAG
                     ARKIVWVTLRDAPRSAIAPEDLEQFDSFAWYFPWVNQRLQALDRERTDVALADWARLG
                     DRRGITYDAIHLDPDGAALYARMVRHAILTEPYRAQA"
     gene            4516..5463
                     /locus_tag="VIX62_07060"
     CDS             4516..5463
                     /locus_tag="VIX62_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860026.1"
                     /translation="MKATRAIAVGVVVAAACVVPAPASMAAFPGDDGLIAFSYESPVN
                     GEHLTQNDIYTMEPAGTNLQQLTDTPHRQEFAPAWNADGTKIAFWRTKAPIGPGTIWV
                     MDADGSNPVRLTEDIDARDPVWSPNGRRIAFSSYVGSSSNNIYTMRASDGGGRVRITP
                     WPSREFEPAWSPDGESIAFTRGFERGDVGDIWSIELATGQATQLTATPAYDHQVAWFP
                     DSAAVVFERAKFVTAKIMTVDADGNGKSALTSGFFDANPAPSPTGGTIVFASDRIGGF
                     LPDLWMMQADGASPQVILDLPFASTTPDWQPVPVPGRQA"
     gene            complement(5467..>5751)
                     /locus_tag="VIX62_07065"
     CDS             complement(5467..>5751)
                     /locus_tag="VIX62_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860027.1"
                     /translation="TIMATGVPSGGTVRLIRGPADLAGPNAPDPGTTVTSYPASQFAG
                     GSLTVPVATATSAYVRVEVVDSTGRLKASSNPVWLLRAAPGGIPDARTVG"
BASE COUNT          891 a         2030 c         1895 g          935 t
ORIGIN      
        1 cacgcagctt ggccagcacc ttctcggcca agtgtgccga ccacagctgc ctcggtgaca
       61 ggttgaaccc gacggtgagg tcgaggccgt ccgaggccca ggcccgctgt tgcgccgcca
      121 tcgcgtcgat cacccagtcg ccgatcgcct cgatcagtcc gagctcctcg gcgagcggga
      181 tgaactcccc cggcggcacg atgccgccgt tcgggtcctg ccaccgaaca agcgcctcca
      241 ccccttccac cgagccgtcg cgaaggttca cgaccggctg gtagtgcagg atccagttct
      301 cctgctccac cgcacggcga aggcgcgtgg tcatcgagag cttcttcatc ggatcttcgc
      361 cgctcgtggc atagatcacg tagccgcccg gatcctgctt cttggactgg tacatcgcgg
      421 cgtcggcgtt cttcagcaag gttgcggcgt cgagcgcgtc ctgcgggaac agggagatcc
      481 ccaccgagcc gctggcgatg aactccgtgc cgttcaggtt gaacggttcc tgcagtgctt
      541 cgtgcacgcg ctcggccacg gattcggcca cgatcagcgc cgcttcggtt ccggggatcg
      601 ggccggcgcc acgctccagg tccgagagca ggagcaggaa ttcgtcgcct ccctggcgag
      661 ccaccatgtc ggtctcgcgc gtgcagccct tcaagcgttc gccgagctgg gccagcagga
      721 cgtcgccggc gtggtggccc agcgagtcgt tcacgagctt gaagttgtcc aggtccagga
      781 acaggacgcc cacaccgaga tcgtgccggc gggctcgcgt gatggcgctc tcgagcatct
      841 cctcgaacag cgcgcggttc ggcaggccgg tcagcttgtc gtggtaggcc aggaacgcga
      901 cctgttcctc ggcggccttc cgggcgctga tgtcgaagat cacgccttgg atgagccacg
      961 ggttgccgtg ctcgtcgttg atcgggtagg cctgttccaa cacccatcgg atcgaaccgt
     1021 cctcgtgcag catgcggtac tcgtggatga gggtggtgtg gttgttgtag gcgtcctcgt
     1081 actcctccca ggctcggtcg aggtcctcgg gatggacgtg cctgcgccag caatacggat
     1141 cggtcagcca ctcgtcctgg tcgatcccga gcagcgtcac cacctgcgga ctcacgtaga
     1201 tcgagtccga gttctcgtcg actgggtcca ggtagacgac cgccgggatc tgctcgacca
     1261 gtgcccggta cttcgcctcg gcctcgaact gcgcctcgcg tccggccttg agatccgaca
     1321 cgtcgcggag ggagaccacg agcaaccgct ccggaccctc gatcgtcccc agatggatct
     1381 ccacggggat ctcaccgagg tcgttcgtgc ggcagatcgt ctccagccgc gtcccgggga
     1441 ccagctcgag gatgtcggtg gcgatcagca actccaccga ctgacccacc agatcggtgc
     1501 ggtcgaatcc ggtgagctgt tccacacggt ggttggcgaa caggatgcga ccgtccgcat
     1561 gggccaccac caggcattcc ggggtggcct ccaggaggga accgggctcg atcagggtgg
     1621 cgagccggga cgcgacggcg tccgccgtct gtccttcctc gcgttccatc accatctgcg
     1681 ccctccggcg ttcggccttg gtgaggcatc ggacgagaac ccatgcaacc tgagcgttcc
     1741 ggccttgcac gcagtggttc ccggcatccg cccgtaggct cgaacgcatg gcccaccacg
     1801 tcgaacgcga gatcaccacg atctgccgcc gtgagcgggc gttcgacttc gtggcggact
     1861 tctccaccac ccaggaatgg gaccccggga tccaggccgc cacacggctc ggagaccgtc
     1921 cgatcggggt gggatcacgc ttcgagctgg tgtcgcggtt cggctcgacc gagcagacga
     1981 tcgtgtacga gatcgccgcc tacgaccgtc cgaccagcgt gaccttcgtg ggtgacggga
     2041 agaccttccg agggaccgat gtcatctcgt tctccgagga cgacgccgga acgcgcgtga
     2101 cctacgtcgc cgacctcggg ctgaaagggc tcacggcgct ggcgatgccg ttcctcaggg
     2161 gcaagctcga cgcgatgagc gacgacgccg tcgacggtct ccgacgagcc ctggacgcgc
     2221 gcgcctagcg agcgctagct cggcgtcatc tccgtcatgc cccacggcgc gccgtagtcg
     2281 atcaacaggt ccaggaacgc catcggctcg aacgcttcag gtcccagcac gcccgtgccg
     2341 ctccaggtgc cgctggccag cagttcgagg ccgatcacgg ggttgatcgc ggtctgccac
     2401 gtgacggcct gcgcaccgtc cttggcccag gtctcttcgg tgtccaccac gtggtacaag
     2461 tagacctctc gagggttccc gtcggtgccg gtgcctttca cccaggtgcc ggcgcacgtc
     2521 ttgccggtca tctcctcgcc caggaacgcg gggtcgggca gcgtcgaggc caccacgtcg
     2581 cgggggctca cctccacccc acggaccttc acggtctcca cccggtccag gccggtcttg
     2641 tgcagcacct cgagcacgtt gatgaactca tcgcccagcc cgtacttgaa ggtcacccgg
     2701 tcgcagtcga tccaccgcgg gaccagcagg acctcttcgt gctcgacgtt gacgcactcg
     2761 acgtccccga tgccctcggg gaacgtgaac atctcgggtt cgctgaacgg tgccgtggtg
     2821 taccaaccgc catcgacgcc ctcgcgactt ttctcccaga tcaccggagg gttgaggcat
     2881 tcctcgatcg tggtccagat gctgaacgtc ggcgcgaact cgtagccggc gatctccagg
     2941 ttggcgccgt cgcgcacacc gacctcgtcc accttgccga acaggtgggt cgcggcgtac
     3001 ttcgcgaaca cgtcggcggc gccgggttcg atgcccaggc cgcagagggc gagcaagccc
     3061 cgttccaccc atcggtccgc caccgcgaac tggtcgtcac ccagcttctt gcccacccgc
     3121 tcgtacgggg cgtcggggtg cttggtcgac agcgacatcg ccatgtccat gtagtgggcg
     3181 ccggcctcga acaccccgtc gaagatcggc atcacgaacc gtgggtcggc ggcgttcaac
     3241 acgtgggtga tgtcgttggc gcggatgcac tcggcgacgt tcgccgcatc ggaggcatcg
     3301 accgtcgtcg cggagaactt cccacccgct ccgtagcgat ccaccacgcg cttggaccgc
     3361 gacgggtcca gatcggcgaa cacgatgtgc tcgtagaact cacgtctggc ggcgatcggt
     3421 gcgaacgctg cccccactcc cccggagccc accaccagga tcctcatggc gcgggagtct
     3481 acagaagcca cgcgcgtcac ccgttggacc cttcaggggg cgtatcctgc gcgcatgggc
     3541 ccgatcaagc gcctcctcgc caagctccgc ggccgcggcc aggacgaaga cgtccgccgc
     3601 cagctcgagc gcgagaacga agggctctgg aagaagcgga tcccgccaac gccccgcagg
     3661 tgactcggag cgcatcgatc cgaccggcgc agtagcatga ccctccggcc gaggtcggag
     3721 cggcgttccc ggttcgaaag gttccttccc gatgatggcg ttcccaggag gcgagcggtt
     3781 ccgacgtctg tcgggcgcag ccctggcggc gctcctggcc gtcgccatcg tcgtcgtggt
     3841 ggcgggagcc ggcgctcgca acgtgccggc cacgacggcg ttcggctcgg tcggcgcatc
     3901 cgaacccccg gcccagcacc cggccgtccc actcccggcg tggtggccga aacgcgcgac
     3961 cctgctcacc gactcggtgg ggctcggtgc cgtcgccgcg ctccgcgagg ccatgcccga
     4021 ctggcacctg aaggtgctgg ggcatccggc cctgatggtg gacgaggcag ccgatgatct
     4081 ggtcgagcac cggacccacg tggacaaggt cgtcgtcgtg gccctcggct acaactccct
     4141 gtgggaacgc ggccgcgagg acttcgacta ctggtcgggg ctgttcgacc gcaacgcccg
     4201 ccggatggtt cggaccctgc acgcggccgg cgcgcgcaag atcgtctggg tgacgctacg
     4261 cgacgcgccc agatcggcga tcgcacccga ggacctggag cagttcgatt cgttcgcctg
     4321 gtacttcccc tgggtcaatc agcgtttgca ggcgctagac cgggagcgca ccgacgtggc
     4381 gctcgcggat tgggcccggt tgggcgaccg gcgaggcatc acctacgacg ccatccacct
     4441 cgacccggac ggtgcggcgc tctacgctcg catggtgcga cacgcgatcc tcaccgagcc
     4501 ataccgagca caggcgtgaa ggccacccgc gcgatcgcgg tgggcgtggt ggtcgccgcg
     4561 gcctgcgtcg ttccggcgcc cgcgtccatg gccgcgttcc cgggcgatga cgggctgatc
     4621 gccttctcct acgagtcgcc cgtcaacggc gagcacctca cgcagaacga catctacacg
     4681 atggagcccg cgggcacgaa cctgcaacag ctcaccgaca ccccgcaccg tcaggagttc
     4741 gcgcccgcgt ggaacgccga cggcacgaag atcgcgttct ggcggacgaa ggcgccgatc
     4801 ggcccaggca cgatctgggt gatggacgcc gacggcagca accccgtccg gttgaccgag
     4861 gacatcgacg cccgcgaccc cgtgtggtcg ccgaacggcc ggcggatcgc gttcagttcg
     4921 tacgtgggat cgagctccaa caacatctac acgatgcgcg cctccgacgg cggagggcgc
     4981 gtgcgcatca cgccgtggcc cagccgcgag ttcgagcccg cgtggtcgcc ggacggggaa
     5041 tcgatcgcct tcacccgcgg gttcgagcgg ggcgatgtcg gtgacatctg gtcgatcgaa
     5101 ctggcgacgg gccaggcgac ccagctgacc gcgacgccgg cctacgacca ccaggtggcg
     5161 tggttcccgg attcggcagc ggtggtgttc gagcgggcga agttcgtcac ggccaagatc
     5221 atgacggtcg acgcggacgg caacggtaag tcggcgctga cctcgggctt cttcgacgcc
     5281 aacccggccc catcgccgac gggcggcacg atcgtattcg cgagcgaccg catcggtggc
     5341 ttcctgccgg acctgtggat gatgcaggcc gacggtgcgt cgccccaggt gatcctcgac
     5401 ctgccgttcg cctcgaccac gcccgactgg cagcccgtgc cggtgccggg acggcaggcc
     5461 tgagcctcac cccaccgtgc gagcgtccgg gatccccccg ggcgcggccc tcagcagcca
     5521 caccgggttg gagctggcct tgagcctgcc cgtcgaatcc acgacctcga cccggacgta
     5581 cgccgacgtc gcggtggcca ccggaaccgt gaggctccca ccggcgaact gggaggccgg
     5641 gtagctcgtg accgtggttc ccgggtcggg ggcgttcggg cccgccaggt cggccggtcc
     5701 tcggatcagc cgcaccgttc cgcccgacgg cacgcccgtc gccatgatcg t
//
LOCUS       DATDBZ010000291         4962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1938425, whole genome shotgun sequence.
ACCESSION   DATDBZ010000291 DATDBZ010000000
VERSION     DATDBZ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938425"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2024)
                     /locus_tag="VIX62_07070"
     CDS             complement(<1..2024)
                     /locus_tag="VIX62_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5860028.1"
                     /translation="MTGRRYVVIGAGVVGAAVADELTARGATDVTVLDKGPLYATGGS
                     SSHAPGLVSRTSPSKMMQALADYTVDKFTVLDLDGAPAMLPVGTLEVARNEPRLRELQ
                     RRWNATTAWGFRGRMVEPAEIAQLWPIIDTAGLVGGYVTEGEGLAVALRAVEAQARRA
                     IARGARFEGDTEVTGIEHRDGRVTGVRVGGEIIPADVVVCCAGVWGPTVASMVGLTLP
                     MLALEHQYVVTTPVPALAANSGNEATMPIVRHHDAGLYYRDHGDRVGIGSFNHRRLPV
                     AATGLDTHERREDGIVYAFTPEDFAEPWELTCQLMPALRETMLERSFNGVFAFTPDGY
                     PLIGEHPDLRGFWVGESVWVTHSAGVGRVLAETLIDGAPSIDASPADLSRFERPELDP
                     AVFEARCDDSYRDVYAVHHPAEGHTSARDLRFSPFEPRQRELGAAFFDTAAWERPRWY
                     EANHRLADAAGVPQRDEWSSRYWSPIAIAEHLAVRERAGLFDMTALTRIEVSGPGAEA
                     FLGWMVAGRVDRPVGTVTYSLLLDVRGGVRSDITVARVAEDRFVIGANGPRDLAWLRR
                     HLPPDGSVTVTDLTGAVCCLGLWGPAARDILAPVADGDLAFGYLRVGELRVKGIPVTA
                     LRVSYAGELGWELTAAFDDGLAVWDALMASGERYGVIAAGVAALSSLRIE"
     gene            complement(2028..2660)
                     /locus_tag="VIX62_07075"
     CDS             complement(2028..2660)
                     /locus_tag="VIX62_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017538583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5860029.1"
                     /translation="MPPRVDLVSTIASRDYPGEGIVEVQNNVWIVGGDDEVVVIDASH
                     DASPIVDAIGGRRVLAIACTHGHWDHVDAAFDVQAAVDAPILMHPADRPVWERTHAER
                     QPDGPLDEGALLVAGGVELRVIHTPGHTPGSVSFHHTASHSLFDGDTLFPGGPGSTGD
                     ELGDFELIIASIRERILPMPPQTTLHPGHGDSTTVGEEAPHLEEWIARGW"
     gene            complement(2690..3349)
                     /locus_tag="VIX62_07080"
     CDS             complement(2690..3349)
                     /locus_tag="VIX62_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEY5860030.1"
                     /translation="MADLKAVMINCTLKYAPEVSNTEALMLKVKEWWDRMDVETEMVR
                     VTDYHVRFGVSSDEGNGDEWPVILEKILAADIICIGTPIWFGVRGSVAQLVIERLDGT
                     YNERNDAGQYPLYNKVGCVVVTGNEDGAHAAAETTLFNLTHLGCVIPPNADTYWVGDA
                     GPGPSYIEAGGEDHPYTQRTTRWMAHNAVHMARIMKANPIPAEGNTFDDETDHGPMHN
                     G"
     gene            3461..4729
                     /locus_tag="VIX62_07085"
     CDS             3461..4729
                     /locus_tag="VIX62_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEY5860031.1"
                     /translation="MSVELTAEDRAMLEGDRGEAPAMAMRIITAMADVSGAAHLLDIT
                     SAHIDGCLYHGPAGLDFARRLANAGGQVAVPTTLNVSSLDLLHPDLVHLDEGTRENAR
                     ALMEAHVAMDCRPTWTCAPYQLPTRPSLGEHIAWAESNAIVFANSVLGARTDRYGDFI
                     DICCAITGRAPAAGLHLDEGRAARAIFRLEGVSERLLADPIAHAAAGAVVGRATGTMV
                     PVIVGLPPAPVTTEDHAKALGAAAASSGSLAMFHAVGVTPEAPTLEAATAGLPPLLER
                     TITPDDLRDARDALSTVPVGATIGAVSVGTPHCSVREFAQLATLVDGRRMAVPFYVNT
                     GRDILAQAEREGSASVVAEAGITVVTDTCTYITPIIQARGGPVVTDSGKWAWYAPGNL
                     GLDVAFASMAECVRSAIAGELVRDEDLWGS"
     gene            4726..>4962
                     /locus_tag="VIX62_07090"
     CDS             4726..>4962
                     /locus_tag="VIX62_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEY5860032.1"
                     /translation="MSEEVRVLVGGRGLGEALVLDEPLSFWGGVDATSGAIIDTHHPQ
                     VGQSVAGRILMLHSGRGSSSSSSVLAETIRNRVGP"
BASE COUNT          718 a         1807 c         1693 g          744 t
ORIGIN      
        1 ttctcgatgc ggagggagga caacgccgcg acgccggcgg cgatcacccc gtatcgctcg
       61 cccgacgcca tgagggcatc ccagaccgcg agcccgtcat cgaacgccgc cgtcagttcc
      121 caccccagct cccccgcata cgagacgcgc aaggccgtga cggggatgcc cttcacgcgg
      181 agttcgccca cccgcaggta cccgaacgcg aggtcgccgt cggccaccgg agccaggatg
      241 tcgcgcgccg ccggtcccca caggcccagg cagcacaccg cgcccgtgag gtcggtcacc
      301 gtgacgctgc cgtcgggcgg caggtgccgg cggagccacg ccagatcccg aggaccgttg
      361 gcaccgatca cgaaccggtc ctcagccacc cgcgcgacgg tgatgtccga tcggacccca
      421 cctcgcacgt cgagcagcag cgagtaagtc accgtgccca ccggccggtc cacccggccg
      481 gccaccatcc acccgaggaa cgcctcggcc cccggcccgc tcacctcgat ccgcgtcagc
      541 gccgtcatgt cgaacagccc cgctcgctcc cgcacggcga ggtgctcggc gatcgcgatc
      601 ggggaccagt agcgcgaact ccactcgtcg cgctgcggga ccccggcggc atccgccaga
      661 cggtggttcg cctcgtacca tcgcgggcgc tcccatgccg cggtgtcgaa gaacgccgcg
      721 cccagttccc gctggcgtgg ctcgaacggg ctgaagcgga ggtcgcgcgc gctggtgtgg
      781 ccctcggcgg gatgatggac cgcgtacaca tctcgatacg agtcatcgca ccgcgcctcg
      841 aacaccgccg ggtcgagttc cggtcgctcg aagcgcgaca ggtccgccgg cgacgcgtcg
      901 atcgagggcg cgccgtcgat gagcgtctcg gcgagcacgc gtccgacgcc cgccgagtgc
      961 gtgacccaca ccgactcacc cacccagaac ccgcggaggt cgggatgctc accgatcagc
     1021 gggtacccgt cgggtgtgaa cgcgaagacc ccgttgaagc tgcgctccag catggtctcg
     1081 cgcagggcgg gcatcagctg gcacgtcaac tcccagggtt cggcgaaatc ctccggcgtg
     1141 aaggcgtaga cgatgccgtc ctcgcggcgc tcgtgggtgt cgaggccggt ggccgccacg
     1201 gggaggcggc gatggttgaa cgacccgatg ccgacgcggt cgccatggtc gcggtagtac
     1261 aaccccgcgt cgtggtggcg gacgatcggc atcgtggctt cgttccccga gttggcggcg
     1321 agcgcgggca cgggtgtcgt gacgacgtac tggtgctcga gcgccaacat cggcagtgtg
     1381 agtcccacca tcgaggcgac ggtgggcccc cacacgccgg cgcaacacac caccacgtcg
     1441 gcggggatga tctcgccgcc cacgcgaacc ccggtcacgc gaccgtcgcg gtgctcgatg
     1501 ccggtgacct cggtatcccc ctcgaacctg gcgccccggg cgatcgcgcg acgcgcctgc
     1561 gcctcgaccg ctcgcaacgc caccgcgagg ccttcaccct cggtgacgta cccgcccacg
     1621 agccccgcgg tgtcgatgat cggccatagc tgagcgatct ccgccggctc gaccatccgg
     1681 cctcggaaac cccacgcggt ggtggcgttc caccgccgct gcagctcacg cagtcgaggc
     1741 tcgttgcggg ccacctcgag ggtgcccacc ggcagcatcg ccggcgcgcc gtcgaggtcg
     1801 agcaccgtga acttgtccac cgtgtagtcc gcgagcgcct gcatcatctt ggagggactg
     1861 gtgcgcgaga ccaggcccgg ggcgtgcgag gaggatccgc ccgtcgcgta cagcggaccc
     1921 ttgtcgagca ccgtgacgtc ggtggcgccg cgcgcggtga gctcgtcggc gaccgcggcg
     1981 cccacgacgc cggcgccgat caccacgtag cgccggccgg tcaccgctca ccacccacga
     2041 gcgatccact cctcgaggtg cggagcctcc tcgcccacgg tggtcgagtc tccatggccg
     2101 gggtggaggg tggtctgggg cggcatcggc aggatccgct cgcggatcga cgcgatgatc
     2161 agctcgaagt cgccgagctc gtcgccggtg gaaccggggc cgcccgggaa cagcgtgtcg
     2221 ccgtcgaaca gcgaatgact cgccgtgtgg tggaagctga cgctcccggg ggtgtggccc
     2281 ggcgtgtgga tcacgcggag ctccacgccg ccggcgacca ggagcgcgcc ctcatccagc
     2341 gggccatccg gctggcgctc ggcgtgcgtg cgttcccaca cggggcggtc ggcgggatgc
     2401 atcaggatcg gcgcgtcgac cgcggcctgc acgtcgaacg cggcgtcgac gtggtcccag
     2461 tggccgtgcg tgcaggcgat cgccagcacc cggcgtcctc cgatcgcgtc cacgatgggc
     2521 gacgcgtcat gggaggcatc gatcaccacg acctcgtcgt cgccgcccac gatccagacg
     2581 ttgttctgga cctccacgat gccctccccg gggtagtccc gggaggcgat cgtgctcacg
     2641 aggtcgacgc gaggcggcac ggactcatcg tagccgcagg ccgcggtcct cagccgttgt
     2701 gcatcggccc gtggtcggtc tcgtcgtcga acgtgttgcc ctctgccggg atcgggttgg
     2761 ctttcatgat tcgggccatg tgcacggcgt tgtgcgccat ccacctggtg gtccgctggg
     2821 tgtagggatg atcctcgccg ccggcctcga tgtagctggg gcccgggccg gcgtcgccca
     2881 cccagtaggt gtcggcgttc ggtgggatca cgcacccgag gtgggtcagg ttgaacaagg
     2941 tggtttccgc ggcggcatga gcgccgtcct cgttgccggt caccaccacg caccccacct
     3001 tgttgtagag cgggtactgc ccggcgtcgt tgcgctcgtt gtaggtgccg tcgagccgtt
     3061 cgatcacgag ttgggccacc gagccgcgga cgccgaacca gatcggtgta ccgatgcaga
     3121 tgatgtcggc ggccaggatc ttctccagga tcaccggcca ttcgtcgccg ttgccctcgt
     3181 cggagctcac gccgaaccgc acgtggtagt cggtcacgcg gaccatctcg gtctcgacgt
     3241 ccatgcggtc ccaccactcc ttcaccttca gcatcagcgc ctcggtgttg gagacctcag
     3301 gggcgtactt cagggtgcag ttgatcatga cggccttcag gtcggccatg cgatccctcc
     3361 ttcacggggc gccggacggc gcgttctcga ccacgtctac gggcggtggg ccatcgttac
     3421 atccggtccg ccggccaatc acctgtgcca cgatgccgtg atgtccgtcg agctgaccgc
     3481 cgaggaccga gccatgctcg aaggggatcg aggcgaggcc cccgccatgg ccatgcgcat
     3541 catcaccgcg atggccgacg tcagcggtgc cgcgcacctg ctcgacatca cctccgccca
     3601 catcgacgga tgcctctacc acggcccggc gggactcgat ttcgcccgac gcctggccaa
     3661 cgcgggcggt caggtcgcgg tacccaccac actcaacgtc tcctcgctcg acctgctcca
     3721 ccccgatctc gtgcacctgg acgagggcac ccgcgagaac gcccgcgcct tgatggaggc
     3781 ccacgtggcg atggactgtc gtcccacgtg gacgtgtgcg ccctaccaac tccccacgcg
     3841 gccgtccctg ggcgaacaca tcgcgtgggc cgagtcgaac gcgatcgtgt tcgcgaactc
     3901 ggtcctgggc gcccgcaccg accgctacgg cgacttcatc gacatctgct gcgcgatcac
     3961 cggtcgcgcg ccggcggcgg gcctgcacct ggacgaggga cgggccgcgc gcgcgatctt
     4021 ccgcctcgag ggggtgtccg agcgcctgct cgccgatccg atcgcccacg ccgccgccgg
     4081 cgcggtcgtg gggcgcgcca ccggcacgat ggtcccggtg atcgtcggcc tgccgccggc
     4141 acccgtcacc accgaggatc acgccaaggc gttgggcgcc gcagccgcat cgtccgggtc
     4201 gctcgccatg ttccacgccg ttggcgtcac gccggaggcc cccaccctgg aggcggccac
     4261 ggccgggttg ccgccactgc tggagcgcac gatcacgcca gacgacctgc gggacgcgcg
     4321 cgatgcgctc tcgaccgttc ccgtgggcgc caccatcggg gcggtcagcg tcggcacccc
     4381 gcattgctcc gtgcgcgagt tcgcgcagct cgccaccttg gtcgacggcc gccgcatggc
     4441 cgtgccgttc tacgtgaaca cgggacgcga catcctcgcc caggccgagc gcgagggatc
     4501 tgcgtcggtg gtggccgaag ccggcatcac cgtggtgacc gacacgtgca cctacatcac
     4561 gccgatcatc caggcgcgcg gcggcccggt cgtcaccgat tcggggaaat gggcgtggta
     4621 tgccccgggc aacctgggcc tggacgtcgc gttcgccagc atggcggagt gcgtgcgctc
     4681 ggcgatcgcc ggcgaactcg tccgcgacga ggacctgtgg ggatcatgag cgaggaggtc
     4741 cgggtgttgg tgggcggtcg tggactcggc gaggcgctgg tcctggatga gccgctcagc
     4801 ttctggggcg gcgtcgatgc gacatccggc gcgatcatcg atacgcatca tccgcaggtc
     4861 ggacagtcgg tcgcgggccg catcctgatg ctccacagcg gccgcggatc cagctccagt
     4921 tccagcgtgc tggccgaaac gatccgcaat cgcgtcggac cg
//
LOCUS       DATDBZ010000292         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2325986, whole genome shotgun sequence.
ACCESSION   DATDBZ010000292 DATDBZ010000000
VERSION     DATDBZ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325986"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VIX62_07095"
     CDS             complement(<1..814)
                     /locus_tag="VIX62_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5860033.1"
                     /translation="MPPDALFEIARRTFDTPEFRGITCIEVEAKSVINKVPGGAMPFD
                     FTINPYRGCTHACTYCFARPTHTFLDMNAGRDFETKIVVKVNAPDVLRRELAAKRWKG
                     EHIAMGTGTDPYQRVEGRYRLMRGIIGALTEYRNPFSILTKGTLITRDLDLLTAAAEV
                     TPVSAAFSIGTLDEEVWRRTEPGTPHPKARIEAIRTLSDAGIPTSVLMAPILPGISDD
                     PVQLRAVVEAALEAGATSISPIMLHLRPGVREEFMPWLEATYPDLAPRYRQMY"
     gene            complement(882..1646)
                     /locus_tag="VIX62_07100"
     CDS             complement(882..1646)
                     /locus_tag="VIX62_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5860034.1"
                     /translation="MSRDDVRTNLTSWEAGSADYQDRNASQLNRWDRLGWGTWDIPED
                     DIGALGDVDGVEALELGTGAAQFGIKVAMRGARITGLDFSANQLGQAVANMAETGVRF
                     PLVRASAEELPFPDSSFDLVFCDHGATSFTDPHVTVAEAARVLRPGGMFVFDMATPFI
                     AIAWSEDDDEPPSRVHRRAYFDLGRSVYHDDDGDSVEWQLTYGQWIRLFRANGFAIED
                     LIELRPEPEATSTYDLAPLEWARDYPGEHIWKVRKA"
     gene            1731..>2057
                     /locus_tag="VIX62_07105"
     CDS             1731..>2057
                     /locus_tag="VIX62_07105"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY5860035.1"
                     /translation="MTEHILQLLRTAPLIDGHNDLLWALRTIRETGQDEPDLREPIPA
                     LQTDLPRIAAGGMGGQFWSVYVPSSLPEPEAVTQTLEQIDALTALCRRYPDRLEPART
                     ADDVERI"
BASE COUNT          312 a          696 c          715 g          334 t
ORIGIN      
        1 tgtacatctg tcggtagcgg ggggcgaggt cgggatacgt cgcctccagc cagggcatga
       61 actcctcgcg aacgcccggc cgcaggtgca gcatgatggg cgagatcgac gtcgcgccgg
      121 cctccagcgc ggcctcgacc accgcgcgga gctgcaccgg atcgtccgag atgcccggca
      181 ggatcggcgc catcaacacg ctggtgggga tccccgcatc ggagagcgtg cggatcgcct
      241 cgatgcgcgc cttcggatgc ggggtgcccg gctcggtgcg ccgccagacc tcctcgtcga
      301 gcgtgccgat cgagaaggcc gcgctcaccg gcgtcacctc ggcggctgcg gtcagcagat
      361 cgaggtcgcg cgtgatgagc gtgcccttcg tcaggatcga gaacgggttg cggtactcgg
      421 tgagcgctcc gatgatgccg cgcatcaacc ggtaccgccc ttcgacccgc tggtaggggt
      481 cggtgccggt gcccatggcg atgtgttcgc ccttccagcg cttggcggcc agttcgcggc
      541 ggagcacgtc gggcgcgttc accttcacca cgatcttggt ttcgaagtcg cgccccgcgt
      601 tcatgtccag gaacgtgtgg gtgggccgtg cgaagcagta cgtgcaggcg tgcgtgcacc
      661 cccgataggg gttgatcgtg aagtcgaagg gcatggcccc gcccggcacc ttgttgatca
      721 cggacttcgc ttcgacctcg atgcacgtga tgccccggaa ctccggggtg tcgaacgtgc
      781 ggcgggcgat ctcgaacagc gcatcgggcg gcatgggccc acgacgctag aacatatgtt
      841 cgattccggc aagcggcggc ggagcctcgg ttcggcccgc gtcaggcctt ccggaccttc
      901 cagatgtgct cgcccgggta gtcacgggcc cactccaagg gggcgagatc gtaggtgctg
      961 gtggcctccg gttcgggccg cagctcgatc agatcctcga tcgcgaaccc gttggcgcgg
     1021 aacaggcgga tccactggcc gtaggtgagt tgccactcga cgctgtcacc gtcgtcgtcg
     1081 tggtagacag accggcccag gtcgaagtac gcacgccgat gcacgcggct cggcggttcg
     1141 tcgtcgtcct cgctccacgc gatcgcgatg aacggtgtcg ccatgtcgaa cacgaacatc
     1201 ccgccgggac gcagcacgcg ggcggcctct gccacggtga cgtgcgggtc ggtgaacgag
     1261 gtggcgccgt gatcgcagaa caccaggtcg aacgaggaat ccgggaaggg cagctcctcg
     1321 gcgctggcgc gcaccagcgg gaaccggacg ccggtctcgg ccatgttcgc gaccgcctgc
     1381 ccgagctggt tcgcgctgaa gtccaggccg gtgatccggg caccgcgcat cgccaccttg
     1441 atgccgaact gcgcggcgcc ggtgcccaac tcgagcgcct cgaccccgtc cacgtcgccc
     1501 agcgcgccga tgtcgtcttc cgggatgtcc caggtgcccc agccgagccg gtcccaccgg
     1561 ttgagctgcg aggcgttgcg atcttggtag tcggcgctgc cggcttccca gctggtgagg
     1621 ttcgtccgaa cgtcgtcgcg actcatggct cgatgtctac cacggcaggt tggttcggcg
     1681 cgggtggccg acccatcgcc gggtccgagc caacgcctag gatgcgggcc atgaccgagc
     1741 acatcctgca gctgctgcga accgctccct tgatcgacgg acacaacgac ctgttgtggg
     1801 cattgcgcac gatccgcgaa acgggtcagg acgagccgga tctccgggag cccataccgg
     1861 ccctgcagac cgacctgccg cggatcgccg ccggcgggat gggtgggcag ttctggtcgg
     1921 tctacgtgcc gtcgagcctg cccgagcccg aagccgtgac gcagacactc gagcagatcg
     1981 atgcgctgac cgccctgtgc cgtcgctacc cggaccggct cgaaccggcg cgcaccgccg
     2041 acgacgtcga gcggatc
//
LOCUS       DATDBZ010000293         8069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1103708, whole genome shotgun sequence.
ACCESSION   DATDBZ010000293 DATDBZ010000000
VERSION     DATDBZ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103708"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /gene="tsaE"
                     /locus_tag="VIX62_07110"
     CDS             <1..482
                     /gene="tsaE"
                     /locus_tag="VIX62_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY5860036.1"
                     /translation="LRAPTAEDTREVGEALSASLRARDAVVLTGELGAGKTTFVQGVA
                     RGLGIEDQVSSPTFTLVKEYRGILDIAHVDVYRLERVQDVVDLGLDELGDGEGVLLVE
                     WGDAVEDLLPDERLRVELTTEDLVSDVRRLRVSALGASWQERWGALEVAVAPWSVAS"
     gene            479..1186
                     /gene="tsaB"
                     /locus_tag="VIX62_07115"
     CDS             479..1186
                     /gene="tsaB"
                     /locus_tag="VIX62_07115"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY5860037.1"
                     /translation="MIVLGIETSTPQTSIAIGTETEILAKVSIAGRARQEVVAPALDQ
                     LLRWTDLDLSRVGGIAVGIGPGLFTGLRVGVETAKTLAQVLHVPIVGITSLDALAYAV
                     RTSHKRIAAVIDGRRGEVFFAVYRSVPGGVVRETEQVVITPDRLVAELEALGGEVLAV
                     GNGAMLYRHGLEELGSQIEIASAVVAHPEAASLVELAVPRFLREEHDRLYDVVPVYLR
                     KSDAEIAWDQRARGATG"
     gene            1197..1691
                     /gene="rimI"
                     /locus_tag="VIX62_07120"
     CDS             1197..1691
                     /gene="rimI"
                     /locus_tag="VIX62_07120"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY5860038.1"
                     /translation="MRRRHLRGVMAIERQVYARPWSPNLFVAEMTEPNNRCYLVARID
                     KTVVGYGGLICYGDEAHVTNIAVDPQMHRHKIGTRVLHELMVQSIELGARAVSLEVRV
                     TNWGAQRLYGRYGFHPVGIRRNYYQELNEDALIMWTDDVRTPDYGRRLASIVAELPET
                     VRPS"
     gene            1688..2695
                     /gene="tsaD"
                     /locus_tag="VIX62_07125"
     CDS             1688..2695
                     /gene="tsaD"
                     /locus_tag="VIX62_07125"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY5860039.1"
                     /translation="MITLGIETSCDETAVAVVEDGFTLRANLIASQVHLHERFGGVVP
                     EVAARAHVEALNPLMEEALGEAEIGFGDIDAVAVTVGPGLVGALLVGMAAAKGVALAT
                     GADLVGVNHLEAHYWANFLEHGTPEPPYVALIVSGGHTMLVHVPTMLHHEVLGQTLDD
                     AAGEAFDKVARLLGLGFPGGPALDAMATDGDPNAIHFPRAMEDSGDYDFSMSGLKTAV
                     LRFVQRERAAGHDLDLPDLAASFQEAIVDVQVSKAMAAAKDVGAPTVLMGGGVVANSR
                     LRTRLAEAGEREGIRVLSPSLPLCTDNAAMVACVGTARWKAGERTVLDIGADPQLRLA
                     P"
     gene            2692..3636
                     /locus_tag="VIX62_07130"
     CDS             2692..3636
                     /locus_tag="VIX62_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5860040.1"
                     /translation="MSTVLVTGGAGFIGSHLADRLLAEGHRVISVDDLTTGRIANLVD
                     ARGYGKEFTFFNMDVRADGLSSLFERHRPEVVFHLAAQSGVRPSLADPVLDASINVMG
                     TMNVLECAAKVEVRKVIYAASGGTIYGEPKRIPAKETAAQASHPLSPYGISKKVVMDY
                     LGFFQRYRGLDYTALALGNVYGPRQDPHGEAGVIAIFAQKMLAGEQVTIFGDGNQTRD
                     YVFIDDVAHAFVQAMDRGSGKLVNVGTGLEASVNHLYRLLADIIGYDKDPEFGTLPPG
                     ELRRIALDIGKAPSAIAWKPWTHLEDGLAETVAFLKGV"
     gene            3657..4037
                     /locus_tag="VIX62_07135"
     CDS             3657..4037
                     /locus_tag="VIX62_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017599740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5860041.1"
                     /translation="MDDLPVSDPTFSLHVVDVPLEPGELDPASILAGSPEVTETVLWE
                     SPDGRIVRGIWRITQGTVTDTEQDELFVVIEGRATIEVADGPTIEVGPGDVCILERGA
                     RTTWTVHEPLRKVFQITLDAPERA"
     gene            4043..5452
                     /locus_tag="VIX62_07140"
     CDS             4043..5452
                     /locus_tag="VIX62_07140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5860042.1"
                     /translation="MAISTERARASYADAEPTPFWLDRPDAPSPCSPLDGPATADLAI
                     VGGGYTGLWTAIHAKEEDPGRDVVLVERDTIAFGASGRNGGFCDATLTHGLENGLSRF
                     PDEIDRIEAVGRESFRGLTETIARHGIECDWVTSGEVGVATEPHQVDWLREAAHHGRA
                     RGLDSVFLDRDQVRAKLNSPSILAGVWKRDHCAMVDPARLAWGLKRVALELGVRIHET
                     TPVTDLASTGSGVRLGAPAGHIDARRVVLGTNGFTPLVRSIRRYVVPVYDYVLVTEPL
                     SEVQRDAIGWAERQGFADSTNHFHYFRTTEDGRILWGGYDAIYHFANGVRPEFEQRDA
                     TFSLLARQFFDMFPQLEGLRFSHRWGGVIDTCSRFSVMFGTALDGKVAYAVGYTGMGV
                     AATRFGARTALDLVDGRDTERTRLRLVRSKPIPFPPEPLRWAGIHLTTRALTRADERE
                     GRRGPWLRLLDSVGLGFDS"
     gene            5591..5887
                     /gene="groES"
                     /locus_tag="VIX62_07145"
     CDS             5591..5887
                     /gene="groES"
                     /locus_tag="VIX62_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY5860043.1"
                     /translation="MADFNLKPLEDRIVVQASEGDQTTASGLVIPDTAKEKPQEGSVV
                     AVGPGRFEDGARVPLDVAVGDKVIYSKYGGTEVKVEGQEYLILSARDVLAVLSK"
     gene            5978..7588
                     /gene="groL"
                     /locus_tag="VIX62_07150"
     CDS             5978..7588
                     /gene="groL"
                     /locus_tag="VIX62_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5860044.1"
                     /translation="MASKLLKFGEEARRGLEAGVDKLADAVAITLGPKGQNVVLDKKW
                     GAPTITNDGVTIAKEIELDDPWENMGAQLTKEVATKTNDVAGDGTTTATVLARAMVKG
                     GMKNVAAGANPMALKRGIEKAVELAVEAIKNQAKEVESKEEIAQVASISAADPAIGET
                     IAEALDKVGKDGVITVEESNTFGIELEFTEGMQFDKGYISPYFISDAEAQEAVMDDPA
                     ILVVNKKISSVQELLPVLEKVMQGGKPLVIIAEDVEGEALATLVVNKIRGTFNAAAVK
                     APGFGDRRKAMLQDIAILTGAEVISEEVGLKLENADLNLLGSARRVIITKDTTTIVEG
                     QGDAEAVSGRIAQIKAEVENTDSDWDREKLQERLAKLSGGVAVIKVGAATEVELKEKK
                     HRIEDAVSATRAAVEEGIVPGGGVTLVRAESALDKVDLTDDEATGANIVRGALAEPAR
                     RIAQNAGYEGSVIVAQLRSEGEGRGFNAATGEWVDMVKTGIIDPAKVTRSALQNAASI
                     AAIVLTVESAVVEKPEEEEAAAGDGHMH"
     gene            7812..>8069
                     /locus_tag="VIX62_07155"
     CDS             7812..>8069
                     /locus_tag="VIX62_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY5860045.1"
                     /translation="MFVGVHSTVLGPALGGCRMSAYESVADATRDALRLARGMTYKSA
                     AAGLPLGGGKSVIAMQPGVDREAMLLDLADSVESLDGLYVAA"
BASE COUNT         1338 a         2667 c         2831 g         1233 t
ORIGIN      
        1 agcttcgggc cccgacggcc gaggacaccc gcgaggtggg agaggcgttg tcggcctcct
       61 tgcgcgcccg cgacgccgtc gtgctgaccg gcgagctggg ggccggcaag accaccttcg
      121 tgcaaggcgt ggcgcgcgga ctcgggatcg aggaccaggt gtcatcgccc acgttcacgc
      181 tggtgaagga gtaccggggc atcctcgaca tcgctcacgt cgacgtctac cggttggaac
      241 gggtgcagga cgtggtcgac ctcgggctgg atgagctggg cgacggtgaa ggggtgctcc
      301 tcgtggaatg gggcgacgcc gtcgaggacc tgctgcccga cgagaggctc cgcgtcgagc
      361 tcaccaccga agacctggta tcggatgtgc ggcggctccg cgtctcggcg ctcggcgcct
      421 cgtggcagga gcggtggggt gcgctcgaag tggccgtggc cccctggagc gtggcgtcgt
      481 gatcgtcctg gggatcgaga catccacccc gcagacgagc atcgcgatcg gcaccgagac
      541 cgagatcctc gcgaaggtct cgatcgccgg ccgggcccgg caggaggtgg tggcgccggc
      601 gctggaccag ctcctgcgct ggaccgatct cgacctgtcg agggtgggcg ggatcgccgt
      661 ggggatcggt ccgggcctgt tcaccggcct gcgggtcggc gtcgaaaccg ccaagaccct
      721 tgcacaggtg ctgcacgtgc cgatcgtggg gatcaccagc ctcgacgcgc tcgcctacgc
      781 ggtccgcacc tcgcacaaac ggatcgcggc ggtgatcgac ggccggcgcg gcgaggtctt
      841 cttcgcggtg taccgatcgg taccgggcgg ggtcgtccgc gagaccgagc aggtcgtcat
      901 caccccggac cggctcgtcg ccgaactcga agcgctcggt ggagaggtgt tggcggtggg
      961 caacggtgcg atgctgtatc gacatggact ggaagagctc ggaagccaga tcgagatcgc
     1021 ctcggcggtc gtcgcccatc ccgaggcggc gtccttggtc gaactcgccg ttccccggtt
     1081 cctccgggag gagcacgatc gtctctacga cgtggtcccg gtctacctga ggaagtccga
     1141 tgctgagatc gcgtgggatc agcgagccag gggcgccacg ggttgaggtc agccgcatgc
     1201 gccggcgcca cctgcgtggc gtcatggcga tcgagcggca ggtctacgcc cgtccctgga
     1261 gtcccaacct cttcgtcgcc gagatgaccg agcccaacaa ccggtgctac ctcgtggcgc
     1321 ggatcgacaa gaccgtggtc ggctacggcg gtctgatctg ctacggcgac gaggcccacg
     1381 tcacgaacat cgccgtcgac ccgcagatgc accggcacaa gatcgggacg cgggtgctcc
     1441 acgagctgat ggtccagtcg atcgaactcg gggctcgcgc cgtgagcctg gaagtccgtg
     1501 tcaccaactg gggcgcccaa cggctctacg ggcgctacgg cttccatccg gtggggatcc
     1561 ggcgcaacta ctaccaggag ctcaacgagg acgccttgat catgtggacc gacgacgtcc
     1621 ggacgcccga ctacggtcgg cggctggcct cgatcgtggc cgagttgccg gagacggtgc
     1681 gcccctcgtg atcaccctgg gtatcgagac ctcctgcgac gaaaccgccg tcgccgtggt
     1741 ggaggacggt ttcacgctgc gcgccaacct gatcgcctcc caggtccacc tgcacgaacg
     1801 cttcggtggg gtggtgccgg aggtggcggc gcgagcccat gtcgaggcct tgaacccgct
     1861 gatggaagag gcgcttggcg aggccgagat cgggttcgga gatatcgacg cggtcgccgt
     1921 caccgtgggt cccggcctgg tgggagccct gctggtgggc atggcagcgg ccaagggcgt
     1981 ggccctcgcc accggggccg acctggttgg ggtgaaccac ctcgaggcgc actactgggc
     2041 gaacttcctg gagcacggaa ctcccgagcc gccgtacgtg gcgctgatcg tctccggcgg
     2101 acacacgatg ttggtgcacg taccgacgat gctgcatcac gaggtcctgg gccagacgct
     2161 cgacgatgcc gcgggcgagg ccttcgacaa ggtggcgcga ctgctgggac tcgggttccc
     2221 cggaggaccg gcgctcgacg cgatggcgac cgacggtgat ccgaacgcga tccacttccc
     2281 gcgcgcgatg gaggattccg gcgactacga cttctcgatg agcggactca agaccgccgt
     2341 gctccgattc gtgcaacggg aacgggcggc cggccacgac cttgacctgc ccgatctcgc
     2401 cgcgagcttc caggaagcga tcgtcgacgt gcaggtgtcc aaagccatgg ccgccgccaa
     2461 agacgtgggc gcgccgaccg tgttgatggg tggaggcgtg gtcgccaact cgcgtctgcg
     2521 cacgcggttg gccgaggcgg gcgaacgcga aggcatccgt gtgctcagcc cgtcgttgcc
     2581 cctgtgcacg gacaacgcgg cgatggtggc gtgcgtgggg acggcacgat ggaaggccgg
     2641 agagcgcacg gtgctcgaca tcggcgccga cccgcagcta aggttggcgc cgtgagcacg
     2701 gtcctggtga ccgggggcgc cgggttcatc ggatcgcacc tcgccgacag actcctcgca
     2761 gagggacacc gcgtcatcag cgtggacgac ctcaccaccg gtcgcatcgc gaacctggtg
     2821 gacgcgcgag gctacggcaa ggaattcacg ttcttcaaca tggacgtgcg cgccgatggt
     2881 ctgtcgtccc tgttcgagcg gcatcggccg gaggtggtct tccacctcgc ggcccagtcc
     2941 ggcgtgcgcc catcgctcgc cgatcccgtg ctcgatgcgt ccatcaacgt gatgggcacg
     3001 atgaacgtgt tggagtgcgc cgccaaagtc gaggtgcgca aggtgatcta cgccgcgagc
     3061 ggcggaacga tctacggcga acccaagcgc atccccgcca aggagaccgc cgcccaggcc
     3121 tcgcatccgc tcagcccgta cggcatctcg aagaaggtgg tcatggacta cctggggttc
     3181 ttccagcgct atcgcggcct cgattacacg gcgttggccc tgggcaacgt gtacggcccc
     3241 cggcaggatc cccacgggga agcgggggtg atcgccatct tcgctcagaa gatgctggcc
     3301 ggcgaacagg tcacgatctt cggcgacggg aaccagacgc gcgactacgt gttcatcgac
     3361 gacgtggcgc acgccttcgt ccaagcgatg gatcgaggct cgggcaagct cgtgaacgtc
     3421 ggcaccggcc tggaggcgag cgtcaaccac ctgtaccggc tgctcgccga catcatcggc
     3481 tacgacaagg atcccgagtt cggcacgctg ccgccggggg agcttcgccg gatcgcgctc
     3541 gacatcggga aggcccccag cgccatcgcg tggaagccgt ggacccatct cgaggacggg
     3601 ctcgccgaga ccgtcgcgtt cctcaagggc gtgtgagcgc ggtagcctct cccgcgatgg
     3661 acgacctgcc cgtgtccgat ccgacgttct ccctgcacgt cgtcgacgtt cccctcgaac
     3721 ctggggaact cgatccggcg tcgatcctgg cggggtcgcc ggaggtcacc gagaccgtgc
     3781 tgtgggagtc gcccgacggc cggatcgtcc gcggcatctg gcgcatcacg cagggcacgg
     3841 tcaccgacac cgagcaggac gagctcttcg tggtgatcga ggggcgagcc acgatcgagg
     3901 tcgccgacgg tcccacgatc gaggtgggtc cgggcgacgt ctgcatcctc gaacgcggcg
     3961 cgcgcaccac gtggacggtc cacgaaccgc tccgcaaggt gttccagatc accctggacg
     4021 cgccggaacg agcctgagcc acgtggcgat cagcaccgag cgagcccgcg cgagctacgc
     4081 cgacgccgag ccgaccccgt tctggctgga tcggccggat gcaccgtctc cctgttcccc
     4141 gctcgacggc cccgcgacgg ccgacctcgc gatcgtgggc ggcggttaca ccggcctgtg
     4201 gacggcgatc catgccaagg aagaggatcc cgggcgcgac gtcgtcctgg tcgaacgcga
     4261 cacgatcgcg ttcggcgcca gcgggcgaaa cggcgggttc tgcgatgcca cgctgaccca
     4321 tggactcgag aacggcctgt cccgtttccc cgacgagatc gaccggatcg aagcggtggg
     4381 ccgcgagagc ttccgtggcc tgaccgagac gatcgcgcgc cacggcatcg agtgcgactg
     4441 ggtgaccagc ggggaggtcg gcgttgccac cgaaccgcat caggtcgact ggctccgcga
     4501 ggccgcccac cacgggcggg cccgcggtct cgacagcgtc ttcctggatc gggaccaggt
     4561 ccgggccaag ctgaactccc catcgatcct ggccggtgtc tggaagcgtg accattgcgc
     4621 catggtcgac cccgcacgcc tggcatgggg gttgaagcgc gtggcgctgg aactcggcgt
     4681 ccgcatccac gaaaccacgc ccgtcaccga tctcgcgtcg accggttcag gtgtgcggct
     4741 gggtgcgccg gcgggtcaca tcgacgcgcg acgggtcgtg ctgggtacga acgggttcac
     4801 gccgctggtg cgctcgatcc gccggtacgt ggtgcccgtc tacgactacg tgctcgtcac
     4861 cgagcccctc tccgaggttc agcgggacgc gatcggatgg gctgaacggc aggggttcgc
     4921 cgattccacc aaccatttcc actacttccg caccaccgag gacggccgga tcctgtgggg
     4981 cggctacgac gcgatctacc acttcgcgaa tggcgtgcgg cccgagttcg aacagcgcga
     5041 cgccacgttc tcgttgctgg cccggcagtt cttcgacatg ttcccgcaac tcgaagggct
     5101 gcggttctcg catcgatggg gtggcgtgat cgacacctgc agtcggttct cggtgatgtt
     5161 cggcacggcg ctggacggga aggtcgccta cgcggtcgga tacaccggca tgggcgtggc
     5221 ggccacgcgg ttcggtgccc gcacggcgct ggacctcgtg gacggccgtg acaccgagcg
     5281 cacccgcttg cggctcgtgc ggtccaagcc gatcccgttc cctcccgaac cgctgcgctg
     5341 ggccggcatc catctcacga ccagggcgct cacccgggcg gatgagcgcg aaggccgtcg
     5401 cggtccctgg ctccggctgc tcgactcggt gggcctgggc ttcgacagct gaaccgatcg
     5461 ctccctggtc ccctcgctcg gtcgcgttga ccctctggca ctcgcctggg tatgctgtta
     5521 gcactcgtga ccatcgagtg ctaatcacac atctcggtca acgccgatca gcacaaggag
     5581 gaaggcacag atggcggatt tcaacctcaa gcctctcgag gaccgcatcg tcgttcaagc
     5641 cagcgagggt gaccagacca ccgcgtccgg cttggtgatc cccgacaccg cgaaggagaa
     5701 gccccaggag ggttccgtcg tggcggtggg ccccggtcgc ttcgaggacg gcgctcgtgt
     5761 gccgctcgac gtcgcggtgg gcgacaaggt gatctattcg aagtacggcg gcaccgaggt
     5821 caaggtcgag ggccaggagt acctgatcct gagcgcccgc gacgtgctgg ccgtgctctc
     5881 caagtaacca acctgctctg cggtaccggg gttcgcacgc gctgcgcacg caggcggcgg
     5941 accccgttcc cgtctctacg acctgaagga ggtaccagtg gcttcgaagc tgctgaagtt
     6001 cggcgaggaa gcgcgccgtg gcctggaggc aggcgtcgac aagctcgccg acgccgtcgc
     6061 catcacgctc ggccccaagg gccagaacgt ggtgctcgac aagaagtggg gagcccccac
     6121 gatcacgaac gacggcgtca cgatcgccaa ggagatcgag ctcgacgacc cgtgggagaa
     6181 catgggcgcc cagctcacca aggaggtcgc caccaagacg aacgacgtcg ccggtgacgg
     6241 caccaccacg gccaccgtgc tcgcccgcgc catggtcaag ggcggcatga agaacgtggc
     6301 tgccggcgcc aacccgatgg cgctgaagcg cggcatcgag aaggcggtcg agctggccgt
     6361 cgaggccatc aagaaccagg ccaaggaagt ggagtccaag gaagagatcg cccaggtggc
     6421 gtccatctcc gccgccgacc ccgcgatcgg cgagaccatc gccgaggccc tggacaaggt
     6481 gggcaaggac ggcgtcatca cggtcgagga gtcgaacacc ttcggcatcg agctcgagtt
     6541 caccgagggc atgcagttcg acaagggcta catctcgccc tacttcatct ccgacgccga
     6601 agcgcaggaa gcggtcatgg acgacccggc catcctggtc gtcaacaaga agatctcgag
     6661 cgtgcaggag ttgctgccgg tgctcgagaa ggtgatgcag ggcggcaagc cgctcgtgat
     6721 catcgccgag gacgtcgagg gcgaagccct ggcaacgctg gtggtcaaca agatccgtgg
     6781 cacgttcaac gccgccgcgg tgaaggctcc agggttcggt gaccgtcgca aggccatgct
     6841 gcaggacatc gcgatcctga ccggtgccga ggtcatctcc gaggaggtcg gtctcaagct
     6901 cgagaacgcc gacctcaacc tgctcggctc ggctcgccgg gtcatcatca cgaaggacac
     6961 cacgacgatc gtcgagggtc agggtgacgc cgaggccgtg agcggccgca tcgcccagat
     7021 caaggccgag gtcgagaaca ccgactccga ctgggaccgc gagaagctcc aggagcgcct
     7081 tgccaagctg tccggtggcg tcgcggtgat caaggtcggc gcggccaccg aggtcgagct
     7141 caaggagaag aagcatcgca tcgaggacgc cgtctccgcg acccgcgcgg ccgtggagga
     7201 aggcatcgtc cccggcggcg gtgtgaccct ggtccgtgcc gagtcggcac tggacaaggt
     7261 cgacctcacc gacgacgagg ccacgggcgc gaacatcgtc cggggcgcac tcgccgagcc
     7321 tgcgcgtcgc atcgcgcaga acgccggcta cgagggttcg gtcatcgtcg cgcagctccg
     7381 cagcgagggt gagggccgag gcttcaatgc cgcgaccggc gagtgggtcg acatggtcaa
     7441 gaccggcatc atcgacccgg ccaaggtgac gcgttcggcg ctgcagaacg cagcctcgat
     7501 cgccgccatc gtgctgacgg tcgagagcgc cgtggtcgag aagcccgagg aagaggaagc
     7561 ggcggccggc gacggccaca tgcactaagg ctcgctcact cgagcccgct ccacgttgag
     7621 aggggccgag gcccaccgcg caggggaaac tgaacgcccc ttcgcggtga cctcggcccc
     7681 tttcgcgttc ccggcccgac ctcgttcggg tagttcagga cttagactgc ggcgcgatgt
     7741 tcgagggacc cttgcgggat tgggatgggg aagaggtcgt tcttcggttc gatgcctcga
     7801 ccggcgcgtg gatgttcgtc ggggtgcatt cgacggtgct cgggccggcg ctcggcggct
     7861 gccgcatgtc cgcctacgag agcgtggcgg acgcgacgcg cgacgcgctg cggctcgcgc
     7921 gcggcatgac ctacaagtcg gccgcggcgg ggctgccgct cggcggcggc aagagcgtga
     7981 tcgcgatgca gccgggtgtg gaccgcgagg caatgctgct cgacctcgcc gacagcgtcg
     8041 aatcgctcga cgggctctac gtcgccgcc
//
LOCUS       DATDBZ010000294         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_308227, whole genome shotgun sequence.
ACCESSION   DATDBZ010000294 DATDBZ010000000
VERSION     DATDBZ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308227"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VIX62_07160"
     CDS             complement(<1..692)
                     /locus_tag="VIX62_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEY5860046.1"
                     /translation="MPELETRAESDDRDRSASDPERGLTASEVAERVDRGQINDVPAA
                     PTRTVSQIVRGNIFTRFNAILGAMLVVILIVGPFQDALFGFVLIANAAIGIYQELRAK
                     QTLDSLTVLTAPKARAVRDGEVQEVAVGELVLDDVLDVTAGSEIVVDGVVLASAGMEV
                     DESLLTGESEPVVKAPGDDVLSGSFIAAGSGRYRASKVGKEAYAVQLAQDARRFTLTR
                     SELRSGIDRILT"
     gene            725..1930
                     /locus_tag="VIX62_07165"
     CDS             725..1930
                     /locus_tag="VIX62_07165"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY5860047.1"
                     /translation="MKNPGWIRPAPMREFDVEDRRSTWLELFFDLCFVAAIAALADGL
                     HHDPTMQGLWHFALLFVPVWWAWMEFTWFATAWDNDDLFHRLAMLLAMLLVIVMAAGI
                     ASLLKGKGDSLFVWSYAGLQLLLIVMFVRVWPHAGRARGLVRDYMIGDAIGAVLMVGS
                     LFVDPPLRYWIWAAAVAVLMIAPAFAVRAYEGQPFDSRHIPERYGLFTIIVLGEGVVA
                     VTAGLSDVSLDGGSVASAVLGFGIAAAIWYAYFETVSSSALSRERVGASFLWGYGHLF
                     AFAGIAAAAVGVELAIEAGAHGDHGLSLATRLMVCAGPAAFLLALAAIHKTTIRTWDT
                     VMTQRMGAIAVLLGVAAFGRGLGPAFLVGIVFVVLTVTVAFDVVRAGGKGLAAEDAPG
                     DETHVPGPV"
     gene            complement(1936..>2444)
                     /locus_tag="VIX62_07170"
     CDS             complement(1936..>2444)
                     /locus_tag="VIX62_07170"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the hydrolysis of a monocarboxylic acid
                     amid to form a monocarboxylate and ammonia; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5860048.1"
                     /translation="ELSGTSIRTLADVVAFNEAHPEEELAIHSQGTLEGALGEAPLND
                     PGYARSLRLSRRLGRAAFDEPMKAHRLDAIVAPSFRPSWLIDLRRGDGPGHGNGAAGP
                     SNAAGYPHLTVPAGFVGELPVGISFMARAWEEPKLLAYGYAFEQATHARRIPKFLRLY
                     NAEAFVER"
BASE COUNT          341 a          793 c          878 g          432 t
ORIGIN      
        1 taggtgagga tgcggtcgat gcccgagcgc agttccgagc gggtgagcgt gaaccggcgc
       61 gcgtcctgcg caagctgcac cgcgtaggcc tccttgccca ccttgctcgc gcggtagcgc
      121 cccgaaccgg cagcgatgaa cgaccccgac aacacgtcgt cgccgggggc cttgaccact
      181 ggttcggatt caccggtcag gagtgattcg tcgacctcca tgccggcgct cgcgagcacg
      241 acgccgtcca ccacgatctc ggaccctgcc gtgacgtcga gaacgtcgtc caggacgagc
      301 tcgccgacgg cgacctcctg aacctcgccg tcacggacgg ctcgcgcttt cggcgcggtg
      361 agaacggtga gcgagtccag ggtctgcttc gcccgcaact cctggtagat cccgatcgcc
      421 gcgttcgcga tcagcacgaa cccgaacaac gcgtcctgga acgggcccac gatcaggatc
      481 accacgagca tcgcgcccaa gatcgcgttg aaccgcgtga agatgttgcc gcgcacgatc
      541 tgggagaccg tgcgggtggg agccgcgggg acgtcgttga tctggccgcg gtcgacccgc
      601 tcggcgacct cggacgccgt cagtcctcgt tcgggatcgg aggccgatcg gtcacggtcg
      661 tcggattcag cgcgggtctc gagctcgggc atcggcccga gcctagcggg ctacgctggg
      721 acgcatgaag aacccagggt ggatccggcc ggctcccatg cgcgagttcg acgtcgagga
      781 ccgacgatcg acctggttgg agctgttctt cgacctgtgc ttcgtggccg cgatcgcggc
      841 gctcgccgac gggttgcacc acgacccgac gatgcagggc ctgtggcact tcgcgctgct
      901 gttcgtgccg gtgtggtggg cgtggatgga gttcacctgg ttcgcgaccg cgtgggacaa
      961 cgacgacctg ttccaccggc tcgccatgct gctcgcgatg ctgttggtca tcgtgatggc
     1021 cgccggcatc gcctccctgc tcaaaggcaa gggcgactcg ctgttcgtat ggtcctacgc
     1081 gggactgcag ttgctgttga tcgtgatgtt cgtgcgggtg tggccgcatg cgggacgagc
     1141 gcgtgggctc gtgcgcgatt acatgatcgg cgacgcgatc ggcgcggtgc tgatggtggg
     1201 ctcgctgttc gtcgatccgc cgctccgcta ttggatctgg gccgccgccg tcgcggtgct
     1261 gatgatcgcg ccggcgttcg cggtgcgtgc ctacgagggc cagccgttcg actcccggca
     1321 catccccgag cgctacggcc tgttcacgat catcgtgctg ggtgaagggg tggtcgcggt
     1381 cacggcaggc ctctcggacg tgtcacttga cggcggttcg gtcgcgtcgg cggtgctggg
     1441 gttcgggatc gcggcggcga tctggtacgc ctacttcgag acggtgtcgt cgtcggcgct
     1501 ctcgcgtgag cgcgtgggag cgtcgttcct gtgggggtac gggcacttgt tcgccttcgc
     1561 gggcatcgcg gccgccgccg tcggagtgga actggccatc gaggcggggg cgcacggcga
     1621 tcacgggctg tccttggcaa cccggctgat ggtgtgcgcc gggcccgccg cgttcctgct
     1681 ggcgctcgcc gcgatccaca agacgaccat ccggacgtgg gacaccgtca tgacccagcg
     1741 gatgggggcg atcgcggtgc tgctcggcgt ggcggcgttc gggcgggggc tcggcccggc
     1801 gttcctggtg ggcatcgtgt tcgtggtact cacggtgacg gtggccttcg acgtcgtgcg
     1861 cgccggcggc aagggcctgg ccgccgagga cgcacccggc gacgagaccc acgtaccggg
     1921 ccccgtgtag tgggcctacc gttccacgaa ggcttccgcg ttgtacaggc gaaggaactt
     1981 cgggatgcgc cgggcatggg tggcctgttc gaacgcgtag ccgtacgcca gcaacttggg
     2041 ctcctcccat gcgcgggcca tgaacgagat gccgaccggg agttcaccca cgaagccggc
     2101 tgggaccgtc aggtgcggat accccgcggc gttcgagggg cccgccgctc cgttcccgtg
     2161 tcctggaccg tcgcctcgcc gcaggtcgat gagccacgaa gggcggaaac tgggcgccac
     2221 gatggcatcc aggcggtgag ccttcatggg ttcgtcgaag gcggctcggc ccaaacgacg
     2281 cgacaagcgt agtgaccggg cgtagcccgg atcgttgagc ggcgcctcgc cgagcgcccc
     2341 ctcgagcgtg ccttgtgaat ggatcgcgag ctcttcctcc gggtgcgcct cgttgaacgc
     2401 gacaacgtcg gccaaggtcc tgatcgatgt acccgacaac tctg
//
LOCUS       DATDBZ010000295         6936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1940937, whole genome shotgun sequence.
ACCESSION   DATDBZ010000295 DATDBZ010000000
VERSION     DATDBZ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940937"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..463)
                     /locus_tag="VIX62_07175"
     CDS             complement(11..463)
                     /locus_tag="VIX62_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY5860049.1"
                     /translation="MSTDFVPDGFTPPDGLVAEAFVLEPLGPQHNEADHAAWMSSIEH
                     IHASPGFSDDFDDDPDPWPRPMSLEENLSDLDQHAKDFQDRVGFTYTVLDPADRDVIG
                     CVYLYPSPLDGGQRLLIMDLQEPRSAAFVSIVVVHGWTLPSRVGPVLR"
     gene            515..880
                     /locus_tag="VIX62_07180"
     CDS             515..880
                     /locus_tag="VIX62_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5860050.1"
                     /translation="MSDDDRYERGRARFLEVHDEKALAAVEGLGDLGRMIVEVVYGDV
                     YGRSELSTRDRELVTVAALVSLGRSAQLPQHLRASLKAGLTPQELGEVIMQTAVIAGF
                     PPAMNAMSTLRTIVAERDR"
     gene            complement(888..1622)
                     /locus_tag="VIX62_07185"
     CDS             complement(888..1622)
                     /locus_tag="VIX62_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860051.1"
                     /translation="MAQTLALAMLVFVIAAGAGLLGAMGRLDRPSASAPSPVPVIDED
                     DAVGLLRVRCIERGAGIEVPAVNAKADGVHVAIEGTEGQKVFFQSDAGLVYPMQLREN
                     GGTFELPLPPGSWHATCAPEGSSAPAPSSGAGFVVTDPASRYVSYQPECPVDETGQQL
                     NGELPTSFVQDQDTAIREVLADDGLLEGDLIERAGYRTSIPGQDPPNPMAFRVMRGDT
                     VVGAFEAMEGPDGWTYFMGGCLNSEG"
     gene            complement(1703..2305)
                     /locus_tag="VIX62_07190"
     CDS             complement(1703..2305)
                     /locus_tag="VIX62_07190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5860052.1"
                     /translation="MVSYPADRGFDDSYLGVPPWDIGRPQREVEALIAAGRFEGPVLD
                     AGCGTGENALAIARQGEAVLGIDASPRAIGKAIDKARERGLERAEFLVADVFALEGLG
                     RTFDSALDCGLFHVFDDHERPVYAASLAGVVNPGGTLHLLCFSEREPLKWGPRRVTEA
                     ELRATFDDGWSVTEIVPAVFETNRDEGDVRAWRATIRRTG"
     gene            2379..3239
                     /locus_tag="VIX62_07195"
     CDS             2379..3239
                     /locus_tag="VIX62_07195"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY5860053.1"
                     /translation="MTLFRLRDDVPTLVAPVLVGAFDGWIDAGGAASAAAERLAEGAE
                     TIATIDPDAVFDYRSRRPVLDVVDGTLTHLDWPEISVGRRTIDGRDVLVLRGPEPDFR
                     WRELAASASEIAVRLGALQWISLGAIPAAVPHTRAVSVFGTASADGLLADDVEQGPAG
                     LLRVPAAALSVVEMSMQSSGLPTVGFYAQVPHYVGGPFAPGAIALLAQLDRHLGIEVP
                     LGELAETAIDQRTRLDAAVDADEDARSYLDRLESMTGSEGVPTGDELATEIERFLQGE
                     AGGEEPPRLT"
     gene            complement(3253..4950)
                     /locus_tag="VIX62_07200"
     CDS             complement(3253..4950)
                     /locus_tag="VIX62_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860054.1"
                     /translation="MTRYLCVDPSHTLGGEPAAIDLTAQVRLEREFLPRESAYLADSG
                     DVIRRVNDLGEVDGALRAAAVRRILTGTTTPLDAPVVLVAGDRTERVPGLYEMDELRR
                     SERSVASLLDRYAQTSLAREGSEDLVVLRDQQPPLRPDGSTVQPGQGPTVQSAPISPM
                     VETVSIPEVRSDEAIVLPQGVYPVLELATRLAEADVETDSLVDVIHATPSYGSLATDE
                     SHRPWRVIVECPVATGEPLTRHQTIFTGTGETEPVEVLGTPAVGNDVVLDTAASKEAV
                     MPFPARRAERGLQIALLAAALIAAGVLIITWISGGLALAVRETPVWLGLSVTLGVAAM
                     AVGLVALASRSDAEGNTNDTFVLRRHYASRIDMLWYATLATAVLFSLSVAAGVIPPIL
                     ASETPVPSAAITFDAGRALVTATVQVQTQGLATDQPVTVQMRQYSSETSNGILIGLVT
                     STGDPSGDTLIGETVSLDAGARYMSVQVIMGNDPVTTCTPLEAGGPGCTVVSVPPLGA
                     GIVRLVPATSALDVIAATEPTTSPVVPTPTSGIPTPTASVPTSAVPTTTLSPTASPV"
     regulatory      5130..5275
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5393..6391
                     /locus_tag="VIX62_07205"
     CDS             5393..6391
                     /locus_tag="VIX62_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860055.1"
                     /translation="MAPNARRIVAACLSLAVVMLPASASAALTDQQRAERGTGFIAGM
                     QRPNGSIPAFSPIGSTADAVLAFVATGSGRPAMREALGYLRRQVAAGNVTGVGLQAKV
                     VMAASAVGRDPRTFGGTNLLQAIRSQIGNDGHIDDAAVFDQALGVLAIEAAGVKPATS
                     VTGWLLDAQCPDGGWSYDAPYAPGTDDNHCDDGSGTDFFTSDSNSTSYVVQALAAADR
                     TSWGSDPFAFFETVRDPGHDGWSYSTAFIATDANSTGLVLQAYIAGGEPIPTGGLKSL
                     RTLQYPACGAWAYTWDGDVKGDPDVGATIGAIPGLLRTPFPLSGPVAGPAPATGAC"
     gene            6395..>6936
                     /locus_tag="VIX62_07210"
     CDS             6395..>6936
                     /locus_tag="VIX62_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860056.1"
                     /translation="MRRLRTLTFGLILALPALAPAVAGSAPACAAEASGASHAALVVN
                     TGSQTTTYCVALDASSVTGIHLVQLAGSQFGLQYQLGFGGQAVCQLAGVGASGGDCFG
                     SYPDFWGYWHGNGSGGWSWAGSGAGSASIGDGDMEGWVWGSGDSGTTHAAPPALGFDD
                     VCKDSTPSPTPPPTPGGNGDG"
BASE COUNT         1025 a         2412 c         2468 g         1031 t
ORIGIN      
        1 tggcccgacc ctagcgcagg acgggaccga cccgagacgg tagcgtccac ccatgaacga
       61 cgacgatcga tacgaacgcg gccgagcgcg gttcctggag gtccatgatg agaaggcgct
      121 ggccgccgtc gaggggcgac gggtagaggt agacacaccc gatcacgtcg cgatccgccg
      181 gatcgagcac cgtgtacgtg aatcccacac ggtcctggaa atccttcgcg tgttggtcga
      241 ggtcggacag gttctcttcc aggctcatcg gccgcggcca ggggtcgggg tcgtcgtcga
      301 agtcgtcgga gaacccgggg ctcgcgtgga tgtgttcgat gctcgacatc cacgcggcgt
      361 gatcggcttc gttgtgctgc ggcccgaggg gctccaggac gaatgcctcg gcgaccaggc
      421 cgtccggcgg cgtgaaccca tcgggaacga agtccgtcga catggcccga ccctagcgca
      481 ggacgggacc gacccgagac ggtagcgtcc acccatgagc gacgacgatc ggtacgaacg
      541 cggccgcgcg cggttcctgg aggtccacga cgagaaggcg ctggccgcgg tcgaggggct
      601 gggcgacctg ggccgcatga tcgtcgaggt cgtctacggc gacgtctacg gccgcagcga
      661 actctcgacg cgcgaccggg aactcgtgac ggtggcggcg ctggtgtcgc tcggccggtc
      721 cgcccagctc cctcagcacc tgcgggcctc gctcaaagca ggcctcacgc cgcaagaact
      781 cggcgaggtc atcatgcaga cggcggtgat cgcggggttc ccaccggcga tgaacgcgat
      841 gtcgacgctg cggacgatcg tggccgaacg cgaccggtga ccacggatca gccttcggag
      901 ttcaggcacc cgcccatgaa gtaggtccat ccatcgggtc cctccatcgc ttcgaacgcg
      961 cccacgacgg tgtcgccacg catcacgcgg aacgccatcg ggttcggcgg gtcctgaccc
     1021 gggatcgacg tgcggtagcc ggcgcgttcg atgaggtcac cctcgagaag gccgtcgtcc
     1081 gcgaggacct cgcggatggc cgtgtcctga tcctggacga aactggtggg aagttctccg
     1141 ttgagctgct ggccggtctc gtcgaccggg cactcgggct ggtaggacac gtagcgggat
     1201 gccgggtcgg tcacgacgaa cccggcgccg gagctcgggg ccggtgcact cgagccctcg
     1261 ggagcgcacg tggcgtgcca cgagcccgga ggcagcggga gctcgaacgt tccaccgttc
     1321 tcacgcaact gcatggggta gaccaatccc gcatccgact ggaagaacac cttctggccc
     1381 tcggtcccct cgatcgccac gtggaccccg tcggccttgg cgttgacggc gggaacctcg
     1441 atgccggcgc cgcgctcgat acaacgcacc cggaggagtc cgacggcgtc atcctcgtcg
     1501 atcaccggca cgggcgaagg agcgctcgcc gagggtcgat cgagtcggcc catcgcgccc
     1561 agcagcccgg caccggccgc gatcacgaac accagcatcg cgagcgcgag cgtctgggcc
     1621 acgctgccgc gttcggaagc acaccggatc cgacgtcgaa gagccatgtg tcccagggca
     1681 tcggcacatg cgcggccttc ccttaccccg ttcggcggat cgttgccctc caggcccgga
     1741 cgtcgccctc gtcccggttg gtctcgaaca ccgccgggac gatctcggtc accgaccatc
     1801 cgtcgtcgaa cgtggcgcgg agctcggcct cggtgactcg ccggggaccc catttcagcg
     1861 gctcgcgctc gctgaaacag agcaggtgca acgtgccccc ggggttcacc acgccggcca
     1921 ggctcgccgc gtacaccggc cgttcgtgat cgtcgaacac gtggaacagg ccgcagtcga
     1981 gcgccgagtc gaacgtgcgg ccgagccctt cgagcgcgaa cacgtccgcc accaggaact
     2041 cggcgcgctc caagccgcgc tcgcgtgctt tgtcgatcgc cttgccgatc gcccgcggcg
     2101 aggcgtcgat gcccaacacc gcctcgccct gccgggctat cgcgagcgcg ttctcgcccg
     2161 tgccgcaccc ggcatcgagt accggcccct cgaaccggcc cgcagcgatc agcgcctcga
     2221 cctcccgctg gggccggccg atgtcccagg gcggcacgcc caggtaggaa tcgtcgaagc
     2281 cgcggtcggc gggataggac accatggagc cgttcaccct agtccaccca tcacaggcgg
     2341 gttgcggcgc tcgcggcgcg acgacctaag ctgtcgtggt gacgctcttc cgactccgcg
     2401 acgatgtccc caccctggtg gcgccggtgc tcgtgggcgc cttcgacggc tggatcgacg
     2461 cgggcggcgc ggcatcggct gcggcggaac gactggcgga aggcgccgag acgatcgcca
     2521 cgatcgatcc cgacgcggtg ttcgactacc gttcccggcg tcccgtactc gacgtggtgg
     2581 acggcacgct cacccatctg gattggccgg agatctccgt gggacgccgc acgatcgacg
     2641 gacgcgacgt gctggtgctg cggggacccg agccggactt ccgctggcgc gaactcgcgg
     2701 catcggcatc cgagatcgcg gtgcggctcg gcgccctgca gtggatcagc ctgggcgcga
     2761 tccccgccgc ggtcccgcac acgcgcgcgg tgtcggtgtt cggcacggcc tccgccgatg
     2821 gcctcctggc cgacgacgtc gaacagggcc ccgccggttt gctccgggtc ccggccgccg
     2881 cattgtccgt ggtggagatg tcgatgcagt cctcgggcct gcccaccgtg gggttctacg
     2941 cgcaggtccc ccattacgtg ggcggaccgt tcgcgccggg cgccatcgcg ttgctggccc
     3001 agctcgatcg tcacctgggc atcgaggtgc cactcggcga actggccgag accgcgatcg
     3061 accagcggac acgtctggac gcggccgttg acgccgacga ggacgcgcgg tcctacctgg
     3121 accggttgga gtcgatgacg ggcagcgagg gcgtgccgac cggcgacgaa ctcgccaccg
     3181 agatcgagcg cttcctccaa ggcgaagccg gcggcgagga accgccgcgc ctcacgtgat
     3241 ccgagatcac cgctagaccg gcgaggcggt cgggctcagc gtcgtcgtgg gcacggctga
     3301 ggtcggcacg gaggcggtcg gggtcgggat gcccgaggtg ggcgtcggca cgaccgggct
     3361 ggtcgtgggt tccgtcgccg cgatcacgtc gagcgccgag gtggccggca ccaaccgcac
     3421 gatgcccgcg ccgagcggcg gcaccgacac cacggtgcag cctgggccgc cggcttcgag
     3481 cggtgtgcac gtcgtcaccg gatcgttgcc catgatgacc tgcacgctca tgtagcgagc
     3541 cccggcatcg agcgagaccg tctccccgat cagcgtgtcc ccggagggat cgccggtgga
     3601 ggtgacgaga ccgatgagga tgccgttgct cgtctcggag ctgtactgac gcatctggac
     3661 ggtgaccggt tggtcggtgg ccagaccctg ggtctggacc tgcaccgtgg ccgtcaccag
     3721 tgcgcggccg gcgtcgaacg tgatcgccgc cgacggcacc ggcgtctcgg acgccaggat
     3781 cggcgggatc acgccggcgg cgaccgagag ggagaacagc acggcggtgg ccagcgtggc
     3841 gtaccacagc atgtcgatgc ggctcgcgta gtggcggcgc aacacgaacg tgtcgttggt
     3901 gttgccctcg gcgtccgacc gcgacgccag cgccaccaac cccaccgcca tcgcggcgac
     3961 gcccaaggtc accgacaggc cgagccacac gggggtctcc cgcaccgcga gcgcgagtcc
     4021 gccggagatc caggtgatga tgagcacgcc ggccgcgatc aacgcggcgg ccagcaatgc
     4081 gatctgcaga ccgcgttcgg cccgccgggc cgggaacggc atcaccgctt ccttcgaggc
     4141 ggcggtgtcc aggaccacgt cgttgccgac ggcgggcgtg ccgagcacct cgacgggttc
     4201 ggtctcgccg gtgcctgtga agatcgtctg gtgccgggtc aacggttcgc cggtggcgac
     4261 ggggcactcc acgatcacgc gccagggacg gtgcgactcg tcggtggcga gcgagccgta
     4321 cgagggcgtg gcatggatca cgtcgacgag cgaatcggtc tcgacgtcgg cttcggcgag
     4381 ccgcgtcgcg agttcgagca cggggtagac gccttgcggc aacacgatcg cctcgtccga
     4441 ccggacctcc gggatcgaca ccgtctccac catggggctg atgggtgctg actgcacggt
     4501 ggggccttga ccgggctgca ccgtggatcc gtccggtcgc aagggcggct gctgatcgcg
     4561 caacaccacg agatcctctg agccttctcg ggccaggctc gtctgggcgt atcgatcgag
     4621 cagggacgcc accgaccgct cgctgcgacg gagttcgtcc atctcgtaga ggccgggcac
     4681 gcgttcggtc cggtcgcccg ccaccagcac cacgggcgcg tcgagcgggg tggtggtgcc
     4741 ggtgaggatg cggcgcacgg cggccgcgcg cagggcgccg tcgacctcgc cgaggtcgtt
     4801 cacccggcgg atcacgtcgc cggaatcggc gagataggcg gattcacgcg gcaggaactc
     4861 gcgctcgagg cggacctggg cggtcaggtc gatggcagcg ggttcaccgc ccagcgtgtg
     4921 gctcgggtcg acgcagaggt atcgggtcat ctcggagaag ctcctttcgc ggcgatcgac
     4981 ccgtgttccg ggccgggctc gcgtgtgcgt tctgcacgaa cctatacccg aggctctgtc
     5041 cagcgatacc ccgattccct aggtcgaaag tcgggacttg ggtccggatg ccgggccgga
     5101 accgtgaacc catgcacgag tggcgggcct ccgcgcgggt ctgctagcgt gcggaacaca
     5161 ccgcgtcgcg gaggcgaagc cggtcagatt ccggcgctgt cccgcaactg taggcacacc
     5221 ggcgctcacc agcgtcacgt gtgcgagcca ggccgccctg cgcgacgcat cggctgaacg
     5281 accctcgagg aacggggacg ggccgccggc gccgatccgg gcgccgacgt cgatccctca
     5341 tcgggtggtc cgttccgtga gggcgcgatg gacgcccgga aaggagccac tcgtggcccc
     5401 gaacgctcgg cgcatcgtcg ccgcctgctt gtccttggct gtcgtgatgc ttcccgcgtc
     5461 ggcgtcggca gcgctcaccg atcaacagcg agccgaacgc ggcaccgggt tcatcgccgg
     5521 catgcagcga ccgaacggct cgatcccggc gttctcaccg atcgggtcga ccgccgacgc
     5581 ggtcctggcc ttcgtggcca ccgggtccgg ccgccctgcg atgcgcgagg cgctcggcta
     5641 cctccgccgc caggtggcgg cgggcaacgt caccggcgtg ggtctgcagg ccaaggtcgt
     5701 catggcggcg tcggccgtcg gccgtgaccc ccgcaccttc ggcggcacga acctgctgca
     5761 ggcgatccgt tcgcagatcg gcaacgacgg gcacatcgat gacgcggcgg tgttcgatca
     5821 ggcgctcggc gtcctcgcga tcgaggccgc cggggtcaag cccgccacgt ccgtcaccgg
     5881 ctggctgctg gacgcacagt gccccgacgg cgggtggtcc tacgatgcgc cctacgcgcc
     5941 cggcacggac gacaaccact gcgacgacgg ctcgggcacc gatttcttca cctcggattc
     6001 gaactccacc tcctacgtcg tgcaggcgct ggctgccgct gaccgcacat cgtggggatc
     6061 ggaccccttc gcgttcttcg agacggtgcg cgatccgggc cacgacggct ggtcctactc
     6121 gaccgcgttc atcgccaccg acgcgaactc gaccggcctg gtgctccagg cctacatcgc
     6181 cggcggcgaa ccgatcccga ccggtggcct gaagtcgttg cgcacgttgc agtacccggc
     6241 atgcggcgcg tgggcctaca cgtgggacgg cgacgtgaag ggcgatcccg acgtgggagc
     6301 cacgatcggt gcgatccccg ggctgttgcg cacgccgttc ccgctgagcg ggcccgtcgc
     6361 ggggccggcg ccggcgacgg gtgcctgctg agccatgcgt cgcctgcgga cgctcacgtt
     6421 cggtctgatc ctcgccctcc cggccttggc gccggcggtg gcgggatcgg ctccggcgtg
     6481 cgccgctgaa gcgtccggcg cctcgcatgc ggccctcgtc gtgaacacgg gatcgcagac
     6541 cacgacgtat tgcgtggccc tcgatgcatc gagcgtgacc ggcatccacc tggtgcagct
     6601 cgccggctcg cagttcggcc tgcagtacca gctcgggttc ggtggacagg cggtgtgcca
     6661 gctcgccggg gtgggcgcga gcggcggcga ctgcttcggc tcctatccgg atttctgggg
     6721 gtactggcac gggaacggct ccggcggatg gagctgggcc ggttcggggg cgggttcggc
     6781 ctcgatcggt gacggcgaca tggaagggtg ggtgtggggt tcgggcgact cgggcaccac
     6841 gcacgccgct ccgccagcgc tcgggttcga cgacgtctgc aaggactcga ccccgtcacc
     6901 gacgcctcca ccaacgcccg gcggaaatgg cgacgg
//
LOCUS       DATDBZ010000296         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2553619, whole genome shotgun sequence.
ACCESSION   DATDBZ010000296 DATDBZ010000000
VERSION     DATDBZ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553619"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VIX62_07215"
     CDS             complement(<1..851)
                     /locus_tag="VIX62_07215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY5860057.1"
                     /translation="MRAVVCHSFGPLDQLTVEEQEAPAACGPGAVRIEVAAAGVNFVD
                     ALLAQGLYQLKPPLPFVPGSEVAGTVVELGEGVDSVTLGDRVLATGGLAGGFAEQMIA
                     RAAQVMAAPVTLSDGQVATFFQSYLTAWFALRERAHAEVGQTILVLGAGSGIGLAAVD
                     VARALGLTVIAGASSAEKRDLARTLGAAEVIDTSTENVKDRARELSGGGLDLVYDPVG
                     GDLAEQGLRALADDGQLLVLGFASGTIPRLPANQVLLRNRRVTGVDWGGWIAKHQERN
                     AELIDEV"
     gene            complement(896..2134)
                     /locus_tag="VIX62_07220"
     CDS             complement(896..2134)
                     /locus_tag="VIX62_07220"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEY5860058.1"
                     /translation="MTEAERIHSLRRMKAVATGLLLAATLVFIITRGAQDGRPWVGYV
                     RAFAEAAMIGALADWFAVTALFRHPLGIPIPHTAIIPNRKDQIGRSLGEFVQGEFLNH
                     DVIVERLEGLNVAHRLGTWLTDPVNAHRAGDGVADALRGTLEVLDDHDVQGALENLVE
                     RRVRATAVAPLIGRAVDLAIEGGHHQRLLDSVLIAVRSFIDDNRSVLREKLDRESPWW
                     VPEPIDDRIFAKIFNGVDSFLAEVAGHPEHEVRHALDARIIAFAQRLRDDPTLVAKGE
                     ELKEELLAHPDVRAWLATMWSEIKRTLITTSSDPTSELRIRLDNGLVQFGEKLCTDPE
                     LKEKIDTWVERAVAHIVDNYRNEVSDLIASTVERWDADATSRKMELQVGRDLQFIRIN
                     GTLVGGLAGLAIHTIGEVFF"
BASE COUNT          358 a          787 c          778 g          346 t
ORIGIN      
        1 aacacctcgt cgatcaactc ggcgttgcgc tcctggtgct tcgcgatcca gccgccccag
       61 tcgacaccgg tcacgcgtcg gttgcggagc aggacctgat tggcggggag tcgcgggatc
      121 gtccccgacg cgaagccgag caccagtagc tgcccgtcgt cggcgagcgc ccggagtccc
      181 tgctcggcga ggtcgccgcc gaccgggtcg tacacgaggt cgagaccgcc cccagacagc
      241 tcccgggccc gatccttcac gttctccgtg gaggtgtcga tcacctcggc ggccccgagc
      301 gtgcgggcga gatcccgctt ctcggcggac gatgcgccgg cgatgacggt gaggccgagg
      361 gcgcgggcga catcgactgc ggcgagcccg atgccgctcc ccgcgccgag cacgaggatc
      421 gtctggccta cctcggcgtg ggcgcgctcg cgcagtgcga accacgcggt caagtagctc
      481 tggaagaacg tggcgacctg gccgtcgctc agcgtgaccg gtgccgccat gacctgcgcc
      541 gcccgggcga tcatctgctc ggcgaacccg ccggcgagcc cgccggtggc gagcacccga
      601 tcgccgaggg tcacggagtc gacaccctca ccgagctcga cgaccgtgcc cgccacctca
      661 ctgccaggga cgaacggcag cgggggcttg agctggtaga gcccctgggc gagcagggcg
      721 tccacgaagt tgacgccggc ggcggccacc tcgatccgca ctgcgccggg gccgcacgcc
      781 gccggcgcct cctgctcttc gaccgtcagc tggtcgagcg gtccgaacga gtggcagacg
      841 acagcacgca cggcgccgac cgtagcgatc ggcgcgggct tgtcagaaga tcacctcaga
      901 agaagacctc accgatggtg tggatggcga gaccggcgag accgccgacg agtgtgccgt
      961 tgatccggat gaactggaga tcccgcccga cctgcagctc catcttgcgg ctcgtcgcgt
     1021 cggcgtccca gcgctcgacg gtggaggcga tcaggtcgga cacctcgttg cggtagttgt
     1081 cgacgatgtg ggcgaccgcc cgttccaccc aggtgtcgat cttctccttc agctcggggt
     1141 cggtgcacag cttctcgccg aactgcacga ggccgttgtc gagccggatg cggagctcgc
     1201 tcgtcgggtc gctcgacgtg gtgatcagcg tgcgcttgat ctccgaccac atggtcgcca
     1261 gccacgccct gacgtcgggg tgggcgagca gctcctcctt cagctcctcg cccttggcga
     1321 cgagcgtggg atcgtcccgc agccgctgcg cgaacgcgat gatcctggca tcgagcgcgt
     1381 gacggacctc gtgctccggg tgacccgcga cctcggcgag gaacgagtcc acgccgttga
     1441 agatcttcgc gaagatccgg tcgtcgatcg gctcgggcac ccaccacggt gattcgcggt
     1501 cgagcttctc ccgcagcaca ctgcggttgt cgtcgatgaa cgagcgcacg gcgatcagca
     1561 cggagtcgag caggcgttgg tggtgcccgc cctcgatggc gaggtccacc gctcggccga
     1621 tgaggggtgc aacggcggtg gctcggaccc gtcgttcgac gaggttctcg agcgcgccct
     1681 gcacgtcgtg gtcgtcgagg acctccaagg tgccccgcag cgcgtcggcg acaccatctc
     1741 cggcgcgatg ggcgttcaca ggatcggtca accacgtccc gaggcgatgg gccacgttca
     1801 ggccctcgag ccgttcgacg atgacgtcgt ggttgaggaa ctcgccctgc acgaactcgc
     1861 caaggctgcg gccgatctgg tccttccggt tcgggatgat cgccgtgtgc gggatcggta
     1921 tcccgagcgg atggcggaac agcgcggtga cggcgaacca gtcggccagc gcaccgatca
     1981 tcgccgcttc cgcgaacgcc cgcacgtagc ccacccacgg ccggccgtcc tgcgcgcccc
     2041 gcgtgatgat gaacacaagg gtcgccgcca gcaacaaacc ggtggccacg gctttcatgc
     2101 gccgcaacga atggatccgt tcggcctccg tcacgactcg ccagggtaac ggtgacccgt
     2161 ggcaggatcc gcgatgccga caggggctac ggcgaccgaa ggggatcacc acatgactgc
     2221 accgagcgag acagcactga gcgagatcac gatggggctg cgctttccc
//
LOCUS       DATDBZ010000297         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_309970, whole genome shotgun sequence.
ACCESSION   DATDBZ010000297 DATDBZ010000000
VERSION     DATDBZ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309970"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VIX62_07225"
     CDS             complement(<1..155)
                     /locus_tag="VIX62_07225"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY5860059.1"
                     /translation="MLLELRAPTAEDTREVGEALSASLRARDAVVLTGELGAGKTTFV
                     QGVARGLG"
     gene            complement(156..893)
                     /locus_tag="VIX62_07230"
     CDS             complement(156..893)
                     /locus_tag="VIX62_07230"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY5860060.1"
                     /translation="MTRTLEQAAAEAASCTKCRLAEGRTQVVYGVGAPDADLLFIGEG
                     PGFHEDKQGEPFVGAAGQLLTKMLQGIGLERSEVYIANIVKCRPPGNRDPQPDEIEAC
                     TPWLVEQISLIQPRVVVTLGNFATKFVLNTSTGITRLRGQVHDWHGRTVIPTFHPAAI
                     LHGGGERSRQFQLLKDDFALVRSTLDDPGTREAPETPEPAIAASEPASPEPATAAALE
                     ATASAPGLSDRIVVPETDPADEQLELF"
     gene            complement(890..1945)
                     /locus_tag="VIX62_07235"
     CDS             complement(890..1945)
                     /locus_tag="VIX62_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5860061.1"
                     /translation="MTRKRAAVIGAGLAAGAVAAGAVGRTIVRRRNEHDLEAHLWDLP
                     PDDLGMIESFDGSKIAARASGDPEAPVLLFVHGFSLDMTTWHEQWIDLSADFRCVVMD
                     QRGHGRSGPPAHGNLSLRALGRDIAAVLDVVAPDRPAVVVGHSMGGMAAIALAEQRPE
                     LFGPRIAGLILVGCSSTDLVRAALGSVSSLLRPRLGLSVTAERVDRLRKAVFASPGDL
                     RGTAIRLTQFGPDAPAHLVEHVGNLSERASSQVWTDGLSGLMEMDLRYALPRVTVPTL
                     VVVGQHDRITPPAASVALAGELPDATLVVLEGAGHVAMMERPVELDREIRAFSRKLLM
                     PASTKRRPKDRSKEQRA"
     gene            complement(1942..>2530)
                     /gene="alr"
                     /locus_tag="VIX62_07240"
     CDS             complement(1942..>2530)
                     /gene="alr"
                     /locus_tag="VIX62_07240"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY5860062.1"
                     /translation="LDRFQGAVMAARAAGHEPGMLHAANTAGSILYPEAQLDMVRTGI
                     GIYGVEPAPGVGAELELRPAMAWRSTVTMAKRLTVGERVSYGHRYELERDAWVATVPV
                     GYADGYPRALASRADVLIGGRRCRVAGNVTMDQLMADCGEHAPQAGDPVVLLGAQGDE
                     VVTANELGPLAGSIGYEIVTRVGARVPRRYLGARP"
BASE COUNT          378 a          924 c          861 g          367 t
ORIGIN      
        1 ccgagtccgc gcgccacgcc ttgcacgaag gtggtcttgc cggcgcccag ctcacccgtc
       61 agcacgacgg cgtcgcgagc gcgcaaggac gccgacaacg cctcccccac ctcgcgggtg
      121 tcctcggccg tcggggcccg aagctccaac agcactcaga acagctccaa ctgctcgtcg
      181 gccggatcgg tctcgggcac cacgatgcga tcgctgagcc cgggcgccga cgccgtcgcc
      241 tcgagggcag cagcagtagc gggttcaggg ctcgcgggtt cgctggcggc gatcgcgggc
      301 tcgggggtct caggcgcctc gcgcgtgccc gggtcgtcga gcgtcgatcg gaccagggcg
      361 aagtcgtcct tcagcagctg gaactggcgc gaccgctcac cgccgccatg caggatcgcc
      421 gccggatgga acgtggggat caccgtgcgg ccgtgccagt cgtgcacctg accccggagc
      481 ctcgtgatcc cggtcgaggt gttgagcacg aacttggtgg cgaagttgcc cagggtcacg
      541 accacgcgcg gttggatcaa cgagatctgc tcgacgagcc acggcgtgca ggcctcgatc
      601 tcgtcgggtt gcggatcgcg gttgccgggc ggccggcact tcacgatgtt cgcgatgtag
      661 acctcggaac gctccagccc gatgccctgg agcatcttcg tgagcaactg cccggcggcg
      721 cccacgaagg gctccccttg cttgtcctcg tggaagcccg gcccctcccc gatgaacagc
      781 aggtcggcgt cgggagcgcc gacgccgtag accacctgcg tgcggccctc ggccagacgg
      841 cacttcgtgc acgacgccgc ctcggcggcg gcttgttcca gggtccgggt catgcgcgct
      901 gctccttgga tcgatccttg ggccgtcgtt tcgtgctcgc cggcatcagg agcttgcggc
      961 tgaaggcacg gatctcgcga tccaactcca ccggacgctc catcatggcg acgtgtccgg
     1021 cgccctccag gaccacgagc gtggcatcgg ggagttcgcc cgccagcgcc accgaggccg
     1081 ccggcggcgt gatgcggtcg tgctgaccga ccacgacgag tgtggggacc gtgacgcgag
     1141 gaagggcata ccgcagatcc atctccatca gtcccgacag cccatcggtc cacacctgcg
     1201 aggacgcgcg ctccgagagg ttcccgacgt gttccacgag atgagccgga gcgtcggggc
     1261 cgaactgcgt gagcctgatc gcggttcccc gaaggtcgcc cgggctcgcg aacacggcct
     1321 tgcggagacg gtcgacgcgc tcggcggtca cgctgagacc cagccgcgga cggagcaggc
     1381 tcgagaccga cccaagggcc gcgcgaacca ggtcggtcga cgagcagccc accaggatca
     1441 gtccggcgat ccgcggaccg aacagctcgg gtcgttgctc ggcgagcgcg atcgcggcca
     1501 tgccgcccat gctatggccc acgacgaccg caggccggtc gggggcgacc acgtccagca
     1561 ccgcggcgat gtcgcggccc agggctcgca gggacaggtt cccgtgcgca ggcggaccgc
     1621 tccggccgtg tccccgctga tccatcacca cgcatcggaa gtccgccgac agatcgatcc
     1681 attgctcgtg ccaggtggtc atgtccaggc tgaacccgtg gacgaacagc agtaccggcg
     1741 cctccggatc gcccgatgca cgtgccgcga tcttcgaccc gtcgaaggac tcgatcatgc
     1801 ccaggtcgtc gggcggcagg tcccacagat gggcttcgag gtcgtgctcg ttcctgcgtc
     1861 ggacgatcgt gcgtcccacg gcgcccgccg cgaccgcgcc ggcggcgagc ccggcgccga
     1921 tcaccgcggc tcgcttgcgc gtcacggtcg tgcccccagg tagcgtcgcg gcaccctggc
     1981 cccgacacgc gtgacgatct cgtagccgat cgaccccgcg agcggcccga gctcgttcgc
     2041 ggtgacgacc tcatcgccct gggcgccgag cagcacgacg gggtcgcccg cctgaggggc
     2101 gtgttcaccg caatcggcca tcaattgatc catcgtcacg ttccccgcca cccggcaccg
     2161 tcgtcctccg atcaggacat cggcgcggga ggccagcgcg cgaggatagc cgtcggcgta
     2221 gcccaccggc accgtggcga cccacgcgtc acgttccagc tcgtagcgat gcccgtagct
     2281 gacgcgctcg cccaccgtga gccgcttcgc catggtgacc gtggaccgcc aggccatcgc
     2341 ggggcgcagc tcgagctcgg cgcccacccc cggcgccggt tccacaccgt agatcccgat
     2401 cccggtgcgc accatgtcca gctgcgcttc cggatacagg atcgaaccgg cggtgttcgc
     2461 ggcgtggagc atgccgggtt cgtggccggc agcccgggcc gccatgacgg ccccttggaa
     2521 gcgatcgagc
//
LOCUS       DATDBZ010000298         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_719539, whole genome shotgun sequence.
ACCESSION   DATDBZ010000298 DATDBZ010000000
VERSION     DATDBZ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719539"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VIX62_07245"
     CDS             complement(<1..255)
                     /locus_tag="VIX62_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY5860063.1"
                     /translation="MIADIVQTGLAAWWAPVLAFAAGLVSCLSPCVWPLLPGYVSFIT
                     GDRIVSGEAGRARSLTPMLLFIAGFTIVFVLLGAFASTFVQ"
     gene            444..869
                     /locus_tag="VIX62_07250"
     CDS             444..869
                     /locus_tag="VIX62_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860064.1"
                     /translation="MSQVESTTPTTATQTRKARVEAFLRDFEWTWTTAVVFSLGLVFF
                     LLITTSVLPSFFLYVSEQKFGWQGPTDIQAALEELPTLLPGGEPAVYDELLLQVRDGV
                     AMGLSTVPIIIVLVGGAILQNWRRKLRGQSDNRPTGGYR"
     gene            881..1279
                     /locus_tag="VIX62_07255"
     CDS             881..1279
                     /locus_tag="VIX62_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY5860065.1"
                     /translation="MPYTKTEVDIHEDYTVEAVDPAWLAQGVKSPKHLMLDPDNCILC
                     RACEDVCPWNCIYMMSPGIVSGAADHVVGDEVDNSNAVFVVDDNACTRCSVCVDRCPT
                     DTLYYARLPEGSKGGARTMAEVPTSQAEAG"
     gene            1299..2069
                     /locus_tag="VIX62_07260"
     CDS             1299..2069
                     /locus_tag="VIX62_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY5860066.1"
                     /translation="MKLKPPSPGELGDRIRETEVWKSIFRPGSIFRKGYKDTSRDRAL
                     ATMNNVLYHLHPVKVKRHGLKLTYTFCLGGLSFFLFILLTITGIFLMFFYRPTADIGG
                     AQAFADMQDIRTSVFFGDLVRNLHRWGAHLMVFSVTLHMARVFYTGAYKPPREFNWVV
                     GVILLFLTLGLSFTGYLLPWDQLAIWAVTVGTSLAGYSPFIAKQANFLLLGGVVVGPE
                     TLLRWYTLHVIGLPFVLVIFLAVHFWRIRKDGGISGPL"
     gene            2087..>2305
                     /locus_tag="VIX62_07265"
     CDS             2087..>2305
                     /locus_tag="VIX62_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860067.1"
                     /translation="MAKEDTPLDQGVFDSTLEAELAKGTDRRVAEGRARSAAVRAFRA
                     ANPEEAAKHEAAGAGAPAAAAAAGADAPA"
BASE COUNT          387 a          787 c          772 g          359 t
ORIGIN      
        1 ctgcacgaag gtcgaggcga acgcgccgag cagcacgaat acgatcgtga acccggcgat
       61 gaacagcagc atcggcgtga gcgaccgggc gcggcccgcc tcgccgctca cgatgcggtc
      121 gccggtgatg aacgagacgt agccgggcag cagcggccat acgcaggggc tgagacacga
      181 cacgagcccc gcggcgaacg ccagcacggg cgcccaccag gcggcgagtc cggtctgcac
      241 gatgtcggcg atcaacgatg gctccaggct ggttgtcttg cgtggaccga gggtccgcag
      301 cgatagtaga cggcgcgctt gtggatcgat ccacgagccc cgcgagagtg gaagacggtc
      361 gtcgggcgcg tgcggcacat gtctataatc cccccgcctc aggcagactc gcccgacgag
      421 acacgcgagg aacacaggag cgtatgagcc aggtcgagag cacgacgccc accacggcca
      481 cgcagacccg aaaggcgcgc gtcgaggcgt tcctcaggga cttcgagtgg acgtggacca
      541 ctgcggtcgt cttctcgctg gggttggtgt tcttcctgct gatcacgacc tcggtgctgc
      601 cgtcgttctt cctctacgtg agcgagcaga agttcggctg gcagggcccg accgacatcc
      661 aggcggccct cgaagagctg cccacgctgc tgccgggcgg cgagccggcc gtctacgacg
      721 aactgctgct gcaggtgcga gacggtgtcg cgatgggcct cagcacggtg ccgatcatca
      781 tcgtgctcgt cggcggcgcc atcttgcaga actggcgccg caagctgcgc gggcagtccg
      841 acaaccgtcc gaccggcgga taccggtagg gtcgggcgcg atgccgtaca cgaagaccga
      901 ggtcgacatc cacgaggact acaccgtgga agcggtcgac cccgcgtggc tcgcccaggg
      961 cgtgaagtcg ccgaagcacc tgatgctcga ccccgacaac tgcatcctgt gccgcgcgtg
     1021 cgaggacgtc tgcccgtgga actgcatcta catgatgtcg ccgggcatcg tctcgggcgc
     1081 ggccgatcac gtcgtcggcg acgaggtcga caactcgaac gccgtgttcg tcgtcgacga
     1141 caacgcctgc acccgttgct cggtgtgcgt cgaccgctgc cccaccgaca ccctgtacta
     1201 tgcgcgactg cccgagggtt ccaagggcgg cgcacgcacg atggccgagg tgcccacgtc
     1261 gcaggccgag gccggatgat cgagcagagg gagcagacgt gaagctgaag cctccgtcgc
     1321 ccggcgaact gggtgaccgg atcagggaga ccgaggtctg gaagtcgatc ttccggccgg
     1381 ggtcgatctt ccgcaagggg tacaaggaca cgtcccgaga ccgggccttg gccacgatga
     1441 acaacgtgct ctaccacctg caccccgtga aggtgaaacg gcacgggctg aagctgacct
     1501 acacgttctg cctgggcggg ctgtcgttct tcctgttcat cctgctcacg atcaccggca
     1561 tcttcctgat gttcttctac cggcccacgg ccgacatcgg cggcgcccag gcgttcgccg
     1621 acatgcagga catccgcacc tcggtcttct tcggcgacct cgtgcgcaac ctgcaccggt
     1681 ggggcgccca cctcatggtc ttcagcgtca ccctgcacat ggcgcgcgtg ttctacaccg
     1741 gggcgtacaa gcccccgcgc gagttcaact gggtcgtcgg cgtgatcctg ctgttcctca
     1801 cgctcggcct gtcgttcacc ggctacctgc tgccctggga ccagctcgcg atctgggcgg
     1861 tcaccgtggg cacgtcgctg gccggctact cgccgttcat cgcgaaacag gcgaacttcc
     1921 tgctgctcgg cggcgtggtg gtcgggcccg agaccctgct ccgctggtac acgttgcacg
     1981 tgatcggact gccgttcgtg ctggtcatct tcctcgcggt gcacttctgg cgcatccgca
     2041 aggacggcgg catctcggga ccgctctaag ggatccaagg ggagacatgg ccaaggaaga
     2101 caccccgctc gaccaaggag tcttcgactc cacgctggag gcggagctcg ccaagggcac
     2161 cgaccgccgt gtggccgagg gacgcgctcg gtcggcagcc gtgcgcgcct tccgcgccgc
     2221 caaccccgag gaagccgcga agcacgaggc cgccggcgcg ggcgctccgg ccgccgcagc
     2281 cgctgcgggc gccgacgctc cggcc
//
LOCUS       DATDBZ010000299         7725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1324893, whole genome shotgun sequence.
ACCESSION   DATDBZ010000299 DATDBZ010000000
VERSION     DATDBZ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324893"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VIX62_07270"
     CDS             complement(<1..540)
                     /locus_tag="VIX62_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5860068.1"
                     /translation="MRYRVEQLAAISDISVDTVRYYQSRGLLPQPEREGRVAWYGPEH
                     VERIAQIRSLQRKGLTLAAIRRVISGELGVADADLAAAVAAARTESGEPATELLDLDA
                     FSARSGVPASLIQAVEREGIRLGRTVDGEERYTAADIDLVRTALRLLEFGLPLADLLA
                     LARDADQAMRGLAERAVELF"
     gene            633..1889
                     /locus_tag="VIX62_07275"
     CDS             633..1889
                     /locus_tag="VIX62_07275"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY5860069.1"
                     /translation="MKRVARPVVSLGVVLACVIGTMALGTALKSPCAGGSWSDVRQYR
                     FLCYSDIVPLLATEQLFERGGRLPFLDECEVREGQNCDEYPVVTMYFIRAAGWLSGDD
                     YTRFYLVNALMLLGCAALVAVCLYVMNGSRALYFALAPTLVIYGTMNWDLLAVAFATV
                     GLLYFFRRREVAAGAALGIGAAAKLYPGMLALPLIVQRLRDRRPDRAIGLAWSTAGAW
                     LLVNLPFAIAAPSAWWTFFRFNSERAADFDSLWYIACRHLEFCPTTRTVNLLSLASFL
                     GLFAIVWVVKARREPSFPRWTLGFPMLVLFLVTNKVYSPQFGLWLLPWFALALPRIRP
                     WLAFQVADVLVFVTRFWFFGWLAGVFGVSQGTFEAMVLVRAIVLAGCVVAWVRWHAEP
                     LEIEARAAPEPVPVADAPGSPEAQPA"
     gene            1886..3055
                     /locus_tag="VIX62_07280"
     CDS             1886..3055
                     /locus_tag="VIX62_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEY5860070.1"
                     /translation="MSEVTGETNAPRLRDGIRDCVLVFLGVRVGLSVLSLISVALIEP
                     REGVPAVPGWVIGPVTYGWQAIVTATERQDAAWFLRIATGGYAAGDGSAAFFPLYPMA
                     IKVLAWLPGVGPLGAALFISNACFLGALVVLHGLTRLEGMSTAVARTSVLLIAIFPTA
                     FFFLAPYTESPFLLLSVSAFWFARRDRWAMAALVGALAALTRSIGLVLVFGLAVEAIQ
                     RTREDGRPLVPRLAASAAVAAGPALYFLYWGVVHQDAMAPFEAQESWQRVFTFPLATL
                     VQAGRFAWQYGTYWLIDALVVSVVLVAVLAGIRWLRWGYLAYALASLAVPLFEPLADR
                     PLLSMPRFVVVLFPAFWIMARAVERRRLPEPLLVASFAGGYALLGALFINWWHVF"
     gene            3135..3803
                     /locus_tag="VIX62_07285"
     CDS             3135..3803
                     /locus_tag="VIX62_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860071.1"
                     /translation="MRNRTATGEHVTIRVAIADDHSLVRQGLRRYLDMADGIEVVGEA
                     ANGQELLTLIEGTTPEIALVDIRMPEMDGLEAAREIRDRWPEVGVIMLTAYDDRQFVV
                     EAVRSGARGYVLKARDAEHLIQTVRLVAGGNMVIDPQLVVALAEELSTAKERDRRAET
                     LTAREIEVLQLLAFGHTNRDIAERLYISPDTVKTHLEHIFEKLGASDRTAAVAEALRR
                     RLIE"
     gene            3910..6036
                     /locus_tag="VIX62_07290"
     CDS             3910..6036
                     /locus_tag="VIX62_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5860072.1"
                     /translation="MQDAVVSSRPRLFLGGILALAAVVSVGTYLAFPSGSREQAFAAE
                     ALIAGSCFLSGGVILRAVGRGQGWPGSAYLAWALFLLGAASLAFLTMTVRDPGLYVPG
                     PSDLAFALFLIPIVGLARAEYREHFEVRDRREIGVDVFLITASLTALLYVMIRPIEAD
                     VQASLAAGIFAALTAVQIAAFGALLLWRRSTTHLLLFAIFAAMAAATASFGWQWTHGT
                     FDGAAAPIDIPFMLCPLAFAALVVYSPGHRASLEQEPGRWARPVLTSISVVAACAALA
                     VVAALDAARGIEGVQSVLIIGLLGVGVAARILSNQIASTQAHRATAEALAEKATALDE
                     ADAALERVREANETLRESEEHLRLVFDTAVDGIVELDDRGLVIRANGSFSDMVGLSRE
                     AIEGEPWSALAASVSGADAGVAALPAGGQAQIQRGEGQALYLESRVSQVPTNPPRKLV
                     LVRDVSAAKVADQTIRSLFQFLQDRDEDRTRLLRRTNAAIEAERNRVARDLHDGPVQG
                     VSAASLSLEAALLMIKAGETDRGLEVLTKIREELAGEADALRRLMSGLRPPVLEERGL
                     IPALRETLSRFGTDHEVKTEFNGRVASTIPEDLETLAYRVVQEALSNAGKHASAAFVT
                     VSVEADDSQLKIEIEDDGCGFESAQTREFLRQGRVGLASMRERVELASGTFVVRSSPG
                     RGTAIMATLPVDDSPAKREPVLHEAS"
     gene            complement(6033..>7725)
                     /locus_tag="VIX62_07295"
     CDS             complement(6033..>7725)
                     /locus_tag="VIX62_07295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY5860073.1"
                     /translation="DQILAKYLNTVYFGHGAYGAEAAAQTYFGIPASDLDVTQSAVLA
                     AVLHAPSLYDPIKSSYDNKFRRDYTLDQMAKYGYITEAEAASLKAEDCCGIPDSQLEE
                     QARIVSPPGTEYFVDYTRQELFDKYGSAKVYGGGLQVTTTLDMRLQRAAQAAVDANLP
                     DTPENPDASVVTIDNATGEILAMVGGRDWDKSKLNLATMPCEGCGRQAGSAFKPFTLA
                     AALEQDYSFVGAYWQGPSQITITDPTCAGPDGLWAPVNAGDGEAGTFTLYTATEHSVN
                     TVYAQLVSQIGPESVVEMAKRLGIKSKLDAFCSVTLGSVAVNNLEMTNAYSTLAAHGM
                     LHRATPVLEVTRQNGAPIQKHFLPPGKQVLDPNIADEVTYALGGVVTGGTGYAADLRY
                     PYGYYVYGKTGTAQDNVDAWFCGYTQELSTCVWVGYPKEQTPLLYVEGVPAVYGGTIP
                     AAIWHDYMLAAMDIYAPEGGDPYEQPEFTGTLGPSVPVASPTPPPSPTVTPSPSTEPT
                     PTDDPSPTDDPSPTETPSPTVEPKAFLQATIVPPENDRPTLGRHRQLTHRHTPKR"
BASE COUNT         1066 a         2600 c         2707 g         1352 t
ORIGIN      
        1 gaacaactcg acggcacgct cggcgagccc gcgcatcgcc tggtcggcat cgcgggccag
       61 tgccagcagg tcggcgagcg gcagaccgaa ctccaagagg cgcagcgccg tcctgaccag
      121 gtcgatgtcg gcggcggtgt agcgctcctc accgtcgacg gttcggccca gccggatgcc
      181 ctcgcgctcc accgcctgga tcaacgatgc cggcacgccg ctgcgcgccg agaacgcgtc
      241 cagatccagg agttcggtcg cgggttcgcc gctctcggtc cgagcggcgg cgacggcggc
      301 cgcgaggtcg gcatcggcca cgcccagctc gccgctgatc acgcgccgga tcgcagccag
      361 cgtgagtcct ttgcgctgga gcgaccggat ctgagcgatc cgctcgacgt gttccggccc
      421 gtaccaggcc acgcgtcctt cacgttcggg ctgcgggagc aagccgcgcg actggtagta
      481 gcgcacggtg tcgaccgaga tgtcgctgat cgcggccagc tgttccacgc ggtacctcac
      541 gaccagacac tctacgagtg ttcgctcaca cggtcatccg cgacggtcac acaggcttcc
      601 gcgctcgggt ccccgggcta cgatgcgccc ggatgaagcg cgtcgcccgg ccagtggtca
      661 gcctcggtgt ggtcttggcc tgcgtgatcg gcacgatggc cttggggacg gcactgaaat
      721 cgccctgcgc cggcggctcg tggagcgacg tccgccagta ccgcttcttg tgctactccg
      781 atatcgtgcc gctcctggcc accgagcaac tcttcgaacg aggcgggcga ctccccttcc
      841 tcgacgaatg cgaggtccga gagggccaga actgcgacga gtaccccgtg gtcacgatgt
      901 acttcatccg cgccgcgggg tggctctcag gcgatgacta cacccgcttc tacctggtga
      961 acgccctgat gttgctgggc tgcgccgcgc tggtcgcggt ctgcctctac gtgatgaacg
     1021 gctcgcgcgc gctctacttc gccctggcac ccaccctggt catctacggc acgatgaact
     1081 gggacctgct cgccgtggcc ttcgccacgg tggggctcct gtacttcttc cgaagacgcg
     1141 aggtggccgc cggtgccgcg ctcggcatcg gtgccgccgc gaagctctac ccggggatgc
     1201 tggcgctccc gttgatcgtg cagcgcctgc gcgaccggcg accggaccgg gcgatcgggc
     1261 tggcgtggtc caccgccggc gcgtggttgc tggtgaacct gccgttcgcg atcgccgcgc
     1321 cgagtgcgtg gtggacgttc ttccgattca acagcgagcg cgccgcggac ttcgacagcc
     1381 tctggtacat cgcgtgccgc catctggagt tctgcccgac gacgcggacc gtgaacctgc
     1441 tgtccctggc atcgttccta gggttgttcg cgatcgtgtg ggtcgtgaag gcgcgcaggg
     1501 aaccgtcgtt cccgcggtgg acgctgggct tcccgatgct ggtcctgttc ctggtcacca
     1561 acaaggtgta ttcaccgcag ttcggattgt ggctgctgcc ctggttcgcg ctggccttgc
     1621 cgcggatccg cccatggctc gccttccagg tcgccgacgt gctggtgttc gtgacccggt
     1681 tctggttctt cggctggctg gcgggggtgt tcggcgtctc gcagggaacg ttcgaggcga
     1741 tggtgctagt gcgggcgatc gtgttggcgg gctgcgtggt ggcctgggtc cgctggcacg
     1801 ccgaaccact cgagatcgag gcgcgcgccg cgcccgaacc cgtgccggtg gccgacgcgc
     1861 cgggctcgcc ggaggctcag ccggcgtgag cgaggtcacc ggcgagacga acgcaccccg
     1921 gctgcgcgac gggatccgcg actgcgtcct cgtcttcctc ggcgttaggg tcgggctgag
     1981 cgtgctctcg ctgatctcgg tcgcgttgat cgagccgcgg gagggcgtgc ccgccgtgcc
     2041 gggttgggtg atcggtccgg tgacctacgg atggcaggcg atcgtcaccg cgaccgaacg
     2101 ccaagatgcc gcgtggttcc tgcggatcgc gaccggcggc tatgccgcgg gcgacggcag
     2161 cgctgccttc ttcccgctgt atcccatggc gatcaaggtg ctggcctggc tgcccggcgt
     2221 cgggccgctg ggtgcggcgc tgttcatctc caacgcgtgc ttcctcgggg ccctcgtcgt
     2281 gctccacggt ctgacgcggc tggagggcat gtcgaccgcg gtggccagga cctcggtcct
     2341 gctgatcgcg atcttcccca cggcgttctt cttcctagcg ccgtacaccg agtcgccgtt
     2401 cctgctgctg tcggtgtcgg ccttctggtt cgcccggcgc gaccggtggg ccatggcggc
     2461 gctggtgggc gcgctggcag cgttgacccg cagcatcggc ctggtgctgg tgttcgggct
     2521 ggcggtcgag gcgatccagc gaacccgcga agacgggcgc ccgctggtcc cccgactggc
     2581 ggcctcggcc gcggtcgccg cgggtccggc gctctacttc ctctactggg gtgtggtgca
     2641 tcaggacgcg atggcgccgt tcgaggccca ggagtcgtgg cagcgcgtgt tcaccttccc
     2701 gctggcgacc ctggtgcagg cgggcaggtt tgcctggcag tacggcacct attggctgat
     2761 cgacgccttg gtggtcagcg tggtgctcgt cgcggtgctc gcggggatcc gatggctccg
     2821 gtgggggtac ctggcgtacg cgctggcgag cctggccgtg ccgctgttcg aaccgttggc
     2881 cgaccgacca ctattgtcga tgccgcggtt cgtggtggtg ctgttccccg cgttctggat
     2941 catggcgcga gcggtggaac gccgccgact ccccgagccc ctcctggtgg cgagcttcgc
     3001 gggtgggtac gccctgctgg gggcgctctt catcaactgg tggcatgtgt tctgatgcgg
     3061 gccatgacga tcgctggtga cgagcccaca acttctgccc cgatggactc cgcgggcccc
     3121 cgaaccatta ggatgtgcgg aaccgcacgg ccacggggga gcatgtgacg atcagggtag
     3181 ccatcgcgga cgaccattca ctggtacgcc aaggactccg gcgctacctg gacatggccg
     3241 acggcatcga ggtggtcggc gaggccgcca acggccagga actgctgacc ctcatcgaag
     3301 gcaccacccc cgagatcgcg ctcgtcgaca tccgcatgcc ggagatggac ggcctggaag
     3361 ccgcccggga gatccgcgac cgctggcccg aggtgggcgt catcatgctc accgcctacg
     3421 acgaccgcca gttcgtggtc gaagcggtcc gcagcggagc ccgcggctac gtcctgaagg
     3481 cacgcgacgc cgaacacctg atccagaccg tccgcttggt cgccggcggc aacatggtga
     3541 tcgacccgca gctggtggtc gccctggccg aggagctctc caccgccaag gaacgcgacc
     3601 gccgcgccga gaccctcacc gcgcgggaga tcgaggtcct gcagctgctc gcgttcggcc
     3661 acacgaaccg cgacatcgcc gagcgtctgt acatctcgcc cgacacggtg aagacccacc
     3721 tcgagcacat cttcgagaag ctgggcgcct cggatcgcac cgcggccgtc gccgaggcgc
     3781 tgcgacgccg cctcatcgaa tagtccggtc ggcgttcagg gggccacaga gggctaggcc
     3841 gtacgggcca ccatctcctg tgtccgggtg atggagccgg gggctcgaac cctgcatcat
     3901 ggtgacccta tgcaggacgc cgtcgtttcc tcgcgccccc gcttgttctt gggcgggatc
     3961 ctcgcactcg ccgccgtggt ctcggtgggg acctacctcg ccttccccag tggctctcgc
     4021 gagcaggcct tcgccgccga agcgctgatc gccgggtcgt gcttcctctc agggggcgtc
     4081 atcctgcggg cggtcggcag gggtcaggga tggcctggga gcgcctacct ggcctgggca
     4141 ctgttcctgt tgggcgcggc gagcttggcg ttcctgacca tgacggtccg cgatccgggc
     4201 ctctacgtgc ccggtcccag tgatctcgcc ttcgcgctgt tcctgatacc gatcgtcggt
     4261 ctggcgaggg cggagtaccg cgagcacttc gaggtgcgcg accggcgcga gatcggcgtg
     4321 gacgtcttct tgatcacggc ctcgctcacc gccctgctgt acgtgatgat ccggcccatc
     4381 gaggccgacg tgcaagcgtc cctggccgcc gggatcttcg cggcgctgac cgcggtccag
     4441 atcgcggcgt tcggtgcctt gttgctgtgg cgtcgctcca cgacgcacct gctgctgttc
     4501 gcgatcttcg ccgcgatggc cgcggcgacg gcctcgttcg gatggcagtg gacccacggg
     4561 acgttcgacg gtgcggccgc gcccatcgac atcccgttca tgctgtgccc actcgcgttc
     4621 gccgccctgg tcgtctactc gcccggacac agggcgagct tggagcagga gccgggtcga
     4681 tgggctcgac ccgtgctcac gagcatctcg gtggtggcgg cgtgtgccgc cctcgccgtc
     4741 gtcgcggcgc tggacgccgc gcgcgggatc gagggcgtgc agtccgtgtt gatcatcggc
     4801 ctgctgggcg tcggggtggc cgcccgcatc ttgtcgaacc agatcgccag cacccaggcg
     4861 caccgagcca ccgccgaggc gctggccgag aaggcgaccg ccctcgacga ggccgacgcg
     4921 gcgctcgagc gcgtgcgcga agcgaacgaa accctccgcg aatccgaaga acacctccgg
     4981 ctcgtgttcg acaccgccgt ggacggcatc gtggaactcg acgatcgagg cctggtgatc
     5041 cgggcgaacg gttcgttcag cgacatggtt gggctctcgc gcgaggcgat cgaaggcgaa
     5101 ccgtggtcgg cgctggccgc atcggtcagc ggtgccgacg ccggagtcgc cgccctcccc
     5161 gcgggcggac aggcgcagat ccaacggggc gaaggccagg cgctgtacct ggagtcccgg
     5221 gtctcgcagg tgcccacgaa cccgccccgc aagctggtgc tggtgcgcga cgtgtcggcc
     5281 gccaaggtcg ccgaccagac gatccgctcg ctgttccagt tcctccagga ccgcgacgag
     5341 gaccgcaccc gcctgctgcg acgcacgaac gccgcgatcg aagccgagcg caaccgcgtc
     5401 gcgcgcgacc tgcacgacgg gccggtgcaa ggcgtcagcg cggcgtcgct ctcgctcgag
     5461 gccgcgctgc tgatgatcaa ggcgggcgag accgatcgag gcctcgaggt cctcacgaag
     5521 atccgcgaag aactcgccgg agaggccgac gcgctccgcc gactgatgtc gggcctgcgc
     5581 ccgccggtgc tggaggaacg cggactcatc cccgcgttgc gcgagacgtt gtcacggttc
     5641 gggaccgacc acgaggtgaa gaccgagttc aacgggcggg tggcctcgac gatccccgag
     5701 gacctggaga cgctcgccta ccgcgtggtg caggaagccc tgtcgaacgc gggcaagcat
     5761 gcgtcggcgg ccttcgtcac ggtgagcgtg gaagccgacg actcccagct caagatcgag
     5821 atcgaggacg acggctgcgg gttcgagtcg gcacagaccc gggagttcct gcggcagggc
     5881 cgtgtcggcc tggcatcgat gcgcgaacgg gtcgaactcg cgagcggcac cttcgtggtg
     5941 cggtcctcac ccggacgcgg caccgccatc atggcgacgc tgccggtgga cgactcgccg
     6001 gccaagcgcg agcccgtgtt gcacgaagcg tcctagcgct tcggcgtgtg ccggtgggtc
     6061 agctgtcgat gccggccgag cgtcggtcga tcgttctccg gcggaacgat ggtcgcttgc
     6121 aggaacgcct tcggctccac cgtgggactc ggcgtctccg tgggcgaagg atcgtcggtg
     6181 ggtgaaggat catccgtggg tgtcggctcg gtcgagggtg agggcgtcac ggtcgggctc
     6241 ggcggggggg tcgggctcgc aaccggcacc gacggcccga gcgtcccggt gaattcaggc
     6301 tgttcgtatg ggtccccgcc ctcgggcgca tagatgtcca tcgctgccag catgtagtcg
     6361 tgccagatgg ctgcggggat cgtgccaccg tagaccgccg gcacgccttc gacgtagagc
     6421 aacggtgtct gctccttcgg gtagccgacc cacacgcagg tggagagttc ctgggtgtat
     6481 ccgcagaacc aggcgtcgac gttgtcctgc gcggtaccgg tcttcccgta gacgtagtag
     6541 ccatacgggt acctgaggtc ggcggcgtac cccgtgcccc cggtgacgac ccccccaagc
     6601 gcgtaggtca cttcgtcggc gatgttcggg tcgagcacct gcttgcccgg cggaaggaag
     6661 tgcttctgga tcggagcgcc gttctgccgg gtgacctcga gcacgggggt ggcacggtgg
     6721 agcatgccgt gggcggcgag cgtcgagtac gcgttcgtca tctccaggtt gttgaccgca
     6781 acgctgccca gggtcacgga acagaaggca tcgagcttgg acttgatccc cagccgcttc
     6841 gccatctcga cgaccgactc cggtccgatc tgactcacga gttgggcgta gacggtgttc
     6901 accgagtgtt cggtcgcggt gtacaaggtg aaggtccccg cttcaccgtc accggcattc
     6961 accggtgccc agaggccgtc gggaccggcg cacgtcgggt cggtgatcgt gatctgcgag
     7021 ggaccttgcc agtaggcccc cacgaacgag tagtcctgtt ccagcgcggc agccagcgtg
     7081 aacggcttga acgccgagcc cgcttgccgg ccgcagccct cgcacggcat cgtggcgagg
     7141 ttcagcttgg acttgtccca gtcgcgtccg ccaaccatcg ccaagatctc accggtggcg
     7201 ttgtcgatcg tgaccaccga cgcgtcggga ttctcgggcg tatcggggag gttggcgtcg
     7261 acggcggcct gggcggcacg ttggaggcgc atgtccaagg tcgtcgtgac ctggagccca
     7321 ccgccgtaga ccttggcgct cccatacttg tcgaacagct cctgtcgcgt gtagtcgacg
     7381 aagtactcgg tgcctggcgg actcacgatg cgggcctgct cctccagctg ggagtccggg
     7441 atgccgcagc agtcctcagc cttcagcgac gccgcctccg cctcggtgat gtagccgtac
     7501 ttcgccatct gatccaacgt gtagtcgcgc cggaacttgt tgtcgtacga gctcttgatc
     7561 gggtcgtaga ggctgggtgc gtggagcacg gccgccaaga ctgccgactg ggtgacgtcg
     7621 aggtcactgg ccgggatgcc gaaataggtc tgcgccgccg cttctgcacc gtaggcgccg
     7681 tgaccgaagt acaccgtgtt gaggtacttc gccaggatct ggtcc
//
LOCUS       DATDBZ010000300        13270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_507401, whole genome shotgun sequence.
ACCESSION   DATDBZ010000300 DATDBZ010000000
VERSION     DATDBZ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13270
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507401"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VIX62_07300"
     CDS             complement(<1..273)
                     /locus_tag="VIX62_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5860074.1"
                     /translation="MSAPANAETDAELLERLRAGDEDAFMELVDALQPAMLRVARMYV
                     SSQAVAEEVVGDAWLGVLKGLDRFEGRSSLRTWIFRIVTNIAKTRGQ"
     gene            complement(342..1442)
                     /locus_tag="VIX62_07305"
     CDS             complement(342..1442)
                     /locus_tag="VIX62_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY5860075.1"
                     /translation="MTRTTKRLARLTVAVIFVVPTLVGSLVTSSSAAPSKAEVDAAKA
                     KLQDVSEKLEIVIEQYNEAKVRLGGIQDKLDAALADKQEAEAEAAAATEDLEARAVEA
                     YTGSGNQLDVLLGAQDLTEFSDRLEFMGALAQNDADLATQAANARQRADWAAQQYGEA
                     LDQQKAELSKMSANREDIQAMLQEQEALYEQLNKDYQDYLERQEAAAAAAAAAEQNTG
                     STGGTTTSGGGSTDPGGWTEPPPPPPGSGAAAAVAAAKSVIGAPYVWGSANPAVGFDC
                     SGLTSWAWGQAGVYLPHSSASQAYSGAVVPSLSQAAAGDLLFFYSPISHVALYIGGGQ
                     MIHARHPGPGGQVQYGSVSGYGTPVVRIVRPG"
     gene            complement(1667..1740)
                     /locus_tag="VIX62_07310"
     tRNA            complement(1667..1740)
                     /locus_tag="VIX62_07310"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1704..1706),aa:Pro,seq:cgg)
     gene            complement(1782..2663)
                     /locus_tag="VIX62_07315"
     CDS             complement(1782..2663)
                     /locus_tag="VIX62_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY5860076.1"
                     /translation="MVIPVVLAVALVGIGCVLYGILIERRWYRLVRHRLAILPATGPE
                     TLSVLHLSDLHFVRDEPGKSRRFLASLPPADVTVVTGDFLAEPEGVETVVDMLRPTRG
                     RLASWYVLGSNDYFAPRPLNYFAYFQRNRKRRSAVKGRAGDLVTQLNADGWTDLTNVR
                     HDVDLDGLAVELLGLDDAHIRWQDYRDAPRRAPDRFGFAVMHSPDSAPEAASLGYDLI
                     VAGHTHGGQVCLPVVGALVTNCSMPRRLVKGVIGMGGAVLHTSPGMGTSKYAPFRFWC
                     RPEATMLELRRRPEPAP"
     gene            2678..3133
                     /locus_tag="VIX62_07320"
     CDS             2678..3133
                     /locus_tag="VIX62_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018581775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY5860077.1"
                     /translation="MTDAPLKTQIHEQMTTAMKAKDKVEVGTLRMLTSAVRYKEDELG
                     HELSDDEVREVAAKEVKKRTEAADAFREAGRTELAANEEAEREVLVPYAPERLPDAAV
                     DALVDDAIAATGATSMKEMGKVMGIVMGKAKGQVDGGVVQEKVKARLGG"
     gene            complement(3146..5560)
                     /locus_tag="VIX62_07325"
     CDS             complement(3146..5560)
                     /locus_tag="VIX62_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY5860078.1"
                     /translation="MRRASPPRGADPRPFNDRLVFKPLAVVLLVPIVVLSGALTAIIL
                     APPFIGAAMGVMRVDAKLDALGADFTRIPRFPERSTIYANDATTPLATVYLDNRELVR
                     LNNVSNPAKKAVLAIEDSAFYDHGALNWSSLLRAVIENARAGEVVQGGSTITQQLVKN
                     TLGLDPLDRSFERKFQELALAIRAEEKYTKDEIFELYLNQIYLGNGVYGIGTAAKFYF
                     KKPASELTLSEGATLAGMIRRPSFYDPILHPIRTKIRRNDVLNRMIALGEDDDPMGIK
                     RSLGLHTKQQPLELPDGAGEGISQRNQPFFVRYMISQILNNANGSFDSLGKSEKARQR
                     TLFEGGLKIITTLNPKWQNYAQQAADQPYAVYMPNHGSAHPDTSIVSVDTSNGAIRTM
                     LSGKNFHEDKLDLATTQHQPGSSFKPYILAAAFEQGVPPSQSYSSSSPFCSPLWQDDD
                     HCVDNAEGAGSGGKVDLWTATEDSINVVFAQLILDIGPETVPPVANKMGITTELPPVA
                     ALATGSVGVSPLDMAMGYATLANGGIHCSPYTVETILRDNKELYQHEADCERVLKPEI
                     AHLISAMLERVPQSGTAASAFAAGWGDWPVAGKTGTANLNTNVWFCGYTRQVATAVWV
                     GSNGTPYSLGSVFGGTVAAPIWRSYMIRVMDGQPALAFPDPPPPPTSAVPDVVGMTSD
                     EAIAALQAAGFNATVVEVDSLRPKGIVVDQLPAGGAQADLGLSVTIDVSNGKAPESAV
                     PKVVGLSAGTARATLEDAGFVVKVTSKPVKDKKDDGIVLAQSPAGGEPAPKGTTVTIT
                     VGVKGG"
     gene            5612..6964
                     /locus_tag="VIX62_07330"
     CDS             5612..6964
                     /locus_tag="VIX62_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY5860079.1"
                     /translation="MRRTALFLCCFGVFALWLSMSSAMSSAMASGATTADVVVLKVEG
                     AIDRPLLNYLDDRLTSAEADGAIVVLQLDTSGTLDQDGVALGQRLVDMDVPVIAFVGP
                     TTSPTNASGAGLLLMYASSLSAVAPGSQTGPLNPIDLAHPDQAYPGLDQTIQGWIDAR
                     GKDTSLDWDDRPLTAQDAIDLGIATEVAQSVPELLNVIDGRTVQTAGGPVTLHTRIAT
                     TEQEANEGTVNVRFDNLGPIQRVAHGVATPSMIYFLLVLGLACFAFELTQPGFGFAGF
                     AGVGMLGLAAYGLWVAPPWWPGMVLMLLGVGFMMLDVRLRRLGVFTWVGLAGFAGGSI
                     LAWKGVAPAIEISPWLIGGAVVASLLYYGFALTVAIQSRDRIVNTQQGLIGLVGEARG
                     KLAPEGPVYVKGAMWRGRTGGDAIPPGTKVRIRGVDGLVLKVEAEPQEVGAGSGPVDG
                     "
     gene            7067..7336
                     /locus_tag="VIX62_07335"
     CDS             7067..7336
                     /locus_tag="VIX62_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017588640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY5860080.1"
                     /translation="MQNDLAIAWQHLAACRGEDSTYFFAPSYFERRAEKNAREAVAKA
                     ICARCPVRDECLEYALGTRDNHGIWGGMNEMERRAILRQRAREAV"
     gene            7395..7877
                     /locus_tag="VIX62_07340"
     CDS             7395..7877
                     /locus_tag="VIX62_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5860081.1"
                     /translation="MNDAEILQRLSDLSIELPDPPRAVAAYVPVRIAGDVAYVAGQVP
                     VVDGEVVHPGRLGDPDSGPTPEDAALAARQAALQVLSALREGLGGSFERLAGIAQVTV
                     YLATTPDFVEHPQVANGASQLLVDVLGEEGRHARAAVGMASLPLGASVEVAVVASLTR
                     "
     gene            complement(7874..9730)
                     /gene="selB"
                     /locus_tag="VIX62_07345"
     CDS             complement(7874..9730)
                     /gene="selB"
                     /locus_tag="VIX62_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEY5860082.1"
                     /translation="MDGPVAAQSRVVATAGHVDHGKSSLIVALTGMDPDRWDEEKRRG
                     LTIDLGYAWCTLPSGREIAFVDVPGHERFIGNMLAGVGPVRSVLFVVAADEGWKPQSE
                     EHLQILDVLGAGSAVVALTKRDLVDEETAAIATEEVRDRLAGTALFDAPIVAVSASTG
                     DGIDALRAALDDMLERVPPPDPARTRLHIDRVFTIKGAGTVVTGTLTGDCLSVGDDVI
                     LLPSNRRARVRSLQSHRTVHDRACPVSRVAINLVGIERDEVARGDVLARPGAWRPTDR
                     FEATLRPVRGLRHDVGARGAYKVYAGTAEVDARIRVYGGGPIASGDDAFVRIHTSSPL
                     ALDVFDRFVVREAGRQETVAGGRVLDVDPPGRAGPAPAERLASWAHAERDALPGLLAD
                     ERGAVRAADAALLTGSGAPAGTVVGSWFVRPSLFDEVERTVTARLRAHHEAHPLEEGA
                     DVGPVRAAVKAVLRADRVPAEPELVDSMIQDLADRNVVVMGGATVRLPDHGVSLAAHD
                     DTVARLLDAIGGEHATMPPTVDELERSGIERSVIEAAARAGLVVQVSPDLIFSPAVVD
                     RAMTVVRAAPDGITVSAFREALETSRKFALPLLEHFDRTGVTRRDGNLRFPR"
     gene            complement(9803..11179)
                     /gene="selA"
                     /locus_tag="VIX62_07350"
     CDS             complement(9803..11179)
                     /gene="selA"
                     /locus_tag="VIX62_07350"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY5860083.1"
                     /translation="MADERRSVPSVDSLLRTPPVRRAADVFGRPLLKYTLTEELAAVR
                     RRAADGHAAPSPEDIVTSAITLASRIATGITPVINATGVVLHTNLGRAPLPSRALEAA
                     VRAGGYGDLEVDRGSGRRGKRSTRAEQLLIALTGAEDAFVVNNCAAALLLSLAALAKG
                     KQVLVSRGELIEIGGEFRIPDIMRASGARLVEVGTTNRTRIGDYRAAVTDRTGAVLKV
                     HPSNYRVVGFTAATPAKELATLARRHEVPLLYDVGSGLLTPGHGMPADEPAVVEALAD
                     GADLVMCSGDKLLGGPQAGLVFGRGDLVARLRKHPIARAVRVDKLQIAALEQVLAMVA
                     RGEDDEIPVHRMLRESAGSVRKRAQRLSESLGGDLEHAHVHRCESVIGGGSMPGTAIA
                     SWGVRLAVPDPTAFAARMRAGSPAAFCRVEDDHVLFDLRTVTEDQVPDLARAILYAIE
                     GDEFLEED"
     gene            11309..12499
                     /locus_tag="VIX62_07355"
     CDS             11309..12499
                     /locus_tag="VIX62_07355"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY5860084.1"
                     /translation="MVDLSDRPAFQSSQVTSMIRGGGLDVEIANPGMATEEMILNIGP
                     QHPSTHGVLRVVLELDGEVITRAEPVIGYMHRAAEKLAEFRDTRQTLVLMNRHDWISA
                     FNNELGFVIAVERLADIDVPERARWIRTMFAEWNRLLSHLMFSGSYPLELGAMTPIFY
                     AFREREMIQDLMESATGARMHHSYCRVGGLKDDLPRGFLKESAVVLAAVRKSIRDFES
                     LIMGNEIIFARCRNVGILPTEVAIAYGTSGPVLQASGVPMDPRKDEPYEKYDEVEFEV
                     PVGQTGDSYDRLWVMIERMKQSCNIIEQCMDKLPPGEFRAAKLPKTLKLDGEVYVRTE
                     NPLGLMGYYLVGDGNKEPYRLKMRTASFSNVSVIPAMLPGTLLPDLISILGSIFFVVG
                     DVDR"
     gene            12539..>13270
                     /locus_tag="VIX62_07360"
     CDS             12539..>13270
                     /locus_tag="VIX62_07360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5860085.1"
                     /translation="MVGDLTPELRVAIERARVIADTVLFPQAQETDTAELVPHGHLDA
                     LREAGLNGLQGPTGFPGGLAADHATARLVFSVVAGGCGATAFVWAQHHGAVRRLTGGD
                     GPGRDRWLPSLCDGSLLAGIGFAYLRRPGPPAVRAVPSGSGWRLEGAAPWITGWGLAD
                     LFVIMARADDGSVVTVVVDEPYERPELIPGPRQRLAVMGSTGTVSLRFDGLEIGETSV
                     AGVQNWSPEPLKEEATRAKDDAGTPR"
BASE COUNT         2036 a         4482 c         4664 g         2088 t
ORIGIN      
        1 ctgcccccgg gtcttggcga tgttcgtcac gatccggaag atccaggtgc gcagcgacga
       61 acgtccctcg aaccggtcga ggcccttcag gacgccgagc caggcatcac ccacgacctc
      121 ttcggcgacg gcctgggagg agacgtacat gcgcgccacg cggagcatcg caggttgcaa
      181 cgcgtcgacc agttccatga aggcgtcttc atcgccggca cggagccgtt cgaggagttc
      241 ggcgtcagtc tcggcgttcg cgggagcgct cacggtgcgt cagcctatcc acccatgctg
      301 ccgcgagact cggaacggag tagcggaggt agtggcggga gctagcccgg tcggacgatg
      361 cgaaccacgg gagtgccgta gccggacacc gatccgtact gcacctggcc accggggccg
      421 ggatggcgtg cgtggatcat ctgaccgcca ccgatgtaga gcgccacatg gctgatcggt
      481 gagtaaaaga acagcaggtc accggcagca gcctgggaga gcgacgggac cacggcaccg
      541 gagtacgcct gggacgccga ggagtgcggg aggtacacgc cggcctgacc ccacgcccat
      601 gaggtcaatc ccgagcagtc gaagcccacg gccgggttgg cggagcccca cacgtacggc
      661 gcgccgatca cgctcttcgc ggctgcgacc gctgcggcgg cgccggatcc gggcggcgga
      721 ggcggcggct cggtccagcc gccggggtcg gtgcttccgc ctccactggt tgtggtgccc
      781 ccggtgctgc cggtgttctg ctcagcagcc gccgcagccg ctgcggcggc ttcctgacgc
      841 tcgaggtagt cctggtagtc cttgttgagc tgctcgtaca gcgcctcctg ctcctgcagc
      901 atcgcctgga tgtcctcgcg gttcgcggac atcttcgaga gctcggcctt ctgctgatcg
      961 agcgcctcgc cgtactgctg cgccgcccag tccgcacgtt ggcgggcgtt ggcggcttgg
     1021 gtggcgaggt cggcgtcgtt ctgcgcgagc gcgcccatga actcgagccg atcggagaac
     1081 tcggtgaggt cttgggcgcc cagcaggacg tcgagctggt tgccggagcc ggtgtaggct
     1141 tcgacggctc gggcctccag gtcctcggtg gcggctgcgg cctcagcctc agcctcctgc
     1201 ttgtcggcga gggcggcgtc gagcttgtcc tggatgccgc cgaggcggac cttcgcctcg
     1261 ttgtactgct cgatcacgat ctcgagcttc tcgctgacgt cctgcagctt cgccttggcg
     1321 gcgtccacct cggccttcga tggcgcggcc gacgaggagg tgacgagaga accgacgagc
     1381 gtggggacca cgaagatgac ggctacggtg agccgtgcaa gtcgcttggt ggtcctggtc
     1441 atgcccctcc atcgggtcgt cggataaatg aaaaggctcc gagcccgttg ccggttgcgg
     1501 agcctacgag caaggaggct aacggccagg tccggaggcg tcaaacttct cgggtcgatc
     1561 ccagccggga agcaaaccgt ttccgcaggt caccaagggt aggcgggggc ctacggaaaa
     1621 tgacggagga cgggcccgta tcgcgcccgt cctccggagc ctcgagtcgg gacggcggga
     1681 tttgaacccg cgacctcagc gtcccgaacg ctgcgcgcta ccaagctgcg ccacgtcccg
     1741 aggaccacag agtgtagcga gtcgaacgcc cgatgggcag gctagggggc cggctcgggc
     1801 cgccggcgca gctccagcat cgtggcctcg ggccggcacc agaaccggaa cggggcgtac
     1861 ttgctggtgc ccatccccgg ggaggtgtgc agcacggccc cgcccatccc gatcacgcct
     1921 ttgaccagcc gccgaggcat ggaacagttc gtcaccagcg cgcccaccac cggcaggcag
     1981 acctgaccac cgtgcgtgtg cccggcgacg atcaggtcgt agccgagcga ggcggcctct
     2041 ggcgccgagt ccggcgagtg catcacggcg aacccgaacc ggtccggcgc ccgcctgggt
     2101 gcatcccggt agtcctgcca ccggatgtgc gcgtcatcga gtccgagtag ctcgaccgcc
     2161 aggccgtcca agtccacgtc gtggcggacg ttcgtgagat cggtccagcc gtccgcgttc
     2221 agctgggtca ccaggtcgcc ggcccgtccc ttcacggcgc tacgacgttt gcggttccgc
     2281 tggaagtacg cgaagtagtt caggggccgc ggcgcgaagt agtcgttcga tcccagcacg
     2341 taccacgagg cgagtcgccc ccgggtgggc cggagcatgt cgaccacggt ctccaccccc
     2401 tcgggctcgg cgaggaagtc ccccgtgacc acggtgacgt cggcgggagg cagcgaggcc
     2461 aggaaccggc gcgacttccc gggttcgtcc cgcacgaaat gcaggtcgga caggtgcagg
     2521 accgacaggg tctcgggacc tgtcgccggt aggatggcga ggcggtgccg caccagccgg
     2581 taccaacggc gttcgatcag gatgccgtag agcacgcagc cgatgcccac cagcgcgact
     2641 gcgagcacga cggggatcac cacgggaagg cagtgtagtg acggacgctc cgctgaagac
     2701 gcagatccac gagcagatga ccacggcgat gaaggccaag gacaaggtcg aggtgggcac
     2761 gctccgcatg ctcacctccg cggtccgcta caaggaggac gagctgggtc acgagctctc
     2821 cgacgacgag gtccgcgagg tcgccgcgaa agaggtcaag aagcgcaccg aggccgcgga
     2881 cgccttccgc gaggccggac gaacggagct agccgccaac gaagaggccg aacgcgaggt
     2941 cctggtgccc tacgcccccg aacggcttcc cgacgccgcc gtcgatgcgc tcgtcgacga
     3001 cgcgatcgcg gcgaccgggg ccacctccat gaaagagatg ggcaaggtca tgggcatcgt
     3061 gatgggcaag gccaagggcc aggtggacgg cggggtcgtc caggagaagg tcaaggcccg
     3121 gctgggcggt tgacgcccgg cacccctagc cgcctttgac gccgacggtg atcgtcaccg
     3181 tcgttccctt cggcgcaggc tcaccgccgg ctggcgattg cgccagcacg atgccgtcgt
     3241 ccttcttgtc cttcaccggt ttggacgtca ccttcaccac gaagccagcg tcctcgagtg
     3301 tggcgcgcgc ggtgcccgcc gacaggccca ccaccttggg tacggcgctc tcgggcgcct
     3361 tgccgttgct cacgtcgatc gtcaccgaca ggcccaggtc cgcctgcgcg ccaccggccg
     3421 gcaactggtc gaccacgatc cccttgggcc gcaacgagtc cacctcgacg acggtcgcgt
     3481 tgaacccggc agcctgcagc gccgcgatgg cctcgtcgga ggtcatgccc accacatcgg
     3541 gcaccgccga ggtgggcggc ggcggcgggt ccgggaaggc gagcgccggc tggccgtcca
     3601 tcacgcggat catgtacgag cgccagatcg gcgccgcgac cgtcccaccg aacaccgacc
     3661 cgagcgagta cggcgtgccg ttggatccca cccacaccgc cgtggcgacc tgccgggtgt
     3721 agccgcagaa ccacacgttc gtgttcaggt tggccgttcc cgtcttgccc gccaccggcc
     3781 agtcacccca tccggccgcg aacgcactgg ccgcggtccc cgactggggg acacgttcga
     3841 gcatggcgct gatcaggtgc gcgatctccg gcttcaggac gcgctcgcag tcggcctcgt
     3901 gttggtagag ctccttgttg tcgcgcagga tcgtctcgac ggtgtagggc gagcagtgga
     3961 tcccaccgtt ggccagggtg gcgtaaccca tcgccatgtc caagggggag acgcccaccg
     4021 acccggtcgc gagcgcggcc accggcggta gctcggtcgt gatgcccatc ttgttggcca
     4081 ccggcggcac ggtctcgggt ccgatgtcca ggatcagctg tgcgaacacg acgttgatgg
     4141 agtcctcggt ggcggtccac aggtccacct tgccgcccga ccccgcgcct tcggcgttgt
     4201 cgacgcagtg gtcgtcgtcc tgccacagcg ggctgcagaa cggtgaggag ctcgagtagc
     4261 tctgcgacgg cggcacccct tgctcgaacg ccgccgccag gatgtagggc ttgaacgacg
     4321 agcccggctg gtgctgggtg gtggccaggt ccagcttgtc ctcatggaag ttcttacccg
     4381 agagcatcgt gcggatcgcg ccgttgctcg tgtcgaccga gacgatcgac gtatcgggat
     4441 gtgcgctgcc gtggttcggc atgtacaccg cgtacggctg gtcggcggcc tgttgcgcgt
     4501 agttctgcca cttcgggttg agggtggtga tgatcttcag cccgccctcg aacagggtgc
     4561 gttgccgcgc cttctcgctc ttgcccagag agtcgaacga gccgttcgcg ttgttcagga
     4621 tctggctgat catgtagcgc acgaagaagg gttggttccg ctgggagatg ccttcgccgg
     4681 cgccgtcggg caactccagc ggttgctgct tcgtgtgcag gcccagggag cgcttgatcc
     4741 ccatggggtc gtcgtcttcg cccagggcga tcatccggtt gagcacgtcg ttgcgccgga
     4801 tcttcgttcg gatggggtgc aagatcgggt cgtagaagga gggccggcgg atcatgccgg
     4861 ccagcgtggc gccttcggac agcgtcagct ccgacgcggg cttcttgaag tagaacttgg
     4921 ccgccgttcc gatcccgtac acgccgttgc ccaggtagat ctggttcagg tacagctcga
     4981 agatctcgtc cttcgtgtac ttctcctcgg cacggatcgc cagggccagc tcctggaact
     5041 tccgttcgaa gctccgatcc aacgggtcga gccccagggt gttcttcacc agctgctggg
     5101 tgatcgtgga accaccctgg accacttcgc ccgcccgcgc gttctcgatg acggcgcgca
     5161 ggagcgagct ccagttcagc gcaccgtggt cgtagaaggc ggagtcctcg atcgccagca
     5221 cggccttctt cgccgggttg ctgacgttgt tcaggcgcac caactcacgg ttgtccaggt
     5281 acacggtggc gagcggcgtg gtggcgtcgt tcgcgtagat cgtggagcgt tccgggaacc
     5341 gcgggatccg cgtgaagtcg gcccccagcg catcgagctt ggcgtcgacc cgcatcacgc
     5401 ccatcgccgc gccgatgaac ggcggcgcga ggatgatggc tgtcagcgct cccgacagca
     5461 cgacgatggg cacgagcagt acgaccgcaa gtggcttgaa caccaggcgg tcgttgaagg
     5521 ggcgtggatc ggccccgcgg ggtggggaag ctcgtcgcat gggatgccgc ccaaggatac
     5581 tccgggcgac ccccaggtac gatgggtggg catgcgacgt acggcccttt tcctctgctg
     5641 tttcggtgtc ttcgcgctgt ggctatccat gtcctcggcc atgtcctcgg ccatggcgtc
     5701 gggggcgacc accgccgatg tcgtggtcct caaggtcgag ggtgcgatcg accgcccctt
     5761 gctcaactac ctcgacgacc gcctgacgtc cgcggaggcc gacggcgcga tcgtggtgct
     5821 gcagctcgac acctccggca ccctggatca ggacggggtg gcactcgggc agcggctcgt
     5881 ggacatggat gtgccggtca tcgcgttcgt aggccccacg acctcgccca ccaacgcgag
     5941 cggcgccggc ctgctgctga tgtacgcctc atcgctgagc gcggtcgccc ccggctcgca
     6001 gacgggaccg ctgaacccga tcgacctggc ccaccccgat caggcgtacc cgggcctcga
     6061 tcagacgatc cagggatgga tcgatgcccg gggcaaggac acctcgctcg actgggacga
     6121 tcgtcccctc acggcgcagg acgccatcga tctcgggatc gccacggagg tggcgcagtc
     6181 ggtccccgag ctcctcaacg tgatcgacgg ccggacggtc caaacggccg gggggcccgt
     6241 cacgctgcac acccgcatcg ccaccaccga gcaggaggcc aacgagggca cggtcaacgt
     6301 ccggttcgac aacctcgggc cgatccagcg cgtggctcac ggggtggcca cgccgtcgat
     6361 gatctacttc ctgctcgtcc tcggcttggc atgcttcgcc ttcgaactca cccagccggg
     6421 gttcgggttc gcggggttcg cgggcgtggg gatgctgggc ctggccgcct acggactctg
     6481 ggtcgcacca ccgtggtggc ccgggatggt gctgatgctc ctgggcgtgg gtttcatgat
     6541 gctcgacgtc cggctccgcc gcctcggggt cttcacatgg gtgggtctgg caggattcgc
     6601 cggtggctcg atcctggcgt ggaagggcgt cgcgcccgcc atcgagatct ccccgtggct
     6661 gatcggcggc gccgtcgtgg cgagcctcct ctactacggg ttcgcactca cggtggcgat
     6721 ccagtcgcgt gatcgcatcg tgaacaccca gcagggcctg atcggcctgg tcggcgaggc
     6781 ccggggcaag ctcgcgcccg agggtccggt ctacgtcaag ggcgcgatgt ggcgcgggag
     6841 aacgggcggc gacgcgatcc cgcccggcac gaaggtgcgg atccgagggg tcgacggcct
     6901 ggtgctcaag gtcgaggccg agccgcagga agtcggcgcc gggtccgggc cggtcgacgg
     6961 ctgaccgtcg accccggccc caggtcgtgg cgatctcgtc acagtgccgc tgcatcgtct
     7021 cgcgggcgaa tccccgaagc cgtcgctttc gaacaacccg ataggcatgc agaacgacct
     7081 cgccatcgcg tggcaacacc tcgccgcctg ccgcggcgag gattccacct acttcttcgc
     7141 ccccagctac ttcgagcgac gcgcggagaa gaacgcccgc gaggccgttg ccaaggcgat
     7201 ctgcgcccgc tgccccgtgc gagacgagtg cctggagtac gccttgggca cgcgggacaa
     7261 ccacgggatc tggggcggca tgaacgagat ggaacgccgc gcgatcctgc ggcagcgcgc
     7321 ccgcgaagcc gtctgagact gcaggcgacg tcgcgagtcg cggcccgctc gatcctcgtg
     7381 cgaggatgct cgccatgaac gacgccgaga tcctccaacg cctgtccgac ctgtcgatcg
     7441 agctgcctga cccacccagg gccgtggccg cgtacgtgcc ggtgcgcatc gcgggcgacg
     7501 tggcttacgt ggccggccag gtacccgtgg tcgacgggga ggtggtgcat cccggacggc
     7561 tcggtgatcc ggactccggc ccgacccccg aggatgccgc gctcgcagcg cggcaggccg
     7621 ccctgcaggt gctctcggcg ctgcgggaag ggctcggcgg cagcttcgaa cgcctcgccg
     7681 ggatcgcaca ggtcacggtg tacctcgcga cgacccccga cttcgtcgag catccacagg
     7741 tggccaacgg tgccagccag ctgttggtcg acgtgctggg cgaggagggc cggcacgctc
     7801 gagccgccgt cgggatggcc tccctgccgc tcggcgcgag cgtcgaggtg gcggtggtgg
     7861 cctcgctgac ccgctagcga gggaaccgaa ggttgccgtc tcggcgcgtg acgccggtcc
     7921 ggtcgaagtg ttcgagcagg ggcagcgcga acttgcggct cgtctcgagc gcctcacgga
     7981 acgcgctcac cgtgatgccg tcgggtgccg cgcgcacgac cgtcatcgcc cggtccacga
     8041 cggcggggct gaagatcagg tcgggcgaca cctggacgac gagaccggcc cgtgccgcgg
     8101 cctcgatcac gctgcgttcg atgccggatc gctcgagctc atcgaccgtg ggcggcatcg
     8161 tggcgtgctc gcccccgatg gcatcgagca gtcgcgcgac cgtgtcgtcg tgcgcggcga
     8221 gcgagacccc gtgatcgggc aggcgcaccg tggcgccgcc catcaccacg acgttccgat
     8281 cggcgaggtc ctggatcatc gaatcgacga gttcgggctc ggccggcacg cgatccgcgc
     8341 gcaacaccgc cttcaccgcg gcgcggacgg ggccgacatc ggcgccctcc tccagcgggt
     8401 gggcctcgtg gtgggcgcgg aggcgcgcgg tgacggtgcg ttcgacttca tcgaacaggg
     8461 agggacgcac gaaccagctg ccgacgacgg tgccggccgg ggctccggac ccggtgagca
     8521 acgcagcatc ggccgcccgg acggcgccgc gttcgtcggc gagcaggccg ggcaaggcgt
     8581 cccgctcggc gtgtgcccac gatgcgagcc gctcggcggg tgcaggtccg gcgcggcccg
     8641 gagggtcgac atcgagcacc cggccgccgg ccacggtttc ctgcctcccc gcctcgcgca
     8701 cgacgaagcg atcgaagacg tcgagggcca gcggcgagga cgtgtggatc cggacgaacg
     8761 cgtcatcgcc ggaggcgatc ggcccgccac cgtagacacg gatccgcgcg tcgacctcgg
     8821 ccgtgccggc atacaccttg taggcgccgc gggcaccgac gtcgtgtcga aggccacgca
     8881 ccggccgaag ggtcgcttcg aaccggtcgg tgggccgcca ggcaccgggc cgggccaaga
     8941 cgtcgccgcg tgcgacctcg tcgcgttcga taccgaccag gttgatcgcc acccgggaca
     9001 cggggcacgc gcgatcgtgc acggtgcggt ggctctggag cgaacggacg cgcgcgcgac
     9061 ggttcgacgg caagaggatg acgtcgtcgc cgaccgagag gcagtcgccg gtgagggtgc
     9121 cggtcaccac cgtgcccgcg cccttgatcg tgaagacccg gtcgatgtgg aggcgcgtcc
     9181 gagcggggtc gggcggcggg acacgctcga gcatgtcgtc gagcgccgcc cggagtgcgt
     9241 cgatgccgtc gccggtcgat gccgagacgg ccacgatcgg cgcgtcgaac aaggccgtgc
     9301 cggcgagccg atcgcgcacc tcctcggtgg cgatcgcagc ggtctcctcg tcgacgaggt
     9361 cccgcttggt cagcgccacg accgcggaac cggcgcccag gacgtcgagg atctgcagat
     9421 gctcctccga ctggggcttc catccttcgt cggcggccac cacgaacagc accgaccgga
     9481 ccggaccgac gcccgccagc atgttgccga tgaagcgctc gtggcccgga acatccacga
     9541 aggcgatctc gcgaccgctc ggcagcgtgc accacgcgta cccgaggtcg atcgtgagcc
     9601 ctcggcgctt ctcctcgtcc cagcggtccg ggtccatccc ggtgagggcg acgatcagcg
     9661 aggacttgcc gtggtcgacg tgtccggcgg tcgccacgac ccgtgactgc gcagcgaccg
     9721 gtccgtccat gcgggccatc ctacggctgc gcctgtctcg atccctcggc tgggtggaca
     9781 ccgaaatggc acgaaccctg ggtcagtcct cctcgaggaa ttcgtcgccc tcgatggcat
     9841 acaggatcgc tcgcgccaga tcgggcacct gatcctcggt caccgttcgc aggtcgaaca
     9901 acacgtggtc gtcctcgacc cggcagaacg cggccgggct gccggcccgc atccgggcgg
     9961 cgaaggcggt cgggtccggc accgcgagtc gcactcccca cgaggcgatc gcggtcccgg
    10021 gcatggatcc cccgccgatc acggattcgc agcggtgaac gtgcgcgtgc tcgaggtcgc
    10081 cgccgaggct ctcggagagt cgctgcgcac gcttgcggac cgacccggcc gattcgcgca
    10141 gcatgcggtg gacggggatc tcgtcgtcct caccacgcgc gaccatcgcg aggacctgtt
    10201 cgagggcggc gatctgcagc ttgtccacgc ggaccgcgcg tgcgatcgga tgcttgcgga
    10261 gcctggcgac caggtcgccg cgaccgaaca ccagtcctgc ctgcggtccc ccgaggagct
    10321 tgtctccgga gcacatgacg agatcggcgc cgtcagcgag ggcctcgacc acggcgggct
    10381 cgtcggccgg catcccgtgg ccaggggtga gcagtcccga tccgacgtca tacagcaggg
    10441 ggacctcgtg ccggcgtgcg agggttgcga gttccttcgc aggggtcgcc gccgtgaacc
    10501 ccacgactcg gtagttcgaa gggtgcacct tgaggaccgc gcctgtgcgg tcggtcaccg
    10561 cggctcggta gtcgccgatg cgcgtccggt tggtggtgcc cacctcgacc agtcgcgctc
    10621 cggaggcacg catgatgtca gggatcctga actctccgcc gatctcgatc agctccccgc
    10681 gggagaccag cacctgcttg cctttcgcca gcgcggcaag gctgaggagc agcgcggcgg
    10741 cgcagttgtt gaccacgaag gcgtcttctg cgcccgtgag ggcgatcagc agttgctcgg
    10801 ctctagtgga gcgcttgcct cggcgacccg aaccgcggtc gacctctagg tcgccgtagc
    10861 cacctgcccg cacggcggcc tcaagcgcgc gggaaggcaa gggggcacgt ccgaggttcg
    10921 tgtgcaggac gaccccggtg gcgttgatca ccggtgtgat gcccgttgcg atccgggacg
    10981 ccagggtgat ggcggaggtc acgatgtcct cgggggacgg cgccgcgtgg ccgtcggccg
    11041 ctcgtcgccg gaccgctgcg agttcctcgg tgagcgtgta tttcaggagt gggcggccga
    11101 acacgtcggc cgcgcgtcga accggcgggg tccgcaggag cgaatctacc gagggcacgg
    11161 agcgacgctc gtcggccatg ccgaggagcc taccggttga acgacggtca acgctgtggg
    11221 tgcccaagac atgggagacc gccgacatcg accacctggc ggcgtgtcac ctggcccaga
    11281 tctcggaccg cccgctagga taggaacgat ggttgatctg tccgatcgcc cggcattcca
    11341 gagctcccag gtcacgagca tgatccgggg aggtggtctc gacgtcgaga tcgcgaaccc
    11401 gggcatggcg accgaggaga tgatcctcaa catcgggccg cagcacccgt cgacccacgg
    11461 cgtgttgcgc gtggtcctcg aactcgacgg cgaggtcatc accagggcag aacccgtgat
    11521 cggctacatg caccgcgcag cagagaagct cgctgagttc cgcgacactc ggcagacgct
    11581 ggtgttgatg aaccgacacg actggatcag cgcgttcaac aacgagctgg ggttcgtgat
    11641 cgccgttgag cgcctcgccg acatcgacgt tcccgagcgg gcccggtgga tccgcacgat
    11701 gttcgcggag tggaaccgcc tgctgagtca cctcatgttc agcggctcgt atccgttgga
    11761 actgggggcc atgacgccga tcttctacgc gttccgtgag cgcgagatga tccaggacct
    11821 gatggagagc gccaccggcg cgcgcatgca ccactcgtac tgccgtgttg gcggtctcaa
    11881 ggacgatctg ccccgcgggt tcctcaagga gagcgccgtg gtgctcgccg cggtgcgcaa
    11941 gtcgatccgc gacttcgaat cgctgatcat gggcaacgag atcatcttcg cgcggtgccg
    12001 caacgtgggc atcctgccca ccgaggtcgc catcgcctac ggcaccagtg gcccggtcct
    12061 gcaggccagt ggggtgccca tggatccgcg caaggacgag ccgtacgaga agtacgacga
    12121 agtggagttc gaggtcccgg tgggccagac cggcgacagc tacgaccgcc tctgggtgat
    12181 gatcgagcgc atgaagcaga gctgcaacat catcgagcaa tgcatggaca agctgcctcc
    12241 cggtgagttc cgggcggcga agctgcccaa gacgctcaag ctcgacggcg aggtctacgt
    12301 gcgcaccgag aaccccctcg gcctcatggg ctactacctg gtgggtgacg gcaacaagga
    12361 gccgtatcgc ctcaagatgc gcaccgcgtc gttctccaac gtcagcgtga tcccggccat
    12421 gttgcccggc accctgctgc ccgacctgat ctcgatcctg ggatcgatct tcttcgtggt
    12481 cggcgacgtc gaccgctgac ccctccatcg tcggtcggct ccccgctacg ctcacgtcat
    12541 ggttggtgac ctgacgcccg aacttcgggt cgcgatcgag cgtgcccggg tcatcgccga
    12601 caccgtgctc ttcccgcagg cccaggagac cgatacggcg gagctcgtgc cccatgggca
    12661 tctggatgcg ctacgcgaag ccgggctgaa cggcctccag ggtccgaccg ggttcccggg
    12721 tgggctcgcc gcggatcatg cgaccgcccg gctcgtgttc tcggtggtcg ccggcggctg
    12781 tggggcgacg gcgttcgtgt gggcgcagca tcacggtgcg gtccggcgac tgacgggcgg
    12841 tgacggtcca ggccgcgacc ggtggctccc gtcgttgtgc gacggatcgc tcctggccgg
    12901 gatcgggttc gcgtacctgc gtcgtcctgg ccccccggcg gttcgcgcgg tgccgtccgg
    12961 atcggggtgg cggctggagg gggcggcacc gtggatcacg gggtgggggc tggcagacct
    13021 gttcgtgatc atggccagag ccgacgacgg cagcgtggtc accgtcgtgg tcgacgaacc
    13081 gtacgagcgt ccggagctga tccccggccc gcgtcaacgc ctcgccgtga tggggtcaac
    13141 gggcacggtc tcgcttcggt tcgacgggct cgagatcggt gagacctcgg tcgccggcgt
    13201 gcagaactgg agcccggaac cgctcaagga agaggcgact cgtgcgaaag acgacgcagg
    13261 gacgccgcgc
//
LOCUS       DATDBZ010000301         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_920690, whole genome shotgun sequence.
ACCESSION   DATDBZ010000301 DATDBZ010000000
VERSION     DATDBZ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920690"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..144)
                     /locus_tag="VIX62_07365"
     tRNA            complement(61..144)
                     /locus_tag="VIX62_07365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Leu,seq:taa)
     gene            complement(207..533)
                     /locus_tag="VIX62_07370"
     CDS             complement(207..533)
                     /locus_tag="VIX62_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860086.1"
                     /translation="METTTTVQFADAARSLSRAARSRGLGAPTFRSPPRVVGVDRSLR
                     RHGDHAVVAVRLRGRPWVAVAADMVEGVIVANRLEGPAALRVRGALWSALGFSTASDE
                     SQSTAA"
     gene            670..1602
                     /locus_tag="VIX62_07375"
     CDS             670..1602
                     /locus_tag="VIX62_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY5860087.1"
                     /translation="MVLNPAQQSVIDLLRRSADRVVPPPELATELEAELNDDLSDLAD
                     QIDPAVPLWVSKHALTTIHGCETHHMAGQGSFEWNVASARGTVSHKAIEVLVNYRGDP
                     FAPDLVDEAMSRLINDEHADIGRFIAALPEFDRAELRALAVDRVTAFQESFPPLKPQW
                     TPRTESRSRVELLDGRVVLSGKSDLTLGRPGDKVIIDLKTGRPSRTHREDLRFYALLE
                     TIKLGAPPRKVASYYLDAARTDPEDVTEGTLRAAARRTSDGVRRIIAVTRLGEDATKR
                     PGPHCRWCPVLPNCTEGTAALARADDPEDDEPPW"
     gene            complement(1633..>2259)
                     /locus_tag="VIX62_07380"
     CDS             complement(1633..>2259)
                     /locus_tag="VIX62_07380"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEY5860088.1"
                     /translation="VVRRARLGAMTDVTADGAAPTLVGISFSDAFRAREFLSAATRLA
                     AIGQLKLKDAVFVSKDAEGQTRVQETMDPGPGRSALSGAAWAGLLGLILGGPVGWAIG
                     AGVGAGTGAITAKVIDHGIPDEWVHWFREAVQPNTTTLALLLEDADPGALVEEARRFA
                     GAHLVYANLPPAWENRIREALGEPSSPTGTDPAGTGAPLPPPQPLPGT"
BASE COUNT          382 a          804 c          748 g          325 t
ORIGIN      
        1 acctttcaac tgccgacgaa cgccgagagg cctcgtatgt gccgtgagca ggatgtgttg
       61 tgcccggggc ggggttcgaa cccgcacccc cttgcgggga ggggggttta agccccccgc
      121 gtctgccagt tccgccaccc gggcgtgtac gaacgttaga tggctgacga gcctcgcgtc
      181 atggagctcc ggggacagga cggcgttcaa gccgcagtgg actgagactc gtcggaggcg
      241 gtggagaacc cgagagccga ccagagcgcg cctcggacgc gcaacgcggc cggtccctcg
      301 agccggttgg cgacgatcac tccttcgacc atgtccgccg ccaccgcaac ccacggtcga
      361 ccacgcaacc ggacggccac caccgcgtga tccccatggc gccggagcga cctgtccaca
      421 ccgaccaccc gcggcgggct gcggaacgtc ggcgcgccga gcccgcggga ccgggcggcg
      481 cgggacagcg aacgggcggc gtctgcgaac tggacggtgg tcgtggtttc catacgccat
      541 cactgtgcct caggggtgtc acagggtttc cgtgtgctgc ctgatcgcga ccgtcgcgga
      601 actccgacat accccacctc tacgctccga aacgatgatg accctcgcca acccctcgag
      661 cggcagccgg tggtgctgaa cccggcccag caatcggtga tcgacctcct gcgccgctcc
      721 gcggatcgag tggtcccccc tcccgaactc gcgacggagc tcgaggccga actgaatgac
      781 gacctcagcg acctcgccga tcagatcgat cccgccgtgc cgctctgggt cagcaagcat
      841 gcgctcacca cgattcatgg gtgtgagacg caccacatgg ccggacaggg atcgttcgag
      901 tggaacgtcg cctcggcccg cggcactgtc tcccacaagg cgatcgaggt gctcgtgaac
      961 taccgcggcg atccgttcgc gcccgacctc gtcgacgagg cgatgagcag gctgatcaac
     1021 gacgagcatg ccgacatcgg gcgcttcatc gccgcgctgc ccgagttcga tcgagccgag
     1081 ctgcgggcgc tcgcggtgga ccgtgtcact gcgttccagg agtcgttccc acccctgaaa
     1141 ccccaatgga ctcctcgcac cgagagccgc tcgcgagtgg aactgctcga cggacgcgtc
     1201 gtgctctccg gcaagagcga cctcacgctc gggcgaccgg gcgacaaggt gatcatcgat
     1261 ctgaagaccg ggcgtccgtc gcgcacgcat cgcgaagacc tccggttcta cgcactgctc
     1321 gagaccatca agctcggcgc acccccgcgg aaggtggcga gctactacct cgacgcagcg
     1381 aggaccgatc cggaagacgt caccgaggga accctgcgag cggcggccag gcgcacgagc
     1441 gacggtgtcc gccggatcat cgccgtcacg cgtctcggcg aggacgccac gaagcgcccc
     1501 ggtccacact gtcgttggtg tcccgtgctg cccaattgca cagaaggcac tgcggcgctc
     1561 gcacgagcgg acgatccgga ggacgacgag ccaccgtggt gacggggcca atccgttcca
     1621 ccagttcggc gatcaggtcc ccggaagcgg ttgcgggggc ggcagtggcg cgccggtacc
     1681 agcggggtcc gtccccgtcg gcgaagacgg ctcgccgagc gcttcacgga ttcggttctc
     1741 ccacgccggg ggcaggttcg cgtagacgag atgggcaccg gcgaaccgac gggcctcctc
     1801 gacaagggcg ccggggtcgg catcttcgag cagcaacgcc agggtcgtcg tgttcggctg
     1861 caccgcttcg cggaaccaat gaacccactc gtcggggatc ccatggtcga tcaccttcgc
     1921 cgtgatcgcg ccggtcccgg caccgacccc ggcaccgatg gcccagccga ctggcccacc
     1981 gaggatcagc ccgagcaggc ccgcccatgc cgcgccggac agcgcggagc gaccgggacc
     2041 cgggtccatc gtctcctgca cgcgggtttg tccttcggcg tccttgctca cgaacaccgc
     2101 gtccttgagc ttgagctgcc cgatcgcggc cagtcgagtc gctgcgctca ggaactcccg
     2161 ggctcggaag gcgtcgctga acgagatccc gacgagcgtc ggggcagcgc catcggcagt
     2221 cacgtcggtc atggcaccca gcctggcacg gcgaacgac
//
LOCUS       DATDBZ010000302         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_721247, whole genome shotgun sequence.
ACCESSION   DATDBZ010000302 DATDBZ010000000
VERSION     DATDBZ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721247"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VIX62_07385"
     CDS             complement(<1..520)
                     /locus_tag="VIX62_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5860089.1"
                     /translation="MATVHDELRARLGTVGAWTFAFDAQPVADVRAGASALEELGYTA
                     LWVPEGSGSRDVLAHLSLLLEATDHVTVASGIANVTARQPDVLQAGMVTLADAFGDRP
                     VFGIGIGHEYSTGTRGIDWDRPLARMRTYLDRMDAATSLPTPATPPRRLLAALGDGML
                     RLSSERAQGAHTY"
     gene            complement(613..>2003)
                     /locus_tag="VIX62_07390"
     CDS             complement(613..>2003)
                     /locus_tag="VIX62_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5860090.1"
                     /translation="ALAGLAGLVAFAGVGILVTKWMRGGWMQSHARPEGSSLSSEGGF
                     WDHVTSVVMGRPVVWLVLGALFMLALSLPYWFQAHPDGEGRGIKTGLAGISTLPDGIQ
                     TKEAFELLVEKFPKAGLEATADIVIEADGVGTPGAQPGAPGELASEYAAAVDTLESSI
                     ADDPNLGTPAPPQVSADGTVALVSIPLAGEATDSQGEAAVATIASLRDTYVPEAFEGT
                     SATVLVGGDTAFVKDFFDISDQYTPLIILVVLGLSFILLTIVFRSIVVPVKAIIMNLL
                     SVGAAYGLIVLVFQKGGPAIGETIANFLGFQQVDAIEAWLPLFLFSILFGLSMDYHVF
                     LLTRIREQYDKTGDNAASVAYGLRTTGGIITGAAIIMVAVFAGFAAGRLTSLEQMGFG
                     LAVAVFLDATVVRSILVPSTMRLLGDRNWYLPRWLQWLPKIDVEGHDAATATITVPDS
                     PAELVEAGDTKE"
BASE COUNT          350 a          707 c          660 g          286 t
ORIGIN      
        1 aatacgtatg cgcaccctgc gctcgttcgc tcgagaggcg cagcatgccg tcgcccaacg
       61 ccgccagcag ccgccgaggc ggcgtggcgg gggtcgggag cgacgtcgcc gcatccatgc
      121 ggtcgaggta ggtgcgcata cgcgcgagcg gccggtccca gtcgatgccg cgcgtgcctg
      181 tcgagtactc gtgaccgatc ccgatgccga acacgggccg atctccgaac gcgtcggcga
      241 gcgtcaccat cccggcctgc aggacatcgg gttggcgcgc cgtgacgttc gcgatgccgc
      301 tggccaccgt gacatggtcg gtggcttcca gcaggagcga caggtgggcc agcacatcac
      361 gggagcccga gccttccgga acccagaggg cggtgtaccc gagctcctcc agggccgagg
      421 ccccggctcg tacgtcggcg accggctgcg cgtcgaaggc gaacgtccac gcccccacgg
      481 tcccgagccg cgcacgcagc tcgtcgtgca ccgttgccat cacgcctccc cgtcgcgggg
      541 gtcaggatac ggcgaagcgg aagagggcac gggcaccctc ttccgcctcg cctgcggatc
      601 gaccgcgaac ggctactcct tcgtgtcgcc cgcctcgacc agctcggccg gcgagtcggg
      661 aaccgtgatc gtcgccgtgg cggcgtcgtg cccctccacg tcgatcttcg gcaaccactg
      721 cagccaacgc ggcaggtacc agttgcgatc gccgagcagc cgcatcgtgg acgggaccag
      781 gatggaccgc accacggtgg cgtccaggaa gaccgccacg gccaggccga agcccatctg
      841 ctcgagcgat gtcagccgcc cggcggcgaa tccggcgaac accgcgacca tgatgatggc
      901 cgcaccggtg atgatgccgc cggtggtgcg aagtccgtag gcgaccgacg cggcgttgtc
      961 gcccgtcttg tcgtactgct cacggatccg cgtcaacagg aacacgtggt agtccatcga
     1021 gagcccgaac aggatcgaga acaggaacag cggcaaccac gcttcgatcg catcgacctg
     1081 ttggaagccg aggaagttcg cgatcgtctc gccgatcgcc ggcccgccct tctggaagac
     1141 cagcacgatg agaccgtagg cggcacccac cgacagcagg ttcatgatga tcgccttcac
     1201 cggcaccacg atcgagcgga acacgatcgt gagcaggatg aacgacaacc ccaacaccac
     1261 caggatgatc agcggtgtgt actgatcgga gatgtcgaag aagtccttca cgaacgccgt
     1321 gtcaccgcca accagcacgg tggcactggt tccctcgaac gcctcaggga cgtaggtgtc
     1381 gcgcagcgac gcgatggtcg ccaccgccgc ctcgccctgg gaatcggtgg cctcgcccgc
     1441 gagtgggatc gacaccaggg ccaccgtgcc atcagcgctc acctgaggcg gcgccggggt
     1501 tcccaggttg ggatcgtcgg cgatcgagga ctccagcgtg tccaccgcgg cggcgtactc
     1561 cgatgcgagc tcgcccggtg ctcccggttg cgcgccgggc gtgccgacgc cgtccgcctc
     1621 gatcacgatg tcggccgtcg cctcgagccc tgccttcggg aatttctcca ccagcagttc
     1681 gaaggcttcc ttggtctgga tcccgtcggg cagggtactg atccccgcca ggccggtctt
     1741 gatcccgcgg ccttcgccgt caggatgtgc ctggaaccag tagggcagtg agagcgcaag
     1801 catgaacagc gcgccgagga cgagccacac gaccggacgg cccatcacga cgctggtgac
     1861 gtggtcccag aagccgccct cggaactgag cgaggaccct tcaggacgtg cgtgcgactg
     1921 catccagccg ccgcgcatcc acttggtcac caggatgccg acaccggcga acgccaccag
     1981 gccggccaga ccggccagcg ccg
//
LOCUS       DATDBZ010000303         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1326846, whole genome shotgun sequence.
ACCESSION   DATDBZ010000303 DATDBZ010000000
VERSION     DATDBZ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326846"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VIX62_07395"
     CDS             <1..286
                     /locus_tag="VIX62_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860091.1"
                     /translation="AHLVVHLDRLTGDDVAIVDLLANTCQRLWLRRGVMEIVGMREPG
                     SGSRSSPEATDEVRDVRGEPSRALASDREPDLSGSDRRCSTWLRAATDAE"
     gene            460..2001
                     /gene="asnB"
                     /locus_tag="VIX62_07400"
     CDS             460..2001
                     /gene="asnB"
                     /locus_tag="VIX62_07400"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY5860092.1"
                     /translation="MAYGTIDPEKCERMLARIHHRGPDDTGRIQLESAWLGHQRLSIM
                     DVTGGKQPIADDTGSAYVVGNGEIYNHEDIRDVLSEVRFATDSDTESAFRLVLRDGSA
                     ALYEVRGMFALAMAHTDGRGAVARDPIGIKPLYWARLGETTIFASELRAFDPEDQPYV
                     EAFPPGHVWTPGIAGHDVAEGAMVRFADAVPVEVRPARLAPDKQWTEEDLGHIRDVLS
                     DSIRRHMMSDVPVGVFLSGGLDSAIVAAIAAKVAGEKGERLPTFAVGTEGSGDLLAAR
                     AVAEYLGTEHHEIVPTAEDLESALDEAVTVIEHFDPALVRSAVPNLLLAREAVKHVKV
                     VLTGEGADELFAGYEYVHAEEFSTPDTLQAELVRSIEGLHELNLQRCDRATMNYGLEA
                     RVPFLDATVMREMLALPAEWKLKIHERTEKQLLREAFDGMVPYDIIWRGKEQFGDGSG
                     AGDVLAKLVEKEKAKEDAQDAAPPTVEHGWELRSDEEIAYYRTWHRSFSGVRPNATLG
                     AFATA"
     gene            complement(2026..>2297)
                     /locus_tag="VIX62_07405"
     CDS             complement(2026..>2297)
                     /locus_tag="VIX62_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY5860093.1"
                     /translation="PRGLASLVFALLALEELGPGADHAVAAIAVTVLLSVVAHGASAA
                     PLAARYGASAAREAEPDAPDVPVRGLPGRRAPEPPSDDGSGDTER"
BASE COUNT          358 a          725 c          870 g          344 t
ORIGIN      
        1 ggcacacctg gtcgttcatc tggaccggct gaccggtgac gacgtcgcga tcgtggacct
       61 ccttgcgaac acctgccagc gattgtggct gcgacggggt gtcatggaga tcgtcggcat
      121 gcgggagccc gggtcgggct cgcggagttc gcccgaagcg accgacgagg ttcgagacgt
      181 ccgcggtgag ccgtccaggg cgctcgcatc cgaccgcgaa cctgatctct ccggttccga
      241 ccgacggtgc tccacctggt tgagggctgc cactgacgcc gaatgatcga cacctgggcg
      301 tgtgaccgaa cccccactca ccgggccggg atccgtggga tcattgaatc tcagctcatc
      361 ggatagctga tcgatcgcga gcctcgatgg acggcgtgtc ccgaccgcga tcagaaatcc
      421 cgatcaagga tgtgaacccg cctcgtgtgc ggaatcgtga tggcgtacgg aaccatcgac
      481 ccggagaagt gcgagcggat gctcgctcga atccaccacc gaggaccgga tgacaccggc
      541 cggatccagt tggagagcgc gtggttgggc caccagcgtc tgtcgatcat ggatgtgacg
      601 ggcgggaagc agccgatcgc cgacgacacg ggttcggcgt atgtggtcgg caacggcgag
      661 atctacaacc atgaggacat ccgggatgtg ctctcggagg tccgtttcgc cacggactcg
      721 gacacggagt cggcgttccg gctggtgctg cgtgatggtt cggcggcgtt gtacgaggtg
      781 cggggcatgt tcgcgttggc gatggcccac accgacgggc gcggcgcggt ggcccgggat
      841 ccgatcggga tcaagccgtt gtactgggcg cggctggggg agacgacgat cttcgccagc
      901 gagctgcgcg cgttcgaccc cgaggaccag ccctacgtcg aggcgttccc gcccgggcac
      961 gtgtggaccc cggggatcgc cgggcacgac gtcgccgagg gcgcgatggt ccggttcgcc
     1021 gacgcggtgc cggtggaggt ccgcccggcg cggctggcgc cggacaagca gtggaccgag
     1081 gaggacctcg gccacatccg tgacgtgctc tcggactcga tccgccggca catgatgagc
     1141 gatgtcccgg tgggggtgtt cctctccggt ggtctggact cggcgatcgt ggcggcgatc
     1201 gcggcgaagg tcgcggggga gaagggtgag cggctgccga cgttcgccgt gggcaccgag
     1261 ggctcgggtg atctgctggc ggcccgggcg gtggcggagt acctggggac cgagcaccac
     1321 gagatcgtcc cgaccgcgga ggacctcgag tccgcgctgg acgaggccgt gacggtgatc
     1381 gagcatttcg atccggcgct ggtgcgttcc gcggtgccga atctgctgct ggcccgcgag
     1441 gcggtgaagc acgtgaaggt ggtgctcacc ggggagggcg ccgatgagct gttcgcgggc
     1501 tatgagtacg tgcacgccga ggagttctcg accccggaca cgctgcaggc cgagctggtg
     1561 cgttcgatcg agggtctgca cgagctgaac ctgcagcgct gtgaccgggc gacgatgaac
     1621 tacgggctcg aggcgcgggt gccgttcctc gacgccacgg tgatgcgcga gatgctggcg
     1681 ctgcccgcgg agtggaagct caagattcac gagcggaccg agaagcagct gctgcgtgag
     1741 gcgttcgacg ggatggtgcc ctacgacatc atctggcgcg gcaaggagca gttcggtgac
     1801 ggctccggcg ccggtgacgt gctcgccaag ctggtggaga aggagaaggc gaaggaggac
     1861 gcgcaggacg cggcgccccc gaccgtggag cacggctggg agctgcgcag cgacgaggag
     1921 atcgcctact accggacctg gcaccggtcg ttctccggcg tccgccccaa cgccaccctc
     1981 ggcgccttcg ccaccgcgta accctccggc gaaccacgcc cgcgatcacc gctcggtgtc
     2041 cccggagccg tcgtcggacg gcggctccgg tgcccgccgg cctggtagcc cccgaacggg
     2101 gacgtcgggc gcgtcgggct cggcctcgcg ggcagcggac gccccgtacc gggcagcgag
     2161 gggagcggcg ctggcgccgt gcgcgacgac gctcagcagc acggtcaccg cgatggcggc
     2221 cacggcgtga tcggcgcccg gaccgagttc ctccagcgcg agcagggcga acacgagcga
     2281 ggcgagtccg cgtgggc
//
LOCUS       DATDBZ010000304         2718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2763364, whole genome shotgun sequence.
ACCESSION   DATDBZ010000304 DATDBZ010000000
VERSION     DATDBZ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763364"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VIX62_07410"
     CDS             <1..782
                     /locus_tag="VIX62_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5860094.1"
                     /translation="DELELWFQPNMLLADGSVAGVEALVRWTHPTRGLVSPSDFIPLA
                     EETGLIVPIGRWVMHEAARYAVELQERYPMQPLFHMAVNLSARQIARPELIEEVREIL
                     LETGLEPSTLILEITESVMMQDMELSIERLGSLKELGVMLAVDDFGTGYSSLNYIRRF
                     PVDILKVDKSFVDGVSEGGEASALTAAVIELAGILNLRPVAEGIERADQLARLLELHC
                     DMGQGFFFAKPLPSEELQTLLTERVSMQAEADALAQGMTQS"
     gene            793..1986
                     /locus_tag="VIX62_07415"
     CDS             793..1986
                     /locus_tag="VIX62_07415"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY5860095.1"
                     /translation="MRRLRPILLAIVVTTGVVATAPPTLAAPPKWVQRIDDVVGRDAV
                     SVTIGYEGTALYRHKDWVRRAPASNEKLLLSMALLDQVPPDTTIPTRVLATKAVRGDG
                     TLRGDLWIVGHGDPETDRQDLAALAADLRERGVERVRGRVMGSTGPFGRDWFAPGWKS
                     YFPRYYIALPTALTYHYNRGPRGTNIRDPERRAATELTKQLQQRGIKVRGKPGMGVPP
                     GNLVGLATRLSEPFVSIMRRMNVFSSNFRAEVFGKFLGARVGGRGTIARGARGIEAFT
                     RAHGVGVEANDGSGLSYANRVSTEGIVELLWVADASPWGETLRGTLAQGGQGTLEDRL
                     RDVKIRAKTGTLTDISALSGWVWLEREDAWAEFSILSQGMSKTRSIQIENAIVRVVNA
                     NAAPR"
     gene            complement(2003..>2718)
                     /locus_tag="VIX62_07420"
     CDS             complement(2003..>2718)
                     /locus_tag="VIX62_07420"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEY5860096.1"
                     /translation="YSGRYAFTHKAGLHTSGVARVSRAYEHIEPETVGNRRGVVASDY
                     GGGATIRMKAAEVGLELTDAAIPVTVDLIKDREAEGYAFDVAEASLELLMRRANGWTQ
                     PFFEIESYRVHVEERMIDDEPPLAEATVKVRTKGTRHIESAEGAGPVAALDDALRKAL
                     VNDYPELTHIGLEDFRVRVLGETTGTGAVVRVLIDTSNGEREWTTVGVSENILEASWE
                     ALVEAYTFGLLHPRERAED"
BASE COUNT          420 a          940 c          929 g          429 t
ORIGIN      
        1 gggacgagct ggagctgtgg ttccagccca acatgctcct cgccgacggc agcgtggccg
       61 gcgtggaggc cctcgtgcgt tggacccacc cgacccgcgg cctcgtgtcg ccgtcggact
      121 tcatccccct cgccgaggag accggcctga tcgttcccat cggccgatgg gtgatgcacg
      181 aggcggcgcg atacgcggtc gagctgcagg agcgctaccc gatgcagcca cttttccaca
      241 tggcggtcaa cctgtcggct cgccagatcg cgcggccaga gctcatcgag gaggtccgtg
      301 agatcctgct cgagaccggc ctggagccct cgacgttgat cctggagatc accgagagcg
      361 tgatgatgca ggacatggaa ctctcgatcg aacggctcgg ctccctgaag gaactgggcg
      421 tcatgctcgc cgtcgacgac ttcggcaccg gctactccag cctcaactac atccggcgct
      481 tccccgtcga catcctcaag gtcgacaagt cgttcgtgga cggcgtgagc gaaggcggcg
      541 aagcctcggc cctgaccgcc gcggtgatcg agctcgccgg catcctgaac ctgcgcccgg
      601 tcgccgaggg catcgaacga gccgaccagc tggcccgcct gctcgagctg cactgcgata
      661 tggggcaggg gttcttcttc gccaaaccac tgccgagcga agagctccag accctgctca
      721 ccgagcgggt ctcgatgcag gccgaggccg atgcactggc ccagggcatg acccagtcgt
      781 aacatcggct ccttgcgacg cctccgcccc atcctcctcg cgatcgtggt gaccaccggc
      841 gtggtcgcca ccgcgccgcc cacccttgcg gctcccccca agtgggtgca gcggatcgac
      901 gacgtggtgg gccgggacgc ggtatccgtc acgatcggct acgaggggac ggccctctac
      961 cgtcacaagg actgggtgcg gcgagccccc gcgtccaacg agaagctgct cctgtcgatg
     1021 gcactgctcg atcaggtgcc gcccgacacc acgatcccca cccgggtcct ggcgacgaag
     1081 gccgtgcgcg gcgacggcac cctgcgcggc gatctgtgga tcgtggggca cggcgatccc
     1141 gagaccgacc ggcaggacct cgcggcgctc gccgccgacc tgcgggaacg gggcgtggag
     1201 cgcgtgcgcg gtcgcgtgat gggatccacc ggtcccttcg gccgtgactg gttcgcgccg
     1261 ggatggaagt cctacttccc gaggtactac atagcgctgc cgaccgcgct cacgtatcac
     1321 tacaaccgcg gacctcgcgg taccaacatc cgcgaccccg agcgccgcgc cgccaccgag
     1381 ttgacgaagc agctgcaaca acgcggcatc aaggtgcgcg gcaaacccgg catgggcgtc
     1441 ccacccggga acctggtggg cctggccacg cggctgagtg aaccgttcgt ctcgatcatg
     1501 cggcggatga acgtgttctc cagcaacttc cgcgcagagg tgttcggcaa gttcctcggg
     1561 gcgcgggtcg gcgggcgggg cacgatcgcc cggggtgctc gcgggatcga ggcgttcacc
     1621 cgggcccacg gggtcggcgt cgaggcgaac gacggatcgg gcctgtccta cgcgaaccgc
     1681 gtcagcaccg agggcatcgt ggaactgttg tgggtggccg acgcctcacc ctggggcgag
     1741 acgctgcgcg gcacgctggc gcagggcggc cagggcacgt tggaggaccg tctgcgcgac
     1801 gtgaagatcc gcgccaagac cggcacgctc accgacatct cggcgctgtc gggatgggtc
     1861 tggctcgaac gcgaggacgc gtgggccgag ttctcgatcc tgtcgcaggg gatgagcaag
     1921 acacgttcga tccagatcga gaatgcgatc gtgcgcgtcg tgaacgcgaa cgccgcccct
     1981 cgctgatcgg gttgcgcggc ggtcagtcct cggcgcgttc gcgtggatgc agcagaccga
     2041 acgtgtaggc ctcgaccagc gcctcccacg aggcctccag gatgttctcg ctgaccccga
     2101 cggtggtcca ctcccgctcg ccgttcgacg tgtcgatcag gacgcggacc acggcgccgg
     2161 tgcccgtggt ctcgcccaac acgcggacgc ggaagtcctc caggccgatg tgcgtgagct
     2221 cgggatagtc gttcaccagt gccttgcgga gcgcatcgtc gagggccgcc accgggccgg
     2281 cgccctcggc cgactcgatg tggcgcgtgc ccttggttcg gaccttcacg gtggcctcgg
     2341 ccaggggcgg ctcgtcgtcg atcatgcgtt cctcgacgtg cacgcggtag ctctcgatct
     2401 cgaagaacgg ctgcgtccag ccgttggcgc ggcgcatcag cagctcgagc gaggcctccg
     2461 ccacgtcgaa cgcgtacccc tcagcttcgc ggtccttgat cagatcgacg gtgacgggga
     2521 tcgcggcatc ggtgagctcg aggccgactt cggccgcctt catccggatc gtggcgccgc
     2581 cgccgtagtc cgaggccacc acgccgcgac ggttgcccac ggtttcgggc tcgatgtgct
     2641 cgtaggcacg cgatacccgg gccaccccgc tggtgtgcag gccggctttg tgggtgaacg
     2701 cgtagcggcc cgaatatg
//
LOCUS       DATDBZ010000305         2736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2155207, whole genome shotgun sequence.
ACCESSION   DATDBZ010000305 DATDBZ010000000
VERSION     DATDBZ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2155207"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1787)
                     /locus_tag="VIX62_07425"
     CDS             complement(168..1787)
                     /locus_tag="VIX62_07425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5860097.1"
                     /translation="MLGVLGKYRLDQRLGGGGMAEVFLGSMLGAEGFSRKVAIKRVLP
                     GYSENEAFARMFVAEAQISSQLTHPNIVSVLDFDRDAEHRLFLVMELVEGKDLDALLG
                     SGPLPTSVIIFVIGEVLRGLGYAHDLPISNQIRGVVHRDVSPHNVLLSWAGAVKVSDF
                     GIAKAREASEATASVFIKGKPAYMSPEQANGQALDGRSDLFAVGIMLWEMLLNRRLFS
                     GGDTRSVLASVLFGQIPRPRSIRGDVPKDLERVCMKLLERDVPLRYATAEHALHDLLE
                     CSDAPKAGREQLVSLLGDRFPSEAPVRHSRMRSGNMSTPQPHLFHSQPPGTMPATPAS
                     APPAGAMPSLGAVMNAPTGTMHAPLAMTRPADGRSRVTKVAIIVVVVVMSGLITFAIA
                     MSMRGGGTKEPPVAVVPDGGGSALGVIPDTAPAPPPPAEAGPADAGPTDAPAPPADAK
                     KEAPEGQKKDPTVIVSKGLSVPFPAATVYVDGKKMGDSPGAFDVPSGKHKVRLENPDS
                     GFHNLTISVTIKVGRKVTLGFDATQKRLFLQ"
     gene            complement(1810..2319)
                     /locus_tag="VIX62_07430"
     CDS             complement(1810..2319)
                     /locus_tag="VIX62_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5860098.1"
                     /translation="MTRVRDTFCSFCGTKYPEPLSYPRACAGCGTQVWANPIPVAVVL
                     VQIVEGDRTGILVVRRNIPPQIGKLAIVGGFVEETGVIVAAETLQPMWYASSEPKPNR
                     ILMFSVAPPMAAESLPPFTTDLEASERGVVWGPGGIEDVFAFPLHIEAVRKYFGGRGI
                     TGPHDFCAR"
     gene            complement(2336..2506)
                     /locus_tag="VIX62_07435"
     CDS             complement(2336..2506)
                     /locus_tag="VIX62_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HEY5860099.1"
                     /translation="MKKTTKKLNLPRETIGLLTRQELTGIAGGMPCGTDTCPQSGRPA
                     CYATTSGFCMTG"
BASE COUNT          438 a          956 c          889 g          453 t
ORIGIN      
        1 cgagtggccg ttcttctgca gccagtgctc gatctgattc accagcgccg gttgcagcgt
       61 ggccttgccg gtcaccacga cgccaacgtc gttctggtcc tcggcgattg ccgtgccggt
      121 cgtcagcaag aacaggagga gagcgagagt tgcgcgcatc ggaagaccta ttggaggaag
      181 aggcgtttct gggtcgcgtc gaagccgagc gttaccttgc ggccaacttt gatcgtcacg
      241 ctgatcgtca ggttgtgaaa cccgctgtcg gggttctcga gtctcacctt gtgttttccg
      301 ctcgggacgt cgaaggctcc aggtgagtcg cccatcttct tgccatcgac gtagacggtc
      361 gcggccggga acggcacgga gagacccttg ctgacgatga cggtagggtc cttcttttgg
      421 ccctcgggcg cctctttttt cgcgtccgcc ggcggggccg gtgcgtcggt cggaccggcg
      481 tcggccggac ccgcttcggc aggtggaggc ggtgccggtg ccgtgtccgg gatcacgccg
      541 agcgcgctcc ctccgccgtc cggcaccacc gcgaccgggg gctccttggt gccaccgcca
      601 cgcatgctca tcgcgatcgc gaacgtgatg aggccgctca tcaccacgac cacgacgatg
      661 atcgcgacct tggtcacacg tgatcggccg tccgcaggtc gcgtcatcgc gagcggggcg
      721 tgcatcgttc cggtcggcgc gttcatcacc gcgccgaggc tcggcatcgc gccggcggga
      781 ggcgccgacg ctggggttgc cggcatcgtg cccggcggct gcgagtggaa caggtgcggc
      841 tgcggcgtgc tcatgttccc ggaccgcata cggctgtgcc gcaccggcgc ctcgctcgga
      901 aaccgatcgc cgagcagcga caccagctgc tcgcggcccg ccttcggtgc gtccgagcac
      961 tccagcagat cgtgcagcgc gtgctccgcg gtcgcgtagc ggagcggcac gtcgcgctcg
     1021 agcagcttca tgcacacgcg ctcgaggtcc ttcggcacgt ccccgcggat cgagcgcggc
     1081 cgcggaatct gcccgaacag caccgaagcc agcaccgatc gcgtgtcgcc accggagaac
     1141 agccggcggt tcaacagcat ctcccacagc atgatcccga ccgcgaacag atcgctccgc
     1201 ccgtcgagcg cctggccgtt cgcctgctcc gggctcatgt acgccggctt gcccttgatg
     1261 aacaccgagg ccgtcgcctc gctcgcctcg cgggccttcg cgatcccgaa gtccgagacc
     1321 ttcaccgccc cggcccacga cagcagcacg ttgtgcggcg acacatcccg gtgcaccacg
     1381 ccgcggatct gattcgagat cggcaggtcg tgcgcgtagc ccaggccgcg cagcacctcc
     1441 ccgatcacga agatgatcac cgacgtcggg agcggacccg atccgagcag ggcgtcgagg
     1501 tccttgccct cgaccagctc catcaccagg aacagccggt gctcggcgtc gcggtcgaag
     1561 tcgaggaccg acacgatgtt cggatgggtc agctgggacg agatctgggc ctcggcgacg
     1621 aacatccgcg cgaacgcctc gttctccgag tacccgggca gcacgcgctt gatcgcgacc
     1681 ttgcgcgaga agccctcggc cccgagcatc gagcccagaa acacttcagc catcccgccg
     1741 ccgccgaggc gttgatcgag gcggtatttc cccagaaccc ctagcatcga gcggggcgat
     1801 tataggcgat cagcgcgcgc agaagtcgtg gggcccggtg atcccacggc ccccgaaata
     1861 cttgcgcacg gcctcgatat ggagcgggaa cgcgaacacg tcctcgattc cacccggccc
     1921 ccacacgacg cctcgctccg aagcctccag atcggtcgtg aacggcggca acgactccgc
     1981 cgccatcggt ggcgcgaccg agaacatcag gatccggttc ggcttcggct cgctcgacgc
     2041 gtaccacatc ggctggagcg tctccgccgc cacgatcacc ccggtctcct cgacgaagcc
     2101 acccacgatc gcgagcttcc cgatctgcgg cgggatgttg cgccgcacga cgagaatgcc
     2161 ggtgcggtca ccctcgacga tctgcaccag caccaccgcg accgggatcg ggtttgccca
     2221 gacctgggtc ccgcagccgg cgcaggcacg cgggtacgag agcggctcgg gatacttggt
     2281 gccgcagaac gagcagaagg tgtcgcgtac gcgagtcatc gggtggagtc tactctcagc
     2341 cggtcatgca gaaaccggac gtggtcgcgt agcacgccgg tcgaccggac tgcgggcagg
     2401 tgtcggtccc gcacggcatc ccgcctgcga tcccggtgag ctcctggcgt gtgagcaggc
     2461 cgatggtctc gcgcggcagg ttcagcttct tcgttgtctt cttcactgcg ccctcctggt
     2521 tatctcgttg aggaaccgtc gatgagctgg tcgcaccagc tgcgaagatt tcagcccttg
     2581 accgatcccg ttccgcagct gagcccaccg ccgccgagac tttggacctg gcaccacggc
     2641 acaccggaca tcccccagcc tccgcgaacg gccgtgactt cgtcggctgt gaggacacgg
     2701 agcgtgtcgc gagcgagagc gagcttggtg gaatcg
//
LOCUS       DATDBZ010000306         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_313696, whole genome shotgun sequence.
ACCESSION   DATDBZ010000306 DATDBZ010000000
VERSION     DATDBZ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313696"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VIX62_07440"
     CDS             <1..1293
                     /locus_tag="VIX62_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5860100.1"
                     /translation="VPLAKAGHRVSLVSSENFMQYQPFLPEVASGTIDPRAVVVPLRP
                     ILRHVEVSVGEVIGLDHDARRATVRTADGPDVAVDYDVVVVTAGSWSRVLPVPGLAER
                     GIGFKTVQEAIYLRNHVLSRLDIAAETTDPARARSALTFVFVGAGYAGVEALGELEDL
                     ARDAIANYPTLRASQMRWVLVEAGDQILPELPPDLARYAQERLEHRRIDVLLHTKLES
                     IDGGLARLTDGKSFHADTLVWTAGVKPAPIATFSGLPVDEHGRIVVDDRLHVLGVPDA
                     WAAGDVAAVPDVTTGGISPPTAQHAMRQGKRLGENLTASILGGSQEAFVWKNVGGVCS
                     LGRYKGVADVFGVKVKGFAGWVLHRSYHLLAMPTIGRRVRIAVDWTIALLFPRDITQL
                     GGFEHPREPFRRAAGADPDGGAGPVAGADPDPDPRGDA"
     gene            1323..>2125
                     /locus_tag="VIX62_07445"
     CDS             1323..>2125
                     /locus_tag="VIX62_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY5860101.1"
                     /translation="MLATYRRDWLTKDVVAGLVLTALLVPQGMAYAELAGLPAITGLY
                     TSILCLLGYAMFGPSKILVLGPDSSLGPMIAATLAPLLVADGDPVKAVAMASVLALMV
                     GAIMILAGVAKLGFVADLLSKPTVIGYMNGLALTILVGQLPKLFGFSVDADGFIGELV
                     GFLDGVTAGDTVTAALAIGALGLILMVVLQRTLPKWPGVLVVVIASIAITAIFDLAGR
                     GVSLVGALPQGFPPFTIPQVSLSDWGTLAIGAVGIALVSLTDTISTASS"
BASE COUNT          299 a          667 c          772 g          387 t
ORIGIN      
        1 gtgccgctcg cgaaggcggg ccaccgcgtt tccttggtga gctcggagaa cttcatgcag
       61 taccagccat tcctgcccga ggtcgcgagc ggcacgatcg acccgagagc cgtggtcgtg
      121 ccgttacgtc cgatcctgcg acacgttgag gtgtcggtgg gcgaggtgat cggtctggac
      181 catgatgcgc gccgggccac cgttcgcacg gccgacggcc cggatgtggc cgtggactac
      241 gacgtcgtgg tggtcacggc gggatcgtgg tcgcgggtcc tgccggtgcc cggcctcgcg
      301 gaacgaggga tcgggttcaa gaccgtgcag gaagcgatct acctgcgcaa ccacgtgctg
      361 tcgagattgg acatcgccgc agagacaacc gacccggctc gcgcacgctc ggctttgacg
      421 ttcgtgttcg tcggcgcggg ctacgcgggc gtggaagccc tgggcgagtt ggaagacctg
      481 gctcgagacg cgatcgcgaa ctaccccacg ctgcgggctt cgcagatgcg ctgggtgctc
      541 gtcgaagcgg gggaccagat cttgcccgaa ctgccgccgg atctcgcgag gtatgcccag
      601 gagcgactcg agcatcgaag gatcgacgtg ctgctccaca ccaagctcga gtcgatcgac
      661 ggagggctgg cccggctgac cgatggcaag tccttccatg cggacaccct ggtgtggacg
      721 gcgggggtga agcccgcgcc gatcgccacc ttcagcggac tgccggtgga cgaacacggg
      781 cgcatcgtgg tggacgatcg tctgcatgtg ctcggtgtgc ccgacgcatg ggcagccggg
      841 gacgtcgccg cggtgccgga cgtcaccacc ggcggcatct cacctcccac cgcccagcac
      901 gcgatgcgcc agggcaaacg gctgggtgag aacctcacgg catcgatcct gggggggtcc
      961 caggaagcgt tcgtgtggaa gaacgtgggt ggcgtgtgct cgctcgggag gtacaagggc
     1021 gtcgcggatg tcttcggggt gaaggtgaag gggttcgccg gctgggtgct ccatcggtcg
     1081 tatcacctcc tcgcgatgcc cacgatcggt cgtcgcgtgc ggatcgccgt ggattggacg
     1141 atcgccttgt tgttcccgag ggacatcacc cagctgggcg ggttcgaaca tccgcgcgaa
     1201 ccgttccgtc gcgccgcggg cgccgacccc gacgggggcg ccgggcctgt tgcgggcgcc
     1261 gaccccgacc cggatccacg gggtgatgcg tgaaggcatc gtcggtcgtg cccggtgtca
     1321 agatgctcgc gacctatcgg cgcgactggc tgaccaagga tgtcgtcgcg gggttggtcc
     1381 tgactgcgct cctcgtgccg caggggatgg cgtacgcgga gctggcgggg ctgccggcga
     1441 tcaccgggct gtacacctcg atcctgtgtc tgctcgggta tgcgatgttc ggcccgagca
     1501 agatcttggt gctgggcccc gattcgtcac tggggccgat gatcgcggcg acgctggcgc
     1561 cgctgttggt ggcggacgga gatcccgtca aggcggtcgc gatggcgtcc gtccttgcct
     1621 tgatggtggg cgccatcatg atcctggccg gggtcgcgaa actgggattc gtggccgacc
     1681 tgttgtccaa gcccaccgtg atcgggtaca tgaacgggct cgctctcacc atcctggtcg
     1741 gccaactccc caagctcttc gggttctcgg tcgacgcaga cgggttcatc ggtgagctcg
     1801 tcgggttcct cgacggtgtc accgccgggg acacggtgac cgcggcgctg gcgatcggtg
     1861 cgctcggcct gatcctcatg gtggtcctcc agcgaacgct cccgaagtgg ccgggcgtcc
     1921 tggtggtggt gatcgcgtcg atcgcgatca ccgcgatctt cgacctggca ggtcgcgggg
     1981 tgtccctcgt cggtgcgttg ccgcagggct tcccgccgtt cacgatcccg caggtgtccc
     2041 tttcggattg gggaaccctc gcgatcgggg cggtggggat cgcgctggtg tcactgacgg
     2101 acacgatctc cacggcgtcc tcgtt
//
LOCUS       DATDBZ010000307         2775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_81965,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000307 DATDBZ010000000
VERSION     DATDBZ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81965"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VIX62_07450"
     CDS             complement(<1..846)
                     /locus_tag="VIX62_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5860102.1"
                     /translation="MPSGRGIAVLGAGIALWLTARIIGSPGMEVVGIGLTVLPFLAGA
                     FVWWGRTRIDVHRRLDDVRVPPGTRVTVQLEVENRSAAPTSFLLVEDRQPPTLGRSAR
                     LVVSGVPPRGSQRVSYTVLPQTRGRYRLGPLSVDITDPFALTRQRLEFDERDELLVTP
                     EIEDLLSGSDGAVGQSFGASRARQLFRTGEEYYTMRGYQEGDDLRRIHWPSVARTGAL
                     MIRQDESSKRASGLVFIDTRAQALGQSHSQAFERAVSVGATLGVMMARRGFSLRLGTA
                     EAPAAA"
     gene            complement(847..1764)
                     /locus_tag="VIX62_07455"
     CDS             complement(847..1764)
                     /locus_tag="VIX62_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5860103.1"
                     /translation="METNVEKVVRGKHGEIRLALVALIAEGHLLIEDVPGVGKTMLAK
                     AIARSIDCSFRRLQFTPDLLPTDVTGVNVFNQEQRDFEFRPGAIFANIVLGDEINRAS
                     PKTQSALLECMEERQVTIDTQTHPLGTPFMVIATQNPIEHEGTYPLPEAQLDRFMLRL
                     SIGYPSAEIESEILASHTSGEPLADIGPVTDAPGVAGMIEQAREVHVAPAIRRYIVDV
                     GEATRRHGDIYLGASPRAGLMIMRAARAMAAAEERDFVIPDDVKALAVPALAHRIIVT
                     ADSVMSGRTAEVVLREILSEVAVPVAEGP"
     gene            2200..2682
                     /locus_tag="VIX62_07460"
     CDS             2200..2682
                     /locus_tag="VIX62_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020550694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY5860104.1"
                     /translation="MGRALVLNATDSPLAVVPARRAVVLVIKDKAEVVLSNGMVFHSE
                     RIVMEAPSVVRLRRFVRVPYRPHAPLTRRAVFARDGWHCQYCGANAENLDHVVPRSRG
                     GQHTWENVVAACRRCNTKKEDRTPAEAGFHLARQPFAPRDGFHLTLGRLEPGWEHYLL
                     "
BASE COUNT          421 a          961 c          979 g          414 t
ORIGIN      
        1 ggcggcggcc ggcgcttctg cggtccccag gcgcagggag aagccgcgac gagccatcat
       61 gaccccgagc gtggcgccca ccgagaccgc ccgttcgaac gcctggctgt gcgattggcc
      121 cagcgcctgc gcacgggtgt cgatgaagac gagtccgctc gcgcgcttgg aggactcgtc
      181 ctggcggatc atgagggcgc cggtgcgtgc caccgatggc cagtggatcc gtcggaggtc
      241 gtcgccttcc tggtagccgc gcatcgtgta gtactcctcg cccgtgcgga acagctggcg
      301 ggcgcgggag gcgccgaagc tctgaccgac ggcgccgtcg gatccgctca ggaggtcctc
      361 gatctccggg gtcaccaaga gctcgtcgcg ctcatcgaac tcgagccgct ggcgggtcag
      421 cgcgaacggg tccgtgatgt cgacgctgag cgggcccagg cggtaccggc cccgggtctg
      481 ggggagcacg gtgtaggaga cacgttgcga gccccgtggt ggcacccccg agaccaccag
      541 ccgcgccgag cgtcccagcg tcggtggttg acggtcctcc accagcagga agctcgtggg
      601 cgcggcggaa cggttctcga cctcgagctg cacggtgacc cgggtgcccg gagggacgcg
      661 gacatcgtcg agccggcggt gcacgtcgat gcgggttcgg ccccaccaga cgaacgcgcc
      721 ggccaggaag gggaggacgg tcaggccgat ccccacgacc tccatgccgg gggaaccgat
      781 gatgcgggcg gtgagccaga gcgcgatgcc cgcgccgagc acggcgatgc cccggcccga
      841 gggcatctag ggcccctccg cgaccggaac cgccacctcc gagaggatct cgcgcagaac
      901 cacctctgcg gtccgtccgc tcatcaccga gtcggcggtg acgatgatcc ggtgggcaag
      961 ggcggggacg gccagcgcct tgacgtcgtc cgggatcacg aagtcgcgtt cctcggcggc
     1021 ggccatggca cgagcggcgc gcatgatcat gagccccgca cgagggctgg cgcccaggta
     1081 gatgtcgccg tggcggcggg tcgcctcgcc cacgtcgacg atgtagcgac ggatcgcggg
     1141 cgcgacgtgc acctcgcgcg cctgctcgat catcccggcc acgccgggag cgtcggtgac
     1201 cgggccgatg tcggcgaggg gctcgccgct ggtgtggctc gccaggatct cgctttcgat
     1261 ctcggccgag gggtagccga tcgagagccg cagcatgaac cggtccagct gcgcctcggg
     1321 gaggggatag gtgccctcgt gctcgatcgg gttctgcgtg gcgatcacca tgaacggcgt
     1381 gccgagcggg tgggtctggg tgtcgatcgt gacctggcgc tcctccatgc actcgagcag
     1441 cgccgactgc gtcttgggtg aggcgcggtt gatctcgtcg cccagcacga tgttggcgaa
     1501 gatcgcgccc ggccggaact cgaagtcgcg ctgctcctgg ttgaagacat tgaccccggt
     1561 gacatcggtg ggcagcaggt cgggggtgaa ctgcagtcgg cggaacgaac agtcgatcga
     1621 acgcgcgatc gccttggcca gcatggtctt gccgacgccg ggcacgtcct cgatcaggag
     1681 gtggccctcg gcgatcaagg cgaccagggc caaccggatc tcaccgtgct tgccccgcac
     1741 gaccttctcg acgttggtct cgatctcccg gaactgggcc gcgaaccgct gccagtcctg
     1801 gggacgctcg cgaacctcca cccggtcacc ccttcacgta cgaccggccc acgcgggccg
     1861 gcacggatcg actgcgcaaa cgacccggtc atcgtacgcc acatccacgt gagcgaaacc
     1921 cctgcaggca ccgtggtcct gcagctcagc aagggtgtag aggcggcggt ggcaagttcc
     1981 cgcaatcacg ccggccgtcg gagccgcaga ggcatggagt gagggcggcg gtccggcctt
     2041 caacgcggac gccccgcgcg gcccctattc gcaactccag gcatcacgct cggccggtcc
     2101 gccgccgctc gcgagcagat tacacccttg tgattccttc gcgccggaag gcgcccggtg
     2161 acgggatgaa ttcgcgacgg ctcccgctag acttcgtgca tgggacgtgc gctcgtcctc
     2221 aacgcgacgg attcgccgct ggcggtggtg ccagcgcggc gagcggtcgt gctcgtcatc
     2281 aaggacaaag ccgaggtcgt cctgagcaac ggcatggtgt tccactccga gcggatcgtc
     2341 atggaggccc cctcggtggt gcgtctgcgt cggttcgtgc gcgtgcccta tcggccccac
     2401 gctccgctca cccgccgggc ggtgttcgcc cgtgatggct ggcactgcca gtactgcggc
     2461 gccaacgccg agaacctcga ccacgtggtc ccgcgctcgc gggggggtca gcacacctgg
     2521 gagaacgtcg tggcggcgtg ccggcgctgc aacaccaaga aggaagaccg cacgcccgcc
     2581 gaggccggct tccatctggc gcgccagccg ttcgcacccc gggacgggtt ccacctgaca
     2641 ctcgggcggc tcgagcccgg ctgggagcac tacctcctct aagccccttg ggccggccgg
     2701 taccacccac ctcgccctca ccgatcctcg gctgctgacg gtcgtatcca ctgtgcccga
     2761 acaacagttc gggca
//
LOCUS       DATDBZ010000308         2506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1740816, whole genome shotgun sequence.
ACCESSION   DATDBZ010000308 DATDBZ010000000
VERSION     DATDBZ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740816"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VIX62_07465"
     CDS             complement(<1..709)
                     /locus_tag="VIX62_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5860105.1"
                     /translation="MALAAVLLLGVVTEVVVRGNDDLHEFGDAVLRWFEDVRTPWLTS
                     VAKVIAALTTLGAVQLIRVALAGSLVVTKRFRHLVVALATFVVSDWLVQTFLDVQRPP
                     GDVTPLIDATDFRFPSWPMTAFAITVFAMPFVLAPAGRARSRAMTAAWVVVVLVGLSR
                     LYLAADYPSNVAYAALLSWVLTETLFRWFVPDESFPVSYRSGGNAAHLDLGGARAGAV
                     KRAMADQVGLEVVEVEPF"
     gene            892..2061
                     /locus_tag="VIX62_07470"
     CDS             892..2061
                     /locus_tag="VIX62_07470"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY5860106.1"
                     /translation="MSDQPAAPADDRAPAADAAEVTKHSPARRIISGVLSLLIVGGIF
                     VFAVPKVADYSEVWKAFQSLTGLELAALIGAMVFNLFTYWWANMAALPGLKLSRAAVL
                     TQTTTSVANTLPGGGAIAVGLTYTILKSWGFTGTNVALYVGVTGIWNIFIKLALPVIS
                     IVFLVIAGESGSAYVVAAVVGVIVLAIAVGLLAALFASERLAYRIGEAVGKVMSFFLK
                     LFRKPPKTGAGDRAVQFREDTIGLVEGRWLRLTLTTILSQVALAFVLVLSLRSMGVSE
                     QDVSTAQIFAVFAFSRLLSAVPVTPGGVGVIDLGYIGGLAAAAPEAERAAVVAGVLMF
                     RALTYGVQIPLGAFTYLIWRGKKDWRRDAAEEPDEGDAATAVVDRADRPEASRGA"
     gene            complement(2009..>2506)
                     /locus_tag="VIX62_07475"
     CDS             complement(2009..>2506)
                     /locus_tag="VIX62_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5860107.1"
                     /translation="GGLVTIPLRFVVSAWLLFRRRFVAFSAFVLTWVASEALLTWLKI
                     FFHRGRPPDPLVTVNGFSFPSGHAVAGAALAVAVVLVALPPGPERRKWERVAMAFTFV
                     MALSRVYLSAHWFSDVVTGVVLGAGIAIGSAALVTEVTNVLSTRGVIKRPETPPGDPL
                     DPRLQ"
BASE COUNT          404 a          885 c          859 g          358 t
ORIGIN      
        1 cgaagggctc cacctcgacg acctcgagac cgacctggtc ggccatggcc cgcttcaccg
       61 cgccggctcg cgccccaccc aggtccaggt gcgccgcgtt gccaccgctg cggtagctca
      121 ccgggaacga ctcgtccggc acgaaccatc ggaacagcgt ttcggtgagc acccagctca
      181 ggagcgccgc atacgcgacg ttcgacgggt agtcggccgc gaggtagagg cgggacagac
      241 cgaccagcac caccacgacc caggcggccg tcatcgcccg gctgcgcgcg cggcccgccg
      301 gggcgagcac gaacggcatc gcgaacaccg tgatcgcgaa cgcggtcatc ggccacgacg
      361 ggaagcggaa gtcggtcgcg tcgatcagcg gcgtgacgtc accgggggga cgctgcacgt
      421 cgaggaaggt ctgcacgagc caatcgctga cgacgaacgt cgcgagggcg accacgaggt
      481 ggcggaaccg cttggtcacg acgaggctgc cggcgagcgc cacacggatg agctgcaccg
      541 cgcccagggt cgtgagcgct gcgatgacct tcgccacgct tgtgagccac ggcgtcctga
      601 cgtcttcgaa ccaccggagc accgcgtcgc cgaactcgtg caggtcgtcg ttgcctcgca
      661 ccacgacctc ggtcacgacc ccgagcagca gcaccgcggc cagggccaac caccatctgg
      721 ccgtgttgga tgcgtgcggg agcggagggg gttgtcccga tgggcgtcgc tgacgatgac
      781 ctcgcggcgc ggcacccggc gccggcgggc tcggcgagga ttccatcccg cggagcctag
      841 tcgaaggcga cgcgcgacgc gctcggagcc tgcacccgat atcctgcggc ggtgagcgac
      901 caacctgccg cgccggccga cgaccgggca cccgccgccg acgcagccga ggtgacgaag
      961 cactcgccgg cccggcgcat catctccggg gtcctctcgc tcctgatcgt cggcggcatc
     1021 ttcgtattcg cggtcccgaa ggtcgccgac tattccgagg tctggaaggc gttccagtcg
     1081 ctcaccgggc tcgagctcgc ggccctcatc ggcgcgatgg tgttcaacct cttcacgtac
     1141 tggtgggcga acatggccgc gctccccggg ctcaagctgt cacgggcggc ggtcctcacg
     1201 cagaccacga cctccgtcgc gaacaccttg ccgggcggcg gggcgatcgc ggtgggcctc
     1261 acgtacacga tcttgaagtc gtgggggttc accggcacca acgtggctct gtacgtgggc
     1321 gtcaccggca tctggaacat cttcatcaag ctcgccctgc cggtcatctc gatcgtcttc
     1381 ctggtgatcg cgggcgagag cggatctgcc tacgtcgtcg ccgccgtggt cggcgtgatc
     1441 gtcctcgcga tcgccgtggg gttgctcgcc gccctcttcg cgagcgaacg gctcgcgtac
     1501 cgcatcggcg aagccgtcgg caaggtgatg tcgttcttcc tgaagctgtt ccgcaagccg
     1561 ccgaagaccg gtgcgggcga tcgcgcggtg cagttccgtg aggacacgat cgggctggtc
     1621 gaaggccgct ggctccgcct cacgctcacc acgatcctga gccaggtggc cctggccttc
     1681 gtgctggtgc tgtccctgcg cagcatggga gtctccgaac aagacgtgag caccgcccag
     1741 atcttcgccg tgttcgcgtt cagccgcctg ctctccgccg tgccggtgac cccagggggc
     1801 gtcggcgtga tcgacctcgg ctatatcggt ggcctcgccg cggcggcccc ggaggccgag
     1861 cgagcggcgg tcgtggcggg agtcctgatg ttccgcgccc tgacctacgg ggtgcagatc
     1921 ccactcggcg ccttcaccta cctcatctgg cgaggcaaga aggactggcg ccgcgacgcg
     1981 gccgaggaac ctgacgaagg ggatgccgct actgcagtcg tggatcgagc ggatcgcccg
     2041 gaggcgtctc ggggcgcttg atcacccccc gggtgctcag cacgttggtc acctccgtga
     2101 cgagagcggc cgaaccgatc gcgatgccgg cgccgagcac cacgcccgtg accacatcgg
     2161 agaaccagtg cgccgacagg tagacgcgcg agagggccat cacgaacgtg aacgccatgg
     2221 cgacgcgttc ccacttccga cgctccgggc cgggggggag ggccacgagc accaccgcca
     2281 cggcgagcgc cgcccccgcc accgcgtgcc ctgaggggaa cgagaacccg ttcaccgtga
     2341 cgagtgggtc gggcggccgt ccccggtgga agaagatctt cagccaggtg agcagcgcct
     2401 ccgatgcgac ccaggtgagt acgaaggccg agaacgcgac gaaccgccgc cggaacagca
     2461 accacgccga caccacgaag cgcagcggga tcgtgacgag tcctcc
//
LOCUS       DATDBZ010000309         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2958739, whole genome shotgun sequence.
ACCESSION   DATDBZ010000309 DATDBZ010000000
VERSION     DATDBZ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2958739"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VIX62_07480"
     CDS             complement(<1..424)
                     /locus_tag="VIX62_07480"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5860108.1"
                     /translation="MSRRVPLPRATPESQGVGSVRLMGFVEALDRLEHVHSVMVVRRG
                     HVIAEGWWAPYRAERRHALFSISKSLTSTAIGLLIEDGRVSLDDRVVDLLPDDVPATF
                     DDRLASLHVRHLLSMSTGQARDGLEEAVGGDGWARRILD"
     gene            494..1021
                     /locus_tag="VIX62_07485"
     CDS             494..1021
                     /locus_tag="VIX62_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5860109.1"
                     /translation="MTRKTTASRSRRTLTEGLLDELTSWTPRDRFRMLGAWQQCGLSL
                     AHLNVVTLLEMHGPMSMGRVAELLGVSVASATGIVDRMAERYLVERRDSPTDRRVVQV
                     HIAARAHELLDAAKARRRAHLREILVRIDEPRLAGLLDGLRAVRAARAEIDQDQGHVT
                     PELVRVHTPKPAANA"
     gene            1018..>2004
                     /locus_tag="VIX62_07490"
     CDS             1018..>2004
                     /locus_tag="VIX62_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860110.1"
                     /translation="MIGLLRSYLRPYRGSIVLVLVMLLIQALATLYLPALNADIIDEG
                     IAAGDNERILEVGALMLVVTVIMAIASVFAVFWGAKVAMGFGRDLRSGVFGKVETFSQ
                     VEVARFGSPSLITRNTNDVQQVQTVVFMGLTVMVTAPIMIIGGIVMAVREDGPLALLL
                     LAVLPAMALVIGITMYRAVPLFQATQTRIDRINQVLRETLAGVRVIRAFVRTRHEEAR
                     FAVANHDVFDAGLRVNRMFAVTIPVLTAIMNLATVAVMWFGAMRINDGALQIGNLTAF
                     LQYLLQIMFSVLTAVLMFILIPRAVVSSGRIREVLETDPVIGDPPTPIRPVDT"
BASE COUNT          306 a          698 c          666 g          334 t
ORIGIN      
        1 catccaggat ccgtcgcgcc cagccgtcgc caccgaccgc ctcttccagg ccatcgcgtg
       61 cctggccggt gctcatcgac agcaggtggc gaacgtggag ggacgcgagc ctatcgtcga
      121 acgtggcggg tacgtcatcc gggagcaggt ctacgacgcg gtcgtcgagt gacacgcgtc
      181 cgtcctcgat gagcagcccg atggcggtcg aggtgagact cttgctgatc gagaacagcg
      241 cgtggcgccg ctcggcgcga tacggcgccc accagccctc ggcgatgaca tgcccccggc
      301 gcaccaccat cacgctgtgg acgtgctcga gccggtcgag ggcctcgaca aagcccatga
      361 ggcgcaccga cccgacgccc tgcgactcgg gtgtggcgcg tggaagcggc acacggcgac
      421 tcatgcgccg atgctaccgc ggaccccctc ggaagcacct gcgacattcc ttaggctgat
      481 gtagattatc aatgtgacac gtaagactac cgcgtccaga tcgcgcagga ccctcaccga
      541 gggcctgctc gacgagctca cctcgtggac gcccagggat cggttccgga tgctcggcgc
      601 gtggcaacag tgcgggctga gcctcgccca cctcaacgtg gtgacgctgc tcgagatgca
      661 cggaccgatg tccatgggcc gggtcgcgga gctgctgggc gtgtccgtcg ccagcgccac
      721 cggcatcgtg gatcgcatgg cggagcggta tctcgtggag cgcagggact ccccgaccga
      781 ccgccgggtc gtgcaggtgc atatcgccgc gcgcgctcac gagctgctcg atgccgccaa
      841 ggcccgccgc cgggctcacc tgcgggagat cctggtacgc atcgatgagc cgaggctcgc
      901 ggggctcctc gacggcctcc gagcggtgag ggccgcgcgc gccgagatcg accaggacca
      961 gggacacgtc acgcccgagc tggtccgggt gcacaccccg aagccggccg ccaacgcgtg
     1021 atcggtctcc tgcgtagcta tctgcggccc tatcggggct ccatcgtgct ggtgctcgtg
     1081 atgctgctca tccaggcgct cgcgacgctc tacctgccgg cgctcaacgc ggacatcatc
     1141 gacgagggca tcgccgcggg cgacaacgag cggatcctcg aggtcggcgc gctgatgctg
     1201 gtcgtcacgg tgatcatggc catcgcctcg gtgttcgccg tgttctgggg cgccaaggtc
     1261 gcgatgggct ttggccgaga cctccggagt ggcgtgttcg ggaaggtgga gacgttctca
     1321 caggtcgagg tcgcgcggtt cgggtcgccg tcgctcatca ctcgcaacac caacgatgtg
     1381 cagcaggtcc agaccgtggt gttcatgggc ctgaccgtca tggtcaccgc gcccatcatg
     1441 atcatcggcg gcatcgtcat ggccgtccgc gaggatggcc cgctggcatt gctgctgctg
     1501 gccgtgctgc cggcgatggc gctcgtcatc ggcatcacga tgtaccgcgc cgtgcccttg
     1561 ttccaggcca cgcagacccg gatcgaccgg atcaaccagg tgctgcgcga gacgctcgcc
     1621 ggggtccggg tcatccgcgc cttcgttcgc acccgccacg aggaggctcg gttcgcggtc
     1681 gccaaccacg acgtgttcga cgcaggcctc cgggtcaacc ggatgttcgc ggtgaccatc
     1741 ccggtgctca ccgcgatcat gaacctcgcc acggtcgcgg tgatgtggtt cggggcgatg
     1801 cgcatcaacg acggcgccct ccagatcggg aacctgaccg cgttcctgca gtacctgctc
     1861 cagatcatgt tctcggtgct caccgccgtg ctcatgttca tcctcatccc gcgggcggtc
     1921 gtgtcctccg gacgcatccg cgaggtgctc gagaccgatc cggtcatcgg cgacccgccc
     1981 acccccatcc ggcccgtgga cacc
//
LOCUS       DATDBZ010000310         5158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1947588, whole genome shotgun sequence.
ACCESSION   DATDBZ010000310 DATDBZ010000000
VERSION     DATDBZ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947588"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VIX62_07495"
     CDS             <1..324
                     /locus_tag="VIX62_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY5860111.1"
                     /translation="GNFELNVFKPVMIRNLLHSVALLADVSSTFREFCIEGLQPDRER
                     IQELVDRSLMLVTALAPSIGYDNAAAIAKSAHHEGITLKEAAVASGLLTAEEYDERVR
                     PQDMV"
     gene            425..856
                     /locus_tag="VIX62_07500"
     CDS             425..856
                     /locus_tag="VIX62_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5860112.1"
                     /translation="MTLSERDLPSPHLQRRIREVQVFLADNLDRDVDLRAIAHEASLS
                     PYYFTRQFTAYVGVPPYRYLIGLRIDRAMQLLRETELSVTQICHRVGFNSLSHFTTTF
                     RQHTGVSPSAYRRRQDWERDANRFTPITQSLVEQPNKRFTV"
     gene            complement(859..1755)
                     /locus_tag="VIX62_07505"
     CDS             complement(859..1755)
                     /locus_tag="VIX62_07505"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY5860113.1"
                     /translation="MELPDIVVTPEWLLGQLGSEPRPPDGLVIADVRWKPGASVREAF
                     AQKHLPGAVSFDVDVDLARPAFDGPGRHPLPSPEVFAHAMGAAGIGDDDLVVAYDDAG
                     GSVAARLWWMLHVTGHRAALLDLPSLETWTAIGGALATGAGSPSPEPATFTVRPWPRE
                     RIADAGQVRASLIRGDGVVVDARAGERYRGEVEPIDPVAGHIPGAVSAAWIGDRDPRT
                     DRLLTPEALRSRYAELGVRDGVSTIASCGSGVTACLAVLVLERAGLPGARLYEGSWSD
                     WTHDPSRPIVTGAEPGTMPSLR"
     gene            complement(1894..3771)
                     /locus_tag="VIX62_07510"
     CDS             complement(1894..3771)
                     /locus_tag="VIX62_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5860114.1"
                     /translation="MRSRTLGVTAALLAFALVAAACGGDSGSPTTESTAGADGEPAPG
                     GTLRIALVSDMSSAFDPQKEYYGVAWEYYRCCLLRTLMSYNGKTTAEGGGVVEPDLAS
                     ADPEVSADGLTWTFSIQPGLHYAPPVEEVEITAQDFVRAMEREATPSANVGGYPFYYS
                     VIDGFDEFASGEADEISGMKAVDDHTLEITVTRPTGDLGYRLAMPAAAPIPPNPDDPK
                     ARFGVAEGHDADYGRYLVASGPYMFQGSEDMDFSQPVSKQNPVEGYQPGRSIVLVRNP
                     SWSGDTDPLRPAYPDSIESSIGGVSEDLALKVDAGDIDLVLDGVPPSDQVQRYTTDPA
                     LKDRLHSNPSDAVRYVEMNLANPPFDDIHVRKALNYVIDKQGLLQLRGGPVFGEVAGH
                     IMVNSLQNDLLKDYDPYPSPNGAGDLTKAKEEMAQSKYDSDQDGVCDASVCEDMLTFT
                     DAAAPYPKQSALIEQNLEAIGMSMDLKALERTTMYSKCEDPNTHWQLCTGTSWGKDYA
                     DGDTFASPLFSQAAIGPQSCCNDPMVGVTDEMLQERGYDAVAVPSAEDQIDQCKPLTG
                     DERFQCWADLDTYLMEDVVPWVPFLFDNNVVVLSENVTNWSFDQFAGLPALDHLAVSS
                     S"
     gene            complement(3938..4723)
                     /locus_tag="VIX62_07515"
     CDS             complement(3938..4723)
                     /locus_tag="VIX62_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY5860115.1"
                     /translation="MKVVTWNVNSIRQRMPRLLALLTRYDPDVVCLQETKVTDEEFPF
                     DELSAAGYRATSYGQRAYNGVAIVSKEPQELVARGFTGDPTPDGARVVAVRIGDPSVA
                     SVYVVNGKAVGDPAYEIKLSWLDALRAWTTETFDLSTPVLLAGDFNVAPDDRDVHDPE
                     AWRGKNLASEPERERIRALEGAGLTDLGRAAAGDVTGPFTWWDYRMGAFHRGWGLRID
                     LMLGSGPLADHLGTVEVDREERKPSTGEGKPSDHAPVILTTSD"
     gene            4758..>5158
                     /locus_tag="VIX62_07520"
     CDS             4758..>5158
                     /locus_tag="VIX62_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY5860116.1"
                     /translation="MDQPGPSARDPSFNEANPRRDEMRSLGLRNRPWIYAFTAVAIIG
                     VVIALFWIARPTSESVPSVAGRPLSEAFAVLEDQDVCVEWIAVDRDARPGQVSGQNPQ
                     GGSEWTSDTHHVRLIVGPRTDIPAAIAEVPD"
BASE COUNT          800 a         1790 c         1708 g          860 t
ORIGIN      
        1 ggcaacttcg agctcaacgt gttcaaaccg gtgatgatcc gcaacctcct ccattcggtc
       61 gccctgctcg ccgacgtcag cagcacgttc cgcgagttct gcatcgaagg gctccagccc
      121 gatcgcgagc gcatccagga gctcgtcgat cgctcgctga tgctggtcac cgcgctcgcg
      181 ccatcgatcg gctacgacaa cgcggcggcg atcgcgaaga gcgcccacca cgagggcatc
      241 acgctcaagg aagccgccgt ggcgtccggg ttgctcaccg ccgaggagta cgacgaacgg
      301 gttcggcccc aggacatggt ctagtccatc cggaccggtc ctgaccagca ggtttgcgca
      361 ggattcctga aacccctccc tgggtaagga gcgtaatcta gggtaggagc aaagggggtc
      421 cgggttgacg ctgtctgaac gcgatcttcc cagtccgcat ctgcagagaa ggatccgcga
      481 ggtgcaggtc ttcctggcgg acaacctcga cagggacgtc gacctacgtg ccatcgcgca
      541 cgaggcgtcg ctgtccccgt actacttcac ccgccagttc acggcgtacg tgggggttcc
      601 gccgtaccgc tacctgatcg gactgcggat cgaccgggcg atgcagctgc tgcgcgagac
      661 cgagctgtcg gtcacccaga tctgccaccg cgtcggcttc aacagcctgt cgcacttcac
      721 gaccacgttc cgccaacaca cgggcgtgag tccgtcggcc tatcgccggc gccaggactg
      781 ggaacgcgat gcgaaccggt tcacgccgat cacgcagtcg ctcgtggagc agccgaacaa
      841 gcgcttcacc gtctgagatc accgcaggga cggcatcgtt ccgggttcgg cgccggtcac
      901 gatcggtctc gatggatcgt gggtccaatc cgaccaggac ccctcgtaca accgggcgcc
      961 ggggaggccg gctcgctcca acaccagcac ggccaggcat gccgtgacgc ccgatccgca
     1021 cgaggcgatc gtgctcacac cgtcgcgcac cccgagttcg gcgtaccgcg accggagcgc
     1081 ctcgggcgtc aggagccgat cggtccgtgg atcgcggtcg ccgatccacg ctgcgctgac
     1141 cgccccgggg atgtggccgg ccaccgggtc gatcggttcg acctcgcctc ggtagcgctc
     1201 ccccgccctg gcgtccacca cgacgccgtc gccgcggatc agcgacgccc gaacctggcc
     1261 ggcgtccgcg atccgttccc gtggccaggg tcgcacagtg aacgtggcgg gctccggcga
     1321 aggcgagccc gcgccggttg ccaacgcccc gccgatcgcc gtccacgtct cgagcgaggg
     1381 caggtcgagc aacgcagcgc gatggccggt gacgtgcaac atccaccaca acctggccgc
     1441 gacggatccc ccggcgtcgt cgtaggcgac cacgaggtcg tcgtcgccga tgcccgctgc
     1501 gcccatcgcg tgcgcgaaca cttcgggcga gggcaatgga tggcggcccg gaccgtcgaa
     1561 cgccggtcgg gcgaggtcga cgtcgacatc gaacgagacc gctccgggca ggtgtttctg
     1621 agcgaacgcc tcgcggaccg aggcccccgg cttccatcgc acgtcggcga tcacgaggcc
     1681 gtcggggggc cggggctccg atccgagctg gcccaggagc cactcgggcg tcacgacgat
     1741 gtcggggagc tccacggacg agaagggtac ccggaccgct cctgcagggg gtccatcacg
     1801 caggagcggc ccgggcctcg cggcgggggt ctcccggaac ccgaccctcg tcgccgggtt
     1861 ccggaacgtc acgtcatcgg gttcgcacgt tgactacgac gatgagaccg cgaggtgatc
     1921 gagtgccggg agcccggcga actgatcgaa cgaccagttc gtgacgttct ccgacagcac
     1981 gaccacgttg ttgtcgaata ggaagggcac ccagggcacc acgtcctcca tcaggtaggt
     2041 gtccaggtcg gcccagcact ggaagcgctc gtcgccggtg agcggtttgc actggtcgat
     2101 ctggtcctcg gcgctcggca ccgcgaccgc gtcgtagccc cgctcctgga gcatctcgtc
     2161 ggtcacgccg accatcgggt cgttgcagca tgactgcggc ccgatcgctg cctgggagaa
     2221 cagcggcgag gcgaacgtgt cgccatcggc gtagtccttg ccccacgacg tccccgtgca
     2281 gagttgccaa tgggtgttgg ggtcctcgca cttggagtac atcgtggtgc gctcgagcgc
     2341 cttgaggtcc atcgacatcc cgatggcctc caggttctgc tcgatcaacg ccgactgctt
     2401 cggatacggc gcggcagcgt ccgtgaaggt caacatgtcc tcgcacacgc tggcgtcgca
     2461 cacaccgtcc tggtcactgt cgtacttcga ctgcgccatc tcctccttcg ctttcgtgag
     2521 gtcgccggca ccgttcggtg acgggtaggg gtcgtagtcc ttcagcaggt cgttctggag
     2581 gctgttgacc atgatgtgac cggccacctc gccgaacacc gggcctccac ggagctgcag
     2641 gagtccctgt ttgtcgatca cgtagttgag cgccttgcgt acgtggatgt catcgaacgg
     2701 cgggttggcc aggttcatct ccacgtagcg gacggcgtcg gacgggttcg agtgcagtcg
     2761 gtccttcaac gcggggtccg tcgtgtaccg ctgcacctgg tccgacggcg gaaccccgtc
     2821 gagcaccagg tcgatgtcgc cggcgtccac cttcagcgcg aggtcctccg acacgccgcc
     2881 gatcgaggat tcgatcgaat cgggataggc gggacggagc gggtcggtgt ccccggacca
     2941 ggaggggttg cggaccagca cgatggaacg cccgggctgg tagccctcga ccgggttctg
     3001 cttcgacacc ggctgggaga agtccatgtc ctccgagccc tggaacatgt acggacccga
     3061 ggccaccagg tagcggccgt aatccgcgtc gtgtccctcg gcgacgccga agcgcgcctt
     3121 ggggtcgtcg gggttcggcg ggatcggcgc cgcggccggc atcgccagcc ggtacccgag
     3181 gtcgccggtc ggacgcgtca ccgtgatctc caaggtgtga tcgtccacgg ccttcatgcc
     3241 gctgatctcg tcggcctcac cggacgcgaa ctcgtcgaat ccgtcgatca ccgagtagta
     3301 gaaggggtac ccaccgacgt tggccgacgg ggtggcctcg cgttccatcg cgcgcacgaa
     3361 gtcctgcgcc gtgatctcga cctcctcgac cggcggggcg taatgcagac ccggctggat
     3421 cgagaacgtc cacgtgagcc cgtcggccga gacctccggg tcggcggacg cgaggtcggg
     3481 ctccacgaca ccgcctccct cggcggtcgt cttgccgttg tagctcatca gcgtccgcaa
     3541 caagcaacag cggtagtact cccacgcgac gccgtagtac tccttctgcg gatcgaacgc
     3601 gctcgacatg tccgagacga gcgcgatcct caacgtccca ccgggcgcgg gttcgccatc
     3661 cgcgcccgcc gtgctctcgg tcgtgggcga gcccgaatcg ccgccgcacg ctgccgccac
     3721 gagcgcgaac gcgagcaacg cggcggtcac ccccaaggtt ctgctgcgca tggcactccc
     3781 ctctcgccgg ccacggtcgt ggccctttcc cggtgctacg ggtgatgatg gaggggccgg
     3841 gttccggaag gttgcctttg ggttgtgcct tcctgcccaa gggttccgac ttcgtgccct
     3901 ggcttgcggg gtcttgccgt tcggaggttt cgcagcgtca atccgaggtg gtgaggatca
     3961 ccggcgcgtg gtcgctgggc ttgccttcgc ccgtgctcgg cttgcgctcc tcacggtcca
     4021 cctcgaccgt cccgaggtgg tcggcgagcg ggcccgatcc cagcatcagg tcgatccgca
     4081 gtccccatcc acgatggaac gcccccatgc ggtagtccca ccacgtgaac ggtccggtga
     4141 cgtcaccggc tgccgcgcga ccgagatcgg tgagcccggc tccctcgagc gctcggatcc
     4201 gctcacgctc cggttcgctt gccaggttct tgccccgcca ggcctccgga tcgtggacgt
     4261 cgcggtcgtc gggcgcgacg ttgaagtcgc ctgccaggag cacgggcgtc gacagatcga
     4321 acgtctccgt cgtccatgca cggagcgcgt cgagccacga caacttgatc tcgtacgcgg
     4381 ggtcgccgac cgccttgccg ttcaccacgt acacgctggc gacgctgggg tccccgatcc
     4441 ggacggccac gacccgcgct ccgtccggcg tcgggtcgcc cgtgaagccg cgggccacga
     4501 gctcctgcgg ttccttggac acgatcgcga cgccgttgta ggcgcgctgg ccgtagctgg
     4561 tggcccggta gcccgcggcc gagagctcgt cgaaggggaa ttcctcgtcg gtcactttcg
     4621 tctcctgcag gcacacgaca tcggggtcat atcgcgtgag cagcgcgagc agccggggca
     4681 tccgctggcg gatcgaattc acgttccagg tgacgacctt cacgccgcgg agcatagcgg
     4741 cggtacgctc atcggcgatg gatcaaccgg gcccgtcggc gcgtgaccct tccttcaatg
     4801 aggcgaatcc gcgccgcgac gagatgcggt ccctgggctt gcgcaaccgc ccatggatct
     4861 acgcgttcac ggccgtcgcg atcatcggcg tcgtcatcgc gctcttctgg atcgccaggc
     4921 cgacatccga atccgttccg agcgtcgccg gtcgtccgct cagcgaagcc ttcgccgtgc
     4981 tggaggacca agatgtctgc gtggagtgga tcgccgtcga ccgcgatgcg cggccgggac
     5041 aggtgagcgg gcagaacccc cagggcggat ccgagtggac gagcgacacc caccatgttc
     5101 gcttgatcgt aggtccgcgc accgacatcc cagccgcgat cgccgaggtg cccgacat
//
LOCUS       DATDBZ010000311         4653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1111460, whole genome shotgun sequence.
ACCESSION   DATDBZ010000311 DATDBZ010000000
VERSION     DATDBZ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111460"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VIX62_07525"
     CDS             <1..1171
                     /locus_tag="VIX62_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5860117.1"
                     /translation="TVTIEDVDLARLRDERLVRLQEQMRAHDLDVLLLANEPNIRYAT
                     GATAMPPYAMSTFVRCAVVPVEGIPIMFEHGNSMHRSSGIALDVRRMHAWEFFDDPMS
                     EAQVWAGEVVAAMDQLGVDRSRIGLDRMGTPGFLALTEAGCGFVDSAPVTQTAREVKT
                     PEEIRLFRANAPTVMEQLRTVESTIGPGVQEREIFAAMARTGLARGVEYYATNTVCSG
                     PNTNPWRAEATDRAIEPGDLVYVDTDTVGVGGAFFCVSRTFACGPPSPAQRDTYRAAF
                     DWLERMKALIRPGITCGQLAEIAPLPPQRYLPQRYECMIHGVGLEEENPSVCFPADGQ
                     SNGDRVLEPGMLLVIELYCGEVGADHGVKLGDEVLVTNEGIEVLAPYPFDDALRS"
     gene            1266..2177
                     /locus_tag="VIX62_07530"
     CDS             1266..2177
                     /locus_tag="VIX62_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY5860118.1"
                     /translation="MPRSMWKGAISFGLVTIPVAVYPATEEKTLKFNQLHDEDMGRIR
                     YKRMCEKDGEIVDFDHIVKGYEYDKDRYVVLTDEDFDKVPVESSRAIDIQQFVDLEEI
                     DPMMYKKSYYLIPEETGAKAYALLREAMSQDNKVGIAKVSFRDKEHLAALRFSGDAFV
                     LETMYWPDELREADFGGTKVDHKVRGQELEMARQLIANLTADWNPDEYTDDYREAMLT
                     IVEAKINGEEIETVEAEPTAKVVDLMEALKASVAAAKKRADDGEDEAPARPARKKTAA
                     KKSSAKKSTAKKSTAKKTTARKKAVGE"
     gene            2174..3103
                     /locus_tag="VIX62_07535"
     CDS             2174..3103
                     /locus_tag="VIX62_07535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860119.1"
                     /translation="MIEGLPAGVRARPATRADLPAVVDLFLTYDATLQPEPDPLNEYL
                     QWIWGLPYVDLARDTVILLDDDGVVGFAQGISDPETGGPFSVDWGVALAYDLDAVGTP
                     LLTWCERQRDDREIREPIRTSIVAEDDRARALLEGRGYVQVRLMWDMVRSLGEPQDLD
                     VDPDGVEVRSFRVGQDEQLLYRIDETAFRDHWDHVDRTYEAFAARMFGESWDPSLAFL
                     AEVDGEPAGELVAIADVGRGWIASVGVLRAYRGRGAAKALLRRAFAELGARGQERVEL
                     SVDATNPTGAVALYEGIGMHVARSYAIFDGPAG"
     gene            3153..3491
                     /locus_tag="VIX62_07540"
     CDS             3153..3491
                     /locus_tag="VIX62_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY5860120.1"
                     /translation="MKLVTAIVKPHRIDEVKETLRELGVNGLTTTDVEGFGRQRGHTE
                     VYRGAEYQVDFVPKVKLEVLVPDGEVQEVVDAIVKAARTGKIGDGKLWVTEAEQVIRI
                     RTGEMGPDAV"
     gene            3514..>4653
                     /locus_tag="VIX62_07545"
     CDS             3514..>4653
                     /locus_tag="VIX62_07545"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860121.1"
                     /translation="MNATEPVTRLRRELGALDRAYSEGHHGLWSAARRSDLFDAALRE
                     LFEAPGAPAGVALTAIGGYGRRQQLPRSDVDLLVLHEGDRLDEVAALVERLLYPLWDA
                     GFEVGHAVRTAEECVVAARERLDGLTSMLDLRHLAGDRGLADRAGASVRAIAADDPDG
                     FAEVLRADARVRNERVGPAASMLQPDLKSGAGGLRDLQSLRWFEAVSGVGLEGAGLLR
                     PQERTALEDAEEFLTRVRSALHLETGKRADVLPLELQPPIARAMGFTDEPRLLAEDGL
                     MRAVFEHARAVRWITGNVWDRISAGVAPRPVPPIDGPAGVLEALADAAETDAPPSPAL
                     LDAIENVDLAAEVEWTEEVRGAFLRLLRRGAAGVSAFEALDRLGLL"
BASE COUNT          754 a         1531 c         1674 g          694 t
ORIGIN      
        1 caccgtgacg atcgaggatg tggacctcgc gcgtctgcgc gacgagcggc tcgtgcggct
       61 ccaggagcag atgcgcgccc atgacctgga tgtgctgctc ctcgcgaacg agccgaacat
      121 ccgctatgcg accggcgcca cggcgatgcc gccgtacgcg atgagcacgt tcgtccggtg
      181 cgcggtggtc cccgtggaag ggatcccgat aatgttcgaa cacgggaact cgatgcatcg
      241 ctccagcggg atcgcgctgg acgttcgccg gatgcacgcg tgggagttct tcgacgatcc
      301 gatgtccgag gcgcaggtgt gggcgggcga ggtcgtggcg gcgatggatc agctcggcgt
      361 ggaccgttcg cggatcgggc tcgaccgcat ggggacaccc gggttcctcg cgttgacgga
      421 ggcgggatgc gggttcgtgg attcggcgcc ggtcacgcag accgcccgcg aggtcaagac
      481 gcccgaggag atccgtctgt tccgggccaa cgcgccgacc gtgatggagc agctccgcac
      541 ggtggagtcg acgatcggac cgggggtgca ggagcgcgag atcttcgcgg ccatggcgcg
      601 gaccgggctg gcccgggggg tcgagtacta cgccacgaac acggtgtgct caggccccaa
      661 cacgaacccg tggcgggccg aagccacgga ccgcgcgatc gagcccggcg acctcgtcta
      721 cgtcgacacc gacacggtag gcgtgggcgg ggcattcttc tgcgtctctc gaaccttcgc
      781 gtgcgggccg ccctcgcccg cgcagcgcga cacctaccgc gccgcgttcg actggctcga
      841 acggatgaag gcgctcatcc ggccggggat cacctgtggg cagctcgctg agatcgctcc
      901 gctcccgccc cagcggtacc tgccgcaacg gtacgagtgc atgatccacg gggtcgggct
      961 cgaggaggag aaccccagcg tctgcttccc ggccgacggg cagtccaacg gcgaccgcgt
     1021 cctggagccg ggcatgctcc tggtgatcga gctctattgc ggggaggtgg gcgccgacca
     1081 cggcgtgaaa ctcggcgacg aggtcctggt cacgaacgag gggatcgagg tcctcgcccc
     1141 gtaccccttc gacgacgctc tgcgctcctg aatcctgccc cttccggcgt gtccggggcc
     1201 gcgactagac tcggatggtt cgcccgcacc cgcggcgggt aaccaccgaa ccggagggat
     1261 acgacatgcc cagatcgatg tggaaagggg ccatctcgtt cggcctcgtg acgatcccgg
     1321 tggccgtgta tccggccacc gaggagaaga ccctgaagtt caaccagctc cacgacgagg
     1381 acatggggcg catccgctac aagcggatgt gcgagaagga cggcgagatc gtcgacttcg
     1441 accacatcgt caaggggtat gagtacgaca aggaccgcta cgtcgtgctc accgacgagg
     1501 acttcgacaa ggtgccggtc gaatccagcc gcgccatcga catccagcag ttcgtcgacc
     1561 tcgaagagat cgaccccatg atgtacaaga agtcctacta cctgatcccc gaggagaccg
     1621 gcgccaaggc gtacgcgctc cttcgcgagg cgatgagcca ggacaacaag gtgggtatcg
     1681 ccaaggtcag tttccgcgac aaggagcacc tggccgcgtt gcggttctca ggcgatgcgt
     1741 tcgtgctgga gacgatgtac tggcccgacg agctgcgcga ggccgacttc ggcggcacga
     1801 aggtcgatca caaggtccgt gggcaggagc tcgagatggc gcgccagctg atcgccaacc
     1861 tcaccgccga ttggaacccc gacgagtaca ccgacgacta ccgcgaggcc atgctgacga
     1921 tcgtcgaggc caagatcaac ggcgaggaga tcgagacggt cgaggccgag cccaccgcca
     1981 aggtcgtcga cctcatggag gcgctgaagg ccagcgtcgc ggcggccaag aagcgcgccg
     2041 acgacgggga ggacgaggct cccgcgcgac cggcccggaa gaagaccgcc gcgaagaagt
     2101 cctcggccaa gaaatccacg gcgaagaagt cgaccgcgaa gaagacgacg gcacgcaaga
     2161 aggccgtcgg cgagtgatcg aggggttgcc ggcgggcgtc cgcgctcgtc cggccacccg
     2221 ggctgacctc ccggcggtcg tcgacctgtt cctcacctac gacgccacgc tgcagcccga
     2281 gcccgatccg ctcaacgagt acctccagtg gatctggggg ctgccctacg tcgacctggc
     2341 acgcgacacc gtgatcttgc tggacgacga cggggtcgtc ggtttcgccc agggcatctc
     2401 ggaccccgag acgggcggcc cgttctcggt ggactggggg gtcgcgcttg cctacgatct
     2461 cgatgcggtc gggacgccgc tcctcacgtg gtgcgaacgc cagcgcgacg atcgcgagat
     2521 ccgcgagccg atccgcacgt cgatcgtggc cgaggacgac cgagctcggg cgttgctcga
     2581 gggccgtggt tacgtccagg ttcgcttgat gtgggacatg gtgcgctcac tgggcgagcc
     2641 tcaggacctc gacgtcgacc ccgacggcgt ggaggtcagg tccttccggg tgggtcagga
     2701 cgaacaactc ctctaccgga tcgacgaaac cgcgttccgc gatcactggg atcacgtgga
     2761 tcgcacctac gaggcgttcg ccgccaggat gttcggggag tcgtgggatc cgtccctggc
     2821 cttcctcgcc gaggtcgatg gtgagcccgc gggcgaactc gtcgcgatcg cggacgtggg
     2881 gcgcggctgg atcgcctcgg tcggggtcct ccgcgcgtac cggggccgag gtgccgcgaa
     2941 ggccctgttg cgtcgtgcct tcgccgagct cggtgctcgc ggccaggagc gggtggaact
     3001 ctcggtcgat gccaccaacc ccaccggtgc tgtggcgctg tacgagggca tagggatgca
     3061 cgtcgctcgc agctacgcca tcttcgacgg gcccgcgggc taggcttacg cccggcctgc
     3121 cgagcatcgg caggacctat caggaggaac ccgtgaagct cgtcaccgcc atcgtgaagc
     3181 cgcaccgcat cgacgaggtg aaggaaacgt tgcgcgagtt gggcgtgaac gggctcacca
     3241 cgaccgacgt cgaagggttc ggccgtcagc gcggccacac cgaggtctat cgcggcgccg
     3301 agtaccaggt cgacttcgtg cccaaggtca agctcgaggt gctggtcccc gacggcgagg
     3361 tccaggaggt cgtcgacgcc atcgtgaagg cggcccgaac gggcaagatc ggcgacggga
     3421 agctgtgggt caccgaggcg gagcaggtca tccggatccg caccggcgag atgggtcccg
     3481 acgccgtctg aggatggctc cgtcgagacg gccatgaacg ccaccgaacc ggtcacccgt
     3541 ctgcgccgtg agctgggcgc cctcgatcgc gcctactccg agggccatca tggcctctgg
     3601 tcggcggcca ggcgaagcga tctgttcgac gccgcgctcc gggaactgtt cgaggccccg
     3661 ggagccccgg cgggggtcgc gctcaccgcg atcggtggct acggccggcg ccagcagctg
     3721 ccgcgttccg acgtggacct gctggtgctg catgagggcg accggctcga tgaggtcgcg
     3781 gccctggtcg aacgcttgct ctatccgctg tgggacgccg ggttcgaggt ggggcacgcg
     3841 gtgcgcaccg ccgaggagtg cgtcgtggct gcccgtgagc gcctcgacgg gctcacctcg
     3901 atgctcgacc tgcggcacct ggcgggggac cgggggctcg ccgatcgggc gggcgcgtcg
     3961 gtgcgggcga tcgcggcgga cgaccccgat gggttcgccg aggtgctgcg cgccgacgcc
     4021 cgagtacgca acgaacgcgt gggcccagcc gcctccatgc tgcagcccga tctgaaatcg
     4081 ggtgcgggcg gcctccgcga cctgcagtcg ttgcggtggt tcgaagccgt cagcggcgtc
     4141 ggtctggagg gcgcaggcct gctgcgtccg caggaacgga cggcgctgga ggacgccgag
     4201 gagttcctca ccagggtccg aagtgcgctc catctcgaga ccggcaagcg ggccgatgtc
     4261 cttccgctcg agctgcagcc accgatcgcc agagcgatgg gcttcaccga cgaacccagg
     4321 ctcctggccg aggacgggct gatgcgggcg gtgttcgaac acgctcgcgc cgtccgatgg
     4381 atcacgggca acgtgtggga ccgcatttcc gcaggtgtgg cgccgcggcc ggtgccgccg
     4441 atcgatgggc ccgcgggggt gctcgaagcc ctcgccgacg ccgccgagac cgatgcgccg
     4501 ccgtccccgg cgctgctcga tgcgatcgag aacgtcgatc tggcggccga ggtcgagtgg
     4561 accgaggagg tccgcggagc cttcctgcgg ctgctccgcc gtggcgcggc cggcgtgtcg
     4621 gcgttcgagg cgctcgaccg tctcggtctg ctg
//
LOCUS       DATDBZ010000312         4095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2559415, whole genome shotgun sequence.
ACCESSION   DATDBZ010000312 DATDBZ010000000
VERSION     DATDBZ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2559415"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VIX62_07550"
     CDS             <1..890
                     /locus_tag="VIX62_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5860122.1"
                     /translation="EVIRRLAMGPPVARLGRLAGWDPVPLTVREARRMGAALIGDAGR
                     PGAQVAAPGPTLVELRGVEVRHGDVPALRDIDLTIGRGEIVAVMGRNGAGKTTLLRAI
                     AGVHEPFRGHVTVDGHIPQPGVDTGMCPQEPESILFAETVADEVRTTLKARGLGTGPE
                     PVLEALGIEDLAERHPRDLSAGQRLLVATAAVAAGLAPAILLDEPTRGLDPDAKDRLV
                     RYLRRHAADGGVAMLATHDVELAAAVATRVVMLAGGEVIADGDPGAVLGDSTVFAPQM
                     TRAFGPGWLTPEQVAEALA"
     gene            887..1780
                     /locus_tag="VIX62_07555"
     CDS             887..1780
                     /locus_tag="VIX62_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEY5860123.1"
                     /translation="MIDVERTEVAGAPEAGVAVPSAGAPEAGVAVPSAGARELPRARY
                     RERSRIRIAVLIAVNLIGLMAFLWPFLLPLAPGVNENHRIDGPLILCVLLLCLGSVLF
                     VELGRGGLGPKAVALLGVLGAAMVALRLPGFVFGFSAMFIVVLVAGNAFGPGFGFLLG
                     MVGTFASGLFLGGLGPWLPFQMVAVGWVGMGAGLLPKGSWRVRIGSLAAYGAVSGYLF
                     GAIMNLWSWPFLTTGSAISWDAGASAATNLRHYLTYYAATSLAWDTFRAVGNAVLVLV
                     LGRPLLGALDRAARRMRLDVG"
     gene            1851..2063
                     /locus_tag="VIX62_07560"
     CDS             1851..2063
                     /locus_tag="VIX62_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860124.1"
                     /translation="MWEDPKAFPTPFGEEQEREHRYHEDHGDHEHGEGCGHDAVEHGD
                     HVDYVHDGHRHWWHHEHGHWHEHDGD"
     gene            2250..2921
                     /locus_tag="VIX62_07565"
     CDS             2250..2921
                     /locus_tag="VIX62_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5860125.1"
                     /translation="MSTEAPTPRTRVRRLPELGVYERDALNQILDEALICYVAYTAED
                     GDPRVIPTIHARIGDTLYFHGSRASQTLRALRDGANLAVTATIIDGIRFARSMFEHSM
                     NYRSVVAYGSPTEVTDLDELRTVFRAITNHVAPGRGEDARMPTDDELRQTLFLKIPLD
                     EASAKVSSGQATDIDDDYALDIWAGIMPLHTVPGEPISDPLLKAGVPTPDYITGWKRP
                     GASDA"
     gene            2963..>4095
                     /locus_tag="VIX62_07570"
     CDS             2963..>4095
                     /locus_tag="VIX62_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5860126.1"
                     /translation="MPREAWILFVGAFVNRLGTFVLPFITLYLTDRGYTVPQAGLGLA
                     GYGLGAIGAQGVGGLLADRLGRRNTIAVSMFGGSLLTLSLVWVSGLWAIIGVVILLGF
                     VAELYRPASSALIVDLIPSERRVAAFSTYRLMLNLGFAVGLALGGVLATRSYDLLFIG
                     DAITAASFGAIALVALPHGTRTTKHDERHLEGTGRIMARDTGFLLFLGAVFLTASVYM
                     QNATTFALHVKDLGYSPRTYGFLQAGNGALVVLFELPVTAWTQHRNRTHMVALGAALV
                     GLGFATLAVVEAVPGLILMVIIWTLGEIVESPATAAFVGDRAPDHARGRYQGALGAMY
                     GFAAVAGPLLGTSIYHVSPTALWWGCGLVGLMSAGLALRAGHFP"
BASE COUNT          574 a         1451 c         1430 g          640 t
ORIGIN      
        1 cggaggtgat ccgccggttg gcgatgggcc cgccggtcgc gcgcctcgga cggctggccg
       61 gctgggaccc ggtgccgctc acggtgcggg aggcgcgccg gatgggagcc gcgctgatcg
      121 gagacgccgg tcgcccgggg gctcaggtcg ccgcacccgg ccccaccctc gtcgaactgc
      181 gaggcgtcga ggtgcggcat ggcgacgtgc ccgccttgcg cgacatcgat ctcacgatcg
      241 gccgcggcga gatcgtcgcc gtgatgggcc gcaacggcgc cggcaagacc acgctgttgc
      301 gcgcgatcgc cggcgtgcac gaaccgttcc gtggccacgt gacggtggac ggccatatac
      361 cccaacccgg tgtcgacacc gggatgtgtc cgcaggagcc cgagtcgatc ctgttcgccg
      421 agacggtggc cgacgaggtt cgcaccacgt tgaaggctcg cggcctgggg accggacccg
      481 agccggtgct cgaggccctg ggcatcgagg acctcgccga gcgtcatcca cgagacctga
      541 gcgccgggca acggctgctg gtggcgacgg ccgccgtggc ggccggcctg gcccccgcga
      601 tcctgctcga cgaacccacg cgcggactcg accccgatgc gaaggaccgg ctggtgcgct
      661 acctgcgccg gcacgcggcc gacggcggcg tggcgatgct cgccacccac gacgtggaac
      721 tcgcggcggc ggtggccacc cgcgtggtca tgctcgcggg cggcgaggtg atcgccgacg
      781 gcgaccccgg cgcggtgctg ggcgattcga cggtgttcgc cccgcagatg acgcgcgcgt
      841 tcggtcccgg ttggctcacg ccggaacagg tcgcggaggc gctggcgtga tcgacgtgga
      901 gcggacggaa gtagcggggg cgccggaggc cggggtagcg gtgccgagcg cgggggcgcc
      961 ggaggccggg gtagcggtgc cgagcgcggg cgcgcgggaa ctgccgcgcg cgcggtaccg
     1021 cgaacgatcg cgaatccgca tcgccgtgct catcgccgtc aacctgatcg ggctcatggc
     1081 cttcctgtgg ccgttcctgc tgccgctcgc ccccggggtc aacgagaacc accggatcga
     1141 cggtcccctg atcctgtgcg tgctcctgct gtgcctcggt tcggtgctct tcgtcgaact
     1201 cggccgcggc ggactcgggc cgaaggcggt cgcgctgctc ggcgtcctgg gtgcggcgat
     1261 ggtggcgctc cgactccccg ggttcgtgtt cgggttcagt gcgatgttca tcgtggtcct
     1321 ggtggcgggc aacgcgttcg gacccgggtt cgggttcctg ctggggatgg tgggaacgtt
     1381 cgcgagcggt ctgttcctgg gtgggctggg cccgtggctg ccgttccaga tggtggcggt
     1441 gggctgggtc gggatgggcg cgggcctgct gccgaagggc tcgtggaggg tgcgcatcgg
     1501 ctcgctcgcg gcgtacggag ccgtcagcgg ctacctcttc ggggcgatca tgaacctgtg
     1561 gtcctggccg ttcctcacca cgggttcggc gatcagctgg gacgccggcg cgagcgccgc
     1621 caccaacctg cgccactacc tgacctacta cgcggcaacc tcgctggcgt gggacacgtt
     1681 ccgagccgtg ggcaacgcgg tgctggtcct ggtgctgggc cgtccgctcc tgggggccct
     1741 cgaccgcgcg gcgcggcgga tgcgcctcga cgtgggatga ggtaagaaag cctcgaaccc
     1801 gttcactccg tcaggaagga tcgtcatcga tgtcgagcat cccgccacag atgtgggaag
     1861 accccaaggc gttcccgacg ccgttcggcg aggagcagga gcgcgaacac cgctaccacg
     1921 aggaccacgg cgaccacgag cacggcgaag gatgcggcca cgacgcggtc gagcacggtg
     1981 accacgtcga ctacgtgcac gacggacatc gccactggtg gcatcacgag cacggccact
     2041 ggcacgagca cgacggcgac tgacccgccg gcgccgcgcg cggatgtcct gcatccctcc
     2101 cgccgtcgat cgtcaacggg tataccgatc gcgtcctatg acggaacccg tacgccgttg
     2161 acgatcgact gagcgaccac ccccgcgcat atcaccatcc ggccacgagc aaggcctgca
     2221 cccccacctc ccgggtagca tctggggcca tgtcgaccga ggcacccacc ccgcgaaccc
     2281 gcgtccgccg cctgcccgag ctgggcgtct acgaacgcga cgcgctcaac cagatcctcg
     2341 acgaagccct gatctgctac gtcgcctaca ccgccgaaga cggcgacccc cgcgtgatcc
     2401 ccacgatcca cgcgcgtatc ggcgacaccc tctacttcca cggctcgcgc gcctcgcaga
     2461 ccctgcgcgc cctgcgggac ggcgccaacc tcgccgtgac cgccacgatc atcgacggca
     2521 tccggttcgc ccgctcgatg ttcgaacact ccatgaacta ccgctccgtc gtggcctacg
     2581 gctcccccac cgaggtgacc gacctcgatg aacttcgcac ggtcttcagg gcgatcacga
     2641 accacgtcgc tcccggccgc ggcgaggatg cccggatgcc caccgacgac gagctgcgcc
     2701 agacgctctt cctgaagatc ccgctcgacg aggcctccgc gaaggtcagc tccggccagg
     2761 cgaccgacat cgacgacgat tacgccctcg acatctgggc cggcatcatg cccttgcaca
     2821 ccgttcccgg cgagccgatc agcgacccgc tgctgaaagc tggcgtacct acgccggact
     2881 acatcaccgg ctggaagcgc cccggcgcct ccgacgcgtg agcgagtcgg ccagacccac
     2941 cctccgccag aacctccgag gcatgcctcg tgaggcatgg atcctgttcg tcggcgcgtt
     3001 cgtcaaccgg ctcggcacgt tcgtcctgcc gttcatcacg ctgtacctga ccgatcgcgg
     3061 ttacaccgtg ccgcaggcag ggttggggct cgcgggctac ggcctcggcg cgatcggcgc
     3121 ccagggcgtc ggtggcctgc tggcggaccg gctgggacgc cgcaacacga tcgccgtctc
     3181 gatgttcggc gggtcgctgc tgacgctctc gctcgtctgg gtctccggtc tgtgggccat
     3241 catcggcgtc gtcatcctgt tggggttcgt cgccgagctc taccggccgg cttcgagcgc
     3301 cctgatcgtc gatctgatcc cgtccgagcg ccgggtcgcc gccttcagca cgtaccggtt
     3361 gatgctgaac ctggggttcg cggtgggcct cgccctcggc ggcgtgttgg cgacccggtc
     3421 ctacgacctg ttgttcatcg gcgacgccat caccgccgcc tcgttcgggg cgatcgcact
     3481 ggtggcgctg ccgcacggca cccggaccac gaagcacgac gagcggcatc tcgagggcac
     3541 cggccggatc atggcgcgag acaccggatt cctgctcttc ctcggcgcgg tgttcctgac
     3601 cgcatcggtg tacatgcaga acgccaccac gttcgcgctc cacgtgaagg acctgggcta
     3661 ttcgccacgg acctacggct tcctgcaggc cggcaacggc gcgttggtcg tgctgttcga
     3721 attgcccgtc accgcatgga cgcagcatcg caaccgcacg cacatggtgg cgctgggcgc
     3781 ggcgctggtg gggctgggct tcgccacgct cgcagtggtc gaggcggtgc cgggactgat
     3841 cctgatggtg atcatctgga cgctgggcga gatcgtggaa tcgccggcca ccgcggcgtt
     3901 cgtgggcgac cgagcgccgg accatgcgcg aggtcgctac cagggcgcgc tgggagccat
     3961 gtacggattc gccgcggtcg caggaccgtt gctaggcacc agcatctacc acgtcagccc
     4021 cacggcgctg tggtggggct gcggcctcgt cggcctcatg tcggccgggc tggcgcttcg
     4081 agccggacat ttccc
//
LOCUS       DATDBZ010000313         2604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2332680, whole genome shotgun sequence.
ACCESSION   DATDBZ010000313 DATDBZ010000000
VERSION     DATDBZ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332680"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VIX62_07575"
     CDS             complement(<1..1347)
                     /locus_tag="VIX62_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010062357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEY5860127.1"
                     /translation="MSIQTATSNGDAELPAPADGDFAGRAEYGREARRRTPRSAHEGW
                     APPPDRADPVTLIEEQATTRVPELVPIRHARMMVSPFTFYRGAALLMAADLAGTQVSG
                     ITTQICGDAHLSNFGVFGTPERTLVFDMNDFDETLPGPWEWDVKRLAASFEVAGRSRG
                     FTDEERREINLTVVRGYRERMLRAAESRVLDAWYDRLDADQLMEYATAGQESKRSADK
                     LTKRADAALAKARTKDSTRAVAKLTEVVDGQLRIAADPPLIVPIEQLDPIGRTREQEE
                     DWMREVLASYQSTLQQQNHPMREFTYVHLARKVVGVGSVGTRAWIVLVRGRDDADPLL
                     LQAKQAEASVLERFLPASEYDNHGERVVRGQRLMQAASDIFLGWHRQAGFDGVDRDFY
                     IRQLHDWKGSADVDTMNTKVATLYARVCGETLARAHARAGDRVAIAAYLGRSDRFDR"
     gene            complement(1408..>2604)
                     /locus_tag="VIX62_07580"
     CDS             complement(1408..>2604)
                     /locus_tag="VIX62_07580"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEY5860128.1"
                     /translation="SIPAPSGFLGTVDDMWFRWITDMGLPGPDRGTGGRYLLVGPGYD
                     GPLSEGGFYVSHSRTTRVILLLRAFMIDNDPRVALDVIRNGLRISPYTPGGPGTSVGS
                     FLAGDSPLAPPTAPSETTFVEGSHVSFNTVPPSDSTYWELLDELVQDEPVGSGDPDLL
                     GLLAAVGISKGRKFEPDERMRRILDEALTVGNATARTLAFAPRAAEGWAYYPGSQWMN
                     MLFAGGYEFLAPPPEITPQGVVIDPGNGARKLNARIAFFYPYTGITPAMCMRLTGIGS
                     QYLIAMRDADGAFLDGGRDYRITLPPGIPQSRFWSVILYDRQTRSMLQTSQPKPSFGS
                     QSGTVQTNADGTTTIHIGPNAPAGAESNWMQSIPGKGHFVVLRFYNPLQSFFDKTWRP
                     SEIEPV"
BASE COUNT          392 a          894 c          887 g          431 t
ORIGIN      
        1 tcggtcgaat cgatcactcc ggcccaggta cgcggcgatg gcgacccgat cgcccgcacg
       61 tgcatgcgcc cgtgcgagcg tctcgccgca cacgcgtgcg tagagcgttg ccaccttggt
      121 gttcatcgtg tcgacgtcgg cggagccctt ccagtcgtgc agctgccgga tgtagaagtc
      181 gcggtcgacg ccgtcgaacc cggcctggcg atgccagccg aggaagatgt cgctcgccgc
      241 ctgcatcagc cgttgcccgc gcacgacccg ctcgccgtga ttgtcgtact cgctcgccgg
      301 caggaaccgc tccagcaccg acgcctcggc ctgcttggcc tgcagcagca gcgggtcggc
      361 gtcgtcgcgg ccgcgcacca gcacgatcca cgcccgcgtg ccgacacttc cgacgcccac
      421 caccttgcgc gccaggtgca cgtaggtgaa ctcccgcatc ggatgattct gctgctggag
      481 cgtggactgg tacgacgcca gcacctcccg catccagtcc tcctcctgtt ctcgggtgcg
      541 cccgatcggg tccagctgct cgatcggcac gatcaggggc gggtcggcgg cgatccgcag
      601 ctggccgtcg accacctcgg tgagcttggc cacggcacgg gtgctgtcct tcgtccgcgc
      661 cttcgcgaga gcggcgtcgg cgcgcttcgt gagcttgtcg gcgcttcgct tggactcctg
      721 tcccgcagtg gcgtactcca tcaactggtc ggcgtcgagc cggtcgtacc aggcgtcgag
      781 cactcgcgac tcggcggcgc gcagcatccg ctcgcgatag ccgcgcacga ccgtgaggtt
      841 gatctcccga cgctcctcgt cggtgaagcc acgactgcgc ccggcgacct cgaagctcgc
      901 cgcgagccgc ttcacgtccc actcccatgg gcccggcagc gtctcgtcga agtcgttcat
      961 gtcgaacacc agcgtgcgtt cgggggtgcc gaacaccccg aagttcgaca ggtgcgcgtc
     1021 accgcagatc tgggtcgtga tccccgacac ctgcgtgccg gcgaggtcgg ccgccatcag
     1081 cagcgcggca ccgcgataga acgtgaacgg cgacaccatc atccgcgcat gccggatggg
     1141 cacgagttcg gggacccgag tggtcgcctg ctcctcgatc aacgtcacgg gatccgcacg
     1201 atcgggcggc ggtgcccagc cctcgtgcgc agaacggggc gtacgccgcc tggcctcccg
     1261 gccgtactcg gcgcggccgg cgaagtcgcc gtcggccggc gcgggcagtt cagcgtcgcc
     1321 gttgctcgtt gcggtctgaa tggacattcc aggacctccg atccctgtag tgctcgcggt
     1381 tcggcgcgac gtgcagactg cctcgcccta gaccggttcg atctcgctcg gtcgccaggt
     1441 cttgtcgaag aacgactgca gcgggttgta gaaccgcagc accacgaagt gacccttccc
     1501 tgggatcgac tgcatccagt tggactcggc acccgcgggt gcgttcggtc cgatatggat
     1561 ggtggtcgta ccgtcggcgt tcgtctgcac ggtgcccgac tggctgccga atgacggctt
     1621 cggctggctg gtctgcagca tcgatcgtgt ctggcggtcg tagaggatca ccgaccagaa
     1681 ccggctctgg gggatgccgg gcggcagtgt gatccggtag tcgcgccccc cgtcgaggaa
     1741 ggcgccgtcg gcgtcgcgca tcgcgatgag gtactgggag ccgatgccgg tcaggcgcat
     1801 gcacatcgcg ggcgtgatgc ccgtgtacgg atagaagaac gcgatgcgcg cgttgagctt
     1861 ccgtgctccg ttgccggggt cgatcacgac gccctggggt gtgatctcgg gcggcggggc
     1921 gaggaattcg tacccgcccg cgaagagcat gttcatccac tgggatccgg gatagtacgc
     1981 ccagccttcc gcagcgcgtg gcgcgaacgc gagcgtgcgt gcggtcgcgt tgcccaccgt
     2041 caacgcctca tcgaggattc gacgcatccg ctcatcgggc tcgaacttcc gccccttcga
     2101 gatccccacg gcggcgagga ggccgagcag gtcggggtcg cccgaaccga cgggctcatc
     2161 ctggacgagc tcgtcgagca actcccagta ggtcgaatcg ctcggcggca cggtgttgaa
     2221 ggacacgtgg gagccctcca cgaacgtggt ctccgaggga gcggtgggcg gcgcaagcgg
     2281 ggaatcacca gcgaggaacg agcccaccga ggtcccgggc ccgcccggcg tgtacggaga
     2341 gatgcggaga ccgttgcgga tgacgtcgag tgccacccgg gggtcgttgt cgatcatgaa
     2401 tgctcgcagc agcaggatca cccgagtcgt cctggagtgc gacacgtaga acccgccctc
     2461 ggacagcggc ccgtcgtagc ccggcccgac gagcaggtac cgtccgccgg ttccgcgatc
     2521 cggcccgggc agccccatgt cggtgatcca ccggaaccac atgtcgtcga cggtgccgag
     2581 gaatcccgac ggtgccggga tcga
//
LOCUS       DATDBZ010000314         5928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1544319, whole genome shotgun sequence.
ACCESSION   DATDBZ010000314 DATDBZ010000000
VERSION     DATDBZ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544319"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..876)
                     /locus_tag="VIX62_07585"
     CDS             complement(37..876)
                     /locus_tag="VIX62_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5860129.1"
                     /translation="MLGIALALASAASWGTADFLGGLTTKQLSILVVSAVSQFAGLVF
                     MVLILVVLARGPADSHVIVLGLAAGVCGVVGLAALYSGLAIGPMGVVAPITALSGVVS
                     VCIGLARGDRPSGLQLAGIVVAIVGVVLASRTPEGHGEPVTARAIVLAVIAAVSLGVL
                     VVLLDEGGRIDPAWTSTMVRVSALSLLAVAVLVRRPSFAMARIQLGTLVVVGLLDNGA
                     NLLFAIASSTGQLLSVIAVLAALYPVSTVLLARIVLHERLARVQVAGVVLAFVGIALL
                     ALG"
     gene            complement(911..2125)
                     /locus_tag="VIX62_07590"
     CDS             complement(911..2125)
                     /locus_tag="VIX62_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5860130.1"
                     /translation="MTRTLFRGGRVFDGSGAAPAEADVVIEDGRIIEVGSGLDGDDAV
                     GCAGKALLPGLFDCHVHLAFRYEDFDEYARMQEPFSLAFFRMAHNLRITLALGITTVR
                     DASGADAGLRAAVEEGTLVGPRMQISVNMLSMTGGHNDPWLPSGVEGVFGTPYPGMPS
                     GVCDGVDEVSKTVREMIRAGADVIKIASSGGFLSPADDPMEPNYSQDEVEAIVRTAAD
                     LGTWVMSHAHGPEGIKRAVRAGVRSIDHGTYLDAEGAAMMVERGTWLVPTLTAGDATE
                     ALAADEKVQPAVRDKLRGLGRPEFDAMRLAADAGVKVAMGTDCPVAPHGTNLNELRHM
                     AANGFTPEQALHAATKSAAELMGLQDELGTIEPGKRADLVVIDGDPLEFAGYEDRIEQ
                     VWKDGLRAVPFA"
     gene            2217..3401
                     /locus_tag="VIX62_07595"
     CDS             2217..3401
                     /locus_tag="VIX62_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5860131.1"
                     /translation="MDIRLNADDAALQRTARGFTRDVLMPLEDECEEHDGLTPESHAA
                     AKRAVLDAGFNAINHPVADGGQGYDTFQQMLIEEQWGHATGALWDIPWRPSIPLQRCT
                     DEQRARYLHPAIRGERRDAYAITEEGAGSDVSMVATTARRDGDGWVLDGEKWHVTSGD
                     VADFFLVHAHVDADPAKATVFLVDKDTPGVRLVRTPKYTHTFVFEHPIFAFDGVRLGD
                     DAILGEVGEGFELTKDWFVEERIMIGARTMGASARALQLALDFAQDRRQFGRAIVDFQ
                     AIEFMLADMAAEIMAAKSLLYRVCWEAARGDADRKQTHAMASAVKLVCSETAGRVIDK
                     AVQIFGGRGYMRDEPVERLYRELRVDRIWEGTSEIQRVVIGNELRKRGAELYTGWPVE
                     GA"
     gene            complement(3398..3721)
                     /locus_tag="VIX62_07600"
     CDS             complement(3398..3721)
                     /locus_tag="VIX62_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860132.1"
                     /translation="MIAVVSTVAGAVLGLFLWLFHLRPTSALLGDYAYELAHDGGFRE
                     QVLWLTATLGAVGIVAALFTVVGGRPKPSTVVCLLLGAIALSYPVLALTGALGAPVAP
                     PLFPG"
     gene            complement(3838..4542)
                     /locus_tag="VIX62_07605"
     CDS             complement(3838..4542)
                     /locus_tag="VIX62_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860133.1"
                     /translation="MRRSLPVIAALVAVSFLAACTDDAPQAEPSDAFPNSPTTATPTF
                     SGGGTPSPAAVSPTPPAIPDDPPKTKGPANATCVQGWVTPPAGTPMFTDPLGLIRRTA
                     PVAGDFEVVDMRMFVGPESPPSVGDDAKGYLQDIRRWYIKLFVPSDLRYQGRFIVEQR
                     VFGRGVAAVAPYDTEGFSSPDWSGFQYDSAQRLRKPYPGLPGRWSGIRYDFVRGGAGL
                     SIPGLPDEVTGCLDGT"
     gene            4631..>5928
                     /locus_tag="VIX62_07610"
     CDS             4631..>5928
                     /locus_tag="VIX62_07610"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5860134.1"
                     /translation="MSDHVHPHDEERGDAHPLPVARRRFLQTTGMALAGGVLFSCTGG
                     RTIPKVSDDPVTPAIDTATPIKRVVYIMLENRSFNNVFGRFPGVKGGTETGVEFGAEK
                     PLIDCPEWLPGDLPHDRAAYLTCVNEGAMDGFGIGAYGSTYAYTVFNEHQIPNYWKWA
                     KEYALSDNFYASAAGPSYANHYYFIAGQSGGVIDNPENIETRFRDGKPYKSWGCDAYG
                     EDVFVFAKDAKGNLSKHDTCFSFKTVGQQLSEQDVDWAYYSAVPGQPGYMWNAYNGIS
                     DVFHTDMWHQHMRPVDNIVKDIKANKLPAVTWVTPRFELSDHPPQSTGFSHNWVTDIV
                     NAVMTSDAWEHTAIFLTWDEWGGFYDPIMPPEVDPIGLGIRVPLLTISPYTQRGVIDT
                     ELGEFSTPLRFISDNWGLEPLTPRIANTHNFDHVFDFNQKP"
BASE COUNT         1048 a         2058 c         2008 g          814 t
ORIGIN      
        1 ggacaacgcc caaggccccc cccagctcag gtggcgctag ccgagcgcca ggagcgcgat
       61 cccgacgaag gccaggacca ctccggccac ctggacgcgc gcgagccgtt cgtgcagcac
      121 gatccgcgcc agcaggaccg tgctgacggg atagagcgcc gcgagcacgg cgatcacgct
      181 gagcaactgt cccgtcgagg acgcgatggc gaacagcagg ttggcgccgt tgtccaacaa
      241 ccccaccacc acgagcgtcc ccagctggat gcgcgccatc gcgaacgacg gacggcggac
      301 gagcaccgcg acggccagga gcgagagcgc gctgacgcgc accatggttg aggtccacgc
      361 ggggtcgatc cggccacctt cgtccagcag caccaccagg acgccgaggc tcaccgcggc
      421 gatcaccgcc agcacgatcg cgcgggcggt gacgggttcc ccgtgcccct cgggcgtgcg
      481 ggaggcgagg acgacaccga cgatcgccac gacgatgccc gcgagctgga gcccgctcgg
      541 acggtcgccc cgcgcgagac cgatgcacac cgacaccacg cccgagagcg ccgtgatcgg
      601 ggcgaccacc cccatcggtc cgatcgccag gcctgagtag agcgccgcca gcccgacgac
      661 gccgcacacc cccgcggcca ggcccaggac gatgacgtgg ctgtcggcgg gtccccgcgc
      721 cagcaccacg aggatcagca ccatgaacac gaggcccgcg aactgggaca ccgccgagac
      781 caccaggatc gacagctgct tggtggtgag gccaccgagg aagtcggcgg tcccccagga
      841 ggctgcggag gccagggcga gcgcgatgcc gagcatcggg cgagcctacc ggcgcgaggt
      901 caagggagcc tcacgcgaac ggcacggccc ggaggccgtc cttccacacc tgctcgatcc
      961 ggtcttcgta cccggcgaac tcgagcgggt cgccatcgat caccacgagg tccgcgcgct
     1021 tgccgggctc gatcgtgccc aactcgtcct gcagccccat cagctcggcg gcgctcttcg
     1081 tggcggcgtg cagcgcctgc tcgggcgtga agccgttggc cgccatgtgc cggagctcgt
     1141 tgaggttcgt accgtgcggg gccaccgggc aatcggtccc catggccacc ttcacccccg
     1201 catcggcggc caggcgcatc gcgtcgaatt ccggacgccc caacccacga agcttgtcgc
     1261 gcacggcggg ctgcaccttc tcgtcggcgg cgagcgcctc ggtggcgtca ccggcggtga
     1321 gggtgggcac gagccatgtg ccccgctcca ccatcatcgc ggcgccttcg gcgtcgaggt
     1381 aggtcccgtg gtcgatcgaa cggacgccgg cgcgcaccgc gcgcttgatc ccctcggggc
     1441 cgtgcgcgtg cgacatcacc cacgtcccca ggtcggcggc ggtgcggacg atcgcctcga
     1501 cctcgtcctg ggagtagttc ggttccatgg ggtcgtcggc cggcgagagg aacccgccgg
     1561 aggacgcgat cttgatgacg tcggcccccg cgcggatcat ctcgcgcacg gtcttgctga
     1621 cctcgtcgac cccgtcgcac accccggagg gcatccccgg gtacggcgtg ccgaagaccc
     1681 cttcgacgcc gctcgggagc caggggtcgt tgtggccccc ggtcatcgac agcatgttga
     1741 ccgagatctg catccgcggg ccgacgagcg tgccctcctc gacggccgcc cgcaggcccg
     1801 cgtcggcccc ggaggcatcg cgcaccgtgg tgatgcccag cgccaaggtg atgcggaggt
     1861 tgtgggccat gcggaagaac gccagcgaga acggctcctg catacgcgcg tattcgtcga
     1921 agtcctcgta gcggaacgcg agatgcacgt ggcagtcgaa caatcctggg agcaacgcct
     1981 tgccggcgca tccgacggca tcgtcgccgt cgaggccgga tccgacttcg atgatccggc
     2041 cgtcctcgat cacgacatcg gcctcagccg gcgcggcgcc cgagccgtcg aacacccgtc
     2101 ctcctcggaa cagtgtcctc gtcacgttca ccctcctcgc gatcggcgac ctagactgcg
     2161 cgggcaaccg cccggtggcg gaccctaccg tgcccagcat gaacgaggag tgacccgtgg
     2221 acatccgcct caatgccgac gacgcggcac tgcaacgcac cgctcgtgga ttcacccgcg
     2281 acgtgttgat gccactcgag gacgagtgcg aggagcatga cggcctcacg cccgagagcc
     2341 acgcggcggc gaagcgagcc gtactcgacg ccgggttcaa cgcgatcaac catccggtcg
     2401 ccgacggcgg ccagggctac gacacgttcc agcagatgct gatcgaggaa cagtggggcc
     2461 acgccacggg cgccctgtgg gacatcccat ggcgtccgtc gatccccttg caacggtgca
     2521 ccgacgaaca gcgggcgcgc tacctgcacc cggcgatccg cggtgagcgt cgtgacgcgt
     2581 acgcgatcac cgaggagggc gcaggctccg acgtctcgat ggtggccacc accgctcgcc
     2641 gggacggcga cggttgggtg ctcgacggtg agaagtggca cgtcacgagc ggcgacgtcg
     2701 ccgacttctt cctggtgcac gcccacgtcg acgccgatcc ggcgaaggcg accgtgttcc
     2761 tggtggacaa ggacaccccg ggcgtgcggc tggtccgcac cccgaagtac acgcacacgt
     2821 tcgtgttcga gcatccgatc ttcgccttcg acggggtgcg cctgggcgac gatgcgatcc
     2881 tgggagaggt gggcgagggg ttcgagctca cgaaggactg gttcgtcgaa gagcggatca
     2941 tgatcggcgc ccgcacgatg ggcgccagcg cacgcgccct gcagctcgcc ttggacttcg
     3001 cgcaggaccg gcggcagttc ggacgggcga tcgtggactt ccaggcgatc gagttcatgc
     3061 tcgccgacat ggcggccgag atcatggcgg cgaagtcgct cctgtaccgc gtgtgttggg
     3121 aggccgcgag aggcgacgcc gatcggaagc agacccacgc gatggcgagc gccgtgaagc
     3181 tggtatgcag cgagacggcc ggccgggtga tcgacaaggc ggtgcagatc ttcggcggcc
     3241 gcggctacat gcgcgacgaa cccgtcgagc gcctgtaccg ggagttgcgc gtggatcgca
     3301 tctgggaagg cacgagcgag atccagcgtg tggtgatcgg caacgagctg cggaagcgcg
     3361 gcgccgagct gtacaccggc tggccggtcg agggcgccta acccgggaac agcggcggag
     3421 cgaccggtgc gccgagtgcg ccggtgagcg ccagcaccgg gtagctcaac gcgatcgcac
     3481 ccagcagcag gcacacgacc gtcgagggct tcggtcgccc acccaccacg gtgaagaggg
     3541 cggcgacgat ccctacggcg cccagcgtgg cggtgagcca caggacctgt tcgcggaacc
     3601 caccgtcgtg cgcgagttcg taggcgtagt cacccaacag cgccgaggtg ggccgaaggt
     3661 ggaagagcca gaggaacagc cccaggaccg caccggcgac ggtactgacg acggcgatca
     3721 tcccgagtgg attcgaagcg acgggtcgat ggcgggctac gacgtccata cgggtatgca
     3781 atcacccggt cccgaacggc gcatcggccg gaagacctga gccgaaggtc gcgggtgcta
     3841 cgtgccgtcg aggcagccgg tgacctcatc cggcagcccg gggatcgaga ggccggcgcc
     3901 gccgcggacg aagtcgtatc ggatgccgga ccacctgccg ggcagccccg gatagggctt
     3961 gcgcaaccgc tgcgccgagt cgtactggaa cccgctccag tccggcgagg agaacccttc
     4021 ggtgtcgtac ggagcgacgg cggcgacccc gcggccgaac acccgctgct cgacgatgaa
     4081 gcgaccctga tagcgcagat cgctgggcac gaagagcttg atgtaccaac gccggatgtc
     4141 ctgcaggtag cccttggcat catcaccgac cgaaggcggt gactccggcc ccacgaacat
     4201 gcgcatgtcc accacctcga agtccccggc caccggcgcg gttcgccgga tcagcccgag
     4261 cggatcggtg aacatcgggg tgccggccgg gggcgtgacc cagccctgga cgcacgtggc
     4321 gttcgccggc cccttcgtct tcggcggatc gtcggggatc gcgggcggcg tcggtgacac
     4381 cgcggcggga ctcggtgttc cgccgccgga gaaggtgggt gtcgccgtcg tggggctgtt
     4441 ggggaacgca tcggacggtt cggcctgcgg ggcgtcatcg gtgcacgccg caaggaacga
     4501 gaccgcgacc agggccgcga tgaccgggag ggatcgtcgc acccgtgaat cctacgcatc
     4561 cgatgtggcg aacccgttgc gagcccgcaa cttcccccgt tctcgcctgg atgtttggta
     4621 ggaatggtgg gtgagcgacc acgtccaccc ccacgacgag gagcggggcg acgcccatcc
     4681 cctcccggtc gccaggagga gattcctgca gaccaccggc atggcgttgg ccggcggcgt
     4741 cctgttctcg tgcaccggcg gccgaaccat cccgaaggtc agcgacgatc cggtcacgcc
     4801 cgcgatcgac accgccaccc cgatcaagcg cgtggtctac atcatgctcg agaaccgcag
     4861 cttcaataac gtgttcggac ggttccccgg cgtcaagggc ggcacggaga caggcgtcga
     4921 gttcggggcg gagaaaccgc tgatcgattg ccccgagtgg ctccccggag acctgccgca
     4981 cgaccgcgcc gcctacctca cctgcgtgaa cgagggagcc atggatggct tcggcatcgg
     5041 ggcctacggc tccacatacg cgtacacggt cttcaacgaa caccagatcc cgaactactg
     5101 gaagtgggcc aaggaatacg ccctgtccga caacttctac gcgtcggcgg ccgggccgag
     5161 ctacgccaac cactactact tcatcgccgg ccagtccggt ggcgtgatcg acaaccccga
     5221 gaacatcgag acgcggttcc gcgacggtaa gccctacaag agctggggct gcgacgccta
     5281 cggcgaggac gtcttcgtgt tcgccaagga cgccaagggc aacctgagca agcacgacac
     5341 ctgcttcagc ttcaagaccg tgggtcaaca gctgtcggaa caggacgtcg actgggccta
     5401 ctactcagcg gtcccggggc agcccgggta catgtggaac gcctacaacg ggatctccga
     5461 cgtgttccac accgacatgt ggcaccagca catgcggccg gtggacaaca tcgtgaaaga
     5521 catcaaggcc aacaagctcc cggccgtcac atgggtcaca cccaggttcg agctctccga
     5581 ccacccgccg cagtcgacgg gcttctcgca caactgggtc accgacatcg tgaacgccgt
     5641 gatgaccagc gatgcgtggg aacacaccgc gatcttcctc acctgggacg agtggggagg
     5701 cttctacgac ccgatcatgc cgcccgaggt ggacccgatc gggctcggga tccgcgtgcc
     5761 cctgctgacg atcagcccgt acacgcaacg cggcgtgatc gacaccgaac tcggtgagtt
     5821 ctcgacgccg cttcggttca tctccgacaa ctgggggctc gagccgttga cgccacgcat
     5881 cgccaacacg cacaacttcg atcacgtgtt cgacttcaac cagaagcc
//
LOCUS       DATDBZ010000315         2867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1949950, whole genome shotgun sequence.
ACCESSION   DATDBZ010000315 DATDBZ010000000
VERSION     DATDBZ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949950"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VIX62_07615"
     CDS             complement(<1..471)
                     /locus_tag="VIX62_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY5860135.1"
                     /translation="MSENQRRSEPQLAVIILAAGQGTRMKSTLPKVLHRIGGRPLLGH
                     VLDTARTLGPQSILVVVRHERDLVADVVVAIAPEIVVVDQDEIPGTGRAVEMALAHVP
                     DFAGDVLVLSGDVPLLDDETLARLIRTHRESGAAATVRSAVVGDATGYGRVIRDS"
     gene            complement(473..544)
                     /locus_tag="VIX62_07620"
     tRNA            complement(473..544)
                     /locus_tag="VIX62_07620"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(510..512),aa:Gln,seq:ttg)
     gene            627..1124
                     /locus_tag="VIX62_07625"
     CDS             627..1124
                     /locus_tag="VIX62_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5860136.1"
                     /translation="MAHETDEVDRIVEAWVAQRPDLDFSPLEVLSRVDRLSRHLDRAR
                     RDAFRRSDLEPWEWDVLSALRRAGEPFQLSPKALLQQTLVSSGTMTNRIDRLVGRRLV
                     HRESDPDDGRSILVTLTGDGRTRVDAAITRLVDAEALLLDTLSRSDRERLAGLLRKLS
                     LGFDA"
     gene            1289..2548
                     /locus_tag="VIX62_07630"
     CDS             1289..2548
                     /locus_tag="VIX62_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEY5860137.1"
                     /translation="MDEARGRVAFDAPDTLDHLIEIADMATMEAAQKLVGVNVLRREA
                     LADAARHGRVLTDVVERSVRLEIAAALRVTEHEAGAMMALGEALVERFPTVLDSFASA
                     RMTEPHARYLVESVAVVEPQFHPRILPAAIELAEAEPVGTFRRRLRALIETVRSTTLS
                     ERYEDAARGRRVVLQPDHDAMTWVMTLMPAVEAQAIWGRATRIAKVILAEEGETRTLD
                     QIRADVVADLLIEGDTDLHPADARGIRATVTVTVPVLALLEGAPEGTEPAVVEGVGPI
                     PIERARELAGGSEGWMRVLTHPETGMVRSVGRDRYSPPLSLRRLVKWRADRCMEPGCA
                     IPASRCEVDHRLAWEHGGQTRLDNLNPFCKGHHILKHHGGWTVRDVTGSFGAVEWTSP
                     TGRRYIVQPERRVPVFRTQPDEDPPPF"
     gene            2557..>2867
                     /locus_tag="VIX62_07635"
     CDS             2557..>2867
                     /locus_tag="VIX62_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEY5860138.1"
                     /translation="MTGQVEFDVPSADSLPDPTPSGIDPMELEITLRVLAQLHEVDPE
                     HPDYSTVRRATANMFKSAKKERRLQKRALIADADRSVIAATATGAPDRIDDETRGIPL
                     ET"
BASE COUNT          458 a          990 c          993 g          426 t
ORIGIN      
        1 cgagtcgcgg atgacgcggc cgtagccggt cgcgtcgccg accactgcgc tccgcacggt
       61 cgccgccgcg ccgctctcac ggtgggtgcg gatcaggcga gcaagcgtct cgtcgtcgag
      121 cagcggcacg tcgccgctga gcaccagcac gtctccggcg aagtcgggca cgtgcgcgag
      181 ggccatctcg accgcgcgac cggtgcccgg gatctcgtcc tgatcgacca ccacgatctc
      241 gggggcgatg gcgacgacga cgtccgcgac cagatcgcgc tcgtggcgca ccacgacgag
      301 gatgctctgc gggccgagcg tgcgggccgt gtcgagcacg tgccccagca gcgggcggcc
      361 accgatccgg tgcaggacct tggggagcgt ggatttcatc cgcgttccct ggccggcggc
      421 gaggatgatc acggcgagct gcggttctga cctgcgctgg ttctcagaca tgctccgccg
      481 ccaggattcg aacctagacc taacagctcc aaaggctgtc gtgctgccat tacaccacgg
      541 cggaccgcgg cccccgcctg gtgagggagc ggccgcccgt caagtctgcc agacggtcgc
      601 gcggcccctg ccgcgataat gggcacatgg cgcacgagac ggacgaagtc gatcggatcg
      661 tcgaagcatg ggtcgcgcag cggcccgatc tcgacttctc cccgctcgag gtgctctcgc
      721 gcgtcgaccg cctctcgcgc cacctcgacc gcgcgcgccg cgacgcgttc cggcgcagcg
      781 acctcgagcc ctgggagtgg gatgtcctgt cggccctccg ccgcgcgggc gagccgttcc
      841 agctgagccc gaaggccctg ctgcagcaga cgctcgtctc gagcggcacg atgaccaacc
      901 gcatcgaccg gctcgtcggg cggcggctcg tgcatcgcga atccgacccc gacgatggcc
      961 gcagcatcct ggtcaccctc accggagacg gacgcacacg ggtggatgcg gcgatcacac
     1021 gactcgtgga tgccgaagcc ctcctgctgg acaccctctc gcgctccgat cgggagcgac
     1081 tggccggcct gctgcgcaag ctcagcctcg gcttcgacgc ctgactgttg cacgatcggg
     1141 tgatgcgcgg gccccttccg atgtcgtagg tacgttctaa tctggagtca tgttcttcga
     1201 tccgtcgctt cccccggacc cgcagggtct agaggaggcg tgggatgctc tcgacctccg
     1261 tggggccgtc tgcgaggagc cgcctgaggt ggatgaggca cgtgggcgag tcgcgttcga
     1321 tgcgcctgac acgctcgatc atctgatcga gatcgcggac atggccacga tggaggcggc
     1381 gcaaaagctg gtcggggtta acgtgctccg tcgggaggcg ctggcggatg ccgcccgcca
     1441 cggtcgcgtg ctgaccgatg tggtcgagcg gtcggtgcgg ctggagatcg ccgccgctct
     1501 gcgggtcacc gagcacgaag ccggggcgat gatggcgctg ggcgaggcac tcgtggaaag
     1561 gtttccgacc gttctggact cgttcgcgag tgcgcgcatg acggagccgc acgcccggta
     1621 cctggtggag tcggtggccg tggtcgaacc ccagttccat ccgcggatcc tgccggcggc
     1681 gatcgagttg gcggaggcgg agccggtcgg caccttccgc cgtcggctgc gcgcgctgat
     1741 cgagacggtt cgttcgacga cgctgtccga gcggtacgaa gacgcagccc gggggcgccg
     1801 ggtggtgctg caacccgatc acgacgcgat gacctgggtg atgacactga tgcccgcggt
     1861 agaggcgcag gcgatctggg gcagggcgac gaggatcgcg aaggtgatcc tcgccgagga
     1921 gggtgagacc cggacactcg accagatccg tgccgacgtg gtcgcggatc tgctcatcga
     1981 gggcgacacc gacctgcacc cggccgacgc gcgcggcatc cgcgcgaccg tcaccgtcac
     2041 cgttcccgtg ctcgccctcc tcgagggggc accggagggc accgaaccgg ccgtggtgga
     2101 gggtgtcggc ccgatcccga tcgaacgggc acgtgaactc gccggcggat cggaggggtg
     2161 gatgcgggtc ctcacccacc ccgagacggg gatggtccgc tcggtcggtc gcgaccgata
     2221 ctccccgccg ctcagcctgc ggcgcctcgt gaaatggcgt gccgaccggt gcatggaacc
     2281 gggatgcgca ataccggcat cccgttgcga agtcgaccat cgcctcgcgt gggaacacgg
     2341 cggacaaacc aggctcgaca acctgaatcc gttctgcaag ggccaccaca tccttaagca
     2401 ccacggcggg tggaccgtgc gagacgtcac gggaagcttc ggcgcggtcg aatggacctc
     2461 acccaccggc agacgctaca tcgtccaacc cgaacgacgc gtacccgtgt tccgcacgca
     2521 gcccgacgaa gacccgccac ccttctgatc ccggcgatga cgggccaggt agaattcgat
     2581 gtgccctccg ccgattccct ccccgacccg actccgagcg gcatcgaccc gatggagctc
     2641 gagatcaccc ttcgagtgct cgctcagcta cacgaggtcg acccggagca tcccgactac
     2701 agcaccgtcc ggcgcgcgac cgcgaacatg ttcaagtcgg ccaagaagga gcggcgcctg
     2761 cagaagcggg cgctgatcgc ggatgccgac cgttcggtga tcgcggccac cgcgaccgga
     2821 gcgcccgacc ggatcgatga cgagacgcgc ggcatcccgc tcgagac
//
LOCUS       DATDBZ010000316         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2962221, whole genome shotgun sequence.
ACCESSION   DATDBZ010000316 DATDBZ010000000
VERSION     DATDBZ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2962221"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VIX62_07640"
     CDS             complement(<1..330)
                     /locus_tag="VIX62_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY5860139.1"
                     /translation="MSETEPESSTAPVGVLVMAYGTATGPDDIERYYTDIRGGRTPSP
                     EHLQELKDRYAAIGNVFPLLDTTAAQAQGLVERLNEASEVDGDDRTYRAYLGMKHSPP
                     FIPDGVEA"
     gene            complement(327..1382)
                     /gene="hemE"
                     /locus_tag="VIX62_07645"
     CDS             complement(327..1382)
                     /gene="hemE"
                     /locus_tag="VIX62_07645"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY5860140.1"
                     /translation="MSSDLFNDRFLRACRRLPVDRTPVWFMRQAGRYLPEYRELRGDR
                     DILDTCKIPEQVVEISLQPLRRMPLDAAIVFSDIMVPLAGVGVDVRIEPGRGPVVDTP
                     VRSASDVARIRPLEPEQDVPEALEAIRLLRKELSVPLVGFAGAPFTLASYLVEGGPSR
                     SHERTKALMLGEPQTWDALMRALVAVVEPHLRAQVAAGAQALQVFDSWVGALEPEDYR
                     ASVLPHMRGLFDRLIDLDVPVIHFGVGTGELLPLLREAGGDVIGVDWRTPLDEAWHRV
                     GGSAHVAVQGNLDPAVLLASWDAVEAKALRVLERAAGRDGHVFNLGHGVLPTTAPETL
                     ERLVDLVHTTTQRGTTT"
     gene            complement(1425..>2679)
                     /locus_tag="VIX62_07650"
     CDS             complement(1425..>2679)
                     /locus_tag="VIX62_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860141.1"
                     /translation="VDAVDEGRKGQLQPNRTYEPHSRPVRDVPTRKLRHYFFKDHPSK
                     RRELDMLIRRLQRMDVEVYRLNAPLEVSDLRPFMEEPHRETLPAGTYWVPMAQRQKRW
                     IQAALNEDPFNPTNEAYRVSSWSLPLAYNLDGAMSGLTLDPDADLVDPVAEPAPWTLA
                     SPLPSVAILHLSRDFTAYENIGHTRWLFKTQWGLPYSQINPEDVAGGLDGIDVLVVPA
                     GGINEGLRKLGEAGQRELVRWVNRGGRLVGWRYGAARLAYALGISRARYEGLPGGMDG
                     PMVKALIDADSPLGEGVGRSAWPLMDTRGMFAPNGVSPVHFPTRESGKFRVSGVRRGT
                     HWLMGTTAVADEPVGRGRSIVFSFDPAYGGGSEGTQKILFNAILGPDPAGRRPGEPVR
                     FDPEEVARRLAETTDWVDEPTPDVH"
BASE COUNT          445 a          917 c          895 g          422 t
ORIGIN      
        1 ggcttcgact ccgtcaggga tgaacggcgg cgagtgcttc atgcccagat acgcccggta
       61 ggtgcgatcg tcaccatcga cttcgctggc ctcgttcagt cgctcgacca acccctgtgc
      121 ctgcgccgcc gtcgtgtcca atagtgggaa cacgttgccg atcgcggcat agcggtcctt
      181 cagttcctgc agatgctcgg ggctcggtgt gcggccgccg cggatgtcgg tgtagtagcg
      241 ctcgatgtcg tcggggccgg tcgccgtgcc gtaggccatg acgaggacac ccacgggggc
      301 cgtcgacgac tcgggttcgg tctcgctcac gtggtggttc ctctctgtgt ggtcgtgtgg
      361 accaggtcca cgagtcgttc gagggtttcg ggagccgtcg tcggcaacac gccgtggccc
      421 aaattgaaca cgtgcccgtc tcggccggcc gcgcgttcca gcacgcggag tgccttcgcc
      481 tcgacggcgt cccaggaagc gagcaacacg gcggggtcca ggttgccctg caccgccacg
      541 tgggccgaac ccccgacgcg atgccacgct tcatccaacg gggtgcgcca gtcgacgccg
      601 atgacgtcgc caccggcttc gcggagcaag ggaagcaact caccggtgcc cacaccgaag
      661 tggatcaccg gcacgtcgag atcgatgagc cgatcgaaca acccgcgcat gtgtggcagc
      721 acgctcgcgc ggtagtcctc gggctcgagc gcaccgaccc acgagtcgaa cacctgcagc
      781 gcctgcgcac cggctgcgac ctgggcacga aggtgcggtt cgacgacggc gaccagcgct
      841 cgcatcaggg cgtcccacgt ctgcggttcg cccagcatca gggccttggt ccgctcgtgg
      901 ctgcgcgaag ggcccccttc caccaggtag gaggccaggg tgaacggcgc cccggcgaaa
      961 ccgaccaacg gcaccgacag ttccttgcgg agcagccgga tcgcctcgag tgcttcgggc
     1021 acgtcctgct cgggttccag ggggcggatg cgtgcgacgt cagacgccga tcgcaccggc
     1081 gtatcgacga ccggcccgcg acccggttcg atccgcacgt cgacgcccac cccggcgagc
     1141 ggcaccatga tgtcgctgaa gacgatcgcc gcgtccagcg gcatccggcg caatggctgc
     1201 aacgagatct cgacgacctg ttcggggatc ttgcaggtgt ccaggatgtc gcgatcgccg
     1261 cgcaactcgc ggtattcggg caggtaacgg cccgcctggc gcatgaacca gacgggcgtg
     1321 cgatccaccg gcagacgccg acaggcgcgc aggaaccgat cgttgaacag gtccgaactc
     1381 acggacccag tctacggagc acgagcggga cgaaggtcag acagtcaatg cacgtctggc
     1441 gtgggttcgt ccacccaatc ggtcgtttcg gcgagccgcc tcgcgacctc ctccgggtcg
     1501 aaccgcaccg gttcgccggg acgtcgcccg gccggatccg gccccaggat cgcattgaac
     1561 aagatcttct gggtgccctc ggaccctcca ccgtacgcag ggtcgaacga gaacacgatc
     1621 gagcggccgc gcccgacggg ttcgtcggcg accgcagtgg tgcccatcag ccagtgcgtg
     1681 ccccgacgga cgcccgacac ccggaacttc ccgctctccc gcgtggggaa gtggaccggc
     1741 gacacgccgt tgggcgcgaa catgccacgc gtatccatga gcggccacgc gctgcgaccg
     1801 acaccttcac cgagtggact gtcggcatcg atcagggcct tgaccatggg gccgtccata
     1861 ccgcccggca ggccttcgta ccgcgcacgg gagatcccca acgcgtaggc cagacgggcg
     1921 gcaccgtagc gccatcccac cagccggcca ccgcgattca cccagcgcac gagctcacgc
     1981 tggccggcct ccccgagctt gcgcaggcct tcgttgatac ctccggcggg caccaccagc
     2041 acgtcgatgc catcgagccc accggccacg tcttcggggt tgatctggga gtaaggcaga
     2101 ccccactgcg ttttgaagag ccaccgcgtg tgcccgatgt tctcgtacgc cgtgaagtca
     2161 cgcgacaggt gcaggatcgc gaccgagggc agcggcgagg ccagcgtcca cggcgcgggc
     2221 tcggcgacgg ggtccaccag gtcggcgtcg ggatccagcg tcagccccga catcgcgccg
     2281 tccaggttgt aggccagcgg caagctccag gagctgactc gatacgcctc attggtgggg
     2341 ttgaaggggt cttcgttcag ggccgcctgg atccatcgct tctggcgctg ggccatgggc
     2401 acccaatagg tgcctgccgg caacgtttcg cgatggggct cttccatgaa gggtcggaga
     2461 tcggagacct cgagcggcgc gttgagacgg tagacctcga cgtccatgcg ctggaggcgg
     2521 cggatcagca tgtccaactc gcggcgcttc gaagggtggt ccttgaagaa atagtgacgg
     2581 agcttccggg taggcacgtc tcgcaccggc cgagaatgcg gttcgtacgt gcggttgggc
     2641 tggagctggc ccttccgtcc ctcatccaca gcgtccacg
//
LOCUS       DATDBZ010000317         6442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2161073, whole genome shotgun sequence.
ACCESSION   DATDBZ010000317 DATDBZ010000000
VERSION     DATDBZ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161073"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VIX62_07655"
     CDS             complement(<1..1095)
                     /locus_tag="VIX62_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HEY5860142.1"
                     /translation="MTTGLRKRCLIFLAALAMTATWWGPARAAPGDLDPTFGGGGIVA
                     TRVTAGETSGAMAVAIQADGRIVAAGGTQGSDRERFAIVRYDVDGHLDGTFGHDGRVM
                     TGFDARRCVVADAVAIQSDGKIVAAGIQGCRGSFALARYLPNGKLDRSFGGDGKVVTR
                     FRSGKRYAESFAVTIQPDGKIVAAGLCCGARFFALARYLPNGKLDATFGADGKVTTDF
                     TPGSDVAYDVTLQSDGTILAAGVAAKDRDAARFALARYRPNGHLDTTFGADGKVTTRP
                     SDCVSEAHAVAVQSDGRIVAAGSAGCINEFALAGYMTDGSPDDTFGDGGVTVSDFTDF
                     GCAQADLEAMAIQSDGKLVATGLTACKGVSD"
     gene            complement(1100..2524)
                     /locus_tag="VIX62_07660"
     CDS             complement(1100..2524)
                     /locus_tag="VIX62_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY5860143.1"
                     /translation="MLTSPKNPKVIAASRLKKRAFRDEARRFLVEGAQAVSEALGVPG
                     ALETLFTDDELAPLAVQARQAGVEVHQVGDEVIRALTSTVTPQALVGVAPYLDRGLDA
                     LPEEGCVPILHEVRDPGNAGTVLRSADAAGAGGVVFTASSVDVYNPKTVRSSAGSLFH
                     LPVVRDAETTEAIAAVKDRGFRVLAMATGGADDLYRSDLSGPVAFVFGNEAHGLPADV
                     VALADDTVRVPQAGRAESLNLAAAATVCLFEWQRRRLREGEALENIIGAAAHDIRSPL
                     TAMKGFGYALEKRWDMMTEEQRAVMLQGIVHDADRMDTILRQLVDAARVVGGNLEIFP
                     EQVDVAELVLAIAENQRRDPDHPAVEWSGQAVTVFVDPARLKTTILAFMEVLVWWGRE
                     GSLLVEASLEDTFLHVRVSREASDLTTEDAESLFIPRRPGQGGGSKIGLFVARGVAES
                     QGGRAWGEVADGRLAFHAEIPVGF"
     gene            complement(2547..2909)
                     /gene="rplT"
                     /locus_tag="VIX62_07665"
     CDS             complement(2547..2909)
                     /gene="rplT"
                     /locus_tag="VIX62_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY5860144.1"
                     /translation="MARIKRSVHAKKKRREVLEKAKGYTGTRRKRYRAAKEQVMHSGN
                     YAYRDRRDRKAQFRRLWITRINAAVRPYGISYSVFINGLKAAGIEVDRKILADMAVND
                     PKAFAQLVDEAKAAQAAA"
     gene            complement(2914..3108)
                     /gene="rpmI"
                     /locus_tag="VIX62_07670"
     CDS             complement(2914..3108)
                     /gene="rpmI"
                     /locus_tag="VIX62_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY5860145.1"
                     /translation="MPKMKSHSATTKRFRVTRKGKVLHRKATGNHMLTKKSGGQRRRV
                     EGMSEVTAEKTKIKRLLGKA"
     gene            complement(3113..3778)
                     /gene="infC"
                     /locus_tag="VIX62_07675"
     CDS             complement(3113..3778)
                     /gene="infC"
                     /locus_tag="VIX62_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019884944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY5860146.1"
                     /translation="MGADGEQIGVVDTREALRQARDLDLDLVEVAPQADPPVCRIMDY
                     GKFKYERDVRQKEARKKQSRTGLKEIKFRPKIDPHDYATKKGHVVRFLNGGHKVKITI
                     MFRGREMAHTDLGRKILDRLVADLGEAIVVESFPKQEGRNMIMVIGPNKRYVEAQAKA
                     AAKLAKEQEEADGATDTAEPEASDTEPDAPAEAQPEPPAEPEPEPQPQPVAVESPASD
                     NEG"
     gene            complement(3969..4313)
                     /locus_tag="VIX62_07680"
     CDS             complement(3969..4313)
                     /locus_tag="VIX62_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5860147.1"
                     /translation="MDKPWGKVATYALNQPSSVRVITVEPEQETSVHYHRMRDEMWVV
                     LDAGLTIEIGNKVVEAQPGEEFMIPAEETHRIRCTAATRGRILEIAYGYTTEDDTQRL
                     EDAYGRSLEADW"
     gene            complement(4321..4758)
                     /gene="ribH"
                     /locus_tag="VIX62_07685"
     CDS             complement(4321..4758)
                     /gene="ribH"
                     /locus_tag="VIX62_07685"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY5860148.1"
                     /translation="MRVGVIAARFNEVVTGKLVEGAVEGLKKHGVDEERIDVAWVPGA
                     FELPLVAKRLARTERYDALICLGAVIRGETAHFELVANEAARGIAEVALDSGIPVIFE
                     VLATEDLAQAEARAGGAHGNKGWEAAEAALEMAWLMDALPEVR"
     gene            complement(4791..6074)
                     /locus_tag="VIX62_07690"
     CDS             complement(4791..6074)
                     /locus_tag="VIX62_07690"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY5860149.1"
                     /translation="MTELEIHEQDLDSEIFATIPEAVEDIRDGKIVLVVDDADRENEG
                     DFIMAADFATPEAVNFMVTHGRGIVCLPCAAWRLDELGIPQMVTDTTGGHEAAFTVSI
                     DYRQGTTTGTSAHDRAITAKAVTNTEVTPRDFQKPGHVFPLRAKDGGVLRRAGHTEAA
                     VDLATMAGLFPAGVICEVMNEDGTMARMPELIRVAREHGLKLISIADLIDHRRRREVL
                     VSKVAEATIPTPYGEFRSYAYESLVDGRTHIALVRGEMGDGIGVLTRVHSECLTGDVF
                     GSLRCDCGQQLESALAQVGEQGRGVILYVRGHEGRAIGLTHKLRAYELQDQGRDTVEA
                     NLELGFPADQREYGIGAQILVDLGVKTMRLLTNNPDKRAGLEGYGLSIEERIPLQIEP
                     TAENIGYLRTKQEKMGHLLDMTKAAPEPVAPEVQR"
     gene            complement(6071..>6442)
                     /locus_tag="VIX62_07695"
     CDS             complement(6071..>6442)
                     /locus_tag="VIX62_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY5860150.1"
                     /translation="EGHAVNLERPVTLAARLGGHIVQGHVDGVGEVQSVEPGEDGGSV
                     IRFHVPDNLLRYVIEKGSISIDGISLTVATLHPDGVSVAVIPHTLAVTTLGEAAPGDP
                     VNLEVDMIAKYLERLMEGRTA"
BASE COUNT          909 a         2274 c         2124 g         1135 t
ORIGIN      
        1 gtcactcacg cccttgcatg cggtgaggcc ggtggcgacg agcttcccat cggactggat
       61 cgccatcgcc tccaggtctg cctgagcgca cccgaagtcg gtgaagtccg agacggtgac
      121 gcctccatcg ccgaaggtgt cgtccgggga cccatccgtc atgtacccgg ccagggcgaa
      181 ctcgttgatg cacccggctg atccggcggc aacgatcctg ccgtcggact gcacggcgac
      241 cgcatgcgct tcggagacgc agtcggacgg tcgtgtcgtg accttcccgt cagcgccgaa
      301 ggtcgtgtcg aggtggccgt tcggtcggta gcgcgccagg gcgaaccgtg ccgcatctcg
      361 atccttcgcg gcgacaccgg cggccaggat cgtgccatcg gattggaggg tgacgtcgta
      421 cgcgacgtcg gaccctggtg tgaagtcggt cgtcaccttc ccgtcagcgc cgaaggtcgc
      481 gtcgagcttg ccgttcggaa gataccgagc cagagcgaag aaccgggcgc cgcaacacag
      541 ccctgccgcg acgatcttgc cgtcgggctg gatcgtcacc gcgaacgact cggcgtagcg
      601 cttcccgctg cggaacctcg tgacgacctt gccgtcaccg ccgaaggagc ggtcgagctt
      661 gccgttggga aggtagcgag ccagggcgaa cgagccgcgg cagccctgga tccctgcagc
      721 gacgatcttg ccgtctgact ggatggcaac ggcatcagca acgacgcatc gccgggcgtc
      781 gaagccggtc atcacgcgcc cgtcatgacc gaacgtgccg tcgagatgac cgtccacgtc
      841 gtagcggacg atcgcgaacc gttcccggtc ggagccctgc gtccctccgg cggccacgat
      901 ccttccatcg gcctggatcg ccacggccat ggcgccggac gtttctccgg cggtgacgcg
      961 cgtggccacg atccccccgc cgccgaaggt ggggtcgaga tctccgggcg ctgcccgggc
     1021 gggaccccac cacgtcgccg tcatcgcgag cgccgcgagg aagatgagac agcgcttgcg
     1081 caagcccgtc gtcacgacct cagaacccca cggggatctc ggcgtggaag gcgaggcgac
     1141 catcggcgac ctcgccccag gcccggcctc cctgcgactc ggcgacgccg cgcgccacga
     1201 acagcccgat cttgctcccc ccgccctggc cgggccggcg cgggatgaac aacgactcgg
     1261 cgtcctcggt cgtgaggtcg ctcgcttcgc gcgagacccg cacgtgcagg aacgtgtctt
     1321 cgagcgaagc ttccaccagg agggagccct cgcgtcccca ccagacgagc acctccatga
     1381 aggccaggat cgtggtcttg agccgggccg ggtcgacgaa cacggtgacg gcctggccgc
     1441 tccactccac cgcagggtga tcggggtcgc gacgctggtt ctcggcgatc gccagcacga
     1501 gctcggcgac gtcgacctgt tcggggaaga tctccaggtt ccctcccacg acgcgggcgg
     1561 catcgaccag ctggcgaagg atcgtgtcca tgcggtcggc gtcgtggacg atgccctgca
     1621 gcatcacggc gcgttgctcc tcggtcatca tgtcccaccg cttctcgagg gcgtagccga
     1681 agcccttcat ggcggtgagc ggcgagcgga tgtcgtgggc cgcggcgccg atgatgttct
     1741 cgagcgcctc cccctcgcgc agccggcggc gctgccactc gaacaggcac acggtcgctg
     1801 ccgcggcgag gttgagcgat tccgctcgcc ctgcctgcgg cacccgcacg gtgtcgtcgg
     1861 cgagcgccac gacgtcggcg ggcaacccgt gggcttcgtt gccgaacacg aacgccaccg
     1921 gtccggacag gtcggaccgg tagaggtcgt cggccccgcc ggtcgccatg gccagcacgc
     1981 ggaacccccg gtccttcacg gcggcgatcg cctcggtcgt ctcggcgtcc cggaccaccg
     2041 gcagatggaa cagcgacccg gccgacgagc gcacggtctt ggggttgtag acgtcgaccg
     2101 agctggcggt gaacaccacg ccgccggcac ccgccgcatc ggccgaccgg agcacggtgc
     2161 cggcgttccc cgggtcgcgc acctcgtgca ggatcgggac gcacccctcc tcggggagcg
     2221 catcgaggcc tcgatcgaga tacggagcga cccccaccag cgcctgcggc gtcacggtgg
     2281 aggtcagggc acggatcacc tcgtcaccca cctggtggac ctcgacgccc gcctgacggg
     2341 cctgcaccgc gagcggcgcg agttcgtcgt cggtgaacag cgtctcgagc gcgcccggca
     2401 cgccgagcgc ctcgctcacc gcctgggcgc cctcgaccag gaagcgacga gcctcgtcgc
     2461 ggaaggcgcg cttcttcagc cgggaggcgg cgatgacctt ggggttcttg ggggacgtca
     2521 gcacggagcc ctcccccgga cgcgtgtcag gctgcggctt gtgcggcctt ggcctcgtcg
     2581 accagttgcg cgaacgcctt gggatcgttg acggccatgt cggcgaggat cttgcgatcg
     2641 acctcgatgc cggcggcctt cagcccgttg atgaacacgg agtagctgat gccgtagggg
     2701 cgcaccgcgg cgttgatgcg ggtgatccac agccggcgga actgcgcctt gcggtcgcgg
     2761 cgatcgcggt aggcgtagtt gcccgagtgc atgacctgct ctttggcggc gcggtagcgc
     2821 ttgcgcctgg tcccggtgta gcccttggcc ttctcgagca cctcacggcg cttcttcttc
     2881 gcgtggacgg aacgtttgat tcgcgccatg gttctaggcc ttccccagca gtcgcttgat
     2941 cttcgtcttc tccgccgtga cctcggacat gccctcgacg cgccggcgct ggccgccgct
     3001 cttcttcgtg agcatgtggt tgccggtggc cttgcggtgc agcaccttgc ccttgcgcgt
     3061 cacgcggaag cgcttggtgg tggcgctgtg gctcttcatc ttcggcatcg atctatcctt
     3121 cgttgtcgga tgcggggctc tccacggcca ccggctgcgg ctgaggctcc ggttcgggct
     3181 cggccggcgg ttccggctgg gcttcggctg gagcgtcagg ctcggtgtcc gatgcttcgg
     3241 gttcggcggt gtcggtcgcg ccgtcggcct cttcctgttc cttggccagc ttcgcggccg
     3301 ccttggcctg tgcctcgacg tagcgcttgt tgggcccgat caccatgatc atgttccggc
     3361 cttcctgctt ggggaaggac tccaccacga tggcttcccc gagatcggcg accaagcggt
     3421 ccaggatctt gcgaccgagg tccgtgtgcg ccatctcgcg cccgcggaac atgatcgtga
     3481 tcttgacctt gtgccccccg ttcaggaacc ggacgacgtg ccccttcttg gtggcgtagt
     3541 cgtgcgggtc gatcttgggg cggaacttga tctccttcag gccggtccgc gactgcttct
     3601 tgcgggcctc cttctgccga acgtcgcgtt cgtacttgaa cttgccgtag tccatgatcc
     3661 gacacacggg cggatcggct tgcggtgcga cttcgaccag gtccagatcc aggtcacggg
     3721 cttggcggag cgcctcgcgg gtgtcgacca ccccgatctg ctcgccgtct gcccccacca
     3781 ggcgaacctg gggagaccgg atccggtcat ttatgcgcgg atcggtcgag accccgcatc
     3841 acctccacag atgaacgagc accgggtgtt gtcggcccgg tgcatggtga acacacgtgc
     3901 ttgctcttct gggcaaggag tataggggtc gcggccgacg gctgtctcgc acgcccgata
     3961 cggctggctc accagtccgc ctcgagcgat cggccgtaag cgtcttcgag gcgctgcgtg
     4021 tcatcctcgg tcgtgtagcc gtacgcgatc tccaggatcc ggccgcgcgt tgctgccgtg
     4081 caacggatcc gatgggtctc ctcggccggg atcatgaact cttcacccgg ctgagcctcc
     4141 accaccttgt tgccgatctc gatcgtgagc cccgcgtcca gcacgaccca catctcgtca
     4201 cgcatgcggt ggtagtgcac gctggtctcc tgctccggtt cgactgtgat gacccgcacc
     4261 gacgacggct ggttcagggc gtaggtcgcg accttgcccc acggcttatc caccaacacc
     4321 tcatctcacc tccgggagcg catccatgag ccaggccatc tcgagcgcgg cctcggccgc
     4381 ctcccacccc ttgttgccgt gcgcgccacc agcacgcgcc tcggcctggg cgagatcctc
     4441 ggtggcgagc acttcgaaga tcaccgggat gcccgaatcc aacgccacct cggcgatgcc
     4501 acgtgcggct tcgttggcga cgagctcgaa gtgcgcggtc tccccccgga tcaccgcacc
     4561 caggcagatc agcgcgtcgt agcgctccgt cctcgccagg cgcttggcca ccaacggcag
     4621 ctcgaacgca cctggtaccc acgccacgtc gatccgctcc tcgtccacgc cgtgcttctt
     4681 gaggccctcg acggcgccct cgaccagctt gcccgtgacc acctcgttga agcgcgccgc
     4741 gatcaccccg acgcgcatcc ccgagccgtc gtggaccccg acgaacgtcc tcaccgctgg
     4801 acctcgggcg ccacgggctc aggcgccgcc ttggtcatgt cgagcagatg acccatcttc
     4861 tcctgcttcg tgcgcaggta gccgatgttc tcggcggtgg gttcgatctg gagcgggatg
     4921 cgttcctcga tcgacaaccc gtagccctcg agcccggccc gtttgtccgg gttgttcgtg
     4981 agcagtcgca tcgtcttgac gccgaggtcc accaggatct gtgcgccgat gccgtactcg
     5041 cgctgatcgg cggggaagcc gagttccagg ttggcctcga cggtgtcacg accctggtcc
     5101 tgcagctcgt aggcgcggag cttgtgcgtg agtccgatcg cgcggccttc atgaccgcgt
     5161 acgtacagga tcaccccgcg gccctgctcc cccacctggg ccagcgcgct ctcgagctgc
     5221 tgtccgcagt cgcagcgcaa cgagccgaac acgtcgccgg tgaggcattc ggagtgcacc
     5281 cgcgtgagga cgccgatgcc gtcacccatc tcgccccgga ccagcgcgat gtgcgtgcgt
     5341 ccgtcgacca ggctctcgta cgcgtaggaa cggaactcgc cgtacggcgt ggggatcgtg
     5401 gcctcagcca ccttcgacac cagcacctcg cggcgtcgcc gatggtcgat caggtcggcg
     5461 atcgagatca gcttcaggcc gtgctcgcgc gcgacacgga tcagctcggg catgcgcgcc
     5521 atcgtgccgt cctcgttcat cacctcgcag atcacgcccg cggggaagag gccggccatc
     5581 gtggcgaggt cgaccgcggc ttcggtgtgt cccgcgcggc ggagcacgcc gccgtccttg
     5641 gcccgcaacg ggaagacgtg gcccggcttc tggaagtcgc gcggcgtgac ctccgtgttg
     5701 gtgacggcct tcgccgtgat cgcccggtcg tgcgcgctgg ttcccgtggt ggtgccctgg
     5761 cgatagtcga tcgagaccgt gaaggccgcc tcgtgcccgc ccgtggtgtc ggtgaccatc
     5821 tgcgggatgc ccagctcgtc gaggcgccat gcggcgcacg gcaggcacac gatgccccgg
     5881 ccgtgggtca ccatgaagtt caccgcctcg ggcgtggcga agtccgccgc catgatgaag
     5941 tcgccttcgt tctcccggtc ggcgtcgtcg accaccagga cgatcttgcc gtcccggatg
     6001 tcttcgacgg cttcagggat cgtcgcgaag atctcgctgt ccaggtcctg ctcgtggatc
     6061 tccaactcgg tcatgccgtc cgtccttcca tcaggcgttc gaggtacttc gcgatcatgt
     6121 cgacctccag gttcaccggg tcgccgggcg cggcttcacc cagcgtggtg acggccaggg
     6181 tgtgcgggat caccgcgacg ctgacaccgt cgggatgcag ggtcgccacg gtcaagctga
     6241 tgccgtcgat cgagatcgag cccttctcga tcacgtatcg cagcaggttg tcggggacgt
     6301 ggaaccggat cacggaaccg ccgtcctcac cgggctccac gctctgcacc tcgccgacac
     6361 cgtcgacgtg gccttgcacg atgtgtccgc ccaggcgcgc cgccagggtg acgggacgct
     6421 ccaggttcac ggcgtgccct tc
//
LOCUS       DATDBZ010000318         9278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2962529, whole genome shotgun sequence.
ACCESSION   DATDBZ010000318 DATDBZ010000000
VERSION     DATDBZ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2962529"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1011
                     /locus_tag="VIX62_07700"
     CDS             19..1011
                     /locus_tag="VIX62_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5860151.1"
                     /translation="MSDVLTLEDLRITYHTASGGIPAVRGVNLNVKKGEVVGLAGESG
                     CGKSTIAGAVLRLLPAKTKIEGTLLLEGEDVLSMKPGRLRAVRWTGASIVFQGAMHAL
                     NPVKRVGDQVAEAITVHNQAGEKEARVRVGALLEQVGLPPRRMSDFPHELSGGQKQRV
                     MIAMALACSPSLIIADEPTTALDVMVQAQVLKLMKELQRDLGLSMIFITHDLSVLVET
                     SDRLAIMYAGKIVEEGPANTVFHDPKHPYTEALAASFPEIGDTRFRGNPTGLGGDPPD
                     PADVPSGCSFHPRCPQAFEDCSSIEPELYFAGEGRRAACLLVSGAKAGVTEATT"
     gene            1008..2087
                     /locus_tag="VIX62_07705"
     CDS             1008..2087
                     /locus_tag="VIX62_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5860152.1"
                     /translation="MSTETDVNLPQPPSPGDGPVIEVDDLHVTFAGRVGVFEGLRGKK
                     ATQSRAVDGVSFQLRRGEVLALAGESGCGKTTTARAIMGLQPPNAGVISFKGKPLPRN
                     LKPYRRKVQMVFQDPTGSLNPRQTIYEIIAEALRIHGIDRGSEGETEERLVAKALSRA
                     GLRPPERFFLRYPHELSGGQRQRVVIAGALVLEPEVIVADEPVSNLDASVRGEILQLL
                     MKLRAELGLTIIIVTHDLGLAWTVADRVAIMYLGRIVEIGPAEDVLLNPFHPYTKALL
                     DVVPEAGGLERPILAGEPPDPTRIPPGCRFHPRCPVVASGKAAELGIEARCKGEDLAL
                     EMLAPEHEAACYQASLEDTRPAPTS"
     gene            complement(2084..2899)
                     /locus_tag="VIX62_07710"
     CDS             complement(2084..2899)
                     /locus_tag="VIX62_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860153.1"
                     /translation="MKPSTRRSLLIVFGGLLVSGLLLTLLRGDARGARDIALAIFGLA
                     IMAGFALMAWWVRTQPRLDAARAAARDLGLGYAAKDEFGLIDLPFPLLQRPATVRGLG
                     SVMFGTWRDREVKIFEYWYARSSNQALDDFERFSCLVSSLPAWWPDLVIVPETIVTRA
                     ADHLTMNEVNLESDAFNRAFSVRSQDQRFANALLDARMMAWLLDRAGPWGFEIAGGML
                     LCYRTPMLQPWEVRPLLETSAEFMDMVPGVVSSLFEPEAAGRVPQRPDRSPGR"
     gene            complement(2959..3609)
                     /locus_tag="VIX62_07715"
     CDS             complement(2959..3609)
                     /locus_tag="VIX62_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860154.1"
                     /translation="MNEQSLQRVNAFLAAASQRTEVGQVLDVTPAEIGRELGFPDALS
                     TARAVRALIARKRLEPAQGSYRLLDARPVDASEKEAVGRKPRKTRTAKAARGTASGSR
                     AATGGAAYSDFGRAMVDRLVELGREVAELRTNLRSAREESRGARQDRDEAEARARSLA
                     EKSRELEARAEMAESNLRTLLATVRTKETPRDAPVSDSEMEAILGVLKQGDGSQPD"
     gene            3689..4849
                     /locus_tag="VIX62_07720"
     CDS             3689..4849
                     /locus_tag="VIX62_07720"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5860155.1"
                     /translation="MGDASDGHGPAPLAGIRVLDLSRVLAGPLATMTLGDLGADVIKV
                     ERPGEGDDTRHWGPPFSGDDAAYFLSLNRNKRSVALDLKTRDGVAEVRRLAREVDVLI
                     ENFRPGLMGELGLALEDLRRDHPPLVTCSLTAFGDDAAATATRPGYDIIVQALSGLMS
                     VTGERDGEPTKAGVALLDVITGLQATIGIQAALLERARTGRGRHVSVSLFDASVAAMV
                     NQASNYLVGDQVPKAMGSQHPNIVPYQVFHGSDRPFIVAGGNDRLFRRICEVLDHPEW
                     AEDERFVTNEARVSHRDALISMMVEVFATRTAGEWLAALEDAAVPCAPIRTLDEVFAS
                     PEGAALIDTVADPAREGTLRTVRNPLRFDGQALAVRRPPPTLGEHTDEVLDG"
     gene            4846..5691
                     /locus_tag="VIX62_07725"
     CDS             4846..5691
                     /locus_tag="VIX62_07725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5860156.1"
                     /translation="MNAADRWAEALAARAIPRSIIDAAPTSPWGFPTELFRTRARTSS
                     ARPVATPTTVRALEALPAPGTVLDVGVGGGATSLPLAPRAGRITGFDQQADMLEVFLE
                     AAGAAGVDARAVQGRWPADEATAPPADVVVSGHTVYNAPDLEPFVSALDTHAGRRVVL
                     EATERHPLGWMHDLWRRFHDLEIPDAPSIDLAVEVIEATGIVVSREDRPDRDDDPGAG
                     GFDSAEEAVALIRTRLCLPASQDEELTEALGNRLRLRHGLWTAGPPSRTVVTLWWDAH
                     HDEPS"
     gene            complement(5686..6276)
                     /locus_tag="VIX62_07730"
     CDS             complement(5686..6276)
                     /locus_tag="VIX62_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860157.1"
                     /translation="MPKAVKILLWIGAFLACAGVGAYVAAHTDPFPPGVDDPGANAVG
                     PVSTEVPSGGIVVLIDTRTSHDLYVGGRCAISWRIELGLGAADGTVDGSGVATAKSEL
                     RCDEPTAQVQADELQLSATGTIADGELHFRVDETGRSPVGAQELTGFAKTIPTLRFEL
                     PAREGATASFDVDVPDRDRGTYGAIGTVTVSSVPAS"
     gene            complement(6286..8220)
                     /locus_tag="VIX62_07735"
     CDS             complement(6286..8220)
                     /locus_tag="VIX62_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860158.1"
                     /translation="MRFRYSRWDGTQDPFGPDIAAADVLEQLSDEILMGGSADAALQR
                     LLRRGMQGRFSGLDALKRRLRQRREEEQEMLNLAGPLEQIRERLDEILERERTELTFR
                     DDDDARMRESFLDSLPPDAPGQVAELKEYRFSDAGAQQMFDALMQELREQVMGAHFRQ
                     MAEGMQSLTPEDIARFKDMLAELNGLIEMRDRGEDTQPAFEDFMTRHGDLFPDNPRTL
                     DELLEHMAKRMAAMSRFMASLTPEQREELMGLSQQIMQDMDLAFEMDRLGSNLANAFP
                     DMPWGDPVMGEGEGSMPMSAAVDVMERLHDYEDLDRSMQGDYAGAALDDVDEDALRRT
                     LGDPAVQDLRRLKQIERALEKAGLVQRRRGRLEVTPRGARRLGEKALTQIFDELKRDR
                     EGTHEARDAGGLAEPTGATRPWNFGDHGQIAVGKTVFNAVVRSEPGQPVRLRPDDFEI
                     VEAEQRTEAATALLLDLSFSMPLRGHFIHAKKMALALHALIEGRYPHDTLYLIGFSDY
                     ARQLQPEDLTAPGFERVYGTNMQHAFMLANRLLSQHPRATRQVIMVTDGEPTAHLEDD
                     QAYFNWPPVPETIEKTLTEAIRLSKAGVRLNIFMLEESEGLARFMERLARVTSGRVFL
                     MDDHRVGEFVLRDYVARRSR"
     gene            complement(8272..8784)
                     /locus_tag="VIX62_07740"
     CDS             complement(8272..8784)
                     /locus_tag="VIX62_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860159.1"
                     /translation="MPLRSKGEDAREGKARADLATSLRRLDAYSFFIVPADSAFGGDV
                     IVVGTTGAFLIKACGLSGVAVIKGRRPLVGDEAIDGLRALRAGAKRLSSTLSAKSALA
                     EVEAIVCLTEAIAPTVAEAAGVRFVRVADIARELSARPGVQSHTRAQSAARALGVQVA
                     GDQHRHFAVR"
     gene            complement(8835..9224)
                     /locus_tag="VIX62_07745"
     CDS             complement(8835..9224)
                     /locus_tag="VIX62_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860160.1"
                     /translation="MSDVHEVVGFLVVGVFAVGWIWGLGAWIARRGPGDRYWTWLTVA
                     QVVAGLQAVLGIILLLFGKRPSTWLHLVYGFGPFVILAIAHALARDLQKTQPGKKPIP
                     AWVPFAFGAFICFGLTLRALMTGLGVG"
BASE COUNT         1454 a         3307 c         3125 g         1392 t
ORIGIN      
        1 tccgaaactg agggaccgat gagtgatgtc ctgaccctgg aagacctgcg gatcacctat
       61 cacacggcct ccggcggcat cccggccgta cgcggggtga acctgaacgt gaagaagggc
      121 gaggtcgtcg gcctggccgg agaatccggc tgcggcaagt cgacgatcgc gggcgccgtg
      181 ctgcggctgc tccctgccaa gacgaagatc gaaggcacct tgctgctcga aggcgaggac
      241 gtcctgtcga tgaagcccgg ccggctgcgc gccgttcgat ggaccggtgc ctcgatcgtc
      301 ttccagggcg cgatgcacgc gctcaacccg gtgaagcggg tcggcgacca ggtggccgag
      361 gccatcaccg tgcacaacca ggccggcgag aaggaagcgc gcgtgcgcgt cggcgcgctc
      421 ctggagcagg tcgggttgcc gccgcgccgc atgagcgact tcccgcacga actctccggt
      481 ggtcagaagc aacgcgtgat gatcgccatg gcgctggcgt gctcgccgtc gctgatcatc
      541 gccgacgaac ccaccaccgc gctcgacgtc atggtccagg cgcaggtcct gaagctcatg
      601 aaagagctgc agcgcgacct cggcctgtcg atgatcttca tcacccacga cctgtcggtc
      661 ctggtggaga cctcggaccg gctcgccatc atgtacgcgg gcaagatcgt ggaggagggg
      721 cccgccaaca cggtgttcca cgatccgaag cacccgtaca ccgaggccct cgcggcctcg
      781 ttcccggaga tcggcgacac gaggttccgg ggcaatccga ccggcctcgg cggcgaccct
      841 cccgatccgg cggacgtgcc gagcgggtgt tccttccacc cgaggtgtcc gcaggccttc
      901 gaggactgct cctcgatcga gcccgagttg tatttcgccg gcgagggccg gcgagcggcc
      961 tgcctgttgg tgtcgggtgc gaaggccggc gtcacggagg ccacgacatg agcaccgaga
     1021 ccgacgtcaa ccttccgcag ccacccagcc ccggggatgg gcccgtgatc gaggtcgacg
     1081 acctgcacgt caccttcgcc ggacgcgtgg gggtcttcga agggctgcgc ggcaagaagg
     1141 cgacccagtc acgcgccgtc gacggcgtca gcttccagct ccgcaggggt gaggtgctgg
     1201 ccctggccgg cgagtccggc tgcggcaaga ccacgaccgc tcgcgccatc atgggcctgc
     1261 agccgccgaa cgccggcgtg atctcgttca agggcaaacc gctgcccagg aacctgaagc
     1321 cgtatcggcg gaaggtccag atggtgttcc aggacccgac cggcagcctc aacccccgcc
     1381 agacgatcta cgagatcatc gccgaggctc tgcggatcca cggcatcgac cgcgggagcg
     1441 agggggagac cgaggagcgg ctggtggcca aggcgctgtc gcgcgccggc ctgcgaccgc
     1501 ccgaacgctt cttcctgcga tacccgcacg agctctccgg tggccagcgt cagcgcgtgg
     1561 tgatcgcggg ggccctcgtc ctcgagcccg aagtcatcgt cgccgacgaa cccgtgtcca
     1621 acctggatgc cagtgtgcgc ggcgagatcc tgcaactcct gatgaagctc cgcgccgaac
     1681 tcggcctcac gatcatcatc gtgacccacg acctgggcct cgcctggacg gtggccgacc
     1741 gggtggcgat catgtacctg gggcgcatcg tggagatcgg ccccgccgag gacgtgctcc
     1801 tgaacccctt ccatccgtac acgaaggcgt tgctcgacgt cgtgccggag gccggcggtc
     1861 tggagcgccc catcctggcg ggcgagccgc ccgaccccac gcgcatccca cccgggtgcc
     1921 gcttccatcc ccgttgcccc gtggtggctt cgggcaaggc ggccgagctc gggatcgagg
     1981 cgcgctgcaa gggtgaggac ctggcgctgg agatgctggc gcccgaacac gaagcggcgt
     2041 gctatcaggc gtccctcgaa gacacgcgcc cggcgccgac gtcctagcgg ccgggcgatc
     2101 gatcgggacg ctgaggaacc cgacccgcgg cctcaggctc gaacaggctc gagacgacgc
     2161 cgggaaccat gtccatgaac tccgcggatg tttccagcag gggtcgcacc tcccacggct
     2221 ggagcatggg cgtccggtag cacagcaaca tcccgccggc gatctcgaac ccccatggtc
     2281 ccgcccggtc cagcagccac gccatcatcc gtgcgtcgag gagcgcgttc gcgaatcgct
     2341 gatcctgcga ccggaccgag aacgcccggt tgaaggcatc ggactccagg ttcacctcgt
     2401 tcatcgtgag gtgatccgcc gctcgggtga cgatcgtctc cgggacgatc accagatccg
     2461 gccaccacgc cggcagggag ctcaccaggc acgagaaccg ttcgaagtca tcgagggctt
     2521 ggttcgagga gcgtgcgtac cagtactcga agatcttgac ctcacgatcc cgccaggttc
     2581 cgaacatcac ggatccgagt cctcgcacgg tggcaggacg ctgcaggagc gggaacggca
     2641 gatcgatcag tccgaactcg tccttcgctg cgtacccgag ccccaggtcc cgggcggccg
     2701 ctcgggcggc gtccagtcga ggctgcgttc gcacccacca tgccatgagc gcgaagccgg
     2761 ccatgatcgc caggccgaag atcgcgagcg cgatgtcgcg ggctccccgc gcatcgcctc
     2821 gcaggagcgt caggagcaac ccggacacga gcaggccgcc gaacacgatg agcagcgagc
     2881 gccgggtgga gggcttcatg ctccacgtat cggcaggcgg ggcgacgctg ttgatccggg
     2941 agcatccagg cggggtgctc agtccggctg ggatccatcc ccttgcttga ggaccccgag
     3001 gatcgcttcc atctccgaat ccgacaccgg cgcgtcgcgc ggcgtttcct tggtccgcac
     3061 ggtggcgagc agggtgcgca ggttcgactc ggccatctcc gctcgggctt cgagctcgcg
     3121 gctcttctcg gcgagactgc gggcgcgagc ttcggcttcg tcgcgatcct gacgcgcgcc
     3181 ccgcgactcc tcccgcgcgc tccgcaggtt ggtgcggagc tcagcgacct cgcgaccgag
     3241 ctcgaccagg cggtcgacca tcgcccggcc gaaatcggaa tacgcggccc cacccgtggc
     3301 ggcgcgcgac cctgacgcgg tgcctcgcgc tgccttcgcg gtgcgtgtct tgcgaggctt
     3361 gcgccccacg gcctccttct cggacgcatc caccggccgg gcgtccaaca ggcggtagga
     3421 gccctgcgcg ggttcgaggc gcttgcgcgc gatcagggcc cggacggcgc gcgccgtgga
     3481 caacgcgtcg gggaacccca gctcccggcc gatctcggcc ggggtgacat cgagcacctg
     3541 acccacctcc gtcctctggg aggctgcggc caggaatgca ttcacccgct gcaggctctg
     3601 ctcgttcatg gcgccatcat gcccgacgtc ggtgccgaaa ggccagtgcg cctcagcgga
     3661 acgccgggcg acggtaggct ggcccaccat gggtgatgcg tcggatgggc acgggcccgc
     3721 tccactcgcc gggatccgcg ttctggatct gtcgagggtg ctggcgggtc cgctggcgac
     3781 gatgacgctg ggcgacctcg gtgccgacgt gatcaaggtc gaacggcccg gcgaaggcga
     3841 cgacacgcgc cactggggcc ccccgttctc aggcgatgac gccgcctact tcttgtccct
     3901 caaccggaac aagcgctcgg tagccctcga cctcaagacc cgggacgggg tcgccgaggt
     3961 tcggaggctc gcccgtgagg tcgacgtcct gatcgagaac ttccgccccg gcctcatggg
     4021 cgagctgggg ctggcgctcg aggacctccg ccgggaccat ccaccgctgg tcacctgctc
     4081 gctcaccgct ttcggcgacg acgcggccgc aacggcgacc cgcccgggct acgacatcat
     4141 cgtccaggcg ctgagcggcc tgatgagcgt caccggcgaa cgcgacggcg aacccacgaa
     4201 agccggcgtc gcgctgctcg acgtcatcac cggactccag gccacgatcg ggatccaggc
     4261 cgcgctgctc gaacgcgcgc gaacggggcg aggacggcac gtcagcgtgt cgctcttcga
     4321 tgccagcgtc gccgccatgg tgaatcaagc ctcgaactac ctggtgggcg accaggtgcc
     4381 gaaggccatg ggctcccagc accccaacat cgtgccgtac caggtgttcc acgggtcgga
     4441 ccggccgttc atcgtggccg gcggcaacga tcgattgttc cggaggatct gcgaggtgtt
     4501 ggatcatccc gagtgggccg aggacgagcg tttcgtcacg aacgaggcgc gcgtgagcca
     4561 ccgcgacgcg ctgatctcca tgatggtcga ggtgttcgcc actcggaccg ccggggaatg
     4621 gctcgctgcc ctcgaggacg ccgccgtccc gtgcgccccg atccgaaccc tggacgaggt
     4681 cttcgccagt cccgaggggg ccgcgctgat cgacaccgtt gcggaccccg cccgagaggg
     4741 gacgctccgc accgtccgca acccgctccg cttcgacggc caggcgctcg ccgtgcgtcg
     4801 gccaccgccc acgctgggcg agcacacgga tgaggtcctg gacggatgaa cgccgccgat
     4861 cggtgggccg aagcgctggc ggctcgcgcg atcccccggt cgatcatcga cgcagccccc
     4921 acgtctccct ggggcttccc caccgagctg ttccgcacgc gcgcccggac gtcgtcggca
     4981 cgaccggtcg cgacgccgac gacggttcgg gcgctggaag cgctgccggc cccgggcacc
     5041 gtcctcgacg tcggcgtggg cggcggcgcc acatcgctgc ccctcgcccc gcgcgccggt
     5101 cgcatcaccg ggttcgatca gcaggcggac atgctggagg tcttcctcga ggccgccggc
     5161 gccgccgggg tcgatgccag ggcggtgcag ggccgttggc ccgccgacga agcgacggcg
     5221 ccgccggccg acgtcgtggt ctccgggcac acggtgtaca acgcacccga cctcgaaccc
     5281 ttcgtctcgg ccctcgatac gcacgccgga cggcgggtcg tgctcgaagc caccgagcgc
     5341 catccgctgg gatggatgca cgacctgtgg cgaaggttcc acgatctcga aatccccgac
     5401 gcgccttcga tcgatctggc cgtggaggtg atcgaggcca ccgggatcgt ggtgtcgcgc
     5461 gaggaccggc ccgatcgcga tgacgacccg ggggccggcg gcttcgactc ggccgaggag
     5521 gccgtcgcct tgatccgcac tcggctttgc ctgccggcgt cgcaggacga ggaactcacc
     5581 gaagcgttgg gcaaccgttt acggctccgt cacgggctgt ggaccgccgg cccgccctcc
     5641 cgcaccgtgg tcaccctctg gtgggacgcc caccacgacg agccgtcatg acgcgggcac
     5701 ggagctgacc gtcaccgtgc cgatcgctcc ataggtcccc cggtcacggt cggggacatc
     5761 cacgtcgaag ctcgcggtcg cgccctcgcg cgccggcagc tcgaagcgca gcgtggggat
     5821 cgtcttcgcg aagccggtca gctcctgggc gccgaccggc gaccgaccgg tttcgtccac
     5881 gcggaagtgc agctcgccgt ccgcgatcgt cccggtggcg gagagctgga gttcgtcggc
     5941 ctgcacctgc gcggtgggtt cgtcacagcg gagctcgctc ttggcggtgg cgacgcccga
     6001 gccgtcgacc gtaccgtcgg cggcgcccag ccccaactcg atccgccagc tgatcgcgca
     6061 ccggccaccg acatagaggt cgtgggacgt tcgggtgtcg atcagcacga cgatgcctcc
     6121 gctcgggacc tcggtcgaga ccggtccgac agcgttggcg cccgggtcgt ccaccccggg
     6181 cgggaacggg tcggtgtgcg ccgccacgta ggcacccacg ccggcgcacg ccaggaacgc
     6241 gccgatccac agcaggatct tgaccgcctt gggcatcggc tgaccctacc gcgagcgacg
     6301 ggccacgtag tcgcgcagga cgaactcccc cacgcgatgg tcatccatca ggaagacgcg
     6361 tccggacgtc acccgggcga gccgctccat gaaccgcgcg agcccttcgg attcttcgag
     6421 catgaagatg ttgagccgca cgccggcctt cgacagacgg atcgcctcgg tgagggtctt
     6481 ctcgatcgtc tcgggcacgg gcggccagtt gaagtaggcc tgatcgtcct ccaggtgcgc
     6541 ggtgggctcg ccgtcggtga ccatgatcac ctggcgcgtc gcccgcggat gctgcgacag
     6601 taggcggttg gccagcatga aggcgtgttg catgttcgtg ccgtacacac gctcgaatcc
     6661 tggcgcggtg aggtcctcgg gctgcagctg acgcgcgtag tcgctgaacc cgatcaggta
     6721 cagggtgtcg tgcggatacc ggccctcgat cagcgcatgc agcgccagcg ccatcttctt
     6781 ggcgtggatg aagtgcccac gcagcggcat cgagaacgac aggtccagca ggagggcggt
     6841 ggcggcttcg gtgcgctgtt cggcctccac gatctcgaag tcgtcgggtc gcagccggac
     6901 gggctgaccg ggctcggaac ggaccaccgc gttgaacacc gtcttgccca ccgcgatctg
     6961 tccgtggtcg ccgaagttcc acgggcgcgt ggccccggtg ggctcggcga ggccacccgc
     7021 gtcgcgggcc tcgtgcgtgc cttcgcggtc acgcttgagc tcgtcgaaga tctgcgtcag
     7081 cgctttctca cccagccggc gggcgccccg tggcgtgacc tccaaccggc cacgacgccg
     7141 ctgcaccaat cccgccttct ccagtgcgcg ttcgatctgc ttgagccggc gaaggtcctg
     7201 gaccgccgga tcgcccaggg tgcggcggag ggcatcttcg tccacgtcgt cgagcgcggc
     7261 gcccgcgtag tccccctgca tcgaacgatc caggtcttcg tagtcgtgca gacgctccat
     7321 cacgtcgacg gcggccgaca tcggcatcga accctcgcct tcacccatga ccgggtcacc
     7381 ccacggcatg tccgggaagg cgttcgcgag gttggacccc aggcggtcca tctcgaaggc
     7441 gaggtccatg tcctgcatga tctgctggga caaccccatg agctcctcac gctgctcagg
     7501 cgtgagcgag gccatgaacc ggctcatcgc ggccatccgt ttggccatgt gttcgagcag
     7561 ttcgtccagc gtcctcgggt tgtcggggaa caggtcgccg tgccgggtca tgaaatcctc
     7621 gaacgcgggc tgggtgtcct cgccacggtc gcgcatctcg atcaggccgt tcaactcggc
     7681 cagcatgtcc ttgaaccggg cgatgtcctc cggcgtgagg ctctgcatcc cctcggccat
     7741 ctgccggaaa tgggcaccca tcacctgttc gcgcagttcc tgcatcagcg catcgaacat
     7801 ctgctgcgca cccgcatcgg agaagcgata ctccttcagc tcggccacct ggcccggcgc
     7861 atcgggcggc agcgagtcga ggaacgactc ccgcatccgg gcatcgtcgt cgtcgcggaa
     7921 ggtcaactcg gtgcgttccc gctcgaggat ctcgtcgagc cgctcgcgga tctgctccag
     7981 cggcccggcg aggttgagca tctcctgctc ttcctcccga cgctgacgga ggcgccgctt
     8041 caacgcatcc agtcccgaga accggccctg catgcctcgc cgcaacagcc gctggagggc
     8101 cgcgtcggcg ctcccgccca tgaggatctc gtcgctcagc tgctcgagga catcggccgc
     8161 cgcgatgtcg ggcccgaagg ggtcctgggt gccgtcccag cgcgagtacc ggaatcgcat
     8221 acgcacccag cctacggcgg gtcgcgcgga aggtgggggg tgcccgctac ctcagcgaac
     8281 cgcgaaatga cggtgctgat cgcccgcgac ctgcacgccc agcgctcgtg cggcgctctg
     8341 ggccctggtg tgggactgca cgccaggacg agccgacagc tcgcgcgcga tgtcggcaac
     8401 acggacgaac cgaaccccgg cggcctcggc caccgtgggc gcgatcgctt cggtcaggca
     8461 cacgatcgct tcgacctctg ccagcgccga cttcgcgctc aaggtggagc tcaatcgctt
     8521 ggcccccgcg cgcagggctc gcaagccgtc gatggcctcg tcgcccacca agggccggcg
     8581 tcccttgatg accgccacgc ctgagaggcc gcatgccttg atcaggaagg caccggtggt
     8641 gccgacgacg atgacgtcac caccgaacgc actgtcggcg ggcacgatga agaagctgta
     8701 cgcgtcgaga cgacgcagcg aggtcgcgag gtcggcgcga gccttcccct cccgcgcgtc
     8761 ctcgcccttg gatcggagcg gcatggttca cggtgtcgac cggttcctcc ggcatcttga
     8821 gccggacgac cccatcaccc cacgccgagc cccgtcatca gcgcccgaag cgtcagcccg
     8881 aagcagatga aggctccgaa ggcgaacggc acccacgcgg ggatcggctt cttgccgggc
     8941 tgggtcttct gcaggtcacg cgccagcgca tgcgcgatcg cgaggatgac gaacgggccg
     9001 aatccgtaca cgaggtggag ccaggtggac ggacgcttgc cgaacagcag caggatgatg
     9061 ccgagcaccg cttggagccc ggccacgacc tgggccacgg tcaaccaggt ccagtaccga
     9121 tcacccgggc ctcgacgagc gatccatgcg cccaagcccc agatccagcc cacggcgaac
     9181 acgccgacca ccaagaaccc cacgacctcg tgcacatcgg acacggccgg atactacgag
     9241 gcgtcggcac ccgtgtagcg cgccgcgccg tcgccccc
//
LOCUS       DATDBZ010000319         3074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_320105, whole genome shotgun sequence.
ACCESSION   DATDBZ010000319 DATDBZ010000000
VERSION     DATDBZ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320105"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VIX62_07750"
     CDS             complement(<1..767)
                     /locus_tag="VIX62_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY5860161.1"
                     /translation="MPEHDDRRGGPWRGPDWDGRPPWAGRGRPGFDAGRRFRRGAIAA
                     LVLVVLLVAALATVVASAFGGNAPHPVVTVPLALAVIAALIASARWLWRNTRAIGTLM
                     DAADRVAGGDYTTRVEVTGSRQLGRLTSSFNQMTERLETDEARRRELLADVAHELRTP
                     LQVIRGTVEGMLDGLYPADAERLRPLVEETVVMDRLLEDLRTLSMAEAGVLELHRETV
                     DPRALARDVATAFRTMADEAGITLTIATDAGTDGTIEA"
     gene            complement(760..1440)
                     /locus_tag="VIX62_07755"
     CDS             complement(760..1440)
                     /locus_tag="VIX62_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860162.1"
                     /translation="MQRILVVEDEMQIARTLRDYLEVAGFDVTVVGDGSAALASARGD
                     RPDLVVLDLGLPGMDGLDVARELRRTSATPIVMLTARGEESDRIVGLELGADDYLVKP
                     FSPKELVARVRAVLRRTNGTTAGAEVMRAGGVEVDLPKMRARVDGRAVDLTPTEFQLL
                     ATFVREPGRVFTRGQLLDALHGVTVDTYERAIDAHVKNLRRKIEPEVGHPRYVLTVHG
                     VGYRFADA"
     gene            complement(1506..1916)
                     /locus_tag="VIX62_07760"
     CDS             complement(1506..1916)
                     /locus_tag="VIX62_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860163.1"
                     /translation="MRRGIGIVAVVVVILAGIGIGVGAYNAGERNGIAQGIEQVQVAQ
                     DNGQEVQVVHVVGDEHRGFFPGFFLFPLFLIGVFFLIGGIMRGAGRWGRGGPGGYGPG
                     PWNEEGRRRFEERAREWHQREHGDTPPAEASPAG"
     gene            2051..2479
                     /locus_tag="VIX62_07765"
     CDS             2051..2479
                     /locus_tag="VIX62_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5860164.1"
                     /translation="MPASEPRRMEREAMAMVGEMTAVLQGMFGALDKSDPEMALASIG
                     ADAQGIDEISRRWMRGPGEVTRYVRELFGIAEDVHSEMHDVHEVDWGETGVVTFWLEQ
                     DYTLDGDRGHVSAPTTAVLRRHGGAWKLALFHSLPLPPEP"
     gene            complement(2491..2997)
                     /locus_tag="VIX62_07770"
     CDS             complement(2491..2997)
                     /locus_tag="VIX62_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860165.1"
                     /translation="MKLRDVGPDDLALWERLRCDPAMNTELGGPQPREEIPGKLAEDV
                     AAVNTDTSWIVVAESDDGEAMGSVCIWRHDDDAPFSEIGWGVLPEFQGRGVGKRSVAA
                     LLERAGADGRWGTIHAFPAITNAPSNGICRSLGFTLIGQEAFEFRGTTLHCNHWQIDP
                     AEDLDPLP"
BASE COUNT          467 a         1116 c         1021 g          470 t
ORIGIN      
        1 gcctcgatcg tgccgtccgt tccggcatcg gtcgcgatcg tcagcgtgat gccggcctcg
       61 tcagccatcg ttcggaacgc cgtggcgacg tcacgggcga gagcccgagg gtcgacggtc
      121 tcgcggtgca gctcgagcac gccggcctcg gccatggaga gcgtgcgcag atcctccagg
      181 aggcgatcca tcacgacggt ctcctccacc agcggacgca gccgttcggc atcggcaggg
      241 tagaggccgt cgagcatgcc ctcgacggtg ccgcggatga cctgcagcgg tgtccggagc
      301 tcgtgcgcga cgtcggccag caactcacga cgccgcgcct catcggtctc gaggcgttcg
      361 gtcatctggt tgaagctgga cgtcagccgt cccagctgcc gcgaacccgt gacctcgacc
      421 cgggtggtgt agtcgccgcc ggccacgcgg tcggcggcgt ccatcaacgt cccgatcgcg
      481 cgcgtgttcc gccagagcca gcgcgcgctc gcgatcaagg ccgcgatgac ggcgagggcg
      541 agcggcaccg tcacgacggg gtgcggcgcg ttgccgccga acgcggacgc caccaccgtc
      601 gcgagcgccg ccaccagcag gaccaccagg accagcgcgg cgatcgctcc ccggcggaac
      661 cgtcgtcctg cgtcgaaccc cggccgtcct ctgcccgccc acggcggccg gccgtcccag
      721 tccggaccgc gccacgggcc tccacggcgg tcgtcgtgct caggcatcgg cgaaccggta
      781 gccgacgccg tgcaccgtga gcacgtagcg agggtgtccc acctcaggct cgatcttgcg
      841 acgcaggttc ttcacgtgcg cgtcgatcgc gcgctcgtac gtgtcgaccg tgacgccgtg
      901 cagggcatcc aacagttggc cgcgcgtgaa caccctgccc ggttcccgga cgaacgtcgc
      961 caggagctgg aactccgtgg gggtgaggtc gaccgcgcgt ccgtccacgc gagcgcgcat
     1021 cttcggcagg tcgacctcca cgccgccggc acgcatcact tcggctccgg ccgttgttcc
     1081 gttcgtgcgc cgcaggaccg cacgcaccct ggccaccagc tccttgggac tgaagggctt
     1141 gacgaggtag tcgtcggcgc cgagctctag cccgacgatg cggtccgatt cctcgccgcg
     1201 cgcggtgagc atcacgatcg gtgtggccga cgtgcggcga agttcacgcg ccacgtcgag
     1261 gccgtccatc cccggcaacc cgaggtccag caccacgagg tcgggacgat cgccccgcgc
     1321 actggcgagc gccgctgagc cgtcgcccac cacggtcaca tcgaacccgg cgacctcgag
     1381 gtagtcgcgc aacgtccgcg cgatctgcat ctcgtcttcg accaccagga tccgctgcat
     1441 ggggcgagcc tccctcgcga gacgggcgga ggcaaccgtg cctccgcccg cgcctcgcga
     1501 cctcgtcagc ctgcaggcga tgcctcggcc ggcggtgtgt cgccgtgctc acgctgatgc
     1561 cactcgcgag ctcgttcctc gaaccggcgc cggccctcct cgttccaggg tcccggcccg
     1621 tagcctccgg gcccgcctcg accccaacga cccgctcccc gcatgatccc gccgatcagg
     1681 aagaagaccc cgatcaggaa cagggggaac aggaagaacc ccgggaagaa cccgcgatgc
     1741 tcatcgccca ccacgtgcac gacctggacc tcctggccgt tgtcctgggc gacctgcacc
     1801 tgctcgatgc cctgggcgat gccgttgcgc tcgcccgcgt tgtacgcgcc gaccccgatg
     1861 ccgatccccg cgaggatcac caccaccact gccacgatcc cgataccacg acgcatgtcg
     1921 tacctcctcg tctcgcgtct gcctccacgt tggcgcccag atgtgaaccg ttcgtgaagg
     1981 acgtggggag gttcatcggt gcctccctgg ctctcgaggc agcaccaggc gtatggtcga
     2041 ggttcaggtc atgcccgcat cggaaccacg ccgtatggaa cgggaggcga tggcgatggt
     2101 cggtgagatg accgcggtac tgcagggcat gttcggtgct ctcgacaaat ccgatcccga
     2161 gatggcgctt gcctcgatcg gtgccgatgc gcagggcatc gacgagatct cacgtcgatg
     2221 gatgcgaggg cctggtgagg tcacccggta cgtccgtgaa ctcttcggca tcgccgagga
     2281 cgtccactcc gagatgcacg acgtgcatga agtcgactgg ggcgagaccg gcgtcgtcac
     2341 cttctggctg gaacaggact acacgctgga cggcgatcga ggacacgtgt ccgcgcccac
     2401 caccgcggtg ctgcgacgcc acggcggggc ctggaagctg gcgttgttcc actccctgcc
     2461 cctgccgccg gagccgtaga caccggccga tcacggaagc gggtcgagat cctcggccgg
     2521 gtcgatctgc cagtggttgc agtgcaaggt cgttccgcgg aactcgaacg cctcctgccc
     2581 gatcagcgtg aagccgagcg agcggcagat gccgttggac ggcgcgttgg tgatcgcggg
     2641 gaaggcgtgg atcgtgcccc agcgaccgtc cgcaccggcg cgttccagca gcgccgccac
     2701 cgagcgcttg cccacgcccc ggccttggaa ctcgggcagc acgccccagc cgatctcgct
     2761 gaacggcgcg tcgtcgtcgt gacgccagat gcacaccgat cccatggcct cgccgtcgtc
     2821 ggattcggcc acgacgatcc acgaggtgtc ggtgttcacc gcggcgacat cctccgcgag
     2881 cttgcccggg atctcctccc gtggttgcgg accgccgagc tcggtgttca tggccgggtc
     2941 gcagcgcagg cgctcccaca gcgcgaggtc gtcgggtccg acgtcccgga gcttcatccc
     3001 agcatcttgc cacgggtcat cgcggcgtag ggtggtggag cgaggccgcg aggaggttcg
     3061 acatgaacga cgcc
//
LOCUS       DATDBZ010000320         3440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2963949, whole genome shotgun sequence.
ACCESSION   DATDBZ010000320 DATDBZ010000000
VERSION     DATDBZ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2963949"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VIX62_07775"
     CDS             complement(<1..426)
                     /locus_tag="VIX62_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5860166.1"
                     /translation="MVEGYDVIVVGGGTAGSVVAGRLVEAGRHVLVLEAGPDYGSFGT
                     LGWPSDLLDAATIPTSHDWGYRSGDELLGRALPYERARVIGGCSTHNGCTVSWGHRLD
                     YDGWAARGLDGWRADDLLPLFGEASLRMRVRRFRDDEVAP"
     gene            492..1181
                     /locus_tag="VIX62_07780"
     CDS             492..1181
                     /locus_tag="VIX62_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860167.1"
                     /translation="MPEGANVEIAKHLNEHGSHSEGGHSDRFSRILEIFEAILLACVA
                     IATAWSGYQSAKWDGRSAEQYAEASKLRVEQDLAATQAGQQLLFNTNALNAWVTAITT
                     GDDQAAAVFAKRFTPNYAVAFDAWLETDPLNDADAPAGPIFMPEYHSPLDDKAADLDE
                     QATRALEQGGSSRDTGEEYVRVTVFLAMVLFLIAMSQRFKIRNVRLGLIGLAVVFTGF
                     ALTLILTFPHL"
     gene            complement(1162..1932)
                     /locus_tag="VIX62_07785"
     CDS             complement(1162..1932)
                     /locus_tag="VIX62_07785"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEY5860168.1"
                     /translation="MTTDDPMVDPASPHHGRPHPIRHLLRSLLSPHSYGSVLILIAIT
                     YGFSTVATERWQRSVVLLIQVITVWIALRVSHARRPVLRFADGVLVIAILVAITTLFG
                     DDGKEVSAAVFLVAAMLYFIAPLSILRHIVQQPDVDQETVLGAIDAYLLVGMFFAYAY
                     QAIGAIQAGPFFEGGIDGSVAQSLFFSFTTLTTTGYGNLVPAANPGQTFAVMEMLIGQ
                     LFLVTAVAKVINLWRPARWRAGQADGEAGEPPVTGGGR"
     gene            complement(1934..3238)
                     /locus_tag="VIX62_07790"
     CDS             complement(1934..3238)
                     /locus_tag="VIX62_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY5860169.1"
                     /translation="MELVRDRRYVVVLVMGALVGVPVALVAYFFLKWVADVQQYVFTT
                     LPADLGFNGEPSWWPIPPLIICGVLVGLTIKHLPGTAGHEPAQGFKTTGPVPPIDLPG
                     IIIASFATLGLGVVLGPEAPLIAIGSGMGALAIRLLKKDAPKQAVMVISAAASFAAIS
                     TLLGSPLAGAFLLMEAAGLGGPMLGVVLVPGLLAAGVGSLIFIGLDQLTGFGTFSLSV
                     PDIPTFTTPTVGEFAWAIGIGLAAAVLGTAIKRLALWLQPIVAKRRVMVTPAMGLVIG
                     ICALVFAETTGRSPNEVLFSGQSALPALIENAAGWTVGALVVLIICKGLAYGAALSAF
                     RGGPTFPGMFIGAAAGIALSHLPGLPMIAGVAIGIGAMTTVMLGLPLTSVLLTALFLS
                     ADAVPLMPLVIVGVVVAYVASARLAPVPAATDGQPLPAEAAT"
BASE COUNT          620 a         1163 c         1168 g          489 t
ORIGIN      
        1 cggggcgacc tcgtcgtcac ggaaccgtcg aacccgcatc cgcagggacg cttcgccgaa
       61 cagcggcagg aggtcgtcgg cccgccaacc atcgagccct cgcgccgccc agccgtcgta
      121 gtcgaggcga tgcccccagg aaacggtgca gccgttgtgg gtggaacagc ccccgatcac
      181 ccgggcgcgt tcgtacggca gggcgcggcc gaggagttcg tctcccgagc ggtaccccca
      241 atcgtggctc gtggggatcg tggcggcgtc gagcaggtcg ctgggccacc ccagcgtgcc
      301 gaacgagccg tagtcggggc cggcttcgag caccagcacg tgccgccccg cttccaccag
      361 ccggcccgcg acgaccgacc cggcggtgcc gccgcccacc acgatcacgt cgtagccctc
      421 caccatggcg gtggagtcta ggggcgctgc gaggacgtag catccaggca ccatgcgcgg
      481 gaggaacgaa cgtgcctgaa ggtgcaaacg tcgagatcgc caagcatctc aacgaacatg
      541 gcagtcacag cgagggtggc cacagcgacc ggttcagccg catcctggag atcttcgagg
      601 ccatcctttt ggcgtgcgtc gcgatcgcga ccgcctggag cggctaccag tcggcgaaat
      661 gggacggccg atccgccgaa caatacgctg aggcgtccaa attgcgggtg gagcaggacc
      721 tcgccgccac gcaggccggc cagcaactgc tgttcaatac gaacgccctc aacgcgtggg
      781 tgaccgccat caccaccggt gacgatcagg ccgccgccgt gttcgcgaag cggttcaccc
      841 cgaactacgc agtcgcgttc gatgcctggc tcgagaccga cccgctcaac gacgccgacg
      901 ctccggcggg gccgatcttc atgccggagt accacagccc gctcgatgac aaggccgcag
      961 acttggacga gcaggcgacg cgcgcgttgg agcagggcgg gtcctcgagg gacaccggcg
     1021 aggagtacgt ccgcgtcacg gtcttcctcg cgatggtgct gttcctgatt gcgatgtcgc
     1081 agcggttcaa gatccggaac gtgcggctcg gactgatcgg tctcgccgtc gtgttcaccg
     1141 gattcgccct gacgttgatc ctcaccttcc cccacctgta accggcggct cgccggcctc
     1201 gccatcggcc tgccccgccc gccagcgggc agggcgccag aggttgatga ccttcgccac
     1261 cgccgtcacc aggaatagct gcccgatcag catctccatc accgcgaagg tctggcctgg
     1321 gttggcggcc ggcaccaggt tgccgtagcc cgtcgtggtg agcgtcgtga agctgaagaa
     1381 cagcgattgc gccacggagc cgtcgatccc gccctcgaag aacggcccgg cctggatcgc
     1441 gccgatggcc tgatacgcgt aggcgaagaa catgcccacc aacaggtagg cgtcgatggc
     1501 gcccagcact gtctcttgat cgacatcggg ctgctggacg atgtgccgca ggatcgacag
     1561 cggagcgatg aaatacagca tcgccgccac gaggaagact gcggccgaca cctccttgcc
     1621 gtcgtcgccg aacagggttg tgatggcgac caggatcgcg atcacgagaa cgccatcggc
     1681 gaaccggagc accggtctgc gagcgtggga gacccggagg gcgatccata ccgtgatgac
     1741 ctggatcaac agcaccaccg accgttgcca ccgttcggtg gcgacggtgg agaacccgta
     1801 ggtgatcgcg atcaggatga gcaccgaccc gtagctgtgg ggcgagagca gcgaacgcaa
     1861 caggtgtcgg atcgggtgcg gtcgcccgtg atgcgggctc gccgggtcaa ccatcggatc
     1921 gtcggtcgtc atgtcatgtc gccgcttcag ctgggagcgg ctgcccatcg gttgcggccg
     1981 gaacgggggc gagtcgggcg ctggcgacgt aggccaccac gacgccgacg atcaccaacg
     2041 gcatcaacgg gacggcgtcg gcggacagga acagggccgt cagcaggacc gacgtgagcg
     2101 gaagccccag catgacggtc gtcatggcac cgatcccgat ggcgacgccc gcgatcatgg
     2161 gcaagccagg caggtgggac agcgcgattc ctgcagccgc accgatgaac atgccgggga
     2221 acgtggggcc tccccggaat gcgctcagcg ccgctccata cgccagcccc ttacagatga
     2281 tcagcacgac gagggcgccg accgtccacc cggcggcgtt ctcgatgaga gccgggaggg
     2341 cggactgacc ggagaagagg acttcgttcg gactccgtcc cgtggtctcg gcgaagacga
     2401 gggcgcagat gccgatcacg agccccatcg cgggcgtgac catcacgcgt cgtttcgcga
     2461 cgatgggttg cagccagagt gccaaccgtt tgatcgcggt gccgaggacc gcggcggcga
     2521 gaccgatccc gatcgcccag gcgaattcac cgacggtcgg ggtcgtgaat gtggggatgt
     2581 cggggaccga cagcgagaac gtcccgaacc ccgtcagttg atcgaggccg atgaagatga
     2641 gcgagcccac tcccgccgcg agcagacccg ggaccagcac gacgcccagc atcggcccac
     2701 ccagcccggc cgcttccatg agcaggaacg caccggccaa cggcgagccg aggagggtgc
     2761 tgatcgcggc gaagctcgcc gcggccgaga tcaccatgac cgcctgcttg ggggcatcct
     2821 tcttcaggag gcggatggcg agggcaccca tcccactgcc gatcgcgatc agcggggcct
     2881 cgggccccag gacgaccccg aggcccagcg tggcgaacga cgcgatgatg atgcccggca
     2941 gatcgatggg cggcaccggt cctgtggtct tgaatccttg cgcgggttcg tggccggcgg
     3001 tccccggcag gtgcttgatc gtgagcccca cgagcacccc gcagatgatc agcgggggga
     3061 tcggccacca ggacggctct ccgttgaacc cgaggtcggc gggcaaggtc gtgaacacgt
     3121 actgctggac atcggccacc cacttgagga agaagtacgc gaccagtgcg acgggcaccc
     3181 cgaccagggc gcccatgacg agcaccacca cgtagcgccg gtcccgaacg agttccaacg
     3241 gggccatggg aggcgtcgcc tgctcaggtg tcggatccga gaccttcgga ggcccccctc
     3301 cggatgccat cgcctgtccc ccctcggccg ttcacgtgtt cacgggtcga tctcggtgtg
     3361 tgccagcggg catcatccca tgcgctccgc cctcgcggtg gctacgaacc cgcggatgcc
     3421 ttgggctgct ggacgcagcg
//
LOCUS       DATDBZ010000321         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_320798, whole genome shotgun sequence.
ACCESSION   DATDBZ010000321 DATDBZ010000000
VERSION     DATDBZ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320798"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VIX62_07795"
     CDS             complement(<1..443)
                     /locus_tag="VIX62_07795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5860170.1"
                     /translation="MRRAVRTVLEQEHPGTIEAVVVFDACEPTELAVDDLLGPTRTVT
                     VLTNDRTRGPAGTRNRGIEASRGSWVAFLDDDDAWLAGKLTRQLAELKRTGAGVATCG
                     VRYVADGRYRDQIPTVQADMQRAIVAGGTFVPLQTMVVRRDHLDRA"
     gene            complement(505..1479)
                     /locus_tag="VIX62_07800"
     CDS             complement(505..1479)
                     /locus_tag="VIX62_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY5860171.1"
                     /translation="MPQGSAPDGALTVLYIGGYGRSGSTVLDRVLGALPDAVSVGEVR
                     SIWGAVLREDRLCACGQRFSACPFWTEVGDRAFGGWDSPAVERARAAHGLVDRHRHLP
                     QLLSGKGSATFAREEAVFTQALGDLYRAIAETGGARVVVDASKYPVYASILRKVPAVD
                     LRVVHLIRDARGAAYSWSKEGVVKPDASGDGTFMPTYRASRASAEWLFYNAGFDALKA
                     MRIPTMRLYYESFVRDPRGSVARVARFAGLTADEESLSYIHGPTVRLGGPEHALGGNP
                     ARFGDRDVLTLRADQAWRERMAPGQRRTVTALTSPFLVRYGYVGRHGR"
     gene            complement(1485..>2045)
                     /locus_tag="VIX62_07805"
     CDS             complement(1485..>2045)
                     /locus_tag="VIX62_07805"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEY5860172.1"
                     /translation="VHAYFGVPNDRGLVQVLDGGDEPPLVMTGVPNLRLLPSGPPVEM
                     SAQLFEGGAFPELVDRLREAADLVIIDAPPLAISDPLVMAEHADGILIVVDMKQADKS
                     GLRRVRELLERAGLTPLGLILNKFEGEWSRKYGAYGAYYSPYRGAGGDVAPGKTSRRD
                     AKAAAAGQKQLGREAAQRQRDQGRRA"
BASE COUNT          319 a          750 c          690 g          286 t
ORIGIN      
        1 gctcgatcca gatggtcgcg acgcacgacc atggtctgca ggggaacgaa cgtgccacca
       61 gccacgatcg cgcgctgcat gtccgcctgc accgtgggga tctgatcgcg gtagcgaccg
      121 tccgcgacgt atcgcacccc acaggtagcc accccggcgc cggttcgctt cagctcggcg
      181 agctgacgcg tgagcttgcc ggccagccac gcgtcgtcat catcgaggaa ggcaacccac
      241 gaccctcggc tcgcctcgat cccgcggttc cgcgtgccgg cggggccacg ggtccggtcg
      301 ttcgtcagga cggtgacggt ccgagtgggg ccgaggagat catcgaccgc gagctcggta
      361 ggctcgcacg cgtcgaagac cacgaccgcc tcgatcgtcc ccgggtgttc ctgctcgagc
      421 acggtgcgga cggcgcgtcg caacggcgct ccaccccggt gagaggggat cacgaccgag
      481 acgtccggcg acggggatcc gccgtcagcg gccatgacgt cccacgtacc cgtatcgcac
      541 caggaacggc gacgtcagcg ccgtgaccgt ccggcgctgg cccggggcca tccgctcccg
      601 ccacgcctgg tccgcgcgca gggtgagcac gtcccggtct ccgaaccgcg ccgggttccc
      661 gccgagcgcg tgttcggggc cacccagtcg aaccgtcgga ccgtggatgt aggacagact
      721 ctcctcgtcg gccgtcaggc cggcgaaccg cgcaaccctc gcgaccgagc cgcgaggatc
      781 gcggacgaac gactcgtagt acaggcgcat cgtcgggatc cgcatcgcct tcaacgcatc
      841 gaaccccgcg ttgtagaaca accactcggc gctggccctc gacgctcgat acgtgggcat
      901 gaacgtgccg tcaccgctgg cgtcgggctt gacaacccct tccttcgacc acgagtaggc
      961 cgcgcccctg gcgtcccgga tcagatgcac gacgcggagg tcgacggccg gcaccttgcg
     1021 caggatcgac gcgtagacgg ggtacttgct ggcatccacc accacacggg cgccgccggt
     1081 ttccgcgatc gcccgataga gatccccgag cgcctgcgtg aacacggcct cctcccgggc
     1141 gaaggtcgcc gatcccttgc ccgacagcaa ctggggtagg tgccggtgcc ggtcgacgag
     1201 accgtgcgcc gcgcgcgcgc gctcgaccgc gggcgagtcc cagccaccga acgcccggtc
     1261 tcccacctcg gtccagaagg gacacgccga gaaccgttga ccgcacgcgc agagccggtc
     1321 ctcacggagc accgctcccc agatcgagcg gacctctccc acggagacgg cgtccggaag
     1381 cgcgccgagg acgcggtcga ggacggtgct cccgcttcgc ccgtagcccc cgatgtagag
     1441 caccgtgagc gccccgtcgg gcgccgagcc ctgcggcatc gtggtcaggc ccggcggccc
     1501 tggtcacgct ggcgctgggc cgcttcacga ccgagctgct tctgcccggc tgcggcagcc
     1561 ttggcatccc gccgtgaggt cttcccggga gccacatcgc caccggcgcc gcggtacggc
     1621 gagtagtacg ccccgtaggc gccgtacttg cgggaccatt cgccttcgaa cttgttgagg
     1681 atgagcccaa gcggcgtcag gccggcccgt tcgagcaatt cacggacacg gcgcaggccg
     1741 ctcttgtcgg cctgcttcat gtcgaccacg atcaggatgc cgtcggcgtg ctcggccatc
     1801 accagcgggt cggagatggc gagcgggggc gcgtcgatga tcaccaggtc ggcggcttca
     1861 cgaaggcggt cgaccagctc cgggaacgca ccgccttcga acagctgggc ggacatctcg
     1921 acgggagggc cgctcggcag cagccggagg ttgggcaccc cggtcatgac caggggcggt
     1981 tcgtctccgc cgtcgagcac ctgcaccaag ccccggtcgt tcgggacccc gaagtaggcg
     2041 tgcac
//
LOCUS       DATDBZ010000322         7098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1953608, whole genome shotgun sequence.
ACCESSION   DATDBZ010000322 DATDBZ010000000
VERSION     DATDBZ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953608"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VIX62_07810"
     CDS             complement(<1..357)
                     /locus_tag="VIX62_07810"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY5860173.1"
                     /translation="MTHPLEQLAPYVDGSLTGPERLEVERHLASCAQCRSEVAVASEA
                     RAALRSMPEVEVPTDLGVPALEVMGTGEAHHPGPPRWVRAMPFVAAAAVVALLAITLP
                     RLGGGTAADSGAADQAL"
     gene            complement(354..974)
                     /locus_tag="VIX62_07815"
     CDS             complement(354..974)
                     /locus_tag="VIX62_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5860174.1"
                     /translation="MKEPAPDDDLVRLAAGGDRDAFGTLVERHGSNIYRLCLRIMGNP
                     EDAHDAAQDTFLVAMRKLDQFRGEAAFGTWLHRIGVNACYDALRRKKRQPMLRSVDPD
                     DGPQPDLGPAEDDRADEIVGTMGVAQALAEIAEDYRVAVVLADVQDMAYDDIAKILDV
                     PVGTVKSRVHRGRIALAKVLGLEPGARGPRRDGEPIGGAGTSEGQS"
     gene            complement(985..1398)
                     /locus_tag="VIX62_07820"
     CDS             complement(985..1398)
                     /locus_tag="VIX62_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860175.1"
                     /translation="MEMVNRAHRDQRGLVGKFMIVWLLLLAVIVLVGYDAVSIAITTF
                     KVSDVAAQAASDAVVEYRHERDLTAACAVAQATIAADQPDLKLGKNFCTGDPEVVAIT
                     ITIRTTAHTFALGRLDFTKHYTNITQTETAGESAV"
     gene            complement(1398..3098)
                     /gene="murJ"
                     /locus_tag="VIX62_07825"
     CDS             complement(1398..3098)
                     /gene="murJ"
                     /locus_tag="VIX62_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY5860176.1"
                     /translation="MVEEVHPTPEELMPPPEELPELVELPEEDSRAAYARNTAVMTVG
                     TTLSRITGFLRIAAQTAALGVLVGSLGDTYTRANTTPNIVYELILGGILTSIFVPVFV
                     EWRNKYGREAAWDVGRRVLTLTLVVLSVVTVLGMIFAPQIMRLYLVFSDAADRESQIE
                     LGAYLLRWFMPQIVFYGIGAVASGLLNSERRFAAPMFAPVLNNLVAIATFVGYAMLRS
                     SATPSVDQITTMEKLVLGAGTTLGVVAMTVALWPSLRSIGFRWKLTGGWNHPAVRRLG
                     KLSLWVIVYVFANQLAYLVINILAGGIGEGRFQIYATAFIVFSLPHAIFGVSIFTALL
                     PGMSQQWTDGRPAGVVALLSRGIRDTVVVIVPAAFAFVVIAVPIVRLLLQYGEAGPQD
                     TALIARTLQGFALGLPFFSIFQLLTRTFYAMQDSRTPALVNVGAAFVTIVIDVVFTVV
                     LDWDVPGLAMGHAISYAFATAVALFLLRGRLRSLDGARIWRTIGKVVPAAALTAGAAW
                     LVATGAGHVVDTATVVGRIVQVGSAIAVGLGVYLAATLMLHIEEVDEVKGAVLRRFRG
                     "
     gene            complement(3062..5125)
                     /locus_tag="VIX62_07830"
     CDS             complement(3062..5125)
                     /locus_tag="VIX62_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860177.1"
                     /translation="MSIHRALRASFALSLIVAACLLPAVGVQAQEPLVSLTLFSQTPV
                     TTNSEPELELVVRAVNAGDAAIGDLSLGFSIGPKIISRFVYEQALIDGPGLAPISTTS
                     LPQEGTLEPGQERLFTVKIDMTSIPEIADPTQDSGVYPSQVSIRSADAPIATLNTPLI
                     HLVRAPEQPLQLAWWTEVSGPIAFGPDGRLSDSSLEAAVAPEGALAMQAAALLRMDED
                     PDLRSTIDLVVEPLLLDQLDRMADGYERSDGTTVPADEAPATNAATVLKTLRKVVERN
                     AVQLSAMPFSAPLLPAMVSGGLADDLQDQRVVGAETTESLLGHAPVTSVARPPTGAID
                     DPALDDLAAQGIGAVLANADAVDRTAQLNDFAPLPAATITTPSGASMDLVLPDPSVQG
                     LLADPELIADPVRAAQAVLGEIATIWREQPVPGPQPDDSPTVRGVAVSLPTSLPPTIW
                     GPITRRLAGATFLAPLHAQDFAATVNPVEPALALAAPSTARFRPDYIEKLHDERKDVE
                     AYRSMLVEDNPAPERLDRDLMIAEAGVYVFDELGGRRWIDQVNAFTDDVFQRVLPVDT
                     QAFTLTSDEGSLPLRMGDPGETPLQVQIQLRSSRFDFPDGNEQTVTLTEPDQVVVFTV
                     QAKAAGSQTIKVRTRAPSGRSLAESNLTVRTTAVNSIAVIITGAAALLLVALWSRRYI
                     RRPKS"
     gene            complement(5156..5935)
                     /locus_tag="VIX62_07835"
     CDS             complement(5156..5935)
                     /locus_tag="VIX62_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5860178.1"
                     /translation="MTVFEAILGVLIGLVAGVLSGLFGVGGGIVMTPGIQVLLQTSPI
                     VALATPLPVILPTAFTGAMTYRKAGEIDMRAVAWMVGPGIVGSIAGAALTDVVNTHLL
                     LVITAVLLAWQAADILRGARARVDTGEGPRFETTPSMYLGIGLVAGFVSGLLGIGGGL
                     IMVPLLVGWLGMPLKRALGTSLLTIVALVIPGTIVHAALGHIDWAIFAVVTLGAVPGA
                     RLGAHIALGTKERTLRLLVGSFLLVVALAYGTTEAIELLGS"
     gene            complement(5932..6588)
                     /locus_tag="VIX62_07840"
     CDS             complement(5932..6588)
                     /locus_tag="VIX62_07840"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5860179.1"
                     /translation="MSEGSTPDGGDAPKRKRRRRRRRRPGSQAATQTTQAQASDPNAK
                     EPAEQQPRAKGKQPSKGKKKRTKKAGRPHNQGKGPVQREISAGGVVYRRDGEEIEVVL
                     ASRRTRRGELAWGLAKGGIEEGESHEQAAVREVKEETGYDAEIEASLGDTRYFYVWED
                     TRIRKTVHFFLMRCTGGDPDDRDDEMEDVRWFPLDRALKRAAYRGEREVLGRAADLLR
                     "
     gene            6708..>7098
                     /locus_tag="VIX62_07845"
     CDS             6708..>7098
                     /locus_tag="VIX62_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HEY5860180.1"
                     /translation="MEIDELLRYTVERGASDLHLKVGNVPFIRVDGTLTPTDFDVLTS
                     TDTESFAMLLMSEHKKREFSETSEADLGTTLTGVGRFRVNVFRQRGVVGLAIRRVRSE
                     VPTFEELRLPPVMEALGDSPRGLVLITG"
BASE COUNT         1120 a         2380 c         2516 g         1082 t
ORIGIN      
        1 aagcgcttgg tcggcggcgc cgctgtccgc cgcggtcccg ccgcccaggc gaggcagggt
       61 gatcgccagg agcgccacga cggcggcggc ggccacgaag ggcatggccc gaacccagcg
      121 cgggggaccc ggatggtgcg cttcacctgt ccccatcact tcgagggcgg ggacgccgag
      181 atcggtcggg acctcgacct cgggcatcga tcggagggcg gctcgggcct cgcttgcgac
      241 ggccacctcc gagcgacact gggcgcacga ggccaggtgg cgttcgacct ccagacgctc
      301 gggccccgtg agggagccgt cgacgtaggg ggcgagttgc tcgaggggat gcgtcatgac
      361 tgcccctcgg acgtcccggc cccgccgatg ggttccccgt cacggcgtgg gccgcgcgcg
      421 ccgggctcga ggcccagcac cttggcgagc gcgatgcgtc ctcggtggac ccgcgacttc
      481 acggtgccca ccggcacgtc gaggatcttc gcgatgtcgt cgtaggccat gtcctgcacg
      541 tcggcgagca ccacggcgac gcggtagtcc tcggcgatct cggccaatgc ctgggctacc
      601 cccatcgtgc ccacgatctc gtccgcgcgg tcgtcctcgg ccgggccgag gtccggctgc
      661 ggtccgtcgt cgggatcgac cgaccgcaac atgggctgtc gcttcttgcg ccgcaacgcg
      721 tcgtagcagg cgttcacgcc gatgcggtgg agccaggtgc cgaaggccgc ctcgccgcgg
      781 aactggtcga gcttgcgcat cgccaccagg aaggtgtcct gcgccgcgtc atgagcgtcc
      841 tcggggttgc ccatgatccg caggcacagc cggtagatgt tcgacccgtg ccgctcgacc
      901 agcgtgccga acgcgtctcg gtccccgccg gcggcgagcc ggacgaggtc gtcgtcgggg
      961 gccggttcct tcacgtgtcg gacgctacac ggcggattcg ccggcggtct cggtctgggt
     1021 gatgttcgtg tagtgcttgg tgaagtccag ccggcccagc gcgaacgtgt gcgcggtggt
     1081 tcggatcgtg atcgtgatcg cgacgacctc gggatcaccc gtacagaagt tcttgccgag
     1141 cttcagatcg ggctggtcgg cggcgatcgt ggcctgggcg acagcgcagg cagcggtcag
     1201 gtcgcgctcg tgccggtact ccacgaccgc gtcgctcgcg gcctgtgcgg ccacgtccga
     1261 gaccttgaac gtcgtgatcg cgatggacac cgcgtcgtat cccaccagca cgatgacggc
     1321 cagcaggagc agccagacga tcatgaattt gccgaccagc ccacgctggt cccgatgtgc
     1381 tcggttcacc atctccatca cccgcggaac ctcctcagca ccgcgccttt gacttcatcg
     1441 acctcttcga tgtgcaacat gagcgtcgcg gccaggtaca cgcccagtcc cacggcgatc
     1501 gccgacccca cctggacgat gcggcccacg accgtggcgg tgtcgaccac gtgcccggcg
     1561 ccggtggcga cgagccaggc tgccccggcg gtcaacgcgg ccgccggcac caccttgccg
     1621 atggtcctcc agatccgtgc cccatccaag gacctgaggc gtcctcgcag gaggaacagc
     1681 gcgacggcgg tcgcgaacgc gtacgagatc gcgtggccca tggcgagccc gggaacgtcc
     1741 cagtccagga ccacggtgaa gacgacgtcg atgacgatcg tgacgaacgc tgccccgacg
     1801 ttcaccaagg cgggcgtgcg gctgtcctgc atcgcgtaga aggtgcgggt gagcagctgg
     1861 aagatcgaga agaacggcaa ccccagagcg aacccctgga gggttcgggc gatcaatgcc
     1921 gtgtcctgcg ggcccgcttc gccgtattgg agcagcagcc ggacgatcgg cacggcgatc
     1981 acgacgaagg cgaacgcggc cggcacgatc acgacgacgg tgtcccggat gccacgggac
     2041 aacagggcga ccacgccggc gggcctgccg tcggtccatt gctgagacat gccgggtaac
     2101 aaggcggtga agatcgagac gccgaagatc gcgtgcggca acgagaagac gatgaacgcg
     2161 gtggcgtaga tctggaagcg gccctccccg atgccgccgg ccaggatgtt gatgaccagg
     2221 taggcgagct ggttcgcgaa cacgtagacg atgacccaga gcgagagctt gcccaggcgg
     2281 cgcaccgccg ggtggttcca gccgccggtg agcttccaac ggaacccgat cgaacgcagc
     2341 gagggccaca aggccacggt catcgccacc acgccgagcg tggtgccggc gcccagcacc
     2401 agcttctcca tcgtcgtgat ctggtcgacc gatggggtcg ccgagctgcg gagcatcgcg
     2461 tacccgacga acgtggcgat cgcgacgagg ttgttgagga cgggggcgaa catcggcgcg
     2521 gcgaaccggc gctcggagtt cagcagaccg ctcgccacgg cgccgatccc gtagaacacg
     2581 atctgcggca tgaaccagcg caacaggtag gcgcccagtt cgatctggga ctcgcggtcg
     2641 gcggcatcgg agaagaccag gtacagccgc atgatctgag gggcgaagat catgcccagg
     2701 acggtcacga ccgacaggac caccagggtc aacgtgagga cgcggcggcc cacatcccag
     2761 gcggcttcgc gcccgtactt gttccgccac tccacgaaca ccggcacgaa gatcgacgtg
     2821 aggatcccgc ccaggatcag ctcgtagacg atgttcgggg tggtgttggc tcgggtgtag
     2881 gtgtcgccga gcgaccccac cagcaccccg agggcggccg tctgcgccgc gatccgcagg
     2941 aacccggtga tccgcgacag cgtggtgccc acggtcatca ccgcggtgtt gcgcgcgtag
     3001 gcggcgcgcg agtcctcctc gggcagctcg acgagttccg ggagctcctc gggcggcggc
     3061 atcagctctt cgggcgtcgg atgtacctcc tcgaccacag agcgaccaac agcagggccg
     3121 cggcgccggt gatgatgacc gcgatcgagt tcaccgcggt ggtgcggacg gtcaggttcg
     3181 attcagcgag cgatcggccg gagggggcgc gggttcgcac cttgatggtc tgcgatcccg
     3241 cggccttggc ctgcacggtg aacaccacca cctggtccgg ctcggtgagc gtcacggtct
     3301 gctcgttgcc gtcggggaaa tcgaaccggg acgatcgcag ctggatctgc acctgcagcg
     3361 gtgtctcgcc cggatcgccc atccgcagcg gcaggctgcc ctcgtcggag gtgagggtga
     3421 aggcctgggt gtcgaccggc agcacccgtt ggaacacgtc gtcggtgaag gcgttcacct
     3481 ggtcgatcca ccgccggccg ccgagttcgt cgaagacgta gacgccagcc tcggcgatca
     3541 tgaggtcgcg gtcgagccgc tcgggggcgg ggttgtcctc gaccagcatc gagcggtagg
     3601 cctcgacgtc cttgcgctcg tcgtgcagct tctcgatgta gtccggacgg aaccgtgcgg
     3661 tcgatggagc ggccaaggcc agcgcaggtt ccacgggatt caccgtggcc gcgaagtcct
     3721 gtgcgtgcaa gggcgcgagg aaggtggctc ccgccaggcg gcgggtgatc ggtccccaga
     3781 tcgtgggcgg gagcgacgtg ggcagcgaca cagccacgcc gcgaacggtc ggggaatcgt
     3841 cgggttgcgg cccgggcacc ggctgctcgc gccagatggt ggcgatctcg cccagcaccg
     3901 cctgggcggc acggacgggg tcggcgatca gctccgggtc ggccaacagg ccttggacgg
     3961 agggatcggg gagcacgagg tccatcgagg cgccggaggg cgtggtgatg gtggcggccg
     4021 gcaacggcgc gaagtcgttc agctgcgccg tgcggtcaac cgcgtcggcg ttggccagca
     4081 ccgcgccgat cccttgggcg gcgaggtcgt cgagcgcggg atcgtcgatc gcgcccgtag
     4141 gtggacgggc caccgacgtc acgggcgcat gccccagcag gctctcggtg gtctcggcac
     4201 cgacgacacg ttgatcctgc aggtcgtccg ccaatccgcc ggagaccatg gccggcagca
     4261 acggcgcgga gaagggcatc gcggacagct gcacggcgtt acgttccacg accttgcgga
     4321 gcgtcttcag cacggtcgcc gcgttggtgg ccggcgcttc atcggcgggt acggtcgtgc
     4381 cgtcgcttcg ctcgtagcca tcggccatgc ggtcgagctg atcgagcagg agcggctcga
     4441 cgaccaggtc gatcgtggag cgtaggtcgg ggtcctcgtc catccgaagg agggcggccg
     4501 cctgcatggc gagcgccccc tccggtgcga ccgccgcctc gagagatgag tcggacagcc
     4561 tgccatcagg accgaaggcg atcggaccgc tgacctcggt ccaccacgcg agctggagcg
     4621 gctgctccgg agctcgcacc aggtggatca ggggcgtgtt cagcgtggcg atcggtgcat
     4681 cagccgagcg gatggatacc tgcgatgggt acacgcccga atcctgcgtc ggatcggcga
     4741 tctccgggat gctcgtcatg tcgatcttca cggtgaagag ccgctcctga cctggttcga
     4801 gcgtgccttc ctgaggaagg gaggtggtgg atatcggcgc cagcccaggg ccgtcgatga
     4861 gcgcctgttc gtagacgaat ctcgagatga tcttgggtcc gatggagaac cccagggaga
     4921 ggtcgccgat cgccgcgtcc cccgcgttga ccgcgcgtac gaccagttcc agctcgggct
     4981 cgctgttggt cgtgaccggc gtctgggaga acagggtgag ggagacgagg ggctcctgcg
     5041 cctgcacgcc gaccgcgggc aacaggcacg cagcgacgat gagggagagc gcgaaggatg
     5101 cgcgcagggc gcggtggatg ctcatgccgg cggcctcatc gtagcggccg tgccgctagc
     5161 tcccgagcag ctcgatggcc tcggtggtgc cgtaggccag cgcgacgacc agcaggaacg
     5221 acccgacgag caggcgcagc gtgcgctcct tcgtacccag cgcgatatgc gctccgagcc
     5281 gcgccccggg cacggcgccc agcgtcacca ccgcgaagat cgcccagtcg atgtggccga
     5341 gcgcggcatg cacgatcgtt ccggggatca ccagcgccac gatcgtgagc aggctggtcc
     5401 ccagcgcgcg cttcagcggc atccccagcc agcccaccag gaggggcacc atgatgagcc
     5461 cgccgccgat ccccagcaga ccgctgacga acccggccac caacccgatc ccgaggtaca
     5521 tggacggcgt cgtttcgaag cgcggtccct cgccggtgtc cacccgggct cgcgcgccgc
     5581 gcaggatgtc ggccgcctgc caggcgagca acaccgcggt gatcaccagc agcaggtggg
     5641 tgttcaccac atcggtgagc gccgcccccg cgatcgaacc cacgatcccc gggccgacca
     5701 tccacgccac cgcgcgcatg tcgatctcgc cggccttgcg gtaggtcatg gccccggtga
     5761 aggccgtggg caggatcacc ggcaacggcg tggcgagcgc gacgatgggg gaggtctgca
     5821 acagcacctg gatgcccggg gtcatcacga tgccaccacc caccccgaac aagccggaga
     5881 gcacgcctgc gaccaggccg atcaacacgc ccaggatcgc ttcgaagacg gtcatcgcag
     5941 gaggtcggcg gcgcgcccga ggacctcgcg ctcaccgcga taggcggcgc gcttgagggc
     6001 gcggtccagc gggaaccagc gcacatcctc catctcgtcg tcgcgatcgt cggggtcgcc
     6061 accggtgcat cgcatcagga agaagtgcac ggtcttgcgg atccgggtgt cctcccacac
     6121 gtagaagtag cgggtgtcgc cgagcgaggc ttcgatctcg gcgtcgtagc ccgtctcctc
     6181 cttgacctcg cggacggcgg cctgttcatg gctctcgcct tcctcgatcc cgcccttggc
     6241 cagaccccag gcgagttccc ctcggcgggt tcgccgcgag gcgagcacga cctcgatctc
     6301 ctcgccgtcg cgtcggtaga ccacgccgcc cgccgagatc tcacgctgca cgggcccctt
     6361 gccctggttg tggggccgcc cggccttctt ggtccgcttc ttcttgcctt tcgacggctg
     6421 tttgcccttg gcgcgtggtt gctgctcggc aggttccttc gcgttcgggt cggaggcttg
     6481 cgcctgggtg gtctgcgtgg cggcctggga gcccgggcga cgacgacgcc gtcggcggcg
     6541 cttgcgcttg ggcgcatcgc ctccgtcggg ggtggatccc tcgctcatcg cagggattct
     6601 aggcgggagg attctccgcg aaggacttag gtgtctccgg gggtcggccg atggttccac
     6661 aggaccgccg aacggcggtg gtccacgctg aagggatccc gtccggcatg gagatcgacg
     6721 aactgctccg gtacaccgtg gagcgaggtg cgagcgacct gcacctcaag gtcgggaacg
     6781 tgcccttcat cagggtggac ggcacgttga cgccgaccga tttcgacgtg ctcacctcca
     6841 ccgacaccga gtcgttcgcc atgttgctca tgtccgagca caagaagcgg gagttctccg
     6901 agaccagtga ggccgacctg gggaccacgc tcaccggcgt cggccgcttc cgggtcaacg
     6961 tgttccgcca gcgcggcgtg gtgggcttgg cgatccgtcg tgttcgcagc gaggtaccga
     7021 cgttcgaaga gctccggctt ccccccgtga tggaggcgct gggcgattca ccgcgagggc
     7081 tggtgttgat caccggcc
//
LOCUS       DATDBZ010000323         2829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2335801, whole genome shotgun sequence.
ACCESSION   DATDBZ010000323 DATDBZ010000000
VERSION     DATDBZ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2335801"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VIX62_07850"
     CDS             <1..419
                     /locus_tag="VIX62_07850"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY5860181.1"
                     /translation="MLAPLPFIVLGVIVNLGFPSFFEVGGPSATLRIVSIVVLIPGVT
                     VWLWSAVLILRKVPRGELITTGPFAWVKHPIYTSVALLVIPCAGFLLNTWLGALIGVV
                     LYIASRRYAPEEEARLAESFGPAWDEYCNTVTIRWL"
     gene            560..799
                     /locus_tag="VIX62_07855"
     CDS             560..799
                     /locus_tag="VIX62_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860182.1"
                     /translation="MKRVAVVLGGAMAFVTTLVGTAFAQTTEAPVPTTMVEGAGGGAA
                     FTGGDTATAAFVAVALVTVGLVALLVARHHTSAAS"
     gene            874..1137
                     /locus_tag="VIX62_07860"
     CDS             874..1137
                     /locus_tag="VIX62_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860183.1"
                     /translation="MSRTTGTSILVLGIVLSVVGAIMYWAVTAKADGFDINAAGLILF
                     WVGVVTAVIGLVLTFTGNRSTTVRRGDALDGGRYEYEEEHRSI"
     gene            1217..1402
                     /locus_tag="VIX62_07865"
     CDS             1217..1402
                     /locus_tag="VIX62_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860184.1"
                     /translation="MGNSNAGAAAGGGAIYGLGIFGAFVYYWQAADQFWEYILAFIAG
                     LFWPAFMVYEVFKTLGA"
     gene            complement(1412..1582)
                     /locus_tag="VIX62_07870"
     CDS             complement(1412..1582)
                     /locus_tag="VIX62_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860185.1"
                     /translation="MTDPVPFCEQCGRPGPSLSGMHVGDDGLVRWTLYRCGHVHTQVV
                     LEDAPQPQPIGS"
     gene            complement(1696..>2829)
                     /locus_tag="VIX62_07875"
     CDS             complement(1696..>2829)
                     /locus_tag="VIX62_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY5860186.1"
                     /translation="RRHDLDWLRVIAVFLLLPFHSARVFDPFEDFYVHSAATSEALFW
                     SVIAFIGQWQMELLFVLAGAASWYAFGRRNAHEYRIERVKRLLVPFLFGLVVIVPIQS
                     YLAIVWRDGSASIPAFTTDYWTMQGEALGYDGGFTPGHLWFILFLFVYSMVAAPLFAR
                     WRSRTGPRRWVPWYLFLMPLVMFLSDAVSWPEDGPQNLFYSFAMFVAGFLLVSEPRLE
                     AAIDDAWRWLAPVAGVMVAAQMWLWISGTGDRWDAGVMSVVLDVWSATVTWVSVLALL
                     ALGRRFLSFETSFLRWGNQAAYPIYLLHQSVIIVVAYALLTAFTMPALPGFVLVSVGS
                     LLATVGVYESVVKRTNLTRFLFGMKPLAAREPRAHPARIPAMR"
BASE COUNT          497 a          928 c          956 g          448 t
ORIGIN      
        1 tcatgctcgc gccgctgccg ttcatcgtcc tcggcgtgat cgtgaacctt ggcttcccgt
       61 cgttcttcga ggtcggcggt ccgtccgcca cgttgaggat cgtctcgatc gtggtcttga
      121 tacctggggt cacggtctgg ctgtggtcgg cggtactgat cctgaggaag gtgccccgag
      181 gtgagctgat caccaccggg ccgttcgcat gggtgaagca cccgatctac acgagcgtgg
      241 cgctcctcgt gatcccgtgt gccggcttcc tgctgaacac gtggttgggc gcgctgatcg
      301 gggtcgtgct gtacatcgcg tctcggcggt acgcacctga ggaagaggcg cggctggctg
      361 agagcttcgg cccagcctgg gatgagtact gcaacacggt cacgatccgg tggctctagg
      421 ttgacacggg catgaggtcg gggctcacga gtcccgctct cgcaggggct tccgcctcat
      481 caccgatgag gcccacgacc tacgcttggc gcatatcgac acggacaccc gatccgtgcc
      541 ccgctggaag gggagatcga tgaagagagt ggctgtggtc ttgggcggcg cgatggcctt
      601 cgtgaccacg ctcgtcggca ccgccttcgc ccagacgacc gaagcgcccg tgccgaccac
      661 catggtcgaa ggggctggcg ggggtgctgc tttcaccggc ggcgacaccg ccaccgccgc
      721 gttcgtggcg gtcgcgctgg tcaccgtggg gctcgtcgcg ctgctcgtgg cgcggcatca
      781 caccagcgcc gcgtcctagt ccgaccaacc tggtccgggt ttggcgtgca tgccttccgg
      841 ggtagatccg tcgcacctcg gaaggaggac cagatgagtc gtaccaccgg aacgagcatc
      901 ttggtgctgg gcatcgtgct gagcgtcgtg ggcgcgatca tgtactgggc cgtcacggcc
      961 aaggccgatg ggttcgatat caacgccgcc ggcctcatcc tgttctgggt gggtgtcgtc
     1021 accgccgtca tcgggctcgt gctgacgttc acggggaacc gctccacgac cgtgcgtcgc
     1081 ggggatgccc tcgacggcgg ccgctacgag tacgaggaag agcaccgctc gatctgaccc
     1141 gagacgggcg ctcacgtgtg ccatcattcg cttggacggg cctcgcccgg cccgtcgaag
     1201 cgaaaggtgg gtggccatgg gcaacagcaa tgcgggggcg gcggctggtg gaggcgcgat
     1261 ctacgggctc ggcatcttcg gagccttcgt ctactactgg caggcggccg accagttctg
     1321 ggagtacatc cttgccttca tcgcgggact gttctggcct gcattcatgg tctacgaggt
     1381 cttcaagacc ctcggagcct gaaggagtcg cctaggaacc gatcggctgt ggctgcggtg
     1441 cgtcctcgag gacgacctgg gtgtgcacgt ggccgcaccg gtagagcgtc catcgcacga
     1501 ggccatcgtc gcccacgtgc atgccggaca acgacggtcc gggccggcca cactgctcgc
     1561 agaaggggac tgggtcggtc atgggcggag tctgccgaac gactgaccct gctggcatcg
     1621 gtccgacgac cactttcccg accccccggt acccgttcct gactaggact cctggaccgg
     1681 cagaggcgat cgcgttcacc gcatggcggg tatgcgcgca gggtgtgcgc gcggctcacg
     1741 cgccgcgagc ggcttcatgc cgaacaggaa cctcgtgagg ttcgtgcgct tcacgacgga
     1801 ctcgtagacc ccgaccgtcg cgagcaggga gccgacggag acgagcacga acccgggaag
     1861 ggccggcatc gtgaacgcgg tcagcagcgc gtacgcgacc acgatgatca cgctctgatg
     1921 cagcaggtag atcgggtagg cggcctggtt cccccatcgc aggaacgagg tctcgaacga
     1981 caggaagcgc ctcccgagcg cgagcagcgc gagcacgctc acccaggtga ccgtcgccga
     2041 ccagacgtcg agcacgaccg acatcacccc ggcgtcccag cggtcgccgg tccccgagat
     2101 ccagagccac atctgcgcgg cgaccatcac tcccgccacc ggcgcaagcc accgccacgc
     2161 gtcgtcgatc gcggcctcca ggcgcggttc cgacacgagc aggaacccgg cgacgaacat
     2221 cgcgaacgag tagaagaggt tctggggacc gtcctcgggc cacgacaccg cgtcgctgag
     2281 gaacatcacg agcggcatga ggaacagata ccagggaacc cagcgccgag gtcccgtgcg
     2341 tgaccgccag cgggcgaaca acggcgccgc caccatcgag tagacgaaca ggaagaggat
     2401 gaaccacagg tggccgggcg tgaacccgcc gtcgtagccg agagcctcgc cctgcatcgt
     2461 ccagtagtcg gtcgtgaagg cggggatcga cgcactgccg tcccgccaca cgatcgccag
     2521 gtaggactgg atcggcacga tcacgaccag accgaacagg aacgggacga gcaaccgctt
     2581 cacgcgctcg atgcggtact cgtgggcgtt gcgccgcccg aacgcgtacc acgatgccgc
     2641 cccggcgagc acgaacaaaa gttccatctg ccactgcccg atgaatgcga tcacggacca
     2701 gaacaacgcc tcgctcgtcg cggccgagtg cacgtagaag tcctcgaacg gatcgaacac
     2761 gcgcgcggag tggaacggga gcagcaggaa caccgcgatc actcggagcc agtcgaggtc
     2821 atgtcgccg
//
LOCUS       DATDBZ010000324         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_511757, whole genome shotgun sequence.
ACCESSION   DATDBZ010000324 DATDBZ010000000
VERSION     DATDBZ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511757"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1163)
                     /locus_tag="VIX62_07880"
     CDS             complement(72..1163)
                     /locus_tag="VIX62_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860187.1"
                     /translation="MFKYLEGVVSALKLLQLKFTVVWVTALLALNSFVTKSRMSHENG
                     IVTRGKLRIVDEPTFPRNEFFTPGTEFPCRMRFGAATWKDDAKMVIRGAGLKLADDRQ
                     TSPLDLLLNSGSLAIFWSARTFIGFMRGTMAGRGKNWTPYLGQHPMALAGGQDGMRRD
                     PTSIGSIVYHSQTCFGLIDLDGNYHYVRYRLWPLSYDPDGLPTPDDLPHPWFQNPLPD
                     ETRNRNYLKDEARERLAGGTIEFVLQLQARRKPPGPDPEWVTAQYVWDDSVHPWHDVA
                     HIVLDEALDYTESMLTAFDLANHPDCLPVPRGRSIDDPHSLSDLRLAGRWTMRARLLS
                     YRLRGMPAEFGDSRRDPDWIAVPPMPDPP"
     gene            complement(1245..2183)
                     /locus_tag="VIX62_07885"
     CDS             complement(1245..2183)
                     /locus_tag="VIX62_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860188.1"
                     /translation="MGDVWSALVDIATNESAYRNATIAAVVLLFAASGELVAEKAGSI
                     NISLEAMMLGGAFAAAVAVDLTGSTWVALLMAALAGLLVASVQANTSHRLPADQFVVG
                     LVLNVLVLGLVGFLASEIEPQSAVVSRVRVPVLAAVPLVGPALFDQPWVLYLVYPLVP
                     AIWWLLYRTRWGLEVRAIGEDPNASDVSGIHVNKLRRQTIYLAGLTAGLGGGYFLFAR
                     SGRFEDSLIGGRGYIAIAAVIFGGWTVKGTVGGCILFGLVGSFVLTIPSLGYNSIDPA
                     FLAMAPSVVTIVAMAVFAKRVRQPSALARPFIRGLK"
     gene            complement(2183..>2401)
                     /locus_tag="VIX62_07890"
     CDS             complement(2183..>2401)
                     /locus_tag="VIX62_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860189.1"
                     /translation="ALVARQRVLVVIFVAFVFGSLRTGSGFLRATGVSGRIADVVQGL
                     LVLALLLPPAILYVRERRRALSVTSSRV"
BASE COUNT          418 a          793 c          845 g          345 t
ORIGIN      
        1 gcgtcgatcg ccgcctcgac aagtgaccac ttgggcggcg cggtcagggt ggagtggtca
       61 ggacggagca gtcagggcgg atcgggcatc ggcggcacgg cgatccagtc ggggtcacgc
      121 cggctgtcgc cgaactcggc cggcatgccc cgcagccggt agctgagcaa ccgagcccgc
      181 atggtccacc ggccggcgag gcgcaggtcg ctcagcgagt gcgggtcgtc gatcgagcgg
      241 ccgcgtggga ccggtaggca gtcggggtgg ttcgccaggt cgaaggcggt gagcatgctc
      301 tcggtgtagt cgagtgcttc gtcgaggacg atgtgagcga cgtcgtgcca cgggtgaacg
      361 gagtcatccc agacgtactg cgccgtcacc cattccgggt cggggcccgg cggcttgcgc
      421 cgtgcctgca gctggagcac gaactcgatg gtgcccccgg cgagccgctc gcgggcctcg
      481 tccttcaggt agttgcggtt gcgcgtctcg tccggcagcg ggttctggaa ccacgggtgg
      541 ggcaggtcgt ccggcgtcgg cagaccgtcc gggtcgtacg acagcggcca cagccggtat
      601 cgcacgtagt ggtagttccc gtcgaggtcg atcagaccga agcaggtctg ggagtggtac
      661 acgatcgacc cgatcgaggt cggatctcgc cgcatgccgt cctgcccacc ggcgagggcc
      721 atcgggtgct ggccgaggta cggcgtccag ttcttccctc ggccggccat ggtgccgcgc
      781 atgaagccga tgaaggtgcg ggcgctccag aagatcgcca gcgatccact gttgagcagg
      841 aggtcgagcg gcgatgtctg ccgatcgtcg gcgagcttca gcccggcgcc gcgaatcacc
      901 atcttggcgt cgtctttcca cgtagcggcc ccgaacctca ttcggcacgg gaactcggtg
      961 cccggcgtga agaactcgtt gcggggaaac gtcggttcgt cgacgatgcg cagcttgcct
     1021 cgcgtcacga tgccgttctc gtgcgacatc cgcgacttcg tgacgaagct gttgagcgcc
     1081 aagagcgcgg tgacccagac gaccgtgaac ttcagctgca gcagcttcag cgcgctgacg
     1141 acgccctcga gatatttgaa catggtgcct tccgctcgga cggaccggcc gatgataggg
     1201 cgcgctcgcg ctggtggctc aacaaacggg gggaccgtgg ggcgtcactt caggccgcgg
     1261 atgaacggtc tcgcgagtgc ggatggttgt cgcacccgtt tcgcgaacac ggccatcgcc
     1321 acgatcgtca ccacgctcgg ggccatcgcc aggaaggccg ggtcgatgct gttgtaaccg
     1381 agcgagggaa tcgtcaggac gaacgagccg acgagaccga acaggatgca gccgccgacc
     1441 gtgcccttca ccgtccaccc gccgaagatc accgcagcga tggcgatgta cccacggcca
     1501 ccgatcaacg agtcctcgaa ccgccccgac cgggcgaaga ggaagtagcc cccgccgaga
     1561 ccggccgtga gaccggcgag gtagatcgtc tggcggcgga gcttgttgac gtggatgccg
     1621 gagacgtcgg acgcgttggg gtcttcaccg atcgcccgca cctcgagccc ccacctcgtc
     1681 cggtagagca gccaccagat cgccggcacg agcgggtaca ccaagtagag cacccacggc
     1741 tgatcgaaca acgcgggacc gacgaggggc acggctgcga gcacgggcac gcgcacgcgg
     1801 ctgacgacgg cggactgcgg ttcgatctcg ctggcgagga agccgacgag tccgagcacg
     1861 agcacgttga gcaccaggcc gacgacgaac tggtcggcgg gcaggcggtg actggtgttc
     1921 gcctgcaccg acgccaccag caggccggcg agcgccgcca tcagcagcgc cacccacgtc
     1981 gagcccgtga gatcgaccgc cacggccgct gcgaatgcgc ccccgagcat catcgcctcg
     2041 agcgagatgt tgatcgaccc cgccttctcg gcgacgagct cacctgatgc cgcgaacagc
     2101 agcacgacgg cggcgatcgt cgcgttgcgg tacgcgctct cgttcgtcgc gatgtcgacg
     2161 agggccgacc agacgtcgcc catcacaccc tcgagctcgt gacggacagc gcgcggcggc
     2221 gctcgcggac gtagaggatc gccggaggca acaacagcgc gaggacgagc agtccctgca
     2281 ccacgtcggc gatgcgtccg gagacgccgg tggcccgcag gaagcctgat ccggtccgga
     2341 ggctgccgaa cacgaacgcc acgaagatca ccacgagcac ccgctggcgg gcgaccagcg
     2401 c
//
LOCUS       DATDBZ010000325         2613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_321255, whole genome shotgun sequence.
ACCESSION   DATDBZ010000325 DATDBZ010000000
VERSION     DATDBZ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321255"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VIX62_07895"
     CDS             <1..225
                     /locus_tag="VIX62_07895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY5860190.1"
                     /translation="WSPLAAVAASALLRASYHLYQGWGGFAGNLAMGVLFGVLFIRWR
                     RTWPFVIAHCLLDVAAGLGYVFFRDHLPGF"
     gene            296..838
                     /locus_tag="VIX62_07900"
     CDS             296..838
                     /locus_tag="VIX62_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY5860191.1"
                     /translation="MSSVLDYLRGRGVPFLVLPDPDATHPEETAERHSVPTDELVRTV
                     VMSNRFGLALMVVPWHRQLDLALARRAMNDPNAQRALDQELEEKMPEFEVDAWPPLGL
                     FLLMPTFVDREVADREQVVFPAGKPGTLVCIQTEELFRDDPVVITSLTSETFAAEPVG
                     TRRGNLWAVRNEPQPAANTD"
     gene            complement(968..>2613)
                     /locus_tag="VIX62_07905"
     CDS             complement(968..>2613)
                     /locus_tag="VIX62_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY5860192.1"
                     /translation="SIQLAMERPPALKAIIPIFATDDRFGDDVHYFGGAVKALDLVDY
                     PTYMVAMNALPPVPSIFGDGWREEWERRVLETEPWLLTWRAHQRLDDYWKFGSLRFDI
                     GAIECPTMIIAGWADGYRNNTFRTFEALTCPKRMIIGPWSHASTDTCRPGPNHDLIPE
                     HLKWWDHWLKGNDNGVESEPPIMVYVQRSTLPAPDRAQVRGAWRYEPTWPAERLSPTT
                     LALAKAESDRDGDGPDRLEVRGDVGWTAWISCAGAMPWGQSADQRPDEIHSLTYTWEP
                     LEDELEIMGQARLSVRVTSSAPVAYLSAKLCDVFPDGTSSMVTRGMLNLTCRDSREDP
                     TALEPGTTYDIELELEAMSWVFEAGHRIRLDLAGSDWPNAWSPPEPVTLTIERATATL
                     ELPVLDGPGPVAEPPLVPPPNRVLANASPKELPKDDPEKGWVVWDVAHHLLRHETVAS
                     AGSMGNYDADPGSGTPSFQELYGGEVTVSTDDPGIASVTSRGAFDLRFPEATCSSRVD
                     ITVTSDRDHYDVAIDLRVSEDGEERWRRRWDQRFPRDLA"
BASE COUNT          385 a          890 c          890 g          448 t
ORIGIN      
        1 tggtcaccgc tcgccgccgt cgcggccagc gccctgctgc gagcgtccta ccacctgtat
       61 caaggctggg gtgggttcgc cggcaacttg gccatgggtg tgctgttcgg cgtgttgttc
      121 atccgctggc ggcgcacctg gccgttcgtg atcgcgcact gcctgctcga tgtcgccgcc
      181 ggcctcggct atgtcttctt ccgggatcat ctccccggat tctgagccga aaggaggatt
      241 ccgttcccga tggcttgctc gctacgatga gccgtcgtcg agcggaggag gggcgttgtc
      301 gtcggtactt gattacctgc gtgggcgcgg ggtgccgttc ctggtcctgc cggaccccga
      361 tgccacccat cccgaggaga cggccgaacg ccacagcgtc cccacggacg agctcgtccg
      421 cacggtcgtg atgtcgaacc ggttcggcct cgcgttgatg gtggtgccgt ggcaccgtca
      481 gctcgacctg gcgctggcgc gccgcgcgat gaacgacccg aacgcccagc gagcgctgga
      541 ccaagagctc gaggagaaga tgcccgagtt cgaggtcgac gcgtggccgc cgctcggcct
      601 gttcctgctg atgccgacgt tcgtcgatcg cgaggtcgcc gatcgtgagc aggtcgtgtt
      661 cccggcggga aagcccggaa cgctcgtgtg catccagacc gaggagctct tccgcgacga
      721 tcccgtcgtg atcacctcgc tcacctcaga gacgttcgcg gccgagcccg tcggcacgcg
      781 gcggggcaac ctctgggcgg ttcgcaacga gccgcagccc gccgccaaca ccgactgatt
      841 gtcgtttcgc tcagggtccc gtaccgtaga tggcgggata tcccatcaag accgaagccc
      901 ggccagcgcg ccgggacttc gtgtttctag ggtcggttcg gccccgaggg agcccgggaa
      961 cccccgatca ggccaggtcg cgggggaacc gctgatccca acggcgccgc caccgctcct
     1021 cgccgtcttc actcacccga aggtcgatcg ccacgtcgta gtggtctcga tcgctagtca
     1081 ccgtgatgtc cacccgtgag gaacacgtcg cttcggggaa ccgcagatcg aaggcacccc
     1141 ggctcgttac tgatgcgatc ccaggatcgt cggtcgagac cgtgacctcg ccgccgtaca
     1201 gctcctggaa cgacggtgtc ccggaccccg gatcggcgtc gtagttgccc atgctcccgg
     1261 cgctggcgac ggtctcgtgc cgcagcagat ggtgcgccac gtcccacacg acccacccct
     1321 tctccgggtc atccttgggg agttccttgg gagaggcgtt ggccaggacc cggttgggcg
     1381 gcgggaccag cggtggctcg gcgacgggac cgggcccgtc cagcaccggc agctccagcg
     1441 tcgcggtggc ccgctcgatc gtgagcgtca ccggttcggg gggcgaccac gcgttcggcc
     1501 aatcgctccc ggccaggtcc agccggatgc ggtgaccggc ctcgaacacc cacgacatcg
     1561 cctcgagctc gagctcgatg tcgtacgtgg tgccgggctc gagagccgtc ggatcctcgc
     1621 gcgagtcgcg gcacgtgagg ttgagcatgc cgcgcgtgac catcgaggac gtgccgtcgg
     1681 ggaacacgtc gcacagcttg gccgacaggt aggccaccgg cgcgctcgac gtgacgcgga
     1741 ccgacaaccg ggcctgcccc atgatctcca gctcgtcttc cagcggttcc caggtgtagg
     1801 tgagggaatg gatctcgtcc ggtcgttgat cggccgactg gccccagggc atcgcgcccg
     1861 cgcacgagat ccaggcggtc cagcccacgt ccccgcgcac ctcgagccgg tctggtccat
     1921 cgccgtcgcg gtcggactcc gccttcgcca gggccaacgt cgtggggctg agacgctcgg
     1981 cgggccaggt cggttcgtac cgccacgcgc cgcgcacctg ggcgcggtcc ggcgccggaa
     2041 gcgtagagcg ctgcacgtac accatgatcg gcggttcgga ctcgacgccg ttgtcgttgc
     2101 ccttcaacca gtgatcccac cacttgaggt gttcggggat gaggtcgtgg ttcggaccgg
     2161 gccggcacgt gtcggtggag gcgtgcgacc acggcccgat gatcatgcgc ttggggcagg
     2221 tcaacgcctc gaacgtgcgg aaggtgttgt tgcgatagcc gtcggcccag cccgcgatga
     2281 tcatcgtggg acactcgatc gctccgatgt cgaaccggag cgaaccgaac ttccagtagt
     2341 cgtccagtcg ctggtgcgcg cgccaggtga gcagccaggg ctcggtctcg agcacgcgac
     2401 gttcccattc ctcacgccag ccgtcgccga agatcgacgg caccgggggc agcgcgttca
     2461 tcgccaccat gtaggtgggg tagtcgacga ggtcgagcgc cttcaccgcg ccgccgaagt
     2521 agtgcacgtc atcgccgaac cggtcgtccg tggcgaagat cgggatgatc gccttcaggg
     2581 cgggcggccg ttccatcgcg agttggatcg agt
//
LOCUS       DATDBZ010000326         2435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_730951, whole genome shotgun sequence.
ACCESSION   DATDBZ010000326 DATDBZ010000000
VERSION     DATDBZ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730951"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VIX62_07910"
     CDS             <1..433
                     /locus_tag="VIX62_07910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860193.1"
                     /translation="DDGHLRLILLEIQGRPAAFDLSVGTEGYTFSYQTGFDPVFARLR
                     VGQVVLAQMIARAIDRGDRILDLGRGDEEYKAHWRATPMALDSAQVAQRRPTAMARFL
                     AVAAFDRFARSRAGQVAKEARRRGMALAISRSSGDDAAGRT"
     gene            490..1803
                     /locus_tag="VIX62_07915"
     CDS             490..1803
                     /locus_tag="VIX62_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY5860194.1"
                     /translation="MTTPDRSLAASTVLSMGAAATEIIGSFVVGWITARQLGPEGKGE
                     YTAIITWVLVIAWLCSVGLRDSVAFFQSRSPERSPEIFTTSLLYTVVLAAIGIGVAQL
                     LAPLAFRAQSDDVIAIARVAFFGIFLIVAFQTVEGLLSGQHRFVLLSATQALSSVGAA
                     VIMGLFALFGGMSVGEALIGKLGIYLLCTFGALILVLARVRPGRPSQPLARDMFGYGS
                     KLTFEGLGQLGTTQLDLVILPAVLVASEIGFYAVATSTATVIYLIFGRLGQIVLPVAA
                     RRDAASAVEFTARVTRLVLVGAAAAALALALIAQPLFVTIYGSEFDESLIPLLILLPG
                     SVAWSAYRIVASGLQGLNRPGQTSRAQLYATVVTLVGLALTLRPWGIRGAALTSSIAY
                     TTAFVACLRYLAAAGDISVRSMLRPRLFMTDVVSILRKGRRGLQS"
     gene            1823..>2435
                     /locus_tag="VIX62_07920"
     CDS             1823..>2435
                     /locus_tag="VIX62_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY5860195.1"
                     /translation="MGGATSGPSVAQRLLHDASGKARELMVRSPRVRRLVYSVRNRRL
                     FADLAQHDRMLADSVRIEAYYEALSAHIRPGDVVVDLGTGSGVLGFFAARLGAARVHA
                     IEHGPIIEAAQAVALANGIDNVVFHRVHSSDFELPERADVILHEQIGEAMFDERVVEN
                     VAELRDRILKPGGRIFPAHLRLFIEPVEVRADTRAPFAWEQRLH"
BASE COUNT          338 a          833 c          825 g          439 t
ORIGIN      
        1 cgacgacggc cacctccggt tgatactcct cgagatccaa ggccgcccgg cggcgttcga
       61 tctctctgtg ggaaccgagg gatacacgtt cagctaccag accggcttcg atccggtgtt
      121 cgcgcgactc cgcgttgggc aggtggtcct cgcccaaatg atcgcccgcg ccatcgatcg
      181 gggcgaccgg atcctcgatc tcggtcgggg tgacgaggag tacaaggcgc attggcgggc
      241 gacgccgatg gcgcttgatt cggcgcaggt cgcccaacgc cgtcccaccg cgatggctcg
      301 attcctggcg gtggctgcgt tcgaccggtt tgcgaggagc agggcgggtc aggtcgcgaa
      361 agaggcgcgt cgtcgcggta tggcgttggc gatctcgcgg tcgtcgggcg acgacgccgc
      421 gggacgtaca tgaacgcgag cccgccgacg tcggcgtctg catcgtcgtc cccatccgca
      481 acgccggtcg tgacgacacc cgaccgctca ctggcggcgt ccacggtgct ctcgatgggt
      541 gcggccgcca ccgagatcat cggctcgttc gtcgtcggct ggatcaccgc ccgccagttg
      601 ggaccagagg gcaagggcga gtacaccgcg atcatcacgt gggtgctcgt catcgcgtgg
      661 ctctgctcgg tggggctgcg cgattccgtg gccttcttcc agagccgcag tcccgaacgc
      721 tcgccggaga tcttcacgac atcgttgctc tacaccgtgg tcctcgccgc gatcggcatc
      781 ggtgtggccc aactgctcgc ccccttggcg ttccgggcgc agagcgacga tgtgatcgcc
      841 atcgcccgag tggccttctt cggcatcttc ctgatcgtgg cgttccagac cgtagaaggg
      901 ctcctctccg gccagcaccg cttcgtcctc ctcagcgcca cccaggcgct ctccagcgtc
      961 ggcgccgcgg tgatcatggg gctgttcgcg ttgttcggtg gcatgtcggt cggtgaggcg
     1021 ctcatcggca agctcggcat ctacctgttg tgcacgttcg gcgcgctgat cctcgtgctc
     1081 gcccgcgttc ggccagggcg accatcgcag ccactggcgc gcgacatgtt cgggtacggc
     1141 tccaagctca cgttcgaggg gctcggacag ctgggcacga cgcagctcga cctcgtcatc
     1201 ctgccggccg tgctcgtcgc cagcgagatc ggcttctacg ccgtcgccac cagcacggcc
     1261 acggtgatct acctgatctt cgggcgcctc ggccagatcg tgctgccggt ggcggcgcgc
     1321 cgcgacgccg ccagcgcggt cgagttcacc gcccgagtca cccgcctcgt cctggtcggc
     1381 gcggccgccg ccgccctcgc gctcgcgctg atcgcccagc cgctgttcgt gaccatctac
     1441 gggagtgagt tcgacgagag tctcatccca ctgctcatcc tgctgccggg cagcgtcgcc
     1501 tggtcggcct accggatcgt ggcgtcgggt ctgcaggggc tgaatcgacc cggccagacg
     1561 agccgggccc agctctatgc gaccgtcgtg acgctcgtcg gcttggcgct gaccctgagg
     1621 ccgtggggga tcagaggcgc ggccctgacc tcgagcatcg cctacacgac ggccttcgtg
     1681 gcgtgtctcc gatacctggc cgctgccggt gacatcagtg tgcgctcgat gctccgtcct
     1741 cggctgttca tgaccgacgt cgtctcgatc ctgcgcaagg gtcgccgtgg tctgcagtcg
     1801 tgatgtgagt accatttctc cgatgggcgg ggcgacgagt ggaccgagtg tcgcgcagcg
     1861 gctcctgcac gatgcaagtg gcaaggcacg cgagctgatg gtgcgttcac cgcgggtgcg
     1921 acgactggtg tactcggtca ggaaccgccg gctcttcgcc gatctcgccc agcacgatcg
     1981 catgctggcc gactcggtgc ggatagaggc gtactacgaa gcgctgtcgg cccacatacg
     2041 ccccggcgat gtcgtggtcg acctcgggac cggatccggg gtgctcgggt tcttcgccgc
     2101 tcggctgggc gccgcacgag tccacgcgat cgagcacggg ccgatcatcg aggcggcaca
     2161 ggctgtggcg ctggcgaacg gcatcgacaa cgtcgtgttc caccgggtgc acagcagcga
     2221 cttcgaactg ccggagcggg ccgacgtgat cctgcacgag cagatcggcg aggcgatgtt
     2281 cgacgaacgc gtcgtcgaga acgtggcaga gctgcgcgac cgcatcctca agccgggcgg
     2341 gcgcatcttt cccgcgcacc tccgtctgtt catcgaaccc gtcgaggtgc gcgccgacac
     2401 gcgtgcgccg ttcgcatggg agcagcggct gcacg
//
LOCUS       DATDBZ010000327         7029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2336743, whole genome shotgun sequence.
ACCESSION   DATDBZ010000327 DATDBZ010000000
VERSION     DATDBZ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2336743"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VIX62_07925"
     CDS             complement(<1..1140)
                     /locus_tag="VIX62_07925"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY5860196.1"
                     /translation="MDVQGMLSAKQLEELATAGEIDTVLCMFTDLQGRFMGKRILPDF
                     FLEEILGAEGLHACLYLLAVDMEMEPLPGYEYASWETGYGDFRMVPDMATLRLCPWLE
                     KTAMVICDIYAEDEDVAVAVAPRQILKDQLAKAAKMGYTVKTGSELEFYLFKDGYDEL
                     AERGYRDVHPSSSYIMDYHMLQTTKDEWIIRQIRNHMRGAGIPIEFSKGEFGKGQHEI
                     NITYSDALSNADHHSIYKHGVKEIAALNGVSATFMAKWTMEEAGSSCHLHSSIWNAKG
                     TKSLTWSDEGVGNMTDEFRWYLGGLMATAREMAWMYAPFVNSYKRYQLGSWAPTAIVW
                     SRDNRTCGYRTVGEHKAFRVESRIPGADANPYLAFAATVAAGMWGI"
     gene            complement(1229..2881)
                     /locus_tag="VIX62_07930"
     CDS             complement(1229..2881)
                     /locus_tag="VIX62_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5860197.1"
                     /translation="MDEAARASTGTDATRKTDTERLHELGYAQELKRGMSGFSNFAVS
                     FTIISILSGCLTLFGFGMFVGGPASSAYGWLLVGVMVTFVGLAMAEVCSSYPTAGGLY
                     YWSAKLAKKNGPAWAWFTGWFNLLGQVAITAGIDFGLSAFICAFMGIAFDVEVTKVIL
                     IGTYTVVLAVHGLLNTFGVRLVAIFNNVSVWWHVVGVAIVVAMCIILPDSHTSLSTIF
                     SFSQEPSAPGEVAGFINATGFTKSPIGILGITAYVFLIGMLLAQYTITGYDASAHMTE
                     ETHDADVAGPNGIWKSIVISVFFGYVLLLAVWYAMQGQAGYDIAAAFQYPATGNIAAP
                     AGIFLESFGKSAAIFALIIVMGAQFFCGMASVTTNSRMIYAFSRDGAVPGHRWWHTIN
                     KRTRTPTNAIWLAAVLAWLLVAPAYWMGSIVAYFAVTAVGVIGLYIAYIIPIFLRRRA
                     GDAFQEGPWKLGKWSALVGWIAVIWVALIVIVLMLPQFNWGDGIKFIDVFNFTPVIVG
                     GVFIITGLWWVISAHKWFTGPKVMGTAEELAAIEADLELKHS"
     gene            complement(3019..3795)
                     /locus_tag="VIX62_07935"
     CDS             complement(3019..3795)
                     /locus_tag="VIX62_07935"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5860198.1"
                     /translation="MRLDGKVAVITGGGSGMGKIASELFASEGAKVVLTDVNDDAGEA
                     AAAGIVAGGGDAAYVHADVSKESEAEAMVRAAVERFGGLHILYNNAGVMLPDDGSVDT
                     TDERIWDITLGVNVKGVAFGCKFGVPAMIASGGGSIINVASFVAWLGAATSQTAYTAS
                     KGAVLAMTREIAVEYARKGVRCNALCPGPIETPLLMQLLSDEQKKQRRFVHIPMGRLG
                     QAEELARAALFLASDDSSYMTGASLIVDGGITAAYVTPED"
     gene            3859..4203
                     /locus_tag="VIX62_07940"
     CDS             3859..4203
                     /locus_tag="VIX62_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860199.1"
                     /translation="MKIVADDPTLSSIREGGGVVWVWLDPHGGLGGPALIYLDASMAP
                     PGQSRQTKRKRSARRSHRFTEFPADGFELWCDFGKFHPPEELHLVLRRFPKRLEAFWN
                     GAIFVTDDLPEG"
     gene            4258..5688
                     /locus_tag="VIX62_07945"
     CDS             4258..5688
                     /locus_tag="VIX62_07945"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5860200.1"
                     /translation="MAESLVMGGERVEAADGATLEVIEPAAGDAMGTVASAGPEDATR
                     AVDLAQRAFEDGAWPRMSARERGRLLTKASLVIRDRQEELATLEARNGGKPISSARAE
                     IDIVANVFEYWGGAANKITGETIPIPPPGIDLTLREPVGVCALITPWNFPAVIASWKI
                     APALACGNTAIVKPASQTPLTTLAIAEILYECGLPEGTLSVLPGPGSTTATALIADPR
                     VAKISFTGSTEVGVKVMQTAAEHIARVSLELGGKSANVVFADTDLDTCVAKSLWSVFD
                     NAGQDCCARSRMLVERPAYDGFVERFAAATDEIAVGQPLDEATQMGPLISSGQRQTSV
                     DYLALGVQEGARHVTGGEVPDQPGFFMRPAVLADVDNASRVAQEEIFGPVVSIIPFDT
                     EDEAIRMANDSPYGLSGSIWTRDLGRAIRVSKGVRTGNLSVNSSSSVHTEAPFGGYKQ
                     SGMGREMGMHAVNLFTEVKNIYFSQE"
     gene            5729..6268
                     /gene="greA"
                     /locus_tag="VIX62_07950"
     CDS             5729..6268
                     /gene="greA"
                     /locus_tag="VIX62_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY5860201.1"
                     /translation="MVELPDELRDRPVRPANQEPTATLTLDAYLRIKAELEELTTNGR
                     EQITEKIKIAREHGDLKENAEYHAAKDEQGLMESKIRKMQQMLRDPEIVEAPPVSDEV
                     GPGMLVTIRPLDEPDPDEETYLLAEHTEEKAPGARTVTTESPLGTALMGATKGQEVAY
                     QAPGGTVKYSVVSFEPHTG"
     gene            6289..6939
                     /locus_tag="VIX62_07955"
     CDS             6289..6939
                     /locus_tag="VIX62_07955"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY5860202.1"
                     /translation="MTSHERPEREAPRVRDMPAPRKNESAAGGFMQRTLAWPYRAILA
                     FLIWTGVRPWQLTLASPVFIVLSGWQIVVGNWAVAGVLLLIGGLCDVFDGSVARHRGE
                     SGRSGAFMDSVLDRVSDMILFSCLFWNLAERGETLEAGLALLTLIVSLSVSQIRAEAE
                     AVGVKLSEGLFQRLERFLALLVGLLIPGMMLPALILLAVLGVVTVLQRGFSALTQA"
BASE COUNT         1190 a         2342 c         2315 g         1182 t
ORIGIN      
        1 gatgccccac atgccggcgg ccaccgtggc ggcgaaggcc aggtaggggt tcgcgtccgc
       61 gccggggatc cgcgattcca cgcggaacgc cttgtgctca ccgacggtgc ggtagccgca
      121 ggtccggttg tcccggctcc acacgatcgc ggtcggcgcc cacgaaccca gttgatagcg
      181 cttgtaactg ttcacgaacg gcgcgtacat ccaggccatc tcgcgcgccg tcgccatgag
      241 ccctcccagg taccagcgga actcgtcggt catgttgccg acgccctcgt cgctccacgt
      301 gaggctcttc gtccccttgg cgttccagat cgatgagtgc agatggcacg aggaccccgc
      361 ctcctccatc gtccacttcg ccatgaacgt ggctgatacg ccgttgagcg ccgcgatctc
      421 cttcacgccg tgcttgtaga tcgagtgatg gtcggcgttc gacagggcat cggagtaggt
      481 gatgttgatc tcgtgctggc ccttgccgaa ctcgcccttg gagaattcga tcgggatgcc
      541 cgcgccacgc atgtggttgc ggatctggcg gatgatccat tcgtccttgg tggtctggag
      601 catgtggtaa tccatgatgt agctgctcga cggatgcacg tcgcggtacc cgcgctcggc
      661 cagttcgtcg tagccgtcct tgaacaggta gaactccagc tccgagccgg tcttgaccgt
      721 gtagcccatc ttcgcggcct tcgccagctg gtccttcagg atctggcgtg gcgccaccgc
      781 caccgcgacg tcctcatctt cggcgtagat gtcgcagatc accatggccg tcttctccag
      841 ccatgggcac aggcgcaagg tcgccatgtc gggcaccatg cggaagtcgc cgtagccggt
      901 ctcccagctc gcgtactcgt agccgggcag cggctccatc tccatgtcga cggcgagcag
      961 atagaggcac gcgtgcaggc cttcggcacc caggatctcc tccaggaaga agtcgggaag
     1021 gatccgcttg cccatgaacc ggccctgcag gtcggtgaac atgcacagca ccgtgtcgat
     1081 ctcgcccgcc gtcgccagtt cctcgagctg cttcgcgctg agcatgccct ggacgtccat
     1141 gtccttactc cttctccggc ctcctgcggt gaacgaacga ggcgcggcgg ccgtgtcggc
     1201 ccgccgcgcc cccttcgatc gtcgatgcct agctgtgctt cagctcgagg tcggcctcga
     1261 tcgcggccag ttcctcagct gtgcccatca ccttgggtcc ggtgaaccat ttgtgcgccg
     1321 agatgaccca ccacagtcct gtgatgatga acaccccgcc gacgatcacc ggtgtgaagt
     1381 tgaacacgtc gatgaacttg atgccgtcac cccagttgaa ctgtggcagc atcagcacga
     1441 tgacgatgag tgccacccag atcacggcga tccaacccac gagcgcgctc cacttgccca
     1501 gcttccacgg gccttcctgg aacgcgtcgc ccgctcgacg acgcaggaag atcgggatga
     1561 tgtaggcgat gtagagaccg attaccccga cggcggttac ggcgaagtag gcgacgatcg
     1621 agcccatcca ataggccggc gccaccagca accaggccag cacagccgcg agccagatcg
     1681 cgttggttgg ggtgcgggtc cgcttgttga tggtgtgcca ccagcgatga ccgggcaccg
     1741 cgccgtcgcg cgagaacgcg tagatcatgc gcgagttcgt cgtgaccgat gccatgccgc
     1801 agaagaactg ggcacccatc acgatgatca gggcgaagat cgcggcgctc ttgccgaacg
     1861 attccaggaa gatcccggcc ggtgcggcga tattccctgt ggctggatac tggaacgcag
     1921 ccgcaatgtc atagccggcc tgtccctgca tcgcgtacca cactgcgagc agcaggacgt
     1981 agccgaagaa caccgagatc acgatcgatt tccagatccc gttggggccg gcgacgtcgg
     2041 cgtcgtgcgt ctcctcggtc atgtgcgcgg aggcgtcgta gccggtgatc gtgtactgcg
     2101 cgagcagcat cccgatcaag aacacgtatg ccgtgatgcc caggatcccg atcggactct
     2161 tcgtgaatcc ggtcgcgttg atgaaccccg ccacctcgcc tggcgccgag ggttcctgac
     2221 tgaagctgaa gatcgtcgac agcgatgtgt gcgagtccgg caggatgatg cacatcgcca
     2281 ccacgatcgc cacgccgacc acgtgccacc aaacgctgac gttgttgaag attgccacca
     2341 accgcacgcc gaaggtgttg agcagcccgt ggaccgccag caccaccgtg taggtgccga
     2401 tcaggatgac cttcgtcacc tcgacgtcga acgcgatgcc catgaaggcg cagatgaaag
     2461 ccgagagccc gaagtcgatg cccgcggtga tcgcgacctg acccagcagg ttgaaccagc
     2521 ccgtgaacca ggcccacgcg ggcccgttct tcttggcgag cttcgcggac cagtagtaca
     2581 ggccgcccgc cgtcggatag ctcgagcaca cctcggccat ggccaacccg acgaaggtga
     2641 ccatgacgcc gacgaggagc catccgtagg cgctgctcgc gggacctccg acgaacatgc
     2701 cgaacccgaa cagggtcaga caccctgaca ggatcgagat gatcgtgaag ctcactgcga
     2761 agttcgagaa cccggacatg ccccgcttca actcctgggc gtagccgagt tcgtggagcc
     2821 gctcggtgtc ggtcttgcga gtggcatcgg tgccggtgct cgctcttgcg gcttcgtcca
     2881 tctactcccc ccttccctcc cgtggtgcag gtggcgagac gaaccgtgcg atcccgaacc
     2941 ggtcggaacc ctcgcgtatc agagaccttg ggggcgaacc cgtcaagtcg gaggttcgga
     3001 tggagctgcc cggaatgact agtcctcggg cgtgacgtac gcagcggtga tgcctccgtc
     3061 gacgatcaac gaggctccgg tcatgtagct cgagtcgtcg ctcgccagga acagcgcggc
     3121 cctcgcgagt tcttcggcct gccccagacg gcccatcggg atgtggacga atcgccgttg
     3181 cttcttctgc tcgtcggaca ggagctgcat cagcagcggg gtctcgatcg gtcccgggca
     3241 caacgcgtta cagcggacac ccttgcgcgc gtactccacc gcgatctccc gcgtcatcgc
     3301 caggaccgcg cccttggatg cggtataggc ggtctgcgag gtggcggcgc cgagccatgc
     3361 gacgaacgac gcgacgttga tgatggaccc gccgccgctg gcgatcatgg cgggcacccc
     3421 gaacttgcac ccgaaggcca cgcccttgac gttcacccct agggtgatgt cccagatgcg
     3481 ctcgtccgtg gtgtcgaccg aaccgtcatc gggcagcatc acgcccgcgt tgttgtagag
     3541 gatgtgtagg ccgccgaacc gctcaacggc cgcgcgcacc atcgcctcgg cttcggattc
     3601 cttcgagacg tcggcgtgga cgtaggcggc gtcgcccccg ccggcgacga tccccgcggc
     3661 ggcggcttcg cccgcatcgt cgttgacatc ggtgagcacg accttcgcgc cttcgctcgc
     3721 gaacagctcc gaggcgatct tgcccatgcc gcttccgccg ccggtgatca ccgcaacctt
     3781 gccgtccaac cgcatccggt tcctcctccg cctccgacgc cgcgcgaagc ctatccccat
     3841 cggggtagcg tgtgcgccgt gaagatcgtc gccgatgacc cgacgctgtc ctcgatccgc
     3901 gagggcggcg gcgtggtgtg ggtgtggctc gatccacacg gtggtctggg tggaccggcg
     3961 ctgatctatc tcgatgcctc gatggcacca ccgggccagt cgcgccagac caagcgcaag
     4021 cgcagcgcgc gtcgctcgca tcgattcacc gagttccccg ccgacgggtt cgagctgtgg
     4081 tgcgacttcg gcaagttcca tccgcccgag gaactgcacc tggtgttgcg gcggttccca
     4141 aaacggctgg aggcgttctg gaacggcgcc atcttcgtca ccgacgatct gcccgagggc
     4201 taggtcgcca ccgagaacaa gcatccgaga ccaacgtctg agaacaggag aacgtcgatg
     4261 gccgaatcgc tggtgatggg aggcgagcgg gtcgaggccg ccgacggcgc gaccctcgag
     4321 gtgatcgaac ccgcagcggg ggacgccatg ggaacggtcg cctcggcggg accagaggac
     4381 gccacgcgcg ctgtcgacct cgcccagcgg gcgttcgagg acggtgcgtg gccgcgcatg
     4441 agcgcacgag aacgtggccg gttgctgacg aaggcgtcgc tggtgatccg cgaccgccag
     4501 gaggaactcg ccacgctgga ggcccgcaac gggggcaagc cgatctcgtc ggcgcgcgcc
     4561 gagatcgaca tcgtcgccaa cgtcttcgag tactggggcg gggcggcgaa caagatcacc
     4621 ggcgagacga tcccgatccc gccgccgggg atcgacctca cgctgcgcga gcccgtcggc
     4681 gtgtgcgccc tcatcacccc gtggaacttc ccggcggtga tcgccagttg gaagatcgcc
     4741 cccgcgctgg cgtgcggcaa caccgcgatc gtgaagccgg cgagtcagac gccgctcacc
     4801 acgctggcga tcgccgagat cctctacgag tgcgggctcc ctgagggaac gctcagcgtg
     4861 ctgcccggcc ccggctcgac gacggcgacc gcgctcatcg ccgatccgcg cgtcgcgaag
     4921 atctcgttca cgggctccac cgaagtcggc gtgaaggtga tgcagaccgc ggccgagcac
     4981 atcgctcgcg tgtcgctgga actcggcggc aagtcggcca acgtggtgtt cgccgacacg
     5041 gatctggaca cgtgcgtcgc gaagtccctg tggtcggtgt tcgacaacgc gggccaggac
     5101 tgctgcgcgc gctcgaggat gttggtggag cgtcccgcct acgacgggtt cgtcgaacgg
     5161 ttcgcggcgg cgaccgacga gatcgcggta ggccagccgc tcgatgaagc cacccagatg
     5221 gggccgctga tctcttccgg acagcggcag acctcggtcg actacctcgc gctcggcgtc
     5281 caggagggcg cccggcatgt caccggcggc gaggtgcccg accaacccgg cttcttcatg
     5341 cgaccggctg tcctcgccga cgtcgacaac gcctcgcgcg tggcccagga ggagatcttc
     5401 gggccggtgg tcagcatcat cccgtttgac accgaggacg aggcgatccg catggcgaac
     5461 gactcgccgt acgggctctc ggggtcgatc tggacccgcg acctgggccg ggcgatccgc
     5521 gtgtccaagg gcgtgcgcac cggcaacctg agcgtgaact cgagcagctc ggtccacacc
     5581 gaggccccct tcgggggcta caagcagagc ggtatgggcc gggagatggg gatgcacgcc
     5641 gtcaacctgt tcaccgaggt gaagaacatc tacttcagcc aggagtgacg ccgcacggcg
     5701 ccggtggccc tctcggtaga ctccggacat ggttgaactt cccgacgagc tgcgtgaccg
     5761 cccagtccga cccgcgaacc aggagcccac ggcgaccctg accctggacg cctacctgcg
     5821 gatcaaggcc gaactggagg agctcaccac caacgggcgc gaacagatca ccgagaagat
     5881 caagatcgcg cgtgagcacg gcgacctcaa ggagaacgcc gaatatcacg cggccaagga
     5941 tgagcagggc ttgatggaat cgaagatccg gaagatgcag cagatgctgc gcgaccccga
     6001 gatcgtcgag gcgccgccgg tctccgacga ggtgggtccc ggcatgctcg tcacgatccg
     6061 gccgctcgac gagcctgacc ccgacgagga gacctatctg cttgccgagc acaccgagga
     6121 gaaggctccc ggtgcccgga ccgtcaccac ggaatccccg ctcggcaccg ccttgatggg
     6181 tgccacgaag gggcaggagg tcgcgtatca ggcgccgggc ggcacggtca agtactccgt
     6241 ggtctcgttc gagccgcaca ccggctagct acgcgatgcg atgaacccgt gacctcccac
     6301 gagcgaccgg agcgcgaagc cccgcgggtg cgggacatgc ccgctcctcg caagaacgag
     6361 agcgccgccg gcgggttcat gcagcgaacc ctcgcctggc cctaccgcgc gatcctggcg
     6421 ttcctgatct ggacaggggt acgtccctgg cagctcacgc tggcctcgcc ggtgttcatc
     6481 gtgctctcag gttggcagat cgtggtcggc aactgggccg tcgcgggcgt gctgctcctg
     6541 atcggaggcc tgtgtgacgt cttcgacggg tcggtggccc gtcaccgggg cgaatcgggg
     6601 cgctcgggtg cgttcatgga ctcggtgctc gaccgcgttt cggacatgat cctgttctcg
     6661 tgcctgttct ggaacctggc agagcggggt gagacgctcg aggcgggact cgcgctgctg
     6721 acgctgatcg tgtcgctgag cgtgtcgcag atccgggccg aggccgaagc cgtgggcgtg
     6781 aagctcagcg aggggctgtt ccaacggctc gaacgcttcc tggcgctcct ggtgggtctg
     6841 ttgatcccgg ggatgatgct gccggcgctg atcctgctcg cggtgctcgg cgtggtgacg
     6901 gtgttgcagc gcgggttctc ggcgctgacg caggcgtgag gagggtgcgg gcgatggact
     6961 tcgtgcgggt gggctcgctg ggtgagatcc ccgaaggtga ggcgcgcgcg ttcgacacgc
     7021 ccgcgggcc
//
LOCUS       DATDBZ010000328         2980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_512745, whole genome shotgun sequence.
ACCESSION   DATDBZ010000328 DATDBZ010000000
VERSION     DATDBZ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512745"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VIX62_07960"
     CDS             complement(<1..1039)
                     /locus_tag="VIX62_07960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013584869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5860203.1"
                     /translation="MTPSETPDDPRATEPDERRPLTVLIGADTFSPHVNGAARFAERL
                     AAGLVARGHHVHVMAPSAKHWARGSGVEVIEDQPMMLYRLPSWRWWPHDWLAFVLPWM
                     SKHHARRVLDTVQPDVVHIQSHIIIGRGLAREARTRGIPIIATNHVMAENILDFTTLP
                     HALNDVFLKLAWADAKRTFDMTRAVTTPTRKAADFLESTIDITGVIPISCGIDRSNYR
                     PDLTPRDANRILFVGRLTTEKHIDVLLKAVAQLDPALDVHVDLVGGGDQRKNLEALVH
                     SLGLDGHVTFLGHAAEEDLRTLYSRAGLFAIASVAELQSIATMEAMASGLPIVAANAV
                     ALPHLVHDGDNG"
     gene            1178..1768
                     /locus_tag="VIX62_07965"
     CDS             1178..1768
                     /locus_tag="VIX62_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860204.1"
                     /translation="MPRLQADADKTVWVAVTDSLGFGGRRHRKAAIRMLLSQANRAMG
                     AEERPGGGFMAWAMSQATPFLMRKAAGRVLVWVWKADPELLVVMAQVQEATAQLRAAR
                     ASMPMEYDDTETFRGTYLGVGEKLAIPLPADQRTPPFATYTWDTGSHFVAVTAVCSDR
                     ERFGTVIGAVDELARTLRISDDVALGESPNVLRIEP"
     gene            complement(1802..2932)
                     /locus_tag="VIX62_07970"
     CDS             complement(1802..2932)
                     /locus_tag="VIX62_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY5860205.1"
                     /translation="MSDPSPEIIPEPQAPIPPIPSVPERRGVLALIDRPLVIAFIATL
                     GVLGGLVLGSAIASITVILIYIVLALFISLGLDPIVRVLERRGVKRGGGIAIVFGAFA
                     VLALAFFIFVLPPILGQVGQFVREFPDAVVEAVNSEWFLALAPDLQDAVGAALAELSA
                     LLAKPETIAAVGGGALAVGIGIVNAITASFIVIALTLYFLSSLTGMKNALYDLAPAHS
                     RARLADMTERITASVGSSLIGSVTLSSINAAVVLVLHLVIGLPFAALMTFIAFVITLI
                     PLFGSVIFWLLATGVALFSSPAQAVTFFVAYLIYIQIESYVVSPRVMNRAVSIPAALV
                     LIGALVGGALAGVIGVLVALPIMASMLLILREIVVPKQSLKV"
BASE COUNT          535 a          962 c         1048 g          435 t
ORIGIN      
        1 atccgttatc gccgtcgtgc acaaggtgcg gcagcgcgac ggcgtttgcg gcgacgatcg
       61 gcagtcccga tgccatcgcc tccatcgtcg cgatcgactg cagctcggcg acagacgcga
      121 tcgcgaagag gccggcgcga ctgtataggg tgcgcagatc ctcctcggcc gcatggccga
      181 ggaacgtgac gtgaccgtcg agaccgagtg aatggaccag cgcctcgaga ttcttgcgct
      241 ggtcgccacc gccgacgagg tcgacgtgca cgtcgagggc cggatcgagc tgggcgacgg
      301 ccttcagcag cacgtcgatg tgcttctcgg tcgtcaggcg cccgacgaac aggatgcggt
      361 tcgcatcacg cggcgtcagg tcggggcggt agttcgaccg gtcgatcccg cagctgatcg
      421 ggatgacgcc ggtgatgtcg atcgtcgact cgaggaagtc cgccgctttg cgggtgggcg
      481 tggtcaccgc ccgcgtcatg tcgaacgtgc gcttggcgtc ggcccacgcg agcttgagga
      541 agacgtcgtt gagagcgtgc ggcagggtcg tgaagtccag gatgttctcg gccatcacat
      601 ggttggtggc gatgatcggg atgccgcgcg tgcgcgcctc gcgggccagg ccgcggccga
      661 tgatgatgtg cgattggatg tgcaccacgt cgggctgcac ggtatccagc acgcgacggg
      721 cgtggtgctt cgacatccat ggcaggacga acgcgagcca gtcgtgcggc caccagcgcc
      781 acgagggcaa ccggtagagc atcatgggct ggtcttcgat gacctcgaca ccgctgccgc
      841 gagcccagtg cttcgcactg ggtgccatca cgtgcacgtg gtgtccgcgg gcgaccaggc
      901 cggcggcgag gcgctcggcg aatcgcgctg cgccgttgac gtgcggtgag aacgtgtctg
      961 caccgatcag caccgtgagg ggtcggcgtt cgtccggctc agtggcgcgg ggatcgtcgg
     1021 gcgtctcgga cggggtcacg ggatgctggt tgcctatctc tgcgcggggg cgggtcgcac
     1081 gaggtcggtg catgtgcggt gccaactcta cccgagcacc ctccgggacc ctgagccgtc
     1141 caccgaacgc acacggtcga cctcgtaccc tggactcatg ccccgattgc aggccgatgc
     1201 cgacaagacc gtctgggtcg ccgtcacgga ctcgctcggg ttcggtgggc gacgccatcg
     1261 caaggcggcg atccgcatgc tgctcagtca agcgaaccgc gcgatgggcg ccgaggagcg
     1321 ccccggaggc ggattcatgg cgtgggcgat gagccaggca acgccgttcc tgatgcgcaa
     1381 ggctgccggt cgggtgctcg tctgggtgtg gaaggccgac cccgagctgc tggtcgtgat
     1441 ggcgcaggtg caggaggcga ccgcgcagct gcgcgccgcg cgagcaagca tgccgatgga
     1501 gtacgacgac accgagacgt tccgcggcac gtacctcggt gtcggcgaga agctcgcgat
     1561 ccctctgccg gccgatcagc gcacgccccc gttcgccacg tacacgtggg acaccggatc
     1621 gcacttcgtc gcggtcaccg ccgtgtgcag cgaccgcgag cgattcggca cggtgatcgg
     1681 cgcggtggac gagctcgccc gcacgctgcg catcagcgac gacgtcgccc tcggcgagtc
     1741 gccgaacgtc ctgcggatcg aaccctgacg cagacccgtc ggccggccgc cagacgcgct
     1801 gtcagacctt caggctctgc ttgggcacca cgatctcgcg gaggatgaga agcatcgacg
     1861 ccatgatcgg aagcgccacc agcacgccga tgacacccgc cagcgcgccg cccaccagcg
     1921 cgccgatgag cacgagggca gccgggatcg acaccgcccg gttcatcacg cgggggctca
     1981 ccacatacga ctcgatctgg atgtagatca ggtaggcgac gaagaacgtc accgcctgcg
     2041 ccggactcga gaagagcgcg accccggtcg cgagcagcca gaagatcacc gatccgaaca
     2101 gcggaatgag ggtgatgacg aaggcgatga acgtcatgag ggcggcgaac ggcaggccta
     2161 tcaccaggtg cagtaccaag accacggcgg cgttgatcga ggagagcgtc accgagccga
     2221 tgagcgagct gcccaccgaa gcggtgatcc gctcggtcat gtcggcgagc ctggcacggc
     2281 tgtgcgcggg agccaggtcg tagagcgcgt tcttcatccc ggtcagtgag gagaggaagt
     2341 acagcgtcag cgcgatcacg atgaagctcg ccgtgatcgc gttgacgatg ccgatgccga
     2401 ccgccagggc tccgccgccg actgcggcga tggtctcggg tttcgccagc agagcagaca
     2461 gttcggcaag ggccgctccg accgcgtcct gcagatccgg cgccagcgcc aggaaccact
     2521 cggagttcac cgcctccacg accgcatcgg ggaactccct cacgaattgg ccgacctgac
     2581 cgaggatcgg cggcagcacg aagatgaaga acgcaagcgc gagcacggcg aaggcgccga
     2641 acacgatcgc gatcccccca ccccgtttca ccccgcggcg ctccagcacc cgcacgatcg
     2701 gatcgagccc gagcgagatg aacagtgcca gcacgatgta gatgaggatg accgtgatcg
     2761 atgcgatcgc cgagcccagg acgagtccgc ccagcacacc gagagtcgcg atgaacgcga
     2821 tcacgagcgg gcgatcgatg agggcgagga cgcctcgccg ctcagggacc gacgggatgg
     2881 gcggaatcgg cgcctggggt tcagggatga tctctggcga ggggtctgac atgcccgaac
     2941 aatatcgccg acgcacccac tgcggtgtca tgccacaagg
//
LOCUS       DATDBZ010000329         2372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2773632, whole genome shotgun sequence.
ACCESSION   DATDBZ010000329 DATDBZ010000000
VERSION     DATDBZ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773632"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VIX62_07975"
     CDS             <1..210
                     /locus_tag="VIX62_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY5860206.1"
                     /translation="DRGGYAGPVGWLDAEGDGAWAIALRCAELDDDRATLFAGAGIVG
                     GSDPDAEVDETDRKFRAFLDSLRWG"
     gene            complement(247..414)
                     /locus_tag="VIX62_07980"
     CDS             complement(247..414)
                     /locus_tag="VIX62_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860207.1"
                     /translation="MERTAVIDTITSAATPHDISNAMCEARTWLSDHPEDEAVRVGIQ
                     QLARAERELRS"
     gene            complement(489..2261)
                     /gene="menD"
                     /locus_tag="VIX62_07985"
     CDS             complement(489..2261)
                     /gene="menD"
                     /locus_tag="VIX62_07985"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEY5860208.1"
                     /translation="MAEDMNLGDLSLACAWALVDGSVRGGVRDACLSPGSRSTPIALA
                     LARHPSIRLHVHLDERSSGFFAVGLANASRRPVIIACTSGTAAAEFLPAIVEASRSRI
                     PLLALTADRPPRLRGTGANQTIDQVGLYGGYVRECLDLPVPETPGQESWWAQSALQAL
                     EATRSDPVGPVHVNCPFDEPLTPSQDTTLPPPDDEHLELPRRPEAALTEEEADRLASM
                     VSGARGAVVIGGWPSALDGEASYWSQTLGWPVIAEPGSGARRPEESLAAGQALLGSSA
                     WVETHRPDVVIQVGATPTSRATQAFVGAAERLIVADRWHPDSDPERHASWRLAVDADA
                     LQQALERRRWRPAPDDWSSGWAETDTIARSGLDRFLDGIDEPFEPRIARDLAAWIPQP
                     GTLFVGNSTPIRDLDLAMAPRDGLRVMANRGASGIDGLVSTALGIASTGPYGGRADTP
                     PGPTVALLGDLSVLYDVGALLWAGRTGVDLVVVVVANGGGEIFSLLPQRELPEHRDLF
                     VTPHRIDLAPVCAAADVGYARLDQAAALTPALDHAVGAGGLQVVEVVVDPVRGLELRA
                     ELRATIEAALAVPAPPPPPHATER"
BASE COUNT          350 a          779 c          898 g          345 t
ORIGIN      
        1 gatcgaggtg gctacgccgg gccggtgggg tggctggacg ccgaaggaga cggggcgtgg
       61 gcgatcgcgc tccggtgcgc cgaactcgat gatgaccgcg ccacgttgtt cgcgggcgcc
      121 ggcatcgtcg ggggttccga ccccgatgca gaggtcgacg aaaccgaccg gaagttccgg
      181 gcgttcttgg attcgttgcg ctgggggtga ggggcgctcc gccgcggacg gcgggcgcga
      241 ccgtggttag ctgcggagct ctcgctcggc gcgtgccaat tgctggatcc cgacgcgcac
      301 ggcctcgtcc tccgggtggt cggagagcca ggtgcgggct tcacacatgg cgttggagat
      361 gtcgtgtggc gtggcggcgc tggtgatggt gtcgatgact gcggtgcgtt ccatgtatcc
      421 atgtgtacct gctgacggcg ccggtggtgg gtgtcaggcg atcacgtcac atggtcgtca
      481 cacgtaggtc aacgttccgt cgcatgaggc ggtggcgggg gtgccggaac ggccagcgct
      541 gcctcgatcg tggcgcgcag ctcggctcgc agctcgagcc cgcgaaccgg gtcgacgacg
      601 acctccacga cctggagacc gccggcaccg acggcgtgat ccagggcggg cgtgagggcg
      661 gccgcctgat cgagtcgcgc atagcccacg tcggcggccg cacacacggg ggccaggtcg
      721 atccggtgcg gcgtcacgaa caggtcccga tgctcgggca actcccgttg gggaagcagg
      781 gagaagatct ctccgccgcc gttggcgacg acgacgacga cgaggtcgac gccggttcgt
      841 cccgcccaca gcaaggcacc gacgtcgtac aggacggaca ggtcaccgag caacgcgacc
      901 gtcggcccag gcggtgtgtc ggcccggccg ccgtagggac cggtcgacgc gatgcccagc
      961 gcggtcgaga cgagcccgtc gatgccgctc gctccacggt tcgccatgac ccggaggccg
     1021 tcccggggtg ccatcgccag gtcgaggtcg cggatcggcg tcgagttgcc cacgaaaagg
     1081 gtccccggtt gcgggatcca cgcggcaaga tcacgcgcga tccgaggttc gaacggctcg
     1141 tcgatgccgt cgaggaaccg atccaagcct gatcgcgcga tggtgtcggt ttcggcccac
     1201 cccgatgacc agtcgtcggg cgccgggcgc caacgtcgac gttcgagcgc ctgttgaagg
     1261 gcgtcggcgt ccacggcgag tcgccacgaa gcgtggcgtt ccgggtcgga gtcggggtgc
     1321 cagcgatccg caacgatcaa gcgttctgcg gcgcccacga aggcctgtgt cgcgcgcgag
     1381 gtgggtgtcg cgccgacctg gatcacgacg tcggggcggt gcgtttcgac ccaggctgaa
     1441 ctgcccagga gtgcctggcc ggcggcgagg gactcctcgg gacgtcgcgc tccgctgccc
     1501 ggctcggcga tcaccggcca gccgagcgtc tgtgaccagt acgaggcctc gccgtccagt
     1561 gcgctcggcc accctccgat caccaccgca ccgcgcgcgc cggacaccat gctcgcgagc
     1621 cgatccgctt cctcctcggt gagtgccgct tcggggcgcc tcggcagttc gaggtgctcg
     1681 tcgtccggcg gtggcagcgt ggtgtcctgc gaaggcgtga gcggctcgtc gaagggacag
     1741 ttgacgtgga ccgggccgac ggggtcggag cgcgtggctt ccagggcttg cagcgctgac
     1801 tgggcccacc acgactcttg gcccggggtc tcgggcaccg gcaggtcgag gcactcgcgg
     1861 acgtagcccc cgtacaaccc gacctgatcg atcgtctggt tggcacccgt gccgcgcagg
     1921 cgaggaggcc ggtcggcggt gagcgcgagg agggggatcc gcgaacgcga cgcttccacg
     1981 atggcgggaa ggaactccgc cgccgcggtg ccgctggtgc acgcgatgat caccggccgc
     2041 ctggaggcgt tcgcgaggcc gaccgcgaag aaccctgacg atcgctcatc gaggtggacg
     2101 tggagtcgga tcgaggggtg ccgggcgagg gccaacgcga tcggtgtgga tcgtgaaccg
     2161 ggagacaggc aggcgtcacg cacgcccccg cgcaccgatc cgtcgacgag cgcccaggca
     2221 cacgccagcg agaggtcgcc gaggttcatg tcctcagcca ccggtgagcc tttcgaccga
     2281 tgacagcacc ggcatcccgg tcgccaggac caggcccaag gccgtgagca gaccgcaggc
     2341 cgcgtgggtc gccgcggtgc cggtgaggac cg
//
LOCUS       DATDBZ010000330         3048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2566719, whole genome shotgun sequence.
ACCESSION   DATDBZ010000330 DATDBZ010000000
VERSION     DATDBZ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2566719"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VIX62_07990"
     CDS             <1..369
                     /locus_tag="VIX62_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY5860209.1"
                     /translation="GRVLLDGRDITIRIIGIDTPEKDGPYTDEECFGREASDYTSRVL
                     DGARVGVEFDVDRTDRYERTLAHVWVGAELFGERILRDGLAVLLTIPPNVRYVDRYTA
                     AQATARDDGVGLWGSCASGA"
     gene            complement(383..1960)
                     /locus_tag="VIX62_07995"
     CDS             complement(383..1960)
                     /locus_tag="VIX62_07995"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY5860210.1"
                     /translation="MNDLIVEGATIIDGSGDDGFMGSVGIRGDRIAWIARGETPASVE
                     AAVRLDAQDLVVTPGFVDVHNHSDLCPLVDPDMVSTIRQGVTTVVVGNCGSSPWPVAG
                     AADCAAMAGGSPDAMDLRFASFGDYLDRIERAGPAVNIAALVGHGAVRLDAMGLERRP
                     PTDDELTHMRRSVADAIQQGAVGLSTGLIYVPGLFSQTDEVVALAHAAAQGGGLYASH
                     IRGEGAHLFRAVDEAIEIGRRAGLPAHVSHLKCESSVVWGRAEELLERFHGDADVTTD
                     QYPYTAWSSVLWSLLPEWAPVADVGTLRADPGTHEQLVRAVEHGDETFQSSVNGVGWD
                     RIVIEACGRGRWNGLSVQAIAEARSIQPVEACFDLLEEDPETSCIGHAMDEGDVRTIM
                     ADLEVMVASDAIAMSPQGPLGSLPVHPRAYGTFPRVLGPYVRDGLLGLEAAVRKMTSL
                     PADRFGLRDRGHIAEGGYADLVVLDPSTVQDIADFGDPHAFPEGIGTVIVNGTVAWDA
                     AHPTDPIGRSGRVLRSR"
     gene            complement(1987..>3048)
                     /locus_tag="VIX62_08000"
     CDS             complement(1987..>3048)
                     /locus_tag="VIX62_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5860211.1"
                     /translation="DTASMFTFLGGRVHDLLGPSAATWTTDGRRWGDHIAAADRQASV
                     DAMNEVAEAGTDGSIEYRVVTPDGTTVWIRDVVHVVRGAQGGHQLRGLMVDVTERKRA
                     EQALRKSERKYSEAFRREREAAQRLRALDEMKNTFLEAVSHDLRTPLTSILGSALTLE
                     QTRFELPREESLDLVHRVATNARKLERLLTDLLDLDRLQRGIVSPQRRPTQISALIRR
                     TVDELEDPAGHTIEIDVDDVTVSIDAAKVERIVENLLSNAVRHTPEGTRIWIAASARD
                     GGMMLVVEDEGPGVPTDLREEVFEPFRQAPGSSSEHSPGVGVGLSLVRRFSELHGGRA
                     WLEERPGGGASFHVFLPGG"
BASE COUNT          498 a         1035 c         1021 g          494 t
ORIGIN      
        1 ggccgcgtgc tgctcgacgg gcgcgacatc accatcagga tcatcgggat cgatacgccg
       61 gagaaggatg gcccctacac cgacgaggag tgcttcgggc gcgaagcctc ggactacacg
      121 tcccgcgtcc tggacggtgc ccgcgtcggt gtggagttcg acgtcgatcg cacggatcgc
      181 tacgagcgaa ccctggccca cgtctgggtc ggggcagagc tgttcggcga gcggatcctg
      241 cgggatggcc tcgccgtctt gctgacgatc ccaccgaacg tgcggtacgt ggatcgatac
      301 accgcagctc aggccactgc tcgagacgac ggcgtcggac tttgggggtc ctgtgcgagc
      361 ggggcttgac ccgaacgccc cctcagcgcg aacggagcac ccgtcctgaa cggcctatgg
      421 ggtcggtggg gtgtgctgcg tcccacgcaa cggtgccgtt gacgatcacc gtcccgatcc
      481 cttcagggaa cgcgtgcggg tccccgaagt cggcgatgtc ctgcaccgtc gaagggtcga
      541 gcaccaccag gtcggcgtag ccaccctcgg cgatgtgtcc acgatcgcgc agcccgaaac
      601 ggtcggcagg aagcgaggtc atcttgcgaa cggccgcttc gagccccagc aacccgtcgc
      661 gcacgtacgg cccgaggacg cgcgggaagg tgccgtaggc gcgtggatgc accggcaggc
      721 tgcccaatgg accctgaggg gacatcgcga tcgcatccga tgccaccatg acctcgaggt
      781 cggccatgat cgtgcgcacg tcaccttcat ccatcgcgtg cccgatgcag ctggtctcgg
      841 gatcttcctc gagcagatcg aagcaggcct cgaccggctg gatcgatcgg gcttcggcga
      901 tcgcctggac gctgaggccg ttccatcgtc cgcgaccgca ggcttcgatc acgatccggt
      961 cccacccgac tccgttcacc gacgattgga acgtttcgtc gccgtgttcc acggccctca
     1021 cgagctgttc gtgggtaccg gggtcggctc ggagggtgcc gacatcagcg accggtgccc
     1081 actccggcag gagggaccac agcacggacg accaggcggt gtaggggtat tggtcggtcg
     1141 tcacgtccgc gtcgccgtgg aagcgctcca gcagttcctc ggccctgccc cacacgacgg
     1201 atgattcgca tttcagatgg ctcacatggg cggggaggcc agcgcgacgc ccgatctcga
     1261 tggcttcgtc cacggcccgg aacaggtggg ccccttcacc gcggatgtgc gaggcgtaga
     1321 ggccaccgcc ctgggctgca gcgtgtgcga gggccacgac ctcgtcggtc tgggagaaca
     1381 gaccgggcac gtagatcaag cccgtggaga gccccacggc gccctgctgg atcgcgtcgg
     1441 caaccgaccg acgcatgtgg gtcagttcat catcggtggg aggacgtcgt tccaggccca
     1501 tcgcgtcgag gcgtacggca ccgtgaccga cgagggcggc gatgttgacc gccgggccgg
     1561 ctcgttcgat ccggtccagg tagtcaccga acgacgcgaa ccgcaggtcc atcgcgtcgg
     1621 gcgatccccc cgccatcgcc gcacaatcgg cggcacccgc gacgggccac ggcgacgagc
     1681 cgcaattgcc cacgacgacc gtcgtgacgc cctggcggat ggtcgacacc atgtcgggat
     1741 ccaccaaggg acacaggtcg gagtggttgt gcacatcgac gaacccgggt gtgaccacca
     1801 gatcctgcgc gtccagccga accgccgcct cgacagaggc aggggtctcg ccgcgtgcga
     1861 tccaggcgat gcggtcgccc cggatcccga cggaacccat gaagccgtcg tcgccactcc
     1921 catcgatgat ggtggcgcct tcgacgatga ggtcgttcac gagcgcgatg gtaaacgcag
     1981 gaccattcag ccgccgggca ggaagacgtg gaacgatgcc ccgccccccg ggcgttcctc
     2041 gagccatgca cgtcctccgt ggagctcgga gaaccgtcgg accagggaca gaccgacccc
     2101 cacgccgggt gagtgttcgg aggaggagcc gggcgcttgt cggaagggct cgaagacctc
     2161 ttctcgaagg tcggtcggca cgccaggtcc ttcgtcttcg accaccagca tcatgccgcc
     2221 atctctggcc gaggctgcga tccagatgcg ggtgccttcc ggcgtgtgcc ggaccgcatt
     2281 ggagagcagg ttctccacga tgcgctccac cttcgctgcg tcgatcgaca cggtgacgtc
     2341 gtcaacgtcg atctcgatcg tatggcctgc cgggtcctcg agctcgtcga ccgtcctgcg
     2401 gatcaatgcc gagatctggg tgggccgacg ctgcggcgac acgatgccac gttgcagccg
     2461 atccagatcc agcagatcgg tgagcagccg ctccagtttc cgggcgttgg tagcgacccg
     2521 atggaccaga tccagcgact cctcacgagg gagttcgaac cgtgtctgtt ccagcgtcag
     2581 cgcggagccc aggatcgagg tgaggggcgt ccggaggtcg tgcgagaccg cttccaggaa
     2641 ggtgttcttc atctcgtcga gcgctcggag ccgctgcgcg gcctctcgct cgcggcggaa
     2701 cgcctccgag tatttccgtt cgctcttgcg cagcgcctgt tcggctcgct tacgctccgt
     2761 gacgtccacc atgagtcctc ggagctggtg gcccccctgg gcgccccgca cgacgtgcac
     2821 cacgtcgcgg atccagacgg tcgtgccatc gggtgtgacg acgcggtact cgatcgagcc
     2881 gtccgtgcct gcctcggcga cctcgttcat cgcgtccacc gaggcctggc ggtcggccgc
     2941 ggcgatgtga tccccccaac gacgaccgtc cgtcgtccac gtcgcggccg acggtcccaa
     3001 cagatcgtgc acgcgcccgc ccaggaacgt gaacatggag gcggtgtc
//
LOCUS       DATDBZ010000331         2349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1332619, whole genome shotgun sequence.
ACCESSION   DATDBZ010000331 DATDBZ010000000
VERSION     DATDBZ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332619"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VIX62_08005"
     CDS             <1..1115
                     /locus_tag="VIX62_08005"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY5860212.1"
                     /translation="AICPGEGACGGAYTANTMASVAEALGMSLPGSASPASADRRRDY
                     YAHRSGEAVVNLLRLGITARDILTKKAFENAIAVAMAFGGSTNVVLHLLAIAREADVE
                     LTLDDFNRIGDKVPHIGDLKPFGKYVMNDVDRHGGVPVVMRALLEAGLLHGDALTVTG
                     KTVAENLAEIDPPDLDGKVLRTLDNPIHKSGGITVLKGTIAPEGAVVKTAGFDAQVFE
                     GPARVFERERAAMDALTEGRIEKGDVVVIRYEGPKGGPGMREMLSITAAIKGAGLGAD
                     VLLLTDGRFSGGTTGLCIGHIAPEAVDAGPIAFVRDGDLIRVDIAARTLDLLVDPDEL
                     ATRRTGWAPLPPRYTRGVLAKYTKLVQSAAFGAVTG"
     gene            1159..>2349
                     /locus_tag="VIX62_08010"
     CDS             1159..>2349
                     /locus_tag="VIX62_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5860213.1"
                     /translation="MTTEPSPIPSPAAPATAGKDTTVPEILTGSGAIIRTLELLGVTD
                     VFGIPGGAVIPLYDELMTQDVIRHILVRHEQGAGHAAEGYAAATGRVGVAIATSGPGA
                     TNLVTAIADAYMDSVPILCITGQVFSTSMGTDAFQEADIVGITMPITKHSFLVTRPEE
                     VPAAIAAAYHIASTGRPGPVLVDITKDAQQKSAPFIWPPKLDLPGYRPITKAHGKQVQ
                     AAAQLFAEAQKPVLYVGGGVIRAHASAELLKLAELTGAPVVTTLMARGAFPDSHELHV
                     GMPGMHCTVPAVLALQESDLIVSLGARFDDRVTGKVSEFAPNAKIVHVDVDPAEISKI
                     RSADVPIVGDAKDVIADLTVAYEAATADSAPDLSAWWSYLNGLRVEYPLGFSEPSDGL
                     LAPQY"
BASE COUNT          384 a          867 c          739 g          359 t
ORIGIN      
        1 gcgcgatctg tccgggcgag ggcgcctgcg gtggggcgta caccgcgaac acgatggcga
       61 gcgtcgcgga ggcgctcggg atgagcctgc ccggctccgc atcgccggca tcggccgacc
      121 gccgccgcga ctactacgcg caccgctccg gcgaggccgt cgtcaacctg ctgcgcctcg
      181 ggatcaccgc ccgcgacatc ctcaccaaga aggcgttcga gaacgcgatc gccgtcgcga
      241 tggcgttcgg cggttccacc aacgtggtgc tgcacctcct ggcgatcgcg cgtgaggccg
      301 acgtcgagct caccctcgat gacttcaacc gcatcggcga caaggttccg cacatcggcg
      361 acctcaagcc cttcggcaag tacgtgatga acgacgtcga ccggcacggt ggtgttcccg
      421 tggtgatgcg ggccctgctc gaggccggcc tgctgcacgg cgacgcgctc accgtcaccg
      481 gcaagaccgt ggccgagaac ctcgccgaga tcgacccgcc cgacctcgac ggcaaggtgc
      541 tgcgcaccct ggacaacccg atccacaaga gcggcgggat caccgtgctc aagggcacga
      601 tcgcccccga gggagccgtc gtcaagaccg cgggtttcga cgcgcaggtc ttcgagggcc
      661 cggcgcgggt gtttgaacgc gaacgcgccg cgatggacgc cctgaccgag ggacgcatcg
      721 aaaagggcga cgtcgtcgtc atccgttacg agggcccgaa gggtggcccc ggcatgcgcg
      781 aaatgctctc gatcaccgcg gccatcaagg gcgccgggct cggggccgat gtactcctat
      841 tgactgatgg tcggttctca ggcggcacaa ccggactgtg catcggccat attgcacccg
      901 aagcggtgga cgcaggtccc attgccttcg tgcgcgatgg tgatctgata cgggtcgata
      961 tcgcagctcg cacgcttgac ctactggtcg accctgatga gctcgccacc cgccgcaccg
     1021 gttgggcccc ccttcccccg cgatacacgc ggggcgtcct ggccaagtac acgaaactcg
     1081 tgcaatccgc ggctttcggt gcggtaaccg gctaggcctt ctgctcaccg tccgtaattc
     1141 aagtagggac tgccttccat gaccacggaa ccttcgccca tcccatcgcc cgcagctccc
     1201 gcgaccgcgg gtaaggacac gaccgtaccg gagatcctca cgggctccgg tgcgatcatc
     1261 cgcaccctcg agctcctcgg ggtcaccgac gtcttcggca ttcccggcgg cgccgtcatt
     1321 ccgctttacg acgaactgat gacccaggat gtgatccgcc acatcctggt gcgacacgag
     1381 cagggtgccg gccacgcggc cgaggggtac gccgcggcga ccggacgcgt gggcgtggcc
     1441 atcgccacct ccgggcccgg cgcgaccaac ctcgtcacgg ccatcgcgga tgcctacatg
     1501 gactccgtgc ccatcctctg catcaccggc caggtgttct cgacctcgat gggcaccgac
     1561 gcgttccagg aggccgacat cgtcggcatc acgatgccga tcaccaagca ctccttcctc
     1621 gtcacccgcc cggaagaggt gccggcggcc atcgccgcgg cgtatcacat cgcctccacc
     1681 ggccgaccgg gcccggtgct cgtcgacatc accaaggacg cgcagcagaa gtcggcaccg
     1741 ttcatctggc cgcccaagct cgacctcccc ggctaccgcc cgatcaccaa ggcccacggc
     1801 aagcaggtgc aggccgcggc gcagctcttc gccgaggcgc agaagcccgt gctctacgtc
     1861 ggcggcggcg tcatccgcgc ccatgcgtcg gccgagctgc tgaaactcgc cgaactgacc
     1921 ggcgccccgg tggtcaccac gctgatggcc cgaggcgcct tcccggactc gcacgaactg
     1981 cacgtcggga tgcccgggat gcactgcacc gttccggcgg tgctcgcgct tcaggagagc
     2041 gacctgatcg tgtcgctcgg cgcccggttc gacgaccgcg tcaccggcaa ggtgtccgag
     2101 ttcgccccga acgcgaagat cgtgcacgtc gacgtcgacc ccgcggagat ctcgaagatc
     2161 cgcagcgcgg atgtaccgat cgtcggcgat gccaaggacg tcatcgccga cctcacggtc
     2221 gcctacgagg ccgcgacggc ggattccgct cccgacctca gcgcctggtg gtcgtacctc
     2281 aacggcctcc gcgtcgagta cccgctgggc ttctccgagc cctccgacgg cctcctcgct
     2341 ccccagtac
//
LOCUS       DATDBZ010000332         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_91992,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000332 DATDBZ010000000
VERSION     DATDBZ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91992"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VIX62_08015"
     CDS             <1..356
                     /locus_tag="VIX62_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860214.1"
                     /translation="RTVTAPVVPPDRVGIAGWGAPQAPAIIGDACYVHVAAEAGAAAG
                     IAERLGDMSDVSGVAVLGGQYDLMVCVAQPWEVASGVILEEIHPLPGVVSTDTLISIN
                     YEEPDEDRDQFSAWT"
     gene            complement(363..1334)
                     /locus_tag="VIX62_08020"
     CDS             complement(363..1334)
                     /locus_tag="VIX62_08020"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY5860215.1"
                     /translation="MDSANAAPTGGTVLLVAPRGYCAGVERAVDAVERALAKHGSPVY
                     VRKQIVHNLHVVRDLESKGAIFVDEETEVPAGAVVVLSAHGVAPQVYANSAARDLEVI
                     DATCPLVTKVHVEARRFAKDGRTIVLIGHAGHEEVEGTSGQAPERTILVQSPAEARTV
                     EVPDPENLRYLTQTTLSVDETNEIVRILTERFPSIEGPPRDDICYATQNRQDAVKVIA
                     PRSDVLLVIGSRNSSNSNRLAELSKELGTPAHLVDDEGDVDDAWLDGASTVGLTSGAS
                     APEWLVHRMLAWLAERGFAQVEEVTLTEERVRFSLPAGVRQVPLIAT"
     gene            1713..2159
                     /locus_tag="VIX62_08025"
     CDS             1713..2159
                     /locus_tag="VIX62_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860216.1"
                     /translation="MTFRTLRRRRFPAALALTAVLLIVPAASHAGAATPGAGYSEVPR
                     KICPIDWRDGTFHVKKLIRCAAARWTVPGGAAKALAIANRESNFHPRAYNSYSGAAGI
                     YQHLRHYWPGRARVYGFRGWSAFNARANIMVTMRMVHRGGWGPWGG"
BASE COUNT          322 a          753 c          756 g          352 t
ORIGIN      
        1 gacgcacggt caccgcgccg gtcgtgccgc cggaccgtgt cggcatcgcc gggtggggcg
       61 ccccacaggc cccggcgatc atcggcgatg cctgctacgt gcatgtcgcg gccgaagcgg
      121 gggcggcggc cggcatcgcc gagcgcctcg gcgacatgag cgacgtctcc ggggtcgcgg
      181 tgttgggggg tcagtacgac ctgatggtct gcgtggccca accctgggag gtcgcgagcg
      241 gcgtgatcct cgaggagatc catccgttgc ccggggtcgt gtcgaccgac accctcatct
      301 cgatcaacta cgaggaaccc gacgaggacc gcgaccagtt ctcggcatgg acctgagcat
      361 cctcaggtgg cgatcagcgg cacctgacgc acgcccgcgg gcaaggagaa ccgcacgcgc
      421 tcctcggtga gcgtgacctc ctcgacctgg gcgaaccccc gctcggcgag ccacgcgagc
      481 atgcggtgca cgagccactc gggcgccgag gcaccggagg tcaggccgac ggtgctcgcg
      541 ccatcgagcc aggcgtcatc gacgtccccc tcgtcgtcga ccaggtgggc gggcgtgccg
      601 agctccttcg agagttcggc gaggcggttg gagttcgatg agttgcgcga accgatcacg
      661 agcagcacgt cggagcgcgg ggcgatgacc ttcacggcgt cctgccggtt ctgggtggcg
      721 tagcagatgt catcgcgcgg cgggccctcg atcgaaggga agcgctccgt gaggatgcgc
      781 acgatctcgt tcgtctcgtc gaccgacagc gtggtctggg tgaggtagcg caggttctcg
      841 ggatcgggga cctcgacggt gcgggcctcg gcgggcgact gcaccaagat cgtgcgctcg
      901 ggcgcctggc cgctcgtgcc ctcgacctct tcatgtccgg catggccgat cagcacgatc
      961 gtgcggccgt ccttggcgaa cctgcgggcc tcgacgtgca ccttcgtgac gagcgggcag
     1021 gtggcgtcga tcacctcgag atcgcgtgcc gccgaattgg cgtagacctg cggtgcgacc
     1081 ccgtgtgcgg acagcaccac cacggcgccc gcgggcacct cggtctcttc atcgacgaag
     1141 atcgcgccct tggactcgag gtcgcgcacg acgtgcaggt tgtggacgat ctgcttgcgg
     1201 acgtagaccg gcgagccgtg cttggccagc gcacgctcga cggcgtcgac cgcgcgctcc
     1261 acaccggcgc aatagcctcg aggggccacc agcagcacgg tgccccctgt gggggcggcg
     1321 ttcgcggagt ccatggctcc atggtagccg ggacctgtgt tgatccaccg gcacatgact
     1381 tcgggtcacg gttcggttgt cgtatgtcgg tgggctcccc tagggtggtc gggcacgaca
     1441 cggaccgttc ggtccgctcc tccccggcgc gacgatgcgc aacctccttc ccaaggttgg
     1501 tgtgcgtatg cctgcttgcg cccgggagcc ggggctcgga cgaccgcggt cgtgcgatag
     1561 gtcacacaga gatggatacc gacccacagg aggtgatgtc cgcacccacg acctaccgcg
     1621 gggacgtcgg cgcagcggtg aagagttcac cgcacagccg gacgacctga gcgcagcggc
     1681 cagttccgct cactgcgggg gtgactcgat ctatgacgtt ccgtaccctt cggcgccgcc
     1741 gcttcccggc cgcgctggcc ctaaccgccg tgctcctcat cgtccccgcc gcatcacatg
     1801 cgggcgctgc tacacccgga gccggctact ccgaggttcc gcgcaagatc tgcccgatcg
     1861 actggcgcga cggaaccttc cacgtcaaga agctcatccg ttgcgccgct gcgcgttgga
     1921 ccgttccggg tggggccgcc aaggcccttg ccatcgccaa ccgcgagtcc aacttccacc
     1981 cccgtgccta caacagctac agcggggccg ccggcatcta tcagcacctc cgtcactact
     2041 ggcctggccg ggcccgtgtg tacggattcc ggggctggtc ggcgttcaat gcgcgcgcca
     2101 acatcatggt gaccatgcgc atggtgcacc gaggtggttg gggcccctgg gggggctaac
     2161 ccccactgac cggaccgccc ggg
//
LOCUS       DATDBZ010000333         5211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2775734, whole genome shotgun sequence.
ACCESSION   DATDBZ010000333 DATDBZ010000000
VERSION     DATDBZ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2775734"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..710)
                     /locus_tag="VIX62_08030"
     CDS             complement(6..710)
                     /locus_tag="VIX62_08030"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019072554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HEY5860217.1"
                     /translation="MTERHIVAMGGGQWPSDPIYPFLFELADAAKPKVLYVPTATGDN
                     DRGVAMFYRSFPAHSFEPSDLGLFARTVEDLRSFVLAQDVVLVAGGNTLNMLAIWRAH
                     GLDVVLRDAWEAGVIMAGGSAGANCWFEASTTDSYLMGNADPLPDGLGLVPGSFCPHY
                     DSEPSRQPAYRALVAAGTLPPGIACDDLAAAHIVGADLAEIVVSADGSGARRVEPDDR
                     DGASETVLPARLLEGP"
     gene            complement(707..2365)
                     /gene="hutU"
                     /locus_tag="VIX62_08035"
     CDS             complement(707..2365)
                     /gene="hutU"
                     /locus_tag="VIX62_08035"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY5860218.1"
                     /translation="MSHRNIRAPRGTDLSCRSWPQEAAMRMLMNNLDPEVAERPEDLV
                     VYGGTGRAARSWEAYDAIVRTLRTLGDDETLLVQSGKPVAVFRTHDLAPRVLISNSML
                     VPKWADWETFRALEAQGLTMYGQMTAGSWIYIGTQGILQGTYETLAECAQQHFGDSLA
                     GTITLTGGLGGMGGAQPLAVTMNGGVALCVDVDPGRIERRIQTRYLDRMTADLDEAVD
                     WAEAAKASREAVSIGVVGNTAEVFPALLGRGFAADVVTDQTSAHDPLGGYVPEGLSLP
                     EADALRREPDEYVQRSYESMAHQVDAMVDFAEAGSVVFDYGNNLRAGAVEGGLDRDRA
                     YSFPGFVPAFVRPLFCEGKGPFRWAALSGDPADIAATDRAVAELFPDNDRLHRWLRMA
                     AERVAFQGLPARICWLGYGERDRAGERFNEMVASGEVSAPIVIGRDHLDAGSVASPNR
                     ETEAMLDGSDAIADWPILNALINTAAGAHWVSVHHGGGVGIGYSIHAGMVVVADGTAN
                     AGERLARVLRSDPGMGVVRHVDAGYDRAIDVAHERGVRVPMEES"
     gene            2489..3694
                     /locus_tag="VIX62_08040"
     CDS             2489..3694
                     /locus_tag="VIX62_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5860219.1"
                     /translation="MAVGTSPYAPGGASSMGADPIRVLIADDEPALRIALADLLAHDD
                     EVVLIGSAGDADEAIQLAVDEHPDVALVDVKMPAGGGPRAAREIMRVSPDTRVIALSA
                     FEDRPTVLEMLRAGAVGYLVKGTAGEEILGSIQKVMAGGASLSSEVIAGIVHELTQQL
                     RREEAAREERDARRGEIERFVAGEGLSMVFQPIVELSSRSTVGVEALARFRSLPLRPP
                     NEWFAEAVQLALGVQLELTTIAQALRELDRVPEGAYLAVNCSHHAATSSQLARLLGSD
                     AGRIVLEITEHEAVEDYVALVTALAPLREMGARIAIDDAGAGFASMRHTLQITPDIVK
                     MDMSLTRGIDTDRAKRALAAALISFTEEMDIVMVAEGIETVEELDTLATLGVRFGQGF
                     YLAEPAQLA"
     gene            complement(3767..4720)
                     /locus_tag="VIX62_08045"
     CDS             complement(3767..4720)
                     /locus_tag="VIX62_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5860220.1"
                     /translation="MDPTADAGGGESLLVVDDDPFIARLLEIELRAAGYDVRVASDGS
                     LALNAAQERCPDLVLADVMMPNMDGFELTRRLRQDPRTASVSIIMLTARGLSADKLEG
                     FAIGADDYIVKPFDTPELLARIRGVLRRSRDMRAQSPLTGLPGNVRIEEEIDGRVDQG
                     IPFAILYADLDHFKAFNDHYGFMRGDQVIQSSARLFEEVSSDETGGAAFVGHVGGDDF
                     VVVVDPGKAEAVAQAIVERFDREAPGLYDPDDRDRGYIEVTNRRGELQRFPMLTVSIG
                     VASTDKRAFQHYAEAVAVATEMKQFTKGSTGSSWAMDRRVG"
     gene            4970..>5211
                     /locus_tag="VIX62_08050"
     CDS             4970..>5211
                     /locus_tag="VIX62_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019066696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5860221.1"
                     /translation="MQVLDTFLDAMGDTPLVRLHSVTRGVEATVLAKVEMLNPGGSVK
                     DRIGIRMIEAAERAGTLKPGGTIVEPTSGNTGHGLA"
BASE COUNT          783 a         1846 c         1786 g          796 t
ORIGIN      
        1 gacggtcatg gcccctccag gagccgggcc ggcaacacgg tctctgatgc gccgtcgcga
       61 tcgtcgggct cgacgcgtct ggcgcccgac ccgtcggccg agaccacgat ctcggcgaga
      121 tccgcgccca cgatgtgggc ggccgcgagg tcgtcgcacg cgatgcccgg tggcaaggtg
      181 ccggcggcga ccagcgcgcg gtaggccggc tggcgcgagg gctcgctgtc gtagtgcggg
      241 cagaagctcc ccggcaccag gcccaggccg tcgggcagcg gatcggcgtt gcccatcagg
      301 taggagtcgg tggtgctggc ctcgaaccag cagttggccc cggcgctgcc gcccgccatg
      361 atcacgcccg cctcccacgc gtcccggagc accacatcga ggccgtgcgc gcgccagatg
      421 gccaacatgt tcagcgtgtt gccgcctgcc acgaggacca cgtcctgggc caagacgaac
      481 gaacgcaggt cctcgaccgt tcgcgcgaac agcccgagat cgctcggctc gaacgagtgc
      541 gcggggaagg agcggtagaa catcgccacg ccgcgatcgt tgtcaccggt cgccgtgggc
      601 acgtagagca ccttgggttt ggccgcatcg gccagctcga acaggaacgg atagatcgga
      661 tccgagggcc actgccctcc acccatcgcc acgatgtggc gttcggtcat gactcctcca
      721 tggggacacg gacgcctcgt tcgtgcgcga cgtcgatcgc gcggtcgtac ccggcgtcga
      781 cgtgccgcac cacgcccatg cccggatcgg atcgcagcac ccgcgcgagc cgctcaccgg
      841 cgttcgcggt gccgtcggcc accaccacca tgcccgcgtg gatcgagtat ccgatcccca
      901 cgccgccccc gtggtgcacg ctcacccagt gcgcgcccgc ggccgtgttg atcaacgcgt
      961 tgaggatcgg ccagtcggcg atcgcgtccg acccgtccag catcgcctcg gtctcgcggt
     1021 tgggactggc gacgctgccg gcgtccaggt gatcgcgccc gatcacgatc ggggccgaca
     1081 cctcgcctga ggccaccatc tcgttgaagc gctccccggc gcgatcgcgc tcgccgtagc
     1141 cgagccaaca gatccgtgcg ggcaacccct ggaaggcgac acgctcggcg gccatccgca
     1201 gccaacggtg caggcggtcg ttgtccggga agagttcggc caccgcccgg tcggtcgcgg
     1261 cgatgtcggc cggatcgccg ctgagcgctg cccagcggaa cggtcccttg ccttcgcaga
     1321 agagcgggcg cacgaacgcc ggcacgaacc ccgggaacga atacgcccgg tcgcggtcga
     1381 gtccgccttc caccgccccc gcccgcaggt tgttgccgta gtcgaagacc acggagccgg
     1441 cctcggcgaa gtccaccatg gcgtccacct ggtgggccat cgactcgtag gagcgctgga
     1501 cgtactcatc gggctcgcgc cggagcgcat cggcctcggg cagcgacaac ccctcgggca
     1561 cgtacccgcc gagcgggtcg tgtgcgctgg tctggtcggt gaccacgtca gcggcgaacc
     1621 cgcgcccgag caacgcgggg aagacctcgg cggtgttgcc gaccaccccg atcgagaccg
     1681 cttcgcgcga ggccttcgcc gcttcggccc agtccacggc ctcgtccaga tcggcggtca
     1741 tgcggtcgag gtagcgcgtc tggatccgtc gctcgatccg cccggggtcg acgtcgacgc
     1801 agagcgccac gccgccgttc atcgtcaccg ccagcggctg ggccccgccc atgccgccga
     1861 gcccgcccgt caacgtgatc gtgccggcga gcgaatcacc gaagtgctgc tgcgcgcact
     1921 cggcgagggt ttcgtacgtg ccctgcagga tgccctgggt cccgatgtag atccacgagc
     1981 ccgcggtcat ctgcccgtac atcgtgagcc cctgggcctc caaggcacgg aacgtctccc
     2041 agtcggccca tttcgggacc agcatggagt tggagatcag gaccctcggt gcgagatcgt
     2101 gcgtgcggaa caccgcgacc ggcttgcccg actgcaccag cagggtctcg tcgtcgccca
     2161 gtgtcctcag ggtgcgaacg atggcgtcgt aggcctccca gcttcgggcg gcgcgtccgg
     2221 tgccgccgta gacgaccagg tcctcgggac gctcggccac ttcgggatcg aggttgttca
     2281 tgagcatccg catcgccgct tcctgcggcc acgaacggca cgacagatcg gtgcctcgcg
     2341 gtgccctgat gttgcggtgg ctcatcgatc ccctcccccg tccggccgat gctacccgcc
     2401 gggcgcccgg gaggccgttc gagacgagcc tggggagaaa cggctcatgt gcttgagcag
     2461 ggggttgctg ccgggtagcg tccccccgat ggccgtcgga acttctccct acgcgccggg
     2521 tggtgccagc agcatgggcg ccgacccgat ccgtgtcctc atcgctgacg acgaacccgc
     2581 cctgcgcatc gccctcgccg acctcctggc ccacgacgac gaggtggtgc tgatcgggag
     2641 cgccggcgac gccgacgagg cgatccaact cgccgtggac gagcaccccg acgtggccct
     2701 ggtcgacgtg aagatgccgg ccggcggtgg accccgagcg gcacgggaga tcatgcgggt
     2761 atcccccgac acccgggtca tcgcgctctc cgccttcgag gaccgtccca cggtcctcga
     2821 gatgctgcgc gccggtgcgg tcggctacct ggtgaagggc accgccggtg aggagatcct
     2881 cggttcgatc cagaaggtca tggccggcgg cgcgagcctg tcatcggagg tgatcgccgg
     2941 catcgtgcac gagctcaccc agcagctccg acgcgaggag gcggctcgcg aggagcgcga
     3001 tgcccgccgc ggcgagatcg agcggttcgt ggcgggcgag gggctgtcca tggtgttcca
     3061 gcccatcgtc gagctctcga gccggagcac cgtcggggtc gaggcgctgg cccggttccg
     3121 ttcgctcccg ttgcgaccac cgaacgaatg gttcgccgag gcggtccagc tggcgttggg
     3181 cgtgcagctg gagctcacca cgatcgccca ggcactgcgc gagctggacc gggtgcccga
     3241 gggcgcctac ctggccgtga actgctcgca tcacgcggcc acctcctccc aactggcgcg
     3301 tctactggga tcggacgccg gccggatcgt gctggagatc accgaacacg aggcggtcga
     3361 ggactacgtc gccctggtga cggcgctcgc ccccctgcgc gagatgggcg cccgcatcgc
     3421 gatcgacgat gccggcgcgg gattcgccag catgcgccac accctgcaga tcacccccga
     3481 catcgtgaag atggacatga gcctcacccg ggggatcgac accgaccgcg cgaaacgggc
     3541 cctcgcggcg gccctgatct cgttcaccga ggagatggac atcgtcatgg tggccgaagg
     3601 catcgaaacg gtggaggaac tcgacacgct cgcgaccttg ggcgttcgct tcggtcaggg
     3661 cttctacctg gccgaacccg ctcagctggc ctagcgtcgc cgcctgctag ccggtgcgtc
     3721 ggtccatcgc gcgttcctag ccgacgcggc ggtccatcgc gtcttcctag ccgacgcgtc
     3781 gatccatcgc ccaggacgag ccggtggaac ccttcgtgaa ctgcttcatc tcggtggcca
     3841 cggccaccgc ctcggcgtag tgctggaagg cgcgcttgtc ggtggaggcg accccgatcg
     3901 agaccgtgag catggggaag cgttgcagct cgccgcgacg gttcgtgacc tcgatgtagc
     3961 cccggtcacg gtcgtccggg tcgtacaggc ctggggcctc ccggtcgaac cgctccacga
     4021 tcgcctgggc cacggcctcg gccttgccgg ggtcgaccac gaccacgaag tcgtcgccgc
     4081 cgacatgacc gacgaacgcg gctccaccgg tctcgtcgct cgagacctct tcgaagaggc
     4141 gggcgctcga ctggatcacc tggtcgccgc gcatgaaccc gtagtggtcg ttgaaggcct
     4201 tgaagtgatc gaggtcggcg tacaggatcg cgaacgggat cccctggtcc acgcggccgt
     4261 cgatctcctc ttcgatccgg acgttgccgg gcaggcctgt gagcggcgac tgcgcgcgca
     4321 tgtcgcgtga tcggcggagc acgccgcgga tccgggccag cagctcgggc gtgtcgaagg
     4381 gcttgacgat gtagtcgtcg gcgccgatcg cgaacccttc cagcttgtcg gccgagaggc
     4441 cgcgtgccgt cagcatgatg atcgagaccg acgcggtgcg ggggtcctga cgcaggcgcc
     4501 gggtgagttc gaacccgtcc atgttgggca tcatcacgtc ggcgagcacg aggtcgggac
     4561 agcgctcctg ggcggcattc agcgcgagcg atccatcgga ggccacgcgc acgtcgtagc
     4621 cggcggcccg caactcgatc tccagcaggc gcgcgatgaa cggatcgtcg tcgaccacca
     4681 ggaggctctc gccacccccg gcgtcggcgg tcggatccac gttcaatcct tcggcttcgt
     4741 tcacacccgc acctgtgctc ggtccatctg ctcggtcacg tgtcccccgt gtcccctcgc
     4801 gaatcgcttg ggtcacgaat cggcacttta gtcccgaagg tgaagcctgt ccgggtagcg
     4861 atctgttctt tgccgcacac gccccgaacg cggtggcacg cgggacggac cggtccgggc
     4921 gtggcaccat aggccccgac cgcatgcggc gaagggggct cggtccacga tgcaggtgct
     4981 cgacacgttc ctggacgcga tgggcgacac gccgctcgtg cgcctccatt cggtgacccg
     5041 aggcgtggag gccacggtcc tggccaaggt cgagatgctg aatccgggcg gttccgtcaa
     5101 ggaccggatc gggatccgca tgatcgaggc cgcggagcgt gcgggcaccc tgaagcccgg
     5161 cgggacgatc gtggagccca cgagcggcaa caccgggcac ggtctcgcga t
//
LOCUS       DATDBZ010000334        10554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2568085, whole genome shotgun sequence.
ACCESSION   DATDBZ010000334 DATDBZ010000000
VERSION     DATDBZ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2568085"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VIX62_08055"
     CDS             complement(<1..214)
                     /locus_tag="VIX62_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860222.1"
                     /translation="MSSADEAPVAEIPGDTLRTGLLDRFREVLGDALVDSHLKTGDDL
                     WLRIRSDSWRAAGQAARQLGFDYFCFL"
     gene            complement(201..812)
                     /locus_tag="VIX62_08060"
     CDS             complement(201..812)
                     /locus_tag="VIX62_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY5860223.1"
                     /translation="MGLITEKTSLAKVQPVKFILNWGRRYSLWVMNFGLACCAIEFIA
                     ASTAKNDFIRLGVIPFAHGPRQADLLVVAGTLTDKLAPALKRVYEQMPEPKYVISMGS
                     CANCGGPYWDSYSVTKGVDQIIPVDIYVPGCPPRPEALLEAVVLLQQRIKNEDMGARW
                     RGEGTKLLASAPAKSDAAARTPASESPPAALPSGDGGETVVVG"
     gene            complement(803..1168)
                     /gene="ndhC"
                     /locus_tag="VIX62_08065"
     CDS             complement(803..1168)
                     /gene="ndhC"
                     /locus_tag="VIX62_08065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY5860224.1"
                     /translation="MGDYYSNYGVVLAVLTAGVLLVAVAFGTARLVAPHKPNREKLTT
                     YECGIDPVGEGWSQSQVRYYVYGFLFVIFDVESVFLFPWARVFESLGYAAVVEMAIFI
                     GILAVGLLYAWRKGVLRWA"
     gene            complement(1226..2236)
                     /locus_tag="VIX62_08070"
     CDS             complement(1226..2236)
                     /locus_tag="VIX62_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5860225.1"
                     /translation="MSRRVIPGLEAPDAALEADIRARLDRVEDALKKAVGADSELLAT
                     TAKYLLTAGGKRFRPMLVLLSGYFGDPTDPRLIPGSVAIELVHQATLYHDDVIDEADE
                     RHGVPAANARWDNTVAILTGDYLFARASELSADLGPEVCRLLAQTIAVLCDGQIREVD
                     SSGKVEQDQDNYLGIIRRKTGVLIATSCRLGGMLSDATTEHTEVLEGFGESLGMAFQL
                     SDDIMDFTASQLELGKEPGQDMKEGVYTLPVLHALHEGPHRDELASLLRHGAPDGERL
                     DRALEIVRTGGSIEHARRATAEEVARAVGLARRLPDGHAQSALIQLARFLAVRCGAEA
                     GE"
     gene            complement(2265..3716)
                     /gene="nuoN"
                     /locus_tag="VIX62_08075"
     CDS             complement(2265..3716)
                     /gene="nuoN"
                     /locus_tag="VIX62_08075"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY5860226.1"
                     /translation="MIETPTLVFGPVLPELILVGVGIIGLLYEALVRKPDRSIHLAIG
                     LLGLLAAAIATLRLWDWDGDPVVLGGSVSADKFAVVACTILLVIAAFGLVYGTHYFSR
                     DPAAARGEYYPLVLFATAGMTLIAASADLIVMFLALEILSLSLYVLTGITGARRANEA
                     AMKYFLLGAFASAFFLYGVAMAYGAVDTTNIVQIVRSMSGQTGNQALALLSFAFLAIG
                     FGFKVSAAPFHMWTPDVYQGAPTPVTAYMSAATKVAAFAALIRVLMVAFQPLTWDWQP
                     VIWVLAALSIVVGSVLAIAQTDIKRMLAYSSVAQAGFILTGLVSGTAVGIRSALFYLV
                     AYSAMTLGAFGVVMLVSAKGEEQTSITSYAGLSKRSPLLAGLMAIFMLSLAGIPPTAG
                     FIAKVGVFASAIGAGAWPLAVIGVLSSVVAAFFYLRVIVVMYMQEPQGEYDRDDALTP
                     RLVLVIPAVVTIALGVFPGLIVGILEQASVLKW"
     gene            complement(3713..5275)
                     /locus_tag="VIX62_08080"
     CDS             complement(3713..5275)
                     /locus_tag="VIX62_08080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5860227.1"
                     /translation="MNRLLTIVTFLPLVGLLVIMVGGKELKDDAARWITLVVALATFV
                     VSLVVLGRFEQGDPGFQMVEQATWVKDVGLQYLVGIDGISIWMVLLTTFLFPVAVLAS
                     WKIDKDVRLYMGAMLALETAVLGSFVSLDLLLFFLFFEAILVPMYLLIGGWGGERRVY
                     AAVKFFLYTMAGSAFLLVSIIFLYSRSGAVLGGANTFDLRQLGEVGAAIPLATARWLF
                     LGFFIAFAVKVPVFPLHTWLPDAHTEAPTAGSVLLAGVLLKVGTYGLIRFNLALFPGA
                     SKYFATFVSILAVIGIVYGAVCALIQTDIKRLVAYSSVSHLGFVVLGIFAFTEQAVTG
                     GVLQMVNHGLATGALFLLVGMVYERTHTRDLAKMGGLASVMPWLTGAFLFAVFASVGL
                     PGLNSFVGEFLVIVGTFAVNHWFGSIAAIAVVLAAIYLLWSYQRMAFGPVRDEHRHLP
                     DVSLREVAVLAPVLALLLVFGVYPKILTDRIDPSTEAVIAHVDPQGQTTDVGDVQLRV
                     VPSELSPPEEEG"
     gene            complement(5272..7191)
                     /gene="nuoL"
                     /locus_tag="VIX62_08085"
     CDS             complement(5272..7191)
                     /gene="nuoL"
                     /locus_tag="VIX62_08085"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY5860228.1"
                     /translation="MVAESVGLVPDPNQAMQVLWLVPLLPLVGAVINLFVGKRLGKAA
                     GWLAVVMMALSFAVAVLAVKGLLDAPEGERLFLKHLSDWISVGSLRVNFDLRLDALSA
                     TMILVVTGIGTLIHVYAIGYMDGDGRFGRFFAYLNLFVFFMLMLVLGANLLVLYLGWE
                     GVGLCSYLLIGFWFDKTANANAAKKAFVTTRVGDTLMLVGLAVVVMKFGTLDYEVIFG
                     AAGSTLTKSAATVISLLLFAGAVGKSAQLPLHVWLPDAMAGPTPVSALIHAATMVTAG
                     VYLVVRMHPVFDLSPVALTVVTVTGLVTAIYAGTCALGQDDIKRVLAYSTISQLGFMF
                     LAAGLKAYSAAIFFLVAHAFYKALMFLGAGSVMHGMHEETDLKVMGGLWRRMPYTAIT
                     FTLGALALSGVPPLAGFFAKDAVLEIAQSNSEWGYYLLGTLAAFISALYMGRVIFLAF
                     YGTARSEAAEHAHESPPVMWIPLVLLAVGAVTVGALATHPEGLLATFLEPISGLVPEG
                     SEGLSTPVFYVVALLVTFGGLGTAWFVYGSGRVDWLSLRVRLAPLHRLFANGWYIDEY
                     YAALIVTPGKAIAAFTAYRIDVGFIDGIVNGIGSAFRRLAGVGRNIQTGFVRTYAAAV
                     FVGAVAIVVYVGFRL"
     gene            complement(7185..7490)
                     /gene="nuoK"
                     /locus_tag="VIX62_08090"
     CDS             complement(7185..7490)
                     /gene="nuoK"
                     /locus_tag="VIX62_08090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY5860229.1"
                     /translation="MRTPIAYFVMLSGILFAIGSVGVLIRKSALLIFMSVELQLNAVN
                     LALVAFSRMNGDLRGQVLAFFSMVVAAAEVVVGLAIIVSIYRRRATVNVDDARELRW"
     gene            complement(7487..8116)
                     /locus_tag="VIX62_08095"
     CDS             complement(7487..8116)
                     /locus_tag="VIX62_08095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY5860230.1"
                     /translation="MPLDNVVFWVFAPVSVASAIGMLVMRNAVHAALFLIVNFFTIAV
                     MYLLLDAPFLFAVQIIVYAGAIMVLFLFVIMLLGIDRGDDVHEPLIGQRPLAVILAVG
                     FVLEIGFALRAGIGFATRAPDGFDVVNQGGNAQALATVLFRDYFFPFEVTSVLLIIAA
                     VAAMVLAQRKSRAITQAEIDEHRADLDALGHTNPGAPKPGAPLEAGEPR"
     gene            complement(8123..8626)
                     /gene="nuoI"
                     /locus_tag="VIX62_08100"
     CDS             complement(8123..8626)
                     /gene="nuoI"
                     /locus_tag="VIX62_08100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY5860231.1"
                     /translation="MTDDRDPRERGGVWDSTVAIAKGMGTVFKQTFRPDNTEQYPKEI
                     APIPERSHIGRHRLNKHEDGLEKCIGCELCAYACPADAIWVEGADNDPEAPTSPGERY
                     AKDYQINYLRCIMCGLCVEACPTRALTLTSYFEMGFTSREEAIWTKEQLLTPPPELGT
                     DPVDREG"
     gene            complement(8623..9798)
                     /gene="nuoH"
                     /locus_tag="VIX62_08105"
     CDS             complement(8623..9798)
                     /gene="nuoH"
                     /locus_tag="VIX62_08105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY5860232.1"
                     /translation="MDWVDWSVMVVRVVLVFFVLLIAVLLYIWMERKVIADMQTRVGP
                     MRAGPRGMLVTLADGIKLFFKEGITPTNADRPVYLIAPILAMFPGFLAFCTIPFGEPV
                     ELFGRVVPFQITDLNIGILWVLAMTSIGVYGVVLAGWSSGSNYPLLGSVRSSAQMISY
                     EVGMGLALVAVIMWSGHLSMSEIVAQQSGNFALHWHDATLFGVPAWNIFPQFPAFVVF
                     FICALAETNRPPFDLAEAESELVAGFHTEYSGIKFAMFFLGEYVSTVTIAAVGVTLFL
                     GGWHGPWFDGPLSWIGPLLWFLLKITAVIYVMILVRATLPRMRYDRLMQFGWKVLIPF
                     GLLWVMVTGLVVVIPQEYGNQGKFLMFAVMCGTLALALLLAPLFSRGSARSRKEVGA"
     gene            complement(9798..>10554)
                     /locus_tag="VIX62_08110"
     CDS             complement(9798..>10554)
                     /locus_tag="VIX62_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY5860233.1"
                     /translation="RDLPDAALAHRALQNVERVVVQSLELGSLEPFASAFLPAAPFIE
                     KEGHVTTWEGRGQRLQPIRGAQGISLPDWEIFASMALACGGDLGFETLDELHEEMGSL
                     LAPRSVGVMTVREHEPRAAAGLQLFTYPLLIDDGRLSERAEELEASLGSDPFVEIHPE
                     DAAAAGIADGASAIVRTDAGEATLPARVTDHVARGAVFVPFNQPGFAANTLLSGRMTA
                     PVELTAVDVPEAEDAGSPNAGAEPVGSASGGAA"
BASE COUNT         1972 a         3664 c         3471 g         1447 t
ORIGIN      
        1 tgaggaagca gaagtaatcg aacccgagct ggcgggcggc ctgcccggcg gcgcgccacg
       61 agtcggagcg gatgcgcagc cagaggtcgt cacccgtctt caggtgcgag tcgacgagcg
      121 cgtcgccgag gacctcacgg aaccggtcga gaaggccggt gcgcagggtg tcgcccggga
      181 tctcggcgac gggtgcctcg tcagccgacg acaacggtct cacctccgtc gccggacggc
      241 agcgcggcgg gcggagactc ggatgcgggc gtcctggcag ccgcgtcgct cttcgccggc
      301 gcggacgcca gcagcttcgt gccctcgccg cgccaccgcg cgcccatgtc ctcgttcttg
      361 atccgctgct gcaggagcac gacagcctcg aggagcgcct cgggcctcgg cggacagccg
      421 ggcacgtaga tgtcgacggg gatgatctgg tcaacgccct tcgtcaccga gtagctgtcc
      481 cagtacgggc cgccgcagtt cgcgcacgaa cccatcgaga tgacgtactt cggctcgggc
      541 atctgctcgt acacgcgctt gagggcgggc gccaacttgt cggtgagcgt gccggccacc
      601 accagcaggt cggcctggcg gggcccgtgg gcgaacggga tgacccccag gcggatgaag
      661 tcgttcttgg ccgtggatgc ggcgatgaac tcgatggcgc agcacgcgag gccgaagttc
      721 atgacccata gggagtagcg gcgtccccag ttgaggatga acttcaccgg ctggaccttg
      781 gcgagcgatg tcttctcggt gatcaggccc atcgcagtac ccccttgcgc catgcgtaga
      841 gcagaccgac cgccaagatc ccgatgaaga tggccatctc caccaccgcg gcatacccca
      901 ggctctcgaa cacgcgtgcc caagggaaga ggaacacgct ctcgacgtcg aagatgacga
      961 acaggaaccc gtacacgtag tagcggacct ggctctggga ccagccctca cctacggggt
     1021 cgatgccgca ttcgtaggtg gtgagcttct cgcgattggg cttgtggggg gccaccaggc
     1081 gggccgtgcc gaacgccacg gcgaccaaca gcacgcccgc ggtcaggacc gcgagtacga
     1141 caccgtagtt ggagtagtag tcacccatgc gttgtggtcg gatcctcgag cgcccgtatc
     1201 ctagcagcgc cttcgttggt cctcgctact cacctgcctc tgcgccgcac cggaccgcga
     1261 ggaacctggc cagctggatc agcgcgctct gggcatggcc gtcggggagc cggcgcgcca
     1321 gccccaccgc gcgagccact tcctcggccg tggcccgccg cgcgtgttcg atcgaaccgc
     1381 cggtccgcac gatctccagg gcccggtcga gccgttcccc gtcgggggcg ccgtgcctca
     1441 gcaggctcgc caactcgtcg cggtgggggc cttcgtggag ggcatgcagg acgggcaggg
     1501 tgtagacgcc ctccttcatg tcctggcccg gttccttgcc cagttcgagc tggctggcgg
     1561 tgaaatccat gatgtcgtcg gagagctgga aggccatgcc caaggactcg ccgaaccctt
     1621 cgagcacctc ggtgtgctcg gtggtggcgt ccgagagcat gccgcccagg cggcaggagg
     1681 tggcgatcaa caccccggtc ttgcgccgga tgatgcccag gtagttgtcc tggtcctgct
     1741 cgaccttgcc cgaggaatcc acctcgcgga tctggccgtc gcacagcacg gcgatcgtct
     1801 gggccagcag gcggcagacc tcgggaccca ggtcggcgga cagctccgag gcgcgcgcga
     1861 acaggtaatc gccggtgagg atcgccaccg tgttgtccca gcgggcgttc gcggcgggca
     1921 ccccgtggcg ctcgtcggcc tcgtcgatca catcgtcgtg gtacagggtg gcctggtgga
     1981 ccagttcgat cgccaccgag cccgggatca gcctcggatc ggtggggtcg ccgaagtagc
     2041 ccgacaggag caccaacatc ggtcggaacc gcttgccacc ggccgtgagc aggtacttcg
     2101 ccgtggtggc gagcagctcg gagtcggcgc cgacggcttt cttcagggcg tcctcgacgc
     2161 ggtccagtcg cgcacggatg tccgcttcca gcgcggcgtc cggcgcctcg aggccgggga
     2221 tgactcgacg actcatatcc gtacctttcc gacctcggga ggtctcacca cttgagcacc
     2281 gacgcttgtt ccaagatccc cacgatcagt ccggggaaca cgcccagcgc gatcgtcacg
     2341 acggccggga tcaccaggac gagccggggt gtgagcgcat cgtctcggtc gtactccccc
     2401 tgcggctcct gcatgtacat cacgacgatc acccggaggt agaagaacgc cgccaccacg
     2461 ctggacaaca caccgatcac cgcgagcggc caggcgcccg ccccgatcgc cgaagcgaac
     2521 acccccacct tcgcgatgaa gccggccgtg ggcgggatcc ccgcgaggga cagcatgaag
     2581 atggccatca gtcccgccag caacgggctg cgcttcgaca ggccggcgta ggacgtgatc
     2641 gaggtctgct cctcgccctt ggccgagacc agcatcacca ccccgaacgc ccccagggtc
     2701 atggcggagt acgccaccag gtagaagagc gccgagcgga tcccgaccgc cgtgcccgac
     2761 acgaggccgg tgaggatgaa tccggcctgc gccaccgagg aataggccag catgcgcttg
     2821 atgtcggtct gggcgatcgc cagcaccgag ccgaccacga tcgagagcgc ggccagcacc
     2881 cagatcaccg gctgccagtc ccacgtcagc ggctggaacg ccaccatcag cacccggatc
     2941 agcgcggcga aggccgccac cttcgtcgcc gccgacatat aggccgtgac cggcgtgggc
     3001 gcgccctgat agacgtcggg cgtccacatg tggaacggcg ccgccgacac cttgaacccg
     3061 aagccgatcg ccaggaacgc gaacgacagc agcgccagcg cctggttgcc ggtctgcccg
     3121 ctcatcgagc gcacgatctg gacgatgttc gtcgtgtcga ccgcgccgta ggccatggcc
     3181 acgccgtaca ggaagaacgc cgacgcgaac gcccccagca ggaagtactt catcgcggcc
     3241 tcgttcgcgc gtcgcgcccc ggtgatgccg gtcaagacat acagcgacag cgagagtatc
     3301 tccagtgcaa ggaacatcac gatcaggtcg gcggaggctg cgatcaacgt catgccggcg
     3361 gtggcgaaca gcaccaaggg gtagtactcg ccccgcgccg ccgccgggtc gcgggagaag
     3421 tagtgcgtgc cgtagaccaa tccgaacgcg gcgatcacca gcaggatcgt gcatgccacc
     3481 accgcgaact tgtcggcgct gacgctgccg cccaggacca ccgggtcgcc gtcccagtcc
     3541 cacaggcgca gcgtcgcgat cgcggcggcg agcaggccca gcaggccgat cgccagatgg
     3601 atcgaacgat cgggcttgcg caccaacgcc tcgtacagca ggccgatgat gcccacgccg
     3661 accaggatca actccgggag gaccgggccg aacacgaggg tcggggtctc gatcatccct
     3721 cctcctccgg cggcgacagc tcggagggca cgaccctgag ctgaacgtca ccgacgtcgg
     3781 tggtctgacc ctgcggatcg acgtgtgcga tcaccgcttc ggtcgacggg tcgatgcggt
     3841 cggtgaggat cttcgggtac acgccgaaca ccaggagcag cgcgagcacg ggcgccagca
     3901 ccgccacctc ccgaaggctc acatcgggga gatgccggtg ctcatctctg accggaccga
     3961 aggccatgcg ctggtaggac cacaggaggt agatggcggc cagcaccacc gcgatcgcgg
     4021 cgatcgagcc gaaccagtgg ttcacggcga acgtccccac gatcaccagg aactcaccca
     4081 cgaagctgtt gaggccgggc aagccgacgg aggcgaacac cgcgaacagg aacgcgccgg
     4141 tgagccacgg catcacgctg gcgagcccgc ccatcttcgc gaggtcccgg gtgtgcgttc
     4201 gttcgtacac catgcccacc agcaggaaga gggcgcccgt cgcgaggccg tggttcacca
     4261 tctggagcac gccgccggtg acggcctgct cggtgaacgc gaagatcccc aggacgacga
     4321 accccaggtg ggacaccgag gagtaggcca ccagccgctt gatgtcggtc tggatcagcg
     4381 cgcagaccgc gccgtagacg atgccgatca cggccaggat cgacacgaac gtcgcgaagt
     4441 acttcgaggc gccggggaac aacgccaggt tgaagcggat caggccgtag gtccccacct
     4501 tcaacagcac gcccgccagc agcaccgacc cggcggtggg cgcttccgtg tgcgcgtcgg
     4561 gcagccacgt atggagcggg aacaccggca ccttcacggc gaacgcgatg aagaacccga
     4621 ggaagagcca ccgggcggtc gccaacggga tcgccgcgcc cacctcaccg agctgccgca
     4681 ggtcgaaggt gttcgccccg cccaggacgg cgcccgagcg ggagtacagg aagatgatcg
     4741 agaccaacag gaacgccgag cccgccatcg tgtagaggaa gaacttcacc gcggcataga
     4801 cgcgacgctc gccgccccag ccaccgatca gcaggtacat cggcaccagg atcgcttcga
     4861 agaacaggaa gaacagtagg aggtcgagcg agacgaacga gcccagcacg gccgtttcca
     4921 gcgccagcat cgcgcccatg tagagccgca cgtccttgtc gatcttccac gaagccagga
     4981 ccgccaccgg gaacaggaag gtggtgagca gcaccatcca gatgctgatg ccgtcgatcc
     5041 cgacgaggta ttgcagcccg acgtccttga cccacgtggc ctgctccacc atctggaagc
     5101 cggggtcacc ctgttcgaag cggcccagca ccaccagcga gaccacgaag gtggcaagcg
     5161 ccaccacgag ggtgatccac cgcgcggcgt cgtccttcag ctccttgccg cccaccatga
     5221 tcaccagcag ccccaccaag ggcaggaacg tcacgatcgt gagcagccgg ttcacaggcg
     5281 gaaccccacg tagacgacga tcgcgaccgc cccgacgaac acggccgccg cgtacgtgcg
     5341 gacgaagccc gtctggatgt tgcggcccac acccgccaag cgccggaacg cgctgccgat
     5401 cccgttcacg atgccgtcga tgaacccgac atcgatgcgg taggcggtga aggccgcgat
     5461 cgccttgccg ggcgtgacga tgagggcggc gtagtactcg tcgatgtacc acccattggc
     5521 gaacagccgg tgcaggggcg ccaggcggac ccgcaacgag agccagtcca cccggcccga
     5581 cccgtagacg aaccatgcgg tgcccagtcc gccgaaggtc accagcagcg ccacgacgta
     5641 gaagaccggg gtcgacagtc cctcgctccc ctcgggcacc agccccgaga tcggttccag
     5701 gaaggtggcg agcaagcctt cgggatgggt ggcgagggcc ccgacggtca cggcccccac
     5761 ggcgagcagg acgagcggga tccacatcac cgggggagac tcgtgcgcgt gctcggcggc
     5821 ttcacttcgg gccgtgccgt agaaggccag gaagatcacg cgccccatgt acagcgccga
     5881 gatgaacgcc gcgagggtgc cgaggaggta gtagccccac tcgctgttgg actgggcgat
     5941 ctcgagcacg gcgtccttgg cgaagaagcc cgccaacggc ggcacgcccg agagcgccag
     6001 cgcgccgagc gtgaacgtga tcgccgtgta gggcatgcgc cgccacaggc cgcccatcac
     6061 cttcagatcc gtctcctcgt gcatgccgtg catcaccgag ccggcgccga ggaacatgag
     6121 cgccttgtag aaggcgtggg ccaccaggaa gaagatcgcc gccgagtacg ccttcagtcc
     6181 cgccgccagg aacatgaacc cgagctggct gatcgtggag taggccagca cccgtttgat
     6241 gtcgtcctgg cccagcgcac acgtacccgc gtagatcgcg gtcaccaggc cggtgacggt
     6301 caccaccgtc agcgccaccg gcgacagatc gaacaccggg tgcatccgca ccaccaggta
     6361 gaccccggcg gtcaccatgg tggccgcgtg gatcagggcc gagaccggtg tggggccggc
     6421 catcgcgtcg gggagccaca cgtgcagcgg cagctgcgcc gacttgccca cggcgccggc
     6481 gaacaacagc aacgagatca ccgtggccgc cgacttcgtc agcgtcgagc ccgcggcgcc
     6541 gaagatgacc tcgtagtcca gcgtgccgaa cttcatgacc accacggcga gccctacgag
     6601 catcaacgtg tcgcccaccc gtgtggtgac gaacgccttc ttcgcggcgt tggcgttcgc
     6661 cgtcttatcg aaccagaacc cgatcagcag gtaggagcag agccccacgc cctcccagcc
     6721 caggtacagg accagcaggt tggcacccag gaccagcatc agcatgaaga acacgaacag
     6781 gttgaggtag gcgaagaagc gcccgaaccg gccgtcgccg tccatgtagc cgatcgcgta
     6841 gacgtggatc agggtcccga tgccggtcac caccaggatc atcgtggccg acagcgcgtc
     6901 gagccgcagg tcgaagttca cgcgcaacga gcccacggag atccagtccg acaggtgctt
     6961 gaggaacagg cgctcgcctt cgggcgcgtc gagcagcccc ttcaccgcga gcaccgccac
     7021 cgcgaacgac agcgccatca tcacgaccgc gagccacccc gcggccttgc ccagccgctt
     7081 gccgacgaac aggttgatca ccgcgcccac gagcggcagc agcgggacca gccacaagac
     7141 ctgcatcgcc tggttgggat cgggcacgag tcccacgctc tcggctacca ccggagctcc
     7201 ctcgcgtcgt cgacgttgac ggtggcgcgg cgccggtaga tcgacacgat gatcgcgagg
     7261 cccaccacga cctcggcggc cgccaccacc atggagaaga aggccagcac ctggccccgc
     7321 agatcgccgt tcatgcggct gaacgccacc agcgccaggt tcacggcgtt cagctggagc
     7381 tccaccgaca tgaagatcag cagcgcgctc ttgcggatca gcacgcccac cgagccgatc
     7441 gcgaacagga tgcccgacag catcacgaag taggcgatcg gcgtcctcat cgaggctccc
     7501 ccgcctcgag cggcgctccg ggcttgggcg cgcccgggtt cgtgtggccg agcgcgtcga
     7561 ggtcggcgcg gtgctcgtcg atctccgcct gcgtgatcgc gcgcgacttc cgctgcgcca
     7621 gcaccatcgc ggccaccgcc gcgatgatca acaacaccga ggtcacctcg aacgggaaga
     7681 agtagtcgcg gaacaacacg gtggccaggg cctgggcgtt gccgccctga ttcacgacgt
     7741 cgaacccgtc gggcgctcgc gtcgcgaacc cgatcccggc gcgcaacgcg aacccgatct
     7801 ccagcacgaa ccccaccgcc aggatcaccg cgagggggcg ttgcccgatc aacggttcgt
     7861 gcacatcgtc gccgcgatcg atgcccagga gcatgatcac gaacaggaac agcaccatga
     7921 tcgcgcccgc gtacacgatg atctggaccg cgaacaggaa cggcgcgtcg agcagcaggt
     7981 acatcaccgc gatcgtgaag aagttcacga tgaggaacag cgccgcgtgc acggcgttgc
     8041 gcatcacgag catcccgatg gccgaggcca ccgagaccgg cgcgaacacc cagaacacga
     8101 cgttgtcgag gggcaccggt tcctagccct cccggtccac ggggtcggtg cccagctccg
     8161 gcggcggggt gaggagctgc tccttggtcc agatcgcctc ttcgcggctg gtgaatccca
     8221 tctcgaagta gctcgtgagc gtgagcgccc gcgtcggaca cgcctcgacg cacaggccgc
     8281 acatgatgca gcgcaggtag ttgatctgat agtccttcgc gtaccgctcg ccgggactgg
     8341 tgggcgcctc aggatcgttg tccgctcctt cgacccagat cgcatccgcg gggcaggcgt
     8401 aggcgcacaa ctcgcacccg atgcacttct ccaggccgtc ctcgtgcttg ttgagacggt
     8461 ggcggccgat gtgggaccgc tccgggatcg gcgcgatctc cttcgggtac tgctcggtgt
     8521 tgtcgggccg gaaggtctgc ttgaacaccg tgcccatgcc tttggcgatc gccacggtgg
     8581 agtcccagac cccgccgcgc tcacgaggat cgcggtcgtc ggtcatgcgc ccacctcctt
     8641 gcgggatcgc gcgctgcctc gggagaacaa cggcgcgagc aacagggcca gcgcgagcgt
     8701 gccgcacatg accgcgaaca tcaggaactt cccttggttg ccgtactcct gcgggatcac
     8761 caccacgagc ccggtgacca tgacccacag caggccgaac gggatcaata ccttccagcc
     8821 gaactgcatc agccggtcgt agcgcatgcg gggcagcgtg gcccgcacca ggatcatcac
     8881 gtagatcacc gcggtgatct tcagcaggaa ccacagcagc gggccgatcc acgacagcgg
     8941 cccgtcgaac cacggcccgt gccagccacc caggaacaac gtgacgccga cggccgcgat
     9001 cgtcacggtg ctgacgtact cacccaggaa gaacatcgcg aacttgatcc cggagtactc
     9061 ggtgtggaag ccggccacca gctcggactc ggcttcggcg aggtcgaacg gcggacggtt
     9121 cgtctcggcc agcgcacaga tgaagaacac gacgaaggcg gggaactgcg ggaagatgtt
     9181 ccacgccggc acgccgaaga gggtggcgtc gtgccagtgg agcgcgaagt tgcccgactg
     9241 ctgcgcgacg atctccgaca tggacaggtg gccgctccac atgatcaccg ccaccagggc
     9301 cagacccatg cccacctcgt agctgatcat ctgcgcgctc gagcgcacgg agccgagcag
     9361 cggatagttc gaaccgctgg accagccggc caacaccacg ccgtagaccc cgatcgaggt
     9421 catggcgagc acccacagga tgccgatgtt gaggtcggtg atctggaacg gcaccacccg
     9481 gccgaacagc tccacgggct cgccgaacgg gatcgtgcag aacgccaaga acccggggaa
     9541 catcgccagg atcggcgcga tcaggtagac gggccggtcg gcgttcgtgg gcgtgatgcc
     9601 ttccttgaag aacagcttga tgccgtcggc cagcgtgacg agcatgccgc ggggaccggc
     9661 gcgcatcggg cccacccggg tctgcatgtc ggcgatcacc ttgcgttcca tccagatgta
     9721 gagcagcacg gcgatcagca gcacgaagaa gaccagcacc acccgcacca ccatgaccga
     9781 ccagtcgacc cagtccatca ggcagcacct cccgaagcgc tgcccacggg ttcggccccg
     9841 gcgttcggag atccggcgtc ctcggcctcg gggacgtcga cggccgtgag ctcgaccggt
     9901 gcggtcatac ggcccgagag cagggtgttg gccgcgaacc cgggctggtt gaacggcacg
     9961 aagacggcgc ccctcgcgac gtggtcggtg acacgcgccg gcaacgtggc ctcacccgcg
    10021 tcggtgcgga cgatcgccga tgcaccgtcg gcgatcccgg ccgccgcggc gtcctcgggg
    10081 tggatctcca cgaagggatc ggagccgagc gaggcctcga gttcctcggc gcgctcggac
    10141 aaccggccgt cgtcgatcag gagcgggtag gtgaagagct ggagtcccgc ggcggcacgt
    10201 ggttcgtgct ccctgacggt catgaccccg accgatcgcg gcgccaacag cgagcccatc
    10261 tcctcgtgca actcgtccag cgtctcgaaa ccgagatcgc cgccgcacgc cagcgccatg
    10321 ctggcgaaga tctcccagtc gggcaggctg atcccctggg cgccgcggat cggctgcagg
    10381 cgctggcccc ggccttccca cgtggtgacg tgcccttcct tctcgatgaa cggggcagcg
    10441 ggcaggaacg cgctggcgaa gggttccagc gaaccgagct ccagcgactg caccacgacc
    10501 cgttcgacgt tctgcagcgc gcgatgcgcg agcgcggcgt cgggcaggtc ccgc
//
LOCUS       DATDBZ010000335         2022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1333551, whole genome shotgun sequence.
ACCESSION   DATDBZ010000335 DATDBZ010000000
VERSION     DATDBZ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333551"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VIX62_08115"
     CDS             <1..227
                     /locus_tag="VIX62_08115"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEY5860234.1"
                     /translation="LLLTYNLVSLFAGFMPVPGGMGVAEAGYTAALVAFGVPHTAALS
                     TAMAFRLVTYYLPPIWGGVAMKWLRGREYV"
     gene            332..1717
                     /locus_tag="VIX62_08120"
     CDS             332..1717
                     /locus_tag="VIX62_08120"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEY5860235.1"
                     /translation="MVHKQVSKDDPEAGGAIAPAYTHRLDLEPIPKFVMPEHDARPET
                     VYRLIHDELLLDGSSRLNLATFVTTWMEPEADKLMAEAFDKNMIDKDEYPQTAEIERR
                     CVNMVAHLFNAPKDGDAVGASTIGSSEAVMLGGLAMKWNWRTRREAAGKSTDKPNLIL
                     GSNVQVVWEKFCRYWDVEARYIPLHEGRYVISPEEAVERIDENTIGVVAILGTTYTGE
                     FEPIEAIHDAVVASNAETGFDVPVHIDAASGGFVAPFLHPKVKWDFRLPMVKSINVSG
                     HKYGLTYPGVGFVVWRSSDDLPDDLVFHVNYLGGDMPTFTLNFSRPGNQIVGQYYNFL
                     RLGMAGYRRIMETLRDLAVHTSGRIAEMGPFELVSDGTAIPVFAFALKEGAPYTVFDL
                     SERLRIGGWQVPAYTMPDAATNVAVMRVVVREGFSYDLADDLIDGLKAATEFLTQNPP
                     RQAAKPGFSHT"
     gene            1720..>2022
                     /locus_tag="VIX62_08125"
     CDS             1720..>2022
                     /locus_tag="VIX62_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860236.1"
                     /translation="MRAHSLVRIPETWVLEEQRTIGPFVTRERYRLPDGSHRTWSSRR
                     RRKTGRTEERRSRPPHPWWRPKALGWWIAALFMVGSSLFALGSFPPYANGTNPTVVG"
BASE COUNT          348 a          690 c          656 g          328 t
ORIGIN      
        1 aactgctcct gacctacaac ctggtgagcc tgttcgcagg cttcatgccg gtgccgggcg
       61 ggatgggcgt ggccgaggcc ggctacaccg cggccctggt cgcgttcggc gtcccgcaca
      121 cggcggccct gtccacggcc atggcgttcc ggttggtgac gtactacctg cctccgatct
      181 ggggcggcgt ggcgatgaag tggctccgcg gtcgcgaata cgtgtgagca cgcccgcgtc
      241 gctccaaggc gtctccgtgc accgtgcggt gcagtagcct tcgacccgag agtcttcata
      301 gctccgtccg catgcagtcg aggaggaaca gatggtgcac aagcaggttt ccaaggacga
      361 tcctgaagca ggaggcgcca tcgcacccgc atacacccat cggctcgacc tagagccgat
      421 cccgaagttc gtcatgcccg agcacgatgc acgcccggaa accgtctatc ggttgatcca
      481 cgacgagctc ctgctcgacg gaagctcccg cctgaacctc gccacgttcg tcaccacctg
      541 gatggagccc gaggccgaca agctcatggc cgaggcgttc gacaagaaca tgatcgacaa
      601 ggacgagtac ccgcagaccg ccgagatcga gcgtcgctgc gtgaacatgg tggctcacct
      661 gttcaacgcg cccaaggacg gcgacgccgt cggcgcctcc acgatcggtt ccagcgaagc
      721 cgtcatgctc ggtgggctcg cgatgaagtg gaactggcgg acccgtcgcg aggccgccgg
      781 caagtccacc gacaagccca acctcatcct cggatccaac gtccaggtcg tgtgggagaa
      841 gttctgtcgg tactgggacg tcgaggcgcg gtacatcccg ttgcacgaag ggcgatacgt
      901 catctccccc gaggaagccg tggagcgcat cgacgagaac accatcgggg tcgtcgccat
      961 cctgggcacc acctacaccg gcgagttcga gccgatcgaa gcgatccacg acgccgtggt
     1021 ggcgagcaac gccgagaccg ggttcgacgt cccggtccac atcgatgccg caagcggtgg
     1081 gttcgtggcc ccgttcctgc atcccaaggt caagtgggac ttccgcctgc ccatggtgaa
     1141 gtcgatcaac gtctccggcc acaagtacgg cctcacctat ccaggcgtcg gcttcgtcgt
     1201 gtggcgctcg agcgacgacc tgcccgacga cctcgtcttc cacgtgaact acctgggcgg
     1261 cgacatgccc acgttcacgc tgaacttctc acggcctggc aatcagatcg tgggccagta
     1321 ctacaacttc ctgcgcctgg ggatggccgg gtaccggcgg atcatggaaa cgctacgcga
     1381 cctcgccgtg cacacgtccg gtcgcatcgc cgagatgggg ccgttcgagc tcgtgagcga
     1441 tgggaccgcg atcccggtgt tcgccttcgc cttgaaggag ggcgcgccct acacggtctt
     1501 cgacctctcc gagcgcctgc ggatcggcgg ctggcaggtg ccggcctaca ccatgcccga
     1561 cgccgcgacg aatgtggcgg tcatgcgcgt ggtggtgagg gaggggttca gctacgacct
     1621 cgccgacgac ctgatcgacg ggctcaaggc cgcgaccgag ttcctgaccc agaacccccc
     1681 acgacaggcc gcgaagccgg gcttcagcca cacctagacg tgcgcgccca ctcgctggtt
     1741 cgcatccccg agacgtgggt cctggaggaa cagcggacga tcgggccgtt cgtcacccgc
     1801 gagcgctatc ggctgccgga tggctcgcac cggacgtgga gctcgcgccg ccggcgcaag
     1861 acgggccgga cggaggaacg gcgcagccgt cctccccacc cctggtggcg cccgaaagcg
     1921 ttggggtggt ggatcgcggc cctgttcatg gtcgggtcct cgctcttcgc cctggggtcg
     1981 ttcccgccct acgcgaacgg gacgaacccc acggtcgtgg gg
//
LOCUS       DATDBZ010000336         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_514630, whole genome shotgun sequence.
ACCESSION   DATDBZ010000336 DATDBZ010000000
VERSION     DATDBZ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514630"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VIX62_08130"
     CDS             <1..547
                     /locus_tag="VIX62_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY5860237.1"
                     /translation="MGADSAHADELVKHLDDLDLRTHPSVFRKGTMACTGIEFCKLAI
                     GETKTRAQWLYAELEQRLPDFDEDIRIHVNGCPNSCTRFQVADIGLMAALQVRPDGTK
                     SDAFLVHLGGTMGEGAAFGRKVKGVKVYAEDAADYLELLLSRYQLARTSDDSFTSYVN
                     ALDADGLARFAAPAVAHGELR"
     gene            544..690
                     /locus_tag="VIX62_08135"
     CDS             544..690
                     /locus_tag="VIX62_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860238.1"
                     /translation="MSVRAVPFYCPFCGEQDIRPDDAGTAAWRCESCERRFELTLTAI
                     GGDA"
     gene            690..1538
                     /locus_tag="VIX62_08140"
     CDS             690..1538
                     /locus_tag="VIX62_08140"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY5860239.1"
                     /translation="MADADPHAPWHEPAMPVVEEETAWVHEAAERFEDAPGEEILAWG
                     LERFHPKMAISAAGGVDGMVLIDMAWRLNPDIRVFTLDTGRLPPETYALFEEIRDRYG
                     IAVEFEYPDTTAVATLVNREGPNLMYRGVDLRLACCTVRKVEPLKRKLATLDAWVAGL
                     RREQWASRKNIAKVELDRDHGGIVKLNPLADWTLDQVWAYVHEHEVPYHELFDHGYTS
                     IGCAPCTRPVIEGEPERAGRWWWEEDTAKECGIHCSVELTGTKSDREKAEEAALVAAR
                     ERDGDR"
     gene            1538..2194
                     /locus_tag="VIX62_08145"
     CDS             1538..2194
                     /locus_tag="VIX62_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEY5860240.1"
                     /translation="MPFGYPVMLELAGRRCVVIGSLAVREGKVEGLLAAGATDILVVA
                     SAPDPRLSDLEELPEVVVERRTWRANDLNGAFVVVASSADAGEREAIAREARCRRALV
                     NVMDDIPNCDWSAPAIVRRGELVLSIATGGGSPALAKQLRAALSEEFGEEWSGVVSVL
                     RAVREETTPSLPDVATRAQRWSDALDLDEAATLVREGRSEELASRLRTRLLDPEGART
                     "
     gene            2194..2280
                     /locus_tag="VIX62_08150"
                     /pseudo
     CDS             2194..2280
                     /locus_tag="VIX62_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018216689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
BASE COUNT          373 a          736 c          843 g          328 t
ORIGIN      
        1 catgggcgcc gactccgctc acgccgacga gctggtgaag cacctcgacg atctcgacct
       61 gcgcacgcat cccagcgtgt tccgcaaggg gacgatggcg tgcacgggga tcgagttctg
      121 caagctggcg atcggggaga cgaagacgcg cgcgcagtgg ctctacgccg agctcgagca
      181 acggctgccg gacttcgatg aggacatccg gatccacgtg aacggatgcc cgaactcatg
      241 cacacgcttc caggttgccg atatcggatt gatggcggcg ttgcaggttc ggcccgacgg
      301 cacgaagtcc gatgccttcc tcgtgcacct ggggggcacg atgggcgagg gggccgcgtt
      361 cgggcggaag gtgaagggcg tgaaggtgta cgccgaggat gccgccgact acctcgaact
      421 gctgctgtcg cgctaccaac tggcgcggac gagcgacgat tcgttcacgt cctacgtcaa
      481 cgccctcgac gccgatggac tcgcacggtt cgcggcaccc gcggtggcgc acggagagct
      541 ccgatgagcg tgcgcgccgt gccgttctac tgcccgttct gcggcgagca ggacatccgt
      601 cccgacgacg ccggcaccgc ggcatggcgg tgcgagtcgt gcgagcgccg gttcgagctg
      661 acgctcaccg cgatcggagg tgacgcctga tggccgacgc cgacccgcat gcaccgtggc
      721 acgagccggc gatgccggtc gtcgaggagg agaccgcgtg ggtgcacgag gccgccgaac
      781 ggttcgagga cgcgccgggc gaggagatcc tggcctgggg cctggaacgc ttccacccga
      841 agatggcgat cagcgcggcc ggcggcgtgg acggcatggt gctgatcgac atggcatggc
      901 gcctcaaccc ggacatccgc gtgttcacgc tggacaccgg ccgtctgccg ccggagacct
      961 acgcgctgtt cgaagagatc cgagaccgct acgggatcgc ggtggagttc gaatatccag
     1021 acaccaccgc ggtggccacg ctggtgaacc gcgagggccc gaacctgatg taccggggcg
     1081 tggacctgcg gctcgcgtgc tgcaccgtgc gcaaggtgga gcccctcaag cgcaagctcg
     1141 cgacgctcga tgcgtgggtc gccggcctcc ggcgcgagca atgggccagc cggaagaaca
     1201 tcgcgaaggt ggagctcgac cgcgaccacg gcggcatcgt gaagctcaac cccctggcgg
     1261 attggaccct cgatcaggtg tgggcgtacg tgcacgaaca cgaggtgccc taccacgagc
     1321 tgttcgacca cgggtacacc tcgatcgggt gtgcgccgtg cacgcgcccc gtgatcgagg
     1381 gcgaacccga gcgcgccgga cgctggtggt gggaggaaga caccgccaag gaatgcggca
     1441 tccactgcag tgtggaactc acgggcacga agtccgaccg cgagaaggcc gaagaagccg
     1501 ccttggtggc cgcacgtgaa cgggacggtg accgctgatg ccgttcggat atccggtgat
     1561 gttggagctc gccggccgtc gctgcgtggt gatcggctcg ctcgccgtgc gcgagggcaa
     1621 ggtcgaagga ctgctggccg ccggggccac cgacatcctc gtggtcgctt cggcgcccga
     1681 cccgcggctg agcgacctgg aggaactgcc cgaggtcgtg gtggagcgcc ggacctggcg
     1741 ggccaacgac ctcaacggcg ccttcgtggt cgtggcgtcg agcgccgacg cgggtgagcg
     1801 ggaggcgatc gcccgggaag cccgctgtcg tcgcgcactg gtgaacgtga tggacgacat
     1861 ccccaactgc gactggtcgg cacccgcgat cgtgcgtcgc ggcgagctgg tgctgtcgat
     1921 cgccaccggt ggcggctcgc ccgcgttggc gaagcaactc cgtgcggcgc tgtcggagga
     1981 gttcggcgag gaatggtcgg gggtggtctc ggtgctgcgc gcggtgcgag aggagacgac
     2041 gccttccctg ccggacgtcg cgacgcgagc ccaacgctgg agcgacgcgt tggacctgga
     2101 cgaagccgcc acgctggtgc gtgaaggtcg cagcgaggaa ctcgcgtccc gactccggac
     2161 ccgcctcctc gatcccgaag gcgcccggac atgaccacgc gcgcgggccg ggtgtcgctg
     2221 gtgggcgcag gaccgggcga tcccgagttg atcacggtga agggagcccg cctcctggcc
//
LOCUS       DATDBZ010000337         3715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_92810,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000337 DATDBZ010000000
VERSION     DATDBZ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92810"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VIX62_08155"
     CDS             <1..543
                     /locus_tag="VIX62_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5860241.1"
                     /translation="EGATILEVGGGVGEIQLELLTRGAAATVNLELSDAYEEQAASLM
                     RLKGVAGRVTRVVGVDLAESGQYVESADVVILHRVVCCYPDFEHLLGAAADHARTALV
                     FSHPPRTWLTRATVRLSNTWMAVLGRRYRGFVHSPDAMIDVLRMHGMTVHRRDRAARW
                     HIAAAVREPAPIPTTTGATG"
     gene            complement(592..1014)
                     /locus_tag="VIX62_08160"
     CDS             complement(592..1014)
                     /locus_tag="VIX62_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012038527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEY5860242.1"
                     /translation="MRANAILFWILAGFFGLSAAVYATWSWLDEQDPGLEPAGTVALT
                     LTTVLAAFIAFYLGRVHSAQGAELPEDRVDANIDDGDAELGFFSPWSWWPIILAGGAA
                     LGFLGLAIGFWITFIAVPIVVVALVGWVYEYYRGNFAR"
     gene            complement(1015..2748)
                     /gene="ctaD"
                     /locus_tag="VIX62_08165"
     CDS             complement(1015..2748)
                     /gene="ctaD"
                     /locus_tag="VIX62_08165"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY5860243.1"
                     /translation="MSTTTAPARPASTSPFGTPKVERKGNILVRWITSTDHKVIGYLY
                     LITAFIYFCIGGVMALIIRAQLFEPGLEIVQTREQYNQLFTMHGTIMLLMFATPLFAG
                     FANVLMPLQIGAPDVAFPRLNAFAYWLFNFGSLIAVAGFFTPQGAASFGWFAYQPLAS
                     TTFSPGIGGNLWMLGLGLSGFGTILGAVNFITTIITMRAPGMTMFRMPIFTWNTLVTS
                     ILVLMAFPVLAAAILAAGADRIFGAHIYDPANGGVILWQHLFWFFGHPEVYIIALPFF
                     GIVSEIFPVFSRKPIFGYKTLIYATIAIAALSVTVWAHHMYVTGSVLLPFFALMTMLI
                     AVPTGVKIFNWIGTMWRGSITFETPLVWSLGFLITFVFGGLTGVILASPPLDFHVSDT
                     YFVVAHFHYVVFGTVVFAMFAGFYFWWPKWTGKMLNETLGKWHFWLLFIGFHTTFLIQ
                     HWLGVVAMPRRYYSYLPEDNITWMNQLSTIGAGILAVSMIPFFLNVYITARRAPKVTV
                     NDPWGYGRSLEWATSCPPPRHNFTSIPRIRSEAPAFDLNHPEAGIPVGIGPAKDAPQA
                     PVYDAATKEVK"
     gene            complement(2755..3648)
                     /gene="coxB"
                     /locus_tag="VIX62_08170"
     CDS             complement(2755..3648)
                     /gene="coxB"
                     /locus_tag="VIX62_08170"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY5860244.1"
                     /translation="MRHNRRLRWAAIPIAASLSLVLAGCTQAQLNGFLPGFEAGEPPV
                     TNHTERVSGLWVTSWIVLLVVGVITWGLTIWAVIAYRRRKGQTGLPVQLRYNMPIEVF
                     YTIVPLILVLGFFAFTARDQQVIEQRFAEDQIDVKVEVIAKQWAWDFNYVNEDVYSPG
                     IQGQISDEGPAGSLVQSELPTLYLPVGKNVEIELEARDVIHSFWVVDFLYKKDMFPGK
                     TNYMSFVPEREGTYEGKCAELCGEYHSMMLFNVQVVSEAEYEEYIESLRDLGQEGQLS
                     NEYDRRQNLPGTGEPELKEEE"
BASE COUNT          688 a         1157 c         1289 g          581 t
ORIGIN      
        1 gagggggcga ccatcctcga ggtcggcggc ggtgtcggtg agatccagct ggagctcctc
       61 acgcgcggtg cggccgccac ggtgaacctg gagctgtccg acgcgtacga ggagcaggcg
      121 gcgtcgttga tgcgcctcaa gggcgtcgca gggcgagtga cccgcgtcgt gggcgtcgac
      181 ctcgcggagt ccggccagta cgtggaatcc gccgacgtcg tcatcctgca tcgggtcgtg
      241 tgctgctatc cggacttcga gcacctcctg ggcgccgccg ccgatcacgc ccgaacggcg
      301 ctcgtgttca gtcacccacc gcgcacctgg ctgacgcggg cgacggtccg cctcagcaac
      361 acgtggatgg cagtgctcgg tcgccgatac cgaggcttcg tgcactcccc cgacgcgatg
      421 atcgacgtcc tccggatgca tggcatgacc gtgcaccgac gcgaccgcgc ggcgcgctgg
      481 cacatcgctg cggctgtgcg cgaaccggct ccgattccga cgacgacggg agcgaccggc
      541 tgaacgacga agccccgaca ccgggcggtg ccggggctca cacgtcgacg ctcagcgggc
      601 gaagttgccg cggtagtact cgtacaccca gcccacgagg gcgaccacga cgatcggaac
      661 cgcgatgaac gtgatccaga acccgatggc cagcccgagg aatccgagcg cggcgccacc
      721 ggcgaggatg atcggccacc agctccacgg gctgaagaat ccgagctcgg cgtcgccgtc
      781 atcgatgttc gcgtccacgc ggtcttcggg cagctcggcg ccctgcgcgc tgtgcacgcg
      841 gcccaggtag aaggcgatga atgcggccag gacggtggtc agcgtcaggg cgacggtgcc
      901 cgccggctcc agaccgggat cctgctcgtc gagccaggac cacgtcgcgt acaccgccgc
      961 tgataggccg aagaacccgg cgaggatcca gaacaggatg gcattggcgc gcatttactt
     1021 gacttccttc gtcgccgcgt cgtagaccgg tgcctgcggc gcatccttcg ccgggccgat
     1081 gcccaccggg atgcccgcct cggggtggtt gaggtcgaac gccggcgctt cggagcggat
     1141 gcgcgggatc gacgtgaagt tgtgccgcgg cggcgggcag ctcgtggccc actcgagcga
     1201 ccggccgtag ccccacgggt cgttgacggt caccttcggt gctcgacgcg cggtgatgta
     1261 gacgttcagg aagaacggga tcatcgagac ggcgaggatg ccggcgccga tcgtggacaa
     1321 ctggttcatc caggtgatgt tgtcctcggg caggtacgag tagtaccgac ggggcatcgc
     1381 cacgacgccc agccagtgct ggatcaggaa cgtcgtgtgg aagccgatga acagcagcca
     1441 gaagtgccat ttgcccagcg tctcgttgag catcttgccg gtccacttcg gccaccagaa
     1501 gtagaagccc gcgaacatcg cgaacaccac cgtgccgaag acgacgtagt ggaagtgcgc
     1561 caccacgaag tacgtgtcgg acacgtggaa gtcgagcggc ggcgacgcga ggatcacacc
     1621 ggtcaggccg ccgaacacga acgtgatgag gaagcccagc gaccacacca gcggcgtctc
     1681 gaacgtgatg gagccgcgcc acatcgtgcc gatccagttg aagatcttca cgccggtcgg
     1741 aaccgcgatg agcatcgtca tcagcgcgaa gaacggcagc agcaccgagc cggtgacgta
     1801 catgtggtgc gcccagaccg tgaccgacag ggccgcgatc gcgatcgtgg cgtagatcag
     1861 cgtcttgtag ccgaagatcg gcttgcggct gaagaccggg aagatctccg acacgatgcc
     1921 gaagaacggc agcgcgatga tgtagacctc gggatgcccg aagaaccaga acaggtgctg
     1981 ccacaggatg acgccgccgt tggcgggatc gtagatgtga gcgccgaaga tgcggtccgc
     2041 gccggcggcg aggatcgcgg cggccagcac cgggaaggcc atgagcacga ggatcgacgt
     2101 gacgagcgtg ttccacgtga agatcggcat gcggaacatg gtcatgccgg gcgcgcgcat
     2161 ggtgatgatc gtcgtgatga agttcacggc gcccaggatc gtgccgaagc ccgagaggcc
     2221 caggcccagc atccagagat tgccgccgat gcctggagag aacgtcgtgg acgcgagtgg
     2281 ctgataggcg aaccacccga acgaggcggc gccctgcggc gtgaagaagc cggcgacggc
     2341 gatcagcgag ccgaagttga agagccagta cgcgaacgcg ttgaggcgcg ggaacgccac
     2401 atcgggcgcg ccgatctgca gcggcatgag gacgttcgca aagccggcga agagcggcgt
     2461 cgcgaacatc agcagcatga tcgtgccgtg catcgtgaac agctggttgt actgctcgcg
     2521 ggtctggacg atctcgaggc ccggctcgaa cagctgcgcg cggatgatga gggccatcac
     2581 gccgccgatg cagaagtaga tgaacgcggt gatcaggtag aggtacccga tgaccttgtg
     2641 gtcggtggag gtgatccacc ggacgaggat gttgcccttg cgctcgacct tgggagtgcc
     2701 gaacggcgag gtgctcgcgg gacgagcggg tgcggtcgtg gtgctcatcg cggcttactc
     2761 ttcctctttc agttccggct cgcccgttcc gggcaggttc tggcgacggt cgtactcgtt
     2821 ggagagctgg ccctcctggc ccaggtcgcg caacgactcg atgtactcct cgtactcggc
     2881 ctcggagacc acctggacgt tgaagagcat catcgagtgg tactcgccgc agagctcggc
     2941 gcacttgccc tcgtaggtgc cctcacgctc gggcacgaac gacatgtagt tcgtcttgcc
     3001 cgggaacatg tccttcttgt agaggaagtc gacgacccag aacgagtgga tgacgtcacg
     3061 cgcctccagc tcgatctcga cgttcttgcc gacgggcagg tagagcgtcg ggagctccga
     3121 ctggacgagc gagcccgccg gtccctcatc ggagatctgg ccctggatgc ccggcgagta
     3181 gacgtcttcg ttgacgtagt tgaagtccca cgcccactgc ttcgcgatga cctcgacctt
     3241 gacgtcgatc tggtcctccg cgaagcgctg ctcgatcacc tgctggtcgc gtgcggtgaa
     3301 ggcgaagaac ccgagcacga ggatcagcgg cacgatcgtg tagaagacct cgatcggcat
     3361 gttgtaccgc agctgcaccg ggaggccggt ctggcccttg cggcggcgat aggcgatgac
     3421 ggcccagatg gtgaggcccc aggtgatgac gccgacgacc agcagcacga tccacgacgt
     3481 gacccagagg cccgacacgc gctcggtgtg gttcgtgacc ggcggctcgc ctgcttcgaa
     3541 ccccgggagg aatccgttca gctgggcctg cgtacatccg gcgaggacga ggctgagcga
     3601 cgctgcgatc gggattgcag cccatcggag acggcgattg tggcgcaccg gtgacctttc
     3661 gggagactcg aaggcgcttc tcagcgaagc gcggtgatcg atgcctagcc tactc
//
LOCUS       DATDBZ010000338         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2970037, whole genome shotgun sequence.
ACCESSION   DATDBZ010000338 DATDBZ010000000
VERSION     DATDBZ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2970037"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VIX62_08175"
     CDS             complement(<1..292)
                     /locus_tag="VIX62_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5860245.1"
                     /translation="MNGADLASTFPELLARTVASRPEHLAVITGDESVTYDELDRRSA
                     EMAHALLAAGAGKGTRIGLLAPDGVLWLTTFYAALRIGALITPISTLAAPPEL"
     gene            complement(300..845)
                     /locus_tag="VIX62_08180"
     CDS             complement(300..845)
                     /locus_tag="VIX62_08180"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5860246.1"
                     /translation="MVAVPMPGPGFMQVCWLVPDLEAAIDTWATTASVGPFFWFDGVP
                     FEDGRHRGVPAEYPASTAAIAYSGDVQIELVCQENDAPGVFRDVFPLGEHGLHHLARI
                     CHDYEAERDAMVAQGHEVAFEGTVGGSRTCWIDTTATLGFMIELLEPSDARDGWFAHM
                     RKAAERWDGGDRIVGRPAADR"
     gene            complement(845..1690)
                     /locus_tag="VIX62_08185"
     CDS             complement(845..1690)
                     /locus_tag="VIX62_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY5860247.1"
                     /translation="MSDVTAAPCRPVTAEEIEHLNELGWVQLKAFVDPDVIGGVLELA
                     REAMGDDGDGNPIPPGFEQAAADGEFGLDYFNAHLSFGMSNPVLRPLLDDVGKSARLL
                     QRRFVAPGEPMGVRHYSDFFAPKLPSASRSLHRGNGPTAFHQDFITFSVDRSGGLTFW
                     FPLESYGPDAGTMSFVNGSHRYGVLGDYTTYGDRDALEVFPELGELPISDPITYELGD
                     ITVHTHLTVHGAGANVTDRPRWAYIVVTQPADACWNGSPCPNFSTKRMTQWEGLGDER
                     FPVIG"
     gene            complement(1687..>2458)
                     /locus_tag="VIX62_08190"
     CDS             complement(1687..>2458)
                     /locus_tag="VIX62_08190"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5860248.1"
                     /translation="CVREVEWAAEAGLKGINLPAPRGDFPMLNEPVWERLWSACEATG
                     MSLNTHGGGGEHYPYKGDGAQSMYMMETTWRTRRGVWVMILGGVFERHPGLKLVMTEQ
                     WMDWAVQVIADLDGLYTGPAGAHLRKTLPRQPSEYFRENCYIGASFLSNWEAKLGIEH
                     DLVANTMWGDDYPHAEGTWPHTREAMRFTFSDVDERHVRQYLADTAIHVYGLDRPKLE
                     AVAAEIGPTGEEMATPYSPGDDEALGLYAFRTGPGIFA"
BASE COUNT          379 a          842 c          850 g          387 t
ORIGIN      
        1 cgagttccgg gggtgcggcc agtgtgctga tcggggtgat cagcgccccg atccggagcg
       61 cggcgtagaa cgtcgtcagc cacaggacgc catcgggagc cagcaacccg attcgcgtgc
      121 ccttgcccgc gccggcggcg aggagcgcgt gggccatctc cgccgagcgg cgatcgagct
      181 cgtcgtaggt gacggactca tcgcccgtga tcaccgcgag gtgctcgggg cgcgaggcca
      241 cggttcgcgc cagcagctcg ggaaaggtcg atgccaggtc cgccccgttc atctcgccgt
      301 cagcgatcgg cagccgggcg gccgacgatg cggtcgccgc cgtcccaccg ctccgcggcc
      361 ttgcgcatgt gggcgaacca gccgtcccgc gcgtcgctgg gctcgaggag ctcgatcatg
      421 aaaccgagcg tcgccgtggt gtcgatccag caggtgcggc tgccgccgac ggttccctcg
      481 aacgcgacct cgtggccctg cgccaccatc gcgtcgcgct cggcctcgta gtcgtggcag
      541 atgcgagcga ggtggtgcag gccgtgctcg ccgaggggga acacgtcacg gaacaccccg
      601 ggggcgtcgt tctcctggca gacgagctcg atctgcacgt ccccgctgta ggcgatggcg
      661 gcggtcgagg ccgggtactc ggcgggcacg ccacggtggc gaccgtcctc gaaggggacg
      721 ccgtcgaacc agaagaacgg cccgacgctg gcggtcgtgg cccacgtgtc aatggctgct
      781 tcgaggtcgg gaaccagcca gcacacctgc atgaacccgg gtccgggcat cggaacggcg
      841 accatcagcc gatcaccggg aaccgctcgt cgcccagccc ttcccactgg gtcatccgct
      901 tcgtgctgaa gttcgggcac ggcgagccgt tccaacaggc atccgcgggc tgcgtgacga
      961 cgatgtacgc ccaacgaggt cgatcggtga cgttcgcccc ggcgccgtgg accgtgaggt
     1021 gcgtgtgcac cgtgatgtca ccgagctcgt aggtgatcgg atccgagatc ggcagttcac
     1081 ccagctcggg gaagacctcg agcgcgtcgc gatcgccata ggtcgtgtag tcgcccagga
     1141 ccccgtaccg gtgcgaaccg ttgacgaagg acatcgtccc cgcgtcaggg ccgtacgact
     1201 ccagcgggaa ccagaacgtc agcccacctg accggtcgac cgagaaggtg atgaagtcct
     1261 ggtggaacgc cgtgggaccg ttcccccgat gcagcgaccg agaggcgctg ggcagcttcg
     1321 gagcgaagaa gtccgagtag tggcggaccc ccatgggctc gccgggcgcc acgaaccggc
     1381 gctgcagcaa gcgtgcgctc ttgccgacgt cgtccagcag cggtcggagg accgggttcg
     1441 acatgccaaa gctcaggtgg gcgttgaagt agtcgaggcc gaactcgcca tccgccgcgg
     1501 cctgctcgaa ccccgggggg atcgggttgc cgtcgccatc gtcacccatc gcctcgcggg
     1561 cgagctcgag cacccctccg atcacgtccg ggtcgacgaa cgccttcagc tgcacccaac
     1621 cgagctcgtt gagatgttcg atctcctcgg cggtgaccgg acggcacgga gctgcggtta
     1681 cgtcgctcat gcgaagatcc ccggccccgt gcggaacgcg tagaggccga gggcctcgtc
     1741 atcgccaggt gagtacggcg tggccatctc ctcgccggtc ggtccgatct cggcggcgac
     1801 cgcctccagc ttggggcgat cgagtccgta cacgtggatc gccgtgtcgg cgaggtactg
     1861 ccggacgtgc cgttcgtcga cgtcggagaa ggtgaaccgc atcgcctcgc gggtgtgggg
     1921 ccacgtgccc tcggcgtgcg ggtagtcgtc tccccacatg gtgttggcga cgaggtcgtg
     1981 ctcgatcccg agcttcgcct cccagttcga gaggaagctg gcgccgatgt agcagttctc
     2041 ccggaagtac tccgacggtt ggcgaggaag cgtcttgcgc aggtgggcgc cggcgggtcc
     2101 ggtgtagagg ccgtcgaggt ccgcgatgac ctggaccgcc cagtccatcc actgctcggt
     2161 catgaccagc ttcagcccgg ggtgtcgttc gaacacgccg ccgaggatca tcacccagac
     2221 accgcggcgg gtccgccacg tggtctccat catgtacatc gactgcgccc cgtcgccctt
     2281 gtacgggtag tgctcgcccc cgcctccgtg ggtgttgagt gacatgccgg tcgcctcgca
     2341 cgccgaccag agccgctccc acaccggctc gttgagcatg gggaagtcgc cccgcggcgc
     2401 cggaaggttg atgcccttca gacccgcttc ggctgcccac tccacctcgc gcacgcac
//
LOCUS       DATDBZ010000339         6040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1752652, whole genome shotgun sequence.
ACCESSION   DATDBZ010000339 DATDBZ010000000
VERSION     DATDBZ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752652"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VIX62_08195"
     CDS             complement(<1..967)
                     /locus_tag="VIX62_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5860249.1"
                     /translation="MEGRRAVSIKDVARAAGVSTTTVSHALNDKGRLPASTREHVRRV
                     ADDLGYRPSAIARGLVSGRTGVLGLAVSVPGPVSDSFASIQYFAQVINGATAAATARG
                     YALVVVPADGNGEVWRRVAVDGAVVVDPVTGDLNPDAIRGQGLPVVTIASEPDSTEPG
                     WCVDDETDRSIALVMDHLVEQGARHPAVLTWEWHDAFTDGSIDAYRRWCADRSLEPYV
                     VEIETTDLATAFETADRRAAELVDDVAAHDAVFALYEPLGAIVLRAAAERGIAVPAEL
                     LVAAARDTGIGRTTLPTLTVLEFQPERLGAEAVGLLIDRLETPAMD"
     gene            1074..2951
                     /locus_tag="VIX62_08200"
     CDS             1074..2951
                     /locus_tag="VIX62_08200"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY5860250.1"
                     /translation="MTEFRTTRRRFIGMSAAGAAAVLAPGARALAKTATSGPADTVFL
                     GGSVITMDGRRRIAKAMAVSYGRIVYVGGVAGARDWIGARTEVVDLAGGAVMPGIHDG
                     HVHPLYAGRLLQQCSLGYLPLTVDEMRAMIQTCLDDTVDQEPDGWLPVADWDLQSMTP
                     AGLPIVKEILDVLQTQRPIIVRSTDGHNALVNSRALQIAGVTASTPDPIDGTIVRDGQ
                     GNPTGHLIDGAIGLVEQYVPEPTFDQDVASMRTALGVMAARGVTTLNDAITWDYHCSI
                     YEALRSAGELTARVHCDLVLDREPADDMASAIAYFDDVRATYDRRNLTVRTVKIFEDG
                     VMEYPIQTAGLLKPYRVKENGQWVPGPSRGPIYFERPVLKHAVTELDAAGYRVHIHAI
                     GDRAVRVALDGYQAALEANGDSDKRHTIAHLQLVDPADLPRFAELGVLPCMQLQWAER
                     DAYTMEALKRYLGQERWSRIYPSGSLAAAGALVTGGSDWPVDPSGPFDAIQQAVTRQG
                     PFGGKYEPPLNPDQGVPLMDALAMHTNGTAFQLHQERKTGSIEVGKAADVIVLDQDPT
                     AVPIDQVRFTQVQRTFMGGESVFEAGAASKAVERVLKADTAAAAAGVRRRGLASHGGC
                     C"
     gene            complement(2948..3814)
                     /locus_tag="VIX62_08205"
     CDS             complement(2948..3814)
                     /locus_tag="VIX62_08205"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY5860251.1"
                     /translation="MTNRLHGAIAASVTPLTTGGRGVDTSVIGPLVAFLAEGGVDGLL
                     ACGTTGEGVLLTVAERRAVTEAFVASRPTGFQVAIHAGAQTTADTIALAAHAQRTGAD
                     AVAVIAPPYFPLDAEELTRHLVSAANASDPLPFYVYEFAGRSGYAIPPDVVRAVKAQA
                     PNVVGMKVSDAPFEAVAPYLELGLDVFIGNEPLALAGMEGGAVGCVSGLAAAFPEITA
                     ALVHDTSDDAHDQVCLLRDRLAGIPFHAALKEVLAARDVLPTTAVRPPLRALTDTERA
                     TVLAVARELGVG"
     gene            complement(3828..4736)
                     /locus_tag="VIX62_08210"
     CDS             complement(3828..4736)
                     /locus_tag="VIX62_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY5860252.1"
                     /translation="MDPLRKGLLWASENATLREHLPNFRFVRATVDRFMPGESAEDAL
                     AAAQRQFDADAIPVTFTRLGENVQELSQAADAAGDYLRMLDRIEELGLDAEISLKLTQ
                     LGFDLDPDVTATHLARLVDRSAELGRTCWIDMESTHYVAATVDLYEGELSRSPYVGLC
                     LQAYLRRTYEDIQRLLPAEPSIRLVKGAYREPKEVAFQSKASIDESYLRLASHLVKGG
                     ARRVALGSHDTDLIARIDATLGGHDGFEVAMLYGIRTDEQRRLHAEGYPVRTLISYGP
                     SWYPWFMRRIAEKPVENTLLALRNLV"
     gene            complement(4782..6038)
                     /gene="aceA"
                     /locus_tag="VIX62_08215"
     CDS             complement(4782..6038)
                     /gene="aceA"
                     /locus_tag="VIX62_08215"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEY5860253.1"
                     /translation="MQQDWDTNPRWKGIERTYTPADVVALQGSVVEEATLARRGAEVL
                     WNQLHDLEFVNALGALTGGQAVEMIKAGLQAIYLSGWQVAADANVAGQTYPDQSLYPA
                     NSVPQVVRRINNALQRADQIAWASGEGTSTRWMAPILADAEAGFGGSLNAFELMKGMI
                     EAGAAGVHWEDQLASEKKCGHLGGKVLVPTDQFIRTLTAARLAADIADVPTLVVARTD
                     ALAATLLTSDIDERDRKFTNGERTHEGFYQVRNGLDVAIARGQAYAPYADMIWCETAT
                     PDLDEARLFAEGIHAVAPDTMLAYNCSPSFNWKQHLDDATIARFQKELGAMGYKFQFI
                     TLAGFHALNASMFELARGCADEGMTAYVRLQEQEFAMEAEGYTATRHQAEVGTGYFDQ
                     VSTVISGGRASTLALEGSTEEQQFQD"
BASE COUNT          881 a         2078 c         2156 g          925 t
ORIGIN      
        1 cgtccatcgc aggggtttcc agccggtcga tcaagagccc gacggcctcg gctcctagcc
       61 gttcgggctg gaattcgagc accgtcaggg tgggcagcgt cgtccgcccg atccccgtat
      121 cccgtgcggc ggcgacgagc agctcggcgg ggacggcgat cccccgctcg gcggcggcgc
      181 ggaggacgat cgcgcccaga ggttcgtaga gcgcgaagac ggcgtcgtga gcggcgacgt
      241 cgtcgaccag ttcggcggct cgccgatcgg cggtctcgaa cgcggtcgcg aggtcggtcg
      301 tttcgatctc cacgacgtac ggctcgaggg aacggtccgc acaccatcgt cggtaggcgt
      361 cgatgcttcc gtcggtgaag gcgtcgtgcc actcccacgt cagcaccgcg gggtgtcgcg
      421 ctccctgttc caccaggtga tccatcacca aggcgatcga gcggtcggtc tcgtcgtcca
      481 cgcaccatcc cggctcggtg ctgtccggct ccgaggcgat cgtgacgacg ggcaggccct
      541 gaccgcggat cgcgtcgggg ttcaggtccc cggtcaccgg gtcgaccacg acggcgccgt
      601 cgaccgccac gcggcgccat acctcaccgt tgccatcggc cgggaccacc acgagggcat
      661 agccacgagc ggtggccgcc gcggtggcgc cgttgatcac ctgagcgaag tactggatgc
      721 tcgcgaacga gtcggacacc ggtccgggga ccgagaccgc caggccgagc acgccggtgc
      781 gtccggagac cagacctcgc gcgatggcgc tgggccggta gccgaggtcg tcggccaccc
      841 tgcgaacgtg ctcgcgcgtc gacgccggca gccggccctt gtcgttgagg gcgtgcgaga
      901 cggtggtcgt ggacaccccc gccgcacgag cgacgtcctt gatgctgacc gccctgcgac
      961 cttccatccc gcgagcatag cggctcgccg attgaccgcg gttaaacgct tttgctagcc
     1021 tcgcgcctac gtactgcggt cggatcgggc cgcgatggat gaggaggatg ctcgtgaccg
     1081 agttccgaac cacgcgtcgg aggttcatcg ggatgtcggc ggccggggcc gccgcggtgc
     1141 tggcgcccgg cgccagggcg ctggcgaaga ccgcgacgtc ggggccggcg gacacggtct
     1201 tcctcggcgg gtccgtgatc acgatggatg ggcggcgtcg catcgcgaag gccatggcgg
     1261 tctcgtacgg acgcatcgtc tacgtgggcg gcgtcgccgg cgccagggac tggatcggcg
     1321 cccggaccga ggtcgtcgat ctcgccggag gcgccgtgat gccgggcatc cacgatggcc
     1381 acgtccaccc gttgtacgcg ggtcggctgc tgcagcagtg cagcctgggg tacctgccgc
     1441 tcacggtcga cgagatgcgc gccatgatcc agacctgcct cgatgacacc gtcgaccagg
     1501 agcccgacgg ctggttgccg gtggccgact gggacctgca gagcatgacc ccggcgggtt
     1561 tgccgatcgt gaaggagatc ctggacgtgc tgcagacgca gcgcccgatc atcgtccggt
     1621 ccaccgatgg ccacaacgcc ttggtgaact cccgggcgct gcagatcgca ggcgtgacgg
     1681 cgagcacccc tgatccgatc gacggcacga tcgttcgcga cgggcagggc aaccccaccg
     1741 gtcacctgat cgacggggcg atcggcctgg tggagcagta cgtgccggag ccgaccttcg
     1801 accaggacgt cgcctcgatg cgcaccgcgt tgggcgtgat ggcggcgcgg ggggtcacca
     1861 cgttgaacga cgcgatcacg tgggactacc actgttcgat ctacgaggcc ctgcgcagcg
     1921 ccggcgagct cacggcgcgg gtgcactgcg acctcgtcct cgaccgggag cccgccgacg
     1981 acatggcgag cgcgatcgcc tacttcgacg acgtgcgcgc cacctacgat cgccgcaacc
     2041 tcaccgtccg caccgtcaag atcttcgaag acggggtgat ggagtacccg atccagaccg
     2101 cggggctgct caagccctac cgcgtgaagg agaacgggca gtgggtgccc ggcccgagcc
     2161 gcggcccgat ctacttcgag cggcctgtgc tcaagcacgc cgtcaccgaa ctcgacgcgg
     2221 cgggctaccg cgtgcacatc cacgcgatcg gcgaccgtgc ggtgcgggtg gccctcgatg
     2281 gctaccaggc cgcgctcgaa gccaacggcg acagcgacaa gcggcacacg atcgcccacc
     2341 tgcagctggt ggatccggcc gacctgcccc ggttcgcgga gctgggcgtg ctgccgtgca
     2401 tgcagctgca gtgggccgag cgcgacgcct acacgatgga ggcgctcaag cgatacctgg
     2461 gccaggagcg ctggtctcgg atctacccgt cgggcagcct ggccgccgcc ggtgcgctgg
     2521 tgaccggcgg gagcgactgg ccggtggatc cgagcggacc gttcgatgcg atccagcagg
     2581 cggtgacccg ccagggtccg ttcggcggca agtacgaacc gccgctcaac cccgaccagg
     2641 gcgtgcccct gatggatgcc ctcgcgatgc acacgaacgg cacggcgttc cagttgcacc
     2701 aggagcgtaa gaccggctcg atcgaggtgg gcaaggccgc cgacgtgatc gtgctcgatc
     2761 aggaccccac ggccgtgccg atcgatcagg tgcggttcac ccaggtgcag cgcacgttca
     2821 tgggcggcga gtcggtgttc gaggccggtg ccgcgtccaa ggcggtcgaa cgggtgttga
     2881 aggccgacac ggcggccgcc gcagcgggcg tgcgccggcg cggtctcgct tcgcacggag
     2941 gctgctgcta gcccacgccg agctcgcggg ccacggccag gaccgtggcc cgctcggtgt
     3001 cggtgagtgc tcgcagcggt ggtcgcacgg cggtggtggg tagcacgtcg cgtgccgcca
     3061 gcacctcctt gagtgcggca tggaagggga taccggccag ccggtcacgc agcaggcaca
     3121 cctggtcgtg ggcgtcgtcg ctcgtgtcat gcacgagcgc cgcggtgatc tccgggaacg
     3181 cggcggccag tccgctcacg cacccgacgg cgccgccctc catccccgcc agcgccaatg
     3241 gctcgttgcc gatgaacacg tccaggccga gttccaggta cggcgcaacc gcctcgaacg
     3301 gggcgtcgga caccttcatc ccgacgacgt tgggagcctg ggccttgacc gctcgaacga
     3361 catcaggggg gatcgcgtaa ccgctccgtc ccgcgaactc gtacacgtag aacggcaggg
     3421 ggtcgctcgc gttcgccgcg ctgaccaggt gacgggtgag ttcctcggcg tccagcggga
     3481 agtacggcgg cgcgatcacg gcgacggcgt cggcgcccgt ccgctgggcg tgcgccgcca
     3541 gggcgatcgt gtcggccgtg gtctgggcgc cggcgtggat cgccacctgg aagccggttg
     3601 gccgcgacgc gacgaaggcc tcggtcaccg cccggcgttc ggcgacggtc aacagcacgc
     3661 cctccccggt ggtcccgcac gcgaggaggc cgtcgacgcc gccctcggcc aggaacgcca
     3721 cgagcggccc gatcaccgac gtgtccacgc ctcggccgcc ggtggtcaac ggggtcaccg
     3781 aagcagcgat cgcgccgtgc agtcggttcg tcacgcctgc ctcccggtca tacgaggttc
     3841 cgcagggcga gcagggtgtt ctccaccggc ttctcggcga tcctgcgcat gaaccacggg
     3901 taccacgacg ggccgtagga gatcagcgtg cgcaccgggt agccctcggc gtggagccgc
     3961 cgctgttcgt cggtgcggat gccgtacagc atcgccactt cgaagccgtc atggccgccg
     4021 agggtggcgt cgatccgcgc gatcaggtcg gtgtcgtgcg agcccagcgc gacccgcctc
     4081 gcgccgccct tcaccaggtg gctcgccaga cggaggtagc tctcgtcgat cgacgccttg
     4141 ctctggaagg cgacctcctt cggttctcgg taggcgccct tcacgagccg gatcgacggc
     4201 tcggccggca gcagccgctg gatgtcctcg taggtgcgcc ggaggtaggc ctggaggcag
     4261 agccccacgt agggcgagcg cgagagttcc ccctcgtaga gatcgacggt ggccgcgacg
     4321 tagtgggtgg actccatgtc gatccaacac gtgcgcccca gctcggccga gcggtcgacg
     4381 agccgcgcga gatgggtggc cgtcacatcc gggtccaggt cgaagcccag ctgcgtgagc
     4441 ttcagcgaga tctcggcgtc caggccgagc tcctcgatcc ggtcgagcat gcggaggtag
     4501 tcgcctgccg cgtcggccgc ctgcgacagc tcctggacgt tctcgcccag ccgggtgaac
     4561 gtcacgggga tggcgtcagc gtcgaactga cgctgcgccg cggcgagcgc gtcctcggcg
     4621 ctctccccgg gcatgaaccg gtcgacggtg gcccgcacga accggaagtt gggcaggtgc
     4681 tcccggagcg tggcgttctc cgaggcccac agcagccctt tgcgcagcgg atccatcgcg
     4741 ggtcacgcta ccccagcgcg ggccggagta tccgtccgac gtcagtcctg gaactgctgc
     4801 tcctcggtgg acccttccag ggccagggtc gaggcccggc caccgctgat cacggtcgag
     4861 acctggtcga aatacccggt tccgacctcg gcctggtggc gcgtggcggt gtagccctcg
     4921 gcctccatcg cgaactcctg ctcctggagc cgcacgtagg cggtcatgcc ctcgtcggcg
     4981 cagccgcgcg cgagttcgaa catgctggcg ttgagcgcgt ggaacccggc gagcgtgatg
     5041 aattggaact tgtagcccat ggcgcccagt tccttctgga agcgggcgat cgtggcgtcg
     5101 tccaggtgct gcttccagtt gaacgacggg ctgcagttgt aggcgagcat cgtgtcgggg
     5161 gccacggcgt ggataccttc ggcgaacagc cgagcctcgt ccaggtcggg cgtcgcggtc
     5221 tcgcaccaga tcatgtcggc gtacggcgcg taggcctgcc cgcgcgcgat cgcgacgtcg
     5281 agcccgttgc gcacctggta gaagccttcg tgcgtgcgct cgccgttcgt gaacttccgg
     5341 tcgcgctcgt cgatgtcgga ggtgagcagc gtggccgcca gcgcgtcggt gcgcgccacc
     5401 accagggtgg ggacgtcggc gatatcggcg gcgaggcgag ccgcagtcag cgtccggatg
     5461 aactggtcgg tgggcaccag caccttgccg cccaggtggc cgcacttctt ctcgctggcc
     5521 agctggtcct cccagtggac gcccgcggcg ccggcttcga tcatgccctt catcagctcg
     5581 aacgcgttga gggaaccacc gaagccggcc tcggcgtcgg cgaggatcgg tgccatccat
     5641 cgggtgctcg tcccttcgcc ggaggcccag gcgatctggt cggcacgctg caacgcgttg
     5701 ttgatgcggc gcaccacctg cggcacggag ttggcgggat agagcgactg gtcggggtag
     5761 gtctgacccg cgacgttggc gtcggccgcc acctgccagc cggacaggta gatcgcctgc
     5821 aatccggcct tgatcatctc gacggcctgg ccgccggtca gggcgcccag cgcgttgacg
     5881 aactccaggt cgtgcagctg gttccacagc acctcggcgc cgcggcgggc cagcgtggcc
     5941 tcctcgacga ccgaaccctg cagtgccacg acgtcggccg gggtgtaggt gcgctcgatg
     6001 cccttccacc gcgggttggt gtcccagtcc tgctggattt
//
LOCUS       DATDBZ010000340         3271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1958674, whole genome shotgun sequence.
ACCESSION   DATDBZ010000340 DATDBZ010000000
VERSION     DATDBZ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958674"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VIX62_08220"
     CDS             <1..1190
                     /locus_tag="VIX62_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860254.1"
                     /translation="APLWLMRLALVLGSSLLAALVFRLWVPIVGWGAPMGMVLFMGSW
                     PALFYGSEVMPNLWAALFAVGALAFVGRCATGPDVRPWDLAGIGLMAAAMALMRPPDA
                     VVLAVAVSVVVLAMRGSVRSIVVVIVGVSVGTLPWLVEMSVRYGGPREAIEAARTVSH
                     AGGAFSGVAEHLRLTDGPLLGPEPGGALPWVGGLWWVVLVGLSAWALRPGADRRRRVG
                     VRMAAVVGVALAAEYLFLVAGLAPRFLLPALAVLSVSAGGGLEALRGQGHLGTNACVV
                     VAMILAGWLLWQVGTFDRLEALARDQRRIPEAVGSAVGDAIGEQGCVVASTDDFAQVA
                     FAAGCAGRDLTDLASTTIDRVLEAADGRRVVVVSRSGGVTELPGTPIALPDAAWTAVA
                     VTP"
     gene            1187..2302
                     /locus_tag="VIX62_08225"
     CDS             1187..2302
                     /locus_tag="VIX62_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEY5860255.1"
                     /translation="MTRDVLSVRALNRATLARQLLLERASVSPIEAVHHLVGLQAQIP
                     NNPYIGLWSRLAGFDPMSVSALVRDRALVRLTVMRGTLHLVTADDCLLLRPLVQPVLD
                     DERRHHRDFAPLLEGVDLEPVIAFGREVLSERASSGTELRAAMQDRFPDLDAGALAFA
                     CRSGLALVQVPPRGEWGRSSQVRLMTVEAFLGRPVESQPSVDEVVLRYLGAFGPATTS
                     DISAWCRLRGMAEVVERLRPRLRVFRSERGRELFDLPEAPRPDPGVPAPIRFLPEYDN
                     LWLAHDDRSRVLTEDDGRWMQLASAPFRGSVLIDGWLRASWHVEGPGKDGRAALHVTP
                     TGRLPKRTVASVSAEARRLLRMIGNPDDDVSVAEPPD"
     gene            complement(2314..>3271)
                     /locus_tag="VIX62_08230"
     CDS             complement(2314..>3271)
                     /locus_tag="VIX62_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860256.1"
                     /translation="AIMAVNGTTFNNSFTVYGSSGNNGDIYILNGNLNISNSPTVYGN
                     VYVPLGSATLGNNSEVKGDVWANGTVSVSTPSVVSGNAKSSAGNISGSGTVTGDATAL
                     GTTTIGTVGGSRYPGTNPGPVPTQTFPQITNSTTAWTNAGYTLVGPFTGGSACTNAYN
                     YIHNTGGGTWATSGLTNIVVQISATCTFQNGNNDTNTIRGNLAVISDGGFTFSQMSNW
                     KGVSAPVKNVYFISGYTAAACTGTKNITVGNNTNFDAFTNVFFYTPCTANMNNTNNFN
                     GQVLAANVTIGNQFTMTFQPVLVPAQGTVTGFTQDVAFIREV"
BASE COUNT          399 a         1058 c         1191 g          623 t
ORIGIN      
        1 gggccccgct gtggctcatg cgtttggccc tcgtcctggg aagctcgctc ctggcggccc
       61 tggtcttccg cctctgggtc ccgatcgtgg gatggggcgc ccccatgggg atggtgctgt
      121 tcatggggtc gtggccggcg ctgttctacg gatcggaggt gatgccgaac ctgtgggcgg
      181 cactgttcgc ggtgggagcg ctggccttcg tcggccggtg tgcgaccggc ccggatgtcc
      241 gaccttggga tctggcgggc atcggactca tggccgcggc catggcgctg atgcgaccac
      301 cggatgccgt ggtcctcgcg gtggcagtct cggtcgtggt cctggcgatg cgcggatcgg
      361 tgcggtcgat cgtggtcgtg atcgtggggg tatcggtcgg cacgctcccc tggttggtcg
      421 agatgagcgt tcgatacggc gggcctcgcg aagccatcga ggccgcgcgc acggtgtccc
      481 acgcgggcgg cgcgttctcg ggcgtcgccg aacacctgcg gctcaccgac ggcccgctgc
      541 tcggccccga gccgggtggc gcgctcccct gggtgggcgg cctgtggtgg gtggtgctgg
      601 tcggtctcag cgcgtgggcg cttcggcccg gcgcggatcg ccggcgaagg gtgggggtgc
      661 gcatggctgc cgtcgtgggg gtcgccctcg cggcggagta tctgttcctc gtcgcgggcc
      721 tcgcgccgcg gttcctgttg ccggcgctcg cggtgctttc cgtttcggcg ggtggaggcc
      781 tggaggcgct ccgcggtcaa gggcacctcg gaacgaacgc atgcgtggtg gttgcgatga
      841 tcctcgcggg ttggctcctg tggcaggtcg gcaccttcga ccggttggag gccctggcac
      901 gtgaccagcg tcggatccct gaggccgtgg gatccgcagt cggtgacgcc atcggcgaac
      961 agggatgcgt ggtcgcgagc acggacgact tcgcgcaggt ggcgttcgcg gcagggtgcg
     1021 ccgggaggga cctgaccgat ctcgcgtcga ccacgatcga ccgcgtcctc gaggccgccg
     1081 acggtcgccg ggtcgtggtc gtgagcagga gtggcggtgt caccgagcta cccgggacac
     1141 cgatcgccct ccccgatgcc gcctggaccg cggtggcggt gacaccgtga cccgggacgt
     1201 gctctcggtg cgggcgctga accgggcaac gctcgctcgg caactgctgc tggaacgcgc
     1261 gtccgtctcc ccgatcgagg ccgtgcacca cctcgtgggt ctgcaggcgc agatcccgaa
     1321 caacccgtac atcggcctgt ggtcgcggct cgccggcttc gacccgatgt cggtatcggc
     1381 tctggtccgc gatcgcgcgc tcgtgcgact cacggtgatg cgcgggacgc tgcatctcgt
     1441 cacggcggac gactgtctgc tgctgcggcc actcgtgcaa cccgtgctgg acgacgaacg
     1501 acggcaccat cgcgacttcg ccccgctcct cgagggcgtc gacctcgagc cggtgatcgc
     1561 gttcggccgc gaggtgctgt cggagcgtgc atcgagcggt acggaactcc gcgccgcgat
     1621 gcaggaccgg ttccctgatc tcgatgccgg ggcgctcgcg ttcgcctgcc ggagcggtct
     1681 cgccttggtc caggtgccgc cgcgaggcga atgggggcgc tcctcacagg tccggctgat
     1741 gacggtcgag gcgttcctcg ggcggccggt cgagtcgcag ccgtccgtcg acgaggtggt
     1801 cctgcgctac ctcggcgcgt tcggacctgc gaccacgagc gacatctcgg cgtggtgccg
     1861 gctccgaggg atggccgagg tggtcgaacg gctgcgtccc cgactccgtg tgttccgcag
     1921 cgagcgcggt cgggagctct tcgacctccc tgaggcgccc cggcccgatc ctggcgtgcc
     1981 ggcgccgatc cgcttcctgc cggaatacga caacctctgg ctcgctcacg acgaccggtc
     2041 gcgtgtgctc acggaggacg atggtcgctg gatgcagctc gcgagcgccc cgttccgcgg
     2101 ctcggtgttg atcgacgggt ggctccgcgc gtcgtggcac gtggaaggcc cgggcaagga
     2161 cgggagggca gccctgcatg tcacgccgac cggccggctt ccgaagcgga cggtcgcgtc
     2221 ggtgtccgca gaggcccgtc gactgcttcg gatgatcggc aacccggacg acgacgtcag
     2281 cgtggcggag ccgcccgact gaacggcgtg gcgtcagacc tcgcggatga acgcgacgtc
     2341 ctgggtgaac ccggtcaccg ttccctgggc gggtaccagc accggctgga acgtcatcgt
     2401 gaactggttg ccgatcgtga cgttcgccgc gagcacctga ccgttgaagt tgttggtgtt
     2461 gttcatgttc gcggtgcacg gcgtgtagaa gaacacgttc gtgaacgcgt cgaagttcgt
     2521 gttgttgccc acggtgatgt tcttcgtgcc ggtgcaggcg gctgccgtgt atccactgat
     2581 gaagtacacg ttcttgaccg gagccgacac gcccttccag ttggacatct ggctgaacgt
     2641 gaacccgccg tcgctgatca ccgcgaggtt gcctcggatc gtgttcgtgt cgttgttgcc
     2701 gttctggaac gtgcacgtgg cactgatctg gaccacgatg ttggtgagcc cgctcgttgc
     2761 ccacgtccca cccccggtgt tgtggatgta gttgtacgcg ttcgtgcaag ctgacccgcc
     2821 ggtgaacggt cccaccaggg tgtatcccgc gttcgtccag gccgtcgtgg agttcgtgat
     2881 ctgcgggaat gtctgcgttg gcaccggtcc ggggttcgtg ccggggtagc gcgaaccacc
     2941 gaccgtgccg atcgtggtgg tccccagcgc ggtggcatcg cccgtgaccg tgccgctccc
     3001 cgagatgttt cccgcgctgg acttcgcgtt cccgctcacc accgacgggg tgctgaccga
     3061 gacggtgccg ttggcccaca catcgccctt gacctccgag ttattcccga gggtcgccga
     3121 ccccaacggc acgtagacgt tgccgtagac cgtcggtgag ttcgagatgt tcaggttccc
     3181 gttcaggatg tagatgtcgc cgttgttgcc gctgctcccg tacacggtga agctgttgtt
     3241 gaacgtcgtg ccgttcacgg ccatgatcgc g
//
LOCUS       DATDBZ010000341         4382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_94702,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000341 DATDBZ010000000
VERSION     DATDBZ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94702"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VIX62_08235"
     CDS             complement(<1..695)
                     /locus_tag="VIX62_08235"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5860257.1"
                     /translation="MAQATGSSKPDTIVMVHGLWMTGRSWEHWKERYEARGYTVHTPG
                     WPGFDVEVEALNADPSPMNDLHADEVIDSYEAFIKGLPTSPIIMGHSLGGAVTQVLLN
                     RGLGCVGVGVSAATVKGVRDLPFSTLKAAAPALNPFKRGKPVPLTEKEFHYAFANTTT
                     IEESNEWYKRYCVPAATGVLADIALANLHRNPPTEVDFAKEGRIPLLFIGFSEDHVVP
                     PKATMHNERKYDDS"
     gene            859..1254
                     /locus_tag="VIX62_08240"
     CDS             859..1254
                     /locus_tag="VIX62_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5860258.1"
                     /translation="MLVTTEDQLDAVFGALADPTRRSILARLTQGEATVAELVAPFEM
                     SQPAISRHLKVLERAGLITRTRRATARLSHLRAKPLQDATEWLTAYREFWEERYERLD
                     EVLAALQEHDTDRSPDPDRGSDPTGRSTS"
     gene            1251..1751
                     /locus_tag="VIX62_08245"
     CDS             1251..1751
                     /locus_tag="VIX62_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5860259.1"
                     /translation="MTELKVTAEPGVPQILTEREFDAPRELILRAFTEADLLAQWLGP
                     RGYTMTIERFEPRAGGSWRYVHSDGDGNSYGFHGVFHNDPSPDGFVQTFEFEGAPGHV
                     SLDTMVLEERDGRTIVRTNSLFQSVEARDAMVQSGMADGMSQGYDQLEELLEKQGGSL
                     KARHDS"
     gene            1794..2267
                     /locus_tag="VIX62_08250"
     CDS             1794..2267
                     /locus_tag="VIX62_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5860260.1"
                     /translation="MQKITPCLWFDTQAEEAANFYVSLFDDAEVTRVERLDGTPSGDN
                     VALVFFRLAGQEYMAINGGPQFPFTEAVSFHVSCKDQDEVDRFWAQLTADGGEEGQCG
                     WLKDRFGLSWQIVPERMGELLSDPNPARSQAAMQAMLQMQKIEVKVLEEAADAAA"
     gene            2291..2704
                     /locus_tag="VIX62_08255"
     CDS             2291..2704
                     /locus_tag="VIX62_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5860261.1"
                     /translation="MDYKLELLPVPVTDVDRAKAFYADQLGFVVDVDDSPMESIRVVQ
                     LTPPGSACSIMVGTGITRSEPGSLQGPQLVVDDIEAAREELAGRGVDVSPVQHFTENG
                     LADGKGGPWNSWVFFSDPDGNGWVLQERPAATDRT"
     gene            complement(2709..>4382)
                     /locus_tag="VIX62_08260"
     CDS             complement(2709..>4382)
                     /locus_tag="VIX62_08260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5860262.1"
                     /translation="FLSDRITSGHQLPYTMPAGGGEPTLAATLTGSAENVAWSNDGNR
                     LLVLAADPGSYGLDWGARAVRGAEPESDPVVRRPGDARRRLFLIDLGSGNATEVGPPD
                     LSIWEVDWDGDHTAVAVVATDHTGSGWYQGVVARLDLQARTAHTLYEPEWQMEGLERS
                     PDGTRALIVEGYASDHGLLAGSIMVIDVATGETSDPWPDLQSVGLASWCDDDSIWYAS
                     TEGTGNACGRIWLDGRQEERWRDDAFIGDAVTTPACVISEGAADVWTTHQAHATPPEL
                     AWFDHDSGSWVRRTSFNDHIVRGRVFPDARTVRWMGEGGVEIEGVLMTPHGAEGPLPM
                     IVGVHGGPTWNWGSYFSDSEPNAVLLASAGYACLLPNPRGSIGRGHVFNQGVIGDGGG
                     IDFRDIMAGVDYVIAEGIADPDRLGIAGLSYGGYMAAWAVGQTDRFGASVAMSVVSNY
                     LSFHLTSEVWWYDHAILEGDWHDPASQYMDRSPVTHAHKGSTPTLILQGAIDRCTPVS
                     QADELFTAIAETGTEVELVVYPREGHVPLERGHALDAIRRTQEWFDRHL"
BASE COUNT          722 a         1486 c         1477 g          697 t
ORIGIN      
        1 gagtcgtcgt acttccgctc gttgtgcatc gtggccttgg gcggcaccac gtggtcctcg
       61 ctgaatccga tgaacaacaa cgggatgcgg ccttcctttg cgaagtcgac ctcggttggc
      121 gggttccggt gcaggttggc cagcgcgatg tcggcgagca cgcccgtggc cgccgggacg
      181 cagtagcgct tgtaccactc gttcgactct tcgatcgtgg tggtgttggc gaacgcgtag
      241 tggaactcct tctcggtgag cggcaccggc ttgcctcgct tgaacgggtt gagcgccggc
      301 gcggcggcct tcaacgtgga gaacgggaga tcgcgaacgc ctttcaccgt ggcagccgag
      361 acgccgacgc cgacgcagcc caggccacgg ttcaacagga cctgggtcac ggcgccaccg
      421 agcgagtggc ccatgatgat gggcgaggtc ggcaagcctt tgatgaacgc ctcgtacgaa
      481 tcgatcacct cgtcggcgtg gagatcgttc atcggcgacg ggtcggcgtt caacgcctcg
      541 acctcgacgt cgaacccggg ccagccgggg gtgtggaccg tgtaaccgcg cgcctcgtag
      601 cgctccttcc agtgctccca actccggccg gtcatccaca gtccgtggac catcacgatg
      661 gtgtcgggtt tgctgctccc cgtggcctgc gccatacggt tccccttcct gcagctgaga
      721 cctccaacct aacgcgttgc cgcgattccg tgcggggggg ttgcatgacg actcggtgaa
      781 cggaacgaag cgaagcacct tgacggtgaa ccctctcgtc atgtataacg gtctcgttct
      841 ataagggaga cgttatccat gttggtgacg acggaggatc agctcgatgc ggtgttcggg
      901 gcgctggccg accccacgcg tcggtcgatc ctggcccgcc tcacgcaggg cgaggccacc
      961 gtcgccgaac tggtggcgcc gttcgagatg tcgcagcccg cgatctcccg ccacctcaag
     1021 gtgctcgagc gcgcgggcct catcacccgg acccggcgcg ccaccgcccg cctgagtcac
     1081 ctgcgggcca agcccttgca ggacgccacc gaatggctga ctgcctaccg cgagttctgg
     1141 gaagaacgct acgagcggct cgacgaggtc ctcgcggcgc tgcaggagca cgacaccgac
     1201 cgatcgcccg accccgaccg ggggagcgat ccgacaggaa ggagcacgtc atgaccgaac
     1261 tcaaggtcac cgccgaaccc ggcgttccgc agatcctcac cgagcgggag ttcgatgcac
     1321 cgcgcgaact gatcctccgg gcgttcacgg aggccgacct gctggcgcag tggctgggac
     1381 cgcgcggcta cacgatgacg atcgagcggt tcgagccgcg tgccggcggc agttggcgct
     1441 acgtccacag cgatggtgac ggcaactcct acgggttcca cggggtgttc cacaacgacc
     1501 cttcgcccga cggcttcgtg cagacgttcg agttcgaggg cgcacccggc cacgtctcgc
     1561 tggacaccat ggtgctcgaa gagcgcgacg gccggacgat cgtccgcacg aactcgctgt
     1621 tccagtcggt cgaagcccgc gacgcgatgg tgcagtcggg gatggccgac ggcatgtccc
     1681 agggctacga ccagctggag gaactcctcg agaagcaggg tgggtccctg aaggctcgcc
     1741 acgattcgta gcggagcgcc ggatcgcttc gcaccgacag aaaggtggat ccgatgcaga
     1801 agatcacccc atgcctgtgg ttcgacaccc aggcggagga agccgccaac ttctacgtgt
     1861 cgctcttcga cgatgccgag gtgacgcggg tggagcgcct cgacggcacc cccagcggcg
     1921 acaacgtggc gctggtgttc ttccggctcg ccggtcagga gtacatggcc atcaacggcg
     1981 ggccgcagtt cccgttcacc gaagcggtgt cgttccacgt gagctgcaag gaccaggacg
     2041 aggtcgaccg cttctgggcc cagctcaccg ccgacggcgg agaagagggt caatgcggat
     2101 ggctcaagga ccggttcggt ctgagctggc agatcgtccc cgagcggatg ggcgagttgc
     2161 tgagcgaccc caaccccgct cgctcgcaag cggcgatgca ggccatgctg cagatgcaga
     2221 agatcgaggt caaagtgttg gaggaggcgg ccgacgccgc agcctgaccg acgagaagga
     2281 gacgcatcgc atggactaca agctcgagtt gctccccgtg cccgtgaccg acgtggaccg
     2341 cgccaaggcg ttctacgccg accagctcgg cttcgtcgtc gatgtcgacg actcgccgat
     2401 ggagagcatc cgggtggtcc agctgacacc gccgggttcg gcctgctcga tcatggtggg
     2461 cacggggatc accaggtccg aacccggttc gctccagggg ccgcagctgg tcgtggacga
     2521 catcgaagcc gctcgcgagg aactcgccgg ccggggcgtc gacgtgagcc cggtccagca
     2581 cttcacggag aacgggctcg ccgatggcaa gggtggcccg tggaactcgt gggtgttctt
     2641 cagtgacccc gacggcaacg gctgggtcct gcaggaacgc ccggctgcca cggatcggac
     2701 ctgatcgact acaggtggcg gtcgaaccac tcctgcgttc gccggatcgc atcgagcgcg
     2761 tggccgcgtt cgagcggcac gtggccttcg cgcgggtaga ccaccagttc gacctcggtg
     2821 ccggtctcgg cgatcgcggt gaacagctcg tcggcctggc tcaccggggt gcaccggtcg
     2881 atcgcaccct gcaggatcag cgtgggtgtc gaccccttgt gtgcgtgcgt caccggtgag
     2941 cggtccatgt actggctcgc cgggtcgtgc cagtcgccct cgaggatcgc gtggtcgtac
     3001 caccacacct ccgaggtgag gtggaacgag aggtagttcg acaccaccga catcgcgacg
     3061 ctcgcgccga accggtcggt ctggccaacc gcccacgccg ccatgtaccc gccgtaggac
     3121 aagcccgcga tacccaaccg gtcggggtcg gcgatgccct cggcgatcac atagtcgacc
     3181 ccggccatga tgtcgcggaa gtcgatcccg ccgccgtcgc cgatcacacc ctggttgaag
     3241 acgtggccgc gcccgatgct tccgcgcggg ttcggcagca gacacgcgta gcccgccgag
     3301 gcaagcagca ccgcgttcgg ttcggaatcg gagaagtagg acccccagtt ccacgtggga
     3361 ccgccgtgga cgccgacgat catcggcagc ggcccctccg cgccgtgcgg cgtcatcaac
     3421 acgccctcga tctcgacgcc gccctcgccc atccaccgga ccgtgcgtgc gtcggggaac
     3481 acgcgcccgc gcacgatgtg gtcgttgaac gaggtgcggc gcacccagga accggaatcg
     3541 tggtcgaacc atgcgagctc gggcggcgtg gcgtgcgcct ggtgggtggt ccacacgtcg
     3601 gcggcacctt cgctgatcac gcaggccggg gtggtgacgg cgtcgccgat gaacgcgtca
     3661 tcgcgccagc gttcctcctg ccggccgtcc agccagatgc ggccgcaggc gttgcccgtg
     3721 ccttcggtgc tcgcgtacca gatcgaatcg tcgtcgcacc acgacgccag gcccaccgac
     3781 tgcaggtcgg gccagggatc ggaggtctcg ccggtggcga cgtcgatcac catgatgctg
     3841 ccggcgagca gcccatggtc gctggcgtat ccctccacga tcagcgcacg ggtgccgtcg
     3901 ggcgagcgtt ccaacccctc catctgccac tcgggctcgt agagcgtgtg tgcggtgcgc
     3961 gcctgcagat cgagccgcgc caccacgccc tggtaccagc ccgatcccgt gtgatccgtg
     4021 gcgacgaccg ccaccgcggt gtgatcgccg tcccagtcca cctcccagat gctgaggtcg
     4081 ggcggaccca cctccgtggc gtttccggat ccgaggtcga tcaggaacag tcggcgccgc
     4141 gcatcgcccg gccgccgcac gacgggatcg gactcaggct cggcgccacg gaccgcgcgc
     4201 gctccccagt ccagcccgta ggaaccggga tcggcggcga gcaccaggag acggttccca
     4261 tcgttcgacc acgcgacgtt ctcggccgaa ccggtcagcg tggccgccag cgtcggttcg
     4321 ccaccaccgg ccggcatcgt gtacggcagt tgatgcccgg acgtgatgcg atcggacagg
     4381 aa
//
LOCUS       DATDBZ010000342         2735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1122743, whole genome shotgun sequence.
ACCESSION   DATDBZ010000342 DATDBZ010000000
VERSION     DATDBZ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122743"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VIX62_08265"
     CDS             <1..302
                     /locus_tag="VIX62_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017788200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5860263.1"
                     /translation="YLEASSDLGAKGWTTFRRVIWPLALPGIVAGSIFSFSLTLGDYI
                     APILVGNTKFIGNVIYDNVGVANNIPFAAAFALVPVAIMAVYLWIARKLGAFDAL"
     gene            330..1142
                     /locus_tag="VIX62_08270"
     CDS             330..1142
                     /locus_tag="VIX62_08270"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860264.1"
                     /translation="MVRFGTVITLLFLYIPLVVVVIYAFNDSVAQVWPPKGFTTKWFS
                     LAFGDANMRAAFLTSIKVASITTLIALGLGSAAAFAVHRFRFFGRQAISFVLVLPIAL
                     PGIITGMALRTGINTAGINFSVWTIMIGHATFCIVVVFNNVVARLRRTPTSLMEASMD
                     LGADGWQTFRRITLPAIATALVAGGLLAFALSFDEIIVTNFVSGAEITLPKWIFNAIR
                     LPRNRPEANAVAVLVMLLSLVPVYLAQRLAGGGGSASDRGAVGVRTVEVPGE"
     gene            complement(1149..1685)
                     /locus_tag="VIX62_08275"
     CDS             complement(1149..1685)
                     /locus_tag="VIX62_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEY5860265.1"
                     /translation="MSMDPDAPKSQVVEAVPVEPGTDAGTAAGEDTPGDRNESITQPA
                     KLLRIASMVRELLDETRQASLDEAGRSRLADIYHRAVGELSEVLSPDLQEELADLAPP
                     LAGVPSESEIRVAQAQLVGWLEGLFHGIQAAMFAQQAAARQQFEELRRRGLPGQDQGM
                     QGPDGPPQDGSVRGGQYL"
     gene            complement(1729..>2735)
                     /locus_tag="VIX62_08280"
     CDS             complement(1729..>2735)
                     /locus_tag="VIX62_08280"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5860266.1"
                     /translation="GAADDKAGIAIHAAALRALGVHEGATLPVTVTILVEGEEECSTE
                     HLPELVGGQGDLLRADVAIVADGGNYRTGVPTIGTSVRGVTDCVVTVRVLPIAQHSGA
                     YGGPIPDAITALSRVIATLHDEHGDVAIEGLHTFTWTGMQVPEDELREESRVLDSVQL
                     LGTGTIADRLLSKPAVAVLGFDAPRIEGSSNQLVPEASARVSLRLAPGDDPERGRDAL
                     VAHLLDAAPWGVEVEVEAAEAGMGYLVDTTTPAYAAAKDALADAFGREVVEIGSGGSI
                     PLVPLLAETFPGMSVLIVGAGDERSNYHSLDESVDLDDLASMALAEALLIGNLGTPSV
                     "
BASE COUNT          383 a          947 c          935 g          470 t
ORIGIN      
        1 cctacctaga ggcctcgagc gacctggggg cgaaggggtg gacgacgttc cgccgggtga
       61 tctggccact ggcgttgccg ggcatcgtgg caggttcgat cttctcgttc tccctcacgc
      121 tgggggacta catcgcgccc atcctcgtgg ggaacaccaa gttcatcggc aacgtgatct
      181 acgacaacgt cggcgtggcg aacaacatcc cgttcgccgc cgcgttcgcg ttggtgccgg
      241 tggcgatcat ggccgtctat ctctggatcg cgcgcaagct cggcgcgttc gacgccctct
      301 agcccatgga atcccgaggc gctcgtttca tggtccggtt cggcaccgtg atcacactgc
      361 tgttcctcta catcccgctc gtagtggtcg tgatctatgc gttcaatgac agcgtggcgc
      421 aggtgtggcc gcccaagggc ttcaccacga aatggttctc gctggcgttc ggtgacgcca
      481 acatgcgggc ggcgttcctg acttcgatca aggtggcctc gatcacgacc ctgatcgcgc
      541 tcgggctcgg gagcgccgcg gcgttcgcgg tgcaccgctt ccggttcttc ggccgccagg
      601 cgatctcgtt cgttctggtg cttccgatcg cactgcccgg gatcatcacc gggatggcgt
      661 tgcggaccgg catcaacacc gcagggatca acttcagcgt gtggacgatc atgatcggtc
      721 acgccacatt ctgcatcgtg gtggtgttca acaatgtggt ggcacgactc cgaagaacgc
      781 cgacctcctt gatggaagca agcatggacc tgggtgcgga cggatggcag accttccgcc
      841 ggatcaccct gcccgcgatc gcgacggcgc tcgtcgccgg cgggctgctc gctttcgcac
      901 tgtcgttcga cgagatcatc gtcacgaact tcgtctcggg cgccgagatc accctgccga
      961 agtggatctt caacgcgatc cggctcccca ggaaccgtcc tgaggccaac gcggtggcgg
     1021 tcctggtgat gctcctgtcg ctggtcccgg tctacctggc gcagcggttg gcgggtggcg
     1081 gaggctccgc gagcgaccgc ggtgcggtag gcgtgcggac cgtcgaggtg ccgggagagt
     1141 agcgctcctt acaggtactg gccgccgcgc accgaaccgt cctgtggcgg gccgtcgggt
     1201 ccctgcatgc cctggtcctg gccggggagg ccgcggcggc gcagttcctc gaactgctgc
     1261 cgcgccgccg cctgctgggc gaacatcgcg gcctggatcc cgtggaacag gccttcgagc
     1321 cagcccacca actgcgcctg cgccacgcgg atctcggatt cgctcggcac cccggcgagc
     1381 ggcggcgcca ggtcggccag ctcctcctgc aggtcgggtg acagcacctc ggacagctcg
     1441 ccgacggcac ggtggtagat gtcggccagg cgcgaccggc ccgcttcgtc caacgaggcc
     1501 tggcgcgtct cgtccagcag ttcacgcacc atgctcgcga tccgcaacag cttggcgggc
     1561 tgcgtgatcg attcgttccg gtcgcccggg gtgtcctcgc ccgcggcggt cccggcgtcc
     1621 gtgccgggtt ccaccggcac ggcttccacg acctgactct tgggtgcgtc ggggtccatg
     1681 ctcatggctt caacctagca ccggcggacg aacgtgccgc ccggtggatc agaccgacgg
     1741 ggtcccgagg ttgccgatca gcagcgcctc ggccagggcc atcgacgcga gatcgtccag
     1801 gtcgacgctc tcgtccagcg agtggtagtt gctgcgctcg tcgccggcgc ccacgatcag
     1861 gaccgacatg cccgggaacg tctcggccag caatggcacc agggggatcg acccgccgct
     1921 gccgatctcc acgacctcgc gaccgaaggc atcggcgagc gcgtccttgg cggcggcata
     1981 ggcgggcgtg gtggtgtcca ccaggtagcc catccccgcc tcggcggcct cgacctccac
     2041 ctccacgccc cagggagcgg catcgagcag gtgtgcgacg agcgcgtcgc gaccgcgctc
     2101 ggggtcgtcc ccgggcgcga gccgcagcga gacgcgagcg ctcgcctcgg gcacgagctg
     2161 gttcgaggag ccctcgatcc gcggagcgtc gaagcccagc acggccaccg ccggcttcga
     2221 caacagccgg tcggcgatcg tccctgtgcc gagcagctgc acggagtcga gcacgcgcga
     2281 ctcctcccgg agttcatcct cgggcacctg catgcccgtc cacgtgaagg tgtgcagccc
     2341 ttcgatcgcc acgtcgccgt gctcgtcgtg cagcgtggcg atcacgcggc tcagcgcggt
     2401 gatcgcatcg gggatcggcc cgccgtaggc gccgctgtgc tgggcgatcg gcaggacgcg
     2461 cacggtgacg acgcagtcgg tgacgccccg cacgctcgtg ccgatcgtgg ggacaccggt
     2521 gcggtagttg ccgccgtcgg ccacgatcgc gacgtcggcg cgcagcagat cgccctggcc
     2581 gccgaccagt tcggggaggt gctcggtgga gcattcttct tcgccctcga cgaggatcgt
     2641 cacggtgacc gggagcgtgg cgccttcatg gactcccagg gcgcgcagcg ctgcggcgtg
     2701 gatcgcgatg ccggccttgt cgtcggcggc gccac
//
LOCUS       DATDBZ010000343         2199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1552698, whole genome shotgun sequence.
ACCESSION   DATDBZ010000343 DATDBZ010000000
VERSION     DATDBZ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552698"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VIX62_08285"
     CDS             complement(<1..324)
                     /locus_tag="VIX62_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004980205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5860267.1"
                     /translation="MYGALQDDLTSRLAEVREEGLYKNELVITTPQSAHVEVEGRGEM
                     LNLCANNYLGLASHPEIVAAAHEALDRWGFGMASVRFICGTQAVHRELEERLSAFLGT
                     EDTILY"
     gene            complement(335..1366)
                     /gene="tdh"
                     /locus_tag="VIX62_08290"
     CDS             complement(335..1366)
                     /gene="tdh"
                     /locus_tag="VIX62_08290"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY5860268.1"
                     /translation="MRALVKARAEPGLWLEEVPEPVIGINDVLIRVRRTGICGTDLHI
                     HRWDEWARQTIPVPMVVGHEFVGEIVAVGSNVSDFHAGDVVSGEGHVVCGRCRNCMAG
                     RRHLCAHTIGLGVNRPGAFAELVALPMTNIWHHWPGVDEEVAAIFDPFGNAVHTALAF
                     PVLGEDVLVTGAGPIGCMATAVVRHAGARHVVVTDLNPYRLELASRMGATLVVDARSR
                     DLHDVQRELGMTEGFDVALEMSGSEAALRSILDNMAHGGRIALLGIPAGEIGIDFNRI
                     VFDMLTIKGIYGREMYETWYQMSVMLQSGLDITPVITHRFAYDEFEEAFRIAGSGESG
                     KVILDWTDA"
     gene            complement(1428..2096)
                     /locus_tag="VIX62_08295"
     CDS             complement(1428..2096)
                     /locus_tag="VIX62_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860269.1"
                     /translation="MRALAVVVAVPLALVGWQWWSDRSAERMLTPIASQVAGREVVVD
                     CQSLWANLLDALPRHGEVRFDANGVPEPRILLTHQTCDRLQAFADSARHGELDCLRSL
                     DWSQPIALHPRSECYARSSRTVYALMTLAHEAYHTAGEMNEAVTNCYAIQAMAFVSVR
                     LGAERDEAELVARAMAVLAPLQSGGYGTDRCRAGTELDLHPETRAFPTEDPVVAPKGD
                     RGRS"
BASE COUNT          340 a          802 c          708 g          349 t
ORIGIN      
        1 gtagaggatc gtgtcctcgg tgccgaggaa ggccgacagc cgctcctcga gctcccggtg
       61 gacggcctgc gtgccgcaga tgaacctgac cgaggccatg ccgaagcccc aacggtcgag
      121 cgcctcgtgt gcggcggcga cgatctccgg gtggctggcc aggccgaggt agttgttcgc
      181 acagaggttg agcatctcgc cgcgtccctc cacctcgacg tgcgccgact gcggcgtcgt
      241 gatcaccagc tcgttcttgt acaacccttc ctcgcggacc tcggccagcc gcgacgtcag
      301 gtcgtcctgc agcgctccgt acatgcgtgt cctctcaggc gtcggtccag tcgaggatca
      361 cctttccgga ctcgccggag ccggcgatgc gaaaggcttc ctcgaactcg tcgtaggcga
      421 agcggtgcgt gatgacgggg gtgatgtcga ggccggactg cagcatgacg gacatctggt
      481 accaggtctc gtacatctca cgcccgtaga tccccttgat cgtgagcatg tcgaacacga
      541 tccggttgaa gtcgatccct atctcgcccg ccgggatccc gagcagtgcg atgcgaccac
      601 cgtgcgccat gttgtccagg atcgaccgca gcgccgcctc gctgccggac atctcgagcg
      661 ccacgtcgaa gccctcggtc atgcccagct cgcgctgcac gtcgtgcagg tcgcggctgc
      721 gcgcatcgac gaccaatgtc gcgcccatgc ggctcgcgag ctcgagccgg tacgggttca
      781 gatcggtcac cactacatgg cgggcgcccg cgtgacgcac gaccgccgtc gccatgcagc
      841 cgatcggccc cgccccggtg acgagcacat cctcccccag caccgggaag gcgagcgccg
      901 tgtgcactgc gttcccaaag gggtcgaaga tcgccgccac ctcctcgtcc acacccggcc
      961 agtggtgcca gatgttcgtc atcggcagcg ccaccagctc ggcgaacgcg cctgggcggt
     1021 tgacccccag cccgatcgtg tgcgcacaca gatgacgacg gcctgccatg cagttgcgac
     1081 agcgcccaca cacgacgtga ccttcgcccg acacgacatc ccccgcgtgg aagtcgctga
     1141 cgttcgaccc caccgccacg atctccccca cgaactcgtg ccccaccacc atcggcaccg
     1201 gaatcgtctg ccgcgcccac tcgtcccagc ggtgaatgtg cagatcggtg ccgcagatcc
     1261 ccgtcctccg caccctgatc agcacgtcgt tgatcccgat caccggctcc ggcacctcct
     1321 cgagccacag acccggttcc gcgcgtgcct tgacgagcgc cctcaccgcg cggtgattct
     1381 acggccgagc ctggggttgc acctcttgta ccgccgctga tcggccccta cgaccgcccg
     1441 cggtcgccct tcggcgcgac gaccggatcc tcggtcggga aggcgcgtgt ctccggatgc
     1501 aggtcgagct cggtgccggc ccggcagcgg tcggtgccgt agccgccgct ctgcagcggc
     1561 gcaagcaccg ccatcgcgcg ggcgacgagc tccgcttcgt cgcgctccgc gccgagccgc
     1621 accgacacga acgccatcgc ctggatggcg tagcagttcg tcaccgcttc gttcatctcg
     1681 ccggccgtgt ggtacgcctc gtgcgccagg gtcatcagcg cgtagaccgt ccgcgaggat
     1741 cgggcgtagc actccgagcg cggatggagc gcgatcggtt gggaccagtc gagcgagcgc
     1801 aggcaatcga gctcaccgtg tcgcgcgctg tcggcgaagg cctgcaaccg gtcgcaggtc
     1861 tggtgggtga gaagaatgcg aggctccggc acgccgttgg cgtcgaagcg cacctcgccg
     1921 tgtcgaggga gcgcgtcgag gaggttggcc cacaggctct ggcagtcgac caccacctcg
     1981 cggcccgcga cctgcgaggc gatgggcgtc agcatccgct ccgcagagcg gtcgctccac
     2041 cactgccagc ccacgagcgc gagtggcacg gcgacgacga cggcgagcgc cctcatctcc
     2101 gggatatcgg cagggttgcg ccgccactgc aggggactgc cccggatggg gacagtcccc
     2161 tcgggtcgct tacgccgccg ggatgatgtt ctggttcac
//
LOCUS       DATDBZ010000344         3014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_96359,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000344 DATDBZ010000000
VERSION     DATDBZ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96359"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VIX62_08300"
     CDS             <1..967
                     /locus_tag="VIX62_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY5860270.1"
                     /translation="STEDVLPRMSKVLQGATDSERADVWLIVGNSLRVRGSWPHEAPV
                     PPAVTAVGETLPELPGSFAAEVRHQGELLGALTVSMPVNDPLDPARERLVRDLAAQAG
                     PVLANVRLIEELRASRQRMVAAQDEGRRRLERNIHDGAQQQLVALSVKIRLADSMIDR
                     DAAKAHEMLSQIQADANDALETLRDLARGIYPPLLADKGLSAALAAQGRKASLPVTLD
                     ADGIGRYPAETEATVYFCVLEAMQNVAKYAQASGIELRLRTSNGALNFKVQDDGVGFD
                     PHRHTPGTGIQGMQDRLDAVGGSLQVSSAAGEGTIVIGSVPLSEA"
     gene            complement(997..1371)
                     /locus_tag="VIX62_08305"
     CDS             complement(997..1371)
                     /locus_tag="VIX62_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860271.1"
                     /translation="MSVRVLIVDDQEPFRMAARMVVDATDGFDVVGEAETGEDSVTLA
                     TELQPDLVLMDVNLPGINGLDATRQILSGSDTVVVLLLSTYEEDEYAPKAAECGAASY
                     IPKAVFGPDRLEDAWSAANASK"
     gene            complement(1485..>3014)
                     /locus_tag="VIX62_08310"
     CDS             complement(1485..>3014)
                     /locus_tag="VIX62_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5860272.1"
                     /translation="WLGCGALLGLLAWLVAWSIRSLTGGEGAVTWWLALGAIVLVPAV
                     ASLVSPRVGRLADPALVAVLAAYGVAAIVGVALLVMILIAGRIPDADAIRPFVVPTVM
                     AIVLAVVLALIFEPRLTRAAQHAVYGRTAGSADLARTFGSRMTRAIPLDELALQGAEA
                     LREALALRRVELWIPAGEELRPWIGDPPVERTAVPMGGLEPTAVVQAGLIGHGWMKVW
                     LPGLLTGREDAFVRAAPMAHAGELLGLLLVERPVGAEPFTPEDERTVVELARQLGVAV
                     YNARLDSALQASLDEVRRQADELQRSRGRIVAAGDQARRTIERNLHDGAQQHLVALAV
                     QLKLARSLMDRDPEQAKTSIDGLAEAVQDTIQELRDLAHGIYPPLLADKGLPDALVSA
                     GRRSVLPVTVETPDLGRYPAEAEATVYFCVLEALQNAGKYAGERAEVAVRVTEDAHTL
                     TFTVSDDGPGFDPRAGHMGAGFTNMLDRLGALGGSLRVESAEGKGTRVVGLVPAQRLD
                     A"
BASE COUNT          509 a         1041 c         1028 g          436 t
ORIGIN      
        1 ctccaccgag gacgtccttc cgcgcatgtc gaaggtcctc caaggagcga ccgactcgga
       61 gcgggcggat gtgtggctca tcgtcggaaa ctcactgcgc gtgcgggggt catggcccca
      121 cgaagcgccc gtgccaccag cggtgaccgc cgttggcgag acgttgcccg agctccctgg
      181 gtcattcgcc gccgaggtgc gacatcaagg cgagttgctg ggcgcgctca ccgtctcgat
      241 gccggtgaac gatccactcg acccggcgag ggaacgcctg gtacgcgatc tcgcggcgca
      301 ggctggaccc gtgctcgcca atgtgcggct catcgaagag ttgcgcgctt cgcgtcagcg
      361 catggtcgcg gcccaggacg agggcagacg acgactggag cggaacatcc acgacggcgc
      421 gcaacagcag ttggttgcgc tgtcggtgaa gatccgactc gcggactcga tgattgaccg
      481 cgacgccgcc aaggcgcacg agatgctgag ccagatccag gccgatgcga acgatgcgct
      541 cgagaccctg cgagacctcg cgcggggaat ctatccaccg ctgctggcgg acaaaggact
      601 gtcggcggcg ctcgcggcac aggggcgcaa agcatcgctc cccgtaacgc tggacgccga
      661 cggcatcgga cgctatcccg cggagaccga ggccaccgtg tacttctgcg tcctggaggc
      721 catgcagaac gtcgcgaagt acgcgcaggc gtcggggatc gagctccggc tgcggacgtc
      781 caacggcgct ctgaatttca aggtgcagga cgacggcgtg ggcttcgacc cgcacaggca
      841 cacgccgggc acggggatcc aggggatgca ggaccggctg gatgccgtcg gaggttcact
      901 gcaggtgagc tcggctgcgg gcgagggcac gatcgtcatc ggttcagtgc cgctctccga
      961 ggcgtgacgc gggcgccggc ggtagtctga gctggactac ttcgaggcgt tggcggccga
     1021 ccaggcgtct tcgagacggt cggggccgaa caccgctttc gggatgtagg acgctgcacc
     1081 acactcggcg gccttaggcg cgtattcatc ctcttcgtac gtggagagca ggagcaccac
     1141 cacggtgtcg gaaccactca ggatctgccg ggtggcatcg aggccgttga tccccggaag
     1201 gttcacgtcc atcagcacga ggtcgggctg cagctcggtg gccaaggtga ccgagtcctc
     1261 gccggtttcg gcttcaccga cgacgtcgaa gccgtcggtg gcatcgacga ccatgcgcgc
     1321 cgccatgcgg aacggctcct ggtcatcaac gatgaggacg cggacggaca taactgatgc
     1381 tcctgtggcg tgggcgatac aaccagcgtc ccaccgtagg tgccctccgc cagtagtgcc
     1441 agcaccactg ttgatgtagt cctggtcgct gcgagaacca ccgttcaggc gtcgaggcgc
     1501 tgggccggaa cgaggcccac gaccctggtg cctttgccct cggccgactc cacccgcagg
     1561 ctcccgccca acgctcccaa gcggtcgagc atattggtga acccggcgcc catgtgcccc
     1621 gcccgcggat cgaaccccgg tccgtcgtcg gagacggtga atgtgagggt atgcgcgtcc
     1681 tcagtgactc gcacggcaac ctccgcacgc tcccccgcgt acttgccggc gttctgcaac
     1741 gcctccagca cgcagaaata gaccgtggcc tccgcttcgg ctggataccg tccgaggtcg
     1801 ggcgtctcga ccgtgaccgg gagcaccgag cggcggcccg ccgacaccag ggcgtcgggc
     1861 aggcccttgt ctgccaggag cggcggatag atgccgtgcg ccaggtcgcg gagttcctgg
     1921 atcgtgtcct gcactgcttc ggccaacccg tcgatcgacg tcttggcttg ctcggggtca
     1981 cggtccatca acgaacgagc gagcttgagc tgcacggcga gcgccaccag atgctgctgc
     2041 gccccgtcgt gcaggttgcg ttcgatcgtt cggcgggcct ggtcgccggc agccacgatg
     2101 cggcctcgcg agcgctgcag ctcgtcggcc tggcgccgca cctcgtcgag cgatgcctgg
     2161 agcgcggagt ccaacctggc gttgtacacc gcgacaccga gctgccgcgc aagctcgacc
     2221 accgtgcgct catcctccgg cgtgaacggt tccgcaccca cggggcgttc caccaacagg
     2281 agtccgagca gctcaccggc atgcgccatc ggggcggcgc gcacgaaggc gtcctcgcga
     2341 ccggtgagca ggcccggcag ccacaccttc atccagccgt ggccgatcag tcccgcttgc
     2401 accaccgcgg tgggctcgag gccgcccatg gggacggccg tgcgttccac cggcggatcg
     2461 ccgatccacg ggcgaagctc ctcgccggcg gggatccaga gctccacccg gcgcagcgcc
     2521 agcgcttccc gcagcgcttc ggcgccctgc aaggccagtt cgtccaacgg gatcgcgcgg
     2581 gtcatgcgcg aaccgaacgt cctggcgaga tcggccgaac cggccgtccg gccgtacacc
     2641 gcatgctgcg cggcgcgggt gaggcgaggt tcgaatatca gcgccaggac cacggcgagc
     2701 acgatcgcca tcaccgtggg taccacgaac ggtcggatgg catcggcatc ggggatccgc
     2761 cccgcgatca ggatcatcac gagcagggcg acacccacga tggcggcgac gccgtaggcg
     2821 gcgagcaccg caacgagcgc cggatcggcg agccgtccga cgcgcgggga caccaacgag
     2881 gccaccgccg gcaccaggac gatcgctccc agcgcgagcc accaggtgac ggcgccctct
     2941 ccaccggtca gcgagcggat ggaccatgcg accagccacg cgagcagtcc gaggagcgcg
     3001 ccacacccga gcca
//
LOCUS       DATDBZ010000345         4581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1959502, whole genome shotgun sequence.
ACCESSION   DATDBZ010000345 DATDBZ010000000
VERSION     DATDBZ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959502"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VIX62_08315"
     CDS             complement(<1..305)
                     /locus_tag="VIX62_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5860273.1"
                     /translation="MRKHRMFVLGAGLLALSLFAAACNDSGGDDDTGGSASASESSAP
                     KPELTVGVSGPFAENQLVAEMYAQVLENAGYTVSRELDIADRAVGNKALEDGEIDLKP
                     "
     gene            complement(459..1193)
                     /locus_tag="VIX62_08320"
     CDS             complement(459..1193)
                     /locus_tag="VIX62_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860274.1"
                     /translation="MNGFLDGIQWIVDPAHWSGVDGVPSRTWEHLQLCLLGLLLASLI
                     AIPLGLLVGHTRRGTFLTAQLANLGRAIPSFAVLSITYLIVIKISPKLAFGSVPTIAA
                     LVLLSFPVILINTMVGIQQVDGDAIEAARGMGMNGRQVLLHLELPLAMPLIMTGLRLA
                     AVLIVATAGLAALVAGGGLGRYVVDGFALQETDRVVAGAILIAILAIGTDVLFSFLAK
                     LLSPRLHSSDRGPAPSRAPSVTARPV"
     gene            complement(1201..1914)
                     /locus_tag="VIX62_08325"
     CDS             complement(1201..1914)
                     /locus_tag="VIX62_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860275.1"
                     /translation="MIAAGIALAGVVAQEDTEPLIRWIWITDHLDEIQRQALQHLYLT
                     VASVAIGFAIAFPLAVLASRRRRVAAPITWVLGVLYTIPAIAFIFLLLPITGFSLTTV
                     VIPLIAYSLLILFRNTLAGLDGVDADVKEAAVGMGLSRRQVLWKVEIPLALPVIIAGL
                     RIATVSAIGLVTIAALIGRGGFGQFIQDGLQTFFWTPLLVGVVLSVLLAFLADVLLLL
                     VQRLTTPWTRGSGTRVLTT"
     gene            complement(1911..3014)
                     /locus_tag="VIX62_08330"
     CDS             complement(1911..3014)
                     /locus_tag="VIX62_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5860276.1"
                     /translation="MTAMISLQQIEKRYPGTTAPAVDGLSLDIAEGETVALVGPSGCG
                     KTTTLKMINRLIEPTGGRLLLDGTDVMQQDPVVLRRGIGYVIQHTGLMPHRTIHQNMA
                     TVPKLLGWDDERTAPRIEELTEIFDLDPELLDRYPAELSGGQRQRVGVARALAADPPV
                     MLMDEPFAAVDPIVRARLQDQFLDIQERLNKTIVFVTHDIDEAIKLSNRIAILNVGGK
                     LEQYAPAEEILREPANDFVREFVGAERGLKRLALIKVGDIPLEDGPVVAPTASADEAR
                     AAIEAGGFDFVSVIADGELLGWVDRDMLAGRASVADSTPRPFTAYVTARDSLRQALDS
                     IVTSRTGVAVVATEGQRYKGILTLERISQEIIS"
     gene            complement(3042..3659)
                     /locus_tag="VIX62_08335"
     CDS             complement(3042..3659)
                     /locus_tag="VIX62_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018485431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEY5860277.1"
                     /translation="MDASVPGILTLAEWRRRIAELYASVRADPDPEHAWRHWRDERRD
                     LFTTHPQSPIPEAERAGYGGPHVFDYDPTARVTAELRPLEAQPIEVVSSDGRTERFLR
                     FAEAAFTLYGQDATLEVFWLDAYGGGIYLSFRDATSGGSTYGGGRYLLDTVKGADLGA
                     VGYRPILDFNFAYQPSCSYDPQWSCPLPPPANRMALEVPAGERLG"
     gene            3786..3962
                     /locus_tag="VIX62_08340"
     CDS             3786..3962
                     /locus_tag="VIX62_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860278.1"
                     /translation="MDRNTIVAQMRAASSPNDICNAINAARAWLADHPEDDELRTSLQ
                     HLMRAEREHFAVRR"
     gene            complement(3988..>4581)
                     /locus_tag="VIX62_08345"
     CDS             complement(3988..>4581)
                     /locus_tag="VIX62_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amine oxidase"
                     /protein_id="HEY5860279.1"
                     /translation="TTAKVNLALSGLPAFNGTDDAERLKGRITVGLSIDHVERAMDAN
                     KYGHVAEDPMLEATIPTLVDPSLAPEGKHVMSILFQAAPRTLRDGDWAGERERVADIA
                     VKSMERFAPGLGELVEARQVITPEDMENDYGLTGGHVYHAEPGLDQFFAWRPLNGEAR
                     YRLQLEGLYLAGSGAHPGGGVTGGPGANAARQILADN"
BASE COUNT          780 a         1567 c         1518 g          716 t
ORIGIN      
        1 ggcttgagat cgatctcgcc gtcctcgagt gccttgttgc ccaccgcacg gtcggcgatg
       61 tcgagctcac ggctcacggt gtatccggcg ttctcgagga cctgcgcgta catctctgcc
      121 acgagctggt tctcggcgaa cgggccggac acgccgacgg tcagttcggg cttgggagcc
      181 gacgactcgc tggcgctggc cgagccgccg gtgtcgtcgt ccccacccga gtcgttgcac
      241 gcggccgcga agagcgacaa cgccaacaga cccgccccga ggacgaacat gcggtgtttc
      301 cgcatgggtc tatccctccg atcgaacatc gtgggggtgt ccacctcccc cggttggacc
      361 cgcgcaccct accaagggcg cgctcgtggt tgctacccag tgcaaaccag gttgaaccgg
      421 tgatgttccc gtggtcagga tgttccgcgg caaggaacct agaccggccg cgcggtcacc
      481 gatggcgctc gcgacggtgc gggaccacga tccgatgagt ggaggcgggg ggacaacagc
      541 ttggccagga acgagaacag cacgtccgtg ccgatcgcca agatcgcgat caagatcgcg
      601 ccggcgacga ccctgtcggt ctcttggagg gcgaacccgt ccacgacgta gcgacccaga
      661 ccaccgccgg ccaccagcgc cgccaatccc gccgtggcca cgatgaggac cgcggccaga
      721 cgtaggccgg tcatgatcaa cggcatcgcc aaggggagct ccaagtgcag cagcacctgc
      781 cgtccgttca tgcccatccc acgagccgct tcgatcgcat cgccgtcgac ctgctggatc
      841 cccaccatcg tgttgatcag gatcacgggg aaggaaagca gcaccaaagc ggcgatcgtg
      901 ggcaccgatc cgaaagcgag cttcggcgag atcttgatca cgatcaggta ggtgatcgag
      961 agcaccgcga acgacgggat cgcgcgcccg aggttggcca attgggccgt gaggaaggtt
     1021 ccgcgccggg tgtgccccac gaggagtccg agcgggatcg cgatgaggct cgccagcagc
     1081 aaccccagca gacacaactg caggtgctcc caggttcgcg acggcactcc gtccacgccc
     1141 gaccaatggg ccggatccac gatccactgg atgccgtcga ggaatccgtt catggccgcc
     1201 tcatgtggtc agtacccgcg tgcccgagcc cctcgtccac ggcgtcgtca gccgttggac
     1261 gagcaggagg aggacgtcgg ccaggaacgc cagcaggacc gacagcacca cgccgaccaa
     1321 caatggtgtc cagaagaagg tctggagccc atcctggatg aactgcccga acccgccgcg
     1381 gccgatcagc gcggcgatcg tcacgaggcc gatcgcggac acggtggcga tccggagccc
     1441 ggcgatgatc accgggagcg ccagcgggat ctcgaccttc cacaggacct ggcgtcgaga
     1501 caggcccatc cccacggccg cttccttcac gtcggcatcg acaccgtcca gtcctgcgag
     1561 cgtgttccgg aacaggatca gcaggctgta ggcgatcaat gggatgacca cggtggtgag
     1621 cgagaacccg gtgatcggca gcaacaggaa gatgaacgcg atcgccggga tcgtgtacag
     1681 gacccccaag acccacgtga tcggcgccgc cacacgacga cgccgggaag ccagcaccgc
     1741 gagcgggaat gcgatcgcga acccgatggc caccgaggcc accgtgaggt acaggtgttg
     1801 cagggcctgg cgttggatct cgtcgagatg gtcggtgatc cagatccatc ggatcagcgg
     1861 ttcggtgtcc tcctgggcga cgacacccgc gagcgcgatc cccgctgcga tcacgagatg
     1921 atctcctgcg agatccgctc cagcgtcagg atccccttgt agcgctggcc ttcggtggcc
     1981 accaccgcca ccccggttcg cgacgtcacg atcgaatcca gcgcctgccg caacgaatcg
     2041 cgagccgtga cgtacgcggt gaacggcctc ggcgtggagt cggcgacgct ggccctgccc
     2101 gccagcatgt cccggtccac ccagcccagc agctcaccgt cggcgatcac gctgacgaaa
     2161 tcgaacccgc cggcctcgat cgcggcgcgc gcctcgtcgg cggaggccgt cggcgccacc
     2221 acggggccgt cctcgagcgg gatgtcgccc accttgatca gggccaacct cttcaagcca
     2281 cgttcggcac cgacgaactc gcgcacgaag tcgttggcgg gctcacgcag gatctcctcg
     2341 gccggggcat actgctcgag cttgccgccc acgttgagga tggcgatccg gttgctgagc
     2401 ttgatcgcct cgtcgatgtc gtgggtcacg aacacgatcg tcttgttcag tcgctcctgg
     2461 atgtccagga actgatcctg cagccgggct cgcacgatgg ggtcgaccgc ggcgaacggc
     2521 tcgtccatga gcatcaccgg agggtcggcg gcgagggcac gagccacgcc cacccgctgt
     2581 cgctggccgc ccgagagctc ggcggggtat cgatccagca actcggggtc gaggtcgaag
     2641 atctcggtga gctcctcgat ccgtggagcg gtccgttcgt cgtcccagcc cagcagcttg
     2701 ggcacggtgg ccatgttctg gtggatcgtt cggtggggca tcaagccggt gtgctggatc
     2761 acgtagccga tcccacggcg cagcaccacc ggatcctgct gcatcacgtc ggtgccgtcg
     2821 agcagcagcc ggcctcctgt gggttcgatc agccggttga tcatcttcag cgtcgtggtc
     2881 ttgccgcatc ccgagggacc caccaacgcc acggtctcgc cctcggcgat gtccaggctg
     2941 aggccgtcga cggcgggcgc ggtggtgccc gggtagcgtt tctcgatctg ctggagcgag
     3001 atcatcgcgg tcatgggcac ccccgggcgg aaggcggcat cctagcccag gcgttcgccc
     3061 gccggaacct ccaacgccat gcggttggcg ggagggggca gcgggcagga ccactgaggg
     3121 tcgtaggaac acgatggctg gtaggcgaag ttgaaatcca ggatgggccg gtagcccacg
     3181 gcgcccagat cggcgccctt cacggtgtcg agcaggtatc gcccgccgcc ataggtgctg
     3241 ccgccgctgg tggcgtcccg gaacgagagg tagatgccgc cgccgtaggc atccagccag
     3301 aagacctcga gggtggcgtc ctggccgtag agcgtgaagg cggcctcggc gaaccggagg
     3361 aaccgttccg tgcgtccgtc gctgctgacc acctcgatcg gctgggcctc gaggggacgc
     3421 agttccgccg tcacgcgcgc cgtcgggtcg taatcgaaca cgtgcggacc cccgtacccg
     3481 gcccgctcgg cctctgggat cggcgactgc gggtgcgtgg tgaacaggtc tcggcgctca
     3541 tcgcgccaat ggcgccaagc atgttcggga tcggggtcgg cgcggacgga ggcgtagagc
     3601 tctgcgattc gccggcgcca ctcggcgagc gtgaggatcc ccggtaccga cgcgtccatc
     3661 gcccgaccct agtcgcgccc cacgggaatc gtccacgagg cccgatccgg gtcatgaggt
     3721 gacgatgatg ttgcaacggg ccacaggtgc ccttgagatc gcgtgcctgc gggtacggtc
     3781 gttgcatgga ccgaaacacc atcgtggcgc agatgcgcgc cgcctcatcc ccgaacgaca
     3841 tctgcaacgc gatcaacgcc gctcgcgcct ggctcgccga ccatcccgag gacgacgagc
     3901 tccgcacctc gctgcagcac ctcatgcgtg ccgagcgcga gcacttcgcc gttcgccgct
     3961 gaacacccgc gctccgcgca gtgctcccta attgtcggcg aggatctgtc gtgccgcgtt
     4021 ggcgcccgga ccaccggtga cgcctccgcc cggatgcgcg cccgagccgg ccagatacag
     4081 cccttcgagc tgcagccggt atcgcgcctc accgttcaac ggacgccagg cgaagaactg
     4141 atcgaggccg ggttcggcgt gatagacgtg cccacccgtg aggccgtagt cgttctccat
     4201 gtcctcgggc gtgatgacct gacgggcttc caccagttcc ccgagccccg gagcgaaccg
     4261 ctccatcgac ttcacggcga tatcggcgac gcgctcccgt tctccggccc agtcaccgtc
     4321 gcgcaaggtc ctgggtgcgg cctggaacag gatcgacatc acgtgcttgc cctctggcgc
     4381 cagcgaaggg tcgaccaacg tggggatcgt cgcctccagc atcgggtcct cggcgacgtg
     4441 gccgtacttg ttggcgtcca tcgcgcgctc gacgtggtcg atcgacaacc ccaccgtgat
     4501 ccggcccttg agccgttcgg catcgtcggt cccgttgaag gccggcaggc ccgagagcgc
     4561 caggttcacc ttggcggtgg t
//
LOCUS       DATDBZ010000346         7916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2341862, whole genome shotgun sequence.
ACCESSION   DATDBZ010000346 DATDBZ010000000
VERSION     DATDBZ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341862"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VIX62_08350"
     CDS             <1..689
                     /locus_tag="VIX62_08350"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY5860280.1"
                     /translation="GPVQVEGSTVRVERFQQEGFAGTVPGDPSSAAFLVAAAALTGSE
                     LTIDGVGLNPSRIHYLEVMERFGIHTEVRVDHDEVGEPVGQLWVAPCDGIRPTRIEAW
                     ELPLVIDEVPVLALVAAHARGDSWFLGASELRVKESDRLAGVADGILALGGHAAAEGD
                     DLVVAGGGLEGGRGDGQGDHRMAMAFAVSALAARRPAEVDGMEAADVSFPGFVPTLIG
                     LGASIEVVSA"
     gene            686..1351
                     /gene="cmk"
                     /locus_tag="VIX62_08355"
     CDS             686..1351
                     /gene="cmk"
                     /locus_tag="VIX62_08355"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY5860281.1"
                     /translation="MNATRPRVVAIDGVAGSGKSTLARGLARGLGLPYVNTGLMYRAL
                     AASALDHEVATDDEAALVELTRRLTFTVVEGDPSQLEVEGFPANRLMTLEVEAVVSSV
                     ARHPGVRTLMRATQRAIGERDGAVMEGRDIGSVVFADAPVKLYLSADVDQRAVRRADQ
                     RRAGRAQVERSLTDRDALDARTTPHVPADDAVIIDTTDLTIDRTLLAALDVVARLAPE
                     LLS"
     gene            1348..2664
                     /gene="der"
                     /locus_tag="VIX62_08360"
     CDS             1348..2664
                     /gene="der"
                     /locus_tag="VIX62_08360"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY5860282.1"
                     /translation="MSARAPSPRIAVVGRQNVGKSTLANRLFGRREAIAHDMPGVTRD
                     RVELEAVWRGKRFGLVDTAGYVQDATGVEELARVQADTAMRDADLILLIVDAQAGITE
                     ADAALARRLRRASVPVLVVANKVDGAAEESDAAAFHALGLGEPVAVSGMHGRATGDLL
                     DRIVELLPDAPEVREDDDLEPRFALVGRPNVGKSSLFNRLLGRERSVVFEEAGTTRDS
                     VDDVIEWPTGSVRFVDTAGMRRQVKVKGVEYFSFVRATEAIDRANVVILVIDAVEGFT
                     VEDKKIANRVMDAGRAFMLVANKWDLVEEKDARYQELAETVRPFANATVMRASAKTGR
                     GVHRLPPLLLDLHERWTSRISTSKINEVIQQAQRERPTPRVAGTLHYATQVATGPPSF
                     VIFGGANEPDGTYQRYLENRLRRTFDLDGVPIRLKFRPRKRHSKQR"
     gene            2700..2774
                     /locus_tag="VIX62_08365"
     tRNA            2700..2774
                     /locus_tag="VIX62_08365"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2735..2737,aa:Pro,seq:ggg)
     gene            2778..3365
                     /locus_tag="VIX62_08370"
     CDS             2778..3365
                     /locus_tag="VIX62_08370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY5860283.1"
                     /translation="MKGTAEPTGGSSAVLTVPNLISAARIALIPVFVALIVDPDTTLA
                     GVLLFGLVVATDWVDGTIARRTGQVSELGKILDPVADRLAIAAGLIALMVRDVFPIWA
                     GLLILVRDAAVLVVGLVALRRFHTRLDVRFIGKVATFSLMVSIPAIAWGNLGTWPAAA
                     ALAVGWICYTAGIVEYYLAAGIYAADLRRAMRTHE"
     gene            3428..3814
                     /gene="gcvH"
                     /locus_tag="VIX62_08375"
     CDS             3428..3814
                     /gene="gcvH"
                     /locus_tag="VIX62_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY5860284.1"
                     /translation="MEFPEDLRYTKEHEWARADGTRVRIGITDFAQDALGDVVYVDLP
                     EVGAEVTASQAFGEVESTKSVSDVFSPLTGTIVERNPLLDDRPELVNEQPYGDGWLIV
                     IEVGDPAQVEQLLDAAAYQAFIQQGS"
     gene            3927..4463
                     /locus_tag="VIX62_08380"
     CDS             3927..4463
                     /locus_tag="VIX62_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5860285.1"
                     /translation="MSVDRPGCTLDPDARAQTDRDEDLTLFCTRCGHPNRDDARFCAE
                     CGAPLQGDPTLSLSAVEPEDEGHDEFPFPHDELGPGQGLLLVKRGPNAGSTFLLEDDV
                     TPVGRDPETAVFLDDITVSRKHAAFERREASEWFVRDVGSLNGTYVNGEQVDETKLAS
                     GDEVQVGKFKLTFFAAGE"
     gene            4463..5212
                     /locus_tag="VIX62_08385"
     CDS             4463..5212
                     /locus_tag="VIX62_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5860286.1"
                     /translation="MEQMAGTRNYQSIGEVLVSVKTEFPDITISKIRFLEAEGLIEPE
                     RTPSGYRKFYVDDVDRLKSILRMQRDEYLPLKVIKERLLTQDAADVAGEGLEGAELDA
                     DLDDEADAANATAEIADAPTGLQMSIEEMSAATGVERDRIKELESFGIVCSHGPDGAK
                     YYDGDDYIILSIVKDFFRFGIEPRHLTMYKHFADRETSFFEALVAPTLRQKNPDARKA
                     AAQTLTDLSVTSRKFKQALLRTNLRNDLQSA"
     gene            5209..6480
                     /locus_tag="VIX62_08390"
     CDS             5209..6480
                     /locus_tag="VIX62_08390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY5860287.1"
                     /translation="MRLARVRRGAVAVVAVIAVGMPFVTGLAVAGPAGPPPTPVPPNG
                     SPSPFPQSLQTPQDATARPDLDVPVAYLADLDDGQAMFAKAPMARRPIASLTKIMTAL
                     LTLERTSLDDVVTITPEAVFGRDDFGATSSLGLHAGEKLTVRDLLYALMLQSSNDAAV
                     ALAIEVAGSEAAFVRLMNRRAATLGMDGTVFFSPNGLDDRGRSSALDLTRLARVAYDT
                     PGFAPIVATKFRTIPAPPGGKPRKIQNRNALLWLYPGAVGVKTGSTAGAGYCVVAVAE
                     RGGRRLISVVLGSGNEPFSEAATLFDYGFEAFEERTFVTAGQDLGTVAIRGGVVPVEA
                     GAALEGLVPAEAGQAKRRIVVSPEAVFPPAADERIGTLKVTIPGVTVGAVPVVVSDVP
                     APPAAGDGPWWARASGAVVEGIGDVFRGIFG"
     gene            6507..7082
                     /gene="hpt"
                     /locus_tag="VIX62_08395"
     CDS             6507..7082
                     /gene="hpt"
                     /locus_tag="VIX62_08395"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019240087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY5860288.1"
                     /translation="MSEVASVLFGREDVRRRVRELGRAITGDYVGRAPVFITVLRGGA
                     MFLADLMREVDLPVEVHFMAISRYGDAAESLGRVQILLDVDADLADRDVILVEDIVDT
                     GLTLRFLLGSLRARGPASLEVCTLLDKAVRRIVPLTPRYAGFECPDRFVVGYGLDFAE
                     RYRNLPDILQVDDLEALKADPDVLAPLLATV"
     gene            7141..7614
                     /locus_tag="VIX62_08400"
     CDS             7141..7614
                     /locus_tag="VIX62_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEY5860289.1"
                     /translation="MIEMELTGVRVELPTNQPIVLLREREGERYLPIWIGAAEAAAIA
                     LSLQGVVTPRPMTHDLLKTILEDLTVQVRRIVVTELRESTFFATIELDRQGQELEVSS
                     RPSDAIALAVRMSVPIFASEDVLNEASILIPGDEEEEVEKFREFLDNVSPEDFES"
     gene            complement(7730..>7916)
                     /locus_tag="VIX62_08405"
     CDS             complement(7730..>7916)
                     /locus_tag="VIX62_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860290.1"
                     /translation="AHCYAWGENVGWTTGDVPDLEKAFMASAGHREHILDRTFRRAAV
                     GAVKIKGKLWVTVFFCT"
BASE COUNT         1228 a         2707 c         2731 g         1250 t
ORIGIN      
        1 gtggcccggt ccaggtcgag ggttcgaccg tgcgcgtgga gcgcttccag caggagggct
       61 tcgcggggac cgtgcctggc gatccctcat cggccgcctt cctggtggcc gccgccgcgc
      121 tcaccggcag cgaactcacg atcgacgggg tcggcctcaa cccgagccgc atccactacc
      181 tcgaggtgat ggagcggttc ggtatccaca cggaggtccg cgtcgaccac gacgaggtcg
      241 gcgaacccgt cggccagctg tgggtggccc cgtgcgatgg gatccgcccg acgcgcatcg
      301 aggcgtggga actcccgctg gtgatcgacg aagtgccggt cctcgccctc gtggccgcgc
      361 acgcgcgggg cgacagctgg ttcctgggcg cgagcgagct gcgggtgaag gagtccgatc
      421 gcctggcggg tgtcgccgac ggcatccttg ccctgggtgg acacgcggcg gccgagggtg
      481 acgacctcgt ggtggcgggc ggagggctcg agggcgggcg cggcgatggg cagggcgatc
      541 atcggatggc gatggccttc gcggtctcgg ccctggcggc ccggcgtccg gccgaggtcg
      601 acggcatgga agcggccgac gtgtcgttcc caggcttcgt gccgacgttg atcgggctcg
      661 gcgcatcgat cgaggtcgtg agcgcgtgaa cgcgacccgt ccgcgcgtcg tggcgatcga
      721 cggtgtcgcc ggcagtggca agagcacgct cgctcgtggc ctggctcgag gcctggggct
      781 gccatacgtc aacacgggat tgatgtaccg ggcgctcgcg gcctctgccc tcgaccacga
      841 ggtcgccacc gatgacgagg cagccctggt cgagctgacg cgccgcttga cgttcacggt
      901 cgtcgagggt gacccgtccc aactggaggt cgagggcttc cctgcgaacc gtctgatgac
      961 cctcgaggtc gaggccgtcg tctcgtccgt cgcgcggcat cccggggtgc ggaccctgat
     1021 gcgggccacc caacgcgcga tcggtgaacg cgacggtgcg gtgatggagg gtcgagacat
     1081 cggctcggtg gtgttcgccg acgcacccgt caagctgtac ctgtccgccg acgtcgatca
     1141 acgtgccgtg cgtcgcgccg accagcgccg ggcgggccgg gcccaggtcg aacgctcgct
     1201 cacggatcgc gatgccctgg atgcgcgcac cacgccgcac gtccccgccg atgacgccgt
     1261 catcatcgat accaccgacc tcacgatcga tcgaacgctc ctggccgccc tcgatgtcgt
     1321 cgcgcgcctg gcgccggagc tgctctcgtg agcgcgcggg ccccttcccc acgcatcgcg
     1381 gtggtcggcc gtcagaacgt ggggaagtcg acgctcgcca accgcctgtt cgggcggcga
     1441 gaggcgatcg cccacgacat gcccggcgtc acgcgcgacc gggtggaact cgaagcggtc
     1501 tggcgcggca aacggttcgg tctggtcgac accgccggat acgtgcagga cgcaaccggc
     1561 gtcgaggagc tcgcgcgggt ccaagccgac accgcgatgc gggacgccga cctcatcctg
     1621 ctcatcgtcg acgcgcaggc ggggatcacc gaagccgatg ccgcgctcgc ccgtcggctc
     1681 cgccgtgcga gcgttcccgt gctggtcgtc gcgaacaagg tcgacggtgc cgcggaggaa
     1741 tccgacgccg cggcgttcca tgccctgggg ctgggggaac cggtcgcggt gtccggcatg
     1801 cacggacgcg ccaccggcga tctgctcgat cgcatcgtcg aactgctccc ggacgccccg
     1861 gaggtacgcg aggacgacga cttggagccg aggttcgcct tggtcggccg accgaacgtc
     1921 ggcaagtcga gcttgttcaa ccggctcctc ggccgggagc gctccgtcgt gttcgaagag
     1981 gccggcacga cgcgcgactc ggtcgacgac gtgatcgagt ggccgaccgg gagcgtgcgg
     2041 ttcgtggaca ccgcggggat gcggcgtcag gtcaaggtca agggtgtcga gtacttcagc
     2101 ttcgtgcgcg cgaccgaggc gatcgatcgc gcgaacgtcg tcatcctggt gatcgatgcc
     2161 gtcgaaggct tcacggtcga agacaagaag atcgcgaacc gggtgatgga cgcgggtcgg
     2221 gcgttcatgc tcgtcgccaa caagtgggac ctcgtcgaag agaaggacgc tcgctatcag
     2281 gagctcgccg agacggtgag accgttcgcg aacgcgacgg tgatgcgagc atcagccaag
     2341 accggacgtg gtgtgcaccg tctgccaccg ctcctgctcg acctccacga gcggtggacg
     2401 tcgaggatct cgacctcgaa gatcaacgaa gtgatccagc aggcgcaacg cgagcgaccc
     2461 accccgcgcg tcgcgggcac ccttcactac gcgacgcaag tcgccaccgg accgccgtcg
     2521 ttcgtgatct tcggtggcgc caacgaaccg gacggcacct atcagcgcta cctcgagaat
     2581 cgcctccgcc gaaccttcga tctcgatggt gttccgatcc gcctgaagtt ccggccacgc
     2641 aagcgacaca gcaagcagcg ctgaggcggc cgtccgtgcg accggtacaa tcacctcttc
     2701 gggccgtggc gcagcttggt tagcgcgtct gtctggggga cagaaggtcg ccggttcaaa
     2761 tccggccggc ccgacgcatg aaagggacgg cggagcccac cggtggatcc agcgcggtcc
     2821 tcaccgttcc gaacctgatc tccgcggcac ggatcgccct catccccgtg ttcgtcgccc
     2881 tgatcgtcga ccccgacacc acgctcgccg gcgtcctgct gttcgggctg gtggtggcca
     2941 ccgactgggt cgacggcacg atcgcccgca ggaccggaca ggtgtcggag ctggggaaga
     3001 tcctcgaccc ggtggccgac cgactcgcga tcgccgccgg cttgatcgcg ctcatggtcc
     3061 gcgacgtgtt cccgatctgg gcgggcctgc tgatcctcgt ccgcgacgcc gcggtcctgg
     3121 tggtcgggct cgtcgcgctc cggcggttcc atacgcgtct ggacgttcgc ttcatcggca
     3181 aggtcgccac cttctccttg atggtgtcga tccccgcgat cgcgtggggg aacctgggca
     3241 cctggccggc cgccgccgcg ctcgccgtcg gatggatctg ctacacggcg ggcatcgtcg
     3301 agtactacct ggcagcaggc atctacgctg ccgacctgcg acgggcgatg cgaacgcacg
     3361 agtgaaaggc agtatcgttc cgccgacggc gccgcaccgg gaccgtcggc atgaaaggga
     3421 ggatgacgtg gagttccccg aggaccttcg gtacacgaag gagcacgagt gggcgcgcgc
     3481 cgatggcacg agggtccgca tcggcatcac cgacttcgcc caggatgccc tcggggacgt
     3541 ggtctacgtc gacctgccgg aggtgggcgc cgaggtgacg gcgagccaag ccttcggtga
     3601 ggtggaatcc accaagtcgg tgtccgatgt cttcagcccg ctcacgggca cgatcgtcga
     3661 gcgcaacccg ctgctggacg atcgtccgga gttggtgaac gagcagccgt acggcgacgg
     3721 ctggctgatc gtgatcgagg tcggcgaccc ggcgcaggtc gagcagctcc tggatgccgc
     3781 cgcctaccag gcgttcatcc agcagggctc gtagcgctcc atcgagactt caccccgtcc
     3841 agcgagggtc gagcctgacc ctggtgcccg gtcgagggtt gagcccccgt acggttgaag
     3901 gtcgacccca cctgcgataa cgtccagtgt ccgtcgaccg tccaggttgt accctcgacc
     3961 ccgacgctcg tgcacagacc gaccgtgacg aggacctgac gttgttctgt acccgctgtg
     4021 gacatcccaa ccgagacgac gcgcgcttct gtgccgagtg tggtgctcca ctgcaagggg
     4081 accccacgct ctcgctgagc gccgtcgagc ccgaggatga ggggcacgat gagttcccct
     4141 tccctcacga tgaactgggg cccggacagg gtctgcttct ggtcaagcgt ggccccaacg
     4201 cggggtcgac gttcctcctc gaggacgacg tgaccccggt gggtcgcgat cccgagaccg
     4261 cggtcttcct cgacgacatc acggtctcgc gcaagcacgc ggcgttcgaa cggcgagaag
     4321 catcggagtg gttcgtccgc gacgtcggca gcctcaatgg cacgtacgtg aacggcgagc
     4381 aggtcgacga gacgaagctc gcgtccggtg acgaggtcca ggtcggcaaa ttcaagttga
     4441 cgttcttcgc ggcaggggag tgatggagca gatggcgggc acccgcaact accagtcgat
     4501 cggcgaggtc ttggtctcgg tcaagaccga gttccccgac atcacgatct ccaagatacg
     4561 gttcctcgaa gccgaagggt tgatcgaacc cgagcgcacg ccgtcgggct accgcaagtt
     4621 ctacgtcgat gatgtcgacc ggttgaagtc gatccttcgg atgcagcgcg acgagtacct
     4681 ccccctgaag gtgatcaagg aacgtttgct cacgcaggac gccgccgacg tagcggggga
     4741 gggcctcgag ggcgcggagc tcgacgccga cctcgacgat gaagccgacg ccgccaacgc
     4801 caccgccgag atcgccgatg cccccaccgg cctgcagatg tcgatcgagg agatgtcggc
     4861 ggccaccggg gtcgagcgcg atcgcatcaa ggagctcgag tccttcggca tcgtgtgctc
     4921 gcacggtccc gacggcgcga agtactacga cggggacgac tacatcatcc tgtcgatcgt
     4981 gaaggacttc ttccggttcg gcatcgaacc gagacacctc acgatgtaca agcacttcgc
     5041 cgatcgcgag acgtcgttct tcgaagcgct cgtggcgccc accctgcgtc agaagaaccc
     5101 cgacgcgcgg aaggccgccg cccagaccct caccgacctg tcggtgacct cgcgcaagtt
     5161 caagcaggcg ctgctccgca cgaacctgcg caacgacctg cagagcgcgt gagactcgcg
     5221 cgcgtccgac ggggcgcggt ggccgtggtg gccgtgatcg cggtcgggat gccgttcgtc
     5281 accggactgg cggtcgccgg cccagccggc cccccaccca cgccggtgcc tccgaatggg
     5341 tccccctccc cgttcccgca atcgctccag acgccgcagg acgcgacggc tcggcccgat
     5401 ctcgacgtgc ccgtggccta cctcgccgac ctcgacgacg gtcaggcgat gttcgccaag
     5461 gcgccgatgg cccgccggcc catcgccagc ctcacgaaga tcatgacggc gttgctgacg
     5521 cttgagcgca cctcgctgga cgacgtcgtc accatcaccc ctgaggcggt cttcggacga
     5581 gacgatttcg gggcgacctc gagcctcgga ctgcacgcag gggagaagct caccgttcgc
     5641 gacctgctgt acgcgctgat gctgcagtcc tccaacgacg ccgccgtggc gctggccatc
     5701 gaggtcgcag gctccgaggc ggcgttcgtc cgcctgatga accggcgtgc ggccaccctc
     5761 gggatggatg gcaccgtgtt cttctcaccc aacggcctcg acgaccgagg tcgctcgagc
     5821 gccctcgacc tcacccgctt ggcgcgggtc gcctacgaca caccggggtt cgctccgatc
     5881 gtcgccacga agttccgcac gatcccggct ccgcccggtg gcaagccgcg gaagatccag
     5941 aaccgcaacg ccctgctctg gctgtacccg ggcgcggtcg gcgtgaagac gggctcgacg
     6001 gccggcgcgg gctactgcgt ggtcgccgtc gccgagcgcg gcgggcggcg gctgatctcg
     6061 gtcgtcctgg gctcgggcaa cgaacccttc tccgaagccg ccaccctctt cgactacgga
     6121 ttcgaggcct tcgaggagcg gaccttcgtg acggccggcc aggacctcgg cacggtggcg
     6181 atccgcggcg gcgtcgtccc ggtggaggcg ggggctgcgc tggagggctt ggtccccgcc
     6241 gaggccgggc aggcgaaacg tcgcatcgtg gtctcgcccg aggccgtgtt cccacctgcg
     6301 gccgatgagc ggatcggcac gttgaaggtg acgatccccg gggtcaccgt cggtgccgtg
     6361 cccgtggtgg tgagcgacgt ccccgcgccg ccggccgctg gagacgggcc gtggtgggcg
     6421 agagccagcg gcgccgtcgt cgaagggatc ggcgacgtgt tccgcggcat cttcggctga
     6481 gggaggagcc tgctaggctc cgcccgatgt cggaggtcgc gagcgtcctg ttcggtcgcg
     6541 aggacgtccg tcgccgggtg agggaactcg gccgcgcgat caccggggac tacgtgggcc
     6601 gggcacccgt gttcatcacg gtcctccggg gtggcgcgat gttcctcgcg gacctgatgc
     6661 gcgaggtcga cctgcccgtc gaggtccact tcatggcgat cagccggtac ggcgacgccg
     6721 ccgagtcgct cgggcgcgtg cagatcctgc tcgacgtgga cgccgacctc gccgatcgcg
     6781 acgtgatctt ggtggaagac atcgtcgaca ccgggctcac gcttcggttc ctgctgggtt
     6841 cgctgcgggc tcgtggaccg gcgtccctcg aggtgtgcac cctgctcgac aaggcggttc
     6901 gacggatcgt ccccctgacg ccccggtacg cgggcttcga gtgcccggac cgattcgtcg
     6961 tggggtacgg gttggacttc gccgagcgct accggaacct gcccgacatc ctgcaggtcg
     7021 acgacctcga ggcgctcaag gccgatcccg acgtgctcgc gcccctgctg gcgacggtgt
     7081 gaggcggggc gcagggctcc ggttgcccca cctgcacccc ccggttacgc tcgacccacg
     7141 atgatcgaga tggagctcac cggcgttcgc gtcgaactac cgaccaatca gccgatcgtg
     7201 ctcctgcgcg agcgagaagg ggagcggtac ctgcccatct ggatcggtgc cgccgaggca
     7261 gccgcgatcg cgttgtcgct gcagggggtg gtcacccccc ggcccatgac ccacgacctg
     7321 ctcaagacga tcctcgaaga tctcacggtg caggtgcgcc gcatcgtggt gaccgagctc
     7381 cgcgagtcca cgttcttcgc gacgatcgaa ctcgaccgtc agggtcagga gctcgaggtc
     7441 tcgtcccgcc cgtcggatgc gatcgcgctc gcggtgcgta tgtcggtccc catcttcgcc
     7501 agcgaagacg tcctcaacga agcctcgatc ctgatccccg gcgacgagga agaagaggtc
     7561 gagaagttcc gggagttcct ggacaacgtc agcccggagg acttcgagag ctagccggct
     7621 cccagggccg gatccgggcc cgaacacgcg agcggcgggc gtccgagcga ccccctcgac
     7681 cgctcgttcg cccgcccggc tcgcagtgaa cctgccgcgg gacgacaggc tacgtgcaga
     7741 agaacacggt gacccagagc ttgcccttga tcttcacggc gcccaccgcg gcgcggcgga
     7801 acgtgcgatc caggatgtgc tcacggtggc ccgcgctggc catgaaggcc ttctccagat
     7861 ccgggacgtc cccggtggtc caacccacgt tctcgcccca tgcgtaacag tgcgcg
//
LOCUS       DATDBZ010000347         2553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1959584, whole genome shotgun sequence.
ACCESSION   DATDBZ010000347 DATDBZ010000000
VERSION     DATDBZ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959584"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VIX62_08410"
     CDS             <1..1032
                     /locus_tag="VIX62_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5860291.1"
                     /translation="PSTMVFDDHDMIDDWNISEAWVQDIRREPWWPEHIIGGLMSYWI
                     YQHLGNLSPDEIDAEGLLARVQQADDARDVLRAWAFASDECTPLPGGYRFSCYRELGA
                     VRLVVIEARNARTLDRGKRAMVGDDEWAWVAELCRRPVGHLVIATSLPVFVPGGLHGL
                     QQWNEAVCDGAWGRPFAWLGERLRRALDLEDWPAFDRSFRAFVALLAEVVAPGPGGSP
                     STITVVSGDIHFSYVAGIQLAGSVEGSTRITQVVSSPIRNALTGRDRRVLQFGVSRFG
                     RWIGGWLQRRVGRGPSAATWELDDGPFFDNSMGELVFEGDAAKLLIERSRPDDDGEPI
                     LEIMVDHRL"
     gene            complement(1035..1334)
                     /locus_tag="VIX62_08415"
     CDS             complement(1035..1334)
                     /locus_tag="VIX62_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5860292.1"
                     /translation="MVGSTSLAAAPLPVPFAVLDIGTVTEFRAWAEAVAPREPATMLL
                     DLSGVDLVTAAGVSALLAFEHELPSGSQLRIVNELPIVRRVLAICELDERWLASS"
     gene            1421..2083
                     /locus_tag="VIX62_08420"
     CDS             1421..2083
                     /locus_tag="VIX62_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860293.1"
                     /translation="MIAAPVRVAVTNDFELVVAGLARMLEPFAERVSVVAQPVRGAPV
                     PSPVDVALFDTFGHPNSGYDEILRLRADPNVERVAVFTWDFDPKFLDRALGMGVTGYL
                     GKNLSAAELVDALERVAAGERVVTTNPATDRHAPPGRSWPGRETGLSERESEVLILIA
                     EGLSNVEIATALHISVNSVKSHVRNGYRKLGVRTRAQAVRTVLERSMIRRSMTPTDAD
                     GG"
     gene            complement(2130..>2553)
                     /locus_tag="VIX62_08425"
     CDS             complement(2130..>2553)
                     /locus_tag="VIX62_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860294.1"
                     /translation="VTFIVMLVVAFAIGFRFEGGVWDGLMATLLLLYFSYAFSWIQAL
                     VGLSVKSVEAANSAGFMWMFPLTFLSSAFVDTSSMPPWLQTIADLNPFTVATNAARAL
                     YNGYDPGGAVWQALAWATGLIIVFSYLAMRRFAGTASR"
BASE COUNT          411 a          844 c          895 g          403 t
ORIGIN      
        1 ccgtcgacga tggtcttcga cgaccacgac atgatcgatg actggaacat ctccgaggcc
       61 tgggtgcagg acatccgcag agaaccgtgg tggccggagc acatcatcgg tggcctcatg
      121 tcgtactgga tctatcagca cctcgggaac ctcagcccgg acgagatcga cgccgaaggt
      181 ttgctggcac gtgtgcagca ggccgacgac gcgcgagacg tgctcagggc ctgggccttc
      241 gcctccgatg aatgcacgcc actgcccggc ggataccggt tcagctgcta ccgagagctc
      301 ggcgcggtcc ggctcgtcgt gatcgaggcc cggaacgccc gcacgctcga ccgcggcaag
      361 cgggcgatgg tgggcgacga cgaatgggcg tgggtggcgg agctctgccg gcgcccggtg
      421 ggtcatctcg tcattgcgac gtcactcccg gttttcgtgc ccggtgggct ccacggcttg
      481 cagcagtgga acgaggcggt gtgcgacggc gcgtggggac ggccgttcgc gtggctcggc
      541 gagcggctcc ggcgggccct ggacctggag gattggcccg cgttcgatcg atcgttccgg
      601 gccttcgtcg ctctcctcgc ggaggtggtt gcgcccgggc ccggcgggtc gccgtccacg
      661 atcacggtgg tgtcgggcga catccacttc agctacgtcg ccggtatcca actggcgggc
      721 agcgtggagg gatcgacgcg catcacccag gtcgtcagct cgccgatccg caacgccctg
      781 accgggcgtg atcgtcgcgt gctgcagttc ggggtgtccc ggttcggtcg gtggatcggt
      841 ggatggctcc aacgccgcgt cggccggggt ccgagtgcgg cgacgtggga gctcgacgac
      901 ggaccgttct tcgacaactc gatgggcgaa ctcgtcttcg agggtgatgc tgcgaagctg
      961 ctcatcgagc gctcccgacc ggacgatgac ggagagccca ttctcgagat catggtcgac
     1021 caccggctct gatctcagga cgaggccagc caccgttcat ccagttcgca gatcgcgagg
     1081 acccggcgca cgatcggcag ctcgttgacg atgcgcaact gcgagcccga cggcagctcg
     1141 tgctcgaacg ccagcagcgc gctgacgcct gccgccgtca ccaggtcgac gcccgagaga
     1201 tccaggagca tcgtggccgg ttccctcggc gcgacggcct cggcccaggc ccggaactcc
     1261 gtgaccgtac cgatgtccag cacggcgaag gggacaggca acggcgccgc cgcgagagac
     1321 gttgatccaa ccacggctcg attttccgcg attcgggccc cccgccgcct cacccctagg
     1381 ggtattgtgc gcagcgctcg gaccggtcac gctgtagccg atgatcgctg caccggtgcg
     1441 tgtcgccgtg accaacgact tcgagttggt cgtcgccggc ctggctcgga tgctggagcc
     1501 gttcgccgaa cgggtgtcgg tcgtcgctca acccgtccgg ggggcgccgg tcccgagccc
     1561 tgtcgacgtg gcgttgttcg acacgttcgg ccacccgaac agcggctacg acgagatcct
     1621 ccggctgcgg gccgacccga acgtggagcg cgtcgccgtg ttcacctggg atttcgatcc
     1681 gaagttcctt gatcgagcgc tcgggatggg ggtcacgggc tacctgggga agaacctcag
     1741 cgccgccgag ctcgtcgatg cgctcgagcg ggtagcagct ggcgagcggg tggtcacgac
     1801 gaatccggcc accgaccgcc atgcgccgcc gggcaggagc tggcccggtc gggagacggg
     1861 tctctccgag cgggagagcg aggtgctgat cctgatcgcc gagggtctca gcaacgtgga
     1921 gatcgcaacc gcgctgcaca tctcggtgaa ctcggtgaag tcccacgttc gcaacggcta
     1981 ccggaagctc ggcgtgcgta cgcgcgccca ggccgtccgg accgtgctcg agcgttccat
     2041 gatccgccgc tcgatgacgc cgaccgacgc tgatggcggc tgacgctgcc gcccgactcg
     2101 atttcaacgg ctggtgccgc tcacccagct caacggctgg cggtgcccgc gaagcgacgc
     2161 atcgccaggt acgagaagac gatgatcagc cccgtcgccc acgcgagcgc ctgccagacg
     2221 gcgccgcccg ggtcgtaccc gttgtagagc gcgcgggctg cgttcgtggc caccgtgaac
     2281 gggttgaggt cggcgattgt ctgcagccac ggcggcatgc tggatgtgtc gacgaacgcc
     2341 gacgagagga acgtgagcgg gaacatccac atgaagccgg ccgagttggc ggcctcgacc
     2401 gacttgaccg agagcccgac gagcgcctgg atccagctga acgcgtagct gaagtagagc
     2461 agcagcagcg tggccatgag cccgtcccac acgccgccct cgaagcggaa gccgatcgcg
     2521 aacgcgacga cgagcatcac gatgaacgtg acg
//
LOCUS       DATDBZ010000348         2835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_739608, whole genome shotgun sequence.
ACCESSION   DATDBZ010000348 DATDBZ010000000
VERSION     DATDBZ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739608"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VIX62_08430"
     CDS             complement(<1..455)
                     /locus_tag="VIX62_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860295.1"
                     /translation="MSPIRALIVDDHPVTREGLRTALELSDEVVVVVGEAASGEEAVE
                     RARELTPDVVFMDVRMPGMDGIEATRRIRQAAPTTKIILITIDESRGAISDAIQAGVS
                     GYLLKDASPDALVDAAKNAIEGNAVIHPQLTKTFIEEVRLGDSDAPKAAP"
     gene            549..953
                     /locus_tag="VIX62_08435"
     CDS             549..953
                     /locus_tag="VIX62_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014124816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEY5860296.1"
                     /translation="MQEEIATETDTQATEAPEAETTQNAGAASTDEAGSSTKDAILEA
                     LKNVIDPELGLNIVDLGLVYDVQLDAQGDVDIQYSLTTMGCPIGPMIEDQMRGFLRGV
                     PGVGEVRPEFVIRPPWTPEMMSDEAKASLGIF"
     gene            1256..2101
                     /locus_tag="VIX62_08440"
     CDS             1256..2101
                     /locus_tag="VIX62_08440"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY5860297.1"
                     /translation="MADYAKPEVLVSTDWLEDHLDDPNVRIIEVDEDTASYDKGHVRN
                     ALKWDWSTDLHTEVGRDFQDQAGFSRLLSEAGVGPDTTVVLYGGNNNWFAAYAYWLLK
                     LRGYDDVKLVNGGRKKWELESRELVQEVPSFGATGFTISGAERPELRALRDEVIGKVG
                     AVGFVDVRSPEEYRGEKLAPDHLPQEQSQVPGHIAGAANIPWAKAANDDGTFRTADEL
                     KELYADEGITGDGEIIAYCRIGERSSHTWFALQELLGYENVKNYDGSWTEYGSLVGAP
                     VEIGG"
     gene            complement(2116..>2835)
                     /locus_tag="VIX62_08445"
     CDS             complement(2116..>2835)
                     /locus_tag="VIX62_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5860298.1"
                     /translation="GGQDDPHRGVDRCRRAAVPMGPDRLVRRRSDGIAAVAGFAVLVT
                     CGLIARSGTVGDAERSVFEAINGLPEGLEPVMHQAQFLGVLAIGPVVALIALALHRYR
                     LAAAAALVTVFKLVGERIVWKVVDRERPGVTEPNAIVRGGTDTEGVSFVSGHVVLVTG
                     LAWVITPYLRGRWRIIPWLVVALVCFARIYLGAHNPLDVLGGFALGVAIGAIANLLVK
                     VPAAGADTDEPGREAPAPSSG"
BASE COUNT          489 a          942 c          991 g          413 t
ORIGIN      
        1 ggcgcggcct tcggtgcatc ggaatcgccc aggcggacct cctcgatgaa ggtcttcgtg
       61 agctgggggt ggatcacggc gttgccctcg atcgcgttct tcgccgcgtc gaccagcgcg
      121 tccggcgagg cgtccttcaa caggtagccc gagacgccgg cctggatcgc atcggagatg
      181 gcgccgcggg actcgtcgat cgtgatgagg atgatcttgg tggtgggagc ggcctggcgg
      241 atgcgccgcg tggcctcgat gccgtccatc ccgggcatgc ggacgtccat gaacaccacg
      301 tcaggagtca gctcgcgggc gcgttccacg gcttcctctc cgctggcggc ctcgcccacc
      361 acgaccacga cctcgtcgga gagttcgagt gcggtgcgga gaccttcgcg ggtgaccgga
      421 tggtcgtcca cgatgagggc tcggatcgga gacatcgccg ggctgctcct tccgttcggg
      481 gaagtattcc gtttggggac gtacgatgga cgctgtaagg ataggacgac tcgaggcggg
      541 aagcgcacat gcaggaagaa atcgcaaccg agaccgacac gcaggcgacc gaggctccgg
      601 aggccgagac cacccagaac gccggtgcgg caagcaccga cgaggcaggg tcctcgacca
      661 aggacgcgat cctcgaggcc ctgaagaacg tcatcgaccc cgagctggga ctgaacatcg
      721 tcgacctcgg gctggtgtac gacgtgcaac tggatgccca aggcgacgtc gacatccagt
      781 actcgctcac cacgatggga tgcccgatcg gtcccatgat cgaagaccag atgcggggct
      841 tcctgcgcgg tgtgcccggg gtgggagagg tccgtcccga gttcgtgatc cggccgccgt
      901 ggacccccga gatgatgtcg gacgaggcca aggcgtcgct cggcatcttc tgatccgcgc
      961 cgccgcgagg ggctcgcctt gacgctggct ggtccctgcg gtacgtattg cacctgcaat
     1021 tccgaccgga ggaccgtgat ttgatccgta cgaggtgcat cggggtggtg ggggcgatga
     1081 tcccgtctcg cctgtgttgt atgccccagc gcgcccaccg gggggcgccg atgcatctgc
     1141 ggccatagct ccaccgcccg gaacccatcg ctccgatcac cgccgacggc cgcatctgcg
     1201 gccgttcatc gtttcccggc tccgtggggg ccggcgcacc ggaaggagaa cgttcatggc
     1261 cgactacgcg aagccggagg tgctcgtcag caccgactgg ctcgaagacc acctcgacga
     1321 tccgaacgtc cggatcatcg aggtcgacga agacacggcg tcctacgaca aggggcacgt
     1381 gcgcaacgcc ctgaagtggg actggagcac cgatctccac acggaggtcg ggcgcgattt
     1441 ccaggaccag gccgggttct cgcgcctgct gtccgaggcc ggagtgggac cggacaccac
     1501 cgtcgtcctc tacggcggca acaacaactg gttcgccgcc tacgcctact ggctgttgaa
     1561 gttgcggggg tacgacgacg tgaagctggt caacggtggg cgcaagaagt gggagctgga
     1621 gagccgcgag ctcgtccagg aggtgcccag cttcggggcc acggggttca cgatcagcgg
     1681 tgccgagcgt cccgagcttc gagccctgcg cgacgaggtg atcggcaagg tgggcgcggt
     1741 gggcttcgtc gacgtccgct cgcccgagga gtaccggggc gagaagctgg cgcccgacca
     1801 cctgccgcag gagcagtcgc aggtgcccgg ccacatcgcc ggcgccgcga acatcccctg
     1861 ggcgaaggct gccaacgacg acggcacctt caggacggcg gacgagctca aagagctcta
     1921 cgccgacgag ggcatcaccg gtgacggcga gatcatcgcg tactgccgga tcggtgagcg
     1981 ttcgagccac acgtggttcg ccctgcagga gctcctgggc tacgagaacg tcaagaacta
     2041 cgacggctcc tggaccgaat acggatcgct cgtcggcgcg ccggtggaga tcggcggcta
     2101 gggacccccg tccgttcagc cgctcgacgg agccggggcc tcgcgccccg gctcgtcggt
     2161 gtcggccccc gccgcgggaa ccttcacgag caggttggcg atggccccga tcgccacgcc
     2221 cagggcgaac ccgcccagga catcgagtgg attgtgggcg cccaggtata tccgggcgaa
     2281 gcagacgagg gccacgacga gccacgggat gatgcgccag cggcctcgca ggtacggcgt
     2341 gatcacccac gcgagcccgg tcaccagcac gacgtggccg gagacgaacg agacgccctc
     2401 ggtgtcggtg cctccgcgga cgatcgcgtt cggctcggtg acgccggggc gctcccggtc
     2461 gacgaccttc cacacgatgc gttcacccac gagcttgaag accgtcacga gcgcagcggc
     2521 cgccgccaat cggtaccggt ggagggcgag cgcgatcagg gcgacgaccg gcccgatcgc
     2581 caggaccccg aggaactgcg cctggtgcat caccggctcg aggccctcgg gcaagccgtt
     2641 gatcgcctcg aagaccgagc gttccgcgtc gcccacggtg ccgctgcggg cgatgagccc
     2701 gcacgtgacc agcacggcga agcccgcgac ggcggcgatc ccgtccgagc gacgccgaac
     2761 taggcggtca ggccccatcg gtacagccgc acggcgacat cgatcgacac cacggtgagg
     2821 gtcatcctga ccgcc
//
LOCUS       DATDBZ010000349         4590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_739655, whole genome shotgun sequence.
ACCESSION   DATDBZ010000349 DATDBZ010000000
VERSION     DATDBZ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739655"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="VIX62_08450"
     CDS             <1..1203
                     /locus_tag="VIX62_08450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5860299.1"
                     /translation="SMTVTRRQVLAGGAVAAAGLALRPVRSLAVGAPAEQLAPPSGLE
                     SFVFDRMRAGRLPGVSAAIVRDGQLWARGFGEANLARDRHVDSGTLFMLASISKTVIC
                     TAVMQAVEDGLFSLDDDVDDVLGFSVRIPGFPNRKITPRQLLTHTSSIRDRWPVWDDL
                     YSEGDSPIGLGEFLEGYLVPGGEDYREGNFYGFAPGKDYRYSNVGASLAAYLVEAASG
                     IGFDEWCNDRIFEPLAMTRAGWHLADVTRREVAMPYRWSPDLDKYVPYGQYGYPDYPD
                     GALRTTARHLCHHMGMVMNGGEWNGVRILSAESVQAIRRSQVPGIESGQGLIWYRFGF
                     HGRSLVGHNGGDQGVATVAFFEPDTGIGVAVLGNGNWRKDGNRWPLQEIMQRLFEDAP
                     QLLEIGTS"
     gene            1200..1961
                     /locus_tag="VIX62_08455"
     CDS             1200..1961
                     /locus_tag="VIX62_08455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5860300.1"
                     /translation="MNEAAQPALYSELAPWFHLLTSPDEYVDEAAQAISLFTEVLGEP
                     PGTILELGSGGGNNASHMKAHAALTLTDLSPEMLKLSRSLNPECEHIQGDMTTLRLGR
                     TFDGVFVHDAVSYLTTEEQLRAAIETAFVHTRPGGAAIFCPDHVRERFREWTDHGGHD
                     GRDGDPRSLRYLEWTWDPDPDDTWFVDDFAYLLREGPGEPRVVHDRHVLGLFPRATWL
                     SLFTHVGFTPQIRTSTWIEDGDEAGAEIFLASRPL"
     gene            2022..2285
                     /locus_tag="VIX62_08460"
     CDS             2022..2285
                     /locus_tag="VIX62_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5860301.1"
                     /translation="MPVVTLRAPLKDRAGKVGQHDLPGATVGEVLRELERRYPTVKGW
                     ILDDQGQVRAHVNVFVNGERTRGDTAVGDTDVLQVLPSIAGGS"
     gene            2293..3372
                     /locus_tag="VIX62_08465"
     CDS             2293..3372
                     /locus_tag="VIX62_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEY5860302.1"
                     /translation="MPDTELLVGTKKGLFVLRGDPSRGEPFDVATRAFAGDVVEFAMR
                     DPRTGRYFASVTSGFYGPRVFFIDGDPTGEWTMSEGPVMPEETETSVERIWMIKPGEA
                     PGQIWAGVAHAALFESRDDGVTWTLNDALWKERVAGDWQPGAGGLALHSICPWPGDPQ
                     RMAIGVSAAGVWITEDGGKSWRTGYAGMVPAYIPEDQTEDTNVLCVHNMHRAPTQPER
                     LFMQFHGSVYRSDDEGGSWNDIHEGLPSGFGFPLAVDPADPDSAFVIPLNADVDRVTP
                     DGAVRVFETRDAGASWIERSKGLPGPEAYLTIYRQAFGTSGQGDELQLYFGATSGDVF
                     GSADAGASWFSAHERLAPVTSVRVA"
     gene            complement(3264..4019)
                     /locus_tag="VIX62_08470"
     CDS             complement(3264..4019)
                     /locus_tag="VIX62_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860303.1"
                     /translation="MVIALTPGAGQAGGSWIEFDGPLVVGTLVTGSGTFGNGQQAGVG
                     AGPWFATLRSEEPGVDDIALGPVEVSRASSFGWRATVTFVVPDVPTGEYWVDIANDDG
                     EGVGDLIGGFVVISSTPLEGRLWSRVSTAEAGRANANRSADRLRDRQGQLEEALSDRQ
                     ATIERQAERLTAAGARITRLGSDLDEATASRSTWPFWLAALMAVALAFAGFVLGRRYA
                     TRTLVTGASRSWAENQEAPASAEPNTSPLVAPK"
     gene            4165..4416
                     /locus_tag="VIX62_08475"
     CDS             4165..4416
                     /locus_tag="VIX62_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860304.1"
                     /translation="MAQVIELADYRRRRNPLVAAMQRLERAVSRLDPLVRDRTGRLGP
                     AVERELAHIARSVSSGSPQDAAERAERLADLLEHPAANG"
BASE COUNT          695 a         1586 c         1579 g          730 t
ORIGIN      
        1 tcgatgacgg tgacgcggcg gcaggtgttg gccggcggtg cggtggccgc ggcgggcctg
       61 gcgctgcggc ccgtgcgatc gctggcggtc ggcgcccctg cggagcagct ggctcctccg
      121 agcggcctgg aatccttcgt cttcgatcgc atgcgggccg gtcgactacc gggcgtctca
      181 gccgcgatcg tgcgagacgg gcagttgtgg gcgcgaggct tcggcgaggc gaacctggca
      241 cgagaccgcc acgtcgacag cggcacgttg ttcatgctgg cgtcgatctc caagacggtg
      301 atctgcaccg cggtgatgca ggcggtcgag gatggattgt tctccctgga cgacgacgtg
      361 gacgacgtgc tgggcttctc ggtgcggatc cccgggttcc ccaatcgcaa gatcacgccg
      421 cgtcagttgc tgacgcacac ctcgtcgatc cgcgaccggt ggccggtctg ggacgacctg
      481 tactccgagg gcgattcgcc gatcggactc ggcgagttcc tggaggggta cctggtgccc
      541 ggcggcgagg actatcgcga gggcaacttc tacgggttcg caccggggaa ggactatcgc
      601 tactccaacg tgggggcgtc gttggcggcc tacctggtgg aggcggcgag tggcatcggc
      661 ttcgacgagt ggtgcaacga tcggatcttc gagccgctgg cgatgacccg agcgggctgg
      721 cacctggcgg acgtgacgcg ccgtgaggtt gccatgccgt atcgctggtc ccccgatctc
      781 gataagtacg tcccgtacgg tcagtacggc taccccgatt accccgacgg cgcgctgcgc
      841 accacggcgc gccatctctg ccaccacatg ggcatggtca tgaacggcgg cgagtggaac
      901 ggcgtgcgca tcctttcggc cgagtcggtc caggcgatcc gccgctcaca ggtccccggc
      961 atcgaaagcg ggcagggcct gatctggtac cggttcggtt tccacggccg ctcgctggtc
     1021 ggccacaacg gcggtgatca gggcgtggcg accgtcgcgt tcttcgagcc cgacaccggc
     1081 atcggcgtcg cagtcctcgg caacggcaac tggcgcaagg acggcaaccg ctggcccctc
     1141 caagagatca tgcagcgcct gttcgaggac gctccccagc tgctcgagat tggaacgtcg
     1201 tgaacgaagc tgcacagcct gcgctctact ccgagctcgc gccctggttc catctgctga
     1261 cctcgccgga tgaatatgtc gacgaggccg cgcaggcgat atcgctgttc accgaggtgc
     1321 tcggcgaacc accgggcacg atcttggaac tcggcagcgg gggcgggaac aacgcctcgc
     1381 acatgaaggc gcacgcggcg ctcacgctca ccgacctgtc cccggagatg ctgaagctca
     1441 gccggtcgct caacccggag tgcgaacaca tccaggggga catgacgacc ctgcgactgg
     1501 gtcgcacctt tgacggcgtg ttcgtgcacg acgccgtgtc ctacctcacg accgaggagc
     1561 aacttcgcgc cgcgatcgag accgcgttcg tgcacacgcg ccccggaggg gcagccatct
     1621 tctgtcccga tcatgtgcgt gaacggttcc gcgaatggac cgatcacggt ggccacgacg
     1681 gcagggacgg cgatcctcgc tcgctgcgat acctggagtg gacctgggat ccggaccccg
     1741 acgacacctg gttcgtggac gacttcgcct acctgctccg cgagggcccc ggcgaacccc
     1801 gggtggtgca cgaccgccac gtgctcggcc tgttcccgcg ggccacctgg ttgtcgctgt
     1861 tcacgcacgt cggcttcacc ccgcagatcc ggacctccac ctggatcgag gatggcgacg
     1921 aggcgggtgc cgagattttc ctggcgtccc gacccctctg aacctggcga agcaacatct
     1981 cgcaacccgg gccgtcggcg ggccgcgcta ggctgccgcc catgccggtg gtcacgcttc
     2041 gagcgccgct gaaggatcgc gcgggcaagg tcggccagca cgacctgccg ggtgccaccg
     2101 tgggtgaggt cctgcgcgag ctcgagcgcc gctaccccac cgtgaagggc tggatcctgg
     2161 acgatcaggg ccaggtccgg gcccacgtca acgtgttcgt caatggcgaa cggacgcgcg
     2221 gggacacggc cgtcggcgac accgacgtgc tgcaggtcct gccgtccatc gccggaggga
     2281 gctgaagcac gcatgccaga caccgaactc ttggtcggca cgaagaaggg gctgttcgtc
     2341 ctgcgcggcg acccttcgcg cggtgaaccg ttcgacgtgg ccacacgcgc gttcgccggg
     2401 gacgtggtcg agttcgccat gcgcgaccct cgcaccggcc gatacttcgc cagcgtcacc
     2461 tcggggttct acggaccgcg cgtgttcttc atcgacggcg accccaccgg cgagtggacc
     2521 atgagcgagg gtcccgtgat gccggaggag accgagacgt cggtcgagcg catctggatg
     2581 atcaagcctg gcgaggcgcc ggggcagatc tgggcgggcg tcgcccacgc ggcgctgttc
     2641 gagagccgcg atgacggtgt cacctggacg ctgaacgacg ccctgtggaa ggaacgcgtg
     2701 gcgggcgact ggcagcccgg tgccggcggc ctggcgctgc actcgatctg cccgtggccc
     2761 ggcgatcctc aacggatggc catcggcgtg agcgccgccg gcgtgtggat caccgaggac
     2821 ggcggcaagt cgtggcgcac cggctacgcc ggcatggtgc ccgcgtacat ccccgaggat
     2881 cagaccgagg acacgaacgt gctgtgcgtg cacaacatgc atcgcgcacc cacgcagccg
     2941 gagcggctgt tcatgcagtt ccacggcagc gtgtaccgca gcgacgacga aggtggcagc
     3001 tggaacgaca tccacgaagg cttgcccagc gggttcgggt tcccgttggc cgtcgatccc
     3061 gccgatccgg acagcgcctt cgtgatcccg ctgaacgccg acgtcgaccg cgtcacgccc
     3121 gacggcgcgg ttcgggtgtt cgaaacacgc gacgcgggtg cttcctggat cgagcgatcg
     3181 aagggactcc cgggaccgga ggcctacctc acgatctacc ggcaggcgtt cggcacgagc
     3241 ggccagggcg acgagctgca gctctacttc ggcgccacca gtggcgacgt gttcggctcg
     3301 gccgatgccg gtgcctcctg gttctcggcc cacgagcggc tggcgccggt caccagcgtg
     3361 cgggtcgcgt agcggcgtcc cagcacgaac ccggcgaagg ccagcgccac cgccatgagt
     3421 gcggcgagcc agaacggcca cgtcgacctc gacgctgtcg cttcgtcgag gtcggatccc
     3481 agacgggtga tccgcgcccc ggcagcggtg agacgttcgg cctgacgctc gatcgttgcc
     3541 tgccgatcgc tcaacgcctc ttccagttgg ccctgtcgat cccgcaaccg gtcggctgat
     3601 cggttggcgt tcgcccgccc cgcctccgcc gtactcaccc gcgaccagag tcggccttcg
     3661 aggggcgtgc tggagatcac gacgaaccca ccgatgaggt cgccgacgcc ctccccgtcg
     3721 tcgttggcga tgtcgaccca gtactccccc gtcgggacgt cgggcacgac gaaggtcacc
     3781 gtagccctcc agccgaagga cgaagctcgg ctgacctcga cggggcctag ggcgatgtcg
     3841 tcgaccccgg gctcctccga tcgcaaggtg gcgaaccacg gaccggctcc gacgcctgcc
     3901 tgctgaccgt tgccgaaggt gccactgccc gtgacaaggg tgccgacgac gagcgggccg
     3961 tcgaactcga tccatgaccc tccggcctgc cccgcaccgg gtgtcagcgc gatgaccatc
     4021 gtggcgaacg tcagcgtgat caggagcttg cggatgtcga ctccccccgt acgagcggtc
     4081 ccatgggggt gagatgccgc ggcccgcccc cgcgttgcaa cggatgcgct tggcggtgtc
     4141 agaccggggg gtcatgatgt cgtcatggca caggtgatcg aactcgctga ctaccggcgg
     4201 cgccggaacc ccttggtggc tgcaatgcaa cggctcgaac gcgccgtctc gcgcctggat
     4261 ccgctggtgc gcgatcgcac cgggcgcctg gggcccgccg tcgagcgcga actcgcgcat
     4321 atcgcgcggt cggtctcctc cggctcgccg caggatgccg ctgagcgcgc cgagcgcctc
     4381 gccgatctcc tggagcaccc agcggccaac ggctgagttc ggtttccacg ggtaccatcg
     4441 accggtgctg aagccttccc tccggcgcgc ccgcggcgcc gcggcactgg ccgtcgcgct
     4501 cgggtgtctg ttcatcgcct cccctccgac cggcgccgcc gtgcagcctg cgggttccac
     4561 gtggctgcct cccgcctacc tcatgtggcg
//
LOCUS       DATDBZ010000350         2423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135 k141_98128,
            whole genome shotgun sequence.
ACCESSION   DATDBZ010000350 DATDBZ010000000
VERSION     DATDBZ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98128"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VIX62_08480"
     CDS             <1..196
                     /locus_tag="VIX62_08480"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5860305.1"
                     /translation="VIGFVPGFQVAWQAHLGGLAIGALVGLIYARTRSVRRRALQVWL
                     LVAVAAGLVALLLVPPLIYG"
     gene            complement(353..592)
                     /locus_tag="VIX62_08485"
     CDS             complement(353..592)
                     /locus_tag="VIX62_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEY5860306.1"
                     /translation="MARPGKDDDSLVERTEGEAAPNPVWFKPIMIGLMLVGLVWVLVF
                     YLSGSNFPIPGIGAWNLVIGFGIAFVGFLMTTRWR"
     gene            702..1502
                     /locus_tag="VIX62_08490"
     CDS             702..1502
                     /locus_tag="VIX62_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEY5860307.1"
                     /translation="MDEATIESADKTRTPTRRRITVTGVLGDLLLTAGVITLLYVGWQ
                     LWIGDLIYGAQRNATGQELSEEWEQEYAAPAPSEAASDPVPSDAPSPEAPVAVEPVVL
                     PEPADAEVFGIMHIPRFGADYQVPMAGGVSRPRTLDPIGIGHYPGTMMPGEVGNFAVA
                     AHRTTYGKPFNRIAELHINDAIIVETANGWYAYRFRTLEYVTPDEVQVLLPVPQVEDA
                     PPGSRYITLTSCSPMFAMSERIVAYGVFESFTPRAAGAPAALTDGVAA"
     gene            1502..1657
                     /locus_tag="VIX62_08495"
     CDS             1502..1657
                     /locus_tag="VIX62_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860308.1"
                     /translation="MYAALWHALPGPWWVRVLIIVVLIVAVLYGLFVFGFPWISQLVN
                     PQEVTVE"
     gene            1679..2317
                     /locus_tag="VIX62_08500"
     CDS             1679..2317
                     /locus_tag="VIX62_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181696.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY5860309.1"
                     /translation="MIGQAPILVVDNHDSFVHTLVGYLHELGADTDLVEADAIDTDAA
                     SSVLAGYRGVLISPGPGTPARAGASIAVVRAAAAAGTPLLGVCLGHQAIAEAYGATVS
                     HAPELMHGMTSLVEHDGGALYEGLPNPFTATRYHSLAIVPGTVPAELVVTSSTDSGVI
                     MGIAHRTSPIQGVQFHPEAVLTEGGHRLLGNWLETVGFADAAARGAALHPRR"
BASE COUNT          368 a          835 c          825 g          395 t
ORIGIN      
        1 cgtgatcggc ttcgtgcccg ggttccaagt cgcctggcag gcgcacctcg gcgggctggc
       61 gatcggtgcg ctggtgggtc tgatctatgc gcggacgcga tcggtgcggc ggcgggcgct
      121 ccaggtgtgg cttctggtcg cggttgcggc cggactggtc gccctgctgc tggtgccgcc
      181 gctgatctac gggtgacctg gatcatctag gtatcgatat gcgcgcttcg cgcgctcctc
      241 gaccttgccc cgtgcgctca cgcgcaagtt atccacagat ctatccacac ctggggatga
      301 atcacactcg tgtaattcgg cgcggcggga accgcggacg acgagaggag cgtcagcgcc
      361 agcgggtcgt catcaagaag ccgacgaagg cgatgccgaa gccgatgacg aggttccatg
      421 ccccgatgcc ggggatcggg aagttgctgc cgctgaggta gaacaccagc acccagacga
      481 gtccgaccag catgaggccg atcatgatcg gcttgaacca caccggattc ggtgccgcct
      541 cgccttcggt gcgctccacc aaggagtcgt cgtctttgcc gggtcgtgcc atacgtcaca
      601 tcctacctct cagccgtacc cctgccaggc ggcccccgcg tcgcacccgg cgggccgccc
      661 ccggcagcgg ggccacagcc cgtgcgaact agaatccacg tgtggacgag gcgacgatcg
      721 agagtgcgga caagacccga actcccacgc gtcggcgcat cacggtgacc ggagtactgg
      781 gcgacctgct tctcactgcg ggcgtgatca ccctcctcta cgtcgggtgg cagctgtgga
      841 tcggagacct gatctacggt gctcagcgca atgccaccgg gcaggagctg tcggaggaat
      901 gggagcagga gtacgcggct ccggcgccga gcgaggcagc gagcgacccg gtgccgagcg
      961 acgcgccctc gccggaggcg ccggtcgcag tcgagccggt cgtcctgccc gaaccggccg
     1021 atgccgaggt gttcggcatc atgcacatcc ctcgcttcgg cgccgactat caggtgccca
     1081 tggccggcgg cgtcagccgc ccacgcacgc tcgaccccat cggcatcggg cactatcccg
     1141 gcacgatgat gcccggcgag gtcggcaatt tcgccgtcgc cgcgcaccgc acgacctacg
     1201 gcaagccgtt caaccgcatc gccgagctgc acatcaacga cgcgatcatc gtcgagacgg
     1261 ccaacggctg gtatgcctac cgcttccgta cgctcgagta cgtgactccc gacgaggtgc
     1321 aggtgcttct gccggtacct caggtcgagg acgccccgcc cggctcccgc tacatcacgc
     1381 tgaccagctg cagtccgatg ttcgcgatga gcgagcgcat cgtcgcgtac ggcgtgttcg
     1441 agtcgttcac cccgcgggcg gccggagcgc ctgccgcgct gacggacgga gtggccgcct
     1501 gatgtacgcc gcactgtggc acgcccttcc cggcccgtgg tgggtgcggg tgctgatcat
     1561 cgtcgtactg atcgtcgcgg tcctttacgg gctgttcgtc ttcggattcc cgtggatcag
     1621 tcagctcgtg aacccgcagg aagtcaccgt tgagtaaggc tccgtcggag cgggtgcgat
     1681 gatcggccag gcaccgatcc tggtcgtcga caaccacgac agcttcgtgc acacactcgt
     1741 cggctacctg cacgagctgg gcgccgacac cgacctcgtc gaggctgacg cgatcgatac
     1801 ggatgctgcg tcgtccgttc tcgccggcta ccggggtgtg ctcatctcac ccggaccggg
     1861 gacgcccgcg cgcgccggcg catcgatcgc cgtggtgagg gcggccgcgg ccgccggaac
     1921 ccctctgctc ggcgtctgcc tgggccacca ggccatcgct gaggcctacg gcgcgaccgt
     1981 gtcgcacgca cccgagctga tgcacgggat gacctcgctc gtcgagcacg acggcggtgc
     2041 gctgtacgag gggcttccca atccgttcac ggccacccgc taccactcgc tcgcgatcgt
     2101 gccgggcacg gttcccgccg agctggtggt gacgagctcc accgacagtg gggtcatcat
     2161 ggggatcgcg caccgcacgt caccgatcca gggcgtgcag ttccaccccg aggcggtcct
     2221 caccgagggt ggacaccgcc tgctgggcaa ttggctcgaa acggtcggtt tcgcggatgc
     2281 cgcggcgcgc ggcgccgcgc tccacccgcg tcgctgagac ggcgagccgg tgccgcgggg
     2341 tcggcgaggc ggcaggtccc gggccggtgg ggctacggtc cggagaagta ggtgaggacg
     2401 acctcggcgt ggatcggcgc ctc
//
LOCUS       DATDBZ010000351         3967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1340865, whole genome shotgun sequence.
ACCESSION   DATDBZ010000351 DATDBZ010000000
VERSION     DATDBZ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340865"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VIX62_08505"
     CDS             <1..838
                     /locus_tag="VIX62_08505"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY5860310.1"
                     /translation="QGLSVTQFEITTLGRGSSDLTAVAMAAAVGADVCEIYTDVQGVF
                     TTDPRRVPNARKLEVVSYDEMLEMAAAGANVLQSRSVEYARRHGVKLHVRSAFDDAPG
                     TWVQEVADMEGVLISGVALDTGEAKVTLERVPDRPGVAALVFKAIAEDAISIDMIVQN
                     ISHAGATDLSFTAPLTDIARLQSVMERIVKEIGAERFTMDQDIAKISLIGAGMKTHPG
                     VAADMFDCLATGGVNIEMISTSPIRISCVIRRGDGERAVRLIHERFDLDATGSSPDIA
                     TG"
     gene            878..1903
                     /locus_tag="VIX62_08510"
     CDS             878..1903
                     /locus_tag="VIX62_08510"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY5860311.1"
                     /translation="MKIAVVGATGAVGREMMRTLEERRVPVDGLLPLASSRSAGTTVP
                     FRGESYEVRELSLDLLRGVDVALVSAGASVSRSFIPEAAANGTVCIDNSSAFRMDPDV
                     PLTIPEVNPESLEGSPRIIAVPNCTTITALLAVAPLHRAAGLRSLVLSSYQSVSGAGH
                     TGVSELLDQVEKLRGDEASLAHPVHGALPSGPVFGKTIAYNVVAKIGDFEADGFTGEE
                     SKMMAEPRKILGMPDLPVVATSVRVPVLAGHGASILAEFERSISVRDARELLEAAPGV
                     ELWDDPKKDVYPSPLDSAGLDVALVGRIRQVPGRPDALALFSCADNLRKGAALNAVQI
                     AEHLFAS"
     gene            1951..2319
                     /locus_tag="VIX62_08515"
     CDS             1951..2319
                     /locus_tag="VIX62_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEY5860312.1"
                     /translation="MSYQDFMEGTVKVFELVGVAVLLIGGLLAFSLYFFRLVSGVPRR
                     QAFDEVRSSLGRAILLGLEVLVVADIIRTIVVTPTLSSAATLGVIVLVRILLSFSIDV
                     EVDGILPWRRGAPRGEIPPS"
     gene            complement(2316..2792)
                     /locus_tag="VIX62_08520"
     CDS             complement(2316..2792)
                     /locus_tag="VIX62_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860313.1"
                     /translation="MVRLASPEDWPAVAGLLVELGRGVAAGTAEDPTHQQAFSGHIRQ
                     IGNVTLVAELDGEIAGVIDMEYHQRLGDHRPQARVNDVVVTEQARGSGVGTTLLRRAE
                     ELARKRGCFRMALVTASWRDATMDFYRREGWSDYGAWFVKQLADDVTPGGQPVDDN"
     gene            complement(2804..>3967)
                     /locus_tag="VIX62_08525"
     CDS             complement(2804..>3967)
                     /locus_tag="VIX62_08525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860314.1"
                     /translation="AHREQIAGVLSTSINFPLERALLNAPLLKLDDIRVALEDDRVVA
                     TAGEFRFDQWFGGRGIACSGITRVGTLPERRADGLATACTDALLQRAHERGAPLAALF
                     PAVLRPYRRLGFEIAGSYTTHRLPLDAIAPDRDSLSVELVDVDRDLAAIREAYRGWVR
                     NQNGPVEPVEDEHWRVRILTKPDDSAYRAVVVRQADRVTGFAAFSRDADPGPLDVAFG
                     LSCNAFFTTTPVAQRALLQYFRGFRGLGKWVEWSGPPSDPLALATPEAFLATPFRYDW
                     MLRLLNVPAALEARGYPPIDVDAVMAVEDERWPENAGPWRIEVRGGSASVSPAVGSDH
                     PAPIGIGTLSSLFSGFLRVHDAVRLGLLDADDPSLEALGAMFAGPDPWCPFFF"
BASE COUNT          607 a         1304 c         1416 g          640 t
ORIGIN      
        1 ccagggattg tcggtgaccc agttcgagat cacgacgctg ggtcgcggta gttcggacct
       61 gacggcggtc gccatggccg cggcggtggg cgccgacgtg tgtgagatct acacggatgt
      121 gcagggcgtc ttcaccaccg atccacggcg ggtgccgaac gcccgcaagc tcgaggtggt
      181 ctcctacgac gagatgctcg agatggccgc ggcgggcgcg aacgtgctgc agtcgcgctc
      241 ggtggagtac gcgcgtcgcc acggcgtgaa gctgcacgtc cgctcggcgt tcgacgacgc
      301 acccggcacc tgggtgcagg aggttgcaga catggaaggc gtgctgatct cgggtgtggc
      361 gctggacacg ggcgaggcga aggtgacgct cgaacgggtg cccgaccgcc ccggggtggc
      421 ggcgctcgtg ttcaaggcga tcgcggagga cgcgatcagc atcgacatga tcgtgcagaa
      481 catctcgcac gccggcgcga cggacctgtc gttcacggcc ccgctcaccg acatcgcccg
      541 tctgcagagc gtgatggagc gcatcgtcaa ggagatcggg gcggagcgat tcacgatgga
      601 ccaggacatc gcgaagatct cgttgatcgg tgccgggatg aagacccatc ccggtgtggc
      661 cgcggacatg ttcgactgcc tcgccaccgg tggcgtcaac atcgagatga tctcgacctc
      721 gccgatccgc atctcgtgcg tgatccgcag aggcgatggc gagcgggcgg ttcgcttgat
      781 ccacgaacgg ttcgacctcg atgccaccgg atcctcgccc gatatcgcca ccggttgacc
      841 gatcgggccg agggccgctg ttaggctgga acgcatcatg aagatcgctg tcgtcggtgc
      901 caccggggcc gtcggccggg agatgatgcg caccctcgag gagcgccgcg tccctgtcga
      961 cggcctgttg ccgctcgcgt cctcgcggtc cgcgggcacc acggtgccct tccgaggcga
     1021 gtcgtacgag gtgcgcgagc tgtctctgga tctcctgcgc ggggtcgatg tggcactcgt
     1081 cagcgccggg gcttccgtgt cgagatcgtt catccccgag gcggctgcga acggcacggt
     1141 gtgcatcgac aactccagcg cattccgcat ggatcccgac gtgccgttga cgatcccgga
     1201 ggtcaatccc gagtcgctcg agggatcgcc gcgcatcatc gcggtgccga actgcaccac
     1261 gatcacggcg ctgctcgcgg tagcgccgct gcaccgcgcc gccggtctca ggtcattggt
     1321 gctctcgtcc taccaatcgg tgagcggcgc cggtcacacg ggtgtcagcg aactgctcga
     1381 ccaggtggag aagctccgcg gcgacgaggc ctcgctcgcc catcccgtcc atggggcgct
     1441 gccgagcggt ccggtcttcg gcaagacgat cgcctacaac gtggtcgcca agatcggtga
     1501 tttcgaggcc gacggcttca cgggcgagga gtcgaagatg atggctgagc cgcgcaagat
     1561 cctggggatg cccgacctgc ccgtggtcgc caccagcgtt cgggtgccgg tgttggccgg
     1621 tcacggcgcg tccatcctgg cggagttcga gcggtcgatc tccgtgcgcg atgcgcgaga
     1681 gctgctcgag gccgcgcccg gcgtcgagct ctgggatgac ccgaagaagg acgtctaccc
     1741 ctcgcccttg gattcggccg gtctcgatgt ggcgctggtg gggcgcatcc gtcaggtgcc
     1801 cggccgccct gacgcgctcg cgctgttcag ctgcgccgac aacttgcgga agggcgcggc
     1861 cctcaacgcc gtccagatcg ccgagcacct gttcgcgtcg tgacctgagg ggtcgttggg
     1921 tcggggcatc ggcgggggta gggtcggccc gtgagctacc aggacttcat ggaaggcacg
     1981 gtcaaggtct tcgaactcgt gggcgtggcc gtgctgctga tcggtggcct cctggcgttc
     2041 tccctctact tcttccgatt ggtgagtgga gtgccgcgac ggcaagcctt cgatgaggtc
     2101 cggtcgtccc tcggccgcgc gatcctgctt gggctcgagg tgctggtggt ggccgacatc
     2161 atccgcacga tcgtggtgac gccgacgctg tcgagcgccg ccaccctggg tgtgatcgtg
     2221 ctggtgcgga tcctgctgag tttctcgatc gacgtcgagg tcgatggcat cctgccctgg
     2281 cgccgaggcg cacctcgagg cgagatcccg ccgagctagt tgtcgtccac gggctggccg
     2341 ccgggcgtga catcgtcggc cagctgcttc acgaaccacg ctccgtaatc gctccaacct
     2401 tcccgacggt agaagtccat cgtggcgtcg cgccacgagg cggtcaccag cgccatgcgg
     2461 aagcagccac gcttgcgggc gagttcctcg gcccgtcgaa gcaacgtggt tccgacgccg
     2521 gatcctcgag cctgttcggt gacgacgacg tcgttcacgc gggcctgcgg acggtgatcg
     2581 ccgaggcgct ggtggtactc catgtcgatg accccggcga tctcgccatc gagctcggcc
     2641 accagcgtca cgttgccgat ctgccggatg tgcccggaga aggcctgttg atgcgtgggg
     2701 tcctcggcgg tgccggccgc gacgccgcgg cccagttcga cgaggaggcc cgccacggcg
     2761 ggccagtcct cgggggacgc gagacgaacc acgaccgcac ccatcagaag aagaacgggc
     2821 accaggggtc ggggccggcg aacatcgcgc cgagcgcttc gagcgaggga tcgtcggcat
     2881 cgagcagccc gagccgaacc gcatcgtgca cacggaggaa cccgctgaac aacgacgaga
     2941 gcgtgccgat gccgatcggt gctgggtggt cgctcccgac cgccgggctc acgctcgccg
     3001 agccgccccg gacctcgatc ctccagggtc ccgcgttctc gggccagcgt tcgtcctcga
     3061 cggccatcac cgcatcgacg tcgatcggcg ggtagccgcg cgcttcaagc gcggccggca
     3121 cgttcaagag ccggagcatc cagtcgtagc ggaagggcgt ggcgaggaac gcctcggggg
     3181 tggcgagcgc cagcgggtcc gacgggggtc cggaccattc cacccatttg cccagcccgc
     3241 ggaacccccg gaaatactgg agcaacgcgc gttgcgctac cggtgtcgtc gtgaagaacg
     3301 cgttgcagga caatccgaac gcgacgtcga gcggaccggg gtcggcgtca cgggagaacg
     3361 cggcgaagcc ggtcacgcga tcggcttggc gcaccacgac ggcgcggtag gccgagtcgt
     3421 cgggcttggt caggatgcga acgcgccagt gttcgtcctc gaccggttcc accgggccgt
     3481 tctgattccg cacccacccg cggtaggcct cgcggatcgc ggcgaggtcg cgatcgacgt
     3541 cgaccagctc gacgctcagc gagtcgcggt ccggggcgat cgcatcgagg ggcagtcggt
     3601 gcgtggtgta cgaccccgcg atctcgaacc ccaaccgtcg gtacgggcgc agcaccgcgg
     3661 ggaagagcgc cgcgagcggc gcccctcgtt cgtgcgcccg ttggagcagc gcatcggtgc
     3721 acgcggtggc gaggccgtcc gctcgacgct cgggcaacgt cccaacgcgg gtgatcccgg
     3781 agcaggcgat gccgcggccg ccgaaccact gatcgaaccg gaactccccg gcagtggcga
     3841 ccacgcgatc gtcttcgagg gcgacgcgga tgtcgtcgag cttgaggagg ggcgcattga
     3901 gcagggcgcg ttcgagcggg aagttgatcg acgtcgacag cacacccgcg atctgttcgc
     3961 ggtgggc
//
LOCUS       DATDBZ010000352         5133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2173645, whole genome shotgun sequence.
ACCESSION   DATDBZ010000352 DATDBZ010000000
VERSION     DATDBZ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173645"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VIX62_08530"
     CDS             <1..728
                     /locus_tag="VIX62_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017612754.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEY5860315.1"
                     /translation="IATAPNVEFRVHRGDELEIVHNPAEMPAPGTYDSIEEPMVYATM
                     ITPADYLGAVMETSQSRRGEMVDMSYITPERVEIHYVLPLAEIVFDFFDQLKSRTKGY
                     ASLDYEPWGYQEANLVRVDVLLHGEPVDAFSTIVHRDKAYTYGKQMVERLKDLIPRQL
                     FDVAIQAAIGAKIIARETVKAKRKDVTAKCYGGDISRKRKLLEQQKKGKARMRRVGRV
                     DVPQEAFIAALRVEDGSKRDTKK"
     gene            725..1876
                     /gene="hemW"
                     /locus_tag="VIX62_08535"
     CDS             725..1876
                     /gene="hemW"
                     /locus_tag="VIX62_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY5860316.1"
                     /translation="MTDGDERAEEPVGIYVHVPFCLTRCGYCDFNAYAGLDHLAPRYL
                     RALIAEAALAVPAWDGAEVTSVFLGGGTPTMQTPEDLASLLVHLRETFDVAADAEITT
                     EANPDTVDEASLRALRGAGYERLSMGAQSFDVAVLASLERLHSPDSVRAAFAAARAAG
                     YDNVNLDLIYGANGETIDSWERTLQETIALAPEHISAYALTVEPATPLGRAVASGATP
                     EPDPDLQADMFGVACEQLAAAGYRHYEVSNWAKPGFECRHNLGYWERRPYLGLGAGAH
                     SYRDDHRWWNTRPPEEYMERVERGELPVGGEEHLDPSDAYLEEVFLRMRILEGIPSSW
                     IDEQVSAPFIESGLIADEFGTLVPTERGMLLLNELVLALTADGRPSHAD"
     gene            2019..3536
                     /locus_tag="VIX62_08540"
     CDS             2019..3536
                     /locus_tag="VIX62_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY5860317.1"
                     /translation="MRRLPPPVLAVLLAFAALAVPLVASVSASAQPPASVAAPAPERP
                     NVLLIVTDDQRWDTLDAMPVVQQELMSRGVTFSHSFVSNPLCCPSRASILTGNYSHTT
                     GVYRQTTPYGAFPSFHDASTLATWMHDAGYRTGLFGKYIDAYQSDALSGYVPPGWDRW
                     VAFVHSGYYTYGLTVDGEPEFHGEDPSTDYSTTVLGDAAERFILSAPEDQPVFTEFAP
                     AAPHDPATAEPTFDGSFRDIAKWRPPSYDEADVSDKPAYMQALPRFSSEWMAWVDGLR
                     RRQYETLQSVDVQIGRLLDALEETGRLENTLIIFTSDNGLLWGEHRWMKKEVPYDEAL
                     RVPMVVRYDPLAIEPRTDDHLIVNVDIAPTIASIASVPHPLVEGMSLEPLLASADAPW
                     REDFLIEHMEGSNPVPSYCGVRTETQKYVRYATGERELYDLSEDPYEMVNLVDDPARS
                     TDVATLATRLDELCDPPPPGMDEQGGTPAAVALVGVAGASGGAAYLSRRRRRSTR"
     gene            3546..4277
                     /locus_tag="VIX62_08545"
     CDS             3546..4277
                     /locus_tag="VIX62_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860318.1"
                     /translation="MPTVSCPSCATRQSVDAAATSYTCTSCGKSWDFVVCSSCGSRYH
                     AKPGATGWTCPNCGTAHGTAAAPPPPATPTPAPAMSVSGDDLDAVPPEGLAGPQQPFG
                     TPEPGSAFPMPQADRRGGFPRWGFVVIAAVVVAIVGVFLLTRGGDDAPSETPAPSADE
                     AIATMCTDVQQSTQVLRDDALGRAQDTLKQDAAAIKQAGDPATAKQVKVLIAKIEDLR
                     TALQDQAPTEDATAALLDAVSALPC"
     gene            4331..>5133
                     /gene="hrcA"
                     /locus_tag="VIX62_08550"
     CDS             4331..>5133
                     /gene="hrcA"
                     /locus_tag="VIX62_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY5860319.1"
                     /translation="MADRDRPELGPRKSAVLRAVVEQYVRSGEPVGSETIAEAAGLGV
                     SSATIRNEMAALEEQGYLRHPHTSAGRIPTDAGYRHYVDSLPAGGKLRDAQRRLIAGY
                     FTEAIVDLEEVLKGSVQLLSRLTQYAGLAVPPSAIEEPVMRLELIDLGPTVMILAVGQ
                     HGRVDKRVIDRPESIEAASLAEAEGKLATLRGQTYTAAQAHLLQLAAESRPDQHDLLL
                     HVAELLREATQGDGSTHVVVGGASNLADEAQQMRRETLRRLFETLERET"
BASE COUNT          851 a         1805 c         1725 g          752 t
ORIGIN      
        1 tgatcgcgac cgcgccgaac gtcgagttcc gcgtgcaccg cggcgacgag ctggagatcg
       61 tgcacaaccc ggcggagatg cccgcgcccg gcacgtacga ctcgatcgaa gaaccgatgg
      121 tctacgccac gatgatcacg ccggccgact atctcggcgc cgtgatggag acgagccaga
      181 gccgccgcgg cgagatggtc gacatgagct acatcacgcc cgagcgcgtc gagatccact
      241 acgtgctgcc gctggccgag atcgtcttcg acttcttcga tcagctgaag tcgcgcacga
      301 agggctacgc gagtctcgac tacgaaccgt ggggctacca ggaggcgaac ctggtccgcg
      361 tcgacgtcct gctgcacggc gaaccggtgg atgcgttcag cacgatcgtg caccgcgaca
      421 aggcctacac ctacggcaag cagatggtcg aacgactgaa ggacctgatc ccgcgccagc
      481 tgttcgacgt ggcgatccag gcggcgatcg gcgccaagat catcgcccgc gagaccgtca
      541 aggccaagcg caaggacgtc acggccaagt gttacggcgg tgacatcagc cgcaagcgca
      601 agctgctcga gcagcagaag aagggcaagg cgcgcatgcg ccgggtcggg cgtgtcgacg
      661 tgccgcagga agccttcatc gccgcattgc gggtggagga cggcagcaag cgggacacga
      721 agaagtgacc gacggcgacg agcgcgccga ggaaccggtc ggcatctacg tccacgttcc
      781 gttctgtctc acccggtgcg gctactgcga cttcaacgcc tacgccggcc tggaccacct
      841 ggcgcctcgc tacctgcggg cgctgatagc cgaggcggcg ctcgccgtcc cggcgtggga
      901 cggggcggag gtgacgagcg tgttcctcgg gggcggcacg ccgacgatgc agacgccgga
      961 ggatctcgca tcgctcctgg tccaccttcg cgagaccttc gacgtggcgg ccgacgccga
     1021 gatcaccacg gaggcgaacc ccgacaccgt ggacgaggcc tccctccgtg cgctgcgcgg
     1081 tgccgggtac gaacgcctgt cgatgggcgc ccagtcgttc gacgtggcgg tgctggcgtc
     1141 gctcgaacgg ctgcactcgc ccgacagcgt gcgcgccgca ttcgcggctg cccgcgccgc
     1201 cgggtacgac aacgtgaacc tcgacctgat ctacggcgcg aacggcgaga cgatcgattc
     1261 ctgggaacgc acgctgcagg agacgatcgc gctggccccc gagcacatca gcgcctacgc
     1321 gctcaccgtg gaaccggcca caccgctcgg ccgcgccgtg gcgagcggcg ccacgcccga
     1381 gcccgatccc gacctgcagg ccgacatgtt cggtgtggcc tgcgaacagc tcgctgccgc
     1441 cggctatcgt cactacgagg tctccaactg ggccaagccg gggttcgaat gccgtcacaa
     1501 cctcggctac tgggagcgtc ggccctacct cggtctcggc gcaggcgcgc attcctaccg
     1561 cgacgatcat cggtggtgga acacccgccc gcccgaggag tacatggagc gcgtcgaacg
     1621 cggcgaactc ccggtcgggg gagaggagca tctcgacccc tccgacgcct atctcgaaga
     1681 ggtgttcctg cgcatgcgca tcctcgaggg gatcccgtcc agctggatcg acgagcaggt
     1741 gtcggcgccg ttcatcgaga gcggtctgat cgccgatgag ttcggcacgc tggtgcccac
     1801 cgagcgcggc atgctgctcc tgaacgaact ggtcctggcc ctcacggccg acgggcgtcc
     1861 gagccacgcc gactgagccc tgttgttgac ggatcgtcac acggcttgca gggtttcgcc
     1921 ttcacgacac cctatgctgg gtgcgctccg ttgcctcccg atggacggat ccaaccgatc
     1981 accgcaacga aaggatgacg ctcgcgcgtc ttgggttcgt gaggagactt cccccgcccg
     2041 tcctggctgt gctgctggcg ttcgccgccc tcgcggttcc gctggtggcc tcggtgagcg
     2101 cgtcggccca gcccccggcc agcgttgccg cccccgcccc cgagcgtcct aacgtgctgt
     2161 tgatcgtcac cgacgaccaa cgctgggaca cgctcgacgc gatgccggtg gtgcagcagg
     2221 aactgatgag ccgcggcgtc acgttctccc attcgttcgt gtcgaacccg ctgtgttgtc
     2281 cgagccgggc gagcatcctc acgggcaact actcgcacac cacgggcgtc taccggcaga
     2341 ccacgccgta cggcgcgttc ccctcgttcc acgatgcctc gacgctggcc acgtggatgc
     2401 acgacgctgg ctatcgcacc ggcctgttcg gcaagtacat cgacgcctat cagagcgatg
     2461 cgttgagcgg gtacgtcccg cccgggtggg atcgatgggt cgccttcgtg cactccggct
     2521 actacacgta cgggctcacg gtggacggcg agcccgagtt ccacggggag gatccgtcga
     2581 ccgactactc gaccacggtg ctgggcgacg ccgcggagcg gttcatcctg tcggcgcccg
     2641 aggaccaacc ggtgttcacc gagttcgcgc cggccgcgcc gcacgacccc gcgacggccg
     2701 agcccacgtt cgacggatcg ttccgcgaca tcgcgaagtg gcgtccgcct tcctacgacg
     2761 aggccgacgt ctcggacaag cccgcgtaca tgcaggcgct cccgcgcttc tcgagcgagt
     2821 ggatggcatg ggtcgacgga ctgcgccgac gccagtacga gaccctgcaa agcgtcgacg
     2881 tacagatcgg acgtctcctc gacgcgctcg aggagaccgg ccgcctggag aacacgctga
     2941 tcatcttcac ctcggacaac ggtctgctgt ggggcgagca ccggtggatg aagaaggagg
     3001 tcccctacga cgaggcgctg cgggtgccca tggtggtgcg gtacgacccc ctggcgatcg
     3061 agccgcgcac cgacgatcac ctgatcgtca acgtcgacat cgccccgacg atcgcctcga
     3121 tcgcatccgt gccgcacccc ctcgtcgaag gcatgagcct ggagccgctc ctggcgagcg
     3181 ccgacgcccc gtggcgcgag gatttcctga tcgagcacat ggagggctcc aacccggtgc
     3241 cctcgtactg cggtgtccgc accgagacgc agaagtacgt gcgctacgcg acgggggagc
     3301 gggagctcta cgacctgtcc gaggacccct acgagatggt caacctggtt gacgatcctg
     3361 cccggtcgac cgacgtagcc accctcgcta cccgcctcga cgagctctgc gatccgcccc
     3421 caccgggcat ggacgagcag ggtgggacgc ccgcggccgt cgctctggtg ggggtcgccg
     3481 gcgccagcgg gggcgccgcc tacctgtccc ggcggcgccg ccggagcacg cgatagagtg
     3541 gcccgatgcc gaccgtcagc tgtccgtcgt gcgccacccg ccagagcgtc gacgctgcgg
     3601 ccacctccta cacgtgcacg tcatgcggca agagctggga cttcgtggtg tgttcgtcgt
     3661 gcggatcgcg ctaccacgcc aagcccggcg ccaccggatg gacatgcccg aactgtggga
     3721 ccgcgcacgg gacggcagcg gcgccgccac caccggcgac ccccaccccc gctcccgcga
     3781 tgtcggtgtc cggcgacgac ctcgatgcgg taccgcccga aggcctggcg ggtccacagc
     3841 agcccttcgg cacgcccgag cccggttccg cgttcccgat gccccaggcc gaccgacgcg
     3901 gaggcttccc ccgatggggc ttcgtggtga tcgcggcggt ggtcgtggcg atcgtcgggg
     3961 tgttcctgct cacgcgtgga ggtgacgacg cgccgagcga gacacccgcg ccgagcgctg
     4021 acgaggcgat cgccacgatg tgcaccgacg tccagcagtc cacccaggtc cttcgcgacg
     4081 acgcgctcgg ccgcgcccag gacacgctca agcaggacgc cgccgcgatc aagcaggccg
     4141 gcgacccggc aaccgcgaag caggtcaagg tcctgatcgc gaagatcgag gacctgcgaa
     4201 cggccctcca ggatcaggcg ccgaccgagg atgccaccgc agcgttgctg gacgccgtga
     4261 gcgcccttcc ctgctgagtt cctctggggg ttagcactcg gccgaggtga gtgctacgta
     4321 tcatggaccc atggcggatc gcgatcgacc ggagctgggc ccacgcaagt cggcggtgct
     4381 ccgtgctgtg gtggagcagt acgtgcgtag cggcgagccc gtgggcagcg agacgatcgc
     4441 cgaggccgcg ggcctgggcg tctcgagcgc cacgatccgt aacgagatgg ccgcgctcga
     4501 agagcagggc tacctccgtc acccgcatac gtccgcaggg cggatcccca ccgatgccgg
     4561 ctaccgccac tacgtggact cgctcccggc cgggggcaag ctccgcgacg cgcaacgccg
     4621 tctgatcgcc ggctacttca ccgaggcgat cgtggatctc gaagaggtgc tgaagggcag
     4681 cgtgcagctg ctctcacgcc tcacgcaata cgcgggattg gcggtgcccc cgtcggccat
     4741 cgaggaaccg gtgatgcgcc tggagctgat cgacctcggc cccacggtca tgatcctggc
     4801 ggtgggccag cacggtcggg tggacaagcg cgtgatcgac cggcccgaga gcatcgaagc
     4861 cgcgagcctg gcggaggccg aagggaaact ggccacgctg cgcggtcaga cctacacggc
     4921 ggcccaggcc catctcctgc aactggccgc cgagagccgg cccgatcaac acgacctgtt
     4981 gctgcatgtc gccgagctgt tgcgggaggc cacgcagggt gacggttcca cccacgtcgt
     5041 ggtgggcggc gcctccaacc tggccgacga agcccagcag atgcgacgcg agaccctccg
     5101 ccggctgttc gagacgctgg aacgcgaaac cga
//
LOCUS       DATDBZ010000353         2743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2976141, whole genome shotgun sequence.
ACCESSION   DATDBZ010000353 DATDBZ010000000
VERSION     DATDBZ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2976141"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VIX62_08555"
     CDS             <1..313
                     /locus_tag="VIX62_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY5860320.1"
                     /translation="LIRSASDPDLFADPFNGPRLLDHDGCRALFSGVAKDPAHWNEAY
                     LAPTSRRDMVVRILNNLKVIHRQRNDYLRLGRVMRMRSTVPGLGEREREEFRRLMAPM
                     N"
     gene            395..1600
                     /locus_tag="VIX62_08560"
     CDS             395..1600
                     /locus_tag="VIX62_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5860321.1"
                     /translation="MTVTESLPSAEEQRVTDLVDTLLQDVPPSSTDSRTFLGAQFDMG
                     LAWVHFPEGQGGLGLNPKLQRTINERIFAAGGPNPVGRNPIGHGMCAPTVVTWGSEAQ
                     KQRYLRPLFTGEEIWCQLFSEPGSGSDFAGLSSRGVKDGDEWIVNGQKVWTTLAHLSR
                     WGLLVVRTAPEAVKHAGLTAFVVDMHDPTVEVRPLYQITGEAEFNEVYFTDTRIPASE
                     LLGQPGDGWRVSLTTLMNERVSIGGSIPQKGSGAIRDLVTAWQKTPDDRRDAATLDEV
                     MKLWTRAEVLRLTNIRASQNRKMGDPGPEGSIGKMASADLNKDIYAKVVDVMGAEGML
                     YGSYERTRPETAMAFENPQKAFLRSRANSIEGGTTEVMKNILGERVLGLPGDVRVDRE
                     VPWSQVPRN"
     gene            complement(1633..2025)
                     /locus_tag="VIX62_08565"
     CDS             complement(1633..2025)
                     /locus_tag="VIX62_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860322.1"
                     /translation="MATLRLFLHVLAATVWVGGQLTLAGLVPGLRAIAPDAPRIVARR
                     FNRIAWPAFAALILTGMWNIAAIDVADTSTEYQVTLFLHITIAAISGISAAVHAGART
                     KLVLAGAGALSGLSALGALFLGVLLRSG"
     gene            complement(2046..>2743)
                     /locus_tag="VIX62_08570"
     CDS             complement(2046..>2743)
                     /locus_tag="VIX62_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY5860323.1"
                     /translation="GKGFRIVYVGHEGHEEAVGTMAVAPESIHRVESVAEVAALPQFD
                     QPVALLAQTTLSHRDWADVAEATKLRFPEVWTPGRSDLCFATTNRQSALMAMAPRCDA
                     LVVIGSANSSNTRALEKLAGEAGCPRVYRINHVDELPNDLFGTVGVTAGASAPEELVL
                     QVIAHLAPHHGVEEVEVTDEDEYFPPPRNIRDLQTTIETSATVMVGGSLPARPVVEDR
                     VLAASDVLAELHN"
BASE COUNT          451 a          922 c          954 g          416 t
ORIGIN      
        1 cctgatccgc tcggcctctg atccggacct gttcgccgat cccttcaacg gccctcggct
       61 gcttgatcac gacgggtgtc gggcgctgtt ctccggggtg gccaaggatc ccgcccattg
      121 gaacgaggcg tacctggccc cgacgagcag gcgcgacatg gtggtgcgca tcctgaacaa
      181 cctgaaggtc atccaccggc agcgcaacga ctacctccgg ctcggccggg tgatgcggat
      241 gcgcagcacg gtgccgggcc tcggcgagcg ggagcgggag gagttccggc ggctgatggc
      301 tcccatgaac tgagagggcc gcacggttgc ggctcgctca ggtcgtccca gtaacgtgaa
      361 cctccgtttc gagcacgtga ggatgcgcca accgatgacc gtcaccgaat cactcccgtc
      421 cgcggaggag cagcgggtca ccgatctggt cgacacgctc ctgcaggatg tcccgccgtc
      481 gtcgaccgac tcccgcacct tcctcggcgc ccagttcgac atgggcctgg cctgggtcca
      541 cttccccgag ggccagggcg gactcgggct caatcccaag ctgcagcgca cgatcaacga
      601 gcggatcttc gccgccggcg gcccgaaccc ggtgggccgc aacccgatcg gccacgggat
      661 gtgcgcaccg acggtcgtca cgtgggggag cgaggcccag aagcagcgtt acctccgtcc
      721 gctgttcacc ggcgaggaga tctggtgcca gctcttctcg gagccaggtt cgggttccga
      781 cttcgccggc ctgtcctccc gcggtgtcaa ggacggcgac gagtggatcg tcaacgggca
      841 gaaggtgtgg acgactctcg cccatctctc ccgctgggga ctcctcgtcg tccgcactgc
      901 gccggaagcg gtgaagcacg ccgggctgac ggcgttcgtc gtcgacatgc acgatccgac
      961 cgtcgaggtc cgcccgctct accagatcac cggcgaggcc gagttcaacg aggtgtactt
     1021 caccgacacc cgcatcccgg cgtcggaact gctcggccag cccggcgacg gctggcgggt
     1081 ctcgctcacg acgttgatga acgagcgcgt ttcgatcggc ggctcgatcc cgcagaaggg
     1141 ctcgggcgcg atccgagacc tcgtgacggc gtggcagaag acgcctgacg atcggcgcga
     1201 tgccgccacg ctcgacgagg tgatgaagct ctggacccgg gccgaggtgc tccggctcac
     1261 gaacatccgc gcctcgcaga accgcaagat gggcgacccg ggccccgagg gctccatcgg
     1321 caagatggcc tccgccgatc tcaacaagga catctacgcc aaggtcgtcg acgtgatggg
     1381 ggccgagggg atgctctacg ggtcgtacga gcgaacccgg cccgagacgg caatggcgtt
     1441 cgagaacccg cagaaggcgt tcctgcgcag tcgggccaac tcgatcgagg ggggcaccac
     1501 cgaggtgatg aagaacatcc tcggcgagcg cgtgctcggc ctcccgggcg atgtccgtgt
     1561 cgaccgcgag gtcccgtgga gccaggtgcc acgcaactga gaccgcctcg taactgagac
     1621 cggcgacgct cctcagccgg agcgcaggag caccccgagg aacaacgcgc cgagcgcgct
     1681 gagaccgctc aacgcacccg ctccagccag gacgagcttt gttctcgccc cggcgtgcac
     1741 ggcggcgctg atgcccgaga tggcagcgat cgtgatgtgc aggaacaggg tcacctggta
     1801 ctcggtgctc gtgtcggcga catcgatggc ggcgatgttc cacatcccgg tcaggatcag
     1861 cgccgcgaat gccggccagg cgatgcggtt gaaccggcgg gccacgatgc gcggtgcgtc
     1921 gggcgcgatc gctcggagcc cgggcacgag gccggcgagc gtcagctgac cgccgaccca
     1981 gacggtggcc gcgagcacgt ggaggaacag gcggagcgtc gccaccgacg gggagagcat
     2041 cgcgatcagt tgtgcagttc ggcgagcacg tcgctggcgg ccaggacccg gtcctcgacc
     2101 acgggccgag cgggcaagct gccgccgacc atcacggtgg ccgaggtctc gatggtggtc
     2161 tgcaggtctc ggatgttgcg cggcggcggg aagtactcgt cttcatccgt gacttcgacc
     2221 tcctcgacgc cgtggtgcgg cgcgaggtgg gcgatgactt gcaggacgag ctcctcgggc
     2281 gccgatgcac cggcggtcac gccgacggtg ccgaacagat cgttgggtag ctcgtcgacg
     2341 tggttgatgc ggtacacccg ggggcatccg gcctcgccgg ccagcttttc gagcgccctc
     2401 gtgttggacg agttggccga cccgatgacg acgagcgcat cgcatcgggg cgccatcgcc
     2461 atcaacgccg actgccggtt cgtcgtggcg aagcagaggt cgcttcgccc gggtgtccag
     2521 acctctggga agcggagctt cgtcgcctcg gccacatcgg cccagtcgcg atgggagagc
     2581 gtggtctgcg ccaagagagc aaccggttga tcgaactgcg gcagcgccgc gacttcggcg
     2641 accgattcga cgcgatggat cgactcgggc gcgaccgcca tcgtgccgac ggcctcctcg
     2701 tgaccctcgt ggccgacgta gacgatgcgg aagcccttgc ccg
//
LOCUS       DATDBZ010000354         2608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1962796, whole genome shotgun sequence.
ACCESSION   DATDBZ010000354 DATDBZ010000000
VERSION     DATDBZ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962796"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..431)
                     /locus_tag="VIX62_08575"
     CDS             complement(12..431)
                     /locus_tag="VIX62_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY5860324.1"
                     /translation="MHEFPTDAWFQVLIERINASEAYREAAAGWEGDVAFLVEAEPDR
                     RVPEDVWGLLDLWHGACRGGGMIDAERGRNAAFVISAPYARWKDVVLGELDPVRAMMQ
                     GKLRVRGDLPMIVRYVKAADELVHLCSTVPTRFPDED"
     gene            complement(432..1217)
                     /locus_tag="VIX62_08580"
     CDS             complement(432..1217)
                     /locus_tag="VIX62_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY5860325.1"
                     /translation="MLGESPDTDRGRGMHGGPPVGWSFWGGLGIFLLGNVLIGQVLVA
                     GAILMAIGFDQVSTTGAAGMPELAATLGADIATVATIVVWFSARHQGWVQALRLPEKG
                     KRLKEIAIAIGLGPLVYAGIAFLVVPIIAIFLESIAGQGASTPEQIDAEGLSSAGRIL
                     TVLVAVVVAPITEELVFRGVLFRSIRDRSGFWWGAVVSSLLFGLVHFVPAPWQDTLLL
                     QATMVFTGLALAAIYEWRGNLLSCIVTHMSFNVVGVILILVVG"
     gene            complement(1279..2550)
                     /gene="murA"
                     /locus_tag="VIX62_08585"
     CDS             complement(1279..2550)
                     /gene="murA"
                     /locus_tag="VIX62_08585"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY5860326.1"
                     /translation="MERLLVTGGARLAGSIRISGAKNSALKLMAAALLAPGRTVLHNV
                     PRIQDCFTMGEVLEHLGAGVAWDGDTVTIDATTLTSVEAPYELVRRMRASTAVLGSLL
                     ARAGEARVAMPGGDEIGSRPIDLHIAGLRRMGAVIGSEHGFLVATTEGLRGAAITLDY
                     PSVGATENLMMAAVRARGTTVIDNAAREPEIADIAEFLVSMGARIDGAGGSTIEIDGV
                     DTLVPAEHMTIADRIETGTWAAAAVATGGDLTLAGARADHLDLFLHKLSDAGAIVEHT
                     PGGLRIRQATRPRAIDFVTLPYPGVATDFQPILMAMLATADGTSIATENVFEGRFLYV
                     DELRRMGADIRTEGHHAVIRGMPRLSAAPVRALDIRAGAAMVIAALVADGTTEVADMH
                     HVDRGYEDFEDKLIGVGAEVRRIGEPASAPG"
BASE COUNT          454 a          893 c          866 g          395 t
ORIGIN      
        1 tcgcatccgg gtcagtcctc gtcggggaac cgggtcggca ccgtgctgca caggtgcacc
       61 agttcgtcgg ccgccttgac gtagcgcacg atcatgggca gatcgccccg caccctcagc
      121 ttgccttgca tcatggcgcg cactgggtcg agttcaccca ggacgacgtc cttccaccgg
      181 gcgtagggag cgctgatcac gaatgcggcg ttgcgtccgc gctccgcgtc gatcatgccc
      241 ccgccacggc acgcgccgtg ccataggtcg aggagacccc agacgtcctc gggcacgcgc
      301 cggtcgggtt cggcttcgac caagaatgcg acgtcgccct cccaccctgc cgcggcttcg
      361 cggtaggcct cagaggcgtt gatccgctcg atcagcacct ggaaccaggc gtcggtgggg
      421 aactcatgca cctagcccac caccaggatc aagatgaccc cgacgacgtt gaacgacatg
      481 tgggtcacga tgcacgacag caggttgccc cgccattcgt agatcgcggc gagcgccaac
      541 cccgtgaaga ccatggtggc ctgcagcagc aacgtgtcct gccacggtgc cgggacgaag
      601 tggaccagcc cgaacaacaa cgaagagacg accgcccccc accagaaacc gctgcgatcg
      661 cggatcgaac ggaagaggac gccgcggaac accagttcct cggtgatcgg tgccaccacc
      721 acggcgacga gcacggtgag gatgcgtccg gccgaggaca agccttcggc gtcgatctgc
      781 tccggcgtgc tggcaccctg gcccgcgatc gactccagga agatggcgat gatcggcacc
      841 accaagaatg cgatccccgc gtagacgagc ggccccaggc cgatcgcgat cgcgatctct
      901 ttcagccgtt tccccttctc ggggagccgg agtgcctgta cccacccctg gtgacgggcc
      961 gaaaaccaca ccacgatcgt cgccacggtc gcgatgtcgg cgccgagcgt ggctgccagt
     1021 tccggcatcc cggcagcccc ggtggtcgac acctgatcga agccgatcgc catgaggatg
     1081 gcgccggcga cgaggacctg accgatcagc acgttgccca gcaggaagat gccgagcccg
     1141 ccccagaatg accaccccac cggtggacct ccgtgcatgc cgcgcccacg atcggtgtcg
     1201 gggctctcac cgagcatcgg tggagcggac cagcccagat cgggacgagg cggtggttcg
     1261 agcatcatcg ggtgacggtc atcccggcgc cgaagcgggc tcaccgatac ggcggacttc
     1321 ggcacccacg ccgatgagct tgtcctcgaa gtcttcgtag ccacgatcga cgtggtgcat
     1381 atcggcgacc tcggtggtgc cgtctgcgac caacgcggcg atcaccatgg cggcgccggc
     1441 ccggatatcg agcgcgcgta cgggggctgc ggacagtctc ggcatgcccc ggatcaccgc
     1501 gtggtgtcct tcggttctga tgtcggcgcc catgcgacgg agctcgtcca cgtacaggaa
     1561 ccgtccctcg aagacgttct cggtcgcgat gctggtgccg tcggcggtcg cgagcatggc
     1621 catgaggatc ggttggaagt ctgttgccac ccccgggtag ggcagcgtga cgaagtcgat
     1681 cgcgcgaggg cgcgtggctt ggcggatccg gagcccaccc ggggtgtgtt cgacgatggc
     1741 accggcatcg ctgagcttgt gcaggaacag gtcgaggtga tcggcgcggg ctcccgcaag
     1801 ggtgaggtcg ccgccggtgg cgaccgccgc ggcggcccac gtgccggttt cgatgcgatc
     1861 ggcgatcgtc atgtgctcag cgggcaccaa ggtgtccacg ccatcgatct cgatcgtgct
     1921 cccgccggct ccgtcgatcc gcgcccccat ggacacgagg aactcggcga tatcggcgat
     1981 ctcaggctcg cgggcagcgt tgtcgatgac ggtcgtgcca cgcgcccgaa cggcagccat
     2041 catgaggttc tcggtggccc cgacgctcgg gtagtccaac gtgatcgccg caccgcgcag
     2101 cccttccgtc gtcgcgacca ggaagccgtg ctcagaaccg atcacggcgc ccatgcggcg
     2161 gagaccggcg atgtgcagat cgatgggacg cgaaccgatc tcgtccccgc cgggcatcgc
     2221 aaccctcgcc tctcccgcac gagccaagag agagccgagg accgcggtcg aggcacgcat
     2281 gcgacgcacc aactcgtacg gcgcctcgac cgacgtcaac gtggtggcat cgatcgtgac
     2341 cgtgtcgcca tcccacgcaa cgccggcgcc cagatgctcc aggacctcac ccatcgtgaa
     2401 gcagtcctgg atgcggggaa cgttgtgcaa cacggttcgg ccgggcgcga gcaaggcggc
     2461 agccatgagc ttgagtgcgg agttcttggc gccggagatc ctgatcgaac ccgccaggcg
     2521 cgctcctcca gtcaccagga gacgttccat aggggaatca gtgtaggggg cgggcctgcc
     2581 tggcggtgcg aagcggcggt gctacccc
//
LOCUS       DATDBZ010000355         2452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_520036, whole genome shotgun sequence.
ACCESSION   DATDBZ010000355 DATDBZ010000000
VERSION     DATDBZ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520036"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VIX62_08590"
     CDS             complement(<1..1207)
                     /locus_tag="VIX62_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEY5860327.1"
                     /translation="MLANLTSTTSLFASEGGSTGNFADLHVEPWVWAAFLTFVAVLLV
                     ADLLLVHKTAHVISTKEAAIESAIWITIGVSFTFVVWGAWGGQAAGEYISGYLIEKSL
                     SVDNVFVWAIIMSYFAVPRAYQFRVLFWGVFGALVLRFIFIFAGVGLLNRFEWMLFVF
                     GGFLIFTAIRLLRHDDEEIHPENNPVLKAVRRLVPSTSEYHGQSMFTKINGKRLATPL
                     FAVLVVIETSDVVFAVDSIPAILAVSRDTFIIFSSNAFAILGLRALYFLLADLLLVHR
                     TAHVISTKEAAIESAIWISIGVAFTFVVWGAWGGQAAGEYISGYLIEKSLSVDNVFVW
                     ALIMSYFAVPRQFQFRVLFWGVFGALVLRFVFIFAGAALLQQFSWMLFVFGGFLIFTA
                     IRLLRHDDEE"
     gene            complement(1225..>2452)
                     /locus_tag="VIX62_08595"
     CDS             complement(1225..>2452)
                     /locus_tag="VIX62_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY5860328.1"
                     /translation="SVKIADHHFTAGSATVNEGDVISLDGSTGEVVLGAMALADAEPP
                     PEFRTILAWADRVRKGKLAVRANADTGADAAVALGFGAEGIGLCRTEHMFLAEDRLPI
                     VRAMILAHTPEEESAALEHLRVAQKADFLEILAAMDGLPVTVRLLDPPLHEFLPSVEE
                     LLVQKATSGLNAEETALLAAAESWREQNPMLGTRGVRLGVLKPGLYEMQVRALMEAAV
                     ELHAVGRHPVVEIMIPLTVTREEMLLARGWVEEAIAAVPGTDAKGLAVTIGTMIETPR
                     AALCADEIAEVADFFSFGTNDLTQMAFGFSRDDVESRMMPAYLEQGLLRRNPFETIDR
                     DGVGELVALAVERGRTAKPKLKLGVCGEHGGDPESIELFHRVGLDYVSCSPYRVPIAR
                     LAAAQAILDATTTDTR"
BASE COUNT          439 a          834 c          825 g          354 t
ORIGIN      
        1 tctcctcgtc gtcgtggcgg aggagccgga tggcggtgaa gatcaggaac ccgccgaaca
       61 cgaagagcat ccacgagaac tgctggagca gcgcggcgcc ggcgaagatg aacacgaacc
      121 ggagcaccag cgccccgaac acgccccaga acagcacccg gaactggaac tgccgcggca
      181 ccgcgaagta gctcatgatg agcgcccaca cgaagacgtt gtcgacggac aagctcttct
      241 cgatcaggta gccggagatg tactcgccgg cggcctggcc tccccacgct ccccacacga
      301 cgaacgtgaa ggcgacaccg atcgagatcc agatggcgct ctcgatcgcc gcttccttgg
      361 tgctgatgac gtgcgcggtc cggtgcacga gcagcaggtc ggcgagcagg aagtagagcg
      421 ctcgcaggcc gaggatcgcg aaggcgttcg agctgaagat gatgaaggtg tcgcggctga
      481 cggcgaggat cgccgggatc gagtccaccg caaagaccac gtcgctcgtc tcgatcacga
      541 cgagcaccgc gaacagcggg gtggccagcc gcttgccgtt gatcttcgtg aacatcgact
      601 ggccgtggta ctcgctcgtg gacggcacga ggcgccgcac ggccttcagc accggattgt
      661 tctcggggtg gatctcctcg tcgtcgtggc gaagcagccg gatcgccgtg aagatcagga
      721 acccgccgaa cacgaagagc atccactcga accggttgag caacccgacg ccggcgaaga
      781 tgaagatgaa gcggagcacc agggcgccga acacgcccca gaacagcacc cggaactggt
      841 acgccctcgg gacggcgaag tagctcatga tgatcgccca cacgaagacg ttgtcgacgg
      901 acaggctctt ctcgatcaga tagccggaga tgtactcccc ggccgcttgg ccgccccacg
      961 cgccccagac gacgaacgtg aacgagacgc cgatcgtgat ccagatcgcg ctctcgatgg
     1021 ccgcttcctt ggtgctgatg acgtgggccg tcttgtgcac cagcaggagg tcggccacca
     1081 ggagcacggc cacaaacgtc aggaaggcag cccacaccca cggctccacg tggaggtcgg
     1141 cgaagttccc ggtggatccc ccttccgagg cgaacagcga ggtcgtcgag gtcaggttgg
     1201 cgagcacggt gatccttgga gctcctagcg ggtgtccgtc gtggtcgcgt ccaggatggc
     1261 ctgtgccgcc gccaatcggg caatcggcac gcgatacggc gagcaggaca cgtagtcgag
     1321 gcccacgcgg tggaacagct cgatcgactc cggatcgcca ccgtgctcac cgcacacacc
     1381 cagcttgagc ttcggcttgg cggtgcggcc ccgctcgacg gcgagcgcca ccagctcgcc
     1441 cacgccgtct cggtcaatgg tctcgaacgg gttgcgccgg agcaggccct gctcgaggta
     1501 ggcgggcatc atgcggctct cgacgtcgtc ccggctgaac ccgaacgcca tctgcgtgag
     1561 gtcgttggtg ccgaaggaga agaagtcggc cacctcggcg atctcgtctg cgcacaacgc
     1621 agcccgcggc gtctcgatca tcgtgccgat cgtgacggcc agccccttgg cgtcggtgcc
     1681 gggcaccgcc gcgatcgcct cctcgaccca cccacgggcg aggagcatct cctcccgcgt
     1741 gacggtgagc gggatcatga tctccacgac cgggtgccgg ccgacggcgt gcagctcgac
     1801 cgcggcctcc atgagcgccc gaacctgcat ctcgtagagc cccggcttga gcaccccgag
     1861 gcggacgccg cgcgtgccca gcatggggtt ctgctcacgc caggactccg ccgccgccag
     1921 cagggcggtc tcctccgcgt tcagccccga ggtggctttc tgcaccaaca gctcctccac
     1981 cgagggcagg aactcgtgca gcggcggatc gaggagccgc accgtgacgg ggaggccgtc
     2041 catcgccgcg aggatctcga ggaagtcggc cttctgcgcc acccgcaggt gctcgagggc
     2101 cgcgctctcc tcctcgggcg tgtgggcgag gatcatggct cgcacgatcg ggagccggtc
     2161 ctcggcgagg aacatgtgct ccgtgcgaca gaggccgatc ccctccgcgc cgaagccgag
     2221 ggcgacggcg gcgtccgcgc cggtgtcggc gttggcgcgc accgcgagct tccccttccg
     2281 gactcgatcc gcccaggcga ggatcgtccg gaactcggga gggggctcgg cgtccgccag
     2341 cgccatggca ccgagcacca cctcgccggt gctgccgtcg agggagatca cgtcgccctc
     2401 gttgaccgtg gccgacccgg cggtgaagtg gtggtcggcg atcttgacgg ag
//
LOCUS       DATDBZ010000356        10387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2174124, whole genome shotgun sequence.
ACCESSION   DATDBZ010000356 DATDBZ010000000
VERSION     DATDBZ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10387
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174124"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VIX62_08600"
     CDS             <1..424
                     /locus_tag="VIX62_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860329.1"
                     /translation="LRLSPTHSNVGVVGPYKDVGNNLFCKVEKTPVHPNGFLAIGRRL
                     HGTKPAIRVKEDNLGLTAARRYTLKLVRDRSTVTCTIARQGTVYGSLDYTLTKPERRA
                     FGSGKKTGIRMRLVARGTRRDEDDGRSVFDTFRVRTLS"
     gene            complement(426..905)
                     /locus_tag="VIX62_08605"
     CDS             complement(426..905)
                     /locus_tag="VIX62_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY5860330.1"
                     /translation="MWPPCRGDRVHRPRSRTVPSSPRPAEGSLTTVRALTPDDLFAIG
                     EAELGPDVEVDQDLLKRVAHEPLRTEDERDAYPGIHLKAAALLLSMLRERPFGEGNGR
                     IALLATTVFLNLNGQDVEASDADLVALVAVTHDGDLTLLQVAAALERLCVRLKLPEA"
     gene            1211..1810
                     /locus_tag="VIX62_08610"
     CDS             1211..1810
                     /locus_tag="VIX62_08610"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5860331.1"
                     /translation="MLELAILGLLKEQPMHGYQLSRELGESLGGFWRVSYGTLYPTLR
                     RLERAGEVETDAEASAGPGRRKKVYRITEQGETAFFELLQETPSDNSTEDTRFRVRLA
                     FFKYLPPETRIKLLERRRAYLEDRLSTIKGSLSTTRARVDDYTHALIEHGQTSTESDI
                     AWVDDLIAAERTRSTKTNGTERGGKERRTSTLRRKEHTS"
     gene            1807..2934
                     /locus_tag="VIX62_08615"
     CDS             1807..2934
                     /locus_tag="VIX62_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY5860332.1"
                     /translation="MSKVRIAIVGVGNCASSLIQGLEYYKDADPQDRVPGLMHVELGG
                     YHVHDVEVVAAFDVDAKKVGTDVAEAILSEPNNTIRFADVAHTGVKVNRGRTLDGLGT
                     YYRDMIAESELPEADVVQVLKDAKADVLVCYLPVGSEQAAKFYAQCAIDAGVGFVNCL
                     PVFIASTPEWAKKFADAGVPIIGDDIKSQVGATITHRVLTKLFEDRGVKLNRTYQLNF
                     GGNMDFMNMLERERLTSKKISKTQSVQSQLEKPLDKQQIHIGPSDHVPWLEDRKWAMI
                     RLEGESFGGVPLSIEMKLEVWDSPNSAGIVIDAVRCAKLAMDRGVSGPLIGPSAYFMK
                     SPPVQFSDEGAHDMVEEFILGTGEAADADWTAFFDNQTTGA"
     gene            complement(2969..3052)
                     /locus_tag="VIX62_08620"
                     /pseudo
     CDS             complement(2969..3052)
                     /locus_tag="VIX62_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229383.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(3064..3840)
                     /locus_tag="VIX62_08625"
     CDS             complement(3064..3840)
                     /locus_tag="VIX62_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY5860333.1"
                     /translation="MARFKVGVQIRPQHTTMDVLRQAWREADAMGVDTIFTWDHFYPL
                     YGPEDGAHFEGWSMLAAMACDTQQAHFGMMVTCNSYRNPDLLADMARTVDHLSHGRLI
                     LGIGSGWFERDYDAYGYEFGTAIDRLHALGDALPRIEARLQVLNPPPVQSPLPILIGG
                     AGEKVTLRIVAEHANLWNTFGPPENFAKKNAILTDWCGKVGRVPADIERTVMIRGADD
                     VDQVEDYLEAGAQHVILGAATEGEEPIDLKPLQRLLDARS"
     gene            complement(3937..4563)
                     /locus_tag="VIX62_08630"
     CDS             complement(3937..4563)
                     /locus_tag="VIX62_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY5860334.1"
                     /translation="MLLVPAALLVFTLGVSGGLPAGAGQPGTSPGTPPPDGVHVEVSD
                     FLFDPATVPLGRGQTVVFDFVGPSHHTATDSSGMELYDSGSVGAGEPSTWYTFPAAGV
                     YRFTCTPHVEMGGRVAIPMRVSPASGGPHRTFHLTWASIAAPEGFVYDVQIQKPGKDW
                     ADWRSGVVGRKTSFTPNAGKGKYRFHARMRSLDGGEAFWSAEAVIKVG"
     gene            4634..6070
                     /locus_tag="VIX62_08635"
     CDS             4634..6070
                     /locus_tag="VIX62_08635"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5860335.1"
                     /translation="MSATGERIGTTRDALRHADFRRLFSIRLISQSADGLVQAALVAS
                     LAFSPERATTASGFAIASAIVIIPYSLLGPFAGVFIDRWSRRKILVIAPLVRAAPAFL
                     ILASPTRYAFWYYAGALFVTSVNRFFLATAQAVVPRMVPTKDLLAANGIATVGGTVAL
                     LVGVFAGGLISDAFGNGAIVTAAAIMWLVTSLIAWRIRSDLRPHLLPESSDLLRHQLR
                     RVGFEFVDGITHIVRTPRAIAPITSIAVDQMGQGLVLVLALVVFRERFGTGVGSFSWL
                     IGAGGVGVFLGLATVGLLDRRFSRERIVAFSFALGGVALLLVALFLNRWSALLASFVV
                     GLTFAWKKVPIDTMVQEAVPDGLRGRVFAVYDVAYNLARLTAALLAVVLLPAIGENGA
                     AAVVGIVFLLWVPVLPRWLRNVPEIVVRVNADGEPEQVQWGGVSEPVTVLDAQGSGRW
                     RVRLADGTILAIGTLADGRWEIAEEIEP"
     gene            complement(6067..6861)
                     /locus_tag="VIX62_08640"
     CDS             complement(6067..6861)
                     /locus_tag="VIX62_08640"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEY5860336.1"
                     /translation="MSDQRAKGEAFRALHAGEPFVIPNPWDAGSARVMQALGCKALAT
                     TSSGFAFTLRRRDGEVTPDEAMAHTASVTAATEVPVSVDLENGYGPEPEQAALAITRV
                     AAAGAVGGSIEDFDRDGGRLYEVGHAADRIAAAAEAARALDVPFMLTGRAENHIRDNP
                     DLGDTIARLQAYEAAGADVLYAPGLATVDEIRAVCSAVSRPVNVLGFPGFTIGEIFEA
                     GAQRVSVGGGLTWVAVAAMADAVTALLETGDFSSLEVDVPLDRWFA"
     gene            complement(6854..7807)
                     /locus_tag="VIX62_08645"
     CDS             complement(6854..7807)
                     /locus_tag="VIX62_08645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEY5860337.1"
                     /translation="MTDHEASDTAIDPTMRAIRLSSPGGPGALELEEISVPDIAPGEA
                     LIRVHAAALTKDELEWPEDRLPAIPSYEVSGTVAAVAQDVETVSVGDSVYALTDFGRD
                     GAAADYVAVAAELLAPKPRTLDDVQSAAIPLPGLSAWQGLFDHGGLEAGQRVLITGAA
                     GGVGHLATQLARHRGAHVIGTASPASQEAARGFGAHEVIDGSDLRPLEPVDLVFDTGG
                     GATLEASPAVIRPGGRLVSVAEETPEIPADAKIETSYFVVEPNREQLVELTRLAETGD
                     LVPAIDSVFPLADARSAFERAQASGKRGKVVLRVSEDGDDV"
     gene            7877..8392
                     /locus_tag="VIX62_08650"
     CDS             7877..8392
                     /locus_tag="VIX62_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5860338.1"
                     /translation="MQHSSPDHVDEILAQWQRERPDLDASPIGVFGRLSRADRMVDTA
                     LAAGIAAHGLQPGWFDLLAALRRQGAPYELTPTRLTEATLLSSGGTTKRLDRLAEAGL
                     IERLPDPDDRRGTLVRLTRQGKRVIDRALQTHLENERRLLQVFSRDQRRTFEELLRTL
                     LDGLDAADPPA"
     gene            8579..8764
                     /locus_tag="VIX62_08655"
     CDS             8579..8764
                     /locus_tag="VIX62_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860339.1"
                     /translation="MSKHGEHQPGTVGTCLKCTRIAYEPSWYAVMLAFEERNLDTVVQ
                     LTDHAEAVTTRSGHIWT"
     gene            complement(8880..10172)
                     /locus_tag="VIX62_08660"
     CDS             complement(8880..10172)
                     /locus_tag="VIX62_08660"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5860340.1"
                     /translation="MSSKITEALGRTLSSVKSSRNFRLYLFGQTVSAIGTWMNFTASA
                     WLVLQLTHSGTALGTNIALYFLPVLLLGAYGGVLADRFDKRKILLVTQAAYAVVAFTL
                     WSLVATDVAQLWMVYVLSLASGLVTAFDNPSRQSFYVEMVGEEQVRNAVSLNSAAFTG
                     SRVIGPAIAGVLIATVGIATCFLVDAFSYIAVLAALLMMRPSELHRQEKSTRDRGHLV
                     AGLKYVWRTDDLRRPLLAMAVVFTFAFNFAVFVPLLAERTFHGDAGTFGMLSALAGVG
                     SFLGAITMASRIAAPKLRDLAWWAVACGVSLALAGAAPVLWLAALAMIPMGFTIMAFM
                     ITGNTMLQLTSVPQARGRVMALYGIVFLGSTPIGAPIAGVVGEHLGAQTGLWLSGLVA
                     AGLGVALLGLGRRHARVGRLGEDEEAVDAGAASAIASR"
     gene            complement(10169..>10387)
                     /locus_tag="VIX62_08665"
     CDS             complement(10169..>10387)
                     /locus_tag="VIX62_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5860341.1"
                     /translation="LVERGLVERTPDPLDGRVAWIQVTPAGRKLLSRVRRRHAEYLAR
                     KIKRLPPDELQTLERAALILDRITERPV"
BASE COUNT         1718 a         3609 c         3456 g         1604 t
ORIGIN      
        1 cctgcgcctg agtcccaccc actccaacgt gggggtcgtt ggtccgtaca aggacgtggg
       61 caacaacctc ttctgcaagg tggagaagac gccggtgcac cccaacgggt tcctcgcgat
      121 cgggcgacgt ctgcacggga cgaagcctgc gatccgcgtc aaggaggaca acctgggtct
      181 gaccgcggcg cggcggtaca cgctgaaact ggttcgcgac cgctcgaccg tcacctgcac
      241 catcgcgcgc caaggcacgg tctacggctc gctggactac acgctgacga agccggaacg
      301 ccgcgcgttc ggcagtggca agaagacggg catccgcatg cgactggtcg ctcgcggcac
      361 ccgacgcgac gaggacgacg ggcgttcggt tttcgacacc ttccgcgtca ggaccctgtc
      421 ctagctcaag cctcggggag cttcaggcga acgcacaacc gctcgagcgc ggcggcgacc
      481 tgcagcaggg tgagatcgcc gtcgtgcgtc accgcgacca acgcgacgag atccgcgtcg
      541 ctagcttcca cgtcctggcc gttcaggttc aggaagacgg tggtggcgag cagtgcgatg
      601 cggccgttcc cttcgccgaa cggtcgttcc cggagcatcg acaacaacag ggcagcggcc
      661 ttcaggtgga tgcccggata ggcgtcgcgt tcgtcctcgg tccggagggg ctcgtgggcg
      721 acgcgcttca gcaggtcctg gtcgacctcg acgtcgggac ccagctcggc ttcaccgatc
      781 gcgaacaggt cgtcgggcgt gagcgcgcgg accgtggtta gagatccttc agctggtcga
      841 gggctggacg gaaccgttct cgagcgcggc cggtgaaccc ggtcaccgcg gcacggtggc
      901 cacactcatc caggtagtca cgcagagcgt ggaggacgtg ctcgtcgacg ggttcacccg
      961 tggcgaacgc caacgtgccc atggcctcgc ctacttcgcg tgggagccgg atcgtgagct
     1021 gctggatggt tcacctcctg cccacagtct ggacgatgag cggggcatcg acgaccacct
     1081 ggcccgccaa ccccgagttg acgttccccg gagttccggg tagatatatc gaagcgatat
     1141 atgcatccga tatatcgcgg cgtcacgtcc gtgacgaccg aacctacccg aagggacacc
     1201 cacccacacc atgctggagc tcgcgatcct cggattgctc aaggagcagc ccatgcacgg
     1261 ctaccagctc tcccgggagc tgggtgagtc gctgggcggg ttctggcgcg tgtcctacgg
     1321 caccctctac cccaccctgc ggcgcctcga gcgcgcgggt gaggtcgaga ccgatgccga
     1381 ggcgagcgcc ggacccgggc gtcgcaagaa ggtctaccgc atcaccgaac agggcgagac
     1441 cgccttcttc gagctgctgc aggagacgcc ctccgacaac tcgaccgaag acacccgctt
     1501 ccgggtgcgc ctggccttct tcaagtacct gccgccggag acccggatca agctgctgga
     1561 acgccgccgc gcctacctgg aggaccggct gtccaccatc aagggctcgc tgagcaccac
     1621 gcgtgcgcgg gtcgacgact acacccatgc cctgatcgaa cacgggcaga cctcgaccga
     1681 gagcgacatc gcctgggtcg acgacctgat cgcggccgag cggacgcgat cgacgaagac
     1741 gaacggcacc gaacgagggg gcaaggagcg tcgcacctcg acgctccgcc ggaaggagca
     1801 cacatcatga gcaaggttcg catcgcgatc gtcggcgtgg gcaactgcgc gtcgtcactc
     1861 atccaaggcc tcgagtacta caaggacgcc gacccgcagg accgcgtccc gggcctgatg
     1921 cacgtcgagc tgggcggcta ccacgtccac gacgtcgagg tcgtggccgc gttcgacgtg
     1981 gacgccaaga aggtgggcac cgacgtcgcc gaggccatcc tgtccgagcc gaacaacacg
     2041 atccggttcg ccgatgtggc ccacacgggc gtgaaggtca atcgtggtcg cacgctcgac
     2101 ggcctgggca cctactaccg cgacatgatc gccgagtccg agctgcccga ggccgacgtg
     2161 gtccaggtgc tgaaggatgc gaaggccgac gtgctcgtgt gctacctgcc ggtcggatcc
     2221 gagcaggccg cgaagttcta cgcgcagtgc gcgatcgacg ccggcgtggg attcgtgaac
     2281 tgcttgccgg tgttcatcgc ctccacgcct gagtgggcca agaagttcgc cgacgcgggc
     2341 gtgccgatca tcggcgacga catcaagtcg caggtgggcg ccacgatcac gcaccgggtg
     2401 ctgacgaagc tgttcgagga ccgcggcgtg aagctcaacc ggacctacca gctgaacttc
     2461 ggcggcaaca tggacttcat gaacatgctc gagcgcgaac gcctcacgtc caagaagatc
     2521 tccaagacgc agtccgtgca gtcgcagctg gagaagccgc tcgacaagca gcagatccac
     2581 atcggaccgt cggaccacgt gccgtggctc gaggaccgca agtgggccat gatccggctc
     2641 gagggcgaga gcttcggcgg cgtgccgctc agcatcgaga tgaagctcga ggtgtgggac
     2701 tcacccaact ccgcgggcat cgtgatcgac gcggtgcggt gcgcgaagct ggcgatggac
     2761 cgcggcgtgt ccggcccgtt gatcggaccc agcgcctact tcatgaagtc gccgcccgtg
     2821 cagttcagtg acgagggcgc acacgacatg gtggaagagt tcatcctcgg caccggcgaa
     2881 gccgccgacg ccgactggac cgcgttcttc gacaaccaga ccacgggcgc ttgagcgcag
     2941 cgggagccct cgctgccgtg ggctccggct atcccccgcc ggtgtagagg cagaacggat
     3001 ggccggccgg gtcgaacatg acccggacgt cgtcctgagg ctggaagtcg gcctcttgcg
     3061 cgatcagctt cgagcgtcca gcagccgctg taacggtttg aggtcgatcg gctcttcgcc
     3121 ttcggtggcc gcgcccagga tgacgtgctg cgcgcccgct tcgaggtagt cctcgacttg
     3181 gtcgacgtcg tccgcgccac ggatcatcac ggtgcgctcg atatccgcgg gaacgcgccc
     3241 gaccttgccg caccaatcgg tgaggatcgc gttcttcttc gcgaagttct ccggcggacc
     3301 gaaggtgttc cagaggttcg cgtgctcggc cacgatgcgc agcgtgacct tctccccggc
     3361 gccgccgatc aggattggca acggcgactg caccggcggt gggttgagga cctgcagtcg
     3421 agcctcgatc cgcggcaggg cgtcgcccag ggcatgcagc cgatcgatcg ccgtgccgaa
     3481 ctcatatccg taggcgtcgt aatcgcgttc gaaccagccc gagccgatcc ccaggatcag
     3541 ccggccgtgc gacaggtgat cgaccgtgcg cgccatgtcg gccagcaggt ccgggttccg
     3601 gtaggagttg caggtcacca tcatgccgaa gtgcgcctgc tgggtgtcgc acgccatcgc
     3661 ggcaagcatc gaccatccct cgaagtgcgc accgtcctcc gggccgtaga gcgggtagaa
     3721 gtggtcccac gtgaagatcg tgtcgacgcc catcgcgtcc gcctcgcgcc acgcttgacg
     3781 caagacgtcc atcgtcgtgt gctgcggacg gatctggact ccaaccttga aacgcgccac
     3841 gaggtcacct ccacggccac ggcgaggatc gtcgcgcctc cagtgacgct atcgcgaagc
     3901 ccagcgaacg acggcccgca gctcaggctg aggcggtcag ccgaccttga tgacggcttc
     3961 ggccgaccag aaggcctcgc ctccgtcgag cgaccgcatc ctcgcgtgga accggtactt
     4021 gcccttgccg gcgttcgggg tgaacgaggt cttgcgacca accacgccgc tgcgccagtc
     4081 cgcccagtcc ttgcccggct tctggatctg cacgtcgtag acgaaccctt cgggcgccgc
     4141 gatggaggcc cacgtcagat ggaacgttcg atgagggccg ccgctggccg gactgacgcg
     4201 catggggatc gccacacggc cgcccatctc cacgtgcggc gtgcaggtga atcgatagac
     4261 acccgcggcc gggaacgtgt accacgtgct gggctcgccg gctcccaccg agcccgagtc
     4321 gtacagctcc atcccgctgc tgtcggtggc cgtgtgatgt gacggcccca cgaagtcgaa
     4381 caccaccgtc tgtccacgtc ccaacggcac cgtggccgga tcgaacagga agtccgacac
     4441 ctcgacgtgg acgccgtcgg gcggtggagt tcccggcgac gttcccggct gcccggcacc
     4501 cgcgggcagc ccgccgctca cccccagggt gaagacgagc agggcagcgg gaacgaggag
     4561 caaccggtgt cgcacccttt ccctatcggc caccggcgcc gattcccgaa ccccggcacc
     4621 tacgattggc gcgatgagcg cgaccggtga acgcatcggc accacccgcg atgcgctccg
     4681 ccacgccgat ttccggcgat tgttctcgat ccggctgatc agccagagcg ccgacgggtt
     4741 ggtccaggcg gcgctcgtgg cctcgctcgc gttctcgccc gaacgcgcca ccaccgccag
     4801 cgggttcgcg atcgcctcgg cgatcgtgat catcccctac tcgctgctcg gcccgttcgc
     4861 cggggtgttc atcgatcgct ggtcgcgacg caagatcctg gtgatcgcgc cgttggtgcg
     4921 cgcggcaccg gcgttcctga tcctggcgag cccgacgcgc tacgccttct ggtactacgc
     4981 cggcgcactc ttcgtgacga gcgtgaaccg gttcttcctg gcgaccgccc aggcggtggt
     5041 gccgcggatg gtgcccacca aggatctgct tgccgccaac ggcatcgcca cggtgggcgg
     5101 cacggtggcg ctgctcgtcg gcgtgttcgc cggcggcctg atctccgacg cgttcggcaa
     5161 cggcgcgatc gtgacggcgg ccgcgatcat gtggctcgtc acgtcgttga tcgcctggcg
     5221 catccgcagc gacctgcgcc cccacctgct gcctgagtcc tccgacctgc tgcgccatca
     5281 gctgcggcgg gtggggttcg agttcgtcga cgggatcacg cacatcgtgc gcacgccgcg
     5341 cgcgatcgcg ccgatcacct cgatcgcggt cgaccagatg ggccaaggcc tggtgttggt
     5401 gctggcgttg gtggtgttcc gcgaacggtt cggcaccggg gtgggctcgt tctcatggtt
     5461 gatcggcgcg gggggcgtcg gcgtgttcct ggggctggcc accgtgggtt tgctcgaccg
     5521 gcggttctcg cgcgaacgca tcgtggcctt ctcgttcgcg ctcggaggcg tggccttgct
     5581 gttggtcgcc ctcttcctga accgctggtc ggcgttgctg gcgagcttcg tggtgggact
     5641 gacgttcgcg tggaagaagg tgccgatcga cacgatggtg caggaggccg ttcccgacgg
     5701 gttgcgcggt cgtgtgttcg ccgtctacga cgtggcctac aacctcgcgc gactgacggc
     5761 ggcgttgctc gcggtggtgc tgctgcccgc gatcggtgag aacggcgcgg cggccgtggt
     5821 cggcatcgtg ttcctgttgt gggtgccggt gctgccgagg tggctccgca acgttcccga
     5881 gatcgtcgtg cgcgtgaacg ccgacggcga gcccgagcag gtgcaatggg gcggcgtcag
     5941 cgaacccgtc acggtcttgg acgcgcaagg atccggccgg tggcgcgtgc ggctggccga
     6001 cggcaccatc ctggcgatcg gcaccctcgc cgacggccgg tgggagatcg ccgaagagat
     6061 cgagccctag gcgaaccacc gatcgagcgg aacgtcgacc tcgagcgacg agaagtcgcc
     6121 ggtctccagg agcgcggtga cggcgtcggc catggccgcg acagcgaccc aggtcaggcc
     6181 accaccgacg ctcacacgct gggcgcctgc ctcgaagatc tcgccgatcg tgaacccggg
     6241 gaacccgagc acgttgaccg ggcgcgacac cgcggaacac accgcacgga tctcgtccac
     6301 ggtcgccagg cccggggcgt acagcacgtc ggcgccggcg gcctcgtagg cctggagacg
     6361 cgcgatcgtg tcgcccaggt cggggttgtc gcggatgtgg ttctccgcgc gccccgtaag
     6421 catgaacgga acgtcgagcg cgcgagccgc ttcggcggcg gccgcgatcc gatcagcggc
     6481 gtggccgacc tcgtagaggc gaccgccgtc gcgatcgaag tcctcgatcg agccacccac
     6541 cgcgcccgcc gctgcgaccc gggtgatcgc gagcgccgcc tgctcgggct cggggccgta
     6601 cccgttctcg aggtcgaccg acaccggcac ctcggtcgcc gcggtcaccg acgccgtgtg
     6661 ggccatcgcc tcgtcgggcg tgacctcccc gtcccggcgc ctgagcgtga acgcgaaccc
     6721 ggagctggtg gtggcgagcg ccttgcatcc cagcgcctgc atcacccggg ccgaaccggc
     6781 gtcccagggg ttggggatga cgaacggctc gccggcatgc agcgcgcgga acgcctcgcc
     6841 cttcgctcgt tgatcagaca tcgtcaccgt cctcactgac gcggagcacc accttgccac
     6901 gcttgccgct ggcctgagcc cgttcgaacg ccgaccgcgc gtcggcgagc gggaagaccg
     6961 agtcgatcgc aggaaccagg tcgcccgtct cggcgagccg ggtcagttcg accagttgct
     7021 cgcgattcgg ttcgaccacg aagtacgacg tctcgatctt cgcgtcggct gggatctccg
     7081 gggtctcctc ggccaccgac accagccgcc cgcccggccg gatcacggcc ggcgaagctt
     7141 cgagggtcgc cccgcccccg gtgtcgaaca ccagatcgac cggctcgagc ggcctcagat
     7201 ccgaaccgtc gatcacctca tgggcaccga atcctcgcgc cgcttcctgg ctcgctggcg
     7261 acgccgtgcc gatcacgtgc gcgccacggt gccgcgcgag ttgggtcgcc aggtgaccga
     7321 cgccgccggc cgcgccggtg atcaggaccc gctgtcccgc ctccaaaccg ccgtgatcga
     7381 acagcccctg ccacgccgac agccccggga gcgggatcgc cgcgctctgg acgtcgtcga
     7441 gggtgcgggg tttcggggcc aggagttccg ccgccaccgc gacgtagtcg gcggcggccc
     7501 cgtcccggcc gaagtcggtg agcgcgtaca ccgagtcacc gaccgagacc gtctcgacat
     7561 cctgcgccac ggccgcgacc gtccccgaga cctcgtagga agggatggcg gggagccgat
     7621 cctccggcca ctccagctcg tccttcgtga gggctgccgc atgcacccgg atcagcgctt
     7681 cgcccggtgc gatgtccggg acggagatct cctcgagctc cagcgctccc ggtccgccgg
     7741 gagacgacag cctgatggca cgcatcgtgg gatcgatcgc cgtgtccgat gcttcgtggt
     7801 cggtcatggg tcctcctcag aggaacaatc ttgcttcctg ataagtatct tactaggaag
     7861 tatactgtcg gtagagatgc agcactcctc accggaccac gtcgacgaga tcctcgcgca
     7921 gtggcagcgc gagcgtcccg acctggatgc gagcccgatc ggcgtgttcg ggcgactctc
     7981 acgggctgat cgcatggtcg acaccgcgct cgcagcaggg atcgctgcac acgggctcca
     8041 gcccggctgg ttcgatctgt tggccgcgct gcgccgccag ggtgctccgt acgagctcac
     8101 ccccacgagg ctgacggagg cgaccctgct ctcatctggg ggcaccacca agcggctgga
     8161 tcgcctagcg gaagcggggc tgatcgaacg cctccccgat cccgacgatc gtcgtggcac
     8221 cctcgtgcgg ctcacacggc agggcaagcg agtgatcgac cgggcgctgc aaacccacct
     8281 cgagaacgag cggcggctgc tccaggtctt cagccgcgac cagcggcgta cgttcgagga
     8341 gctccttcga acactgctcg atggcctcga cgccgccgat cccccggcgt agcggcgtgg
     8401 atcagcgcct tcccgtacgt tcgaaccccc tcgaacccct acgacacgct acgtagtcct
     8461 gcttacttag cacgtggtag ccaggttccc catggcgtag gtattcacgc ggatgccgga
     8521 cacgtccacc atgtcccaag taccaccgag cacgactcgg ccgggaagga cggggaacat
     8581 gagcaagcac ggagagcacc aaccggggac cgtgggcacc tgcctgaagt gcacgaggat
     8641 cgcctacgaa cccagctggt acgccgtgat gctcgccttc gaggagcgga acctcgacac
     8701 cgtcgtccaa ctcacggatc acgccgaggc agtcacgacc cgatcgggtc acatctggac
     8761 gtagcccggg atccccctcg cgggctcacg gaggtcgaag gcggccggga acgcggttca
     8821 ccgactcccg cgccgccttc gacctcatct cttcccggtc ttccggacgg tcttcgcggt
     8881 tatcgcgacg cgatcgccga ggcagcgcct gcgtcgacgg cttcctcgtc ttcaccgaga
     8941 cgcccgacgc gagcgtgccg gcgtcccaga cccagcaacg ccacgccgag accggccgca
     9001 accaacccgc tgagccacag gcccgtctgc gcacccaggt gttcgccgac cacgccggcg
     9061 atgggggcgc cgatcggcgt cgagcccagg aagacgatcc cgtacaacgc catcacgcga
     9121 ccgcgcgcct gcggcaccga ggtcagctgc agcatcgtgt tgcccgtgat catgaacgcc
     9181 atgatcgtga accccatcgg gatcatcgcg agagccgcga gccacaagac gggcgccgca
     9241 cccgccaacg ccagcgacac cccgcacgcc accgcccacc atgcgaggtc acggagtttc
     9301 ggagcggcga tgcggctcgc catcgtgatc gctcccagga acgagcccac ccctgccagc
     9361 gccgacagca tgccgaacgt gccggcgtcc ccgtggaacg tccgctcggc gagcagcggc
     9421 acgaacaccg cgaagttgaa cgcgaacgtg aacacgacgg ccatggccaa caggggccgg
     9481 cgcaggtcgt cggttcgcca cacgtacttc agacccgcca ccaagtgacc gcggtcgcgc
     9541 gtgctcttct cctggcgatg gagctcgctg gggcgcatca tcagcagcgc ggccagcacc
     9601 gcgatgtagg agaacgcgtc gaccaggaag caggtggcga tccccacggt agcgatcagg
     9661 acccccgcga tggcgggacc gatcacacgt gacccggtga acgccgcgct gttgaggctc
     9721 acggcgttgc gcacttgctc ttcgcccacc atctccacgt agaagctctg ccgcgaaggg
     9781 ttgtcgaacg cggtgaccag acccgaggcg agcgacagga cgtagaccat ccacagttgc
     9841 gcgacgtcgg tggccacgag cgaccacaac gtgaatgcga cgaccgcgta ggccgcctgg
     9901 gtgacgagca ggatcttgcg cttgtcgaac cggtcggcga gcacgccgcc gtacgcgccc
     9961 agcagcagca ccggcaggaa gtagagcgcg atgttcgtac ccaaggcggt gccgctgtgg
    10021 gtcagctgca gcaccaacca cgccgacgcg gtgaagttca tccaggtgcc gatcgcggac
    10081 accgtctgac cgaacaggta gagccggaag ttgcgcgaac tcttgaccga ggacagcgtc
    10141 cgccccaggg cttcggtgat cttggagctc atacgggccg ctccgtgatg cgatccagga
    10201 tcagggcagc acgctcgagc gtctgcaact cgtcgggagg cagccgcttg atctttcgcg
    10261 cgaggtactc ggcgtgccgg cgccgaaccc gcgacaggag cttccgtccg gcaggggtga
    10321 cctggatcca ggcaacccgg ccgtccagcg ggtcgggggt tcgttcgacc agcccgcgtt
    10381 ccacgag
//
LOCUS       DATDBZ010000357         2117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2575701, whole genome shotgun sequence.
ACCESSION   DATDBZ010000357 DATDBZ010000000
VERSION     DATDBZ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2575701"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VIX62_08670"
     CDS             <1..800
                     /locus_tag="VIX62_08670"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY5860342.1"
                     /translation="VLLAWQFESQPNRQVQAVAGEQVSGGNMEGKEVRFGVPAGAAFA
                     ASTTGTSTGSVNSAHDSMNPLGGGTVLVNIMLSEVSPGGVGSGLYGILVLAILAVFIA
                     GLMVGRTPEYLGKKIEPREMKLVSVYILLVPAMILILTGAAVVMDSAVASIFNPGAHG
                     LSEILYAFTSGANNNGSAFGGLSANTPFFNTAIGFAMLFGRFASIVLVLAVAGSLARK
                     KHVPESTGTFPTTSPLFVGLLTGVILIVTALTYFPALSLGPIVEGLS"
     gene            801..>2117
                     /gene="kdpB"
                     /locus_tag="VIX62_08675"
     CDS             801..>2117
                     /gene="kdpB"
                     /locus_tag="VIX62_08675"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008734574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY5860343.1"
                     /translation="MFDLSLLWRSLPDAFRKLDPRVMWRNPVMFVVEIGSVLTTLLFV
                     RDPSSFAGLIAVWLWLTVIFANLAEAVAEGRGKAQAAALRATRTETMARKLLADGTEG
                     SVPGTELQPGDRVIVEPNEVIPGDGEVVEGVASVDESAITGESAPVIRESGGDRSAVT
                     GGTMVLSDRIVVEITSKPGETFIDRMIALVEGAQRQKTPNEIALNILLASLTIIFLLA
                     TVTLQPIAIYSGAKQSVIVLVALLVCHIPTTIGALLSAIGIAGMDRLVQRNVLAMSGR
                     AVEAAGDVNTVLLDKTGTITLGNRQAAEFLPAPGVTVEALADAAQITSLADETPEGRS
                     IVVLAKEKYGLRGRDLEGADFIPFTAQTRMSGVDLDGRRLRKGASDSVRSWVQDQGGT
                     VSEEVDTIVAGIAVQGGTPLTVAENDRVLGVIYLKDIVKEGMRQRFD"
BASE COUNT          334 a          681 c          720 g          382 t
ORIGIN      
        1 cggtgctcct ggcgtggcag ttcgagtctc agcccaaccg tcaggtgcaa gcggtggccg
       61 gtgagcaggt cagcggcggc aacatggaag gcaaggaggt ccggttcggg gtgcccgcgg
      121 gcgctgcctt cgccgcgtca accacgggga cctcgaccgg ttcagtcaat agcgcccacg
      181 attcgatgaa cccgctgggc ggtggcacgg tcctggtcaa catcatgttg tcggaggtct
      241 cgccgggtgg tgtgggctcg gggctctacg gcatcctcgt gttggcgatc ctcgcggtct
      301 tcatcgcagg actcatggtg ggtcgtacac ccgaatacct cgggaagaag atcgaaccgc
      361 gcgagatgaa gctggtctcg gtctacatcc tgctcgtgcc ggcgatgatc ctgatcctca
      421 ccggcgctgc ggtggtgatg gactcggcgg tcgcgtcgat cttcaacccg ggtgcgcacg
      481 gcttgtccga gatcctgtac gcgttcacgt cgggagccaa caacaacggc agcgccttcg
      541 gcgggttgtc ggccaacacc cccttcttca acaccgcgat cggtttcgcg atgctgttcg
      601 gacggttcgc ctcgatcgtg ctggtgctcg ccgtcgcggg atccttggcg cgcaagaagc
      661 acgtccccga gtcgacgggc acgttcccca ccacgtcgcc gttgttcgtc gggttgctca
      721 cgggcgtgat cctcatcgtc accgccctca cgtacttccc ggcgctgtcg ctgggcccga
      781 tcgtggaagg actgtcctag atgttcgacc tctccctgtt gtggcgttcg ctgcccgatg
      841 cgttccgcaa gctcgatcca cgggtgatgt ggcgcaaccc ggtgatgttc gtggtggaga
      901 tcgggtcggt gctgaccacc ctgctgttcg tccgcgatcc gtcgtcgttc gccggcctca
      961 tcgctgtgtg gttgtggttg acggtcatct tcgcgaacct tgccgaagcg gttgcggaag
     1021 gccgaggcaa ggcgcaggca gcggcgttgc gcgcaacccg caccgagacc atggcccgca
     1081 aactcctcgc cgatggcacc gagggatccg tgcccggcac cgagctgcaa cccggcgatc
     1141 gcgtgatcgt cgaaccgaac gaggtgatcc ccggtgacgg cgaggtcgtc gaaggcgtcg
     1201 cctcggtcga tgaatcggcc atcaccgggg aatcggcgcc ggtgatccgc gaatccggcg
     1261 gcgaccggag tgccgtcacg ggcggcacca tggtgctgtc ggaccgtatc gtggttgaga
     1321 tcacctcgaa gccgggggag accttcatcg atcgcatgat cgccttggtc gagggcgcgc
     1381 agcgccagaa gacccccaac gagatcgcgc tcaacatcct gctcgcgagc ctcacgatca
     1441 tcttcctgct ggcgacggtg accctgcaac ccatcgcgat ctattcggga gcgaagcagt
     1501 cggtgatcgt cctggtggcg ctcctcgtct gtcacatccc gacgacgatc ggcgccttgc
     1561 tgtcggcgat cggcatcgcc ggcatggacc gattggtgca gcggaacgtg ctcgccatgt
     1621 cgggccgagc ggtcgaggcc gccggcgacg tcaacacggt gctgctcgac aagaccggca
     1681 cgatcaccct cgggaaccgc caggcagccg agttcctgcc agcccctggg gtcacggtgg
     1741 aggcgctcgc cgacgccgcg cagatcacga gcctcgccga cgagacgccc gagggacgtt
     1801 cgatcgtggt gttggccaag gagaagtacg ggctccgcgg ccgggatctc gaaggcgccg
     1861 atttcatccc gttcaccgcg cagacccgca tgagtggcgt cgacctggat gggaggcgtt
     1921 tgcggaaggg ggcgtccgac tcggtccgat catgggtgca ggaccagggc ggcaccgtgt
     1981 ccgaggaggt cgacacgatc gtcgccggga tcgccgtcca gggcgggacg ccgctcaccg
     2041 tcgccgagaa cgaccgcgtg ctcggcgtga tctacctgaa ggacatcgtg aaggaaggca
     2101 tgcggcagcg cttcgac
//
LOCUS       DATDBZ010000358         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_940641, whole genome shotgun sequence.
ACCESSION   DATDBZ010000358 DATDBZ010000000
VERSION     DATDBZ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940641"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VIX62_08680"
     CDS             complement(<1..329)
                     /locus_tag="VIX62_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5860344.1"
                     /translation="MKHLLVTNDFPPKIGGIQSLLWEWWRRLPADRFAVLTSPYRGSA
                     AWDAEQPFRVERIREPVLLPHPLMSRRADRLANEIGADLIVIDPAVPLGLIGPSLALP
                     YAVVLHGA"
     gene            complement(326..1447)
                     /gene="queG"
                     /locus_tag="VIX62_08685"
     CDS             complement(326..1447)
                     /gene="queG"
                     /locus_tag="VIX62_08685"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY5860345.1"
                     /translation="MPPLALDESEIAGLLEVGRANGLDALGIAAASTLDRARAALFER
                     RRRGLHAGMAFTYRNPERSTEPSAAVAGARSIIVGARRYLCDEPVRPIDPSARIARYA
                     WIDHYAPLRTGLWAMAHELRRAGWKAVAFADDNSIVDREVAWRAGIGWFGKNANLLLP
                     HAGSWFVLGCVITDAPLPVAAMPVADGCGSCRRCVDACPTGAIIESGVIDSNRCLAWL
                     LQKPGVFPREYRVALGDRLYGCDDCQEVCPPNVRFAASRGELGPLAEAWVSIAELLES
                     DDDTLLARHARFYIAGRDPRWLRRNALIVLGNIGDGADRRTGALLEHHLGHPDPILRA
                     HAVWAAARLARNDLLPAADPDPMVDAELNHLPEPRPASR"
     gene            complement(1484..1723)
                     /locus_tag="VIX62_08690"
     CDS             complement(1484..1723)
                     /locus_tag="VIX62_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860346.1"
                     /translation="MPLHPAAGHEDLPAATTLTISCDRCSMRATSACDDCIVTYICER
                     EPDDSVVFDLAEQRAVRLLVQAGLVPTLRHRVAGL"
     gene            1869..>2075
                     /locus_tag="VIX62_08695"
     CDS             1869..>2075
                     /locus_tag="VIX62_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860347.1"
                     /translation="MPISSQLPLIESKLVAPLLPPEITRRSRVLDRMSGEERVLLVVA
                     PIGYGKSVVAQQWLDESARDTAWLS"
BASE COUNT          330 a          725 c          718 g          302 t
ORIGIN      
        1 gccccgtgca gcacgacggc atacggcagc gccagcgacg ggccgatcag cccgagcggc
       61 acggcaggat cgatcacgat gaggtcggca ccgatctcgt tggcgagccg gtcggcgcgg
      121 cgcgacatga gcgggtgggg caacaggacc ggctccctga tccgctccac ccgaaagggc
      181 tgttcggcat cccatgccgc gctgccgcga taggggctcg tgagcaccgc gaaccggtcg
      241 gcggggagac gtcgccacca ctcccagagg agcgattgaa tgccgccgat cttcggtggg
      301 aagtcgttcg tgacgaggag gtgcttcatc gggatgcggg tcggggttcc gggagatggt
      361 tgagctcggc gtcgaccatc ggatcgggat cggcggcggg caggagatcg ttgcgggcaa
      421 gacgggccgc cgcccacacc gcgtgcgccc ggaggattgg gtcgggatga ccgaggtggt
      481 gctccaggag cgcgccggtt ctccggtcgg ccccatcgcc gatgttgccg agcacgatca
      541 acgcgttgcg gcgaagccat cgcgggtcgc gcccggcgat gtagaaccgt gcgtgccggg
      601 ccagcaatgt gtcgtcgtcc gactcgagca gctcggcgat gctcacccac gcctccgcga
      661 gtgggccgag ctctcctcgg gacgcggcga agcggacgtt cggggggcac acctcctggc
      721 agtcgtcgca gccgtacagg cggtccccga gcgccacccg gtactcgcgt gggaacaccc
      781 ccggtttctg cagcagccag gcgaggcatc ggttgctgtc gatcacgcca gattcgatga
      841 tcgctcccgt cgggcacgca tcgacgcagc gacggcacga gccgcagccg tcggccaccg
      901 gcattgcggc caccggcaag ggcgcgtccg tgattacaca gcccaagacg aaccagctgc
      961 cagcgtgtgg caacagcagg ttcgcgttct tcccgaacca accaatgccg gcacgccacg
     1021 ccacctcccg atccacgatc gagttgtcgt cggcgaacgc caccgctttc caccccgcac
     1081 ggcggagctc gtgcgccatc gcccacaacc ccgtgcgcag cggggcgtag tggtcgatcc
     1141 aggcgtaccg ggcaatgcga gcgctcgggt cgatcgggcg caccggctcg tcgcacaggt
     1201 atcggcgcgc cccgacgatg atcgaccggg cgcccgccac cgcggccgac ggttcggtcg
     1261 accgctccgg gttccgatag gtgaacgcca tgccggcgtg caacccacgc cgccgccgct
     1321 cgaacagcgc cgcccgtgcc cggtcgagcg tggacgctgc ggcgatgccg agtgcgtcga
     1381 gcccattggc gcgtcccacc tccagcaggc cggcgatctc ggactcgtcg agcgcgaggg
     1441 gtggcatcac catcgacggt accgctgccg ctcgcccgcc gcttcacagc cctgccaccc
     1501 gatggcgcaa cgtcggcacg agaccggctt gaacgagcag ccggacggct cgttgctcgg
     1561 ccaggtcgaa gacgaccgag tcatcgggct cgcgctcgca gatgtaggtc acgatgcagt
     1621 cgtcacaggc cgacgttgcc cgcatcgagc agcgatcgca gctgatggtg agggtggtgg
     1681 ccgccgggag atcctcgtgg ccggcagcag ggtggagtgg catgcccgca gcttgcacaa
     1741 ggggtgtgcc aacgttccga cgtgcgccgt gctgcgagtc gccacccgat ggaccgactc
     1801 gatcagcggc acccgatcca gtgtccgacc aactcgtcgg ggtaccctca ccggttgtgc
     1861 tcgcgggcgt gccgatctca tcgcagctgc cgctcatcga gagcaagctc gtcgctccgc
     1921 tgctcccgcc cgagatcacc cgtcgcagcc gcgtcctcga ccgaatgtcg ggcgaggaac
     1981 gagtcctcct cgttgtcgcg ccgatcggat acgggaaatc ggtcgtcgcc caacagtggc
     2041 tcgacgagtc ggcccgcgac acggcctggc tgtct
//
LOCUS       DATDBZ010000359         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1963980, whole genome shotgun sequence.
ACCESSION   DATDBZ010000359 DATDBZ010000000
VERSION     DATDBZ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963980"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VIX62_08700"
     CDS             complement(<1..1630)
                     /locus_tag="VIX62_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY5860348.1"
                     /translation="MTTVPPSMFGQPALRSEDPRFLRGRGRYLENLPIEGALRAVFVR
                     SMMPHARVLGVDVDAARSMPGVEAVFTADDLAIGPMPSSGNVESDEGGSIDDAFGREV
                     LARDVVRFVGEPLALVVAGSLATGMDAAETVLPDLEPLPAVTDVEAALADGAPLLWPD
                     RDSNVASTFTVRWDDDVLAGSDVIARGRFVNQRLAPVPMEPNAIAVEPDGEGGFTVWV
                     STQVPFDVRDDLAELLKVPKQKVRTIAPDVGGGFGAKLQIYPEYQAVAVAAARLGRPI
                     RWVETRSESMLGLTHGRAQVQLVEIGAKHDGTIVGLRADLIADMGAYPIGAFLPTTTQ
                     EMLSGVYTIRDIACRGRSVVTNATPVAPYRGAGRPEATALIERGIDLVAAELGMDPVV
                     VRRKNMIPPDVFPYETPSGTVYDIGDYERSLDLALELAGIDELRAEQSARRARGDHAE
                     LGIGVCTYVEITSFMSKEFGAVQVHEDGSITVETGTSPHGQGHETAFAQITSAVLGVP
                     FESITVVHSDTGRVRRGAGTWGSRSLQAGGSSVLE"
     gene            1705..>2284
                     /locus_tag="VIX62_08705"
     CDS             1705..>2284
                     /locus_tag="VIX62_08705"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY5860349.1"
                     /translation="MVGVFLAALMITTGSANAAVKVVQVAPLTNPVGFTFAPNGRIVY
                     AERDSGEIRFLNPRTGVDRLFFRIGGVNSEGERGTLGVALHPDWPRRPFVYVYVTRGP
                     GSAPLRNQLVRIRSVDGHGRDLRVLLQSPIGGRTNHNGGRIAFGPDGKLYVVIGDGGE
                     DPSTAQDLSDEPRGKVLRLNPDGSIPATNPFGA"
BASE COUNT          374 a          807 c          752 g          351 t
ORIGIN      
        1 gttccagcac cgatgacccg cctgcctgca gcgaccgcga gccccacgtg cccgcgccgc
       61 ggcgcacacg accggtgtca ctgtgcacca ccgtgatcga ctcgaacggc acgcccagca
      121 ccgccgaggt gatctgtgcg aacgcggtct cgtgaccctg gccgtgcggc gaggtgccgg
      181 tctccaccgt gatcgacccg tcctcgtgca cctgcaccgc gccgaattcc ttgctcatga
      241 acgacgtgat ctcgacatag gtgcagacac cgataccgag ttcggcgtgg tcgccccggg
      301 ctcggcgggc ggactgctcg gctcggagtt cgtcgatacc ggcaagctcc agcgcgaggt
      361 ccaaggaccg ctcgtagtcg ccgatgtcgt agaccgtgcc cgagggagtc tcgtacggga
      421 acacgtcggg cgggatcatg ttcttgcggc gaaccactac cgggtccatc cctagctcgg
      481 ccgcgaccag gtcgatcccg cgttcgatca gggccgtcgc ttcgggcctg cccgcgccgc
      541 ggtagggagc caccggtgtc gcgttcgtca ccaccgaccg gccccggcac gcgatgtcgc
      601 ggatggtgta gacgccgctg agcatctcct gggtcgtggt cggcaggaac gccccgatcg
      661 ggtaggcgcc catgtcggcg atcagatcgg cgcgcagacc gacgatcgtg ccgtcgtgct
      721 tcgccccgat ctccaccagt tgcacctgcg cccggccgtg cgtgaggccg agcatcgact
      781 ccgaccgtgt ctccacccag cggatcggcc ggcctaggcg cgccgccgcg accgccaccg
      841 cttgatactc cgggtagatc tggagcttcg cgccgaagcc cccacccacg tcgggcgcga
      901 tcgtgcgcac cttctgcttc ggcaccttca gcaactcggc gaggtcgtcg cgcacgtcga
      961 acggcacctg cgtcgagacc cagacggtga acccgccttc accgtcgggc tcgacggcga
     1021 tcgcgttcgg ttccatgggc accggggcga ggcgctggtt cacgaagcgc ccgcgcgcga
     1081 tcacgtcgga accagccagc acatcgtcgt cccagcgcac cgtgaaggtc gacgcgacgt
     1141 tggagtcccg gtcgggccac agcagtggcg cgccgtcggc gagggccgct tccacgtcgg
     1201 tgacggcggg caacggttcc aggtcgggca ggacggtttc ggcggcgtcc atgccggtcg
     1261 cgagtgaacc ggccaccaca agggcgagcg gttctccgac gaacctcacc acgtcacggg
     1321 cgagtacctc tcggccgaac gcatcgtcga tcgacccacc ttcatcgctc tcgacgttgc
     1381 cactcgatgg catcggcccg atcgctagat cgtctgcggt gaagaccgct tcgacgcccg
     1441 gcatcgagcg tgcggcatcc acgtccacac ccaacacccg ggcgtgcggc atcatggaac
     1501 gcacgaatac ggcccgaagc gcaccctcga tcggcaggtt ctccaggtag cggccacgac
     1561 cacggaggaa acgagggtct tcagaacgga gggccggttg gccgaacatc gagggaggca
     1621 ccgtcgtcac ggggcaatgc tcccagatgg tgctcaggag ccgagagggg atgcgagctg
     1681 tcgcaccgaa ggaacagcac catcgtggtc ggcgtgttcc ttgctgccct gatgatcacg
     1741 accggctcgg cgaacgccgc ggtcaaggtg gtccaggtgg cgccgttgac caacccggtg
     1801 gggttcacgt tcgcaccgaa cggccgcatc gtgtatgccg aacgtgacag cggcgagatc
     1861 cgcttcctca accccaggac aggtgtcgac cgcctcttct tccggatcgg tggcgtgaac
     1921 agcgaaggag agcgcggcac gctgggcgtc gcgctccacc ccgactggcc gcgccgcccg
     1981 ttcgtctacg tctacgtgac gcgaggtcct ggttcggcgc cgttgcggaa ccaactggtg
     2041 cgcatccgca gcgtcgacgg ccacggccgc gatctacggg tgctgctgca gtcaccgatc
     2101 ggcggaagaa ccaaccacaa cggggggcgg atcgcgttcg gacccgacgg caagctctac
     2161 gtggtgatcg gtgacggcgg tgaggatccc tcgaccgcgc aggacctgtc cgacgaaccg
     2221 cgcggcaaag tccttcgcct caaccccgac ggctcgatcc ctgcgaccaa cccgttcggg
     2281 gcca
//
LOCUS       DATDBZ010000360         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2347626, whole genome shotgun sequence.
ACCESSION   DATDBZ010000360 DATDBZ010000000
VERSION     DATDBZ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347626"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VIX62_08710"
     CDS             complement(<1..1190)
                     /locus_tag="VIX62_08710"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY5860350.1"
                     /translation="MASTTTNERLQAWVADWAAVLQPDEIYWCDGSAEEYDRLAGELV
                     TSGTFTKLDDAKRPNSYWAHSDPGDVARVEDRTYICSKHEGDAGPNNNWREPAEMRAE
                     LTKLYTGAMRGRTMYVVPFSMGPLGSPIAHIGVQLTDAAYVAVSMRTMTRMGQPALDA
                     LGDREWVRCLHSVGAPLEDGAEDVPWPCNPDNKYIVHFPEDREIWSYGSGYGGNALLG
                     KKCFALRIASVMARDDGWLAEHMLILKLTSPDQDVKYVAAAFPSACGKTNLAMLVPTI
                     PGWTVETVGDDICWMKFGDDGRLYAINPEAGFFGVAPGTSDDTNPNAMATLWGNSIFT
                     NTALLPDGDVWWEGMTDEPPARAIDWRGNPWNPEADTPAAHPNARFTVSAAQCPSIAP
                     EWEDP"
     gene            complement(1261..1482)
                     /locus_tag="VIX62_08715"
     CDS             complement(1261..1482)
                     /locus_tag="VIX62_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860351.1"
                     /translation="MQGVIKAYDPGTGDGVVITDVGMVEYDLAQNALEGSIFRMVRQG
                     QRVIFELDADSRATRLRLGSEEDMATPGP"
     gene            complement(1499..1699)
                     /gene="rpmB"
                     /locus_tag="VIX62_08720"
     CDS             complement(1499..1699)
                     /gene="rpmB"
                     /locus_tag="VIX62_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010534859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY5860352.1"
                     /translation="MAAVCKLCGKHPSWGMSVSHSHRRTKRRWNPNIQRIRAIVNGSP
                     QRLHVCTGCIKAGKVTKVGAGR"
     gene            complement(1891..>2586)
                     /locus_tag="VIX62_08725"
     CDS             complement(1891..>2586)
                     /locus_tag="VIX62_08725"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5860353.1"
                     /translation="AGVSISWYTWLEQGRPINASRDVLDALARALRLDEVEHEHLIAL
                     ATRPPHRDLPPAVPTVPIGIAALLAALEPAPAYVLGPRWDVRAWNRAEARLYPPFDHL
                     GSDELNLVWVMFAEPTIRTLLGDWETEARQVLSLFRADTTPYREDRQIVALVERLTET
                     SPEFAAWWPRHDVAAFEERIRTFHHPIAGTLTFHNEQLVPAGEPDLRVVVHLPIDGDD
                     SAEQLRNVRHYIA"
BASE COUNT          404 a          855 c          891 g          436 t
ORIGIN      
        1 gggtcttccc actccggggc gatcgacggg cattgcgccg ctgacacggt gaagcgggcg
       61 ttcgggtgcg cggcaggcgt gtcggcctcg gggttccagg ggttgccgcg ccagtcgatc
      121 gcccgcgccg gcggctcgtc ggtcatgcct tcccaccaca catcgccatc gggcaggagt
      181 gcggtgttcg tgaagatcga gttgccccac aacgtggcca tcgcgttggg attggtgtcg
      241 tcgctggttc cgggagcgac accgaagaac ccagcttcgg ggttgatcgc gtagagccgg
      301 ccatcgtcgc cgaacttcat ccagcagatg tcgtcaccga ccgtctcgac ggtccacccc
      361 gggatcgtcg gcacgagcat cgccaggttc gtcttgccgc atgccgacgg gaaggcggcg
      421 gcgacgtact tcacgtcctg gtcgggcgag gtcagcttca ggatcagcat gtgctcggcc
      481 agccaaccgt cgtcgcgggc catcacggac gcgatgcgca gcgcgaagca cttcttgccg
      541 agcagcgcgt tgccgccgta ccccgacccg tacgaccaga tctcccgatc ctcggggaag
      601 tggacgatgt acttgttgtc agggttgcac ggccagggaa cgtcctcggc accgtcctcg
      661 agcggcgcgc cgaccgagtg gagacatctg acccactcac ggtcgccgag cgcgtcgagc
      721 gcgggctggc ccatgcgggt catggtccgc atgctcacgg cgacataggc cgcgtcggtg
      781 agctgcacgc cgatgtgtgc gatcggcgag ccgaggggac ccatcgagaa tggcacgacg
      841 tacatcgtgc ggccccgcat cgcaccggtg tagagcttgg tcagttcggc ccgcatctcc
      901 gccggctcgc gccagttgtt gttcggccct gcgtcgccct cgtgcttcga gcagatgtac
      961 gtgcggtcct ccacccgagc gacatcaccc ggatcggagt gggcccagta gctgttcggg
     1021 cgcttggcat cgtcgagctt cgtgaacgtg ccggaggtga cgagctcgcc ggccaatcgg
     1081 tcgtactcct cagccgaacc atcgcaccag tagatctcgt ctggctgcag gactgccgcc
     1141 cagtcggcga cccacgcctg aagccgttcg ttggttgtgg tggaagccat tgctgtactc
     1201 ctgcgggaag ggggcacacc gccgtgcccg aaccggggat ggaggcctgt cagctcgccg
     1261 tcatgggcca ggtgtcgcca tgtcttcctc tgaaccgagc cggaggcgcg tggcgcggct
     1321 gtcggcatcg agttcgaaga tgacgcgctg gccttggcgg accatgcgga agatggaacc
     1381 ctcgagcgcg ttctgagcga ggtcgtattc gaccatgccg acatccgtga tcacgacgcc
     1441 gtcgccggtg ccggggtcgt aggccttgat cactccctgc atggcggcgc cggtccgatc
     1501 agcgcccggc gccgactttg gtcaccttgc cggctttgat gcaacccgtg cacacgtgca
     1561 gccgctgggg gctgccgttc acgatcgccc gaatgcgctg gatgttgggg ttccagcggc
     1621 gcttcgtgcg gcggtgcgag tgggacacgg acatgcccca cgacgggtgc ttgccacaga
     1681 gtttgcatac tgctgccatc ggggcaccct tcgacatgac cggagacccc ggccggagaa
     1741 cagatagagg gtaccagcgg tcgtccaacg ccccaagcga aacgggccag caccgcgaca
     1801 gatttccctg atctccccat ctcgttcagt gatgggtgcg gacgtgggcc ggcggcggac
     1861 aaccggcggc ggtcagtccg cggcggtcga tcacgcgatg tagtgccgca cgttgcgcag
     1921 ctgctcggcg ctgtcatcgc catcgatcgg aaggtggacg acgacgcgca ggtcgggctc
     1981 ccctgcgggc accagctgct cgttgtggaa cgtcaaggtt cccgcgatcg gatggtggaa
     2041 cgtcctgatg cgctcctcga acgccgcgac atcgtggcgc ggccaccacg ccgcgaactc
     2101 cgggctcgtc tccgtcagcc gctcgacgag cgccacgatc tgccggtcct cccggtatgg
     2161 cgtcgtatcg gcccggaaca acgagaggac ctgtcgggcc tccgtctccc agtcgcccag
     2221 caacgtcctg atggtcggct ccgcgaacat cacccagacg aggttgagct cgtccgagcc
     2281 gaggtgatcg aacgggggat agaggcgagc ttcggcccgg ttccaggcgc ggacatccca
     2341 acgcggaccg aggacgtacg ccggggcggg ctccaacgcc gcgagaaggg ccgcgatccc
     2401 gatcggcacc gtcggcacgg caggcgggag atcccggtgc ggtggccggg tggcgagggc
     2461 gatcaggtgc tcgtgctcga cctcgtcgag acggagcgca cgcgccagtg catcgagtac
     2521 gtcacgcgac gcgttgatcg ggcggccctg ctcgagccac gtgtaccagc tgatcgagac
     2581 gccggc
//
LOCUS       DATDBZ010000361         4518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1965492, whole genome shotgun sequence.
ACCESSION   DATDBZ010000361 DATDBZ010000000
VERSION     DATDBZ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965492"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="VIX62_08730"
     CDS             complement(<1..1417)
                     /locus_tag="VIX62_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY5860354.1"
                     /translation="MATKTSPSSRRTASRTSASRKASSSGRKAAPKKKPASKKPPAKK
                     KPARASTRQILSPFARDAVGIALVVFAGLAVLSVWFDLAGPMGQAMTDLLQSGFGLLA
                     VAIPVIGVFWGFVLLRDVAPEDRVRMFIGFGLLTVGVLGMVSIARGNPGVFAPLEDTK
                     RAVGLGDAGGIIGAIGAGPLSKVVSPYGAFVIDAGLAAIGLLIFTGTSFAEAKQKVSD
                     FRSSLVEKDEVAKTAKEAKAAAKAEKAAAREAAPADAAAKPKEPKKRAKFMEAMGLVE
                     EPVVVLPESSPVLPLDEPPAAEEPGLPAKNAPKPRTVTTAAGPYELPSLDLLRTAPPS
                     TNDGIHETGVMEALAHTLTTFGVDAKVVDSHRGPTVTMYEVEVASGTKVNKVLNLSSD
                     IAYALATPDVRIIAPIPGRSAIGIEVPNKHRDFVMLGDILRSKAAKAATHPLQVALGK
                     DIHGEAQMVNLATMPHVLIAGA"
     gene            complement(1460..3091)
                     /locus_tag="VIX62_08735"
                     /pseudo
     CDS             complement(1460..3091)
                     /locus_tag="VIX62_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
     gene            complement(3120..4016)
                     /gene="dapA"
                     /locus_tag="VIX62_08740"
     CDS             complement(3120..4016)
                     /gene="dapA"
                     /locus_tag="VIX62_08740"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY5860355.1"
                     /translation="MAEHMSGNFGNVATAMITPFRADHGVDLDGAQELASWLVEHGTD
                     AVVVAGSTGESPTLTHKEKAQLFAAVGEAIRGRGKLICGTGTYSTAETLELTQAAEDA
                     GADGLLVVTPYYNKPPQRGLIAHFEHIADATELPMILYNIPGRTATRIEHDTLIELAQ
                     RPNIVAVKDSTGDFQAVSKLIAEAPAGFEVYSGDDWATFGYVCLGAVGVVSVAGHLVG
                     EQIRQMIDLIHTGDVPAARKIHEALSPLYNALFVTSNPIPVKAALEMQGRPAGPTRLP
                     LVPATAEERERVRKALEDAGLL"
     gene            4092..>4518
                     /locus_tag="VIX62_08745"
     CDS             4092..>4518
                     /locus_tag="VIX62_08745"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY5860356.1"
                     /translation="MPRIYTKTGDDGTTGLLYGGRVPKDDLVTEAYGTTDEAVAVLGL
                     ARSLTADEVMVADLLTLQRELFVVGADLATNPKERRKLKEGISLVNEAMVQRLEHRID
                     VLVAQRPLPEVFIVPGANPASASIDIARSTIRRAERAVVA"
BASE COUNT          696 a         1537 c         1570 g          715 t
ORIGIN      
        1 tggcccccgc gatcaacacg tgcggcatcg tcgcgaggtt caccatctgc gcctcgccgt
       61 ggatgtcttt gcccagggcc acctgcaagg ggtgggtggc cgccttcgcc gccttcgacc
      121 gcaggatgtc gcccagcatc acgaagtcgc ggtgcttgtt gggcacctcg ataccgatcg
      181 ctgaccggcc ggggatggga gcgatgatgc gcacgtcggg cgtggccagc gcgtaggcga
      241 tgtcgctgga caggttcaac accttgttga ccttcgtgcc ggaggcgacc tccacctcgt
      301 acatcgtgac cgtcggtccg cggtgagagt ccacgacctt cgcgtcgacg ccgaacgtgg
      361 tgagcgtgtg cgcgagcgct tccatcacac cggtctcgtg gatgccgtca ttggtggagg
      421 gcggcgcggt gcgcagcaga tcgagcgaag gcagttcgta cggacccgcg gcggtggtga
      481 ccgtgcgcgg cttgggcgcg ttcttcgccg ggagcccagg ctcctcggcg gcgggcggct
      541 cgtcgagcgg caacaccggt gaggattcag gcaggacgac gacgggctct tcgaccaggc
      601 ccatggcctc catgaacttc gcgcgcttct tgggttcctt gggcttggcg gccgcatcgg
      661 cgggcgcggc ttcacgggcg gcagccttct cggccttggc ggcggccttc gcttccttgg
      721 ccgtcttcgc gacctcgtcc ttctcgacca agctcgagcg gaagtccgac accttctgct
      781 tggcctctgc gaacgacgtg ccggtgaaga tgagcaggcc gatcgccgcc aggcccgcat
      841 cgatcacgaa ggcgccatag ggagagacca ccttggagag cggaccggcc ccgatcgcgc
      901 cgatgatgcc gccggcatcg cccaggccca ccgcacgctt cgtgtcttcg agcggcgcga
      961 acacccctgg gttcccacgc gcgatcgaca ccatgcccag cacgccgacc gtcagcaggc
     1021 cgaacccgat gaacatccga acgcggtcct ccggcgcaac gtcgcgcagc aggacgaacc
     1081 cccagaacac cccgatcacc gggatcgcca ccgcgagcag accgaagccg ctctgcagca
     1141 ggtcggtcat cgcctggccc atgggccccg ccaggtcgaa ccagaccgac aggaccgcca
     1201 gacccgcgaa taccaccaac gcgatcccga cggcgtcacg cgcgaacggc gacaggatct
     1261 gccgcgtgga ggcgcgtgcc ggcttcttct tggcaggggg cttcttggac gccggcttct
     1321 tcttcggcgc ggccttccga ccgctcgaag aagccttacg ggatgcggac gtgcgcgacg
     1381 cggtgcgtcg cgaagaaggg gaagtcttcg tcgccatagc ccataagcat gacgggttgc
     1441 ggcgacgctg caaccatcct cagacctcca tcacgaccgg gaggatcacg ggcttgcgct
     1501 gcgtggcctc gttgaagtag cgcccgagca cgcggcggat gtcctgctgg agggccgatg
     1561 ggtcgatcac cttggcgtcg gaggactcgg cgatcgcatc caccacgcgc tcacgcgcct
     1621 cgtcgaggat gccctgggag gtctcttcgt agacgaaccc gcggttcacg atgtcggggc
     1681 cgctcacgag ctcgcccgtc tgggagtcga ccgtgaccac cacgacgacc acgccgtcgc
     1741 ctgccagctt ccgccggtcg cgcagaacct cgccgcccac gtcgccgatg ccgaggccgt
     1801 cgacgaaggt catcccggcc tggacgcgct cgacgatctc gagccgctcc cccacgtcga
     1861 cgacgtcgcc gtcctcgcag atgatcacgt ggtcttcggg gatgccgacc tcctcggcga
     1921 gcctggcgtg gtgctgcagg tgccggcgct caccgtgcac cgggatgaac cagcgcgggc
     1981 gcaccagcga gagcatgagc cggagctcct cggcggcggc gtggccgctg gcgtgcaccg
     2041 ggtcggcggg catgtggtag acgtcggcgc cggtccggta cacggcgtcg accacccggt
     2101 ggatcgccgg ctcgttgccg gggatcaacg acgaggacag caccacggtg tcgcccgcct
     2161 tgagcttgac ccacttgtgc tgacgctggg ccatcagcga gagcgcgctg aagggctcgc
     2221 cctgcgaccc cgtacagatc acgacgacgc ggccgggatc gaggttcttg acctcttcga
     2281 tgtcgaccac gtcgcggtcg tcgacatgca acagccccag gagacgggcg gcgcgcaccg
     2341 agttgtgcat cgaccggccc aggaaggcca ccacgcgctc gttcgagacg gcggcgttca
     2401 cgatctgctg gatccggtgg atgtggctgg agaagcaagc cgccaccacg atctcgggcg
     2461 cgttggccac gatgcgctgc agcaccggac ccacgctctg ctcactcgag gtgtagccgg
     2521 cctcctccgc gttcgtggag tcggacagga gcaggtgcac gcccgtaccg ctggcctcct
     2581 cggccagacc gtgcaggtcg gtgggccgac cgtcgagcgg cgtctggtcg atcttgaagt
     2641 cgcccgtgtg caggatcgtg ccgaacggtg tgtcgatcgc cacggccata ccgtcgggga
     2701 tcgagtgcgt gacgcgtagg aagcgcatcg agaacgggcc ggcggtggca ccttcaccgg
     2761 gggtgacgac gtgcaggtcg gcccggtcgg tgacgccgtg ctcctcgagc ttgcccttca
     2821 agagttcgag cgtgaagggc gtgccgtaga tcggcagatg gtcgaactcg cggagcaggt
     2881 acggcaacgc tccgatgtgg tcttcgtgac cgtgggtgag cacgaccgct tcgacgcggt
     2941 cgacgttctt gcgcacgtag tcgaagtccg gcagcaccag gtcgatgccg ggcatgtcgc
     3001 ccgaggggaa cgacaggccg acgtcgacga tgaggatgcg tccgtcgacc tcgacgcacg
     3061 ccatgttgcg accgacctcc ccgacacctc cgaggaagac gacgcgacag aggttcgggt
     3121 cagaggagac cggcgtcctc cagagccttg cgcacgcgct cgcgttcttc ggccgtggcc
     3181 ggcaccagcg gcagccgcgt gggaccggcc gggcggccct gcatctccag ggcggccttc
     3241 accgggatgg ggttggaggt gacgaacaac gcgttgtaga gcggcgagag cgcctcgtgg
     3301 atcttgcggg ccgcagggac gtcaccggtg tggatcaggt cgatcatctg gcggatctgc
     3361 tcgcccacga ggtggccggc cacactcacc acgcccacgg cgcccaggca cacgtagccg
     3421 aacgtcgccc agtcgtcgcc cgagtagacc tcgaaccccg ccggggcctc ggcgatcagc
     3481 ttgctgaccg cttggaagtc accggtggag tccttcacgg ccacgatgtt gggccgctgc
     3541 gcgagctcga tcagcgtgtc gtgctcgatc cgcgtggcgg tccggccggg gatgttgtac
     3601 aggatcatgg gcagctcggt ggcgtcggcg atgtgctcga agtgcgcgat caggcctcgc
     3661 tgcgggggct tgttgtagta cggggtcacc acgagcaggc cgtcggcgcc ggcgtcctcg
     3721 gccgcctggg tgagctccag ggtctcggcc gtggagtagg tgcccgtgcc gcagatcagc
     3781 ttgccccggc cgcggatcgc ctcgcccacc gcggcgaaga gctgcgcttt ctccttgtgg
     3841 gtcaacgtgg gcgactcgcc cgtggaaccg gcgaccacga ccgcgtcggt gccgtgttcg
     3901 acgagccatg aggcgagttc ctgcgcaccg tccagatcga ccccgtggtc ggcgcggaac
     3961 ggcgtgatca tggcggtggc gacgttcccg aagttcccgc tcatgtgttc ggccatggtg
     4021 gtgcctccct ctctggccac cgcgaacgag ccggggtgcg gcggcctgga tgcgccctat
     4081 gctaacggcc catgccccgc atctacacca agaccggcga cgacggcacc accggcctgc
     4141 tctacggggg acgggtgccg aaggacgacc tggtcaccga ggcctacggc accaccgacg
     4201 aggcggtcgc cgtgctgggc ctggctcgct cgctcaccgc cgatgaggtc atggtcgccg
     4261 acctgctcac cctgcagcgg gagctgttcg tggtgggggc cgacctggcc accaatccca
     4321 aggagcgccg gaagctgaag gaaggcatct ccctggtgaa cgaggccatg gtgcagcgcc
     4381 tggagcaccg gatcgacgtg ctggtggccc agcgtccgct ccccgaggtg ttcatcgtgc
     4441 cgggtgcgaa cccggcgagc gcctcgatcg acatcgcgcg cagcacgatc cgacgggcag
     4501 aacgcgcggt cgtggccc
//
LOCUS       DATDBZ010000362         4180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2578163, whole genome shotgun sequence.
ACCESSION   DATDBZ010000362 DATDBZ010000000
VERSION     DATDBZ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2578163"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VIX62_08750"
     CDS             <1..1086
                     /locus_tag="VIX62_08750"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5860357.1"
                     /translation="CDSVVDSSAAAWRETGATSRSLDALLAESDVVSLHVPLTPQTRG
                     LLDAGRLELMKRGAMLVNTARGGIVDEDALAKAIESGHLGGAALDVFANEPTTDSPLF
                     ALDSVVVTPHLGASTKEAQDKAGTTVADMVRLALMGEFVPYAVNVSAGAEVTEVVRPF
                     VPLAERLGTLVAGLAEGGVRSIVASYLGRIAESDTRVLTLAILKGILGRTVHEPVSFV
                     NAPMLARERGLTVSEMRSTVSQDYVSSIALRAETDEGPVSVAGSILAKDHERVTQVND
                     FDIEVTPAGHMVFFTYADRPGIIGTVGTMLGEHHVNIATMDVGRRVEGGDALMCLTVD
                     TEVSIEVLEQVASIIDAHQLRTVTLPA"
     gene            1272..2519
                     /locus_tag="VIX62_08755"
     CDS             1272..2519
                     /locus_tag="VIX62_08755"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5860358.1"
                     /translation="MSRSPRSNVRRLALGRLLSVTGGAAAFTALNFTVWDRTHSPFMQ
                     ALSLLLTFGVAGLIGPFAGALGDRLDRRTVLIWSEAISCVFFTAMVFVHSPLALILLA
                     FASAIAELPFLSASRAAIPNLVDDPEDISWANSLVTLGVHAGIAIGPVIGGLLLALTD
                     VSWVFGVNAVSFVISLLLTISVRGRFQEERGAEHEEHAGITAGIVYLWREPVLRRLSI
                     AWFVFVVGMGMGMVADAPLAEDFGWGPMGYGLLIACWGLGSTVGAGVGRWMTARTEPV
                     WMVFGAFGIALAAFGVGFAPVFPFVLGALLAMGVCDGLTIVSENGMLQRRTPDAVRSR
                     AMAAFEAVLSFGLAVAYVAAGPVLTVMAPQSVYLLGGAGAALAALILVPLLRLRHEPD
                     KGSAPDLDAEADIADAMMYPPAS"
     gene            2728..>4180
                     /locus_tag="VIX62_08760"
     CDS             2728..>4180
                     /locus_tag="VIX62_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5860359.1"
                     /translation="MSGTTSSTGTNTYHAIVIGGGHNGLVAGAYLARSGAKTIVLEAR
                     HKVGGAADTMAPWPEAPEFKVTTLSYVMSLMPDTIQRDLKLADHGYKAYPVGPYFLPF
                     PDGRCIVQYDDDAKKNYESFAQFSKHDADAIERWDAWIGGLADVLGPMLMSIPPTVGS
                     KKPADLYEQLRLAWRFRGLDVKTIGDVTRLMTMSIVDILDRFFETDQVKTVMALNGLI
                     GTWAGPYEPGTGYVMAHHSIGDVGDGHLGAWAVPEGGMGAVSDAIAASARSFGAEIRT
                     DARVAKVLTEGGRVTGVVLEGGEELRAPVVVTAIHPKITFLEQIDRSELPAAFVDDID
                     NWKTRSGVVKINCALDRAPVFTAKPDLQDLTGGFELAHSITYLEKAFEEARAGLPSTA
                     PFSDGVMPTYYDRTLAPEGKHTVSLFTQWVPHEWSTAPHRDDLDAYADRIIDQVEAVA
                     PGFRDSILHRQVIGPYDMEHTYGLLGGNIFHGEL"
BASE COUNT          622 a         1387 c         1467 g          704 t
ORIGIN      
        1 tgcgactcgg tggtggattc atcggccgcg gcgtggcggg agacgggcgc gacctcgcgc
       61 tcactcgacg ccttgctcgc cgagagcgac gtcgtttcgc tgcatgtacc gctcacgccg
      121 cagacgcgcg gcctgctcga tgccggccgg ctcgagctga tgaagcgtgg ggcaatgctg
      181 gtgaacacgg cgcgcggcgg catcgtcgac gaggatgccc tggccaaggc gatcgagagc
      241 ggacacctgg gcggtgccgc gctcgacgtg ttcgccaacg agcccaccac cgactcgccg
      301 ctcttcgcgt tggacagcgt ggtggtgacg ccgcacctcg gagcctcgac caaggaggca
      361 caggacaaag ccggcaccac cgtggccgac atggtgcgac tggcgctgat gggtgagttc
      421 gtgccctacg cggtcaacgt cagcgccggc gccgaggtga ccgaggtggt tcgcccgttc
      481 gtgccgctgg ccgagcggtt gggcacgctg gtggcgggac tcgccgaagg tggtgtgcgg
      541 tcgatcgtgg cctcgtatct gggccgcatc gcggaatccg acacccgcgt gctcacgctg
      601 gcgatcctca aaggcatcct gggccgaacc gtgcacgagc cggtctcgtt cgtgaacgcg
      661 ccgatgctgg cacgcgagcg cggactcacg gtgagcgaga tgcgctcgac ggtgtcccag
      721 gactacgtga gttcgatcgc cctgcgagcc gagaccgacg agggcccggt gagcgtggcg
      781 ggttcgatcc tggcgaagga tcacgagcgc gtgacgcagg tgaacgactt cgacatcgag
      841 gtgacgcccg cgggccacat ggtgttcttc acctacgcgg accggccggg gatcatcggc
      901 accgtgggca cgatgctggg cgagcaccac gtcaacatcg cgacgatgga cgtcggccgc
      961 agggtcgaag gcggcgacgc gttgatgtgc ctcaccgtcg acaccgaggt ctccatcgag
     1021 gtgttggagc aggtggcgtc catcatcgat gcgcatcagc tgcgcaccgt cacgctgcct
     1081 gcgtagcagc gcccatcctt cgttcgttcc cggatcggcc tggggccccg tgctacactc
     1141 gctcgatcat gaagcgggat ctctcggtga ccggcgatgc cggcagtgtg cggacgcgat
     1201 ccatcgcgcc cactgcccgc taggtcacca ggcagccccg ccggcaccac cccccgcaag
     1261 gagtccgctt catgagccgc tcacctcgat ccaacgtcag acgtctggcc ctcggtcgcc
     1321 tgctgtctgt gaccggcggc gccgcggcgt tcacggcgct caacttcacg gtctgggacc
     1381 gcacgcactc gccgttcatg caggcgttgt cgctgctgtt gacgttcggg gtcgcggggt
     1441 tgatcgggcc gttcgccggt gccctgggcg accggctgga tcgccgcacg gtgctgatct
     1501 ggtccgaggc gatctcgtgc gtgttcttca ccgcgatggt gttcgtgcac tcgccgctcg
     1561 cgctgatcct gctggcgttc gcctcggcga tcgctgagct gccgttcctg tcggcctcac
     1621 gtgcggcgat cccgaacctg gtggacgacc cggaggacat ctcgtgggcg aacagcctgg
     1681 tcacgcttgg cgtgcatgca ggcatcgcga tcggccccgt gatcggtggg ctgctgctgg
     1741 ccctcacgga cgtttcatgg gtgttcgggg tgaacgcggt gtcgttcgtc atctcgctgc
     1801 tgctgacgat ctcggtgcgc ggtcgcttcc aggaggagcg aggcgccgag cacgaagagc
     1861 acgccggcat caccgcgggc atcgtctacc tgtggcgtga accggtgctg cgacggctgt
     1921 cgatcgcgtg gttcgtgttc gtggtcggca tggggatggg catggtggcc gacgcaccgc
     1981 ttgccgaaga cttcggctgg ggccccatgg gttacggatt gctgatcgct tgctggggat
     2041 tgggttcgac cgtcggggcc ggggtgggcc gatggatgac cgcgcgtacc gagccggtgt
     2101 ggatggtctt cggcgcgttc gggatcgcgt tggcggcgtt cggcgtgggg ttcgcgccgg
     2161 tgttcccgtt cgtgctgggg gcgctgctgg cgatgggtgt ctgcgacggc ctgacgatcg
     2221 tgtcggagaa cgggatgctc cagcgccgaa cgcccgacgc ggtgcggagc cgcgccatgg
     2281 cggcgttcga ggcggtgctg tcgttcgggc tcgcggtagc gtacgtggcc gcgggtcccg
     2341 tgctgaccgt gatggcaccg cagagcgtgt atctgctcgg tggggccggc gcggcgctgg
     2401 cggcgctgat cctggtgccg ctgcttcggt tgcgccatga gccggacaag gggagcgccc
     2461 ccgatctcga cgccgaggcc gatatcgccg acgcgatgat gtacccgccg gcgagctgag
     2521 gactcgaggg gcgactcgct ccctcgcccc ggcgatcgtc aacggcgtac gggttcccac
     2581 actgcatagg aaggtatctg ggtccgggcg gccgccaagc cagttgaatc acgttcaggt
     2641 ttcctgactt gctttgaatg gccgttcagg actagcgtcc cactccgggc gggtcccacc
     2701 cggggcccag accggaggaa caccgtcatg tccggcacca ccagctccac cggcaccaac
     2761 acgtaccacg cgatcgtgat cggcggcggc cacaacggcc tcgtcgccgg cgcctacctc
     2821 gccaggtcgg gcgcgaagac gatcgttctc gaagccaggc ataaagtggg cggtgccgcc
     2881 gacaccatgg ccccctggcc cgaggctccc gagttcaagg tcaccacgct cagctacgtg
     2941 atgagcctga tgcccgacac gatccagcgg gatctcaagc tcgccgacca cgggtacaag
     3001 gcctatccgg tcggcccgta cttcctgccc ttccccgacg ggcgctgcat cgtccagtac
     3061 gacgacgatg cgaagaagaa ctacgagtcg ttcgcgcagt tctccaagca cgacgccgac
     3121 gcgatcgagc gctgggacgc ctggatcggc ggcctcgccg acgtgctcgg ccccatgctg
     3181 atgtcgatcc cgcccaccgt tgggtcgaag aagcccgcgg acctctacga gcagctgcgg
     3241 ctggcgtggc ggttccgcgg gctcgatgtg aagacgatcg gggacgtcac ccgcctgatg
     3301 acgatgagca tcgtcgacat cctcgaccga ttcttcgaga ccgaccaggt gaagaccgtg
     3361 atggcgctca acggcctgat cggcacctgg gccggcccgt acgaaccggg caccggctac
     3421 gtgatggcgc accattcgat cggtgacgtc ggcgacggcc acctcggtgc gtgggccgtg
     3481 ccggaaggcg gcatgggcgc cgtcagcgac gcgatcgcgg ccagcgcccg cagcttcggc
     3541 gccgagatcc gcacggacgc ccgcgtcgcg aaggtgctca ccgagggtgg tcgggtgacc
     3601 ggcgtggtcc tggagggcgg cgaggagctc cgcgcgccgg tggtggtcac cgccatccat
     3661 ccgaagatca cgttcctgga gcagatcgac cggagcgagc tgcccgcggc gttcgtcgac
     3721 gacatcgaca actggaagac gcgcagcggc gtggtgaaga tcaactgtgc gctcgatcgc
     3781 gcgccggtgt tcaccgccaa gcccgatctg caggacctca ccggcgggtt cgagctcgcg
     3841 cactcgatca cctacctgga gaaggcgttc gaggaagcgc gcgccggcct gccctcgacc
     3901 gcgccgttca gcgacggcgt gatgcccacc tactacgacc gcacgctcgc acccgagggc
     3961 aagcacaccg tgtcgctgtt cacgcagtgg gtcccgcacg agtggtccac cgcgccgcac
     4021 cgcgacgatc tcgatgcata cgctgaccgg atcatcgacc aggtggaggc cgtcgctccc
     4081 gggttccgcg actcgatcct gcaccggcag gtgatcgggc cgtacgacat ggagcacacc
     4141 tacggcctgc tcggcggcaa catcttccac ggcgagctct
//
LOCUS       DATDBZ010000363         2479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_942164, whole genome shotgun sequence.
ACCESSION   DATDBZ010000363 DATDBZ010000000
VERSION     DATDBZ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942164"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /gene="truB"
                     /locus_tag="VIX62_08765"
     CDS             <1..807
                     /gene="truB"
                     /locus_tag="VIX62_08765"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY5860360.1"
                     /translation="AAVRRALGTKKVGHAGTLDPMATGLLVMGVGRATRLLRFLGDQA
                     KTYEGTFRLGVATTTLDADGDVIRTAPVEADDDQVRAAMTGQTGDLLQRPPAYSAVKV
                     GGRKLYEAARAGEDLRADARPVHVDTFEMTGRHSDDVDFLAVCGGGTYVRVLAADVGE
                     ELGCGAHLTRLRRTAIGGFHVEDARPPEEPGPPLSLEQAVAHLPRWNVEDEEAIAVSH
                     GRILAPSGLAGPFSVVAPDGRMLGIYEDAGTKAVPLVVLAPAGSNDPGTA"
     gene            complement(822..2441)
                     /locus_tag="VIX62_08770"
     CDS             complement(822..2441)
                     /locus_tag="VIX62_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5860361.1"
                     /translation="MQATAHRTRRPSGDPPPLPRDTHAMGWLWASLAVVIIGIGLAFI
                     LTNEPDLHEFGSNVLEWFADLRMSWLTQVAKVVVLISSFGAVQLLRLGIAGALVATKR
                     FRHLVVALVSFVLVDWLVLTFLSEQRPAPGVSVLADSGNYTFPSWYVTAFAITVFAMP
                     FVLAPAGRTRKRAMTAAWIAVILVCLARLYLGIDYPTNVAYAAVLCWVLSEALYRWFV
                     PDESFPVSYAKGGKAAHLDLSGARGEAVKTAMASQLGFDVAEVKPFGLAGSGGSSPLL
                     MTMADGTRVFGKIYATSHVRADRWYRVGRTIMYGKLEDETPFSSVRRLAEYEDYALRV
                     LAMNGVKVAQTWGIVELTPNREYMLVTGFFEDSKNLGDSEVDDTIIDEGLQLIRQFWD
                     IGVAHRDVKPANLLVQRGDLQLVDVSGLECRPTPWRQAVDLANMMLTLALQSDPDRVY
                     QRATAFFTPDEIAEAFACAEGMAIPTELSGRLKQDPRPIMQRFKELAPAHAPVSIQRW
                     SFQRIALTAAVVLGSLILLTMAIDSLFAGLK"
BASE COUNT          402 a          847 c          872 g          358 t
ORIGIN      
        1 gcggccgtgc gacgagcgtt gggcacgaag aaggtcggac acgcgggcac cctcgatccg
       61 atggcgaccg gtctgctggt catgggcgtc ggccgggcca cccggctcct gcggttcctc
      121 ggcgatcagg ccaagaccta cgagggcacg ttccgcctgg gggtcgcgac caccaccctg
      181 gacgccgacg gtgacgtcat ccgcacggcg ccggtggagg ccgacgatga ccaggttcgg
      241 gcggcgatga ctgggcagac gggcgatctg ctccagcgcc cgcccgccta ctcggccgtc
      301 aaggtcggtg gcaggaagct gtacgaagcg gcccgggcgg gcgaggacct ccgagccgac
      361 gctcggccgg tccacgtcga caccttcgag atgacgggac gccacagcga cgacgtcgac
      421 ttcctcgcgg tctgcggcgg cggcacctac gtccgggtcc tggcggccga cgtgggcgag
      481 gagctcggat gcggggctca cctcacgagg cttcgacgca ccgcgatcgg tgggttccac
      541 gtcgaggacg cgaggccacc cgaggaaccc ggcccgccgc tgtcgttgga gcaggcggtg
      601 gcgcacctcc cgcgttggaa cgtcgaggac gaagaggcga tcgcggtctc ccacggcagg
      661 atcctggcgc cgtccggcct cgccggtccg ttctcggtcg tcgcacccga cggtcgcatg
      721 ctgggcatct acgaggacgc cggcacgaag gcggtcccct tggtggtcct ggcccccgct
      781 gggtcgaacg accccgggac cgcctgagtc gtcggtcgcg cctacttcag cccggcgaac
      841 agcgagtcga tcgccatcgt cagcaggatg agcgatccca gcacgaccgc ggcggtgagc
      901 gcgatccgct ggaagctcca ccgctggatc gagaccgggg cgtgggcggg ggcgagttcc
      961 ttgaagcgtt gcatgatcgg gcgtgggtcc tgcttcagcc ggcctgagag ttcggtgggg
     1021 atcgccatgc cctcggcgca ggcgaacgct tcggcgatct cgtcgggggt gaagaacgcc
     1081 gtcgcccgct gatagacccg atctgggtcg gactgcaacg ccagggtcag catcatgttc
     1141 gccaggtcga cggcttggcg ccacggcgtg ggccggcact cgaggcccga cacgtcgacc
     1201 agctggaggt caccacgctg caccagcagg ttcgcgggct tcacgtcgcg gtgcgcgacg
     1261 ccgatgtccc agaactggcg gatcaactgg aggccttcgt cgatgatcgt gtcgtccacc
     1321 tcggagtccc cgaggttctt cgaatcctcg aagaagccgg tcacgagcat gtactcccgg
     1381 ttgggcgtga gctccacgat gccccaggtc tgcgccacct tcacgccgtt catcgccagc
     1441 acgcgcagcg cgtagtcctc gtactcggcg agccggcgga ccgacgagaa cggcgtctcg
     1501 tcttcgagct tgccgtacat gatcgtgcgt cccacccggt accagcggtc ggcgcgcacg
     1561 tggctcgtgg cgtagatctt gccgaacacg cgggtgccgt cggccatcgt catgagcagc
     1621 ggcgaggagc ctccggagcc agcgagcccg aacggcttga cctcggcgac gtcgaacccg
     1681 agctggctcg ccatcgccgt cttgaccgct tcgccacggg cgccgctcag gtccaggtgg
     1741 gccgccttgc cgcctttcgc gtaactcacc gggaacgatt catcgggcac gaaccagcgg
     1801 tagagggcct ccgacagcac ccagcagagc acggcggcgt acgcgacgtt cgttgggtag
     1861 tcgatgccca ggtagaggcg ggcgaggcac accaggatca ccgcgatcca cgccgccgtc
     1921 atcgctcgct tccgggtccg gccggcgggc gctagcacga acggcatcgc gaacaccgtg
     1981 atcgcgaacg ccgtgacgta ccaggacggg aacgtgtagt tgccgctgtc cgccaatacg
     2041 ctcacaccgg gagccggacg ctgctcggaa aggaacgtca gcacgagcca gtccaccagc
     2101 acgaacgaca ccagggcgac gacgaggtgg cggaaccgct tcgtcgcgac cagcgccccg
     2161 gcgatcccga gccgcaacag ctgcacggcg ccgaacgagg agatcaggac cacgaccttg
     2221 gcgacctgcg tgagccagga catgcgtagg tccgcgaacc attcgaggac gttgctgccg
     2281 aattcgtgca gatccggctc gttcgtcagg atgaaggcga gcccgatccc gatgatcacg
     2341 acggccagcg acgcccagag ccagcccatg gcatgggtgt cgcgcggcag cggcggcggg
     2401 tcgcccgacg gccggcgggt tcggtgcgcg gtggcctgca tccggggcag cctatccggt
     2461 gcacagggtc cgagcgacc
//
LOCUS       DATDBZ010000364         2100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_332866, whole genome shotgun sequence.
ACCESSION   DATDBZ010000364 DATDBZ010000000
VERSION     DATDBZ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332866"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1364
                     /locus_tag="VIX62_08775"
     CDS             192..1364
                     /locus_tag="VIX62_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY5860362.1"
                     /translation="MTRAGGWRVPMAGRDREESHRASTPLELFFDLVIVIAVAAASRS
                     LHHGIAEDHVPESILSYLMVFFAIWWAWMSFSWFATAYDTDDVPYRVGVLVQMSGAII
                     IAAGVGRAFEAGDWTVVTLGYVVMRLATVALWMRAARGDPEHRANSMRWVMGVTIAQV
                     GWILLLFAPDGLKLPGFLFLVVAELFVPIWAARAKRMRFHAEHIAERYGLFTIIVLGE
                     SVLAGTVAIGTAAEEVAGIPDVAILGVALGALLIVFVMWWLYFERSSNDLLTTIPRSF
                     EWGYGHYVIWAAAAAVGAGVAVAVDVATHHAEVSTTVAGAAVAVPVAVYVMGTWLLHD
                     VPRPMPAWRMALSPIAALLVLMAPLTATPVLAVGVIVVALLVAKVATADVQPSPHA"
     gene            complement(1369..1665)
                     /locus_tag="VIX62_08780"
     CDS             complement(1369..1665)
                     /locus_tag="VIX62_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860363.1"
                     /translation="MAAINVIRQRVPMLDAPTLEDRTRPALGDVGRVPIRQRFAERTM
                     SSYLGWGIADQRVGGLSRADRTPSQRQLTQDQRRRTPGQLPRMAVVVRPRSTVG"
BASE COUNT          273 a          725 c          741 g          361 t
ORIGIN      
        1 cggcgtgagg gcctgtcggg tgggctcgtc ggtgtcgttc gcgcgccaac cgttcggcgc
       61 cagcggacga cagcgaggtg agggcctgtc gggtggtctc gtcggtgtcc ttcgggcgtg
      121 tttcgttccg tggcagagga cgccgaaccc ttccgtggcg gacgctcgac cgactgctac
      181 cgttcgcggc catgacgaga gcaggcggct ggcgcgtgcc catggctgga cgggaccgcg
      241 aggagtcgca tcgggcgtcc acgccgctcg agctcttttt cgacctcgtc atcgtcatcg
      301 ccgtggcggc cgcatcgcgg tcactccacc acggcatcgc cgaggaccac gtccccgagt
      361 ccatcctcag ctacctgatg gtcttcttcg ccatctggtg ggcgtggatg agcttctcct
      421 ggttcgccac cgcctacgac acggacgacg tgccctaccg cgtgggcgtc ctcgtgcaga
      481 tgagcggcgc gatcatcatc gcggccgggg tcgggcgagc cttcgaggct ggcgactgga
      541 ccgtggtgac cctcggctac gtcgtcatgc gcctggccac ggtggcactg tggatgcgag
      601 ccgcacgcgg cgaccctgag catcgggcca actcgatgcg ttgggtcatg ggcgtgacca
      661 tcgcgcaagt cggctggatc ctgctgctct tcgcaccaga cggcctgaag ctaccgggct
      721 tcctgttcct cgtcgtggcg gagctgttcg tgcccatctg ggcggcccgc gccaagcgca
      781 tgcgcttcca cgccgagcac atcgccgagc gctacggcct gttcacgatc atcgtccttg
      841 gcgagagcgt gttggctggc accgtcgcca tcggcaccgc cgccgaggag gttgcgggca
      901 tcccggacgt cgccatcctc ggcgtggcgc tcggggcgct tctcatcgtc ttcgtcatgt
      961 ggtggctcta cttcgagcgc agcagcaacg acctgctcac cacgatcccg cgctcattcg
     1021 agtggggcta cggccactac gtcatctggg ccgccgcagc agccgtcggc gccggcgtgg
     1081 ccgtggccgt cgatgtggcg acccaccacg ctgaggtcag tacgaccgtg gctggtgctg
     1141 ccgtggccgt gcccgtggcg gtctacgtga tggggacgtg gctcctgcac gacgtgcctc
     1201 gtccgatgcc cgcctggcgg atggccctca gcccgatcgc cgcactcctc gtgctcatgg
     1261 cgccgctgac ggcgacgccg gtgctcgccg tcggggtcat cgtcgtggcg ctgctcgtgg
     1321 cgaaggtcgc cacggccgac gtgcagccct cgccgcacgc ctgaacggtc agccgaccgt
     1381 cgatcggggt cggacgacga cagccatcct ggggagctgg ccaggcgtcc ggcggcgttg
     1441 gtcctgcgtc agttggcgct gcgaaggcgt gcggtcggcg cgcgagagtc ccccgacgcg
     1501 ttggtcggcg atcccccacc ccagatatga cgacatcgtc cgttccgcga agcgctgccg
     1561 gatcggcacc cggccgacat cgcccagcgc ggggcgggtg cggtcctcca gggtcggcgc
     1621 gtcgagcatc ggcacacgtt ggcggatgac gttgatggcg gccacgtaca gtccctccct
     1681 gctggcgacc ctcgtagatc cggcctgggc aaagccggat cggcacaggg cgccgcccat
     1741 tgacgtggca cctgcccgga gtctaccgag gtttcgtgcg catgggcaag ttccggcgga
     1801 ctggagatcg acgggttcgt cggatgcgaa cgccccaggg gcgtcgccgg ccacgggggt
     1861 tgcgtgccag tagcacgacc gacgtggtca cacccgtcgc gtgcctctgg tgtgcggcat
     1921 agtcgcatgg gagcggtctc cgtgtcggcc acgtgcgccc cagtggcacg gtagtcgaac
     1981 aggggccagg acaggtcaca cgtgccactg ggccgcacgc gcgaacaggg aggctgccac
     2041 gcgggccctg ccgcgcgaac cctgccgcgc gagcccttaa ccgcgcgagc tcgcacggtt
//
LOCUS       DATDBZ010000365         3144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_943042, whole genome shotgun sequence.
ACCESSION   DATDBZ010000365 DATDBZ010000000
VERSION     DATDBZ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943042"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VIX62_08785"
     CDS             <1..432
                     /locus_tag="VIX62_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5860364.1"
                     /translation="VGVPGAWAAGDIAAVPDRTTGGTSPPTAQHAMRQGKRLGDNLTA
                     SELGGALQPFVWKNVGGVCSLGRYKGVANVFGVKVKGFAGWFLHRSYHLYAMPTIGRR
                     VRIAVDWTIALLFPRDITQFSGFEDPRGPFRRAAGSGPDAS"
     gene            535..2226
                     /gene="sulP"
                     /locus_tag="VIX62_08790"
     CDS             535..2226
                     /gene="sulP"
                     /locus_tag="VIX62_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEY5860365.1"
                     /translation="MLAGYRREWLTKDIVAGLVLTALLVPQGMAYAELAGLPAITGLY
                     TSIMCLVAYAMFGPSKILVLGPDSSLGPMIAATLAPLLAADGDPAKAVAMASVLALMV
                     GGVMVVAGTAKLGFIADLLSKPTVMGYMNGLAVTILVGQLPKLFGFSVDADGFIDELT
                     GFLRGVANGETVTAALAIGALGLVLMVVLDRVLPKVPGVLVVVIISIALAAIFDLESR
                     GVSLVGALPQGFPPFTIPTVSLGDLGALALGALGIALVSLTDTISTATSFAARTGQEI
                     DGDHELIGIGAANLAAGLFQGFPVSTSGSRTAVAEQAGAKTQVAGLVGAGAITLMLLF
                     VPGLLRNLPQPTLAAVVIVASVTLADIPGTIRLWRQRHTEFYLAMAAFLGVVLLGVLP
                     GIGLAIALSILSVFRRSWWPYQAELGKVEGLEGFHDVSSYPEAELLPGCVLFRFDAPL
                     FFANSRAFRDQIRRLARSDPPPAWIVVAAEPITDVDTTAADMLEDLDEALNEAGIHLV
                     FAEMKDPVRRKVERYELTRTIAPEHFYPTIDAAVEAFRAMTGAEWQPAERGDDER"
     gene            2216..>3144
                     /locus_tag="VIX62_08795"
     CDS             2216..>3144
                     /locus_tag="VIX62_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY5860366.1"
                     /translation="MSASSSGSSTGWRRIAAIVSIVTLIAALGLVVIATAASLGGVLM
                     VLVGVLIVVGGGWHAVSRRGLVRTISVVVAILGVVLIVTGLVVGDFSIERGFTIVVLS
                     AISVGTARVALHRTDKDLAATVSSLPSAPPAGRPVLIMNPRSGGGKVGKFHLEDECRS
                     RGIEPVVLSPGDDLLALAEDAIARGADVIGMAGGDGSQALVASVASKHDVPHVVIPAG
                     TRNHFALDLGMDRDDVVGALDAYTDGVERRIDLASVNGTTFVNNASMGLYAKIVQSPE
                     YRDAKVQTVLDMLPDLLGPDAVPLDLRYVGPDG"
BASE COUNT          431 a         1028 c         1143 g          542 t
ORIGIN      
        1 gtgggcgtgc cgggcgcctg ggccgccggg gacatcgcgg ccgttcccga tcgcacgacc
       61 ggtggcacga gccctcctac ggcccaacac gcgatgcgcc aaggcaaacg gctcggggac
      121 aacctcaccg cgtccgaact cggcggagcg ctccagccgt tcgtatggaa gaacgtcggt
      181 ggcgtgtgtt cgctgggccg gtacaagggc gtcgccaacg tgttcggcgt gaaggtgaag
      241 ggattcgcgg gctggttcct gcaccgctcc taccacctgt acgccatgcc cacgatcggc
      301 cggcgtgtgc ggatcgcggt cgactggacg atcgcgctgt tgttcccccg agacatcacg
      361 cagttcagcg gattcgagga tccgcgggga ccgttccggc gagccgccgg ctccggcccc
      421 gatgcgagct gacccaggcg atggcgctgc gcgcgccaag tccctaggct tcccacgacg
      481 acgacagctg gggggagggg atcagtgaag gcgaccgcgg tgccgggcgt ccgcatgctg
      541 gccggctacc gtcgcgagtg gctcaccaag gacatcgttg ccgggctggt cctcaccgca
      601 ttgctcgtgc cccagggcat ggcgtacgcg gaactcgcgg gcctgccggc gatcaccggc
      661 ctctacacct cgatcatgtg cctggtcgcc tacgcgatgt tcggaccgag caagatcctg
      721 gtgctcggcc ccgactcctc actgggtccc atgatcgcgg cgacgctggc gccgctgctg
      781 gccgcggacg gggacccggc gaaggcggtg gcgatggcct ccgtgttggc gctgatggtg
      841 ggcggcgtca tggtcgtcgc gggcacggcg aagctcgggt tcattgccga cctgctgagc
      901 aagcccacgg tgatgggcta catgaacggg ctcgcggtga cgatcctggt gggccagttg
      961 ccgaagctgt tcgggttctc ggtcgacgcc gacgggttca tcgacgagct caccgggttc
     1021 ctccgagggg tggccaacgg cgagacggtg acggctgcgt tggcgatcgg tgcgctgggg
     1081 ctggtgctga tggtggtcct cgaccgcgtg ctccccaagg ttcctggcgt actcgtggtc
     1141 gtcatcatct cgatcgcgct ggcggcgatc ttcgacctgg agtcccgggg cgtgtcgctg
     1201 gtcggtgcgt tgccgcaggg gttcccgccg ttcacgatcc cgacggtttc gctcggcgac
     1261 ctgggagcgc tcgcgctcgg cgcgctcggt atcgcgctgg tgtcgctgac ggacacgatc
     1321 tcgacggcga cctcgttcgc cgcccgcacc ggccaggaga tcgacggcga ccacgaactg
     1381 atcgggatcg gcgcggcgaa cctcgcggcc ggtctgttcc agggattccc ggtgagcacc
     1441 agcggctcgc ggacggcggt cgccgagcag gcaggggcga agacgcaggt ggccggactg
     1501 gtgggcgccg gagccatcac gctgatgttg ctgttcgtgc cgggcctgtt gcggaacctt
     1561 ccccagccga ccctggccgc cgtggtgatc gtggcttcgg tcaccctcgc cgacatcccg
     1621 ggcacgatcc ggctgtggcg gcaacgtcat accgagttct atctggcgat ggcggcgttc
     1681 ttgggcgtcg tcctgttggg ggtcctgccc ggtatcgggc tcgccatcgc gttgtcgatc
     1741 ctcagcgtgt tccggcgttc gtggtggccg tatcaggcgg agctcggcaa ggtcgagggg
     1801 ctggaaggct tccacgacgt ctcgagctat cccgaggccg agctcctgcc ggggtgcgtg
     1861 ctgttccggt tcgacgcgcc gctgttcttc gccaactcgc gtgccttccg cgatcagatc
     1921 cggcggctcg cccgctccga tccgccgcct gcatggatcg tggtggccgc cgaaccgatc
     1981 accgacgtcg acacgaccgc cgccgatatg ctcgaggatc tcgatgaggc cctgaacgag
     2041 gcggggatcc acctggtgtt cgccgagatg aaggacccgg tcaggcgcaa ggtggagcgg
     2101 tacgagttga cgaggacgat cgcgccggag cacttctacc cgacgatcga cgccgccgtc
     2161 gaagcgttcc gagcgatgac cggcgccgag tggcagcccg cggaacgggg cgacgatgag
     2221 cgctagctcc tccggttcct cgacggggtg gcgacggatc gcggccatcg tgtcgatcgt
     2281 gacgctgatc gcggcgttgg gactggtggt catcgcaacg gctgcgagtc tcgggggcgt
     2341 gctgatggtg ctcgtcggcg tgctcatcgt cgtcggcggg ggatggcatg cggtgtcacg
     2401 acgcggcctg gtgcgcacga tctcggtggt agtggccatc ctgggcgtcg tgctcatcgt
     2461 gaccggcctg gtggtcgggg acttctcgat cgagcgcggc ttcacgatcg tggtgctgtc
     2521 ggcgatctcg gtggggacgg ccagggtcgc actccatcgc acggacaagg atctggccgc
     2581 caccgtgtcc tcgctgccct cggcgccgcc cgccggccga cccgtgctga tcatgaaccc
     2641 ccggtccggc gggggaaagg tcgggaagtt ccacctggag gacgagtgcc gatcacgggg
     2701 gatcgagccc gtcgtcctgt cgccgggcga cgacctcctg gccctggccg aggacgccat
     2761 cgctcgcggc gccgacgtga tcggcatggc gggcggcgac ggctcccagg cgctcgtcgc
     2821 ctcggtggcg agcaagcatg atgttcccca cgtggtgatc cccgccggca cgaggaacca
     2881 tttcgcgctg gacctgggga tggaccgtga cgacgtcgtg ggagcccttg acgcctacac
     2941 cgacggggtc gagcggcgga tcgacctggc gtcggtcaac ggcaccacgt tcgtcaacaa
     3001 cgcatcgatg gggctgtacg cgaagatcgt gcagtcgccc gaataccgcg acgcgaaggt
     3061 gcaaaccgtg ctcgacatgc tgcccgacct gctcggaccg gacgccgtgc cgctcgatct
     3121 gcgctacgtc gggcccgacg ggga
//
LOCUS       DATDBZ010000366         4515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_333294, whole genome shotgun sequence.
ACCESSION   DATDBZ010000366 DATDBZ010000000
VERSION     DATDBZ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333294"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VIX62_08800"
     CDS             <1..637
                     /locus_tag="VIX62_08800"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY5860367.1"
                     /translation="LEATSEATWGVFCEATEVWGVPLGQLSDNGLNFSGKLRGFEVFF
                     ERQLRAVGVRPTTSRPYHPQTCGKVERFQQTLKKWLRHQPVAADLAELQAQLDAFVHY
                     YNHHRPHRAIGRITPVERWAATPPAINLGIALPSPTQATTVVVADNGVVGVGRYTIAI
                     GREWRRRHARVHHDNTHAAIFIDHQLIRAVTLDPTRRYQPSGLSQGRRPKP"
     gene            complement(805..1995)
                     /locus_tag="VIX62_08805"
     CDS             complement(805..1995)
                     /locus_tag="VIX62_08805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5860368.1"
                     /translation="MRDRRLVLTFGLLTALFAAGYGVMFTMLDDFRDEFGIGPTALGA
                     IVATGFFSSFLAQVLLAPLADRGHARRLVYLGMLLNVVGLVGMAVGTTFLTLVLARLV
                     MGIGAGMAQPAIRRIVILSAPDELGANLGFLLSAEVGGFAMGPAVAAILTGPFGIPAP
                     FLLIAVATVLCLPVIARADVQETVDDAAPAARFAFDLLRSRPYVAAVMMGSAVFLMIG
                     TFDALWVLVLSDLETADWIANLGITLFALPLVVLGSYGGRLAQRVGPYRLGTLGLLGG
                     ATFMCLYGVLPTGGAMFAVAMVHALNDGLTVSSASVAVGLVSPADRQAGAQGLLGGIE
                     TLVAGVTALAAGALYEEFDRTVAYTACAVAMVTCVLVAYALVGSEWRRRPARIEMVSA
                     GSRR"
     gene            complement(2116..3078)
                     /locus_tag="VIX62_08810"
     CDS             complement(2116..3078)
                     /locus_tag="VIX62_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5860369.1"
                     /translation="MEYRRLGRSGLSVSALSFGSWVTFGNQLDTSLARDCLTAAREAG
                     VNFFDNAEVYAGGESERIMGAAIAELGWPRHEYVISTKFFWGIRDGLVNMSNTLNRKY
                     LMQAIDGSLERMGLDFVDLAYCHRADPKTPIEETVWAMSDMVSSGKALYWGTSEWSAD
                     EIRAAWLIADRHHLHKPVVEQPQYNMFERRKVEQEYARLYEDLGIGLTTWSPLASGLL
                     SGKYIDGIPEGSRASLPGYEWLRDELTNPKRNERVRKLAAIAERLDCSLSQLAIAWCA
                     SNPNVSTVITGASRVEQVHENMGAMAVVALLTPDVLDEIRGTIR"
     gene            3208..4017
                     /locus_tag="VIX62_08815"
     CDS             3208..4017
                     /locus_tag="VIX62_08815"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY5860370.1"
                     /translation="MRHWRRRRAAGTIDDAAAADASIRVVVARRLPAWQHLDDPERDR
                     LVDITIELVREMRWEAARGFELTDEMVLTIAAHAALPVLELGAERYREVSTIIVHPRT
                     IVLTGAQPGPVPGTMSDDPEHLLGQAHYRGPVLLAWAAVLRQAMRPARGEDVVIHEFA
                     HRLDMLDGLVDGTPPLGDSDAHERWVAVCTAEFERLRTGEADPLLRRYAAVDPGEFFA
                     VVSETFFTRPVELEAAKPDLYDVLRGFYRQDPAARERRTAERIEADQPLDR"
     gene            complement(4028..>4515)
                     /locus_tag="VIX62_08820"
     CDS             complement(4028..>4515)
                     /locus_tag="VIX62_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5860371.1"
                     /translation="RSRGGREMKVSDVVLRSPVTVPGDATIGDAARAMARDGVGSVIV
                     VEGDRPIGVLTDRDLVVRGIARGLPADARADGLMSIGVVAIDHDADVREAVRIFGTHP
                     FRRLPVVDGHRVVGVVSVDDLLIALTSELSDVTRGLSAQVMFPHGGDEPRLPVATEVM
                     P"
BASE COUNT          797 a         1631 c         1463 g          624 t
ORIGIN      
        1 gctcgaggcc accagtgagg ccacctgggg cgtgttctgc gaggccaccg aggtgtgggg
       61 cgtgcccctc ggtcagctca gcgacaacgg cctcaacttc tccgggaagc tacgcgggtt
      121 cgaggtgttc ttcgagcgac agctccgcgc cgtcggagtc cggcccacaa cctcccggcc
      181 ttaccacccc cagacctgcg gcaaagtgga acgcttccaa cagaccctca agaaatggtt
      241 gcgtcaccag ccggtagccg ccgacctggc cgagctacaa gctcaactcg acgccttcgt
      301 ccactactac aaccaccacc gcccccaccg cgccatcggg cgcatcaccc cagtcgagcg
      361 gtgggcggcc acgccgccgg cgatcaacct cggcatcgcc ctgcccagcc ccacacaggc
      421 cacgaccgtc gtggtggccg acaacggagt cgtgggcgtc ggccgctaca ccatcgccat
      481 cggcagggaa tggcgccgtc gccacgcccg cgtccaccac gacaacaccc acgcagcgat
      541 cttcatcgac caccagctca tccgcgccgt cacgctcgac cccacccggc gctaccaacc
      601 ctccgggcta tcacagggcc gccgacccaa gccctaacat caaccgtcag ggatgtcccg
      661 cgacacatct gtcagacctg tcgcgcgaca caacagttgc gctatccgca accgagcgca
      721 caaacgcgag cgtcacgccg ccaggcgcag cgagccgatc gggagcagcc gaagcgaagc
      781 ggcggccagt cccgatccat cagatcagcg gcgggagccg gctgacacca tctcgatcct
      841 ggccgggcgg cgccgccatt ccgaaccgac cagcgcatag gcgacgagga cgcacgtcac
      901 catggcgacc gcacatgcgg tgtaggcgac ggtgcgatcg aactcctcgt acagcgcacc
      961 ggccgcgagc gcggtcaccc cggcgacgag cgtctcgatg ccaccgagca agccttgcgc
     1021 gcccgcctgg cgatcggccg gcgacacgag accgacggca acgctcgcgc tcgacacggt
     1081 cagcccgtcg ttgagcgcat gcaccatcgc cactgcgaac atcgcgccgc ccgtcggcag
     1141 tacgccgtac aggcacatga aggtggcgcc acccaacaag ccgagcgttc ccagccggta
     1201 cggccctacc cgctgcgcca ggcggccgcc gtacgaaccg agcacgacca aggggagcgc
     1261 gaacagcgtg ataccgagat tggcgatcca gtcggccgtc tccagatcgc tcaacaccag
     1321 cacccagagc gcgtcgaagg tgccgatcat caagaagacg gcagagccca tcatcaccgc
     1381 ggccacgtac ggccgagacc gcaacaggtc gaacgcgaac cgggctgccg gcgcggcgtc
     1441 gtcaacggtc tcctgcacgt ccgcgcgagc gatcacgggc aggcacagga cggtcgcgac
     1501 ggcgatcagg aggaacggcg ccgggatgcc gaacgggccg gtgaggatgg cggccacggc
     1561 cgggcccatc gcgaacccgc cgacctcggc cgacagcaga aagccgaggt tcgcgccgag
     1621 ctcgtccggc gcagagagga tcacgatgcg gcggatggcg ggctgggcca tccccgcgcc
     1681 gatgcccatc accagccggg cgaggacgag cgtcaggaac gtggttccca ccgccatgcc
     1741 gacgaggccc accacgttga gcaacatgcc gagatagacc agccggcggg catgccctcg
     1801 atcagcgagt ggcgccagca ggacctgggc gaggaacgac gagaagaatc cggtggcgac
     1861 gatcgcgccg agcgcagtcg ggccgatgcc gaattcgtcg cgaaagtcgt cgagcatcgt
     1921 gaacatgacg ccgtagccgg cggcgaacag cgcggtcagg agaccgaacg tgaggacgag
     1981 ccggcgatcg cgcatgggcc acgaccgtac caacgctcct gaagtgaacc tcttccgatt
     2041 ctcccgccgc gcctcccgcc gcgcctcccg ccgcgcctcc cgctgcgcca gccgccgaga
     2101 cggtgcagaa cggcgtcagc ggatcgtgcc gcggatttcg tcgaggacgt cgggggtgag
     2161 cagagcgacg accgccatgg cacccatgtt ctcgtggacc tgctcgacgc ggctcgcacc
     2221 cgtgatgacc gtcgagacgt tcgggttcga cgcgcaccac gcgatcgcga gctgcgacag
     2281 ggagcagtcc agacgctcgg cgatggcggc cagcttacgg acgcgctcgt tgcgcttcgg
     2341 gttcgtcagc tcgtcacgca gccactcgta cccgggcagc gaggcccggc tgccttccgg
     2401 gatcccgtcg atgtacttgc ccgagagcag gccggaggcg agcgggctcc acgtggtcag
     2461 cccgatgccg aggtcctcgt acagccgggc gtactcctgc tcgaccttgc gccgctcgaa
     2521 catgttgtac tgcggctgct cgaccacggg cttgtggagg tggtgacgat cggcgatcaa
     2581 ccacgcggcg cggatctcat cagcgctcca ctcggacgtg ccccagtaga gcgccttgcc
     2641 cgaggacacc atgtcggaca tcgcccatac ggtctcttcg atcggggtct tcgggtcggc
     2701 ccggtggcag tacgcgagat cgacgaagtc gagccccatg cgttccagcg aaccgtcgat
     2761 ggcctgcatc aggtacttcc ggttgagggt gttcgacatg ttgacgagtc catcgcggat
     2821 gccccagaag aacttcgtcg agatgacgta ctcgtgccgg ggccagccca gttcggcgat
     2881 ggcggcaccc atgatccgct ccgattcgcc tccggcgtac acctcggcgt tgtcgaagaa
     2941 gttgaccccg gcctcccggg cggcggtcaa gcagtcgcga gcgaggctgg tgtcgagttg
     3001 gttcccgaac gtgacccacg acccgaagga gagcgcgctc acgctcaggc cggagcgccc
     3061 gaggcggcgg tactccatcg ttgtggtcgt gccatctgcc atagccgtca tctcctgggt
     3121 tcgacgagag ttctccggcg gcaaaggtac ccacgccccc accgtcgata acgtccgacc
     3181 ggtgatcagt cgggccgagg tcgagtggtg aggcactggc gacgacgccg cgccgcggga
     3241 acgatcgatg acgccgcagc cgccgacgca tcgatccggg tcgtcgtggc ccggcgcctc
     3301 ccggcctggc agcacctcga cgaccccgag cgggaccgcc tcgtcgacat caccatcgag
     3361 ctggttcggg agatgcggtg ggaagctgca cgcggtttcg agctcaccga cgagatggtg
     3421 ctgacgatcg ccgctcacgc cgcactgccc gtgctcgagc tcggagccga gcggtaccgc
     3481 gaggtctcta cgatcatcgt gcacccgcgc acgatcgtgc tcaccggcgc ccagcccggc
     3541 ccggtgccgg gcaccatgtc ggacgatccc gagcacctgc tcggccaggc ccactatcgc
     3601 ggtcccgtgc tcctggcgtg ggctgccgtg ctccgtcagg cgatgcgtcc ggcacgcggg
     3661 gaggacgtgg tgatccacga gttcgcccat cgtctcgaca tgctcgacgg gctggtcgac
     3721 ggcacgccgc cgctcgggga cagcgacgcc cacgagcgtt gggtcgctgt ttgcacggcc
     3781 gagttcgagc gcctccgcac cggggaagcc gacccgctgc tacggcggta tgccgccgtc
     3841 gatcccgggg agttcttcgc cgtggtcagc gagacgttct tcacgcgacc ggtcgaactg
     3901 gaggcggcga agcccgatct ctacgacgtg ctgcggggct tctaccgtca ggatccggcc
     3961 gcccgcgagc gtcgcactgc cgaacggatc gaggcggacc agccactcga tcgttgaccc
     4021 gctcgggtca gggcatgacc tccgtcgcta ccggcaaccg cggctcgtca cctccgtggg
     4081 ggaacatcac ctgcgcactc aggccacgcg tcacgtcgct gagctcgctc gtgagcgcga
     4141 tgagcaggtc gtccaccgac acgacgccga cgactcgatg accgtcgacg acgggaaggc
     4201 ggcggaacgg gtgcgtaccg aagatgcgca ccgcctcccg gacatcggcg tcatggtcga
     4261 tggcgacgac gcctatgctc atcagcccgt cggctcgggc gtcggccggc agacctcgcg
     4321 caatgccccg cacgacgagg tcgcgatcgg tgagcacgcc gatcggtcgg tcgccctcga
     4381 cgacgatcac ggagccgacg ccgtccctcg ccatggcgcg agcggcatcg ccgatcgtgg
     4441 catcaccggg aaccgtcacc ggagaacgca acaccacatc actgaccttc atctccctac
     4501 ctcctcgtga acgcc
//
LOCUS       DATDBZ010000367         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1128346, whole genome shotgun sequence.
ACCESSION   DATDBZ010000367 DATDBZ010000000
VERSION     DATDBZ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128346"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VIX62_08825"
     CDS             <1..371
                     /locus_tag="VIX62_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012185001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEY5860372.1"
                     /translation="VKQLLDEVHEAPLDEASRERLAEIYERSVTELSMALSPDLQEEL
                     HILALPFDQESVPTEAELRIAKAQLVGWLEGLFHGIQATLMAQQFAARQQLEQMRELP
                     KGPGGVPPMGPGVERPGTYL"
     gene            506..>2083
                     /gene="dnaE"
                     /locus_tag="VIX62_08830"
     CDS             506..>2083
                     /gene="dnaE"
                     /locus_tag="VIX62_08830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY5860373.1"
                     /translation="MPDSFTHLHVHTEFSMLDGAARLDELVAKAVGDGQPAIGITDHG
                     NMYGTLDFYRECNKQGIKPIIGTEAYMAHDHRSERPTRRGRIDDSGGDTEGGTKLYYH
                     LTLLAENQTGYKNLIQLSSLAFMEGYYYKPRMDWELLEKYSEGLIATTGCLGGHVLQS
                     LLNGDEKGALAKAARLQDIFGRDNLFVELQDHGLQAQRDTNPKLIEIAKQIGAPLLAT
                     NDSHYTHQHDHESHDALLCVQTGALLSDPKRFKFEGTEHFLKTAAEMRYLFREVPTAC
                     DNSLWIAERAELEIEFGKPLLPNFPLPAGFADDAAYLTDLTWRGAAERWGDELPPDVA
                     ERLGYELKVICDMGFASYFLITWDLIKYAKDRGIRVGPGRGSAAGCAVAYALRIPDLD
                     PVRYDLLFERFLNPSRVSMPDIDMDFDSRYRDEMIRYAAERYGRDHVAQIITFGTIKA
                     RNAVRDAARVLGYPYGIGDKLAKAMPPMVMGRDTPLKYCFEENPKYADGYKAAADLRA
                     MYDTDPDLKRVIDVARGL"
BASE COUNT          390 a          741 c          650 g          302 t
ORIGIN      
        1 tggtcaagca gctcctcgac gaggtgcacg aggcaccgct cgacgaggcc tcccgcgaac
       61 ggctcgccga gatctacgaa cgttcggtca ccgaactgtc gatggcgctg tcgccggatc
      121 tgcaggagga gctgcacatc ctcgccctgc ccttcgatca ggagagcgtg ccgaccgagg
      181 cggagctccg gatcgcaaag gcccagctgg tcgggtggct cgaggggctg ttccacggga
      241 tccaggccac gttgatggcc cagcagttcg ccgcccgcca gcaactggaa cagatgcgcg
      301 agctgcccaa gggtccgggc ggggtgcccc cgatgggccc cggcgtcgaa cgccctggca
      361 cctacctgtg accacgcact cgggtgcgat gcgaccgctg aacgcgcgcg acgctttcgg
      421 ctacattcga accacaacgt tgtaacttcc gaggtccgcc cgcatcggcg aaccgtcacc
      481 agccacccgc caccgtcagc agtccgtgcc cgattccttc acccacctcc acgtccacac
      541 cgagttctcg atgctcgacg gcgcggctcg gctcgacgaa ctcgtcgcca aggcggtcgg
      601 cgacggccag ccggcgatcg gcatcaccga tcacggcaac atgtacggaa cgctcgactt
      661 ctaccgcgag tgcaacaagc aggggatcaa gccgatcatc ggcaccgagg cctacatggc
      721 ccacgaccac cgcagcgagc gaccgacccg ccgaggccgg atcgacgact cgggcggcga
      781 caccgagggc ggcacgaagc tctactacca cctgacgctg ctggccgaga accagaccgg
      841 ttacaagaac ctgatccagc tctccagcct ggccttcatg gagggctact actacaagcc
      901 ccggatggac tgggagctgc tcgagaagta ctccgaaggc ctcatcgcca ccaccggctg
      961 cctcggcggc cacgtcctgc aatcgctcct gaacggcgac gagaagggtg cgctcgccaa
     1021 ggcggcccgg ctgcaggaca tcttcgggcg cgacaacctg ttcgtcgaac tccaggacca
     1081 cggtctgcag gcgcagcgcg acacgaaccc gaaactgatc gagatcgcca agcagatcgg
     1141 cgccccgctc ctcgccacca acgacagcca ctacacgcac cagcacgacc acgagtccca
     1201 cgacgcgctc ctgtgcgtcc agacgggggc cctgttgtcg gacccgaagc gcttcaagtt
     1261 cgaagggacc gaacacttcc tgaagacggc cgccgagatg cgctacctgt tccgggaggt
     1321 cccgacggcg tgcgacaact cgctgtggat cgccgaacgc gccgaactcg agatcgagtt
     1381 cggcaagccg ctgctgccga acttcccgct gcccgcggga ttcgccgacg acgctgcgta
     1441 cctcaccgac ctcacgtggc ggggtgccgc cgagcgctgg ggcgacgagc tgccgccgga
     1501 cgtggccgag cggctcggct acgagctcaa ggtcatctgc gacatggggt tcgcgtcgta
     1561 tttcctgatc acgtgggacc tgatcaagta cgccaaggac cgcggcatcc gcgtcggccc
     1621 gggccgcggg tcggcggcag gttgtgctgt cgcctatgcg ctgcggatcc ccgatctcga
     1681 tccggtgcgc tacgacctgc tgttcgagcg cttcctcaac ccgagccgag tgtcgatgcc
     1741 cgacatcgac atggacttcg actcgcgata ccgcgacgag atgatccgct acgccgccga
     1801 acgctacggg cgcgatcacg tcgcgcagat catcacgttc ggcacgatca aggcccgcaa
     1861 tgccgtgcgc gacgcggccc gcgtcctcgg ctacccgtac ggcatcggcg acaagctggc
     1921 caaggcgatg ccgccgatgg tgatgggccg cgacacgcca ctcaagtact gcttcgagga
     1981 gaacccgaag tacgccgacg gttacaaggc ggcggccgac ctgcgggcga tgtacgacac
     2041 cgatcccgac ctgaagcggg tgatcgacgt cgccagaggc ctc
//
LOCUS       DATDBZ010000368         2360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2980917, whole genome shotgun sequence.
ACCESSION   DATDBZ010000368 DATDBZ010000000
VERSION     DATDBZ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2980917"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="VIX62_08835"
     CDS             complement(<1..1879)
                     /locus_tag="VIX62_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5860374.1"
                     /translation="MADVISTSAAPTLSSRAFRRPRLTTQLDAACERTITLICAPPGT
                     GKTTLAAGWAAGRPDLPVVWFDMEVRGNDLDPLYDELTERLEKVGQLASAHRAAHGGS
                     VSLADRLSRTRSGGADIVVVLDDAHEITDRPVWHDLNRLAVASPPWLHWIIVTRADPP
                     LGIQRLQLQGRLAQIRVSDLAFDVDEATTFMAWFGLDIPAHAVRTLVRWSEGWVAALC
                     LAARTMLSEGVETRPWERLGASEALVLDFLIEEVLDRLTPADRRFLLRTSAADLLTPE
                     LATLLTGNTMAGQRLHRLARSGTFLLQVDSSGTSYRYHGIMATLLRARLNDRMPAEAR
                     NLVSTAARWYGENGYLDEAERHAARIGDWHLVGQLRARRCSEHLIRTGALPAIPGNPT
                     SGPAAAGPAMRMLAIVEAMRQRDRRKTERVLAKGSESIDVSESPPSVKLLRELLLVER
                     SRWMPVHNGPARRSPMRRITHHVDNPVSRDALSSYVLVREGEALLAGGHEKAARRCFE
                     EVEVLSGASEWARREADAILAVMDAAAGDPDRATSMSRRSHSSGTSDAHVWEHLAAAI
                     AHGLRGEMAGLRHRTEALTSTDSPRSWLLEQCTRMLIGIATWRPPSIAPSIAPPPPGL
                     PS"
     gene            2063..>2360
                     /locus_tag="VIX62_08840"
     CDS             2063..>2360
                     /locus_tag="VIX62_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860375.1"
                     /translation="MAIDTFLSIVGVYESVDDALADYEAIKVLHREADLMDAYDAAVI
                     ERRADGKVKITKKHETPTRVGGVLGAGVGLATGLVVVLFPFAAIGSGVIATTTAG"
BASE COUNT          398 a          765 c          822 g          375 t
ORIGIN      
        1 aactggggag gccgggcgga ggaggcgcga tgctgggggc gatgctcggc ggccgccacg
       61 ttgcgatccc gatgagcatc cgcgtgcact gctccagtag ccacgaacga ggagagtcgg
      121 tcgaggtgag cgcctcggtg cgatggcgca ggccggccat ctcaccgcgg aggccgtgcg
      181 cgatcgcagc tgccaggtgc tcccagacgt gagcgtccga cgttccgctc gagtgagagc
      241 gccggctcat cgacgtggcg cggtccggat cgccggcggc ggcatccatc acggcgagga
      301 tcgcgtccgc ctcgcggcgt gcccactcgg acgccccgga caagacttcg acctcctcga
      361 agcagcgtcg cgcggctttc tcatgacctc cggcgagcaa ggcttcgccc tcgcgcacca
      421 ggacgtagga gctgagcgcg tcccgcgaga cggggttgtc gacgtgatgg gtgatccggc
      481 gcatcggcga gcgtcgagcc gggccattgt ggaccggcat ccatcgagat cgctcgacca
      541 ggagcagctc acgaagcagc ttcacggacg gcggtgattc ggacacgtcg atcgattccg
      601 atcccttcgc caacacccgc tccgtcttcc gacggtcccg ctgccgcatc gcttcgacga
      661 tcgcgagcat ccgcatcgcc ggacccgcgg ccgcgggacc cgacgtcggg ttgccgggga
      721 tcgcaggaag agcgccggtc cggatcaaat gttcactgca ccgtctggct cgaagttgcc
      781 cgacgagatg ccagtcgccg atccgagcag cgtgccgctc ggcctcgtcc agatagccat
      841 tttcgccgta ccagcgggca gcggtgctga cgaggttccg tgcctcggcc ggcatccggt
      901 cgttgaggcg tgcccggagg agtgtcgcca tgatgccgtg gtaccgatag ctggtgccgc
      961 ttgaatcgac ctgcagcagg aacgtgccag atcgcgcgag acggtggagg cgctgcccgg
     1021 ccatggtgtt cccggtgagc agcgtggcga gctcgggggt gagcaggtcg gccgcgctcg
     1081 tgcgcaggag aaagcgtcga tcggccggtg tgaggcggtc cagaacttcc tcgatcaaga
     1141 agtcgaggac gagcgcttcg ctcgcgccca aacgctccca aggacgcgtc tcgacacctt
     1201 ccgacagcat cgtgcgtgcc gccaagcaca gcgcggccac ccagccctcg gaccaccgga
     1261 ccaatgtccg cacggcgtga gccgggatgt ccagcccgaa ccaggccatg aacgtcgtcg
     1321 cttcgtcgac atcgaacgca agatcgctca cccggatctg ggccagccgt ccctggagtt
     1381 gcagccgctg aatgcccaac gggggatcgg ctcgggtcac gatgatccaa tgcagccatg
     1441 gaggcgatgc gacagcgagt cgattgaggt cgtgccacac cggtcgatcg gtgatctcgt
     1501 gagcgtcgtc gagcacgacg acgatgtcgg cacctcccga gcgcgttctg ctcagtctgt
     1561 cggcgaggga gacgctgccg ccgtgcgccg cccggtgcgc actcgcgagc tgtcccacct
     1621 tttcgagacg ctccgtgagc tcgtcgtaca ggggatcgag atcgtttccg cgcacttcca
     1681 tgtcgaacca gaccactggg aggtcgggcc gcccggccgc ccagcccgca gcgagcgttg
     1741 tctttccggt gcccggcgga gcgcagatga gggtgatggt gcgttcgcac gcggcgtcca
     1801 actgcgtcgt gagtcgcggt cggcggaagg cccgagacga gagcgtcggt gcggcggagg
     1861 tactgatgac atcggccatc gctgacatct ccgatccgat cgcgggggac gtgacgatca
     1921 aacacctgtg ggggtgaggc gcgaaccacc ctaatacgag agaatccccc gtgacgcggg
     1981 ccgtgcccgc gtgatcaccc ctcgtgggtg aggcatcagc gtggtcgtcc aagctgaata
     2041 atgaccaacc tgggaggttc caatggccat cgacacgttc ctgtcaatcg tcggggtata
     2101 cgagagcgtt gacgacgccc tcgccgacta tgaagcgatc aaggtgctcc accgcgaggc
     2161 ggacctgatg gacgcctacg acgcagctgt gatcgagcgg cgcgcggacg gcaaggtcaa
     2221 gatcaccaag aagcacgaga ccccaacgcg ggtgggtggt gtgctcggtg cgggggtcgg
     2281 gctggcgacg ggtctcgtcg tcgtgctgtt cccgttcgcc gccatcggga gtggggtcat
     2341 cgccacgacg acagcgggcg
//
LOCUS       DATDBZ010000369         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1560851, whole genome shotgun sequence.
ACCESSION   DATDBZ010000369 DATDBZ010000000
VERSION     DATDBZ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560851"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..1238
                     /locus_tag="VIX62_08845"
     CDS             453..1238
                     /locus_tag="VIX62_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5860376.1"
                     /translation="MSGAVEVVGRSESHNDHWVRAWPLRKPALVHLAIGGLLLLAVTS
                     VVGLLYMALLDGGPVGDADRAAAEWLEDRRTSTWNTLTDYGSMLSDTLVKVILVAIVG
                     GAMVLVWRRWHDGVFVALAVAVEATIFVISSFIVDRDRPPVEQLDPPAPSGSFPSGHT
                     AAAVAFYAGLFVVVCWHTRNRIVRAIFAVIAVTAPLIVGLSRVWRGMHHPLDVIAGLL
                     LGLATLYVVRGAVAKGVDEIDRRDPAAPERVRRLDLTSRGVPT"
     gene            1235..>2086
                     /locus_tag="VIX62_08850"
     CDS             1235..>2086
                     /locus_tag="VIX62_08850"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEY5860377.1"
                     /translation="MTSSTFTGETGSQTVEDLARRHPGLVMLGRLGWVAKGLVYGVVG
                     LLAVQIALDASGRDADNTAEQEASQTGAVTEIADTSFGEIALYVVAAGLLLYALWRLI
                     SVLLPAENSAKAWLTRVGYLASAAVYTALAWTALSFARQSGSSSGAPGEDAKVERFTR
                     ELMEKSGGRWLIGALGVLVIAIGIYFAIKGLQAKFRDELEPRGVGPIRHETIITLGRV
                     GWVGRAIVMGLVGWLLVRAAVRFRPDEAKGFDGALKEVTDSGLGVALVWFAAIALVLY
                     GLFCVISA"
BASE COUNT          281 a          698 c          725 g          382 t
ORIGIN      
        1 ggtccgccta tcgcaagatc ggcgtgacga ggcgctcggc cgctctgttg tggggcatcg
       61 accacgggtt ccaacccgac taccacagga tcgaccactg gcgcggcgga ccgtgagcaa
      121 gcgctgcatg ccgccgccct actgactctc gggccagcca tttccccctg aacccgtatc
      181 ccgtcggccc gccgaacccc aacgcgccgt tgggtcgccg ccccgagcgt ttcacttagc
      241 cgtgagtggg taccgcgctc atcgacatct tggggaggcc aggtgacgtc ggtgggtgtg
      301 tgggtgcacg aaggcgaggc cctgggacag gggcgggtgc ggttcaaccc cgtcacgatc
      361 gcgagaaccc gaccggaccg ggcgatgccc tcggagatcc acggtggctg gcggtccagc
      421 atcacgaagc tccccattcc ggtgccgtct tcatgagtgg cgcggtggag gtcgtcggcc
      481 ggtccgagtc gcacaacgat cactgggtgc gtgcgtggcc cttgcgcaag ccggcgctgg
      541 ttcacctcgc catcggtggg ctgctgttgc ttgccgtcac gtcggttgtc gggctgctgt
      601 atatggcgct tctcgacggc gggcctgttg gtgacgcgga tcgcgcggct gcggagtggc
      661 tcgaggaccg tcgcacctcg acgtggaaca cgttgaccga ctatggctcg atgctgtcgg
      721 acacgctggt gaaggtgatc cttgtcgcga tcgtgggtgg cgcgatggtg ctcgtctggc
      781 ggcggtggca cgacggggtg ttcgtcgcgc tggccgtggc ggtcgaggcg acgatcttcg
      841 tgatctcgag cttcatcgtg gaccgagacc gtcctccggt cgagcaactc gatccgccgg
      901 ctccctccgg aagcttcccg tctggtcaca ccgccgcagc ggtcgcgttc tacgccggtc
      961 tgttcgtagt ggtgtgctgg cacacgcgca accggatcgt gcgggcgatc tttgctgtca
     1021 tcgccgtcac cgcaccgctc atcgttggcc tctcgcgagt atggcgcggc atgcaccacc
     1081 cgctcgacgt catcgccggg ctcctgctcg gcctcgccac gctctacgtc gtgcgcggcg
     1141 ccgtggcgaa gggtgtcgat gagatcgacc gccgagatcc ggccgcgccc gagcgtgtcc
     1201 gacgattgga cctcacctcc cgaggagtgc cgacatgacc agttccacgt tcaccggtga
     1261 gaccggatcg cagaccgtcg aagacctcgc gcgtcgccat cccggtctgg tcatgctggg
     1321 caggctgggg tgggtcgcga agggactcgt ctacggtgtg gttgggctcc tcgccgtgca
     1381 gatcgcgctc gatgcgtcgg gccgcgatgc cgataacacc gccgagcagg aggccagcca
     1441 gaccggcgcc gtcaccgaga tcgccgacac atcgttcggc gagatcgcgc tctacgtcgt
     1501 cgccgccggg ctccttctct acgccctctg gcggctcatc tccgttctgc tgccggccga
     1561 gaactccgcg aaggcctggc tcaccagggt gggctacctc gccagcgccg cggtctacac
     1621 cgcgctggca tggacggcgc tgtccttcgc cagacagagc ggctcgtcga gtggcgcacc
     1681 cggcgaggac gcaaaggtcg agcgcttcac gcgcgagctg atggagaaga gcggggggcg
     1741 gtggctgatc ggcgcgctcg gtgtcctcgt catcgccatc gggatctact tcgcgatcaa
     1801 ggggctgcag gcgaagttcc gcgatgagct cgagccacgc ggcgtcggcc cgatccgcca
     1861 tgagacgatc atcacgctcg gccgcgtcgg ctgggtcggt cgggccatcg tcatgggcct
     1921 cgttggctgg ctcctcgtcc gagccgccgt gcggttccgc cccgacgagg ccaaggggtt
     1981 cgacggtgcg ctgaaggaag tcaccgactc cgggctcggc gtcgccctcg tgtggttcgc
     2041 ggccatcgcg ctcgtgctgt acgggctgtt ctgcgtcatc tcggcg
//
LOCUS       DATDBZ010000370         3759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1344792, whole genome shotgun sequence.
ACCESSION   DATDBZ010000370 DATDBZ010000000
VERSION     DATDBZ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344792"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VIX62_08855"
     CDS             <1..284
                     /locus_tag="VIX62_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860378.1"
                     /translation="ATDPDRQRQGLASAAIAPVLERADHDRVTTYLETGAPENLAFYA
                     RAGFEVTDEVVLPDGPAVWGLQRSPWISDRLGTKEQGMSAEGIGRIEEP"
     gene            complement(214..1407)
                     /locus_tag="VIX62_08860"
     CDS             complement(214..1407)
                     /locus_tag="VIX62_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017830053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5860379.1"
                     /translation="MDPRSNPYTPNAGVRPRAFAGRDEDLEAFDVMLDRLLRGYADQS
                     MLITGLRGVGKTVLLGAFAEQAHMAGWVSLDAEISPEAPFGPRMAQLVRRAVLELAPR
                     DRWLDRLRRAAGVLRSFSITVSSDGAVTGSIDVEPLEGQADSGELGEDLTDLLVALGE
                     AAQEQETGVVFLFDEVQFLSKQEFEALIRALHKTTQRQLPISLVGAGLPHLPRLAGEA
                     KSYAERLFRFRTLGPLSVPDAIDALTQPANELGVSFEERAVDTVVVYTDGYPYFIQEY
                     GKVVWDRAEGSPITAVEAVESQAIVEARLDESFFQVRIQRASEQETEYLRAMAELGAA
                     PQRAADVAEVLERPSGELGKVRTRLIEKGLLYSPKYGYAAFTVPQFDRFLRRSFPAPS
                     SRADR"
     gene            complement(1486..2277)
                     /locus_tag="VIX62_08865"
     CDS             complement(1486..2277)
                     /locus_tag="VIX62_08865"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEY5860380.1"
                     /translation="MWTEADGPAPALYLGHGAPPLLDDELWTSQLATWAKDLPKPTAI
                     LIVSAHWESAPLTLGATETGVPLTYDFYGFPERFYRMTYRSPGAPELAARVRALMPDT
                     ETVADRPGYGLDHGAYVPLMVMYPDADVPVLQISMPTLDPQRLFELGQRLRPLRDEGV
                     LIVGSGFLTHGLPFLRDFRPDADPPGWSREFDAWSAEALARGDVDELMAFRDRAPGMP
                     YAHPTVEHFAPLFVTLGAASDAERAPDTEIEGFFLGLAKRSFQIA"
     gene            2439..3389
                     /gene="pip"
                     /locus_tag="VIX62_08870"
     CDS             2439..3389
                     /gene="pip"
                     /locus_tag="VIX62_08870"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY5860381.1"
                     /translation="MTGLHPRIEPYDAGMLSVGDGHQVYWETCGDPNGRPAVVVHGGP
                     GSGCTGWHRRLFDPATTRIVLFDQRNCGRSRPHASEPDVDLSTNTTQHLIADMEALRE
                     HLEVERWLVFGGSWGSTLSLAYAEAHPDRVSEMVLWGITTGRRAEADWLFRGGVAPLF
                     PEQWERLCAGVPASDRDGDIVEAYARMLHDPDPAVHRRAALDWCTWESATPDWPPTAG
                     LDSRYTDPDFALAFARLVTHYIRHDEFLEDGVLLRDAGVLADIPGTLVNGRFDLQAPI
                     ANAWALHRVWPRAELVIVDDAGHAADEPALTDALIRALDR"
     gene            complement(3420..3620)
                     /locus_tag="VIX62_08875"
     CDS             complement(3420..3620)
                     /locus_tag="VIX62_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860382.1"
                     /translation="MIAAVSFFWWLIFFVLWVVFLTITMSIARAKGHSPLLWGLLACV
                     LPGITVIIVLLLPVRTQGDAAP"
BASE COUNT          630 a         1276 c         1278 g          575 t
ORIGIN      
        1 tggccaccga tccggatcgc caacggcagg gactggccag tgctgcgatc gcgccggtgc
       61 tcgagcgggc cgatcacgat cgggtcacca cgtacctgga gacgggcgct ccggagaacc
      121 tcgccttcta cgcccgcgcc ggcttcgagg tcaccgacga agtggtcctg cccgacggtc
      181 ccgccgtgtg gggccttcag cgatctccgt ggatcagcga tcggctcggg acgaaggagc
      241 agggaatgag cgccgaagga atcggtcgaa ttgaggaacc gtgaacgccg cgtacccgta
      301 cttcgggctg tacaggaggc ccttctcgat gaggcgggtg cgcaccttgc ccaactctcc
      361 ggacgggcgc tcgagaacct ccgcgacatc ggcggcgcgt tgcggcgccg ccccgagttc
      421 ggccatcgcc cggagatact cggtctcttg ttcgctggct cgctggatgc gcacctggaa
      481 gaagctctcg tccagtcggg cctcgacgat cgcctgggac tcgacggctt ccaccgcggt
      541 gatgggcgac ccctccgctc gatcccacac gaccttgccg tactcctgga tgaagtacgg
      601 atacccgtcg gtgtagacca cgacggtgtc gacggctcgc tcctcgaacg agacgccgag
      661 ctcgttcgcg ggctgcgtga gggcatcgat cgcatcgggc accgacagag ggccgagcgt
      721 tcggaagcgg aacagtcgct cggcgtatga cttcgcttcg cccgccaacc tggggaggtg
      781 cggcagaccg gcaccgacca gggagatcgg gagttgccgt tgcgtcgttt tgtgcagcgc
      841 gcggatcagg gcttcgaact cctgcttcga caggaactgg acctcgtcga agaggaacac
      901 gacgccggtc tcctgctcct gcgcggcctc ccccagcgcc accaacaggt cggtgaggtc
      961 ctccccgagt tccccactgt ccgcctgacc ttcgagcggc tcgacatcga tggagccggt
     1021 caccgccccg tccgaggaca cggtgatgga gaacgaccgc aggacgccgg ccgctcgccg
     1081 caaccgatcg agccaacggt ccctcggcgc cagctcgagc accgctcgac gaacgagctg
     1141 agccatcctg ggtccgaagg gtgcctcggg cgatatctcg gcatcgagcg aaacccaccc
     1201 ggccatgtgg gcctgttcgg cgaacgcccc gagcagcacg gtcttgccga cccctcgcag
     1261 gccggtgatg agcatcgact ggtcggcgta gccgcggagc agacgatcga gcatcacgtc
     1321 gaatgcctcg aggtcctcat cccggccggc gaaggctctc ggccgtaccc ctgcgttcgg
     1381 cgtgtaggga ttggagcggg gatccatcgc gtaaagtttg tctagttggg cggactttgt
     1441 caactctcga caaactctgc ccaagctgtc aaacctaggc ccgggtcacg cgatctggaa
     1501 cgagcgcttc gccaggccca ggaagaagcc ttcgatctcg gtatcggggg cacgttcggc
     1561 atcgctcgcc gctcccagcg tgacgaatag cggggcgaaa tgctccacgg tgggatgcgc
     1621 gtacggcatg ccgggcgcgc ggtcccggaa cgccatcagc tcgtcgacgt cgccacgcgc
     1681 caacgcttcg gcgctccatg cgtcgaactc ccgcgaccag cccggcggat cggcgtccgg
     1741 gcggaagtcc cgcaggaacg gcagtccatg cgtgaggaag cccgacccca cgatgagcac
     1801 accctcgtcg cggagcggtc gcaggcgctg gccgagctcg aacagacgct ggggatccaa
     1861 cgtcggcatg gagatctgca acacgggcac gtccgcatcg gggtacatca ccatgagcgg
     1921 cacgtacgcc ccgtgatcca ggccgtagcc gggccggtcg gcgaccgtct cggtgtcagg
     1981 catcagcgcc cgaacgcgcg cggcgagctc cggtgcgccc ggtgaccggt aggtcatgcg
     2041 gtagaagcgc tcggggaagc cgtagaaatc gtaggtgagc ggcacgccgg tctcggtggc
     2101 gcccagcgtg agcggcgccg attcccagtg cgccgacacg atcaggatcg ccgtgggctt
     2161 cgggagatcc ttcgcccacg tcgcgagctg tgaggtccac agctcgtcgt cgaggagtgg
     2221 cggcgcgcca tggcccaggt agagcgcggg cgccggaccg tcggcctccg tccacatgcg
     2281 gctgttcgcg tgcccgaggt cttgcggttg gttcgtcacg tcgtcgtgaa ccctcgcacc
     2341 cggtgggata tttcgcagtg gttcccaacg agaccgatca cggtcccgtc gccgccctgc
     2401 acagagccta gctgtcccat catcgatacg ctgcaccgat gacgggccta catccgagga
     2461 tcgagcccta cgacgccggc atgctctcgg tcggtgacgg ccaccaggtg tattgggaga
     2521 cctgcggcga cccgaacggc cgccccgcgg tcgtcgtaca cggcggtcca gggtcggggt
     2581 gcaccggttg gcaccggcgg ttgttcgatc cggcgaccac ccgcatcgtg ctgttcgatc
     2641 agcgcaactg cggccggagc cggccacacg cgagcgagcc cgacgtcgat ctgtcgacga
     2701 acacgacgca acacctgatc gcggacatgg aggcactccg ggaacacctg gaggtggagc
     2761 gctggctggt cttcggaggc tcgtggggca gcacgttgtc gctcgcgtac gccgaagcac
     2821 accccgaccg ggtgagcgag atggtgctct gggggatcac gacgggccgg cgggccgaag
     2881 ccgactggct gttccgcggc ggcgtggcgc ccctgttccc cgaacaatgg gaacgcctgt
     2941 gcgccggggt gccggcctcc gaccgagacg gcgacatcgt cgaggcctac gcacggatgc
     3001 tgcacgaccc cgacccggcg gtccaccgcc gcgctgcgct cgactggtgc acgtgggagt
     3061 cggccacccc tgactggcca cctaccgcgg gactggacag ccggtacacc gatcccgact
     3121 tcgccctggc gttcgcccgc ctggtgaccc actacatccg gcacgatgaa ttcctcgaag
     3181 acggggtgtt gctccgcgac gccggcgtgc tcgccgacat cccgggcacg ctggtgaacg
     3241 gccgcttcga tcttcaggcg ccgatcgcga atgcatgggc ccttcaccgg gtgtggccgc
     3301 gggccgagct cgtgatcgtg gacgacgctg ggcatgccgc cgatgagccg gcactcacgg
     3361 atgcgctgat ccgtgccctc gaccgctagg gcacggaccc gatccgggga cgtgcggacc
     3421 tacggggcag cgtcgccctg ggtgcggacc gggagcagca gcacgatgat gaccgtgatc
     3481 cccgggagga cgcaggcgag cagaccccac agcaaggggc tgtgaccctt ggcgcgagcg
     3541 atcgacatgg tgatggtcaa gaagacgacc cagagcacga agaagatcaa ccaccagaag
     3601 aacgacactg ctgcgatcac gtggtcccct ttgttcgacg atggccctcg ggccggagca
     3661 gacactagcc catcgaggag tcggtccgcc cgagagcgct cgtgccgagc cgatcgcggc
     3721 ccgtgcgtcg agcaccgatg aaggcatcga gcgtccgct
//
LOCUS       DATDBZ010000371         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_100432, whole genome shotgun sequence.
ACCESSION   DATDBZ010000371 DATDBZ010000000
VERSION     DATDBZ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100432"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /gene="mftD"
                     /locus_tag="VIX62_08880"
     CDS             <1..1091
                     /gene="mftD"
                     /locus_tag="VIX62_08880"
                     /EC_number="1.4.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015889864.1"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the
                     non-exchangeable NADH of TIGR03971 family SDR-type
                     oxidoreductases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="HEY5860383.1"
                     /translation="KGITYADNVQAFGEIGLAPHVVGHQSPRDLSTTVMGIPVALPVL
                     ISPTGVQAVHPDGEVAVARASANRGTIMGLSSFASKAVEEVTAANANTLFQVYWSGTR
                     EAMVQRLDRARSAGAKGLIATLDWSFSHGRDWGSPSIPERMNVKTMVRFAPNVLPHPR
                     WLWSFAKTGRIPDLTAPNLTPPGGQAPTFFEAYYEWMQTPPPTWEDIAWLRDEWGGPF
                     MLKGVGRVDDARRAVDLGVDALSVSNHGGNNLDATPASIRLLPAIVDEVGDQAEVLLD
                     GGIRRGSDVAKALALGARAVMIGRAYLWGLAANGQAGVENVLDILRNGLDSAVLGLGH
                     ASVGGLNPGDLDIREGFARSLGASSAVR"
     gene            1100..1792
                     /gene="mftE"
                     /locus_tag="VIX62_08885"
     CDS             1100..1792
                     /gene="mftE"
                     /locus_tag="VIX62_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="HEY5860384.1"
                     /translation="MQRLDTLAWPDIPAAPVVFVPLGATEQHGPHLPFDTDTVIAEAV
                     STRLDAAAIVAPVLAYGSSGEHQDFPGTISIGHSALRMMLVELVRSTAHWAGRVIIVN
                     GHGGNIPCLSSVVPELIAEGHDVAWLPCATSAGDAHAGVTETSIMLHLAPDRVRLDRA
                     EGGPKTELALLLRKLKRSGVRAVSPSGVLGDPTGATAEHGRRCLDEMVAGALRRFEGG
                     RLDDRGCLTEAA"
     gene            1789..>2081
                     /locus_tag="VIX62_08890"
     CDS             1789..>2081
                     /locus_tag="VIX62_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system glycosyltransferase"
                     /protein_id="HEY5860385.1"
                     /translation="MTLPLPLGFVVELNVRTRVRDGGRTLIGGSPTRLLYLSPTARRM
                     LVNGRVTVTDPESAALADRLLETGIADPVVNALPDLDPGLISWVIPVRDRPEGL"
BASE COUNT          316 a          731 c          682 g          352 t
ORIGIN      
        1 agaagggcat cacctacgcg gacaacgtgc aggccttcgg tgagatcggt ctcgcgccgc
       61 acgtcgtcgg acaccagtcg ccgcgcgacc tgtcgaccac cgtgatgggg attccggtcg
      121 ctctgccggt gctcatctca ccgacgggcg tgcaagccgt gcaccccgat ggcgaggtcg
      181 ccgtcgctcg agcatctgcg aaccgtggaa ccatcatggg cctgagctcg ttcgcgtcga
      241 aggccgttga agaggtgacc gctgcgaacg cgaacaccct tttccaggtc tattggagcg
      301 gcacgcgcga ggccatggtg cagcgcctcg accgagcgcg atcggcggga gcgaagggtc
      361 tcatcgccac gctcgactgg tccttctcgc acggccgcga ttggggaagc ccctccatcc
      421 ccgaacggat gaacgtcaag acgatggtgc gattcgcccc caacgtcctg cctcacccgc
      481 gctggctgtg gagcttcgcg aagacgggcc ggatccctga tctgacggcg cccaatctca
      541 ccccgcccgg cgggcaggcg ccgacgttct tcgaggccta ctacgagtgg atgcagaccc
      601 cgcctcccac ctgggaggac atcgcctggc tccgcgatga gtggggaggt ccattcatgc
      661 tgaagggtgt cggtcgcgtc gatgatgctc gtcgcgccgt cgacctgggc gtggatgcgc
      721 tgtcggtgtc caatcacggc gggaacaacc tcgatgcgac tccggcttcc attcggctgc
      781 ttcccgcgat cgtcgatgag gtcggcgacc aggccgaagt gcttctggat ggcgggatcc
      841 gccgcgggag cgacgtcgcg aaggcccttg cgctcggagc ccgggcggtc atgatcgggc
      901 gggcatacct gtggggtctg gcggccaatg gccaggccgg cgtcgagaac gtgctcgaca
      961 tcctccgcaa cggtctcgat tccgccgtgt tgggcctggg ccatgcctcc gtcggcgggc
     1021 tcaatccggg tgacttggac attcgggaag gcttcgcacg gtcgttgggt gcctccagcg
     1081 cggtcaggta gctctgcgga tgcaacgcct cgacaccctc gcctggcccg acatacccgc
     1141 tgcaccggtc gtcttcgtgc cgctgggcgc gacagagcaa catggccccc acctgccgtt
     1201 cgacaccgac accgtgatcg ctgaggcggt atccacacgc ctcgacgccg cggcgatcgt
     1261 cgctcccgtt ctcgcgtacg gctccagcgg agagcaccag gacttccccg gcaccatttc
     1321 gatcggacat tccgccctgc gtatgatgct ggtcgaactc gtccgctcga ccgctcattg
     1381 ggccgggcgc gtgatcatcg tgaacggcca cgggggcaac atcccctgcc tttcctcagt
     1441 ggttcccgaa ctcatcgccg agggacatga cgtcgcctgg ctgccctgcg ccacctcggc
     1501 gggcgacgcc cacgcgggcg tcaccgagac ttcgatcatg ctgcatctcg cgcccgatcg
     1561 tgtccggctg gaccgagcag aaggcgggcc caaaacagag ctcgcgcttc tcctgcggaa
     1621 actgaagcga tcgggggtca gagccgtttc tccgtcgggg gtgctcggcg acccgacggg
     1681 cgcaaccgcg gagcacggtc ggcgctgcct cgacgaaatg gtcgccggcg ccctccggcg
     1741 attcgaaggg ggccgcctgg acgatcgcgg atgcctcacg gaagccgcat gacgctgccc
     1801 ctgccgctcg gcttcgtcgt cgagctcaac gtccggacgc gggtgcgaga cggcggccgc
     1861 acactgatcg gcgggtctcc gacccgcctg ctgtatctct ccccgaccgc ccggcggatg
     1921 ctcgtcaacg gtcgcgtgac cgtcaccgat cccgagagcg cggcactcgc agaccggctg
     1981 ctcgaaaccg gcatcgcaga ccccgtcgtg aacgccctgc ccgatctgga tcccggtctc
     2041 atctcctggg tgatccccgt ccgcgatcgc cccgaggggc t
//
LOCUS       DATDBZ010000372         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2580496, whole genome shotgun sequence.
ACCESSION   DATDBZ010000372 DATDBZ010000000
VERSION     DATDBZ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2580496"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VIX62_08895"
     CDS             complement(<1..513)
                     /locus_tag="VIX62_08895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860386.1"
                     /translation="MELAVCVTDDEYEAWRDVRLAVEADSRTLTVEELREQGSSDRLL
                     LLAIEGGDVVGSGIADRAETARAGFVAPRVLPGRRRLGVGSALLRVLAEHCSGLGLST
                     VRTNVDDAGSLAFAERYGFVEVDRQVEQVRAVGDEPAPSDLPAGVEVIEARERPELWK
                     ACFDTFGTEVL"
     gene            complement(2321..2403)
                     /locus_tag="VIX62_08900"
     tRNA            complement(2321..2403)
                     /locus_tag="VIX62_08900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2368..2370),aa:Leu,seq:tag)
     gene            2473..>2732
                     /locus_tag="VIX62_08905"
     CDS             2473..>2732
                     /locus_tag="VIX62_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860387.1"
                     /translation="MAPRRTIAACFAILALLGAGCGEFPDGASALEGAQAAAASPSTT
                     PSPSPREFLEIGEQAASHDAGEPAGATVPAPRSLERWGWALRS"
BASE COUNT          440 a          924 c          922 g          446 t
ORIGIN      
        1 gagcacctcc gtgccgaacg tgtcgaaaca ggccttccag agttcggggc gttcgcgcgc
       61 ctcgatcacc tcgacgccgg ccggcagatc tgacggggcc ggttcgtcgc ccaccgcgcg
      121 cacctgttcg acctggcggt cgacctcgac gaatccgtac cgctcggcga acgcgaggga
      181 gcccgcatcg tcgacattgg tccggaccgt gctcaggcct aggccgctgc agtgttcggc
      241 taggacccgc aggagcgccg accccacgcc caggcgacga cgcccgggca gcacgcgcgg
      301 cgcgacgaat cccgcacggg cggtctcggc ccgatcggcg atgccggagc ccaccacgtc
      361 gccgccctcg atcgccagca gcagcagacg gtccgacgag ccctgctccc gcaactcctc
      421 gacggtgagt gtgcgcgagt ctgcctcgac ggcgagccgg acatctcgcc acgcttcgta
      481 ctcgtcgtcg gtgacacaga cggccagctc catggatcgc aatgtagtcg gcgaggccgg
      541 gtccaaacct cctgagccgc gactggagcc gcgcggtgct tgcatggacg ccgtcggtgc
      601 ccgataccgt cgtgtgatgg agccgacccc gcctcagccc gttcgcacca cgtcacgatc
      661 aacgccggaa cgatgatggt cggctcggcg gaactgcgca tcttctggaa aggcgattgt
      721 ccttcagccg tcttggttcg ctcagcgtac gagtggcctg ccgcagagat ccggacacga
      781 tcgctgaagt ctggttgatc gaggctcggc tggcataccc cggttcaaca ctgcgctggt
      841 gctcggtgac gatggtcctg tcacgaccgt tgccgtcctg gttggcgacg ccggtcctta
      901 cgaacgctgg cgagcgatgc actgggtcag gacggggtgg ttcggcaccg agcccatgaa
      961 cgcgttcgag accagtcgcg tttccgtcca gtcgcaagag gacgtagcct tggcccgacg
     1021 aagtcgagtt gtggactacg gtcgtcacgt ggccgtcgaa caccatcgcc ggaaacacgc
     1081 gcgccaccag cggctcgggg cagcctcgtt cggtctcgcg ttcgtcatgg cggtgggatg
     1141 gctgatcctg agcacgatcc gatcggcacc tcccgtcccc accgatccat ccgcaaccca
     1201 gagcgaactc ggcgtcttcg ctgatgttcg gggatgggtg acgtacggcg acgtcggcga
     1261 ggactacttc ggatatggga tctgggcggt cgatccggcg aaccccgatg agcgcccggt
     1321 gctgctgagc cgcggcgcag ggctcccgat cgcgtggtcg cgcgacggct cgaagctgct
     1381 ctccaatcga ggtgggccct tcttccagcg tcgcgactca ctcgtcgtgg tgacctccga
     1441 tggcacgaag acggtggtcg cccgcgccga ggagatcacg ggtgggtcgt tcactcccga
     1501 cggatcggcg gtcgtctacg gagtctcgga cgggaaggac gtgaagtggg gcatctacat
     1561 cgtcgatgcg aagggcggaa cgcctcagct catctacgct ccgggtcgcg ggcccgcacc
     1621 caacccgtgg aagatggcgg tgtactcacc aaccctctca cccgacggct cacggctcgc
     1681 gtacgtcgaa ggaaaggggt cgtctgggca cagcctctgg gtgacggacg tcgatgggac
     1741 cgaccggcac cggatcgtca gagatgaagt gcggatcgac atctactcgg agacgaacct
     1801 gcagtggtcg cccgatggga cacggttggt gttcggcgga ggaggcatct atgtcgtcaa
     1861 tgacgacggg tcagcactca tacgcgtcgc cagggggcgg gagaccgcgt actggtcgcc
     1921 tgacggctcg cggatcgcct tccagcgatt cgggacgttc tacaccatgg cgccggacgg
     1981 tagcgacatc cgtagcttcg gggtacggcc tcgagcgacg gaatcggtcg ttggaccgtg
     2041 gaatccgctc gtgccggctg cctcaacttc gctgctctcc tcgtatgacg ccgtcgagga
     2101 accaggcagc tggcagagtt ccccgatcac gtggtcgaag ggacgctccc cgtcccgcac
     2161 gggggacact agacgcactg ctccgctccc tcgagaggcc atttcggcat cgaaccccaa
     2221 ccggcagccg cgaccttctg tgagccagcg tgagcaagac gggggttctc gcgagatctc
     2281 agagaagggc tcagaacgca gttctgcagg tcagaggggg tgcgagaggg gggagtcgaa
     2341 cccccacgcc ctttcgggca ccgggaccta aacccggcgc gtctgccatt ccgccactct
     2401 cgccagtcgg gcaaggctaa cccgtgtccc cgcctacgca cgttcctcgt ccatctggaa
     2461 tgatggaccg cagtggcccc ccgacgcacc atcgccgcct gcttcgccat cctcgcgctc
     2521 ctgggcgcgg gctgcggcga gttccccgac ggcgcctcgg cgctcgaggg tgcccaggcc
     2581 gcggcggcat ccccctccac gacgccctcg ccgtcgccgc gggagttcct ggagatcggg
     2641 gagcaggcgg catcgcacga cgccggcgag ccggccgggg cgacggtgcc ggcgccgagg
     2701 tcgctcgagc gctgggggtg ggcgctgcga tc
//
LOCUS       DATDBZ010000373         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2581198, whole genome shotgun sequence.
ACCESSION   DATDBZ010000373 DATDBZ010000000
VERSION     DATDBZ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581198"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="VIX62_08910"
     CDS             complement(<1..1354)
                     /locus_tag="VIX62_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEY5860388.1"
                     /translation="MTGDHFEGSGTNELDGLLLDAKLSIPQPRQAAVSRASIISSCRA
                     SARRVVAVTAPAGYGKSTLLAQWADTEDRRVAWVSLDRLDDDPMVLLSTLASAYALVS
                     PEDADLISDVGGLGTSALGRAAPRLASVLRTSPAPFVFMLDDLHELRSPACHDVLGVV
                     IAGMPLGSQFVATSRSEQPHIARLRASGDALELAAEDLALDPAGAKQIFAEASVSLSD
                     DSAATVIERTEGWPVGLYLASLIASDGKADALAVSGDDRYVADYLYRETVMQLPEETQ
                     RFLRRTAMLDQVSGSLCDAVLETTGSHEMLRNLERSNTFLIPLDRRRDWYRYHALFRE
                     FLEGELGRVEPDIVMTLHLRAADWYEANGSPAIALHHLLSTDERERCVHLATALTLET
                     YAAGQMSTVQRWLWALGNDAIEGYPPLAVLAGWVTAMTGQTADAQRWAAIVDATTFDL
                     T"
     gene            1493..1891
                     /locus_tag="VIX62_08915"
     CDS             1493..1891
                     /locus_tag="VIX62_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860389.1"
                     /translation="MSEERSSGAVGWTMFAGVMMILIGAFHAIAGLAGILEDDFYAVT
                     PNYVLEFDATTWGWVHLIGGVIVLFAGFSVFKGAVWARTVGVIIAAVSALVSFAWIPF
                     YPVWSIVVIAIDVAVIWALTAHGRDITMVE"
     gene            1896..>2325
                     /locus_tag="VIX62_08920"
     CDS             1896..>2325
                     /locus_tag="VIX62_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019070060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEY5860390.1"
                     /translation="MTTTTSAHPTVEERKAQGKGLRASADPADHKRWGPAGDRPDPVT
                     LFEEQNARREPDLVPVRHGRMMASPFTFYRGAAKIMAADLEHTPRAGLTAQLGGDAHL
                     SNFGLYASPERALVFDLNDFDETLPGPFEYDVKRMSASFTI"
BASE COUNT          392 a          753 c          799 g          381 t
ORIGIN      
        1 gggtcaggtc gaaggttgtg gcatcgacga tcgccgccca tcgctgggcg tctgccgtct
       61 gccccgtcat cgccgtcacc cacccggcca agacggcgag tgggggatac ccctcgatcg
      121 cgtcgttgcc gagcgcccac agccaccgct ggaccgtcga catctgtccg gccgcgtatg
      181 tttccagcgt caaggcagtc gcgaggtgca cacagcgctc tcgttcgtcc gtactcagca
      241 ggtggtgaag ggcgatcgcc ggggaaccat tcgcttcgta ccagtcggcg gcacgcaggt
      301 gaagcgtcat gacgatgtcg ggctcgaccc gaccgagttc gccttccagg aactcccgga
      361 acagggcgtg gtagcggtac cagtcccgcc ggcgatcgag cgggatcagg aacgtgttgg
      421 atcgctcgag gttccgcagc atctcgtgcg atcccgtcgt ctcgaggacc gcatcgcaga
      481 gcgagccgct cacctgatcg agcatcgccg tccgtcgaag gaagcgctgc gtctcctccg
      541 gcagttgcat cacggtctcg cgatagaggt agtcggccac gtaccggtcg tcacccgaga
      601 ccgcgagggc gtcggccttc ccgtcgctcg cgatcaacga cgcgaggtag agaccgaccg
      661 gccacccctc ggtccgttcg atcactgtgg ctgccgagtc gtccgaaagg ctaacgctgg
      721 cctccgcgaa gatctgtttg gcgccggcgg gatcgagagc gaggtcctcc gccgcgagtt
      781 ccagcgcatc ccccgatgct cggagtctcg cgatgtgtgg ttgttcggaa cggctggtgg
      841 cgacgaactg cgagcccaga ggcatgcccg cgatcaccac gcccagcacg tcgtgacacg
      901 caggcgatcg aagctcgtgc aggtcgtcga gcatgaacac gaacggcgct ggactcgtcc
      961 gcaacacgga cgccaggcgc ggagcggcgc gtccgagtgc cgacgtgccg aggcccccca
     1021 cgtcggagat caggtcggcg tcctcgggtg agaccagggc gtacgcggat gcaagtgtgg
     1081 agagcaggac catcggatcg tcgtcgagtc ggtccagtga cacccaggcc acgcgacgat
     1141 cctcggtgtc cgcccactgc gccagcaggg tcgacttgcc gtaacccgcc ggcgccgtca
     1201 cggccaccac acggcgagcg ctcgctcgac acgaggagat gatcgaggcc cggctgacgg
     1261 ccgcctgacg cggttgcggg atcgagagct tggcatcgag cagcagtccg tcgagttcgt
     1321 tggtgcctga tccctcgaag tgatccccgg tcaccggcga atcctagggg gtgccgagaa
     1381 ggccctcaac tcgccgaatc acgcaccctg cgtgatgtga cccagtcccc gttcggtgtt
     1441 ggatgcgtga tgcagccaga gggcaggggc cgaggagtcg aggggggaag gcatgtctga
     1501 agagcgaagc agcggggcgg tcggttggac gatgttcgca ggggtaatga tgatcctgat
     1561 cggcgcgttc cacgcgatcg cggggcttgc aggcatcctc gaggacgatt tctacgccgt
     1621 cacgccgaac tacgtgctcg agttcgacgc gacgacgtgg gggtgggtcc acctgatcgg
     1681 cggcgtcatc gtgttgttcg caggcttcag cgtgttcaag ggggcggtgt gggcgcggac
     1741 ggttggcgtg atcatcgcgg cggtgagcgc gctcgtgagc ttcgcgtgga tcccgttcta
     1801 tcccgtgtgg tccatcgtcg ttatcgcgat cgatgtcgcc gtgatctggg cgctgacggc
     1861 ccacggccgc gacatcacga tggtcgagtg agcacatgac aacgaccacg agcgcgcatc
     1921 ccaccgtcga ggagcgcaag gcgcagggga agggcctgcg agcgtccgcc gatccggccg
     1981 atcacaagag atggggaccg gcgggcgacc ggcccgaccc cgtgacactc ttcgaggagc
     2041 agaacgcgag gcgtgagccc gacctcgtgc cggtgcggca cggtcggatg atggcctcgc
     2101 cattcacctt ctaccgcggg gccgcgaaga tcatggccgc tgacctcgag cacaccccgc
     2161 gagcggggct caccgcccag ctcggcggcg acgcccacct ctcgaacttc gggctgtacg
     2221 catcgcccga acgggcgttg gtcttcgacc tcaacgactt cgacgagacg ttgcccggcc
     2281 cgttcgagta cgacgtgaaa cgcatgtcgg cgagcttcac gatcg
//
LOCUS       DATDBZ010000374        10696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1345421, whole genome shotgun sequence.
ACCESSION   DATDBZ010000374 DATDBZ010000000
VERSION     DATDBZ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345421"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2223)
                     /locus_tag="VIX62_08925"
     CDS             complement(<1..2223)
                     /locus_tag="VIX62_08925"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HEY5860391.1"
                     /translation="MPEVTGSDAPRQVRRALARADAGKALTLDEATSLMAARGEELDR
                     LMGLAARLRDLEHGEVVTYSRKVFIPLTMLCRDHCHYCTFAKPPAKLDTPFLTPEEAV
                     SIADAGRRMGCKEALFTLGDRPEERYDVAREWLEARGYASTLDYVRAVAIRVIEETGL
                     LPHLNPGVMSYEEIARLKQVSASMGMMLETSSMRLAERGGPHFGSPDKVPAVRLRTIE
                     DAGRLAVPFTTGILVGIGETDRERAESLFAIRDAHRRYRHIQEVIVQNFRAKPGTAMQ
                     AAPEPDDEAFLAAVATARVVLGPRMHLQAPPNLSDPDQQVRLLDAGIDDWGGVSPLTP
                     DHVNPEKPWPSIEALAATTASRGKQLRERLTIYPAFAAKPDPYLAGKMRAPVMALLGP
                     DGRAVEGRHPEPIAWQDPDVQWKPRTIALTFAKADGTGLRGDADEVYGDADTPETTRA
                     WSKREIHPERIDRDIVAALAAAEAHRPISDEHALALFQAEGAALDALCRVADRLRAEA
                     VGDDVTYVVNRNINFTNVCYVGCRFCAFAQREMDAESYTLTLAEVADRAEEAWELGAT
                     EVCMQGGIHPDLPGSFYFDLLDAVKARTPGMHIHAFSPMEVMNGSTKLGLSYAEFLAE
                     AKQRGLGTIPGTAAEILDDEVRWALTKGKLPADTWEEIIRTAHGLGIRSSSTIMFGHV
                     DAPPHWVGHLRRLARIQADTGGFTEFVPLPFVHQHAPIYLAGKARPGATSAEQLRMHA
                     V"
     gene            2253..3200
                     /gene="cofD"
                     /locus_tag="VIX62_08930"
     CDS             2253..3200
                     /gene="cofD"
                     /locus_tag="VIX62_08930"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEY5860392.1"
                     /translation="MKVVALAGGIGGGKFLRGLIRAVPPNDVIAVVNTGDDLWMHGLR
                     ICPDLDSVTYWLGDAFDRERGWGRRDETFRATEELRRFDPEAAWFGLGDLDLATHLYR
                     TRLLADGRSLSEITGLVAARFGIAARILPMSDDDVTTRVDVVSFGQEMDLHFQEYWVQ
                     RGGRDDVKGVRFQGLDAARPAPGVLEALAEADAIVFAPSNPVVSIGPILGVPGIREAI
                     EARRDIAVGVSGIIGGAPLAGMADRLMPAAGLEVTAAGAASAYQGLLRGWLVDERDRE
                     LAPRIERDLGIRVAVADTIMVDDDAAEAVARAALRLLGT"
     gene            3197..4321
                     /gene="cofE"
                     /locus_tag="VIX62_08935"
     CDS             3197..4321
                     /gene="cofE"
                     /locus_tag="VIX62_08935"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY5860393.1"
                     /translation="MSTGPALEIIGVEGIPEVVPGDDLVAIVTPMLLAAGAQDDDVVV
                     ITSKIVSKAEGRLVSGDDRRAAVEAETERVVARRGDLVIAETRHGFVCANAGVDASNL
                     DTGTLALLPLDPDATAERIRHGLGETLGARFGVVITDTFGRAWRTGVVNVAIGCAGVP
                     STVDLRGRPDDRGRPLEATVVALADEIAAASGLVMGKDARVPVALVRGVRRLDGTDGP
                     ATDLIRPTDEDLFRTSTPEAIRSLSTGDAASGSVPIGRELLIEAIGACATPWPPGDPR
                     RGCTVVEDPDEVATMLRALAGRDADGSTLIVPWLEASDDQTLEPGWGFAAGAYVTALA
                     TALAGAGVTVRWFDREEARSLLRVEDGRVPLGVLACGGGG"
     gene            4426..5250
                     /locus_tag="VIX62_08940"
     CDS             4426..5250
                     /locus_tag="VIX62_08940"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY5860394.1"
                     /translation="MTDRAGGDGSRRPTKAERKDQARIEREQIQRQMAARKRNRNIGI
                     AVIALAVVAVLVVVLVVKPGSDPTSPGTADSPADLLAQAPDAKGDAGCDDVQTIGFYD
                     GVNDPESPQYTDQAHIGAPGSAFPEMPPLTSYPSQPPVSGPHANIPPGPLPAGFYDDP
                     PDLARVLHSLEHGASVVWYSPTATDQQLQPLRDFYDRDDPAVGQDRVIVAPYDYEGDG
                     GQLPSGVMMSLGAWHRQQNCSQISLPVAFDFTSQYSAPPFEGREYAGEAPEAGAAL"
     gene            5269..6063
                     /locus_tag="VIX62_08945"
     CDS             5269..6063
                     /locus_tag="VIX62_08945"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY5860395.1"
                     /translation="MANRKKKRQRRPSGPATTAPKTSVEDPAEAKQAPSRGGANMARR
                     ERKEQARAARETERKRAARHAAFRRAAVFSVIGLAAVGVLFWLQRAASPTDLSAEAQT
                     AASAANCSEVITPLGNAPAGEHVPQGTPITYDQRPPTSGQHYQEVVAAQDPAILTEPL
                     DEPASVHFLEHAGIIAYYRDSGDAALPDDVVSAVGEVATAQRNMLMAPFPDLPDGTSL
                     ALTAWNKELTCPGSVTAAQARTIANGFADAFVCTSNAPEPNASGAC"
     gene            complement(6077..7882)
                     /locus_tag="VIX62_08950"
     CDS             complement(6077..7882)
                     /locus_tag="VIX62_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY5860396.1"
                     /translation="MRRTSLLTAVFSLVLAVLGPAAWAAGEGTVPGGVSQDPFTGAPP
                     ATNGGAERGGGFTFFGSGFGHGLGMSQWGSYGLALEGWDHARILKNFYRGTRVVKDQH
                     PVAKLRVGITYDRTLIHLGARSGPVRLWIGKPKGNFVAKIPGGKTWTVRSSGKGYAIR
                     DHTGDLVGGKVWGGRANDLFATYADTGSRVFVKEADAVSGVGYAYNRGFLEFNLYRGG
                     GSWQERVIVPIAMEQYLLGIGEMPSSWPAQALETQAVAARTFATYTARNYGLRAYCKC
                     DITDGANDQVYIGYNKEGGTDGKRWVHAVNATTRQIVTTQQGKVIQAFFAASDGGHSD
                     AVEDVWHSGNPAYKVSYLKAECDPGEYTAANPWTDWKRQLTAGELTSRLSPYTGGIGT
                     VTGFPKIKRGRGGRVITAVVKGTSGKASVSGSELKSAVSAWDSRLWVNKNKNIVGTIR
                     AKYDSLMCKPGLPTSSVVTMAGGARQKFQVGGIYRNSAAGVSVWLRGAIYKEYVGAGG
                     ATGQLGLPSTQVKPVPGTPGERILFKGGRIYAKGGAGTHALWGKVLGEYLDRGAATGS
                     LGFPTSRVHSAGGGTTATFERGTITCPDGGGCSVS"
     gene            complement(7995..9005)
                     /locus_tag="VIX62_08955"
     CDS             complement(7995..9005)
                     /locus_tag="VIX62_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY5860397.1"
                     /translation="MKAVVLVGGEGTRLRPLTETMPKPLLPLMDRRSLDHVLDHLARH
                     GVHEVVLSSPYLESTFHPFIESRGSEPRITWITETEPLGTGGAIVNALDHLDDEPFFA
                     LNGDILTDLDLTAMRSFHADHAAAVTIAIHHVEDARAFGLVATEPDGRVLAFREKPED
                     LIPGDINAGTYLLDPKVLRDRTPGEASSIERDIFPAVIEAGHPVFGFLAEAYWLDLGT
                     PEKYLQAHFDMFEGKVHDVSYPAPWIADGAAVDLRAHLGRWVAVGEGASIGPEAQVDD
                     SVLHADVAIAAGARIAGSIIAKAATVGEGATVIDSVIGEGASVPAGLTLEAARIAAGA
                     IA"
     gene            complement(9002..9874)
                     /locus_tag="VIX62_08960"
     CDS             complement(9002..9874)
                     /locus_tag="VIX62_08960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5860398.1"
                     /translation="MTTDPGAAGLAVVIVNFNTGDYLERCLASLEAHRGDIEIDVLVI
                     DNASHDGSHTRAVAAHPWARLIENPTNVFLSPAWNQGIRETKAPWVLLLNPDIEWWAG
                     TLADYVAVAKAHPDAGIVGPLVRNSDGTIYESGRPFPDVVDAAGHAFLGTVKPGNRFT
                     KRYHLDGWDRRSEREVDWVSGCCMLVPRTAFDRVGMLDEGFPLFGEELDLATRMRDAG
                     LVSLYTPAVEIVHEIGVSRSRSRSMLLMHSNSIYRYYRLHRAGGWRRSTLPLAWATLR
                     VRAELEALRGKVSR"
     gene            complement(9871..10242)
                     /locus_tag="VIX62_08965"
     CDS             complement(9871..10242)
                     /locus_tag="VIX62_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860399.1"
                     /translation="MLIGLILLSVALAALAQLTLKHGMNEVTANSGELQLNAHSLKDV
                     LTTWAVWGGLFLFGLSAFVWLAVLSRASLSFAYPFASLSYVLILLADKFILQEQIPTL
                     RWAGVFFIMTGIVLVAQTPHS"
     gene            complement(10335..>10696)
                     /locus_tag="VIX62_08970"
     CDS             complement(10335..>10696)
                     /locus_tag="VIX62_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HEY5860400.1"
                     /translation="TVWTGAELTISVDVEATSPVAGLDASFAIVSAATNHVVIDGRTS
                     TMGLDVGAVDGKKRIRFRVPATDWVGGKYFVTVGLESLDGRPYHIQTQRYLLQVPEDA
                     RISKPLDVAAAATVEDL"
BASE COUNT         1630 a         3659 c         3777 g         1630 t
ORIGIN      
        1 caccgcgtgc atccgcagct gctcggccga ggtcgcaccg ggccgagcct tgcccgccag
       61 atagatcggt gcgtgctgat ggacgaacgg cagcggcacg aactccgtga acccgccggt
      121 gtcggcctgg atccgcgcca gccggcggag atggcccacc caatggggcg gcgcatcgac
      181 atggccgaac atgatcgtgc tggagctccg gatgcccaga ccgtgtgcgg tgcggatgat
      241 ctcctcccac gtgtcggcgg ggagcttgcc cttggtgagc gcccagcgca cctcgtcgtc
      301 caagatctct gcggccgtcc ccgggatcgt ccccaggcct cgctgcttcg cctcggcgag
      361 gaactcggcg taggaaagac cgagtttcgt ggacccgttc atgacctcca tggggctgaa
      421 ggcgtggatg tgcatgcccg gcgtacgggc cttcaccgcg tcgagcagat cgaagtagaa
      481 cgagcccggc aggtcggggt ggatcccgcc ttgcatgcac acctcggtgg caccgagctc
      541 ccacgcttcc tcggcgcgat cggcgacctc ggcgagggtc agcgtgtagg actcggcatc
      601 catctcgcgt tgcgcgaacg cgcagaaccg gcaccccacg tagcagacgt tcgtgaagtt
      661 gatgttccgg ttgaccacgt aggtcacgtc gtcgccgacg gcctcggcac ggagtcggtc
      721 ggcgacgcga catagcgcat cgagcgccgc tccttctgct tggaacagcg ccaacgcatg
      781 ttcgtcggag atcggtcgat gggcttcggc ggcggctagt gcggccacga tgtcgcgatc
      841 gatccgctcg ggatggatct cacgcttcga ccacgcgcgg gtggtttcgg gggtatcggc
      901 gtcgccgtag acctcatccg cgtccccacg gaggcccgtt ccgtcggcct tggcgaaggt
      961 gagcgcgatc gtccggggct tccactggac gtcgggatcc tgccacgcga tcggttccgg
     1021 atgccgccct tcgacggccc ggccatcggg cccgaggagg gccatcacgg gagcccgcat
     1081 cttgccggcg aggtagggat cgggcttggc ggcgaacgcc ggatagatcg tgaggcgctc
     1141 acggagctgt ttgccccggg aggcggtggt ggccgccagg gcctcgatcg agggccaggg
     1201 cttctccgga ttgacgtggt cgggggtgag gggcgagacg ccgccccaat cgtcgatccc
     1261 ggcgtcgagc aggcggacct gctggtcagg atcgctgagg ttgggcggtg cctgcaggtg
     1321 catgcgcggc ccgagcacca cgcgcgcggt ggcgacggcc gccaagaagg cctcgtcatc
     1381 cggctcgggc gccgcctgca tcgcggtccc cggcttggct cggaagttct ggacgatgac
     1441 ctcttggatg tggcggtacc gccggtgcgc gtcgcggatc gcgaagagcg actcggctcg
     1501 ctcgcgatcg gtctcgccga tgcccaccag gatgccggtg gtgaacggta cggcgagcct
     1561 ccccgcgtct tcgatcgtgc gcagccgcac cgccggcacc ttgtccggcg acccgaagtg
     1621 gggaccccct cgctcggcca ggcgcatcga gctcgtctcg agcatcatgc ccatcgaggc
     1681 actcacctgc ttcaggcggg cgatctcctc gtacgacatc acgccggggt tgaggtgggg
     1741 cagcaggccg gtctcctcga tcacccggat cgcgaccgcg cgcacgtagt ccagcgtgga
     1801 tgcgtatccg cgcgcttcga gccactcgcg cgcgacgtcg tagcgttcct ccggccggtc
     1861 gcccaaggtg aacagcgctt ccttgcagcc catccggcgg ccggcgtcgg cgatcgagac
     1921 cgcctcttcc ggtgtgagga agggtgtatc gagcttggcg ggcggcttcg cgaacgtgca
     1981 gtagtggcag tggtcgcggc acagcatggt gagcgggatg aacaccttgc gcgaataggt
     2041 gacgacctca ccgtgctcca ggtcgcgcag gcgggccgcg agccccatga ggcgatcgag
     2101 ttcctcgccc cgggcggcca tcaacgacgt ggcctcatcg agggtgagcg ccttgccggc
     2161 atcggctcgg gcgagcgccc ggcggacctg ccgaggggcg tcggagccgg tcacctcggg
     2221 catgttcgcg agcctactac gctgtgttcg gtatgaaggt cgtggcgctg gcgggtggca
     2281 tcggaggcgg gaagttcctg cgtgggttga tccgcgccgt gccaccgaac gacgtcatcg
     2341 ccgtggtcaa caccggtgac gacctgtgga tgcacggcct gcggatctgt ccagacctcg
     2401 attcggtgac ctactggctc ggcgacgcgt tcgaccgcga gcgcggctgg ggacgccggg
     2461 acgagacctt ccgggccacg gaggagctgc ggcggttcga ccccgaagcg gcgtggttcg
     2521 ggcttggcga tctcgatctc gcgacccatc tgtaccgaac ccgtttgctg gcggacggtc
     2581 gctcgctgag cgagatcacg gggctggtcg ccgctcggtt cggcatcgcc gctcggatcc
     2641 tgccgatgag cgacgacgac gtcaccacgc gcgtggacgt cgtctcgttc ggccaggaga
     2701 tggatctgca cttccaggag tactgggtgc aacgcggcgg gcgggacgac gtcaagggtg
     2761 tgcggttcca gggtctggac gctgcccggc ccgcacccgg ggtgttggag gcgctggccg
     2821 aggccgacgc gatcgtcttc gcgccgtcga accccgtggt ctcgatcggg ccgatcctgg
     2881 gggtcccggg gatccgcgag gcgatcgaag cccgacgcga catcgcggtg ggcgtgagcg
     2941 ggatcatcgg cggggcaccg ctggcgggga tggccgaccg gctgatgccg gctgcagggc
     3001 tggaggtgac ggcggcggga gccgcgagcg cctaccaggg gctgttgcgc ggctggttgg
     3061 tcgatgaacg cgaccgtgag ctcgctccga ggatcgaacg cgacctgggg atccgagtgg
     3121 cggtcgccga cacgatcatg gtggacgacg acgccgccga ggccgtggcc cgggcggcac
     3181 tgaggttgct ggggacgtga gcaccggacc ggcactggag atcatcggag tcgaagggat
     3241 ccctgaggtc gtaccgggcg acgacctcgt cgccatcgtg acgccgatgc tcctggcggc
     3301 gggcgcccaa gacgacgatg tcgtggtcat cacgtcgaag atcgtgtcca aggccgaggg
     3361 acggttggtg tccggggacg atcgaagggc ggccgtggag gccgagaccg agcgggtggt
     3421 ggcccggcgg ggcgacctgg tgatcgccga gacccggcac gggttcgtgt gcgcgaacgc
     3481 cggcgtcgat gcctcgaacc tggacacggg cacgctggcg ctgctgccgc tcgaccccga
     3541 cgccaccgcc gagcgcatcc gacacggact gggggagacg ctcggcgccc ggttcggggt
     3601 ggtcatcacg gacacgttcg gtcgtgcctg gagaacgggc gtggtcaacg tcgcgatcgg
     3661 ctgcgccggt gtgccctcga ccgtggacct gcgcggccgg ccggacgacc ggggccgccc
     3721 cctggaagcg accgtggtgg cgctcgccga tgagatcgcg gccgcgagcg gcctggtgat
     3781 gggcaaggac gcccgggtgc ccgtggcgct cgtccgaggt gtgcgccgcc tcgacgggac
     3841 cgacggcccg gccaccgatc tgatccggcc gaccgacgag gatctgttcc ggacctcgac
     3901 ccccgaagcg atccgctcct tgtcgaccgg cgacgcggcg tccggctcgg tcccgatcgg
     3961 gcgcgagctc ctgatcgagg ccatcggcgc ctgtgcgacc ccatggcccc ccggcgaccc
     4021 ccggcgcggc tgcaccgtgg tcgaggaccc cgatgaggtc gccacgatgc tgcgcgcgct
     4081 ggcggggagg gacgccgacg ggtccacgct catcgtcccg tggctcgaag cgtccgatga
     4141 ccagacgctc gagcccggat gggggttcgc cgcgggggcg tacgtcaccg cgctggcgac
     4201 ggcgttggcc ggcgccggcg tcacggttcg ctggttcgac cgggaggaag cgcgatcgtt
     4261 gctccgagtc gaggacgggc gggtgccgct cggcgtgctg gcgtgcggtg gtggtgggta
     4321 gcgggcccgg atcgcgcgac aggatcaccg catctgcctg ggcctcgcgg accgttccgt
     4381 tgggtaccct gagcgttccg aggacgacga ggagatcgaa cgcccgtgac cgatcgagca
     4441 ggtggcgacg gcagccgacg ccccacgaaa gccgagcgca aggatcaggc acgcatcgag
     4501 cgtgaacaga tccaacgcca gatggccgcc cgaaagcgca accggaacat cggcatcgcg
     4561 gtgatcgcgc tcgcggtcgt ggccgtgctg gtggtggtgc tggtggtcaa gcccggaagc
     4621 gatcccacat cgcccggcac cgccgactcc cccgccgacc tgttggccca agcccccgac
     4681 gcgaagggcg acgccggctg cgacgacgtg cagacgatcg gcttctacga cggcgtcaac
     4741 gacccggaga gcccgcagta caccgatcag gcgcacatcg gggccccggg cagcgcgttc
     4801 ccggagatgc cgcccctcac cagttacccg agccagccgc ccgtctcagg gccgcacgcg
     4861 aacatcccgc ccggtccgct tcccgcgggc ttctacgacg acccgccgga tctcgcgcgc
     4921 gtgctccact cgctcgagca cggcgcgtcg gtggtctggt acagcccaac ggccaccgac
     4981 caacagctgc agccgctgcg ggacttctac gaccgcgacg atccggccgt gggacaggac
     5041 cgtgtgatcg tggcgcccta cgactacgag ggcgacggag gccagttgcc cagcggcgtg
     5101 atgatgtcgc tcggcgcctg gcaccgtcag cagaactgtt cccagatcag cctcccggtg
     5161 gccttcgact tcacgagcca gtactcggcc ccgcccttcg agggccgcga gtacgcaggc
     5221 gaagctcccg aagccggcgc cgccctctag ttcgcggaag acgacaccat ggccaaccgg
     5281 aagaagaagc ggcagcgccg accgagcggc ccggcgacca cggctcccaa gaccagcgtg
     5341 gaggatcctg ctgaggcgaa gcaggctccg tcgcgaggag gcgccaacat ggcgcggcgc
     5401 gagcgcaagg agcaggcgcg cgcagcccgc gagaccgaac gcaagcgggc ggcccgccac
     5461 gccgccttcc ggcgagcggc agtcttctcc gtcatcggcc tggcggccgt tggcgtgctg
     5521 ttctggctgc agcgggccgc ttcacccacc gacctgtcgg cggaggcgca aacggccgcc
     5581 agcgcggcga actgcagcga ggtcatcacg ccactgggca acgcgccggc aggcgaacac
     5641 gtgccacagg gcacgccgat cacttacgac cagcgaccgc ccacgtccgg tcagcactat
     5701 caggaagtgg tggccgccca ggatccggcg atcctcaccg agccgctcga cgagccggcc
     5761 tcggtgcact tcctggagca cgctggcatc atcgcctatt accgggactc gggcgatgcg
     5821 gcgctccccg acgacgtggt gtcggcggtg ggcgaggtcg ccacggcgca acggaacatg
     5881 ttgatggcgc cgttccccga cctgcccgac ggcacatcgc tcgccctcac ggcatggaac
     5941 aaggagctca cctgcccggg cagcgtgacg gctgcgcaag cgcgaacgat cgcgaacggg
     6001 ttcgccgatg cgttcgtgtg cacgagcaac gctccggaac cgaacgccag cggcgcgtgc
     6061 tgatcgcggc gggccgctac gacaccgagc agccgccgcc gtctgggcag gtgatggtgc
     6121 cgcgctcgaa cgtcgcggtg gtgccgccgc cggcgctgtg caccctcgag gtggggaagc
     6181 ccaacgaccc ggtggccgcg ccgcggtcca ggtactcgcc cagcaccttc ccccacagag
     6241 cgtgggtccc ggcgccgccc ttggcgtaga tgcgtccccc cttgaacagg atccgctcgc
     6301 ccggggtgcc gggcaccggt ttcacctgcg tcgaaggcag gcccagttgg ccggtggcgc
     6361 cgcccgcgcc gacgtactcc ttgtagatcg cgccgcgaag ccagaccgag accccggcgg
     6421 cgctgttgcg gtagatcccg cccacctgga acttctgtcg tgcacccccg gccatcgtga
     6481 cgacggacga cgtggggagc cccggcttac acatcagcga gtcgtacttc gcgcggatcg
     6541 tgcccacgat gttcttgttc ttgttgaccc acagccggct gtcccaggcc gagacggcgc
     6601 tcttgagctc cgagcccgac accgacgcct tgccggacgt gcccttgacc accgcggtga
     6661 tcacccgtcc gccccgtcct cgcttgatct tggggaagcc ggtcaccgtg ccgatgccgc
     6721 ccgtgtacgg ggagagccgg ctcgtgagtt cgccggcggt gagctgccgc ttccagtcgg
     6781 tccacgggtt ggccgccgtg tattcgccgg gatcgcattc ggccttcagg taggagacct
     6841 tgtaggcggg attcccgctg tgccagacgt cctcgacggc atcggagtgt ccaccgtcgg
     6901 acgccgcgaa gaacgcctgg atcaccttgc cctgctgcgt ggtgacgatc tgccgggtgg
     6961 tggcgttgac cgcgtgcacc cagcgcttgc cgtcggttcc gccttccttg ttgtagccga
     7021 tgtagacctg gtcgttggcg ccatcggtga tgtcgcactt gcagtaggcg cgcagcccgt
     7081 agttccgcgc ggtgtaggtt gcgaacgtcc gagcggccac ggcctgggtc tccaacgcct
     7141 gcgccggcca cgaactcggc atctcgccga tccccaggag gtactgctcc atcgcgatcg
     7201 gcacgatcac ccgttcctgc catgagccgc cgccccggta gaggttgaac tcgaggaacc
     7261 cccggttgta ggcgtaaccg acaccgctca cggcatcggc ttccttcacg aagacgcggg
     7321 agcccgtgtc ggcgtaggtg gcgaacaggt cgttggccct accgccccag accttgccac
     7381 ccaccaggtc gccggtgtgg tcgcggatcg cgtagccctt gcccgaggaa cgaacggtcc
     7441 aggtcttgcc gccggggatc ttcgccacga agttcccctt gggcttgccg atccagaggc
     7501 gcaccgggcc cgagcgggcg cccaggtgga tcagggtgcg gtcgtaggtg atccccacgc
     7561 gcagcttggc caccgggtgc tgatccttga ccaccctggt gccgcggtag aagttcttga
     7621 ggatccgagc gtggtcccag ccctccaggg ccaggccgta ggacccccac tgcgacatgc
     7681 ccagcccgtg cccgaacccg gatccgaaga aggtgaaccc gccgccacgc tcggcgcccc
     7741 cgttcgtcgc gggtggcgcc cccgtgaagg ggtcctgact gacgccgccc ggcacggtcc
     7801 cttcccctgc agcccatgcc gcaggtccga gcacggccag cacgagcgag aacaccgcgg
     7861 tgagcaggct cgttcgtcgc atcctggatg accccccggt caggttcggc acgttcttac
     7921 cttggcacat cgacgtagga catccactga cgcatcggcg ccgttcggcc acctattgag
     7981 gcgacgaggt gggcctaggc gatcgccccg gccgcgatgc gggccgcctc cagcgtgagg
     8041 ccggccggca ccgaggcgcc ttcgccgatg accgaatcga tcacggtggc gccttctccc
     8101 accgtggcgg ccttcgcgat gatcgaaccg gcgatgcgcg cgccggccgc gatcgccacg
     8161 tcggcgtgca gcaccgaatc gtcgacctgt gcctcgggcc cgatcgaggc gccctccccc
     8221 accgccaccc accggccgag atgggcacgc agatcgaccg cggcgccgtc cgcgatccac
     8281 ggagcggggt aggacacgtc gtgcaccttg ccctcgaaca tgtcgaagtg cgcctgcagg
     8341 tacttctccg gtgtccccag gtcgagccag taggcctcgg cgaggaaccc gaacacggga
     8401 tggcccgcct cgatgaccgc cgggaagatg tcccgctcga tcgacgatgc ctcgccgggc
     8461 gtgcggtcgc ggagcacctt cgggtccagc aggtaggtgc cggcgttgat gtcgccgggg
     8521 atcaggtcct cgggcttctc ccggaacgcc agcacgcgcc cgtcgggctc ggtagccacc
     8581 aggccgaacg cccgcgcatc ctccacgtgg tgaatggcga tcgtgacggc ggcagcatga
     8641 tctgcgtgga acgaacgcat cgcggtgagg tcgagatcgg tgaggatgtc cccgttcagg
     8701 gcgaagaacg gctcgtcgtc gagatggtca agggcattga cgatcgctcc cccggtgccc
     8761 aggggttcgg tctcggtgat ccaggtgatg cgaggttctg agccccggga ctcgatgaac
     8821 ggatggaacg tggactccag gtacggcgag gagagcacca cctcgtgcac gccatggcga
     8881 gccaggtggt cgagcacgtg gtcgagcgat cgccggtcca tcaagggcag gagcggcttg
     8941 ggcatcgtct cggtgagcgg acgcaggcgc gtgccctcgc cgcccaccag caccacggcc
     9001 ttcatcggga caccttccca cggagcgcct cgagttcggc ccgcacgcgg agcgttgccc
     9061 aggcgagcgg gagcgtggag cgtcgccatc ccccggcgcg atggagccgg tagtagcggt
     9121 agatgctgtt cgagtgcatc agcagcatcg accgagaccg gctccgggag acgccgatct
     9181 cgtgcacgat ctcgaccgcc ggggtgtaca gcgacaccaa ccccgcatcg cgcatccggg
     9241 tcgccagatc gagttcctcg ccgaagagcg ggaacccctc gtcgagcatg cccacgcgat
     9301 cgaaggcggt gcgcggcacc agcatgcaac aaccggagac ccagtccacc tcacgctccg
     9361 aacgccggtc ccacccgtcg aggtggtaac gcttcgtgaa gcggttgccg ggcttgacgg
     9421 ttcccaggaa ggcatggccg gccgcgtcga ccacgtcggg gaacgggcgt ccgctctcgt
     9481 agatcgtgcc gtcggagttg cgcaccagcg ggcccacgat gccggcgtcg ggatgggcct
     9541 tcgcgacggc gacgtagtcg gcgagcgtgc cagcccacca ttcgatgtcg gggttgagca
     9601 ggagcaccca cggcgccttc gtctcccgga tgccctggtt ccacgcaggc gagaggaaca
     9661 cgttcgtcgg gttctcgatc agacgcgccc acggatgggc ggcgaccgcc cgcgtgtggg
     9721 aaccgtcgtg cgaggcgttg tcgatcacca ggacgtcgat ctcgatgtcg ccacggtgcg
     9781 cttcgaggga cgcgaggcag cgctccagat agtccccggt gttgaagttc acgatgacga
     9841 cggccagccc ggccgccccg ggatcggtgg tcatgagtgc ggcgtctgcg ccaccagcac
     9901 gatgccggtc atgatgaaga acacgccggc ccatcgcagc gtcgggatct gctcctgcag
     9961 gatgaacttg tcggccagca ggatcagcac gtacgacagc gaggcgaacg gatacgcgaa
    10021 cgacagcgag gcgcgggaca ggaccgccag ccacacgaat gccgacagcc cgaacaggaa
    10081 cagcccgccc cacaccgccc acgtggtcag cacgtccttc agcgagtgcg cgttgagctg
    10141 cagctcaccg ctgttggcgg tcacctcgtt catgccgtgc ttgagggtga gctgggccag
    10201 ggcggccagc gccaccgaca gcaggatcag accgatcaac atcgtgcggt tcttccttcc
    10261 tcttccttga tcgtgatggc cttcaacgag atgcgcctcg tgggcctcgg gatcgagcac
    10321 gggggggttc gggttcacag gtcctccacg gtggcggcgg ccgccacgtc gagcggcttc
    10381 gagatgcgag catcctccgg cacctgcagg aggtagcgct gggtctggat gtggtacggg
    10441 cggccgtcga gcgactcgag gccgaccgtg acgaagtact tgccgcccac ccagtcggtg
    10501 gccggcacac ggaaccggat gcgcttcttg ccgtcgaccg cgccgacgtc gaggcccatc
    10561 gtcgaggttc ggccgtcgat cacgacgtgg ttggtggccg ccgagacgat cgcgaacgag
    10621 gcgtcgagcc ccgcgaccgg tgacgtcgct tccacgtcca cgctgatcgt gagctcggcg
    10681 ccggtccata ccgtgc
//
LOCUS       DATDBZ010000375         5502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1765111, whole genome shotgun sequence.
ACCESSION   DATDBZ010000375 DATDBZ010000000
VERSION     DATDBZ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765111"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1389)
                     /locus_tag="VIX62_08975"
     CDS             complement(40..1389)
                     /locus_tag="VIX62_08975"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY5860401.1"
                     /translation="MPELLGADGFAPVADAALELSDVDGVEVLFLHEWGGLTRFAKSE
                     IHQSTNREDTGLRVRVVSKGRVGVAATNECTPEGARKAAQSAKEMAEIVGPDPLWPGL
                     GPVAQTPQVDRFFEGTANASPETRAEAVADLIGTCAPGFTAAGAYETMAMEVGLANTE
                     GQRCWAPSTQASVTTVISGSEGGSGFAETFSGSVETIDAGAIGQRANDKALGSRSPKA
                     LDAGVYPVVLEPAAVATLIGFLAWVGFGGKGYLEDRSCFSGKKDQQVAAPTVSIWDDG
                     ADPRTLGAPFDFEGVPKRRVDLIRDGVFLDAVYDLRTGKEAGVPSTGHGLPSPNPEGP
                     FPLNLFMDTGDATVEDMVKGTERGVLVTRFHYANIVNPIESSITGMTRDGTFLIERGE
                     VVGPVRNFRFTQSILGALSTTSMVGRQAELASEFFFSASRVPALKVEEFNFSGVSDH"
     gene            complement(1389..2828)
                     /locus_tag="VIX62_08980"
     CDS             complement(1389..2828)
                     /locus_tag="VIX62_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY5860402.1"
                     /translation="MLDVAKMAVEAATAAGADLADARAGTDETESLTVRNQEMAGIDR
                     SISTGVGIRVLVGGRWGFASTSRLEPEEITRTAVLAVEIAKAAQRLPGEPVTLSPVEP
                     AVASWQGPMQEDPFTVPLEEKVALLMEATRRMQSVEGLTFAEAGLDFFRRSTWFASSD
                     GAAIEQVVLHSGGGTEAIAVGDGDMQRRSYPNSFRGHIKAAGYEHIRTLGLIEEAERI
                     GQEAVDLLNAPECPSEVTTLVLDSGQMELQIHESIGHPIELDRVLGMEEAYAGGSWLT
                     PGDRGKVRYASDLVSITADATLAGGLGTFGFDDEGVPAQSTPIIVDGMFENFLTSRET
                     APVVGQTSNATSRADGWSHLPLIRMTNISIEPREGTLAEIIGDTDDGIFMSTNTSWSI
                     DDKRINFQFGCEMAWRIEHGKLTTLYKNPNYTGLAPEFWGSCDAVGGREDWVVWGTPN
                     CGKGQPGQVGRVGHGASPARFRNVQVGVR"
     gene            2960..3265
                     /locus_tag="VIX62_08985"
     CDS             2960..3265
                     /locus_tag="VIX62_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HEY5860403.1"
                     /translation="MDTKTEEEVGDMTVPRIGYTNEQAAAVADEIGLNLAEVPFDIDQ
                     FRRGMEVELEHGVRDPVTNVTNDDPLITGKIAWAHLREFPDYYDRLAVMEAEAELQR"
     gene            3262..3747
                     /locus_tag="VIX62_08990"
     CDS             3262..3747
                     /locus_tag="VIX62_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEY5860404.1"
                     /translation="MILVAYASKHGATEGIAHAIAKRLDYRDLDAQAHTVHELEDVGR
                     PEAVVLGSAVYAGSWMKDAVRFAEAHAEALADVPVWLFSSGPLGEEVKDEEEQPRQLP
                     ELRELLHPREHRVFFGALDREKLSFSERMIVKAVKAPDGDFRDWDAINGWADEIADEL
                     R"
     gene            3823..4197
                     /locus_tag="VIX62_08995"
     CDS             3823..4197
                     /locus_tag="VIX62_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5860405.1"
                     /translation="MPVERAADHPTFELGGNTITSLAAPARGADEAALFRIDVPGGGG
                     LPAHHHDHLDVFTVERGGGTFHIGEEIFELTAGDSVVVPIGEVHYLVAGPQGASMVVT
                     MLAGTTLVRADDGVELVPPWVS"
     gene            4200..5393
                     /locus_tag="VIX62_09000"
     CDS             4200..5393
                     /locus_tag="VIX62_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5860406.1"
                     /translation="MAGFYVADVTLFDGAKVRRRHGVLVQEGRIAWVGPHTRAPREAT
                     AARAVDGAGRTLTPGLIDCHVHLCFDGIGDFAADAARMHASPMLAPIKAVRNAAKHLA
                     RGVTTVRDLGGIGTCELASAIEDGVVPGPRVVAAGRALTVTGGHGHNVAFARQVDGAD
                     AVRKAVREEIRAGAAAIKVVATGGVLTPGIGATFTAFTPEELDAAVDEAHKWNRGVAA
                     HAIGAEGVTQSVTAGVDSVEHCVQLTTASARRMAERGTFRGPTLCAAFGMIENPEGTP
                     AYALEKISSVIDDAERSNGVALRAKVRHVCSTDAGTPFNEHGNAPLELQRMVAWGMSP
                     LDAIVAGTANGAELLRVPDIGSVAEGMRADLTLYDANPAEDIEALDAPRTVWKDGLVV
                     AGRRS"
BASE COUNT          820 a         1962 c         1878 g          842 t
ORIGIN      
        1 catgtcgcca gcgtacgccc tgcgtcggct gtgtggggcc tagtggtcgc tgacgccgga
       61 gaaattgaac tcctccacct tgagcgcggg cacgcgtgag gcgctgaaga agaactcgct
      121 cgccagctcg gcctgacggc ccaccatgga cgtggtggac agcgcgccga ggatcgattg
      181 cgtgaaccgg aaattccgca ccggcccgac gacctcgccg cgctcgatca ggaacgtacc
      241 gtcgcgggtc atgccggtga tggaactctc gatcgggttc acgatgttgg cgtagtggaa
      301 ccgcgtcacc agcacgccgc gttccgtgcc cttcaccatg tcctcgacgg tggcgtcccc
      361 ggtgtccatg aacaggttga gcgggaacgg cccctcgggg ttgggcgagg ggaggccgtg
      421 tccggtcgag gggacgcctg cctccttgcc ggtccgcagg tcgtagaccg cgtccaggaa
      481 caccccgtcg cggatcaggt cgacgcgccg cttcgggacg ccctcgaagt cgaacggcgc
      541 tcccagcgtg cgcgggtcgg cgccgtcgtc ccagatcgag accgtgggtg ccgccacctg
      601 ctggtccttc ttgccgctga agcaggatcg gtcttccagg tagcccttgc cgccgaaccc
      661 cacccaggcc aggaatccga tcagcgtggc caccgccgcc ggctccagca ccacggggta
      721 gacgcccgca tcgagggcct tcggcgagcg tgatcccagc gccttgtcgt tggcgcgctg
      781 gccgatcgcg ccggcgtcga tcgtctcgac cgacccggag aaggtctcgg cgaagccgct
      841 gccgccctcg gagcccgata tcaccgtggt gaccgaggcc tgcgtggagg gcgcccagca
      901 tcgctggcct tcggtgttgg cgagccccac ctccatcgcc atggtttcgt aggcgccggc
      961 ggccgtgaac ccgggtgcgc acgtgccgat cagatcggcg accgcttcgg cgcgcgtctc
     1021 gggcgaggcg ttcgccgttc cttcgaagaa ccggtccacc tgcggcgtct gcgcgaccgg
     1081 ccccaggccc ggccacaacg gatcgggccc cacgatctcg gccatctcct tggcgctctg
     1141 cgcggctttg cgcgctccct cgggcgtgca ctcgttcgtc gccgccacgc cgacgcgacc
     1201 cttcgacacc acgcggaccc gcaggccggt gtcctcccgg ttcgtgctct ggtggatctc
     1261 gctcttggcg aacctcgtca ggccacccca ttcatgcagg aacagcacct cgacgccgtc
     1321 gacgtccgac agctccagcg cggcgtcggc gaccggcgcg aacccgtcgg cacccagcag
     1381 ctcgggcatc agcgcacccc cacctggacg ttgcggaacc gcgccggcga ggcgccgtgt
     1441 cccacccggc ccacctgccc gggctggccc ttgccgcagt tgggcgtgcc ccacaccacc
     1501 cagtcctcgc gcccgcccac cgcatcgcat gaaccccaga actccggtgc gagtccggtg
     1561 tagttcgggt tcttgtagag cgtcgtcagt ttgccgtgct cgatgcgcca cgccatctcg
     1621 cacccgaact ggaagttgat gcgcttgtcg tcgatcgacc acgaggtgtt cgtggacatg
     1681 aagatgccgt cgtcggtgtc gccgatgatc tcggccagcg tgccttcccg cggttcgatc
     1741 gagatgttcg tcatgcggat caggggcagg tggctccacc catcggcgcg gctcgtggcg
     1801 ttggaggtct gtcccaccac cggcgccgtc tcccgggagg tcaggaagtt ctcgaacatt
     1861 ccgtcgacga tgatcggcgt ggactgcgcc ggcacgccct cgtcgtcgaa cccgaacgtg
     1921 cccagcccac cggcgagcgt cgcgtcggcg gtgatcgaga ccagatcgct cgcatagcga
     1981 accttccccc ggtcgcccgg ggtgagccag gacccgcccg cgtaggcctc ctccatgccg
     2041 agcacgcggt cgagctcgat ggggtggccg atcgactcgt ggatctggag ttccatctgc
     2101 ccgctgtcca gcaccagggt ggtgacctca ctgggacact cgggcgcgtt cagcaggtcg
     2161 acggcttcct ggccgatgcg ctcggcctcc tcgatcagcc cgagcgtgcg gatgtgttcg
     2221 tagcccgccg ccttgatgtg accacggaac gagttgggat agctgcggcg ctgcatgtca
     2281 ccgtcgccca ccgcgatggc ctcggtgccg ccgcccgagt gcagcaccac ctgttcgatc
     2341 gcggccccgt cgctcgaggc gaaccaggtc gagcggcgga agaagtccag tcccgcctcg
     2401 gcgaacgtga ggccctcgac cgactgcatc cgccgcgtgg cttccatcaa cagcgccacc
     2461 ttctcctcca gtggcaccgt gaacgggtcc tcctgcatcg ggccctgcca gctggcgacc
     2521 gcgggctcca cgggcgacag cgtcacgggc tcaccgggga gccgctgcgc cgccttcgcg
     2581 atctcgacgg cgaggactgc cgtccgcgtg atctcctcgg gttccaggcg agaggtcgag
     2641 gcgaaccccc accggccgcc caccagcacg cggatcccga ccccggtgga gatcgaacgg
     2701 tcgatgccgg ccatctcctg gttgcggacg gtgagcgact cggtctcgtc ggtgccggcg
     2761 cgagcatcag cgagatcggc tcctgcggct gtggcggcct cgaccgccat cttcgcgacg
     2821 tccagcaccc agatcctccc tccgcatgcc cgcccgggca cctggtccgc ctgcgctgct
     2881 gcgctcgagg ggccactggt cccggcggaa ccggggcatc ctacccggtg accgcggcat
     2941 ccatgggcct acggtgagca tggatacgaa gaccgaggaa gaggtgggcg acatgacggt
     3001 tccgaggatc ggctacacga acgagcaggc ggccgcggtg gccgatgaga tcggattgaa
     3061 cctcgccgag gtgccgttcg atatcgacca gttccggcgg ggcatggagg tggaactcga
     3121 gcacggcgtc cgcgatccgg tgacgaacgt cacgaacgac gatccgctga tcaccggcaa
     3181 gatcgcgtgg gcgcacctgc gggagttccc ggactactac gaccgcctcg ccgtgatgga
     3241 agccgaagcg gagctgcagc gatgatcctg gtggcgtacg ccagcaagca cggagcgacc
     3301 gaaggcatcg cgcacgcgat cgcgaagcgg ctggactacc gcgacctcga cgctcaggcg
     3361 cacacggtcc acgagctcga agacgtggga cggccggagg ccgtcgtgct gggcagcgcc
     3421 gtctacgcgg gttcgtggat gaaggacgcc gtccggttcg ccgaagcaca cgccgaggcg
     3481 ctggccgacg tgccggtgtg gctcttcagc agcggtcctc tcggcgagga ggtcaaggac
     3541 gaggaggaac agcctcgcca gctcccggag cttcgtgagc tgctccatcc ccgcgagcat
     3601 cgtgtgttct tcggcgcgct cgatcgcgag aagctgagct tcagcgaacg catgatcgtg
     3661 aaggcggtga aggcgcccga cggcgacttc cgcgactggg acgcgatcaa cggctgggcc
     3721 gacgagatcg ccgacgaact gcgctgagcc gccggttggt ccctggcggg ctcccggtgg
     3781 gatgatgcgc cccatcacgc atccgagccg aaggagttcc tgatgcccgt cgagcgcgcc
     3841 gccgaccacc ccacgttcga actcggtggc aacacgatca ccagcctcgc cgcccccgcc
     3901 cgtggcgccg acgaggcggc gttgttccgc atcgacgtgc ccggcggtgg cggtctgccg
     3961 gcccaccatc acgaccatct cgacgtcttc acggtggagc ggggaggcgg cacgttccac
     4021 atcggcgagg agatcttcga actcaccgcg ggtgattccg tggtcgtgcc gatcggggag
     4081 gtccactacc tggtggccgg gccccagggc gcatcgatgg tcgtgacgat gctcgccgga
     4141 accacgctcg ttcgcgccga cgacggcgtc gagctcgtcc caccctgggt gagctgatcg
     4201 tggccgggtt ctacgtcgcc gacgtcacgt tgttcgacgg cgccaaggtc cgccgtcgcc
     4261 atggtgtcct cgtgcaggaa ggccgcatcg cgtgggtagg tccgcatacc cgcgcgccgc
     4321 gggaggccac ggccgcccgc gcggtcgacg gcgccggcag gaccctgacg cccggtctga
     4381 tcgattgcca cgtgcacctg tgtttcgacg ggatcggcga cttcgccgct gacgcggcgc
     4441 gcatgcacgc ctcgccgatg ctggcgccga tcaaggcggt ccgcaacgcg gccaagcacc
     4501 tggcccgcgg ggtgaccacc gtgcgcgacc tcggagggat cggaacgtgc gagctcgcct
     4561 ccgcgatcga ggacggggtc gtgccggggc cgcgggtcgt cgcggccggt cgggcactca
     4621 ccgtcaccgg cggtcacggc cacaacgtcg cgttcgcccg ccaggtggac ggcgccgacg
     4681 ccgtacgcaa ggccgtccgc gaggagatcc gtgccggggc cgccgcgatc aaggtggtgg
     4741 ccacgggcgg cgtgctcacc cccgggatcg gcgccacgtt caccgcgttc acgcccgagg
     4801 agcttgacgc cgcggtcgac gaagcccaca agtggaaccg gggcgtcgcc gcgcacgcga
     4861 tcggcgccga gggcgtcacc cagtcggtca ccgccggggt cgattccgtg gagcactgcg
     4921 tgcagctcac gacggcgtcg gcccgccgca tggccgaacg tggcaccttc cgaggaccca
     4981 ccctgtgcgc ggccttcggc atgatcgaga accccgaggg cacgccggcc tatgccttgg
     5041 agaagatcag ctcggtgatc gacgacgccg agcgcagcaa cggcgtggcg ctccgcgcga
     5101 aggttcgcca cgtgtgcagc accgacgcgg gcaccccgtt caacgaacac ggcaacgcgc
     5161 cgctggagct ccagcggatg gtggcctggg gcatgtcgcc gctcgacgcg atagtcgcgg
     5221 gcacggccaa cggtgccgag ctccttcgcg tgcccgacat cggctcggtc gccgaaggca
     5281 tgcgcgccga cctcacgctc tacgacgcca accccgccga agacatcgag gcgctcgatg
     5341 cgccacggac cgtctggaag gacgggctcg tggtggccgg tcgtcgttcc tgagacccag
     5401 catgaccttc cgtgacgaga tcctggcggc gaacgcccga ttcgcctcgc gcttacccgc
     5461 gatcgatcgt gcggcgcgcc ccacgcgggc gatcgcggtg ct
//
LOCUS       DATDBZ010000376         2933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1562820, whole genome shotgun sequence.
ACCESSION   DATDBZ010000376 DATDBZ010000000
VERSION     DATDBZ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562820"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VIX62_09005"
     CDS             complement(<1..1430)
                     /locus_tag="VIX62_09005"
                     /inference="COORDINATES: protein motif:HMM:NF044948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5860407.1"
                     /translation="MTVRGRSFDTYVALVTIAGCSLLVWFVAQPSLEAAASERAFGLL
                     ALLLLVGEVFPISVPRGGEVDELTASTTFAFALMIGFGTPAAVVAQVVASMIADILLH
                     KSLWKVAFNAGQFALSLGAAGAVYHGLGGGMTLNSGSILPFLASAITFFLLNTIITDV
                     AVAMSSGVAMNPYIAKDLVFQAQATLPLLALAPVVVAAVDESLWLIPLAAAPAVALWW
                     GTRLALENARLAQELQRSLEQEQELNRMKDDFVAMVSHELRTPLTSIQGYIKTLRQLS
                     SDLPPDQREAFLEAADRQGDRLRRLIEQLLVVGRLESHVEPLAVTPISIDRLTTNVVD
                     ELRMGAGGHVFDVRIDPNLPRIRSDEGKVHQILSNLVENALKYTPPDTHVAIAADPAV
                     GGIIVSVTDQGQGIPEEHRERIFERFYQVDGSATRRVGGTGLGLYICVKMAETLGGRL
                     WLARSDDHGSVFSLFLPELPPGFEDEG"
     gene            complement(1515..2384)
                     /locus_tag="VIX62_09010"
     CDS             complement(1515..2384)
                     /locus_tag="VIX62_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5860408.1"
                     /translation="MTDEGALVRIDDTALWVVDRGEGYPVIVLHGGPGLDHHEFADYL
                     DPLTERGYRLLLVDLRACGRSEVPPVRSWTLERHAQDVIMLARALRLDRYAVLGHSFG
                     AFVALQNAVDYQGMAAQTIVSSGVASMRWFEAVEPNLLSFEPVSLRRQVADSWAREPE
                     VQTPEEFASLMVDQFPFHFADPVDPRIDDYLARTSDTVYAPDVLRHFATTDAGDIELE
                     DRLDEVASPMLVLAGRHDRTCVPEAAEAIAASVPRSELVVFERSGHMAFVEEQDAYLA
                     AVSGFLDPHRDPS"
     gene            complement(2381..>2933)
                     /gene="npdG"
                     /locus_tag="VIX62_09015"
     CDS             complement(2381..>2933)
                     /gene="npdG"
                     /locus_tag="VIX62_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEY5860409.1"
                     /translation="ETLADTAQVTGTTNEDAAASVDVLMVTVPFAGQADIYRSIAGSV
                     RPGAIVMDATSPLATAVGGRAWQVVRPWHGSAAEQASALLGEGPRVVAAFHTIAAHAL
                     ADLEHPLDSDVLVCGDDDDAKTVIGGLIDDIPDLRWIDCGALSMARITETLTPLLINV
                     NRRYKTKDSGFRIVGREGWGSPP"
BASE COUNT          499 a          989 c         1009 g          436 t
ORIGIN      
        1 ccctcgtcct cgaaccccgg cggcaactcc ggcaggaaga gcgagaacac cgatccgtgg
       61 tcgtcggaac gagcgagcca gagccggccg cccagggtct cggccatctt cacgcagatg
      121 tacagaccca accccgtgcc acccaccctg cgggtggccg atccgtcgac ctggtagaag
      181 cgctcgaaga tccgttcacg atgctcctcc gggatcccct ggccctggtc ggtgacggac
      241 acgatgatcc ctccgacggc ggggtcagcg gcgatcgcta cgtgcgtgtc gggcggcgtg
      301 tacttcaagg cgttctcgac gaggttcgac aggatctggt gcaccttgcc ctcatcggat
      361 cggatccggg gcaggttcgg gtcgatccgg acgtcgaaca catggccccc ggctcccatc
      421 cgcagttcgt ccacgacgtt ggtggtgagt cgatcgatcg agatcggcgt gacggcgagc
      481 ggttccacgt ggctctcgag acgacccacg acgaggagtt gctcgatcag gcggcgaagc
      541 cggtcgccct gccggtcggc cgcttccagg aacgcttccc gctgatcggg gggcaggtcc
      601 gaggacagct gccgcaacgt cttgatgtag ccctggatcg acgtgagggg cgtgcgcagc
      661 tcgtgggaga ccatggccac gaaatcgtcc ttcatgcggt tgagctcctg ctcctgttcc
      721 agcgagcgct ggagttcctg ggcgagtcgc gcgttctcca gggcgagacg cgtgccccac
      781 cacaacgcca ccgccggcgc cgccgccagc gggatcaacc acaggctctc gtccacggcg
      841 gcgaccacga cgggcgccag cgcgagcagt ggcagggtcg cctgcgcctg gaagacgagg
      901 tccttcgcga tgtagggatt catggccacg ccgctcgaca tcgccacggc gacgtcggtg
      961 atgatcgtgt tcagcaggaa gaacgtgatc gcggaggcga ggaacggcag gatgctgccc
     1021 gagttcagcg tcatcccgcc gccgagcccg tggtagacgg cccccgcggc cccgagcgac
     1081 agcgcgaact gtcccgcgtt gaacgccacc ttccacaggc tcttgtgcag gaggatgtcg
     1141 gcgatcatcg aggcgaccac ctgggcgacg acggccgccg gtgtcccgaa gccgatcatc
     1201 aatgcgaacg cgaacgtcgt ggatgcggtg agctcgtcca cctcgccccc gcgggggacc
     1261 gagatcggga agacctcgcc caccaacagc agaagcgcca ggagcccgaa tgcgcgctcg
     1321 ctcgccgcgg cttcgagcga cggctgcgcg acgaaccaga ccagcagcga acagcccgcg
     1381 atggtgacga gcgcaacgta cgtgtcgaat gaacggcctc gaacggtcat ctggattccg
     1441 ttctgtcttc ggcccgtccc gggcctgacc gaagggctga cccagcttcc ctgcggggga
     1501 acgcgttcct caggtcagga ggggtcgcga tgaggatcga ggaacccgga caccgcggcc
     1561 aggtacgcgt cctgctcctc cacgaacgcc atgtgaccgc tgcgctcgaa gacgacgagt
     1621 tcggaccgcg ggaccgaggc ggcgatcgcc tcggccgctt cgggcacgca cgtccggtca
     1681 tgccgtccgg cgagcacgag catcggggag gccacctcgt ccaggcgatc ctccagctcg
     1741 atgtcgccgg cgtcggtggt ggcgaagtgg cggagcacgt cgggggcgta gacggtgtcc
     1801 gacgtgcgcg ccaggtagtc gtcgatgcgt gggtccaccg ggtcggcgaa atggaagggg
     1861 aactgatcca ccatcagcga ggcgaactcc tccggtgtct ggacctcggg ttccctcgcc
     1921 caggagtcgg ccacctgtcg gcggagcgac accggctcga acgacagcag gttgggctcc
     1981 accgcttcga accatcgcat ggaagccaca ccggacgaga cgatggtctg tgccgccatc
     2041 ccttggtagt ccacggcgtt ctgcaacgcg acgaaggcgc cgaaggaatg gccgaggacg
     2101 gcgtagcggt ccagccggag cgcgcgggcg agcatgatca cgtcctgggc gtgccgttcc
     2161 aaggtccagg aacgcacggg aggcacctcc gagcggccac atgcgcggag gtcgacgagc
     2221 agcagccggt agccacgctc ggtcagcgga tcgaggtagt cggcgaactc gtgatggtcg
     2281 aggccgggtc cgccgtgcaa cacgatcacc gggtagccct cgccgcgatc gaccacccac
     2341 aacgcggtgt cgtcgatgcg gaccagagcg ccctcatcgg tcacggaggc gagccccacc
     2401 cctcacggcc cacgatgcgg aacccggagt ccttggtctt gtaccgccgg ttcacgttga
     2461 tcaggagcgg tgtgagcgtc tcggtgatgc gtgccatcga cagcgcgccg caatcgatcc
     2521 accggaggtc ggggatgtcg tcgatcagac caccgatcac cgtcttggcg tcgtcgtcgt
     2581 cgccgcacac cagcacatcg gaatccaggg gatgttcgag gtcggcgagc gcgtgcgcgg
     2641 cgatcgtgtg gaacgccgcg accacgcgcg gaccctcgcc cagcagcgcg gaggcctgtt
     2701 cggccgccga tccgtgccac gggcgcacga cctgccaggc gcgcccaccc accgcggtgg
     2761 caagggggct ggtcgcgtcc atcacgatcg cgcccggccg aacgctcccg gcgatcgatc
     2821 ggtagatgtc tgcctgtccg gcgaacggga cggtgaccat cagcacgtcg acggaagcag
     2881 ccgcgtcctc gttcgtggtg ccggtgacct gggcggtatc ggccagcgtc tcg
//
LOCUS       DATDBZ010000377         3468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1969563, whole genome shotgun sequence.
ACCESSION   DATDBZ010000377 DATDBZ010000000
VERSION     DATDBZ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969563"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VIX62_09020"
     CDS             complement(<1..949)
                     /locus_tag="VIX62_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY5860410.1"
                     /translation="MDFAEALAELDSRKEGRMVPDLSRILALATYLSDPQLTYPTIHV
                     TGTNGKGTAAKVVTELACAHGITTGLYTSPHLSSVTERFSVCGVDMTQDEFADAYTYL
                     LPYLQLVDEQGEERVTYFEALTALAYLWFADKPVGLGVYEVGMGGLWDATNLVASDVA
                     IITPIGLDHRELGSTITEVAREKAGIIKEGKIAVVREQETEALDVLQARCDDVGAAML
                     REFRDWEVEDRLLAVGGQSFLLRGVHGTYEDVYLPMFGEYAVRNAAGAIVALEAFVDG
                     PLDDTSMHAALDGVRWPGRLEIVSRKPTILLDGAHNPAGA"
     gene            complement(958..>3468)
                     /gene="valS"
                     /locus_tag="VIX62_09025"
     CDS             complement(958..>3468)
                     /gene="valS"
                     /locus_tag="VIX62_09025"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY5860411.1"
                     /translation="LEAKWDEWWEASGTYRFDPTKSREQIYAIDTPPPTVSGSLHVGH
                     VMSYTHTDLMARFKRMRGFEVFYPMGWDDNGLPTERRVQNYFGVRCDPGLSYDPSFDP
                     STLERPDGEAPVAVSRSNFIELCGRLTVEDEKAFEALFRRLGLSVDWTRTYTTIGERS
                     RRISQRSFLRLLRDGHAYTAEAPTLWDIDFQTAVAQAEIEDRERDGVYHRITFDREDG
                     GTVEIETSRPELIPACVALVVNPSDDRFASLVGTTASTPLFRVPVPVVAHELADPEKG
                     TGVAMICTFGDVTDVTWWKELGLPARVVMQKDGTIGGRTFGEPGWESRDPEAANAAMG
                     ELAGLHAKKARARIADLLRDAGHLVGEPQPVKHVVKFYEKGDRPLEVITSRQWFVHTL
                     ALKDRLLARGEQIAWHPAFMGGRYASWVEGLNSDWAMSRQRFFGVPFPVWYPLDEHGN
                     ARHDDPILADESALPIDPQDAVPPGYEASQRGEPGGFVGDPDIMDTWATSSLSPQIAA
                     HWEDDPDLFARLFPMDLRPQAHDIIRTWLFYTVLRAELQHDSLPWTNAAISGWVLDPD
                     RKKMSKSKGNAVVPTEVFEQHSADAVRYWAGSARLGVDTAFDERQMKVGRRLAIKILN
                     ASRFALSMEGEPGAITEPLDRSMLTALRAVVVEATEAFEAYEHAKALDVIERFFWGFT
                     DDYLELVKQRAYGGHGPEGAASALGALRTALQTFLRLFAPFLSYVTEEVWSWWQTGSV
                     HREAWPTAEELPDDGDPGVYAVAADVLTAVRKEKAMQKVSLRAPASRVTVHDTQDRLR
                     LLTQASLDIREAGTIQALDQEEASEFSVQTELAPVADA"
BASE COUNT          521 a         1207 c         1185 g          555 t
ORIGIN      
        1 cggccccagc agggttgtgc gcgccgtcca acaggatcgt gggtttgcgc gacacgatct
       61 ccaggcggcc gggccagcgc acgccgtcca gcgccgcgtg catcgaggtg tcgtcgaggg
      121 ggccgtccac gaaggcttcg agcgcgacga tcgccccggc cgcgttccgg accgcgtact
      181 ccccgaacat cggcaggtac acgtcctcgt aggtgccgtg cacccctcgc agcaggaacg
      241 actgcccgcc caccgccagg agacgatcct cgacctccca gtcgcggaac tcccgaagca
      301 tcgcggcacc gacgtcatcg caccgggcct ggagcacgtc gagcgcctcg gtctcctgtt
      361 cccgcacgac cgcgatcttg ccctccttga tgatcccggc cttctcacga gccacctcgg
      421 tgatcgtcga gccgagttcg cggtggtcga gtccgatggg cgtgatgatg gcgacgtcgc
      481 tggcgacgag gttcgtggca tcccacaggc cgcccatccc cacctcgtac accccgaggc
      541 ccaccggctt gtcggcgaac cacagatacg ccagcgcggt caacgcctcg aagtaggtga
      601 cgcgttcctc accctgctcg tccacgagct gcaggtaggg cagcaggtag gtgtaggcat
      661 ccgcgaactc gtcctgggtc atgtccacgc cgcacaccga gaaccgttcg gtcaccgacg
      721 acagatgcgg cgaggtgtag agccccgtgg tgatgccgtg cgcgcacgcg agctcggtga
      781 ccaccttggc ggcggtccct ttgccgttcg tgccggtcac gtggatcgtg ggataggtga
      841 gctgcgggtc ggacaggtag gtcgccagcg ccaggatgcg cgagaggtcg ggcaccatgc
      901 gcccttcctt gcgcgagtcg agttcggcga gcgcttcggc gaagtccacg tgggtgctca
      961 ggcgtccgcg accggcgcca gttccgtctg gaccgagaac tcggacgcct cttcctggtc
     1021 gagcgcctgg atcgtgccgg cctcacggat gtcgagggag gcctgggtga gcagacgcag
     1081 ccggtcctgc gtgtcgtgga ccgtgacacg gctcgccggc gcgcgcagcg acaccttctg
     1141 catcgccttc tccttgcgca ccgcggtgag cacgtcggcg gccaccgcgt agacgccggg
     1201 atcgccgtcg tcgggcaact cctcggcggt gggccaggct tcgcggtgca cgctgccggt
     1261 ctgccaccac gaccagacct cctcggtgac gtaggacagg aacggcgcga acaggcgcag
     1321 gaacgtctgc agcgctgtgc ggagcgcgcc gagggccgac gcggcgccct cggggccgtg
     1381 accgccgtag gcgcgctgct tcaccagctc gaggtagtcg tcagtgaagc cccagaagaa
     1441 gcgctcgatc acgtcgaggg ccttggcgtg ttcgtaggcc tcgaacgcct cggtggcctc
     1501 cacgacgacg gcccgcagcg ccgtgagcat cgagcggtcg agcggctcgg tgatcgcacc
     1561 gggctcgccc tccatcgaca acgcgaaccg tgacgcgttc aggatcttga tcgccagccg
     1621 gcgacccacc ttcatctgcc gctcgtcgaa cgcggtgtcc acgcccagcc gcgcgctgcc
     1681 cgcccagtag cggacggcgt cggcgctgtg ctgttcgaac acctcggtgg gcaccacggc
     1741 gttgcccttg gacttcgaca tcttcttgcg atcggggtcc agcacccagc cgctgatcgc
     1801 ggcgttcgtc cagggcagcg agtcgtgttg cagctcggcg cgtagcaccg tgtagaagag
     1861 ccaggttcgg atgatgtcgt gcgcctgggg gcgaagatcc atcgggaaca gccgggcgaa
     1921 caggtcgggg tcgtcctccc agtgcgccgc gatctgcggc gacagcgacg aggtcgccca
     1981 cgtgtccatg atgtcggggt cgcccacgaa cccgccgggc tccccgcgtt gggacgcctc
     2041 gtagccgggc ggcaccgcat cctgcggatc gatcggcagg gcggactcgt cggccaggat
     2101 cgggtcgtcg tgacgggcat tgccgtgctc gtcgagcggg taccagacgg ggaacggcac
     2161 gccgaagaac cgctggcggc tcatcgccca gtccgagttc aggccctcga cccacgaggc
     2221 gtagcgcccg cccatgaacg ccggatgcca ggcgatctgt tcgccgcggg ccagcagccg
     2281 gtccttgagc gccagggtgt gcacgaacca ctgccggctg gtgatgacct cgagcggtcg
     2341 gtcgcccttc tcgtagaact tcacgacgtg cttcaccggt tgcggttcgc ccaccaggtg
     2401 accggcatcg cgcagcagat ccgcgatccg ggcacgcgcc ttcttggcgt gcaggcccgc
     2461 gagttcgccc atggcggcgt tggcggcttc cgggtcgcgg gactcccagc cgggctcgcc
     2521 gaacgtgcga ccgccgatcg tgccgtcctt ctgcatcacg acgcgggccg gcaggccgag
     2581 ctccttccac cacgtgacgt cggtgacgtc gccgaaggta cagatcatgg cgacgccggt
     2641 ccccttctcc gggtcggcga gctcgtgcgc cacgaccggc accggcaccc ggaacagcgg
     2701 ggtcgacgcg gtggtgccga cgagcgaggc gaaccgatcg tccgacgggt tcaccacgag
     2761 cgcgacgcac gcggggatca gctccggacg gctggtctcg atctcgaccg tcccgccgtc
     2821 ctcgcggtcg aacgtgatgc ggtggtaaac gccgtcgcgt tcgcgatcct cgatctcggc
     2881 ctgcgccacc gccgtctgga aatcgatgtc ccacaacgtg ggcgcctcgg ccgtgtaggc
     2941 gtgtccatcg cgcagcaacc ggaggaacga acgctgggag atccgccgcg accgttcacc
     3001 gatcgtggtg taggtgcgcg tccagtccac cgacagcccc agccgccgga agagcgcctc
     3061 gaaggccttc tcgtcctcga cggtgagcct gccgcagagc tcgatgaagt tgctgcggct
     3121 caccgcgacg ggcgcctcgc catcgggtcg ttccaacgtc gacggatcga acgacgggtc
     3181 gtaggacagg ccggggtcgc agcggacccc gaagtagttc tggacgcggc gctctgtggg
     3241 caggccgttg tcgtcccagc ccatcgggta gaagacctcg aaccctcgca tgcgcttgaa
     3301 ccgcgccatg aggtcggtgt gcgtgtagct cattacatgg cccacgtgca gcgagccgct
     3361 caccgtgggc ggcggcgtgt cgatcgcgta gatctgctcg cgtgacttcg tggggtcgaa
     3421 ccggtaggtg ccggaggcct cccaccattc gtcccacttg gcttccag
//
LOCUS       DATDBZ010000378         2390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1970483, whole genome shotgun sequence.
ACCESSION   DATDBZ010000378 DATDBZ010000000
VERSION     DATDBZ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970483"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..904)
                     /gene="hemQ"
                     /locus_tag="VIX62_09030"
     CDS             complement(227..904)
                     /gene="hemQ"
                     /locus_tag="VIX62_09030"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: protein motif:HMM:NF042928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEY5860412.1"
                     /translation="MSETLYALYPVFRVKDDLREALTQNDERRHAAQEIDDLYTSWEG
                     RVHVRGTYSTVGFRADSDLMLWLHGSSPEDMQQALVEFRRTTVGRLTDLTWTFMGAVK
                     PAEFTADHAPAFVKGTPPETFLCVYPFVRTPEWYLLPREERGELLREHGGAGREFPEV
                     LANTTMAFGLGDWEWILAFEAARADSLVDCIRRLRETKARLFTKVEIPFVTGIRKSVA
                     DVLDDLV"
     gene            complement(901..2277)
                     /gene="hemG"
                     /locus_tag="VIX62_09035"
     CDS             complement(901..2277)
                     /gene="hemG"
                     /locus_tag="VIX62_09035"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEY5860413.1"
                     /translation="MNPSSRPRRVAVIGGGVTGLTVAYRLVHQDAALEVTVYEGDARP
                     GGKLCSVQVGSLTLPAGADSFLARKPWGVALCKELGVELEAPGTTGAHLWTETGLVPL
                     PKDAPFGIPGDIGEVLRWPGVSSKGRRRAAQDLLRKKRKDGVEESLGGLLRRRLGDEA
                     TDRAVAPLLAGLYAGDVDRLSARATFPDLERWESWQGSLIRGAQAATRHSKRGDPGPM
                     FVRPRGGVDRLIEAIAERLGPRVRTGSERATLEGLDADAVVVATSAGEAAGLLEGSAP
                     VAAADLAGIPYASTGVVLMVYGEDTQRGLPKGTGFVVPRGEAPMTAATWLSSKWPDES
                     FGTRAVVRSFVGAVGEQDILNADDDELVDACARHLTALVSLPDRPEHAAVVRWPASMP
                     QYDVGHQGRVARIRASLPAGIFVVGQAYDGVGIPDCVRAAGETAAQVLAYLDDDAKPT
                     PNDEETVR"
BASE COUNT          393 a          869 c          777 g          351 t
ORIGIN      
        1 tgtcacgcga tgacgtcggt ccacgacccg acccgcccga cagcgatgag gatctggtcg
       61 cggggttccg cgaaggcgcg gacgcgctcg ttcgggccct cgagatcgcc gagcgtccat
      121 ccaggacatg acggcacacg cgtggtgaga tcacctcgat cggcagcgga cgagaaggcc
      181 tcgctctcac gccggatcac ctcgacgaag tcgagcgccg ccaccgtcac accaggtcgt
      241 cgagcacgtc ggcgaccgac ttgcggatgc ccgtgacgaa cgggatctcg accttcgtga
      301 agagccgcgc cttggtctcg cggagccgtc ggatgcaatc gaccaacgag tcggcgcgag
      361 cggcttcgaa cgccaggatc cactcccagt cgccgagccc gaaggccatg gtggtgttcg
      421 ccaacacctc ggggaactct ctgccggccc caccgtgttc gcggagcagt tcgccacgct
      481 cctcgcgcgg caacaggtac cactccggcg tgcgcacgaa cgggtacacg cacaggaacg
      541 tttcgggtgg cgttcccttc acgaaggccg gcgcatggtc ggcggtgaac tccgcgggct
      601 tcaccgcgcc catgaacgtc cacgtcagat cggtgaggcg gccgaccgtg gtccgccgga
      661 actccaccag cgcctgctgc atgtcctcgg gtgaggaccc gtgcaaccac agcatcagat
      721 ccgagtcggc ccggaacccc accgtcgagt acgtgccgcg cacatgcacc cggccctccc
      781 atgacgtgta gagatcgtcg atctcctgcg ccgcgtgacg gcgttcgtcg ttctgagtca
      841 gcgcctcacg gaggtcatcc ttgacccgga acaccgggta gagcgcgtag agcgtctcgc
      901 tcatcgaacc gtctcctcgt cgttcggggt gggtttcgcg tcgtcgtcga gataggcaag
      961 cacctgtgcc gccgtctcgc cggcggcacg aacacaatcg gggatgccca cgccgtcgta
     1021 ggcctgcccc accacgaaga tacccgcggg taacgatgca cggatgcgcg cgacacgacc
     1081 ctggtgtccc acgtcgtact gcggcatcga cgccggccac cgcaccacgg ccgcgtgctc
     1141 gggtcggtcg ggcagcgaca cgagcgcggt cagatgccgc gcacacgcat cgacgagctc
     1201 gtcgtcgtca gcgttcagga tgtcctgctc gccgaccgcg cccacgaacg agcggaccac
     1261 ggcccgggtc ccgaaggact cgtcgggcca cttgctgctg agccaggtgg ccgccgtcat
     1321 cggggcctcg cctcgcggca ccacgaaacc ggttcccttc gggagccctc gctgcgtgtc
     1381 ctcgccatag accatgagca cgaccccggt cgacgcgtac gggatccccg ccaggtcggc
     1441 ggcagcgacc ggtgcggacc cctccaacag ccccgcggcc tcgccggctg aggtggcgac
     1501 cacgaccgcg tcagcgtcga gcccttcgag cgtcgctcgc tcggacccgg ttcgaacccg
     1561 tgggccgaga cgctcggcga tcgcctcgat gagccgatcg acacccccac gcggtcgcac
     1621 gaacataggg ccggggtcac cccgcttgga gtgtcgagtg gctgcttggg cacctcggat
     1681 caagctccct tgccaggact cccagcgctc cagatcgggg aaggtcgcgc gggccgaaag
     1741 gcggtcgacg tcgccggcgt agagccccgc gagcaacggc gccaccgctc ggtcggtggc
     1801 ctcatcgccc agccgccgtc ggagcagacc cccgagcgac tcttcgacgc cgtccttgcg
     1861 tttcttccgg agcaggtcct gcgcagcgcg acgccggccc ttggacgaga cccccggcca
     1921 gcgcagcact tcgccgatgt cacccgggat accgaacggt gcgtccttgg gcagcggcac
     1981 caggccggtc tcggtccata ggtgtgcgcc ggtcgtgccc ggtgcctcga gttcgacccc
     2041 caattctttg cacaaggcga cgccccaggg cttacgcgcc aggaacgaat cggcgccggc
     2101 gggcagcgtc agggaaccga cctggacact gcagagcttg ccgccgggac gagcgtcacc
     2161 ttcgtagacg gtcacctcaa gcgcggcatc ctggtgcacc aaccgatagg cgacggtcaa
     2221 ccctgtgaca ccaccaccga tgacggcgac ccggcgaggc cggctcgatg ggttcactcg
     2281 acggagaccc cggccgcgag ttggtcacgc acgacctgcg ccaacacgtc caagtagtcg
     2341 gggtcggcgt tgggcatccg tgtgcgcgcg aacgcgatcc ctgcatcgcg
//
LOCUS       DATDBZ010000379         5687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2785521, whole genome shotgun sequence.
ACCESSION   DATDBZ010000379 DATDBZ010000000
VERSION     DATDBZ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2785521"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VIX62_09040"
     CDS             complement(<1..1064)
                     /locus_tag="VIX62_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEY5860414.1"
                     /translation="MTERYWRAAEPKSSYDVVIVGAGGHGLATAYYLAANHGITDVCV
                     VEKGWLGGGNMARNTTIIRSNYLWDESAAIYEHSLKLWEGLEEALSFDMQFSQRGVLN
                     LAHDLGDIRTSKRRVYANALNGVDAEWLDTDEVKAFVPVVNVSPDVRYPVLGGTLQRR
                     GGVARHDKVAWGFARGADRLGVDIVEHAEVTGFVRGASGEVQGVETSRGTVRAGKVAL
                     AAAGHTSVLASMVGLRLPLQSHPLQALVSALYEPVLGCVVMSNAVHVYVSQADKGELV
                     MGAGIDAYNGYGQRGSFHVIEHQMAAALELFPMFAHASMLRTWGGIVDVCPDASPIIG
                     PTPIPGLFLNCGWGTGGFKAT"
     gene            1211..1999
                     /locus_tag="VIX62_09045"
     CDS             1211..1999
                     /locus_tag="VIX62_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY5860415.1"
                     /translation="MRDQPGSNLQSVRRALRALELISESGECGVTELGRKLGVHKATA
                     SRLVATLAERGFVERDPITEKYRLGFGLIALAGAAVGANDLVRSARPILEDLAERTRE
                     TVNVGVLSGDAVVYVDQVAGTRSIVSVNWVGQRTPLHCTSNGKVLLAFMREDARDRLL
                     AEPLPRLTPRTITDPERLRAQLTEIRARGYAQTMDELEEGLNAVAAPIRGMNGDLIAA
                     LSVSGPAFRMRGVDLPRIGRATAEAAAVVSRRLGHVDRGRPAAG"
     gene            2019..3059
                     /locus_tag="VIX62_09050"
     CDS             2019..3059
                     /locus_tag="VIX62_09050"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEY5860416.1"
                     /translation="MTTTWDAHRSELPTLLGAIGRTPLVRLQRVTPPGVDILAKVEWY
                     GPTGSVKDRIYAHMLTKAEERGALKPGMTIIECTTGNAGIACSAVAAIKGYRCVIVMP
                     EGMSSERKTMIKAYGADLVLTPGAGTDIDLAIARMREIVADDPDRYFFPGEFENPDNP
                     EAQLASGLEIWEQSGGRVDAVVGAQGTGGWISGVTRALKRHNPDVRAFAVEPAECPLI
                     SEQRWGTHGVPGIGDGIVPPNLDLAAMDGIVTVSTQEALATARRLAQEEGLLCGPSSG
                     INVAAVHKVSERHPELRRIVTVVCDTGQRYLSGELYGESTQVEEPERDHTIDATTAAE
                     IDRHRDRLEFIA"
     gene            complement(3075..4007)
                     /locus_tag="VIX62_09055"
     CDS             complement(3075..4007)
                     /locus_tag="VIX62_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEY5860417.1"
                     /translation="MKLRRRNSSHVIDRRRAGGGGSLPGLGGGGGGFPGLGRGGGGGL
                     PIGGKGGGLLLIVVIVIFAVCIAPKIFGGDSGFSGDIFSGLGQAQDGTGIDPANDPDR
                     STKQFVNDVVSDVEETWGQLFAGSNTTFRPADLILFTGTTQTGCGQGSAATGPFYCPA
                     DSKIYLDMSFFQELEQRFGAPGDFAQAYVIAHEYGHHIQNLLGINAQVQQQSQQDPGR
                     ANDLSVRLELQADCLAGVWGHSLTSDELGPDEVDEALTAAAAIGDDRIQQSTTGRIDP
                     ETWTHGSSEQRVQWFRTGFDSGDPNACDTFNTDI"
     gene            4078..5493
                     /locus_tag="VIX62_09060"
     CDS             4078..5493
                     /locus_tag="VIX62_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5860418.1"
                     /translation="MKEPWSLTAADALAAMRAKDLSPVELLESVIGRAEAVEPCVNAF
                     AERRFEQALTSAKTSADRYVAGSRIRPLEGLPVALKEEVPVRGWQMRYGSLAIDEVAE
                     TTSPLAERILKAGAVVHARTTTPELSCAGYTHSKLWGVTRNPWNTEVTVGGSSGGSGA
                     SLAAGSSVLASGSDIGGSIRLPSSMNGVVGFKPPHGRVPVDPPFNLDRYCHDGPMART
                     VTDCALFENVLAGPHPMDVTSLRPKLRIPSTLKPIEGWRVAVCVNLGAWNVDAEVAAN
                     TRAVGEALRDAGALVEEVELPWTMDQIMATGRRHFGMTFGAEIAEHVAAHPDHVNDYL
                     EAWAVEAATTLAEPGAFLHGLEGEQAMWEPLGELFRRYRALIAPTWAVTGIPAGDSFL
                     GQLTPNGGPNDRQFSVMMTTPFNILSPCPVLAVPSGVAANGVPTGVQIVGRTYSDVDV
                     FRIGAALERVRSWTGLAPLSP"
     gene            5490..>5687
                     /locus_tag="VIX62_09065"
     CDS             5490..>5687
                     /locus_tag="VIX62_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860419.1"
                     /translation="MTTYDLYLDHGPKRKKTMVHVIGLMGCVHNGPTTDQVLDETPEA
                     IRGFLAFLAEQGDPVDPEAPFD"
BASE COUNT          882 a         1940 c         1993 g          872 t
ORIGIN      
        1 gtcgccttga agcctccggt gccccagcca cagttcagga acaagccggg gatcggtgtg
       61 gggccgatga tcggcgatgc gtccgggcac acgtcgacga tgccgcccca cgtccgcagc
      121 atcgaggcgt gcgcgaacat cgggaacagc tccagcgcgg cggccatctg gtgttcgatc
      181 acgtggaacg aaccccgctg tccatagccg ttgtaagcgt cgatgccggc gcccatcacg
      241 agttcgccct tgtcggcctg gctcacgtag acgtgcacgg cgttggacat cacgacgcag
      301 ccgagtaccg gctcgtagag cgcgctcacc agcgcctgca acgggtgcga ctgcagcggc
      361 aaccgcaagc cgaccatcga tgcgagcacg ctggtgtggc ccgccgccgc caacgccacc
      421 ttccctgccc ggaccgtgcc ccgggaggtc tcgacgccct ggacctcgcc gcttgcgccg
      481 cgcacgaatc cggtgacctc ggcgtgctcg acgatgtcga cacccagtcg atcggcgccg
      541 cgggcgaacc cccacgcgac cttgtcgtgg cgtgcgacgc ccccgcgccg ttgcagcgtg
      601 ccgccgagca ccgggtagcg cacgtccggc gagacgttca cgacgggcac gaacgccttg
      661 acctcgtcgg tgtcgagcca ctccgcgtcg accccgttga gggcgttggc gtagacgcgg
      721 cgtttggacg tacggatgtc gcccaggtcg tgggccaggt tcaagacgcc acgctgggag
      781 aactgcatgt cgaacgagag tgcttcctcg aggccctccc agagcttcag ggagtgctcg
      841 tagatcgcgg ccgattcgtc ccagaggtag ttggaacgga tgatcgtggt gttgcgggcc
      901 atgttgccgc cgccgagcca gcccttctcc accacgcaga cgtcggtgat gccgtggttg
      961 gcggcgaggt agtaggcggt cgccaggccg tgaccgccgg cccccacgat cacgacgtcg
     1021 taggaggact tcggttcggc tgcgcgccag taccgctcgg tcatccgcga cagggtaggc
     1081 cgtggggcgg cacgcggcga tgcgagcggt gcgcggtgcg caacgttcgg gccagccccg
     1141 tatcgacaag ccacgcgcat cggcgtacca tccgacgccg tcgttgcgga caggtccgag
     1201 gtgttgcggt atgcgagacc aaccgggttc gaacctccaa tcggtgcgcc gcgcgttgcg
     1261 agccctcgag ctgatctccg agtccggcga atgcggggtc accgagctcg ggcgcaagct
     1321 cggcgtgcac aaggcgaccg cctcccgcct ggtcgccacc ctcgccgaac gggggttcgt
     1381 cgaacgcgat cccatcaccg agaagtaccg cctcgggttc ggtctgatcg cccttgcggg
     1441 ggcggccgtg ggggccaacg acctggtgcg gagcgcgcgc cccatcctcg aagacctcgc
     1501 tgagcgcacc cgcgagaccg tcaacgtggg cgtcctctcg ggtgacgcgg tggtctacgt
     1561 cgaccaggtg gccggcaccc gctcgatcgt cagcgtgaac tgggtgggcc agcgcacgcc
     1621 cctgcactgc acgtcgaacg ggaaggtgct gctggcgttc atgcgcgagg acgcccgcga
     1681 ccgtctgctc gccgagcccc tgccgaggct cacaccccgc acgatcaccg atccggagcg
     1741 tctccgcgcc cagctcaccg agatccgtgc ccgtggctac gcacagacga tggacgaact
     1801 cgaagagggc ctcaacgccg tggccgcgcc gatccgtggg atgaacgggg acctgatcgc
     1861 cgcgctcagc gtttcggggc cggcgttccg gatgcgaggc gtcgacctgc cgaggatcgg
     1921 ccgggccacc gccgaggccg ccgcggtggt ttcgcgccgc ttgggtcatg tggatcgcgg
     1981 gcgtcccgcc gcgggatgag acgcaggagg atcgcacgat gacgaccacg tgggatgcgc
     2041 accgctccga gctcccgacg ttgctgggtg cgatcggccg gaccccgctg gttcggctcc
     2101 agcgcgtgac cccacccggc gtggacatcc tcgccaaggt cgagtggtac gggccaacgg
     2161 gatcggtgaa ggaccgcatc tacgcccaca tgctcacgaa agccgaggag cggggcgcgc
     2221 tcaagcccgg gatgacgatc atcgagtgca ccaccggcaa cgccgggatc gcctgctcgg
     2281 cggtggcggc gatcaagggg taccggtgcg tcatcgtgat gcctgaaggc atgagttcgg
     2341 agcgcaagac gatgatcaag gcgtacggtg cggacctggt gctcacgccc ggcgccggca
     2401 ccgacatcga cctggcgatc gcccggatgc gcgagatcgt cgccgacgac cccgaccggt
     2461 acttcttccc cggggagttc gagaaccccg acaaccccga ggcccagctc gcgagcggtc
     2521 tggagatctg ggagcagagc ggcggtcgcg tcgacgccgt cgtgggcgcc caggggaccg
     2581 gcgggtggat cagcggcgtg acgcgggcgc tgaagcggca caacccggat gtgcgtgcgt
     2641 tcgcggtgga gcccgcggaa tgcccgctga tcagcgagca acgatggggt acccacggtg
     2701 tccccgggat cggcgatggc atcgtgccgc cgaacctcga cctcgcggcg atggacggca
     2761 tcgtcaccgt ctccacgcag gaagcgttgg cgaccgcgcg ccgcctggcc caagaggaag
     2821 ggctcctctg cggcccgtca tctggaatca acgtcgcggc ggtgcacaag gtgagtgagc
     2881 ggcaccccga gctgcgccgg atcgtcacgg tggtctgcga caccgggcag cggtacctgt
     2941 cgggggagct ctacggcgaa tccactcagg tcgaggaacc cgaacgcgat cacacgatcg
     3001 acgccaccac ggccgccgag atcgaccggc accgcgaccg gctcgagttc atcgcctgag
     3061 actgttggcg ccggctagat gtcggtgttg aacgtgtcgc aggcattggg gtcgcccgag
     3121 tcgaaccccg tccggaacca ctgcacccgc tgttccgagg acccgtgggt ccaggtctcc
     3181 ggatcgatgc gacccgtcgt ggactgctgg atgcggtcgt ccccgatcgc cgccgctgcc
     3241 gtgagcgctt cgtcgacctc gtccggcccg agttcgtcgc tggtcaacga gtgcccccac
     3301 acgccggcga ggcagtcggc ctggagttcc agccggaccg acaggtcgtt cgcccgaccc
     3361 ggatcctgct gtgactgctg ctgcacctgc gcgttgatcc ccagcaggtt ctggatgtgg
     3421 tggccgtatt cgtgggcgat cacgtaggcc tgcgcgaagt cgcccggtgc gccgaaccgc
     3481 tgttccagct cctggaagaa gctcatgtcc aggtagatct tcgaatcggc cgggcagtag
     3541 aaggggccgg tcgccgcgga cccttgcccg cacccggtct gcgtggtgcc ggtgaacagg
     3601 atcaggtcgg ccggccggaa cgtcgtgttc gagccggcga acagctggcc ccaggtctct
     3661 tccacgtcac tcacgacgtc gttcacgaac tgcttggtgg accggtcggg atcgttcgct
     3721 gggtcgatcc cggtgccgtc ctgcgcctga cccagtccgg agaagatgtc gccgctgaaa
     3781 ccgctgtcgc cgccgaagat cttcggcgcg atgcagacgg cgaagatcac gatgaccacg
     3841 atcaggagca gtccgccgcc cttgccgccg atcgggagcc cgccgccgcc gcctcgaccg
     3901 aggccgggga acccaccacc gccaccgccc aggcccggca aggatccgcc gccaccggcg
     3961 cggcggcgat cgatgacatg gctgctgttg cggcgtcgaa gcttcatggt ccatcttccc
     4021 ttcccctgct cggcgatgtc gaaccccgaa ccgctgcagc tcggataggg tgcccggatg
     4081 aaggaaccgt ggagcctcac cgccgcggac gccctggccg ccatgcgggc gaaggacctc
     4141 tcgcccgtcg agctgctgga gtcggtgatc ggccgtgccg aagcggtgga accctgcgtc
     4201 aacgcgttcg cggagcgacg gttcgagcag gcgctgacat cggcgaagac gtcggccgac
     4261 cgctacgtgg ccgggtcccg gatccggccg ctggagggtc tgccggtcgc tctgaaggaa
     4321 gaggtccccg tccgcggctg gcagatgcgg tacgggtcgc tcgcgatcga cgaggtcgcg
     4381 gagacgacgt cgccgctggc cgaacggatc ctcaaggccg gtgcggtggt gcacgctcgc
     4441 accacgacgc ctgaactctc gtgcgccggc tatacccact cgaagctctg gggcgtgacc
     4501 cgcaacccgt ggaacaccga ggtcaccgtg ggtggctcga gcggtggatc gggagcgtcg
     4561 ttggcggcgg gatcgtcggt gctcgcgagc ggttcggaca tcggaggttc catccggctg
     4621 ccgtcctcga tgaacggcgt tgtggggttc aagcctccgc acggccgtgt gccggtcgat
     4681 ccgccgttca acctggaccg gtactgccat gacggtccga tggcacgcac ggtgaccgac
     4741 tgcgccctgt tcgagaacgt gctcgccggc ccgcacccga tggacgtcac gtcgctgcga
     4801 ccgaagttgc ggatcccgtc gacgttgaag cccatcgagg gttggcgcgt ggcggtctgc
     4861 gtgaacctcg gggcatggaa cgtggacgcc gaggtggcgg cgaacacccg ggcggtgggg
     4921 gaggcgcttc gagatgcggg cgcgctcgtc gaggaggtcg agctgccgtg gacgatggat
     4981 cagatcatgg ccaccggccg gcggcatttc ggcatgacct tcggggccga gatcgccgag
     5041 cacgtcgccg cccaccccga ccacgtcaac gactacctcg aagcctgggc ggtggaagcg
     5101 gccaccacgc tggccgaacc cggcgcgttc ctgcacgggc tggagggtga acaggcgatg
     5161 tgggagccgc tcggcgagct gttccgccgc taccgcgcgt tgatcgcacc gacgtgggcg
     5221 gtgaccggga tcccggccgg cgacagcttc ctcggtcagc tcacgcccaa cggtggtccc
     5281 aacgaccggc agttctccgt gatgatgacc acgccgttca acatcctgtc gccgtgcccc
     5341 gtgctggcgg tgccgagcgg ggtcgcggcg aacggtgtgc ccaccggcgt gcagatcgtg
     5401 ggccggacct actccgatgt cgacgtgttc cggatcggcg ccgcgctgga gcgcgttcga
     5461 tcctggaccg ggctcgcgcc cctttcgcca tgacgaccta cgacctgtac ctcgatcacg
     5521 gcccgaagcg gaagaagacc atggtgcacg tgatcggcct gatggggtgc gtgcacaacg
     5581 gccccacgac cgatcaggtg ctggacgaga cgccggaggc gatccgtggc ttcctggcct
     5641 tcctcgccga gcagggcgac ccggtcgatc cggaggcgcc gttcgac
//
LOCUS       DATDBZ010000380         3038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_947325, whole genome shotgun sequence.
ACCESSION   DATDBZ010000380 DATDBZ010000000
VERSION     DATDBZ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947325"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VIX62_09070"
     CDS             <1..749
                     /locus_tag="VIX62_09070"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071065.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HEY5860420.1"
                     /translation="PEATGYGAVYFAQEMLATRDDSLEGKTCLVSGAGNVAQYTVEKL
                     LHLGAKPVTLSDSGGFIHDPDGIDEEKLAWVMDLKNERRGRIMAYADRFPAATFTPAD
                     PSDDHNALWEVPADCAFPSATQNEINGKDAANLIGGGVRVVSEGANMPTEPEGVDQFV
                     TAGVLYGPGKAANAGGVAVSGLEMSQNAMRLSWTREGVDERLREIMRRVHEQCRDTAE
                     EYGEPGNYVHGANIAGFRKVADAMLAQGVI"
     gene            868..1374
                     /locus_tag="VIX62_09075"
     CDS             868..1374
                     /locus_tag="VIX62_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860421.1"
                     /translation="MSEGQGTHRRVGPGAVVVLGGLALAFLAAFLPLWYWSNAWIGSE
                     RVDPSLPVGMFDGAQLVDWWGVLGPNVAGRFHPVTSMLVTWVPVGVVLASAIVPLAVR
                     SWRGAFTPAVVVSVTMWGALVASWAALYRDNTDAWHLEPGAYVLGAGLAIALAGAIWD
                     RRRTAGHR"
     gene            1415..1678
                     /locus_tag="VIX62_09080"
     CDS             1415..1678
                     /locus_tag="VIX62_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860422.1"
                     /translation="MPDWLLVVTAILVGALIWRIGIASLRVFSPDPDEVPAPAEAEDV
                     EALEVYFVCGECGTELKVSRLGEMQVPRHCGEKMQVVRRPAGI"
     gene            complement(1904..2164)
                     /locus_tag="VIX62_09085"
     CDS             complement(1904..2164)
                     /locus_tag="VIX62_09085"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEY5860423.1"
                     /translation="MPVSKGRQKKRAPRYRAAPQQAKKRHRSSPRWYGPLLIGLMALG
                     VVVIVANYVGLIPGTDGNASSVWLWVGLGILGAGFVGTSFWY"
     gene            complement(2166..>3038)
                     /locus_tag="VIX62_09090"
     CDS             complement(2166..>3038)
                     /locus_tag="VIX62_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY5860424.1"
                     /translation="RMHDDGYPHLIGHDFHTPWRARRIAHVLGSHDGHDVAGMAALQV
                     DVMSLSARENVPLLLGRVEAAATHGSALAVLGSWDGAMTADSAATAIYQTWCGHIARL
                     WLVPRLGEELFGRYHAWREPFQCGALAGLLRDGALEGDLLGEALEAALAELRQRLGDD
                     AASWRWGDLHRVALAHPLAAIPGLEELFVALEAGVGGDDQTVLQTGVDARDGGFPAAV
                     IPSWRAVYDLGDLDRSVGVLPTGNSGNPASAHWNDQAALWLAGETHPLPFSRAAVETA
                     SVAMARLVPGSISG"
BASE COUNT          452 a         1050 c         1072 g          464 t
ORIGIN      
        1 gacccgaagc gaccgggtac ggcgccgtct acttcgccca ggagatgctc gcgaccaggg
       61 acgactcgct cgagggcaag acctgcctcg tgagcggggc cggcaacgtg gcccagtaca
      121 ccgtggagaa gctgctgcac ctgggggcca agccggtcac gctgtcggac tcgggcgggt
      181 tcatccacga ccccgacggc atcgacgagg agaagctcgc gtgggtgatg gacctcaaga
      241 acgaacgccg cgggcgcatc atggcgtacg ccgaccggtt cccggccgcc acgttcacgc
      301 ccgccgaccc gtccgacgac cacaacgcgc tgtgggaggt gccggccgac tgtgcgttcc
      361 cgagcgcgac ccagaacgag atcaacggca aggacgcggc gaacctgatc ggcggggggg
      421 tgcgggtcgt gagcgagggc gcgaacatgc cgaccgagcc cgagggggtc gaccagttcg
      481 tgacggcggg cgtgctttac ggacccggca aggcggcgaa cgcgggcggg gtcgcggtga
      541 gcggcctgga gatgtcgcag aacgcgatgc ggctgtcgtg gaccagggaa ggcgtcgacg
      601 agcgcctgcg cgagatcatg cggcgggtgc acgagcagtg ccgcgatacc gcggaggagt
      661 acggcgagcc cggcaactac gtgcacggtg cgaacatcgc ggggttccgc aaggtggcgg
      721 acgcgatgct cgcccaaggt gtgatctgat ccggcggtcc gctggcgttc ctgttgcggg
      781 acggtgtgaa ccgacccttc cctcttgccc gcgacacggc acctcggcac gctcatcgcg
      841 acccgaccac ggagtaagga gtgtgggatg agcgaaggcc agggaacgca ccggcgtgtg
      901 ggacctgggg ccgtggtcgt gctcggtggg ttggctctcg ccttcctggc ggccttcctc
      961 ccgctctggt actggagcaa cgcctggatc ggctcggaac gggtcgatcc gtcgttgccc
     1021 gtcgggatgt tcgacggcgc ccagttggtc gactggtggg gggttctcgg ccccaacgtg
     1081 gcggggcggt tccaccccgt cacgtccatg ctcgtgacct gggtaccggt cggcgtggtg
     1141 ctcgcgtcgg cgatcgtgcc cttggccgtt cgatcgtggc ggggggcgtt cacccctgcc
     1201 gtggtggtat cggtcacgat gtggggtgct ctggtcgcct cgtgggccgc cctctaccgt
     1261 gacaacacgg atgcgtggca tctcgagccg ggtgcttacg tgctcggcgc cggtctcgcc
     1321 atcgctctcg ccggggcgat atgggatcgg cgcaggacgg ctggacatcg gtgaaccggg
     1381 ccggccgtcg gagcgacgct gttagcgtgg aggcgtgccg gactggttgc tcgtcgtcac
     1441 cgcgatcctg gtcggtgcgc tgatatggcg catcggcatc gcgtcgttgc gcgtgttctc
     1501 gcccgacccc gatgaggtgc cggcgcccgc cgaggcagag gacgtcgagg cactcgaggt
     1561 gtacttcgtc tgcggggaat gtggcaccga actgaaggtg tcgcggctcg gcgagatgca
     1621 ggtgccgcgg cattgcggcg agaagatgca ggtggtgcgg cgcccggccg gcatctgagt
     1681 tgtccccagg gtttccaccg ctctcgggta gtgcgggtgg ggttttccac cgtttgcgca
     1741 ccaggctgtc cacgacggcg tgaccagcac atgaacgccg gttgacgacg caggtggcta
     1801 tccacaccgg tgggaaacgc tgtggaagga tcacaccggt gtgattcgtg gcccaggagc
     1861 agaccttggc gactcgcagc aagcgcttgc caacacccgc cgctcagtac cagaagctcg
     1921 tgcccacgaa gcccgctccc aggatcccca gccccaccca gagccacacc gacgacgcgt
     1981 tcccgtcggt tcccgggatc agtcccacgt agttcgccac gatcacgacc acgccgagcg
     2041 ccatcagccc gatgagcagc ggcccgtacc agcgcggact tgagcgatgg cgcttcttcg
     2101 cctgctgggg cgcggcccgg tagcgcgggg cgcgcttctt ctgtcgtccc ttggaaaccg
     2161 gcatctcagc cggagatgct acccggcacg agccgagcca tggcaaccga tgccgtctcg
     2221 accgccgccc gcgagaacgg cagcgggtgc gtttcgcccg ccagccacag cgccgcctgg
     2281 tcgttccagt gtgcgctcgc cgggttcccg ctgttgccgg tcggcaacac gcccacgctg
     2341 cgatcgaggt cccccaggtc gtacaccgcc cgccacgacg ggatcaccgc cgccgggaat
     2401 cccccatcgc gggcgtcgac ccccgtctgc agcacggtct ggtcgtcgcc gcccacgcct
     2461 gcttccagcg ccacgaacaa ctcctcgagc cccgggatcg ccgccagcgg gtgcgcgagc
     2521 gcgacccgat gcaggtcgcc ccatctccag ctcgcggcgt cgtcgccgag gcgctggcgc
     2581 aactcggcca gcgccgcttc gagcgcctcg ccgagcaggt cgccctccaa cgccccgtcg
     2641 cgcagcagcc cggccagtgc cccgcactgg aacggctcgc gccacgcgtg gtagcgcccg
     2701 aacagctcct cgccgagccg cggcaccagc cagaggcggg cgatgtgccc gcaccaggtc
     2761 tggtagatcg cggtggcggc cgagtcggcg gtcatcgcgc cgtcccatga acccagcacc
     2821 gcgagcgccg acccgtgagt cgctgccgct tcaactcgcc ccagcaacag cggcacgttc
     2881 tcgcgcgccg acagcgacat cacgtccacc tgtaaggccg ccatgccggc cacgtcgtgc
     2941 ccatcgtggg accccagcac gtgcgcgatg cgtcgcgccc gccacggcgt gtggaagtcg
     3001 tggccgatca ggtgcggata tccgtcgtcg tgcatgcg
//
LOCUS       DATDBZ010000381         3664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1768395, whole genome shotgun sequence.
ACCESSION   DATDBZ010000381 DATDBZ010000000
VERSION     DATDBZ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768395"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VIX62_09095"
     CDS             complement(<1..201)
                     /locus_tag="VIX62_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEY5860425.1"
                     /translation="MRRSERLSEILGILAADGAVDVTALAEGLGVSEATVRRDLRLLE
                     DQRMLARTHGGAVANDVMYELPL"
     gene            291..1184
                     /locus_tag="VIX62_09100"
     CDS             291..1184
                     /locus_tag="VIX62_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY5860426.1"
                     /translation="MSETAQEVASQPDRWRRAVALVPGVDPALPAVGERVAVVGCGTS
                     LYVARSYAGLREEAGQGETDAFPASEFPAGRSYDRVVAISRSGTTSEVVTLLRRLDRV
                     RTVVITGVPTSPAVEIASDAVTLEFADERSVVQTRFATSTLAMLRAHLGHELGPVIAD
                     AERALAEPVPAEDVDHDHFVFLGHGWSVGIAEEAALKLREGTRSYTEAYPAMEYRHGP
                     ISIAGTRTLVWVIGTPDPSIAEDVRAVGASVRAATFDPMAELVRVHRLTLALAERRGL
                     DPDHPQHLTRSVVLSAKGANP"
     gene            1181..2503
                     /locus_tag="VIX62_09105"
     CDS             1181..2503
                     /locus_tag="VIX62_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5860427.1"
                     /translation="MKRRFTRWLAAGAALMLLGAACTFGAEDTGDGGGTAAPDEPVTL
                     RVWSLQSPSYKRAFKDLEAGFEDANPNVDVEVEFFDYDTYIQTLQTSLPAGSGADVVQ
                     LFGTWVCSYADTLTSVPSDVMSLEDARADFYEAPIDGFTCDETLYGFPQEFNIEYGAT
                     LVNTEIAEASGVDPTAGWASWDEFKADAKAMTETRDGAITRAGYHFTAGDGLPFTFYS
                     LILQAGGHFLADDGQSFTIDTPEAREAVALMQSIVDEGITDPTLFNDTENWVGDCYFE
                     EVCSMGLVGPWVITDYEADFPEVAAATQYVPLPTLGETPDFVADSGWGLTVSADSQVT
                     NVAWDFVEYATLDPETAAEWNIATGTLPALRANGEGAVQDELIDAFPHFAPWFELLPY
                     GQFIGNLPDRDLLWYEITYPHLLKVLQGGESVDDALAAMESEANEMFR"
     gene            2515..3519
                     /locus_tag="VIX62_09110"
     CDS             2515..3519
                     /locus_tag="VIX62_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY5860428.1"
                     /translation="MASATIDRPRRRRRWLGGREGAQRRFVAVVLSPGLAYMAVFLLL
                     PIAWAILLAFFDFSPRRTGSPFLGLGLDNPYVGLEHFKRMFDFSNTAPLEVRQFHTAL
                     KVTLLFAFLVLPLNLAITLPLAAMIESVRDRWKVLYRTVFFLPVLTSAVGVAIIWGFV
                     LHPQRGLLNGTITRLTGRFTSIAWTTDPDLVFVGIPVALIAIIVAYLWQDIGYNLVIF
                     IAALQSIPDSVKDAARVDGASTWQTFRYITLPLLKPTILLVSVLTMISSFQVFDLFQV
                     MTNGGPDDQTRALSLDIYESAFRYQRMGWAAAVSVVLFLLVLTISLVQSRFLKPNWEY
                     "
BASE COUNT          567 a         1239 c         1231 g          627 t
ORIGIN      
        1 gagcggcagc tcgtacatca cgtcattcgc gacggcgccg ccgtgggtgc gcgccagcat
       61 cctctggtcc tcgagcaacc ggaggtctcg gcgcacggtg gcctcggaca cgccgaggcc
      121 ctctgcgagg gcggtgacgt cgacggcgcc gtcggccgca aggattccga ggatctcgct
      181 cagccgctcg gatcgtcgca tcgggcgaac tataagcctg gcccacgatt gcgcgctaga
      241 tgctcgaatc gattgaccac atgcacgaaa ctgctctact gtcccctgag atgagcgaaa
      301 ccgcgcaaga agttgcgtcg cagccggatc gctggcggcg ggcggtggcc ctcgtgccag
      361 gggtcgatcc cgcactaccg gctgtcggcg agcgggtcgc tgtcgtcggt tgcggcacgt
      421 cactctatgt ggctcggtcc tatgccgggc tgcgcgagga ggcggggcag ggggagaccg
      481 acgcgttccc cgcatcggaa ttcccggcgg gccgctccta cgaccgggtc gtggcgatca
      541 gccggagcgg cacgacgtcg gaagtcgtga ccctcctgcg tcggctggac cgggtgcgca
      601 cggtcgtgat caccggggtg ccgacctcgc ccgcggtgga gatcgcgtcc gacgccgtca
      661 ccctcgagtt cgcggacgag cgctccgtcg tgcagacgcg tttcgcgacg tcgacgctcg
      721 ctatgttgcg cgcccacctc ggccacgagc tcgggccggt gatcgccgac gcggagcgag
      781 cactcgccga gcctgtcccg gcggaggacg tcgaccacga tcacttcgtg ttcctcggac
      841 acgggtggag cgtcggcatc gccgaggaag cggcactcaa gctccgggag gggacgcgct
      901 cgtacaccga ggcatatccc gccatggaat accgacacgg cccgatcagc atcgccggca
      961 cacggacgct cgtgtgggtg atcggcaccc ccgatccctc gatcgccgaa gacgtgcgcg
     1021 ccgtaggtgc ctccgttcgc gcggcgactt tcgacccgat ggcggagctg gtgcgcgtac
     1081 accggctcac gctggccctc gccgagcggc gcgggctcga ccccgaccac ccgcagcacc
     1141 tgacccgatc cgtggtgctg tccgcgaaag gagcgaaccc atgaagcgtc ggttcacgcg
     1201 atggctggcg gccggggcgg cgttgatgct gctcggggcg gcgtgcacgt tcggcgccga
     1261 ggacacgggt gacggcgggg gaacggccgc gcccgatgag cccgtgacat tacgcgtgtg
     1321 gtcgctgcag agcccgtcat acaagcgagc cttcaaggat ctcgaggcgg gcttcgaaga
     1381 tgcgaatccc aacgtcgatg tggaggtcga gttcttcgac tacgacacct acatccagac
     1441 gctgcagacg tcgctgccgg ccgggagcgg cgccgacgtg gtccagctgt tcgggacgtg
     1501 ggtgtgcagc tacgccgaca ccctcacatc ggttccctcc gacgtgatgt cgctcgagga
     1561 cgcccgggcc gacttctacg aggcgccgat cgacggcttc acgtgcgacg agacgctgta
     1621 cgggttcccc caggagttca acatcgagta cggggctacg ctcgtgaaca ccgagatcgc
     1681 cgaagccagc ggcgtcgacc ccaccgcggg ctgggcctca tgggacgagt tcaaagccga
     1741 cgccaaggcg atgacggaga cgagggacgg cgcgatcacc cgagcgggct accacttcac
     1801 cgccggcgac ggcctgccgt tcacgttcta ctcgctcatc ctgcaggcgg gcgggcactt
     1861 cctggccgat gacggccagt cgttcacgat cgacacgccc gaagcccggg aggcagtcgc
     1921 cctcatgcaa tcgatcgtcg acgagggcat caccgacccg acgctgttca acgacacgga
     1981 gaactgggtc ggcgactgct acttcgagga agtgtgctcg atggggctgg tcggcccgtg
     2041 ggtgatcacg gactacgagg ccgacttccc ggaggttgcc gccgcgacac agtacgtgcc
     2101 cctcccgacc ctcggtgaga cccccgactt cgtcgccgac tcgggctggg ggctcacggt
     2161 ctcggcggac agtcaggtca cgaacgtcgc gtgggacttc gtcgagtacg cgacactcga
     2221 cccggagacc gccgctgagt ggaacatcgc caccggcacc ttgccggcgc tgcgagccaa
     2281 cggcgagggc gccgtgcagg acgaactgat cgacgccttc ccgcacttcg cgccgtggtt
     2341 cgagctgctt ccgtacgggc agttcatcgg gaacctcccc gaccgcgacc tgctctggta
     2401 cgagatcacg tatccgcacc tgttgaaggt gctccagggt ggcgagagtg tcgacgatgc
     2461 gctcgcggcg atggaatccg aagcgaacga gatgttccgc taggggctgc tttcatggcg
     2521 agtgccacga tcgaccgacc acggcgccga cgccggtggc tcggcgggcg tgaaggtgct
     2581 cagcgccggt tcgtcgcggt ggtcctctcg cccggactcg cgtacatggc ggtcttcctg
     2641 ctgcttccga tcgcctgggc gatcctcctg gcgttcttcg acttcagtcc tcgtcgcacg
     2701 gggagccctt tcctcgggct cgggctcgac aacccgtacg tgggcctcga gcacttcaag
     2761 cgcatgttcg acttctcgaa cacggcgccg ctcgaggtgc ggcagttcca caccgcgctc
     2821 aaggtcacgc tactgttcgc gttcctggtg ctgccgctga acctcgcgat caccctgccc
     2881 ctcgcggcca tgatcgagtc ggtgcgtgac cggtggaagg tgctctaccg caccgtgttc
     2941 ttcctaccgg tgctgacgtc ggcggtcggc gtcgcgatca tctgggggtt cgtgctgcac
     3001 cctcagcgcg gcctgctgaa cgggacgatc actcgcctca cgggcaggtt cacctccatc
     3061 gcgtggacga ccgacccaga cctcgtgttc gtcggcatcc cggtggcgct gatcgcgatc
     3121 atcgtcgcct atctctggca ggacatcggc tacaacctcg tgatcttcat cgccgcgctg
     3181 cagtcgatcc ccgactcggt gaaggatgcc gcgagggtcg acggcgcgag cacgtggcag
     3241 acgttccgct acatcaccct gccgctgctc aagccgacga tcctgttggt gtcggtgctc
     3301 acgatgatct cgtcgttcca ggtgttcgac ctgttccagg tgatgacgaa cggggggccc
     3361 gacgatcaga cgcgggcgct ctcgctcgac atctacgaga gcgcgttccg ctaccagcgc
     3421 atgggatggg ccgctgcagt ctctgtcgtg ttgttcctgc tggtgctcac gatctcgctc
     3481 gtgcagtcgc ggttcctcaa gccgaactgg gagtactagt ggccgcgacg atcgaacgac
     3541 cggtcgagga ggccacggca acgaccgggc gcgtgccacg cctgcccggc cagcgccgcc
     3601 gccggctcag ccgagcgtca gtgtacttct tgctgacggt cttcggggtc atcgcggcgg
     3661 tgcc
//
LOCUS       DATDBZ010000382         7036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_104169, whole genome shotgun sequence.
ACCESSION   DATDBZ010000382 DATDBZ010000000
VERSION     DATDBZ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104169"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /gene="aroC"
                     /locus_tag="VIX62_09115"
     CDS             <1..733
                     /gene="aroC"
                     /locus_tag="VIX62_09115"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017204367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY5860429.1"
                     /translation="AFLGELNVALLSHVVQIGTVKISKRRQLPTPDDLAAIDRSAVRC
                     FDPEASKQMVAEIDRLRKDRDTIGGVFEVLAYGAPPGLGSYVHYDRKLDARLALALMS
                     IQSVKGVEVGDGFATAAKPGSKAHDEIVPAHGRLTRATARAGGIEGGMSTGEPIRVRA
                     AMKPFSTVPKPLRTVDLATGEPAVAIKQRTDVCAVPAGGVVGEAVVGFELANAVLEKF
                     GGDSLGETADNLGRYLDGLTVEAKS"
     gene            730..1227
                     /locus_tag="VIX62_09120"
     CDS             730..1227
                     /locus_tag="VIX62_09120"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY5860430.1"
                     /translation="MIVYLVGMPGAGKTVVGRELAGKLGVPFVDLDAEIERDEGKPVS
                     EIFADEGEAAFRGMEAAALVKASASDPAVVSCGGGVVLEPANRITLRNTGTAVYLDVP
                     IDQLRERVRPGADRPLIREDGDLERLLEARGPLYREFAAHVVDGSGEPGEVADAIVEE
                     LRWSA"
     gene            1224..2297
                     /gene="aroB"
                     /locus_tag="VIX62_09125"
     CDS             1224..2297
                     /gene="aroB"
                     /locus_tag="VIX62_09125"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEY5860431.1"
                     /translation="MTVPVPGRAYDVVVGQGVIDEAASHLPEFPKAESAFVVADRDVA
                     DRWFDRLAAGLSGRGWSCTMLTVPAGEEAKSLDVYRVLLHQLATQEAHRDDLVVALGG
                     GSVGDLSGFVASTYMRGMPFVQVPTTLLAQVDAAIGGKTAVNLPEGKNLVGTFAQPRA
                     VLADVSTLATLPDRDFRSGLAEVAKYGLTLDTTLLDQLETDPAPVLARELDALETLVA
                     GCVRVKAHAVAEDERDTGARLILNYGHTLGHALERLEAFGGRSHGEAIAIGMVFAARL
                     AELRGLARSGLASRTVRLLSSLGLETDGSLPPVDDILAAFRLDKKYHGGVRFVLLRDV
                     GDAVVVDDVPEAQVREVLEAMGG"
     gene            2294..2728
                     /locus_tag="VIX62_09130"
     CDS             2294..2728
                     /locus_tag="VIX62_09130"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY5860432.1"
                     /translation="MKVLYLFGPNLGALGRRDPQTYGSETLEDIMASVTERGVELGHE
                     VAWRQSDHEGELVGWLLAAPSEGQVAVVINPGALTHYSYALRDAVEACELPVMEVHMS
                     NIYAREEFRRHSVVSEVCRATVTGLGAGGYHLALEAMPWITT"
     gene            2740..3789
                     /locus_tag="VIX62_09135"
     CDS             2740..3789
                     /locus_tag="VIX62_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5860433.1"
                     /translation="MAGRLPELAVDAFLVTGLTNVRYLTGFTGSNGQVLVAGGAGVFF
                     TDGRYTEQSRHEVPDLERVTYLGGFGEALGPQLAAMGIQRLGFEAHQVTVRTHEQLSA
                     KLAGVELVALGDEVERLRWVKDDEELENLRRAQAVTDQAFEDILETLAIGMTELAVAR
                     ELDNLLHRDGADGLSFESIVAFGENAAEPHHEPGHRLLDEGDVIKLDFGALYGGYHAD
                     MTRTVAFGEPATELKKVHDVVKQAQQAGIDAVAAGVTGQAVDVAARTVIEDAGYGQYF
                     THGLGHGVGLDIHEGPRLGREFASHVLPAGAVVTVEPGIYIPGLGGVRIEDMVEVTDD
                     GGRVLGTANRDLIEL"
     gene            3828..4394
                     /gene="efp"
                     /locus_tag="VIX62_09140"
     CDS             3828..4394
                     /gene="efp"
                     /locus_tag="VIX62_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY5860434.1"
                     /translation="MSVSTNDFKNGMTIDMDGTLFQIIEFQHVKPGKGGAFVRTKLRN
                     VKTGAVIERTFNAGVKVDQAIVERRGFQYLYPDGDEFVFMDLQTYDQIHVQRTLMGEA
                     SKFLVEGAEAQVATHDGVPISVDLPASLVLTITQTDPGVKGDTRTGSLKPATLETGVV
                     VNVPLFVEEGEKIKVDTRSGEYIERVKA"
     gene            4397..4843
                     /gene="nusB"
                     /locus_tag="VIX62_09145"
     CDS             4397..4843
                     /gene="nusB"
                     /locus_tag="VIX62_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY5860435.1"
                     /translation="MTTRREARRLAIDVLYQADITDRGPSDVLKGWREADRAIPAFTE
                     ELVDGVGEHGPEIDLLLEEHAQGWTVSRMTALDRTILRVAVEELLRGDVPPSVAISEA
                     VEAASELSAEESRSFVNGVLGRIAQETGTGASAEPIDVPDPGPAGA"
     gene            complement(4840..5088)
                     /gene="tatA"
                     /locus_tag="VIX62_09150"
     CDS             complement(4840..5088)
                     /gene="tatA"
                     /locus_tag="VIX62_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY5860436.1"
                     /translation="MGPPELIIVGIIAIAVIFGAKKLPEIAHNLGRSSNEFKKGLKEG
                     GEEADAPATQATPPPAQPIPPTQSVAPTETSDQPKPEA"
     gene            5375..5908
                     /gene="pyrR"
                     /locus_tag="VIX62_09155"
     CDS             5375..5908
                     /gene="pyrR"
                     /locus_tag="VIX62_09155"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY5860437.1"
                     /translation="MTGTLLDADDVRRALTRIAHEIIERDKGADGVVLVGIANRGDDL
                     ANRLAAEIERIEGTTVPVGALDITFYRDDIGMKAEAPEVHETRIGFDITGRTVVLVDD
                     VLFTGRTIRAAMDALMDFGRPRRIRLAVLVDRGHRELPIRADFVGKNVPTHRDDDVRV
                     LITELDGKDAVVVEETS"
     gene            5905..6837
                     /locus_tag="VIX62_09160"
     CDS             5905..6837
                     /locus_tag="VIX62_09160"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY5860438.1"
                     /translation="MNEHLLSMHDVSADDVVRILDTAESFREVGSRVIKKVPALRGRT
                     VVNFFLENSTRTRISFELAAKRLSADVINFSAGGSSVSKGESLKDTALTLQAMGADAI
                     VIRHSSSGSPLQLTKWVDGHVINAGDGTHEHPTQALLDLYTMREKLGRLEGLRVAIVG
                     DVLHSRVARSLSFGLVKMGAEVTLIGPPTLIPPDAGAWGVQVSYDIESVLPKLDVCYM
                     LRVQRERQRLEYFPSVREYNRIFGLSSKRAAMLPEGSVIMHPGPMNRGVEIDSDVADL
                     PQALIEEQVTNGIAIRMSLLYLLLGGAGEGALDG"
     gene            6830..>7036
                     /locus_tag="VIX62_09165"
     CDS             6830..>7036
                     /locus_tag="VIX62_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY5860439.1"
                     /translation="MADLLFTGARVIDPSTGMDEVTDVRVAGGLIEAVGNALDAAGAE
                     TLDGEGLILAPGLVDLHTHLREPGY"
BASE COUNT         1099 a         2283 c         2566 g         1088 t
ORIGIN      
        1 agcgttcctg ggtgagctca acgtggcgtt gctctcccat gtcgtgcaga tcggcacggt
       61 gaagatctcg aagcgccggc aactgccgac gcccgacgat ctcgccgcga tcgaccggtc
      121 ggcggtgcga tgcttcgatc ctgaggcctc caagcagatg gtggccgaga tcgaccgcct
      181 gcggaaggac cgcgacacga tcggcggggt cttcgaggtg ttggcctatg gcgccccgcc
      241 gggcttgggc tcctacgtgc actacgaccg caagctggac gcgcgtctgg ccctggcgct
      301 gatgagcatc cagtcggtga agggcgtgga ggtcggagac gggttcgcca cggctgcgaa
      361 gccgggatcg aaggcgcacg acgagatcgt gcccgcgcac ggccggctca cgcgcgccac
      421 ggcgcgcgcg gggggcatcg aaggcggcat gagcaccgga gagccgatcc gggtccgagc
      481 ggccatgaag ccgttctcca cggtcccgaa gccgctgcgg accgtggacc tggcgaccgg
      541 cgagcccgcg gtggccatca aacagcgcac cgacgtgtgt gcggtcccgg cgggcggggt
      601 cgtgggcgag gccgtcgtcg ggttcgaact cgcgaacgcg gtgctggaga agttcggagg
      661 cgattcgctc ggcgagacgg cggacaacct gggccggtac ctcgacgggc tgacggtgga
      721 ggcgaagtcg tgatcgtgta cctggtgggc atgcccggcg ccggcaagac ggtggtcggt
      781 cgtgagctgg ccggcaagct cggcgtaccg ttcgtcgatc tcgacgccga gatcgaacga
      841 gatgagggca agccggtgtc ggagatcttc gccgacgaag gcgaagccgc cttccgcggg
      901 atggaggcgg cggcgctggt gaaggccagc gcgtcggacc cagccgtggt ctcgtgcggc
      961 ggcggcgtcg tgctggagcc cgcgaaccgc atcaccctgc gcaacacggg cacggcggtc
     1021 tacctggacg tgccgatcga ccagctgcgc gagcgcgtgc ggcccggcgc cgaccgtccc
     1081 ctgatccgcg aggacggtga cctcgagcgc ctgctggagg cccgcggccc gctctatcgg
     1141 gagttcgccg cccacgtggt ggacggcagc ggggaacccg gtgaggtcgc cgacgcgatc
     1201 gtcgaggagc ttcgttggtc cgcgtgaccg taccggttcc cgggcgagcc tacgacgtgg
     1261 tcgtgggcca gggcgtgatc gacgaggcgg cctcgcacct tccggagttc ccgaaggccg
     1321 agagtgcctt cgtcgtggcc gatcgcgacg tcgccgaccg gtggttcgat cgcctggccg
     1381 ccggattgtc cggtcgcgga tggtcgtgca ccatgctgac cgtaccggcc ggcgaggagg
     1441 cgaagtcgct cgacgtctac cgtgtcctgc tgcaccagct ggcgacccag gaggcgcatc
     1501 gggacgacct cgtcgtggcc ctgggtggcg ggtcggtcgg cgacctgtcg gggttcgtcg
     1561 cctcgaccta catgcggggc atgcccttcg tgcaggtgcc gacgacgctc ctggcgcagg
     1621 tggatgccgc catcggtggg aagacggccg tgaacctgcc ggaaggcaag aacctggtgg
     1681 ggacgttcgc gcagccgcgc gccgtgctcg ccgacgtctc gaccctggcc accttgcccg
     1741 atcgggactt ccgctccggg ctggcggagg tggcgaaata cgggctcacg ctcgatacga
     1801 cactgctcga ccagctggag accgatcccg ccccggtgct cgcacgtgag ctcgatgccc
     1861 tcgagaccct cgtcgccggg tgcgtccgcg tgaaagcgca tgcggtcgcc gaggacgaac
     1921 gcgacacggg cgcccgcctg atcctcaact acggtcacac cctgggtcat gcgctcgaac
     1981 ggctcgaggc gttcggcggg cgctcgcacg gggaggcgat cgcgatcggg atggtgttcg
     2041 ccgcgcggct cgcggagctg cgcggcctcg cacggtcggg cctcgcctcg cgcacggtgc
     2101 ggctgctctc gagcctggga ctggagaccg atgggtcgct cccgccggtc gatgacatcc
     2161 tggcggcgtt ccgattggac aagaagtatc acggcggtgt ccgcttcgtg ttgctgcgcg
     2221 atgtcggcga cgccgtggtc gtggacgacg tccctgaggc gcaggtccgt gaggtactgg
     2281 aggcgatggg cggatgaagg tgctgtacct gttcggaccg aacctcggcg ccttgggccg
     2341 acgcgatccg cagacctatg ggtccgagac cctcgaggac atcatggcgt cggtcaccga
     2401 acgtggggtc gagctggggc acgaggtcgc ctggcgccag tccgatcacg aaggtgagct
     2461 cgtgggctgg ctcctggcgg cgccgtccga aggccaggtc gcggtcgtga tcaatccggg
     2521 ggcattgacc cactattcct acgccctgcg cgacgccgtc gaggcgtgcg agctcccggt
     2581 gatggaagtg cacatgtcca acatctacgc ccgcgaggag ttccgacgcc actcggtggt
     2641 ctcggaggtc tgccgggcca ccgtcaccgg cctcggagcg ggtggctatc atctggcact
     2701 ggaggcgatg ccgtggatca ccacttgagg aggaccgcga tggccgggcg actgcccgag
     2761 ctcgcggtcg atgcgttcct ggtcaccggg ttgacgaacg tccgctacct caccggcttc
     2821 accgggtcca acgggcaggt gctcgtggcc ggcggggccg gtgtcttctt cacggacggc
     2881 cgctacaccg agcaatcacg ccacgaggtg cccgacctcg agcgcgtcac ctacctcggc
     2941 gggttcggcg aggcgctcgg accgcagctg gcggccatgg gcatccagcg gctgggcttc
     3001 gaggcgcatc aggtcaccgt gcgcacgcac gaacagctct cggccaagct cgccggcgtg
     3061 gagctcgtgg cgctgggtga cgaggtggaa cggctgcggt gggtgaagga cgacgaggag
     3121 ctcgagaacc tccgtcgggc ccaggcggtc accgaccagg cgttcgagga catcctcgag
     3181 accctcgcga tcggcatgac cgagctggcc gtggcccgtg agctggataa cctgctgcat
     3241 cgtgacggcg ccgacggcct cagcttcgag tcgatcgtgg ccttcggcga gaacgccgcc
     3301 gagccgcacc acgagcccgg acaccgcctg ctcgacgaag gcgacgtcat caagctggat
     3361 ttcggcgcgc tgtacggcgg ttatcacgcc gacatgacgc ggaccgtggc gttcggcgag
     3421 cccgccaccg agctcaagaa ggtgcacgac gtcgtgaagc aggcccagca ggccggtatc
     3481 gacgccgtgg cggccggggt caccggccag gccgtcgatg tcgcggcccg caccgtgatc
     3541 gaggacgccg gctacggcca gtacttcacg cacgggctcg gacacggcgt cggcctggac
     3601 atccacgagg ggccgcggct cggtcgcgag ttcgcctcgc acgtgttgcc ggcgggggcc
     3661 gtggtgaccg tggaaccggg gatctacatc ccgggcctcg gcggggtgcg catcgaggat
     3721 atggtggagg tcaccgacga cggcggccgc gtgctcggca ccgccaatcg agatctgatc
     3781 gagctgtaac gaccaccgtt ccgacgaccg aggagctgaa cccccgcgtg agcgtttcca
     3841 ccaacgactt caagaacggc atgacgatcg acatggacgg caccctgttc cagatcatcg
     3901 agttccaaca cgtgaaaccc ggcaagggcg gtgcgttcgt gcggacgaag ctgcgcaacg
     3961 tgaagaccgg tgccgtgatc gagcgcacgt tcaacgccgg cgtcaaggtg gatcaggcga
     4021 tcgtggagcg tcgcgggttc cagtacctgt atcccgacgg cgacgagttc gtgttcatgg
     4081 acctgcagac ctacgaccag atccacgtgc agcgcaccct catgggcgag gcgtccaagt
     4141 tcctggtgga gggggccgag gcgcaggtcg ccacgcacga cggggtcccg atctcggtcg
     4201 acctgcctgc atcgctggtg ctcacgatca cccagaccga ccccggggtg aaaggcgaca
     4261 cgcgcacggg ttccctcaag ccggccaccc tggagaccgg cgtggtcgtc aacgtgccgt
     4321 tgttcgtcga agagggcgag aagatcaagg tcgacacgcg ttcgggcgag tacatcgaac
     4381 gcgtgaaggc ctgagcgtga ccaccaggcg cgaagcgcga cgcctggcga tcgacgtcct
     4441 gtaccaggcc gacatcaccg atcgcggtcc ctccgacgtg ctgaaaggtt ggcgtgaggc
     4501 cgatcgtgcg atcccggcgt tcaccgagga gctggtggac ggagtggggg agcacggtcc
     4561 cgagatcgat ctgctgctgg aggagcatgc ccagggttgg acggtctcgc gcatgacggc
     4621 gctggaccga accatcctgc gggtggcggt ggaggagttg cttcgggggg acgtgccgcc
     4681 ttcggtggcg atcagcgaag ccgtggaagc ggcctcggaa ctgtcggccg aggagtcacg
     4741 ctcgttcgtg aacggcgtgc tgggccggat cgcgcaggag accgggaccg gggcgagcgc
     4801 cgaaccgatc gacgtgccgg acccgggtcc agccggggcc taagcctcgg gcttgggctg
     4861 atccgaggtc tcggtcgggg ccacggactg cgtgggcggg atcggctgag cggggggcgg
     4921 ggtcgcctgg gtggcggggg cgtctgcctc ttccccgccc tccttgaggc ccttcttgaa
     4981 ctcgttggag gagcgaccca ggttgtgcgc gatctcgggc agcttcttgg caccgaagat
     5041 cacggcgatc gcgatgatcc caacgatgat cagctcggga ggacccatgg gcagtggccg
     5101 cctttcggat tcggggatgt cttcggtggt gctgtgcggc cagtgtacgc ccgccgatgg
     5161 tctgcggcca taaagggagg cgccggggga tgtgacgagc ggggctcgtt gcgaccacac
     5221 acacgggacc gctatgctcg gaggcgacag cgcttccccc tttaagtccg gtcccgcgag
     5281 accgggaagg gaacatgaac atgacgcaca acccatgcgc ctgcccagca ccctgggcgg
     5341 gcgttcctgt ttccgcggtc gcgccggtgt ccggatgacg ggcacgttgc tcgacgccga
     5401 cgacgttcgc cgcgccctca cccgtatcgc ccacgagatc atcgaacgtg acaagggtgc
     5461 cgacggcgtg gtgctcgtgg ggatcgcgaa ccgcggcgac gacctcgcga accgactcgc
     5521 cgccgagatc gagcgcatcg agggcaccac ggtgccggtg ggggcgctcg acatcacctt
     5581 ctaccgcgac gacatcggga tgaaggccga ggctccggag gtgcacgaga cgcggatcgg
     5641 cttcgacatc accgggcgca ccgttgtcct ggtcgacgac gtgctgttca cgggccgcac
     5701 gatccgcgcg gccatggacg cgctgatgga cttcggccgg ccccgcagga tccgcctcgc
     5761 cgtgctggtc gaccgcggcc accgcgagct gccgatccgg gctgacttcg tgggcaagaa
     5821 cgtgccgacg caccgcgacg acgacgtccg ggtgctgatc accgagctgg acggcaagga
     5881 cgccgtggtg gtggaggaga cctcatgaac gaacatctgc tctcgatgca cgacgtcagc
     5941 gccgacgacg tcgtccgcat cctcgacacg gcggagtcct tccgagaggt cggctccagg
     6001 gtcatcaaga aggtccccgc gctgcggggc cgcacggtcg tgaacttctt cttggagaac
     6061 tccacccgca cgcggatcag cttcgagctg gcggccaagc ggttgtcggc ggacgtgatc
     6121 aacttcagcg ccggcgggtc gagcgtctcc aagggcgaga gcctgaagga caccgctctg
     6181 acgctgcagg cgatgggtgc cgacgcgatc gtgatccggc actcttcgag cggctcgccg
     6241 ttgcagctca cgaagtgggt ggacggccac gtgatcaacg ccggcgacgg cacgcacgag
     6301 caccccacgc aggcgctgct cgacctctac acgatgcgcg agaagctggg gcgcctcgag
     6361 ggcctgcggg tcgcgatcgt gggtgacgtg ctgcactcgc gggtggcgcg ctcgctttcg
     6421 ttcgggttgg tgaagatggg cgccgaggtc acgctgatcg gcccgccgac gctgatcccg
     6481 cccgacgccg gcgcctgggg cgtgcaggtg agctacgaca tcgagagcgt gctgcccaag
     6541 ttggacgtct gctacatgct ccgcgtccag cgcgagcgcc agcgcctcga gtacttcccc
     6601 agtgtgcggg agtacaaccg gatcttcggg ctgagttcga aacgggcggc catgctccct
     6661 gagggctcgg tcatcatgca ccccggcccc atgaaccgag gcgtcgagat cgactccgac
     6721 gtcgccgacc tgccccaggc gctgatcgag gaacaggtca cgaacgggat cgcgatccgc
     6781 atgtcgctgc tctacctctt gctcggcggc gccggagagg gggccctcga tggctgacct
     6841 gctgttcacg ggcgcgcggg tgatcgaccc ctcgaccggc atggacgagg tcaccgacgt
     6901 ccgggtggcc ggcggcctga tcgaggccgt cgggaacgcc ttggacgccg ccggcgccga
     6961 gacgctcgat ggtgagggac tgatcctggc cccgggcctc gtcgacctgc acacgcacct
     7021 gcgtgagccg ggctac
//
LOCUS       DATDBZ010000383         4086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1133815, whole genome shotgun sequence.
ACCESSION   DATDBZ010000383 DATDBZ010000000
VERSION     DATDBZ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133815"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VIX62_09170"
     CDS             <1..337
                     /locus_tag="VIX62_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5860440.1"
                     /translation="ILPYCVSKGALAVMTKNAAYSLMRHHIRVNQVNPGWMDTEAEDA
                     VQRRFHGATDGWLEAAEAEQPFGRLIKPPEVAKVIAFYLSDESGMMTGTIVDYDQSVQ
                     GAGDAPKPY"
     gene            503..1510
                     /gene="iolG"
                     /locus_tag="VIX62_09175"
     CDS             503..1510
                     /gene="iolG"
                     /locus_tag="VIX62_09175"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEY5860441.1"
                     /translation="MTVRVAVMGVGRIGRMHAELLAHRVDGAELAMVQDVNRAAAASI
                     GAALGVPFTTDPGAVLAADDVDAVAICSSTDTHAELLTLAASAGKAIFCEKPVSLDLA
                     QLDRALAAVAAAGLPLQIGFNRRFDPAHRSVRDAVQRGDLGDLHLVRITSRDPSPPPI
                     EYVKVSGGIFLDMMIHDFDMARYVTDSEVVEVYAQGAVRVDPEIGNAGDVDTAVVVLR
                     HENGCLTTIDNSRRAVYGYDQRVEAFGPGGMAASENPLAHSGIRRTATGTSAQLLPFF
                     FLDRYIPSYVHQWDAFVAYVRDGGDSPVSAHDGRAPLVIGLAAWRSVREGRPIATSEI
                     G"
     gene            complement(1636..2154)
                     /locus_tag="VIX62_09180"
     CDS             complement(1636..2154)
                     /locus_tag="VIX62_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY5860442.1"
                     /translation="MAPTPREGCIHVVIEIPRGSRNKYEIDHDTGRIFLDRRLFTATT
                     YPADYGFVPDTLSGDGDPLDALVLLEDPVYPGVWVEARPVGLLWMQDEAGPDAKLICV
                     PSKEPRWKGVQDLKDLPEELLEEIKHFFDVYKALEPDKYAATAGFENRKAAWREIESG
                     KANFRASGAHPH"
     gene            2249..3451
                     /locus_tag="VIX62_09185"
     CDS             2249..3451
                     /locus_tag="VIX62_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5860443.1"
                     /translation="MEFRRITNLPPYVFTIINGLKIEARRAGADVIDLGFGNPDIPSP
                     EIAVEKLAEAARIPRNHRYSLSRGLPKLREAAAALYERKWGVHLDPELEITNTIGSKE
                     GFSHLMWVLLEQGDAAIVPSPSYPIHIYGPLFAGADLRQVPMRSLSDPKVQEDFTGDF
                     FDSLENAWNVGWPKPRVLVISFPHNPTGSCVDLDFMQRVVDFCREREMIVVHDFAYAD
                     VGFDGYEPPSILQAEGARECAVELYSMTKSFSMAGWRCAFLVGNQDVVQALVKLKSYL
                     DYGMFQPVQIAATVTMNEAPDFPKEVCSIYQGRRDILIDGLSRIGWDIPKPKGTMFVW
                     APIPEPYKDLDSVEFCSLMVRECDVALSPGLGFGPGGEGFVRFALIENEKRIAQGIRN
                     LKRGLTKL"
     gene            3561..>4086
                     /locus_tag="VIX62_09190"
     CDS             3561..>4086
                     /locus_tag="VIX62_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860444.1"
                     /translation="MLTYVVRVWVPDRPGALGQVASRIGAVHGDVVGIDILERGAGMA
                     IDELVVTLASADLVDLLVTEIGEVDGVAVEEIRPVPAGRPESAIVALQIAARLVELEQ
                     HKLLDGLCVETLQFLEADWVVALSPSEEQVHVELGRAPAPGWLGAFIVGSRHLEPAQQ
                     HETAPHDLAWASLDG"
BASE COUNT          636 a         1371 c         1360 g          719 t
ORIGIN      
        1 catcctcccg tactgcgtgt ccaagggcgc gctcgccgtg atgacgaaga acgccgccta
       61 ctcgctcatg cggcaccaca tccgggtgaa ccaggtgaac cccggctgga tggacacgga
      121 agcggaggat gcggtgcagc ggcgcttcca cggcgcgacc gacggatggc tcgaggcggc
      181 ggaagccgag cagccgttcg gccggctgat caaaccgccg gaagtggcga aggtgatcgc
      241 cttctatctc tccgacgagt ccgggatgat gaccggcacc atcgtcgact acgaccaatc
      301 cgtccagggc gccggcgacg cccccaaacc gtactgatgg cctcgactgg cctccgcttc
      361 gcttcggctg ctctcggcgg cggctcgtcg ctggcgcttc cttgctgatg atctcgttcg
      421 atctccgctc cgctttgatc tcactcgatt gcggctcgtc gctggcgctt ccctcgccgc
      481 gttcttactt ctggaggtgc tcatgacggt ccgggttgcg gtgatggggg tggggcggat
      541 cgggcggatg cacgccgagt tgctcgcgca ccgggtcgac ggggccgagt tggccatggt
      601 gcaggacgtg aaccgggcgg cggcggcatc gatcggcgct gcccttggcg tgccgttcac
      661 gaccgacccc ggcgcggtgc tggccgcgga cgacgtggat gcggtcgcga tctgctcgtc
      721 aaccgacacc catgcggagc tcctgacgct ggcggcgtcg gccggcaaag cgatcttctg
      781 cgagaaaccg gtgtcgctcg acctcgccca gctcgaccga gcgctcgctg ctgttgccgc
      841 cgctggtcta cccctccaga tcgggttcaa ccggcgattc gatcccgccc atcgctccgt
      901 gcgggacgcc gtccaacgcg gagatctcgg cgatctccac ctggtgcgga tcaccagccg
      961 cgatccgtcc ccgcccccga tcgagtacgt gaaggtgtcc ggcgggatct tcctcgacat
     1021 gatgatccac gacttcgaca tggcccgtta cgtcaccgac agcgaggtgg tcgaggtcta
     1081 cgcacagggg gcggtgcggg tcgatccgga gatcggcaat gccggtgacg tggacacggc
     1141 cgtggtcgtg ctccgccacg agaacggctg cctcaccacg atcgacaaca gccgccgggc
     1201 ggtgtacggc tacgaccagc gggtcgaggc gttcggcccc ggggggatgg cggcgtcgga
     1261 gaacccgctc gcccattccg gcatccggcg caccgcgacc ggcacctcgg cccagttgct
     1321 gccgttcttc ttcctcgacc gctacatccc gtcgtacgtc caccagtggg acgcgttcgt
     1381 ggcctacgtg cgcgacggcg gcgattcccc cgtctccgcc cacgacggca gggcaccgct
     1441 cgtcatcggg ctggcggcgt ggcgatcagt ccgcgagggt cgcccgatcg cgacctccga
     1501 gatcggctag accaccgacc gctacctgtc agcgtccccg atacctgtca gcgattctcc
     1561 gccagggcgc gagatcgctg acatggatca acgtggctga cacgaaaggg ctaggagtca
     1621 accacggctg gcgggtcagt gcggatgcgc gccgctggcg cggaagttcg ccttgcccga
     1681 ctcgatctcc cgccaggcgg ccttgcggtt ctcgaacccg gccgtggcgg cgtacttgtc
     1741 cggttcgagt gccttgtaga cgtcgaagaa gtgcttgatc tcctcgagca actcctcggg
     1801 gagatccttc agatcctgga cgcccttcca acggggttcc ttcgacggca cgcagatgag
     1861 cttggcgtca ggtccggcct cgtcctgcat ccagaggagc ccgaccggcc gcgcctcgac
     1921 ccacacaccg gggtagaccg ggtcctccag caggaccagc gcatcgagcg ggtcgccgtc
     1981 gccgctcagc gtgtccggca cgaatccgta gtcggcggga taggtcgtcg cagtgaaaag
     2041 gcgccggtcg aggaagatcc gcccggtgtc gtggtcgatc tcgtacttgt tcctgcttcc
     2101 ccgggggatc tcgatcacga cgtggatgca tccctcgcgg ggggtgggtg ccatggccgg
     2161 gaatgctggc acaggaggag acgatcgcgc tgctcatgac gggaaccgcc tgggctgctc
     2221 gggtgcgaac ggttagcgtc gggcgcctat ggagttccgt cggatcacga acctgccgcc
     2281 ctatgtcttc accatcatca atggcctgaa gatcgaggca cggcgagcag gcgccgacgt
     2341 gatcgacctc ggcttcggca accccgacat cccctcgccc gagatcgcgg tcgagaagct
     2401 ggccgaggcg gcgcgcatcc cgcggaacca tcgctactcg ctctcgcgcg gcttgccgaa
     2461 actccgcgag gccgccgccg cgttgtacga gcgcaagtgg ggtgtccatc tcgaccctga
     2521 gctcgagatc accaacacga tcgggtccaa ggaaggcttc tcccacctca tgtgggtgct
     2581 gctcgagcag ggcgacgccg ccatcgtgcc gagcccgagc tacccgatcc acatctacgg
     2641 gccgctgttc gccggcgctg acctgcggca ggtgccgatg cgctcgctgt ccgatccgaa
     2701 ggtccaggag gacttcaccg gggacttctt cgacagcctc gagaacgcct ggaacgtcgg
     2761 atggccgaag ccgcgagtgc tcgtgatctc cttcccgcac aacccgaccg gatcgtgcgt
     2821 cgacctcgac ttcatgcaac gtgtcgtcga cttctgccgt gagcgggaga tgatcgtcgt
     2881 gcacgacttc gcctatgccg acgtcgggtt cgatgggtac gagccaccgt cgatcctcca
     2941 ggcggagggg gcgagggagt gtgcggtcga gctctactcg atgacgaaga gcttctccat
     3001 ggccggttgg cggtgtgcgt tcctcgtcgg caaccaggac gtcgtgcagg cactcgtcaa
     3061 gctcaagagc tatctcgact acggcatgtt ccagcccgtg cagatcgccg ccacggtgac
     3121 gatgaacgaa gcaccggact tcccgaagga ggtctgttcg atctaccagg gtcgccggga
     3181 catcctcatc gacgggttgt cgcgcatcgg atgggacatc ccgaagccga aaggcacgat
     3241 gttcgtctgg gcgccgatcc ccgagccgta caaggacctc gactcggtcg agttctgctc
     3301 cctgatggtg cgcgagtgcg acgtcgcact gtcgcccggg ctcggcttcg gccccggcgg
     3361 cgaaggcttt gtgcgcttcg cgctgatcga gaacgagaag cgcatcgcgc aaggcatccg
     3421 caacctcaag cggggactca cgaagctctg accgtgcggc cccgtcggcc gccgtcgggt
     3481 gccgtccggg atcgagcgtt cacacggtcg tcaagatccc gtcatcttgc gcgcaatatg
     3541 cgtcgatacc gtccggttcc gtgctgacct acgtggttcg ggtctgggta cccgaccgtc
     3601 cgggtgcgct cgggcaggta gcgagccgga tcggcgctgt gcacggcgat gtggtgggca
     3661 tcgacatcct ggaacgcggc gccggcatgg cgatcgacga gctcgtcgtg accctggcct
     3721 ccgcggatct cgtcgacctg ttggtcaccg agatcggcga ggtggacggt gtcgccgtcg
     3781 aggagatccg cccggttcct gccggtcgcc cggagagcgc gatcgtcgca ctgcagatcg
     3841 ccgctcgcct ggtggaactc gaacaacaca agctcctgga tggcctctgt gtcgaaaccc
     3901 tgcagttcct ggaggccgat tgggtggtcg cactgtcacc ctccgaggag caggtgcatg
     3961 tcgagctcgg ccgggcgccg gcacccggat ggctcggtgc cttcatcgtc gggagtcgcc
     4021 atctcgagcc ggcgcaacag cacgagacgg caccgcacga tctcgcgtgg gcctcgctcg
     4081 acggtc
//
LOCUS       DATDBZ010000384         3145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1566651, whole genome shotgun sequence.
ACCESSION   DATDBZ010000384 DATDBZ010000000
VERSION     DATDBZ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566651"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2751
                     /locus_tag="VIX62_09195"
     CDS             <1..2751
                     /locus_tag="VIX62_09195"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY5860445.1"
                     /translation="YRNDAMAAHGEIAAKLVHLFEARFDPQLDASEEAIDEVRQEIHQ
                     DLRQVTSLDQDNILRSLLGTVEAIVRTNAYLVDRPALSFKLRSARVPEMPKPYPLFEI
                     FVYSPQMEGIHLRGGMVARGGIRWSDRKEDYRTEVLGLMKAQKVKNAIIVPDGSKGGF
                     ILKRATATPDELRAEVSTQYVTLMRGMLDITDNRVDGEVVHPAGVRIHDAPDPYLVVA
                     ADKGTATFSDTANAISEEYGFWLGDAFASGGSHGYDHKELGITARGAWESVKRHFREV
                     GIDVMAQPFTVVGIGDMSGDVFGNGMLYTPQIQLVCAFDHRHVFIDPTPDPAVSFAER
                     QRLFAIPRSSWADYDASLLSEGGAIVDRSTKSVTLSPQARAALGIPDDAPEEMSTNEV
                     IHRALQAPVDLLWNGGIGTYVKADGEGHTEVGDRANDPVRVNGNQVRARIVGEGGNLG
                     FTQRGRVEYAKQGGRINTDFIDNSAGVDTSDHEVNIKILLDLAVQRGELSMDERNVLL
                     QACAQDVVAHVLYDNYLQAQILSQEMEFSVQRIESYEELMQQLEAEGELEREVEFLPS
                     PDTMGERRGAGDGLVRPELAVLLAYAKRSIAAALLDSEFPDSPYLFQDLQSYFPPAVV
                     ERYGHLIEDHPLKRELVATIASNDVVNAQGITFVSRMVTETGASPADVVKAFRIARDV
                     TGSIQRWADIEALDGQIDPELQGELLNGVDWLVETTSRWYLVRAEGQRMSEAVDQARD
                     SFAELSDVIDQIGPEAWREEHEHEARRLVAEGVPPVLARRHAFQSELVHGPDIIAVAH
                     ATGRSVLEVARGFFLLGERLEIDWLESQLETMSVGTRWQRWAQQSMEDDLFNLRRQLA
                     EVALAECGPLPIDEAVEKFLESRAEPLGRLQRFMRGLAVDGMSDLAQLTVALRQIRAL
                     VG"
     gene            2791..>3145
                     /locus_tag="VIX62_09200"
     CDS             2791..>3145
                     /locus_tag="VIX62_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860446.1"
                     /translation="MRELEPPVDREPVERDSSSAMTSVDEPGEGDTTSAMDLAEGAVE
                     SFRTPVPVSRTPGSASGTPRTPRDGLPDYVALITAEIEAIEARRAAAARISEAVSPGT
                     PRPFSAGPSRKRYATA"
BASE COUNT          529 a          985 c         1138 g          493 t
ORIGIN      
        1 taccgcaacg acgccatggc cgcgcacggc gagatcgccg ccaagctcgt gcatctgttc
       61 gaggcgaggt tcgatcccca actcgatgcg agcgaggaag cgatcgacga ggtccgtcag
      121 gagatccacc aggacctgcg ccaggtgacc tccctcgacc aggacaacat cctgcgcagc
      181 ttgctgggca cggtcgaagc gatcgtccgc acgaacgcct atctcgtgga tcggccggcc
      241 ctcagcttca agcttcgttc ggctcgcgtg ccggagatgc ccaagcccta tcccttgttc
      301 gagatcttcg tgtactcacc gcagatggag ggcatccacc tgcgcggcgg catggtggcg
      361 cgcggcggga tccgctggtc ggaccgcaag gaggactacc gcaccgaggt cctcggcctg
      421 atgaaggccc agaaggtgaa gaacgcgatc atcgtgcccg acggttccaa gggcggcttc
      481 atcttgaagc gagccacggc cacgcccgac gagctccgtg cggaggtctc gacgcagtac
      541 gtcacgttga tgcgcgggat gctcgacatc accgacaatc gcgtcgacgg cgaggtggtg
      601 cacccggcgg gggtccgcat ccacgatgcg cccgacccgt acctggtggt ggcggccgac
      661 aagggcaccg ccaccttcag cgacaccgcg aacgcgatca gcgaggagta cgggttctgg
      721 ctcggggacg cgttcgcctc gggcggctcc cacggatacg accacaagga gctggggatc
      781 acggcccgcg gcgcgtggga gtcggtgaag cgccatttcc gcgaggtggg gatcgacgtc
      841 atggcccagc cgttcaccgt ggtggggatc ggggacatgt cgggggacgt gttcggcaac
      901 gggatgctct acacgccgca gatccagctc gtgtgcgcgt tcgaccaccg gcacgtgttc
      961 atcgatccca cgccggatcc cgccgtctcg ttcgcggagc ggcagcggct cttcgcgatc
     1021 cctcggtcct cgtgggccga ttacgacgcg tcgctcttga gcgagggcgg agcgatcgtc
     1081 gaccggtcga cgaagagcgt gacgttgtcg ccgcaggcac gtgcggcgct cggcatcccc
     1141 gacgacgctc ccgaggagat gtccaccaac gaggtgatcc atcgggcgct gcaggccccg
     1201 gtcgatctgc tctggaacgg tggcatcggc acgtacgtca aagccgacgg cgagggccac
     1261 accgaggtgg gcgaccgcgc gaacgacccg gtccgcgtca acggcaacca ggtgcgggca
     1321 cgcatcgtgg gcgagggcgg gaacctcggc ttcacccagc gaggccgggt ggagtacgcg
     1381 aagcagggcg gtcgcatcaa caccgacttc atcgacaatt cggcgggcgt cgacacctcc
     1441 gaccacgagg tgaacatcaa gatcctgctt gacctggccg tgcagcgcgg tgagctctcg
     1501 atggacgagc gcaacgtgct gttgcaggcg tgcgcgcagg acgtcgtggc ccacgtgctc
     1561 tacgacaact acctgcaggc ccagatcctg tcccaggaga tggagttcag cgtccagcgg
     1621 atcgagagct acgaggagct gatgcagcag ctcgaggccg aaggcgagtt ggagcgcgag
     1681 gtcgagttcc tgccgtcccc cgacaccatg ggagagcggc ggggggcggg cgatgggctg
     1741 gtgcgtcccg aactcgcggt gctgctcgcg tacgcgaagc gctcgatcgc cgcggcgctg
     1801 ctggactcgg agttccccga ctcgccctac ctgttccagg atctgcagtc ctacttcccg
     1861 ccggcggtgg tggagcgata cggccacctg atcgaggacc atccgctgaa gcgcgagctc
     1921 gtggctacga tcgcctcgaa cgatgtcgtc aacgcgcaag ggatcacgtt cgtctcgcgg
     1981 atggtgaccg agacgggcgc cagcccggcc gacgtggtga aggcgttccg gatcgcgcgg
     2041 gatgtgacgg gctcgatcca acggtgggcc gatatcgagg cgctcgacgg ccagatcgac
     2101 ccggaactgc agggcgagct gctcaacggt gtggactggc tggtggagac cacctcgcgc
     2161 tggtacctgg tgcgggccga agggcagcgg atgagcgagg cggtggacca ggcgcgggat
     2221 tcgttcgcgg agctgagcga cgtgatcgac cagatcgggc ccgaagcctg gcgagaggag
     2281 cacgagcacg aggcgcgccg gctggtcgcc gaaggggtac cgcccgtgct ggctcgacgc
     2341 cacgccttcc agtccgaact cgtgcacggc cccgacatca tcgcggtggc gcacgcgacg
     2401 ggtcgttccg tgctcgaggt ggcgcgaggg ttcttcctgc tgggggagcg gctcgagatc
     2461 gactggctgg aatcgcagct ggagacgatg tcggtgggca cgcgctggca gcgatgggcg
     2521 cagcagtcga tggaggacga tctgttcaac ctgcgtcgcc agctggcgga ggtggcgctg
     2581 gccgagtgcg ggccgttgcc gatcgacgag gctgtggaga agttcctgga atcgcgggcg
     2641 gagccgttgg gccggctgca gcggttcatg cgggggttgg cggtggacgg gatgagcgac
     2701 ctggcgcagc tgacggtggc gttgcggcag atccgggcgc tggtcggctg aaccggcagt
     2761 gtcagcgggc cggcgtatcg ttcgccgccg atgcgtgagc ttgaaccacc ggtcgaccgc
     2821 gagcccgtcg agcgcgacag cagttctgcg atgacctcgg ttgatgagcc tggcgagggg
     2881 gataccactt ccgcgatgga cctggcggaa ggggctgtgg agtcgtttcg tacaccggtt
     2941 ccggtatcgc gcacgccagg ttcggcatcg gggacgccgc ggacgcctcg ggatgggctt
     3001 cctgactacg tcgcattgat caccgcagag atcgaggcga tcgaagcccg tcgtgcagcc
     3061 gcggcacgga tatcagaagc ggtatcccca gggactcctc gacccttcag cgccggtcca
     3121 tcccggaagc ggtatgcgac cgcat
//
LOCUS       DATDBZ010000385         2462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2987336, whole genome shotgun sequence.
ACCESSION   DATDBZ010000385 DATDBZ010000000
VERSION     DATDBZ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987336"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2462
                     /gene="metH"
                     /locus_tag="VIX62_09205"
     CDS             <1..>2462
                     /gene="metH"
                     /locus_tag="VIX62_09205"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY5860447.1"
                     /translation="CSLGASEMRPYVEAFSRIAPVPVACYPNAGLPNAFGGYDEAPET
                     TSSLLEEFARAGLVNLVGGCCGTTPEHTAMIAAAVKDVTRREIPTFDDRVPTFSGLEP
                     FQIRTDTGFVMIGERQNVTGSAKFRRLIESGDFEAAVDVALDQVRSGANMLDVNMDAD
                     LLDAEEAMTRFLNLIATEPEIARVPIMVDSSSWDTLEAGLKCIQGKGVVNSISLKEGE
                     EDFLAKARKVRHYGAAVVVMCFDEEGQAGTVERKIEIAERAVHLLTTEAGFDPSDIII
                     DPNILAIATGLEEHDDYAKAFIEATREIKRRCPGVHVSGGVSNLSFSFRGNEPVRRAI
                     HSAFLYHAIAAGLDMAIVNAGQLEVYADIPKDMLEHVEDIIFNRRADATERMVIFAET
                     IKGGGLERQDDLTWREASVQDRLSHALVHGIVDYIEDDTEEARQAYDRPLEVIEGPLM
                     DGMKIVGDLFGSGKMFLPQVVKSARAMKKAVAYLEPFMEQEKADAGLSIADSAQGTLV
                     TATVKGDVHDIGKNIVGVVLGCNNYRVIDLGVMAPAELILDTAIKEKADIVGLSGLIT
                     PSLTEMVNVATEMQRRGMNLPLLIGGATTSRQHTAVKIAPAYEQPVVHVIDASRVVGV
                     VSNLLDPGRKETLDAENRVDQDRLRALHAEKEKTPLLPYRKALANRSPIEWRTDDVAP
                     PPFLGAQVVEPALTDLRPLIDWTFFFTAWELKGRYPQILDHPQQGEAARELFENGNEL
                     LDAIIADGSIQARGVYGFWPAQAEGDDIVLADGSVFPMLRQQVDHGADVDRPNRSLAD
                     YIAPPESGLADNLGGFAVTAGL"
BASE COUNT          438 a          856 c          816 g          352 t
ORIGIN      
        1 ttgctcgctg ggtgccagcg agatgcggcc ctacgtcgag gcgttctcgc gcatcgcccc
       61 ggtgcctgtc gcctgttacc ccaacgccgg gctcccgaac gcgttcggcg gctacgacga
      121 ggcccccgag accacgagct cgctgctcga ggagttcgcg cgtgccggcc tggtgaacct
      181 ggtgggcggc tgctgcggca ccacgcccga acacacggcg atgatcgcgg cggcggtcaa
      241 ggacgtgacc cggcgggaga tccccacctt cgacgaccgc gtgcccacgt tcagcggcct
      301 cgagccgttc cagatccgca cggacaccgg gttcgtgatg atcggcgagc gccagaacgt
      361 caccggcagc gcgaagttcc ggcgcttgat cgaatcgggt gacttcgagg ccgccgtcga
      421 cgtggcgctc gaccaggtgc gcagcggcgc caacatgctc gacgtcaaca tggacgccga
      481 cctcctcgat gccgaggagg ccatgacccg cttcctcaac ctgatcgcca ccgagcccga
      541 gatcgcccgg gtgccgatca tggtcgacag ctccagctgg gacaccctcg aggccgggct
      601 gaagtgcatc cagggcaagg gcgtcgtcaa ttcgatcagc ctgaaggagg gcgaggagga
      661 cttcctcgcc aaggccagga aggtccgtca ctacggcgcg gccgtggtcg tgatgtgctt
      721 cgacgaggag ggccaggcgg gcaccgtcga gcgcaagatc gagatcgccg agcgcgcggt
      781 tcacctgctc accaccgaag ccggcttcga tcccagcgac atcatcatcg accccaacat
      841 cctggcgatc gccaccgggc tcgaggagca cgacgactac gccaaggcat tcatcgaagc
      901 cacccgtgag atcaagcgcc gctgtccggg cgtccacgtg agcggcggcg tgtccaacct
      961 gagcttctcc ttccggggga acgagcccgt gcggcgcgcg atccactccg cgttcctgta
     1021 tcacgcgatc gccgcgggcc tggacatggc catcgtgaac gcggggcagc tcgaggtcta
     1081 cgcggacatc cccaaggaca tgctcgaaca cgtcgaggac atcatcttca accggcgcgc
     1141 ggacgccacc gaacgcatgg tgatcttcgc cgagacgatc aagggcggag ggctcgagcg
     1201 ccaggacgac ctcacctggc gggaggcctc ggtgcaggat cgcctctcgc acgcgctggt
     1261 gcacggcatc gtcgactaca tcgaggacga caccgaggag gcccgccagg cctacgaccg
     1321 cccgctcgag gtgatcgagg gcccgctgat ggatggcatg aagatcgtcg gcgacctgtt
     1381 cggcagcggc aagatgttcc tcccgcaggt ggtgaagtcg gcgcgcgcca tgaagaaggc
     1441 ggtcgcctac ctggagccgt tcatggagca ggagaaggcc gacgccggtc tctcgatcgc
     1501 cgacagcgcc cagggcaccc tggtcaccgc cacggtcaag ggcgacgtcc acgacatcgg
     1561 caagaacatc gtcggcgtcg tgctcggctg caacaactac cgcgtgatcg acctcggggt
     1621 gatggccccg gccgaactga tcctggacac cgccatcaag gagaaggccg acatcgtggg
     1681 gttgtccggg ctgatcacac cgtcgctcac cgagatggtg aacgtggcca ccgagatgca
     1741 gcgccgcggc atgaacctgc ccctgctgat cggcggcgcc accacatcgc gacagcacac
     1801 ggcggtgaag atcgcgccgg cgtacgagca gccggtcgtg cacgtcatcg acgcctcccg
     1861 cgtggtcggg gtggtctcca acctgctcga cccgggacgc aaggagaccc tggacgccga
     1921 gaaccgcgtc gatcaggacc ggctcagggc cctgcacgcc gagaaggaga agacgccgct
     1981 cctcccctac cgcaaggcgc tcgcgaaccg cagcccgatc gagtggcgaa ccgacgacgt
     2041 ggcccccccg ccgttcctcg gtgcccaggt ggtggaacct gcgctcaccg acctgcgacc
     2101 gctgatcgac tggacgttct tcttcacggc gtgggagctg aagggccgct acccccagat
     2161 cctggaccac ccccagcaag gggaggcggc gcgcgaactg ttcgagaacg gcaacgagct
     2221 gctcgacgcc atcatcgccg acggctccat ccaggccagg ggggtctacg gcttctggcc
     2281 cgcccaggcc gaaggtgacg acatcgtgct cgccgatggt tcggtcttcc cgatgctgcg
     2341 ccagcaggtg gatcacggcg ccgacgtcga ccggccgaac cggtcgctcg ccgactacat
     2401 cgctccgcct gagagcgggc tcgccgacaa cctgggtggc ttcgcggtca cggcgggact
     2461 cg
//
LOCUS       DATDBZ010000386         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_105060, whole genome shotgun sequence.
ACCESSION   DATDBZ010000386 DATDBZ010000000
VERSION     DATDBZ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105060"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VIX62_09210"
     CDS             complement(<1..621)
                     /locus_tag="VIX62_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5860448.1"
                     /translation="MSAERIETDVLVVGGGPGGAAAGYHLARHGIDVTVVDKASFPRE
                     KVCGDGLTPRSVAAMLRMGIDTADPGFERVKGLRVHSRTTTIELPWPELSSWPDYGLV
                     MPRSRFDSLLIERAQKAGARLMEQTEAVAPIMEGGWVVGADVRPAQDREAEPTQIRAR
                     FVVAADGAASRFAKPAGVQRDDSRPLGIAARRYYRTSYHPGPWFESW"
     gene            complement(775..1200)
                     /locus_tag="VIX62_09215"
     CDS             complement(775..1200)
                     /locus_tag="VIX62_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5860449.1"
                     /translation="MSDDLDLLRRAFRDVTSCRIGTVRPDGGPHVATRWFVWLEEELW
                     VSTRVGDTTWENASRDPRVSLVIDRGREWVELAGVHVEGVAESFPAEHPDLRAPMSTW
                     HEKYRTLLGGESFERLTQDVPVLGFLRVAPSRIDAWDHR"
     gene            complement(1197..2081)
                     /locus_tag="VIX62_09220"
     CDS             complement(1197..2081)
                     /locus_tag="VIX62_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5860450.1"
                     /translation="MSTEGAAVISDALRAPRARIDEALRAFLDEERSRVQAIEPRAIA
                     VLDETERLIRAGGKRLRPAFCFWGYRAAGGLDDDRIVRASAAIELLHTMALIHDDLMD
                     DTSQRRGVPSTAVRFAAEAIERGTPADAGRFGSAAAVVAGDLAAVLADRLLSRSGFDA
                     ESLARASEPYHAMRVDMALGQYLDVAGLAHEPDDARRVAWLKGGAYTVEGPLMIGAAF
                     ADGGAPQRAALHAFGRPLGEAFQLRDDLVDGEAASGTDAGLVEDLVRQARAALIGAPL
                     DAGAIAALDELASLVRPS"
     gene            2144..>2488
                     /locus_tag="VIX62_09225"
     CDS             2144..>2488
                     /locus_tag="VIX62_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY5860451.1"
                     /translation="MTRSQKLALATAGTTIVLFSVGGLVRGSGSGLGCSTWPECHPGQ
                     LFPSGTVHSIIEFSHRALAFLVIVLTLATGLVLWRQPTTSTSVKWAAALAFPLVLAQA
                     VLGGIVVATELNP"
BASE COUNT          369 a          954 c          804 g          361 t
ORIGIN      
        1 ccacgattcg aaccagggtc cggggtgata cgaggttcgg tagtagcgcc gcgccgcgat
       61 ccccagcggc cgcgagtcgt cgcgctggac cccggcgggc ttggcgaacc ggctcgccgc
      121 gccatcggcc gccaccacga aacgggcgcg gatctgcgtc ggctcggcct cgcggtcctg
      181 ggccggacgc acgtcggcac ccaccaccca gcctccctcc atgatgggcg ccacggcttc
      241 ggtctgttcc atcagccgcg caccggcctt ctgcgcccgc tcgatcagca gcgaatcgaa
      301 ccgagaacgc ggcatcacca gtccgtagtc cggccagctc gacagctcgg gccagggcag
      361 ttcgatcgtg gtggtgcggg agtgcacccg caggcccttc acacgttcga atccggggtc
      421 ggcggtatcg atgcccatcc gcagcatggc ggccacgctt cggggggtga ggccgtcccc
      481 gcagaccttc tcacggggga aggaggcctt gtcgaccacc gtgacgtcga tgccgtggcg
      541 ggccaggtgg tagccggctg cggcgccgcc ggggccaccc cccacgacca ggacgtcggt
      601 ctcgatccgc tcggcgctca cgcctgctcc ccctcggtcg ggccttcgat cgggttgccg
      661 gtgcttcgac cgcttgtgaa aagtttcaca aagtagagac taccgtacct agggcaaccg
      721 acacgaggct acgcccgccc ccggaacacc cgcaagaccc caggtgagga gccctcagcg
      781 gtggtcccag gcatcgatcc tcgagggcgc gacacggagg aatcccagga cgggcacgtc
      841 ctgcgtgagc cgttcgaacg actcgcctcc caagagggtc cggtacttct catgccaggt
      901 cgacatcggc gcgcgcagat ccgggtgctc ggcggggaag gactccgcca cgccctccac
      961 gtggacgccg gcgagctcca cccattcacg cccacgatcg atcaccaacg acacccgcgg
     1021 atcgcgggac gcgttctccc acgtggtgtc gccgacccgg gtcgagaccc acagctcctc
     1081 ttcgagccag acgaaccacc gcgtggccac gtgcggcccg ccgtcgggac gcaccgtgcc
     1141 gatccggcac gaggtcacgt cgcggaacgc ccggcggagc agatcgaggt cgtcgctcac
     1201 gacggcctca ccagcgacgc caactcgtcg agcgcggcga tcgccccggc atcgagcggt
     1261 gcgccgatca acgcggccct cgcctgccgg accaggtcct cgaccaggcc ggcgtccgtg
     1321 cccgacgccg cctcaccgtc gaccaggtcg tcgcgcaact ggaacgcctc gcccagcggc
     1381 cggccgaagg catgcaacgc ggcgcgctgt ggggcaccgc cgtcggcgaa ggcggcgccg
     1441 atcatcagcg ggccctccac cgtgtaggcg ccgcccttca gccacgcgac gcggcgtgcg
     1501 tcgtcgggtt cgtgcgccag gcccgccacg tcgaggtact ggcccagcgc catgtccacc
     1561 cgcatcgcgt gatacggctc ggacgcccgc gccaggcttt cggcatcgaa accggatcgc
     1621 gaaagcaatc ggtcggcgag caccgccgcg aggtcgcccg ccaccaccgc cgccgccgat
     1681 ccgaaccggc cggcatcggc cggggttccg cgctcgatgg cttcggcagc gaatcggacc
     1741 gccgtggagg gcacaccccg ccgctggctc gtgtcgtcca tcaggtcgtc gtggatcaac
     1801 gccatggtgt gcagcagctc gatggccgcc gaagcccgca cgatccggtc atcgtcgaga
     1861 ccgcctgccg cgcgatatcc ccagaagcag aaggccggcc gcaaccgttt gccaccggcc
     1921 ctgatcagcc gttcggtctc gtcgagcacg gcgatcgcgc gcggttcgat cgcctgcacc
     1981 ctcgatcgtt cctcgtccag gaacgcgcgc agcgcctcgt cgatccgcgc tcgcggagca
     2041 cgcaacgcat cggagatcac ggcggctccc tcggtggaca tgtgcccagt atctcgcacc
     2101 gacgccgccc gcctcgccca ctatgatggc gcgcgagccc ctcatgacgc gatcccagaa
     2161 gctcgctctc gccaccgccg gcacgacgat cgtgctcttc agcgtcggtg gcctggtccg
     2221 gggatcgggc tcggggctcg gctgttccac gtggcccgag tgccatcccg gccagctctt
     2281 cccctccggc accgtgcatt ccatcatcga gttctcgcat cgcgccctgg ccttcctcgt
     2341 gatcgtgctc accctcgcca ccggcctggt gctgtggcgg caacccacca cctccacgtc
     2401 tgtgaagtgg gcggccgcgc tcgcgttccc cttggtgctg gcccaggcgg tgctcggcgg
     2461 gatcgtggtc gcgaccgagc tcaacccc
//
LOCUS       DATDBZ010000387         2834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2788275, whole genome shotgun sequence.
ACCESSION   DATDBZ010000387 DATDBZ010000000
VERSION     DATDBZ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788275"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VIX62_09230"
     CDS             <1..592
                     /locus_tag="VIX62_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY5860452.1"
                     /translation="VSGLAVLVLLAPVVGRRSRRSLGNSDVAFWLISQRILAPVAIVI
                     PIYVLFQNLRLLDTLPALIITYTAANLPIVIWLMRDYFLNIPPDLEEAASVDGASPYR
                     IFRSVVIPVAKPGIVATGILVLVFAWNEYLIALFLTTANAQTMPVLVSAQNATRGPEW
                     WYMSVLILIMIIPVIVLAVILERFISRGALTGAVKG"
     gene            630..1877
                     /locus_tag="VIX62_09235"
     CDS             630..1877
                     /locus_tag="VIX62_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY5860453.1"
                     /translation="MTLVIGMNSGSSFDGIDAVLIEIGVDGEGQPVRPRFIDGLAYDW
                     PRKVEERILPLFENQSSVFELTRINYAAGAVYALAAQALIDRSGVDPAKVLAIGVDGQ
                     TIYQEAPDRPRMAAMSPDADLVDRWLDGPYAAGIFIGEPAIIATHTGIPVVSNFRPSD
                     HALGGTGAPMMQYLDWVSFRDIGPILTLNIGGIANCHLAAKDKSRMRAFDTGPGNVMI
                     DHAMRRFYDRGYDRNGEVAATGTIHQPLIDYLLEHPFYARKPPRSAWRLDFGSSYADT
                     MLDTFSDLSREDIIATLTRFTAISITKAITELMGDTSGVDTLICSGGGTRNPVLMGHL
                     AELAPVRVTTSDEYGIPAQYKEAIKFGTLAYANLNLLPNNIPACSGATAFTIMGKVTW
                     PPTMVRTDPAGDGAIVPAMSRQA"
     gene            1915..>2834
                     /locus_tag="VIX62_09240"
     CDS             1915..>2834
                     /locus_tag="VIX62_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5860454.1"
                     /translation="MTERVGWGIMGTGGIAHVFADAIARSDTGTLVAVSSREAAKAEA
                     FATETGASRAHASHEGLLADPAVKIVYVSTLHPYHVELAIAAAQAGKHVLCEKPIAIT
                     ASGAARVIEAARAANVFLMEAFAFRLHPQTTRLMELIEGGAIGDVRMMSLAWGFDFGS
                     TDGSGYHLDDTRGGGSILDNGCYPVAFARRVAGSSVGRVFRDPDQVVGTGLLHPDSRV
                     DWDAQALLWWEGGISAQISGTLRTEIDKTVLILGSGGLIRLPAPYLPLRPDRFGGVPR
                     IHVERQGREPEEIAIDVTQNAYVIEADRVAG"
BASE COUNT          442 a          999 c          957 g          436 t
ORIGIN      
        1 cgtgtccgga ctggcggtcc tggtgctgct ggcaccggtg gtgggccggc ggtcgcggcg
       61 cagcctgggt aactcggatg tcgcgttctg gctcatctcg cagcgcatcc tggcgcccgt
      121 cgcgatcgtc atcccgatct acgtcctgtt ccagaacctg cggctgctgg acaccctgcc
      181 cgcgctcatc atcacctaca ccgccgcgaa cctgcccatc gtcatctggc tcatgcggga
      241 ctacttcctg aacatcccgc cggacctcga ggaggcagcg tccgtggacg gcgcctcgcc
      301 ctaccggatc ttccgctcgg tggtcatccc ggtggccaag ccgggcatcg tggcgaccgg
      361 gatcctggtg ctcgtgttcg cgtggaacga gtacctcatc gccctgttcc tgaccaccgc
      421 gaacgcccag acgatgcccg tcctggtgtc agcgcagaac gctacccggg gccccgagtg
      481 gtggtacatg tcggtgctca tcctgatcat gatcatcccg gtgatcgtgc tcgccgtgat
      541 cctggaacgg ttcatctcgc gcggcgcgct caccggggcc gtcaaggggt gaggggggac
      601 ggatccctgg aactaggagt gacacaccca tgacgctggt gatcgggatg aacagcggct
      661 cctcgttcga tggcatcgac gcggtgctca tcgagatcgg cgtggacggg gagggccagc
      721 cggtccgccc gaggttcatc gacgggctcg cctacgactg gcccaggaag gtcgaggaac
      781 gcatcctgcc gctgttcgag aaccagagca gcgtgttcga gctgacgcgc atcaactacg
      841 ccgcgggtgc cgtgtacgcg ctcgcggcgc aggcgctcat cgacaggtcg ggggtcgacc
      901 ctgccaaggt gctggccatc ggcgtggacg gccagaccat ctaccaggag gcccccgacc
      961 gaccgcgcat ggcggccatg tcgccggatg ccgacctcgt cgaccgctgg ctggacggac
     1021 cctatgcggc gggtatcttc atcggcgagc cggccatcat cgcgacccac accggcatcc
     1081 cggtggtcag caacttccgg ccctccgacc atgccctggg cggcacgggc gcgccgatga
     1141 tgcagtacct cgactgggtc tcgttccggg acatcgggcc catcctcacg ctcaacatcg
     1201 ggggcatcgc caactgccac ctcgcggcga aggacaagtc gcggatgcgc gccttcgaca
     1261 cgggccccgg caacgtgatg atcgaccacg cgatgcgccg gttctacgac cgcggctacg
     1321 accgcaacgg cgaggtcgcg gcgacgggca ccatccacca gccgctcatc gactacctcc
     1381 tcgagcaccc gttctatgcc cgcaagccgc cccgcagcgc gtggcggctc gacttcggct
     1441 cgtcgtacgc ggacacgatg ctcgacacct tcagcgacct gtcccgcgag gacatcatcg
     1501 cgacgctcac gcgcttcacc gccatctcca tcaccaaggc catcaccgag ctgatgggtg
     1561 acacgagcgg cgtggacacg ctcatctgca gcggcggggg cacccgcaac ccggtgctca
     1621 tgggccatct cgcggagctc gcgccggtgc gcgtgaccac ctcggatgag tacggcatcc
     1681 cggcccagta caaggaggcc atcaagttcg ggacgctcgc gtacgcgaac ctcaacctgc
     1741 tgcccaacaa catcccggcc tgctcggggg cgaccgcgtt cacgatcatg ggcaaggtca
     1801 cctggccgcc cacgatggtg cgcaccgacc cggccggcga cggggccatc gtgcccgcca
     1861 tgtcgcgcca ggcatgacat cgcacgccac gaccacgatg aacggaggac ggtcatgacc
     1921 gagcgggtcg gctgggggat catgggcacg ggcggcatcg cccacgtgtt cgcggacgcc
     1981 atcgcgcgat cggacacggg gacgctggtc gcggtgtcca gccgcgaggc ggcgaaggcc
     2041 gaggcgttcg cgaccgagac cggcgcctcg cgtgcccacg cgtcgcacga ggggctgctt
     2101 gcggatcccg cggtcaagat cgtgtacgtc tcgacgctcc acccgtacca cgtggagctg
     2161 gccatcgccg cggcgcaggc gggcaagcac gtcctgtgcg agaagcccat cgcaatcacg
     2221 gcgagcggcg cggcacgcgt catcgaggcg gcgcgcgccg cgaacgtgtt cctgatggag
     2281 gcgttcgcgt tccggctgca cccccagacg acgcgcctca tggagctcat cgagggcggc
     2341 gccatcggcg atgtccggat gatgtcgctc gcgtggggct tcgacttcgg ctccacggac
     2401 ggctcgggct atcacctcga cgacacacgg ggcggcggct cgatcctcga caacggctgc
     2461 taccccgtgg cgttcgcgcg gcgcgtggcg ggctcatcgg tgggcagggt gttccgcgac
     2521 cccgaccagg tcgtgggcac cgggctgctc caccccgaca gccgggtgga ctgggatgcc
     2581 caggcgctcc tgtggtggga gggtggcatc agcgcccaga tctccgggac cctgcggacc
     2641 gagatcgaca agacggtgct catcctgggc agcggcgggc tcatccggct gccggcgccg
     2701 tatctgccgt tgcggcccga ccgcttcggt ggcgtgcccc gcatccacgt ggagcggcag
     2761 ggcagggagc ccgaggagat cgccatcgac gtcacccaga acgcctatgt catcgaggcg
     2821 gaccgggtgg cggg
//
LOCUS       DATDBZ010000388         2960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1567362, whole genome shotgun sequence.
ACCESSION   DATDBZ010000388 DATDBZ010000000
VERSION     DATDBZ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567362"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VIX62_09245"
     CDS             complement(<1..228)
                     /locus_tag="VIX62_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEY5860455.1"
                     /translation="MERVVVALGGNALLRQGAEDTFEEMYRASREAAERIADIAADGW
                     EVVVTHGNGPQVGRILLQQEAAREVVHPMPLD"
     gene            complement(216..1052)
                     /locus_tag="VIX62_09250"
     CDS             complement(216..1052)
                     /locus_tag="VIX62_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5860456.1"
                     /translation="MSHVEPFRVGDVEIAVLCQGWAPLPLADESPGHDVDWAEERRAS
                     PWAFAGDAHWQWHVQAFIVRTPSGVTMVDTGLGGNRPVVPWAPGEGLTAEQAYEDAGV
                     DPSEVSTVVLTHMHPDHSGGIRGSDGQPRFANARHVLHPADWAFLTGELSEFSALARP
                     GLHRVHELGMLDLGEGNREVAPGIEVRHTPGHTPGHRSVLVHGGDEVMLLTGDLLFLP
                     IQFASPSWGSSHDEDPDAGARSRTAIVSAAADGGWVVGVPHFAEPFGTVTDEGWSSRW
                     NG"
     gene            complement(1063..1680)
                     /locus_tag="VIX62_09255"
     CDS             complement(1063..1680)
                     /locus_tag="VIX62_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY5860457.1"
                     /translation="MTEPAGSLGATGVVLAGGRSTRFGSDKLAATYRGMPLLHHAVLR
                     LVEVTHDVVVVLPPDAEPPALPPGVPVRYVHDAVEGQGPLEGALSGLSSVVTEFAVLV
                     GGDMPELSTAVVIEMLRVAAASGADAVALQDGERARPLPLVVRAEPAREVAHALRHAG
                     EHRLRSLQQGLRTAVIDETTWHALDPNRGSLRDIDEPADIEHPST"
     gene            complement(1673..2665)
                     /locus_tag="VIX62_09260"
     CDS             complement(1673..2665)
                     /locus_tag="VIX62_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5860458.1"
                     /translation="MSQAVPLEPPRRLLSIEVDGDEVRVPEGATILDACRSQGVDTPT
                     LCFADNLTPVNACRVCVVEVEGARTLVPACSRAAEDGMKVKTDTERVERSRKLVLELL
                     ASSVEMDLTSPDVHRWMQEYETDPDRFGATMPELPAGERDAREPGHHHDPVDPMVAEG
                     VAQPIKIDNELYVRDYSRCILCYKCVEACGVDAQNTFAIAVAGRGFDARISTEYSVGL
                     DESACVYCGNCIGVCPTGALMFTSEHEMREAGTWDEDEQTVTSTICPYCGVGCQLDLH
                     VQDNEIVKVTSPADNSVTNGHLCIKGRFGFQYVQARKGDDPAARKRRQRREARD"
     gene            complement(2682..>2960)
                     /locus_tag="VIX62_09265"
     CDS             complement(2682..>2960)
                     /locus_tag="VIX62_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY5860459.1"
                     /translation="SCGQCVPCRVGTVRQEEALHRLAHDRTIGSHADEMALLADVAQV
                     MRDASICGLGQTAANAVQSAIEGLGVFGAVSATAADGDVPTPTEEQES"
BASE COUNT          418 a         1072 c         1025 g          445 t
ORIGIN      
        1 gtcgaggggc atagggtgca cgacctcccg cgccgcttcc tgctgcagaa ggatccggcc
       61 cacctgtggg ccgttcccgt gggtgacgac cacctcccag ccgtcggcgg cgatgtcggc
      121 gatgcgctcg gccgcctcgc ggctggcgcg gtacatctcc tcgaacgtgt cctcggcccc
      181 ttggcggagc agggcgtttc cgcccagcgc cacgactacc cgttccaccg ggagctccag
      241 ccctcgtcgg tcaccgtgcc gaacggctcc gcgaaatgcg gcacgcccac gacccagccg
      301 ccgtcggcgg cggccgacac gatcgcggtc cgtgatcgcg caccggcgtc gggatcctcg
      361 tcgtggctcg atccccagga cgggctcgcg aactggatcg gcaggaacag gagatcgccg
      421 gtgagcagca tgacctcgtc cccaccatgg accagcacgc tgcggtggcc cggggtgtgg
      481 ccgggcgtgt gtcggacctc gatcccgggg gccacctcgc ggttcccttc gcccaggtcc
      541 aacatcccca gttcgtgcac gcggtgcaga cccggccgtg ccagcgcgga gaactcggac
      601 agttccccgg tcaggaacgc ccagtccgcc gggtgcagga cgtggcgggc gttggcgaag
      661 cgcggctggc cgtcgctccc tcggatcccg cccgaatggt cggggtgcat gtgggtcagc
      721 acgaccgtgg agacctccga tggatcgacg cccgcatcct cgtacgcctg ctctgcggtc
      781 aggccctccc ccggcgccca cgggacgacc ggacggttcc ctcccaggcc cgtgtccacc
      841 atggtgacgc cgctcggcgt gcggacgatg aacgcctgga catgccactg ccagtgggcg
      901 tcaccggcga acgcccaggg ggaggcccgc cgctcctcgg cccagtccac gtcgtgcccc
      961 ggcgattcgt cggccagcgg cagtggtgcc catccctggc acaggacggc gatctcgacg
     1021 tcgcccacgc ggaacggctc gacgtggctc atggcggcga gtctatgtcg aggggtgctc
     1081 gatatcggca ggctcgtcga tgtcccggag ggacccgcgg ttcggatcca gagcgtgcca
     1141 ggtggtctcg tcgatcacgg cggtgcgcaa cccctgctgc aacgagcgga ggcggtgctc
     1201 gcccgcgtgc cggagcgcgt gcgcgacctc gcgagcgggc tcggcgcgaa ccacgagcgg
     1261 caacggacgg gctcgctcgc cgtcctgcag ggccacggcg tcggcccccg aggcggcggc
     1321 cacccgcagc atctcgatca ccaccgcggt ggagagctcg ggcatgtcgc cgcccaccag
     1381 caccgcgaac tcggtcacga cgctcgagag cccgctgagc gccccttcca gcggcccctg
     1441 cccctcgacc gcgtcgtgca cgtagcgaac gggcactccc ggcggcaacg cgggaggttc
     1501 ggcgtcggga ggcagcacca ccacgacgtc gtgcgtcacc tcgaccagtc gcaggaccgc
     1561 gtggtgcagc agcggcatgc cgcgataggt ggccgcgagc ttgtccgagc cgaaccgcgt
     1621 ggatcgccca ccggccagca ccacaccggt ggcgccgagc gaccctgccg gttcagtcac
     1681 gcgcctcgcg acgctggcga cgcttgcggg ccgcgggatc gtcgcccttg cgcgcctgca
     1741 cgtactggaa gccgaaccga cccttgatgc acaggtggcc gttcgtcacg gagttgtccg
     1801 ccgggctcgt caccttcacg atctcgttgt cctggacatg caggtcgagc tggcagccga
     1861 ccccgcagta cggacagatc gtgctggtga cggtctgctc gtcttcgtcc cacgtgccgg
     1921 cttcgcgcat ctcgtgttcg gaggtgaaca tcagcgcgcc ggtgggacag actccgatgc
     1981 agttcccgca gtagacgcac gcgctctcgt cgaggccgac cgaatactcc gtggagatcc
     2041 gcgcgtcgaa cccgcgcccg gccaccgcga tcgcgaacgt gttctgtgcg tcgacaccgc
     2101 aggcctctac gcacttgtag cagaggatgc agcgcgagta gtcgcgcacg tagagctcgt
     2161 tgtcgatctt gatgggctgg gcgacacctt cggccaccat cgggtcgact gggtcgtggt
     2221 gatgccccgg ttcgcgggcg tcgcgctcac ctgccgggag ttcgggcatc gtggccccga
     2281 accggtccgg atccgtctcg tactcctgca tccagcggtg gacgtcgggg ctggtcagat
     2341 ccatctcgac gctggaggcc agcagctcca gcaccagctt gcgcgaacgc tccacgcgct
     2401 cggtgtcggt cttgaccttc atcccgtcct ccgccgcacg gctgcaggcg ggaaccagcg
     2461 tgcgggcacc ctcgacctcg acgacacaca cgcggcaggc gttgacgggc gtgaggttgt
     2521 cggcgaagca cagggtgggc gtgtccacgc cctgggaacg gcaggcgtcg aggatcgtgg
     2581 cgccttcggg cacgcgcacc tcgtcgccgt cgacctcgat cgagagcaga cggcgcggcg
     2641 gctcgagcgg cacggcctga ctcatcgcgc cggtcccatc gtcatgactc ctgttcctcg
     2701 gtgggcgtgg ggacgtcgcc atcggcggcg gtggccgata cggcgccgaa cacgccgagc
     2761 ccttcgatgg cggactgcac ggcgttggcc gccgtctggc cgagcccgca gatcgaggca
     2821 tcgcgcatca cctgggcgac atcggcgagc agggccatct cgtcggcgtg cgacccgatc
     2881 gtgcggtcgt gcgcgagccg gtgcaaggcc tcttcctggc gcacggtgcc cacgcggcag
     2941 ggcacgcact ggccgcacga
//
LOCUS       DATDBZ010000389         3411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_950906, whole genome shotgun sequence.
ACCESSION   DATDBZ010000389 DATDBZ010000000
VERSION     DATDBZ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950906"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VIX62_09270"
     CDS             <1..410
                     /locus_tag="VIX62_09270"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEY5860460.1"
                     /translation="TYRIPYVWARTTVERRGSQVRYRSRRRWPGPRGASLALQVRLGD
                     TLDARDLNDLERFLICRWRLYSPGPATLPARGVPLLATQVEHPAWPLRRAVVESLHER
                     LVAATGLPSPTGPPLVHASPGVAVRFGARRQVP"
     gene            complement(424..702)
                     /locus_tag="VIX62_09275"
     CDS             complement(424..702)
                     /locus_tag="VIX62_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860461.1"
                     /translation="MIVALFGLLMPRFIMVLLWLFSDYLSEAYGTWVWPLIGFFILPT
                     TTLCYAVAVQELDGFTGWGAALTILGLLVDLGVLGRGRGVFKGSRDAV"
     gene            complement(699..1490)
                     /locus_tag="VIX62_09280"
     CDS             complement(699..1490)
                     /locus_tag="VIX62_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEY5860462.1"
                     /translation="MLRFLVKPRWIAFTVFAVVVAGVCFRLAFWQLDRLDGRRYYNDL
                     FRAGMTQAPEPIESLLAAPPEGPLVFRQVSATGRYDAAEETVLYGRPLDGRPGNHVLT
                     PLILDDGRAVIVDRGWVPFEPTYPPIAASAPPGGTVEITGLLGAGTHLEESPAVQRDD
                     GRITVLSRVDLEALQEQVPYELLPMYVTLRTQHPEQGDLPVPEPPPELTDGPHLGYAF
                     QWFAFGTIALVGWVILIRREALDRRGSTTQTEAGSPEDPEGMDGA"
     gene            complement(1539..2318)
                     /locus_tag="VIX62_09285"
     CDS             complement(1539..2318)
                     /locus_tag="VIX62_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5860463.1"
                     /translation="MHGESQDKVALVTGAGSPTGIGFACARVLAREGASVAVASTTDR
                     IHERAATLHGDGADAAGFIADLTDRSQARRMVAEVQQLFGRIDIVVNNAGMVNVGMDG
                     ENFLDFIDMNDADWDLDIAMNLHTAYNVTKPVVPGMIERGWGRIVMVSSVTGPFVTNP
                     GSTGYGAAKAGMDGLMRGLALELGPHGITANSVAPGWIASGSQVPDEADAGRFTPIGR
                     SGTPEEIAEVVAFLASDRSSYVTGQSLVVDGGNTIQEDKGP"
     gene            2493..2666
                     /locus_tag="VIX62_09290"
     CDS             2493..2666
                     /locus_tag="VIX62_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY5860464.1"
                     /translation="MTERRTRLLEDRGATAVEYALMLALIAMVIFGAVMFLGSQTDGQ
                     FEKVGDCFPPTTC"
     gene            2713..>3411
                     /locus_tag="VIX62_09295"
     CDS             2713..>3411
                     /locus_tag="VIX62_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY5860465.1"
                     /translation="MTSVRLLALQEVDTTIDRLSTRRAALEGGGALAEARELADAAEH
                     RLGEFGLKLDELGRDQMRFEHEIESMTQKEHVEQTRLYDGSVANAKELEALQHDIENL
                     KKRRADREDELLVVLEQREALEAGAAEARDTSEQLRATVDQVAGASAQELAAIVAELE
                     QRTAERASIAAEIDPELLELYEDLRSQKKGVGAATLVDGVCQGCHEQLSAMQLDKLKK
                     TDGVRRCEHCRRILV"
BASE COUNT          550 a         1155 c         1191 g          515 t
ORIGIN      
        1 agacctaccg catcccgtac gtgtgggccc gcaccacggt cgagcggcgg ggatcgcagg
       61 ttcgctatcg gagccgtcgt cgctggccgg gaccccgtgg cgcatcgctg gcgctgcagg
      121 tgcggctcgg cgatacgctg gacgctcgtg acctgaacga cctggaacgg ttcctcatct
      181 gccggtggcg cctctacagc cccggcccgg cgaccttgcc ggcgcgaggg gtgccgttgc
      241 tggccaccca ggtcgagcac ccggcctggc cgctccgtcg cgccgtggtc gagtcgttgc
      301 acgagcgtct ggtcgccgcg acagggctgc cgtcgccgac gggaccgcct ctcgtgcatg
      361 cctcaccagg ggtcgcggtg cggttcggcg cacgccgcca ggtcccatga tgctcgcgca
      421 ccttcacacg gcgtcgcgtg agcccttgaa gacccctcgg ccgcgaccca acacgcccag
      481 gtcgaccaac aggccgagga tcgtgagcgc cgcgccccat ccggtgaacc cgtcgagctc
      541 ctgcaccgcg accgcgtaac acagggtggt ggtcggcagg atgaagaagc cgatcaacgg
      601 ccacacccac gtgccgtagg cctcgctgag gtagtcggag aacagccaca gcagcaccat
      661 gatgaaccgc ggcatcagca gtccgaacag cgcgacgatc atgctccgtc catcccctcg
      721 gggtcttcgg gtgatcccgc ctcagtctgc gtggtggatc cgcgtcggtc cagggcctcc
      781 cgtcggatca ggatcaccca gccgaccagc gcgatcgtgc cgaaagcgaa ccattggaac
      841 gcgtacccga ggtgggggcc gtcggtgagt tcgggcggcg gttccgggac cggcaggtcg
      901 ccctgctcgg gatgctgggt gcgcagggtc acgtacatcg gcagcaactc gtagggcacc
      961 tgctcctgca gagcctcgag gtcgacacga gagagcaccg tgatgcgccc gtcgtcgcgt
     1021 tgaacggcgg ggctctcctc gagatgcgtg ccggcgccca acagcccggt gatctcgacc
     1081 gtcccgccgg gaggggccga cgccgcgatc ggcggatacg tgggctcgaa cggcacccag
     1141 cctcggtcga cgatgaccgc ccttccgtcg tcgaggatga gcggggtgag cacatggttg
     1201 ccgggcctgc cgtcgagcgg ccggccgtac aggaccgtct cctcggcggc gtcgtagcgt
     1261 ccggtggcgc tcacctgccg gaacacgagg gggccttcgg ggggagccgc caggagcgac
     1321 tcgatcggtt cgggcgcctg ggtcatcccc gcacggaaga ggtcgttgta gtagcgacga
     1381 ccgtccagcc ggtcgagttg ccagaacgcg agccggaaac acacacccgc gaccaccacc
     1441 gcgaacaccg tgaaggcgat ccaccgtggc ttcaccagga atcgcagcat cgcaacgagc
     1501 atagtggcga ccctcgcgct ggccgccggg ccggagggct aggggccttt gtcctcctgg
     1561 atcgtgttgc cgccgtcgac cacgagcgat tggccggtga cgtagctcga tcgatccgag
     1621 gcgaggaacg ccacgacctc ggcgatctcc tcaggcgttc ccgagcgccc gatcggggtg
     1681 aagcgtccgg cgtctgcttc gtcggggacc tgggagccgc tggcgatcca cccgggtgcg
     1741 accgagttcg ccgtgatccc gtgcggaccg agttcgaggg cgaggccccg catcagaccg
     1801 tccatgccgg ccttggcggc gccgtagccc gtggagccgg ggttcgtcac gaacggaccc
     1861 gtcaccgacg acaccatcac gatcctgccc cagccgcgct cgatcatgcc cggcacgacg
     1921 ggcttggtca cgttgtaggc ggtgtgcagg ttcatcgcga tgtccagatc ccagtcggca
     1981 tcgttcatgt cgatgaagtc gaggaagttc tcgccgtcca tgcccacgtt caccatgccg
     2041 gcgttgttga ccacgatgtc gatacgtccg aagagctgct gcacctccgc gaccatgcgc
     2101 cgggcctgtg atcggtctgt gaggtcggcg atgaaccccg cagcgtcggc gccgtcgccg
     2161 tggagcgtgg cggcgcgttc gtggatccgg tcggtggtgg aggcgacggc gaccgaggcg
     2221 ccctcccggg cgagcacccg cgcgcacgcg aacccgatcc ccgtggggct gcccgccccg
     2281 gtgaccagcg ccaccttgtc ctgggattct ccgtgcatga ggcgagaagc ctaccgggag
     2341 cgaccatcgg gcggctcaac tttccgcccg gttcgtgccg atacaccacc caagacggcg
     2401 cgaggggccg cccgcatcgg tgcgggcggt ccgcgcgacg gtgattgggg gaccagatgt
     2461 tcctgagaca gttcgtacga gctcaggtct ggatgacgga gcgtcgcacc cggctgctgg
     2521 aggatcgggg cgcgacggcc gtcgagtatg cgttgatgct ggcgttgatc gccatggtga
     2581 tcttcggcgc ggtgatgttc ctcggctccc agaccgatgg gcagttcgag aaggtcggag
     2641 actgcttccc gccgaccacc tgctaggacc gcaccacgcg gacgaagcgg catcggctag
     2701 gcttcgcctg ccatgacgtc cgttcgcctg ctcgcactac aagaggtcga tacgaccatc
     2761 gatcgattga gcacgcgtcg cgcggcgctg gaaggcggtg gggcgctcgc cgaggcgcgt
     2821 gagctcgccg atgccgccga gcaccggctg ggggagttcg ggctgaagct cgacgagctc
     2881 ggccgtgacc agatgcggtt cgagcacgag atcgaatcga tgacccagaa ggagcacgtc
     2941 gagcagacgc gcctgtacga cggttcggtc gccaacgcca aggaactcga agcgctccag
     3001 cacgacatcg agaacctgaa gaagcgccgg gccgatcgcg aagacgaact cctggtcgtg
     3061 ctcgagcagc gcgaggccct cgaggccggc gcggccgagg cgcgggacac gtccgagcag
     3121 ctccgtgcca cggtcgatca agtggcggga gcctcggcgc aggagctcgc cgccatcgtc
     3181 gccgagctcg agcaacggac ggccgaacgc gcgtcgatcg ccgccgagat cgaccccgag
     3241 ctcctcgagc tctacgagga ccttcgctcc cagaagaagg gcgtgggcgc cgcgacgttg
     3301 gtcgacggtg tctgccaagg ctgccacgag cagctctccg cgatgcagct cgacaagctc
     3361 aagaagaccg atggggtccg gaggtgtgag cactgccggc ggatcctggt c
//
LOCUS       DATDBZ010000390         2060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1135600, whole genome shotgun sequence.
ACCESSION   DATDBZ010000390 DATDBZ010000000
VERSION     DATDBZ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135600"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..>2060
                     /locus_tag="VIX62_09300"
     CDS             35..>2060
                     /locus_tag="VIX62_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5860466.1"
                     /translation="MLRDARDKNKWRKIVRFRLWMPVVLQLLLIVAFAGYTTSRFDGF
                     INASNVTNILILAAPLAMVAMGQTHALLVGYLDLSVGAMVTIGVVVASFLIGPDTSSS
                     QILLGAGAVFLCALAVGAVNAVLVVGLKIPSIIATLATLSILNGISLTLRETPGGIIN
                     RDFTEWLRTGLGPVPIIFVVCIVVAAGLDYWLHATGQGLQLRATGFDDISAKRNGVRT
                     KWVRVRALLLSAIFATVAAFFVMTRSGIGNAAIGDSYALNSITAAVLGGAALSGGRAT
                     FLGALVASVLLALIISALPFLGLSAEHGLIIIGVLVLLGIVLFQLGDLKELVKRNYKR
                     ARRLAIGSRPPKQAEIPDLYPSGVDFRVVPTETKVIAGGAVLSLDPRLGEFANADVLI
                     EGDRIVEVRPGIGPIADAEVIDATGMIVMPGFVDTHRHIWEGLLRNIGTDVPLEGRSS
                     YISYVLHKLAPAYRPEDSYVGDLLSAMGAIDAGVTTLLDWSHIQGSPAHTDAVIHALK
                     DSGMRAVFAYGFPWWGKWEERQPSWFVRAANEHFSTKDQLLSLALAAPGPEFTDFEIT
                     RDHWKLARETGARITTHVGVGSYGRDGKVQEFGEAGLLGPDTTYIHCTTLNDTEIQMI
                     VDSGGTVSLASPVEMMMGHGMPPIQKFLDRGLSPSLSVDVETNVPSDMFNQ"
BASE COUNT          323 a          706 c          677 g          354 t
ORIGIN      
        1 gaccgtcgac accgcgagga tcgccttgcg caaggtgctt cgcgacgccc gtgataagaa
       61 caagtggcgc aagatcgtcc gcttccggct ctggatgccg gtggtgctcc agctcctgct
      121 gatcgtcgcc ttcgccgggt acacgacatc tcggttcgac ggcttcatca acgcctcgaa
      181 cgtcacgaac atcctgatcc tggccgcccc gctcgcgatg gtcgcgatgg gtcagacgca
      241 cgcactgctc gtcggatacc tcgacctgtc cgtgggggcg atggtcacga tcggcgtcgt
      301 agtcgcctcg ttcctgatcg gcccggacac gagttcgtcc cagatcttgc tcggggccgg
      361 ggcggtgttc ctctgcgcgc tggccgtggg agccgtgaac gccgtcctcg tggtcggtct
      421 gaagatcccg tcgatcatcg ccacgctcgc cacgctcagc atcctcaacg gcatatccct
      481 cacgcttcgg gagacgcccg ggggcatcat caaccgcgac ttcacggagt ggctgcgcac
      541 cggcctcggc ccggtgccga tcatcttcgt cgtctgcatc gtcgtggccg ccgggctcga
      601 ctactggctc cacgcgacgg gtcagggctt gcagctgcgg gcgacgggtt tcgacgatat
      661 ctccgccaag cgcaacggcg tccgcacgaa atgggtccgc gtgcgtgcgc tcctgctctc
      721 agcgatcttc gcgaccgtcg cggcgttctt cgtcatgacc cggtccggca tcgggaacgc
      781 ggcgatcggc gacagctacg cgctgaacag catcaccgcc gcggtattgg gcggcgcggc
      841 gctctcgggc gggcgcgcga cgttcctcgg cgccctcgtt gcgtccgtgc tcctcgcgct
      901 catcatcagt gcgctgccgt tcctcgggct cagtgcggaa cacggcctga tcatcatcgg
      961 cgtcctcgtg ctgctgggca tcgtgctctt ccagctcggc gacctgaagg agctcgtgaa
     1021 gcggaactac aagcgcgccc gccgtctcgc gatcgggagc cggcccccga agcaggccga
     1081 gatccccgac ctgtacccgt caggggtgga cttccgggtc gtgccgaccg agacgaaggt
     1141 gatcgcgggc ggcgccgtgc tgagcctcga tccccggctg ggggagttcg cgaacgccga
     1201 cgtgctgatc gagggagacc gcatcgtcga ggttcgcccg ggcatcggtc cgatcgccga
     1261 cgccgaggtg atcgacgcga ccggcatgat cgtgatgccc gggttcgtcg acacgcaccg
     1321 ccacatctgg gagggcctac tgcgcaacat cgggaccgac gtcccgctcg agggacgatc
     1381 cagctacata tcctacgtgt tgcacaagct cgcgcccgcg taccgtcccg aggactccta
     1441 cgtcggcgac ctgctctcgg cgatgggcgc gatcgatgcc ggcgtcacta ccctcctgga
     1501 ctggtcgcac atccaggggt cgccggcgca cacggacgcg gtgatccatg cgctcaagga
     1561 ctcgggcatg cgagcggtgt tcgcctacgg gttcccctgg tgggggaagt gggaggagcg
     1621 gcagccgagc tggttcgtgc gggcggcgaa cgagcatttc tccacgaagg atcagctgct
     1681 ctcgctcgcc ctcgcggccc ccggcccgga attcaccgac ttcgagatca cgcgagacca
     1741 ctggaagctc gccagggaga ccggggcccg catcacgacg cacgtcggcg tgggctccta
     1801 cggtcgcgac gggaaggtcc aggagttcgg ggaagcgggc ctgctcggac ccgacaccac
     1861 gtacatccac tgcacgaccc tgaacgacac ggagatccag atgatcgtcg acagcggtgg
     1921 cacggtctca ctcgcctcgc cggtggagat gatgatggga cacggcatgc cgccgatcca
     1981 gaagttcctc gaccgcggcc tcagtccgag cttgagcgtc gacgtcgaga cgaacgtgcc
     2041 cagcgacatg ttcaaccaga
//
LOCUS       DATDBZ010000391        10798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2184401, whole genome shotgun sequence.
ACCESSION   DATDBZ010000391 DATDBZ010000000
VERSION     DATDBZ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10798
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184401"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VIX62_09305"
     CDS             complement(<1..501)
                     /locus_tag="VIX62_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HEY5860467.1"
                     /translation="MNQKEPKTTHIVISDREPGLPFSKGLMASQVMVTGLSPYRAYQV
                     AEAIEDHLRERGFVSITSDELSDVALGVITEIAGDRYAKNFVRWREVERLDVPLVILI
                     GGATGVGKSTIATQLAARLGIVRVVATDAIREVMRSMLSSELMPTLHTSSFHADQALR
                     EPPGTTS"
     gene            complement(530..1858)
                     /locus_tag="VIX62_09310"
     CDS             complement(530..1858)
                     /locus_tag="VIX62_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HEY5860468.1"
                     /translation="MRTVVLVDGEHYPPVTRWAIATAIERGHDVVGAVFVGGIEKIEP
                     GSLPELGVPTLAAGDDRAGGLRDAIETWHPELVLDLSDEPVLGYRERMALAAVALTMG
                     VPYAGADFRLDPPAEAPPPPVPTLAVIGTGKRTGKTAISGEVARLAARRGLDPVVVAM
                     GRGGPPEPQVAEAGSIHLERLVELVRAGEHAASDYLEDALTTGVTTIGARRAGGGLAG
                     EPFVTNVPEAVGLAAERDPGLLILEGSGSAVPPIRWDAGVLVVPASAPPEYLGGYLGP
                     YRLLRSDLAVVTMAVGPTGSENLPALRSHILRYLDAARLLITDFIPVPLADVQGARAF
                     FATTAPGGVAARQVEHLEASHGCTVVGWSARLADRAGLAEDLDAAQGYDVLLTELKAA
                     AVDIGVERALAAGAEVVFVDNRAIVVEGSNDLDTALGDIVDLARTRRSER"
     gene            complement(1912..2700)
                     /locus_tag="VIX62_09315"
     CDS             complement(1912..2700)
                     /locus_tag="VIX62_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY5860469.1"
                     /translation="MVTLAHISDPHVGSPFFVPNLMNRVIAEINEMQPDAVVCSGDLT
                     AEGYRQEYKNWVAYAERIQSPILTVPGNHDSRNVGYLHFEDLIGPRMWSHDLQGVRIV
                     GVDSSEPDLNEGKVGRVHYDWIRHQFEEPAALKVFVLHHHLLPIPGTGRERSTVMDAG
                     DLLEVLINAGVHVVLSGHKHVPYVWRLENMYIANAGTVSSLRVRGYTKPCYNVLEFDG
                     SEVKIHRRFPFGESHVMTHFSLAGAQLHREHEAPIQQRTVPVGE"
     gene            2838..4013
                     /locus_tag="VIX62_09320"
     CDS             2838..4013
                     /locus_tag="VIX62_09320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEY5860470.1"
                     /translation="MSWLDVMLVALLVLSLLTGYRRGATLQVIGLIGLAVGVVAGVLL
                     APRVATLASSPSGAIALAMGTVLIGGAVGNLAGWVIGSRIRPRNEGSSIRRVDAVGGS
                     AISAIALLLATWFIALNLANGPFPGLARGIRDSRIVRTLDSTLPPPPSIVGELQRVLA
                     LLGFPDVFVGLPPQPAPPVEPPAGADARAATRAAADSTVEVLGRGCYQGFLNQGSGFV
                     VADGYVITNAHVVAGTNEQWVHSNEGDTAAAVVVFDPDLDIAVLHAPGLRADPLPLAR
                     GEVDRGAGGAILGYPGGGPLDAESAAVRQVIEPVGRNIYGEGEVRRRVYEVQSVIRRG
                     NSGGPFVLPDGRVAGVVFANSVVAEDVGYAIVSTQVLPSVKEAKASVEPVGTGSCTN"
     gene            complement(4021..4875)
                     /gene="thrB"
                     /locus_tag="VIX62_09325"
     CDS             complement(4021..4875)
                     /gene="thrB"
                     /locus_tag="VIX62_09325"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY5860471.1"
                     /translation="MPATSANLGPGFDSFGLALDLCNEVMIDTDAEPGVTWEGEGARE
                     LPVDGTDMVSTTIASVCAANGSPVPAIAVHGINRIPLERGLGSSSAAAVAGVVAADHL
                     LAAGASPSLRPERILALAADIEGHPDNAAPATLGGFTIAASDGFVRRLDPHPSLRPVV
                     LVPATRLPTKEARAALPPKVSREDAVFNAAHAALAVEALTQDPSLLGVALQDRLHQDA
                     RLALVPAVRDVFNELRAGDIPVCVSGAGPSLLAFETDARPVPAPGDGWTALRPGVRAS
                     GYEVSIEA"
     gene            complement(4897..5955)
                     /gene="thrC"
                     /locus_tag="VIX62_09330"
     CDS             complement(4897..5955)
                     /gene="thrC"
                     /locus_tag="VIX62_09330"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY5860472.1"
                     /translation="MPGAHQWRGLIEEYRDRLPVSEATPVVTLREGGTPLVRSEPLSD
                     ETGCEVWLKYDGANPTGSFKDRGMTLAISKALEEGAKAIVCASTGNTSASAAAYAAKA
                     GLTCAVLVPKGKVAVGKMAQTLVHGARVLEVEGNFDDSLELAKDLAERYPVTLVNSVN
                     PFRLQGQKTAAFEIVDALGRAPDMHLVPVGNAGNISSHWLGYSEYLADGLIAEPPQLM
                     GFQAQGAAPIVRGEVVKDPQTIATAIRIGNPASWDKAVAAATESEGAIGAVTDREILA
                     AYRRLAREGLFAEPASAASVAGLLHLHADGQLPSGATVVCILTGHGLKDPEWAIAGAA
                     NPVVVKPDPAAVAAELGL"
     gene            complement(5970..7262)
                     /locus_tag="VIX62_09335"
     CDS             complement(5970..7262)
                     /locus_tag="VIX62_09335"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY5860473.1"
                     /translation="MSERTIRVGMLGCGTVGAAVIRMLHEHADDIALRAGCRVEVTRV
                     AVRDPELDRDVPLTRQAFTTDGASVVTDPDIDVVCELIGGLEPARGLLLEAFAAGKPV
                     VTGNKELLANAGAELFEAADAADRDLFFEASVAGGVPLIRSLKESLAGERVTRMLGIV
                     NGTTNYILTQMSEHGWSFDQALAEAQSLGYAEADPTADVEGFDAAAKCAILASIAFNS
                     RVVAGDVYREGITAITAQDIADAARMGYVVKLLAIAELEDGEISARVHPAMIPAAHPL
                     ASVRDAFNAVFIEGPKVGQLMLYGRGAGGDPTAAAVVGDLVSAARNLVTGGRAIGCTC
                     ALERHIRPMDDTRGQYYLNLHVADRPGVLAEIAGDFAHNGVSIERVWQEGFADEATLV
                     FITHRAQEGALQKTLQELRESTAVRSVASVLRVEGEEP"
     gene            complement(7259..9139)
                     /gene="lysA"
                     /locus_tag="VIX62_09340"
     CDS             complement(7259..9139)
                     /gene="lysA"
                     /locus_tag="VIX62_09340"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY5860474.1"
                     /translation="MAEPSPRPSAADELPAPPWPATALRGPWGLEVGGLALTELAARF
                     GTPLLVVDEQEVRDRACAIAGRFPRVLYAVKAFTAHAVLRIVLDEGLDLLASTGGEVE
                     ACLRAGAPASRIVLHGNNKTDEELALAVGSGVSLVVVDHQAELERLDAIARSQGRVQR
                     VLLRVVPEVEVATHEAIATGHDASKFGMSLADAPGVIEAAVSMTGIRFDGIHAHVGSQ
                     VLDLEPYLRSLDRLVQTLADIRDRVGATAAVLDLGGGFGVTYVDERPLATDALAAAVT
                     ARLSERCAALDLPVPSLVVEPGRSIVANAGLTAYRVGSRKQVGNGRTLLAVDGGMSDN
                     VRPMLYDARYTVAAVGPSAGEDLATFTVVGRHCESGDTLAEDVHLPADAGPGDLLAFA
                     ATGAYTYSLASSYNRVGRPAVVGVRDGLARLWLRREDAADLDRLEGPAPRAAVVAEAP
                     ESITIRPAAPRDADSYIAFWTAVVAEGRYVRTERVSHSKRVYRSRFRRPWTDHEAQIV
                     AVDGQGTVVGHIYIQRERHPVTHHVATLGIAVAADHRGRGVGSALLADAIVWARGVGV
                     EKLVLSVYPHNTGAIALYRKFGFVQEGRLSRQSRKSYGDEDEILMAAWIAGEDLEGTD
                     RP"
     gene            complement(9132..>10798)
                     /gene="argS"
                     /locus_tag="VIX62_09345"
     CDS             complement(9132..>10798)
                     /gene="argS"
                     /locus_tag="VIX62_09345"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY5860475.1"
                     /translation="LVDWLQDGLREAAPALGIEGDLPTPGLLAPKQKGHGDFATNVAL
                     ALAKRAGRPPRDVAQAIADALPASPLVEKVEVAGPGFLNIFTADTWLHDALRSIADLG
                     EAYGRAEPTGRRVQVEFVSANPTGPLHVGHARNAALGDALARLLEAAGETVEREYYFN
                     DAGRQMDLFGASVVARYLQVLGREAELPEDGYHGEYVVEVARDIVAEHGDALADLPEP
                     EREARLLAEGTRRILAGVEQTLERFGVRFDVYFSEAELERKGEIAAAVERLRAADYAY
                     DADGATWFRSTAFGDDKDRVIVRSNGTHTYFGADCAYIVDKFARGFDHVVYVWGADHH
                     GDVARMKGAASAMGYDPDALEIVLYQFVSFQRGGEPVKMSKRAGTFISLDELIDEVGA
                     DAARFTLLSFSGDSAMNFDIETVKRQSMDNPVYYVQYGHARIASILRKAESVGVARTS
                     IADADLSLLRHEAELDLLRALADVPGQVAAAAELRAPQRITHLAQDLAGRFHRFYTEC
                     TVVNADEPALTHARLWLAAGTKQVIANLLGILGVSAPESMERTGDG"
BASE COUNT         1573 a         3785 c         3830 g         1610 t
ORIGIN      
        1 ggatgtggtg cccggcggtt cgcgcaacgc ctggtcggca tggaacgacg aggtgtgcag
       61 ggtcggcatc agctcggagg acagcatcga gcgcatcacc tctcggatcg cgtcggtggc
      121 caccacgcgc acgatgccca ggcgggccgc gagctgggtg gcgatcgtgg acttgcccac
      181 cccggtggcg cctccgatca ggatcacgag cgggacgtcc agccgctcca cctcgcgcca
      241 ccgcacgaag ttcttcgcgt agcggtcgcc ggcgatctcg gtgatgacgc cgagcgcgac
      301 gtcgctcagc tcgtcgctgg tgatcgagac gaacccgcgc tcgcgcagat gatcctcgat
      361 cgcttcggcg acctgatagg cccggtaggg cgacaggccg gtgaccatga cctgtgaggc
      421 catcaggccc ttggagaagg gcaagccggg ctcacggtcg gagatgacga tgtgggtggt
      481 cttgggttct ttctgattca taggctgact catccgtgtt tgaggctgtt caccgttcgc
      541 ttcggcgcgt tcgcgccagg tccacgatgt cccccagcgc cgtgtcgaga tcgttggatc
      601 cctccacgac gatcgcccgg ttgtccacga acacgacctc cgcccccgca gcgagagccc
      661 gctcgacgcc gatgtcgacg gcggcggcct tcagctccgt gaggaggacg tcgtatccct
      721 gggctgcgtc gagatcctcc gcgagcccgg cacgatcggc cagacgcgca ctccacccca
      781 cgaccgtgca tccgtggctc gcctcgaggt gttcgacctg tcgcgcggcc acgccgccag
      841 gcgccgtggt ggcgaagaac gcccgcgcgc cctgcacgtc tgccagcggc accgggatga
      901 aatcggtgat cagcagccgc gcagcatcca ggtaacgcag gatgtgggag cgcagtgcgg
      961 ggaggttctc ggacccagtt gggccgacag ccatggtaac aacggcgagg tccgagcgca
     1021 acagccggta cgggccgagg tagccaccca ggtattcggg cggtgcgctg gccggcacga
     1081 ccaggacccc ggcatcccat cggatcggcg gcacggcgga tccgctgccc tccaggatca
     1141 ggagcccggg atcgcgctcg gcggccagac ccaccgcttc gggcacgttg gtcacgaacg
     1201 gctctccggc caggccgccc ccggcccgac gcgcgccgat cgtggtgacc ccggtggtga
     1261 gggcgtcctc gaggtagtcg gaggcggcgt gctccccggc ccgcaccagc tcgacgagcc
     1321 gttcgaggtg gatcgatcct gcctcggcca cctgcggttc gggagggcct cccctcccca
     1381 tggccaccac cacgggatcg agcccccggc gggcggcgag ccgtgccacc tcgccactga
     1441 tcgcggtctt gccggtgcgc ttgccggttc cgatgaccgc gagggtgggg accggcggcg
     1501 gcggtgcctc cgccggcggg tcgagtcgga aatcggcccc ggcgtagggc acgcccatcg
     1561 tcagcgccac cgccgccaac gccatgcgct cgcggtagcc cagcaccggc tcgtcggaca
     1621 ggtcgagcac cagctcgggg tgccaggtct cgatcgcatc acggagcccg cccgcgcgat
     1681 cgtcgccggc cgcaagcgtc gggacgccga gctcgggcag ggaccccggc tcgatcttct
     1741 cgatgccacc gacgaacacg gcgccgacga cgtcgtgtcc gcgctcgatc gcggtcgcga
     1801 tggcccagcg ggtgaccggc ggatagtgct cgccgtcgac gagcaccacg gttcgcatcg
     1861 tcgaagactc ctttcgtcgg gagcggcgcg ggaacgtggg ttgcggttcg gctactcgcc
     1921 gaccgggacg gttcgttgct ggatcggggc ttcgtgctcg cgatgcagct gagcgcccgc
     1981 gagcgagaag tgcgtcatga cgtggctctc gccgaacggg aaccggcggt ggatcttgac
     2041 ctcggacccg tcgaactcga gcacgttgta gcacggcttg gtgtacccgc gaacccgcag
     2101 ggacgagacg gttcctgcgt tcgcgatgta catgttctcc agccgccaga cgtagggcac
     2161 gtgcttgtgt ccggacaaca cgacgtggac ccccgcgttg atcaggacct cgagcagatc
     2221 gccggcatcc atgaccgtgc tgcgctcacg acccgtgccg gggatcggga gcagatggtg
     2281 gtggagcacg aacaccttga gcgccgcggg ctcctcgaac tggtggcgga tccagtcgta
     2341 gtgcacccgg cccaccttgc cctcgttgag gtcgggttcg ctggagtcca cccccacgat
     2401 gcgcacgccc tgcaggtcgt gggaccacat gcgggggccg atcaggtcct cgaagtggag
     2461 gtagccgacg ttgcgtgaat cgtggttgcc cggcacggtg aggatcggcg actggatgcg
     2521 ctcggcatag gcgacccagt tcttgtactc ctggcggtaa ccctcagccg tgagatcgcc
     2581 actgcacacg acggcgtcgg gctgcatctc gttgatctcg gcgatcaccc ggttcatcag
     2641 gttgggcacg aagaacggcg aacccacgtg agggtcggag atgtgcgcca gggtgaccac
     2701 ggtggctcca gtctagttgc gcgtgcggac ggttggcgtc gggacgaacc ggttcatcct
     2761 ttggttcttc ggccggcgag cgccggtgtt gtagccgggc ttcaggtctg gatggagcgt
     2821 gggacgatac ctgcctggtg agttggctcg acgtgatgct cgtggcgctc ctcgtgctct
     2881 ccctgctgac ggggtaccgg cggggcgcca ccctgcaggt gatcggcttg atcggcctgg
     2941 ccgtcggcgt cgtggccggg gtcctgctgg cgccacgggt cgccacgctc gcctcgagcc
     3001 cgagcggggc gatcgccctg gccatgggca cggtgttgat cggtggcgcg gtgggcaacc
     3061 tcgccggctg ggtgatcggt tcgaggatcc ggcctcgcaa cgagggatcg tcgatcaggc
     3121 gcgtcgatgc ggtcgggggc tcggcgatct cggcgatcgc cctgctgctc gccacctggt
     3181 tcatcgcgct gaacctggcc aacggcccgt tccccgggct cgcgcgcggc atccgggact
     3241 ctcggatcgt caggaccctg gactcgacgc tgccgccgcc gccgtcgatc gtgggcgaac
     3301 tgcagcgggt gttggcattg ttgggcttcc ccgacgtgtt cgtcgggctg ccaccccaac
     3361 ccgcgccccc ggtcgagcca ccggccggcg ccgacgcacg ggcagccacc cgcgccgcgg
     3421 cggacagcac cgtggaggtg cttggacgcg gctgctacca gggcttcttg aaccagggca
     3481 gcgggttcgt ggtggccgac gggtacgtca tcaccaacgc ccatgtcgtc gccggcacga
     3541 acgaacagtg ggtccacagc aacgaaggcg acaccgcggc ggcggtcgtg gtgttcgatc
     3601 ccgatctgga catcgccgtg ctccacgctc cggggctgcg cgccgatcca ctccccttgg
     3661 cgcgcgggga ggtcgaccgg ggcgctggtg gtgccatcct cggctacccc ggtggaggcc
     3721 cgctcgacgc cgagtcggcg gccgtgcgcc aagtgatcga gccggtcggc cgcaacatct
     3781 acggcgaggg cgaggtgcgc cgtcgagtgt acgaggtcca atcggtgatc cggcgcggga
     3841 acagcggcgg ccccttcgtg ttgccggacg gtcgtgtcgc cggcgtcgtc ttcgcgaact
     3901 cggtggtggc cgaggatgtg ggctatgcga tcgtctcgac ccaggtgctg ccgagcgtga
     3961 aggaagcgaa ggcgagcgtc gaaccggtcg gcaccggctc gtgcaccaac tgacggtgcg
     4021 ttacgcctcg atcgacacct cgtacccaga ggcacgaaca ccgggccgga gcgccgtcca
     4081 gccgtcgccg ggggcgggca ccggacgagc gtctgtctcg aaggccagca gggacggacc
     4141 cgcgccggag acgcacaccg ggatgtcgcc ggcgcgcagc tcgttgaaca cgtcacggac
     4201 ggccgggacg agcgcgagcc gggcgtcctg atgcagccga tcctgcaagg ccactcccag
     4261 cagggacggg tcctgcgtca gcgcctccac cgccaaggcg gcatgagcgg cgttgaacac
     4321 cgcatcctcg cgggacacct tgggcggcaa ggcggctcgg gcttctttgg tcggcagccg
     4381 cgtggcaggc accaggacaa caggccgcaa cgacggatgg ggatcgagcc gccgcacgaa
     4441 cccgtcggag gccgcgatcg tgaacccccc gagggtggcc ggcgcggcat tgtccgggtg
     4501 cccttcgatg tcggctgcga gcgccaggat ccgctcgggc cgaagcgacg gtgacgcccc
     4561 cgccgccagg agatggtccg cggccaccac gccggccacg gcggccgccg acgaggagcc
     4621 gagcccgcgt tccagcggga tccggttgat gccgtgcacc gcgatcgccg gcaccgggga
     4681 tccgttcgcg gcgcacacgc tggcgatggt ggtgctcacc atgtcggtgc catcgacggg
     4741 gagttcgcga gccccctcgc cctcccacgt cacgccgggc tcggcgtcgg tgtcgatcat
     4801 cacctcgttg cacaggtcga gcgccaaccc gaacgaatcg aagccgggcc ccaggttcgc
     4861 cgaggtggcg ggcacgcgaa cggtgatgcg catcggctag aggcccagtt cggccgcgac
     4921 cgcggccgga tccggtttga ccacgaccgg gttggccgcg ccggcgatcg cccactccgg
     4981 atccttgagg ccgtggccgg tgaggatgca cacgacggtg gcaccgctcg ggagctggcc
     5041 gtcggcatgg agatggagca gacccgcaac gctggcggcg ctcgccggct cggcgaacag
     5101 gccctccctg gcgagccgcc gataggcggc caggatctcg cggtcggtca cggcgccgat
     5161 cgcaccctcg gactcggtgg cggcggccac cgctttgtcc cacgaggcgg ggttgccgat
     5221 ccggatcgcg gtcgcgatcg tctggggatc cttcacgacc tcaccgcgca cgatcggcgc
     5281 cgcgccctgg gcctggaacc ccatcaactg cggcggctcg gcgatcaggc cgtcggcgag
     5341 gtactccgaa tagcccagcc agtggctgga gatgttgccc gcgttgccga ccgggacgag
     5401 atgcatgtcg ggggcccggc ccaacgcatc gacgatctcg aacgcggcgg tcttctgtcc
     5461 ctgcaggcgg aacgggttga cggaattcac cagggtgacc ggatagcgct cggcgaggtc
     5521 cttggcgagc tcgagcgagt cgtcgaagtt tccttcgacc tccagcactc gggcaccgtg
     5581 caccagggtc tgggccatct tgcccaccgc gaccttgccc ttgggcacaa gcaccgcgca
     5641 ggtcaggccc gccttcgccg cgtaggccgc cgccgaggca ctggtgttgc cggtggaggc
     5701 gcacacgatc gccttcgcgc cttcctccag cgccttggag atggccaggg tcatgccccg
     5761 gtccttgaag gagcccgtgg ggttggctcc gtcgtacttc agccagactt cgcatccggt
     5821 ctcgtcggac aacggttcgg accgcaccaa tggggtgccg ccttcgcgga gggtgaccac
     5881 cggggttgcc tcactcaccg gcagacggtc gcggtactcc tcgatcagcc cgcgccactg
     5941 atgagccccc ggcatcggac cgtcggggct cacggctcct ccccttcgac gcgcagcacg
     6001 ctcgccaccg accgaacggc cgtggactca cgcaattcct gcagggtctt ctgcaatgcg
     6061 ccctcctgcg cccggtgcgt gatgaagacc agggtggctt cgtcggcgaa gccctcctgc
     6121 cacacccgct cgatcgagac gccgttgtga gcgaagtcgc ccgcgatctc ggccaggacg
     6181 ccgggacggt cggccacgtg caggttcagg tagtactggc cccgcgtgtc gtccatgggc
     6241 cggatgtgac gctcgagggc gcaggtgcag ccgatcgcgc ggccacccgt gacgaggttg
     6301 cgagcggccg agaccaggtc gcccacgacg gcggccgcgg tgggatcgcc gccggccccg
     6361 cgcccgtaca gcatcaactg ccccactttc gggccctcga tgaacaccgc gttgaacgcg
     6421 tcacggaccg aggcgagcgg atgcgcggcc gggatcatcg ccggatggac ccgggcgctg
     6481 atctcaccgt cttcgagttc ggcgatcgcg agcaacttca cgacgtagcc catgcgcgcg
     6541 gcgtcggcga tgtcctgggc ggtgatcgcg gtgatgccct cgcggtacac gtcgccggcc
     6601 accacccgcg agttgaacgc gatcgaggcg aggatcgcgc acttggcggc ggcgtcgaac
     6661 ccttcgacat cggcggtggg atcggcctcc gcgtacccca gcgattgggc ctcggccagg
     6721 gcctggtcga acgaccaccc gtgttcggac atctgcgtga ggatgtagtt ggtggtgccg
     6781 ttcacgatgc cgagcatgcg cgtgacgcgc tccccggcga gcgattcctt caaggaccgg
     6841 atcaggggga cgcccccggc caccgacgct tcgaagaaaa ggtcgcggtc ggcggcgtcg
     6901 gcggcctcga acagctccgc gccggcgttc gcgagcagtt ccttgttccc ggtgacgacc
     6961 ggcttgccgg cggcgaacgc ctccaggagg agaccgcgtg cgggctccag gccgccgatc
     7021 aactcgcaca cgacgtcgat gtcgggatcg gtgacgaccg aggcgccgtc ggtcgtgaac
     7081 gcctggcggg tcaagggaac gtcacggtcg agctcggggt cgcgtaccgc aaccctggtc
     7141 acctcgacgc ggcagccggc acgcaaggcg atgtcgtcgg cgtgctcgtg cagcatgcgg
     7201 atcaccgcgg cgccgacggt gccgcagccc agcatgccca ccctgatcgt gcgttcgctc
     7261 atggccggtc cgttccctcg agatcctcgc ccgcgatcca ggccgccatc agtatctcgt
     7321 cctcgtcacc gtaggacttg cgcgactgcc gcgacagccg gccctcctgc acgaagccga
     7381 acttgcggta gagcgcgatc gcccccgtgt tgtgcgggta gacggacaag acgagcttct
     7441 ccacgcccac accgcgggcc cacacgatgg cgtcggcgag gagcgcgctc ccgacgccac
     7501 ggcctcggtg gtcggcggcg acggcgatcc ccagggtggc cacgtggtgg gtcaccgggt
     7561 gtcgttcccg ctggatgtag atgtggccga cgaccgtgcc ctgcccgtcg accgccacga
     7621 tctgtgcctc gtgatcggtc cacgggcgcc ggaaccgcga ccggtacacc cgcttggagt
     7681 ggctcacgcg ctcggtgcgc acgtaccggc cctccgccac cacggccgtc cagaaggcga
     7741 tgtaggaatc ggcgtcgcgt ggagcggccg gccggatcgt gatgctctcg ggcgcctcgg
     7801 cgacgacggc cgcccgcggc gcaggaccct cgaggcgatc caggtcggcg gcatcctccc
     7861 ggcggagcca gaggcgcgcg agcccgtcac gcacccccac caccgcggga cggccgacgc
     7921 ggttgtagct gctcgcgagc gagtacgtgt aggcgccggt cgccgcgaat gcgagcaggt
     7981 cgccgggccc ggcgtcggcg ggcaggtgca cgtcctcggc gagggtgtcc ccggactcgc
     8041 agtgccggcc cacgacggtg aaggtcgcga ggtcttcccc ggcgctcggg cccacggccg
     8101 ccacggtgta tcgcgcgtcg tagagcatgg gccggacgtt gtcggacatg ccgccgtcca
     8161 ccgccaacag cgtccgcccg ttccccacct gcttgcgtga tcccacccga taggcggtga
     8221 gccctgcgtt cgccacgatc gaacggccgg gttcgacgac gagcgagggc accggcagat
     8281 ccagggcggc gcatcgctcg gagagccgag cggtcaccgc ggcggcgagc gcgtcggtgg
     8341 ccagcgggcg ctcgtccacg tacgtgacgc cgaacccgcc gccgagatcg aggaccgcgg
     8401 ccgtcgcgcc cacccggtcg cggatgtccg cgagcgtctg caccagccgg tccagcgatc
     8461 gcaggtaggg ctcgaggtcg agcacctgcg atcccacgtg cgcatggatc ccgtcgaacc
     8521 ggatccccgt catcgagacc gccgcctcga tcacaccggg cgcgtcggcg agcgacatgc
     8581 cgaacttcga cgcatcgtga ccggtggcga tcgcctcatg cgtcgccacc tccacctcgg
     8641 gcaccacgcg gagcaggacc cgctgcacgc ggccctgcga gcgggcgatc gcatcgagcc
     8701 gttcgagttc ggcctggtgg tcgacgacga cgagcgagac acccgaaccc accgcgagcg
     8761 ccagttcctc gtcggtcttg ttgttgccgt gcagcacgat gcgggaggcg ggcgctcccg
     8821 cccgcaggca cgcctccacc tcgccgccgg tcgaggccag gaggtcgagg ccttcgtcca
     8881 gcacgatccg cagcacggcg tgggcggtga acgccttcac ggcatagagc acgcggggga
     8941 accgcccggc gatcgcgcag gcacgatcgc ggacctcctg ctcgtccacc acgagcagcg
     9001 gcgtgccgaa ccgggcggcc agctcggtga gggcgaggcc gcccacctcg agcccccacg
     9061 gcccacgcag agcggtggcg ggccacggcg gcgccggcag ttcgtcggct gccgatggac
     9121 gcggcgacgg ctcagccatc gccggtgcgc tccatcgact ccggcgccga gacgccgagg
     9181 atgccgagga ggttcgcgat gacctgcttc gtgccggcgg cgagccacag gcgggcgtgg
     9241 gtcagcgcgg gttcgtccgc gttcaccacc gtgcactcgg tatagaaccg gtggaaccgg
     9301 ccggcgagat cctgcgcgag atgggtgatg cgctgtggtg cgcgcagctc ggcggcggcg
     9361 gcgacctggc cgggcacgtc ggcgagcgcg cgcagcaggt cgagctcggc ctcgtgccga
     9421 agcagggaca ggtcggcgtc ggcgatggac gtgcgcgcga cgcccaccga ctcggccttt
     9481 cgcaggatcg aggcgatccg cgcgtgtccg tactgcacgt agtagacggg gttgtccatg
     9541 gactggcgct tcacggtctc gatgtcgaag ttcatcgccg aatcgcccga gaacgacagc
     9601 agcgtgaacc gtgcggcatc ggcgcccacc tcatcgatca gctcgtcgag ggagatgaac
     9661 gtgccggccc gcttgctcat cttcacgggc tcgcccccgc gctggaacga cacgaactgg
     9721 tacagcacga tctcgagcgc gtccgggtca tagcccatcg cggatgcggc gcccttcatc
     9781 cgggccacgt cgccgtggtg gtcggcgccc cacacgtaga ccacgtgatc gaagccgcgt
     9841 gcgaacttgt cgacgatgta ggcgcagtcc gcgccgaagt aggtgtgcgt cccgttggac
     9901 cgcacgatca cccggtcctt gtcgtcgccg aaggccgtcg agcggaacca ggtggcgcca
     9961 tcggcgtcgt aggcgtagtc ggcggcgcgc agccgctcca ccgccgccgc gatctcgcct
    10021 ttgcgctcga gctcggcctc ggagaagtag acgtcgaacc gcacgccgaa ccgctcgagg
    10081 gtctgctcca cgccggccag gatccgacgg gtgccctcgg cgagcagccg ggcttcgcgc
    10141 tcgggttcgg gcaggtcggc gagggcgtcc ccgtgctcgg ccacgatgtc gcgggcgacc
    10201 tccaccacat actccccgtg atagccgtcc tccggcagct ccgcctcccg gcccagcacc
    10261 tgcaggtagc gcgccaccac cgaggcgccg aacaggtcca tctggcggcc cgcatcgttg
    10321 aagtagtact cccgctcgac ggtctcgccg gcggcttcca gcaggcgggc gagcgcatcg
    10381 cccagcgcgg cgttgcgggc atgcccgaca tggagcggcc ccgtggggtt ggcgctcacg
    10441 aactcgacct ggaccctgcg ccccgtgggt tcggcgcggc cgtaggcctc gccgaggtcg
    10501 gcgatcgaac gcagcgcgtc gtgcagccac gtgtcggccg tgaagatgtt gaggaacccc
    10561 ggcccggcca cctccacctt ctcgaccaac ggcgaggcgg gcagtgcgtc ggcgatcgcc
    10621 tgcgccacgt cgcgaggagg acggccggcc cgcttggcta gcgcgagcgc cacgttggtg
    10681 gcgaagtccc cgtgaccctt ctgcttggga gccagcaacc cgggcgtggg caggtcgccc
    10741 tcgatgccca gcgcgggggc cgcctcgcgc aggccgtcct ggagccagtc gacgagct
//
LOCUS       DATDBZ010000392         7539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_754377, whole genome shotgun sequence.
ACCESSION   DATDBZ010000392 DATDBZ010000000
VERSION     DATDBZ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754377"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VIX62_09350"
     CDS             <1..568
                     /locus_tag="VIX62_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860476.1"
                     /translation="IVTVITAIAAVALSLNRLRTLMWLGLASAIALIVARRAAIRSQD
                     DIVNLAPNGSNRGAVAATVSRVLGNLRGFTTLLLVATVVIAVLAYLASRPSWLSRAVE
                     RAGEGNWLDRENVVVRWIADHTQVLRIVSIGIVALFLLFSDVSWGVFGIVLVLLVAWL
                     LVLQVLQPSRPVAPASPETPAAPATPDA"
     gene            632..1150
                     /gene="msrA"
                     /locus_tag="VIX62_09355"
     CDS             632..1150
                     /gene="msrA"
                     /locus_tag="VIX62_09355"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY5860477.1"
                     /translation="MTETAIFGAGCFWGVEWVFRQVPGVQDAVSGYAGGLTDNPTYRD
                     VCSHTTGHAEVVEVTFDPSVVTFEQLLEVFWAMHDPTQVNRQGPDVGDQYRSVVFTTT
                     PEQLAIAVASKERAQARFDKPIATQIEEAPTFYPAEGYHQRYYEGNGHTPYCHVLPVG
                     VLKDLGLVPASS"
     gene            complement(1166..1837)
                     /locus_tag="VIX62_09360"
     CDS             complement(1166..1837)
                     /locus_tag="VIX62_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY5860478.1"
                     /translation="MDEGSEPRIHVTDGPYVVEGRLPLVRTSIVQTDHGEPIGWAPDQ
                     PVESREELKLCRCGHSSDKPFCDGTHRTIGFQGAETADRGPIAARRKTYAGDGVVMTD
                     DRSICEHAGFCGDRFTNVWRMIKDTDDPAVRERLIDMVRLCPSGALAASEEEDGEPME
                     PALPTSVAVVEDGPLWVRGGVRVTGADGQDYEVRNRVTLCRCGASTNKPFCDGTHQEI
                     GFTDA"
     gene            1940..2938
                     /locus_tag="VIX62_09365"
     CDS             1940..2938
                     /locus_tag="VIX62_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860479.1"
                     /translation="MARSIRARSLLVTAAGIAAITVSVVPASASFPDQGPRVLFARAK
                     MPPARTLSPLRGIGALDLFTMKPNGDDQVRVVKTDALLEYGGHWSPNGARMTFAGSKG
                     KGNLDIYTAKADGTQRKQLTTAATNYSPNWSKNGQQIVWTRLGGTFAGRRLSPATLRG
                     IDANLMVMNADGTDKHSIFTGFAMAPAWNPTAGRIAFSTYDGDSLDVSWIKPNGLGLQ
                     QLTDFGIGTFTLFGDWAPDGDSFAMLFDGGMARGGIDGYDLYLGTPEGTKPIFLAADA
                     DLEFSPVFTADGSRVIFIRHDGSDFELFSVKADGSGGEKQLTTNGADDLLSLVPLI"
     gene            2981..5014
                     /locus_tag="VIX62_09370"
     CDS             2981..5014
                     /locus_tag="VIX62_09370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY5860480.1"
                     /translation="MFGDQLNDAQRAAATHGDGPLLIVAGAGTGKTATLAARVAWLIE
                     QGTRPERILLLTFSRRGAREMLSRAERMTGADAGRVWGGTFHAVGNRLLRLHGRPLGL
                     RPDFTVLDQADGADVMNLLRDEFGFSSRERRFPRKETLASIYSRTVNAGDKLGDVLKR
                     HYPWCLEEADGIREIFTAYTVRKREQNVLDYDDLLLFWKALAWSDATREPMAAMFDHI
                     LVDEYQDTNALQADILEGMRPPSARNLTVVGDDAQSIYGFRAATVRNILEFPQRFDGA
                     TVITLEQNYRSIPPILAASNAAIALSPQRHDKTLWSARRGDRTPTLRTCLDEPAQSAA
                     VCDTVLAHREEGVPLKEQAVLFRAAHHSDLLEVELARRSIPFVKYGGLRFMEAGHVKD
                     TLAILRVLENPWDEVAWFRVLQLPEGMGPATARRLMTQIGVRDGNPDEASPLVKVLDQ
                     PVDVPRAAVEGFQELRSALRGCVDETVLPPAAQLERLRPFLDEVIGRTYPAAAARTRD
                     LDQLTLLAQGYESRGRFLSELTLDPPSSTGDLAGPPLLDEDYLVLSTIHSAKGLEWDV
                     VHLIHAADGMIPSDMATGDDDEIEEERRLLYVALTRARDALHVTFPMRYYRRPKGLED
                     PHSYAQLSRFLEPASVASCFEPIAPAIEPAPDAVVDLTGTADVDGFLAGLWAE"
     gene            5036..5710
                     /locus_tag="VIX62_09375"
     CDS             5036..5710
                     /locus_tag="VIX62_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5860481.1"
                     /translation="MPTYDAVLLDLYDTLVWSDWLAWQHTLAERLGITPQAMGEVFTV
                     TRPARSKGENADENADMVAIVEAAGHAPDPDLVAELIALEGEMMRDRVHLYDDSLDVV
                     RGLRARGVKTALVSNCSYNTVPIVERLGLEAEFDAVILSFAVRSMKPEPEIYRLALEA
                     LGDPAPSRAVFVDDQVLYCDGAKAVGLDTYLIFRPEEAMEGRPANTNGHCIIEDLRPL
                     IAETLG"
     gene            complement(5707..6408)
                     /locus_tag="VIX62_09380"
     CDS             complement(5707..6408)
                     /locus_tag="VIX62_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY5860482.1"
                     /translation="MSDVDPRILADRKQLRAVPIHERPKPRMRGRLHQAAFFAAIPAG
                     LVLVALAPTGRARIATLIYALTLIGLFGVSASYHLGAWSEEAYVRMRRLDHSMIFVLI
                     AGTYTPFCMLVLTGALGTATLITVWAGAAVGVATKFYRVDLHVLSGFMYIGLGWVAVV
                     SLPALIRGLDTPETVLLIAGGLLYSFGALVLATNKPNPWPLTFGYHEVWHSITIAAAI
                     CHYLCILMIVLQLRG"
     gene            6473..7321
                     /gene="ppk2"
                     /locus_tag="VIX62_09385"
     CDS             6473..7321
                     /gene="ppk2"
                     /locus_tag="VIX62_09385"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY5860483.1"
                     /translation="MTEAMERQGSGRTKFPNKAYEKELYRLQEELVKMEQWVAETGAR
                     VVVIFEGRDAAGKGGTIKRIAEHTNPRITKHVALPKPTERQTSQWYFQRYVAELPAAG
                     EIILFDRSWYNRAGVEHVLGFCTPNEYYRFLRQCPIFERLLVEDGIILIKYWFSVSDE
                     EQERRFAKRITDPLRRWKLSETDLYSRTKWVDYSRAKDEMFVHTDLPEAPWYVVESDT
                     KKKARLNCISHLLSKIPYQEEAVPEIELPDRQKDEGYVRPPRDLYTYVPDHADEIAAE
                     HHGKDD"
BASE COUNT         1282 a         2584 c         2530 g         1143 t
ORIGIN      
        1 catcgtcacg gtgatcaccg cgatcgcggc cgttgccctg agcttgaacc ggctgcggac
       61 gctgatgtgg ctggggctgg cctcggcgat cgcgctcatc gtcgcgcgac gtgccgcgat
      121 ccggtcccag gacgacatcg tcaacctggc gccgaacggc tcgaaccgtg gggcagtggc
      181 ggccaccgtc tcgagggtcc tggggaacct ccgtgggttc accaccctcc tgctggtggc
      241 cacggtcgtg atcgccgtcc tggcctatct cgcgagccgt ccctcgtggc tctctcgcgc
      301 cgtcgagcgg gcgggcgagg gcaactggct cgaccgcgag aacgtcgtcg tgcggtggat
      361 cgccgatcac acccaggtcc tgcgcatcgt gagcatcggc atcgttgccc tgttcttgct
      421 gttcagcgac gtgagctggg gggtgttcgg catcgtgctg gtgctcctgg tggcctggct
      481 cttggtgctc caggtgctgc aaccgagcag acccgtggcc cccgcgtcac ccgaaacccc
      541 cgcggcaccc gcaacacccg acgcgtaacg ttccggcccg ttcgggggac gatggagcag
      601 ttccatcgat cacgaggagg cgaacacgac catgaccgag accgcgatct tcggcgcggg
      661 atgcttctgg ggcgtcgaat gggtgttccg gcaggtgccg ggcgtccagg atgccgtcag
      721 cggctacgcc ggcggactca ccgacaaccc gacctaccgc gacgtctgca gccacaccac
      781 cggacacgcc gaggtggtcg aggtgacctt cgacccgtcg gtcgtcacct tcgagcagct
      841 gctcgaggtg ttctgggcga tgcacgatcc gacccaggtg aaccggcagg gcccggacgt
      901 gggtgaccag taccgcagcg tggtcttcac cacgaccccg gagcagctcg ccatagccgt
      961 cgcatcgaag gagcgggcgc aggcgcggtt cgacaagccg atcgcgaccc agatcgagga
     1021 ggcgcccacc ttctacccgg ccgagggcta ccaccagcgg tactacgagg gcaacgggca
     1081 caccccgtac tgccacgtgc tgccggtcgg tgtcctgaag gacctggggc tggttccggc
     1141 ctcgtcgtga ggcccccggc cccgctcagg cgtcggtgaa cccgatctcc tggtgggtgc
     1201 cgtcgcagaa gggcttgttg gtcgaggcgc cgcagcggca cagcgtcacc cggttgcgca
     1261 cctcgtagtc ctgaccatcg gctccggtga cccggacccc accgcgcacc cacagcgggc
     1321 cgtcctccac gacggccacg ctggtgggga gcgcgggttc catcggctcg ccgtcttcct
     1381 cctcgctcgc cgcgagggcg ccggacgggc acaggcggac catgtcgatc agacgttcac
     1441 gcaccgcggg gtcgtcggtg tccttgatca tgcgccagac attcgtgaac cggtcgccgc
     1501 agaacccggc gtgctcacag atcgagcggt cgtcggtcat gacgacccca tcgcccgcgt
     1561 aggtcttgcg gcgggctgcg atcgggccgc gatcggcggt ctcggcgccc tggaagccga
     1621 tcgtgcgatg ggtgccgtcg cagaacggtt tgtccgagga gtggccgcat cggcacagct
     1681 tcaactcctc gcgtgattcc acgggctgat ccggcgccca gccgatgggc tcaccgtgat
     1741 cggtttgcac gatcgaggtc cgcaccagcg gcagccggcc ttcgaccacg tacggtccgt
     1801 ccgtgacgtg gatgcgaggt tcggaaccct catccatgtg tggagcctac cggggtcccg
     1861 ggcgaaggcg tcgacgctcc acggtcggcg ggggaggccg tgggatactc gcgccgttcg
     1921 tccgagagga gccccagcca tggcccgttc gatccgcgcc cgatccctgc tcgtcaccgc
     1981 cgccgggatc gcggccatca ccgtctccgt cgtccccgcg tcggcgagct tccccgatca
     2041 ggggccgcgg gtgttgttcg cgcgggcgaa gatgccgccg gccaggacgc tcagcccgtt
     2101 gcgagggatc ggcgcgctcg acctgttcac catgaaaccg aacggcgacg accaggtgcg
     2161 ggtcgtcaag accgacgccc tcctggagta cggcggccac tggtccccca acggcgcccg
     2221 catgacgttc gccggctcga aaggcaaagg caacctcgac atctacacgg ccaaggccga
     2281 cggcacgcaa cgcaagcaac tcacgaccgc ggcgacgaac tacagcccga actggtcgaa
     2341 gaacggccag cagatcgtgt ggacccgtct gggcggcacg ttcgccggtc ggcggctctc
     2401 acccgcgacg ctgcgaggga tcgacgcgaa cctgatggtg atgaacgccg acggcaccga
     2461 caagcattcg atcttcaccg ggttcgcgat ggcgccggca tggaatccca ccgcgggccg
     2521 gatcgcgttc agcacctacg acggtgactc gctcgacgtc tcatggatca agcccaacgg
     2581 tctgggcctc cagcagctga cggacttcgg catcgggacc ttcaccctgt tcggcgactg
     2641 ggcaccggac ggggactcgt tcgccatgct cttcgacggc gggatggccc ggggaggcat
     2701 cgatggctac gacctctatc tcggcacgcc cgagggcacg aagccgatct tcctggctgc
     2761 ggacgcggac ctggagttct ccccggtgtt caccgcagac ggctcgcggg tgatcttcat
     2821 ccgtcacgac gggtccgact tcgagctgtt ctccgtcaag gccgacggca gcggcggcga
     2881 gaagcagctc accaccaatg gcgcggacga cctcctcagc ctcgtcccct tgatctagcc
     2941 ctcgcccccc ggggaggaac cgggtataca ccccgaacac atgttcgggg atcagctcaa
     3001 cgacgcgcag cgagctgcgg ccacccacgg cgacggtccg ttgctgatcg tcgccggcgc
     3061 gggcaccggc aagaccgcca cgctcgccgc tcgggtggcg tggctgatcg agcagggcac
     3121 gcgccccgaa cgcatcctgc tgctcacgtt cagccggcgt ggcgcccgcg agatgctctc
     3181 ccgcgccgag cgcatgaccg gggccgacgc gggaagggtg tggggaggca ccttccacgc
     3241 cgtgggcaac cggctgctcc ggttgcacgg caggccgttg ggattgcgtc ccgacttcac
     3301 cgtgctcgac caagccgacg gcgccgacgt gatgaacctg ttgcgcgacg agttcggctt
     3361 cagcagccgt gagcgccgat tcccccgcaa ggagacgctc gcatcgatct actcgcgcac
     3421 ggtcaatgcc ggcgacaagc tcggtgacgt gctgaagcgt cactacccct ggtgcctcga
     3481 ggaggccgac ggcatccgcg agatcttcac cgcctacacc gtccgcaagc gcgagcagaa
     3541 cgtcctggac tacgacgacc tgttgctgtt ctggaaggcg ctggcgtgga gcgatgccac
     3601 ccgggagccg atggcggcga tgttcgacca catcctggtc gacgagtacc aggacacgaa
     3661 cgcgctgcag gcagacatcc tcgagggcat gcggcctccc agcgcgcgga acctcacggt
     3721 ggtgggcgac gacgcccagt cgatctacgg gttccgagcg gccacggtgc gcaacatcct
     3781 ggagttcccg cagcggttcg acggcgccac cgtgatcacc ttggaacaga actatcggtc
     3841 gatcccgccc atcctcgccg cttccaacgc cgcgatcgcg ctcagccccc aacgccacga
     3901 caagacgctg tggtcggcgc ggcgcggcga tcgcacgccg accttgcgca catgcctgga
     3961 cgagcccgcc caatccgccg cggtgtgcga caccgtgctc gcccatcgtg aggaaggggt
     4021 gccgctgaag gaacaggcag tgctgttccg agcggcccac cacagcgatc tgctcgaggt
     4081 cgaactcgcc cgtcgcagca tcccgttcgt gaagtacggc ggtctgaggt tcatggaagc
     4141 cggacacgtg aaggacacgc tggcgatcct gcgggtgctg gagaatccgt gggacgaggt
     4201 cgcgtggttc cgcgtgctgc agctaccgga gggcatggga ccggccaccg cacgccgcct
     4261 gatgacccag atcggcgtgc gcgacgggaa cccggacgag gcttcgccgt tggtcaaggt
     4321 gcttgaccaa cccgtcgacg tgcctcgagc ggcggtcgag ggcttccagg agctccggtc
     4381 agcgctccgt ggctgcgtgg acgaaaccgt gctacctccc gccgcccagc tggagcgcct
     4441 gcggccgttc ctggacgagg tgatcgggcg cacctacccc gcggccgccg cgcggacgcg
     4501 tgacctcgac cagctcacgc tgctggccca ggggtatgag tcccgcgggc ggttcctgtc
     4561 ggagctgacc ttggatccgc cgtcgtcgac cggcgacctc gccggtcctc cgctgctcga
     4621 cgaggactac ctggtgctct cgacgatcca ctccgccaag gggctcgaat gggacgtcgt
     4681 ccacctgatc catgccgccg acggcatgat cccctccgac atggcgaccg gcgatgacga
     4741 cgagatcgag gaggagcgcc gactgctgta cgtggcgctc acccgcgccc gggacgccct
     4801 ccacgtgacg ttccccatgc gctactaccg ccgtccgaaa gggctcgagg acccgcacag
     4861 ctacgcgcag ctcagccggt tcctggaacc ggcctcggtg gcatcgtgct tcgaaccgat
     4921 cgctccggcg atcgagcctg cgcccgatgc cgttgtggat ctcacgggca cggccgacgt
     4981 cgacgggttc ctcgccggcc tctgggccga gtgacgcgcc ggtaggctgg gacccgtgcc
     5041 cacctacgac gcggtcctgc tcgacctgta cgacaccctg gtgtggagcg actggctggc
     5101 gtggcagcac acgctggccg aacggctcgg catcacgccc caggccatgg gcgaggtgtt
     5161 caccgtcacg cgcccggcca ggagcaaggg cgagaacgcg gacgagaatg ccgacatggt
     5221 cgccatcgtc gaggcagcgg ggcacgcgcc cgaccccgac ctggtagccg agctcatcgc
     5281 gctggagggc gagatgatgc gtgatcgcgt gcacctctac gacgattcac tggacgtcgt
     5341 gcgcggcctt cgtgcgcgcg gcgtgaagac cgcgctcgtg agcaactgct cgtacaacac
     5401 ggtccccatc gtcgagcgac tggggctgga ggcggagttc gatgccgtga tcctgtcgtt
     5461 cgccgtgcgg agcatgaagc ccgaacccga gatctaccgc ctggccctgg aagccctcgg
     5521 cgaccccgcc ccgagtcgtg cggtcttcgt cgacgaccag gtgctctact gcgacggcgc
     5581 gaaagcggtg ggtctggaca cgtacctgat cttccggccc gaggaggcca tggaaggccg
     5641 tcccgccaac acgaacggcc actgcatcat cgaggacctg cgaccgctga tcgccgagac
     5701 gctcggctag ccgcggagct gcagcacgat catcaggatg cagaggtagt ggcagatcgc
     5761 cgcggcgatc gtgatggagt gccacacctc gtgatagccg aacgtcaacg gccacgggtt
     5821 gggcttgttg gtggccagca cgagcgcgcc gaaggaatag agcaggccac ctgcgatcag
     5881 caacaccgtc tcgggcgtat cgaggccgcg gatcaaggcc gggaggctga ccaccgccac
     5941 ccagcccagc ccgatgtaca tgaacccgga cagcacgtgc agatcgacgc gatagaactt
     6001 cgtggccacg cccacggcgg ctcccgccca cacggtgatc aaggtcgcgg tgcccagcgc
     6061 ccccgtgagc accagcatgc agaacggtgt gtaggtcccc gcgatgagca cgaagatcat
     6121 cgagtggtcg aggcggcgca tccgcacgta ggcctcttcc gaccaggccc ccaggtggta
     6181 ggacgcgctg acgccgaaca acccgatcag ggtgagcgcg tagatgagcg tcgcgatccg
     6241 cgcacgcccg gtgggtgcga gcgccaccag caccaggccc gccgggatcg cggcgaagaa
     6301 cgcggcttgg tggagccggc cccgcatgcg gggcttcggg cgctcgtgga tcgggacggc
     6361 gcggagctgc ttacggtcgg ccaggatgcg gggatcgacg tcggacaccc acccagccta
     6421 gagcacacgc ccctagactc atcgcacagc gcgacgtctg gaggcggatg acatgaccga
     6481 agcgatggag cggcagggtt cgggccgcac gaagttcccg aacaaggcct acgagaagga
     6541 gctctaccgg ctgcaagaag aactcgtgaa gatggagcag tgggtcgccg agacgggcgc
     6601 tcgcgtcgtg gtgatcttcg aaggacgaga tgcggccggc aagggcggca cgatcaaacg
     6661 gatcgccgaa cacacgaacc cgcggatcac gaaacacgtc gcgctgccca agcccaccga
     6721 gcgtcagacc tcccagtggt acttccagcg atacgtggcc gagctacccg cggcgggcga
     6781 gatcatcctg ttcgaccgca gttggtacaa ccgcgccggc gtcgagcacg tgctcgggtt
     6841 ctgcacaccg aacgagtact accggttcct ccgccagtgc ccgatcttcg aacgcctcct
     6901 ggtggaggac ggcatcatcc tgatcaagta ctggttctcg gtgagcgacg aagagcagga
     6961 gcgccggttc gccaagcgca tcaccgatcc gctgcgccgc tggaagctga gcgagaccga
     7021 cctgtactca cgcaccaagt gggtggacta ctcgcgggcg aaagacgaga tgttcgtcca
     7081 taccgacctg cccgaggcgc cgtggtacgt ggtcgagtcc gacacgaaga agaaggcccg
     7141 gctcaactgc atctcgcacc tgctctcgaa gatcccgtac caagaggaag ccgtgccgga
     7201 gatcgagctg ccggaccgcc agaaggacga gggctacgtg cggccgccgc gtgatctcta
     7261 cacctacgtg cccgaccacg cggacgagat cgccgccgag catcacggca aggacgactg
     7321 agttccgctc cgggtcgatc gaggcgagga cgagttggga cgccgacggg tgctcgacca
     7381 acgatcactc gatcggtgac gaccgaggtt ggacaagcga gcaaccgggc agcatgatgc
     7441 cgatgttgcc cgaacgcagc cagcgaaggg actggtgtgt tcacgccaag cgttgctcgg
     7501 ggtctcgttg tcgccctcac attggcaacg gttgtggcg
//
LOCUS       DATDBZ010000393         3089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2789561, whole genome shotgun sequence.
ACCESSION   DATDBZ010000393 DATDBZ010000000
VERSION     DATDBZ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2789561"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VIX62_09390"
     CDS             complement(<1..305)
                     /locus_tag="VIX62_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEY5860484.1"
                     /translation="MAERTPNRLAGETSPYLLQHANNPVDWFPWGGEALSRAAERDRP
                     IFLSIGYAACHWCHVMERESFEDDVTAQLMNDRVVSIKVDREERPDLDGIYMDAVQA"
     gene            389..1054
                     /locus_tag="VIX62_09395"
     CDS             389..1054
                     /locus_tag="VIX62_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860485.1"
                     /translation="MADSIRVLVVDESPGLTQGLILSLPRHGPVYVLGPVPDAPEALA
                     ALEEGLADMVLVDLDRADGRGAEVVGAIRDGGGRVRVLAASGHTGGRDDPEVIAMALA
                     AGACGVLPSERDRSLIDVFRRAFAGELVLPAADLPRLVDRLVSSNGSHGLTSLTARER
                     QILCSLADGGSTVEIATTLSISPLTVQSHVKSILAKLGVHSKVEAVRLAWRHGLGSSS
                     RSA"
     gene            1056..1727
                     /locus_tag="VIX62_09400"
     CDS             1056..1727
                     /locus_tag="VIX62_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860486.1"
                     /translation="MDTAAIGPVGVTIAIDHPLVRDAVRRSCDRVGTVQVLAEVADAP
                     ALLAACRDRAPAVIVLDDELGGGHGLEALRAVREQGFAAGVLVLTDRTDGASVLDALK
                     LGVRGYLGKADGLRGVGDAVSRIAAGERLIDPELEQAAVMALGEFARHAREGSEVQAT
                     LTPREQQILVLVSDGLTMQQVASRLGISPRTVETHVAKLYRKLGVRTRVQAVSRAAQL
                     GLIDL"
     gene            1792..2496
                     /locus_tag="VIX62_09405"
     CDS             1792..2496
                     /locus_tag="VIX62_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860487.1"
                     /translation="MVTREIEHPNERRRRRLGVVVMDPLPVVRAGLRLLIDDRPDMEV
                     LEEAGTADAGLELVSRVRRNRVVVIVGLGISGEHDAFWLIRTLRERFPSHAILGCGAN
                     SDPTTISRALFMGADGFVDKNIDPVEFLQSLRRVADREMVLASPTPGSVAQIAEGIER
                     RRDLDVRLTEREREVLRVAAEGLTAREIAVRLGVRERTITTHLARIYGKLGVGNRLAA
                     IRIAARSGLVSVGSPE"
     gene            2669..2881
                     /locus_tag="VIX62_09410"
     CDS             2669..2881
                     /locus_tag="VIX62_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY5860488.1"
                     /translation="MFLRIYTGATTWWDGLKTRAAEEGGATAVEYALMVALIAVVIIA
                     AVAALGQSSSEQFTTVGDCVADPNAC"
BASE COUNT          478 a         1003 c         1078 g          530 t
ORIGIN      
        1 atcgcctgga cggcgtccat atagatgccg tccaggtccg gtcgttcctc ccgatcgacc
       61 ttgatcgaga cgactcgatc gttcatcaac tgggcggtca cgtcgtcctc gaaggactca
      121 cgctccatca cgtggcacca gtggcacgcg gcgtagccga tcgacaggaa gatgggcctg
      181 tcgcgctccg ccgctcgcga gagagcttcg cccccccaag ggaaccagtc gaccgggttg
      241 ttcgcatgct gcaacaggta cggactggtc tcgccggcca gtcggttcgg ggtgcgctcg
      301 gccatgggac cagtgtctct cgcaaccggc ggagcgactt cgccgcctcc ttcaggcggc
      361 gggctgcgcg tgccgatggt tggcggagat ggccgactcg atccgtgtgc tcgtcgtgga
      421 cgaaagccct ggtctgacgc aggggctgat cctctccctt ccccgtcatg ggccggtcta
      481 cgtcttgggg ccggtacccg acgcccccga agccctcgca gcattggagg agggcctcgc
      541 tgacatggtc ttggtagatc tcgatcgagc cgacggccga ggcgccgaag tggtgggggc
      601 gatccgagac gggggaggca gggtccgcgt gttggccgcc agcggtcaca ccggcggtcg
      661 agatgatccc gaggtcatcg ctatggcgtt ggcggcaggg gcgtgtgggg tgctcccgtc
      721 cgagcgtgat cgatctctga tcgatgtgtt ccggcgtgcg ttcgccggcg agttggtcct
      781 cccggcggca gacctgccgc gtctggtcga tcgtctggtg tcgagcaatg ggtcccatgg
      841 actcacctcg ctcaccgccc gcgagcgcca gatcctctgt tcgctcgccg atggcggctc
      901 gaccgtggag atcgccacca cgctatccat cagcccactc accgtgcaga gccacgtcaa
      961 gagcatcctg gccaagctgg gtgtccattc caaggtcgag gcggtgcggc tggcatggcg
     1021 tcatgggctg ggctcgtcgt cacgaagcgc ctgacgtgga caccgccgcc atcggtcccg
     1081 tcggggtcac gatcgcgatc gaccacccgc tggtccgcga tgccgtgcgc cgttcgtgcg
     1141 atcgggtcgg caccgtccag gtcctggctg aggtcgcgga cgcgcccgcg ttgctcgccg
     1201 cttgccgcga ccgagcgccc gcggtgatcg tcctcgacga cgagctgggt gggggacatg
     1261 gcctcgaggc cctccgcgcg gttcgcgaac aggggtttgc cgcgggcgtc ttggtgctca
     1321 ccgaccgcac cgatggcgct tccgtgctgg acgcgctcaa actcggtgtg cgtggctacc
     1381 tcggcaaggc tgacggcctt cggggcgtcg gcgacgccgt gtctcggatc gcggccgggg
     1441 aacgactgat cgaccccgag ttggagcagg cggcggtgat ggcgttaggg gagttcgcgc
     1501 gccatgcgcg tgaaggttct gaggttcagg cgaccctcac cccccgcgaa cagcagatct
     1561 tggtgctggt gagcgatggg ctcacgatgc aacaggtcgc gagccggctg ggcatctcgc
     1621 cgcgaaccgt ggagacccac gtcgcgaagc tctaccgaaa actcggcgtg cgaacccggg
     1681 tgcaggcggt atcgcgggcc gcccagctgg gtctcatcga cctctaggcc ggcacggacg
     1741 gggcgcgacg cgtcgattca gccggtgcgt gccaggtgcc gatagaggac cgtggtcacc
     1801 cgcgagatcg agcatcccaa cgaacgacga cgacggcgtc tgggcgtcgt cgtgatggac
     1861 ccgctgcccg tggtccgggc cggcctgcga ctgctgatcg acgatcgccc cgacatggag
     1921 gtgctcgaag aagcagggac cgccgatgcg ggactggagt tggtcagccg ggtccggcgc
     1981 aatcgtgtcg tcgtgatcgt cggcctgggg atcagcggcg agcacgatgc gttctggctg
     2041 atccgaacgc tgcgcgaacg cttccccagc cacgccatcc tgggctgtgg cgccaactcc
     2101 gatcccacca cgatctcgcg ggcgttgttc atgggcgcgg acgggttcgt ggacaagaac
     2161 atcgacccgg tcgagttcct gcagtcgctg cgccgtgtcg ctgaccggga gatggtcctc
     2221 gcgagtccaa cgccgggctc ggtggctcag atcgcggaag ggatcgaacg ccgtcgcgac
     2281 ctggatgtgc gactgaccga acgggagcgc gaggtgttgc gggtcgccgc ggaggggttg
     2341 accgcgcgcg agatcgccgt tcgtctcggg gtccgcgagc ggaccatcac gacccacctc
     2401 gcccgcatct acggcaagct cggcgtcgga aacaggctcg ccgcgatccg catcgctgcc
     2461 cgatctggct tggtctcggt ggggtccccc gagtgacctg cacgcacatg cgtacgtagg
     2521 actacgtagc caccctccgt gaaacacgga attccctggt cagagggcac aacttaccgt
     2581 ggtcgagcgc actcaagctt tcgcgccgag gggccgaaaa ccccttcgat aggacgggat
     2641 ctctttgaaa ggaagggggt gcaacaacat gttccttcgc atctacaccg gtgcgaccac
     2701 ctggtgggac gggcttaaga cccgcgctgc tgaagagggt ggtgcaaccg cggtggagta
     2761 cgcgctgatg gtggccctga tcgctgtggt catcatcgcg gcagtggcgg ccctcgggca
     2821 gagctcgagc gagcagttca ccacggtcgg cgactgtgtg gccgacccga acgcctgctg
     2881 atccaccgat cgacagcacg agcgaccggt ttccgccggc cgctcacttt ctcggatctt
     2941 cggcccggag gccgttgctc cgagggagtg agggaccggc ggacggggct ttgctcccac
     3001 cccggaactc gacggaaaga ccgacctcaa ggtcggcgtc ggaacgtcga tgagatgaac
     3061 gactcacggc ccgctcgcca ccggcgatc
//
LOCUS       DATDBZ010000394         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2358101, whole genome shotgun sequence.
ACCESSION   DATDBZ010000394 DATDBZ010000000
VERSION     DATDBZ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358101"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VIX62_09415"
     CDS             <1..570
                     /locus_tag="VIX62_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860489.1"
                     /translation="GAPFLFHPPGLAWLDAPVSSGYEIEGIGMSGAEYHIAAAAFAPL
                     SHHVAFDRLQLGPTVAATGAYGAVTTTAHLAALEQITEVDELKAFEATLAPPTGTALS
                     QQFLMTFLEHAAHRHSTNWFGLATPEHFWTSRPEPSYRFQEPLRELAVFRVRAIHHNG
                     DPVRERQLVLTLTMDDLGRAVVTSDGDGS"
     gene            690..1706
                     /locus_tag="VIX62_09420"
     CDS             690..1706
                     /locus_tag="VIX62_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5860490.1"
                     /translation="MNTGIIRCGIAAILFGISAPAASRLTDDMGAFTLAGLLYLGAAL
                     AVSPFVGRTPPTRAGVRASLPNLSIAVVLGGAVGPVLLAVGLGLVPAATASLLLNLEL
                     VFTTIVASLVFHEHLGRSVITGTALVVAGSVALTWSGNPDLRWGALLIAAACLCWAID
                     NSTTAAIDQISPSHITLAKGVIAGGTNLIIGLATTSTPTAGPILWALLVGGFGYGASI
                     TLWVSGARDLGAARGQLVFATAPFVGALVAWTVFNESVTRRELLAFGIAASGVAFVLG
                     SHHEHDHTHESMSHSHDHDHDEHHDHHPDALVTSGRHTHLHVHAAIAHQHPHLPDLHH
                     RHDH"
     gene            1964..>2400
                     /locus_tag="VIX62_09425"
     CDS             1964..>2400
                     /locus_tag="VIX62_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860491.1"
                     /translation="MNPTELHALFSAKEQTLLVATERARLIGLAEDDLDELLTLVRRA
                     RNKYTKLYRRQSADLVRAKSSRAGTSTSNQRTKRKAEIFEDALSRVASALAAAARATS
                     KTLKQERLAAASAAKGTPQSLEVARRRLGSPVTNPRPNVVATRA"
BASE COUNT          403 a          916 c          716 g          365 t
ORIGIN      
        1 ggcgcaccct tcctgttcca tcctccaggg ttggcctggc tcgatgctcc tgtctcctcc
       61 ggctacgaga tcgaaggaat cgggatgagt ggagccgagt accacattgc ggccgcggcc
      121 ttcgctccgc tgagccatca cgttgccttc gaccggctgc agctcgggcc gaccgtggcc
      181 gcgaccgggg cgtatggcgc agtgaccacc actgcccacc tggcggcgct cgagcagatc
      241 accgaggtcg acgagctgaa ggcgttcgag gcaaccctcg cgccgcccac cggcaccgcc
      301 ctgtcgcagc agttcctgat gacgttcctc gaacacgcgg cccaccggca ctcgaccaac
      361 tggttcgggc tggccacccc cgaacacttc tggaccagcc ggcccgagcc gagctatcgc
      421 ttccaagaac ccttgcgcga actcgcggtg ttccgtgtgc gcgccatcca ccacaacggc
      481 gatccggtcc gcgagcgcca gctcgtactc acgttgacga tggatgatct cggccgtgcc
      541 gtggtaacga gcgacggcga cggttcgtga ccgccacgct ccctgccgtc gccgcggtca
      601 tcgtggtcgt actccgccgg cgaaggtctc agtcgtaacc agcgcgacag gcaacaatgg
      661 acccgtggcc gtcgggacac cgtgacaccg tgaacaccgg gatcatccgc tgcgggatcg
      721 ccgcgatcct gttcgggatc tcggcaccag ccgcgtctcg gctcaccgac gacatgggtg
      781 ccttcaccct cgccggactc ctctacctcg gtgccgccct tgccgtcagc ccctttgtcg
      841 gtcgcacccc accgacacgc gctggggtgc gcgcctcgct ccccaacctg tcgatcgccg
      901 tcgtgctcgg aggcgccgtc ggaccggtcc tgctcgccgt cggcctcggc ctcgtccccg
      961 ctgccaccgc atcgctgttg ctcaacctcg agctcgtctt caccaccatc gtcgccagcc
     1021 tcgtcttcca cgagcacctc ggccgcagcg tcatcaccgg cacggcgctc gtcgtggccg
     1081 gcagcgtcgc actcacgtgg tccgggaacc ccgacctgcg ctggggggcg ctgctcatcg
     1141 cggctgcgtg cttgtgctgg gcgatcgaca acagcaccac cgccgcgatc gaccagatca
     1201 gccccagcca catcaccctc gccaagggcg tcatcgccgg cggcaccaac ctgatcatcg
     1261 ggctcgccac caccagcacc cccaccgctg ggccgatcct gtgggcgttg ctcgtcggcg
     1321 ggttcggcta cggcgcttcg atcaccctgt gggtgtctgg cgcacgcgac ctcggcgccg
     1381 cccgcggcca actggtcttc gccaccgcgc cgttcgtcgg ggcgctcgtc gcctggacgg
     1441 tgttcaacga atcggtcacc cgccgcgagc tactggcgtt cgggatcgcc gcgagcggcg
     1501 tcgcgttcgt actcgggagc caccacgaac acgaccacac ccacgaatcg atgtcacaca
     1561 gccacgacca cgaccacgac gaacaccacg accaccaccc tgacgccctg gtgacctcgg
     1621 gccgccacac ccacctccac gtccacgccg cgatcgccca ccaacacccg cacctccccg
     1681 acctccacca ccgccacgac cattgacctc ccgaaggcag gcgtgttgtc agcgcagcgc
     1741 agccgccgtc gatgcaggcg gcgcttcgct cgccgatcgc gcaacgccga tgcgggcgat
     1801 ccggcgttcg tggcgacccg cgagcagacg ggttcactat tcagccacac cgccatgcca
     1861 acaagagatc ggccgggctg caacgtgcgc tcaggatccg aggcacaacc gcagcccact
     1921 acagagaccg ttattgtcgt cgggacggca ccgagaggaa cgcatgaatc cgacggaact
     1981 gcacgcactg ttcagcgcga aggagcagac gctgctcgtt gctacggaac gggcgcgtct
     2041 catcggcctt gccgaggacg acctcgatga actgctcaca cttgtccgtc gcgctcgaaa
     2101 caagtacacc aaactctatc gccgccagtc agctgatctc gtcagggcga agtcgtcacg
     2161 tgcggggacg agcacgtcca atcaacgaac caagcgcaag gccgagatct tcgaggacgc
     2221 gctcagccga gttgcatcgg ctctcgccgc cgcggcgcga gcgacctcca agaccctgaa
     2281 gcaagagcgg ctcgctgcgg cgagcgcggc caagggaacc ccgcagtcgc tcgaggtcgc
     2341 caggcgacgg ctggggtcac cggtgaccaa cccgcggccg aacgtggtgg caacaagggc
//
LOCUS       DATDBZ010000395         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_754784, whole genome shotgun sequence.
ACCESSION   DATDBZ010000395 DATDBZ010000000
VERSION     DATDBZ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754784"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VIX62_09430"
     CDS             complement(<1..319)
                     /locus_tag="VIX62_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860492.1"
                     /translation="MATTVKLEAGAAGHGAEPRGKRGDDGQATYLIAIAEALWEEMER
                     DERVYMLGEDIGVYGGAFKVTEGFLDRFGAGRVIDTPIAEETIVGMAVGSAMEGLRPV
                     AEFQ"
     gene            complement(319..1389)
                     /locus_tag="VIX62_09435"
     CDS             complement(319..1389)
                     /locus_tag="VIX62_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY5860493.1"
                     /translation="MATEVDPTAPAETSGDARPLPDDELVRMLHMLKLCRYFDERMEA
                     LYRQGRLPGAIYSGRGQEGTHVGTTWALAPGDSLFPTHRDLTAQLTKGLDLKRVMAQF
                     WGRIDGYTRGRDGNSHIGDWQGSKTFTVMSHLPIAYPVAVGAALAYKLKGEPHVALAI
                     CGDGATSNGRWHEAINTSAVHRLPVVWVVNNNQFAYSTPNTQEFAVPTIAERAVAYGI
                     PGVRLDGGNVLEVFDATREAVERARAGDGPTLIESVSLRWRGHAGHDPAKYMPDEMLQ
                     EYMANRDPVRNFEEYLLTEGVVTKDDIAEIAAQVEAEFEAGYEYALASPFPEPGDATR
                     GLWVEDGYWANEPGRSGGTEAG"
     gene            complement(1401..>2036)
                     /locus_tag="VIX62_09440"
     CDS             complement(1401..>2036)
                     /locus_tag="VIX62_09440"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5860494.1"
                     /translation="KGTVSVEADICLVATGRGPVTEGLGLAEAGVATHEKGFVIVDPE
                     LRTNVDHVWAIGDVASTPLQLAHVAFTEGYAVAERIAGLEVAEIDYLNIPKVTYCTPE
                     IASVGMTEVQAREAGLDVVVDTVDFRAIGKANMLGEGGFVKLVAEVEGPVRGVHMIGP
                     HVTDLIAEGMLIVNWEALPAEVATMMHPHPSLSEGIGEAHLALAGKPLHTP"
BASE COUNT          286 a          712 c          692 g          346 t
ORIGIN      
        1 actggaactc cgccaccggg cggaggccct ccatcgcgga cccgaccgcc atgccgacga
       61 tcgtctcctc cgcgatcggc gtgtcgatca cgcggccggc gccgaaccga tcgaggaacc
      121 cctcggtcac cttgaaggcg ccgccgtaca ccccgatgtc ctcaccgagc atgtacacgc
      181 gttcatcgcg ctccatctcc tcccagagcg cctcggcgat cgcgatgagg taggtggcct
      241 ggccgtcgtc gccacgcttg ccccgcggtt ccgcgccgtg gcccgccgca cccgcttcga
      301 gcttcacggt ggtcgccatc atcctgcctc cgtgccgccg gagcggccgg gctcgttggc
      361 ccagtagccg tcctccaccc agagccctct cgtcgcgtcg cccggctccg ggaacggcga
      421 ggcaagggcg tactcgtagc cggcctcgaa ctccgcctcg acctgcgccg cgatctcggc
      481 gatgtcgtcc ttcgtgacga cgccctcggt cagcaggtac tcctcgaagt tgcgcacggg
      541 gtcgcggttg gccatgtact cctgcagcat ctcgtcgggc atgtacttcg ccgggtcgtg
      601 acccgcgtgc ccccgccagc gcagggagac cgactcgatc agggtgggcc catcgccggc
      661 gcgggcgcgc tcgaccgcct cacgggtggc atcgaacacc tccagcacgt tcccgccgtc
      721 gagtcgcaca ccggggatgc cgtaggcgac cgcgcgctcg gcgatcgtgg gcaccgcgaa
      781 ctcctgggtg ttgggcgtgg agtacgcgaa ctggttgttg ttcacgaccc agaccaccgg
      841 tagccggtgc acggccgagg tgttgatcgc ttcgtgccag cgtccgttcg acgtggcacc
      901 gtccccgcag atcgccaggg cgacgtgcgg ttcacccttc agcttgtacg cgagggcagc
      961 gcccaccgcg accggatagg cgatgggcag atgcgacatc accgtgaacg tcttgctgcc
     1021 ctgccagtca ccgatgtggc tgttgccgtc acgcccacgt gtgtagccgt cgatgcgccc
     1081 ccagaactgc gccatcacac gcttgaggtc gaggcccttc gtgagctgcg ccgtcaggtc
     1141 acggtgggtc gggaagagcg agtcacccgg cgccaatgcc caggtcgtgc cgacgtgcgt
     1201 gccttcctgg ccccggcccg aatagatcgc gccgggcagg cggccctggc ggtataaggc
     1261 ctccatgcgc tcgtcgaagt agcgacagag cttcagcatg tgcaacatgc gcacgagttc
     1321 gtcgtcgggc agcggtcgtg cgtcgcccga cgtctcggcg ggggcggtgg ggtcgacctc
     1381 ggtcgccacg cgtgggctcc ttagggggtg tggaggggtt tgccggcgag ggcgaggtgg
     1441 gcctcgccga tgccttcgga cagggatgga tgtgggtgca tcatggtggc gacctctgcg
     1501 gggagcgcct cccagttcac gatcagcatg ccttcggcga tgagatccgt cacgtgcggg
     1561 ccgatcatgt gcacgccgcg caccggtcct tcgacctcgg ctacgagctt cacgaatccg
     1621 ccctcgccca gcatgttggc cttgccgatg gcgcggaaat cgacggtgtc gacgaccacg
     1681 tcgagtccgg cctcacgggc ctggacctcg gtcatgccga cgctcgcgat ctcgggcgtg
     1741 cagtaggtga ccttcgggat gttcaggtag tcgatctcgg cgacctcgag gcccgcgata
     1801 cgttcggcga cggcgtagcc ctcggtgaac gccacgtgcg cgagctgcaa cggggtggag
     1861 gcgacgtcgc cgatcgccca gacgtgatcg acgttcgtgc ggagctcagg gtcgacgatc
     1921 acgaacccct tctcgtgggt ggcgacgccg gcttcggcca ggccgagtcc ctcggtcacg
     1981 gggccgcggc cggtcgcgac gaggcagatg tcggcctcga ccgagaccgt gccctt
//
LOCUS       DATDBZ010000396         4715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1771675, whole genome shotgun sequence.
ACCESSION   DATDBZ010000396 DATDBZ010000000
VERSION     DATDBZ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771675"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VIX62_09445"
     CDS             <1..453
                     /locus_tag="VIX62_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5860495.1"
                     /translation="IMNIGAFAATVAVSRRHPTLRIADFAGLRETAPLIAIGMAVFMI
                     SLAGVPPTGGFWGKLLIFQAAIERGGTLGSALAIVMLVNSVVSVFYYLAVPREMFLRD
                     AADRSRLRVPALVTAVVAVAMIAIVSIFVLPGAITRLAELSTLVGVGP"
     gene            545..1432
                     /locus_tag="VIX62_09450"
     CDS             545..1432
                     /locus_tag="VIX62_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEY5860496.1"
                     /translation="MTVTPGINRAKTWVLLAAMMGLFLAVGALVGGRAGMTIALVMGL
                     GFNFAMYWWSGSIAVRSTRSREVGEAEYPQLYRIVGELTQTNDMPMPGVYVSEMQQPN
                     AFATGRNPQNAKVAVTQGILQLLDERELRGVLAHELSHVANRDILVGSIAAAIGTSIS
                     YLAWMALWFGGDDEGGNPIVMLAAWLLAPIAAGLIQMAVSRSREFQADESGAFLSSDP
                     QALASALQKLEQTSRRVPPPHSLQPGEAHMFIVNPLSALKGRGLTSLFSTHPPTEERV
                     ARLMAMQDETYRRQGPIGN"
     gene            complement(1500..2882)
                     /locus_tag="VIX62_09455"
                     /pseudo
     CDS             complement(1500..2882)
                     /locus_tag="VIX62_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
     gene            complement(2964..4346)
                     /locus_tag="VIX62_09460"
     CDS             complement(2964..4346)
                     /locus_tag="VIX62_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5860497.1"
                     /translation="MDLERLKARAAEIADTETKVWLTRTTASEKLYQRATKTLPNGVV
                     SNFQAGDPYPVYLAHGKGSTVTDVDGTTYTDFHGGFGVNVVGHAHPKIVEAIKKAADD
                     AIHFAVTTETTVALAEEICSRFQCDQMRFVNSGTEATMDAIRVARAATGRDKIVKMEG
                     SYHGHHDSVLFSVVPEADVLDLRHSVDQSGTPWYTTQPTSKGVPRALWDTTIIVPFND
                     AAAVESAFADNPGEIAAVILEPVMMNIGIVVPADGYLQALKDACERHGAVLIFDEVKC
                     GGTIAYGGAAERYGVVPHLAAWAKAIGGGATTGAFGGDASIMEHVTKGAAQQGTFNGN
                     PLSSAVGLAALTQVLTRDAYDHLGKLGTMLAEGCNRAIADSGIPAHTVDLGAKGCVSY
                     RAEPLTNYRDFLETIPDIYWASYPWMVNRGIFMTPGDEEQWTVSVQHTEDDVQKYVDA
                     FGEFCQVLTS"
     gene            complement(4407..>4715)
                     /locus_tag="VIX62_09465"
     CDS             complement(4407..>4715)
                     /locus_tag="VIX62_09465"
                     /inference="COORDINATES: protein motif:HMM:NF016355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HEY5860498.1"
                     /translation="WQVLQERAVKRNIPLKALQAGPIEDAAKGTARQTVTIVTGISDE
                     KTKEISKFVRQAVPKIQTQIQGAQVRVLGKSKDDLQTAIRAVKDHEFGLALQFTNYRP
                     "
BASE COUNT          662 a         1664 c         1628 g          761 t
ORIGIN      
        1 atcatgaaca tcggcgcctt cgccgcgacg gtggcggtct cgcgccggca cccgacgctt
       61 cggatcgccg acttcgccgg gctgcgcgag acggcgccgc tgatcgcgat cggcatggct
      121 gtgttcatga tctcgctggc cggcgtgccc cccaccggcg ggttctgggg gaagctgctg
      181 atcttccagg ccgcgatcga acggggcggc accctcggat cggcgctcgc gatcgtgatg
      241 ctcgtgaact cggtcgtgag cgtcttctac tacttggccg tgccgaggga gatgttcctg
      301 cgcgacgccg ccgaccgatc gcggctgagg gttcctgccc tcgtgaccgc cgtggtcgcg
      361 gtggccatga tcgcgatcgt gtcgatcttc gtgctcccgg gcgcgatcac ccggctcgcg
      421 gagctctcca cgctcgtcgg cgtcgggccc tgatcgcggc accctcgcgc ggaacgggga
      481 accgccgggc gcgtcgtatc gaaccgccta tgggacgatc gaaagcgaac gatcacgagg
      541 agtgatgacg gtgacacctg gcatcaacag ggccaagacc tgggtcctgc tcgcggcgat
      601 gatggggctg ttcctggcgg tgggagcgct cgtgggcggg cgggccggca tgacgatcgc
      661 gctcgtgatg ggcctggggt tcaacttcgc gatgtactgg tggtcggggt cgatcgccgt
      721 ccgctcgacg cgatcgcggg aggtggggga ggctgagtac ccgcagctct accgcatcgt
      781 gggggagctg acgcagacca acgacatgcc gatgccgggt gtctacgtct cggagatgca
      841 gcagccgaac gcgttcgcga ccgggcgcaa cccgcagaac gcgaaggtcg cggtgacgca
      901 aggcatcctg cagctcctcg acgagcgtga gctgcgcggc gtgctggccc acgagctgag
      961 ccatgtggcg aaccgcgaca tcctcgtggg ctcgatcgcg gcggcgatcg gcacctcgat
     1021 cagctacctg gcgtggatgg cgctctggtt cggcggcgac gacgagggtg gaaacccgat
     1081 cgtgatgctc gcggcatggc tcctcgcccc gatcgcagcc ggcctgatcc agatggcagt
     1141 ctcgcggtcg cgggagttcc aggccgacga gtcgggcgcg ttcctgtcga gcgatcctca
     1201 ggcgctggcg agcgcgctgc agaagctcga gcagacgagc cgccgggtgc cgcctccgca
     1261 ctccctgcag ccgggcgagg cacacatgtt catcgtgaac ccgctgtcgg cgttgaaggg
     1321 ccggggcctg acgagcctgt tctccaccca cccgccgacc gaggagcggg tggcgcgtct
     1381 gatggcgatg caggacgaga cctaccgccg ccagggaccg atcgggaact agccgaaggg
     1441 tcggtcggcg agaaggagag cgtgcccacc atgaggcccc gtccgcacca cccagccggc
     1501 tacgacgtca tcgttccgag caccgcgcgc acgtggtcga tcgtggtgcg gggattcacg
     1561 aagcacagtc gtgcgagctt ctcgccccgc caggtggtgg gttgcacgaa cgcgatctga
     1621 gcctcgagca gccggcgcca ccacgcctcg tagtccgcgc cgctccatcc gagccgcctg
     1681 aacagcacga ccgacagctc cgggtccatc acgagctcga gctcgtcgcg cccccgcacc
     1741 tcctcggccg tctcgcgcgt gagggtcagc acccgctcga tcgcctcgcg atacgcgtcg
     1801 gtcccgtacg tcgcgagcga gaaccacagg ggaaggccgc gggcgcgccg tgacaggtgg
     1861 taggcgtagt cggccgggtt ccactcgctc cacgccgcat cgggttggtt caccgtgtcg
     1921 aggtacccgg cttcctgccg gaacacgccg tacgcggtcg acgggtcgcg gtacacgagc
     1981 gcgcagcagt cgtagggggc gaacagccac ttgtgcgggt cgacgatgaa cgagtcggcg
     2041 cgctcgatgc cccggtagcg ctcgcgaacc gacggcgccg cgagcccggc ccccccgtag
     2101 gccccgtcga cgtggaacca cacccctcgc gcctcgcaca cctcggccac gcccgcgagg
     2161 tcgtcgaccg tgccggcgtt ggtcgcgccc gcactcgcga ccaccgcgaa gacgctcgta
     2221 gcctagtcgg ggccgatcgc gtcgagcgtc gaggcgagcc cggcgccggt gagccgcccg
     2281 cgttcgtcgc cccccacgtc gacgacgccc acgtcgatca cgcgggcggc ggtggcgacc
     2341 gacgagtgca cgctgtcggc cacggcgatc ttccacccgg tgggcggccg tccgcccagc
     2401 ctcgacgccg caccggcacg cgccgcgacc agcgccgaga ggttccctgc ggaccctccg
     2461 ctcacgaaca ccccgccggc gacctccggg aacccggcga gatcggccag ccagcgcagc
     2521 gcctggttct ctgcccagat cgccccggcc ccgtcgatcc atcccgctgc gatcgtcgac
     2581 gaggcgccca ccacgaggtc gaacagcacc gaggccttcg tgggcgcgcc cggcacgaac
     2641 gccatcgacg atggatggtc ggtcgagatc gtggccgggg cgagcacctc gctccagacg
     2701 cgcagcgcct cctcgccgcc gaggccctcg ggcgtcaccg tctcaccggc gcgacgggcg
     2761 agttcctcac cgctggccga gccgtcgagg ggttgcgggc tcgcgatgcg gttgcgggcg
     2821 taagccacga tcgcgcgcga cagcgcctcg gtctcgtcgg tgtagcggtg catctcgtcc
     2881 atggtggtgg tctcctccgc caacacgaag ggccggcccg agggccggcc cgccgcgtcc
     2941 gatcgctccc cgccgggcgg ggactacgag gtgaggacct ggcagaactc gccgaacgcg
     3001 tcgacgtact tctgcacgtc gtcctcggtg tgctggaccg agaccgtcca ctgctcctcg
     3061 tcaccggggg tcatgaagat gccccggttg accatccacg ggtaggacgc ccagtagatg
     3121 tcggggatcg tctccaggaa gtcccggtag ttcgtgaggg gctccgcgcg gtacgagacg
     3181 cagcccttcg cgccgaggtc gaccgtgtgg gccgggatgc cgctgtcggc gatcgcccgg
     3241 ttgcaccctt cggcgagcat cgtgccgagc ttcccgaggt ggtcgtacgc atcacgcgtc
     3301 agcacctggg tgagcgccgc gaggcccacc gccgacgaca gtgggttgcc gttgaacgtg
     3361 ccctgctgcg ccgcgccctt cgtgacgtgt tccatgatgc tcgcgtcgcc gccgaaggcg
     3421 ccggtcgtcg cgccgccgcc gatcgccttg gcccacgcgg ccaggtgcgg caccacgccg
     3481 tagcgctcgg ccgcaccccc gtacgcgatc gtgccgccgc acttgacctc gtcgaagatc
     3541 agcaccgcgc cgtggcgctc acaggcgtcc ttgagcgcct gcaggtagcc gtcggccggc
     3601 accacgatgc cgatgttcat catcaccggc tcgaggatca ccgccgcgat ctcgccgggg
     3661 ttgtcggcga acgccgattc cacggctgcc gcgtcgttga acggcacgat gatcgtggtg
     3721 tcccacagcg cccgcggcac gcccttggag gtcggctgcg tcgtgtacca gggcgtgccg
     3781 ctctggtcga cgctgtgacg cagatcgagc acgtcggcct cgggcaccac ggagaagagc
     3841 accgagtcgt ggtggccgtg gtaggaaccc tccatcttga cgatcttgtc gcgcccggtc
     3901 gcggcccggg cgacccggat cgcgtccatc gtggcctcgg tgcccgagtt cacgaagcgc
     3961 atctgatcgc actggaaccg cgagcagatc tcctcggcca gcgccaccgt ggtctcggtg
     4021 gtgaccgcga agtggatcgc gtcgtcggcg gccttcttga tcgcctcgac gatcttcggg
     4081 tgggcgtgtc ccacgacgtt cacgccgaac ccgccgtgga agtcggtgta ggtcgtgccg
     4141 tcgacgtcgg tgaccgtgga tcccttgccg tgcgcgagat acacggggta tgggtcgccc
     4201 gcctggaagt tcgagaccac gccgttcggc aaggtcttcg tggctcgttg gtacagcttc
     4261 tcggacgccg tcgtccgggt cagccacacc ttcgtttccg tgtcggcgat ctccgccgcg
     4321 cgcgccttca atcgttcgag gtccacgcgt atcacctcct gagtcgggcc agggtacccg
     4381 tgggcccccg agcgcgcaat gcgaggtcag ggccggtagt tcgtgaactg cagggcgagc
     4441 ccgaactcgt ggtccttgac ggccctgatc gccgtctgca ggtcgtcctt gctcttgccg
     4501 agcacccgca cttgcgcgcc ctggatctgg gtctggatct tcggcaccgc ctggcggacg
     4561 aacttcgaga tctccttggt cttctcgtcg ctgatgcccg tgacgatcgt gaccgtctgt
     4621 cgagcggtgc ccttggcggc gtcctcgatc gggcctgcct gcagcgcctt gagcgggatg
     4681 ttccgcttca ccgcgcgttc ctgcagcacc tgcca
//
LOCUS       DATDBZ010000397         8250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2185121, whole genome shotgun sequence.
ACCESSION   DATDBZ010000397 DATDBZ010000000
VERSION     DATDBZ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185121"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VIX62_09470"
     CDS             complement(<1..894)
                     /locus_tag="VIX62_09470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5860499.1"
                     /translation="MTTIHHDVTRLLTDEMLAGFDERAATYDRENTFFSEDFEALRDS
                     GYLLATVPADMGGGGVGLDEYVKLQSRLGYYAAPTALGVNMHLYWTGVASDLRNQGDH
                     SCDFLLDAAMRGEVLAALHGEAGNDLPLLLSSSTAERVDGGWKINGHKIFGSLTPVWT
                     LGGFHAMSTDDPAGPQIVHGFVDHDAAGLVVVDTWDTFGMRATQSQDTVFHDVFVPDS
                     QVALVCPAGFAGAGMFQVAIFAWALMGFASVYQGIAHRAFDITVEKAPQRSSVALTRS
                     MAYHPEVQHLVADMRIALDAID"
     gene            complement(970..1224)
                     /locus_tag="VIX62_09475"
     CDS             complement(970..1224)
                     /locus_tag="VIX62_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEY5860500.1"
                     /translation="MSLFVIERHFAEMLDVAGLDHDGIKAVNDDVGINWLYSFLSADK
                     KKTYCLYEAPSAEAIREAAARLGIPADEVVPVEQFGPAPV"
     gene            1387..4866
                     /locus_tag="VIX62_09480"
     CDS             1387..4866
                     /locus_tag="VIX62_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5860501.1"
                     /translation="MPTTTSTILFTDAVESTSLRTELGETAADELFRDHERRLQEIVT
                     DHRGTVVKTAGDGIMAAFEASTDAVLAAIAVQQCVARRRPTMQVRVGLGVGDVSWEDG
                     DCYGLPVVVAARLQSAAAAGQIVASALVRQLAGDRSGARFRALDPLPLKGLPEPIDAF
                     EVEWSPIELSFPDGPFAVALPSALAVESSAPFVGRTGEWETLLEAWAAVGAGARQAVL
                     IGGEAGAGKTRLAVEFARWAGRRGAAVAYGACDAELAVPYQPFVLALDHLLRAMPDEL
                     LDHHREQVAEMAILLPQIERRIPGLQRAPSADPDTDRYRFFTAVDAMLGLAAAGRPVV
                     LLIDDLHWAGHQTLALLRHLTRSSSEVSLLLLVTFRDTVAEIPESLATLLAELRRTRA
                     ARRISLSGLDPSSVEDFVATMVGHDLDRDLRIVAQSMAARTGGNAFYLGELWQHLISS
                     GRVAREGGRWTVQSDAGIDAVPESVREVESARLVTLSAPARHVVELMATGGQRLPLRV
                     VTVASDLAPEALDVAVDELVDARLLVEVGGTLPRYEFVHAIVRDSVAQTVRAAARVRL
                     HHRLAVAYESVFEGDRRPVLAELARHFTAAASIAGPAKAIYYARRAATQAMRSVAYDE
                     AIAHLQSALALSPADSTEAIDLELELGSALSRKGDNQQAMQVFQRAFRAARERGLVEQ
                     AVQAAVGFDFAVQMPGLPGDESVRIMSEALELVGEEDSPLRARVQASLALALALAGRM
                     KEAKPVGDASMIAARRHGDPTVLMSTLQCALIYEENSFRYTETAAEVCELALALDDLW
                     AYCFARGCYVRGLMRLGRLVDARAALPELREAAERGQFVIYRYQSITFDVMFALIDGR
                     LDEAEELAERASAFAAAAHTEFGAGVYGVQMYAIRREQGRLEEVVPAMRVAAALQADQ
                     AVWRPGLTALFADVGMFDEARREFGTLASEGFAAVPRDATWPGSLSYLSEVCTALADR
                     EQADVLYRELDTFARQTMQVGFTVNLGPADRLRGNLAALLGRRADAERHFMAAMELAV
                     TARSPVWRARIAHDWAVALAPQKELLREADELAVQVGMEDLVRRCRRELDALRSSAAE
                     PPAVSLPDKLSTREVEVLRLVAQGRSNREIGEQLFISGNTVANHVRAVLQKTGTANRA
                     EATAYAARNGLLV"
     gene            complement(4868..5020)
                     /locus_tag="VIX62_09485"
     CDS             complement(4868..5020)
                     /locus_tag="VIX62_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860502.1"
                     /translation="MAGSAFRDITDRADIEVLVRNFYRDAAMERLLSGVSASGDAPIE
                     PVWRRS"
     gene            complement(5020..5739)
                     /gene="ric"
                     /locus_tag="VIX62_09490"
     CDS             complement(5020..5739)
                     /gene="ric"
                     /locus_tag="VIX62_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HEY5860503.1"
                     /translation="MMTIDLSTTLAEAVNACPPLAREFERRGLDYCCGGRRTIGDACT
                     SIGLDPEAVVAELSRVDLATDDRGWSTMAADVLVEHIEVTHHRYLWDELPRVTSLLAK
                     VVSVHGARHPELTEIAVCFADVRADLEPHMLKEERVLFPMIRELATSTGVPAFHCGSL
                     RNPISVMLSEHDAVGELLARLRHLTDGYTPPADGCASYVACFTAMAELEADTHLHIHK
                     ENNVLFPMVVQMEAERAAMLT"
     gene            complement(5775..6593)
                     /locus_tag="VIX62_09495"
     CDS             complement(5775..6593)
                     /locus_tag="VIX62_09495"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HEY5860504.1"
                     /translation="MTLTHDERPAGERVGERAGWRAVAMPSEHGGWGLTLEPVLLGLL
                     VAWSGAGMALGIAAFAAFLLRTPAKLVAVDVRRGRWLDRSRLALRIAIVELILLASSV
                     AVAVALAGWSWLLPVVVATPLIAVEFSYDIRSRGRRLVPELCGAVGIAAIAASIVLAD
                     GGSGGLAAGVWLVLAARAIGAIPFVRVQIMRLRRGQGPVWQSDLAQVFAIAVAAVAVI
                     VDRRLLLGFAGVAVVAAAQVRWVRRAPMPAKQLGVLELAIGLALVDVTLTGVLW"
     gene            6739..7617
                     /locus_tag="VIX62_09500"
     CDS             6739..7617
                     /locus_tag="VIX62_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5860505.1"
                     /translation="MEPTERRVHLQKLDVTVRIHEIGEGPPVVLIHGASVAGISWINL
                     VEGLEGFRCILLDRPGCGLSDPMPGAAGKADIDAVKRSGDHLLPDLLDALELPAAHVL
                     ATSFGAYFALRAAAAAPERVQRVLLYSWSMGVPMDHTPFVMRVAGIPAIGQITARMPI
                     SRSSAKMMLRQVGLRGAIDSGKFNAEMLDWFVALLRYTPTMLNEIRATPKVIQPIKGI
                     NQAMLFTDDMLARVAGPVHCFWGEDDPNGGAETARQFVARLPSCTLELVPDAGHAPWI
                     DETELAIASTRAFLSG"
     gene            7711..8133
                     /locus_tag="VIX62_09505"
     CDS             7711..8133
                     /locus_tag="VIX62_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY5860506.1"
                     /translation="MTRSDRPVVKARRALTLAAAVIVGALALTSCSDESGAAGQDATV
                     LDVTLGRFILEPSVLQAPAGDLVLRITNGDPDMVHDFVVHGKGTRRLAPGESQTLKIP
                     EVAAGEYRMWCDVPGHAAAGQSGTLVVTPAVEAAPVSS"
BASE COUNT         1308 a         2793 c         2853 g         1296 t
ORIGIN      
        1 gtcgatggcg tcgagcgcga tccgcatgtc ggccaccaga tgctgcacct ccgggtggta
       61 cgccatcgag cgggtgagcg ctaccgacga ccgctgggga gccttctcga cggtgatgtc
      121 gaacgcacgg tgggcgatgc cctgatacac cgacgcgaag cccatgagcg cccaggcgaa
      181 gatggcgacc tggaacatgc ccgcgcccgc gaacccggcg gggcacacga gcgccacctg
      241 cgagtcgggc acgaagacgt cgtggaacac ggtgtcctgg ctctgtgtcg cccgcatccc
      301 gaaggtgtcc cacgtgtcga cgaccacgag gcccgcggcg tcgtggtcga cgaacccgtg
      361 gacgatctgc ggtcccgcgg gatcgtcggt gctcatcgcg tggaacccgc cgagggtcca
      421 cacgggggtg agcgaaccga agatcttgtg gccgttgatc ttccagccac cgtccacccg
      481 ctcggcggtg ctcgaggaca gcagcaacgg gaggtcgttg ccggcctcgc cgtgcaatgc
      541 cgcgagcacc tcgccgcgca ttgccgcgtc gagcaggaag tcgcaggaat gatcgccctg
      601 gttgcgcaga tccgacgcca caccggtcca gtacaggtgc atgttcaccc cgagggcggt
      661 gggggcggcg tagtaaccga gccggctctg caacttgacg tactcgtcga gccccacccc
      721 tccaccaccc atgtcggcgg gaacggtggc gagcaggtag cccgagtcgc gcagtgcctc
      781 gaagtcttcg ctgaagaacg tgttctcccg gtcgtaggtc gcggcacgct cgtcgaaccc
      841 ggcgagcatc tcgtcggtga gcaggcgcgt gacatcgtgg tgaatggtgg tcatggtgcg
      901 tggccccttt cagggttgat gatccgtttg gggatcgttg tgaggtcggc cggtggttcc
      961 ggccgcgtgt cagaccggtg ccgggccgaa ctgctccacg gggacgacct cgtcggccgg
     1021 gatgcccagt cgtgctgcgg cctcgcgtat ggcctcggcg ctgggcgctt cgtacaggca
     1081 ataggtcttc ttcttgtccg ctgagaggaa ggagtagagc cagttgatcc cgacgtcgtc
     1141 gttgaccgcc ttgattccgt cgtggtcgag accggctacg tcgagcatct cggcgaagtg
     1201 tcgctcgatg acgaaaagac tcatggcgtc ccctttgctc ctcggatgtc gaccacggta
     1261 gagagcgtcc gccaccgtgt catgaccggt tcgggccatt tcctgtttcc ttgccgtcct
     1321 cggtgggcca caatgggata gtcagtcctg actagtgggt tcccctcgct ggggaaaggg
     1381 gcggtcgtgc cgaccaccac gtcgacgatc ctcttcaccg atgcggtgga gtcgacgtcg
     1441 ctgcgaaccg aactgggtga aacagccgcc gacgagctgt tccgcgacca cgaacggcgc
     1501 ctgcaggaga tcgtcaccga tcaccgtggc acggtggtga agacggcggg cgacggcatc
     1561 atggcggcct tcgaagcgtc gacggacgcc gtgctggcgg cgatcgccgt gcaacagtgc
     1621 gtcgcccgtc ggcgcccgac gatgcaggtc cgggtcggtt tgggggtcgg tgacgtcagc
     1681 tgggaggacg gcgactgcta cggcctgccg gttgtggtgg ccgctcgact gcagtccgcg
     1741 gcggcggccg gacagatcgt ggcgagcgcg ctcgtccgtc agcttgccgg tgaccggtcg
     1801 ggggcgcggt tccgggcact cgacccgctg ccgttgaagg gtcttcccga gccgatcgac
     1861 gcgttcgagg tcgagtggag cccgatcgag ctgtcctttc cggacggccc gttcgccgtc
     1921 gcgctgccgt cggcgttggc agtggagagc agcgcgccct tcgtcggtcg cacgggtgag
     1981 tgggagacat tgctcgaggc gtgggccgct gtcggtgccg gagcacgcca agcggtgctg
     2041 atcggtggtg aagccggcgc gggcaagacc cggctcgccg tcgagttcgc ccggtgggcc
     2101 ggccgtcggg gtgcggcggt ggcgtacggc gcgtgcgacg cggagctggc cgtcccctac
     2161 cagccgttcg tgctggcgct cgaccacctg ttgcgggcga tgcccgacga actgctcgac
     2221 caccaccgcg agcaggtcgc cgagatggcg atactcctgc cgcagatcga gcggcggatc
     2281 ccaggtctgc agcgtgcgcc cagcgccgac cccgacaccg atcggtaccg cttcttcacc
     2341 gcggtggacg cgatgctcgg gctggcggca gcaggacgtc cggtggtcct gctgatcgac
     2401 gatctgcact gggccggcca tcagacgctc gcgctcctcc ggcacctcac ccgctcgagc
     2461 agcgaggtgt cactgttgct gctggtgacc tttcgcgaca cggtcgccga gatacccgag
     2521 tcgctcgcca cgctgctcgc cgaacttcgt cgcaccaggg ccgcacggcg gatctcgttg
     2581 tcgggcctcg acccgtcgag cgtggaggac ttcgtggcga cgatggtggg acacgacctc
     2641 gaccgcgacc tgcgcatcgt cgcccaatcg atggcggcgc gcacgggcgg caacgccttc
     2701 tacctgggcg aactgtggca acacctgatc tcgtcgggcc gggtcgctcg cgagggcggc
     2761 cgctggaccg tgcagagcga cgccggcatc gatgccgtgc cggagagcgt gcgcgaggtg
     2821 gagtcggccc gcctggtcac gctgtcggcc ccggcgcgtc acgtggtgga gttgatggcg
     2881 accggaggcc agcggctgcc gctccgtgtc gtgaccgtgg cgagcgacct cgcgcccgag
     2941 gcgctcgatg tcgccgtcga cgaactggtc gacgcgaggt tgctcgtcga ggtcggaggg
     3001 acgttgccga ggtacgagtt cgtccacgcc atcgtgcgcg acagtgtcgc ccagacggtg
     3061 cgcgcggcgg cacgagttcg cctgcaccac cggctggcag tcgcctacga gtcggtgttc
     3121 gagggcgacc ggcgaccggt gctcgccgaa ctggcgcgtc acttcaccgc ggcagcctcg
     3181 atcgcggggc cggcgaaggc catctactac gcccggcgtg ccgcgacgca agcaatgcga
     3241 tcggtcgcct acgacgaagc gatcgcccat ctccaatcgg ctctggcgct cagcccggcc
     3301 gactcgaccg aggcgatcga tctcgaactc gaactcgggt cggcgctgtc ccgcaagggc
     3361 gacaaccagc aggcgatgca ggtcttccag cgggccttcc gggcggcgcg cgaacggggg
     3421 ctggtcgagc aggcggtgca ggctgccgtg ggtttcgact tcgccgtgca gatgcccggg
     3481 ttgcccggtg acgagtcggt acgcatcatg tcggaggcgc tcgaactggt gggtgaggag
     3541 gacagcccgc tgcgcgcccg ggtccaggca tcgctggcgc tggcgctcgc gctcgccggg
     3601 cggatgaagg aggccaagcc ggtgggcgat gcgagcatga tcgcggcgcg ccgccacggc
     3661 gacccgacgg tcctcatgtc caccctgcag tgcgcattga tctacgagga gaactccttc
     3721 cgctacacgg agactgccgc cgaggtgtgc gaactggcgc tggcgctcga cgacctctgg
     3781 gcgtactgct tcgcccgcgg gtgttatgtg cgtggcctga tgcgactggg acggttggtc
     3841 gacgctcgag cggcgctgcc ggagctgcgc gaggccgccg agcggggcca gttcgtgatc
     3901 taccgctacc agtcgatcac gttcgacgtg atgttcgccc tgatcgacgg acgcctcgat
     3961 gaggcggagg aactggccga gcgggcgtcg gcgttcgcgg cggcggcgca caccgagttc
     4021 ggtgccggcg tgtacggcgt gcagatgtac gccatccggc gcgagcaggg ccggctggag
     4081 gaagtggtgc cggcgatgcg ggtcgccgcc gcgctgcagg ccgaccaggc ggtgtggcga
     4141 ccgggtctca ccgcgctctt cgccgacgtc ggcatgttcg acgaggcccg gcgggagttc
     4201 ggaacgctcg cctccgaggg ttttgcggcg gtgccgcgcg acgccacctg gccggggagc
     4261 ctcagctacc tctccgaggt gtgcacggcg ctcgccgacc gcgagcaagc ggacgtgctg
     4321 taccgcgagc tcgacacgtt cgcgcgccag acgatgcagg tggggttcac ggtcaacctc
     4381 ggacctgcag accgcttgcg cggcaatctg gctgcgctgc tcgggcgacg cgccgatgcg
     4441 gagcgccact tcatggcagc gatggaactc gccgtcaccg cccgctcacc ggtgtggcgg
     4501 gcccggatcg cgcacgactg ggcggttgcc ctggcgccgc agaaggaatt actgcgcgag
     4561 gccgacgagc tcgcggtgca ggtcggcatg gaggatctgg tgcgccgctg ccggcgcgag
     4621 ctcgatgcgc tgcggagttc ggccgccgag ccaccggcgg tgtcgttgcc cgacaagttg
     4681 tcgacgcgtg aggtggaggt gctgcgtctc gttgcgcagg gccgctccaa tcgcgagatc
     4741 ggcgagcagc tgttcatcag tggcaacacg gtggccaacc acgtgcgtgc cgtgctgcag
     4801 aagaccggta ccgcgaaccg cgccgaggcc accgcctacg cggccaggaa cggcctgctg
     4861 gtgtgagtca ggagcgccgc cacaccggct cgatcggtgc gtcaccactc gcggacacac
     4921 cggacagcag gcgctccatc gccgcatccc ggtagaagtt gcgcaccagc acctcgatgt
     4981 cggcgcggtc ggtgatgtcc cggaacgcgc tccccgccat cacgtcagca tcgccgcacg
     5041 ttcggcttcc atctgcacga ccatggggaa cagcacgttg ttctccttgt ggatgtgcag
     5101 gtgggtgtcg gcctccagct ccgccatcgc cgtgaagcac gcgacgtacg aggcgcaacc
     5161 gtccgccggt ggcgtgtagc cgtcggtcag gtggcgcagc cgggcaagca gctcgccgac
     5221 cgcatcgtgc tcgctcaaca tcaccgagat cggattgcgc aacgatccac agtggaacgc
     5281 cggaactccg gtcgacgtgg cgagctcgcg gatcatcggg aagagcacgc gctcctcctt
     5341 cagcatgtgc ggttccaggt cagctcgcac atcggcgaag cacaccgcga tctcggtcag
     5401 ctccgggtgt cgcgcgccgt gcaccgacac gaccttggcc aacagcgacg tcacccgcgg
     5461 caactcgtcc cacaggtacc gatgatgggt cacctcgatg tgttcgacca ggacgtcggc
     5521 ggccatcgtc gaccaccccc gatcatcggt cgcgagatcc actcgggaca gctcggcgac
     5581 cacggcttcg gggtccaacc cgatcgaggt acaggcgtcg ccgatcgtac gccggccccc
     5641 gcagcagtaa tcgagaccac gccgctcgaa ctcccgggcc agcggcggac acgcgttcac
     5701 cgcctcggcc aacgtcgtcg acaagtcgat cgtcatcatt tgtctctcct gctccacgaa
     5761 tccatccgat ccgttcacca cagcactccc gtcagagtga cgtcgacgag cgcgaggccg
     5821 atggccagct ccaacacacc gagctgtttc gccggcatgg gcgcacgacg gacccacctg
     5881 acctgcgccg cggccaccac ggccacccct gcaaaaccga gcagcagccg tcggtcgacg
     5941 atcaccgcca ctgcagcgac cgcgattgcg aacacctgtg ccagatcgct ctgccacacc
     6001 ggaccctgcc cgcgacgaag acgcatgatc tgcacccgca cgaacgggat cgcaccgatc
     6061 gcccgagccg ccaacacgag ccacacacct gcggcgagtc cgccgctgcc tccatcagcg
     6121 agcacgatcg acgccgcgat cgcagcgatg ccgacggcac cgcacaactc gggcaccagg
     6181 cggcggccgc ggctgcggat gtcgtacgag aactccactg cgatcagggg cgttgcaacc
     6241 accaccggca agagccacga ccaacccgcc agggcgacgg cgaccgcgac actcgacgcg
     6301 agcaggatca gctcgacgat cgcgatccgc agtgccaggc gggaccgatc gagccagcgt
     6361 ccgcgccgca catcgaccgc gaccaatttg gccggcgtcc gaagcaggaa cgcggcgaag
     6421 gcggcgatgc cgagcgccat gcccgcaccg gaccacgcga cgagcaggcc gagcaacacg
     6481 ggctcgaggg tcaatcccca gcctccgtgc tcgctgggca tcgccaccgc acgccaaccg
     6541 gcgcgctcgc cgacgcgctc gccggccggg cgctcgtcgt gcgtcagagt cacacttgca
     6601 gactcgtgga ccggacgggc gaacaccagg gaccaaagac cccttcgagg cccgagagga
     6661 cgagtgtcat actgaggaat ggggtcggac gaggccaggt atcggcaagt cgagcgacgg
     6721 ttgtgggagt cgttcgggtt ggaaccgacc gaacggcggg tccatctgca gaaactggac
     6781 gtcaccgtgc ggatccacga gatcggggag ggacctccgg tcgtcctgat ccacggggcg
     6841 tcggtggccg gcatcagctg gatcaacttg gtcgagggcc tcgaagggtt ccgctgcatc
     6901 ctgctcgacc gacccggctg tgggctcagc gacccgatgc ccggcgccgc aggaaaggcc
     6961 gacatcgacg ccgtgaagcg atcaggcgac cacctgctcc cggacctgct cgatgccctc
     7021 gaactgcccg cggcccatgt gctggccacg tccttcggcg cgtacttcgc cctccgggcc
     7081 gcggccgccg ctcccgagcg cgtccagcgt gtgctgttgt acagctggtc gatgggcgtt
     7141 ccgatggacc acaccccatt cgtcatgcgg gttgccggca ttccggccat cggtcagatc
     7201 accgcgcgga tgcccatcag ccgctccagc gccaagatga tgcttcgcca ggtgggtttg
     7261 aggggcgcca tcgactcggg caagttcaat gccgagatgc tcgactggtt cgtcgcgctg
     7321 ctgcggtaca ccccgacgat gctcaacgag atccgggcga cgcccaaggt gatccaaccg
     7381 atcaagggca tcaaccaggc gatgttgttc accgacgaca tgctcgcccg ggtcgccggt
     7441 ccggtgcact gcttctgggg cgaggacgac ccaaacggcg gagcggagac cgcgcggcag
     7501 ttcgtggccc ggctcccgag ctgcaccctc gaactcgtac cggatgccgg ccatgccccc
     7561 tggatcgacg agaccgaatt ggccatcgcg tcgacgcggg cgttcctgag cggctgaaca
     7621 tcgacgctgg aggagcgcac gtcgagtcgt cctccaggtt gtgaccttcg gccccagcga
     7681 cgccggtgag tcatgccgac aatggtcggc atgactcgtt ccgatcggcc agtcgtcaag
     7741 gcacggcgcg cgctcacgct cgccgccgcc gtcatcgtcg gcgcactggc gctgacgtcg
     7801 tgttccgacg aatccggcgc ggccgggcag gacgcaaccg tcctcgacgt gacgctcggg
     7861 cggttcatcc tcgagccctc cgtgttgcag gcgcccgctg gcgatctcgt cctgcggatc
     7921 acgaacggag atcccgacat ggtgcacgac ttcgtcgtcc atgggaaggg aacgcgtcgt
     7981 ctcgcgccgg gcgagtcgca gacattgaag atccccgagg ttgctgccgg tgagtaccgg
     8041 atgtggtgcg acgtgcccgg gcacgccgct gccggtcaga gcggaaccct ggtcgtcacc
     8101 ccggccgtgg aggcagcccc agtgtcgtca taggttcccg acgactcgac gaagatgagc
     8161 cattcgtggg atcgaaagcg atcgactctc gcgtgttgtg gtgcccccgg agagattcga
     8221 actcccgcac atggttccgg aaaccaatgc
//
LOCUS       DATDBZ010000398        12162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1569039, whole genome shotgun sequence.
ACCESSION   DATDBZ010000398 DATDBZ010000000
VERSION     DATDBZ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12162
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569039"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VIX62_09510"
     CDS             <1..1381
                     /locus_tag="VIX62_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5860507.1"
                     /translation="AARTRADEDVAFLAYHDELTGLPSRSMFEELLTLSIDRASRHDG
                     AVAAVCVDVDDFRLVNDSLGHQVGDQLLRMVADRLREATRETDLVARRGGDQFLMLIA
                     DLERESAGDMDAAVVRAEAAAQRVQEAMAAPFMVGGTELYLSVSMGISLYPLDGEDAN
                     TLHRNAEAAMYESKKSGSSGYVVSSRGAFDSAAKLQFVTRLRKAVESQRWALHYQPIL
                     ELDTGRMKGVEALIRWIEPDGTMVPPNQFIPLAEELGLIELIGDWVVRELVYQAQAWR
                     ELDIDLEIGFNLSPRQFWQPDLSDRILTRILDGGIEPAKVMVEITETSAMMDPDRAQE
                     ILWQLHSGGLQIAIDDFGTGYSSLSRLREMPVSVLKIDRSFVTHVESDPQSASIVTAF
                     LELARGLEMTTLAEGIERVEEFAFLKERGCQLGQGFLFSKPVPPEEIIALAFGGVPLP
                     KPVGADAAR"
     gene            complement(1391..3235)
                     /locus_tag="VIX62_09515"
     CDS             complement(1391..3235)
                     /locus_tag="VIX62_09515"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY5860508.1"
                     /translation="MDDRIQLWNDLAAQIAVDSIRCTTRAGSGHPTSSMSSAHLLSVL
                     YADHLRYDVGDPKSPANDRFIMSKGHAAPALYSVLKAIGAIDDEQLMTLREFGSPIQG
                     HPAPVAELPWIDVASGSLGQGLAVGLGMSLSMRLDGLPGRTWVLMGDSEIAEGSVWEA
                     MEAASFHQVDNLIGILDLNRLGQRGPTMHEWHADLFAQRAQAFGWHSIEIDGHDVTSI
                     DAAYTEAGAADKPTLIIARTEKGHGVSFLANQEGWHGKAVPADKEGAAIEELGGIRSI
                     TITPIGPEDLKPLVLGELHAAPAPVYTEPIPTRKAFGEALAWLAGHRPDVVVLDGEVG
                     NSTHTEDFEAVAPERFFQMYIAEQCVVGAQTGMQALGKTAFAATFGAFLSRAADFVRM
                     GAISRANLRLCGSHAGVSIGEDGPSQMAVEDLSLMRALHSSTVLYPADGNATIRLVTT
                     MCDLEGISYLRTTREATPVLYSADEEFPVGGSKTLRGTGDDVATLVGAGVTVHECLAA
                     ADLLAADGIAVRVLDVYSVKPIDAAAVRRALDETNLIVVAEDHRTEGGIGDAVLDALA
                     GSGPMNGSVVKLAVTDMPGSGSAQQMRAWAGIDAASIAAAVRDGVAGR"
     gene            3381..4586
                     /locus_tag="VIX62_09520"
     CDS             3381..4586
                     /locus_tag="VIX62_09520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5860509.1"
                     /translation="MTTHDPSFEALGVSDAVIETLAARGIHAPFPIQQMVIEDATEGR
                     DTLAKSKTGSGKTLGFAIPIVEQLDPDATSRPLALVLVPTRELATQVKEDFTDIAKAK
                     HLRVKAVYGGTNVKEQAKGVEQAHILIATPGRLDDLVERRMVFLKHVQIFVLDEADRM
                     LDMGFQPQVDRIVRHLPKDRQTMFFSATLDGAVGRIAEVYTRNPVRHEIESDSQTVDE
                     ADHRFVAVDRTAKLQTLIDLAHEEEQTGLTLVFVNTKRHVETLARQLRNEGVPTVTMH
                     GDMTQQARERALQRFEDKHVGVLIATDVAARGLDLDDITLVVNYDPPQDDKGYVHRVG
                     RTARAGRAGVSVTFVTPDQLGDVSRMAARLDLHEEFEQDGMKVAPPRTVFSGSSRGRR
                     SGLRARKKR"
     gene            complement(4602..6842)
                     /locus_tag="VIX62_09525"
     CDS             complement(4602..6842)
                     /locus_tag="VIX62_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5860510.1"
                     /translation="MNQDDLEAVGPEALTALSGFFPPSGSSDDTPFGAAERLRDLELR
                     YRALVDQLPAVIYIDGLGTGSQMVDVSPSIETTLGIPREEWLARAGVWEDAIHPDDRE
                     RVVEVSRHAAEFGEPFRIEYRAFHRDGRMLWIREDCVLIRDEVGEPVYWLGLMLDVSE
                     FALAQRDLQEVQTKFGALVEQIPAIVYIDEADERMTTTYVSPQIEPLLGYTQHDYMTD
                     PDLWTSMLHPDDRDGAVETYLRGRESGEPFVFEYRLVAKDGSIVWFRDSAIVLPDGEG
                     RPQFIQGVMLDITERKAAEAQIAYLAYHDKLTGLSNRAMFDELLELTMARARRNDLGV
                     AVVSVDLDDFKLVNDSLGHDIGDQLIVLLADRLREATRDTDLVARPGGDEFLLLLGDL
                     DLTPPVPGGTDGATIAAESVAVRVQEALKAPFEIAGTELYVTASQGISVFPLDAKDPA
                     GLLSNADTAMFISKKSGPGGYLVHSDRDADAMSRLSMSTRLRKAVELKQWTLHYQPLI
                     ELDSGDMYGVEALIRWPEPNGGLVPPGEFIPLAEEMGLIEAIGDWVVEEICRQDAQWR
                     AEGLTLEIGFNLSPRQLWQPELVDKIVSPIVVAGMDPTRVTVEITESTAMTDPDRTVE
                     LLTAIHDRGLKLAIDDFGTGYSSLARLKHMPVDILKIDRSFIRDVDKDRESASMVSAM
                     ISLAENLGMTPLAEGIETEGEWRFLASRHCTYGQGYFFSRPVPAPEILAMHRRSGLQV
                     LDGGAG"
     gene            complement(6984..7859)
                     /locus_tag="VIX62_09530"
     CDS             complement(6984..7859)
                     /locus_tag="VIX62_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860511.1"
                     /translation="MTRTSIVAAAALLALVGAACADTADTGAGSRSTTPPVDQDGTTL
                     VLRVTTEGGFTPVDYQLTNMPAFSLYGDGTIVTTGPMIEIYPPPALPSLQSQTVDSAG
                     VDAIVAAAFDAGLDSAGDLTDMGSVAIADAPDTVFTLHADGVDTTVRVYALMALQDRP
                     PTMSKEEWEARVALNGLVEDLMDLPSWLPDGSVQGEPVPYAAAGARVYVADYQGEQDL
                     AQSPVAWPLEPSLASFGRDDAVSGYRCGTVTGEDWTQTLEPLAEGANQLTPWTSDGRR
                     WSLRFRPLLPDERGC"
     gene            7952..9019
                     /gene="tdh"
                     /locus_tag="VIX62_09535"
     CDS             7952..9019
                     /gene="tdh"
                     /locus_tag="VIX62_09535"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010477907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY5860512.1"
                     /translation="MAATMRALRKTAREPGADLVEIAVPEPREGEVLVKVHGASICGT
                     DLHIYDWNEWADKRIPQVPMTFGHEVAGTVVAVGDEVHHLREGVFIAAETHIACGHCP
                     TCQTGRAHICENLKILGVDMDGVFAEYVVVPAQNAWIVGEGIDPDVASIMEPFGNAVH
                     ASFGTGGGEDIATNAVAVIGCGPIGMFAVAIARSLGAWKVIAIEPNEFRRAKAAEMGA
                     DVVLDPMETDPVQAVMDLTRGSGAEVVLEMSGNARAIDQGTRMLARGGRMSLLGLPDG
                     SVTLDLNDQVIFKEARLHGITGREMFRTWQQTTTLLSTGRVDVASVITHRFPLERFEE
                     AFATTASGSAGKVIMFPGGEG"
     gene            9016..10200
                     /locus_tag="VIX62_09540"
     CDS             9016..10200
                     /locus_tag="VIX62_09540"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY5860513.1"
                     /translation="MTDDALGYLKDELSTLERDGLLLHPRTLEGPQGARARYDGREVI
                     NLASNNYLGLANHPRLNAAASKAAAELGAGSGAVRTIAGQMTEHLELERRFAAFKHSE
                     AALMFQSGFTSNSGTVAAILAKEDVIVSDRLNHASIIDGARLSRAEIKVFDHADPDHA
                     DRLLTESAAPGRRQLLITDGVFSMDGDIALLPDLVEVAERHGAIMMVDDAHASGVLGT
                     GGSGTVDHFGLHGRVDVQVGTLSKAIGVIGGFIAGPAHLIEWLQNRGRPYLFSTSAPP
                     AVTAACIAALDVLRDEPERLERLWANTATLKEGLALLGFDTGTSQTPITPVITGEADV
                     TQRFADRLFEEGVFCPAIVFPTVAKGSARVRTIVTADHTDADLTEALEIFGRVGRELG
                     LV"
     gene            complement(10197..10865)
                     /locus_tag="VIX62_09545"
     CDS             complement(10197..10865)
                     /locus_tag="VIX62_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5860514.1"
                     /translation="MVTKTDPNAKPRERLTRERVIDAALRVMDSEGLDAVSMRRVARE
                     VGVEAMSLYNHVRDKEDLLAGIQQRVFSEFAFDADPGDPYGNGVRVAHAWRDLMRTHP
                     ALIEIMAEKHQAPISIEALRPMEKALEVLRSMGVPDPDVMQVFHAFGGYIQGFVMMER
                     QLAFAERDREHFDDVLDQLPSHDLPCLAAAMPYVADCDLDQQFDFGLDLMLQGLKSRF
                     AQDR"
     gene            10983..>12162
                     /locus_tag="VIX62_09550"
     CDS             10983..>12162
                     /locus_tag="VIX62_09550"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5860515.1"
                     /translation="MKLNPETLAKASSHHPWRTVIVWVLVLFAAGASAATLLGPALTT
                     DFDFTNSPEAKQADQILQERRLTQDTITETFIVTGEPGAVEDPDFVAGVNTFLTDLNG
                     LGTDVFQALPAAFPLTEEQAADPQVAALGPIPSADGSAVLFTGIYAGDLDEATPHFED
                     VDAIREQVSGLEGIDEAHMLGQVSSSEDFKVISEEDLQFGESIGIVAAIVVLLIVFGA
                     IVAGYLPLLLTVFFTLPITLGIVGLFGTLWDFSFFTPNLISMMGIAVGVDYALFIVSR
                     YREERHNGRKKNDAIRASGATASRAVFFSGLTVVLALAGMLLVPTTIFRSLAGGAIIV
                     VLVSVAVSMTLLPALLSVLGEWVNWPWLTRGRTLLVWLAMVVAAGVVGGGLGAVGAPP
                     A"
BASE COUNT         1905 a         4040 c         4239 g         1978 t
ORIGIN      
        1 cgccgcgcgc acgcgcgccg acgaggacgt cgcgttcctc gcctaccacg acgaactcac
       61 cggcctgccg agccgctcga tgttcgagga gctgctgacc ttgtcgatcg accgggcgag
      121 ccggcacgac ggtgcggtcg cggcggtgtg cgtcgacgtc gacgacttcc ggttggtcaa
      181 cgattcgctg ggccatcagg tgggcgacca gctcctgcgg atggtggccg accgcttgcg
      241 cgaagccacc cgtgagaccg acctggtcgc acggcgcggc ggcgaccagt tcctgatgtt
      301 gatcgcggac ctcgagcggg aatcggccgg tgacatggac gccgccgtgg tgcgagccga
      361 ggctgccgcc cagcgcgtcc aggaggcgat ggccgcgccg ttcatggtgg ggggcaccga
      421 gctctacctg tcggtgagca tgggcatcag cctctatcca ctcgacggcg aggatgccaa
      481 cacgctccac cgcaacgccg aggccgcgat gtatgaatcg aagaagtctg ggtcctcggg
      541 ctacgtggtc tcttcccgcg gggcgttcga ttccgccgcc aagctccagt tcgtgacccg
      601 ccttcgcaaa gcggtggaga gccaacggtg ggcgctgcac taccaaccga tcctggagtt
      661 ggacaccggc cgcatgaagg gcgtggaagc gctgatccgt tggatcgagc ccgacggcac
      721 gatggtgccc cccaaccagt tcatcccgct cgccgaggag ctggggctga tcgagctgat
      781 cggtgactgg gtggtgcgcg aactcgtgta tcaggcgcag gcgtggcgcg agctcgacat
      841 cgacctggag atcggtttca acctctcgcc tcggcagttc tggcagcccg acctgtccga
      901 ccggatcctg accaggatcc tggacggcgg gatcgaaccc gccaaggtga tggtcgagat
      961 caccgagaca tccgcgatga tggaccccga ccgtgcacag gagatcctgt ggcagctcca
     1021 cagcggcggt ctgcagatcg cgatcgacga cttcggcacg gggtactcct cgttgtcccg
     1081 cctacgcgag atgccggtga gcgtgctgaa gatcgaccgc tcgttcgtca cccacgtcga
     1141 gtccgatccg cagtcggcga gcatcgtgac ggcgttcctc gagctggccc gcggactgga
     1201 gatgaccacc cttgccgagg gcatcgagcg ggtggaagag ttcgccttct tgaaggaacg
     1261 cggctgccag ctggggcagg gcttcctgtt cagcaagccg gtgccgccgg aggagatcat
     1321 cgccctcgcc ttcggcggcg tgccgctccc caagcccgtc ggcgccgacg cggctcggta
     1381 gggccgacgc tcagcgcccg gcgaccccgt ctcgcaccgc cgcggcgatc gacgccgcgt
     1441 cgatccccgc ccatgcccgc atctgctgtg cgctgcccga gcccggcatg tcggtgaccg
     1501 cgagcttgac gacggaacca ttcatcggcc cggagccggc gagcgcgtcg aggacggcgt
     1561 ccccgatgcc cccttcggtc cggtggtcct cggcgacgac gatcaggttc gtctcgtcga
     1621 gggcccggcg cacggcagcg gcgtcgatcg gcttgacgct gtagacgtcg agcacgcgaa
     1681 cggcgatgcc gtcggcggcc agcaggtcgg cggcggcgag gcattcgtgc acggtgacgc
     1741 cggcacccac gagggtggcg acatcgtcgc cggttccgcg cagggtcttc gatcccccca
     1801 cggggaactc ctcgtcggcg ctgtacagca ccggtgtggc ctcgcgcgtg gtgcgcaggt
     1861 aggagatgcc ctccagatcg cacatcgtgg tgacgagtct gatcgtggca ttgccgtcgg
     1921 cgggatagag caccgtcgag gagtgcagcg ctcgcatcag cgagaggtcc tcgaccgcca
     1981 tctgcgacgg gccgtcttcc ccgatcgaga cgccggcgtg cgagccgcac agacggaggt
     2041 tggcgcggct gatcgctccc atacgcacga agtcggcggc ccgtgacagg aacgcgccga
     2101 acgtggcggc gaacgccgtc ttgccgagcg cctgcatgcc ggtctgggcg ccaacgacgc
     2161 actgttcggc gatgtacatc tggaagaacc gctcgggcgc gaccgcctcg aagtcctcgg
     2221 tgtgcgtgga gttgccgacc tcgccgtcga gcacgaccac gtccgggcga tggccggcga
     2281 gccacgccaa cgcttccccg aacgccttcc tggtggggat gggctcggtg tagacgggcg
     2341 caggcgcggc gtggagctcg cccagcacca gcggcttgag atcttcggga ccgatgggcg
     2401 tgatcgtgat cgagcggatg ccacccagtt cctcgatcgc ggcaccttcc ttgtcggcgg
     2461 gcaccgcctt gccgtgccac ccctcctggt tggcgaggaa cgagacgccg tggcccttct
     2521 cggtccgcgc gatgatcagc gtgggcttgt cggcggcacc ggcctccgtg tacgccgcat
     2581 cgatcgaggt gacgtcgtgg ccgtcgatct cgatcgagtg ccaaccgaag gcctgcgcac
     2641 gctgggcgaa caggtcggca tgccactcgt gcatcgtggg gccgcgctgg ccgagccggt
     2701 tgaggtccag gatgccgatg aggttgtcca cctgatggaa ggaggcggct tccatggcct
     2761 cccagaccga accctcggcg atctcggagt cacccatcaa cacccatgtg cggcccggca
     2821 ggccgtcgag cctcatggac agcgacatgc ccaggcccac cgcgagccct tggcccagcg
     2881 agccgctcgc cacatcgatc caggggagct cggccaccgg ggcggggtga ccctggatcg
     2941 gcgacccgaa ctcgcgcagc gtcatcagct gctcgtcgtc gatcgcgccg atcgccttca
     3001 acaccgagta caaggcggga gcggcgtgac ccttcgacat gatgaaccgg tcgttggccg
     3061 gcgacttggg atcgccgacg tcgtagcgga ggtggtcggc gtacagcacg gagagcaggt
     3121 gggcgctcga catggacgag gtgggatgcc cggaacccgc gcgggtggtg cagcggatgc
     3181 tgtccaccgc gatctgcgcc gccaggtcgt tccacagctg gattcgatcg tccatgggtc
     3241 ccctcgtcgg tcgtggtcgg gccggttcgg caaagcgtac cgccctcagg tctgacccgc
     3301 ggtcccgtcg tcgccggccg ccccacgtcg agccttcgac ggggccttcg ggcgctgaga
     3361 acgggtacgc tcgatggcgt atgaccaccc acgatccgtc cttcgaagcc ctcggcgtgt
     3421 ccgacgccgt catcgagacc ctcgcggcgc ggggcatcca cgcaccgttc ccgatccagc
     3481 agatggtgat cgaggatgcc accgaaggcc gcgacaccct cgccaagtcc aagaccggct
     3541 cgggcaagac cctggggttc gcgatcccga tcgtggagca gctcgacccc gacgcgacgt
     3601 cgcgtccgct ggcgctggtg ctggtgccca cgcgcgagct cgccacccag gtgaaggagg
     3661 acttcaccga catcgcgaag gccaagcacc tccgcgtgaa ggcggtctac ggcggcacca
     3721 acgtgaagga gcaggccaaa ggcgtcgagc aggcccacat cctgatcgcc acgccgggcc
     3781 ggctggacga cctggtggaa cggcggatgg tgttcctcaa gcacgtgcag atcttcgtgc
     3841 tcgacgaagc cgaccgcatg ctcgacatgg gcttccagcc ccaggtggac cggatcgttc
     3901 gccatctgcc gaaagatcgg cagacgatgt tcttctccgc gacgctcgat ggcgcggtgg
     3961 gccggatcgc cgaggtctac acccgcaacc cggttcgtca cgagatcgag tccgactccc
     4021 agacggtcga tgaggccgat caccgcttcg tcgcggtcga tcggaccgcc aagctgcaga
     4081 ccctgatcga cctcgcgcac gaggaggagc agaccggtct cacgttggtg ttcgtcaaca
     4141 cgaagcgtca tgtggagacg ctggctcgcc aactgcggaa cgagggcgtt cccacggtga
     4201 cgatgcacgg cgacatgacc cagcaggcac gcgaacgcgc gctccagcga ttcgaggaca
     4261 aacacgtggg cgtgttgatc gccaccgacg tcgcggcacg tggcctggac ctcgacgaca
     4321 tcacgctggt cgtgaactac gacccgcccc aggacgacaa gggctacgtg caccgcgtgg
     4381 gccgtacggc gcgcgccggc agggcgggcg tgagcgtcac cttcgtcacg cccgatcagc
     4441 tcggcgacgt gagccgcatg gccgcccgcc tcgatctgca cgaagagttc gagcaggacg
     4501 gcatgaaggt ggcaccgccg aggaccgtgt tcagcgggag ctcacggggt cgccgcagcg
     4561 ggttgcgggc gcggaagaag cgttagcgcc ggccggccgg ctcagcccgc gccgccgtcc
     4621 agcacctgga gacccgagcg acggtgcatc gccaggatct cgggcgccgg aacgggccgg
     4681 gagaagaagt agccctgacc gtaggtgcag tggcgagaag ccaggaagcg ccattcgccc
     4741 tcggtctcga tgccttcggc gagcggggtc atgcccaggt tctcggccag ggagatcatc
     4801 gcgctcacca tgctcgccga ttcgcggtcc ttgtcgacgt cgcggatgaa cgaccgatcg
     4861 atcttcagga tgtcgacggg catgtgcttg agtctcgcca gcgaggagta gcccgtgccg
     4921 aagtcgtcga tcgcgagctt gagcccccgg tcgtggatcg ccgtgagcag ctccaccgtg
     4981 cggtcggggt cggtcatggc cgtcgactcg gtgatctcca ccgtgacgcg cgtcgggtcc
     5041 atgccggcca cgacgatggg cgagacgatc ttgtcgacca gctcgggctg ccacagctgg
     5101 cgcggtgaca ggttgaaccc gatctccagc gtcaggccct cggctcgcca ttgcgcgtcc
     5161 tggcggcaga tctcctcgac cacccagtcg ccgatcgctt cgatcagccc catctcctcg
     5221 gccagtggga tgaactcgcc cggcggcacg agtccgccgt tgggctcggg ccagcggatc
     5281 aacgcctcga cgccgtacat gtcgccgctg tcgagttcga tcaacggctg atagtgcagc
     5341 gtccattgct tgagctcgac cgccttgcgg agcctggtgg acatcgacag ccggctcatc
     5401 gcgtcggcgt cgcgatcgga gtgcaccaga tagccgcccg gacccgactt cttgctgatg
     5461 aacatggcgg tgtcggcgtt gctgagcaag ccggcgggat ccttcgcgtc gagcgggaac
     5521 acgctgatcc cctggctcgc ggtgacgtag agctcggtgc ccgcgatctc gaacggcgcc
     5581 ttgagcgctt cctgcacgcg gaccgcgacg gattccgcgg cgatcgtggc gccgtcggtg
     5641 ccgccgggca ccggaggggt gaggtcgagg tcgcccagca gcagcaggaa ctcgtcgcct
     5701 ccgggccggg ccaccaggtc ggtgtcgcgc gtcgcttctc ggaggcggtc ggccaggagc
     5761 acgatgagct ggtcgccgat gtcgtggccg agcgaatcgt tcaccagctt gaagtcgtcg
     5821 aggtcgacgg acaccaccgc cacgccgagg tcgttgcgcc gggcgcgcgc catcgtgagc
     5881 tcgagcagct cgtcgaacat cgcccggttc gacaggccgg tgagcttgtc gtggtaggcg
     5941 aggtaggcga tctgtgcctc ggcggccttg cgttcggtga tgtccaacat cacgccctgg
     6001 atgaactggg ggcggccctc gccgtccggc agcacgatcg ccgagtcgcg gaaccagacg
     6061 atgctcccgt ccttggcgac caggcggtac tcgaacacga acggttctcc ggactcgcgt
     6121 ccgcgcaggt aggtctcgac ggcgccgtcc cggtcgtcgg gatgcagcat cgaggtccag
     6181 aggtcgggat cggtcatgta gtcgtgctgg gtgtagccca gcaacggttc gatctgcggg
     6241 ctcacgtagg tggtggtcat gcgctcgtcg gcttcgtcga tgtagacgat cgccgggatc
     6301 tgttcgacga gcgccccgaa cttcgtctgc acctcctgca ggtcgcgctg agccagcgcg
     6361 aactccgaga cgtcgagcat caggccgagc cagtagacgg gttcgccgac ctcgtcgcgg
     6421 atcaggacgc agtcctcgcg gatccacagc atccggccgt cccggtggaa ggcgcggtat
     6481 tcgatccgga agggctcgcc gaactccgcg gcgtgcctgc tgacctcgac gacccgctca
     6541 cgatcgtccg ggtggatcgc gtcctcccac acaccggcac gggcgagcca ttcctcccgc
     6601 gggatgccta gcgtggtttc gatggacggg ctgacatcca ccatctggga tccggtcccc
     6661 aggccgtcga tgtagatcac ggcggggagc tggtcgacca gggcacgata gcgaagctcc
     6721 aggtcgcgca ggcgctcggc cgcgccgaac ggagtgtcgt cgctggatcc ggatggaggg
     6781 aagaaaccgc tcagggcggt cagcgcttcg ggtccaacgg cctcgaggtc atcctggttc
     6841 aagggggctc cgtcgtgatt cggggaagtc ctccctttca tcggctccgc tgcagccgtt
     6901 cttgaacagg ttgcggaaac ggtttgtcag caggccccag accgcatcga ccggtcgacg
     6961 tccatgcggc gaacggtcga ccgtcaacag cctcgttcgt cgggaaggag cggccggaac
     7021 cgcaggctcc atcgtcgtcc gtcgctggtc cacggggtga gttggttggc gccctcggcg
     7081 aggggttcga gcgtctgggt ccagtcttcg cccgtgaccg ttccgcagcg atacccgctc
     7141 accgcatcgt cgcggccgaa cgacgcgagg gatggttcca gcggccatgc gaccggtgat
     7201 tgggccagat cctgttcccc ttggtagtcg gccacgtaga cgcgagcgcc ggcggccgcg
     7261 taggggacgg gctcgccctg caccgaaccg tcgggaagcc acgagggcag gtccatgagg
     7321 tcctcgacca gcccgttgag ggccacgcgc gcctcccact cctccttgct catcgtcggc
     7381 ggccggtcct gcagcgccat cagggcgtag acccggacgg tggtgtcgac gccgtcggca
     7441 tggagcgtga agaccgtgtc gggagcgtcg gcgatcgcga cgctgcccat gtcggtgagg
     7501 tcgccggccg agtccaggcc ggcgtcgaag gccgcggcca cgatcgcatc cacccctgcg
     7561 ctatcgaccg tctggctctg gagcgagggc agcgccggcg gcggatagat ctcgatcatc
     7621 ggaccggtgg tgacgatcgt gccgtcgccg tacagcgaga acgccggcat gttggtgagt
     7681 tggtagtcga ccggggtgaa cccgccttcg gtggtgaccc ggagcaccag ggtggtgccg
     7741 tcttgatcga cgggtggggt cgtggagcgg gatcccgcgc cggtgtccgc cgtatcggcg
     7801 cacgcggcac ccacgagggc caggagcgct gcggcagcga cgatggaggt gcgagtcatg
     7861 gcggggtcct ttcgcgtcgg gcgagcgtgt catggctcgg acgttcgttc caggagcgtg
     7921 ggttcccgat gcaccgggta ggcttcgcgc gatggctgcg acgatgcgcg ccctgcgcaa
     7981 gaccgcccgc gagcccggcg ccgatctcgt ggagatcgcg gttcccgaac cgcgcgaggg
     8041 cgaggtgctg gtgaaggtcc acggcgcctc gatctgcgga acggacctgc acatctacga
     8101 ctggaacgaa tgggcggaca agcggatccc ccaggtgccg atgacgttcg gccacgaggt
     8161 cgccggcacc gtggtggcgg tgggtgacga ggtgcatcat ctgcgcgagg gtgtgttcat
     8221 cgccgccgag acccacatcg cgtgcggcca ctgccccacc tgccagacgg ggcgtgccca
     8281 catctgcgag aacctgaaga tcctcggggt ggacatggac ggggtgttcg ccgaatacgt
     8341 cgtcgtcccg gcgcagaacg catggatcgt gggcgagggg atcgatccgg acgtcgcctc
     8401 gatcatggaa cccttcggca atgcggtgca cgcctcgttc ggcacgggcg gaggcgagga
     8461 catcgccacc aacgcggtgg cggtgatcgg gtgcgggccg atcggcatgt tcgccgtcgc
     8521 gatcgcgagg tcgctcggcg catggaaggt gatcgcgatc gaacccaacg agttccggcg
     8581 cgcgaaggcg gccgagatgg gtgccgatgt cgtgctcgac cccatggaga ccgaccccgt
     8641 acaggcggtc atggatctga cgcggggatc gggagccgag gtggtgctcg agatgagcgg
     8701 caacgcgcgc gcgatcgacc agggaacgag gatgctggcc cgcggtggac ggatgtcgtt
     8761 gctcggcctg cccgacgggt cggtcacgct ggacctgaac gatcaggtca tcttcaagga
     8821 ggcgcggctc cacgggatca ccggccgcga gatgttccgt acctggcagc agaccacgac
     8881 gttgctctcg acggggcgcg tcgacgttgc ctccgtcatc acgcaccgct tcccgttgga
     8941 gcgattcgag gaagcgttcg cgacgacggc gtcgggcagc gccggcaagg tgatcatgtt
     9001 ccccggaggt gagggatgac cgacgatgcg ctgggctacc tgaaggacga actctccacc
     9061 ctcgaacgcg acgggttgct gttgcacccc cgcaccctgg aagggccgca gggcgcccgc
     9121 gcccgctacg acgggcgcga ggtcatcaac ctcgcgtcga acaactacct cggcctggct
     9181 aaccatcccc ggctgaacgc cgccgcgtcg aaggctgccg ccgagctcgg cgccggttcc
     9241 ggggccgtgc gcacgatcgc tggccagatg accgagcacc tggagctcga gcgccggttc
     9301 gccgccttca agcactccga ggcggccctg atgttccagt cgggcttcac ctcgaactcg
     9361 ggcacggtgg ccgcgatcct cgcgaaggaa gacgtgatcg tgtccgaccg cctcaaccac
     9421 gcctcgatca tcgacggcgc gcggctgtcg cgggccgaga tcaaggtgtt cgaccacgcc
     9481 gatcccgacc acgccgaccg gttgctcacc gaatcggccg cgccgggccg gcggcagttg
     9541 ctcatcaccg acggcgtgtt ctcgatggac ggcgacatcg cgctcctgcc cgacctcgtg
     9601 gaggtggccg agcggcacgg cgcgatcatg atggtcgacg atgcgcatgc ctccggtgtg
     9661 ctcggcacgg gggggtcggg caccgttgac cacttcgggc tccacggccg cgtggacgtt
     9721 caggtgggca cgctctcgaa agcgatcggg gtgatcggcg gcttcatcgc gggtccggcg
     9781 cacctgatcg agtggctgca gaaccgcggg cgcccctacc tgttctcgac gtcggcgccg
     9841 ccggcggtga cggccgcctg catcgctgcc ttggacgtcc tgcgcgatga acccgaacgt
     9901 ctcgagcgac tgtgggcgaa caccgcgacg ttgaaggagg gcctggcgct gctcgggttc
     9961 gacacgggga cgtcgcagac gccgatcacg ccggtcatca ccggcgaggc cgacgtcacg
    10021 cagcggttcg ccgatcgctt gttcgaagaa ggggtgttct gccccgcgat cgtgttcccc
    10081 acggtggcca agggctcggc gcgggtgcgc acgatcgtga ccgccgacca caccgacgcc
    10141 gatctgaccg aggcgttgga gatcttcggg cgcgttggcc gggagctcgg gctggtctag
    10201 cggtcctgcg cgaaccggct cttcaatccc tgcagcatca ggtcgagccc gaaatcgaac
    10261 tgctgatcga ggtcgcagtc ggccacgtac ggcatcgcgg cggccaggca cggcaggtca
    10321 tgggagggga gctggtcgag tacgtcgtcg aagtgctcgc gatcgcgttc ggcgaacgcc
    10381 agctggcgct ccatcatcac gaacccctgg atgtacccgc cgaacgcgtg gaacacctgc
    10441 atcacgtcgg ggtcgggcac gcccatgctg cgcagcacct cgagggcctt ctccatgggg
    10501 cggagggctt cgatcgagat gggggcctgg tgcttctcgg ccatgatctc gatcagcgcc
    10561 ggatgcgtgc gcatgaggtc gcgccacgcg tgggcgacgc gcacaccgtt gccgtacgga
    10621 tcgccggggt cggcgtcgaa ggcgaactcg ctgaacaccc gctgctggat ccccgcgagc
    10681 agatcctcct tgtcgcggac gtggttatag agggacatgg cctccacgcc cacttcgcga
    10741 gcgacgcgtc gcatggatac ggcgtcgagc ccttcgctgt ccatcacgcg gagcgcggca
    10801 tcgatgaccc gctcgcgcgt gaggcgctct cggggcttgg cgttcgggtc cgtcttcgtc
    10861 accatgggtt caccgaccgg gggttgacac acttacgctg tacacctaat ctcacgtaca
    10921 ccgtaagcat acaccgtacg tcaatgaggc ccagctaagg ccgcagaagg ggggaacccg
    10981 ggatgaagct caatcccgaa acgctcgcga aggcgagcag ccatcacccg tggaggacgg
    11041 tgatcgtctg ggtcctcgtg ctgttcgctg cgggtgcctc cgccgccacg ctcctcgggc
    11101 ccgcactcac gaccgacttc gacttcacga actcccccga ggccaagcag gccgaccaga
    11161 tcctgcagga gcgtcgcctc acccaggaca cgatcaccga gacgttcatc gtcaccggtg
    11221 agccgggcgc ggtggaggac cccgacttcg tcgcaggggt caacacgttc ctgaccgacc
    11281 tgaacgggtt gggcaccgat gtcttccaag cgctcccggc ggcgttcccg ctgaccgagg
    11341 agcaggccgc cgatccacag gtggcggcac tcggcccgat cccatcagcg gacgggtcgg
    11401 cggtgctgtt cacggggatc tacgccggcg atctcgacga ggcgacgccg cacttcgaag
    11461 atgtcgatgc gatccgtgag caggtctccg ggctcgaggg catcgacgag gcacacatgc
    11521 tcggccaggt gagctcctcg gaggacttca aggtcatctc cgaggaggac ctgcagttcg
    11581 gagagtcgat cggcatcgtc gccgcgatcg tggtgctgct gatcgtgttc ggggcgatcg
    11641 tcgccggcta cctgccgctg ctcctcacgg tcttcttcac gttgccgatc accctgggca
    11701 tcgtcgggct gttcggcacg ctgtgggact tcagcttctt cacccccaac ctgatctcca
    11761 tgatgggcat cgccgtcggg gtggactacg ccctgttcat cgtgtcgcga taccgcgagg
    11821 agcggcacaa tggccggaag aagaacgacg cgatccgagc gtccggcgcc acggcatcac
    11881 gcgcggtgtt cttcagtggc ttgaccgtcg tcctggcgct ggccggcatg ctcctggtgc
    11941 cgacgacgat cttccggagc ctcgccggcg gcgcgatcat cgtggtcttg gtgtcggtgg
    12001 ccgtgtcgat gacgctgctg ccggccctgc tctcggtctt gggcgagtgg gtcaactggc
    12061 cctggctgac gcggggtcgc acgctcctcg tctggctggc gatggtcgta gcggcaggtg
    12121 tggtcggcgg cgggctcggc gcggtcggtg cccccccggc gc
//
LOCUS       DATDBZ010000399         4723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1772856, whole genome shotgun sequence.
ACCESSION   DATDBZ010000399 DATDBZ010000000
VERSION     DATDBZ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772856"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VIX62_09555"
     CDS             complement(<1..1461)
                     /locus_tag="VIX62_09555"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY5860516.1"
                     /translation="MARIALGQLNLTVGDLAGNVTRMADAAARATEAGADLVVFPELA
                     ITGYPPEDLVLRKEFVQENLDALQALAGKTASGCQVIAGFVDRSEAGIHNAAALLSGG
                     GIAARYHKIQLPNFGVFDEQRYFEPGTDVTTVEVGGQPCGITVCEDAWHDGLPFARLA
                     GVPLIVNINGSPYHRGKTEERADILRDRARQTGALIVYVNAAGGQDELVFDGGSMVVA
                     PDGSVRHRASMFSEDLLIVDLTGEASFADVRPAWPVGAAEVYGALVVGLGDYVRKNGF
                     GEVVLGLSGGIDSALTATLAVDALGADAVRAVAMPSPFSSPESVEDAEACAKALGIRI
                     DTVPITPVFDAYRHSLAELFAGKPEDVTEENLQARIRGNFLMALSNKFGSMVLATGNK
                     SEYAVGYSTLYGDMAGGFAPIKDVPKTLVYELAAWRNAQPGGPSPIPERTITKPPSAE
                     LRPDQKDTDSLPAYEVLDPIIEAYVEFDMGVDDMVGG"
     gene            complement(1575..2045)
                     /locus_tag="VIX62_09560"
     CDS             complement(1575..2045)
                     /locus_tag="VIX62_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860517.1"
                     /translation="MASDDALTPALSEARQRRATLHDALVHLEIAISSPATARIPEWT
                     ELVSKEMIGVRDAFEQHVLVTEKPGGLYEEIIMRAPRLDGTVRRLKDEHPEIGEKIGV
                     MVERLEKAEIGSPSWPLDDARDDLQRLIGTVIRHRQKGADLVWEAYNVDIGGTE"
     gene            2209..2565
                     /locus_tag="VIX62_09565"
     CDS             2209..2565
                     /locus_tag="VIX62_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860518.1"
                     /translation="MSSVVGRRARIVACAATVMVLATAVPASAVTVVRGDGSRWRPAT
                     VSIDRGGAVKWRAVFRAHVVKAYGGNWSYRRSIAQGDSVRRMFNSSGTFRFYCTIHGN
                     VSGGNCTGMCGKVLVS"
     gene            2634..>4723
                     /locus_tag="VIX62_09570"
     CDS             2634..>4723
                     /locus_tag="VIX62_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5860519.1"
                     /translation="MSTTVGLDAVSEVGVDAFAPYVPRILMRQLAHAPDQPAWTLEGS
                     VVFVDISGFTKLSEKLARKGKEGAEQVTEAIESCFTELLAVAYANGGGLLKFGGDALL
                     LFFSGEGHAARAGASAVGMRTTLRTVGKINLPRAKIQLRMSVGAHSGSFDFFLVGDSH
                     RELIVAGPAWTHTVLMEHEAIAGQIRVSRDMAALLPEGCLGLAEGPGSLLIREPSGVE
                     PVEDEARPDLAAATIAGCLSGVLRAHVAAGGGAPEHRQVVTAFLRFGGTDEMLAKHGS
                     HETAAALHAMVSCVQMAADEQGVCFLGTDVDTGGGKMILTSGAPVNTGSDEERMLLTL
                     RRIADADLPLPLGIGVNRGGVFAGDIGPWYRRTYTVMGDGVNLAARLMAQAGANEIYA
                     TAEVLEHSDTRFAVTELEPFAVKGKSKPVHAWSLGEATGSRSREAPLERFSLVGRDAE
                     MAVLDAALERTSAGEGRLVQVVGEPGIGKTRLLEEVRGRSEGLILLRATCEAYTASTP
                     YMVWRELLRELLDLGYDDPDDVVLDRLYPTVANADPELLPWLPLIGRPFGIEIPATQE
                     VEMLGEEFLRARLHESVATLLDLILDEPALIEIEDVHHMDDASADLLDYIADHLDGRP
                     WLFCVSRRPGAKGFTHDVTPDSVIVEPGALSAEGAMQLGGAVAELHPLPPHALTTVVE
                     RSGGNPQFLHDLVLA"
BASE COUNT          718 a         1605 c         1649 g          751 t
ORIGIN      
        1 gccgccgacc atgtcgtcaa cccccatgtc gaactccaca taggcctcga tgatcggatc
       61 gagcacctcg taggcgggca gtgagtcggt gtccttctgg tcgggccgca actcggcgct
      121 cggcggcttc gtgatcgtgc gctccgggat cggcgacggg ccacccggct gagcgttccg
      181 ccaggccgcc agctcgtaca ccaaggtctt gggcacatcc ttgatcggtg cgaacccgcc
      241 cgccatgtcg ccgtagagcg tcgaatagcc caccgcgtat tcgctcttgt tgccggtcgc
      301 cagcaccatg gagccgaact tgttcgacag cgccatcaaa aagttgcccc ggatccgcgc
      361 ctgcaggttc tcctcggtga cgtcctcggg cttgccggcg aacagctccg cgagcgaatg
      421 acggtacgcg tcgaacaccg gcgtgatcgg cacggtgtcg atgcggatgc ccagcgcctt
      481 ggcgcacgct tcggcgtcct cgacgctctc cggtgaggag aacggcgacg gcatggccac
      541 cgcccgcacg gcatcggcgc cgagcgcgtc gacggccagg gtcgcggtca acgccgagtc
      601 gatcccgccc gacagcccca ggaccacctc gccgaatccg ttcttgcgca cgtagtcgcc
      661 caggccgacg accagcgcgc cgtacacctc cgcggcgccc accggccacg cgggccgaac
      721 gtcggcgaac gaggcctcgc cggtcagatc cacgatcagc aggtcctcgg agaacatcga
      781 ggcgcgatga cggaccgaac cgtcgggcgc caccaccatc gaccccccgt cgaacaccag
      841 ctcgtcctgg cctccggcgg cgttcacgta gacgatcaac gcaccggtct ggcgcgcccg
      901 gtcacggagg atgtcggcgc gctcctcggt cttgccgcgg tggtacggcg agccattgat
      961 gttcacgatc agcggcacgc ccgccagccg cgcgaagggc agcccgtcgt gccaggcgtc
     1021 ctcacacacg gtgatcccgc acggttggcc gcccacttcc acggtggtca catcggtacc
     1081 gggctcgaag tagcgctgtt cgtcgaacac gccgaagttc gggagctgga tcttgtggta
     1141 gcgagcggcg atcccgccac cggacagcaa cgccgctgcg ttgtggatgc cggcttcgct
     1201 gcgatcgacg aacccggcga tcacctggca cccgctcgcg gtcttgccgg ccagtgcctg
     1261 tagcgcatcc aggttctcct ggacgaactc cttacgcagg acgaggtcct ccgggggata
     1321 gcccgtgatg gcgagttccg ggaacaccac gaggtcggcc cccgcttcgg tggcccgcgc
     1381 cgccgcgtcg gccatgcgcg tcacgttgcc ggcgagatca ccgaccgtga ggttcagctg
     1441 cccgagcgcg atgcgagcca tggcggaagc ctacggcagg acgcctccaa ggggtccgcg
     1501 ctcaagcagc cgaaggcggt agcgcgaaga agtgcgctca agcagccgaa ggcggtagcg
     1561 cgaaggaaag agtcctactc ggtgccgccg atgtcgacgt tgtaggcctc ccagacgagg
     1621 tcggcgccct tttggcggtg acggatcacc gtgccgatca accgctggag atcgtcgcga
     1681 gcgtcgtcca gcggccagct cggcgagccg atctcggcct tctcgagccg ctcgaccatg
     1741 acgccgatct tctcgccgat ctcgggatgc tcgtccttca gccggcgcac cgtgccgtcc
     1801 aggcgcggcg cccgcatgat gatctcctcg tagagcccgc cgggcttctc cgtgaccagc
     1861 acgtgctgct cgaacgcgtc gcggacgccg atcatctcct tggagaccag ctcggtccat
     1921 tccgggatgc gggcggtcgc cggggaggag atcgcgatct cgaggtgtac gagcgcatcg
     1981 tgcaacgtgg cgcgccgttg gcgcgcttcg gacagagcag gggtcaacgc atcgtctgaa
     2041 gccatgcgcg cacggtagcc gaggggttcg ctcaagagca aatcccgggt tggcccggac
     2101 ggagcccgcg aactggacgc tccggcgctt ccctccggaa cgcacgaacc ggtccgggtt
     2161 cccgcccgta ctacggttgt tcacgtcgga tccgccaaag ggggccctat gtcgagtgtt
     2221 gtcggtcgcc gggcacgcat cgtcgcgtgc gcggccacgg tcatggtgct ggccacggcg
     2281 gtgccggcct cagcggtcac cgtcgtgcga ggagacggga gccgctggcg tccggcgacc
     2341 gtatccatcg atcgcggtgg tgcagtgaag tggcgcgcgg tgttccgggc ccacgtcgtg
     2401 aaggcctacg gcgggaactg gagctaccgc cggtcgatcg cgcagggtga ttcggtccgc
     2461 cggatgttca acagttctgg gaccttccgg ttctactgca cgatccacgg caacgtgagc
     2521 ggtggcaact gcaccgggat gtgcggcaag gtgctggtga gctgacgccg tccgagcgca
     2581 tcggcttcgg cctcctggtt ggtggtgggc cgcccacgcc gtaacgtcac ggtgtgtcca
     2641 cgacggtagg tctggatgcg gtctctgagg tcggggttga cgcgttcgct ccgtacgtcc
     2701 cccggatcct catgcgtcag ctcgcgcatg cgccggacca gcccgcgtgg acgctcgagg
     2761 gcagcgtcgt gttcgtcgac atctccggct tcacgaagct gtccgagaag ctggcccgca
     2821 agggcaagga gggcgccgag caggtcaccg aggcgatcga atcctgcttc accgagctgc
     2881 tcgccgtggc ctacgcgaac ggtggcggcc tgctcaagtt cggcggcgac gcgctgttgc
     2941 tgttcttctc gggcgaggga cacgctgctc gggccggcgc gtccgccgtg gggatgcgca
     3001 ccaccctgcg gaccgtcggc aagatcaacc tgccgcgcgc caagatccag ctgcgcatgt
     3061 cggtgggcgc gcacagcggt tcgttcgatt tcttcctcgt gggggattct caccgcgagc
     3121 tgatcgtggc cggcccggcc tggacgcaca cggtgctgat ggagcacgaa gcgatcgcgg
     3181 gccagatacg cgtgagccgg gatatggcgg cgctgctgcc cgaaggatgc ctcggcctgg
     3241 ccgaaggccc gggctcgttg ctcatccggg aaccctccgg ggtggaaccg gtcgaggacg
     3301 aggcccgtcc ggatctggcg gccgccacga tcgccggatg tctgtcgggc gtgttgcgtg
     3361 cccacgtggc cgcgggcgga ggcgcccccg agcaccgaca ggtggtcacg gcgttcctcc
     3421 ggttcggcgg gaccgatgag atgctcgcca agcacggttc ccacgagacc gccgcggcgc
     3481 tccacgcgat ggtgagttgc gtgcagatgg ccgccgacga gcagggggtg tgcttcctcg
     3541 gcaccgacgt ggataccggc ggcggcaaga tgatcctgac ctcgggggcg ccggtcaaca
     3601 ccgggagcga cgaggagcgc atgctgctca cgctccggcg gatcgccgat gccgacctgc
     3661 cgctgcccct cggcatcggt gtgaaccgcg gaggcgtgtt cgccggcgac atcggcccgt
     3721 ggtaccggcg gacgtacacg gtgatggggg acggggtgaa cctggccgcc cggctcatgg
     3781 cccaggccgg cgcgaacgag atctacgcca cggccgaggt gctggagcac tcggacacgc
     3841 ggttcgcggt gaccgaactc gagccattcg cggtgaaggg caagagcaag cccgtccatg
     3901 cgtggtcgct gggagaggcg acgggatcgc gatcgcgcga agccccgctt gaacggttct
     3961 cgctcgtggg gcgggacgcc gagatggcgg tgctcgacgc cgctctcgag cggaccagcg
     4021 ccggggaggg gcgcctcgtg caggtcgtcg gggagcccgg gatcggcaag acgcgcttgc
     4081 tcgaagaggt ccgtggccgc tccgaagggt tgatcctgct gcgggccacc tgcgaggcct
     4141 acaccgcgtc cacgccgtac atggtgtggc gggaactgct gcgggagctg ctcgacctgg
     4201 ggtacgacga tcccgacgac gtcgtgctcg accggctgta ccccaccgtc gcgaacgccg
     4261 atcccgagct gctcccatgg ttgccgctca tcggacggcc gttcggtatc gagatcccgg
     4321 cgacccagga ggtcgagatg ctgggcgagg agttcctgcg cgcccggttg cacgaatcgg
     4381 tcgccacgct cctcgatctg atcctggacg agccggcgct gatcgagatc gaagacgtcc
     4441 accacatgga cgacgcgtcg gccgacctgc tggactacat cgccgatcat ctcgacggcc
     4501 gcccctggct cttctgcgtc agccggcgtc cgggggcgaa ggggttcacg cacgacgtca
     4561 cgcccgactc cgtgatcgtc gaacccgggg ctctgtcggc cgagggggcg atgcagctcg
     4621 gtggcgccgt tgccgaactg cacccgctgc caccccacgc gctgacgacg gtggtggaac
     4681 gatcgggcgg caacccccag ttcctgcacg acctggtgct ggc
//
LOCUS       DATDBZ010000400         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_756303, whole genome shotgun sequence.
ACCESSION   DATDBZ010000400 DATDBZ010000000
VERSION     DATDBZ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756303"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..175
                     /locus_tag="VIX62_09575"
     CDS             23..175
                     /locus_tag="VIX62_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5860520.1"
                     /translation="MYVDDVDAVLDQLRSAGAPVVAEPEDQPWGERVAQTRDPDGNLV
                     YLGAVR"
     gene            complement(183..1229)
                     /locus_tag="VIX62_09580"
     CDS             complement(183..1229)
                     /locus_tag="VIX62_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HEY5860521.1"
                     /translation="MLATMVHPPKARPGQSVAVVAPSFAAPERFPAVHTQAMRRLTEV
                     TGLVPVEYPSTRCSSTPRQRARDLNAAFADPQIRAVLAVIGGEDQITVVPHLDPALVR
                     ADPKPFLGYSDNTNILNWLWCNGIAGFYGGSTQVHLGAGPGVDAAHAATLRAALLTAE
                     RLEITDPGESEDFGRPWDEPAALVEYGDREPTEPWTWAGPSRSVTGRTWGGCIEVIQW
                     LLAAGRFPTDPAVLDGAVLLLETSEELIPAREFGWILRALGERGMLAAVDAVLVARPP
                     GSDHDRPSSAIERAAHRAAQRDAAIEIVGRYSPDAIVCVGIPFGHTRPQLILPYGGTI
                     TIDSAARRVWADYS"
     gene            complement(1252..2028)
                     /locus_tag="VIX62_09585"
     CDS             complement(1252..2028)
                     /locus_tag="VIX62_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5860522.1"
                     /translation="MSHESFDPFAPFDLTGQVAVVTGASSGLGDRFARVLHAAGASIV
                     LAARRRDRLEALAAALPGALPIVADLSLPDDRERVIADAVERFGGVQVLVNNAGIGHT
                     VAVEDEDLDTFRAAMELNVTAVWHLSKLAAVHMTAARYGSIVNVASMLGHVGATPVKQ
                     AHYCASKGAVINLTRELGLQWARKGVRVNALCPGWFPSEMTAGMESDEASQRFIQQNS
                     PIPRMGEEHELDGALLLLASRASTFITGQSIIVDGGWTAR"
BASE COUNT          347 a          755 c          801 g          351 t
ORIGIN      
        1 cgacgaccgg ttcggctact gggtgtacgt cgatgacgtc gacgccgtcc tcgaccagct
       61 gcgctccgcc ggggcccctg tcgtcgccga gcccgaagac cagccgtggg gcgagcgagt
      121 ggcacagacg cgggaccccg atggcaacct cgtctacctc ggcgccgtgc gctgacggtt
      181 ggtcaggagt agtccgccca gactctgcgg gcggcactgt cgatcgtgat ggtcccgccg
      241 tagggaagga tcagctgggg tcgggtgtgc ccgaacggga tcccgacgca cacgatggcg
      301 tcggggctgt accgcccgac gatctcgatc gcagcgtcgc gctgggcggc gcggtgggcg
      361 gcccgttcga tcgcgctcga cggtcgatca tggtccgacc cgggcgggcg ggcgacgagc
      421 acggcatcga ccgcggcgag catgccgcgc tcgccgaggg cccgcaggat ccagccgaac
      481 tcacgagctg ggatgagctc ttcgctcgtc tcgagcagca gcaccgctcc gtcgagcaca
      541 gccgggtcgg tcgggaaccg gccggcggcg agcaaccact ggatcacctc gatacagcca
      601 ccccatgttc gcccggtgac cgatcgggac gggccggccc acgtccacgg ttcggtgggc
      661 tcgcggtcgc cgtactcgac caacgccgct ggctcgtccc atggccggcc gaagtcctcg
      721 gactcgcctg ggtcggtgat ctccaaccgc tcggcggtga gcagcgcagc gcgcaacgtt
      781 gccgcgtggg cggcgtcgac gccagggccg gctcccaggt ggacctgtgt ggagccaccg
      841 tagaaaccgg cgattccgtt gcaccacagc cagttgagga tgttcgtgtt gtcgctgtag
      901 ccgaggaacg gcttggggtc ggcgcgcacc agggcgggat cgaggtgcgg tacgacggtg
      961 atctggtcct ccccaccgat caccgcgagg acggcacgga tctgcgggtc ggcgaacgca
     1021 gcgttgaggt cgcgggcgcg ctgtctcggg gtcgaggaac atcgggtgct cggatactcc
     1081 accgggacga gaccggtcac ctcggtgagt cgccgcatcg cctgggtgtg gacagccgga
     1141 aagcgctccg gtgccgcaaa cgacggcgcg accaccgcga ccgactgccc aggtcgggct
     1201 ttcggtgggt gcaccatggt tgcaagcatg cgctcatcga accacgacga actacctcgc
     1261 cgtccacccg ccgtcgacga tgatgctctg accggtgatg aacgtgctcg cccggctggc
     1321 gaggaggagc agcgcgccgt cgagctcgtg ttcctcgccc atgcgcggga tgggtgagtt
     1381 ctgctggatg aaccgctggc tggcctcgtc gctctccatg ccggcggtca tctcgctcgg
     1441 gaaccatccg gggcacaggg cgttcacccg cacgcccttg cgggcccatt gcagacccag
     1501 ctcacgcgtg aggttgatca cggcgccttt gctggcgcag taatgggcct gcttcaccgg
     1561 cgtggcgccg acgtggccga gcatcgacgc cacgttgacg atgctgccgt agcgggcggc
     1621 cgtcatgtgg accgccgcga gcttggagag gtgccagacc gcggtcacgt tgagctccat
     1681 cgcggcgcgg aacgtgtcga ggtcctcgtc ctcgacggcg accgtgtggc cgatgccggc
     1741 gttgttgacg agcacctgca cgccaccgaa tcgctcgacc gcgtcggcga tgacgcgctc
     1801 ccggtcatcc ggaagcgaca ggtcggcgac gatcgggagc gcgccgggca gcgcggcggc
     1861 gagcgcctcg agccggtccc ggcggcgagc ggcgaggacg atggaagcgc ccgcggcgtg
     1921 gaggacacga gcgaaccgat cacctaatcc tgacgacgcg ccggtcacga cggcgacctg
     1981 tccggtcaga tcaaacggtg cgaatgggtc gaatgactca tgtgacatgg cgatgacgtt
     2041 acggggcgtg gtcacttcat tccggtttca tgaagaagtg cccgcgcggt gaatcagatg
     2101 gcgctctgcg gcgtcatata cacgtgatga cgcgcacgcc cacctccagc acagcgcccg
     2161 cgtggcgcac caccttcgac ctcgcgctgc tcgtcagtac cgccgcggcg acaatcgccg
     2221 tcgcgctcag ttggttcttc ggcgtgaccg aggc
//
LOCUS       DATDBZ010000401         4400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2360550, whole genome shotgun sequence.
ACCESSION   DATDBZ010000401 DATDBZ010000000
VERSION     DATDBZ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360550"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VIX62_09590"
     CDS             <1..326
                     /locus_tag="VIX62_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY5860523.1"
                     /translation="LFVNPAYDGMNLVAMEGPLVNRRNGVLVLSRNAGAFGRLGRQAL
                     GTNPFDLAETSEALREALEMPADERTRRARGLARTVQAHTPSNWLASQLEAVDQVRPP
                     TRRRR"
     gene            complement(336..1043)
                     /locus_tag="VIX62_09595"
     CDS             complement(336..1043)
                     /locus_tag="VIX62_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5860524.1"
                     /translation="MATPPIVLVPGFWLGAWAWDEVAGALRADGHDVTAITLPGLESA
                     DADRSSITLSDHVDAICAAVEAAGAPVVLAVHSATGFSGYAASDRIPERIAAMVYVDS
                     APGTGAADPDFADTEKPLPPQQELEAEENFDGLSAEQIETFRRRAVPVPGGVLREGVS
                     LSNDARLDVPTTMICTGYTSQEYQDAVKEGFAWLGGLTEVRNVTWVDLPTSHWPMWSR
                     PKELAGIIGDVANAHAV"
     gene            complement(1107..1853)
                     /gene="otsB"
                     /locus_tag="VIX62_09600"
     CDS             complement(1107..1853)
                     /gene="otsB"
                     /locus_tag="VIX62_09600"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY5860525.1"
                     /translation="MRATEDDAALRAFTTRDGRAGVLLDFDGTLSPIVARPELAKIRD
                     GARDAIARLVERLAIVAVISGRTPAELEELVAVPGVVLAGLYGLGEEAITLPAGVADQ
                     VTGIAATVPGTRVESKGASIAVHFRGADDPDDARGRLAPALEAVALDAGMALIAGKKV
                     WELVPGGRPLKEGAVERIVTEARLDAVLYAGDDVADQRAFEALDRLAAAGLTTLKVAV
                     HGPETPPQLAGSADIVVDGPAGLVALLRRI"
     gene            1982..3868
                     /locus_tag="VIX62_09605"
     CDS             1982..3868
                     /locus_tag="VIX62_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019667509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY5860526.1"
                     /translation="MGGDIAPWQSRQSPFPPIGEYGFLSDREVSALVGPSGSVEWLCL
                     PRPDSPSVFSAILDRGAGAFRLGPAELLVPAGRRYLPGTNVLETTWQTRSGWLIVRDA
                     LVMGPWVDTDGRREEYRRPPGDFEARHMLVRAVRCIQGQVELILNCEPVFDYGRTDAR
                     WEYTGEGYEEAVAHGGPDDPSLRLTTNMRLGMAGRAAVAFTRLNDGEAHPPPADPEEA
                     WAQIHETADFWRGWLNKGEFPDHPWRAHLQRAALTLKGLTYAPTGALIAASTTSLPET
                     PGGERNWDYRYSWVRDATFALWGLYTLGFDFEANSFFYFIADQVSDGSPLQVMYAVGG
                     ETDLTEVILDHLTGYELAKPVRIGNAAHGQAQHDVWGALLDSVFLHLRSGEFLSGSTW
                     KGLAEQVDRAIARWTEPDQGIWEIRGEPQHFTSSKIMCWVACDRGARLAEVMDKSDVA
                     QRWQAEADRIKADILDKGVDERGVFTQAYGSKALDASLLLAPLLRFLPADDPRIVATV
                     LAIADELTDDGLVLRYRTEETDDGLQGQEGTFAICSFWLVSALVEIGELDRARELCER
                     MLSYASPLGLYAEEIDPRTGRHWGNFPQAFTHLAQINALMHIIRAEEAAGGAALMGPR
                     PLGG"
     gene            complement(3840..>4400)
                     /locus_tag="VIX62_09610"
     CDS             complement(3840..>4400)
                     /locus_tag="VIX62_09610"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5860527.1"
                     /translation="AVTNLLSVAAALGVLTAVFQWGWGLSLIGLDAPGGTVPIASFVP
                     LMMFAVLFGLSMDYEVFLVSRIQQHHAEGEPPRQAVISGLGSGAHVVTAAALIMFCVF
                     SSFIINGDPTVKQFGVGLAVAVALAGLLVVTLAPALLTLFGRGVFWVPRFLDRILPHL
                     DIEGGAGTGADPGAEELATRPADEDP"
BASE COUNT          678 a         1599 c         1504 g          619 t
ORIGIN      
        1 tgctgttcgt caacccggca tacgacggca tgaacctggt cgccatggag ggccccctgg
       61 tgaaccgtcg caacggcgtg ctggtgctct cccgcaacgc cggagcgttc ggccgcctgg
      121 gccggcaggc gctgggtacg aacccgttcg acctcgccga gacgtccgaa gcgctgcggg
      181 aggccctgga gatgcccgcc gacgaacgca cccggcgagc tcggggactg gctcgcaccg
      241 tgcaggcgca caccccgtcg aactggctcg cgagccagct cgaggccgtg gaccaggtgc
      301 ggccgcccac gcgccgccgt cgctgatccg accggtcaga cggcgtgcgc gttcgcgacg
      361 tcgccgatga tcccggcgag ctctttcggg cgagaccaca tcggccagtg gctcgtcggc
      421 aggtcgaccc acgtgacgtt gcgaacctcg gtgaggccgc cgagccacgc gaatccttcc
      481 ttgacggcgt cctggtactc ctgcgacgtg taccccgtgc agatcatcgt ggtcggcaca
      541 tcgaggcgcg catcgttgct cagcgagacg ccttcgcgca gcacgcctcc cggcacggga
      601 acggcccgcc ggcggaaggt ctcgatctgc tcggcggaga ggccgtcgaa gttctcttcg
      661 gcctccagtt cctgctgcgg aggcagcggc ttctcggtgt cggcgaagtc tgggtcggca
      721 gcacccgtgc ccggcgcgga atcgacgtag accatggcgg cgatacgctc cgggatgcgg
      781 tcgctcgcgg cgtagccgga gaacccagta gcgctgtgca cggcgagcac gacgggggct
      841 cccgccgcct cgaccgctgc gcagatcgcg tcgacgtggt cggacagggt gatcgaggac
      901 cggtcggcat cggccgactc gagaccggga agcgtgatgg cggtgacgtc atggccgtcg
      961 gcacgcaacg cgccggcgac ctcgtcccac gcccatgcac cgagccagaa tccgggaacc
     1021 aagacgatcg gtggggtagc catggtccaa accctaacgg cgcgcggcga catccgcgtc
     1081 ggacggcatc ggtcccgacg gccctgctag atccggcgga gcagcgccac cagcccggcg
     1141 gggccgtcga cgacgatgtc ggccgatccc gcgagttggg gtggcgtctc cggtccgtgc
     1201 accgccacct tcaaggtggt gagcccggcg gcggccaggc ggtcgagggc ctcgaaggcc
     1261 cgctggtcgg cgacatcgtc gcccgcgtac agcaccgcgt cgagtcgggc ctccgtgacg
     1321 atccgctcga ccgcgccttc cttgagcggg cggccaccgg gcaccagttc ccagaccttc
     1381 ttgcccgcga tcaacgccat gccggcatcg agggccaccg cctccaacgc cggagcgagc
     1441 cgccctcgtg cgtcgtcggg atcgtcggca ccccggaaat gcaccgcgat cgaggcgccc
     1501 ttggactcca ccctggtgcc cggcacggtg gcggcgatcc cggtcacctg gtcggcgacc
     1561 cctgccggaa gcgtgatggc ctcctcgccc aggccgtaca gcccggccag gacgacgccg
     1621 ggcacggcga cgagttcctc gagctcggcg ggggtccgcc cggagatcac cgcgacgatc
     1681 gcgagacgct cgaccagccg ggcgatcgca tcgcgggcgc cgtcgcggat cttggcgagc
     1741 tcgggcctgg ccacgatggg gctgagggtc ccatcgaaat cgagcagcac ccccgcccgg
     1801 ccgtcccgcg tggtgaacgc ccggagcgcg gcgtcgtcct cagtcgcgcg catcgtcatc
     1861 gctcccatgc atgcctagca acatacgccg gaaatccagg tgacagctga cctcggggct
     1921 acactcgttc cccgtgagca gcgaggtcgt gcccttcccc gggaaccctt cgaccccgcc
     1981 ggtgggcggt gacatcgccc cctggcagtc gaggcagagt cccttccccc cgatcggcga
     2041 atacgggttc ctgtccgacc gcgaggtctc ggcgttggtg gggcccagcg ggagcgtcga
     2101 gtggctctgc ctccctcgcc ccgacagccc cagcgtgttc agcgccatcc tcgaccgggg
     2161 agccggcgcg ttccggctcg ggcctgccga gctgctggtg cccgccggtc gtcgctacct
     2221 tccgggcacc aatgtcctgg agaccacctg gcagacacgg tccggttggc tcatcgtgcg
     2281 cgacgcgctg gtgatgggcc cgtgggtcga caccgacggc cgtcgcgagg agtaccgccg
     2341 tccgcccggc gacttcgaag cgcggcacat gttggtgcgc gcggtccgct gcatccaggg
     2401 acaggtcgag ctgatcctga actgcgagcc ggtgttcgac tacgggcgca ccgacgcgcg
     2461 gtgggagtac accggcgagg gctacgagga ggcggtggcc cacggaggtc ccgacgatcc
     2521 ctccctgcgt ctcaccacga acatgcgcct gggcatggcg gggcgtgccg ccgtcgcgtt
     2581 cacccgtctg aacgacggcg aggcgcaccc accgccggcc gaccccgagg aagcgtgggc
     2641 gcagatccac gagaccgccg acttctggcg cggctggctc aacaagggcg agttccccga
     2701 ccacccgtgg cgcgcgcacc tgcaacgggc tgccctgacg ctgaaaggcc tcacgtacgc
     2761 tcccaccggc gcgctgatcg ccgcctccac cacctcgttg cccgagacgc cgggcgggga
     2821 gcggaactgg gactaccgct acagctgggt gcgcgatgcc acgttcgcgc tctggggcct
     2881 ctacaccttg ggcttcgact tcgaagcgaa cagcttcttc tacttcatcg ccgatcaggt
     2941 ctccgacggg tcgccgctgc aggtgatgta cgcggtgggc ggcgagaccg acctcaccga
     3001 ggtgatcctg gaccacctca cgggctacga actcgccaag cccgtgcgca tcggcaacgc
     3061 cgcgcacggc caggcccagc acgacgtgtg gggcgcgctg ctcgactcgg tgttcctcca
     3121 cctgcgcagc ggggagttcc tgtcgggcag cacgtggaag gggctcgccg agcaggtcga
     3181 ccgcgcgatc gcccgctgga ccgaacccga ccaggggatc tgggagatcc ggggcgaacc
     3241 gcagcacttc acctcctcga agatcatgtg ctgggtggcg tgcgaccggg gcgccaggct
     3301 cgccgaggtc atggacaagt ccgacgtggc ccagcgctgg caggccgagg ccgatcgcat
     3361 caaggccgac atcctggaca agggcgtcga cgagcgcggc gtgttcacac aggcctacgg
     3421 atcgaaggcg ctcgacgcct cgctgctgct ggcgcccctg ctccggttcc tgccggccga
     3481 cgacccgcgg atcgtggcca ccgtgctcgc gatcgccgat gaactcaccg acgacggact
     3541 ggtgctgcgc tatcggaccg aggagaccga cgacggcctc cagggccagg agggcacgtt
     3601 cgcgatctgt tcgttctggc tggtctcggc gttggtggag atcggcgagc tcgatcgagc
     3661 ccgcgagttg tgcgagcgca tgttgtccta cgcgagcccg ctcggcctct acgccgaaga
     3721 gatcgacccg cgcaccggcc gccactgggg gaacttcccc caggcgttca cccacctggc
     3781 gcagatcaac gcactgatgc acatcatccg cgccgaagag gcggcgggcg gcgcggcgct
     3841 catgggtcct cgtccgctgg gcgggtagcg agctcctcgg ctcccgggtc cgcccccgtg
     3901 cccgcgccgc cttcgatgtc gaggtggggc aggatccgat cgaggaaccg cggcacccag
     3961 aacacccccc gaccgaacag cgtcagcagc gcgggcgcca gcgtcaccac caacaggccg
     4021 gccagggcga ccgccaccgc caggcccacc ccgaactgct tcaccgtggg gtcgccgttg
     4081 atgatgaagc tggagaacac gcagaacatg atcagcgccg ccgcggtcac cacgtgtgcg
     4141 cccgatccca gccccgagat caccgcctgg cgcggcggct cgccctcggc gtggtgctgc
     4201 tggatgcggc tcaccaggaa cacctcgtag tccatcgaga gtccgaacag caccgcgaac
     4261 atcatcagcg gcacgaagct cgcgatcggc acggtgcccc cgggcgcgtc caacccgatc
     4321 agcgacagac cccaccccca ctggaagacc gcggtcagta cgccaagggc ggccgccacc
     4381 gacaacaggt tcgtgaccgc
//
LOCUS       DATDBZ010000402         2695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2187950, whole genome shotgun sequence.
ACCESSION   DATDBZ010000402 DATDBZ010000000
VERSION     DATDBZ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187950"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VIX62_09615"
     CDS             complement(<1..592)
                     /locus_tag="VIX62_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860528.1"
                     /translation="MHVILATLIILVASTLAIVGILVVRRRAPDGGYFTNGDRAAGVF
                     GVLATGFTLLLGFVVFLAFTKYDESRAGSEAEALVLVQQFETAQLMPVEVRSRLSGEL
                     ICYGRSVVGAEWPAMRSGRGAELINPWAIALFRSLETVQPEAPSEQSAYDAWLDHTST
                     REEARRDRLHAAEGIVPQSVWIVLYFSAALILLYVLF"
     gene            complement(650..1285)
                     /locus_tag="VIX62_09620"
     CDS             complement(650..1285)
                     /locus_tag="VIX62_09620"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5860529.1"
                     /translation="MARVRQKNGKDRHQEILEAAARVITDRGLAETRIQDIAERCGVS
                     PGLILYYFESKDRLLVEALTYANDKYYLAQARDLRRLDSARDQLWRLIDLSVPGLLPD
                     FERLEEWALWVEIWVRALRDPALAKEREVLDRRWRQSIADIVRQGRSTGEFVEGEDAD
                     EIGMKLGALIDGLAIQVLMNDTQVGPEHMQRMCQEVAASLIGFELAPATPA"
     gene            1509..>2695
                     /locus_tag="VIX62_09625"
     CDS             1509..>2695
                     /locus_tag="VIX62_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5860530.1"
                     /translation="MASNTYDAIVIGGGHNGLTSGAYFARHGARTVVLESRNKTGGAA
                     DTSAPFAEHPEINVTTYSYVMSLMPPTIIRELNLKRHGYDVTPFGPYFQAFPDGRAIT
                     VYADDAKKSYDSIAQFSKKDADTLPKWEAWMKGVSDVLGPLLLSVPPHVGSMKLGDLL
                     PQLQTAWNMRKLGQRGVADVTRLFTMSVTDLLDDWFESDAIKAMLTVNGVIGTWAGPD
                     EPGTAYVMLHHSIGDVGDGHLGSWGFQQGGMGAVSDSIRRSAESFGCEVRTEAKVAKI
                     LTRGGRAVGVALENGEELRAPVVVSCIHPKIGFLDLLDRNELPADFVWDIERWKTRSG
                     TVKVNVAISELPNFTAMPGTDQQDHHTGSVELCFSPQYAERAFQDAHIDRKPAVAPFV
                     DGT"
BASE COUNT          471 a          928 c          888 g          408 t
ORIGIN      
        1 agaacagcac gtagagcagg atcagcgcgg ccgagaagta cagcacgatc cacaccgact
       61 gaggcacgat gccctcggct gcgtgcaacc ggtcacgccg tgcctcctcc cgggtgctcg
      121 tgtggtccag ccacgcgtca taggcgctct gctcgctcgg ggcctcgggc tgcacggtct
      181 ccaagctgcg gaagagcgcg atcgcccatg ggttgatcag ctcggcccca cggcccgacc
      241 gcatcgccgg ccactccgcg cccacgacgg agcggccgta gcagatcagc tcgcccgaga
      301 gcctcgatcg cacctccacg ggcatcaact gcgccgtctc gaactgctgc acgagcacga
      361 gcgcctcagc ctccgaccct gcccgggact cgtcgtactt cgtgaacgcc aggaagacca
      421 cgaagcccag cagcaaggtg aatcctgtcg cgagcacgcc gaagacaccc gctgcccgat
      481 cgccgttcgt gaagtaccca ccgtcgggcg cccgtcgtcg caccaccagg atgccgacga
      541 tcgcgagcgt gctcgcgacg aggatgatga gcgttgccag gatgacgtgc atcggactcc
      601 tcccgccgtc cgcgcaccct atcccgtcca gggcagggca ggaggagtct caggcggggg
      661 tcgcgggggc gagctcgaag ccgatcaggc tcgcggccac ctcttggcac atgcgctgca
      721 tgtgctcggg cccgacctgt gtgtcgttca tcagcacctg gatcgcgagg ccgtcgatca
      781 gtgcgccgag cttcatgccg atctcgtcgg cgtcttctcc ttcgacgaac tcgccggtcg
      841 agcggccctg gcgcacgatg tcggcgatcg actgacgcca gcgccggtcc agcacttccc
      901 gttctttggc gagcgcgggg tcgcgcaggg cccggaccca gatctcgacc cacagcgccc
      961 attcctcgag gcgttcgaag tcgggcaaca acccgggcac cgagaggtcg atcaaacgcc
     1021 agagctggtc gcgcgccgag tcgagcctgc gcaggtcgcg cgcctgcgcg aggtagtact
     1081 tgtcgttggc gtaggtgagc gcctcgacga gcagacgatc cttcgactcg aagtagtaga
     1141 ggatcagccc cggcgagacg ccgcaccgtt cggcgatgtc ctggatgcgg gtctcggcca
     1201 gcccgcggtc ggtgatcacg cgcgcggcgg cttccaggat ctcctggtga cggtccttgc
     1261 cgttcttctg ccgtacgcga gccaacgggc cctcctcctg agtgcctggt gtcctgaacg
     1321 ccgcacagcc agaggccgag agcgcccgtt ccttgtatga acgttcaggc aactgtaacg
     1381 gcatgacctg ggaagtcaaa accggcacca tggccacacc gagttgactg gtgattcaaa
     1441 ctcaggccat actgcccggg ttccggagac gtcaaggggc ccactcgagg ggaaggacgc
     1501 gtacggctat ggcttcgaat acctacgacg cgatcgtgat cggcggcggt cacaacggac
     1561 tcacgagcgg ggcctacttc gcccgccacg gcgcccgcac ggtcgtgctg gagagccgca
     1621 acaagaccgg cggcgcggcc gacacgagcg cgccgttcgc cgagcacccc gagatcaacg
     1681 tcacgaccta cagctacgtg atgtcgctga tgccgccgac gatcatccgc gagctgaacc
     1741 tgaagcgcca cgggtacgac gtcacgccgt tcggtccgta cttccaggcg ttccccgacg
     1801 gccgcgcgat cacggtctac gccgacgacg cgaagaagag ctacgactcg atcgcgcagt
     1861 tctcgaagaa ggacgccgac acgctgccca agtgggaagc ctggatgaag ggcgtgagcg
     1921 acgtgctcgg cccgctgctg ctgagcgtgc cgccgcacgt gggctcgatg aagctcggcg
     1981 acctgctgcc acaactgcag accgcctgga acatgcgcaa gctcggccag cgcggcgtgg
     2041 ccgacgtgac ccggttgttc acgatgagcg tgaccgacct gctcgacgat tggttcgagt
     2101 ccgacgcgat caaggcgatg ctcacggtga acggcgtgat cggcacgtgg gccggccccg
     2161 acgagcccgg caccgcctac gtgatgctgc accactcgat cggcgacgtc ggcgacggcc
     2221 acctcggcag ctggggcttc cagcagggcg gcatgggcgc ggtgagcgac tcgatccgtc
     2281 gcagcgccga gtcgttcggc tgtgaggtgc gcaccgaggc gaaggtcgcg aagatcctca
     2341 cgcgaggcgg tcgcgctgtc ggcgtggcgc tcgagaacgg tgaggagctg cgggcgccgg
     2401 tcgtcgtgag ctgcatccac ccgaagatcg ggttcctcga cctgctcgat cgcaacgagc
     2461 tgcccgccga cttcgtctgg gacatcgagc gctggaagac gcgttccggc acggtgaagg
     2521 tcaacgtggc gatcagcgag ctgccgaact tcacagcgat gcccggcacg gaccagcagg
     2581 accaccacac cggctcggtc gagctctgct tctccccgca gtacgcggag cgggcgttcc
     2641 aggacgcgca catcgaccgc aagcccgccg tcgcgccgtt cgtcgacggc acgat
//
LOCUS       DATDBZ010000403         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1350934, whole genome shotgun sequence.
ACCESSION   DATDBZ010000403 DATDBZ010000000
VERSION     DATDBZ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350934"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VIX62_09630"
     CDS             <1..415
                     /locus_tag="VIX62_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5860531.1"
                     /translation="ESAWRQPATGTWPDWMDEWEIPFAETIDRAKKYVVSSTLSGVDW
                     NAELVRGDLGQAVQRLKQEPGEGLFVGGVTLPLALADVGLIDEYEFLVQPVLAGHGPT
                     LLAGLRERIQLELVNRHEFRSGAVAVRYRPARVTA"
     gene            466..594
                     /locus_tag="VIX62_09635"
     CDS             466..594
                     /locus_tag="VIX62_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860532.1"
                     /translation="MRATCRAAAPIAEVAETLNRRFVVLTAVGSFVGRSSAVCWSA"
     gene            complement(980..1570)
                     /locus_tag="VIX62_09640"
     CDS             complement(980..1570)
                     /locus_tag="VIX62_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5860533.1"
                     /translation="MRAAAGVAMVLASLEEVRRRNVPPYEQRVFRVANDAPDAIRIPV
                     RSVMQAGTFVTVPIAAVLAAATGHRRLAVDLATAGTLAWLGAKALKPLGGRGRPESVM
                     TDVHVRERIAGDLGWVSGHTTVATALAATAWPALPRSVRPALVAVVATTAFGRMYVGA
                     HLPLDLLGGAGLGLVLSAMVPPAVCPRQESNLRHPV"
     gene            1728..>2089
                     /locus_tag="VIX62_09645"
     CDS             1728..>2089
                     /locus_tag="VIX62_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEY5860534.1"
                     /translation="MPFEFDVVEVSPLDPNAEQHMVPMRDGVHLATDVYLPADVDRGP
                     AVLVRLPYDKCGRYTFMPRIAPSFTARGYTFVVQDVRGKFRSEGETLAFVHEIDDGYD
                     TLEWVSSQSWCDGNVGMWG"
BASE COUNT          301 a          691 c          754 g          343 t
ORIGIN      
        1 ggagtcggcg tggcggcagc cggccacggg cacgtggcct gactggatgg atgagtggga
       61 gatcccgttc gccgagacca tcgaccgggc gaagaagtac gtcgtgtcga gcacgctgag
      121 cggggtcgat tggaatgccg aactggtgcg aggcgacttg gggcaagcgg ttcagcgact
      181 caagcaggag cctggcgagg gcctgttcgt gggtggcgtg acgctgcccc tggcgttggc
      241 agacgtggga ttgatcgacg aatacgagtt ccttgtgcag ccagtccttg ccggacacgg
      301 gccgacgttg ctcgccggtc tgcgcgagcg catccagctc gagctcgtga accgccatga
      361 gttccggtcg ggggcggtcg ccgtgcgata ccggccggcg cgagttacgg cctgacgtga
      421 tttgtcctgg cccgttggcc gctaggtggt tctgggcgga aggcgatgcg ggcgacgtgc
      481 cgtgcggcgg cgcccatcgc cgaggtcgcc gagaccctca accggcggtt cgtggtgttg
      541 acggcggtcg gctcgttcgt cggacgctcg tcggcggtct gctggtcggc gtgatccccg
      601 acggctgctg atcttccttc tcgggtctgc cagggggagc gcctacaaag tctggcgcca
      661 cgacgtcccc gccccagcgc cggcgccggc ctgacggagg gatcagaggc ctgggtccaa
      721 cttgctcggt cgagaggctt cctcggcgcg gcttcgcctc gtcgagcggg tgagctcgag
      781 agcgggacca catgaggctg atcacgacga tcatcgaccg tcgccgagaa ggtcggcttc
      841 gcaaactttc ggatcacggg gctccgccga tctcgctcaa cggtgcagct agtggcccca
      901 atctggcggt ggcgccaata ttgcgccgac gcgattcgtg cgtaccggct ggcgcatgat
      961 cccggaggcg ccacgtttcc taaaccgggt gtcgcaggtt cgattcctgc cgggggcaca
     1021 ctgccggggg caccatcgcg gacaggacga gtccgaggcc ggcgccgccg aggagatcga
     1081 gcggaaggtg cgccccgacg tacattcgac cgaacgcggt ggtcgccacg acggcgacca
     1141 gcgcgggtcg gacagatcgt ggcagcgccg gccacgcggt cgcggcaagg gcggtggcga
     1201 ccgtcgtgtg gccggagacc catccgagat ctcctgcgat ccgctcacgc acgtgcacgt
     1261 cggtcatcac gctctcgggt cggccgcgcc ccccgagcgg tttcagcgcc ttcgcgccga
     1321 gccatgccag cgtgcctgcg gtcgcgaggt cgacggcgag tcgccggtgt cccgtggctg
     1381 cggcgaggac ggcggcgatc ggcaccgtga cgaacgttcc cgcctgcatc accgagcgga
     1441 ccgggatgcg gatggcatcg ggcgcgtcgt tcgcgacgcg gaacacgcgt tgctcgtagg
     1501 gcgggacgtt ccgccgcctc acttcctcca ggctcgccag caccatcgcg acaccagcgg
     1561 cggcacgcat ccctcggccc tccaatgccc ccacgccagc acccgccttc ccgggccacc
     1621 gcctccaccg gcgggcgctg cagtctacgc cagcggtgct gacgacccac ggcgatgcgt
     1681 acgctctcgg ggccgccacc gtcggcgatc cgaggaggag agggatcgtg cccttcgaat
     1741 tcgatgtcgt cgaggtgtct ccgctcgatc cgaacgccga gcagcacatg gttccgatgc
     1801 gcgacggcgt gcacctcgcg accgacgtct acctccctgc ggatgtcgac cgtggtcctg
     1861 cggtcctggt ccgtctcccg tacgacaagt gcgggcgcta caccttcatg ccccggatcg
     1921 caccgtcgtt cacggcccgg gggtacacgt tcgtcgtgca ggacgtgcgt gggaagttcc
     1981 gctcggaggg ggagaccctc gcgttcgtgc acgagatcga cgacgggtac gacacgctcg
     2041 agtgggtgtc ctcgcagtcg tggtgcgacg gcaacgtggg catgtgggg
//
LOCUS       DATDBZ010000404         2564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_110937, whole genome shotgun sequence.
ACCESSION   DATDBZ010000404 DATDBZ010000000
VERSION     DATDBZ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110937"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1225)
                     /gene="sucC"
                     /locus_tag="VIX62_09650"
     CDS             complement(11..1225)
                     /gene="sucC"
                     /locus_tag="VIX62_09650"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY5860535.1"
                     /translation="MKEARSRAPKEQQMDIHEYQAKQLLRSYRVTTPRGEVVYTSKAA
                     ARVAEELGGQRWVVKAQIHAGGRGKAGGVKLGNSPGKIAEIADQMLGSTLVTAQTGPE
                     GQRVSRVLVERASRIEREFYLSLVVDRDSQRIVVIASAEGGVDIEEVAASDPGAIHTE
                     YVDPGVGLLDFQCRKVATAIGLGNRTASFTRLLKRMYRLFRDLDCLMLEVNPFIETED
                     GDLVALDAKMSFDDNALFRQGDVVELRDFEEEDPKEVEATGHGLNYIALDGSVGCIVN
                     GAGLAMGTMDAIVHHGGRPANFLDVGGGASPEKIANAFRIVLQDPNVQAILVNIFAGI
                     NRCDWIAQGVVQATRDQAIGVPVIVRLAGTNVEEGRKILDESGLALIQADDLDDAAAK
                     AVHAVSEQGVPA"
     gene            complement(1255..2226)
                     /locus_tag="VIX62_09655"
     CDS             complement(1255..2226)
                     /locus_tag="VIX62_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019530832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY5860536.1"
                     /translation="MTRKDRLHRSELAVPGTNVRAMEKAATLDADVVFLDLEDAVAPD
                     DKEAARGNIIEALGANDWTRTTVSVRINGLDTHWCYRDVVDVVEARGQVLDTVLVPKV
                     GSPSDVEFVATLLDQIEQRNGWEPGRIGIHILIETAAGMANVEAISRARPDRLEAMVF
                     GVADYAASVRARTTNIGGANPGYSVLTDPLPQTGAREIHWGDQWHFGISRMVAACRAE
                     GLRPIDGPFGDFNDPDGYRSAARRAAALGCEGKWAIHPSQVELANEVFSPGEDEVDRA
                     RRILQAMEDAAKEGKGAVSLDGRLIDAASIRMAENLIRQVEQITRRS"
     gene            complement(2235..>2564)
                     /locus_tag="VIX62_09660"
     CDS             complement(2235..>2564)
                     /locus_tag="VIX62_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY5860537.1"
                     /translation="ALEEGLQNRWARHEDAGRYFQQQMRDRGYTFLSDPDHQLWELSA
                     IDVPEGVDGKDIQSTILRTFNIEVGGGLGPTAPPIWRVGLMGVNANRETADRVLAAFD
                     EVLPRSA"
BASE COUNT          362 a          925 c          833 g          444 t
ORIGIN      
        1 acgaacacgc tcatgccggc accccttgct cgctcaccgc gtgcacggcc ttggccgcgg
       61 cgtcgtcgag gtcgtcggcc tggatcaggg ccaggccact ctcgtccagg atcttccggc
      121 cttcctccac gttggtgccg gccaaccgga cgatcaccgg gacgccgatc gcctggtcgc
      181 gcgtcgcctg caccacgccc tgcgcgatcc aatcgcaccg gttgatgccc gcgaagatgt
      241 tcaccaggat cgcctgcacg ttcgggtcct gcagcacgat ccggaaggcg ttcgcgatct
      301 tctcgggact ggcaccacct cctacgtcga ggaagttcgc cgggcgcccg ccgtggtgca
      361 cgatcgcgtc catcgtcccc atcgccaggc cggcgccgtt cacgatgcag cccaccgagc
      421 cgtcgagcgc gatgtaattg agcccatggc cggtggcctc gacctccttg gggtcttcct
      481 cctcgaagtc gcgcagctcc accacgtcgc cctggcggaa caacgcgttg tcgtcgaagc
      541 tcatcttcgc gtcgagggcg accagatcgc cgtcctcggt ctcgatgaag gggttcacct
      601 cgagcatcag acaatccagg tcgcggaaga gccggtacat gcgcttgagc agccgcgtga
      661 acgaggcggt gcggttgccc agtccgatcg cggtcgccac cttgcggcac tggaagtcca
      721 gcaatccgac cccggggtcc acgtactcgg tgtggatcgc gccggggtcg gaggcggcga
      781 cctcctcgat gtcgacgccg ccctcagcgc tggcgatcac cacgatccgc tgcgaatcgc
      841 ggtccaccac cagcgacagg tagaactccc gctcgatccg cgaggcacgt tcgaccagca
      901 cgcggctgac ccgctggccc tcgggaccgg tctgtgcggt gaccaacgtg gagcccagca
      961 tctgatcggc gatctcggcg atcttgccgg gggagttgcc cagcttcacg ccaccggcct
     1021 tgccgcgccc acccgcatgg atctgggcct tcaccaccca tcgctggccg ccgagctcct
     1081 cggcgacgcg agcggccgcc ttcgacgtgt agaccacctc gcctcgaggc gtggtcaccc
     1141 ggtagctgcg aagcagctgc ttggcctggt actcgtggat atccatctgc tgctccttcg
     1201 gtgcccggct gcgggcttcc ttcaggcgcg accggatgcg gtcgtgccgg tcgttcacga
     1261 cctgcgggtg atctgctcca cctggcggat caggttctcc gccatccgga tcgaggcggc
     1321 gtcgatcagc cgtccgtcga gcgagacggc gccctttccc tccttcgctg cgtcctccat
     1381 ggcctggagg atcctgcgcg cgcggtcgac ctcgtcctca ccaggggaga agacctcgtt
     1441 cgccaactcg acctgcgacg ggtggatcgc ccatttgccc tcgcagccca gtgccgcggc
     1501 gcgcctcgcc gcgctgcggt agccgtccgg gtcgttgaag tcgccgaagg gaccgtcgat
     1561 cggacgcagg ccttcagcgc ggcacgccgc caccatccgg ctgatcccga agtgccactg
     1621 gtcgccccag tggatctcgc gcgcgcccgt ctgcggcaac ggatcggtga gcacggagta
     1681 gccggggttg gccccgccga tgttcgtcgt gcgcgcacgg accgaagccg cgtagtcggc
     1741 gacgccgaac accatggcct cgagccggtc gggccgagcc cggctgatcg cctcgacgtt
     1801 ggccatcccc gcggcggtct cgatcaggat gtggatgccg atgcggccgg gctcccagcc
     1861 gttgcgttgc tcgatctgat ccagcagcgt cgccacgaac tcgacgtcgg agggcgatcc
     1921 caccttgggc accagcacgg tgtcgagcac ctggccgcgc gcctccacca cgtcgacgac
     1981 gtcgcggtag caccagtggg tgtcgagccc gttgatgcgg acgctgacgg tcgttctcgt
     2041 ccagtcgttg gcaccgagcg cctcgatgat gttgccgcga gccgcttcct tgtcgtcggg
     2101 cgccaccgcg tcctcgaggt cgaggaacac gacgtcggcg tcgagcgtgg cggccttctc
     2161 catggcgcgc acgttcgtgc cgggaacggc gagttcgctt cggtggagcc ggtccttgcg
     2221 tgtcatgagg gcacctacgc tgagcggggc aggacctcgt cgaaagccgc cagcacccgg
     2281 tcggcggtct cacggttggc gttcacgccc atcaggccca cgcgccagat cgggggcgcg
     2341 gtcggcccga ggccgccgcc cacttcgatg ttgaacgtgc gcaggatcgt cgactggatg
     2401 tccttgccgt ccacgccctc gggcacgtcg atcgcgctca attcccacag ctgatggtcc
     2461 ggatccgaca ggaacgtata cccacggtcg cgcatctgct gttggaagta gcggccggca
     2521 tcctcgtggc gtgcccaccg gttctgcagg ccctcctcga gggc
//
LOCUS       DATDBZ010000405         3673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2591498, whole genome shotgun sequence.
ACCESSION   DATDBZ010000405 DATDBZ010000000
VERSION     DATDBZ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591498"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VIX62_09665"
     CDS             <1..186
                     /locus_tag="VIX62_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY5860538.1"
                     /translation="DTLDVFPGETYEIAFVADNPGIWSDHCHNLPHAVDGLIAHVMYE
                     GVQTPFTINGDAGNHPE"
     gene            457..1467
                     /locus_tag="VIX62_09670"
     CDS             457..1467
                     /locus_tag="VIX62_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007296021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY5860539.1"
                     /translation="MAALGAASGALAQDSGDHDGSGSGHGSMDMTGSTEMSATSHVGH
                     AGFPTGSVPPARAGIDPTAILRDFDTGTVSTLPNGQVLREWEITAIDVDIEIAPGVIF
                     PGWTYNGRIPGPTLWCREGDALRIRFTNAGSHPHTMHFHGVHTAEMDGTPGVGAGSIA
                     PGATFSYEFDAVPFGTHLYHCHQSPLAPHIAKGLYGGFIIEPKSGREPADDEMVMVMN
                     GYNTDGGDDNEFYSVNGLPFHFMDHPVQVKRNALVRIHLINVLEYDPINSFHVHGNFF
                     HYYPTGTMLTPSEYTDTVSQVQGQRGILDIRFPYTGKYMFHAHKTEFAELGWMGFFEV
                     SE"
     gene            1464..2687
                     /locus_tag="VIX62_09675"
     CDS             1464..2687
                     /locus_tag="VIX62_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY5860540.1"
                     /translation="MTASRDTTTQEHDVKSGAQRMPRWLLGAGPLALIALLIGLFTLL
                     NAPGITGAGDRVPEEAVAVENIRLEPGEIILFVRNEGVDPVTIAQVNVSDYFAQFTQT
                     TAEMAPLQANTITVSYNWVAGDPYEIALITSAGGKISADIDAAALSPEQGASFFGLMI
                     LLGVYVGVIPIGLGMLWMPFVRRSSRGWIQGLLAFTVGLLVYLTIDATLEGLELVADN
                     AAFGGSLTVLLGAFIAYISLEGIEGMILRSRRTTPGDRALSPQMLALLIAVGIGLHNL
                     GEGLAIGSAYAVGSLALGASLVIGFAIHNTTEGLAIVTPLAAQRPRIRRLAVLGLIAG
                     APAILGALIGATVTQPTLAALLFGIGAGAVGQVAVKLLPMLKDDTGRILTPLTAGGII
                     AGIAIMFATGLLVTI"
     gene            complement(2743..3207)
                     /locus_tag="VIX62_09680"
     CDS             complement(2743..3207)
                     /locus_tag="VIX62_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY5860541.1"
                     /translation="MSGQRKFRTTVAVDRQGRTLVPIPFDPDEPWGAKPRHPVTGSVA
                     GRPMRGVIEKREGGPVIALGPAWCRVALRDGAEVEVVLEPEGPQRADLAPDIATALDA
                     APDAAAFFDSLAQFYRRAWLRWIDSTKRQPDQRPIRIAEMVLLLQQGHKERP"
     gene            complement(3292..>3673)
                     /locus_tag="VIX62_09685"
     CDS             complement(3292..>3673)
                     /locus_tag="VIX62_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860542.1"
                     /translation="LLWILTGILCILITVGAIVTALMLAGIVGLNVQFVVATPMTLVG
                     FIWLWPAATAAVGASAVPGWFLPLSVSVLLAFMVGGAVVGGAFLFSADSAMRMVLFVA
                     GGIPIALAMMAFPAWWLILASNAR"
BASE COUNT          670 a         1300 c         1112 g          591 t
ORIGIN      
        1 gacacactcg atgtcttccc cggcgaaacc tatgagatcg ccttcgtcgc cgacaacccg
       61 ggcatctgga gcgaccactg ccacaacctc ccgcacgccg tcgacggcct catcgctcac
      121 gttatgtacg agggcgtcca aacacccttc acgatcaacg gcgacgcggg caaccatccc
      181 gaatgactca ggcagggcag cggccatcaa cccacgcatc agaaacatga ctccacgccg
      241 acatccacat tgagccgccc ggctgacctc gacgatcgat ccatctcggt ggaagattta
      301 ggtacaccga aatatactgt tctcgaggga tggtggaacg tcatggacag gtcagtgccc
      361 acccgcgtga gactcagcgt aggcagcgcg ccgagaggat cggcggagct tcccagccgt
      421 cgaagcgtgc tgttgggtgc ggcggccgca attccgatgg cggctctggg tgcggcaagc
      481 ggcgcactgg cgcaggacag cggcgaccac gacggcagcg gttccggtca cgggtcgatg
      541 gacatgaccg gctcgactga aatgagcgcg accagtcatg tcgggcacgc ggggttcccg
      601 acgggctcgg tgcctcctgc gcgtgctggg atcgacccga cggcgatcct ccgcgatttc
      661 gacaccggca cggtgagcac cctgccgaac gggcaggtgc tgcgtgagtg ggagatcacc
      721 gcgatcgacg tggacatcga gatcgctccc ggggtgatct tccccggttg gacttacaac
      781 gggcgcatcc ccgggcccac gctgtggtgc cgagagggcg acgcgctgcg gatccgcttc
      841 acgaatgccg gttcgcaccc gcacacgatg cacttccacg gggtccacac cgctgagatg
      901 gatggcacgc ccggagtcgg agccgggtcg atcgctccgg gcgcgacgtt cagctatgaa
      961 ttcgacgccg tcccgttcgg cacgcacctc taccactgcc accagtcgcc gttggcgccg
     1021 cacatcgcca agggcctcta cggcggcttc atcatcgagc cgaagagcgg ccgggagccg
     1081 gccgatgacg agatggtgat ggtcatgaat ggatacaaca ccgacggcgg tgacgacaac
     1141 gagttctact cggtcaacgg cctgccgttc cacttcatgg atcacccggt ccaggtcaag
     1201 cgcaatgcgc tcgtgcggat ccacctgatc aacgtcctcg agtacgaccc gatcaactcg
     1261 ttccacgtgc acggcaactt cttccactac tacccgaccg gcaccatgct cacgccgagc
     1321 gaatacaccg acaccgtctc gcaggtgcag ggccagcgcg gcattctcga catccgcttc
     1381 ccctataccg gcaagtacat gttccacgcg cacaagaccg agttcgccga actcggctgg
     1441 atggggttct tcgaggtgag cgaatgaccg catcccggga taccacgacg caggagcacg
     1501 acgtgaaatc gggtgctcag cggatgcctc ggtggctgct cggtgctgga ccgctcgcgc
     1561 tcatcgccct gctcatcggg ctcttcacgc tcctcaacgc tccaggcatc accggcgccg
     1621 gcgaccgcgt ccccgaagaa gccgtcgcgg tcgagaacat ccgactcgaa cccggcgaga
     1681 tcatcctgtt cgtccgcaac gaaggcgtcg acccggtcac gatcgcccag gtgaatgtgt
     1741 cggactactt cgcacagttc acccagacca cggcagagat ggcgccgctc caggcgaaca
     1801 cgatcacggt gagctacaac tgggtcgccg gtgatcccta tgagatcgcc ctgatcactt
     1861 ctgccggggg gaagatctca gccgatatcg acgccgccgc gctgagcccc gagcagggcg
     1921 cctcgttctt cggtctcatg atcctcctcg gcgtctacgt cggggtcata cccatcggcc
     1981 tcggaatgct ctggatgccg ttcgtgcgac gctcgtcgcg cgggtggatc caagggcttc
     2041 tcgcgttcac cgtgggcctg ctcgtctacc tcacgatcga cgccaccctc gagggtctcg
     2101 agctcgtcgc ggacaacgcc gcattcggcg gcagcctcac cgtgctgctc ggggcattca
     2161 tcgcatacat ctccctcgag ggcatcgagg gcatgattct gcgcagccga cgcaccacac
     2221 ccggcgaccg ggcgctctca ccacagatgc tcgcactcct catcgccgtc ggcatcggtc
     2281 tgcacaacct cggcgagggt ctcgcgatcg gctccgcata cgctgtcggc tcactcgcac
     2341 tcggagcctc cctcgtcatc ggcttcgcca tccacaacac caccgaaggc ctcgcgatcg
     2401 tcaccccact cgccgcccaa cgcccgcgaa tccgccgact cgccgtcctc ggcctcatcg
     2461 ccggcgcacc ggccattctt ggcgccctga tcggagcaac cgtcacccag ccaaccctcg
     2521 ccgcgctcct cttcggcatc ggcgcaggag ccgtcggcca ggtcgccgtg aaactgctgc
     2581 cgatgctgaa ggacgacacc ggccgcatcc tcaccccact gaccgctggc gggatcatcg
     2641 ccggcatcgc gatcatgttc gcgaccggcc tactggtcac gatctagagg gacgaagaca
     2701 cgaggagggc gttgcgtacc ctccgcgagg gcgacgagtc cgtcaaggac gttccttgtg
     2761 tccttgctgc agcaggagaa ccatttccgc gatccggatc ggtcgctgat ccggttggcg
     2821 cttcgtcgag tcgatccacc gtagccatgc ccttcggtag aactgcgcga gcgagtcgaa
     2881 gaacgccgcc gcatccggag ccgcatcgag tgccgtggcg atgtccgggg cgaggtcagc
     2941 gcgctgcggt ccctctggct cgagtaccac ctccacctcg gcgccgtctc gcaatgcgac
     3001 acggcaccac gctgggccca gggcgatgac cggcccgccc tcccgcttct cgatgacgcc
     3061 gcgcatggga cggcctgcca cggatccggt taccggatgg cgaggtttgg cgccccatgg
     3121 ctcatccggg tcgaacggaa tcggcaccag cgtgcgcccc tgccgatcca cggcaaccgt
     3181 ggtccggaac ttccgttgcc cgctcacggg accagcctgc caccctccgc gcgtttccac
     3241 gcgggcacga gggtggggtc cgtctcgcac gacggcacgg tcacagcgcg ttcagcgggc
     3301 attcgacgcg aggatgagcc accacgcggg gaacgccatc atcgcgagcg cgatcggtat
     3361 cccgcccgcg acgaagagca ccatccgcat cgctgagtcc gccgagaaca ggaaggcgcc
     3421 tccgacgacc gccccgccca ccatgaaggc gagcaggacg ctgacactga gcgggaggaa
     3481 ccagcccggc acagccgacg cgccgaccgc tgccgtggcg gcgggccaca gccagatgaa
     3541 tccgacaaga gtcatcggcg ttgccaccac gaattgcacg ttgagcccga ctatgccggc
     3601 cagcatgagc gccgtcacga tggcacctac tgtaatgagg atgcaaagga ttccggtgag
     3661 gatccacagc agc
//
LOCUS       DATDBZ010000406         4338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_339148, whole genome shotgun sequence.
ACCESSION   DATDBZ010000406 DATDBZ010000000
VERSION     DATDBZ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339148"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VIX62_09690"
     CDS             <1..1018
                     /locus_tag="VIX62_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5860543.1"
                     /translation="EPIGVAGLIAPWNYPLMMAVWKIGPALASGNTIVLKPSEMTPLS
                     TIKLAELAAEIFPPGVFNVVTGDGLPVGDRLVRHPDVGIVSLTGDVNTGKLIHTNASD
                     TLKRVHLELGGKAPVVVFDDADLESVVEWISIAGYFNAGQDCTAACRVLVAEGAYDKV
                     LTDLVPAVEGLKVGTAFEDDVAVGSMISQEQLQRVEGFLSRAKDDGAEILTGGESLGT
                     PGFHFKPTVVANPSQDAEIVQREVFGPVVTVQRFTDEDQALSWANGTDYGLASSVWTR
                     DVGRAMRMSRRLQFGCVWVNTHIPLTPEMPHGGYKQSGHGKDMSVYSLEDYTNIKHVM
                     VSLD"
     gene            1163..2593
                     /locus_tag="VIX62_09695"
     CDS             1163..2593
                     /locus_tag="VIX62_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5860544.1"
                     /translation="MVETRPFLVNGEWRTGEGTFEVHSPYDGSIVAEIAVPTAADVEE
                     ASQVAHDTFEESKNLPIWARADALDHISKRLSETIEENANLIAAEGGKPLKWATVEAT
                     RAVSTFRWASEVIRHGTDEAMRLDTEQALGSRLGLLRRFPLGPVLGITPFNFPLNLVA
                     HKVAPSLAVGAPIVVKPASATPIGALRLAEFFAETDLPKGMYQVLPVSSKVADGMARD
                     DRFKKISFTGSSEIGWYLKGLDPKKRVTLELGGNAGVIVHSDADLDFAAQRIAFGGYY
                     QAGQSCISVQRVLVASEVYDDFAARLTKQVESLKVGDPMDPTVDVGPVIQAQEVERIQ
                     GWVDEAVSQGAQVLTGGTGEGPIFQPTLIADVKPEMKVCREEVFGPVVTISPYQTFDD
                     ALKSVNDSKYGLQAGVFTNDISRAMEAHRTLEVGGVIINDVSAFRADQMPYGGSKDSG
                     FGREGLRFAMEEMTEQRIMVLSHVPL"
     gene            2618..2743
                     /locus_tag="VIX62_09700"
     CDS             2618..2743
                     /locus_tag="VIX62_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860545.1"
                     /translation="MVFAYLGLAALIVLGIFTIVHLVRSWKVAGRRWGGGPGQGP"
     gene            2753..3043
                     /locus_tag="VIX62_09705"
     CDS             2753..3043
                     /locus_tag="VIX62_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860546.1"
                     /translation="MNQDRILYLALPFLVMLFVIGGFLLVWSRGRLDDRPWWGNPLLW
                     LTLCVVFVLLGVFVWPGLFGGMFLFLPFVWVWRPRRPPPVDPRTNGHARSDR"
     gene            complement(3051..3698)
                     /locus_tag="VIX62_09710"
     CDS             complement(3051..3698)
                     /locus_tag="VIX62_09710"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5860547.1"
                     /translation="MTFTTEDGVRLEGELRLPDEAPRASAVLCHPHPRHGGSKDHPIL
                     WALRNELAGTQELAVLGFNFRGNMGSEGTYGGGRDEVRDVRAAIERVRMEAPDLPSVV
                     CGWSFGANVALREAVDDERVAALVLIGIPLQPKDVTLPALPPPEELRRFRRPVLILAG
                     DNDEYCPAEEARVFSAEFVDGRLALLEGTNHFLWRREREAATIVGDFVDEVFGRT"
     gene            3728..>4338
                     /locus_tag="VIX62_09715"
     CDS             3728..>4338
                     /locus_tag="VIX62_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5860548.1"
                     /translation="MHDERQPSRVEGTAVALRDPLPWDRFAELVRLAESLEYRAVFLP
                     EIAGRDAFAALTALAGETSSLLLGTGIVPMTSRIPRLTAMGAATVNERSGGRAILGIG
                     TGPARKGALDALAAQVEQIRALIGGAPGSLLDEDLDPLSLRSPSAVPVWIAGLGPRSM
                     RLAGRIADGVLLNWCSPERVAQARLDIRAAAVGAGRDPEAVTVA"
BASE COUNT          689 a         1459 c         1501 g          689 t
ORIGIN      
        1 cgagccgatc ggggtcgcgg gcctgatcgc gccatggaac tacccgctga tgatggcggt
       61 ctggaagatc ggtcctgcgc tcgcctcggg caacacgatc gtgttgaagc cgtcggagat
      121 gacgccgctg tcgacgatca agctcgccga gctcgccgcc gagatcttcc cgcccggggt
      181 cttcaacgtc gtcaccggcg acgggttgcc cgtcggcgac cggttggtgc gccaccccga
      241 cgtcgggatc gtctcgctga ctggtgacgt gaacaccggc aagctgatcc acacgaacgc
      301 cagcgacacg ctgaagcgcg tccacctgga gctgggtggc aaggcgcccg tcgtggtctt
      361 cgacgacgcc gatctcgagt cggtcgtcga gtggatctcg atcgcgggct acttcaacgc
      421 cggacaggac tgcaccgccg cgtgccgcgt gctcgtggcc gaaggcgcct acgacaaggt
      481 gctcaccgac ctcgtcccgg cggtggaggg cctcaaggtc ggtaccgcgt tcgaggacga
      541 tgtcgcggtg ggctcgatga tcagccagga gcagctgcag cgggtcgaag ggttcctgtc
      601 ccgcgccaag gacgacggcg ccgagatcct caccggcggc gagtccctgg gcaccccggg
      661 cttccacttc aagccgacgg tcgtggccaa cccctcgcag gacgccgaga tcgtgcagcg
      721 tgaggtcttc gggccggtgg tgaccgtgca gcggttcacc gacgaggatc aggcgctctc
      781 ctgggcgaac gggaccgact acggcctcgc ctcgtccgtc tggacgcggg acgtgggtcg
      841 cgcgatgcgc atgtcccggc gcctgcagtt cggctgcgtg tgggtcaaca cccacatccc
      901 gctcacgcct gagatgcccc acggtgggta caagcagagc ggacacggca aggacatgtc
      961 cgtctattcg ctggaggact acaccaacat caagcacgtg atggtctcgc tcgactagcg
     1021 gccatcccga cacccgctca agcagcgaag cggtagcggg tcaaaagagc cccgctcaag
     1081 cagcgaagcg gtagcgggtc aaaagagccc cgctcaagca gcgaagcggt agcgggtcaa
     1141 agagagaaga ggagcacctc cgatggtcga gacgcgtccg ttcctggtga acggggagtg
     1201 gcgaaccggc gaaggcacct tcgaggtgca cagcccgtac gacggcagca tcgtcgccga
     1261 gatcgccgtt cccaccgccg ccgatgtcga ggaggcctct caggtcgccc acgacacctt
     1321 cgaggagtcg aagaacctgc cgatctgggc gcgcgccgac gcgctcgatc acatctccaa
     1381 gcggctgtcg gagacgatcg aggagaacgc gaacctgatc gcggccgaag gtggcaagcc
     1441 actgaagtgg gcgacggtcg aggccacccg tgcggtctcg acgttccggt gggcctccga
     1501 ggtgatccga cacggcaccg acgaagcgat gcgcctggac accgagcagg cgctgggttc
     1561 gcggctcggg ttgctccgtc gcttcccgct gggtccggtg ctcggcatca cacccttcaa
     1621 cttcccgttg aacctggtgg cccacaaggt ggcgccctcg ctcgcggtcg gagcgccgat
     1681 cgtggtgaag ccggccagcg cgacgccgat cggtgcgctg cgcctcgccg agttcttcgc
     1741 cgagaccgac ctgcccaagg gcatgtacca ggtgctgccg gtcagctcca aggtcgcgga
     1801 cggcatggca cgggacgacc ggttcaagaa gatctcgttc acgggatcgt cggagatcgg
     1861 ctggtacctc aagggcctgg acccgaagaa gcgcgtgacg ctcgagctcg gcggcaatgc
     1921 gggcgtcatc gtccacagcg acgcggactt ggacttcgcc gctcagcgca tcgcgttcgg
     1981 cggctactac caggcgggac agagctgcat cagcgtgcag cgggtgctcg tcgcctccga
     2041 ggtctatgac gacttcgcgg cacgcctcac caagcaggtg gagtcgttga aggtgggcga
     2101 cccgatggac ccgaccgtcg acgtcggtcc ggtgatccaa gcccaggaag ttgagcgcat
     2161 ccagggctgg gtcgacgagg ccgtcagcca gggcgcgcag gtgctcaccg gcggcaccgg
     2221 ggagggtccg atcttccagc ccacactgat cgccgacgtg aagcccgaga tgaaggtgtg
     2281 ccgcgaggag gtcttcggac ccgtcgtcac catcagcccc tatcagacgt tcgacgacgc
     2341 gctgaagtcg gtgaacgact cgaagtacgg cctgcaggcc ggggtcttca ccaacgacat
     2401 cagccgggcg atggaagccc accgcacgct cgaggtgggc ggcgtcatca tcaacgacgt
     2461 cagcgcgttc cgcgccgatc agatgccgta cggcgggtcg aaggattccg ggttcggccg
     2521 cgagggcctg cggttcgcga tggaggagat gaccgagcag cgcatcatgg tgctctcaca
     2581 cgtgccgctc taagcacgcc gtgacaggct ggtccccatg gtcttcgcct acctcgggct
     2641 cgcggcgctc atcgtgctgg gcatcttcac gatcgtgcat ctggtcagat cgtggaaggt
     2701 ggccggccgc cgttggggcg gtggtccggg tcaaggaccc tagcgtggct cgatgaacca
     2761 ggatcggatc ctttatctcg cgttgccgtt cctggtgatg ctgttcgtga tcggcgggtt
     2821 cctgctggtc tggagccgtg gccggctgga tgaccgtccg tggtggggga acccgctgct
     2881 ctggctcacg ctgtgcgtcg tgttcgtgct gctgggcgtc ttcgtgtggc cggggctgtt
     2941 cggcgggatg ttcctgttcc taccgttcgt gtgggtgtgg cggcctcgcc ggccgccgcc
     3001 ggtggaccct cggacgaacg ggcatgcacg ctcggatcgc tgacgccccg tcaggttcgg
     3061 ccgaacacct cgtccacgaa atccccgacg atcgtcgccg cttcacgctc ccggcgccag
     3121 aggaaatggt tggtaccttc gagcagcgcg aggcggccgt cgacgaactc ggcggagaag
     3181 actcgcgctt cctcggccgg gcagtactcg tcgttgtcgc ccgcgaggat cagcaccggc
     3241 cggcggaacc ggcggagctc ttccggtggt ggaagggccg gcagggtcac atccttcggt
     3301 tgcaacggga tgccgatcag caccagggcg gcgacgcgtt catcgtcgac cgcttcccgc
     3361 agcgcgacgt tggcgccgaa cgaccaaccg cacaccacgc tgggcaggtc aggggcctcc
     3421 atgcgcacgc gctcgatcgc ggcgcgcaca tcacggacct cgtcccggcc gccgccgtag
     3481 gtgccttccg atcccatgtt cccccggaag ttgaagccca acacggcgag ctcctgggtc
     3541 cccgcgagtt cgttccgcag cgcccacagg atgggatggt ccttgctgcc accgtgccgt
     3601 ggatggggat gacacagcac ggccgaggcg cgaggcgcct catcgggcag ccgcagctcg
     3661 ccctcgagcc ggacaccgtc ctcggtcgtg aacgtcatgc cttccacgac ctgcgacgat
     3721 ggcacccgtg cacgacgaac gacaaccgag ccgtgtggag gggaccgcgg tggccctgcg
     3781 cgatccgctc ccgtgggacc ggttcgccga gctcgttcgc cttgccgagt cgctcgagta
     3841 ccgggcggtg ttcctgccgg agatcgcggg tcgtgacgcg ttcgccgcgc tgaccgccct
     3901 cgcgggggag acgtcgtcgc tgctgctcgg aaccggcatc gtcccgatga cctcgcggat
     3961 ccccaggctc accgcgatgg gcgccgccac cgtcaacgaa cgcagcgggg gacgagcgat
     4021 cctgggcatc ggaaccggcc ccgcacgcaa gggcgccctc gatgccctgg cggcgcaagt
     4081 tgagcagatc cgtgccctga tcggcggcgc accggggtcg ctcctcgacg aggacctcga
     4141 cccgctctcg ctgcggtccc cgtccgcggt gcccgtgtgg atcgcggggc tggggccacg
     4201 atcgatgcgg ctcgccggac ggatcgccga cggcgtgctg ctcaactggt gctcacccga
     4261 acgcgtcgcg caggcccggc tcgacatccg cgcggccgcc gtcggcgccg gccgcgaccc
     4321 cgaggccgtg acggtcgc
//
LOCUS       DATDBZ010000407         2193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1351762, whole genome shotgun sequence.
ACCESSION   DATDBZ010000407 DATDBZ010000000
VERSION     DATDBZ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351762"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VIX62_09720"
     CDS             <1..829
                     /locus_tag="VIX62_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860549.1"
                     /translation="QAMREIRGEGHEGADQDGAPTAVRQPVSIEDRRAASPFMKELSM
                     DAKKSLTRILGITQILKHKKDAKEQAALMRQLTAHTRRLDRTVADLADADRLSQGAIE
                     LTVRRTDLEPMIQRVVEESGLEADHELRVHAERLVVAVDPQRTEQIISALLRTSGDRT
                     PPKKAITIRLIPSDGGALIVVEDPEPSSDVSINPVVERFAEVQGGWAKVESQESGGSA
                     FSVFLPDGAGIGDPVDGPASGREVKVVVDEPVETWEPHDEKALVDELHRLSSAETDQ"
     gene            complement(838..2022)
                     /locus_tag="VIX62_09725"
     CDS             complement(838..2022)
                     /locus_tag="VIX62_09725"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5860550.1"
                     /translation="MTTRSVILAGARTPIGKFQGSLAGFRAVELGAIAIAEALTRSGV
                     APEQVEYTIMGHVLQAGQGQITARQAAIGAGIPKESPALTVNKVCLSGMSAIAIADQM
                     IRAGDADVVVAGGMESMTNAPFVLPKARDGSRLGDSPMIDSLIHDGLWCAFDDQHMGA
                     GTDAISAELGVGRADQDRWAARSHVRAHDAWEQGRLDDEVVAVEVPQRNGDPIVFARD
                     EGIRPDSSPERLARLRPAFTSEGTVTAGNASQISDGAAAVVVVSEDIAERMHGEPLAG
                     IVAYGMSADRYPSLHTVPALALQKAAERGGVDAADLGLLEVNEAFAAVPVHTARMLGA
                     DEELVNVNGGAVALGHPIGASGARIVLTLAMEMRRRGVALGGAAICGGGGQGDALILR
                     RP"
BASE COUNT          377 a          766 c          717 g          333 t
ORIGIN      
        1 gcaggcgatg cgtgagatcc ggggagaggg tcacgagggc gccgaccagg acggcgcacc
       61 gaccgcagtg cgtcagcccg tgtccatcga ggatcgccgt gccgcctctc cgttcatgaa
      121 ggaactgtcg atggacgcga agaagtcgct cacccgcatc ctgggcatca cgcagatcct
      181 gaagcacaag aaggacgcaa aggaacaggc tgcgctcatg cgccagctca cggcccatac
      241 gcgccggctg gaccgaaccg tcgccgacct cgccgatgcc gaccgtcttt cccaaggcgc
      301 gatcgagctg acggtccgtc gtaccgacct ggaacccatg atccagcgcg tcgtcgagga
      361 atcgggcctc gaggccgacc acgagcttcg ggtgcacgcc gagcgattgg tcgtggcggt
      421 cgatccgcag cgcaccgaac agatcatctc cgccctcctt cggacctccg gcgaccggac
      481 gcccccgaag aaggcgatca cgatccgctt gatcccgagc gacggcggtg ccttgatcgt
      541 ggtcgaggac ccggagccgt cgtcggacgt gtccatcaac cccgtggtcg aacggttcgc
      601 cgaggtgcag ggtggatggg ccaaggtcga gagccaagaa tcgggcggat cagccttctc
      661 ggtgttcctc cccgatgggg ccggcatcgg cgatcctgtc gatgggccag cgtcgggccg
      721 agaggtcaag gtcgtggtcg acgagcccgt cgagacctgg gaaccgcacg acgagaaggc
      781 gctggtcgac gaactccatc gtctgtcgag cgccgagacc gaccagtagg agcggcgtca
      841 ggggcgacgg aggatcaggg cgtcgccctg gccaccaccg ccacagatcg ccgcgccacc
      901 gagcgccacg ccgcgccgcc gcatctccat cgccagcgtg aggacgatgc gggcaccgct
      961 ggccccgatc gggtgtccca gggcgaccgc accgccgttc acgttcacca gttcctcatc
     1021 ggcgccgagc atccttgctg tgtgcaccgg caccgcagcg aaggcctcgt tcacttcgag
     1081 gagcccgagg tcggcggcgt cgacaccgcc gcgttcggca gccttctgca gcgccaacgc
     1141 gggcacggtg tgcaacgacg ggtagcggtc ggccgacatc ccgtaggcaa cgatcccagc
     1201 caggggctca ccgtgcatgc gttcggcaat gtcctcgctg acgaccacga ccgcggccgc
     1261 accatcggag atctgcgatg cgttgcccgc cgtcaccgtc ccctccgaag tgaaggccgg
     1321 ccggaggcgc gcgagtcgct cgggcgaact gtcgggacgg atgccttcat cgcgcgcgaa
     1381 cacgatgggg tccccgttcc gctgaggcac ctcgacggcc acgacctcgt cgtcgagccg
     1441 accctgttcc cacgcgtcat gggctcgcac gtgtgaacgc gccgcccacc gatcttggtc
     1501 agcccggccg acaccgagtt cggcgctgat ggcatcggtg ccggcaccca tgtgctgatc
     1561 gtcgaacgcg caccacagac cgtcgtggat caacgaatcg atcatgggtg aatcgcccaa
     1621 tcgcgatcca tcgcgagcct tgggcagcac gaacggagcg ttcgtcatgg actccatgcc
     1681 gccggcgacg accacgtctg cgtcgcccgc acggatcatc tggtccgcga tcgcgatcgc
     1741 ggacatgccc gacagacaca ccttgttgac cgtgagcgcg ggagactcct tcgggatccc
     1801 ggcgccgatg gcagcctgac gagcggtgat ctgtccctgg cccgcctgca ggacatggcc
     1861 catgatcgtg tactcgacct gctcgggcgc cacacccgag cgtgtgagcg cctctgcgat
     1921 cgcgatggcg cccagctcga cggcgcggaa ccctgccaac gagccttgga acttgccgat
     1981 gggcgtccga gccccagcca ggatcaccga ccgtgtcgtc atgtcggcag tctatcgccg
     2041 ggcccgcgaa cccttgaggc gggagcctac gagcggttgg cgagcgcctt ctcgatgaca
     2101 tcgaggatgt catcgcggac cagttcatct ctggcgatca gtgcgtcgcg aagtgcgcca
     2161 tgcacgtcac ggttctcctc cagcacctcg cgg
//
LOCUS       DATDBZ010000408         3916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1352486, whole genome shotgun sequence.
ACCESSION   DATDBZ010000408 DATDBZ010000000
VERSION     DATDBZ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352486"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..464
                     /locus_tag="VIX62_09730"
     CDS             243..464
                     /locus_tag="VIX62_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860551.1"
                     /translation="MMTPFGSELAHERAATVRRQRGRGLSMILVVIGGLAVLGAAAWF
                     WGEDSRGCFDPRDDGGHDPFLPMDRRTSR"
     gene            483..776
                     /locus_tag="VIX62_09735"
     CDS             483..776
                     /locus_tag="VIX62_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860552.1"
                     /translation="MLRFTDQAAQAMVRSDAAARRFNPDARVRLVPDPARGVRAELVD
                     GPEPGDTLQQIQDGPQVFVASSLSGSVDAGEHDLLVLTPGPAITTEAAGSGPV"
     gene            793..1665
                     /locus_tag="VIX62_09740"
     CDS             793..1665
                     /locus_tag="VIX62_09740"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY5860553.1"
                     /translation="MTDGGGPELASLAALGGGPVMVIGGAEDHVRDKVILSRFVKLAG
                     GADGHVVVISTASSLGDQATQVYRDLFMKLGVGTVTGVRPEERADADDPAMAVMLREA
                     TGVYLTGGNQSRLTQVVAGTKLADALFLAHDRGAVLAGTSAGASAMASHMVAFGNAGP
                     TPKNRMVQLSAGLGILSGLVIDQHFGERGRIGRLMALVAQSPSLLGVGVDEDTCAMVF
                     PDHTLTVIGKGAITIVDGAHVKTDAYRGKGYKPLMVSGAVLHSLPAGYWFDLRGRRLL
                     AAEGALEDEREASE"
     gene            1758..>3916
                     /gene="cphA"
                     /locus_tag="VIX62_09745"
     CDS             1758..>3916
                     /gene="cphA"
                     /locus_tag="VIX62_09745"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY5860554.1"
                     /translation="MKVVATQVFRGPNYWSYEPCIRMLVDLGSLEDWPSNTIPGFNRK
                     LLKILPGVGEHSCSLGKTGGFKERLEDGTWLGHVAEHVALELQRESGAHISRGKTRSA
                     GSAGQYNVIYGYAEERVGIEAGSLAVRLVNHLVKAEPGFDFDGQLERLILLAERRQFG
                     PSTQAILDEALSRDIPWIRLNEYSLVQLGQGTYQQRIRATMTSQTSALAVDIAGDKKM
                     TNGLLAAAGLPVPKSEVVRAEDDAVAAALRIGFPVVTKPLDGNHGRGVGLELRTERAV
                     RTGFKRALKESRHGQVVVESFVTGSDYRVLVIGGRMVAIAERVPAHVTGDGRRTIREL
                     VEKANQDPRRGIGHEKVLTRIKVDEAAADLVKKQGFALEEVPPKGEQVLLAATGNMST
                     GGISIDRTWDAHEDNVEIAEEAARVVGLDVAGIDFLATDISTPVRETGGAIVEVNAAP
                     GFRMHTHPTEGEPQYVARPVIDLLFPAGSPSRIPIVAVTGSNGKTTTVRMIAHVMKNM
                     GRKVGLTSTDGIYIDGRLTKRVDASGPRSAQTVLQNPRVDFAVFEVARGGILREGLGY
                     GRNDVAVVLNVTGDHLGLREIDTIEQLAAVKRVIVEAVPRDGYAVLNADDPLVLDMRK
                     HCSGSVILFTMNDQHELIERWVRRGRKAVVLRRESGGEQIVLRDGRRTMPVGYTHQFP
                     ATFDGRARMNVQNLLAAVAACHVSGAHLHDIRHGLRT"
BASE COUNT          662 a         1253 c         1366 g          635 t
ORIGIN      
        1 ttccagggtg gaccgggtgg gtggcaggct cgcatcgcga ccgtcctcgt gttcgtgcaa
       61 cgacatgaaa ctccggacgt cctcgaggcc gtcgggcgcg ggggcgttca catggtcctc
      121 gtggctcgac tcgatcattc cggcagctta ctccttgaaa cttgtgaaga ccagttgact
      181 ggttacacct aaccagtata agacaggtta tcggttacac actccgagga ggaggcaccg
      241 cgatgatgac cccgttcgga tcggaactgg cccacgagag agccgcgacc gtcaggcgtc
      301 agcgcggccg aggcctcagc atgatcttgg tggtgatcgg tggactcgcc gtgctgggcg
      361 cagccgcgtg gttctggggc gaagactccc gagggtgctt cgatcccagg gacgacggcg
      421 gacacgaccc gttcctgccc atggatcggc gcacctctcg ttgacgccgt agcatcttct
      481 ccatgcttcg gttcaccgat caggcggcgc aggcgatggt gcgcagcgac gccgccgcac
      541 gacgattcaa ccccgatgca cgggttcgcc ttgtaccgga tccagctcgg ggtgttcgtg
      601 ctgaactggt cgacggacca gagccggggg acaccctcca gcagatccag gacggaccgc
      661 aggtgttcgt ggcatcgtcc ctgagcggct cggtcgatgc gggtgaacac gatctcctcg
      721 tcctcacgcc cgggcccgcg atcaccaccg aggctgcggg ttccggccct gtgtgacggc
      781 tacacttcgg acatgacgga tgggggcggg cccgagctcg catcgctcgc cgccctcgga
      841 ggcggcccgg tcatggtgat cggtggcgca gaggaccacg ttcgcgacaa ggtgatcttg
      901 tcccggttcg tgaagctcgc gggcggcgcc gacgggcacg tcgtggtgat ctccacggca
      961 tcgtctctcg gcgatcaagc gacccaggtg tatcgagacc tgttcatgaa gttgggtgtg
     1021 gggaccgtga cgggcgtgcg acccgaagag cgtgccgacg cggacgatcc ggcgatggcc
     1081 gtgatgctgc gtgaggcgac aggggtctac ctcaccggtg gcaaccagtc gcggctgacc
     1141 caggtggtcg cgggcaccaa gttggccgac gcgctcttcc tggcccacga tcgtggggcc
     1201 gtcctcgccg gtaccagtgc gggcgcgagc gccatggcga gtcacatggt ggcgttcggc
     1261 aacgctgggc ccacaccgaa gaaccgcatg gtgcagctgt cggccggtct cggcatcttg
     1321 tccggactgg tcatcgacca gcatttcggc gagcggggac gcatcggccg gttgatggcc
     1381 ctggtggcgc agtcgccgtc gttgctcggc gtgggcgtcg acgaagacac ctgcgcgatg
     1441 gtcttccctg atcacacact caccgtgatc ggcaaggggg ccatcacgat cgtcgatggt
     1501 gctcacgtca agacggacgc atatcgcggg aaggggtata aaccgctcat ggtgtcgggc
     1561 gcggtgttgc actcactgcc ggcgggctac tggttcgacc ttcgcggtcg gcggttgctc
     1621 gcggctgagg gcgcgctcga ggatgagcgg gaggcctcgg agtgacggcg aaggccgcga
     1681 aaccgaccac atcgcgagcg tcccgccagc gcgccaaacg cgagccgacc tcgaagaaac
     1741 caccgctgaa gcccgatctg aaggtggtcg cgacccaggt cttccggggt cccaactact
     1801 ggtcgtacga accctgcatc cgaatgctcg tcgacctcgg ttcgttggag gactggccgt
     1861 ccaacacgat ccccggtttc aatcgcaagc tgctgaagat ccttcccggt gtcggagagc
     1921 actcctgttc gctcggcaag accggtgggt tcaaggagcg gctcgaggac ggcacctggt
     1981 tgggccacgt cgccgaacac gtcgccctcg agctccaacg cgagtcgggt gcccacatct
     2041 cgaggggcaa gacgcgcagc gctggttcgg cgggtcagta caacgtgatc tacggctacg
     2101 cggaggagcg tgtcgggatc gaggccggct cgctcgccgt ccgcctggtg aaccacctcg
     2161 tgaaggcgga gccagggttc gacttcgacg gacagctcga acgcctgatc ttgctcgccg
     2221 agcgccggca gttcggtccg tcgacccagg cgatcctcga tgaggcgctg agtcgcgaca
     2281 tcccatggat ccggttgaac gagtactcgt tggtgcagtt ggggcagggg acctatcagc
     2341 aacggatccg cgccacgatg acttcgcaga ccagcgcact cgccgtcgac atcgcgggcg
     2401 acaagaagat gaccaatgga ctcctcgccg ccgccgggtt gcccgttccc aagagcgagg
     2461 tcgtgcgagc cgaggacgat gccgtggctg ccgcgttgcg catcgggttc ccggtcgtca
     2521 ccaaaccgct ggacggtaac cacggccgcg gcgtggggct ggaactgcgc accgaacggg
     2581 ccgtgcgaac cgggttcaag cgggccttga aagagtcgcg tcatggacag gtcgtcgtcg
     2641 agtcgttcgt gaccgggagc gactaccgcg tcctggtgat cggcgggcgc atggtggcga
     2701 tcgcagagcg ggtgccggcg cacgtgaccg gcgacggcag gcgcacgatc cgtgagttgg
     2761 tggagaaagc caaccaagat ccgcggcgag ggatcggtca cgagaaggtg ctcacccgca
     2821 tcaaggtcga tgaggccgcc gcggatctgg tgaagaagca agggttcgcg ctggaagaag
     2881 tgcctccgaa gggcgagcag gtactgctgg ccgccaccgg caacatgtcc accggcggca
     2941 tctcgatcga ccgcacctgg gacgcacacg aggacaacgt ggagatcgct gaggaagccg
     3001 cccgggtcgt cggcctggat gtcgccggca tcgacttcct ggccaccgac atctccacgc
     3061 cggtccggga aaccggcggc gcgatcgtcg aggtgaacgc ggcgcccggg ttccgcatgc
     3121 acacgcaccc caccgaaggg gagccgcagt acgtggcgcg ccctgtgatc gatctgctct
     3181 tccctgccgg gtcgccctcg cgcatcccga tcgtggccgt gaccggctcc aacggcaaga
     3241 ccacgacggt tcgcatgatc gcgcacgtga tgaagaacat gggccgcaag gtcggcctca
     3301 cgtcgaccga cggcatctac atcgacggcc gcctcacgaa gcgcgtcgac gcgagcggcc
     3361 cccgctcggc gcagaccgtg ctgcagaacc cccgagtgga cttcgcggtg ttcgaggtcg
     3421 cgcgcggtgg catcctgcgg gagggcctcg gctacggccg caacgacgtg gcggtggtgc
     3481 tgaacgtgac gggcgatcac ctcggcctgc gcgagatcga cacgatcgag cagctcgcgg
     3541 cggtcaagcg cgtgatcgtg gaagccgtgc cgcgcgacgg ctacgccgtg ctgaacgccg
     3601 acgacccgct ggtgctcgac atgcgcaagc actgctcggg cagcgtgatc ctgttcacga
     3661 tgaacgacca gcacgagctg atcgagcggt gggtgcgccg aggccgcaag gccgtcgtgc
     3721 tgcggcgaga gtccggtggc gagcagatcg tgctgcgcga tggtcgccgc acgatgccgg
     3781 tgggctacac gcaccagttc ccggccacgt tcgacggccg ggcgcgcatg aacgtgcaga
     3841 acctgctcgc ggcggtggcg gcgtgccacg tctcgggcgc ccacctgcac gacatccgcc
     3901 acggtttgcg cacgtt
//
LOCUS       DATDBZ010000409         3356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1978438, whole genome shotgun sequence.
ACCESSION   DATDBZ010000409 DATDBZ010000000
VERSION     DATDBZ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978438"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VIX62_09750"
     CDS             <1..977
                     /locus_tag="VIX62_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY5860555.1"
                     /translation="PDGSVPFRPDMLETRKYIARTAVKAFDRRTDVDPSITFTDVDPS
                     QSFYRWANIAVQRGWMEPSADGRFNPEGVVTTASLHDILIDVLGLRRIARQLDALQTR
                     GGVRFPTPRHFGALMLGLRLGLRYNNSNESMDVGPRTPLTRAQVAYSLYKASTLPSWV
                     VPWLRDQYDGIVLPNMDDDRQAIVRWGVRYVGYPYVWGGEWGIDSSQPVPGFDCSGLV
                     WWAMRQNDGGSWMVHPPRPYAGWTLPQRTSADMARFGNLKYDALRPGDLMFYDGNDDG
                     TVDHVDVYVGNGYALDSSSSPGGVTIMWVGSGWYRDHFVHGRRILPTG"
     gene            complement(986..1384)
                     /locus_tag="VIX62_09755"
     CDS             complement(986..1384)
                     /locus_tag="VIX62_09755"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY5860556.1"
                     /translation="MHEVRLRIRWRDIDNYGHVNNAVYLNYLEECRDRMVEDLFGPEA
                     WDFVLAHVGIDFRNELTQADGEVVVRCEVVGYGRSSLRTRERIEKTDGTLAAEAESVI
                     VPRSPHEPSSRALTDGERAVLQREVDASNA"
     gene            complement(2323..>3356)
                     /locus_tag="VIX62_09760"
     CDS             complement(2323..>3356)
                     /locus_tag="VIX62_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5860557.1"
                     /translation="GDPDEHVGTFVAEALGGLRQARAEPDVAILLAVGGAQFVMVGML
                     DVFFALLAIDVLGSGESGAGVLAAGFGLGLLVGAAGAVGLVGVRRLAPAVLVALLGSG
                     VSLAALALAASVPLAVLVLALCGATIGLFAVSSRTLLQRRADDEVLARVFGLEEALQM
                     VGLAIGAAAAPLLVASFGARGALAAAGGLLALVAVVASPRVRRIDAKAEGPGQDFDLL
                     RRVTIFAPLPQYVKEQLARHLITVLARSGDVVIREGDTGDRVYVIRRGRVAVTKAGRL
                     VAELGPGDYVGEIALLRDVPRTATVTAVDDTELLALERDVFLGAVTGAHPNRGHIDAE
                     MDRRLADES"
BASE COUNT          515 a         1193 c         1115 g          533 t
ORIGIN      
        1 gacccgacgg ctcggtgccc ttccgccccg acatgctcga gacgcgcaag tacatcgcga
       61 ggaccgccgt gaaggcattc gaccggcgaa cggacgtcga cccgtcgatc acgttcaccg
      121 acgtcgatcc gagccagagc ttctaccggt gggccaacat cgccgtgcaa cgaggatgga
      181 tggaaccatc ggccgacggt cgcttcaacc ccgagggcgt ggtcaccacc gcgtcgctgc
      241 acgacatcct gatcgacgtg ctcggcctgc gccgcatcgc gcgccagctc gacgcgctgc
      301 agacccgcgg tggggtgcga ttcccgaccc cgcgccactt cggtgcgctg atgctcgggc
      361 tgcgcttggg cctgcgatac aacaacagca acgagtcgat ggacgtcggc ccacgcacgc
      421 cactgacgcg cgctcaggtc gcgtactcgc tctacaaggc cagcactctt ccctcgtggg
      481 tggtgccctg gctgcgtgat cagtacgacg gcatcgtgtt gcccaacatg gacgacgacc
      541 gtcaggcgat cgtgcgctgg ggggtgcggt acgtggggta tccctacgtg tggggcggcg
      601 agtggggcat cgacagcagc cagcccgtgc ccgggttcga ctgttccggt ctcgtgtggt
      661 gggcgatgcg ccagaacgac ggaggctcgt ggatggtgca cccgcctcgc ccgtacgcag
      721 ggtggacctt gccgcaacgc acctcggctg acatggcccg gttcggcaac ctcaagtacg
      781 acgccctgcg ccccggtgac ctgatgttct acgacggcaa cgacgacggc accgtcgacc
      841 acgtcgacgt ctacgtcggc aatggctacg cgctcgactc gtcgagctcc ccgggcgggg
      901 tcaccatcat gtgggtcggc agcggctggt accgcgacca cttcgtgcac ggtcgtcgca
      961 tcctgccaac gggctgacga tcccttcaag cgttcgacgc atcgacctcg cgctgcagca
     1021 cggctcgttc tccatcggtg agggccctgc tggacggctc gtgcggcgac ctcggcacga
     1081 tcacggactc cgcctcggcc gccagcgtgc cgtcggtctt ctcgatacgc tctcgcgtgc
     1141 ggaggctcga gcgtccgtag ccgacgacct cgcatcgcac cacgacctca ccgtcggcct
     1201 gcgtgagttc gttgcggaag tcgatgccga cgtgcgctaa cacgaagtcc cacgcttcag
     1261 ggccgaagag gtcttcgacc atgcggtcgc ggcactcctc gaggtagttc aggtagacgg
     1321 cgttgttcac atggccgtag ttgtcgatgt cgcgccaccg gatccgcaac cgcacctcat
     1381 gcatggtgga caagggtacg ggagcgggcg tcacccgttc atcacggctt gccggtgtgc
     1441 catgctccgg aagcgatgga ccacctttcg cgaccgcctc gatcgatgtc ggccaaggtg
     1501 gtactcctga tcgtcggcat aggggcgtgg tacctcgcgg tcctgccctt cctggacgct
     1561 cggtggtcgc cgccacgatg cgggaacccc tgcgcggggc cgtcgacttg gcctgcctcg
     1621 atcatcgtcg tgggggcgat cgcgccgttg atcctgctgt tcgtcctcac cgccggctcc
     1681 cggcggacga tgttctcggc gtggcgaccg cgaccgtctc catcgtggct ttcgcgatca
     1741 cgctgtcgtt cgagagccgg gcatgcccga acatctacgc gaacgactgc cacggtcgcg
     1801 gaacgctcgc cttgctccag tggatgacgt acgtcggccc cgtgctcatc gcgtcgttcg
     1861 cccttggccc gatccatggc cgctcgtgga cctcgtatct ggcgttaccc ttcgcgggac
     1921 tcatcggctt gggcatcgca gctgcgccgt ctgcgatcgg ggacgcgaac atcgacgggg
     1981 acgtgctgct ggcgtacatg atcggatggc cactcttcct ggtgccgctc atcatcgccg
     2041 cggtcgccgg ctcgaactcc gatgccgcac gctccgaaga tcgacggcga gggcgccggc
     2101 ctgccctctt ccgagtcgcc gggatcgagg tagttggaag cagccgttcg gctgatggac
     2161 gttgtccctc ggaggcaggc ccgaggatgg gagaaggccc cgggcggggg gcccgaggcc
     2221 ttctctgtcg gagagagggg ctccacccct acttcggttc atgccgcggt gtggttcggt
     2281 cgacccgtgt tgcggttctg tgacggctga tcgctcccac cctcagctct cgtcggccag
     2341 ccgccggtcc atctcggcgt cgatgtggcc gcggttcggg tgtgcgcccg tcacggcgcc
     2401 gaggaagacg tctcgttcga gggcgagtag ttcggtgtcg tccacagcgg tcaccgtcgc
     2461 ggttcggggc acgtcgcgca gcaaggcgat ctcccccacg taatcgcccg ggcccagctc
     2521 ggcgacgagg cgccctgcct tcgtgacagc gacacgtccc cgtcggatca cgtacacccg
     2581 gtcccccgta tcgccttcgc ggatcaccac atcgccggac ctcgcgagca ccgtgatcag
     2641 gtgacgcgcc agctgttcct tcacgtattg cgggagcggc gcgaagatcg tgacacgacg
     2701 cagcaggtcg aagtcctgcc caggtccttc ggccttcgca tcgatgcgac gcacgcgagg
     2761 ggaggctacg accgccacga gggccagcaa cccgcccgcc gcggccaggg caccacgggc
     2821 gccgaaggaa gcgacgagga ggggagccgc cgccgccccg atggcgaggc cgaccatctg
     2881 gagggcttcc tcgaggccga acacccgagc cagcacctcg tcatcggccc gccgctgcag
     2941 cagggtgcgg ctcgagaccg cgaacaggcc gatcgtcgcc ccgcagagcg cgagcaccag
     3001 caccgcgagt ggcaccgagg ccgcgagcgc gagcgccgcg agcgagacgc cggagccgag
     3061 cagcgccacc agcaccgcag gcgccaagcg gcgcacgccc acgagcccca cggcaccggc
     3121 cgccccgacc agcaggccga ggccgaagcc cgcggcgagg acaccggcgc cgctctcgcc
     3181 cgacccgagc acgtcgatcg cgagcagcgc gaagaacacg tcgagcatgc cgaccatgac
     3241 gaactgggcg ccaccaacgg cgagcaggat cgccacgtcg ggctccgctc gcgcctgccg
     3301 cagcccgccg agcgcctcgg cgacgaacgt gcccacgtgc tcatcggggt cgccca
//
LOCUS       DATDBZ010000410         5480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2190449, whole genome shotgun sequence.
ACCESSION   DATDBZ010000410 DATDBZ010000000
VERSION     DATDBZ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190449"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VIX62_09765"
     CDS             complement(<1..489)
                     /locus_tag="VIX62_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY5860558.1"
                     /translation="MTTDVEERFRGPLVLDGGMATELERGGADLRDALWSARLLKDDP
                     GSIRRVHEAFFDAGADVAISASYQASFSGFADRGIEAEEAASLMRLSVALAREAAAAD
                     GSDRLVAASVGPFGATLADGSEYRGDYGLSAGALAMFHRPRFETLLEAEPDLLAIETI
                     PSI"
     gene            545..1645
                     /locus_tag="VIX62_09770"
     CDS             545..1645
                     /locus_tag="VIX62_09770"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5860559.1"
                     /translation="MRERLQALADRDVERWPQMPGRLIHVLAPEYGLDLEVAAGVADR
                     ASGEPLTPGSRFRIASVTKPFVGASALRLVETGRLDLDASLDVLLAEETTEVLREGGY
                     DTARITLRHLMTHTSGIYDFAASAYDPSIDDGFDAAIAADPTHRWTREEQLRFAIDHG
                     EPYGPPGAVYCYSDTNANLVGELLERETGTTMGAAIRDLVGYDRLGMHHTYQESIEPE
                     PADLPPWSHQYERDIDVLTIDPSVDLYGGGGLVSTCADLAKFFRALLLRGEVFDSPAT
                     LVTMATRLQGVPASERLATGDDPDDAGMFIFRASVGGLRWWGHDGWWGTTVYACPELE
                     LTVVAGHQQPYMPKEFDRMAVFADVMELVLAH"
     gene            1680..2858
                     /locus_tag="VIX62_09775"
     CDS             1680..2858
                     /locus_tag="VIX62_09775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5860560.1"
                     /translation="MSTYPMSEEDRAIQERARGFIDEDLIPWEQYAEEHDGEIPEAER
                     GKHHDKAIELGLFAMNMPVELGGTGMTTFQQVLVSEQLGRVTNALGWCVHTPPAWAPA
                     VVSEHQMDTWIKPTIRGEMHECYAITEEGAGSDVDAIEATARRDGDDYVLNGVKMHVT
                     SFNHAAYCFFQAKIVGGEHEGEHAMFFVDVDAPGVRVVRTPAYTHTYADHHPLVAFED
                     VRVPAANLVGAEGDGMAFTYEWFRYERLMIAARCCGAADRLITEATAFAKERVQFGEP
                     IFAFQAIQHMLADSLTELWAARLMTYELAKNIDRGVDVKLQHAQCSMAKLYASEMAGR
                     VADRAVQIFGGRGYMRENVAERFWREVRVDRIWEGTSEVQRSIIADQLGKRGVSPLIG
                     "
     gene            3021..4514
                     /locus_tag="VIX62_09780"
     CDS             3021..4514
                     /locus_tag="VIX62_09780"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5860561.1"
                     /translation="MERLSHWIGGKLVSGESGRTGAVFNPATGEQTHEVDFADVAEVD
                     AAIEVAREAFVSWRATSLSKRADILFRIRESIHEHRADIGRIITSQHGKVLSDAMGEV
                     ARGLENVEFATGIPHLLKGGFTEQASTGVDVYSIRQPLGVVAGITPFNFPAMVPMWMF
                     ANAIACGNTFVLKPSEKDPGASLYVAELLKDAGLPDGVLNVVQGDKVVVDRLLEHPDV
                     EAVSFVGSTPIAKYIYATGTANGKRVQALGGAKNHMVVLPDADIDMAADAAVSAAYGS
                     AGERCMAISVAVAVGDAGDRLVEAIKERLPLVKIGNGLEPESEMGPLVTREHRDKVAG
                     YLDSAKAQGATIVEDGREHGLYRESDGFFLGVSLIDHVTTDMDAYKDEIFGPVLEVMR
                     VDSYDEALGLVNDNPYGNGTAIFTRDGGAARAFQFDAQAGMVGVNVPIPVPVGYYSFG
                     GWKDSLFGDSHMYGPEGVNFYTKGKVVTARWPDPGTSSVDLGFPRTR"
     gene            4525..4977
                     /locus_tag="VIX62_09785"
     CDS             4525..4977
                     /locus_tag="VIX62_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5860562.1"
                     /translation="MTDSAQGFLEREAASWDAFEAQVDRVTAEQREVPGVVEGWSLKD
                     VVWHCAYWARFSADHLVMEGDGPHTDPFATLTAEEADRENAAIAEASSAMSWDDVWEG
                     AADARASVRSVIATPGLSSEQIDFAAEESWVHYDEHAEHVRAFADALG"
     gene            complement(4981..>5480)
                     /locus_tag="VIX62_09790"
     CDS             complement(4981..>5480)
                     /locus_tag="VIX62_09790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860563.1"
                     /translation="RWPLIAGDDIGERIERAFGSIYLGIVDLGLIWEAGDGDGFAVWI
                     PPGMSDQMFESDTAARDLVAPLTEDHAARYDVLWSWIEAQTPADAWYLDVLGVDPTKQ
                     GMGVGSALVRFGLERAARDGADAFLETAVEANVAYYERFGFRTVFDGRAADDAPRIWF
                     MRTGG"
BASE COUNT          902 a         1868 c         1879 g          831 t
ORIGIN      
        1 gatcgagggg atcgtctcga ttgccaagag gtcgggttcg gcctcgagga gcgtctcgaa
       61 ccgcggtcgg tggaacatcg cgagcgcccc cgcggacagg ccgtagtcac cgcgatactc
      121 cgagccgtcg gccagcgtgg cgccgaacgg tcccaccgag gccgccacca gtcgatcgga
      181 tccgtcggcc gcggcggctt cgcgagcgag cgccacgctc aggcgcatca gcgacgcggc
      241 ctcctcggcc tcgatgccgc ggtcggcgaa cccggagaac gaggcctggt agctcgccga
      301 gatcgcgacg tcggccccgg cgtcgaagaa cgcttcgtgc acccgacgga tcgacccggg
      361 atcgtccttg aggagccggg ccgaccagag ggcgtcgcgc aggtcggcgc cgccacgttc
      421 gagttcggtg gccatcccgc cgtcgaggac cagcgggccc cggaagcgtt cctccacgtc
      481 cgtcgtcatc cttcgaccct accgctgcgg cggagcgacg tcgcggccgt cgtatcctcc
      541 gcggatgcgt gagcgcctcc aggccctggc cgaccgcgac gtcgagcgct ggcctcagat
      601 gccggggcgc ttgatccacg tgctggcccc cgagtacggc ctcgacctcg aggtcgctgc
      661 cggcgtggcc gatcgagcga gcggcgaacc cctcacgccg ggatcacgct tccggatcgc
      721 gagcgtcacg aagcctttcg tcggagcctc ggcacttcgg ttggtcgaga ccgggcgcct
      781 cgacctcgac gcatcgctcg acgtcctcct ggccgaggag acgaccgagg tactgcgcga
      841 gggtgggtac gacacggcac ggatcaccct ccgccacctg atgacccaca cgagcggcat
      901 ctacgacttc gcggcctcgg cgtatgaccc gtcgatcgac gacggattcg atgccgcgat
      961 cgcggccgac ccgacccacc gctggacccg ggaggaacag ttgcgcttcg cgatcgacca
     1021 cggcgagccg tacggtccgc cgggcgccgt ctactgctac agcgacacga acgccaatct
     1081 ggtgggggaa ctgctcgagc gtgagaccgg caccacgatg ggcgccgcga tccgcgacct
     1141 ggtcggctac gaccgtctcg gaatgcacca cacgtatcag gagtcgatcg aacccgagcc
     1201 cgccgacctg cctccgtggt cccatcagta cgagcgtgac atcgacgtgc tcacgatcga
     1261 tccatcggtc gacctctacg gcggcggcgg cctggtctcc acttgcgccg atctcgcgaa
     1321 gttcttccgg gcgcttctgc tgcgaggcga ggtcttcgac agtccggcga ccctggtcac
     1381 gatggcgacg cgcctgcaag gcgtgccggc atccgaacgg ctcgcgacgg gcgacgaccc
     1441 tgatgacgct ggcatgttca tcttcagagc ttcagtgggt gggcttcgat ggtggggaca
     1501 cgacggctgg tggggcacca ccgtctacgc ctgccccgag ctcgaactca cggtcgtcgc
     1561 cggtcatcag cagccctaca tgccgaagga gttcgaccgg atggccgtgt tcgccgatgt
     1621 gatggagctg gtcctggctc attgaacggc ggttcgaccc tcgcggtaca ctccgcccca
     1681 tgagcacgta cccgatgtcg gaggaagatc gcgcgatcca ggaacgcgcc cgggggttca
     1741 tcgacgagga cctgatcccg tgggagcagt acgccgagga acacgacggc gagatccccg
     1801 aggccgagcg cggcaagcat cacgacaagg cgatcgagct cggcctgttc gcgatgaaca
     1861 tgccggtcga actcggcggc accgggatga ccaccttcca gcaggtcttg gtgtccgagc
     1921 agctcggccg cgtcacgaac gccctgggat ggtgcgtgca caccccgccg gcgtgggcgc
     1981 ccgccgtggt gagcgagcac cagatggaca cctggatcaa gccgacgatc cgcggcgaga
     2041 tgcacgagtg ctacgccatc accgaggaag gtgccggttc cgacgtcgat gcgatcgagg
     2101 ccacggcccg ccgcgacggc gacgactacg tgctcaacgg cgtgaagatg cacgtgacga
     2161 gcttcaacca cgccgcgtac tgcttcttcc aggccaagat cgtgggcggc gagcacgagg
     2221 gtgagcacgc gatgttcttc gtcgacgtcg acgcgcctgg cgtgcgggtc gtgcgcacac
     2281 ccgcctacac ccacacgtac gccgaccacc atccgctcgt cgcgttcgag gacgtccgcg
     2341 tgcccgccgc caacctggtc ggcgccgagg gcgacggcat ggcgttcacc tatgagtggt
     2401 tccggtacga gcgcctgatg atcgcggcgc ggtgctgcgg cgccgccgac cgcctcatca
     2461 ccgaggccac cgccttcgcg aaggagcgcg tgcagttcgg cgagccgatc ttcgcgttcc
     2521 aggcgatcca gcacatgctg gccgactcgc tcaccgagct gtgggcggcg cgcctcatga
     2581 cctacgaact cgcgaagaac atcgaccgcg gcgtcgacgt gaagctgcaa cacgcgcagt
     2641 gctccatggc caagctctac gcttcggaga tggctggccg tgtcgccgac cgcgcggtgc
     2701 agatcttcgg tggccggggc tacatgcgcg agaacgtcgc cgagcggttc tggcgcgagg
     2761 tccgcgtcga tcgcatctgg gagggaacct ccgaggtgca gcgcagcatc atcgccgacc
     2821 agctcggcaa gcgcggcgtc agcccgctca tcggctgatc acgcgcctct gctcagcccg
     2881 ttcgacgtcg cccggtagcg agttcagtac catcgacctc gtctgcggcg gttccgggag
     2941 gctgacccgg gctgctgggg ggaccacgca catccgagcc cgcgggaccg aacccccgcc
     3001 cgcgctggga gttgataccg atggaacgtc tcagccactg gatcggcggc aagctcgtat
     3061 ccggggagag cggccgcacc ggagccgtct tcaaccctgc caccggcgaa cagacgcacg
     3121 aggtcgactt cgccgacgtc gcggaggtcg acgccgcgat cgaggtcgct cgggaggcgt
     3181 tcgtctcctg gcgggccacc tcgttgtcca agcgtgccga catcctgttc cgcatccgcg
     3241 agtcgatcca cgagcatcgc gccgacatcg gacgcatcat cacttcccag cacggcaagg
     3301 tgctgtccga tgccatgggc gaggtcgccc gcgggctgga gaacgtggag ttcgccacgg
     3361 gcatcccgca cctgctgaag ggcgggttca ccgagcaggc gtccacgggc gtcgacgtgt
     3421 actcgatccg gcaaccgctc ggcgtcgtgg cgggcatcac cccgttcaac ttccccgcga
     3481 tggtgcccat gtggatgttc gccaacgcca tcgcgtgtgg gaacacgttc gtcctgaagc
     3541 ccagcgagaa ggacccgggc gcatccctct acgtcgccga gctgctgaag gacgccggcc
     3601 ttcccgacgg cgtgttgaac gtcgtgcagg gtgacaaggt cgtggtggac cggctcctgg
     3661 agcaccccga cgtcgaagcg gtgagcttcg tcgggtccac gccgatcgcc aagtacatct
     3721 acgccacggg caccgcgaac ggcaagcgcg tgcaagcgtt gggtggagcc aagaaccaca
     3781 tggtggtgct gcccgatgcc gacatcgaca tggcggccga cgccgccgtg agcgctgctt
     3841 acgggtcggc cggcgagcgc tgcatggcga tcagcgtcgc ggtcgcggtg ggcgatgccg
     3901 gtgaccgcct ggtggaggcc atcaaggaac ggctgcccct ggtgaagatc ggcaacgggc
     3961 tcgagcccga atcggagatg ggaccgctcg tgacgcgtga gcaccgggac aaggtggccg
     4021 gctacctcga ctcggcgaag gcgcagggcg ccacgatcgt cgaggacgga cgggagcacg
     4081 ggctgtatcg cgagagcgac ggcttcttcc tgggagtctc gctgatcgac cacgtcacca
     4141 ccgacatgga cgcctacaag gacgagatct tcgggcccgt gctcgaggtg atgcgcgtgg
     4201 acagctacga cgaagccctc gggttggtga acgacaatcc ctacgggaac ggcaccgcga
     4261 tcttcacgcg cgacggcggc gcggcgcgcg cgttccagtt cgacgcccag gcgggcatgg
     4321 tgggcgtcaa cgtgccgatc cccgtgccgg tgggctacta ctcgttcggc ggctggaagg
     4381 actcgttgtt cggcgacagc cacatgtacg gtcccgaagg cgtgaacttc tacaccaagg
     4441 gcaaggtggt caccgcgcgc tggcccgacc cgggcacgtc gagcgtcgac ctcggattcc
     4501 cccgaacacg atgaggagag ctcgatgacg gacagcgccc agggattcct ggaacgggaa
     4561 gcggcgtcgt gggatgcctt cgaggctcag gtcgatcgcg tgacggccga gcagcgcgag
     4621 gttcccggcg tcgtggaggg atggtcgctc aaggacgtgg tgtggcactg cgcctattgg
     4681 gctcggttct ccgccgatca cctggtgatg gagggcgacg gtccccatac cgaccccttc
     4741 gccaccctca cagccgagga ggcggaccgg gagaacgccg ccatcgcgga agcctcgagc
     4801 gcgatgtcct gggacgatgt gtgggagggc gcggccgacg cccgagccag cgtgcgctcc
     4861 gtgatcgcca cgccggggtt gtcgtcggag cagatcgatt tcgccgccga ggagtcctgg
     4921 gtccactacg acgaacatgc cgagcacgtg cgggcgttcg ccgacgcgct ggggtgatcg
     4981 tcaaccgccg gtgcgcatga accagatgcg cggagcgtcg tcggccgcgc gcccgtcgaa
     5041 cacggtgcgg aacccgaacc gctcgtagta ggcgacgttc gcctcgaccg cggtttcgag
     5101 gaaggcgtcg gcgccgtcgc gtgccgcgcg ttcgagtccg aaccggacca gcgcgctccc
     5161 gacgcccatg ccctgtttcg tgggatcgac gccgagcacg tcgaggtacc acgcgtcggc
     5221 aggcgtctgc gcttcgatcc acgaccagag cacgtcgtac cgagccgcgt ggtcttcggt
     5281 gagcggcgcc accagatccc gtgcggccgt gtcgctctcg aacatctgat cggacatccc
     5341 gggcgggatc cagacggcga acccgtcccc gtcgcccgcc tcccagatga ggcccaggtc
     5401 gacgatcccg aggtagatcg acccgaaggc gcgttcgatg cgttcgccga tgtcgtcacc
     5461 ggcgatcagc ggccaccgga
//
LOCUS       DATDBZ010000411         6727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_957692, whole genome shotgun sequence.
ACCESSION   DATDBZ010000411 DATDBZ010000000
VERSION     DATDBZ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957692"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..905
                     /locus_tag="VIX62_09795"
     CDS             12..905
                     /locus_tag="VIX62_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5860564.1"
                     /translation="MPERPSPLGSVDQLDAALREHSYLADRGLATAIYLSLSLGKPLL
                     LEGEAGVGKTEVAKTLSTLLDRELIRLQCYEGIDASQALYEWNYSRQLIAVRAMDAGD
                     AATHVEDLFAEEFLVQRPLLAAIRAGDRAVLLVDELDRADDEFEAFLLEILSEFAVTI
                     PEVGRVGAETPPAVIITSNRTRELHDALKRRCLYHWIDHPALEREVDIVRTRAPHVTE
                     ALARDVAEAVARLREMDLAKLPGVAETIDWANALAFLGADRLTEPLAADTLGSVVKDH
                     EDQELVTSRLSQVVGADAVDG"
     gene            898..2100
                     /locus_tag="VIX62_09800"
     CDS             898..2100
                     /locus_tag="VIX62_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018890558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5860565.1"
                     /translation="MADSVSSGPVPQLVAFGRGLRARGLPVGTGRILTFVRAVAALGL
                     TDRTSLYWAGRTSLVARRDDLATYDLAFDDWYRSLGTNGELAIELSLPAVGPEPSFDW
                     GDEPDGLEVTVGSTAAEWHNLGPEDDEEETDGESSIRIVASGNEVLRTKSFGQLTDDE
                     RARVWAMVRALTVSVPRERTRRTRPSAKGGRFDVRRTLRRSLRTQGEPFDRAWRDRTT
                     RNRPLVLILDISGSMAPYSRALMQFAYAAMAAGRRVETFVFGTRLTRVTRTLRTKDPD
                     RALAEIGAQVEDWEGGTRIGESLKTLLDGWSQRAALRGAVVVLCSDGLERGDPDLLAV
                     QMARLRRLAYRVVWVNPLKGSPRYEPLARGMAAALPSVDVFLSGHNLESLEHLGRVLG
                     AEKRLGRR"
     gene            2110..2616
                     /locus_tag="VIX62_09805"
     CDS             2110..2616
                     /locus_tag="VIX62_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860566.1"
                     /translation="MHDDDEILETYEHEFEFDAEPAPRRSNRGFWLVAGSILVGSIVL
                     VVEIFANRPIANTIGHAQFDLRAAQSAALKISSTSGSFAGANADGLNLERLDEGRLIA
                     VGPDEVSDGVNEVSVYADEDTWAAAVSARPGACFYLKITTTEESPLYGVGTTCTGRAA
                     LTATDTRW"
     gene            complement(2617..3189)
                     /locus_tag="VIX62_09810"
     CDS             complement(2617..3189)
                     /locus_tag="VIX62_09810"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY5860567.1"
                     /translation="MRYISVGRRFAALLLDWIILLVVAIPLAEIHSGNGSVEISWLGW
                     RFALAWIAVPLTYVVLFEWLASATPGKFMVGIRVRKDTGGRIGFGQSLGRNVARLIDG
                     LPYVIPYLVGGLVVLGSDTRQRLGDRWASTVVIAKGTDAAGAIAPPAPMTEEPWTGPL
                     PPAPPGSLTGSQPTDPHVDGTPTPPPPPEV"
     gene            3369..4673
                     /locus_tag="VIX62_09815"
     CDS             3369..4673
                     /locus_tag="VIX62_09815"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEY5860568.1"
                     /translation="MALTTGVTLATVASSEVTSATARPHTARTSSTQTLAPGLTLTKI
                     TDPAGPWILRVLTIDPSKPLTIDIATAGGEIGSYARPSVIGDTRGALAAINGDFTVSP
                     GRPLHPFAEDGALKELGLQNGASFAIAQDESAAYIDTERVAGSGKNVATKRSFSIAGW
                     NTGAPKGASIAAFTAYGGQAERPPSDACSVRLKPAGKMLFGKSGVGVVRDYTVVRRRC
                     LAAPMGMRSGTVVLSSKMSGTGAVAIKEMKKRQHIRLKWSFGWKGVMDSVGGMPILVK
                     DGKNVANTCSSYFCSRNPRTGIGVTADGHILLVTVDGRKSSSVGMTLIGFAGYLRDLG
                     AVNAVNLDGGGGSTMWVKGQGIVNDPSDSGGERAVTNVVLVLPGADPGEPVMSRVSAQ
                     LGLTVGGEAMPTLLSRSAARRTQAASATDPGSTGGLLQAFGL"
     gene            complement(4670..4948)
                     /locus_tag="VIX62_09820"
     CDS             complement(4670..4948)
                     /locus_tag="VIX62_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5860569.1"
                     /translation="MLRQVPGMEVVEIANGDRCCGAAGLYNVTEPEMSRRLMREKAEA
                     VRDTGAAIVASANPGCTLQLRAGLRELGADVEVVHPVQLLDRAGDGTV"
     gene            complement(4964..5944)
                     /locus_tag="VIX62_09825"
     CDS             complement(4964..5944)
                     /locus_tag="VIX62_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5860570.1"
                     /translation="MPIDDLPVTPLGTEMPEGTRFIGREPGSTANWRATDHPADVDLD
                     SCVACGLCLPHCPTYRLTGEESASPRGRIAAMRSVADGVAEVDDTFASFMDLCLVCRA
                     CEDVCPSHVPFGRMMEAARVQIEPMRSHRARFARWLGLDVALPHKKLLWMAAVLQPVV
                     RPFMPRRIRDITPKPSARFRRLPARTEPPDGVALRGTVAMLSGCVQDRWFRDVNEATI
                     RVLARNGWRVLVPRDQVCCGALQAHTGRLDTARRLARRNAAAFAGADQVVVNAAGCGA
                     HMRDYGDLALPVRDLMAFLYEKGLTSAPNDGDQVTVAYHDACHALRAQHV"
     gene            6022..>6727
                     /locus_tag="VIX62_09830"
     CDS             6022..>6727
                     /locus_tag="VIX62_09830"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEY5860571.1"
                     /translation="MTEPEGRERRFGRLTEQAEEGLLTYGGYLKVPELLALQQVQSDP
                     TVHDELLFIVVHQAYELWFRQLLFELESIRDEMFEGHPEQARHLLTRVHAIERVVIEH
                     IAVLQTMTPQDFLEFRAVLTPASGFQSAQFREVEFVSGLKDERFLQDMATSEPEHDRL
                     VKRLDEPTLWDGFCALMEARGLPMPADDEDARMASLVAMAREHPELFAVSEGLVDHDE
                     SIAQWRYLHVLMVEREI"
BASE COUNT         1040 a         2330 c         2357 g         1000 t
ORIGIN      
        1 agcatgagcc gatgcccgag cgtccttcgc ccctgggttc ggtcgaccaa ctcgatgccg
       61 ccttgcgcga gcactcctac ctcgccgacc gcggcctcgc caccgcgatc tacctgtcgc
      121 tgtcgctcgg caagccgttg ctgctcgaag gcgaggcggg ggtgggcaag accgaggtgg
      181 ccaagacgct ctccaccctg ctcgaccgcg agctgatccg tctgcagtgc tacgagggca
      241 tcgacgcctc gcaggccctc tacgaatgga actactcgcg ccagctgatc gcggtccgcg
      301 ccatggatgc cggcgacgcc gcgacccacg tcgaggacct gttcgccgag gagttcctgg
      361 tgcagcgacc gttgctggcg gcgatccgtg cgggcgatcg cgcggtgttg ctggtggacg
      421 aactcgaccg cgccgacgac gagttcgagg cgttcctgct cgagatcctc agcgagttcg
      481 cggtcacgat ccctgaggtg ggccgtgtcg gcgccgagac gccgccggcg gtgatcatca
      541 cgtcgaaccg cacgcgcgag ctgcacgacg cgttgaagcg ccgctgcctg taccactgga
      601 tcgatcaccc cgcgctcgag cgagaggtcg acatcgtgcg gacgcgcgcg ccgcacgtca
      661 ccgaggcgct ggcccgcgac gtcgccgagg cggtcgcccg gctccgtgag atggacctgg
      721 cgaagctgcc tggcgtggcc gaaacgatcg actgggccaa cgcgctcgcc ttcctggggg
      781 ccgaccgcct caccgaaccg ctcgccgccg acacgctcgg ctccgtcgtg aaggaccacg
      841 aggatcagga gctcgtgacg agccggctct cacaggtcgt cggcgccgac gccgtcgatg
      901 gctgactccg tctcgtccgg tccggttccc cagctcgtcg cgttcggccg cgggctccgc
      961 gcgcggggat tgcccgtggg cacggggcgc atcctcacgt tcgtccgcgc ggtggcggcg
     1021 ttgggactga ccgatcgcac gtcgctctat tgggccggcc ggacgtcgct ggtggcgcgc
     1081 cgtgacgacc tcgcgaccta cgacctggcg ttcgacgact ggtaccgctc cctgggcacc
     1141 aacggggaac tggcgatcga actctcgctc cccgccgtcg gccccgagcc gtcgttcgac
     1201 tggggggacg agcccgatgg tctcgaggtc accgtcgggt ccacggccgc cgaatggcac
     1261 aacctcggcc ccgaggacga cgaggaggag accgacggcg agagctcgat ccggatcgtg
     1321 gcgagcggca acgaggtgct gcggacgaaa tcgttcggcc agctcaccga cgacgagcgc
     1381 gcccgggtgt gggccatggt tcgcgcgctc acggtcagcg tgccccgaga acgcacccgc
     1441 cggacccggc cctcggccaa gggggggcgg ttcgacgtcc ggcggacgct gcggcgctcg
     1501 ctgcgcaccc agggggaacc gttcgaccgg gcctggcgcg accgcaccac acgcaaccgc
     1561 ccgctcgtgc tcatcctgga catcagcggg tccatggcgc cgtactcgcg ggcgctgatg
     1621 cagttcgcgt acgccgcgat ggctgccggc cggcgcgtcg agacgttcgt gttcggcaca
     1681 cggctcacac gcgtcacccg gacgctgcga acgaaggatc ccgatcgcgc tctcgccgag
     1741 atcggcgcgc aggtcgagga ctgggaaggc ggcacgcgca tcggcgagag cctcaagacg
     1801 ttgctggacg gatggagtca gcgggcggcg ctccgcggtg ccgtcgtggt gctgtgttcc
     1861 gacgggctcg agcggggcga ccccgacctc ctcgccgtgc agatggcccg attgcgccgg
     1921 ctcgcgtacc gggtggtgtg ggtcaacccg ctgaagggat cgccgcgcta cgaaccgctc
     1981 gcccgcggca tggcggcggc gctgcccagc gtcgacgtgt tcctttctgg gcacaacctc
     2041 gagagcctcg agcacctggg ccgggtcctc ggcgcagaga agcgcctcgg acggcggtag
     2101 gctgttcgcg tgcatgacga cgacgagatc ctcgagacct acgagcacga gttcgagttc
     2161 gacgccgaac ccgcccctcg caggtcgaac cgcggattct ggctggtggc cgggtcgatc
     2221 ctggtcgggt ccatcgtgtt ggtcgtggag atcttcgcga accggcccat cgccaatacg
     2281 atcggccacg cgcagttcga cctccgtgcg gcgcagtcgg cggctttgaa gatcagcagc
     2341 acgtcgggtt cgttcgccgg agccaacgcc gacggcctga acctggagcg gctggacgag
     2401 ggccggctca tcgcggtggg acctgacgag gtctcggacg gggtcaacga ggtcagcgtg
     2461 tacgccgacg aggacacctg ggccgcggcg gtgagcgccc gccccggcgc ctgcttctac
     2521 ctgaagatca ccaccaccga ggagagcccg ctgtacggcg tgggcaccac ctgcaccggg
     2581 cgagcggcgc tcacggccac cgacacccgc tggtagtcag acctccggcg ggggaggcgg
     2641 cgtcggcgtc ccatccacgt gcgggtcggt cggctgcgaa cctgtcagcg aaccgggcgg
     2701 tgccggcggc agtggtccgg tccacggctc ttcggtcatc ggtgccggcg gtgcgatcgc
     2761 tcccgcagcg tctgttccct tcgcgatgac caccgtcgag gcccagcggt cgccgagccg
     2821 ttgccgggtg tcggacccga gcacgacgag gccgccgacc aggtacggga tcacgtaggg
     2881 gaggccgtcg atcaggcggg cgacgttccg gcccagcgac tgaccgaacc cgatcctgcc
     2941 gccggtgtcc ttgcgcaccc tgatgcccac catgaacttg cccggcgtgg cgctggcgag
     3001 ccactcgaac agcaccacgt aggtcagcgg gaccgcgatc cacgccagcg cgaaccgcca
     3061 gcccagccac gagatctcga cgctgccgtt gccggagtgg atctcggcca acgggatcgc
     3121 caccacgagc aggatgatcc aatcgagcag cagggcggcg aaccgacggc cgacgctgat
     3181 gtacctcatg ggggtgtcct ccaaccgggg aagccgcttc ccgacgcttc ggaccgtagg
     3241 agccgtggcc aggaaggtca agccacgcgc aaggatcgct gcgtcgcagg acttcctcct
     3301 gcatcaaggt cgatggcccg aacgccgatg actagtccat gcgagggata cgggcgaccg
     3361 cctgtgcgat ggccctgacg acgggggtga cgctggccac ggtggcctcg tccgaggtca
     3421 cctcggccac cgccaggccg cataccgcga ggacctcatc cacccagacc ctggcgcccg
     3481 ggttgacgct gacgaagatc accgatcccg ccggtccgtg gatcctgcgg gtgctgacga
     3541 tcgatccgtc caaaccactc acgatcgaca tcgcgacggc cggcggcgag atcggttcct
     3601 acgcgcgacc gagcgtgatc ggcgacacac gcggagcgct cgcggcgatc aacggtgact
     3661 tcacggtctc cccggggcgc ccactgcacc cgttcgccga ggacggcgcc ctgaaggagc
     3721 tcggtctgca gaacggggcc agcttcgcga tcgcccagga cgaatccgcc gcctacatcg
     3781 acaccgaacg cgtggctggc agcggcaaga acgtcgccac caagcggtcg ttctcgatcg
     3841 ccggatggaa caccggcgct ccgaagggcg cgagcatcgc ggccttcacg gcgtacgggg
     3901 ggcaggccga gcgtcccccg tcggacgcct gctcggtccg cctcaagccc gcaggcaaga
     3961 tgctgttcgg caagagcggc gtcggtgtgg tgcgcgacta caccgtggtg cgccgtcgat
     4021 gcctcgcggc gcccatgggc atgcggagcg gcaccgtcgt gctctcctcg aagatgagcg
     4081 gcaccggcgc cgtcgcgatc aaggagatga agaagcgtca acacatccgc ctgaagtggt
     4141 cgttcggatg gaaaggcgtg atggactcgg tcggcgggat gccgatcctg gtcaaggacg
     4201 gcaagaacgt cgcgaacacc tgcagctcct acttctgctc ccgcaacccg cggaccggca
     4261 tcggcgtcac cgccgacggg cacatcctgc tggtcaccgt cgacggccgg aagagctcgt
     4321 cggtggggat gaccctgatc gggttcgcgg gctacctccg cgatctcggc gccgtgaacg
     4381 ccgtgaacct ggacgggggc ggcggctcga cgatgtgggt gaaaggccaa ggcatcgtga
     4441 acgacccctc ggactccggc ggcgaacgag ccgtgacgaa cgtggtcctg gtgctgccgg
     4501 gagcagatcc cggcgaaccc gtcatgtcgc gggtgagcgc acagctgggc ctcaccgtgg
     4561 gtggtgaggc gatgccgacg ctgctctccc gctccgccgc tcgtcgcacg caggcagcct
     4621 cggccaccga ccccgggtcc accggcggcc tgctgcaggc gttcggtctc tagacggttc
     4681 cgtcccccgc tcggtcgagc aattgcaccg ggtgcaccac ctcgacgtcg gcgcccagtt
     4741 cacgcaggcc cgcacggagt tgcagcgtgc accctggatt cgcgctcgcg acgatcgcag
     4801 cgccggtgtc ccgaacggct tcggccttct cgcgcatgag gcgccgtgac atctcgggct
     4861 cggtgacgtt gtagagcccg gcggcgccac agcagcggtc tccgttcgcg atctcgacga
     4921 cctccatccc ggggacctgc cgtagcagcg ccctcggctc cgcctacacg tgctgggcgc
     4981 gcagggcatg gcaggcgtcg tggtaggcga ccgtcacctg gtcaccgtcg ttcggtgccg
     5041 aggtcagtcc cttctcgtac aggaacgcca tcaagtcgcg aaccgggagc gcgaggtcgc
     5101 cgtagtcgcg catgtgggcg ccgcaccccg ccgcgttgac cacgacctga tccgcgccgg
     5161 cgaaggccgc cgcgttgcgt cgcgccaggc gacgggcggt gtccagccgt ccggtatgag
     5221 cctggagcgc gccgcagcac acttggtcgc ggggtaccag cacacgccac ccgttgcgcg
     5281 cgagcacgcg gatcgtggcc tcgttgacgt cgcggaacca acggtcctgg acacagcccg
     5341 acagcatcgc gaccgtgccc ctcagggcga cgccgtcggg cggctcggtc ctggcgggta
     5401 gccggcggaa cctcgcgctg ggcttcggcg tgatgtcgcg gatgcgcctg ggcatgaacg
     5461 ggcgcaccac gggttggagg acggccgcca tccacagcag cttcttgtgg gggagcgcga
     5521 cgtcgagccc cagccagcgg gcgaagcgcg cccggtgcga tcgcatcggc tcgatctgca
     5581 cccgcgccgc ttccatcatc cggccgaacg gcacgtgcga cggacacacg tcctcgcagg
     5641 cacgacacac caggcacaga tccatgaacg aggcgaacgt gtcgtcgacc tcggcgacgc
     5701 cgtcggccac cgagcgcatc gcggcgatcc gtccccgggg cgaggccgac tcctcgccgg
     5761 tcaaccggta ggtggggcag tgcggcagac acagcccgca cgccacgcac gagtcgaggt
     5821 cgacgtcggc gggatggtcg gtggcgcgcc agttcgccgt cgagcccggt tcgcggccga
     5881 tgaaccgggt gccttcgggc atctcggtcc ccagcggggt cacggggagg tcgtcgatgg
     5941 gcacgttccc atcctcacac gccgacggcg cagtggctgc ccgtttgacc ctggctcgtc
     6001 cggcacctta cggtcccgct catgaccgag ccggagggac gtgagcgacg cttcggccgg
     6061 ctcaccgagc aggccgagga agggctcctc acctacggcg gctacctgaa ggtgcccgaa
     6121 ctgctggcat tgcagcaggt gcagtccgat cccaccgtgc acgacgagct gttgttcatc
     6181 gtggtgcacc aggcgtacga gctctggttc cgtcagctgt tgttcgagct ggagtcgatc
     6241 cgcgacgaga tgttcgaggg gcatccggag caggcgcggc acctgttgac ccgggtgcat
     6301 gccatcgagc gggtggtgat cgagcacatc gcggtgctgc agacgatgac gccgcaggac
     6361 ttcctggagt tccgcgccgt gctcacgccg gccagcggat tccagtcggc gcagttccgg
     6421 gaggtggagt tcgtctcggg gttgaaggac gaacgcttcc tgcaggacat ggcgacgtcg
     6481 gagccagaac acgaccgatt ggtgaagcgc cttgacgaac ccacgctctg ggacggtttc
     6541 tgtgcgctga tggaagcccg tgggctgccg atgccggccg acgacgagga cgcccgcatg
     6601 gcctcgctgg tggcaatggc acgcgaacat ccggagttgt tcgcggtgag cgaaggcctc
     6661 gtggatcacg acgaatcgat cgcgcagtgg cgctacctcc acgtgctgat ggtcgaacgc
     6721 gagatcg
//
LOCUS       DATDBZ010000412         2247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1777964, whole genome shotgun sequence.
ACCESSION   DATDBZ010000412 DATDBZ010000000
VERSION     DATDBZ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777964"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1079
                     /locus_tag="VIX62_09835"
     CDS             78..1079
                     /locus_tag="VIX62_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5860572.1"
                     /translation="MTSDIDIDHAEPAKAPGRQAATAAFRKLQNNIEKVLRGKTDTVR
                     LALCCMLAEGHLLIEDPPGTGKTSLAKAIAASIGGDVRRVQFTPDLLPSDITGVSIYN
                     PVHGDFSFRKGPVFTNVLVADEINRASPKTQAALLEVMEERQVTADGESRMVPRPFMV
                     VATQNPLDFQGTYPLPEVQLDRFAMKISLGYANRDAELEVMARQDGRDLIAELDPVLD
                     QHSMAALIQRVRTVNVAVPLREYVADIVERSRRTRELRLAISTRGTLTLLSVARAYAL
                     AEGRNFITPDDIKALYQAVLQHRIAVTPEAELDGITEADVLDEIVDSLPVPRSRQTT"
     gene            1076..2224
                     /locus_tag="VIX62_09840"
     CDS             1076..2224
                     /locus_tag="VIX62_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5860573.1"
                     /translation="MSSRWRLTSSGTALGVGVGSLLALGVIARYDELIVFAAAGALLL
                     GLAFLVPRVTSPITIERTEVPRMVERGTIVQIGLRASADGVVPPVRIIDQLAGVSVPI
                     DLPQIDVAKPVHVRYRIQAVRRGVHTVGPMLEERSDPFTLAVRTTEHDVRDDIIVHPV
                     VHNLKFPAGGARQRERNTMVSRISQDPLADFRALRDYVPGDDPRNVHWASTARTGRLV
                     VKDHLEMRKSMRLVVLETLDRTITGPLFEEAVEIAVSIVIDAVTEGISVTARTRDAAH
                     AGRLETITGRREALELFARVQRTESLSTLGADRVRPRGQDNDQVFLIAGGSSPLIDGF
                     ATNVWVRHRLVVIRVSERPAELRKLPVRKIDVRNAEEFVGRWRMGMVT"
BASE COUNT          388 a          782 c          722 g          355 t
ORIGIN      
        1 ggaacgattg tgctctcata caccatcaac gatggccacg gcggctccgc cagcggaaag
       61 atcacgatca acgtctcatg accagcgaca tcgacatcga ccacgccgag ccggcaaagg
      121 ctccggggcg ccaggctgct acagccgcgt tccggaagct gcagaacaac atcgaaaagg
      181 tgctcagggg caagaccgac acggtccgcc tcgccctgtg ctgcatgctg gccgagggcc
      241 atctcctcat cgaggatccg cccggcaccg gcaagacctc gctggcgaag gcgatcgcgg
      301 cgtccatcgg cggtgatgtc cgccgcgtgc agttcacccc cgaccttctg ccgtccgaca
      361 tcacgggggt ctcgatctac aaccccgtcc atggcgactt cagcttccgc aagggtccgg
      421 tgttcaccaa cgtgctcgtg gccgacgaga tcaaccgcgc ctcgccgaag acccaggccg
      481 ccctgctcga ggtgatggag gaacgccaag tcacggcgga cggcgagagc cgcatggtgc
      541 cccggccgtt catggtcgtg gccacccaga acccgctcga cttccagggt acgtatccgt
      601 tgcccgaggt ccagctcgac cgcttcgcga tgaagatcag cctgggctac gccaaccggg
      661 atgccgaact cgaggtcatg gcccgtcagg acggccgtga cctcatcgct gaactggacc
      721 cggtgctcga ccagcacagc atggcggcgt tgatccagcg tgtccgtacc gtcaacgtgg
      781 cggtgccgtt gcgggagtac gtggccgaca tcgtcgagcg cagccgacga acgcgcgagc
      841 tccgcctcgc catcagcacc cgtggcacgc tgaccctgct gtcggtcgca cgggcctacg
      901 cgctggcgga agggcgcaac ttcatcaccc ccgacgacat caaggcgctg taccaggcgg
      961 tgctccagca ccgcatcgca gtcacgccag aagccgagct cgacggcatc acggaagccg
     1021 acgtgctcga cgagatcgtc gacagtctgc cggtgccccg ctcccgacag accacatgag
     1081 ctcacgctgg cgcctgacct ccagcggcac tgcgttggga gtcggtgtgg ggagcttgct
     1141 cgcgctcggg gtgatcgccc gctacgacga gctcatcgtg tttgcggcag cgggcgcctt
     1201 gttgctcggg ctcgccttcc tcgtgccccg cgtcacgtcg ccgatcacca tcgagcgcac
     1261 ggaggtgccg cgcatggtcg agcgcgggac gatcgtccag atcggcctgc gggccagcgc
     1321 cgatggtgtc gtgccgcccg tgcgcatcat cgaccagctc gcgggcgtct cggtaccgat
     1381 cgacctgccg cagatcgacg tggcgaaacc ggtccacgtc cgctaccgca tccaggccgt
     1441 acgccgaggg gtccacacgg tcggccccat gctcgaggaa cgcagcgatc ccttcacgct
     1501 tgccgttcgc acgaccgagc acgacgtacg cgacgacatc atcgtgcacc ccgtcgtgca
     1561 caatctgaag ttcccggccg gaggggcgcg gcagcgggag cgcaacacga tggtgtcgag
     1621 gatcagccag gacccattgg ccgattttcg ggcgctgcgc gattacgtcc ccggcgacga
     1681 ccctcgcaac gtccactggg cgagcacggc gcggaccggt cggctcgtgg tcaaggacca
     1741 cctcgagatg cgcaagtcga tgcggctggt cgtactcgag acgctcgacc gtacgatcac
     1801 cgggcccttg ttcgaggaag ccgtcgaaat tgcggtcagc atcgtcatcg acgccgtcac
     1861 cgaggggatt tcggtcaccg cccggacgag ggacgccgca cacgccggac ggctcgaaac
     1921 gatcaccggc cggcgcgagg cgctcgagct cttcgctcgg gttcaacgca ccgagtcgct
     1981 gtccacgttg ggtgccgatc gggtgcgccc ccgcggccag gacaacgatc aggtcttcct
     2041 gatcgccggg ggttcttcgc cgctcatcga tggctttgcc accaacgtct gggtccgcca
     2101 tcggctcgtg gtcatcagag tcagcgagcg accggctgag ctgcgcaagc tccccgtgcg
     2161 caagatcgat gtccgcaatg ccgaggagtt cgtcggtcgc tggcgcatgg ggatggtcac
     2221 gtgaggatcg gccaccacct cagggtc
//
LOCUS       DATDBZ010000413         6342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2365432, whole genome shotgun sequence.
ACCESSION   DATDBZ010000413 DATDBZ010000000
VERSION     DATDBZ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365432"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VIX62_09845"
     CDS             <1..314
                     /locus_tag="VIX62_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860574.1"
                     /translation="MSARYRWVLAQDEPPLVGYDQDLWVDHLHTEAAQESPESLLAVF
                     EPLRRANVDLWRRTPVESRSRVGQHAERGPESYDLAFRMIAGHDRFHLAQAERALAAV
                     R"
     gene            complement(500..988)
                     /locus_tag="VIX62_09850"
     CDS             complement(500..988)
                     /locus_tag="VIX62_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5860575.1"
                     /translation="MSARSKRTVAWEVWRLMARFTMEKFQRGEHLTLLRERGLTPGHM
                     KALTILDPDLPRPMGVMADMMNCDASQVTWLVDRLEERGFVERRALPTDRRVKTIALT
                     PSGVVFRAELLEHMFEPPPELMEVDAATLESLREALEQLPAPTDGYWFGLDRRPGAAP
                     RA"
     gene            1090..2085
                     /locus_tag="VIX62_09855"
     CDS             1090..2085
                     /locus_tag="VIX62_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004921168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5860576.1"
                     /translation="MTDNAIVVEGIKKHYDEVVALDGVDLEVEAGTVFGLLGPNGAGK
                     TTMVRVLATVLDPDEGRAEVLGYDVMKQADAVRRRIGLAGQYAAVDANLTGRENLQMV
                     GVLSQMQNKEIAPRAAELLGAFDLTDAADRPVRTYSGGMRRRLDIAASLVHRPPILFL
                     DEPTTGLDITSRNALWDMIRDLVDHGTTVLLTTQYLEEADQLASRIAVVDGGKVIAND
                     TPVALKADLGSTVVEMEMSDDQVAARAVQLVAGTFEGRAESEGALVRIASDQGAHALV
                     EVLRALDAQHLEPITLVVREPSMDDVFLTLTGHRAESPTDEEPSPPASRSSRGAA"
     gene            2082..2894
                     /locus_tag="VIX62_09860"
     CDS             2082..2894
                     /locus_tag="VIX62_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860577.1"
                     /translation="MSSTAVPATADIARDRTIPFSLVRDTLAITKRNLIAYRRVPQLL
                     VFSTIQPVIFVLMFTYVFGGAIDTGPIPYVDYLMPGIFVQVVVFGSIATAIGLASDLK
                     SGLLERFRSLPMGRSAVLTGRTFADLTRNVFVVTLMMIVGFLVGWRIHTNVFGLIAGG
                     LLVLLFGYSMSWIFATVGLAMGDPETAQAAAFPVLAPLVFASSAFVPVDSMPSWLQPF
                     AEHQPVSVTASAVRALTLGGPTASWVLQSIAWTVVILAVFGPWAVRIYRKSV"
     gene            2902..3489
                     /locus_tag="VIX62_09865"
     CDS             2902..3489
                     /locus_tag="VIX62_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY5860578.1"
                     /translation="MAFDGELVTARDRAVWRRWLQRHHASRDGAFVEFHKKSSASRSI
                     TYEEAVEEALCFGWIDSRPKKLDDDRWALWFAPRKPKSVWSAINKERVERLIADGAMT
                     EAGLAAIAVAKANGAWDQLNDSDALLVPDDLSAALDEHVDARRHWDAFPPSVRKQILG
                     WINSAKREDTRAKRVEETATSAAENVRAHQWRPKA"
     gene            3526..4830
                     /locus_tag="VIX62_09870"
     CDS             3526..4830
                     /locus_tag="VIX62_09870"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5860579.1"
                     /translation="MSRRSDSLARPLLIFAGLALVALTVVAVTGALVVRKAATDQALA
                     EARQLTDLSARIVEQRVNDGLLTGDAETLGAVASVVFDAVKTYPIVRVKIWDRDGTIL
                     YSDQTELIGSTYDLGEDELEVIDHGGVVADVSDLSEPENRFEQDFGELLEVYTRIDTH
                     RKGEGTPLLFETYQLASSIADRRRELTATFLPILVVTLAVFVLLMVPIAWFLVRRVRA
                     SQLERERLMQRALDASEREQRRIAGDLHDGPVQEMAGLAMRLSAEAELVDDADAGITL
                     RSSASAVRGSVRMLRSAIVEIYPPNLQQAGLPAALSDLVTGLDRHGIELSLEVEPDAG
                     FGAEVDALLYRATQEALRNVEEHADARHVRVVITRQGGRAVLEVADDGRGIAPDALAE
                     APPRGHMGLAILGDMVSDAGGTLTVEPGVQAGTVVRVEVQAP"
     gene            4827..5453
                     /locus_tag="VIX62_09875"
     CDS             4827..5453
                     /locus_tag="VIX62_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860580.1"
                     /translation="MITVAIADDHRVVRVGLEQLLTTFDDVSFVGAGAGGREAVAICE
                     AHHPDVLLLDLSMPDLDGIEATAQVRVVSPDTRVVVFTSFSDRDRIVRALDNGAIGYL
                     LKDAEPEEIHAAIQAASRGESPLAPRAAAELLADRRSRTGDVELTAREREVLGLVVDG
                     LANKQIARRMGISEKTVKGHLTNLFQRIGVADRTQAALWAERTGALRD"
     gene            5510..5881
                     /locus_tag="VIX62_09880"
     CDS             5510..5881
                     /locus_tag="VIX62_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860581.1"
                     /translation="MRRMLAIALVFAVLVATPAVAGGGSDRRGSCSGGPGHWRLRVQR
                     EDADTLRVRFRIEDVAPGQSWQLFISDNGTRIYSATRTSTSDGEIRVRKNTRDRAGRD
                     HIAANAVNSATGTTCEGSVSI"
     gene            5980..>6342
                     /locus_tag="VIX62_09885"
     CDS             5980..>6342
                     /locus_tag="VIX62_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860582.1"
                     /translation="MISKVLRVVLASGLALSLAGAVPAHANEDDVIRRGNCSGSSDWK
                     LKLSPEDGRIEVEFEVDSNVVGQTWQVRLTKNGNQIFSGTRVTRGASGSFELRRVTSD
                     PKGDDVFRAHATHAGEVCA"
BASE COUNT         1007 a         2165 c         2157 g         1013 t
ORIGIN      
        1 tcatgagcgc gcgctaccgc tgggtgctgg cgcaggacga accacccctg gtcggctacg
       61 accaggacct gtgggtcgat cacctgcaca ccgaagcggc tcaggaatcg ccggagtcgt
      121 tgctggcggt cttcgagcca ctgcgccggg cgaacgtcga cctgtggcga cgcaccccgg
      181 tcgagtcacg gtcgcgggtg gggcagcacg ccgagcgtgg accggagagt tacgacctgg
      241 ccttccgcat gatcgccggt cacgaccggt tccacctggc gcaagccgaa cgcgcgctcg
      301 cagccgtccg ctagggcgcg ccgttgccgg tcttcatacc ggaatcgaga tcgatccgcg
      361 cgatcgggtt gctgacctca actgcatccc tggatcggta tgcgagacga ctcctggaac
      421 gcccgcggtc catgtatcgc tgaaaaagga tgacgagcag gagcgctggt cacgtcgcgc
      481 gaacgtcagg ccggcacggt catgcccgcg gcgccgcgcc ggggcgccgg tccagcccga
      541 accaatagcc gtccgtgggc gccggcagct gctccagcgc ttcgcgcaac gactcgaggg
      601 tggcggcgtc gacctccatc agctccggcg gcggttcgaa catgtgctcc agcagctccg
      661 cgcggaacac cacgccggag ggcgtgaggg cgatcgtctt cactcggcgg tcggtgggca
      721 acgcgcgacg ctccacgaag ccacgttcct cgaggcgatc gacgagccag gtcacctgcg
      781 acgcgtcgca gttcatcatg tcggccatca cgcccatggg acggggcagg tcgggatcga
      841 ggatggtgag cgccttcatg tgaccggggg tgaggccgcg ttcacgcagc agggtcaggt
      901 gctcgccacg ttggaacttc tccatcgtga accgagccat caggcgccag acctcccagg
      961 cgacggttcg cttcgatcgc gcgctcacac ccccatccta cggggttcgt cgcgatactt
     1021 caagttcgtc aacctttgag ctctagaaat agttgacata tcccaaggac ctgtgccaga
     1081 gtcgcccgca tgactgacaa cgcgatcgtg gtggaaggga tcaagaagca ctacgacgag
     1141 gtggtcgccc tggacggggt cgacctcgag gtcgaggccg gcacggtgtt cgggctgctc
     1201 gggcccaacg gagctggcaa gacgaccatg gtgcgggtgc tggccaccgt gctcgacccc
     1261 gatgagggcc gagccgaggt cctgggatac gacgtgatga agcaggccga cgccgttcgt
     1321 cgccggatcg ggctcgccgg gcagtacgcc gccgtcgatg ccaatctcac cggtcgcgag
     1381 aacctccaga tggtgggcgt cctcagccag atgcagaaca aggagatcgc gccgcgcgcc
     1441 gcggagttgc tcggcgcctt cgacctcacc gacgccgccg atcgcccggt ccggacgtat
     1501 tcgggcggga tgcggcgtcg cctggacatc gcagcttcgc tcgtccaccg cccgccgatc
     1561 ctgttcctgg acgaacccac gaccggcttg gatatcacca gccgcaacgc gttgtgggac
     1621 atgatccgtg acctggtcga ccatggcacc acggtgctcc tcaccaccca gtatctggag
     1681 gaagccgacc aactggcgtc tcggatcgcg gtggtcgacg gcggcaaggt catcgccaac
     1741 gacacaccgg tcgccctgaa ggccgacctc ggcagcacgg tggtcgagat ggagatgagc
     1801 gatgaccagg tcgccgcacg cgccgtgcag ctcgtggccg gcacgttcga gggtcgggcg
     1861 gaatccgaag gcgcgctggt ccggatcgcg tccgatcaag gcgcacacgc gctggtcgag
     1921 gtgctccgcg cgctcgatgc ccaacacctt gaaccgatca cgctcgtcgt ccgtgagccc
     1981 agcatggacg acgtgttcct gaccctgacg ggccaccggg ccgaatcccc caccgacgaa
     2041 gaaccgtcgc cacccgcgtc ccgatcctcg agaggtgccg catgagttcc accgccgtcc
     2101 ccgccaccgc cgacatcgcg cgtgaccgca cgatcccgtt ctcgctggtc cgcgacacgc
     2161 tggcgatcac gaagcgcaac ctgatcgcct atcgtcgcgt gccacagttg ctcgtcttct
     2221 ccacgatcca gccggtgatc ttcgtcctga tgttcacgta cgtattcggc ggcgcgatcg
     2281 acacgggccc gatcccgtac gtcgactacc tgatgccggg gatcttcgta caggtcgtgg
     2341 tgttcggctc gatcgccacg gccatcggcc tcgcctccga cctgaagagc gggttgctgg
     2401 agcgtttccg ctccctgccg atgggtcgct cggcggtgtt gaccggccgg acgttcgccg
     2461 acctcacgcg caatgtcttc gtggtgacgc tgatgatgat cgtcggcttc ctcgtggggt
     2521 ggcgaatcca cacgaacgtg ttcgggctga tcgcgggtgg gctgctggtg ctcctgttcg
     2581 gctactcgat gtcgtggatc ttcgccaccg tcggcctggc gatgggcgat ccggagacgg
     2641 cccaggccgc cgccttcccg gtcctcgcgc cgctggtgtt cgcctcgtct gcgttcgtac
     2701 cggtcgattc gatgccgtcg tggttgcagc ctttcgccga gcatcagccc gtgtcggtga
     2761 cggcgtcggc cgtccgcgcg ctcaccctcg gcgggcccac cgccagctgg gtactgcaat
     2821 cgatcgcttg gacggtcgtg atcttggcgg tcttcggtcc ctgggcggtg cggatctacc
     2881 gcaagtcggt ctgacgcggg catggctttc gacggcgaac tcgttaccgc gcgcgatcgc
     2941 gccgtctggc ggcggtggct gcagcgccac cacgcctcgc gcgacggtgc cttcgtggag
     3001 ttccacaaga agtcgagcgc gtctcgaagc atcacctacg aggaggcggt cgaggaggcg
     3061 ctgtgtttcg gctggatcga ctcgaggccg aaaaagcttg acgacgatcg atgggcgctc
     3121 tggttcgcgc cgaggaagcc gaagagcgtg tggtcggcca tcaacaagga gcgtgtggaa
     3181 cggctgatcg ccgacggcgc catgaccgag gcgggtctcg cggccatcgc ggtggcgaag
     3241 gccaacggcg cctgggatca actcaacgac agcgacgcgc tcctggtccc cgacgacctc
     3301 tccgccgcgc tcgatgaaca cgtggacgcg cgccgtcatt gggacgcgtt cccgcccagc
     3361 gtccgcaaac agatcctggg gtggatcaac agcgcgaagc gcgaggacac gcgcgccaag
     3421 cgcgtcgagg aaacggcgac ctccgcggcc gagaacgtcc gcgcccatca gtggcgcccg
     3481 aaggcctagc cggcccgcga gcaccgtggg cgtaccatcg cctgcgtgtc acgtcgctcc
     3541 gactccctcg cccggccgct cctgatcttc gccgggttgg ccctcgtggc gctcacggtg
     3601 gtcgccgtca cgggcgccct ggtggttcgg aaggccgcga ccgatcaagc gctcgcggag
     3661 gccaggcagc tcaccgatct gtccgcgcgc atcgtggaac aacgcgtgaa cgacggcctg
     3721 ctgaccggtg atgccgaaac actgggcgca gtggcatcgg tcgtcttcga tgcggtgaag
     3781 acctacccga tcgtcagggt gaagatctgg gatcgcgacg gcacgatcct ctactcggat
     3841 cagaccgagt tgatcggctc gacctacgat ctcggcgagg acgaactcga ggtgatcgac
     3901 cacggcggcg tggtggccga cgtatcggac ctgagcgagc cggagaaccg gttcgagcag
     3961 gacttcggcg agttgctcga ggtgtacacg cgtatcgaca cccacaggaa gggcgagggc
     4021 acacccctgc tgttcgagac ctaccaactg gcctcgagca tcgccgaccg ccggcgtgag
     4081 ctcaccgcga cgttcctgcc gatcctggtg gtcaccctgg cggtgttcgt gctgttgatg
     4141 gtccccatcg cgtggttcct ggtccgccgg gtgcgcgcga gccagctcga acgcgaacga
     4201 ctcatgcagc gcgcactcga tgcgtccgag cgcgagcagc gccggatcgc cggcgacctc
     4261 cacgacggtc cggtgcagga gatggcgggc ctggcgatgc ggctgtccgc cgaagccgaa
     4321 ctcgtggacg acgccgacgc cggcatcacg ctccggtctt cggcctcggc ggtccgcggc
     4381 agcgtccgca tgttgcgctc ggcgatcgtg gagatctacc caccgaacct gcagcaagcc
     4441 ggcctgccgg ccgcgctctc ggatctggtg accgggctcg accggcacgg catcgaactc
     4501 tcgctggagg tcgaacccga cgccggtttc ggcgcggagg tcgacgccct gttgtaccgc
     4561 gccacgcagg aggcgctccg caacgtggag gaacatgcgg acgcgcgcca tgtgcgggtg
     4621 gtgatcacgc ggcagggcgg ccgcgcggtc ctcgaggtcg ccgacgacgg tcgcgggatc
     4681 gcgcccgacg ccctcgctga agcgccgcca cgcgggcaca tgggcctggc gatcctcggg
     4741 gacatggtga gcgacgccgg cggcacgctg acggtggaac ccggcgtcca agcgggtacc
     4801 gtcgtgcgtg tggaggtgca ggcgccgtga tcaccgtggc gatcgccgac gaccaccggg
     4861 tcgtgcgcgt cgggttggag caactcctca cgaccttcga cgacgtctcg ttcgtgggcg
     4921 ccggtgccgg cggccgggag gcggtggcga tctgcgaggc ccaccatccc gacgtgttgc
     4981 tgctcgacct gtccatgccc gacctcgacg ggatcgaagc gaccgcccag gttcgtgtgg
     5041 tctctcccga cactcgggtg gtggtgttca cctcgttctc cgatcgggac cggatcgtgc
     5101 gcgcgttgga caacggtgcg atcggctacc tgctgaagga cgcagaaccc gaggagatcc
     5161 acgccgcgat ccaggccgca tcgcgaggcg aatcgccgct cgctccacgc gccgccgccg
     5221 agctcctggc cgaccgccgg tcgcgcaccg gtgacgtgga actcaccgcg cgcgagcgcg
     5281 aggtgctggg gctggtcgtg gacggcctcg ccaacaagca gatcgcccgc cgtatgggga
     5341 tcagcgagaa gaccgtcaag ggccacctca cgaacctgtt ccagcggatc ggggtggccg
     5401 atcgcaccca ggcggcgctg tgggccgaac gcaccggcgc cttgcgcgac taggtcccgg
     5461 gaccgaagtc ccattgtgcg caccccttcg gggacgtcac catgtggtca tgcgtcggat
     5521 gctggccatc gccctcgtgt tcgccgtcct ggtcgccacc ccggcggtgg ccggcggcgg
     5581 ctccgaccgg cgtggctcgt gctcgggtgg gcccggccac tggaggctcc gggtgcaacg
     5641 ggaagatgcc gacacgttgc gggttcggtt ccggatcgag gacgtggccc cgggtcagtc
     5701 ctggcagttg ttcatctccg acaacggcac gcgcatctac tccgccacgc ggacatcgac
     5761 gtcggacggc gagatccggg tgaggaagaa cacgcgcgat cgcgccggcc gtgaccacat
     5821 cgccgccaac gcggtcaaca gcgcgaccgg cacgacctgc gaagggtccg tctcgatctg
     5881 accgggtccc gggacggtcg tcccgttgta accccgaccg gccgaccacc aagctcgacc
     5941 catcgaccgg acgagccggt ccgaccgagg agggatcaca tgatcagcaa ggtcctacgg
     6001 gtggtactcg cgtccggcct ggcgctcagc ctggccggtg ccgtgcccgc ccacgccaac
     6061 gaagacgacg tcatccgtcg cggcaactgc tccggttcca gcgattggaa gctcaagctc
     6121 tcgcccgagg acggcaggat cgaggtcgag ttcgaggtcg actcgaacgt cgtcggccag
     6181 acctggcagg tccgcctcac gaagaacggc aaccagatct tcagcggcac gcgggtgacc
     6241 cgcggagcca gtggctcctt cgagctccgc cgcgtgacct ccgacccgaa gggtgacgac
     6301 gtgttccgcg ctcacgccac ccacgcaggc gaggtgtgcg cc
//
LOCUS       DATDBZ010000414         8841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1354578, whole genome shotgun sequence.
ACCESSION   DATDBZ010000414 DATDBZ010000000
VERSION     DATDBZ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354578"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..536)
                     /locus_tag="VIX62_09890"
     CDS             complement(6..536)
                     /locus_tag="VIX62_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEY5860583.1"
                     /translation="MVAQTPSEQRRSSLVAILRDTDAPVTGTDLSGALGVSRQAVVND
                     VAILRAMGEPILGSPRGYVFGDHADGRPLSTIACKHDRATSRRETEILVDRGIAILDV
                     VVEHPLYGEVQANLFIESRSDIDRYMEILESEGSQPLSALTDGVHIHHVRAPNTDALE
                     AAKRELAEAGILLEVD"
     gene            598..1236
                     /locus_tag="VIX62_09895"
     CDS             598..1236
                     /locus_tag="VIX62_09895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEY5860584.1"
                     /translation="MDIVDPRIEAYSASLHARYDEPVVLEMEAEARASGFPIVGRNVG
                     VTLWTLAAAIGARRVFELGSGFGYSALWFAKATGPAGEIHLTDGDPENERKASDALQR
                     AGVWDVMHYHVGDALTSFAQVEGEFDIVYDDIDKDGYPDAWRAARDRVRIGGLYICDN
                     TLWYGRVPPDGEPPDGVTEAIREHNAMVAADERYVTTILPIRDGVLVATRVT"
     gene            1340..1741
                     /gene="sdhC"
                     /locus_tag="VIX62_09900"
     CDS             1340..1741
                     /gene="sdhC"
                     /locus_tag="VIX62_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY5860585.1"
                     /translation="MATRTDKKKNDDFGTVYRGHEGQWSWLFHRVTGVAIILFLFAHI
                     VDTAVIGWGPEAYNKVTAAYKNPLVHLLELGLVTAVIYHSLNGLKITLIDFFPSLAGH
                     IKALGRTTVLLFVLCMIPVVAIMGKQTLDLL"
     gene            1748..2197
                     /locus_tag="VIX62_09905"
     CDS             1748..2197
                     /locus_tag="VIX62_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HEY5860586.1"
                     /translation="MATTIDRSQPSAGAPEPQRDMRGGRERPIGGFELWAWLFMRISG
                     LVLLVLAVGHTLIMHLPSGGVDRVDFQFVAARWGSPFWRTWDWMMLTLALIHGINGLR
                     NITLDYVRKPGMRFALNMAYYVLGFTLLILGTVIVVTFDPTKWGSGA"
     gene            2197..3936
                     /gene="sdhA"
                     /locus_tag="VIX62_09910"
     CDS             2197..3936
                     /gene="sdhA"
                     /locus_tag="VIX62_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEY5860587.1"
                     /translation="MVTTHTYDALVVGAGGAGMRAAIELSDKVRTAVVSKLYPTRSHT
                     GAAQGGVCAALGNVEEDSPEWHAFDTVKGGDYLTDQPAAQLMTEEAVEAIYHLERWGL
                     PFNRTPEGRIDQRRFGGHTANHGERPVKRACYAADRTGHMILQTLYQQCVKRDVRFFN
                     EFYCLDLLRDADGATAGIVAYELATGELHVFRAKSVVMATGGYGRMFRVTSNAHALTG
                     DGPAVAWRRGVPLEDMEMFQFHPTGLAKLGVLLSEAARGEGGIVLNKDGERFMERYAP
                     TIKDLAPRDMVSRAIYQEIKEGRGAGPNHDWAYLDITHLDPKVIEEKLPDITEFARIY
                     LKVEPTKEPVPIQPTAHYAMGGIPTDLDGRVVVGPDEVPIPGLYAAGECACVSVHGAN
                     RLGTNSLLDLVVFGRRAGVDAARYAKGAELVDVPERPVADTLTVLNGFLERETGENAA
                     DIRKELQDNMFDLAFVVRSEEGLAKMQEILAGLRDRYGHVAITDKGSVYNTELMETVE
                     LGYLLDCAEALVVSARARTESRGAHYREDHPLRDDANWLKHTLAYRNDDGSIRLDFKP
                     VKMGPYVPMERKY"
     gene            4008..4709
                     /locus_tag="VIX62_09915"
     CDS             4008..4709
                     /locus_tag="VIX62_09915"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEY5860588.1"
                     /translation="MVAITVKIRRFNPEISQDSWWDEFELQADPLERLVEVLHTIKWH
                     HDGTLTFRRSCAHGICGSDAMLINGRNALACKVLVADVGAKVTIEPIRGLPVLKDLIV
                     DMEPFFDGYKTVLPFLINDSGEPEKERLQSAEERERFDDTTKCILCAACTTSCPIFWG
                     DEGYIGPAAIVNAHRFIFDSRDEAGRERLKILSEKTGVFRCRTTFNCTDACPRGIQVT
                     KAIQEVKRSILFDRF"
     gene            complement(4720..5304)
                     /locus_tag="VIX62_09920"
     CDS             complement(4720..5304)
                     /locus_tag="VIX62_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860589.1"
                     /translation="MRRGLVMLKVAGLLATALPAHASVSVQMEDFRFNPRNELLTQGA
                     QLTWFNAGTVNHTSTQDGPLKFWNTGQVASSTFSNAVTLWAAGRFTYHCNNHSIMTGI
                     VRIPVTASSATLTLGAPVTITMSSSSTFKGYTFDVQRRRDDKAWVTFKTGVGSASVES
                     TPKRVGEFRYRARIVNLGGATGGWSPATTVSVTV"
     gene            5401..6483
                     /locus_tag="VIX62_09925"
     CDS             5401..6483
                     /locus_tag="VIX62_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEY5860590.1"
                     /translation="MAHRVTIIPGDGIGPEVSAAARLVLDAADVGISWIERQAGTAAL
                     AEYGDLMPAETLDAIRSSRVAIKGPITTPVGSGFRSVNVALRQGLDLFSAVRPARALP
                     GVPIRHPSVDLVVVRENTEDLYAGIEYEEGTPEALALIQELQALSGTHVRDDAGITVK
                     PISVSGTQRIVRFAFEYARTHGRKKVTVGHKANVMRFSDGLFLQTAQIEAGNFGDIQW
                     EEIQIDHLTSRLAHDPSAFDVLLLPNLYGDIVSDLCAGLVGGLGLIPGANIGWEYAVF
                     EAVHGSAPDIAGQGLANPIAMILSGAMLLRHIGEQAAAENVEWAVDEVLRQGLVRTPD
                     LGGSSSTMEVGEAIASATSHWEHGPS"
     gene            6532..7116
                     /locus_tag="VIX62_09930"
     CDS             6532..7116
                     /locus_tag="VIX62_09930"
                     /inference="COORDINATES: protein motif:HMM:NF021079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HEY5860591.1"
                     /translation="MSEPDLDAPSAPSPRLRAAGFLCAALGALLAGGATMLTWIQPSI
                     KDLPPELTPTYLGVDLPDGLVVLGLAVVIVIAVLASRAGSTGRGRQGPAVVVIVASFI
                     VIGVSGAAVVTASSRFEPTVVDDALAIVAPDGATPQQRSDVEALVEVQVGAGPWLALA
                     GGVLALAGGIMTLAWASAASPASDPELEPDGSTD"
     gene            complement(7120..8043)
                     /gene="mdh"
                     /locus_tag="VIX62_09935"
     CDS             complement(7120..8043)
                     /gene="mdh"
                     /locus_tag="VIX62_09935"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY5860592.1"
                     /translation="MKITVVGCGFVGQTTAMRILEKGLGDVVLIDLVEAKPQGLALDL
                     KEAAPVEGYEPSIVGTNDYADTAGSDLVVITAGFPRQPGMSRMDLLGKNAAIMNSVIS
                     QVVPGSPDAIIIVVSNPLDEMTFLAAEASGFPKERVMGMAGVLDSARLRFFIAELTGA
                     SPTKVEAMTLGSHGDAMIALPRQATVDGKPLPDLVDEVTLQQLFQRTRDGGAEIVGLL
                     KTGSAYYAPSASVVAMVEAIVRDAHTTLPVCAWATGQYGIEDVYVGVPATLGRSGVEA
                     IVELDLNEDELTQLRGAAEGIRAKCADLAKL"
     gene            8158..8730
                     /locus_tag="VIX62_09940"
     CDS             8158..8730
                     /locus_tag="VIX62_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860593.1"
                     /translation="MDQPKLEVRAPLSTERLLLRPFVPGDFDAVFAMQSDPDVTRWLY
                     WGPRNEEEVRASLDLKIAATAIVAEDDPLSLAVVRKDTGALVGDLVLGWASEQHGTGE
                     VGFIVHPDHQGHGYATEAGEVLLRLAFDELGLHRVIGRTEARNGGSARVLEKLGMRQE
                     AHLVENEWVRGEWQSELVYAILDREWRARG"
BASE COUNT         1453 a         3001 c         3000 g         1387 t
ORIGIN      
        1 agccctcagt cgacttcgag caggatgccg gcctcggcga gctcgcgctt ggcggcctcg
       61 agcgcatcgg tgttgggcgc gcgcacatgg tggatgtgca ccccatccgt gagcgccgac
      121 agcggttggg acccttcgct ctcgaggatc tccatgtacc ggtcgatgtc ggaccgtgac
      181 tcgatgaaca ggttcgcctg cacctcgccg tagagcgggt gctccaccac gacgtccaag
      241 atggcgatcc cgcggtcgac caggatctcg gtctcgcggc gcgaggttgc gcggtcgtgt
      301 ttgcacgcga tcgtggacag cgggcgcccg tccgcgtggt cgccgaagac gtacccacgc
      361 ggactgccga ggatgggttc gcccatggcc cgcaggatgg cgacgtcgtt gaccacggcc
      421 tgccggctca ccccgagcgc tccggacagg tccgtgcccg tgacgggcgc gtccgtgtca
      481 cgcaggatcg cgacgaggct cgagcggcgt tgctccgaag gggtctgggc caccacggac
      541 caagtgtacg aaggagcccc gaacgcgcac acgcgcgcga cccgttacgg tggaacgatg
      601 gacatcgtcg acccacgcat cgaggcctac agcgcgtcgc tccacgcccg ctacgacgaa
      661 ccggtcgtgc tggagatgga ggcggaagcc cgcgcctccg ggttcccgat cgtcgggcgc
      721 aacgtcggcg tcacgctgtg gacgctcgcc gccgcgatcg gcgcccgtcg tgtgttcgag
      781 ctggggtcgg ggttcggcta ttcggcgttg tggttcgcga aggcgaccgg tcccgccggg
      841 gagatccatc tcaccgacgg ggacccggag aacgagcgca aggcctcgga cgccctgcaa
      901 cgcgccgggg tgtgggacgt catgcactac cacgtgggcg acgcgctgac ctcgttcgcg
      961 caggtcgagg gcgagttcga catcgtctac gacgacatcg acaaggacgg ctatcccgat
     1021 gcctggcgag ccgcccgcga ccgtgtccgc atcggcggac tctacatctg cgacaacacg
     1081 ctctggtacg gccgggtgcc tcccgacggt gagccgcccg acggcgtcac cgaggcgatc
     1141 cgcgagcaca acgcgatggt cgcggccgac gagcgctacg tcacgacgat cctgccgatc
     1201 cgcgacggcg tgctggtggc cacccgggtc acatgacctt cggacctgcg gatgccggaa
     1261 cggttacact ccgcgccgtc cgatggacgg ttcggttgac ggcttcgccg gaccgcgagc
     1321 tacgctgaag gcgataaccg tggcgacccg aaccgacaag aagaagaacg acgacttcgg
     1381 caccgtgtac cgcggccacg aaggccagtg gtcctggctc ttccaccggg tcacgggcgt
     1441 cgcgatcatc ctgttcctgt tcgcgcacat cgtcgacacc gctgtgatcg gctggggacc
     1501 cgaggcctac aacaaggtca ccgcggccta caagaacccg ctggtgcacc tgctggaact
     1561 cgggctggtg accgcggtga tctaccactc gctcaacggc ctgaagatca cgctgatcga
     1621 cttcttcccg tccctggcag gccacatcaa ggcgctcggt cgcaccaccg tgctgctgtt
     1681 cgtgctctgc atgatcccgg tggtcgcgat catgggcaag cagaccctgg acctgttgta
     1741 ggagcccatg gccaccacga tcgatcgttc gcagccgtcc gccggagccc ccgagcccca
     1801 gcgcgacatg cgtggtggac gcgaacgtcc gatcggcggg ttcgagctct gggcctggct
     1861 cttcatgcgg atcagcggcc tggtgctgct ggtcctggcc gtgggccaca cgctgatcat
     1921 gcacctgccc agcggcgggg tggaccgggt ggacttccag ttcgtcgcag cccgatgggg
     1981 aagcccgttc tggcgcacct gggactggat gatgctcacg ctggccctga tccacggcat
     2041 caacggcctg cgcaacatca cgttggacta cgtgcgcaag cccgggatgc ggttcgcgct
     2101 caacatggcc tactacgtgc tgggattcac gctgttgatc ctgggcacgg tcatcgtcgt
     2161 gacgttcgac cccacgaagt ggggatcggg cgcctgatgg tcaccacgca cacctacgac
     2221 gcgctggtcg tgggcgccgg cggcgccggg atgcgcgccg cgatcgaact cagcgacaag
     2281 gtccgcaccg ccgtcgtctc caagctctac cccacccgct cgcacaccgg tgccgcgcag
     2341 ggcggggtgt gcgccgccct gggcaacgtc gaggaggaca gccccgagtg gcacgcgttc
     2401 gacaccgtca agggcggtga ctacctcacc gatcagcccg ccgcgcaact gatgaccgaa
     2461 gaggccgtcg aggcgatcta ccacctcgaa cgctgggggc tgccgttcaa ccgcacaccc
     2521 gaaggcagga tcgaccaacg gcggttcggc ggccataccg cgaaccacgg tgagcggccc
     2581 gtgaagcgcg catgctatgc cgccgaccgc accggccaca tgatcctgca gacgctctat
     2641 cagcagtgcg tgaaacgcga cgttcgcttc ttcaacgagt tctactgcct cgacctgctg
     2701 cgggacgccg acggcgccac cgccgggatc gtggcgtacg agctcgccac cggtgagctc
     2761 catgtgttcc gggccaagag cgtcgtgatg gccaccggcg gctatgggcg catgttccgc
     2821 gtcacctcga acgcccatgc cctgaccggc gacggtcccg cggtggcgtg gcggcgcggc
     2881 gtgccgctcg aggacatgga gatgttccag ttccatccga ccggcctcgc caagctcggc
     2941 gtgctcttgt ccgaggccgc acgcggcgaa ggcggcatcg tgctcaacaa ggacggcgag
     3001 cgattcatgg agcgctacgc ccccacgatc aaggacctcg ctcctcgcga catggtgagc
     3061 cgcgcgatct accaggagat caaggagggt cgcggcgccg gccccaacca cgactgggcc
     3121 tacctcgaca tcacgcacct cgaccccaag gtgatcgagg agaagctgcc cgacatcacc
     3181 gagtttgccc gcatctacct gaaggtggag cccacgaagg agccggtgcc gatccagccc
     3241 accgcgcact acgcgatggg cggcatcccg accgacctcg acggccgtgt cgtggtcggc
     3301 cccgacgagg tgccgatccc cggcctgtac gccgccggcg aatgcgcctg tgtcagtgtg
     3361 cacggcgcga accgactggg caccaactcg ctgctcgacc tcgtggtgtt cggtcgccgc
     3421 gccggcgtcg acgccgcgcg ctacgccaag ggggccgagc tggtcgacgt gcccgagcgt
     3481 ccggtcgccg acacgctcac cgtgctgaac ggcttcctgg agcgcgagac cggcgagaac
     3541 gccgccgaca tccgcaagga gctccaggac aacatgttcg acctggcgtt cgtggtccgc
     3601 agcgaggagg gcctggcgaa gatgcaggag atcctggctg ggctgcgcga tcgctacggc
     3661 catgtcgcga tcacggacaa aggcagcgtg tacaacacgg agctgatgga gacggtggaa
     3721 ctcggctact tgctcgactg cgccgaagcc ttggtggtca gcgcccgcgc tcggacggag
     3781 agccgcggcg cccactatcg ggaggaccac cccctgcgcg acgacgccaa ctggctcaag
     3841 cacacgctgg cctaccgcaa cgacgacggg tcgatccgtc tggacttcaa gccggtgaag
     3901 atgggtccct acgtccccat ggagaggaag tactgatggc cagctcggcc gaagtgctgc
     3961 aaggatcgga cgcccgcgag atccgtgctc ggaacgatga ccacgcgatg gtggccatca
     4021 ccgtgaagat ccgccggttc aacccggaga tctcgcagga ttcgtggtgg gacgagttcg
     4081 agctacaggc cgacccgctg gagcggctgg tggaggtgct gcacaccatc aagtggcacc
     4141 acgacggcac cctgacgttc cgccggtcgt gcgcgcacgg gatctgtgga tcggacgcca
     4201 tgctgatcaa cggtcgcaac gccctcgcgt gcaaggtgct ggtggccgac gtcggcgcca
     4261 aggtcacgat cgaaccgatc cgcgggctcc cggtcctcaa ggacctcatc gtcgacatgg
     4321 agccgttctt cgacggctac aagacggtgc tgccgttcct gatcaacgac tccggtgagc
     4381 ccgagaagga acgcctccaa tcggccgagg agcgcgaacg gttcgacgac accaccaagt
     4441 gcatcctgtg cgcggcgtgc accacgtcgt gtccgatctt ctggggcgac gagggctaca
     4501 tcgggcccgc tgccatcgtg aacgcgcacc ggttcatctt cgactcgcgt gacgaggccg
     4561 gccgcgagcg gctgaagatc ctgagcgaga agaccggcgt gttccggtgc cggaccacgt
     4621 tcaactgcac cgatgcctgc ccgcgaggca tccaggtgac gaaggcgatc caggaagtga
     4681 agcgctcgat cctgttcgat cggttctagc ggaaccgcat cagaccgtca ccgacaccgt
     4741 cgtcgcgggg gaccatccac ccgtggctcc acccaggttc acgatgcgtg cccgataccg
     4801 gaactcgccg acgcgcttcg gtgtgctctc gacgctcgcc gagcccacgc cggtcttgaa
     4861 cgtgacccag gccttgtcgt cgcggcggcg ctgcacgtcg aacgtgtagc ccttgaacgt
     4921 ggaggaggac gacatcgtga tcgtcaccgg cgctcccagg gtgagcgtgg ccgaggaggc
     4981 cgtgaccggg atgcggacga tccccgtcat gatcgagtgg ttgttgcagt ggtaggtgaa
     5041 ccttcccgca gcccacaggg tgacggcgtt gctgaacgtc gacgacgcca cctggcccgt
     5101 gttccagaac ttcaagggtc cgtcctgcgt ggacgtgtgg ttgaccgtgc cggcgttgaa
     5161 ccaggtgagc tgtgcccctt gcgtcagcag ctcgttcctc gggttgaagc ggaagtcctc
     5221 catctgcacg ctcacggagg cgtgcgccgg caacgcggtc gccaggagcc ccgcgacctt
     5281 caacatcacc aagcctcgtc gcatcgttcc tccccaagca cgagaaccgg aacggcaccg
     5341 taccccaacg acgcccaacc cgcgaggggg ccggtcggcg ttcggtacag tgccgactcg
     5401 atggcacacc gagtgacgat catcccgggc gacggcatcg gccccgaggt ctcggcggcc
     5461 gccagactcg tcctggacgc cgccgacgtc ggcatctcgt ggatcgaacg ccaggccggc
     5521 accgcggccc tcgccgagta cggcgacctc atgcccgcag agacgctcga tgcgatccgg
     5581 tcgagccgcg tcgcgatcaa gggtccgatc accacgccgg tggggagcgg cttccgcagc
     5641 gtgaacgtcg ccctgcggca ggggctcgat ctgttcagcg ccgtgcgacc cgcccgagca
     5701 ctccccgggg tgccgatccg tcatccgagt gtcgacctgg tggtcgtgcg tgagaacacc
     5761 gaggacctgt acgcggggat cgagtacgag gagggcacgc cggaagccct cgccttgatc
     5821 caggagttgc aggcactcag cggcacccac gtgcgcgacg acgcgggcat cacggtcaag
     5881 cccatcagcg tcagcgggac gcagcgcatc gtgcggttcg cgttcgagta cgcgcgcacg
     5941 cacggccgca agaaggtcac ggtgggacac aaggccaacg tgatgcgctt ctccgacggg
     6001 ttgttcctgc agaccgctca gatcgaagcg ggcaacttcg gcgacatcca gtgggaggag
     6061 atccagatcg accacctcac cagccggctg gcgcatgatc ccagcgcctt cgacgtgctg
     6121 ctcctgccca acctgtacgg cgacatcgtg tccgacctgt gcgcagggct ggtcggtggg
     6181 ctcggcttga tccccggcgc caacatcggg tgggagtacg ccgtgttcga ggccgtgcac
     6241 ggcagcgcgc ccgacatcgc cggccagggc ctcgcgaacc ccatcgccat gatcctgtca
     6301 ggggcgatgc tgctccgcca catcggcgag caggccgccg ccgagaacgt ggagtgggcg
     6361 gtcgacgagg tgctccgcca agggctcgtc cgcacccccg acctcggggg ctcctcgagc
     6421 acgatggagg tcggcgaggc gatcgcgtcc gccacgagcc attgggagca cggccccagc
     6481 tgacggtaca gcttgcttgg aaccgggttc gaggggtgcc acactcgccg aatgtccgag
     6541 cctgacctcg atgctccctc ggccccctct ccacgtctcc gcgcggcagg gttcctgtgt
     6601 gcggcgctcg gcgcgctgct cgccggcggc gccacgatgc tcacgtggat ccagccgtcc
     6661 atcaaggatc tgccgcccga actcaccccc acctacctag gggtcgatct gcccgacggc
     6721 ctcgtcgtgc tgggcttggc cgtcgtgatc gtgatcgcgg tgctcgcgtc gcgggccggc
     6781 agcacggggc gcggccgcca aggccctgcg gtcgtggtga tcgtcgcctc cttcatcgtg
     6841 atcggcgtgt cgggtgccgc ggtcgtgacc gcgtcgagtc ggttcgaacc caccgtggtc
     6901 gatgacgctc tggccatcgt ggctccggat ggcgccacac cccagcaacg ctccgacgtc
     6961 gaggcgctgg tcgaggtgca ggtgggcgca ggaccctggt tggcgctggc gggcggggtc
     7021 ctggcgctgg cgggcggcat catgacgctg gcctgggcga gcgccgccag tccggcttcg
     7081 gatccggagc tggagcccga cggttcgacg gactgatcgc tacagcttcg cgaggtccgc
     7141 gcacttggca cggatgccct cggccgctcc ccggagctgc gtcagctcgt cctcgttgag
     7201 atccagctcc acgatcgctt ccacgcccga gcgccccagg gtggcgggca cgccgacgta
     7261 gacatcctcg atcccgtact gaccggtcgc ccatgcgcag acagggagcg tggtgtgcgc
     7321 atcccgaacg atggcctcga ccatggccac gaccgaagcg ctgggtgcgt agtaggcgga
     7381 tccggtcttg aggagcccca cgatctcggc gcccccgtcg cgggttcgct ggaagagttg
     7441 ctgcagggtg acctcgtcga cgaggtcggg cagaggtttg ccgtcgacgg tggcctggcg
     7501 cggcagggcg atcatcgcgt cgccgtgtga gcccagggtc atcgcctcga ccttcgtggg
     7561 ggatgccccg gtgagctcgg cgatgaagaa ccgcaggcgt gctgagtcga gcacccctgc
     7621 catccccatg acccgctcct tcgggaagcc ggaggcttcg gcggccagga aggtcatctc
     7681 atcgagcggg ttcgacacca cgatgatgat cgcgtcgggc gagccgggca cgacctggga
     7741 gatcaccgag ttcatgatgg cggcgttctt gccgagcaga tccatgcggc tcatgccggg
     7801 ctgacgcggg aacccggcgg tgatcacgac gaggtcgctc ccggcggtgt cggcgtagtc
     7861 gttcgtgccg acgatcgagg gctcgtagcc ctcgaccgga gcggcttcct tgaggtcgag
     7921 cgcgagccct tgcggcttgg cttcgacgag gtcgatcagg acgacgtccc cgaggccctt
     7981 ctccaggatg cgcatggcgg tcgtctgccc gacgaacccg caccccacga cggtgatctt
     8041 catgacggtg aactccttca cggttgatcg gcacagtcgg caccgttgat cgactcgaac
     8101 gcctcaggct atcgaacgcg tgacgggacc gacggtcccg cacgtaccat caacggcgtg
     8161 gatcagccga agctcgaggt gcgcgcccca ctgagcaccg aacgcctgct cttgcgaccc
     8221 ttcgtcccgg gggacttcga cgcggtgttc gcgatgcagt cggaccccga cgtgacgcgg
     8281 tggctgtact ggggcccgcg gaacgaagag gaggtccggg catcgctcga cctgaagatc
     8341 gcggccaccg ccatcgtcgc cgaggatgac ccactgtccc tggccgtggt tcgcaaggac
     8401 accggcgccc tcgtcggcga cctcgtgctg gggtgggcca gtgagcaaca cggtacgggt
     8461 gaggtcgggt tcatcgtgca tccggaccat cagggccacg gctacgcgac ggaggcgggc
     8521 gaggtgttgc tgcgcctggc cttcgacgaa ctcggattgc accgcgtgat cggccggacc
     8581 gaggcgcgca acgggggttc ggcccgcgtg ctggagaagc tggggatgcg gcaggaagcg
     8641 cacctggtcg agaacgaatg ggtcaggggc gaatggcaga gcgaactcgt ctacgcgatc
     8701 ctggaccgcg aatggcgggc ccgcggctga gcgcgcacgc ggctgtgcgt cagcggcgtg
     8761 agccgcggcc catcacggcg taggcgagcg ctccgaggaa cccgaggagc acgatcaaca
     8821 acacccacat caccttggtc c
//
LOCUS       DATDBZ010000415         4692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1354823, whole genome shotgun sequence.
ACCESSION   DATDBZ010000415 DATDBZ010000000
VERSION     DATDBZ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354823"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VIX62_09945"
     CDS             complement(<1..1160)
                     /locus_tag="VIX62_09945"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEY5860594.1"
                     /translation="MRVPSDLPPPRRERTSSRRPVRRARVAILLAVTVLVGLAFSASR
                     VADAYVDALWYRSVGQSDVFWEVIRTELVLAIGFSAGFAIFALTNLVIADRLAPLITP
                     DGTEEIRDRYRAVVGTHHRIARAAVAVGFGLIAGLPTAGRWQEWMLFRNATRFGTRDR
                     QFDTDVGFYVFRLPFLSFLVSWLFASFVIVTLLTAIAHYLNGGIRVQVAGRRVTSHVK
                     LHLSALLGVLALLKAADYWLQRFELTTSQRGYIGGAGFTDIEASLPVLQLLILIAVLA
                     AVLFVANVRQQGFRLPVMAIGLWSVVAVVAGAIYPTVVQRFQVEPNQSTREAPYIVRN
                     ILATRLAMNLDEVEVVPISVGAVSGADVEAGVDAFADTRLLDPQIIGPTYQV"
     gene            complement(1202..1747)
                     /locus_tag="VIX62_09950"
     CDS             complement(1202..1747)
                     /locus_tag="VIX62_09950"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEY5860595.1"
                     /translation="MAIYVALLRAINVGSGRGVKMTDLARVVSEAGGEQVSTYIQSGN
                     VVFAHDTGSPPELEAELENRLATIASFHVSVIVRTAQEWDVVISECPYDVEDPTKLHV
                     TFSKGTLPDATLDAFDQAPFAPASFGWRKREIYLYLPDGIGRAKLPIALGKLDTTASP
                     TTTRNWRTVLELQKYARRLAT"
     gene            complement(1972..2412)
                     /locus_tag="VIX62_09955"
     CDS             complement(1972..2412)
                     /locus_tag="VIX62_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860596.1"
                     /translation="MSAPVVEIAQEATSQLVDAFARLIPQLSKSSPPPTRDELDEMIT
                     SPASDVLIAKEGDDILGALTLIVFRIPTGVRAWIEDVVVDDAARGKGVGDLLNRFALQ
                     LAASKGAKTVDLTSRPSRDAANRLYQRIGFEPRDTNVYRFELEG"
     gene            2486..3394
                     /locus_tag="VIX62_09960"
     CDS             2486..3394
                     /locus_tag="VIX62_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860597.1"
                     /translation="MDDATLSATARKLGSLLEPVTGQVYFSPECHAEYVALGFDPSPS
                     VRNGVALPDGPAYFTSRGSVMGQVTGEVVAAAFAVFNPAVVVPCVELGWTRTDASTIS
                     AARDNGAIGQLQRILGDEPVGLARVIEILNRMVEPLRPEGRPLFAGLRSLGLPADRIG
                     AMWRLGDMLREYRGDSHTASWISAGIDATEIGLMSELYWGLSPRSYSRTRAWTADDFD
                     AATERLQQRGILDDAAELTQDGRRLREDVEVATDRQMRATIHAGGDDLDELFTLLAPW
                     GQAIRAAGGYLATGPHDLARAAGGRG"
     gene            3404..4486
                     /locus_tag="VIX62_09965"
     CDS             3404..4486
                     /locus_tag="VIX62_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255838035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3749..3751,aa:Sec)
                     /transl_table=11
                     /product="ResA-like WAxxUGC motif-containing protein"
                     /protein_id="HEY5860598.1"
                     /translation="MLLESLTADPIEFADRTGWDAKPEGLCRGEICVPAPGARRDDGS
                     IDVSVAAARLGMPLVHDELHGLWALGPATATGTALSTAVAADPTLVDRDGIDFRLSSL
                     HGRKVLLVAWASYUGCRTSLPGWQALHLELEPKGLTVVTVALDVDPAHAHSWIDAASP
                     THPSVIDTTHATGELFGFVNIPMAVWIDESGTLVRPAESASIERSPLRDMDIPEGLPE
                     RMVRMLTEVKNIPDDADAYRGAIVDWVERGSESRFALRPDEVVARSQPRGDDEARAAA
                     CFELGEHLRRTIGPDAAVPWWREAHRLFPGNWTYKRQAWTLVTTPAGAGANDLIQDAN
                     DVYEGNWLDDVIAGGGGAAYTVRPQL"
     gene            4497..>4692
                     /locus_tag="VIX62_09970"
     CDS             4497..>4692
                     /locus_tag="VIX62_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY5860599.1"
                     /translation="MEHVRWHSDLELRQPVMVAGFTGWNDAADAASTAVRVLIEGWGA
                     TLVASIDPEEFTDFSTIRPHV"
BASE COUNT          780 a         1642 c         1547 g          723 t
ORIGIN      
        1 tcaacctgat acgtcggccc aatgatctgc ggatccaaca accgcgtatc cgcaaacgca
       61 tccacccccg cctccacatc cgcccccgac accgcaccaa ccgaaatcgg caccacctca
      121 acctcatcca aattcatcgc caacctcgtg gcgagaatgt tgcggacgat gtacggcgcc
      181 tcacgcgtcg actgattggg ctcgacctgg aaccgctgca cgaccgtcgg atagatcgcc
      241 cccgccacca ccgccaccac actccacagc ccgatcgcca tcaccggcaa ccggaaaccc
      301 tgctgacgga cgttcgccac gaagagcacc gcggcaagca cggcgatgag gatcagcaac
      361 tgcagcaccg gcagggacgc ttcgatgtcg gtgaagccgg caccgccgat gtagccccgc
      421 tgcgacgtcg tcagctcgaa ccgttgcagc cagtagtccg ctgccttcaa cagcgccagc
      481 acgcccaaca gggccgagag gtggagcttg acgtgcgatg tgacccgccg ccccgccacc
      541 tggacccgga tgccgccgtt caggtagtgg gcgatggcag tcagcaacgt cacgatcacg
      601 aacgacgcga ataaccagct gaccaggaag ctcaagaacg gcaaccggaa cacatagaac
      661 ccgacatcgg tgtcgaactg ccgatccctc gtcccgaatc tcgtcgcatt gcggaacaac
      721 atccactcct gccaccgccc cgccgtcggc agaccggcga tcagcccgaa cccgactgcg
      781 accgcggccc gggcgatccg gtggtgcgtg cccaccaccg cccggtagcg gtctcggatc
      841 tcctccgtgc cgtcgggagt gatgagcggc gcgagccggt ctgcgatcac gaggttcgtg
      901 agcgcgaaga tcgcaaaacc ggcgctgaag ccgatcgcca ggacgagctc ggtgcggatc
      961 acctcccaga acacgtccga ctggcccacc gaccggtacc agagggcgtc gacgtacgca
     1021 tcggcgactc gacttgccga gaatgcgagc ccgacgagga ccgtgaccgc cagcaggatc
     1081 gcaacgcggg cacgacgaac gggtcgccgg ctcgaggtcc gctcgcgccg cggcggcgga
     1141 aggtcggacg gaaccctcat gcatgcacgc tagccggggg gcctgtcgcc gccccagcag
     1201 gtcaggtagc gaggcggcgg gcgtacttct gcagctcgag cacggtgcgc cagttccggg
     1261 tggtcgtcgg cgaagcggtc gtgtcgagtt tcccgagcgc gatcggcagc ttggcgcggc
     1321 cgatgccgtc gggcaggtac aggtagatct cacgcttgcg ccagccgaac gatgccgggg
     1381 cgaacggggc ctggtcgaac gcatcgagcg tggcgtccgg cagcgtgccc ttcgaaaatg
     1441 tgacgtgcag cttcgtcgga tcctcgacgt cgtacgggca ttcgctgatc acgacgtccc
     1501 actcctgcgc cgtgcggaca atgactgaga cgtggaagct ggcgatcgtt gccagtcggt
     1561 tttcgagctc cgcttccaac tcaggaggcg agcccgtgtc gtgggcgaac accacgttgc
     1621 cactctggat gtaggtgctc acctgctcgc cccctgcttc gctgacgacg cgcgccaggt
     1681 cggtcatctt cacgcctcga cccgaaccga cgttgatggc acggagcagc gcgacgtaga
     1741 tggccatctc cattccgtac cacacgattc gggcctccgg ctgaccgatg tgcagccgtc
     1801 ggaggcctcg gcccggacgc tcggtccgca tgacaccgtt tgcgcgcttg gttgcgcaga
     1861 gcgccactcg gcgcgcaaac gcccgaggag ctcagcaggg tttgcgcgct tggttgcggt
     1921 attcgccacc cggcgcgcaa acgcccgcag gctcggcggg actcgtgttc gtcagccctc
     1981 gagctcgaac cggtacacgt tggtgtcacg cggctcgaac ccgatgcgtt ggtagaggcg
     2041 gttcgccgca tcccgtgacg ggcgcgacgt gaggtcgacc gtcttcgcgc ccttcgacgc
     2101 cgccagctgc aacgcgaagc gattcagcag gtcacccaca cccttgcccc gcgccgcatc
     2161 gtcgacgacg acgtcctcga tccaggcccg aacgcccgta gggatgcgga acacgatcag
     2221 cgtcagggcg ccgaggatgt cgtcaccctc cttcgcgatc aacacgtctg aggcggggga
     2281 cgtgatcatc tcgtcgagct cgtcgcgggt cggcggcggt gagctcttcg acagctgcgg
     2341 gatgagacgg gcgaacgcgt ccacgagctg ggacgtggcc tcttgggcga tctcgacgac
     2401 tggtgcgctc acggaacggg caccgtagcg aagaccgggc ggcacggctc gtcgcgcctg
     2461 ccaccgccag gcctaggttg cggcgatgga cgacgcaact ctttcggcga ccgctcgcaa
     2521 actcggatcg ctgctcgagc cggtcaccgg tcaggtgtac ttctcacccg agtgccacgc
     2581 ggagtacgtc gcgctcggct tcgacccgag cccctccgtc aggaacggcg tcgcccttcc
     2641 cgacgggccg gcctacttca cgagccgtgg ctcggtgatg gggcaggtaa ccggtgaggt
     2701 cgtcgccgcc gcgttcgcgg tgttcaaccc tgctgtagtc gtcccgtgcg tcgagctcgg
     2761 ctggacgcga acagacgcct cgaccatctc ggccgctcgc gacaacggcg ccatcggcca
     2821 gctgcagcgc atcctggggg acgagccggt ggggctggcc cgtgtcatcg agatcctgaa
     2881 ccggatggtc gagccactgc ggccggaagg ccgaccactc ttcgccggac tccgctcgct
     2941 ggggctgccc gccgatcgca tcggcgcgat gtggcgcctc ggcgacatgc tccgggagta
     3001 ccgcggcgac tcgcacacgg cctcatggat ctcggcgggc atcgacgcga cggagatcgg
     3061 cctgatgtca gagctctact ggggtctgtc accacgctcg tacagccgta cccgggcgtg
     3121 gacggccgac gatttcgatg ccgcgaccga gcgactgcag caacggggga tcctcgacga
     3181 tgccgccgag ctcactcagg acggacgccg gctgcgcgag gacgtcgagg tggcgaccga
     3241 tcgccagatg cgggccacca tccacgccgg cggtgacgat ctcgacgagc tgttcacgct
     3301 gctggcaccg tggggtcagg cgatccgcgc cgcgggcggg tatctcgcca ccggcccgca
     3361 cgacctcgcc agagcggccg gcgggcgcgg atagggtcgc ctggtgctcc tcgagtcgct
     3421 caccgccgat ccaatcgagt tcgccgaccg caccggttgg gatgccaaac ccgaaggtct
     3481 gtgccgcggg gagatctgcg tcccggcgcc cggagcacgg cgcgacgacg gctcgatcga
     3541 cgtctcggtg gcggccgctc gcctggggat gcccctggtg cacgacgagc tccatggcct
     3601 gtgggcgctc ggcccggcca ccgcgaccgg caccgcgctg tccaccgccg tcgccgccga
     3661 cccgaccctc gtcgatcgtg acggcatcga tttccggctc tcctcgctgc acgggcgcaa
     3721 ggtgctgctc gttgcgtggg cgagttattg aggctgtcgc accagcctgc ccgggtggca
     3781 ggcgctccac ctcgagctcg agccgaaggg cctgaccgtc gtcacggtgg ccctggacgt
     3841 cgatccggcc cacgcccact cgtggatcga cgccgcatcg ccgacccatc cgagtgtgat
     3901 cgacaccacc cacgccacgg gtgagctgtt cggattcgtc aacatcccga tggcggtgtg
     3961 gatcgacgaa tcagggaccc tcgtgcgccc ggcggaatcc gccagcatcg agcgcagccc
     4021 gttgcgtgac atggacatcc ccgagggatt gccggagcgc atggtccgga tgttgaccga
     4081 ggtcaagaac atccccgacg acgctgatgc gtaccggggc gccatcgtcg actgggtcga
     4141 gcgcggtagc gagagccgtt tcgcgctgcg tcccgacgag gtcgtggcgc gatcacaacc
     4201 acgcggcgac gatgaggctc gcgccgccgc ctgcttcgag ctcggcgagc accttcgacg
     4261 cacgatcggg ccggacgcgg cagtgccgtg gtggcgtgag gcccaccggt tgttccctgg
     4321 caactggacg tacaagcgcc aggcctggac gctcgtcacg acgcctgccg gtgccggcgc
     4381 caacgatctg atccaggacg ccaacgacgt ctacgagggc aactggctcg atgatgtcat
     4441 cgctggggga ggcggcgccg cgtatacggt gcgccctcag ctgtagcgtt tcacgggtgg
     4501 aacacgtccg ttggcacagt gatctggagc tgcgtcagcc ggtgatggtg gccggcttca
     4561 ccggctggaa cgacgctgcc gacgctgcct ccaccgccgt gcgcgtgctg atcgaggggt
     4621 ggggcgcgac cctcgttgcc agcatcgacc ccgaggagtt cacggacttc tccacgatcc
     4681 gcccgcatgt cc
//
LOCUS       DATDBZ010000416         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1354922, whole genome shotgun sequence.
ACCESSION   DATDBZ010000416 DATDBZ010000000
VERSION     DATDBZ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354922"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VIX62_09975"
     CDS             complement(<1..786)
                     /locus_tag="VIX62_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY5860600.1"
                     /translation="MTNDVRDMSLAPGGRDRIEWADAEMPVTASIRERFEKEKPLDGI
                     RIGACLHVTTETANLMLTLQAGGAQVALCASNPLSTQDDVAAALVDAGIDTYAIRGED
                     TDTFFKHINAVLDIRPQITMDDGADMVSVLHKERADQIAEIIGGTEETTTGVIRLRAM
                     AKDGALKYPIVSVNDADTKHLFDNRFGTGQSTIDAIMRSTNRLLAGRTFVVCGYGMCG
                     RGVASRARGMGAHVVVTEVDPTAALEAVMEGYRVMPLRDAARVG"
     gene            complement(812..1645)
                     /locus_tag="VIX62_09980"
     CDS             complement(812..1645)
                     /locus_tag="VIX62_09980"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY5860601.1"
                     /translation="MTHGVPGPGDDLPARGAAVVRASTDLVPRVGIVLGSGLGPALGP
                     DLQEEASFAFTDLPGFPPPGVPGHEGRLVVGRLAGVPVAAFFGRVHFYEGHGMDVPAL
                     LPRLARELGADTMVVTAAVGGLVPGEPAGTVVVLRDHLNMMGSAPMRAWRYPDGMPPF
                     ISTEEVYDPILRERASERAQALGIGSSMGVYAAMSGPAFETPTEVRFLQQAGATVVGM
                     SMVPEALPAHAMGMRVLGLCSLTNAFGEHVEHEDVVRVSNETAIAVGRLLVDLVPRLD
                     G"
     gene            complement(1642..>2476)
                     /locus_tag="VIX62_09985"
     CDS             complement(1642..>2476)
                     /locus_tag="VIX62_09985"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEY5860602.1"
                     /translation="DVNRSPELPEFAGPHTLVVTCSYSGGTAETLEAFREAQRRGCRI
                     IALTGGGELADRAESAGAGLTVIPSGYVPRAAFGYLVFGLLGTLEAAGLLPPLATDVD
                     ETIEELERLVIALGPDRPRDEKNAAKHLAWNLGDRRPVVWGADGIGAVAAARWKAQWN
                     ENAKVPAWWAALPELDHNEVVGWAEGEGNDSFVIALRHEGEHPDVALRFEPSLEIARG
                     SGAVTEEVWAAGRSPLARLCSLVITGDYASVYLALMRGVDPSPMDAIDHLKAVLAEAS
                     S"
BASE COUNT          406 a          897 c          777 g          396 t
ORIGIN      
        1 gcccacacgc gcggcgtcgc ggagcggcat gacgcgatac ccctccatca cggcttcgag
       61 cgcggcggtc ggatcgacct cggtgaccac cacgtgtgcg cccatgcccc gcgccctgga
      121 tgcgacacct cgcccgcaca tgccgtaacc gcacaccacg aacgttcgcc cggccaacaa
      181 gcggttggtg gaacgcatga tggcatcgat cgtcgactgc cccgtgccga aacggttgtc
      241 gaacaggtgc ttggtgtccg cgtcgttcac gctgacgatc gggtacttca gggccccgtc
      301 cttcgccatc gcgcgcaggc ggatcacccc ggtcgtggtc tcctcggtgc cgccgatgat
      361 ctcggcgatc tggtcggccc gctccttgtg caggacgctc accatgtcgg ccccgtcatc
      421 catcgtgatc tggggacgga tgtcgagcac cgcgttgatg tgcttgaaga acgtatcggt
      481 gtcctcgccc ctgatcgcgt aggtgtcgat accggcgtcg accagtgccg ccgccacgtc
      541 atcctgtgtc gacaaggggt tcgaggcgca caaggcgacc tgtgcacccc ccgcctgcag
      601 cgtcagcatc aggttcgcgg tctcggtggt cacgtgcaga catgcaccga tgcggatccc
      661 gtccagcggc ttctctttct cgaaccgttc ccggatcgaa gccgtcaccg gcatctcggc
      721 gtcggcccat tcgatgcggt cccgcccgcc cggcgccaag ctcatgtcac gtacgtcgtt
      781 ggtcatgcgt cgtctccttc ggcgtccctg gtcatccgtc caggcgcggg accaggtcga
      841 ccagcaaccg tcccacagcg atggcggtct cgttcgatac ccgtaccacg tcttcgtgtt
      901 ccacatgctc gccgaacgcg ttcgtcagcg agcacaaccc cagcacgcgc atgcccatgg
      961 cgtgtgcggg aagcgcttcg ggcaccatgg acatgcccac gacggtcgca ccggcctgct
     1021 gcaggaaacg cacctctgtc ggcgtctcaa aggccggccc gctcatcgcg gcatagacac
     1081 ccatggacga tccgatgccc agcgcctgcg ctctctccga tgcccgctca cggaggatcg
     1141 gatcgtagac ctcctcggtg ctgatgaacg gcggcatgcc gtcgggatag cgccacgctc
     1201 gcatcggggc ggatcccatc atgttgaggt gatcacggag caccaccaca gtgccggcag
     1261 gctctcccgg caccaggcca ccgacggcag cggtcacgac catggtgtcg gcgcccagtt
     1321 cccgtgccaa gcgaggcagc aatgccggca cgtccatacc gtggccctca tagaagtgca
     1381 cccggccgaa gaaggccgcg accggcacgc cggccagccg ccccacgact agccgtccct
     1441 catgtcccgg gacacccgga ggcgggaagc caggaaggtc ggtgaacgcg aagcttgcct
     1501 cttcctgaag atcgggcccc aatgccggac cgaggccgga acccagcacg atcccgacgc
     1561 gaggcaccag gtccgtgctg gcccggacga ccgcagcacc ccgggccggc aggtcgtcgc
     1621 cgggtccggg cacaccatgg gtcacgagga cgcctccgcg agcacagcct tgagatgatc
     1681 gatcgcgtcc atcggggatg ggtcgacgcc gcgcatcagg gcgaggtaga cgctggcgta
     1741 gtcaccggtg atcaccaagg aacagagtcg agcgagcggc gaacgtcccg cggcccagac
     1801 ctcctcggtc acggcacccg aaccccgcgc gatctccagc gacggctcga accgcaacgc
     1861 cacgtccggg tgctcccctt cgtgacgcag cgcgatcacg aacgaatcgt tgccctcacc
     1921 ctcagcccag cccacgactt cgttgtggtc gagctcgggc agggctgccc accacgccgg
     1981 gaccttggcg ttctcgttcc actgcgcctt ccatcgggcg gccgcgacgg caccgatgcc
     2041 gtcggcgccc cacacgacag ggcgacggtc gccgaggttc cacgcgaggt gtttcgctgc
     2101 gttcttctcg tccctaggtc gatcgggtcc gagggcgatg accaaccgtt cgagctcctc
     2161 gatcgtctcg tcgacatcgg tcgccagggg tggaagcaga cccgccgctt ccagagtgcc
     2221 gaggagcccg aagaccaggt agccgaatgc cgcgcggggc acgtagccgc tggggatcac
     2281 ggtgagaccc gcgcccgctg actcggcacg atcggcgagt tcgccacccc ccgtgagcgc
     2341 gatgatccgg cagcctcgcc gttgggcctc ccggaacgcc tcgagtgttt cagccgttcc
     2401 gccgctgtac gaacacgtga cgacgagggt gtgcggtccc gcgaactccg gcagctcagg
     2461 gcttcggttc acatcg
//
LOCUS       DATDBZ010000417         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_540843, whole genome shotgun sequence.
ACCESSION   DATDBZ010000417 DATDBZ010000000
VERSION     DATDBZ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540843"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VIX62_09990"
     CDS             <1..369
                     /locus_tag="VIX62_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5860603.1"
                     /translation="NDPKVQPAVREKMLGLGRPELDAFRLAAEAGVKVAMGTDCPVAP
                     HGTNLNELRHMAANGFTPAQSLHAATKSAAELMGLQDELGTLEPGKRADVVVVDGDPL
                     DFQDYESRIHQVWKDGVRVS"
     gene            379..1218
                     /locus_tag="VIX62_09995"
     CDS             379..1218
                     /locus_tag="VIX62_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5860604.1"
                     /translation="MVGIVLALVGAAGWGTADFLGGLSTKKLSIWVVSVVSQIAGLVF
                     MAVVVVSLGRGPVEMKVVWLGMAAGVCGLIGLSALYTALALGPMGVVAPISALSVIVS
                     VGAGFVRGDRPTSVQLVGIAAAIVGVLLASRAPEGHGEAVTTRAVVLSSIAAVAFGAL
                     LVFLDLGARIDPAWTTVSVRIGAVVLLVPILVFVRPSFTMTRHQFGTLVMVGILDNGA
                     NLSFAIAASTGQLLSVIAVLAALYPVATVLLARGVLHERLARSQTIGVVAAFAGIGLI
                     ALG"
     gene            1357..>2025
                     /locus_tag="VIX62_10000"
     CDS             1357..>2025
                     /locus_tag="VIX62_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860605.1"
                     /translation="MEPVDLTTLADDVQRGPLFDRQRAMGATFFEDYGRLWTASFGDP
                     EAEYRQAREGAVIWDVSALSKWHVTGPDALAAMDRLTTRPTAPDRPGQVRYVMLLNAD
                     GLLVEEATRYVLGPQEVWLVGNEDRPAMAAHIEGSIVDLDVRVENRTDEVVSIAVQGP
                     HSCEVLAPLVSGNLAALGYYHVIGRTTVAGVEAMVSRTGFSGELGYELFVFGGADAAG
                     VVWDA"
BASE COUNT          276 a          617 c          753 g          379 t
ORIGIN      
        1 aacgacccga aggtgcaacc tgcggtgcgc gagaagatgc tcgggctggg taggccggag
       61 ctcgatgcgt tccggctggc tgcggaggcc ggcgtgaagg tcgcgatggg caccgattgt
      121 ccggtggctc cccacggcac caacctcaac gagctccgcc acatggcggc gaacgggttc
      181 acccccgctc agtcgttaca tgccgccacg aagagtgcag cggagttgat gggcttgcag
      241 gacgaactcg gcaccttgga gcccggcaag cgagccgatg tggtcgtggt cgacggcgac
      301 ccgctcgact tccaggacta cgaatcgcgc atccaccagg tatggaagga cggcgtgcgc
      361 gtcagctagc ctcccgcgat ggtggggatc gtccttgcgc tcgtcggcgc cgctgggtgg
      421 ggaaccgccg acttcttggg tgggttgtcc acgaagaagc tgtcgatctg ggtggtcagc
      481 gtcgtgtcgc agatcgccgg cttggtgttc atggccgtgg tcgtggtcag ccttggtcgt
      541 gggccggtcg agatgaaggt cgtctggctc ggcatggcgg cgggcgtctg cgggctgatc
      601 ggtctgtcgg cgttgtacac cgcgctcgcg ctcggaccga tgggagtggt ggcgcccatc
      661 tcagcgctct ccgtgatcgt gtcggtgggc gccggtttcg tgcgaggtga ccgaccgacg
      721 tccgtgcaac tcgtggggat cgcggccgcg atcgtgggtg tgctcctggc atcgcgcgcg
      781 cctgagggcc acggcgaggc cgtcaccact cgcgcggtgg tgctgtcctc gatcgccgcg
      841 gttgcgttcg gggccctcct cgtcttcctg gatctcggcg cccgcatcga tcccgcgtgg
      901 accacggtgt cggttcgcat cggcgcggtg gtccttctgg tgccgatcct ggtgttcgtc
      961 cgaccgtcgt tcacgatgac ccggcaccag ttcggcacgt tggtgatggt ggggatcctc
     1021 gacaatggcg cgaacctgtc gttcgcgatc gcggcatcga caggccagct gctcagcgtg
     1081 atcgcggtgc ttgcggcgct ctaccccgtc gccaccgtgc tgctcgcgag gggcgtgttg
     1141 cacgagcgcc tggcccgttc acagaccatc ggtgtggtcg ccgccttcgc ggggatcggt
     1201 ttgatcgccc tggggtagtg gtagtgggct cggggtcccc cgatctcccc gatgtggtac
     1261 tcgccggagg tgcggcgtgg gaggctgcgc accagtggct gttggccgcg ccggggtccg
     1321 aaccgttcgg gccgttcaac aacgaaggga atcatcgtgg aaccggtcga cctcaccacg
     1381 cttgccgacg acgtgcagcg tgggcccctg ttcgatcggc agcgcgcgat gggagccacc
     1441 ttcttcgaag actacggacg tctgtggacg gcctcgttcg gagatccgga ggccgagtat
     1501 cggcaggcgc gcgagggcgc agtgatctgg gacgtctcgg cactgtcgaa gtggcacgtc
     1561 accggccccg acgcgctcgc ggcgatggac cggctcacca ccaggccgac cgcacccgac
     1621 cgaccggggc aggttcggta cgtgatgctc ctcaatgcag acgggctgtt ggtcgaggag
     1681 gcgacccggt acgtgttggg tccgcaggaa gtctggttgg tggggaacga ggatcggccc
     1741 gcgatggcgg cacacatcga ggggtcgatc gtcgacctcg atgtgcgcgt cgagaatcgc
     1801 accgacgagg tggtgagcat cgccgtgcaa gggcctcact cttgcgaggt gctggcgccg
     1861 ctcgtctcgg gaaaccttgc cgcactcggc tactaccacg tgatcgggcg cacgacggtc
     1921 gccggtgttg aggcgatggt gtcgaggacc gggttctcgg gcgaattggg gtacgagctg
     1981 ttcgtgttcg gcggcgccga cgcggcgggg gtcgtatggg atgcg
//
LOCUS       DATDBZ010000418         3592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_115509, whole genome shotgun sequence.
ACCESSION   DATDBZ010000418 DATDBZ010000000
VERSION     DATDBZ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115509"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..720
                     /locus_tag="VIX62_10005"
     CDS             88..720
                     /locus_tag="VIX62_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5860606.1"
                     /translation="MARSTRDLAGLALGVGTLVGTAALARRPISRTEILAFRAVNDLP
                     EAGWPAIWLPMQYGTFGTVPASAVLAFARNRPRLALAIGVGGTAAWLSAKACKRLIGR
                     ERPAGVIGDVQLRGVEEGDLGFPSGHAAVSAALTVVALPSVTDGWRCSLAALSGFVPL
                     ARMYVGAHLPLDVVGGSALGLAIGSTVNLLIPARPGGEGPDPEEPGIGPP"
     gene            complement(717..1127)
                     /locus_tag="VIX62_10010"
     CDS             complement(717..1127)
                     /locus_tag="VIX62_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5860607.1"
                     /translation="MSTRLNPYIAFDGNAKEAMEFYRDVFGGSLNISTFGEFGNDDPA
                     VADKTMHAMLETDNGLTLMASDGPPGAELTRGDNITISLSGEDAEDLRGYWNKLSDGG
                     SVTMPLEKQMWGDEFGMCVDRFGIPWMVDIVPAS"
     gene            complement(1238..2143)
                     /locus_tag="VIX62_10015"
     CDS             complement(1238..2143)
                     /locus_tag="VIX62_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEY5860608.1"
                     /translation="MSTPQSGILALGTTSHAFLELDLRADRKGPELVRAIASLREPRT
                     TIGGVNLVAGFRPERWREAAPEDAPADAEGFGHDLVGIEGFTMPATQHDAVLWLSGGS
                     YDVVFDTAHAALAELDALAAVAEETACWPYHHDRDLTGFVDGSENPSLIEAAQIALIA
                     EGSPGEAGTILLLQRWRHDAVAWGSLGVPQQEQAMGRTKSESVELDDKAPSSHVARTD
                     QDRFGKVFRRNMPYGTVTDHGTMFVGFSAEQRRLTAMLESMAGLTDGVRDALTIYTQP
                     LTGAYYFVPSTEAISRCSESQVAPI"
     gene            complement(2199..2675)
                     /gene="bfr"
                     /locus_tag="VIX62_10020"
     CDS             complement(2199..2675)
                     /gene="bfr"
                     /locus_tag="VIX62_10020"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEY5860609.1"
                     /translation="MQGSPKVIEALNDLLTLELTVVNQYFVHSRMCVNWGYGRIAGQQ
                     RAIAMDEMRDAEQIIDRILFLDGVPNMQRLGSVTVGETVSEQFQIALEGEHRALELLH
                     SGIGIALEEGDSASREFFAGRLLEEEAHVDYAETQLGLIQRLGEANYLAQQMADPS"
     gene            complement(2686..2937)
                     /locus_tag="VIX62_10025"
     CDS             complement(2686..2937)
                     /locus_tag="VIX62_10025"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5860610.1"
                     /translation="MPRSRTEDPPASRARHAVYVCLCLAISDRAIRAAVAGGARTLES
                     LGEVSDAGTVCGGCIEELERLLRERSNGNDGAPTTEQPT"
     gene            2990..>3592
                     /locus_tag="VIX62_10030"
     CDS             2990..>3592
                     /locus_tag="VIX62_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860611.1"
                     /translation="MALLWHRFLPAMPEWHGLAMREPFLAVGLLGMCVSLGLAMSFWK
                     LAPAASMALVGGLAGATAGLVGWVFVPENLQQLDLIDGGIQASYGDGLPAAARGFTLG
                     IVIIGLVSLRLARAPTGFPASLRRASVGVLAGGVGLAWVVRNLLDGECARLGTQSCSE
                     TPMVAGSIILDAVAVGTLLLVSASATPMRERPPRRPSIPPP"
BASE COUNT          515 a         1238 c         1175 g          664 t
ORIGIN      
        1 ccggatgagt cgcaggagtc aaccacggga gcaacgagcc tggcaatcct ccgaacggca
       61 acgtggcggg gcacggtagg ctgcagcgtg gcccggtcga ctcgagatct tgccggtctt
      121 gccctcggtg tcggcaccct cgtcggaacc gcggcgctcg cgcgccgacc gatctcccgg
      181 accgagattc tggcgttccg ggccgtgaac gacctcccgg aagccggctg gccggcgatc
      241 tggctcccga tgcaatacgg gaccttcggc acggtcccag catcggccgt tctcgccttc
      301 gcccgcaacc gaccgcgcct cgcgctcgcg atcggggtgg gcggcaccgc cgcctggctg
      361 tcggcgaagg catgcaagcg gctcatcggc cgggaacgtc cggcgggcgt catcggcgac
      421 gttcagctca gaggtgttga agaaggcgac ctcggcttcc catccggaca cgcggcggtc
      481 tctgccgccc tgacggtggt tgctctgccg tccgtgacgg acggttggcg gtgttcgctc
      541 gcagcgctgt cggggttcgt gcccctcgcg cggatgtacg tcggagctca ccttccactc
      601 gacgtggtcg gtggttccgc actcggtctc gcgatcggca gcaccgtcaa cctgctgatc
      661 ccggcgcggc cgggaggcga agggcccgac ccggaggagc ctgggatcgg accgccctag
      721 gacgccggca cgatgtcgac catccacggg ataccgaacc ggtcgacgca catgccgaac
      781 tcgtctcccc acatctgctt ctcgaggggc atcgtgaccg agccaccgtc ggagagcttg
      841 ttccagtagc cccggagatc ctcggcgtct tccccgctca gcgagatcgt gatgttgtcg
      901 ccgcgagtga gctcggcgcc cgggggccca tccgacgcca tgagggtgag cccgttgtcc
      961 gtctcgagca tcgcgtgcat cgtcttatcg gccaccgcgg gatcatcgtt cccgaactca
     1021 ccgaacgtgc tgatgttcaa cgacccgccg aacacgtcac gatagaactc catcgcctcc
     1081 ttggcgttgc cgtcgaatgc gatgtacggg ttgagacggg tcgacatgat ccctcctcgc
     1141 tcgtggtggt cggctcagat cgggaacctg atcctgaagc taggcccacg tcttcggctt
     1201 cgtcaaccga cggcgggctc agacggatcg gctgatccta gatcggtgcg acctggctct
     1261 cggagcacct cgatatcgct tcggttgatg ggacgaagta gtaggcaccg gtcagtggct
     1321 gcgtgtagat cgtgagcgcg tcacgaacgc cgtccgtgag gccggccatg ctttccagca
     1381 tggccgtcag cctgcgttgt tccgcgctga agccaacgaa catcgtgccg tgatcggtca
     1441 ccgttccgta cggcatgttg cgccggaaaa ccttgccgaa ccggtcttgg tccgttcgtg
     1501 ccacgtggga ggacggcgcc ttgtcgtcga gctccacgct ctccgacttc gtgcgtccca
     1561 tcgcctgctc ctgctggggg acgccgaggg atccccatgc gaccgcgtcg tggcgccacc
     1621 gctgcaggag caggatcgtt cctgcttccc cggggctccc ttcggcgatg agcgcgatct
     1681 gggccgcctc gatcagactc gggttctcgc tcccgtccac gaacccggtc agatctcggt
     1741 cgtggtggta gggccagcag gcggtttcct ccgctaccgc agcgagcgcg tcgagttcgg
     1801 cgagcgcggc gtgagccgtg tcgaagacca cgtcgtacga acccccggag agccagagca
     1861 cggcgtcgtg ttgggtcgcc ggcatcgtga agccctcgat accgacgagg tcgtgcccga
     1921 agccctcggc gtccgccgga gcgtcctccg gcgccgcctc ccgccaacgc tccggccgga
     1981 agccggcgac gaggttgacg cctccgatgg tggtcctcgg ctcccggagc gacgcgatcg
     2041 cgcgcacgag ctcggggccc ttccgatcgg cgcgcaggtc gagctccagg aaggcgtgtg
     2101 acgtggttcc gagggcaagg atcccgcttt gcggagtact cacgttcccg atgatgcagg
     2161 aggccgacaa gagcgtccag ccggtcgcgc ctcgagagct atgaggggtc tgccatctgc
     2221 tgggcgaggt agttcgcctc acccaatcgc tggatcaagc ccagctgggt ctcggcgtag
     2281 tccacgtggg cctcctcttc gagcaacctg cccgcgaaga actcgcggct cgccgagtca
     2341 ccttcttcca gggcgatccc gatccccgaa tgcagcaact ccagcgcccg atgctcccct
     2401 tccagggcga tctggaactg ttcgctgacg gtttcgccaa ccgtgaccga tccgagccgt
     2461 tgcatgttgg gcacgccgtc caggaacaag atgcgatcga tgatctgctc cgcatcacgc
     2521 atctcgtcca tcgcgatcgc gcgctgctgc ccggcgatcc gtccgtagcc ccagttcacg
     2581 cacatccgtg aatgcacgaa gtactgattg accaccgtca gttcgagcgt gagcaagtcg
     2641 ttcagtgctt cgatgacctt cgggcttccc tgcacgtgcc cctcctcaag tcggttgctc
     2701 ggtcgttggt gctccgtcgt tgccgttcga tcgctcccga agcagccgct ccagctcctc
     2761 gatgcatccg ccgcacacgg tgccggcgtc gctgacctcc cccaacgact cgagggttcg
     2821 cgctccgccg gcgaccgccg cgcggatcgc tcgatcggag atcgccagac acaggcagac
     2881 gtacacagca tgcctcgctc tcgatgcggg cggatcctcg gttcgggatc gaggcacagc
     2941 ctagcggtca tcccgagaac gcaccgatgt gaaggtttgc acggtacgcg tggcattgct
     3001 gtggcaccgc ttccttcctg cgatgccgga gtggcacggt ctcgccatgc gcgaaccgtt
     3061 cctggcggtc ggactcttgg ggatgtgcgt gtcccttggt ctcgcgatgt ccttctggaa
     3121 gctggcaccc gccgcgtcca tggccctcgt cggcggcctc gccggcgcga ccgccgggct
     3181 ggtcggctgg gtgttcgtcc cggagaacct tcagcagctg gatctgatcg acggaggcat
     3241 ccaggcgagc tacggggacg gtctccccgc tgccgcccgg gggttcaccc tcgggatcgt
     3301 catcatcggg ctggtgtcgc tgcgtcttgc tcgcgcaccg acgggcttcc ccgcgtccct
     3361 acgccgggcc tccgtcggtg tcctggccgg gggtgtcggg ttggcgtggg tcgttcgcaa
     3421 cctgctggac ggtgagtgcg cgcgactcgg gacgcagtcg tgctccgaga ccccgatggt
     3481 ggccggcagc atcatcctcg atgccgtggc ggtggggacc ctgctgctgg tgtcggcgtc
     3541 cgccacgccg atgcgtgaac gtccgcctcg gcgcccttcg atccctccgc ca
//
LOCUS       DATDBZ010000419         2473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_764635, whole genome shotgun sequence.
ACCESSION   DATDBZ010000419 DATDBZ010000000
VERSION     DATDBZ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764635"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VIX62_10035"
     CDS             <1..338
                     /locus_tag="VIX62_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HEY5860612.1"
                     /translation="RGLDRAGLGSNLTRAEMEALGFFVCVADLEDELIRALGPTSVLQ
                     LVEAQGELRSFRTLQKQPAQQGRSVEAQLRRFMGSRGGRKIHYAPLLIDALDLARVPR
                     PLAGVLAHV"
     gene            393..1322
                     /locus_tag="VIX62_10040"
     CDS             393..1322
                     /locus_tag="VIX62_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEY5860613.1"
                     /translation="MSEDTDGDGGLLANFWPALPVAEWQATRDTLQLWTQIIGKIRMV
                     RTPMTSHWWNVPLYVTARGLSTSMIPDAGRGFQIDFDFTTHRLDITTSDGAQRTMPLE
                     ARSVAEFYMAVMAMLDELDLATPIWTMPVEIPDAIPFDQDVVHASYDRDQVERFWRAL
                     IEMVRVFEQFRAEFVGKVSPVHLFWGALDLAVTRFSGRPAPPHPGGAPNCGPHVMLEA
                     YSHEVSSAGYWPGPDGEGVFYSYAYPEPDGYRHQPIAPSAAHFDDTLGEFVLPYEIVR
                     TAPDPDAVLLDFLHTTYAAAADTARWNRKELER"
     gene            1353..1664
                     /locus_tag="VIX62_10045"
     CDS             1353..1664
                     /locus_tag="VIX62_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY5860614.1"
                     /translation="MAEVSCAHLATISATIDDVVASSPGCEDCLRIGGRWVHLRMCMH
                     CGHIGCCDNSPNRHATAHWHDHPDHPIIRSYEPGEDWWWCYPDDLFFEVDDAPQSPSH
                     P"
     gene            complement(1698..>2473)
                     /locus_tag="VIX62_10050"
     CDS             complement(1698..>2473)
                     /locus_tag="VIX62_10050"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5860615.1"
                     /translation="RVPVLSPSLSARWVGLVTPLPSSIARPLIDSLRHEVVMTDHDID
                     SLVPHQPLTFRESLELALRHTRAEAIDTRWTDAGFASADAIPGDPDWAGGSLFEDHQT
                     VDSTATVEDLYQAFARIGGENGYYVEDWAWSLRGLADKLIGGPGLRRGRRHPVDLRPG
                     ESLDFWRVVEVEPGHQLVLQAEMKVPGKAWLTWRIEPGDAPDTLQLHQVAFFAPKGLF
                     GRLYWYTMLPFHWLIFRRMANAIADHAARGGVEPAPVHG"
BASE COUNT          420 a          881 c          791 g          381 t
ORIGIN      
        1 gacgcggcct cgaccgcgcg ggcctcggct ccaacctcac ccgcgccgag atggaggctc
       61 tgggcttctt cgtctgtgtg gccgacttgg aggacgagtt gatccgcgcc ctcggtccca
      121 cctcggtgct gcagctcgtc gaagcgcagg gtgagctcag atcgttccgc accctccaga
      181 agcagcccgc ccagcagggg cgtagcgtcg aggcgcagct gcggcgcttc atgggaagcc
      241 gcggcggccg caagatccac tacgcaccgt tgctcatcga cgcgctcgat ctcgcccgcg
      301 taccgcggcc actggcgggc gtgcttgcgc acgtgtgacg caaccgttgc tcgacgaaca
      361 tgcggaccgg gcaccgcgcg tacgatcgac acatgagcga ggacaccgac ggcgacggcg
      421 gcttgctcgc caacttctgg ccagcgcttc cggtcgccga gtggcaagcg acgcgcgaca
      481 cgttgcagct gtggacccag atcatcggca agatccgaat ggtccgcacg ccgatgacgt
      541 cacactggtg gaacgtgccg ctgtacgtga cggcgcgggg cttgtcgacg tcgatgatcc
      601 ccgacgcggg gcgcgggttc cagatcgact tcgacttcac gacccaccgt ctcgacatca
      661 ccaccagcga cggcgcgcag cgcaccatgc cactcgaagc tcgctcggtc gccgagttct
      721 acatggccgt gatggccatg ctcgacgaac ttgacctggc aacacccatc tggacgatgc
      781 ccgtcgagat ccccgacgcg atcccgttcg accaggacgt cgtccacgcg tcgtacgacc
      841 gcgaccaggt tgagcggttc tggcgggcgc tgatcgagat ggttcgcgtg ttcgagcagt
      901 tccgcgccga gttcgtcggc aaggtcagcc cggtccacct gttctggggc gcgctcgacc
      961 tcgccgtcac ccgcttctcc ggccggcccg caccgccaca tcccggaggc gcaccgaact
     1021 gtggcccgca cgtgatgctc gaggcctact cacacgaggt cagcagtgcg ggctactggc
     1081 cgggacccga cggtgagggt gtcttctact cgtacgccta tcccgagccc gacggatacc
     1141 gccatcaacc gatcgcacca tccgccgccc acttcgacga cacactcggc gagttcgtgt
     1201 tgccgtacga gatcgtccgc accgcgcccg atcccgacgc tgtgttgctc gacttcctcc
     1261 acaccaccta cgcggcggcc gccgatacgg ctcgctggaa tcgcaaggag ctcgaacgat
     1321 gaggcccgaa cgatgaaccc cgaaccatga aaatggcaga agtgtcgtgc gcccacctcg
     1381 ccacgatctc ggcgacgatc gacgacgtcg tggcatccag cccgggctgc gaggactgcc
     1441 tgcgcattgg cggacgatgg gtgcacctgc gcatgtgcat gcactgtggg cacatcggct
     1501 gctgcgacaa ctctccgaac cgtcacgcca ccgcccactg gcacgatcac cccgatcacc
     1561 cgatcatccg ctcgtacgaa cccggcgagg actggtggtg gtgctacccc gacgacttgt
     1621 tcttcgaggt cgacgacgcc ccccagtcac cgagccatcc ctgacgccca tcacccacgg
     1681 cgattccccg ggaacggtca gccgtgaacg ggcgccggct ccaccccgcc gcgcgccgcg
     1741 tggtcggcga tggcgttggc catgcggcgg aagatgagcc agtggaacgg cagcatggtg
     1801 taccagtaga gacgcccgaa caagcccttg ggggcgaaga acgccacctg gtgcagttgc
     1861 aacgtgtccg gcgcgtcgcc cggttcgatg cgccacgtca gccacgcctt tccgggcacc
     1921 ttcatctcgg cctggagtac cagctggtgg cccggctcga cctcgacgac gcgccagaaa
     1981 tcgagcgatt caccaggccg caggtcgacg gggtggcgcc ggccgcgccg taggccgggc
     2041 ccgccgatca gcttgtcggc cagcccgcgc agcgaccacg cccagtcctc gacgtaataa
     2101 ccgttctcac caccgatgcg ggcgaacgcc tggtagaggt cctccaccgt ggcggtgctg
     2161 tcgaccgtct ggtggtcctc gaagagcgag ccgccagccc agtcaggatc gccaggaatc
     2221 gcatctgccg acgcgaaccc ggcgtcggtc caacgagtat cgatcgcctc ggcgcgggtg
     2281 tgtcggagcg cgagctcgag cgactcgcgg aacgtcagcg gttggtgcgg caccagggag
     2341 tcgatgtcgt ggtcggtcat caccacctcg tggcgcagcg agtcgatcag cggcctcgcg
     2401 atcgacgagg gcagcggcgt gacgaggccg acccaccgtg ccgacaggct gggcgacagg
     2461 accggaaccc gca
//
LOCUS       DATDBZ010000420         3017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_542516, whole genome shotgun sequence.
ACCESSION   DATDBZ010000420 DATDBZ010000000
VERSION     DATDBZ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542516"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VIX62_10055"
     CDS             complement(<1..314)
                     /locus_tag="VIX62_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860616.1"
                     /translation="MVLGEGEPVVDVVRGVVSLPDLPAPALTDHLPEGPSWRYEPDWD
                     GLRVVVHRRAGHTVLVSDRGKPMDRYFPEVVRHASRLPFDCTVRGSIVVVRRSGFSFD
                     RLR"
     gene            464..1387
                     /locus_tag="VIX62_10060"
     CDS             464..1387
                     /locus_tag="VIX62_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5860617.1"
                     /translation="MGGTAGIGLGCMPMSFGYVDAEADDDPVDVIHRAIDLGAPMLDT
                     ADVYGPFSNEELVGAAIHDRRDRVEVATKVGLLVGPNGGYPLDRDASPHHVRSAIDAS
                     LRRLRTDVVDLYYLHRIDPNVPVEETWGAMAELVGAGKVRALGLSEVTVAGAERAHAI
                     HPVAAIQSEMSLWTRDPLADIVLWCAEHGARFVPFSPLGRGFLTGAISAASFDESDFR
                     SNNPRFTTEAVDANQAIVARVREVAERVGATSAQVALAWTLAQGEHVLPIPGTKRRRY
                     LEENLAASDLVLSPEDLADLDALPVAEGARY"
     gene            complement(1391..1822)
                     /locus_tag="VIX62_10065"
     CDS             complement(1391..1822)
                     /locus_tag="VIX62_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5860618.1"
                     /translation="MSVPAKINIVTLGVADIERAAAFYSALGWERCASGGDEITWFRT
                     HGAYLGLFGYASLAADADLPPEPRAPFGGITLAINVEAEHAVTDALDAAVTAGGTLLK
                     AAERMDWGGFSGYFADPDGHPWEVAFNPFFPIGADGSITID"
     gene            1876..2706
                     /locus_tag="VIX62_10070"
     CDS             1876..2706
                     /locus_tag="VIX62_10070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860619.1"
                     /translation="MVEANPLLQRIDAYLDGVPRGVTITEEFGPFTLFINTGNGWRYY
                     ARPTPGQTLFDVDSVEAVLARQRELGQPQEFEWVADLAPGVRDATEAAGLEVSEMPLM
                     HLPLDGFRAVGPEDGEVDVRIVGPEDDLATAIAVATVGFGAPGTDVGEAGIEALPTAA
                     AAIPAGTLEFTADRIRDGFTSMAVATVGGIPVASGSHQPFGGATEITGVACLPAYRRR
                     GLGAAVTSALVEDAIDRGVGTVFLSAGGEDIARVYQRVGFRTVGHVGAAAFADAATLR
                     "
     gene            complement(2715..>3017)
                     /locus_tag="VIX62_10075"
     CDS             complement(2715..>3017)
                     /locus_tag="VIX62_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY5860620.1"
                     /translation="DDPDPWPRPMSLEQNLSDLEQHAKDFQDRVGFTYTVLDPVDRDV
                     IGCVYLYPSPLEGVDVRARSWVRRSRAELDEPLRRAVRAWLGGPEWPFERIDYAAG"
BASE COUNT          467 a         1059 c         1076 g          415 t
ORIGIN      
        1 cgcagacgat cgaaggagaa cccggaccgc cgaaccacca cgatcgaacc gcgcaccgtg
       61 cagtcgaagg gaaggcgtga cgcgtgccgc acgacctcgg ggaagtagcg gtccatcggc
      121 ttcccccggt cggacaccaa cacggtgtgc cccgcacgcc ggtgcaccac gacgcgcagc
      181 ccgtcccagt ccggttcata gcgccatgag gggccctcgg ggagatgatc ggtgagggct
      241 ggagcgggca ggtcgggcag cgacacgacg ccgcgaacga cgtcaacgac cggctccccc
      301 tcacccaaga ccatcgtgtc gaccttccca tcggaggaca ccggaacaca tcgggcgctc
      361 agcccaaacc ccaaggccga tgggccgagt cgcattgggc cggacggggt ccccccgacc
      421 ggactacagc ggcgctgcgt ggtggcggcg atagggtgca tacatgggag gaaccgccgg
      481 gatcgggctg gggtgcatgc cgatgagctt cggctacgtc gacgccgaag ccgacgatga
      541 cccggtcgac gtgatccacc gcgccatcga cctgggtgct ccgatgctcg acaccgccga
      601 tgtgtacggg ccgttctcca acgaggagct cgtcggcgcg gccatccacg accgccgcga
      661 ccgcgtggag gtggccacca aggtgggcct gctcgtggga ccgaacggtg gctacccgct
      721 cgaccgcgac gcgagccctc accacgtgcg gtcggcgatc gacgcttccc tccgacggct
      781 gcgcaccgat gtggtggacc tctactacct ccaccgcatc gatccgaacg tccccgtcga
      841 ggagacgtgg ggcgcgatgg ccgaactcgt cggcgccggc aaggtgcgag ccctcgggct
      901 ctcggaggtc accgtcgcgg gggccgaacg ggcgcacgcg atccatcctg tcgcggcgat
      961 ccaatcggag atgagcctgt ggacgcgtga tccgctcgcg gacatcgtgc tctggtgcgc
     1021 cgagcacggc gcccggttcg tgccgttctc cccgctgggg cgcgggttcc tcacgggagc
     1081 gatctcggcg gcgagcttcg acgagtccga cttccggtcg aacaatccgc gcttcaccac
     1141 cgaggcggtc gacgcgaacc aggccatcgt ggcgcgggtt cgcgaggtgg ccgaacgcgt
     1201 gggcgccacg tccgcgcagg tcgcgctcgc gtggacgttg gcccagggcg agcacgtgct
     1261 gccgatcccc ggcacgaagc gtcggcgcta cctggaggag aacctggccg ccagcgacct
     1321 cgtgctctca cccgaggacc tcgccgatct ggacgcgctg cccgtcgccg aaggcgcccg
     1381 gtactgacgc tcagtcgatc gtgatcgagc cgtcggcccc gatcgggaag aacgggttga
     1441 aggcgacctc ccacggatgc ccgtcgggat cggcgaagta gccggagaac ccgccccagt
     1501 ccatgcgttc ggcggccttc agcagcgtgc cgcccgcggt gaccgcggca tcgagcgcgt
     1561 cggtgacggc gtgctcggcc tcgacgttga tcgccagcgt gatgccgccg aacggcgcgc
     1621 gaggctccgg aggcaggtcg gcgtcggccg ccagcgaggc gtagccgaac aaccccagat
     1681 aggcgccgtg ggtgcggaac caggtgatct cgtcaccacc ggaagcgcat cgttcccacc
     1741 ccagtgcgga atagaacgcg gccgcccgtt cgatgtcggc gacccccagg gtgacgatgt
     1801 tgatcttggc cggaacgctc atgccgccga tgctagtcgg ccgccacgac atcgccgacc
     1861 tcgtagcctg cacgcatggt cgaagccaac ccgctcctgc aacgcatcga cgcctacctc
     1921 gacggtgtgc cgcgcggcgt caccatcacc gaggagttcg gtcccttcac cctgttcatc
     1981 aacaccggca acgggtggcg ctactacgcg cgaccgacgc cgggccagac cctgttcgac
     2041 gtggacagcg tggaggccgt gctcgcccgc caacgcgaac tcggccagcc gcaggagttc
     2101 gaatgggtag ccgacctcgc ccccggggtc cgcgacgcca ccgaggccgc ggggctcgag
     2161 gtgagcgaga tgccgctgat gcatctgccg ctcgacgggt tccgagcggt cggcccggag
     2221 gacggggagg tcgacgtccg gatcgtcggt cccgaggacg acctcgcgac cgccatcgcc
     2281 gtcgccacgg tgggcttcgg cgcgccgggc accgatgtgg gtgaggccgg gatcgaggcg
     2341 ttgcccacgg ccgcggcggc gatccccgcg ggcacgctcg agttcacggc cgaccggatc
     2401 cgcgacgggt tcacgtcgat ggcggtggcc accgtcggcg ggatcccggt cgcgagtggg
     2461 agccaccagc ccttcggggg cgccacggag atcaccggcg tggcgtgtct gccggcttac
     2521 cggcggcgcg gcctgggggc cgcggtgacc tcggcgttgg tggaggacgc catcgatcgc
     2581 ggggtgggga cggtgttcct gtcggcgggc ggcgaggaca tcgcgcgggt gtaccaacgc
     2641 gtgggcttcc gcacggtcgg tcacgtgggt gcggccgcgt tcgccgatgc cgcaacgctc
     2701 cgctagcggg cgggtcagcc cgcggcgtag tcgatccgct cgaacggcca ctcggggccg
     2761 ccgagccatg cccggaccgc acgccgaagc ggctcgtcca gctcggcgcg cgagcgccgc
     2821 acccaggatc gggcacgcac gtccacaccc tcgaggggcg acgggtagag gtagacgcac
     2881 ccgatcacgt cgcgatccac gggatcgagc accgtgtacg tgaatcccac acggtcctgg
     2941 aaatccttcg cgtgttgctc gaggtcggac aggttctgct ccaggctcat cggccgcggc
     3001 caggggtcgg ggtcgtc
//
LOCUS       DATDBZ010000421         2333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2597407, whole genome shotgun sequence.
ACCESSION   DATDBZ010000421 DATDBZ010000000
VERSION     DATDBZ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2597407"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VIX62_10080"
     CDS             complement(<1..323)
                     /locus_tag="VIX62_10080"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEY5860621.1"
                     /translation="MIETTTGPTPGSERDFSMGADPIDVPVIPPPIEGVDRTEWNDLV
                     ERGRESGELHAEQVAHVLRHVELTGDAIMAVSEALASAGIEVDDSVPDPDDPTPTRGT
                     TRRVD"
     gene            complement(320..2065)
                     /gene="dnaG"
                     /locus_tag="VIX62_10085"
     CDS             complement(320..2065)
                     /gene="dnaG"
                     /locus_tag="VIX62_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY5860622.1"
                     /translation="MAIADDDIERIRSTVSIVDVIGQHVQLKRTGRNWVGLCPFHAER
                     TPSFNVREETGRYRCFGCDKSGDVFTFVQEIEHTDFVGAIEQLASRAGIQLTYTSTGQ
                     SKERARRKRLVEAMTAAVDWYHARLLTSPDARDARDYLRSRGLSGDIARQFKLGWAPD
                     DWDALARESGIDPGLLRDNGLAFTNRRNRMQDAFRARVLFPIFGENGEALALGGRILP
                     GSDDPAKYKNSAETPIYTKSKTLYGLNWAKGDIVNSNQVIVCEGYTDVIGFHRAGLPR
                     AVATCGTAFTEEHVRLLKRYGSEIVLAFDADNAGQTAAERFYDWEEKYQVEVKVASFP
                     GGKDPGEMAQSDPTALAEAVGSAKPFLGFRLGRVLDGKVPQSPEAVARLGEAAMAVVN
                     EHPNTDIRKLYAGEVALRVGLPVNDMVAVAERRTRRPHMAVAQPKPRSQRRQNGEFVA
                     ISLLVQEWDSIAPWLVEALFVDEVYRRAFIAVAEAGGVLDAALEAADPEARDVIERAA
                     VIDVNANGEAEALNLIAAAVRRILTSGRTSEDPEVIRRDAEARVKMEQLGERDRNLEA
                     AGWLLGWLESRRDSP"
     gene            complement(2122..>2333)
                     /locus_tag="VIX62_10090"
     CDS             complement(2122..>2333)
                     /locus_tag="VIX62_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860623.1"
                     /translation="VKMLIHSWYHIPTWLSLTVIVIVLAASIGFSLKVERTAAESELA
                     GEAFEPREPSEPHEPSEMDQPPHHD"
BASE COUNT          353 a          850 c          732 g          398 t
ORIGIN      
        1 atgtcgactc tgcgggtcgt cccacgcgtc ggggtcgggt cgtcgggatc gggcacggag
       61 tcgtcgacct cgatccccgc cgaggccagt gcctcgctca ccgccatgat cgcatccccg
      121 gtcagctcga cgtgacgcaa cacgtgggcg acctgctcgg cgtgcaactc gcccgactcg
      181 cggccgcgtt ccaccaggtc gttccattcc gtgcgatcca ccccttcgat cgggggtggg
      241 atcaccggca cgtcgatcgg gtcggccccc attgagaagt cacgctcact gccgggtgtg
      301 ggcccggtcg tggtctcgat cacggcgaat cccgccgtga ctccagccaa cctagcaacc
      361 atccggccgc ctcaaggttt cggtcccgct caccgagttg ctccatcttg actcgcgcct
      421 cggcgtcgcg acggatcacc tcgggatctt cgctcgttcg accagacgtc aggatgcgcc
      481 gcaccgccgc ggcgatcaga ttgagcgcct cggcctcgcc gttcgcgttc acgtcgatga
      541 ccgccgcacg ctcgatcaca tcgcgcgctt cggggtcggc cgcctcgagc gccgcgtcga
      601 gcacgccgcc cgcctcggcg acggcgatga acgctcgccg gtacacctcg tcgacgaaca
      661 gcgcctcgac aagccacggc gcgatcgagt cccactcctg gaccagtagc gagatcgcga
      721 cgaactcgcc gttctggcgc cgctgggacc gcggcttggg ctgagcgacc gccatgtgcg
      781 ggcgccgggt ccggcgctcc gcgaccgcca ccatgtcgtt cacgggcagg cccactcgga
      841 gcgccacctc gccggcgtac agctttcgga tatcggtgtt gggatgctcg ttcacgaccg
      901 ccatcgccgc ctcacccagc cgcgcgaccg cctccggcga ctgtggaacc ttcccgtcga
      961 gcacccgacc cagccggaac ccgaggaacg gtttggcgct tccgaccgcc tccgcgagcg
     1021 cggtcggatc ggactgggcc atctcgcccg ggtccttgcc gccggggaac gaggccacct
     1081 tcacctccac ttggtacttc tcctcccagt cgtagaaccg ttcggccgcg gtctggccag
     1141 cattgtccgc atcgaacgcg agcacgatct cgctcccgta ccgcttgagc agccggacgt
     1201 gttcctcggt gaaggccgta ccgcacgtcg ccaccgcccg cggcaagccc gcccggtgga
     1261 acccgatcac gtcggtgtac ccctcgcaga cgatcacctg gttggagttg acgatgtcgc
     1321 ctttggccca gttgagcccg tacagcgtct tcgacttggt gtagatcggt gtctcggccg
     1381 agttcttgta cttggccgga tcgtcggatc ccggcaggat ccgcccgccc agcgcgagcg
     1441 cctccccgtt ctcaccgaag atcgggaaga gcacccgggc ccggaacgcg tcctgcatcc
     1501 ggttgcggcg attcgtgaac gccagcccgt tgtcgcgcag caatcccgga tcgatccccg
     1561 actcgcgcgc gagcgcgtcc cagtcgtcgg gagcccaccc cagcttgaac tggcgtgcga
     1621 tgtcaccgga gagaccgcga ctgcgcaggt aatcacgcgc atcgcgcgca tcgggcgagg
     1681 tgagcaaccg cgcgtggtac cagtcgactg cggcggtcat cgcctcgacc aggcgcttgc
     1741 ggcgggctcg ctccttcgat tgacccgtcg atgtgtacgt cagttggatg ccggcgcggc
     1801 tcgccagctg ctcgatggcg ccgacgaagt cggtgtgttc gatctcctgc acgaacgtga
     1861 acacgtcgcc cgacttgtcg cagccgaagc agcggtaccg cccggtctcc tcacgcacgt
     1921 tgaacgacgg cgtccgctcg gcatggaacg ggcacagccc gacccagttc cgaccggtcc
     1981 gcttcagctg cacgtgctgg ccgatcacat cgacgatcga caccgtcgag cggatccgct
     2041 cgatgtcgtc gtcagcgatc gccatgcgcc gaatctaacc agggcggacc gagggccgac
     2101 ccagcgttgc gcacgggcgg atcagtcgtg gtggggtggt tggtccatct cggacggctc
     2161 gtgcggctcg gacggctcgc ggggctcgaa cgcttcacct gcgagttccg attccgcagc
     2221 cgtgcgctcc accttgagcg agaacccgat cgatgcggcc agcacgatca cgatcaccgt
     2281 cagcgacagc caggtcggaa tgtgatacca gctgtggatc agcatcttca ccc
//
LOCUS       DATDBZ010000422         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1146578, whole genome shotgun sequence.
ACCESSION   DATDBZ010000422 DATDBZ010000000
VERSION     DATDBZ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146578"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VIX62_10095"
     CDS             <1..1061
                     /locus_tag="VIX62_10095"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5860624.1"
                     /translation="YQGAVHGVLMMNGTVTMEVACKALDIGWGDEVIVPALTFAATAY
                     APMAAGAIPVFVDVTPETWTIDPDLVEAAITERTRAIMPVHLGHQMADMDRIMAIANT
                     HGLAVIEDCAHAPGQRWRDQGAGCIGDFGSFSHQSSKILTSGEGGSLLTNDAALAQRA
                     HSIIDCGRAKDPDEKAYTFGANYRLSDLNAALLVVAMQRFPAQQAERAVTGKAFERAV
                     SAVPGVRVMPHDERVTRWSFYNYILAIDPEMFAGATNEVVCAAMEAEGIPAEVQYPSM
                     NRYELFQPSLSRLPVAVEFADRLDMQAMSFPVAEAAGQRESVYFMENVFRDGDRGIDD
                     AVEALAKIQRHADELTKR"
     gene            complement(1069..2367)
                     /locus_tag="VIX62_10100"
     CDS             complement(1069..2367)
                     /locus_tag="VIX62_10100"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEY5860625.1"
                     /translation="MGVKVAVVGGGSTYTPELVEGFVTRGDRLPVDELVLLDIDRERL
                     DVVGALAERMMRRAGWSGRLTLTDDRVEAIDGADFVIVQLRVGGNQARYRDETIPPMF
                     GCIGQETTGPGGFAKALRTVPVVLELAELTAERGAPGAWFVDFTNPTGLVTQALLDQG
                     HRAIGLCNVAIGFQREFASMFDVDPSSVQLDHVGLNHLTWERKVLIDGEDRLPALLEA
                     LEDPDSPVQAYRRELEAETHLDLVQALRAIPSYYLHYYYDVDRVLAAQQAGETPRAEE
                     VMKIEAGLLELYRDPTLDTKPKLLEERGGAFYSDAAAALVASLHAGTGDVQVVNLRND
                     GAIANLPDDAVVEISATIDREGAHPIVTEALAPAMHGLVAHAKSYELLAIDAAISGSR
                     VTALQALMANPLVGDHERAVPLLEALLSANEAYLPRFFSA"
BASE COUNT          402 a          871 c          828 g          374 t
ORIGIN      
        1 cctaccaagg cgcggtccac ggcgtgttga tgatgaacgg cacggtcacg atggaggtgg
       61 cgtgcaaggc gctggacatc ggatggggcg acgaggtcat cgtgccggcg ctcacgttcg
      121 ccgccacggc ctacgcgccg atggcggcag gcgcgatccc cgtgttcgtc gacgtcaccc
      181 ccgagacctg gacgatcgac cccgacctcg tcgaagcggc gatcaccgag cgcacgcgcg
      241 cgatcatgcc ggtgcacctg ggccaccaga tggcggacat ggatcggatc atggcgatcg
      301 ccaacacgca cgggctcgcg gtcatcgaag actgcgccca cgcaccggga cagcgatggc
      361 gcgaccaggg cgcaggatgc atcggcgact tcggctcctt cagccatcag tcgtccaaga
      421 tcctcacgtc gggcgaaggc gggagcctgc tcacgaacga cgccgctctc gcccagcggg
      481 cgcattcgat catcgactgc ggacgcgcga aagatcctga cgagaaggcg tacacgttcg
      541 gcgcgaacta ccgactgtcg gacctgaacg ccgcgctcct cgtcgtcgcg atgcagcgct
      601 tccccgccca gcaggccgaa cgagcggtga ccgggaaggc cttcgagcga gcggtctcgg
      661 ccgtccccgg ggtgcgcgtg atgccgcacg acgaacgggt cacccgctgg agcttctaca
      721 actacatcct cgcgatcgac ccggagatgt tcgcgggggc caccaacgag gtggtgtgcg
      781 cggcgatgga agccgagggc atcccggccg aggtgcagta cccctcgatg aaccgatacg
      841 agttgttcca gccgtcgctg tcccggcttc cggtggcggt cgagttcgcc gaccggctgg
      901 acatgcaggc catgtcgttc ccggtggccg aggccgccgg ccagcgcgag tcggtctact
      961 tcatggagaa cgtcttccgg gacggtgacc gtggcatcga tgacgcggtg gaagcgctgg
     1021 cgaagatcca acggcacgcc gacgaactga cgaagcgcta gcgcccgatc aggcgctgaa
     1081 gaaccggggc aggtacgcct cgttggcgct cagcaacgcc tcgagcaacg gcacggcgcg
     1141 ctcgtggtcg ccgaccagcg ggttggccat cagcgcctgc aacgccgtga cccggctgcc
     1201 ggagatcgcc gcgtcgatcg cgagcagttc gtaggacttc gcgtgcgcca ccaggccgtg
     1261 catggccggc gcgagcgcct cggtgacgat cggatgggcc ccctcgcgat cgatcgtggc
     1321 cgagatctcc accacggcat cgtcgggcag gttcgcgatc gcgccgtcgt tacgcaggtt
     1381 cacgacctgc acgtcacccg ttcccgcatg aagcgaggcg accagcgccg cggcggcgtc
     1441 gctgtagaac gcgccgccgc gttcctccag gagcttcggt ttcgtgtcga gcgtcgggtc
     1501 gcggtacagc tccagcaggc ccgcctcgat cttcatgacc tcctcggccc gaggggtctc
     1561 ccccgcttgc tgggccgcga gcacgcgatc gacgtcgtag tagtagtgga ggtagtagct
     1621 ggggatcgca cggagcgcct gcaccagatc cagatgcgtc tcggcttcca gttcgcgtcg
     1681 gtacgcctgt acgggcgagt cggggtcctc gagcgcctcc agcaacgccg gcaggcgatc
     1741 ctccccgtcg atcagcacct tgcgctccca cgtcaggtgg ttgagcccga cgtggtccag
     1801 ctgcacgctc gaggggtcga cgtcgaacat cgacgcgaac tcccgctgga acccgatcgc
     1861 cacgttgcat agcccgatcg cgcggtgccc ctgatccagc agcgcctggg tgaccagccc
     1921 tgtcgggttc gtgaagtcca cgaaccatgc gccgggcgcg ccgcgctcgg ccgtgagctc
     1981 ggcgagctcc agcaccaccg gcaccgtccg cagcgccttg gcgaacccgc cgggaccggt
     2041 cgtctcctgg ccgatgcacc cgaacatggg cgggatcgtc tcgtcccggt agcgagcctg
     2101 gttcccgccc acccggagct gcacgatcac gaagtcggcg ccgtcgatcg cctcgacgcg
     2161 gtcgtcggtg agggtgaggc gacccgacca gccggccctg cgcatcatgc gctcggccag
     2221 ggccccgacg acgtcgagcc gctctcgatc gatgtcgagc agcaccagct cgtccaccgg
     2281 gaggcggtcg ccgcgggtca cgaacccctc gacgagctcc ggcgtgtacg tgctgcctcc
     2341 accgacgacc gcgaccttca cacccatgcc gtcggcatcg tacactcgcg ccgatgaaat
     2401 cgttaggaaa ctttcctgac ccattcatcg ccgagatcag cggccagccg gacgccctgc
     2461 ggcgagcggc ctcgg
//
LOCUS       DATDBZ010000423         4504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1357606, whole genome shotgun sequence.
ACCESSION   DATDBZ010000423 DATDBZ010000000
VERSION     DATDBZ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357606"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VIX62_10105"
     CDS             complement(<1..808)
                     /locus_tag="VIX62_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5860626.1"
                     /translation="MQTTTPTTPIAPNQPVDRQIFRRGMRLVGSYVRMHPKPFAASVA
                     GSVLFAFASLWLTTALARATDQVLKPAFEGTLDTSKVWIAVAGLMIGGVGRALGIMCR
                     RYYSGVAGERVMATLRTRVAERYRALSLQYHRETPTGELLAHMEADVKAAVDVFWPVP
                     FAVGVILLTVLALVALLLTDPWLALVGLIAFPSLAVMNRIFARRMEEPAQTAQERIGD
                     VSAVAHESIDGALVVKTLGREDAETRRLYEQADALRDARVRGGTIRATFEA"
     gene            complement(944..1522)
                     /locus_tag="VIX62_10110"
     CDS             complement(944..1522)
                     /locus_tag="VIX62_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860627.1"
                     /translation="MKRKTLLALSVIPLTFLLSSCFALQGFWIAANQIEAGGKATKAV
                     FEIRPATSTSDNAHQFFLVGVTDDNDLVAGKATWGTNGTFGGPYPLPVSAGLATVIGS
                     DCAQNGFNLADVTGVTWKGYVTPTSINDKNKVNKSVLVQVGLKAVDAATVTDQPVIGV
                     TGAWADDGDNVPEGTDTFLCTGVSQVSVNVRA"
     gene            1675..3408
                     /locus_tag="VIX62_10115"
     CDS             1675..3408
                     /locus_tag="VIX62_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY5860628.1"
                     /translation="MTDDRAWFASYPPDVPHSLAPYPKEPLYALLRSAVAGFPDRPAL
                     AFFGARMSYAALLKEVERFSAVLAGLGVKQGDRVGMILPNCPEYVIAFFACQRIGAVA
                     VGNNPLYTERELEHQIKDSGARVMIVLDQIYARFGEIRDVAGVQEVIGVKLNHYMAAP
                     IKWLAPLKFKSDAKKHGTPFPFIPEGHRVRWWADLMKQAGPVPPEATIADPDTEVAAL
                     VYTGGTTGLSKGAMLSHSNLVANARQASAWLNVVRQGEDGIVAALPFFHSFGTLVMDF
                     SMTKAAKLILLPRFEIDMALKAMAKEKPTLFPGVPRMYLALNQDPRTPKHDLGSLKAC
                     ISGAAPLPMAVAQEFERITGGAKVVEGYGLTECSPVTHANPLVGERKEGFIGLPLPDT
                     DVKLVDLDEPDREVARGERGEMCIRGPQVMLGYWNRPDESGLVIRNGWLHTGDVAIMD
                     EDGYFQIVDRIKDMILVSGFNVYPTEVEAVLYHHPKIFKCAVVGVPDDITGERVKAFI
                     VLKPGETATAEEITSWCRDPDQGLTGYRVPKEIEFRDELPETLIGKVLRRVLQEEERV
                     KREAAPGTSAP"
     gene            3405..3953
                     /locus_tag="VIX62_10120"
     CDS             3405..3953
                     /locus_tag="VIX62_10120"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY5860629.1"
                     /translation="MNPILWELGDGIVVRTLMPDDDVELFALVDRNRDRLRPWMAWEP
                     MTKGPGDTREFIERALASEHDREGNGIFLDGRMVGTAGMRVDAMDAKAEVGYWIDGDA
                     EGRGIVTRTCERFLALAFEELGLHRVELHAATGNGRSRAVAERLGMVEEGTGRENGRI
                     DGGFVDLVMFGILDREWRARRG"
     gene            3950..>4504
                     /locus_tag="VIX62_10125"
     CDS             3950..>4504
                     /locus_tag="VIX62_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5860630.1"
                     /translation="MSVPEPTGPVDAVLFDLYGTLVPEYSKADFYASVAAMAEAVGAE
                     VDTFRVAWDATARARQTGGFATVEDNVRAVCNELGLRPDDLAIARGLEPRAAMYRRWF
                     HPRVGAVEVLSTLRTRGYPIALVSMCAPDTPALWQTTELAALVDAEVFSSETGLRKPE
                     PAIYLEAASRLGVAPDRCVYVGDGA"
BASE COUNT          713 a         1499 c         1586 g          706 t
ORIGIN      
        1 cggcctcgaa ggtggccctg atcgtgccgc ctcgcacccg ggcgtcgcgc agggcgtcgg
       61 cctgttcgta caggcggcgg gtctcggcat cctcgcggcc cagcgtcttc accacgaggg
      121 cgccatcgat cgattcgtgc gccacggcgc tcacgtcacc gatgcgctcc tgtgcggtct
      181 gggccggctc ttccatccgc cgggcgaaga tccggttcat caccgcgagc gaggggaacg
      241 cgatgagccc caccagcgcg agccacggat cggtgagcag cagcgccacc agggccagca
      301 cggtcagcag gatcacgccc acggcgaacg gcaccggcca gaacacgtcg accgcggcct
      361 tcacgtcggc ctccatgtgc gccaggagct cgcccgtggg ggtctcgcgg tggtactgga
      421 gcgagagcgc gcgataccgc tccgccaccc gcgtccgcag ggtggccatg acccgctcgc
      481 ccgccacgcc cgagtagtac cggcggcaca tgatgcccag cgcccggccc acgccgccga
      541 tcatcaggcc ggcgacggcg atccagacct tcgaggtatc cagcgtgccc tcgaacgcgg
      601 gtttgaggac ctggtcggta gcgcgcgcca acgcggtggt cagccacagc gaggcgaacg
      661 cgaacagcac ggagccggcc accgaggccg cgaatggctt cgggtgcatg cgcacgtatg
      721 aacccaccaa ccgcatgccg cggcggaaga tctgccggtc gaccggctgg ttgggagcga
      781 tgggggttgt cggagtcgtg gtctgcatgg tggttcctca gagaacggag cggagcgcac
      841 acacgaacgc ccgctcacgc atggtacgaa ccgcgtgaac cggcgcccgt ggtacggggt
      901 ccgcggctga cgccgcggcc tcagatgagt tcgggttgga cggtcaggct ctgacgttga
      961 ccgacacctg actcacgccg gtgcacagga aggtgtcggt gccctcgggc acgttgtcgc
     1021 cgtcgtcagc ccaggcgccg gtgacgccga tcacgggttg atcggtcacg gtcgcagcat
     1081 cgaccgcctt gagcccgacc tgcaccagca cggacttgtt caccttgttc ttgtcgttga
     1141 tcgacgtcgg ggtgacgtac cccttccagg tcacgcccgt gacatcagcg aggttgaacc
     1201 cgttctgtgc gcagtcgctg ccgatcaccg tggccaatcc ggccgaaacg ggcagcgggt
     1261 agggaccgcc gaacgttccg ttggtgcccc aggtcgcctt gccggccacg aggtcgttgt
     1321 cgtcagtcac gccgaccagg aagaactggt gggcgttgtc gctcgtggag gtcgccgggc
     1381 ggatctcgaa gacggctttc gtcgccttcc caccggcctc gatctggttg gccgcgatcc
     1441 agaagccctg aagcgcgaag caagagctca acaagaacgt gagcgggatc acggacagcg
     1501 ctagcagtgt cttcctcttc atcgcggagg ctcccccttg agacgaacgg acgttggagc
     1561 gatggtcgca tcgcatgcgg ttcgacgcaa accccctgtg taaagtcgct ccgcggagcc
     1621 gatcggcggc ggtccggctt gcaggtggct cgggcggccg cgatgatgcg ccccatgacc
     1681 gatgatcgcg cctggttcgc ctcgtatcca cccgacgtgc cgcactcgct cgcgccttac
     1741 ccgaaggaac cgttgtatgc cttgctgcgc agcgcggttg cgggcttccc cgaccgtccg
     1801 gccctcgcgt tcttcggcgc gcggatgagc tacgcggcgc tgctcaagga ggtggagcgg
     1861 ttctcggcgg tcctcgccgg cctcggcgtc aagcagggtg atcgcgtggg catgatcctg
     1921 cccaactgcc ccgagtacgt gatcgcgttc ttcgcctgcc agcggatcgg tgcagtggcg
     1981 gtggggaaca acccgctgta caccgagcgc gagctcgagc accagatcaa ggactccggc
     2041 gcccgcgtca tgatcgtgct cgaccagatc tacgcgcggt tcggcgagat ccgggatgtc
     2101 gcgggcgtcc aggaggtcat cggcgtcaag ctgaatcact acatggcggc gccgatcaag
     2161 tggctggccc cgctgaagtt caagtccgat gcgaagaagc acggcacgcc gttccccttc
     2221 atccccgagg gccatcgggt gcgctggtgg gccgacctga tgaagcaggc cggtcccgtg
     2281 ccgcccgagg ccacgatcgc cgaccccgac accgaggtcg cggcgctggt ctacaccggc
     2341 ggcaccaccg ggttgtccaa gggcgccatg ctctcccact cgaacctggt ggcgaacgcc
     2401 cgccaggcct cggcgtggct caacgtcgtc cgccagggcg aggacgggat cgtggcggcg
     2461 ctgccgttct tccactcgtt cggcacgctg gtgatggatt tctcgatgac gaaagcggcc
     2521 aagctgatcc tgctcccgcg cttcgagatc gacatggcgc tgaaggccat ggcgaaggag
     2581 aagcccacgc tgttcccggg cgtgccgcgc atgtacctgg cgctcaacca ggaccctcgt
     2641 acgccgaagc acgacctggg gtcgttgaag gcctgtatct cgggcgcggc gccgctgcct
     2701 atggcggtgg cccaggagtt cgagcggatc accggtggtg ccaaggtggt cgaggggtac
     2761 ggcctcaccg agtgttctcc ggtcacgcac gccaatccgt tggtcggcga gcgcaaggag
     2821 gggttcatcg ggctgccgtt gcccgacacc gacgtgaagc tggtcgacct cgacgagccc
     2881 gaccgagagg tggcccgggg cgaacgcggc gagatgtgca tcaggggccc acaggtgatg
     2941 ctgggctact ggaaccggcc ggacgagagc ggcctggtga tccgcaacgg ctggctgcac
     3001 accggggacg tcgcgatcat ggacgaggac ggctacttcc agatcgtcga ccgcatcaag
     3061 gacatgatcc tggtgtcggg cttcaacgtg tatcccaccg aggtcgaggc ggtgctctac
     3121 caccacccga agatcttcaa gtgcgcggtg gtgggtgtgc ccgacgacat caccggcgaa
     3181 cgcgtgaagg cgttcatcgt gttgaagccc ggggagaccg ccaccgccga ggagatcacg
     3241 tcgtggtgtc gcgaccccga ccaggggctc accggctacc gcgtgcccaa ggagatcgag
     3301 ttccgcgatg agctccccga gacgctgatc ggcaaggtcc tgcggcgcgt gctgcaggag
     3361 gaagagcgcg tcaagcgcga ggccgcgccg ggcacgtccg ccccgtgaac ccgatcctgt
     3421 gggagctggg cgatgggatc gtggtccgca cgctcatgcc cgacgacgac gtcgagctct
     3481 tcgcgctggt cgaccgcaac cgcgaccgcc tacgcccatg gatggcgtgg gaacccatga
     3541 cgaagggtcc gggcgacacc cgcgagttca tcgaacgggc gttggcgtcc gagcacgacc
     3601 gtgagggcaa cgggatcttc ctggacgggc ggatggtcgg tacggccggc atgcgggtcg
     3661 acgcgatgga tgccaaggcc gaagtgggct attggatcga cggcgacgcc gagggacgcg
     3721 ggatcgtgac ccgaacgtgc gaacggttcc tggcgctcgc gttcgaggaa ctgggcctgc
     3781 accgggtcga gctccacgcc gccacgggca acgggcgcag tcgcgccgtc gctgaacgcc
     3841 tgggcatggt cgaagagggg acggggcggg agaacggccg gatcgacggt ggcttcgtcg
     3901 acctcgtgat gttcgggatc ctggatcgcg aatggcgagc gcgccgggga tgagcgtccc
     3961 cgagccgacg gggccggtcg acgcggtcct gttcgacctc tacggcacgt tggtgcccga
     4021 gtattccaag gccgacttct atgcgtcggt ggctgccatg gcggaggcgg tgggcgccga
     4081 ggtcgacacc ttccgggtgg cctgggacgc cacggcccgc gcgcggcaga ccggaggctt
     4141 cgccacggtc gaggacaacg tgcgtgcggt ctgcaacgag ttggggctgc gccccgacga
     4201 cctcgcgatc gcgcgtggcc tggaaccccg cgccgcgatg taccgccggt ggttccaccc
     4261 ccgcgtgggc gcggtcgagg tgctctccac gttgcggacc cgcgggtacc cgatcgccct
     4321 cgtcagcatg tgcgcgcccg acacgcccgc gttgtggcag accaccgagc tcgcggcgct
     4381 cgtcgacgcc gaggtgttct cgtccgagac ggggctgcgt aagcccgaac ccgccatcta
     4441 cctcgaggcg gcctcgcgcc tgggggtggc ccccgatcga tgcgtctacg tcggcgacgg
     4501 tgcc
//
LOCUS       DATDBZ010000424         3994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_962512, whole genome shotgun sequence.
ACCESSION   DATDBZ010000424 DATDBZ010000000
VERSION     DATDBZ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962512"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VIX62_10130"
     CDS             complement(<1..248)
                     /locus_tag="VIX62_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860631.1"
                     /translation="MTEADYAAYLPRATEAYAVELARNNLVSEEEGLARSRRSFETEL
                     PDGIHTPDQRLFIAEDAAGELVGYLWLARKPETDTVFI"
     gene            complement(284..1747)
                     /locus_tag="VIX62_10135"
     CDS             complement(284..1747)
                     /locus_tag="VIX62_10135"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY5860632.1"
                     /translation="MSDSSHRAARIPVTLIPGDGIGPECVTAACRIIEATGAPIDWED
                     AEAGAEVFKKGIASGVPQETMDSITRTRVALKGPLETPVGFGEKSANVTLRKLFETYA
                     NVRPVREMPGVTTPYSGRGIDLVMVRENVEDLYAGIEYMQTPGVAQALKLISVKGSEK
                     IARFAFEYARSEGRSSVACATKANIMKFSEGTLKRVFESVAPDYPEIESWHVIVDNAA
                     HQLVKKPEQYEVIVTTNMNGDILSDLSSALIGGLGFAPSANIGNEVAIFEAVHGSAPK
                     YAGKNVINPTAVILSSVLMLRSLGLFEEASAIEHAVFVTLESGTLTGDVVGYDAGVST
                     TDYTDAIIGNLGNKTDRWTIREVRPLKLPELSPDPDYVKVSSRAVVGLDVFVESPMSA
                     EELGASLTELATGTRLELKMISSRGTKVYPPTGTITDTLDHWRCRFIIREDPGDLWDE
                     DVHQLLARISRRHTWMHTEKLQEFDGELGFTRDQGEN"
     gene            1917..3389
                     /locus_tag="VIX62_10140"
     CDS             1917..3389
                     /locus_tag="VIX62_10140"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5860633.1"
                     /translation="MRSRGVFVVVALVLAFAATAATCSGDDPTVQPTGSVAASTPTTP
                     SARQGSTIDPSMGFGNIDHLVFVVQENRSFDHYFGTFPGADGIPRNDDGSFEVCVPDP
                     FAGGVCRTPYHDTNLYDAGGPHNSHASLIDVNGGRMDGYIQALESIGNSCLHDPSKFP
                     CPLTHPGPDDTPDVMGYHTAKEIPNYWAYARHYLLQDHMFAPTDSWTLPSHLAMVSGW
                     TARCPDLNDPMSCNTQVRKPDQWWPLKQGAKMPYAWGDITWMLGKAGVSWGYYVGPDS
                     CVDNPCKPQRNARSTNPIFNPLPGFQTVRQSGQLGNVAPYADFFNSINTGDLPSVSWV
                     VPTAGRSEHPGDELGNIATGQAWVTRVVNSIMQGPPEQWERTAIFLTWDDWGGFYDHV
                     LPPRVDEFGYGIRVPGIVISPWVDSDLDVDHQRYSFDSFLKLIEDRFIDGRRLDGENW
                     GWPDSRPTVREEVPGLGDLRDIFDFEQEPLPPLVLDPEPG"
     gene            3464..>3994
                     /locus_tag="VIX62_10145"
     CDS             3464..>3994
                     /locus_tag="VIX62_10145"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5860634.1"
                     /translation="MTRAALRILLPLIVLGIIAAAVPSSIKATASAAPPRGIDPAAGI
                     ENIDHFVFIVQENRSFDHYFGTFPGANGIPRFPNGRFKPCVPDPKGPCRRPYHDLNQF
                     DQGGPHGEQGSRISIDGGKMDGFVRSLRQIGNGCSKPENRDYYPCRKARNGPAGQPDV
                     MGFHTAREIPNYWKYAK"
BASE COUNT          637 a         1356 c         1305 g          696 t
ORIGIN      
        1 tagatgaaca cggtgtcggt ctcgggcttc cgcgcgagcc acaagtagcc gaccagttcg
       61 cccgcggcgt cctccgcgat gaagagccgc tggtcggggg tatggatccc gtcgggcagc
      121 tccgtctcga aggagcgtcg cgaccgcgcc aggccctcct cctcgctcac cagattgttc
      181 cgcgccagct cgaccgcgta cgcctcggtg gcacggggga ggtacgccgc atagtccgcc
      241 tccgtcatcg gacggagggt gacgaccggc ggtaccgcgg tcactagttc tcgccctggt
      301 cgcgggtgaa cccgagctcc ccgtcgaact cctgcagctt ctcggtgtgc atccaggtgt
      361 gacggcggct gatacgagcc agcaactggt gcacgtcctc atcccagagg tcgcccgggt
      421 cttcgcggat gatgaaccgg caccgccagt ggtccagcgt gtcggtgatc gtgccggtcg
      481 gcgggtagac cttcgtaccg cggctcgaga tcatcttcag ctccagccgc gtgccggtgg
      541 cgagctcggt gagcgacgcg cccagctcct cggccgacat cggtgactcc acgaacacat
      601 ccaggcccac gaccgcgcgc gagctcacct tcacgtagtc ggggtcgggc gagagctccg
      661 ggagcttcag cggccggacc tcgcggatcg tccatcggtc ggtcttgttg cccaggttcc
      721 cgatgatggc gtcggtgtag tcggtggtgg agacaccggc gtcgtagccc accacgtcac
      781 cggtcagcgt gcccgattcc agcgtcacga agaccgcgtg ctcgatcgcg ctcgcttctt
      841 cgaacaggcc gagggaacgc agcatcagca cgctcgacag gatcacggcg gtggggttga
      901 tcacgttctt gcccgcgtac ttgggcgccg acccgtgcac cgcttcgaag atcgccacct
      961 cgttgccgat gttggcgctc ggcgcgaagc ccagtccgcc gatcaatgcg ctcgagaggt
     1021 cggacaggat gtcgccgttc atgttcgtgg tgacgatcac ctcgtactgc tcgggcttct
     1081 tcacaagctg gtgggcggcg ttgtcgacga tcacatgcca cgattcgatc tcggggtagt
     1141 cgggcgccac cgattcgaag acgcgcttca gcgtgccttc ggagaacttc atgatgttgg
     1201 ccttcgtggc gcacgcgacg ctgctgcgac cctcgctgcg cgcgtactcg aacgcgaacc
     1261 gggcgatctt ctccgagccc ttcacgctga tcagcttcag cgcctgcgcg acccccgggg
     1321 tctgcatgta ttcgatgccg gcatagaggt cctcgacgtt ctcccggacc atcaccaggt
     1381 cgatgccccg gcccgagtac ggggtcgtga cgccgggcat ctcacgcacc ggtcggacgt
     1441 tcgcgtaggt ctcgaagagc ttgcggagcg tcacgttggc gctcttctcc ccgaacccca
     1501 cgggggtttc cagcggtccc ttgagcgcaa cgcgcgtgcg cgtgatcgag tccatcgtct
     1561 cctgcggcac gccggaggcg atgcccttct tgaagacctc ggcaccggcc tcggcgtctt
     1621 cccagtcgat gggtgcgccg gtggcctcga tgatccggca tgccgcggtc acgcactccg
     1681 gaccgatgcc gtcgcccggg atcagggtga ccgggatccg tgctgctcgg tgactgctgt
     1741 cgctcattcg gggctcaact ccttgcggtg gtgatgggcg tcgatggtgg cggtcgtatc
     1801 gatggttccg gcgctcggcc ggagccgcgt gcgggtcatc gtagcggcgg gtgctggttc
     1861 tcgcgtccgg gatgcgttgt tggacacccc catatccgcc gggtaccgtc gcgcggatga
     1921 gatcccgggg cgtgttcgtc gtggtcgcgc tcgtcctggc gttcgcggcg accgccgcga
     1981 cctgttccgg cgacgacccg accgtgcagc ccacggggtc ggtcgccgcc tccacgccca
     2041 ccacgccgtc ggccaggcag ggttcgacga tcgacccgtc gatgggcttc ggcaacatcg
     2101 accacctggt gttcgtggtg caggagaacc ggtccttcga ccactacttc ggcaccttcc
     2161 cgggggccga cggcatcccg cgcaacgatg acggttcctt cgaagtgtgc gtgcccgatc
     2221 ccttcgcggg tggcgtgtgc cggacgccgt accacgacac gaacctgtac gacgccggag
     2281 gaccgcacaa ctcccacgca tccctgatcg atgtgaacgg cgggcgcatg gacggctaca
     2341 tccaggcact cgagtcgatc gggaactcgt gcctgcacga cccctcgaag ttcccctgcc
     2401 cgctcacgca ccccggcccc gacgacacgc ctgacgtgat gggctaccac accgccaagg
     2461 agatcccgaa ctactgggcg tacgcgcgcc actacctgct ccaggaccac atgttcgcgc
     2521 ccaccgactc ctggacgctt ccctcgcacc tggcaatggt gtcgggctgg acggcgcgct
     2581 gtcccgatct gaacgacccg atgagctgca acacccaggt ccgcaagccc gaccagtggt
     2641 ggccactgaa gcagggcgcg aagatgccgt acgcatgggg cgacatcacg tggatgctgg
     2701 gtaaggcggg cgtgagctgg ggctactacg tgggtcctga cagctgcgtc gacaacccgt
     2761 gcaagcccca gcggaacgct cgttccacga acccgatctt caacccgctg cccgggttcc
     2821 agacggtgcg acagagcgga cagttgggta acgtggcgcc gtatgcggac ttcttcaact
     2881 cgatcaacac gggcgacctc ccgtccgtgt cctgggtggt gcccacggcc ggccggagcg
     2941 agcacccggg cgacgaactg gggaacatcg ccaccggcca ggcgtgggtc acacgcgtcg
     3001 tcaactcgat catgcaggga ccgcccgagc agtgggaacg cacagcgatc ttcctcacgt
     3061 gggacgactg gggcggcttc tacgaccacg tgctgccacc gagggtggat gagttcggct
     3121 acggcatccg tgtccccggc atcgtgatca gtccatgggt cgacagtgac ctggacgtgg
     3181 accaccagcg atattcgttc gattcgttct tgaagctgat cgaggaccgg ttcatcgacg
     3241 ggcgacggct cgacggcgag aactggggat ggccggactc gcggcccacc gtgcgcgagg
     3301 aggtgcccgg tctcggagac ctgcgcgaca tcttcgactt cgagcaggaa ccgctgccgc
     3361 ccctcgtgct ggatccagaa ccgggttgat cgatggcgat cctgtgtcag ggagcgagcg
     3421 gctccctgag cgggccgggg gagcgggtag catgcgcgat tccatgaccc gcgccgccct
     3481 ccgcatcctg ctgccgctca tcgtcctggg catcatcgcc gcagccgtgc cgtcatcgat
     3541 caaggccacc gcctccgcgg cgcccccgag aggcatcgac ccggcggcgg gcatcgagaa
     3601 catcgaccac ttcgtgttca tcgtgcagga gaaccgctcg ttcgatcact acttcgggac
     3661 gttccccggt gccaacggca tccctcgatt ccccaacggt cgcttcaagc cgtgtgtgcc
     3721 cgatccgaag ggtccgtgtc gcaggcccta ccacgacctg aaccagttcg atcagggcgg
     3781 accgcacggc gaacagggct cgaggatctc gatcgacggc ggcaagatgg acgggttcgt
     3841 acgttcgctg cggcagatcg gcaacggttg ctccaagccc gagaaccgtg actactaccc
     3901 gtgcaggaag gcgaggaacg ggccggccgg ccagcccgac gtcatgggct tccacacggc
     3961 gcgggagatc ccgaactact ggaagtacgc caag
//
LOCUS       DATDBZ010000425         6414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_766252, whole genome shotgun sequence.
ACCESSION   DATDBZ010000425 DATDBZ010000000
VERSION     DATDBZ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766252"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..744)
                     /locus_tag="VIX62_10150"
     CDS             complement(52..744)
                     /locus_tag="VIX62_10150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5860635.1"
                     /translation="MSTEDRRLYLIRHARPDYDSSDRTDTPRGEQYDPPLGEVGIEQA
                     RLLADRLQLLPRPAAVYTSTLARARQTAHAYADRVGIDVIERDDLCEWFGGEWEEKDF
                     EEIFAEHPEAIELFRNQNPAWHLAPGSEAGEAFQDRCVTAVEDIIATHPAGDVIVVAH
                     GGVINAYFAHILKIADQDMFFLPENTSLNTVVIRGDERLAWFLSDASHLTDPGWFAPR
                     LAADGDAVEAGA"
     gene            complement(741..1139)
                     /locus_tag="VIX62_10155"
     CDS             complement(741..1139)
                     /locus_tag="VIX62_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860636.1"
                     /translation="MTDEVRARMWEFGGRHYARILDASAFIEVTGVSRAECLAQLRQT
                     TGDEVTLLVEVVPTVVGVAEAAEIMGWDKRRVITYIDRGSFPEPITSLASGRIWLRED
                     VEGYATEWRASHASRRVANQATKPKGTPAL"
     gene            complement(1189..1356)
                     /locus_tag="VIX62_10160"
     CDS             complement(1189..1356)
                     /locus_tag="VIX62_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860637.1"
                     /translation="MQPTSSTGKEAPGVACIPVSESKRLPCDLCGHPMEPEHAHYRCP
                     SCHYILPCCGW"
     gene            complement(1383..1673)
                     /locus_tag="VIX62_10165"
     CDS             complement(1383..1673)
                     /locus_tag="VIX62_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860638.1"
                     /translation="MPRKNRRDPSYFEAPEAPPARADGPTWAQAPGYEVRHVGGQKAY
                     RCPGCDHTIRAGLWHLVVVPEGDVDARRHWHTECWRSELRRQGLYRPPPAGS"
     gene            complement(1673..2350)
                     /locus_tag="VIX62_10170"
     CDS             complement(1673..2350)
                     /locus_tag="VIX62_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018777512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY5860639.1"
                     /translation="MPTTEERLSFAVPDKGEVSGVYARPAPAFATIVVAHGAGAGMDH
                     PFMSGFTRAMNDEGAATLRFNFPYIERGSRSPDRAPVAIDAWRAAFEVAASRAKAGEP
                     VWASGKSFGGRMASMAVSEGMGAAGLVFLGYPLHPPGKPERVRDEHLYGIEVPMLFIE
                     GTRDPFATAEVLDPVLAKLGDRATLYPVEGGGHSFETRGGKRDPREVAAELAPVAAAF
                     MREHAGP"
     gene            2371..3093
                     /locus_tag="VIX62_10175"
     CDS             2371..3093
                     /locus_tag="VIX62_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY5860640.1"
                     /translation="MTGSFYLGTSGFAYDGWKHDVFYPEGLKNREMLSYYSSQLNSVE
                     INYTFRRFPTKNSLTTWREQAADGFVFTLKANQRITHSRRLKDADDDVRDFLDLAKLL
                     GDRLGCVLFQCPPNLQYDRTLIEQFVGFLPPGPPRFAMEFRHPSWVEARDLLRDQGIA
                     WCVAQTDDKDPEAGDLSWVPAGYLRLRKTEYTDEELQEWAGRIRSALDEGADVFTYFK
                     HEDEGASPKMAKRLEDLLDRNA"
     gene            3137..4036
                     /locus_tag="VIX62_10180"
     CDS             3137..4036
                     /locus_tag="VIX62_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5860641.1"
                     /translation="MNDLRVGIQLPEVERDVRWPEYLAMARTAEAVGFDSIWVGDHYL
                     YRGDGRPERGPWEAWTLLAALAAVTERVRLGPMVACLNFHEPAVLAKLAATVDEVSGG
                     RLVFGMGAGWNRTEFEAFGIPYDHRASRFEEAFDIVRRLLAGERVSVRGQWHTVDEAV
                     LLPAPSNRATLMVGSSGERVLAATLPYVDVWNTWFDWYGNTAEGFAARTAQIDAACER
                     AKRDPSTLVRSACVLVQLGDIGVERPAAPDVTPLSGSLDEIAHGMRAMADAGADEVIV
                     VCDPITEPSIAQLGEMLSILRAP"
     gene            complement(4122..4715)
                     /locus_tag="VIX62_10185"
     CDS             complement(4122..4715)
                     /locus_tag="VIX62_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860642.1"
                     /translation="MNRKTWDRLVSTAALVIAVVLVFAGGAAVYGGNFGRDNVTDRLA
                     PEKVVFPPLEAMTPQEQTEVGGFAGEAVTTGPQAEAFARYIQGHLAEVNEGQTYSETS
                     AAAREEGLDPKVAADLQIKADTLFKGETLRAILLNAYGWWTVATIAVWAGWGMVTAGI
                     VLLILSILGFVHARKTSERVVVTEERTQDREYTPAGV"
     gene            complement(4855..5097)
                     /locus_tag="VIX62_10190"
     CDS             complement(4855..5097)
                     /locus_tag="VIX62_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860643.1"
                     /translation="MSDPDEELPGPLDPDDEPSPTQPGGAILIGPVLGVIIGYVVFQL
                     LPEDTSILVGLGIALVVITLTTYITLEISRRRARRD"
     gene            complement(5107..>6414)
                     /locus_tag="VIX62_10195"
     CDS             complement(5107..>6414)
                     /locus_tag="VIX62_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY5860644.1"
                     /translation="ANVRCRPQRDPYHRFTVDVHLTTAWSSMQRMLNAAGGDDPVERE
                     MVKQVDDRDGALLGALLHDIGKTGEGGHVPIGARIAAETLERIGVEPSTRELASFMVR
                     EHLLLPDTATRRDLTDENLILDVAATVGTTERLAALYLLAKADAEATGPAAWTPWRQT
                     LIRELVGRVQRVFDRGDMGTELAGRLTERVDRLRDLLVDEPDDVVERFVFRMPRAYFL
                     AVEPAAAARHLATIAPDLGSGEVRTAAVATERPGSHELLVVAADRSGLLSWIAGSLAL
                     AGLSIQAAQAFTTEDGAAVDLFEVHGVFEEEISEARWRAFRGTLRKAVAGQMSLAHRV
                     GEQRRRYPEPRSGLPVTVAVDNDASDFSTVIEVGAPDRPGLLYDITSVLADLQLDVHL
                     AKVATYTGRVIDAFYVRDALGGKITSPEQIAEVESAMRGRLEG"
BASE COUNT          978 a         2239 c         2160 g         1037 t
ORIGIN      
        1 ctcccttgcg gtctgcgttc ggtttcgcgc gagtataggc ggcaacgccg ttcaggcccc
       61 cgcctccacc gcatcgccgt cggcggcgag ccgcggggcg aaccacccag gatcggtgag
      121 gtgactcgcg tccgacagga accacgcgag gcgctcgtcg ccacggatca ccaccgtgtt
      181 gaggctggtg ttctccggca ggaagaacat gtcctggtcg gcgatcttca ggatgtgtgc
      241 gaagtaggcg ttgatcacgc ccccgtgtgc caccacgatg acgtcgccgg ccgggtgggt
      301 cgcgatgatg tcctccaccg cggtcacgca ccggtcctgg aaggcctccc cggcctcgct
      361 ccccggagcg aggtgccaag ccgggttctg gttccggaaa agctcgatcg cctcggggtg
      421 ctcggcgaag atctcctcga agtccttctc ctcccactcc ccgccgaacc actcacagag
      481 gtcgtcgcgc tcgatcacgt cgatgccgac acggtcggcg taggcgtgcg ccgtctgccg
      541 agcgcgcgcc agcgtggagg tgtagaccgc cgccggcctg gggagcagtt ggagccgatc
      601 ggccagcaag cgagcttgct cgatacccac ctcaccgagc ggcggatcgt actgctcgcc
      661 gcgaggcgtg tcggtccggt cgctcgagtc gtagtcgggg cgagcgtggc ggatcaggta
      721 gaggcgtcgg tcctcggtgc tcacagggca ggggttcctt tcggcttcgt ggcctggttc
      781 gcgactcggc gggatgcatg gctggcgcgc cattcggtgg catagccctc gacgtcctcg
      841 cgcagccaga tccgaccgga tgccagcgag gtgatgggtt cggggaacga accccgatcg
      901 atgtaggtga tcacgcgacg cttgtcccac cccatgatct cggccgcttc ggccaccccc
      961 accacggtcg ggaccacctc gaccaacagc gtcacctcgt caccggtggt ctggcggagc
     1021 tgggcgagac actcggcgcg gctgacgccc gtgacctcga tgaaggccga cgcatcgagg
     1081 atccgtgcgt agtgacgccc gccgaactcc cacatccggg cgcgcacctc gtcggtcatg
     1141 gcggcaggat agcgccgctg tatgacaccg tggcgcgatc cccgcggctc accagccgca
     1201 gcagggaagg atgtagtggc agctcgggca gcggtagtgg gcgtgctcgg gttccatcgg
     1261 gtgcccacac aggtcgcacg gcaggcgctt ggactcgctc accgggatgc acgcgacgcc
     1321 aggcgcctcc ttccccgtcg agctggtggg ctgcatgtcg ggcatcctag cccgggggtc
     1381 tgtcaggatc cggcaggcgg cggacgatag aggccttggc ggcgaagttc cgaccgccag
     1441 cactcggtgt gccagtgcct gcgggcgtcg acatcgccct cgggcaccac cacgaggtgc
     1501 cacaggccgg cccggatcgt gtgatcacag ccgggacacc ggtacgcctt ctgtccgccg
     1561 acgtggcgca cctcgtagcc cggcgcctgg gcccacgtcg gtccatcggc gcgagccggc
     1621 ggtgcctcgg gcgcctcgaa gtacgacggg tcgcggcggt tctttcgagg catcagggcc
     1681 ccgcgtgctc gcgcatgaac gcggcggcga cgggcgcgag ctcggcggcg acctcacgcg
     1741 ggtcccgctt gcccccgcgc gtttcgaagg agtggccgcc gccttcgacc gggtacagcg
     1801 tggcgcggtc gccgagcttg gccagcaccg ggtccagcac ctcggcggtg gcgaacggat
     1861 cgcgggtgcc ttcgatgaac agcatcggca cctcgatgcc gtacagatgc tcgtcgcgaa
     1921 cccgctccgg cttgcccggt gggtggagtg gataccccag gaacaccagg cctgccgccc
     1981 ccatgccctc gctcaccgcc atcgacgcca tcctcccgcc gaaggacttg ccgctcgccc
     2041 acaccggttc gcccgccttc gctctcgacg ctgcgacctc gaaggccgcc cgccaggcgt
     2101 cgatcgccac gggagcgcgg tcgggcgatc gcgagccgcg ctcgatgtag gggaagttga
     2161 accgcagcgt ggcggcgcct tcatcgttca tcgcgcgcgt gaacccgctc atgaaggggt
     2221 ggtccatgcc ggcgcccgcg ccgtgggcca ccacgatcgt ggcgaacgcc ggagcgggcc
     2281 gtgcatacac cccgctcacc tcacccttgt cgggcacggc gaacgacaag cgttcctcag
     2341 tcgtgggcat cacatgccat gatgcacgcc gtgacgggaa gcttctacct cgggacctca
     2401 ggattcgcct acgacggctg gaaacacgac gtgttctatc cggagggact gaagaaccgc
     2461 gagatgctct cctactactc ctcgcagctg aactccgtcg agatcaacta cacgttccgc
     2521 cgattcccca ccaagaactc cctcaccacc tggcgcgagc aggcggccga cggattcgtg
     2581 ttcaccttaa aggcgaacca gcggatcacg cactcacggc gattgaagga cgccgacgac
     2641 gacgtccgcg acttcctgga cctggcgaag ctgctcgggg accggctggg ctgcgtactg
     2701 ttccaatgcc cgccgaacct gcagtacgac cggacgctga tcgaacagtt cgtggggttc
     2761 ctgccgccgg gcccgccccg gttcgcgatg gagttccggc acccctcgtg ggtcgaggcg
     2821 cgcgacctgc tgcgcgacca gggcatcgcc tggtgcgtgg cccagaccga cgacaaggac
     2881 cccgaggcgg gagatctgtc gtgggtgcca gccgggtacc ttcgcctgcg caagacggag
     2941 tacacggacg aggagctgca ggagtgggcc ggaaggatcc ggtcggccct ggacgagggc
     3001 gccgacgtct tcacctactt caagcacgag gacgaaggcg cgagccccaa gatggcgaag
     3061 cgcttggaag acctgctcga tcgcaacgcg taacgcgtaa cgccgtctgc aggctcctgc
     3121 tgccacactg cccctcatga acgatctgcg agtcggcatc caactccccg aggtggaacg
     3181 cgacgtgcga tggcccgagt acctcgcgat ggcgcgcacc gccgaggcgg tgggcttcga
     3241 ctcgatctgg gtgggcgatc actacctgta tcgcggcgac ggccgccccg agcgtggacc
     3301 gtgggaggcc tggacgttgc tggccgccct cgccgccgtg accgagcggg tgcggctcgg
     3361 accgatggtc gcgtgcctga acttccacga acccgccgtg ctcgccaagc tggcggccac
     3421 ggtcgacgag gtcagcggcg gccggctggt cttcggcatg ggcgcagggt ggaaccggac
     3481 cgagttcgag gcgttcggga tcccgtacga ccaccgggcg tcgcgcttcg aggaggcctt
     3541 cgacatcgtt cggcgcctgc tcgccggtga acgggtgtcg gtccgcgggc agtggcacac
     3601 ggtcgatgag gcggtgctgt tgccggcacc gtccaaccgg gcaaccctga tggtgggctc
     3661 gtccggcgag cgcgtgcttg cggcgacctt gccctacgtc gacgtctgga acacctggtt
     3721 cgactggtac ggcaacacgg cggaggggtt cgcggcgcga acggcccaga tcgacgcggc
     3781 gtgtgaacgt gcgaagcgcg acccgtcaac gctggtgcgc agcgcgtgtg tcctcgtcca
     3841 actcggtgac atcggcgttg aacggccggc cgcccccgat gtgacgccac tgagtggctc
     3901 gctcgacgag atcgcccacg gcatgcgggc catggccgac gccggtgccg acgaggtcat
     3961 cgtcgtgtgc gacccgatca cggagccttc gatcgctcag ctgggcgaga tgctgtcgat
     4021 ccttcgcgct ccgtagggtc gagcccgact tcgaccgctg gggtagaaag aacggccgcc
     4081 cctgagggac ggccgttctc gtgatcaggg ttcgtacgga atcagacgcc ggccggagtg
     4141 tattcccggt cctgggtcct ttcctccgtc accacgaccc gttcgctcgt cttcctggcg
     4201 tggacgaagc ccaggatcga caggatcagc aggacgatcc ccgcggtcac catgccccag
     4261 cctgcccaga cggcgatcgt cgcgacggtc caccagccgt aggcgttcag caggatggct
     4321 cgcagcgtct cgcccttgaa cagggtgtcg gccttgatct gcaggtctgc agcgaccttc
     4381 ggatcgagcc cctcttcacg cgctgccgca ctcgtctccg agtaggtctg gccttcgttg
     4441 acctcggcca ggtgcccctg gatgtagcgt gcgaacgctt ctgcctgtgg tccggtcgtg
     4501 accgcttctc ccgcgaatcc gccgacctcg gtttgctcct gcggcgtcat cgcctcgagc
     4561 ggcgggaaga ccaccttctc cggtgccaac cggtcggtga cattgtcccg gccgaagtta
     4621 ccgccgtaga ccgcggcgcc accggcgaag accagcacga cggcgatcac cagtgctgcg
     4681 gtgctgacca gacgatccca cgtcttcctg ttcatgacgt gctcctttcc tcgttgcaac
     4741 cattcatcac tcctccaggt tgccccatgg tcaggtcgca ggcggaggcc gttgggaacc
     4801 gcttcggcgg gactttgccc ggtcctgggg ggccgttcga cccccggagg gtgcctagtc
     4861 tcgacgggcg cgtcgacgcg agatctcgag cgtgatgtac gtcgtgaggg tgatcaccac
     4921 gagcgcgatc cccaggccca cgaggatcga cgtgtcctcc ggcagcagct ggaagaccac
     4981 gtacccgatg atcacaccca gcacgggacc gatcaggatc gcaccgccag gctgagtggg
     5041 cgacggttcg tcatcgggat cgaggggacc ggggagttcc tcgtcggggt cgctcatgcc
     5101 gcgagcttag ccctccagcc ggcctcgcat ggcgctttcc acttccgcga tctgctcagg
     5161 gctcgtgatc ttgccaccca gggcgtctcg gacgtagaac gcatcgatca cccggccggt
     5221 gtacgtggcg accttggcga ggtgcacgtc gagttgcagg tcggcgagca cgctggtgat
     5281 gtcgtagagc aaccccggcc gatccggcgc gccgacctcg atcaccgtgg agaagtccga
     5341 ggcgtcgttg tccacagcca ccgtcacggg caggcccgat cgaggctcgg ggtagcgacg
     5401 gcgctgctcc cccacgcggt gcgccaagct catctgcccc gccacggcct tgcgcagggt
     5461 cccgcggaag gcacgccatc gcgcttccga gatctcctcc tcgaagaccc cgtggacctc
     5521 gaacaggtcg accgccgcac cgtcctcggt ggtgaacgcc tgcgccgcct ggatcgagag
     5581 gcccgccagg gccagcgagc cggcgatcca cgagagcaac cccgaccggt cggcggccac
     5641 caccaggagc tcgtgtgacc ctggtcgctc cgttgccacg gcggcggtcc ggacctctcc
     5701 cgagcccagg tcgggcgcga tcgtggcgag gtgtcgggca gccgccgccg gttccaccgc
     5761 caagaagtac gcgcggggca tgcggaacac gaaccgctcc acgacgtcat cgggttcgtc
     5821 caccaacagg tcgcgcaacc ggtccacgcg ctcagtgagc cggcccgcca gttcggtccc
     5881 catgtcgccg cggtcgaaca cgcgctggac gcgtcccacg agctcgcgga tcaacgtctg
     5941 ccgccacggc gtccacgcgg ccggtccggt cgcttcggcg tcggctttgg ccaacagata
     6001 cagcgccgcg aggcgttccg tggttcccac cgtggccgcc acgtccagga tcaggttctc
     6061 gtccgtgagg tcgcgccggg tggcggtgtc gggcagcagc agatgttccc gcaccatgaa
     6121 cgacgcgagc tcgcgggtcg acggctcgac cccgatccgc tcgagcgtct cggcggcgat
     6181 ccgggcgccg atcggtacgt gcccgccctc gcccgtcttg ccgatatcgt gcaggagcgc
     6241 gccgagcagc gccccgtcac gatcgtcaac ctgcttgacc atctcccgct cgaccggatc
     6301 gtcgccgccg gcggcattca gcatccgctg catcgacgac cacgccgtgg tgaggtgcac
     6361 atcgacggtg aaccggtggt aggggtcgcg ttgcggacgg catcgcacgt tcgc
//
LOCUS       DATDBZ010000426         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2801538, whole genome shotgun sequence.
ACCESSION   DATDBZ010000426 DATDBZ010000000
VERSION     DATDBZ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2801538"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VIX62_10200"
     CDS             complement(<1..1160)
                     /locus_tag="VIX62_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY5860645.1"
                     /translation="MTTTSDPTTAGPGTARSPGLSYQQLLDTDTHEVPDILRDESPRF
                     LGDADVPIAHYISREWHELEKQRLWSRVWQFACREEHIPRVGDHTVYDIAGQSYVIMR
                     SAPDTIKAFPNACLHRGRQLKQYDGNCSEIRCPFHGFAWNLDGTLKDVPAHWDLPHVT
                     AERFELPEVQVGTWDGFVFINPDPNAEPLADFVKELAAHFDRWNLRDRYVQAHVSKVI
                     RANWKITQEAFSEAFHVNATHPQILPYIGDTNSQVDVWENCSRVITPAATPSPLLDWA
                     PDDETILRYALDVRLDEELFIKVEEGGTARSAMANATRDRWRPVVGELIDHWSDAEMV
                     DNLDYTLFPNFHPWGAFNRIVYRFRPNGDDHRSSIMEVLFLAPFKGERPPPAA"
     gene            complement(1174..>2149)
                     /locus_tag="VIX62_10205"
     CDS             complement(1174..>2149)
                     /locus_tag="VIX62_10205"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY5860646.1"
                     /translation="ERLTFRNAINQAMDQALAGDDRVFVLGEDVADSAGGGVFHVTDG
                     LSAKHGEDRVRNTPISEEAIMGASIGAAIAGLRPLPEIMFMDFLGVAFDQLANHAAKL
                     RYMSGGRTPVPLTGRVNMVGGTPIGAQHSQSLEALLMHTPGLKVVFPSTPYDAKGLLL
                     ACIEDEDPCVFIESTALFMKRGMVPTAPYTIPIGRAAVRRDGDDITIISYGRLMAEAL
                     KAAEQLGEEGVSAEVIDLRSLAPLDMDTLLTSVAKTTRAVVVHEAVRTCGAGAEIAAR
                     LHDELHDTLSGPVRRVTAPDSSVPAPAGLVAVFYPNASSILAACRTLV"
BASE COUNT          331 a          688 c          769 g          361 t
ORIGIN      
        1 gccgcaggcg gcggtcgctc acctttgaac ggagccagga acagcacctc catgatcgac
       61 gagcgatggt cgtcgccatt ggggcggaac cggtagacga tgcggttgaa cgcaccccag
      121 gggtggaagt tcgggaacag cgtgtagtcg aggttgtcga ccatctcggc gtcggaccag
      181 tgatcgatca gctcgcccac gaccggtcgc cagcggtcgc gggtggcgtt cgccatcgcc
      241 gagcgcgccg tcccgccctc ctcgaccttg atgaacagtt cctcgtcgag gcgcacgtcg
      301 agggcgtagc gcaggatcgt ctcgtcgtcc ggcgcccagt cgagcagggg gctgggcgtc
      361 gcggccggcg tgatgacccg gctgcagttc tcccacacgt cgacctggct gttggtgtcg
      421 ccgatgtacg ggaggatctg cggatgggtg gcgttgacgt ggaacgcctc gctgaacgcc
      481 tcctgggtga tcttccagtt ggccctgatc accttcgaca cgtgggcctg gacgtagcgg
      541 tcgcggaggt tccagcgatc gaagtgagca gccagctcct tgacgaagtc ggcgagtggc
      601 tcggcgttcg gatcggggtt gatgaagacg aacccgtccc acgtgccgac ctggacctcg
      661 ggcagctcga accgctcggc ggtgacgtgc gggaggtccc agtgggcggg cacatccttc
      721 aacgtgccgt cgaggttcca ggcaaagccg tggaagggac agcggatctc ggagcagttg
      781 ccgtcgtact gcttgagctg gcggccccgg tggaggcagg cgttggggaa ggccttgatg
      841 gtgtcgggag cggaccgcat gatcacgtac gactggccgg cgatgtcgta caccgtgtga
      901 tcgccgacgc gaggaatgtg ctcttcccgg caggcgaact gccacacccg cgaccagagc
      961 cgctgcttct ccagctcgtg ccactcgcgg gagatgtagt gagcgatcgg gacatcggcg
     1021 tcgcccagga agcgcggcga ctcgtcacgc aggatgtcag ggacctcgtg ggtgtcggtg
     1081 tcgagcaact gctgatagct gagaccgggg gaccgcgccg ttcccggccc tgcggtcgtt
     1141 ggatcggatg tggtggtcac gtgacggcct ccttcatacg agcgtccgac aggcggcgag
     1201 gatggaactg gcgttggggt agaacaccgc aacgagcccg gcgggggccg gtacggacga
     1261 gtccggggcg gtcacacggc gcaccggccc gctgagcgtg tcgtgcagct cgtcgtgcaa
     1321 tcgcgccgcg atctccgccc ctgcaccgca cgtgcgcacg gcctcgtgga cgaccaccgc
     1381 ccgggtcgtc ttggcgaccg acgtcaatag cgtgtccatg tcgagcgggg cgagcgaccg
     1441 caggtcgatg acctccgccg agacaccctc ctcgcccagt tgctcggccg ccttcagcgc
     1501 ctctgccatc aggcgcccgt agctgatgat cgtgatgtcg tcgccgtcgc ggcgcacggc
     1561 agcgcgtccg atcgggatcg tgtagggcgc ggtgggcacc atgccgcgct tcatgaacag
     1621 cgcggtgctc tcgatgaaca cgcacggatc ttcatcctcg atgcaggcca gcaacaggcc
     1681 cttcgcgtca tagggcgtcg acgggaacac caccttcagc ccgggggtgt gcatcaggag
     1741 cgcctccagc gactggctgt gctgggctcc gatcggggtg ccgccgacca tgttgacccg
     1801 accggtcaac ggcaccggcg tgcgtccgcc ggacatgtag cggagcttgg cggcgtggtt
     1861 ggcgagctgg tcgaacgcca cgccgaggaa gtccatgaac atgatctcgg gcagcgggcg
     1921 caggccggcg atggcggcgc cgatgctcgc tcccatgatc gcctcctcgg agatcggggt
     1981 gttgcggacc cggtcctcgc cgtgcttcgc ggagagcccg tcggtcacgt ggaacactcc
     2041 gccgccggcc gagtcggcga cgtcctcccc cagcacgaac acgcggtcgt cgccggccag
     2101 cgcctggtcc atcgcctggt tgatggcgtt gcggaaggtg agtcgctcg
//
LOCUS       DATDBZ010000427         9227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2801606, whole genome shotgun sequence.
ACCESSION   DATDBZ010000427 DATDBZ010000000
VERSION     DATDBZ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9227
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2801606"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..646
                     /locus_tag="VIX62_10210"
     CDS             104..646
                     /locus_tag="VIX62_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY5860647.1"
                     /translation="MDPLTGMLLIANSPLFDPNFRRTVVLIGHHDEEGAIGVVLNRTL
                     EVTVREAVPPLGGLVTEDEPWHVGGPVQPDSVVVVADFVDPSRAEILAMGSIGFLPPE
                     ADEDIVTAIRRARVFAGYSGWGAGQLEAELAEDSWLVEPAIPDDVFHPEPGRLWADVL
                     RRKGRDFDMLRLMPDDPAMN"
     gene            694..1656
                     /locus_tag="VIX62_10215"
     CDS             694..1656
                     /locus_tag="VIX62_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHO alpha subunit C-terminal catalytic
                     domain-containing protein"
                     /protein_id="HEY5860648.1"
                     /translation="MDRTPAPLDPEAVERCTRPFGTSFAMPGAAHTSPEVFAWEQEHF
                     FAPSWVCLGRAGTHRQVAGQVERAGWAFARRDADGIAFEDQFGNLWDHLAPWTPERLV
                     VGARHSYEIAANWKLIHENYQECYHCSEIHPALCKVTPPDSGYSIGLLGLFVAGPMDL
                     RDDVETMSLDGRSGGVPIPGLTGNQLREVGYFGVFPNLLISPHPDYVLTHRIEALAPD
                     RSFIECEWLFPPEAWDRPGFDPAYAVDFWDITNREDWVACESLQRSAASRGYRPGPLS
                     TAWEAGPYLQTRMIALGYQEGAVTRWPERPKQAERPRHELLRGA"
     gene            1653..2432
                     /locus_tag="VIX62_10220"
     CDS             1653..2432
                     /locus_tag="VIX62_10220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5860649.1"
                     /translation="MSGASVSFDRAAAYYDETRVTDPDALRTIVDLLASVMPGRGPVI
                     ELGVGTGQIALPLADRGVDVTGLDLSAEMMTVLRAKPGGPALPLIQGDATRLPIADGS
                     LGGAYMRWVLHLIPDWMGVLRELDRTAVTDGAVAIEPGGFSGPFRETYLRYREILGDA
                     VVALGLTAVDRDEQLDEGFALVGWALQDEVPVMYERTTTLQEVFDEVPSKRWSWTWRV
                     PDDELARATDEVRAWATEHFGDLDRPIPSEHVRWRVYRRAA"
     gene            2429..3208
                     /locus_tag="VIX62_10225"
     CDS             2429..3208
                     /locus_tag="VIX62_10225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5860650.1"
                     /translation="MTDSIAFDRAAEYYDATRGLSEEGVRLTTEALAEVFLGAGPILE
                     VGVGTGQVALPLHDAGVRVMGVDLSRPMLAKLRAKTGGARPFPLVEGDATSLSFADDA
                     FAGAYLRWVLHLIPDWRAAVREIARILSPGSAFVAALGAYGGYRQEMQARFAEITGIS
                     TEPAGLAWNGWDELDAELASLGLQKLPDIGFTDQDRDDLEHFMQAIEGNRFSWTWAVP
                     DAATREAAAAEVRHWAEERWGPLSEIPPETFDWRFAVYRLP"
     gene            complement(3215..3628)
                     /locus_tag="VIX62_10230"
     CDS             complement(3215..3628)
                     /locus_tag="VIX62_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860651.1"
                     /translation="MTSDQPGMRDPHDPDDQYSPSEGPSPSNDDADSGESLESYISEM
                     DHSLGVDDHTTTSEQMAGDTIDDRTRREQPQNDRRHKHLDVTDDSDVEALDHEAALVG
                     ESAETFEPDAPEVDAMHVVDDLPGAVDHPDDYVDE"
     gene            3752..4678
                     /locus_tag="VIX62_10235"
     CDS             3752..4678
                     /locus_tag="VIX62_10235"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY5860652.1"
                     /translation="MPITEVEKIWMDGELVDWRDAQVHVLSHALHYGSGVFEGIRAYE
                     TVRGPAVWHLDEHLKRLYRSAKLYHLDIPFSPEALKDATKEVIRANDLQSCYIRPLAF
                     RGYGEIGVNPLESPVNVTIAVWPWGAYLGEDALEQGVRVKVSSWRRNAQNSLPAAAKA
                     TGQYINSVLAKVESLKAGYDEAIMLNDHGYVTDGSGENIFIVRKGVLFTPPTQAGSLD
                     GITRETTMALARDAGYEVREENLVRTDLYNAEECFFTGTAAEITPIREVDDREVGEGH
                     RGPITKELQSAFFAATKGENPKYPDWLTYVND"
     gene            complement(4685..6070)
                     /locus_tag="VIX62_10240"
     CDS             complement(4685..6070)
                     /locus_tag="VIX62_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860653.1"
                     /translation="MAHDRDDIADPRLSMPEVDPGTEFELFFELEFERVFGAAYLATG
                     SRRDADHLAHVALLRTWDRWARAREMERPSAFPIRVALDPSRMPWRRAVVSARGRLHG
                     ERRLEPFEDVTIDDRVRQVLETMPWRRRAAITLTGSLHLTAGEAGRALRMRARRIREE
                     VAAATTDLSDTVADDEEPTEPAAVLDTVRLAAAPASGTFARHQSLRRRTRDRRRMATA
                     VCALSLAALVIFLGTRGSTSGAPADASPTPVQGALDAGTYRFPDISAAASVTLPEGWR
                     AGDSIWGSDGRGFAAVTTGPAGGSVSVAVFDLAHLNPVDPVGHVYAQTPARLRQWYDG
                     FDAEFEREVRPRLRSAIAGVAGRWQPGRPLAWILGQVPRGAIEVSEPIIAGRLGTLAS
                     FVNTGPSSALFGITGVGTISLRPDVTYTFWVPTDPDAFGTPIMVGIAREAGSIPTAGE
                     WKVVGSLAFDG"
     gene            6185..8710
                     /locus_tag="VIX62_10245"
     CDS             6185..8710
                     /locus_tag="VIX62_10245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEY5860654.1"
                     /translation="MQDRYRLPRSVIPSRYDLTIEPDLEAATFSGTEAVTVEVHERID
                     EIVINALALEIDNGWLSNATGTRIEVSGIAFDEELQRATLGLADPADPGSWTLYLAFR
                     GELNDQLHGFYRSTYADDDGDTHTIATTQFEAADARRAFPCWDEPDLKAVFAITLIVP
                     DGLAAISNGPEVEREDLGNDRIRVRFAETMSMSTYLVAFVVGRLECSDPVDADGVPIR
                     VVYVPGKGHLTKFALDAAVFSMWFFTDYYGLPYPDRKVDMIALPDFAQGAMENTGCIT
                     YRESLLLVDPEQATQPELENIADVVAHELAHQWFGNLVTMRWWNGIWLNEAFATFMAL
                     VTVDAWRPDWERWSSFARACTSAKEVDALHATRAIEYPVHSPDDTAGMFDVLTYQKGA
                     ATLRLLDRYLGGDEFRDGIRLYLQRHAYGNTETHDLWNALEEASGEPVRRIMDQWIWQ
                     GGYPLLTVSPDDEGVRIAQRRFLADGADDVTTWDVPLRVRELGGEERSVLVPAGGVSS
                     PADPDAAMVANAGASSFVRVRYDGDLLDRLTGRLSEVSPLERYGLVDDHWAAVSSGAA
                     DASAFVRFAAGFQDEDDLAVWQALMAGLGWCDRFVEGEARERFRGFVRGLVAPAMDRL
                     GWEAREEDADLTRALRGALLQGLGVLGADPNAEAAAREFEGEARAGKPVDPALAAAAV
                     NVVAVAGDTDDYERFWEAYRDAPTPQEQLRYLIALPLFRDPALMDRTLAATLGDDIRS
                     QNAPFVVAFAGTNRDLGPRSWTFLTEHWDDLVSRFPSSLTIRMVDGVRFLSRPEDVAA
                     AAAFFERHPIPQSAKTLQQMLERQRIAAALRLRATPDLTAHFS"
     gene            complement(8721..9113)
                     /locus_tag="VIX62_10250"
     CDS             complement(8721..9113)
                     /locus_tag="VIX62_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY5860655.1"
                     /translation="MALLLASAVAVPACGSARAAEDLRTVPITIHHSAFDPDELDVAP
                     GETVRFVVENTDPIDHEFLVGNEQVQAVHELGTEAHHAPKPGEMSVPALTTRVTTYTF
                     PTGHGSTIFGCHLPGHYAYGMRGAIRIG"
BASE COUNT         1396 a         3183 c         3186 g         1462 t
ORIGIN      
        1 tggaccatgg cccacacgct gtcatggggg ttcgatgccc acggggcgat gcccgaacac
       61 gtcgagaccg ctacatggct gctcgacgcg agatgatgcc cgggtggacc cgctcaccgg
      121 catgctcctg atcgcgaact caccactgtt cgacccgaac ttccgtcgca ccgtggtgct
      181 catcggccac cacgacgagg agggggcgat cggcgtggta ctcaaccgga cgttggaggt
      241 caccgtccgc gaggccgtgc cgccgctcgg tggcctcgtc accgaggacg agccctggca
      301 cgtgggcggg cccgtgcaac cggattccgt ggtcgtggtg gccgacttcg tggatccgag
      361 ccgcgccgag atcctggcga tgggctcgat cgggttcctg ccgccggagg ccgacgagga
      421 catcgtcacg gcgatccgcc gggcgcgggt gttcgccggc tactcgggct ggggcgcggg
      481 gcagctggag gccgaactcg ccgaggactc ctggttggtc gagcccgcga tccccgacga
      541 cgtgttccat ccggagcccg gccgcctgtg ggccgatgtg ctccgccgca agggccgaga
      601 cttcgacatg ctgcgcctga tgcccgacga cccagcgatg aactgaccgt tcgctggttg
      661 cggcgaccgg gctctgcgag ggatgctggc tgcatggatc gcacgcccgc gccactcgat
      721 cccgaggccg tcgaacgctg tacgcggccg ttcggcacct cgttcgccat gccgggggcg
      781 gcgcacacct caccggaggt gttcgcctgg gagcaggagc acttcttcgc gccctcgtgg
      841 gtctgcctgg gccgcgccgg cacgcaccgg caggtcgccg ggcaggtcga acgcgccggt
      901 tgggcgttcg ctcgtcgcga cgcggacggc atcgccttcg aggaccagtt cggcaacctc
      961 tgggaccacc tggcgccatg gacacccgag cggttggtcg tcggggcgcg acacagctac
     1021 gagatcgccg cgaactggaa gctgatccac gagaactacc aggagtgcta ccactgctcg
     1081 gagatccacc cggcgctgtg caaggtgacg ccgcccgaca gcgggtactc gatcggcctc
     1141 ctcggcctgt tcgtcgccgg gcctatggac ctgcgtgacg acgtcgagac gatgtcgttg
     1201 gacggtcgct ccggcggcgt cccgatccct ggactcacgg gcaaccaatt gcgcgaggtg
     1261 ggctatttcg gtgtcttccc gaacctgttg atcagccccc accccgacta cgtgctgacc
     1321 catcggatcg aggccctggc gccggatcgg agcttcatcg agtgcgagtg gctgttccca
     1381 cccgaagcct gggaccggcc cggcttcgac ccggcgtacg ccgtggactt ctgggacatc
     1441 acgaaccgtg aggactgggt cgcgtgcgaa tcgctgcagc gctcggccgc ctctcgcggc
     1501 taccggcccg gcccgctctc caccgcgtgg gaggccgggc cctacctgca gacccgcatg
     1561 atcgcgctcg gctaccagga aggcgccgtg actcgatggc cggagcggcc gaagcaggcg
     1621 gaacgtcccc ggcacgaact cctgcgcggc gcatgagcgg agcgtctgtc tcgttcgacc
     1681 gggccgcggc gtactacgac gagacccgcg tcacggaccc ggacgcgctg cgaacgatcg
     1741 tcgacctgct ggcatcggtg atgcccggtc gcgggcccgt gatcgagctc ggcgtcggca
     1801 cgggtcagat cgcgcttccg ttggcggacc gcggcgtgga tgtcaccggc ctcgaccttt
     1861 cggccgagat gatgacggtg ctccgagcca agcccggtgg tcccgccctg ccgctgatcc
     1921 aaggggacgc cacccggctg ccgatcgccg acgggtccct gggtggcgcc tacatgcgat
     1981 gggtgttgca cctgatcccg gattggatgg gggtcttgcg cgagctcgat cgcacggcgg
     2041 tgaccgacgg agccgtcgcg atcgagccgg gcggtttctc cgggccgttc cgggaaacct
     2101 acctgcgata cagagagatc ctgggcgacg cggtcgtcgc tctcggtctc acggcggtcg
     2161 accgagacga gcagctcgac gaaggcttcg cgctcgtcgg atgggcgctt caggacgaag
     2221 ttccggtgat gtacgaacgg accacgacgc tgcaggaggt cttcgacgag gttccctcga
     2281 agcgctggtc gtggacgtgg cgggttcccg acgatgaact cgcgcgcgcg acggacgagg
     2341 tgcgcgcgtg ggccaccgag cacttcggtg acctcgaccg gccgatcccc tcggagcacg
     2401 tccgctggcg tgtgtaccgg agggcggcat gaccgactcg atcgcgttcg accgcgccgc
     2461 cgagtactac gacgccacgc gagggctgtc ggaggagggc gttcgtctca ccactgaggc
     2521 ccttgccgag gtgttcctcg gcgcaggtcc catcctggag gtcggcgtgg gcacgggcca
     2581 ggtggcgctt ccgctgcacg atgcgggcgt ccgcgtgatg ggcgtcgacc tctcccggcc
     2641 catgttggcg aagctccgcg ccaagaccgg tggtgcacgg ccgttcccgt tggtggaggg
     2701 agacgcgacc agcttgtcct tcgccgacga cgcgttcgct ggcgcctacc tgcggtgggt
     2761 gctgcacctg atcccggact ggcgagccgc cgtgcgtgag atcgcgcgca tcctctcgcc
     2821 gggtagcgcg ttcgtggcgg ccctcggcgc ctacggtggt taccggcagg agatgcaggc
     2881 acggttcgcc gagatcaccg gcatctcgac cgaacccgcc ggtctcgcat ggaacggctg
     2941 ggacgaactg gacgctgagc tcgcttcgct ggggctgcag aagctccccg acatcgggtt
     3001 caccgatcaa gaccgggacg acctcgagca tttcatgcag gcgatcgagg gcaaccgatt
     3061 ctcgtggacg tgggccgtac cggacgcagc gacccgagag gccgcggccg ccgaggtgcg
     3121 ccactgggcc gaggaacgct gggggccgct gagcgagatc ccacccgaga ccttcgattg
     3181 gcgcttcgcc gtctaccggc tgccttgacc tgacctactc gtcgacgtag tcgtcggggt
     3241 ggtccacggc gccggggaga tcgtcgacga cgtgcatcgc gtccacctcg ggggcgtccg
     3301 gctcgaacgt ctcggccgat tcgccgacca gggcggcttc gtggtcgagg gcctccacgt
     3361 cggagtcgtc ggtcacgtcg aggtgcttgt gacgccggtc gttctgcggc tgctcccgcc
     3421 gggtgcgatc gtcgatcgtg tcgccggcca tctgctcgga cgtcgtggtg tggtcgtcga
     3481 cgcccaggct gtgatccatc tcagagatgt aggactcgag cgactcaccg ctgtcggcgt
     3541 cgtcgttcga cggtgaggga ccttcggacg gggagtactg gtcgtcggga tcgtgagggt
     3601 cgcgcatccc cggctggtcg ctcgtcatgg gttgagccta cccggggtgg cggcggtgcg
     3661 tgggctcgcc gagcctgggg tcgccccgct atcctggagg gccacgaacg ggcgacccga
     3721 tgagcgcccg ccgacaccag gaggttcgac ggtgccgatc accgaggtcg agaagatctg
     3781 gatggacggc gagttggtgg actggcgcga cgcgcaggtc cacgtgctca gccacgcgct
     3841 gcactacggc agcggcgtct tcgaaggcat ccgggcttac gagaccgtac gcggccccgc
     3901 cgtctggcac ctggacgagc acctcaagcg tctgtaccgc agcgcgaagc tgtaccacct
     3961 ggacatccct ttctcgcctg aggcgttgaa ggacgccacg aaggaggtca tccgcgcgaa
     4021 cgacctccag tcgtgctaca tccggccgct cgcgttccgt gggtacggcg agatcggcgt
     4081 gaacccgttg gagtctcccg tcaacgtcac gatcgcggtg tggccgtggg gcgcctatct
     4141 cggcgaggat gcgctggagc agggggtccg ggtgaaggtg agcagctggc gccgcaacgc
     4201 gcagaactcg ctgcccgccg cggcgaaggc caccggccag tacatcaaca gcgtgctcgc
     4261 caaggtcgag agcctgaagg ccggctacga cgaggcgatc atgctgaacg accacggtta
     4321 cgtcaccgac gggtccggcg agaacatctt catcgttcgc aagggcgtgc tgttcacgcc
     4381 ccccacccag gcgggttcgc tcgacggcat cacgcgggaa accacgatgg ccctcgcgcg
     4441 cgatgccggc tacgaggtcc gcgaagagaa cctggtccgc accgatctct acaacgccga
     4501 ggagtgcttc ttcaccggga ccgcggccga aatcacgccg atccgcgagg tcgatgaccg
     4561 cgaggtcggt gagggccacc gcggcccgat cacgaaggag ctccagtccg cgttcttcgc
     4621 cgccaccaag ggcgagaacc cgaagtatcc ggactggctc acctacgtga acgactgacc
     4681 gcgttcaacc gtcgaaggcg agcgatccca ccaccttcca ctcgccggcg gtggggatcg
     4741 aacccgcttc ccgagcgatg cccaccatga tcggggtgcc gaaggcgtcg gggtcggtgg
     4801 gaacccagaa cgtgtaggtc acgtcgggtc gcagcgagat cgtgcccacg cccgtgatgc
     4861 cgaacagggc gctcgagggt ccggtgttca cgaaggaggc cagcgtcccc agccggccgg
     4921 cgatgatcgg ctcggacacc tcgatggctc cgcgcggcac ctggcccaag atccatgcca
     4981 gcggccggcc gggttgccag cggccggcga cgcccgcgat ggcggatcgc aaccggggtc
     5041 ggacctcgcg ttcgaactcg gcgtcgaaac cgtcgtacca ctgtcgaagc cgcgctggtg
     5101 tctgcgcgta gacgtgcccc accgggtcga ccgggttcag gtgcgcgagg tcgaacaccg
     5161 ccacgctcac cgacccgccg gcgggaccgg tcgtcaccgc agcgaacccg cggccgtccg
     5221 aaccccagat cgaatcgccg gcccgccatc cttcggggag cgtcaccgag gccgcggccg
     5281 agatgtcggg gaagcggtag gtgccggcgt cgagcgcacc ttgcacggga gtcggcgatg
     5341 cgtcagccgg cgctccggag gtcgatcctc gagttccgag gaagatcacc agggccgcca
     5401 gcgacagcgc gcacaccgcc gtggccatcc ggcgacggtc ccgcgttctc cgtcgaagcg
     5461 actggtggcg ggcgaacgtg ccgctggcgg gggcggcggc gagccgcacg gtgtcgagca
     5521 ccgcggcagg ctctgtcggt tcctcgtcgt cggcgaccgt gtcggacagg tctgtggttg
     5581 cagccgcgac ctcctcgcgg atgcgacggg cgcgcatccg caacgcgcga cccgcctcgc
     5641 ccgccgtgag gtgcagggat ccggtgaggg tgatcgcggc gcgacgtcgc cacggcatcg
     5701 tctcgagcac ctgccggacg cggtcgtcga tggtgacatc ctcgaagggc tccagcctgc
     5761 gctcgccgtg caggcgcccc cgggccgaga ccacggctcg ccgccagggc atcctggagg
     5821 ggtcgagtgc cacccggatc gggaatgctg aggggcgttc catctcgcgc gcccgcgccc
     5881 accggtccca cgtgcggagg agggccacat gggctaggtg gtcggcatct cgccgcgacc
     5941 cggtcgcgag gtaggcggcg ccgaacaccc gctcgaactc cagctcgaag aacagttcga
     6001 actcggtccc cggatcgacc tcgggcatgg agaggcgagg atcggcgatg tcatcgcggt
     6061 cgtgagccat gcggcatccc tcgcaatgct ctcggacgtc ccaccatctg accacgatga
     6121 ccggatcctc gcccgaggtt ccttcaagac gcatcgctgg ttctcgcggt aggctcccgc
     6181 tccgatgcag gaccgctacc gtctcccgcg aagcgtgatc ccctcgcgct acgacctcac
     6241 gatcgaaccc gatctggagg ccgccacctt ctcgggaacc gaagccgtga ccgtcgaggt
     6301 ccacgagcgc atcgacgaga tcgtcatcaa cgccctggcc ctcgagatcg acaacgggtg
     6361 gctctccaac gccaccggaa cccggatcga agtgagcggc atcgccttcg acgaggagct
     6421 gcagcgcgcc acgctcggcc ttgccgatcc cgccgatcca ggctcctgga cgctgtacct
     6481 ggcgttccgc ggcgagctca acgatcaact ccacggcttc taccgttcca cctacgcaga
     6541 cgacgacggc gacacccaca cgatcgccac cacgcagttc gaggcggccg atgcccggcg
     6601 cgccttccca tgctgggacg aacccgacct caaggccgtg ttcgccatca ccctgatcgt
     6661 ccccgacggt ctcgccgcca tctccaacgg tcccgaggtg gagcgcgagg atctgggcaa
     6721 tgaccggatc cgtgtccgat tcgccgagac catgagcatg tccacctacc tggtggcgtt
     6781 cgtggtgggc cggctcgagt gcagcgaccc cgtcgatgcc gacggcgtgc cgatccgggt
     6841 cgtctacgtt cctggcaagg gccatctgac gaagttcgcg ctggatgccg ccgtcttctc
     6901 catgtggttc ttcaccgact actacggtct gccgtatccc gatcgcaagg tcgacatgat
     6961 cgcgttgccc gacttcgcgc agggcgcgat ggagaacacc ggctgcatca cctaccgcga
     7021 gtcgctgctg ttggtcgacc cggagcaggc gacgcaaccc gaactcgaga acatcgccga
     7081 cgtcgtcgcc cacgagctgg cccaccagtg gttcggcaac ctcgtgacga tgcggtggtg
     7141 gaacggcatc tggctgaacg aggcgttcgc caccttcatg gcgctggtga cggtcgatgc
     7201 gtggcgaccc gactgggagc gctggtcctc gttcgcccgc gcgtgcacct ccgcgaagga
     7261 ggtcgacgcg ttgcacgcca cgcgcgcgat cgagtacccc gtccattcgc ccgatgacac
     7321 ggccggcatg ttcgacgtgc tcacctacca gaagggcgcg gccaccctgc gtctgctcga
     7381 ccgttacctc gggggcgacg agttccgtga cggcatccgc ctgtacctgc agcgtcacgc
     7441 ctacggcaac accgagaccc acgacctgtg gaacgcgctc gaggaggcca gcggcgaacc
     7501 cgtccgccgg atcatggacc agtggatctg gcagggcggc tacccgctcc tgaccgtctc
     7561 gcccgacgat gaaggcgtcc ggatcgccca gcgtcggttc ctggccgacg gagccgatga
     7621 cgtcaccacc tgggacgtgc cgctgcgggt gcgcgagctc ggcggcgagg agcgctccgt
     7681 gctggttccc gcggggggcg tcagctcgcc ggcagatcct gacgcggcca tggtggcgaa
     7741 cgcgggcgcg agcagcttcg tccgtgtgcg ctacgacgga gatctcctgg accgcctcac
     7801 cgggcgcttg tccgaggtct cgcccctcga acggtacggg ctggtcgacg accactgggc
     7861 cgccgtgagt tccggagccg ccgatgcatc ggcgttcgtc cggttcgcgg cggggttcca
     7921 ggacgaagac gatctggccg tgtggcaggc gttgatggcc ggcctcggct ggtgcgaccg
     7981 cttcgtggaa ggcgaggccc gtgagcggtt ccgcgggttc gttcgtggct tggtggcccc
     8041 cgccatggat cgcctcggct gggaggctcg ggaagaggac gccgatctca ctcgcgcgtt
     8101 gcgcggagcg ctcctgcagg gtctcggtgt gctgggcgcc gatcccaacg ctgaagccgc
     8161 ggcgcgtgaa ttcgaaggcg aggcccgtgc cggcaagccc gtcgatcccg cgctggcggc
     8221 ggccgcggtc aacgtcgtgg cggtggccgg cgataccgat gactacgagc gcttctggga
     8281 ggcctatcgc gatgccccca cgccgcagga gcagctgcgc tacctgatcg cgctgccgct
     8341 cttccgcgac ccggcgctga tggatcgcac gctcgccgcg acgctcggcg acgacatccg
     8401 ctcccagaac gcccccttcg tggtcgcgtt cgccggcacg aaccgcgacc tgggaccgcg
     8461 ctcctggacc ttcctcacag aacactggga cgatctcgtc tcgcgcttcc cgtcctcact
     8521 cacgatccgc atggtcgacg gcgtgcggtt cctctcccgg cccgaggacg tggccgcggc
     8581 cgctgccttc ttcgaacgcc acccgatccc gcagtcggcc aagaccctgc aacagatgct
     8641 ggagcgccag cggatagcag ccgcccttcg cctgcgcgcc acgccggacc tgacggcgca
     8701 cttctcgtag ccgtcgggca tcagccgatc cggatcgctc cacgcatccc gtaggcgtag
     8761 tggccaggga gatggcaccc gaagatcgtc gaaccatggc cggtcgggaa ggtgtacgtc
     8821 gtgaccctcg tcgtcagggc cgggacggac atctctccgg gcttcggcgc gtgatgtgcc
     8881 tcggtcccga gttcatggac cgcttggacc tgctcgttcc ccacgaggaa ttcgtggtcg
     8941 atcggatcgg tgttctccac cacgaagcgc acggtttcgc cgggggcgac gtcgagctcg
     9001 tccggatcga acgctgaatg gtggatcgtg atggggacgg tgcggaggtc ctcggcggcg
     9061 cgggccgagc cgcacgccgg gaccgccacg gccgatgcga gcaggagcgc catgctcacc
     9121 acgagtcgat gaggctgcct catgcggacg atcccgtcct ccgacgtttc gcgatccatc
     9181 cggccgagaa cgccgtcccc acgagcaggg cgatcaccac gacggcc
//
LOCUS       DATDBZ010000428         2181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1581256, whole genome shotgun sequence.
ACCESSION   DATDBZ010000428 DATDBZ010000000
VERSION     DATDBZ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581256"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VIX62_10255"
     CDS             complement(<1..392)
                     /locus_tag="VIX62_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5860656.1"
                     /translation="MGFSREAAAQLTAVDADITAEEQEAIAALPSGSALLIVRRGPNS
                     GARFLLDTDVTTVGRHPDADIFLDDVTVSRRHAEFLRHRMAFEVKDLSSLNGTYFDGV
                     RIETALLSDGAEVQIGKYRLTFYASRRDL"
     gene            complement(503..1123)
                     /locus_tag="VIX62_10260"
     CDS             complement(503..1123)
                     /locus_tag="VIX62_10260"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY5860657.1"
                     /translation="MGDGAGAVGTRIWTIPNVLSMLRLALVPPFLVLIVLGDYVAALI
                     LLIVASLTDLLDGYLARRLGQVTRLGQLLDPAADRLYIFAALVGLAANQLVPWWIVVV
                     IVARDVFLLVLGVVLANHGYGPLPVHQLGKVATFALFFGLPVIMLGLAFPAVEPVSEP
                     VGWAITVWGAFLYWWAGIIYAIETGRVIRIPRVADEPRSDTLEQGG"
     gene            1156..1983
                     /locus_tag="VIX62_10265"
     CDS             1156..1983
                     /locus_tag="VIX62_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY5860658.1"
                     /translation="MGPLVVCLVICAAVIAATWIASLLTREYSWVDRIWSIVPVAYVW
                     VFAGWSGFDARLVLMAVLVTLWGIRLTFNFARKGGYRPGGEDYRWAVLRGRMAPWQYQ
                     LFNLFFISIYQNVLILLFCLPAFTVYEAGPSPLGAWDVVLAIAFMGFLVGEAAADQQQ
                     WVFHRWKTAERAAGRTPEPGFLQTGLFRVSRHPNFFFEQAQWWVFYGFAVAATGIWLH
                     WTVAGAVLLSLLFVGSTVFTESISRSKYPDYDAYRARTSAIVPWFPRGHGAAGERTA"
BASE COUNT          313 a          741 c          785 g          342 t
ORIGIN      
        1 aggtcgcgtc gggaggcgta gaaggtgagc cggtacttgc cgatctgcac ctcggcgccg
       61 tcgctgagga gcgcggtctc gatgcgcacc ccgtcgaagt acgtgccgtt cagcgaggag
      121 aggtccttca cctcgaaggc catgcggtgg cggaggaact ccgcgtgccg gcgcgacacc
      181 gtgacgtcgt cgaggaagat gtcggcgtcg ggatgacgcc ccaccgtcgt cacatcggtg
      241 tcgaggagga agcgcgcgcc gctgttcgga ccgcgtcgca cgatcagcag cgccgagccg
      301 gacggcaggg cggcgatcgc ctcctgctcc tctgcggtga tgtcggcgtc gaccgcggtc
      361 aactgcgctg cggcctcacg gctgaagccg atcgtggtgt cggccgtgcc gtcgcccacg
      421 atgttgttcg cactcgtctc cggagacgtg ccgtcggcct cgcccgctcc gccagcctga
      481 gaaatgtcgg tatccgccac ggctaacctc cctgctccag cgtatccgat cggggctcgt
      541 ccgccacacg gggaatgcgg atgacgcggc ccgtctcgat cgcgtagatg atgcccgccc
      601 accagtacag gaaggcgccc cagacggtga tcgcccaccc caccggttcg ctcaccggct
      661 cgaccgccgg gaaggcgagg ccgagcatga tgacgggcag cccgaagaag agggcgaagg
      721 tcgcgacctt gccgagctgg tgcaccggca ggggcccgta gccgtggttc gcgagcacca
      781 cgccgagcac gaggaggaac acgtcgcggg cgacgatcac caccacgatc caccacggca
      841 cgagctggtt ggcggcgagc ccgacgaggg cggcgaagat gtacaggcgg tcggcggcgg
      901 gatccaggag ctggccgagg cgggtcacct ggccgagccg ccgcgccagg tagccgtcga
      961 gcaggtcggt gaggctcgcg acgatgagga ggatgagcgc cgcgacgtag tcgccgagca
     1021 cgatgagcac gaggaacggc ggcaccaggg cgagccggag catgctgagc acgttcggga
     1081 tcgtccagat cctcgtgccc accgcccccg cgccgtcacc cacgctgcga tcctaccgag
     1141 ccatagaatg cggccatggg tcccctcgtc gtgtgtctcg tcatctgcgc ggccgtgatc
     1201 gccgccacct ggatcgcgtc cctgctgacg cgcgagtact catgggtgga ccgcatctgg
     1261 tcgatcgtgc cggtcgccta cgtctgggtg ttcgccggat ggagcggatt cgacgcccgg
     1321 ctggtgctca tggcggtgct ggtgaccctg tggggcatcc ggctcacctt caacttcgcc
     1381 aggaagggcg ggtaccggcc cggcggcgag gactaccgct gggcggtgct gcgcggccgg
     1441 atggcgccgt ggcagtacca gctgttcaac ctgttcttca tctcgatcta ccagaacgtg
     1501 ctgatcctgc tgttctgcct gccggcgttc accgtgtacg aggccggtcc gtcgccgctg
     1561 ggcgcctggg acgtcgtgct cgcgatcgcc ttcatgggat tcctcgtggg cgaggcggcg
     1621 gccgaccagc agcagtgggt gttccaccgc tggaagacgg ccgagcgcgc tgccgggcgc
     1681 acgcccgagc cggggttcct gcagacgggc ctgttccgcg tctcgcggca tccgaacttc
     1741 ttcttcgagc aggcgcagtg gtgggtgttc tacgggttcg cggtggccgc gacggggatc
     1801 tggctgcact ggaccgtcgc cggcgccgtg ctgctctcgc tgctgttcgt cggctcgacc
     1861 gtgttcaccg agtcgatctc gcgttcgaag taccccgact acgacgccta ccgggcgcgg
     1921 acgtcggcga tcgtgccctg gttcccgcgc gggcacgggg ccgccgggga gcgtacggcc
     1981 tgaccacggt ccggccgacc tgcgaccggc tgcgacctgc gccgcgggcg gcacgcgctc
     2041 acagccggtc gcgcaggtcg ggccacgact cggcgaatcg cggatgcagc ggcagcgcgt
     2101 cgaccccgtc gacggccacc cagcgcagct cgatgctctc ggggtcgctg accaccgggg
     2161 cgaaccgttc ggatgcgtcg g
//
LOCUS       DATDBZ010000429         4967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_767600, whole genome shotgun sequence.
ACCESSION   DATDBZ010000429 DATDBZ010000000
VERSION     DATDBZ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767600"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1057)
                     /locus_tag="VIX62_10270"
     CDS             complement(71..1057)
                     /locus_tag="VIX62_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5860659.1"
                     /translation="MARIRSTAALVVSLVLTAAACTGGGSDTPTTGAPTTSAASTLAA
                     EIVPDDFSVNRPERVQVGIFSSTQDTGVQVVTFGDISVDFTYRGPDGSSAAQPGPSTT
                     FSYLPAPTTAAGGQGPALTNPSDARGVYQGEDIRFDQVGVWTATIEADIDTVGPVTLE
                     SNFAVQEQPALPAAGQKALKTENLTLDSNGVKPVSIDSRAQGGEPIPDPELHEWTIAD
                     AIAQGRPVLVLFATPVYCMSQFCGPTVEALQQLAAEYPDKAVFIHVEIWRDYDKSVIN
                     QGAADWLYRNDNLTEPWLYLIDDKGVIADRWGPLFDTDEVGQVLSQLPDMKG"
     gene            complement(1108..1620)
                     /locus_tag="VIX62_10275"
     CDS             complement(1108..1620)
                     /locus_tag="VIX62_10275"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY5860660.1"
                     /translation="MSELELLVGEVATPLGALTLVASPRGVVATAFDDEDPDTTVAGI
                     EAKLDARARSAQRRLSTVRREVEDYFEGRIRTFATAPDLALAPQGFGRRVLEVTTTIP
                     YGELWTYGDVAEMAGSPRGGRAAGTALSRCPMELFVPCHRVVHAGGTLGGYGRFTERK
                     RWLLRHEGAV"
     gene            complement(1617..2366)
                     /locus_tag="VIX62_10280"
     CDS             complement(1617..2366)
                     /locus_tag="VIX62_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEY5860661.1"
                     /translation="MSRDERALEAGLSDPAVPPALARGELEILGLLPRSSNYTFLVRA
                     TGEDGDQALGVYKPQRGEMPLWDFPEGTLCRREVAAYVVARALGWPNVPPTILRDGPE
                     GLGSVQTFVTFDPEEHYFTLQERFVEDFRAVAAFDLVVNNADRKGGHCLLGEDGRIWL
                     IDHGVCFSAEPKLRTVIWEYMDEPIPPSLLAGIERLGIALDEGGPERHELGALLNEEE
                     LVALRRRIAEIAGSLVFPGPGGDRPFPWPPV"
     gene            complement(2330..2851)
                     /locus_tag="VIX62_10285"
     CDS             complement(2330..2851)
                     /locus_tag="VIX62_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEY5860662.1"
                     /translation="MELGPVDRITADAIGEPGDRAFYLQARAGTEMVTVAVEKEQVQL
                     LSASILELLADLELETGQGPGEEAMALEEPFEPRWRAGKLAIGFDQEQNLFQLEIEEF
                     QPDVEDDDPRSLLLDEPETVRLFATREQMFALSRHGAEVAQRGRPTCQYCGNPMDPEG
                     HACPAMNGHSKRD"
     gene            complement(2865..3533)
                     /locus_tag="VIX62_10290"
     CDS             complement(2865..3533)
                     /locus_tag="VIX62_10290"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5860663.1"
                     /translation="MTTLFLIRHGLTAQTGANLYGRTRGIPLDERGRRQAIHLAERFR
                     TVKLTAIYSSPLERCVQTVEPLAMAQRLPIQERDGLMEMDVGAWTGRTLKSLRRTKRW
                     GEIQGSPSTFRFPEGEGFAEALERIEGEIRSIAKRHPRGRVAVATHGDIVRMLVAHFT
                     GAPLDAFQRTVIDTAGVSVVSLHKGSPHVHLVNDTGGLDRFGPAGTPPPWEVVADPNE
                     PPGT"
     gene            complement(3583..4767)
                     /locus_tag="VIX62_10295"
     CDS             complement(3583..4767)
                     /locus_tag="VIX62_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5860664.1"
                     /translation="MGFEDVAVLIAGVATGVLAGAFFTKRTLSSRVRGLESDLRRSSE
                     ALSRQEHTAREEHQVQDLILDSMQEGVLLFDGAGNTAFANAALEQHLGGRPDGISQLY
                     PPGLREAVRGAGTERGVVTVEFERGEPTRWLRATAAPAGGEGSVLLVVSDVTETKRVE
                     AVRRDFVANASHELKTPAASIQAAAETLVSVAARDPAAVPRFASQLEREALRLSRIVA
                     DLLDLSRLESGSELGQPVRLDALVREEAERFEERCRETDLSLEIDAPSPASVSGSARD
                     LSLLVRNLVDNAIRYTRPGGRITVTVVPGGAVAELRVADDGLGIPTRDLPRVFERFYR
                     VDRARSRETGGTGLGLAIVKHVAENHGGSVEVTSELGRGSTFTVRLPSIGTAAGDTRA
                     VG"
BASE COUNT          736 a         1714 c         1725 g          792 t
ORIGIN      
        1 ggtcggggtg gcggtagcac cgatcgtcgg tgggcggtgg tggtggcgga acggcttgcg
       61 ggtccacggc tcagcccttc atgtcgggca gttgcgacag gacctgtccc acctcgtcgg
      121 tgtcgaacag cggtccccac cggtcggcga tcacgccctt gtcatcgatc aggtacagcc
      181 agggctcggt gaggttgtcg ttgcgataca gccaatccgc cgcgccctgg ttgatcacgc
      241 tcttgtcgta gtcgcgccag atctcgacgt ggatgaacac ggccttgtcc ggatattcgg
      301 cggcgagctg ctgcagcgct tcgaccgtcg gcccacagaa ctgactcatg cagtacaccg
      361 gggtggcgaa caggacgagc accggacgcc cctgggcgat cgcgtccgcg atcgtccact
      421 cgtggagttc gggatcgggg atcggctcgc cgccctgggc tcgcgagtcg atcgagaccg
      481 gcttcacccc gttggagtcc agcgtgaggt tttcggtctt cagcgccttc tgccccgcgg
      541 caggcaacgc cggctgctcc tgcacggcga agttcgactc cagcgtcacc ggcccaaccg
      601 tgtcgatgtc cgcctcgatc gtggcggtcc acacgccgac ctgatcgaac cggatgtcct
      661 cgccttgata cacgcctcgt gcatcgctcg ggttcgtcaa tgccgggcct tggccacctg
      721 cggcggtcgt cggtgccggg aggtaggaga aggtggtgga cggccccggc tgggccgcgg
      781 aggatccgtc gggtccccgg taggtgaagt ccacggagat gtcgccgaac gtgaccacct
      841 ggaccccggt gtcctgcgtg ctcgagaaga tccccacctg cacgcgctcc gggcggttga
      901 cggagaagtc gtcggggacg atctccgcgg cgagggtgga ggccgcgctc gtggtcggcg
      961 caccggtcgt gggcgtgtcg gacccaccgc cggtgcaggc ggcagccgtc aggacgagcg
     1021 acacgacgag cgcggcggtg gatcggatgc gtgccatcgc cctcatcctc tcgcacgatg
     1081 ggacgaacga ccggctcgcc ggagcgctca tacggctccc tcgtggcgga gcaaccatcg
     1141 cttgcgttcg gtgaagcgcc cgtagccgcc caacgtgccg ccggcatgga ccacgcgatg
     1201 gcatggcacg aacagctcca tgggacaccg actcagggcc gtgccggcgg cacgaccgcc
     1261 acgaggactc ccggccatct cggcgacgtc gccgtaggtc cacagttcgc cgtaggggat
     1321 cgtcgtggtg acctcgagca cgcgccggcc gaagccctgg ggggcgagcg ccaggtcggg
     1381 agcggtggcg aaggtccgga tccggccctc gaagtagtcc tccacctccc gccgcacggt
     1441 cgacaggcgc cgctgcgccg aacgcgcacg cgcgtcgagc ttggcctcga tacccgcgac
     1501 cgtcgtgtcg gggtcctcgt cgtcgaacgc cgtcgccacg acccctcggg gtgaggccac
     1561 cagcgtgagg gcccccagcg gggtggcgac ctcgccgacc aggagttcga gctcgctcac
     1621 accggtggcc aggggaaggg acgatcgcca cccgggccgg ggaacacgag cgagcccgcg
     1681 atctcggcga tccggcgtcg caacgcgacc agctcctcct cgttcaacag ggcgccgagc
     1741 tcgtggcgtt cgggcccgcc ctcgtcgagc gcgatgccga gtcgctcgat gccggccagc
     1801 agcgagggtg ggatcggctc gtccatgtac tcccagatca ccgtccgcag cttcggctct
     1861 gcgctgaagc agacgccgtg gtcgatgagc cagatccggc cgtcctctcc gagcaggcag
     1921 tgcccccctt tgcggtcggc gttgttgacc acgagatcga aggcggccac cgcacggaag
     1981 tcctcgacga accgctcctg caacgtgaag tagtgctcct cggggtcgaa ggtaacgaac
     2041 gtctgcacgg agcccaggcc ttcgggaccg tcgcgcagga tcgtgggcgg cacgttcggc
     2101 caccccagcg cacgagccac cacgtaggcc gccacctcgc ggcggcacag cgtcccctcc
     2161 gggaagtccc acaggggcat ctcgccccgt tggggtttgt agacgccgag cgcctggtcg
     2221 ccgtcctcgc cggtggcgcg caccaggaac gtgtagttgg acgagcgggg gagcagaccc
     2281 aggatctcga gctcgccccg cgcgagcgcg ggaggcaccg ccgggtcgct cagtcccgct
     2341 tcgagtgccc gttcatcgcg ggacatgcgt gcccttcggg gtccatgggg ttgccgcagt
     2401 actggcaggt gggacggccg cgttgtgcga cctcggcgcc gtggcgggac agcgcgaaca
     2461 tctgctcgcg ggtggcgaac aatcgcacgg tctcgggctc atcgagcagg agcgaccgcg
     2521 ggtcgtcatc ctccacatcg ggctggaact cctcgatctc caactggaac aggttctgct
     2581 cctggtcgaa cccgatcgcg agtttgcccg cgcgccaccg cggttcgaac ggctcctcca
     2641 gcgccatcgc ctcctcaccg ggcccttggc cggtctcgag ttccaggtcg gcgagcagct
     2701 cgaggatcga ggcggacaac agctgcacct gttccttctc gacggcgacg gtgaccattt
     2761 cagtgccggc gcgggcttgg agatagaacg cgcgatcgcc gggctcgccg atcgcgtcgg
     2821 cggtgattcg gtcgaccgga cccagttcca tggcgatgag tctgtcaggt tccgggtggc
     2881 tcgttggggt cggcgacgac ctcccagggc ggtggcgtac cggccggccc gaaccgatcc
     2941 aggccgccgg tgtcgttcac cagatgcacg tgtggcgagc ccttgtgcag gctgaccacg
     3001 gagacgcccg cggtgtcgat gaccgtgcgc tggaaggcat ccagcggggc gcccgtgaag
     3061 tgcgcgacga gcatccgaac gatgtcgccg tgggtggcca ccgccactcg acctcgcggg
     3121 tgccgcttgg cgatcgaccg gatctcgccc tcgatccgct cgagcgcttc ggcgaatccc
     3181 tcgccctcgg ggaagcggaa ggtcgagggc gatccctgga tctcgcccca ccgtttcgtg
     3241 cggcgcaggc tcttcagcgt gcgcccagtc cacgcgccga cgtccatctc catcaggccg
     3301 tcgcgctcct ggatcggcag gcgttgggcc attgctaacg gttcgacggt ttgcacgcag
     3361 cgctccagcg ggctcgagta gatcgcggtg agcttgaccg tgcggaaccg ttcggcgagg
     3421 tggatcgcct ggcgccggcc gcgttcgtcg agcgggatcc cacgggtacg gccgtacagg
     3481 ttcgcgccgg tctgcgccgt gagcccgtgg cggatcagga acagggtggt catcgcggag
     3541 cagcatacgg gtcccgacga agcgaacctg cgtccggagc cgctagccga ccgcgcgcgt
     3601 gtcgccggcc gccgtcccga tcgagggcag gcgtaccgtg aacgtcgagc cccgcccgag
     3661 ctcgctcgtg acctcgacgc tcccgccgtg gttctcggcg acgtgcttca cgatcgccag
     3721 gcccaagccg gttccgccgg tctcccggga acgcgcacga tcgacccgat agaagcgctc
     3781 gaatacgcgg gggaggtccc gcgtcgggat gcccaggcca tcgtcggcca cgcggagctc
     3841 ggcgaccgcg ccgcccggga ccacggtcac cgtgatccgg ccgccgggcc gcgtgtatcg
     3901 gatggcgttg tccacgaggt tgcgaaccag cagggagagg tctcgagccg aaccggagac
     3961 gctggccggc gatggggcat cgatctccaa ggacaggtcg gtttcgcggc accgttcctc
     4021 gaaacgctcg gcctcctcgc gaaccagggc gtcgaggcgg acgggttggc cgagttcgct
     4081 ccccgattcc agtcgcgaga gatcgagcag atcggccacg atgcgggaca ggcgcagcgc
     4141 ttcccgctcc aactgcgagg cgaaccgcgg cacggcggcc ggatcgcgcg ccgccacgga
     4201 gaccagggtc tcggccgccg cctggatgga agccgcgggc gtcttcagct catgggaagc
     4261 gttggcgacg aagtcccgtc gtaccgcttc gacgcgcttg gtctcggtga cgtcgctgac
     4321 cacgagcagg accgatccct cgccgcccgc gggcgccgcc gtggcgcgga gccagcgggt
     4381 cggttcgccg cgttcgaact caacggtcac cacccctcgt tcggtcccgg ctccgcggac
     4441 cgcttcgcgc aggccggggg gatagagctg ggagatgccg tcgggccgcc cgccgagatg
     4501 ttgctcgagg gcggcgttgg cgaaggcggt gttccccgct ccgtcgaaca gcagcactcc
     4561 ttcctgcatg gagtccaaga tcaagtcctg gacctgatgc tcttcccggg cagtgtgctc
     4621 ctgtcgcgaa agggcctcgg atgatcgccg gaggtcggac tccaggccac gcactcgcga
     4681 ggacaaggtc ctcttcgtga agaaggcacc cgcgaggacg ccggtggcca cgccggcgat
     4741 caggaccgcc acgtcttcga accccatagg agcagtgtag gagcgcaaag ggcttgttca
     4801 cctggcgttc gccggacgtt cgacagaccg tcgggtggga gccgacatca tgtctcccat
     4861 cacggcgttc atcgtccgtt ctcctcctcc cgttcgttca cctgtcgttc atcttcgcgc
     4921 aatcgtccgt ccaccccgtc tgcctaggct ccgccgcgta agcatcc
//
LOCUS       DATDBZ010000430         2233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_544630, whole genome shotgun sequence.
ACCESSION   DATDBZ010000430 DATDBZ010000000
VERSION     DATDBZ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544630"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VIX62_10300"
     CDS             <1..239
                     /locus_tag="VIX62_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003245622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5860665.1"
                     /translation="GIAAFTAAVLGGIGSIGGAALGGFLIGVLQAVGPTLFLNGYGVP
                     SENSLRDAFVFLTLVLVLVFRPGGLLGSGDAEKV"
     gene            239..2038
                     /locus_tag="VIX62_10305"
     CDS             239..2038
                     /locus_tag="VIX62_10305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5860666.1"
                     /translation="MRPGLLGTAAKTGAIAGTVLIFLAAVGLIGNFTDLLLIGEQVTF
                     AGLILVIPPVLAGWISARPKIIAGAVDELTRQHALTAGALAGALSGVTFGAFVVFVEA
                     FGVERIREIFLNISPALMDYLTGGRPPLVAALIITVTSIIVGAAGAGYGTLSDPIRKP
                     IGAAVSITLLMAFLQRIIPIAMDEVGVERDWLYSKVTGGLTWLGAIVIAAVAAGSSIL
                     KERKGDAIRAAIVTRRTRGLEGETKLTPLSVGLMLVGLALLLIAPQVLGSVISEVLGT
                     VMVFALLGLGLNIVVGYAGLLDLGYVFFFTLGAYSLALLTGATLNTFIGSSDPAISLN
                     LNFYVAIPIVIAIAAFAGILVGLPVLRLRGDYLALVTLGLGEVIVVLVTSPWLEPLVG
                     GPQGMRDITNAQIGDFGFREPQHFYYLAFAFVVLALFVSWRLAHSRIGRAWTALREDE
                     QVADAMGVSTTSYKLLAFAIGGAIGSVGGALFAVKIGSLTPASFQVLVSIQVLGIVIL
                     GGIGSLPGVIVGSLVLVGLPGLLREFEEYRLLAYGAALVAVMLLRPEGVIPNVRRSLE
                     LKEEDREQDKWAGDVAGDEHVAPSISPLGEGAG"
     gene            2035..>2233
                     /locus_tag="VIX62_10310"
     CDS             2035..>2233
                     /locus_tag="VIX62_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5860667.1"
                     /translation="MSSLLEVAGITKRFGGLNALDDLSFEASDGEIVSIIGPNGAGKS
                     TVFNVITGLYRPDAGDVRFRGD"
BASE COUNT          304 a          705 c          784 g          440 t
ORIGIN      
        1 ccgggatcgc ggcgttcact gctgccgtgc tcggcgggat cggcagcatc ggtggagccg
       61 ccctgggcgg gttcctgatc ggcgtcctcc aggcggtcgg acccaccctg ttcctgaacg
      121 gatacggggt gcccagcgag aactcgctgc gtgacgcgtt cgtgttcctg acactggtcc
      181 tggtcctggt gttccgaccc ggaggtctcc tcggctccgg cgatgcggag aaggtctgat
      241 gcgccccggg ttgctcggca ccgccgcgaa gaccggcgcg atcgcgggca ccgtattgat
      301 cttcctcgcc gcggtcggac tgatcggcaa cttcaccgac ctgctcttga tcggcgagca
      361 ggtcacgttc gcgggcctga tcctggtgat cccgccggtg ctcgcgggct ggatcagcgc
      421 gagaccgaag atcatcgccg gagccgtcga tgagctgacg cgccagcacg cgctgacggc
      481 cggggccctg gcgggtgcgc tctccggcgt cacgttcggt gcgttcgtcg tcttcgtgga
      541 ggccttcggc gtggagcgca tccgcgagat cttcctcaac atctcgccgg ccctcatgga
      601 ttacctcacg gggggccggc cgccgctcgt ggccgccctc atcatcaccg ttacgtcgat
      661 catcgtcgga gcggcgggtg cgggctacgg gacgctgagc gacccgatcc gcaagccgat
      721 cggggcggcg gtctcgatca cgttgctcat ggccttcctc cagcgcatca tcccgatcgc
      781 gatggacgag gtgggggtcg agcgcgactg gctctacagc aaggtcaccg gcggcctcac
      841 ctggctcggt gcgatcgtga tcgccgcggt cgccgcggga agttcgatcc tcaaggaacg
      901 caagggagat gcgatccgcg cggcgatcgt gacacggcgc acgagaggcc tggagggcga
      961 gacgaagctc acgccgctca gcgtggggct gatgctggtc gggctcgcct tgctgctgat
     1021 cgccccgcag gtcctcggct cggtgatctc cgaggtgctc ggcaccgtca tggtcttcgc
     1081 cttgctcggt ctcggtctga acatcgtcgt gggctacgcg ggactgttgg acctggggta
     1141 cgtgttcttc ttcaccctcg gcgcctactc cctcgccctg ctcactgggg cgaccctgaa
     1201 caccttcatc ggatcgagcg atcccgcgat ctcgctcaac ctcaacttct acgtcgcgat
     1261 cccgatcgtg atcgcgatcg ccgcgttcgc cggcatcctc gtggggctcc ccgtcctgcg
     1321 gctgcggggt gactacctcg ccctcgtgac cctggggctc ggcgaggtga tcgtggtcct
     1381 ggtcacgagt ccgtggttgg agccgttggt cggtggccct cagggcatgc gggacatcac
     1441 gaacgcgcag atcggtgact tcgggttccg agaaccacag cacttctact acctcgcatt
     1501 cgcgttcgtg gtgttggcgt tgttcgtgtc gtggcgtctg gcgcattcac gcatcggccg
     1561 ggcgtggacg gccttgcgcg aggacgaaca ggtcgccgat gcgatgggcg tcagcacgac
     1621 gtcgtacaag ctgctcgcat tcgcgatcgg cggcgcgatc ggctcggtag gtggtgcgtt
     1681 gttcgcggtg aagatcggct cgctcacccc ggcgagcttc caagtgttgg tctcgatcca
     1741 ggtgctgggc atcgtgatcc tgggtggcat cggcagtctg ccgggcgtga tcgtcggctc
     1801 gctggtgctg gtcggcctgc ccgggttgtt gcgtgagttc gaggagtacc ggctgctggc
     1861 ctatggggcg gcgctggttg ctgtgatgtt gctgcgaccc gaaggcgtga tcccgaacgt
     1921 tcggcggagc ttggagctga aggaagagga tcgagaacag gacaagtggg cgggtgatgt
     1981 cgcgggcgac gagcacgtcg cgccctcgat ctcgccgctc ggtgagggcg caggatgagc
     2041 tcgctcctcg aggtcgcggg catcaccaag cgtttcgggg gattgaacgc gctcgacgac
     2101 ctgtcgttcg aagcatccga cggtgagatc gtgtcgatca tcggaccgaa cggggccggg
     2161 aaatccaccg tcttcaacgt gatcacgggt ctctatcgcc ccgacgcggg cgacgtgcgg
     2221 ttccggggag act
//
LOCUS       DATDBZ010000431         3042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_768778, whole genome shotgun sequence.
ACCESSION   DATDBZ010000431 DATDBZ010000000
VERSION     DATDBZ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768778"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VIX62_10315"
     CDS             <1..1059
                     /locus_tag="VIX62_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5860668.1"
                     /translation="DAGYGALTWPVEFGGAGLPQGFATAFEELEAGYAHPPHHETFSV
                     TLHLVAPTLAQFGQPELRDQLLPRLVRTDVLSCQLFSEPGAGSDLGALATRAERDGDS
                     WVINGQKTWSSGARFAEWGELVARSDFDVPKHQGMTAFMMRMDSPGVTIRPIRQMSGG
                     SSFNEVFFSDVRVPDSQRLGEVGEGWRVALTTLGFERGSSGSGRGSSTVGGSFEQLLS
                     LARWLDRTGDPVMRQRLADVYAHERLREFDAARTLASAAGDGAPGPSGSIGKLLWTRS
                     MTRIGSVAADLLGPRMIADTGEWGTYAWTKQVLGAPGYRIAGGSDEIQHDIIANRVLG
                     LPAEPRIDKGVAFRNVPR"
     gene            1124..1894
                     /locus_tag="VIX62_10320"
     CDS             1124..1894
                     /locus_tag="VIX62_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY5860669.1"
                     /translation="MPKPAPRMKIEAGTAVLRPLKTSETVARDIVHSVVTGGLGNGDS
                     LPSEARMLEQYGVSRESLREGLRLLEAQGLITLRRGPGGGPVVGKVDPANLGRVSTLY
                     YHLVGANYGELFEAWAVAEGYLAERAARNEDRALVRATLEPYLTVEDPDLGDLEEFVA
                     HHTEFHTDVGALAGNRVMQLSLMAIGQTVTHHIANTADPRDVADILLHDHRLIAAAII
                     AGKPIKARDLMRVHLEGVADFYDQLSGGEILDQLIEWR"
     gene            2008..>3042
                     /locus_tag="VIX62_10325"
     CDS             2008..>3042
                     /locus_tag="VIX62_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5860670.1"
                     /translation="MSWDFETDPEFQAELDWVDDFVRNEVEPVDQVVHHAWDMKDPVR
                     KALIPPLQQQVRDRGLWACHLGPSLGGPGYGQVKLALLNEILGRSHSAPIVFGCQAPD
                     SGNAEILAHYGTDELKKRYLAPLLAQEVVSAYSMTEPRGGSDPTGFVTRAERDGDEWV
                     INGEKWFSSHANFAEFLIVLAVTDPEEERHRRMSMFVVDIDTPGVEIVRNVGIYGHLE
                     EEATHSYVRYTDVRIPADHLLGERGEGFQVAQTRLGGGRIHHAMRTIGLVKSAFDQMC
                     ERALSRSTQGETLARKQLVQAMIADSWIQIEQFRLLVLRTAWKIDRYNDYLKVRADIS
                     AVKAAMPKVLH"
BASE COUNT          515 a         1021 c         1016 g          490 t
ORIGIN      
        1 gacgccggat acggcgcgct cacgtggccc gtcgagttcg gcggcgccgg gttacctcag
       61 gggttcgcca ccgctttcga agagctggag gcgggctacg cgcacccgcc ccatcacgag
      121 acgttcagcg tgacgctcca cctcgtggcg ccaacgctcg cccagttcgg acagcccgag
      181 ctgcgggatc aactgttgcc gcgtctcgtg cgaaccgacg tgctgagctg ccagctgttc
      241 tccgagccgg gggcgggatc cgacctcggc gcgctcgcga ctcgcgccga gcgcgacggg
      301 gactcctggg tcatcaacgg gcagaagacg tggagctccg gcgcccggtt cgccgagtgg
      361 ggagagctcg tcgcccgcag cgatttcgac gtaccgaagc atcagggcat gacggcgttc
      421 atgatgcgga tggacagccc cggcgtgacg atccggccga tccggcagat gtccggcggc
      481 tcgtccttca acgaggtgtt cttctccgac gttcgcgtcc ccgactcgca gcgcctcggt
      541 gaggtcggcg agggttggcg ggtcgcgctc accacgctcg gattcgaacg tggctccagc
      601 ggttccggac gcggcagcag cactgtcggc ggatcgttcg aacagttgct cagcttggcc
      661 agatggctgg accgtacggg cgacccggtg atgcggcagc ggctcgccga cgtctacgcc
      721 cacgagcggc tccgtgagtt cgacgccgca cgaacgctgg catccgccgc cggcgacggc
      781 gcgcccgggc cgtccggatc gatcggcaag ctgctgtgga cgagatcgat gactcggatc
      841 ggcagcgtcg ccgccgacct gctcggacca cgaatgatcg ccgacaccgg cgagtggggg
      901 acgtacgcct ggacgaagca ggtgctcggt gccccgggct accggatcgc cggcggcagc
      961 gacgagatcc agcacgacat catcgccaac cgggtcctcg gcctaccggc cgaacctcga
     1021 atcgacaaag gcgtagcgtt ccggaacgtt ccacgatgat cccttcggcc cgtcgttggg
     1081 cagggtccgg ggtggacgat gcggcgaccg gtagcctgcg gccgtgccga agccagcgcc
     1141 gcggatgaag atcgaagcgg gcaccgcggt gttgcgtccc ctgaagacgt ctgagaccgt
     1201 cgctcgcgac atcgtccact ccgtcgtcac cggcggcctc ggcaacggtg acagcctccc
     1261 gtccgaagct cgcatgctcg agcagtacgg agtgagccga gagtcgctcc gggaggggct
     1321 gcgcttgctg gaagcgcaag gcctgatcac acttcgccgg ggccccggcg gcggtcccgt
     1381 ggtcggcaag gttgatcccg ccaacctcgg tcgcgtctcc acgctctact accacctcgt
     1441 gggtgccaac tatggcgagc tgttcgaagc ctgggcagtg gcggagggct acctcgccga
     1501 gcgtgccgct cgcaacgagg accgggcgct cgtgcgtgcc accctcgagc cgtacctcac
     1561 cgtcgaggac ccggacctcg gcgatctcga agagttcgtc gcgcaccaca cggagttcca
     1621 caccgacgtc ggagccctgg ccgggaatcg ggtgatgcag ctgtcgctca tggcgatcgg
     1681 gcagacggtc acgcaccaca tcgccaacac cgcggaccct cgcgatgtcg ccgacatcct
     1741 cctccacgat catcgcttga tcgccgccgc gatcatcgct gggaaaccca tcaaggcgcg
     1801 cgacctgatg cgcgtccatc tggagggcgt ggccgacttc tacgatcagt tgtcgggcgg
     1861 ggagatcctc gatcagctga tcgagtggcg ttgaccgagc caggtcgcgc cgtatttatc
     1921 atgatgaact cttgacaagg gcggtatgtt cggctctcat gggagcagcc gcattgtcaa
     1981 gctgcacggt caagagggga tgctgtcgtg agttgggact tcgaaaccga tcctgagttc
     2041 caggccgagc tcgattgggt cgacgacttc gtacgcaacg aggtcgagcc ggtcgatcag
     2101 gtcgttcatc atgcgtggga catgaaggat cccgtgcgta aggccctgat ccccccactc
     2161 cagcagcagg tccgcgaccg cggcttgtgg gcctgccatc tcggccccag cctcggcggg
     2221 cccggctacg gccaggtgaa gctcgccctg ctcaacgaga tcctcggacg gtcgcacagc
     2281 gccccgatcg tgttcggttg ccaggcgccg gactccggca acgccgagat cctcgcccac
     2341 tacggcaccg acgagttgaa gaagcgctac ctcgcgccgc tgctcgcgca ggaggtggtc
     2401 tcggcgtact cgatgaccga gccgcgcggt ggctccgacc cgaccgggtt cgtcacccgt
     2461 gccgagcgcg atggcgacga atgggtgatc aacggagaga agtggttctc gtcccacgcc
     2521 aacttcgccg agttcctcat cgtcctcgcg gtcaccgacc ccgaagagga gcgtcaccgg
     2581 aggatgtcga tgttcgtggt cgacatcgat acgccgggcg tcgagatcgt ccgcaacgtc
     2641 ggcatctacg gccacctcga ggaagaagca acccacagct acgtgcgcta caccgacgtg
     2701 cggatcccgg ccgatcacct gctgggcgag cgcggcgagg gattccaggt ggcacagacg
     2761 cggctcggcg gcgggcgcat ccatcacgcc atgcgcacca tcgggctcgt gaagagcgcg
     2821 ttcgaccaga tgtgcgaacg cgccctctcg cgttccaccc aaggcgagac gctggcgcga
     2881 aagcaactcg tacaggcgat gatcgccgac tcgtggatcc agatcgagca gtttcgcctc
     2941 ctcgtcctgc gcacggcgtg gaagatcgac cgctacaacg actacctcaa ggtcagagcc
     3001 gacatctcgg cggtgaaggc ggcgatgccg aaggtgttgc ac
//
LOCUS       DATDBZ010000432         3402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1785550, whole genome shotgun sequence.
ACCESSION   DATDBZ010000432 DATDBZ010000000
VERSION     DATDBZ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785550"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VIX62_10330"
     CDS             complement(<1..258)
                     /locus_tag="VIX62_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860671.1"
                     /translation="MSIRVVLGEDNVLVREGVRALLDSYDDIEVVGVAEDAPSLLATA
                     AEHEPDVVVTDIKMPPSFQLEGIDCAHSIRDSHPDTGVVVLS"
     gene            303..983
                     /locus_tag="VIX62_10335"
     CDS             303..983
                     /locus_tag="VIX62_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5860672.1"
                     /translation="MTRPTLRVVFAEDNYLVREGTAALLASVDTVDLVGTAETRDELL
                     AAVTELAPDAVLTDIRMPPTNSNEGIEAAKLIRETHPQIGVVVLSQFADEEYAYELLK
                     DGAAGLGYLLKERVADVDELVRALEEVARGGSVLDPKVVESLVAAKDRMAHSPLATLT
                     DREREVLSHMAQGQNNAAIAKSLFLTERAVEKHINSLFHKLDLTEEPDVHRRVMAVLA
                     FLRETDQP"
     gene            complement(986..1393)
                     /locus_tag="VIX62_10340"
     CDS             complement(986..1393)
                     /locus_tag="VIX62_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5860673.1"
                     /translation="MTTILVGTDTTASADLAVEDAAALAHARGAELLVLYVCKDGDLR
                     TVVDPAKSADPSGYLARMGERFPGLATRTRTETGDAAERIVEVAAEEHADTIVVGNRG
                     VHGSWWRVKDSVPNLVLRHAPCSVFIVDTRRAQ"
     gene            1553..>3402
                     /locus_tag="VIX62_10345"
     CDS             1553..>3402
                     /locus_tag="VIX62_10345"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5860674.1"
                     /translation="MEATVTPDSSGGDRPAASRRTARTVAIVLCAGASVAAFALRATA
                     PPDPSPATIVVIGDPTADRVQEVREESEALIADGARFGQSQASTVIGLIAVLLACLGW
                     MAVGALIVSTQPRNWAGWIFLAIGATLPLTNLCQVLATVGARRDPGSIPLLGPIAYVG
                     FYLIFPIAMLPLLFLLYPDGHVPSRGWRWAVRGLLGGTSLAILGYVLSPGPINSLITD
                     GILYVNPTGIDGFGSVSSSLIRLGTIVALISSLSTVVAIRQRYRRSTGTLREQMRLLV
                     LVAATAGATMLLGFVGTVVGALLGREDDPAFFLALFAFLVLTLLVGVPGAYLIAIFRH
                     GLWDMGLVIRKTVQYGVLVVAFMLLGFVIVALVPTLLIGVGGTDYVPTVIIAAFLAGA
                     FLWLRPRATRVANRLVYGRRATPYEVLSEFSERVGETYSTDDVLPRMAHLLVQATGAR
                     RADVWLRVGTELRCDARWPTDVEPPPTRRIRGDEIAGPHDDYLAEVRHQDELLGAITL
                     DLPADDPMNPSKEALVRDLAAQAGLVLRNVRLIEELRASRQRLVAAQDEERRKLERNI
                     HDGVQQQLVALTVQLRLAEQLTERDPAKARELMASLQGRTTGALEDLRDLA"
BASE COUNT          509 a         1177 c         1149 g          567 t
ORIGIN      
        1 gctgaggacg acgacgccgg tgtcggggtg gctgtcacgg atcgaatgcg cacagtcgat
       61 cccttcgagc tggaagctcg gcggcatctt gatgtcggtg accacgacat cgggttcgtg
      121 ttcggccgcg gtggcgagca acgagggggc atcctcggcc acccccacga cctcgatgtc
      181 gtcgtaggag tccaacagcg ctcgcacgcc ctcgcgcacc agcacgttgt cctcgccgag
      241 cacgaccctg atactcacgc cgctcgcctt cctggccggg ggtgcacgtg tagcatccgc
      301 gcatgacccg accaacactc cgggtggtgt tcgcggagga taactacctc gtgcgcgaag
      361 gaacagccgc gttgttggca tcggtcgaca ccgtggacct cgtgggaacg gccgagacgc
      421 gcgacgaact gctcgccgcc gtcaccgaac tggctcccga cgcggtgctg accgacatcc
      481 ggatgccgcc gacgaacagc aacgaaggca tcgaggcggc caagctgatc cgcgagacgc
      541 acccgcagat cggcgtggtc gtgctctccc agttcgcgga cgaggagtat gcctacgagc
      601 tgctgaagga cggcgccgcc ggcctcggct acctgttgaa ggagcgtgtc gccgacgtgg
      661 acgaactcgt tcgcgccctc gaggaggtcg cccgaggagg cagcgtgctc gatcccaagg
      721 tggtcgaatc gctggtggcc gccaaggacc ggatggcgca ttcgccgctg gccacgctca
      781 ccgaccgcga gcgcgaggtc ctctcgcaca tggcgcaggg gcagaacaac gccgcgatcg
      841 cgaagtcgct cttcctcact gagcgtgccg tcgagaaaca catcaactcg ttgttccaca
      901 agctggacct cacggaggaa ccggacgtgc accgccgcgt catggcggtc ctggcgttcc
      961 tccgcgagac cgatcagccc tagcgtcact gcgctctccg cgtgtccacg atgaacacgc
     1021 tgcagggggc atggcgtaac acgaggttcg ggacgctgtc cttgacgcgc caccagctcc
     1081 cgtggacgcc tcggttgccc accacgatcg tgtcggcgtg ctcttcggcc gcgacctcga
     1141 cgatgcgctc ggcggcgtcg cccgtctcgg tccgtgtccg ggtcgccagg ccggggaacc
     1201 gctcccccat gcgggcgaga tagccggacg ggtccgcgga cttggccgga tccaccaccg
     1261 tgcggaggtc gccgtccttg cagacgtaga gcacgaggag ctcggctccc cgcgcgtggg
     1321 ccagggcggc ggcgtcctcg accgcgaggt cggccgaggc cgtggtgtcg gtgcctacca
     1381 ggatggtcgt catcgtgggt catctccttg ggttgtctgc gatctcaccg taggccccct
     1441 gacctgcgag gacgacccgg ccgccaccac ggttggcggg ggtggaggca cctggcgaag
     1501 aggtggcgtt ctccccctgg ctctgtgggg acgcggatgc gaccatcgcg gcatggaggc
     1561 aaccgtgaca cccgactcct cgggtggcga ccggccggcc gcgagcagac gcaccgctcg
     1621 cacggtcgcg atcgtgctgt gcgccggcgc ttcggtggcc gcattcgcgc tccgagcaac
     1681 cgccccaccc gacccgtcgc ccgcaacgat cgtcgtcatc ggcgacccca cggcggatcg
     1741 cgtccaggag gtccgggaag agtctgaggc gttgatcgcc gacggtgcgc gcttcggcca
     1801 gagccaggcg agcacggtga tcggcctgat cgcggtgctc ctcgcgtgcc tgggctggat
     1861 ggcggtcggc gcgctgatcg tgtcgacaca accccggaac tgggcgggat ggatcttcct
     1921 ggcgatcggc gcgaccctgc cactcacgaa cctgtgccag gtgctggcca ccgtcggcgc
     1981 acgccgcgac cccggttcga tcccgctcct cggcccgatc gcctacgtcg gcttctatct
     2041 gatcttcccc atcgccatgc tcccgctgtt gttcctcctc tacccggacg gtcatgtgcc
     2101 gagtcggggt tggcgctggg ccgtccgtgg cctgctgggg gggaccagcc tggcgatcct
     2161 cggatacgtc ctgtcccccg gcccgatcaa ctcgctcatc acagacggga tcctctacgt
     2221 caatcccaca gggatcgatg gattcggctc ggtatccagc tcgctcatca ggttaggcac
     2281 gatcgttgcg ctgatctcct cgctctccac ggtggtcgcg atccgccagc gctatcgccg
     2341 atccacgggg accttgcgag agcagatgcg actgctggtg ctcgtggcag cgaccgcggg
     2401 agcgaccatg ctcctggggt tcgtcggaac cgtcgtcgga gccctcctcg gacgggaaga
     2461 cgacccggca ttcttcttgg cgctgttcgc tttcctggtc ctgacgttgt tggtcggcgt
     2521 gccgggcgcc tacctcatcg cgatcttccg tcacggcctc tgggacatgg gcctggtgat
     2581 ccgcaagacg gtgcagtacg gcgtgctggt cgtcgcgttc atgctcctgg ggttcgtcat
     2641 cgtggcgttg gtcccgaccc tgctgatcgg cgtcggtggc accgactacg tgccgacggt
     2701 catcatcgcg gcgttcctcg ccggggcctt cctgtggctg cgaccgcgcg ccacccgcgt
     2761 cgcgaaccgg ctggtctatg gccggcgcgc caccccgtac gaggtgctct cggagttcag
     2821 cgaacgggtc ggggagacct actccaccga cgacgtgttg ccacggatgg cccatctgct
     2881 cgtgcaggcc accggtgccc gacgggccga cgtgtggctg cgcgtgggca ccgagctgcg
     2941 ctgcgatgcg aggtggccga ccgacgtcga acccccgccg acccgacgca tccgcggcga
     3001 tgagatcgcg ggaccgcacg acgactacct cgccgaggtc cggcaccagg acgaactgct
     3061 gggggcgatc acgctcgacc ttcctgcgga cgacccgatg aacccgtcca aggaggcgct
     3121 cgttcgcgac ctcgcagctc aggcaggact cgtccttcgc aacgtgcggc tgatcgagga
     3181 gctgcgggcc tcacgacagc ggttggtggc ggcccaggac gaggaacgcc ggaagctgga
     3241 gcgcaacatc cacgatggtg tgcagcaaca gctcgtggcg ctgaccgtgc agctccgtct
     3301 ggccgaacag ctcaccgagc gcgacccggc gaaggcacgg gaactcatgg cctcgctcca
     3361 gggccgaacc accggggccc tggaggatct acgggatctg gc
//
LOCUS       DATDBZ010000433         2786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_547036, whole genome shotgun sequence.
ACCESSION   DATDBZ010000433 DATDBZ010000000
VERSION     DATDBZ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547036"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VIX62_10350"
     CDS             complement(<1..969)
                     /locus_tag="VIX62_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY5860675.1"
                     /translation="MSTRSASRRSVLLRLVVLLVALVGLYVVWPSLLGELDRLPELDT
                     LNPIWFVVMFACEAASFACLWMLQRLMLRSTRWYAVATAQLTENAVAKLLPGGTAAGE
                     ALQYRMLTTAGERPEKVGVALAASEVVDVATVLALPVLSLPFILLGSGVAPGLGRSAV
                     VGVGILLLVTAVMIPLVAYDRPLRWVGRTIARARPSTGSDLPERLITQRDQIREVLSA
                     RWWQAIVFAAGNWGFDYLALVTAVAAFDPGFPNPALILLAYVTGQVLSTISITPGGVG
                     FVEAGLAAMLVLAGVPRGSAALATLAYRLVSFWLALPAGGLAYWRWH"
     gene            complement(966..1616)
                     /locus_tag="VIX62_10355"
     CDS             complement(966..1616)
                     /locus_tag="VIX62_10355"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEY5860676.1"
                     /translation="MTQVATRTPTPAPPARWNTRRSGWRLGLHWFGVVCLMVAAGFGG
                     YVVWLLWGTGLSTQRAQDSLRSGFEQAIDTKDPSQAPDRIPIGDAYAQLLIPSIGVNF
                     MVVQGTEYEDLKLGPGHYVDTANPWDDTGRVGIAGHRTTYLHPFYQLDKVVAGDRITL
                     RTEFGTYDYVVTRVFAVNEYGSGRVLEQTAEPTLVLTTCHPKYFSSQRLIVYADRV"
     gene            1719..>2786
                     /locus_tag="VIX62_10360"
     CDS             1719..>2786
                     /locus_tag="VIX62_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5860677.1"
                     /translation="MNAVSPLIQGLNDIQTEAVLHTEGPVLIVAGAGSGKTRALTHRI
                     AYLIREEGVSPGQILAITFTNKAAREMVARVEELLGERVAKGMWILTFHSTCARLLRR
                     EHAFLGVPSGFTIYDDGDTERLINGILKDLDLDPKRFAPRAMASGISRAKDQVLGPDE
                     FAQMASNYYEETVAKVFSAYEARKKAAGALDFDDLISETVRLFRDHPEVLAHYQERFR
                     YLMVDEYQDTNRAQYELVNLLAAKHRNVCVVGDADQGVYSWRGATIQNILDFERDYPD
                     AQVFLMEQNYRSTQNILEAANALIEHNQQRKPKALWTEAGSGELIVRFRADDEHEEAF
                     FVVDEIERLRETEGFRLSDVAV"
BASE COUNT          485 a          992 c          915 g          394 t
ORIGIN      
        1 gtgccagcgc cagtacgcca gcccgccggc cgggagcgcg agccagaagg acaccaaccg
       61 gtaggccaac gtcgccaggg ccgccgaacc tcgcggcacg cccgctagca ccagcatcgc
      121 cgccaggccg gcctcgacga agccgacgcc tccgggcgtg atcgagatcg tcgacagcac
      181 ctggcccgtg acgtaggcga gcaggatcag cgccgggttc gggaacccgg gatcgaacgc
      241 cgccaccgcg gtcaccagcg ccaagtagtc gaacccccag ttccccgccg cgaacacgat
      301 ggcctgccac catctcgccg agagcacctc ccggatctgg tcgcgctggg tgatcagccg
      361 ttccgggagg tcgcttcccg tcgagggccg tgccctcgcg atcgtccggc ccacccaccg
      421 caagggtcgg tcgtaggcca ccaacgggat catcacggcg gtcaccagga gcaggatccc
      481 cacgcccacc acggcgctcc tcccgaggcc cggcgccacc ccgctcccca gcaggatgaa
      541 cggcaggctc agcaccggca gcgccagcac cgtcgccacg tcgaccacct cggacgcagc
      601 cagcgcgacc cccacctttt cggggcgctc cccggcggtg gtcagcatcc ggtactggag
      661 cgcctcgccg gcggcggtgc cgcccggcag cagtttggcc accgcgttct cggtgagttg
      721 tgccgtggcg acggcgtacc accgcgtgct gcgcagcatg agccgctgca gcatccacag
      781 gcacgcgaag ctcgccgcct cgcacgcgaa catcaccacg aaccagatcg ggttgagcgt
      841 gtccagttcg ggcagccggt ccaactcacc caggagcgac ggccacacca cgtagagccc
      901 caccaacgcc accaggagca cgacgaggcg aaggagcacg ctgcgccgcg acgccgacct
      961 cgtgctcaca cgcgatcggc gtacacgatc aatcgctggg agctgaagta cttcgggtga
     1021 caggtggtga ggaccagcgt gggttcggcc gtctgttcca ggaccctgcc cgacccgtac
     1081 tcgttgaccg cgaatacccg ggtcaccacg tagtcgtacg tgccgaactc ggtgcgcagc
     1141 gtgatgcggt caccggccac gaccttgtcc agctggtaga acgggtgcag gtacgtagtg
     1201 cggtggccgg cgatgcccac gcgacccgtg tcgtcccacg ggttcgccgt gtcgacgtag
     1261 tggcccggtc ccagtttcag gtcctcgtac tcggtgccct gcaccaccat gaagttcacg
     1321 ccgatcgagg ggatcaataa ctgcgcgtag gcgtccccga tcgggatccg gtcaggagcc
     1381 tgcgacgggt ccttggtgtc gatcgcctgc tcgaacccgg accgcaggct gtcctgcgcc
     1441 cgctgtgtcg agagtcccgt gccccacagg agccacacca cgtagccgcc gaacccggcc
     1501 gcgaccatga ggcagaccac gccgaaccag tggaggccca gccgccaccc gctccggcgg
     1561 gtgttccacc gagcgggggg tgcgggggtc ggggtgcggg tcgccacctg cgtcatcagg
     1621 cttccacggt accgcccgtt cctcgttggc gcacccaccg gacggcccgt tcggcgaggc
     1681 gaacggctgg ggtcgccggg ggctcctaca ctggggtcgt gaacgccgtc tcgcccttga
     1741 tccaggggct caatgacatc cagaccgagg ccgtcctgca cacggagggg ccggtcctca
     1801 tcgtggccgg ggccggcagc ggcaagaccc gggccctcac ccaccgcatc gcctacctga
     1861 tccgcgaaga gggggtctcg cccggccaga tcctggcgat caccttcacg aacaaggccg
     1921 ctcgcgagat ggtcgcgcgt gtcgaagagt tgctgggcga acgcgtcgcc aagggcatgt
     1981 ggatcctcac gttccactcc acgtgtgctc gcctcctccg tcgtgagcac gcgttcctgg
     2041 gggtgccgag cgggttcacg atctacgacg acggtgacac ggaacggttg atcaacggca
     2101 tcctgaagga cctcgacctc gacccgaaac ggttcgcgcc cagggcaatg gcctcgggca
     2161 tctcgcgcgc gaaggaccaa gtgctggggc ccgacgagtt cgcccagatg gcctcgaact
     2221 actacgagga gaccgtcgcg aaggtgttct ccgcctacga agcgcgcaag aaggcggcgg
     2281 gggcgctcga cttcgacgac ctgatcagcg agaccgttcg gctgttccgc gaccaccccg
     2341 aggtgctggc ccactaccag gagcggttcc gctacctgat ggttgacgag taccaggaca
     2401 cgaaccgcgc gcagtacgag ctcgtgaacc tgctcgcggc gaagcaccgc aacgtgtgcg
     2461 tcgtgggcga cgccgaccag ggtgtgtaca gctggcgcgg cgccacgatc cagaacatct
     2521 tggacttcga gcgcgactac cccgatgcgc aggtgttcct gatggagcag aactaccgct
     2581 ccacgcagaa catcctggaa gcggcgaacg cgctgatcga gcacaaccag cagcgcaagc
     2641 cgaaagccct gtggaccgag gcgggctcgg gcgagctgat cgtgcggttc cgtgccgacg
     2701 acgaacacga ggaggccttc ttcgtggtcg acgagatcga gcgcttgcgc gagaccgaag
     2761 gcttccggct ctccgacgtg gcggtc
//
LOCUS       DATDBZ010000434         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_967552, whole genome shotgun sequence.
ACCESSION   DATDBZ010000434 DATDBZ010000000
VERSION     DATDBZ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967552"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VIX62_10365"
     CDS             <1..631
                     /locus_tag="VIX62_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY5860678.1"
                     /translation="IELPSGSDVRGAIAYGNLRAEGRLAACTVKTGAGDITLDQAGPL
                     DVRTGAGTVSIAVAIGHCDVESASGSVRIRRVEGSAAIKNSYGDTTIGEVTGELRVKG
                     AYGSIWVDRALGSVDVRTAHGNVRLDEIVRGEVQLESSYGALEVGVREGTAVWLDVAT
                     QHGSVINSLGAHDGPGGADETAEVRARSSWGEIVIRRSNERDTANRSNS"
     gene            664..1476
                     /locus_tag="VIX62_10370"
     CDS             664..1476
                     /locus_tag="VIX62_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5860679.1"
                     /translation="MSTTGRNGGPAIRVQGIEKSYNDLHVLRGVDFEVAAGSIFALLG
                     SNGAGKTTIVRMLSTLLKQDAGTASVNGFDVATQPTKVRESISLTGQFAAVDEILTGR
                     ENLVLVARLRHLQDPARIADELLARFDLTDAATRKVSTYSGGMRRRLDISMSLIGQPK
                     VIFLDEPTTGLDPEGRLEVWRVIQDLATRGTTVLLTTQYLDEAEQLADRIAILHQGRI
                     IANGTLAELKKLLPPATVEYVEKQPTLEEVFLTLVGNDDGNHDTAQTRKDQS"
     gene            1473..2234
                     /locus_tag="VIX62_10375"
     CDS             1473..2234
                     /locus_tag="VIX62_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860680.1"
                     /translation="MSTHFFADTALLTGRSLRHILRSPDTIITTAITPIALLLLFVYV
                     FGGAIQTGTDDYVTYLLPGILLIAVASGIAYTAFRLFLDLQSGIFERFHSMPIARSSV
                     LWAHVLTSLVANGLTLVIIVGVALLMGFRTSAGLLAWLAVAGILALFTLALTWLAVIA
                     GLTAKTVDGASGFSYVLILLPFISSAFVPTDTMPGPVRAFAENQPVTSIVNTIQDLFA
                     GQPVGTDIWIALAWCVGILVIAYVIAMAIYRRKIA"
     gene            complement(2275..>2633)
                     /locus_tag="VIX62_10380"
     CDS             complement(2275..>2633)
                     /locus_tag="VIX62_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860681.1"
                     /translation="RLDGGVLDVRILHAGSRARAAASLAFGPRISAVLRGLRLLPRRI
                     ESFTAESIDVVVRPREGQPPGFAHDGEVAIEAPAMAAAAYPAPGYRTTVRIVPAALDV
                     YRPATTGRDAQPPGSR"
BASE COUNT          442 a          918 c          875 g          398 t
ORIGIN      
        1 gatcgagctg ccgtcgggct ccgacgtcag gggcgccatc gcgtacggca acctccgagc
       61 cgagggccgg ctcgccgcgt gcaccgtgaa gaccggtgcc ggcgacatca ccctcgacca
      121 ggccggcccc ctcgacgtcc gcaccggcgc gggcaccgtc tcgatcgccg tggcgatcgg
      181 gcactgcgac gtcgagagcg cctcgggcag cgtccgaatc cgccgggtgg aaggcagcgc
      241 cgcgatcaag aactcgtacg gtgacaccac gatcggcgag gtcaccggcg aactccgagt
      301 gaagggcgcc tacgggtcga tctgggtcga ccgtgcgctg ggctcggtcg acgtcaggac
      361 cgcccacggc aacgtccgcc tcgacgagat cgtgcgcggc gaagtgcagc tcgagagctc
      421 ctacggggcc ctcgaggtcg gggtccgcga gggcacggcc gtgtggctcg acgtggccac
      481 ccagcacggc agcgtcatca actcgctcgg cgcacatgac ggccccggag gagccgacga
      541 gaccgccgaa gtgcgggccc gaagctcgtg gggcgagatc gtcatccgcc gttccaacga
      601 acgggacacc gcgaacagga gcaactcatg aatcacgtga gaggagcaga cgtcatgagc
      661 aacatgtcga cgaccggacg gaacggtggg cccgcgatcc gcgtgcaggg tatcgagaag
      721 tcctacaatg atctgcacgt cctgcgcggc gtggacttcg aggtggctgc gggcagcatc
      781 ttcgccctgc tcggctcgaa tggggcgggc aagaccacga tcgtgagaat gctgtccacc
      841 ctcctcaagc aggacgccgg cacagccagc gtcaatgggt tcgacgtcgc aacgcaacct
      901 acgaaggtgc gagagtccat cagcctgacc gggcagttcg ctgcggtcga tgagatcctc
      961 accggtcggg agaacctggt gctggttgcc aggttgcggc atctgcagga tcccgcacgg
     1021 atcgcggatg agctgctcgc acggttcgac ctcaccgacg cggccacgcg gaaggtgtcc
     1081 acatactccg ggggaatgcg ccgtcggctg gacatctcga tgagcctcat cgggcagccg
     1141 aaggtgatct tcctcgacga gccgaccacg gggctcgacc ccgagggccg cctcgaggtg
     1201 tggcgggtca tccaggatct cgccacccgg ggcaccaccg tgctgctcac cacccagtac
     1261 ctcgacgagg ccgagcagct cgcggatcgg atcgcgatcc tgcaccaagg gcgaatcatc
     1321 gcgaacggca cgctcgccga gctgaagaag ctcctgcctc cggcgacggt cgagtacgtc
     1381 gagaagcaac ccaccctgga ggaagtgttc ctcaccctcg tgggcaacga cgacgggaac
     1441 cacgacacgg cacagacacg gaaggaccag tcatgagcac ccacttcttc gccgacaccg
     1501 ccctgctgac gggccggtcc ctgcggcaca tcctccgcag ccccgacacg atcatcacga
     1561 ccgcgatcac gccgatcgcg ctgctgctgc tgttcgtgta cgtgttcggc ggcgcgatcc
     1621 agaccggcac cgacgactac gtcacctacc tgctgcccgg gatcctgctg atcgcggtcg
     1681 cgtcgggcat cgcgtacacc gccttccggt tgttcctcga cctgcagtcc ggcatcttcg
     1741 aacgattcca ttccatgccg atcgcccggt cgtcggtgct gtgggcacac gtgctgacct
     1801 cgctggtggc caacgggctc acgctcgtga tcatcgtggg agtcgcgctg ctgatggggt
     1861 tccgcacctc ggcaggcctc cttgcgtggc tcgccgtcgc cgggatcctg gcgttgttca
     1921 ccctggctct gacctggctc gccgtgatcg cgggcctgac cgcgaagacc gtcgacggtg
     1981 ccagcggatt ctcctacgtg ctgatcctcc tgccgttcat cagctccgcg ttcgtcccca
     2041 cggacaccat gccgggtccg gtgcgcgcct tcgcggagaa ccagccggtc acctcgatcg
     2101 tcaatacgat ccaggacctg ttcgccggac aacccgtcgg caccgacatc tggatcgcgc
     2161 tcgcctggtg tgtcggcatc ctggtcatcg cctacgtcat cgccatggcg atctaccgac
     2221 gcaagatcgc atagcgacgt atcggatgag ctcgtcgaac cggccgggca gccctcagcg
     2281 gctgcccggc ggctgcgcgt cccgaccggt ggtggcgggc cggtacacgt cgagcgccgc
     2341 aggcacgatg cgaacggtcg tgcggtagcc gggcgcggga taggcggcgg cagccatcgc
     2401 gggcgcctcg atggcgacct cgccgtcgtg ggcgaatccc ggcggctgcc cctcgcgggg
     2461 tcgcacgacg acgtcgatcg attcggccgt gaaggactcg atgcgccgcg gaagcaatcg
     2521 cagcccgcgc agcaccgcgc tgatgcgggg gccgaacgcg agtgatgcgg cggcacgcgc
     2581 acgcgacccc gcgtgcagga tccgaacgtc gagcacgccc ccgtcgagcc gcc
//
LOCUS       DATDBZ010000435         4050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1786937, whole genome shotgun sequence.
ACCESSION   DATDBZ010000435 DATDBZ010000000
VERSION     DATDBZ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786937"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VIX62_10385"
     CDS             complement(<1..941)
                     /locus_tag="VIX62_10385"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEY5860682.1"
                     /translation="MRRARIAPTMVMIALTTILTMGPAMNALACGGLVAPNGTINLLK
                     TTTLAAYHRGIEHYVTSFEFAGQGAAEVGSIVPLPGVPTRVVKAGGWTLQRLVEEVTP
                     PPPELETADFAAAASPSAQVLLEAQIDALDITVLKGGAVAVGAWAKDHGFFLPPDAPE
                     VLEFYADRSPIFMAARFNAGRAEEQGVQTGEGTPIHVAIPTPNPWVPLRILGLGRQEG
                     ELIQADVFLLTDARPSTLPQAEDPSDDPDQRGLIQEVSEPASPSLLRDLRSDDRMRWL
                     PRSDLWLTYIRINEEAGRLTHDLAIDASGYGQPDPVAA"
     gene            1114..2541
                     /locus_tag="VIX62_10390"
     CDS             1114..2541
                     /locus_tag="VIX62_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY5860683.1"
                     /translation="MEVSTDHWIGGTRVSSAETFVDVSPIDEMPIAEVARGGAPEADR
                     AVAAAHEGFAVWGSMPAKERAEVLHRIADGVEARVPELAAVETRDNGSLLRSMRNSVM
                     PRVARNFRFFADHLLTLGEKKDHDGFAEQFEWDPSGVTAVITPWNAPLMLATWRVAPA
                     LAAGNAVILKPPEWAPLTASMLADIAHDAGLPDGAFNVLQGIGEEAGAALTAHPGLDR
                     IAFTGSVPTGKLVAQAAGANLTPASLELGGKSPFVAFADADLDAVVKQAVNQFDNAGQ
                     VCLAGTRLLVEASIHDEFMDRFTRAAAEIVQGDPRAAATEIGPQITREHFERVDGFVQ
                     RAKADGARAVFGAGPNDELGGLYYRPTLFTDAPTGSEILTQEVFGPVLTLQTFADEDE
                     AVALANDTRYGLAAILYTGDPGRAERVSAALVAGTVWVNCFFVRDLAAPFGGSRNSGI
                     GREGGTWSFDFYSDVKNVCTAPWTI"
     gene            2560..3459
                     /locus_tag="VIX62_10395"
     CDS             2560..3459
                     /locus_tag="VIX62_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HEY5860684.1"
                     /translation="MGEIVGAALVAHVPTIMLPEDTRRELNDGNEISLVPGLHRLKAE
                     ALDRLNPDTVIVFDTHWESTFEHIVTSAERRTGKFTSHELPRGMKQVPYDMPGDPELA
                     NAIAAQAEGRDDCWILPCDDPFLPIFYGTVNVWTFLGDADTRWMSVAINQCCTTEDFL
                     LFGELIGNAIRESDRRVVLLASGGMTHRFFSFQELRQHESSKAPDNILHRESYEADQR
                     VLAMMKDGDHAGIIDWMPEYRKAAPEGHFGHYLMMASAIGGARCQAPGEPYSEYEASV
                     GTGQVHVWFERPADGWTGEGTAA"
     gene            3509..>4050
                     /locus_tag="VIX62_10400"
     CDS             3509..>4050
                     /locus_tag="VIX62_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860685.1"
                     /translation="MADQPGQSQDMGYERLGEAKLSLVDVIAQSVGFMGPVFSAAFLI
                     PLIVGIISATGKGAGVAAPLSVLIASIGIFAIGWIVAQYAKKIHAAGSVYDYVSNGLG
                     NTIGAASGWLYYSGTMVLTAGLGVLVGWYIHDNIFTAVPEVPGVISSESPLPIWAWSA
                     IFAIAIFGVLYFGVQISTRV"
BASE COUNT          644 a         1341 c         1415 g          650 t
ORIGIN      
        1 gccgccaccg gatcgggttg gccgtacccg ctggcgtcga tcgctaggtc gtgcgtgagc
       61 cggccggcct cctcgttgat gcggatgtag gtgagccata gatcggatcg cggcagccac
      121 cgcatccgat cgtccgatcg gagatcgcgc agcagcgacg gcgatgccgg ctcgctcact
      181 tcctggatca ggccacgttg gtcaggatcg tcgctgggat cctcggcttg cggcagcgtg
      241 gagggccgcg catccgtcaa caggaacacg tcggcctgga tgagctcgcc ctcctgccga
      301 ccgagcccga ggatgcggag cgggacccag gggttcggcg tggggatcgc cacatggatc
      361 ggggtgcctt cgccggtctg caccccttgc tcttccgcgc gtcctgcgtt gaaccgggct
      421 gccatgaaga tcgggctccg atcggcgtag aactccagga cctcgggagc atcgggtgga
      481 aggaagaacc catggtcctt cgcccaggct ccgaccgcca cggcgccacc cttcaggacg
      541 gtgatgtcga gggcgtcgat ctgcgcttcg agcagcacct gcgctgacgg gctggcggcc
      601 gctgcgaagt ccgcggtctc cagctccggc gggggaggcg tgacctcctc gaccaaccgc
      661 tgcagggtcc acccaccggc cttcacgacc ctggtcggga cgcccgggag gggaacgatc
      721 gagcccacct cggccgcgcc ctggccggcg aactcgaacg aggtgacgta gtgctcgatc
      781 cctcggtggt aggccgccag ggtggtggtc ttgagcaggt tgatggtgcc gttcggcgcc
      841 acgagcccgc cgcaggcgag cgcgttcatg gccggtccca tcgtgaggat ggtggtcaag
      901 gcgatcatca ccatcgtcgg tgcgatccgt gcgcgacgca tcggagcccc tttcgtcgtt
      961 ggctctcgga cgccccgcga ggccggaccg gttccctgag gcttgacagc ggcttggcac
     1021 cggccatacc gttcgtgtcc gaacgacctt gggacgcacc gcgtcccacg acgcgaggag
     1081 agggtcgcca cggggcacgc cgtcgtcgag ggcatcgagg tctccaccga ccactggatc
     1141 ggcggcacgc gcgtctcgtc ggccgagacc ttcgtcgacg tctcgccgat cgacgagatg
     1201 cccatcgccg aggtcgcccg cgggggagcc cccgaagccg accgggccgt cgccgccgct
     1261 catgaggggt tcgccgtctg gggatcgatg cccgcgaagg agcgagcgga ggtcctgcac
     1321 cggatcgccg acggtgtcga ggcgcgcgtc cccgaactcg cggccgtcga gacacgggac
     1381 aacgggtcgc tgctccggtc gatgcggaac tccgtcatgc cgcgtgtcgc gcggaacttt
     1441 cggttcttcg ccgaccacct gctgacgttg ggtgaaaaga aggaccacga cgggttcgcc
     1501 gagcaattcg agtgggaccc cagcggtgtg acggccgtga tcacgccgtg gaacgctccg
     1561 ttgatgctcg ccacttggcg ggtggctccg gcgctggcgg ccggcaacgc cgtgatcctc
     1621 aagccgccgg agtgggcgcc gctcaccgcc tcgatgctcg ccgacatcgc gcatgatgcc
     1681 ggcttgcccg acggcgcatt caacgtgctg caggggatcg gggaggaagc gggtgccgcc
     1741 ctcaccgccc atcccgggct ggatcgcatc gcgttcaccg gatcggtgcc gaccggcaag
     1801 ctcgtggcgc aggccgccgg cgcgaacctc accccggcga gcctggagct cgggggcaag
     1861 tcaccgttcg tcgccttcgc cgacgccgat ctcgacgcgg tcgtgaaaca agcggtcaac
     1921 cagttcgaca acgccggtca ggtgtgcctg gccggtacgc ggctgctcgt ggaggcctcg
     1981 atccacgacg agttcatgga tcgattcacc agggccgccg ccgagatcgt gcagggcgac
     2041 ccacgagcag cggccaccga gatcggcccg cagatcaccc gcgaacactt cgagcgcgtc
     2101 gacggattcg tccaacgcgc caaggccgac ggtgcccgtg cggtcttcgg cgctggaccc
     2161 aacgacgagc tcgggggcct gtactaccgg cccacgctgt tcaccgatgc gccgaccggc
     2221 tccgagatcc tgacgcagga ggtcttcggc cccgtactca ccctgcagac gttcgccgac
     2281 gaggacgaag ccgtggcgct ggcgaacgac acgcggtacg gcctggccgc gatcctgtac
     2341 acgggcgatc cgggccgcgc cgaacgcgtg agcgcggcgc tcgtggccgg caccgtgtgg
     2401 gtcaactgct tcttcgtgcg cgacctggcg gccccgttcg gcggctcgcg caactccggg
     2461 atcggccggg agggcggcac gtggagcttc gatttctaca gcgatgtgaa gaacgtctgc
     2521 acggcaccat ggaccatttg atggagggga gagacgcaca tgggtgagat cgtcggcgcg
     2581 gcactggtcg cgcacgttcc cacgatcatg ctgccggagg acacgcggcg tgaactcaac
     2641 gacggcaacg agatctcgtt ggtgcccggc ctgcatcgac tgaaggccga ggcgctcgac
     2701 cggctgaacc cggacaccgt gatcgtgttc gacacgcact gggagtcgac cttcgaacac
     2761 atcgtgactt cggccgaacg ccggaccggc aagttcacga gccatgaact gccgcgcggc
     2821 atgaagcagg tgccctacga catgcccggc gaccccgagc tcgcgaacgc gatcgcggcg
     2881 caggcggaag ggcgcgacga ctgctggatc ctgccctgcg acgatccgtt cctgccgatc
     2941 ttctacggca ccgtgaacgt gtggacgttc ctgggtgacg ccgacacgcg gtggatgagc
     3001 gtggcgatca accaatgctg caccacggag gacttcctgc tgttcggcga gctgatcggg
     3061 aacgcgatcc gcgagagcga tcggcgcgtg gtgctgctcg cgagcggtgg catgacccac
     3121 cggttcttct cgttccagga gctccgacag cacgagagct cgaaggcgcc cgacaacatc
     3181 ctgcatcgcg agagctacga ggccgaccag cgcgtgctcg cgatgatgaa ggacggcgac
     3241 cacgcgggga tcatcgactg gatgccggag taccggaaag cggcgccgga ggggcacttc
     3301 ggccactacc tgatgatggc ctcggcgatc ggcggcgcgc gttgccaggc gccgggggag
     3361 ccctacagcg agtacgaagc ttccgtggga acgggccagg tgcacgtctg gttcgagcgg
     3421 cccgcggacg gttggaccgg cgaggggacc gcggcctagc tgcggcgacc ggtcggacgg
     3481 ggggaaccga acaggaaggg aggctccagt ggcagaccaa ccaggtcagt cacaggacat
     3541 gggctacgaa cggctcggcg aggcgaagct ctcgctcgtc gacgtgatcg cccagtccgt
     3601 cggcttcatg ggaccggtgt tctccgccgc gttcctcatc ccgctgatcg tggggatcat
     3661 ctcggcgacg ggtaagggcg ccggtgttgc cgcaccgctg tcggtcctga tcgcgtcgat
     3721 cggcatcttc gccatcggtt ggatcgtggc ccagtacgcg aagaagatcc acgccgccgg
     3781 gtccgtgtat gactacgtct ccaatggcct aggcaacacg atcggtgccg cgtcgggctg
     3841 gttgtactac agcggcacga tggtgctcac cgccgggctc ggtgtcctgg tcggttggta
     3901 catccacgac aacatcttca cggcggtgcc ggaggtgccg ggcgtcatca gttcggagtc
     3961 tcccttgccg atctgggcgt ggagcgcgat cttcgctatt gccatcttcg gcgtcctcta
     4021 cttcggtgtg cagatttcca cgcgggtgca
//
LOCUS       DATDBZ010000436         2007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2806385, whole genome shotgun sequence.
ACCESSION   DATDBZ010000436 DATDBZ010000000
VERSION     DATDBZ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806385"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VIX62_10405"
     CDS             complement(<1..274)
                     /locus_tag="VIX62_10405"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5860686.1"
                     /translation="MKITASAQRHIEAPPDRVYRYIANFRDHHPKFLPSQFSDFAVET
                     GGIGAGTVHRFNLTLGGRRSEYRVRVGEPEPGRVLIESDPSRLMLTT"
     gene            674..973
                     /locus_tag="VIX62_10410"
     CDS             674..973
                     /locus_tag="VIX62_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860687.1"
                     /translation="MSQRPPGFAPGTVLAQVSLRVIVPLLGGVIAGLVADGIGDTSPR
                     YVLIGLAGGTLISALWLRSFVASTVVQMRREREEAGATATEKHEPNRSEGNGGAT"
     gene            1002..1184
                     /locus_tag="VIX62_10415"
     CDS             1002..1184
                     /locus_tag="VIX62_10415"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY5860688.1"
                     /translation="MLLDLGFRLGLSVVIGVGAGVLLDNWLGTRPIFILLGVAVGIGA
                     AMYSIWDVARTEMRRR"
     gene            1207..>2007
                     /gene="atpB"
                     /locus_tag="VIX62_10420"
     CDS             1207..>2007
                     /gene="atpB"
                     /locus_tag="VIX62_10420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY5860689.1"
                     /translation="MPEEAEHGESLIHVAIKGEVLFHIGPIAVTNSMIGTLIATVTLI
                     ALAWWASRNSRVVPGRLQSFLEFPLEFLGGVIRDLQPTRFRAILPPILTIFMLVLFAN
                     YVGLLPGVGTINLHVVEEGAEASAPIIRPASADLNFTLALALISFVLFVSWGIRANGL
                     RGYLKETFISNPWYMTPLMTPIHIVSELSRIISLSMRLFGNIFAGEILLAVVLALTFA
                     VIPALFLGLEAIFGAVQALVFALLTLTYITLATAGHGGGHDEAHDAPAH"
BASE COUNT          307 a          668 c          679 g          353 t
ORIGIN      
        1 aagtggtgag catgaggcgg ctcgggtcgg actcgatcag cacgcgcccg ggctcgggct
       61 cgccgacccg cacgcggtac tccgagcggc gaccgcccag ggtgaggttg aatcggtgga
      121 cggtgcctgc gccgatgccg cccgtctcga ccgcgaagtc gctgaactgc gagggcagga
      181 acttcgggtg gtggtcgcgg aagttggcga tgtagcggta gacgcggtcc ggcggtgcct
      241 cgatgtgtcg ctgggccgac gcggtgatct tcatgcagca cagggtcgcc tgggccgcgc
      301 cacgggcgca tcggtcctgc gtcgggccgt cttctacgtc cttcgaccga gggatgtgaa
      361 ttgcgccggt ggtgaccgcc gacctggata actcggtgtg gtggagaaat tacgtggcgg
      421 gctgcgcccg acgactgcca ttccgagcat ggaacgcact cgactcagcc gcatctgccg
      481 cgtaacgctc cgaggacggc gggatatcca actcgggatg gcgcgactcg gacagcatgc
      541 gtccaccgag gcggtatcca actccggacc acccgccgcc cgcccgccga ccacggccgg
      601 ccgcgggcat gatccgggag cctcggctat actcgcgccg tccgaccccg ccgtgagtcg
      661 agctgtacac cgcatgtctc agagaccacc gggcttcgcg ccgggcacgg tgcttgccca
      721 ggtcagcttg cgagtgatcg tgccgctctt gggtggcgtg atcgcagggc tcgtggccga
      781 tggcatcggc gacacctccc cgcggtacgt cctgatcggc ctggcgggtg ggaccctcat
      841 ctcggcccta tggctgcgca gcttcgtcgc ctcgaccgtc gtgcagatgc gtcgcgagcg
      901 cgaggaggcg ggagccacgg ccacggagaa acatgaacca aaccgatccg aagggaacgg
      961 aggagcgacc tagccaggtc gagcgtcccc agaactgggg gatgctgctc gatctcgggt
     1021 tccgccttgg cctttcggtc gtcatcggcg tcggcgcggg agtcctgctc gacaactggc
     1081 tcggcaccag gccgatcttc atcctcctcg gggtggcggt cggcatcggt gcggcgatgt
     1141 attcgatctg ggacgtcgcg cggaccgaga tgaggcggcg gtagcacatg gagaagagga
     1201 tcctgcgtgc ctgaggaagc ggagcacggc gagagcctga ttcacgtcgc gatcaagggg
     1261 gaagtgctgt tccatatcgg cccgatcgcc gtcacgaaca gcatgatcgg cacgttgatc
     1321 gcgaccgtga cgctgatcgc cctggcgtgg tgggcgtcac gcaacagtcg cgtcgtcccc
     1381 ggccggctgc agtcgttcct cgaattcccg ctcgagttcc tcggtggcgt catccgcgat
     1441 ctgcagccca cgcgcttccg ggcgatcctg cccccgatcc tcaccatctt catgctcgtg
     1501 ctgttcgcca actacgtggg actgctgccg ggcgtcggga ccatcaacct gcacgtcgtc
     1561 gaggaaggcg ccgaagcgtc ggctccaatc atccggccgg catcggcgga cctgaacttc
     1621 acgctcgcat tggcgctcat ctcgttcgtg ctgttcgtga gctgggggat ccgcgccaac
     1681 gggctgcgcg gctacctcaa ggagacgttc atctccaacc cgtggtacat gacgccgctc
     1741 atgacgccga tccacatcgt gtccgagctg agccgcatca tcagcctgtc gatgcgactc
     1801 ttcggcaaca tcttcgccgg cgagatcctg ctggccgtgg tgctggcact gaccttcgct
     1861 gtcatcccgg cgctcttcct cggcctcgag gcgatcttcg gcgcggtgca ggcgctggtc
     1921 ttcgcactgc tgaccctcac ctacatcacg ctcgccacgg cggggcacgg cggcgggcac
     1981 gacgaggcgc acgatgcgcc ggcccac
//
LOCUS       DATDBZ010000437         2478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1363419, whole genome shotgun sequence.
ACCESSION   DATDBZ010000437 DATDBZ010000000
VERSION     DATDBZ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363419"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VIX62_10425"
     CDS             <1..699
                     /locus_tag="VIX62_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5860690.1"
                     /translation="GSSVLERSEEVVAKARMLAAHLLEVDEADLALGDGGLEVAGAPT
                     RSIAWPELASAAADRARLPAGMEPGLRAEGVYREPASTFPFGTHIAVVEVDTETGDVR
                     LVRHIAVDDCGRILNPMLVEGQVHGGLGQGIAQALYEEVRYDDAGNPMTGNLTTYLMP
                     SAAEFPWFETARTETPTPVNPLGAKGIGESATIGSTPAIQNAVVDALTPFGVRHLDLP
                     LSPERVWAALHPPG"
     gene            709..>2478
                     /locus_tag="VIX62_10430"
     CDS             709..>2478
                     /locus_tag="VIX62_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5860691.1"
                     /translation="MTDEGNPRWEARFRAPSVLMPDWSPTAPDRIVYESTESGVYQVH
                     CWDMATGAKRQVTDHPVGVVDGAPTLDGEGVLYWQDETGSEAGQWFIQPFAGGEGRPF
                     LQGTPQGWNQGFAQAPGVVVAAISDRDGFAVFAAVDGAPAKELVRSTEAVSLGGSTGG
                     GFNRGALSSDGTLLTLEHAEHGDLIHPALRVIDPRTGAVIDEQLDEGLALKSSCWSPI
                     AGDQRLAFLHERFGDERPAIWHLATGERTDLEIDLPGVIEVHDWWPDASALLLINLFE
                     GRDQVCRYDLTSGELTAIDHPAGTVWSARVRPDGGVWLRQSQGDRQALVLDDQGTEVL
                     RADGERAPEGRPYVSWHFDNGEGDRVHGFYVTPEGDGPFPVIMLVHGGPTWLDTDRFS
                     PEVQCYVDAGFVVGMVNYRGSIGYGRAWRDTLIGNIGGPEVVDVNAGLADLVARGIAD
                     PSRAVIGGWSWGGYITLMEVGTHPELWVAGIAGVPVGDYELSYDDMSPELQAYDRALL
                     GGAPADVPDLMRERNPINFAGRVTAPVMFIIGENDSRCPFRQAMAYVDRLKDRGHPHE
                     VYLFGTGHSSFDIEEEIHQQRAIL"
BASE COUNT          372 a          835 c          899 g          372 t
ORIGIN      
        1 gggtcgtcgg tcctcgagcg cagcgaagag gtcgtggcga aggcccgcat gctcgccgct
       61 catctgctcg aggtcgacga ggccgatctc gccctgggcg atggtggtct ggaggtggca
      121 ggagcgccga cccgctcgat cgcctggccc gagttggcgt cggccgccgc cgaccgtgcg
      181 cggctcccgg cagggatgga accggggctc cgtgccgagg gcgtgtatcg cgaacccgcc
      241 tcgaccttcc cgttcgggac ccacatcgca gtggtcgagg tcgacaccga gaccggggat
      301 gtgcgcctgg tgcgccacat cgccgtggac gactgcggac ggatcctgaa cccgatgctg
      361 gtggagggac aggtgcacgg cgggctgggc cagggcatcg cgcaggcgct gtacgaggag
      421 gtgcgctacg acgatgcggg caacccgatg accggcaacc tcaccaccta cctgatgccc
      481 tcggcggcgg agttcccctg gttcgagacc gcgcgcaccg agacgccgac cccggtgaac
      541 ccgctggggg ccaaggggat cggcgagtcc gccacgatcg ggtcgacacc ggcgatccag
      601 aacgcagtgg tcgacgcgct cacccccttc ggcgtgcgcc atctcgacct tccgctttct
      661 cccgagcgcg tgtgggccgc gctgcaccct cccgggtagg gttcctggat gaccgacgaa
      721 gggaaccctc gctgggaggc gcgattccgc gctccatccg tgctcatgcc cgactggtcg
      781 ccgacggcgc ccgaccgcat cgtctacgag agcaccgaga gcggcgtcta tcaggtgcac
      841 tgttgggaca tggccaccgg cgccaagcgg caggtgaccg atcaccccgt gggcgtcgtc
      901 gacggtgccc cgacgctcga cggtgagggc gtcctgtact ggcaggacga gaccggcagt
      961 gaggcaggac agtggttcat ccagccgttc gcgggtggcg agggccggcc cttcctgcag
     1021 ggcacgcccc agggctggaa ccaagggttc gcgcaggccc cgggggtcgt cgtcgcggcg
     1081 atcagcgatc gcgacgggtt cgcggtgttc gccgcggtcg acggagcacc cgccaaggag
     1141 ctggtccgca gcaccgaggc ggtgagcctg gggggctcca ccggcggagg attcaaccgt
     1201 ggcgcgctgt cctcggacgg aaccctgttg acgctcgaac atgccgaaca cggcgacctg
     1261 atccaccccg cccttcgcgt gatcgacccc cgaacgggcg ccgtgatcga cgagcagctc
     1321 gacgaggggc tcgccctgaa gtcctcgtgc tggtcgccga tcgcgggcga ccagcggctc
     1381 gcgttcctgc acgaacgttt cggcgatgag cgccccgcca tctggcacct cgcgacggga
     1441 gagcggaccg atctcgagat cgacctgccc ggggtgatcg aggtccacga ctggtggccc
     1501 gacgcgtcgg cgttgctcct gatcaacctc ttcgaaggcc gtgaccaggt gtgccggtac
     1561 gacctcacct ccggggagtt gacggcgatc gatcacccgg caggtaccgt ctggagcgcc
     1621 cgggtccgtc ccgacggcgg ggtgtggctg cggcagtccc aaggcgaccg tcaggccctg
     1681 gtgctggatg atcagggcac cgaggtgctg cgcgccgacg gtgagcgcgc ccccgagggc
     1741 cggccgtacg tgtcgtggca cttcgacaac ggcgaaggcg accgcgtgca cgggttctac
     1801 gtcacgcccg aaggcgatgg gccgttcccg gtgatcatgc tggtgcacgg cggcccgacc
     1861 tggctcgaca ccgaccggtt cagccctgag gtgcagtgct acgtcgatgc cgggttcgtg
     1921 gtgggcatgg tgaattaccg cggctcgatc gggtacggac gggcgtggcg ggacacgctg
     1981 atcggcaaca tcggcggccc cgaggtggtc gacgtcaacg cagggctcgc cgacctggtg
     2041 gcccgcggca tcgcggatcc gagccgcgcg gtgatcggcg gctggtcgtg gggcggctac
     2101 atcacgctga tggaggtcgg cacgcatccc gagctgtggg tcgccggcat cgcgggggtg
     2161 ccggtggggg actacgagct cagctacgac gacatgtcgc ccgagctgca ggcgtatgac
     2221 cgcgcgttgt tgggcggcgc gcccgccgac gtgcccgacc tgatgcgcga gcgtaacccc
     2281 atcaacttcg ccggccgggt caccgcgccg gtgatgttca tcatcggaga gaacgacagc
     2341 cgatgcccgt tccgtcaggc gatggcctac gtggaccgcc tgaaggaccg cggacatccg
     2401 cacgaggtct acctgttcgg caccggtcac tcctcgttcg acatcgagga ggagatccac
     2461 cagcagcgcg cgatcctc
//
LOCUS       DATDBZ010000438         2502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_549189, whole genome shotgun sequence.
ACCESSION   DATDBZ010000438 DATDBZ010000000
VERSION     DATDBZ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549189"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VIX62_10435"
     CDS             complement(<1..315)
                     /locus_tag="VIX62_10435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334724.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY5860692.1"
                     /translation="MDEFIAAVEVLRHPELRGRPVIVGGDGDPTKRGVVSTASYEARE
                     FGVRSGMPLRTALKRCPDGVFLAVDRDRYLEASGVVMDTLRSFPAVVQVLGWDEAFMA
                     VET"
     gene            complement(339..1538)
                     /locus_tag="VIX62_10440"
     CDS             complement(339..1538)
                     /locus_tag="VIX62_10440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY5860693.1"
                     /translation="MPESWIDMGLMVGSPPDPDKVVTYANWDKAPNQRWSFQHIREIA
                     PTARVARGETVSPLPRAERDLSGFSFRHRGEDHTLAGMMDETYVDGLMVVHDGTVVFE
                     HYVDGMTPSSTHLLQSVSKSMTAALTGVLVEQGRFDLNTTVPDHIEELRGTCWDGCTV
                     QQLLDMRAGTAFDETDYEDEDSESYRGFRMLGWLPRLPDDLMPADYIAQMQNDKEHGG
                     DFEYRSILTDVLGWCMERATGQHLADLFSQEVWAPMGAAHDADFMVGPSGFPLADGGF
                     CVSLADLARFGLMFLHGGEIAGRQVIPAQWVERLRIPDDDLIAVFAASPETGGYPKGA
                     FYHDQWWVRDAEAGIYSGYGINGQQVLVHHPSNTVVARMSSWPHPWVDEYAELADAGV
                     FALCEGL"
     gene            complement(1572..2243)
                     /locus_tag="VIX62_10445"
     CDS             complement(1572..2243)
                     /locus_tag="VIX62_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860694.1"
                     /translation="MTEQRSFKLLVRTRMEKTGESYTAARAALLGGATDPATDGGKAP
                     LAVSDESIRERTGRGWEEWFDALDEWGAQDRPRRDIARWVAEQLTIEPLGWNAQAVTM
                     SYERARRGRAVGEHPDGFRAGVTKTVAVPVDRLFDAFVDSSLRKRWLPDGQLRDRTAT
                     KPKAAHFDWADGPSRVHVTFVAKEADKSTATVSHERLTDAAERDRMKAYWKHAMDALE
                     LELER"
BASE COUNT          392 a          859 c          827 g          424 t
ORIGIN      
        1 ggtctccacc gccatgaacg cctcgtccca cccgagcact tggacgacgg ccgggaacga
       61 tcgcagggtg tccatgacca cgccgcttgc ttccaagtag cggtcacgat cgaccgccag
      121 gaacaccccg tcgggacaac gcttcagcgc ggtgcgaagg ggcatgcccg atcgcacgcc
      181 gaactcccgg gcttcgtagg aggccgtgga gaccacgccg cgtttcgtgg ggtcgccgtc
      241 acccccgacg atcaccggcc tgccgcgcaa ctccggatgc cgcagtacct cgaccgcggc
      301 gatgaactcg tccatatcga cgtggaagat ccaacgggtc acagcccctc gcacagggcg
      361 aacacgcccg cgtcggcgag ttcggcgtac tcgtccaccc acgggtgggg ccaactggac
      421 atgcgggcga ccacggtgtt ggaagggtga tggaccagta cctgttgacc gttgatgccg
      481 taaccagagt agatgcccgc ctctgcgtct cgtacccacc actggtcgtg atagaaggcg
      541 cccttggggt agccgcccgt ttcgggcgaa gcggcgaaca cggcgatgag gtcgtcgtcg
      601 gggatgcgca gccgctcgac ccactgcgcg gggatcacct ggcggccggc gatctcgcca
      661 ccatgcaaga acatcagccc gaaccgagca aggtcggcca gcgacacgca gaaccccccg
      721 tcggcgaggg ggaatcctga aggtcccacc atgaagtcgg cgtcgtgggc ggcgcccatc
      781 ggcgcccaca cctcctgtga gaacaggtcc gcgaggtgct ggccggtcgc acgctccatg
      841 caccagccca agacgtcggt caggatcgat cggtactcga agtcgccgcc gtgctctttg
      901 tcgttctgca tctgggcgat gtagtcggcg ggcatgaggt cgtcgggcag tcgcgggagc
      961 caccccagca tccggaaccc tcggtaggac tccgaatcct catcttcgta gtcggtctcg
     1021 tcgaacgcgg tgccggcacg catgtccaag agttgctgca cggtgcagcc atcccagcag
     1081 gtgccgcgaa gctcctcgat gtggtcgggc accgtcgtgt tcaggtcgaa ccggccctgc
     1141 tcgaccagca ccccggtcaa cgccgccgtc atcgacttcg agaccgattg cagcaggtgg
     1201 gtcgacgatg gggtcatgcc gtcgacgtag tgctcgaaca cgaccgtgcc gtcgtgaacg
     1261 accatgaggc cgtcaacgta ggtctcatcc atcatgccgg cgagcgtgtg atcctcacct
     1321 cggtggcgga acgagaaccc cgacaggtct cgctcggcac gcggcagggg cgagacggtc
     1381 tcgcctcggg ccacgcgcgc tgtcggcgcg atctcgcgga tgtgctggaa cgaccagcgc
     1441 tggttcggcg ccttgtccca gttcgcgtag gtcacgacct tgtcggggtc gggcggcgac
     1501 cccaccatca accccatgtc gatccagctc tcgggcatgc gtgctccacc ttcctcgcga
     1561 tcgtggtcgg ttcatcgttc cagctccagt tccaaggcgt ccatcgcgtg cttccagtac
     1621 gccttcatgc ggtcgcgttc ggcggcgtcg gtgaggcgct cgtgggacac ggtcgccgtg
     1681 ctcttgtccg cctctttggc gacgaacgtg acgtggaccc gggaaggtcc gtcggcccag
     1741 tcgaaatggg cggccttcgg cttcgtcgcc gtgcggtcgc ggagctgtcc gtcggggagc
     1801 caccgctttc ggagcgagct gtcgacgaac gcgtcgaaga gtcggtcgac gggtaccgcc
     1861 acggtcttcg tgacgccggc gcggaagccg tcggggtgct cgcccacggc gcggccacga
     1921 cgggctcgct cgtagctcat ggtgaccgcc tgggcgttcc aacccagcgg ctcgatcgtg
     1981 agttgttcgg cgacccacct cgcgatgtct cggcgcgggc ggtcctgagc gccccactcg
     2041 tcgagcgcgt cgaaccattc ctcccaccct ctgccggtgc gttcgcggat cgactcgtcc
     2101 gagacggcga gcggtgcctt gccgccgtcg gtcgctggat cggtcgcgcc gcccaacagt
     2161 gcggcccgcg ccgcggtgta gctttcgccc gtcttctcca tgcgcgtacg gacgaggagc
     2221 ttgaaagatc gttgctcggt catctcgtgc ttcccttcat ggcggcgatc ccgcaactca
     2281 cgctcgcgac acccccgccg gacagggcta gcactggacc ggcgtcccga gggctcgagg
     2341 ccccctttgc ccccgctggg ccggcggcgt gagcgccgga actcgagggt tgggcgcagg
     2401 acggcgccga tccccaacgc taccgccgac ccactcatct cgcaagtttc gttcggggcg
     2461 gagtagggca tcatggaggg aaccggaagg aggcagtcat gt
//
LOCUS       DATDBZ010000439         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_345717, whole genome shotgun sequence.
ACCESSION   DATDBZ010000439 DATDBZ010000000
VERSION     DATDBZ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345717"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VIX62_10450"
     CDS             <1..692
                     /locus_tag="VIX62_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY5860695.1"
                     /translation="DRDALLARVRGVTDVGAFTDVDLVIEAATEDPQIKLETFRRLDE
                     VVRPGVVLASNTSSIPIATLGAVTGRPDAVIGMHFFNPPPVMGLVELTPSLSTSDDTV
                     EFARGFGERVGKTVVVAKDHAGFIVNRLVIPSICAAIRLYDEGFASREDIDVAMTLGM
                     NHPMGPLRLADFIGLDTVLQIAEVLHAEFRDPMYAPPPRLQRMVDAGHLGRKRGRGFY
                     DYGQRPAGDTT"
     gene            694..1596
                     /locus_tag="VIX62_10455"
     CDS             694..1596
                     /locus_tag="VIX62_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5860696.1"
                     /translation="MGHSHAPAQSASARHIGRLKAVFAITFVVMIAEVVAGVIAGSLS
                     LLADAGHMLTDIAGIGLALLAIYLAARPATEGRTFGWYRLEILAAVVNAMLLFGVAIW
                     VLYEAWQRFSDPPEVRSGLMLIVATIGLVANGVSARLLLEGQRESLNMRGAYLEVLGD
                     LLGSVAVIAAAVVIATTGYEAADPIASVVIGFLILPRTWRLLREAVDVLLEATPNDVD
                     LDEVRRHIVEMDGVADVHDLHAWTITSGMNVVSAHVVMAEGADASGVLDRLGTCLSGD
                     FDIEHSTFQLEPSDRRTIEETTHR"
     gene            complement(1612..>2004)
                     /locus_tag="VIX62_10460"
     CDS             complement(1612..>2004)
                     /locus_tag="VIX62_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY5860697.1"
                     /translation="STGKRDYYSVFHRLADEAAFKRAMLVCAINNMPGLSFPAQYSSV
                     FSVASHEDVDDPFAFDFNPSPPAEWGAPGIDVEVPWLDQKTIVATGNSFAAPHISGIL
                     TLILSKHPELTPFQAKTVLMALARNTHS"
BASE COUNT          310 a          648 c          695 g          351 t
ORIGIN      
        1 ccgaccggga cgcgctgctc gctcgggtgc gaggggtcac cgacgtcggg gccttcaccg
       61 acgtcgatct ggtgatcgag gccgccaccg aggatccgca gatcaagctc gagaccttcc
      121 gcaggctgga cgaggtggtc cgccccggtg tcgtgctcgc atcgaacacg tcctcgatcc
      181 cgatcgcgac gctgggcgcg gtcacgggcc gacccgacgc ggtgatcggc atgcacttct
      241 tcaaccctcc gcccgtgatg gggctcgtcg agctcacgcc gtcgctctcc accagcgatg
      301 acacggttga gttcgctcgc gggttcggcg agcgagtggg caagaccgtg gtggtggcca
      361 aggatcacgc gggcttcata gtgaaccgct tggtgatccc ctccatatgc gcggcgatcc
      421 gtctgtacga cgaggggttc gcgagccgcg aggacatcga tgtcgccatg accctgggca
      481 tgaatcaccc catgggcccg ctgcgcctcg cggacttcat cgggctcgac accgtgctac
      541 agatcgccga ggtcctgcac gcggagttcc gcgacccgat gtacgcgccc ccgccgcggt
      601 tgcagcgcat ggtcgacgcg ggacacctgg gacgcaagag gggtcgcggg ttctacgact
      661 acggccagcg acctgccggg gacacgacct agggtgggtc actcgcacgc gcccgcccag
      721 agcgcgtcgg ctcgacacat cgggcgcctg aaggcggtgt tcgcgatcac gttcgtcgtg
      781 atgatcgcgg aggtcgtcgc cggggtcatc gcggggagcc tgtcgctgct cgccgatgca
      841 gggcacatgc tcaccgacat cgcggggatc gggctggcgc tgctcgcgat ctatctcgcc
      901 gcgcggcccg cgaccgaagg acgaacgttc ggctggtacc ggctggagat cctggcggcc
      961 gtcgtcaacg ccatgctgct gttcggcgtc gcgatctggg tgctctacga agcctggcag
     1021 cggttctccg acccgcctga ggtccgcagc gggctcatgc tgatcgtggc gaccatcggc
     1081 ctggtcgcga acggtgtgtc cgccaggttg ttgctcgaag gccagcgaga gagtttgaac
     1141 atgcgtggcg cctacctgga ggtgctcggc gatctcttgg gctcagtggc cgtgatcgcg
     1201 gcggccgtgg tgatcgcgac cacgggttat gaggctgccg accccatcgc ctcggtcgtg
     1261 atcggcttct tgatcctgcc ccgcacctgg cgtctattgc gtgaggccgt cgacgtgttg
     1321 ctggaggcca cgccgaacga cgtcgatctg gacgaggtgc gccggcacat cgtggagatg
     1381 gacggggtcg ccgacgtcca cgacctgcac gcctggacga tcaccagtgg catgaacgtg
     1441 gtctcggccc acgtggtcat ggccgagggc gccgacgcga gcggcgtcct cgatcgcctc
     1501 ggcacctgtc tgtcgggtga cttcgacatc gagcacagca cgttccagct ggagccgtcc
     1561 gaccgcagga cgatcgaaga gaccacacac cgctagctgc ccggctcctg ctcaggagtg
     1621 ggtgttgcgg gccaacgcca tgagcacggt cttggcttgg aacggggtca gctccgggtg
     1681 cttggacagg atcagggtga ggatccccga gatgtgtggt gccgcgaacg agttgccggt
     1741 ggccacgatc gtcttctgat cgagccacgg cacctcgaca tcgatgccgg gcgcacccca
     1801 ttccgccggg ggtgacgggt tgaagtcgaa cgcgaacggg tcgtcgacgt cttcgtgcga
     1861 tgccaccgag aacaccgacg agtactgcgc cgggaagctc aaccccggca tgttgttgat
     1921 ggcgcacacc agcatcgctc gcttgaacgc cgcttcgtcg gcgagtcggt ggaacaccga
     1981 gtagtagtcg cgcttgcccg tgga
//
LOCUS       DATDBZ010000440         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_771747, whole genome shotgun sequence.
ACCESSION   DATDBZ010000440 DATDBZ010000000
VERSION     DATDBZ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771747"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1797
                     /locus_tag="VIX62_10465"
     CDS             244..1797
                     /locus_tag="VIX62_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5860698.1"
                     /translation="MTRTVASLIAGKPVDASATTLTIPNPANLDVVVAEAALADADTF
                     LEACRVAKDAQRGWAATPAPVRGRAIQQFGRLVEDNFEALSALITREIGKPIAESRGE
                     VQEVIDTCNFFLGEGRRLYGQTVPSEMPDKQLFTFRTPVGVAAIVTAGNFPVAVPSWY
                     IVPALVCGNAVVWKPAEYTPATAEAFAQLLLHAGLPEGVFNLVLADGTQTFEGLERAL
                     DEGVLDKIGFTGSSDVGRRIGELAGRHLQNPCLELGGKNPLVVMPDADLDLAVEGALF
                     SGFGTAGQRCTSLGTAIVHASIHDEFVTRFDAAVSGAAIGDPTQAVLYGPMISQRFAD
                     RFEQWLELIEPHQRVLGSTGTGRIAAGNPRERFVGDAEAGLFYHPTIVDGVAIEDDLY
                     GTETFGPIVGVASFGDFDQAMALANGHGYGLSSSIYTRDPLHAFRFRERISAGMVSVN
                     NSTSGAEAHLPFGGNGKSGNGSRQSGVWVLDQFTKWQSMNWDYSGHLQKAQMDVADVE
                     ADLSFRLPD"
     gene            complement(1794..>2138)
                     /locus_tag="VIX62_10470"
     CDS             complement(1794..>2138)
                     /locus_tag="VIX62_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860699.1"
                     /translation="ANGDSVGVGTTLAEALAESVKGQVDTGGGEPGGGGGGNEETVND
                     LLNQALDHFQAAQEALTAGDLATYQDELDQAQQLVEQANELAGQQADGATGATGVTGS
                     IASPSSTASPSP"
BASE COUNT          332 a          747 c          735 g          324 t
ORIGIN      
        1 agagccgggc gacgatcggc gaggccgcgc gggtcgccac cgccggagat ccagggccgg
       61 ctggagaccg cagggcgatc gcgccgccga tcgttaggcc ggcagcggcc acgaccgcca
      121 gcacgccgat ccaccacgtc atcggacgac ccatgccgta ggcatcggct gagaccccac
      181 ggtggttgac ggccgcccgt tccgggagac gtgcgtccag ccgctcaggg ctaccatgcc
      241 tgcatgaccc gaaccgtggc atcgctcatc gcaggcaagc cggtcgacgc atcggccacc
      301 accctcacga tcccgaaccc ggcgaacctc gatgtggtcg tggcagaggc cgcgctcgcg
      361 gacgccgaca cgttcctcga ggcgtgccga gtggccaagg acgcccagcg tggctgggcc
      421 gccacccccg cacccgtgcg cggccgggcg atccagcagt tcgggcggct ggtggaggac
      481 aacttcgagg cgctcagcgc actgatcacg cgcgagatcg gcaagccgat cgcggagtcg
      541 cgcggcgagg tgcaggaggt catcgacacc tgcaacttct tcctgggtga gggcagacgg
      601 ctctacggcc agaccgtgcc gagcgagatg ccggataagc agctcttcac gttccgcacg
      661 ccggtgggcg tcgcggccat cgtcaccgcc gggaacttcc ccgtcgcggt cccgtcctgg
      721 tacatcgtgc cggcgctcgt gtgcggcaac gcggtcgtat ggaagccggc cgagtacacc
      781 cccgccaccg ccgaggcgtt cgcccagttg ctgttgcacg ccggcctgcc cgagggcgtg
      841 ttcaacctgg tgctggccga cggcacccag acgttcgaag ggctcgagcg cgcgctcgac
      901 gagggcgtgc tcgacaagat cggcttcacc ggctcgagcg acgtgggacg gcgcatcggc
      961 gaactcgcgg ggcggcacct gcagaacccg tgcctcgaac tgggcggcaa gaatcccctc
     1021 gtggtcatgc ccgacgccga cctggacctg gcggtggagg gcgcgctgtt cagcgggttc
     1081 ggcacggccg gccagcgctg cacgtcgctc ggcaccgcca tcgtccacgc atcgatccac
     1141 gacgagttcg tcactcggtt cgatgccgcg gtcagtggcg ccgcgatcgg tgacccgacg
     1201 caggccgtgc tgtacggacc gatgatctcc cagcgcttcg cggaccgctt cgagcagtgg
     1261 ctggagttga tcgagccaca tcagcgggtg ctgggctcca cgggcaccgg gcggatcgcc
     1321 gccggcaatc cacgcgaaag gttcgtcggc gacgccgaag ccgggctctt ctaccacccg
     1381 acgatcgtgg acggggtcgc gatcgaggac gatctctacg ggacggagac gttcgggccg
     1441 atcgtagggg tggcgtcgtt cggcgacttc gaccaggcga tggccctggc gaacggccac
     1501 gggtacggcc tgtcgagcag catctatacg cgcgatcccc tgcacgcgtt ccggttccgc
     1561 gagcggatct cggccggcat ggtcagcgtc aacaactcca ccagcggcgc tgaggctcac
     1621 ctgcccttcg gcggcaacgg caagagcggc aacggctcgc ggcaatccgg tgtgtgggtc
     1681 ctggatcagt tcacgaagtg gcagtcgatg aactgggact acagcggtca cctgcagaag
     1741 gcgcagatgg acgtcgccga cgtggaagcc gacctctcgt tccgtctgcc cgactagggg
     1801 ctgggactag cggtcgagct gggcgaagcg atcgaaccgg tcactcccgt cgcgcccgtg
     1861 gcaccatcgg cctgctggcc cgcgagttcg ttcgcctgct cgacgagctg ttgcgcttgg
     1921 tcgagctcat cctggtaggt cgcgaggtcg ccggcggtca gcgcctcctg ggcggcctgg
     1981 aagtgatcga gcgcctggtt caacagatcg ttgaccgtct cctcgttccc accgccaccg
     2041 cctccaggtt caccgccgcc ggtgtcgacc tggcccttca ccgattcggc gagcgcctcc
     2101 gccaaggtgg tgccgacccc caccgagtca ccgttcgc
//
LOCUS       DATDBZ010000441         2023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2605690, whole genome shotgun sequence.
ACCESSION   DATDBZ010000441 DATDBZ010000000
VERSION     DATDBZ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2605690"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..293
                     /locus_tag="VIX62_10475"
     CDS             159..293
                     /locus_tag="VIX62_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HEY5860700.1"
                     /translation="MLWVIAAVLVVAGIVSIVRGALLWGIVLIIGGLLVGPGGVSILD
                     "
     gene            306..1061
                     /locus_tag="VIX62_10480"
     CDS             306..1061
                     /locus_tag="VIX62_10480"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860701.1"
                     /translation="MLADINVEQGLEEAWADVVTFAPKLLGFFVILLIGYFIAKALSK
                     LANTLLERVGFDGLVERGTMRQAFERSKTDPSDVIGVVVFWLVFLVALQLAFGIWGPN
                     PISDLLEGLIAYLPNVIVAVVILVIAAVLARALTDILTPMLGAVRGGSIIAKGAGIAI
                     LVVGIFAALDQLQVAPAIVTGLFYALLVMVVGSAVVAFGVGGIPIARRYLERWSGSVE
                     ATARDVKAEVQSRTTEGDEATMPMPPVASDPAS"
     gene            1106..1291
                     /locus_tag="VIX62_10485"
     CDS             1106..1291
                     /locus_tag="VIX62_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002481109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY5860702.1"
                     /translation="MGEHLDKAKGTVKENVGDATDNERLEREGKMDRAGADVKEKAND
                     AVDTVKDKVNDLTDRDD"
     gene            1419..>2023
                     /locus_tag="VIX62_10490"
     CDS             1419..>2023
                     /locus_tag="VIX62_10490"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY5860703.1"
                     /translation="MTLPVRGPLSAAAAALLRGEPVMPPPVAVADPLTDDDLHLALFL
                     CYEVHYRSLEGAADLAEWDPRVLAFRAELERCFHDALGGVAFVAPTSDPVDVQLRRLI
                     DADGAPSVSSFLAREGTVAMYREFLMHRSAYHLKEADPHTFAIPRLRGRAKAAMVEIQ
                     ADEYGGGDARWMHSTLFARTMHELGLATEEGAYVEQLPGVTL"
BASE COUNT          303 a          699 c          688 g          333 t
ORIGIN      
        1 acatatgtcc atgtctgcgt ttggccagcc ccgcgactgg aaagggtcct cttcgacatc
       61 agaccgcgcg ctcgttcgcg cacgagggag gaaccatgtt gcagaccggg ctcgcgctcc
      121 tggcgcagaa cggcgggaac gaaggcacca ccgacacgat cctgtgggtc atcgcagccg
      181 tgttggtcgt cgccggcatc gtcagcatcg tccgcggggc gttgctgtgg gggatcgtgc
      241 tgatcatcgg tggcctgctc gtcggccccg gcggcgtcag catcttggac tgaggggagc
      301 ctcacatgct cgcagacatc aacgtcgagc aggggctcga ggaggcctgg gccgacgtcg
      361 tgacgttcgc gcccaagttg ctcggcttct tcgtcatcct gctgatcggc tacttcatcg
      421 cgaaggcgct gtcgaagctc gccaacacgc tgctcgagcg ggtcggcttc gacggcctcg
      481 tggaacgcgg cacgatgcgc caggcgttcg agcgcagcaa gaccgaccca tccgacgtga
      541 tcggcgtcgt ggtcttctgg ctcgtgttcc tggtcgcgct gcaactcgcg ttcggcatct
      601 ggggtcccaa ccccatcagc gacctgctgg aaggcctgat cgcgtacctg cccaacgtga
      661 tcgtcgccgt ggtgatcctc gtgatcgccg cggtgctcgc gagggccctg accgacatcc
      721 tgacgccgat gctcggcgct gtgcggggcg gttcgatcat cgcgaagggg gccggcatcg
      781 cgatcctggt cgtcggcatc ttcgccgcac tcgaccagct ccaggtcgcg ccggccatcg
      841 tcaccggcct cttctatgcg ctcctggtca tggtcgtcgg ttcggcggtc gtcgcgttcg
      901 gcgtgggcgg catcccgatc gctcgccgtt acctcgaacg ttggagcggc tcggtcgagg
      961 cgaccgcgcg cgacgtgaag gccgaggtcc agtcgcgcac caccgagggc gacgaggcca
     1021 ccatgccgat gccgccggtg gcttccgacc cggcttcctg accgatccgg acccgaggca
     1081 actccgaccg aaaggagccc acaccatggg cgagcacctg gacaaggcga agggcaccgt
     1141 caaggagaac gtcggtgacg ccaccgacaa cgagcgtctc gagcgcgagg gcaagatgga
     1201 ccgcgccggg gccgatgtga aggagaaggc gaacgacgcc gtcgacaccg tgaaggacaa
     1261 ggtcaacgac ctgaccgatc gcgacgactg acggcatccg tcgcgatccg accccgccac
     1321 cccccggcgg gttcccgacg acccgcccgg ctctgcgccg ggcgggtcgt cgtcgctctg
     1381 ggagtttggg gggcccgcac cccgggaatc tcagcgccat gaccctgccc gtgcgaggac
     1441 ccctcagtgc cgcggcagcg gccttgttgc gcggcgaacc ggtgatgccg ccgccggtcg
     1501 ccgtcgccga cccactcacc gatgacgacc tgcacctggc gctcttcctg tgctacgagg
     1561 tgcactaccg ttcgctcgag ggggccgctg atctcgccga gtgggatccg cgggtcctgg
     1621 cgttcagggc cgagctcgag cggtgcttcc acgacgccct gggcggtgtc gccttcgtgg
     1681 cgccgacatc ggaccccgtc gacgtgcagc tgcggcgcct gatcgacgcg gacggggcac
     1741 cctcggtctc ctcgttcctc gcccgcgagg gcaccgtggc gatgtatcgg gagttcctga
     1801 tgcaccgatc tgcctatcac ctgaaggaag ccgacccgca cacgttcgcg atcccccggc
     1861 tccgtggccg ggccaaggcc gccatggtgg agatccaggc cgacgagtac ggcggcggcg
     1921 atgctcggtg gatgcactcc acgctgttcg cacggacgat gcatgagctc gggctcgcga
     1981 ccgaggaggg cgcctacgtc gagcagctgc ccggcgtgac cct
//
LOCUS       DATDBZ010000442         4470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2808711, whole genome shotgun sequence.
ACCESSION   DATDBZ010000442 DATDBZ010000000
VERSION     DATDBZ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2808711"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VIX62_10495"
     CDS             complement(<1..334)
                     /locus_tag="VIX62_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HEY5860704.1"
                     /translation="MQAEPNRRLAYEEILVVLMLSLLVSSVGAILSLLEAPLRGVVVP
                     SVSQDPRLARQVADFIFDLAPVFLVVYLVRRSGEGVGTIGLAWDRPRQDVLRGVALFA
                     VVGLAGLGL"
     gene            406..1626
                     /locus_tag="VIX62_10500"
     CDS             406..1626
                     /locus_tag="VIX62_10500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5860705.1"
                     /translation="MAKGESADLVVVGAGTVGGWASVFASEAGLKKVVVLEADVVGGG
                     ASSRAAGMVRAQGGTPDTVRLGAWSIDWYRGQGARYGTDSGFQGRGYVILAVTAADER
                     AAHERIAMQRATDLDARWVDADEVRALIPAMGSTGFRGGSYVATDGWVDPPRNVRAYS
                     LAMQQRGVEVRERTAFVGLRTRAAGRGRREVTGVRTTGGTIATSRVLLTGGPAMQAVA
                     TAAGARAWVGYARHQVVVTEASPGLHADTTAMAFDIGAGIYWRPEEGGLLWGMSNPDE
                     EPGPGRSIDWPYLRRMERRLHRLVPVTRTLGIKKAWAATIEYTPDHFPLSGPLVLRDG
                     TEVAGASIASACGHGMMWGPAVSRIAVDLALAGATDVVEAAEAFRMDRFDEHGTPPSV
                     DPVALPFPVHVDDA"
     gene            complement(1646..2002)
                     /locus_tag="VIX62_10505"
     CDS             complement(1646..2002)
                     /locus_tag="VIX62_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860706.1"
                     /translation="MSDPGPSGPSAKPVARRRRTLIVLVAAGLSLAAITVALIAAGET
                     EPSARVFDVAAGDRRYWAPGVLRPGDQMRCHGEVYDLWHAGPDDSVAFGDTGITVGHA
                     GDGSIIATCPKTLPAP"
     gene            complement(2050..2283)
                     /locus_tag="VIX62_10510"
     CDS             complement(2050..2283)
                     /locus_tag="VIX62_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY5860707.1"
                     /translation="MVNAYILIQTEVGKAAQVTEQIRVVPGVVAVDPVTGPYDVVARA
                     EADDLDQLAKSTVMPMQELEGITRTLTCPVLQL"
     gene            complement(2318..2653)
                     /locus_tag="VIX62_10515"
     CDS             complement(2318..2653)
                     /locus_tag="VIX62_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860708.1"
                     /translation="MSDDQGFIDKAKGTAGDLAGKAKGTAGDLAGKAGPAVDKAKDTA
                     GGVVDAAAPVAGKAVGAAGEVAGKAVGAAGDLAGKTVDAAAPVAGKAKDTAGGLLDKV
                     KGLFGGGKS"
     gene            2821..4137
                     /gene="melA"
                     /locus_tag="VIX62_10520"
     CDS             2821..4137
                     /gene="melA"
                     /locus_tag="VIX62_10520"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY5860709.1"
                     /translation="MAKVTIIGAGSVEFTRNILADLCSFTELHGTLTIALHDIDAERL
                     AYAERAANQLVERFDAGYRVGAHADRAGAFDGADYLINEIQVGGYRATVTDFEVPSRF
                     GVRQTIADTIGIGGIMRGLRTIPVMVEMADEIAQRSADGSLLLNYTNPMAMVPWGIWA
                     GSSLPADRVIGVCHSVRDTHTFLAETVGVPEADIRFVTAGFNHQCFVYTFEDRRTGKD
                     LYPRLREIVEADPEGLGRRVRVELFRRFGYFPTESSEHSSEYVPWFLHLDDQVAHFRC
                     EIDEYIRRSDENLVEFAETKRALDAGEELEVELNDELASQIVRAIETGTERDVYTNIR
                     NDGRIDGLPTEACVEVPTTVGAEGVRPHAVGALPVQTLALNRTFLNVVELTVRAALEQ
                     RRDLVEAAALLDPNTGATLSTAAIHEMVDALITAHGDLIPEGIRRG"
     gene            complement(4156..>4470)
                     /locus_tag="VIX62_10525"
     CDS             complement(4156..>4470)
                     /locus_tag="VIX62_10525"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860710.1"
                     /translation="DRRIEDVCEWRGFALVSDLEVMPSHRRLGAGRALMEALEAEALG
                     AGMPGVALDTGTEESFAEARGLYRSMGYIDRGEVFLGGWSDPLVPGRHFVDPLTQWFK
                     RF"
BASE COUNT          677 a         1563 c         1560 g          670 t
ORIGIN      
        1 agagcccaag gcccgcgagt ccgacgaccg cgaacaacgc cacgccacgc aggacgtcct
       61 ggcgcggccg gtcccacgcg aggccgatcg tgccgacacc ctcgcccgag cgacgcacca
      121 ggtacaccac gaggaagacc ggggctaggt cgaagatgaa gtcggctacc tgtcgggcca
      181 gcctcggatc ttgactcacc gatgggacga ccacgcctcg tagcggcgcc tccaagagac
      241 tgaggatcgc gcccaccgaa ctcaccagga gcgagagcat caggacgacg aggatctctt
      301 cgtacgcgag gcggcggttg ggttcggcct gcatcggcgc cactgtacgc atgaccagag
      361 cgagggttcg ctcctctaga cttcgacctt ctccaggagg gtcgagtggc gaagggcgag
      421 tctgcggatc tggtggtggt gggtgccggg accgtcggtg ggtgggcgtc ggtgttcgcc
      481 tcggaggccg gcttgaagaa ggtcgtcgtg ctggaggccg acgtggtggg cggcggagcc
      541 tcgagccgcg cggcaggcat ggtgcgggcc cagggtggca ccccggacac cgtccgcctg
      601 ggcgcgtggt cgatcgactg gtatcgcggc cagggcgctc ggtacggcac cgactccggg
      661 ttccagggcc gcgggtacgt gatcctcgcc gtcaccgctg ccgacgagcg agcggcacac
      721 gaacgcatcg cgatgcagcg cgcgaccgac ctcgacgcac ggtgggtgga cgccgacgag
      781 gtacgagcgc tgatcccggc gatggggtcc acggggttcc gcggtgggtc ctacgtcgcc
      841 accgacggat gggtcgatcc accgcgcaac gtgcgcgcct actccctcgc gatgcagcag
      901 cggggcgtcg aggtccggga gcggaccgcg ttcgtcggtc tgcgcacccg tgccgccggt
      961 cggggacggc gcgaggtcac cggggtgcgc accaccggcg ggacgatcgc cacctcacgg
     1021 gtgctgctca cgggaggacc ggcgatgcag gcggtcgcca ccgccgccgg agcccgggcg
     1081 tgggtgggct acgcacgtca ccaggtcgtc gtgaccgagg cgagcccggg actgcacgcc
     1141 gacaccaccg cgatggcgtt cgacatcggc gccggcatct actggcgtcc cgaggagggc
     1201 gggctgctct ggggcatgtc gaaccccgac gaggaaccgg gaccggggcg ttcgatcgac
     1261 tggccgtacc tgcgacggat ggagcgccgg ctccaccgcc tggtgccggt cacgcgcacc
     1321 ctcggcatca agaaggcatg ggcggccacg atcgagtaca ccccggatca tttcccgctc
     1381 tccggtccgc tggtgctgcg cgacggcacg gaggtggccg gcgcctcgat cgccagcgcg
     1441 tgcggccacg ggatgatgtg ggggccggcg gtctcgcgca tcgccgtcga cctcgcgctc
     1501 gccggtgcaa ccgatgtcgt cgaggcggcc gaggccttcc gcatggatcg gttcgacgaa
     1561 cacggcaccc cgccgtccgt cgaccccgtc gccctgccct tcccggtcca cgtggacgac
     1621 gcctgacctc tcgccgcgac gcgtgtcacg gtgcagggag cgtcttcgga cacgtggcga
     1681 tgatgctgcc gtcgccggcg tgtcccaccg tgatcccggt gtcgccgaac gccaccgaat
     1741 cgtcgggtcc cgcgtgccac aggtcgtaga cctcgccgtg acaccgcatc tggtcgccgg
     1801 gtcgcagcac cccaggcgcc cagtagcgcc ggtcccccgc tgccacgtcg aacacccgcg
     1861 cgctcggttc cgtctcgccg gcggcgatca acgcgacggt gatggcggcg agcgagagtc
     1921 cagcggcaac gagcacgatc agggtccggc gtcgcctggc tacgggcttc gcggacgggc
     1981 cggatggtcc ggggtcggac atcgaccgac cctaacgcgc gaggttgtcg gcgtggccga
     2041 agcggcgggt tacagctgga ggacggggca ggtgagggtt cgggtgatgc cttcgagttc
     2101 ctgcatgggc atcacggtgc tcttggcgag ctgatcgagg tcatcggcct ccgcgcgagc
     2161 cacgacgtcg tatgggccgg tgacggggtc cacggccacc actcccggca cgacgcggat
     2221 ctgctcggtg acctgggctg ccttgccgac ctcggtctgg atgaggatgt acgcgttgac
     2281 catggagacc gcctctcgaa ctcgtggggc cttcagctca gctcttgccg ccgccgaaca
     2341 gtcctttgac cttgtcgagc agaccgcccg cggtgtcctt cgccttgccc gccaccgggg
     2401 cggcggcgtc cacggtcttc ccggcgagat cgcccgccgc tcccaccgct ttgccggcga
     2461 cctcgccggc ggcacctacc gccttgccgg ccacgggggc ggcggcgtcc accacaccac
     2521 cggccgtgtc cttggccttg tccacggccg gaccggcctt gcccgccagg tcacccgcgg
     2581 tgcccttggc cttgccggcg agatcaccgg ccgtaccttt cgccttgtcg atgaacccct
     2641 ggtcgtcgct cacgtcggac gttcctctct cctgggccct ccatcgtttc ggtgggggcg
     2701 tgcgaccgca aacctgcagg tcacagggat ggtacgcgtg ttccgggaac cgcgccgacg
     2761 gcgcctgcac gcaggtgcgt gaagttgtgc gtttgatgcc tgtctgcaac acttccgcgc
     2821 atggcgaagg tgaccatcat cggcgcgggc agcgtcgagt tcacccggaa catcctcgcc
     2881 gacctgtgct cgttcaccga gctccacggc acgttgacga tcgccctgca cgacatcgac
     2941 gccgaacggc tcgcgtacgc cgagcgcgca gcgaaccaac tcgtggagcg gttcgacgcc
     3001 gggtaccgcg tcggcgccca cgccgaccgt gcgggtgcgt tcgacggtgc cgactacctc
     3061 atcaacgaga tccaggtggg cggctatcgg gcgaccgtca ccgacttcga agtcccgtca
     3121 cggttcggcg tgcgacagac gatcgccgac acgatcggca tcggcggcat catgcgcggg
     3181 ttgcggacga tcccggtcat ggtggagatg gccgacgaga tcgcgcaacg atcggccgac
     3241 ggctcgctgc tgctcaacta caccaacccc atggcgatgg tgccgtgggg catctgggcg
     3301 gggtcgtcgc tgcccgccga tcgcgtgatc ggcgtgtgcc actcggtccg cgacacgcac
     3361 accttcctcg ccgagaccgt gggcgtgccc gaggccgaca tccgcttcgt caccgccggg
     3421 ttcaaccacc agtgcttcgt ctacacgttc gaggatcgtc gcaccggcaa ggacctgtac
     3481 ccgcgcttgc gcgagatcgt cgaggccgat cccgagggcc tgggacggcg cgtgcgcgtc
     3541 gagctcttcc gccggttcgg ctacttcccc accgagtcca gcgagcattc gagcgaatac
     3601 gtgccgtggt tcctgcacct cgatgaccag gtggcccact tccgatgcga gatcgacgag
     3661 tacatccgcc ggagcgacga gaacctggtg gagttcgccg agaccaagcg ggcgttggac
     3721 gcgggagagg aactcgaggt ggagctcaac gacgagctgg cgtcgcagat cgtgcgggcg
     3781 atcgagaccg gcaccgaacg cgacgtctac accaacatcc gcaacgacgg ccgcatcgac
     3841 ggcctgccca ccgaggcgtg cgtggaggtg ccgacgacgg tgggcgccga gggggtacgg
     3901 cctcatgcgg tcggggcgtt gccggtgcag accctcgcgc tgaaccgcac gttcctcaac
     3961 gtcgtggagc tcaccgtgcg agcggcgctc gagcagcggc gcgacctggt ggaggcggcc
     4021 gccctcctgg atcccaacac cggcgccacg ctgtccaccg cggcgatcca cgagatggtc
     4081 gatgcgttga tcaccgccca cggtgacctg atccccgaag gcatccgccg cgggtgacgg
     4141 gcgccgaggg ccgagtcaga accgcttgaa ccactgggtg agcggatcga cgaagtgacg
     4201 gccgggcacc agcgggtcgc tccacccccc gaggaatacc tcgccacgat cgatgtagcc
     4261 catcgagcgg tagaggccac gtgcctcggc gaaggactcc tccgtcccgg tgtcgagcgc
     4321 gacgccgggc atgcccgcgc cgagcgcctc cgcttcgagc gcttccatca acgcgcggcc
     4381 ggcaccgaga cgccggtgcg aaggcatcac ctcgagatcg ctcaccaagg cgaagccgcg
     4441 ccattcgcac acgtcttcga tccggcgatc
//
LOCUS       DATDBZ010000443         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2808871, whole genome shotgun sequence.
ACCESSION   DATDBZ010000443 DATDBZ010000000
VERSION     DATDBZ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2808871"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VIX62_10530"
     CDS             complement(<1..290)
                     /locus_tag="VIX62_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY5860711.1"
                     /translation="MLVNIHHDNAREHNRLLIAGIAFIVSIALLIWLSIAIYNKEFER
                     VTMVTVKADRAGLQLAKFGDVRVNGALVGQVRSVKQDGKEAAIEVALNPDAAA"
     gene            complement(337..1176)
                     /locus_tag="VIX62_10535"
     CDS             complement(337..1176)
                     /locus_tag="VIX62_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860712.1"
                     /translation="MASPTSIAGSVVQGRRDSLEHYGDQLLFYVKALAWTPRALRRYK
                     REILNTLAEVTFGAGGLTLIAGTVGVIAFLAFFAGTEVGIQGYASLSQIGVAKFTAFI
                     SAYFNTREVAPLVSAIALAATVGCGYTARLGAMRISEEIDALEVMGIPSLPYLVTTRM
                     IAAFVAVIPLYIVALCASYLSPRLITTLIYGQSPGTYDHYFLQFLPPVDMLWSFAKLM
                     FLAVAVILIHCYYGFTASGGPAGVGMAVGRAIRTSIVTVVVADFFLSFAIWGSTTTVR
                     ITG"
     gene            complement(1176..1949)
                     /locus_tag="VIX62_10540"
     CDS             complement(1176..1949)
                     /locus_tag="VIX62_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860713.1"
                     /translation="MGFSAVSVLRGPGEITAMVVEVTKRIFTTPFQFREFLEQAWFIT
                     SVTLMPTILVSIPFGAVISLQVGNLTGQLGAQSFAGATAVLAVTREAAPIAAAMIIAG
                     AAGSAICSDMGARKIREEIDAMEVLGVDPLARLIAPRVVATVFVAVMINGLVIAAGIG
                     GGYFFTVIVQGGSAGAFLSSFTALASLNDLYVAMVKAAVFGWLAAIVGAFKGLNAGGG
                     PSGVGRAVNESVIVAFMLLFFLNAVISAIYFQVAPPPGL"
BASE COUNT          400 a          695 c          758 g          300 t
ORIGIN      
        1 gcggccgcgt cggggttgag cgcgacctcg atcgccgcct ccttgccgtc ctgcttcacg
       61 gagcggacct ggccgacgag cgctccgttg acccgcacgt cgccgaactt ggccagctgg
      121 agcccggcgc ggtcggcctt gacggtcacc atggtgaccc gttcgaactc cttgttgtag
      181 atcgcgatcg acagccagat cagcagggcg atcgacacga tgaacgcgat ccccgcgatc
      241 aggagccggt tgtgctcccg cgcgttgtcg tggtggatgt tcaccagcat cgggaaccca
      301 cctcctccgc gcggctcgcc gaccggacga tcccggtcat ccggtgatcc tcacggtcgt
      361 ggtcgagccc cagatcgcga agctgaggaa gaagtcggcc accacgacgg tgacgatgct
      421 ggtccggatg gcgcgcccca cggccatgcc gacgccggcc ggtccgcccg aggcggtgaa
      481 gccgtagtag cagtggatga ggatcaccgc gaccgcgagg aacatcagct tggcgaacga
      541 ccacagcatg tcgacgggag ggaggaactg gaggaagtag tggtcgtagg ttccgggcga
      601 ctgcccgtag atcagtgtgg tgatcagccg cggggagagg tacgacgcgc agagggccac
      661 gatgtagagc gggatcacgg ccacgaaggc ggcaatcatc cgcgtcgtca ccaggtaggg
      721 aagcgacggg atgcccatca cctcgagcgc atcgatctcc tcggagatgc gcatcgcacc
      781 gagccgcgcg gtgtagccgc aaccgaccgt cgcggcgagc gcgatcgcgg agaccagcgg
      841 agcgacctcg cgggtgttga agtaggccga gatgaacgcg gtgaacttcg cgacaccgat
      901 ctggctcaac gaggcgtaac cctgaatgcc gacctcggtg ccggcgaaga acgcgaggaa
      961 cgcgatcaca ccgaccgtgc cggcgatgag ggtgaggccg ccggcgccga aggtcacctc
     1021 ggcgagcgtg ttgaggatct cgcgcttgta gcggcgcagg gctcgcggcg tccacgcgag
     1081 cgccttgacg tagaacagca gctggtcgcc gtagtgctcg agggagtccc gtcgtccctg
     1141 gacgacggag ccggcgatcg acgtggggct ggccatcaga gccccggggg cggagcgacc
     1201 tggaagtaga tcgcggagat caccgcgttg aggaagaaca ggagcatgaa cgcgacgatc
     1261 acggactcgt tgacggcgcg gcccacccca ctcgggccac cgcccgcgtt gaggcccttg
     1321 aacgccccca cgatggcggc cagccagccg aacacggccg ccttgaccat cgcgacgtac
     1381 aggtcgttca gcgaggccag cgcggtgaag gaggagagga acgccccggc tgatccaccc
     1441 tggacgatga cggtgaagaa gtagccgccg ccgatgccgg ccgcgatcac gaggccgttg
     1501 atcatcacgg ccacgaagac ggtggccacc acccgagggg cgatcaggcg ggcgagcggg
     1561 tcgactccga gcacctccat cgcgtcgatc tcctcgcgga tcttgcgggc acccatgtcc
     1621 gagcagatcg ccgagcctgc ggcgccggcg atgatcatcg cggcggcgat gggggcggcc
     1681 tcccgggtga ccgcgagcac ggccgtggca cccgcgaacg actgcgcgcc cagctggccg
     1741 gtgaggttac cgacctggag ggagatgacc gccccgaacg ggatcgagac caggatggtc
     1801 ggcatcaggg tgacgctggt gatgaaccac gcctgctcga ggaactcccg gaactggaag
     1861 ggggtggtga agatccgctt ggtgacctcg acgaccatcg ccgtgatttc gccgggacct
     1921 cgaaggaccg acacggcgct gaaccccatc ggccacctcc caaccaagtg cgacgagtcg
     1981 ccacacaata gaacgtgttc tcgttttcgt ctacgagacg tgtcttaggg cgtgtcccgg
     2041 ccccatccgg tggctgcctc cgtacagaca acgcgcggag cggctcggag tgacgctcgg
     2101 ggggcgcacg gtaagaggcg cctaccgcgg cgcgtagccg gacggatccc tca
//
LOCUS       DATDBZ010000444         4119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_971300, whole genome shotgun sequence.
ACCESSION   DATDBZ010000444 DATDBZ010000000
VERSION     DATDBZ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971300"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VIX62_10545"
     CDS             <1..254
                     /locus_tag="VIX62_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5860714.1"
                     /translation="DPRRWTDPDRFDVHRKEGAHLAFAIGTHFCLGAWFGRYLSRVSL
                     DLLLDGLPGLRLDPDRPVTLSGWEFRAPDSLWVRWDARS"
     gene            297..704
                     /locus_tag="VIX62_10550"
     CDS             297..704
                     /locus_tag="VIX62_10550"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5860715.1"
                     /translation="MRSIRSNPSSVAPPVGTYSHAAVVEAGEVRWLFVSGQLALDRDG
                     VLVGVGDMAAQTEQVFENLVHILEAHGASFEDVVKIQTFVTTLEGFPEVREVRARYLP
                     EEPPASTAVQVVALVMPDALIEVDLVAVLPTSR"
     gene            726..1223
                     /locus_tag="VIX62_10555"
     CDS             726..1223
                     /locus_tag="VIX62_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860716.1"
                     /translation="MSDEKLIEEAKAYLGGDEQILAAGMFEPRGTTGGMIGATGAGFA
                     TDNIVGEIAGVAAGMESGRAMSHMDGVPRWTMLAVTPTTLYAIKCGQHGVGWQPEETF
                     ATIDRSKAHITVHGRVNVRTLTVEDPGSGRTYQWEGNRIGPAHAKPVIEALEAEEVEP
                     PEGEE"
     gene            1220..2386
                     /locus_tag="VIX62_10560"
     CDS             1220..2386
                     /locus_tag="VIX62_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860717.1"
                     /translation="MTHTPRRPARAETVGSLLRPAALQVAVASFYDEGHSAVLDEERA
                     KDPTALREAEDEAIREVIARQIDSGLDVVTDGEFRRWMFMNSFYDAVSGVRTGKTVSF
                     RNDRGEDIELHIHEIVDRLAPVDAPAAREAAFMRAETDGYPFKVTFPAASIFTHPFTT
                     VAGTDGRGYTSLAEFVDHAIDIERGLVSEAIAAGARYVQFDFPLYPYLVDPAWIARFE
                     AAGHEVDGLLEAAAVADTAVLQGIPDDVTVGLHICRGNFRSSWMCEGSLEPVAERVFG
                     GLPYDSFLVEWDDEGRDGGFESARHLREGSVMVMGIVNSKTPVLEDEDDLIRRMDEAA
                     GFAGGLERLAISPQCGFASVMVGNETDQDAQWRKLELVGRVADRLWGDAERPGR"
     gene            2448..>4119
                     /locus_tag="VIX62_10565"
     CDS             2448..>4119
                     /locus_tag="VIX62_10565"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5860718.1"
                     /translation="MSSFLYRLGQLAARRRWRTVVIWVAAVFAIFAAGSALGGKLADD
                     FNLPDSESQRAYDLLAERYPSASGTSALIVFHSTSGALEEQADAVGSTLDAIAGQPHV
                     VSVTDPLETEGAVSADGSIGYAQVAYDVSAVDLGTEPYTELESAVESGRSPDLEAEIG
                     GEYASWGSQPEPGSSELVGLLAAMIILLIAFGSVVAMGLPIGTAVIGLTTGTGLILIV
                     SAFTSVPEFSTILSSMIGLGVGIDYALFIVTRYRQNLQAGMEPLHAIGLASATAGQAV
                     VFAGITVAIALLGLWISGIAFVGMMATAAAIVVLVAVVAAITLLPALLGFAGNAIDNL
                     SIHRKRKRDETKPAKENMWLRWGHEVERRPWRYFIGAVLVLLLLAVPLFSMKLGFPDD
                     GTAPTSETRRLAYDLLSEGFGPGFNGPLLLAVEIDDPAALQGLEGLNTAIAATEGVAA
                     VTPPQPNEAGDAAIIQVIPTTSPQDQATTDLVRVLRDDTIPGATPEGAVVHVGGATAT
                     FIDLSDRITNARPWFIAAVVLLSFLLLMVVFRSVLVPLKAAVLNLLSIG"
BASE COUNT          622 a         1356 c         1437 g          704 t
ORIGIN      
        1 gtgatcctcg tcgctggacc gatcccgatc ggttcgacgt gcatcgcaag gagggcgcgc
       61 acctcgcctt cgcgatcggc acccacttct gtttgggggc gtggttcggc aggtacctct
      121 cgagggtatc cctggatctg ctgctcgacg ggctgcctgg cctccggctc gaccccgacc
      181 ggccggtgac gctgagcggc tgggagttcc gagcgccgga ctccctctgg gtccggtggg
      241 atgctcgctc gtgaaccagc gcaccacgaa gtacggtgca ccacaggagg tgactgatgc
      301 gatcgatccg atcgaacccc tcatcggtgg cgccgccggt cgggacctac tcccacgccg
      361 cggtggtcga ggccggtgag gtccggtggt tgttcgtctc gggccagctc gccctcgacc
      421 gcgacggtgt gctggtgggg gtcggcgata tggccgcaca gaccgagcag gtcttcgaga
      481 acctggtcca catcctcgag gcgcacggtg cgtcattcga ggacgtcgtc aagatccaga
      541 ccttcgtgac cacgctcgaa ggattccccg aggtgcggga ggtgcgcgct cgctacctgc
      601 cggaggaacc accggcgagc acggcggtgc aggtcgtggc gctggtcatg cctgacgcct
      661 tgatcgaagt cgacctcgtg gccgtcctgc ccacgagccg ttgagtaccg gaaggagccc
      721 gcacgatgag tgacgagaaa ctgatcgagg aagctaaggc ctacctgggc ggcgacgagc
      781 agatcctggc ggcggggatg ttcgaaccgc gtgggaccac cggcggcatg atcggggcta
      841 ccggcgcggg gttcgcgacc gacaacatcg tgggcgagat cgccggtgtg gcggccggca
      901 tggagagcgg ccgcgcgatg tcgcacatgg acggcgtgcc gcgttggacg atgctcgccg
      961 tgacgcccac caccctctac gcgatcaagt gtggccagca cggcgtgggg tggcagcccg
     1021 aggagacgtt cgcgacgatc gatcggtcga aggcgcacat caccgtgcac ggccgggtga
     1081 acgttcgaac tctcacggtc gaggaccccg gcagcggccg gacctaccag tgggagggca
     1141 accggatcgg tccggcccac gccaagcccg tgatcgaggc tctggaagcc gaagaggtgg
     1201 aaccgcccga gggcgaagag tgacccacac accccgccgg cccgcccgcg ccgagacggt
     1261 cggctcgctc ctgcgtcctg ctgcattgca ggttgccgtg gcgtccttct acgacgaggg
     1321 tcactccgcc gtgctggacg aggaacgcgc gaaggatccc accgcgctcc gtgaggcgga
     1381 ggacgaagcg atccgcgagg tgatcgcgcg ccagatcgac agcggcctcg atgtggtgac
     1441 cgacggggag ttccgtcgct ggatgttcat gaactccttc tacgacgcgg tgagcggcgt
     1501 gcggaccggc aagacggtct cgttccgcaa cgatcgtggg gaggacatcg agctccacat
     1561 ccacgagatc gtggatcggc tcgcgcccgt ggatgccccg gctgcgcgcg aagccgcctt
     1621 catgcgggcc gagaccgacg gatatccgtt caaggtgacg ttccccgcgg cctcgatctt
     1681 cacccacccg ttcaccacgg ttgccgggac cgacggccgt gggtacacgt ctctggccga
     1741 gttcgtggac catgcgatcg acatcgaacg aggtctcgtg tccgaggcga tcgctgccgg
     1801 ggcccgatac gtccagttcg acttcccgtt gtacccgtac ctggtcgatc cggcatggat
     1861 cgcacggttc gaggcggcgg gccacgaggt cgacggactc ctggaggctg ccgccgtggc
     1921 cgacaccgcc gtcctccagg ggatccccga cgacgtcacc gtgggcctgc acatctgccg
     1981 tgggaacttc cgctcgtcgt ggatgtgcga gggctcgctc gagccggtcg ccgagcgagt
     2041 gttcggtgga cttccgtacg actcgttcct cgtggaatgg gacgacgagg gtcgtgacgg
     2101 cgggttcgag tcggcccggc acctgcgcga gggttcggtc atggtgatgg gcatcgtcaa
     2161 ctccaagacc ccggtcctgg aggacgaaga cgacctgata cgtcggatgg acgaggccgc
     2221 gggcttcgca ggtggtctgg agcggctggc gatcagcccg cagtgcgggt tcgcgagcgt
     2281 gatggtgggc aacgaaaccg accaggacgc ccaatggcgc aagctggagc tcgtgggccg
     2341 cgtggccgat cgtctgtggg gcgacgccga gcgccccggt cggtgacagt cagcaccgcc
     2401 ttgcctatcg tggaggcgta cgccgtccgc tcctacgaaa ggctcccatg agttcgttcc
     2461 tgtatcggct tggccaactc gctgcgcgtc ggcgctggag aaccgtcgtg atctgggtcg
     2521 ctgccgtgtt cgcgatcttc gccgcaggca gcgcgctcgg tggcaagctc gccgacgact
     2581 tcaacctgcc ggactccgag tcccagcgtg cctacgacct gttggctgag cggtacccat
     2641 ccgcctcggg cacctcggcg ttgatcgtct tccactcgac cagtggggcg ctcgaggaac
     2701 aggccgacgc ggtgggttcc accctggatg ccatcgcggg tcagccccat gtcgtctccg
     2761 tgacggaccc gctcgagacc gagggcgccg tgtcggccga cggctcgatc ggctacgcgc
     2821 aggtggcgta cgacgtgtcc gcagtcgacc tgggaaccga gccgtacacc gagctcgagt
     2881 ccgcggtcga gagtggacgc tcgcctgatc tcgaggccga gatcggcggc gagtacgcgt
     2941 cgtggggatc gcagcccgag cccgggtcct ccgagttggt cggcctgctc gccgccatga
     3001 tcatcctgct gatcgcgttc ggctccgtgg tggccatggg cctacccatc ggcacggccg
     3061 tgatcggcct cacgacggga accgggctca tcctcatcgt gtcggcgttc acgagcgttc
     3121 ctgagttctc cacgatcttg tcctcgatga tcgggttggg tgtcgggatc gactacgcct
     3181 tgttcatcgt cacccgatac cgccagaact tgcaggccgg tatggagccc ctgcatgcga
     3241 tcggcctggc gagcgccacc gcgggccagg cggtcgtgtt cgccgggatc acggtggcga
     3301 tcgcgctgct cgggctgtgg atctcgggga tcgcgttcgt cggcatgatg gccaccgccg
     3361 cggcgatcgt cgtcctcgtg gccgtcgtcg cggccatcac gctgctgccg gccctgctcg
     3421 ggttcgcggg caacgcgatc gacaacctga gcatccaccg caagcgcaag cgcgatgaga
     3481 ccaagccggc gaaggagaac atgtggctgc ggtggggaca cgaggtcgaa cgccgcccgt
     3541 ggcgatactt catcggtgcg gtcctcgtgt tgctgctgct ggcggttccg ctgttctcga
     3601 tgaagctcgg gttccccgat gacggcacgg cgccgacgtc ggagacccgc cgccttgcat
     3661 acgacctgct gtccgagggc ttcggcccgg gcttcaacgg tccgctcctg ctcgcggtag
     3721 agatcgacga tccggcggcg ctgcagggcc tcgaaggcct gaacaccgcg atcgccgcga
     3781 ccgagggtgt ggcggccgtc acgccgcctc agcccaacga ggcgggggac gccgcgatca
     3841 tccaggtcat cccaacgact tcgccacagg atcaagccac caccgatctg gtacgggtgc
     3901 tccgcgacga cacgattccc ggtgccaccc cggagggcgc ggtggtgcac gtcggcggcg
     3961 ccacggccac gttcatcgac ctgtccgacc ggatcacgaa cgcccgaccc tggttcatcg
     4021 ccgccgtggt gctgctgtcc ttcctgctgc tgatggtggt gttccgctcg gtcctggtgc
     4081 ccttgaaggc ggcggtgttg aacctgctgt cgatcggcg
//
LOCUS       DATDBZ010000445         6478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_773028, whole genome shotgun sequence.
ACCESSION   DATDBZ010000445 DATDBZ010000000
VERSION     DATDBZ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773028"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VIX62_10570"
     CDS             <1..1246
                     /locus_tag="VIX62_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5860719.1"
                     /translation="PWRESIADLVTVPEDHDGRIDLDALRETLERHADRPMKIGSFSA
                     ASNVTGILSDVPEISRLLHRHGAISCWDYAAAAPYVQIEMTPRDGDELASLDAIVLSP
                     HKFVGGPGTPGILVARRSMFRNRVPSVPGGGTVAYVNPSEHRYLEDIEHREEGGTPAI
                     VESIRAGLVFQLKEAVGVETIREREDSFTHRAMHRWESNPSIEILGSHDLPRLSIVSF
                     VVRHEARYLHHNFVVAVLNDLFGIQARGGCSCAGPYGHRLLGIDLDTSHAFEREIARG
                     CEGIKPGWVRVNFNYFLTEAEFGFVVDAVDLVATEGWKLLPRYRFDAVTGRWHHRDGQ
                     PEPPMSLRDIRYDDGTPRFESHRHHEADSRLANYIDEARALLSEPASFDPLPDGVVDG
                     TGEDFARLRWFWLPEEVQVTGA"
     gene            1336..2331
                     /locus_tag="VIX62_10575"
     CDS             1336..2331
                     /locus_tag="VIX62_10575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY5860720.1"
                     /translation="MKIEPSNIPREGSIEARLEQAERARFEAEAKYEALLGQLPAAIY
                     AYSPELDGPTFHMSPHVEELLGVPAQEFIDREDIWDVLIHPDDRERSRLDYEEFLRTG
                     QPEHGEYRYVRPDGRVVWVRDHSVMICDPEGKPMFIQGVMTDITPSKEAELRMEHLAF
                     HDALTGLPNRAMFEQHLTLALARARRDGSAVAVVFADLDDFKPVNDTHGHATGDEMLR
                     QVGARLRLAVRETDLVARQGGDEFLVLIADLPPGVEMVDASATVSSIVERIHDVVAQP
                     FPMRVGELVITVSLGCAIFPRDAETTEALLRIADEEMYRRKAENTDRPSPLRRLA"
     gene            complement(2328..2888)
                     /locus_tag="VIX62_10580"
     CDS             complement(2328..2888)
                     /locus_tag="VIX62_10580"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5860721.1"
                     /translation="MTGHESNPWIRRSRATVYENPWIEVLHDEVTRPNGEPGIYGVVH
                     FRSRAVGVVALDADDRVLLVGQYRYTLDRYSWEIPEGGSPLDEDPLEGAQRELAEETG
                     YAARSWRELIRFTLSNSVTDEEGVMFVATDLTEGRASPDPTEALQVRWVSFEDALDMV
                     ASGEIHDVMTQVALLAVAKHRRELND"
     gene            complement(2885..3535)
                     /locus_tag="VIX62_10585"
     CDS             complement(2885..3535)
                     /locus_tag="VIX62_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5860722.1"
                     /translation="MNWIWAVGAPLVRAVIGLLFRVRIEGMEHVPRTGAVIVAPNHVS
                     VIDGPAVSAITGAHRWRATHNLIAGEVFAGLTGWILRQARQIPIRRGTGDTGALDAAT
                     RAIEDGWVVGIFPEGKVSEDPRGPLQRIRSGLTRVAVPTSTPVVPVGIWGTQSMWPKG
                     GLVRAALVQRPRLAMVFGVPLIPTPGEAPTEFRERYRRDLEHQVLRARELAGDPPS"
     gene            complement(3555..4682)
                     /locus_tag="VIX62_10590"
     CDS             complement(3555..4682)
                     /locus_tag="VIX62_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5860723.1"
                     /translation="MARAARGAVVDRRDGSIHVEPLDIDPPQAGEVLIRVAASGVCHS
                     DLWAIENGNWGTTPFPMLLGHEGAGIVEEVGSGVSSVSPGDRVVMSWAIPCGRCRQCL
                     RGMPRRCGHELAQPPRIHRAGTDEGLTGVLCCGTLATHTVVTEPQVIRMPEAIPLSRA
                     CLLGCGVSTGVGAAIQTAAVWPGASVAVIGLGGIGLSALQGSRIAGAARLIAVDVAPA
                     KLEWAVRFGATDLVDASTTDAVEAVRGLTDGGVDIAFEAVGRPECVAQAVAMVGYAGT
                     AVAIGVPPIPAEVTLDWNGTERSAYAQKASLLITDGGDPIPSEDFPQMAAWYLDGSLD
                     IDGMVTRELALTDEDLTEAFRAMLAGEVIRSVVIVDEDAAR"
     gene            4730..5890
                     /locus_tag="VIX62_10595"
     CDS             4730..5890
                     /locus_tag="VIX62_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY5860724.1"
                     /translation="MDVLALRDHAAVRFDDFVATLAQMVNVDCGSYSPAGVNAIADLC
                     EARFRDGGWEVERRPHDPAVGAPRLGDLLIGTLRGSGGPRILLVGHMDTVFDDGTAAA
                     RPFSIDGDIARGPGVSDMKGGLLTGFFAVEVLQAAGFDGFDRITYVCNPDEEIGSPFS
                     GPIIKAMAPEFDAAFVLEGARANGDIVSARKGITDYTIDIVGRAAHAGVEPEKGRNAL
                     LQAAHTIIALQGLNGRWPGVTVNVGVAKGGTRTNVVPERCTLQVDLRSPELETLEAAE
                     AEIARICADTVIPDLQVEVHGDGWHRPMEKKEGGARLAELAIEVAAELGFELRDAATG
                     GASDANTTSAAGTPTIDGLGPIGGDDHAPAEWLDLSSVVPRISLLAGIVARL"
     gene            5887..>6478
                     /locus_tag="VIX62_10600"
     CDS             5887..>6478
                     /locus_tag="VIX62_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5860725.1"
                     /translation="MTNGGVTGEEPFGPEFDVYRALVEGIPAILYIDSLDEWSTNWYT
                     SPQAERLLGFTVEEWGTTPDLWLQRIHPDDRDRVKDENDRSNESREPFTSEYRFITKD
                     GRTVWLRDEALVVTNEGERPFWRGFMHDITAQKEAEEKLRWSLEVLRRTIQQRRELAL
                     RLESAQEEERRRIAADIHDDPIQVMSAVDLRMGMMVE"
BASE COUNT         1077 a         2177 c         2231 g          993 t
ORIGIN      
        1 gccctggcgc gaatcgatcg ccgacctcgt cacggttccc gaagaccacg acgggcggat
       61 cgatctcgac gctctgcggg agacgttgga acgacatgcc gacaggccga tgaagatcgg
      121 gagcttctcg gccgcctcga acgtgacggg catcctcagt gatgtccccg agatctcgag
      181 gttgctgcac cgtcacggag cgatctcgtg ctgggactac gccgctgcgg cgccgtacgt
      241 gcagatcgag atgacgcctc gagacggtga cgaactcgca tcgctggatg cgatcgtcct
      301 gtcgccgcac aagttcgtgg gtggaccggg gacgccgggc atcctggtcg cgcgccggag
      361 catgttccgc aatcgagttc cgagcgtgcc gggtggtggg acggtggcct acgtgaatcc
      421 gtccgagcat cgctacctcg aggacatcga gcaccgtgag gagggcggca cccccgcgat
      481 cgtcgagtcg atccgagcgg ggctggtctt ccaactgaag gaggcggtcg gggtcgagac
      541 gatccgagag cgcgaggact ccttcacgca tcgcgccatg catcgatggg agtcgaaccc
      601 ctcgatcgag atcctgggca gccacgatct gccacggctc tcgatcgtct cgttcgtggt
      661 gcgtcacgag gcccggtacc tgcaccacaa cttcgtggtg gcggtgctga acgacctgtt
      721 cgggatccag gcgcgcggcg gctgctcgtg tgcggggccg tacgggcacc ggctgctggg
      781 gatcgacctg gacacctcac atgcgttcga acgagagatc gcgcgtggat gcgagggcat
      841 caagcccggc tgggtgcgcg tcaacttcaa ctacttcttg acggaagccg agttcggttt
      901 cgtggtcgat gccgtggatc tggtggccac ggaggggtgg aaactgttgc ctcgatatcg
      961 cttcgacgcc gtgaccggcc ggtggcatca ccgtgacggc cagcccgagc ccccgatgag
     1021 cctgcgagac atccgctacg acgatggcac gcctcggttc gagagccatc gccaccacga
     1081 ggccgactcg aggctggcga actacatcga cgaggcgcgt gccctgctgt cggaaccagc
     1141 atcgttcgat ccgcttcccg acggggtcgt ggatggaacc ggtgaggact tcgcgcgcct
     1201 tcgatggttc tggctcccgg aagaggtgca ggtgacagga gcctgaacca gggacggttc
     1261 ggtgatcctt cagcgggcag cgatccggcc gataaccttg tgagcacgtc tcgctcccgc
     1321 gacaggacga caccgatgaa gatcgaaccc tcgaacatcc cgagggaggg ctcgatcgag
     1381 gcacgcctgg agcaggccga acgcgctcgc ttcgaagcgg aggccaagta cgaggccctg
     1441 ctcgggcaac tcccggcagc gatctatgcc tactccccgg agctcgacgg accgacgttc
     1501 catatgagtc cgcacgtgga ggagctgctc ggtgtcccgg cgcaggagtt catcgatcgt
     1561 gaggacatct gggatgtgct gatccacccc gacgaccgcg agcggtcacg cttggactac
     1621 gaggagttcc tgcggaccgg tcagccggag cacggcgagt atcggtacgt gcgtcccgac
     1681 ggtcgcgtcg tctgggttcg cgaccactcc gtgatgatct gcgacccgga gggcaagccg
     1741 atgttcatcc agggtgtgat gaccgacatc accccgagca aggaagccga gctccgcatg
     1801 gagcacctcg cgttccacga cgccctcacc ggtttgccga atcgagccat gttcgaacag
     1861 cacctcacct tggccctggc tcgtgcgcgc cgcgacggtt ccgccgtggc ggtcgtcttc
     1921 gccgacctcg atgacttcaa gccggtgaac gacacgcatg gccatgccac gggcgacgag
     1981 atgctgcggc aggtcggcgc acggttgcga ttggccgtga gggagaccga tctggtggca
     2041 agacagggag gcgatgagtt cctggtgctg atcgccgacc tcccgcccgg tgtcgagatg
     2101 gtcgacgcga gcgccaccgt ctcctcgatc gtggaacgga tccatgatgt ggtcgcgcag
     2161 ccgttcccga tgcgggtggg ggagctcgtg atcacggtga gtctgggatg cgcgatcttc
     2221 ccccgcgacg ccgagaccac cgaagccctc ctgcggatcg ccgacgagga gatgtatcgg
     2281 cgcaaggccg agaacaccga tcgcccgagc ccgctgcgca ggttggccta gtcgttcagt
     2341 tcgcgtctgt gcttcgccac ggcgaggagc gccacctgcg tcatcacgtc gtggatctcg
     2401 cccgaggcca ccatgtcgag cgcgtcttcg aacgacaccc agcgaacctg gagcgcctcc
     2461 gtcggatcgg gcgaggctcg accttccgtc agatcggtgg cgacgaacat cacgccttcc
     2521 tcgtcggtga ccgagttcga cagggtgaat cggatcagct cgcgccagga gcgggcggcg
     2581 tacccggttt cctcggcgag ctcgcgttgg gctccttcga gcggatcctc atcgagcggg
     2641 ctcccgccct ccgggatctc ccaggagtag cgatccagcg tgtaccgata ctggcccacc
     2701 aacaggacac ggtcgtcggc atcgagcgcg accacgccga cggcgcggct ccggaaatgg
     2761 accacgccgt agatccccgg ttcaccgttc ggccgggtca cctcgtcgtg cagcacctcg
     2821 atccacgggt tctcgtagac cgtggcacgc gagcggcgga tccacgggtt ggactcgtgg
     2881 ccggtcacga cgggggatct ccggcgagct cgcgagcgcg caggacctgg tgttccaagt
     2941 cacggcgata ccgttcgcgg aactcggtgg gcgcttcgcc gggcgtgggg atcaagggga
     3001 cgccgaagac catcgccagg cggggccgtt ggaccaacgc agcgcggacc aatccgccct
     3061 tcggccacat cgactgcgtg ccccagatcc cgaccggcac gactggggtc gaggtcggca
     3121 cggcgacccg cgtgaggccg gagcggatgc gctgcagcgg gccacgagga tcttcactga
     3181 ccttgccctc ggggaagatc ccgaccaccc atccgtcctc gatcgcccgg gtcgcggcgt
     3241 ccagggcccc cgtgtcaccg gtgccccgac ggatcgggat ctgccgggcc tgccgcagga
     3301 tccagcccgt gagccctgcg aacacctcgc cggcgatgag gttgtgggtg gcgcgccagc
     3361 gatgcgcgcc ggtgatcgcc gagaccgcag ggccgtcgat cacgctgacg tggttcgggg
     3421 ccacgatcac cgcgccggtc ctgggcacgt gttccatgcc ctcgatccgg acacggaaca
     3481 acaggccgat gacggcgcga accagtgggg cacccaccgc ccagatccag ttcatctcgc
     3541 gcacgtcgcg gatgctaccg ggccgcgtcc tcgtccacga tgacgaccga gcggatcacc
     3601 tcgcccgcca gcatggcccg gaaggcctcg gtgaggtctt catcggtgag cgcgagctcg
     3661 cgcgtcacca tcccgtcgat gtccagcgag ccgtccaggt accaggccgc catctgcggg
     3721 aagtcctccg aggggatggg gtcgccgccg tccgtgatca gcaacgacgc cttctgcgcg
     3781 taggccgagc gctcggtgcc gttccagtcg agcgtgacct ccgccgggat cggcggcaca
     3841 ccgatcgcca ccgccgtgcc ggcatagccc accatcgcga ccgcctgagc cacgcactcg
     3901 ggccggccca ccgcttcgaa cgcgatgtcg acaccgccgt cggtcaagcc acggaccgcc
     3961 tcgaccgcat cggtggtgga ggcatcgacg aggtcggtgg caccgaaccg gacagcccac
     4021 tccagcttcg ccggcgcgac gtccaccgcg atcagcctcg ccgcgccggc gatcctcgag
     4081 ccctgcagcg ccgacagacc gatgccgccg aggccgatga ccgcgacact ggcgccgggc
     4141 cacaccgcgg cggtctggat cgcggcgccc accccggtgg acaccccgca tccgagtaga
     4201 caagctcgcg acaaggggat cgcctcaggc atccggatca cctggggttc cgtgaccacc
     4261 gtatgggtgg cgagcgtgcc gcagcacagg accccggtga gcccttcgtc ggtgccggcg
     4321 cgatggatcc gcggaggctg ggccagctcg tgcccgcacc ggcggggcat gccgcgcaga
     4381 cactggcggc agcggccgca ggggatggcc cacgacatga cgacgcggtc gccgggcgac
     4441 accgacgata cgccggatcc gacctcctcc acgatgccgg ctccttcgtg gccgagcagc
     4501 atcgggaacg gcgtggttcc ccagttgccg ttctcgatcg cccacaggtc ggagtggcac
     4561 accccgctgg ccgcgacacg gatcagcacc tccccggcct gtggtgggtc gatgtcgagc
     4621 ggttcgacgt ggatcgatcc atcccgtcgg tcgacgacgg ctcctcgtgc tgctcgtgcc
     4681 atccacgtcc tcctgtgtgc cgcgaggtgg tcatgctagc gtcagccgca tggacgtcct
     4741 tgcgctgcgc gaccatgccg ccgtgcgctt cgatgacttc gtcgccacct tggcgcagat
     4801 ggtgaacgtg gactgcggct cgtattcgcc cgcgggcgtc aatgcgatcg ccgacctgtg
     4861 cgaggcgcgg ttccgcgacg gcggatggga ggtggagcgc cggccccatg accccgcagt
     4921 cggtgcacca cgcctcggcg acctcctgat cggcaccctc cgcggctccg gcggacctcg
     4981 gatcctgttg gtgggacaca tggacaccgt gttcgacgac ggcaccgcag cggcgcgacc
     5041 gttctcgatc gacggagaca tcgcgcgcgg gccgggggtg tccgacatga agggcggcct
     5101 gctgaccggc ttcttcgcgg tggaggtgct gcaggcggcc gggttcgacg gattcgaccg
     5161 catcacctac gtctgcaacc ccgacgagga gatcggatcg ccgttcagcg ggccgatcat
     5221 caaggcgatg gcgccggagt tcgacgcggc gttcgtcttg gagggggccc gggcgaacgg
     5281 cgacatcgtg agcgcgcgca agggcatcac cgactacacg atcgacatcg tcgggagggc
     5341 ggcccacgcc ggcgtggaac cggagaaggg gcgcaacgcg ctgctccagg ccgcccacac
     5401 gatcatcgcg ctgcagggtc tcaacggacg ttggcccggc gtcacggtga acgtgggggt
     5461 cgccaagggc ggcacgcgca ccaacgtggt gccggaacgg tgcacgctgc aggtcgatct
     5521 ccggtcgccg gagctggaaa ccttggaggc ggccgaagcc gagatcgcgc gcatctgcgc
     5581 cgacacggtg atccccgatc tgcaggtcga ggtgcacggc gacggctggc accggcccat
     5641 ggagaagaag gaaggcggcg cccggctcgc tgagctcgcg atcgaggtcg cggccgagct
     5701 cgggttcgag ctccgggacg ccgccaccgg tggcgcatcc gatgccaaca cgacgagcgc
     5761 ggccggcacc ccgacgatcg atggactggg cccgatcggc ggcgacgatc acgcgcccgc
     5821 ggaatggctc gacctgtcca gcgtggttcc gcggatctcc ctgctggcag ggatcgtggc
     5881 tcggctatga cgaacggcgg cgtgaccggc gaggaaccgt tcggcccgga gttcgacgtc
     5941 taccgcgcgc tggtcgaagg catcccggcg atcctctaca tcgattcgct cgatgaatgg
     6001 tcgaccaact ggtacacgag cccgcaagcc gagcgactcc tggggttcac ggtcgaagaa
     6061 tgggggacga cgcccgacct gtggctgcag cgtatccacc cggacgaccg cgaccgggtc
     6121 aaggacgaga acgaccggtc gaacgaatcc cgtgaaccgt tcacgtccga gtaccgattc
     6181 atcacgaagg acggacgcac ggtgtggctg cgcgacgagg cgctggtcgt caccaacgaa
     6241 ggcgagcggc cgttctggcg tggattcatg cacgacatca cggctcagaa ggaagcggag
     6301 gagaagctcc gatggagcct cgaggtgctc cggcgcacca tccagcagcg gcgcgaactt
     6361 gcgctgcgcc tggagagcgc gcaggaggag gagcgtcgcc ggatcgccgc cgacatccac
     6421 gacgacccga tccaggtgat gagcgccgtc gacctccgca tgggcatgat ggtggagc
//
LOCUS       DATDBZ010000446         2369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_972649, whole genome shotgun sequence.
ACCESSION   DATDBZ010000446 DATDBZ010000000
VERSION     DATDBZ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972649"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VIX62_10605"
     CDS             complement(<1..704)
                     /locus_tag="VIX62_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5860726.1"
                     /translation="MTGDRPVALVLGASADAPPPGLEAAADVLELRYAPDADSLAAAL
                     PRAQALFFWHGEREELESAWPAAGDLRWIQTASAGVDGLLFPDLVDSDVVVTNARGVF
                     DDPIAEWVIGAMLAFATGLHRSIVDQQAGVWGKDRSTERLEGKHLTVVGPGPIGRATA
                     SRALALGMIVTLVGRSARHDEAFGTVVGPEGFAVALAAADYVLDALPFTPDNAELFDA
                     AAFAAMRPSARFINVGR"
     gene            complement(701..1069)
                     /locus_tag="VIX62_10610"
     CDS             complement(701..1069)
                     /locus_tag="VIX62_10610"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY5860727.1"
                     /translation="MTERRFTVEEADAELADLRERLPRLRQARRDLIAASERITERVA
                     ADGGGVSEAGWFTYQQTLKDDLASLADRGILLRDPEMGLVDFPTERDGERVMLCWKLG
                     EERVAHFHTERAGMSGRRPL"
     gene            complement(1066..1770)
                     /locus_tag="VIX62_10615"
     CDS             complement(1066..1770)
                     /locus_tag="VIX62_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5860728.1"
                     /translation="MTIRAVLFDVGETLVHPAPSFPALFSQVLDREGHTRDEADVLAA
                     SAVVLERFSEASRERVAWTLSPEASRAFWIDVYQRMLVSLELPSANGLRETLYAAFTD
                     LENYALFDDVHPVLASLREQGITAGIVSNFEAWLDQLLVDLEVRDEFPVRVISGLEGM
                     EKPDLEIYALALERLGLPAEQVAFVGDNPEFDIVPPMALGMTAVLIDRRERHADHDGI
                     RITDLRDLPRSLEEAS"
     gene            complement(1767..>2369)
                     /gene="coaE"
                     /locus_tag="VIX62_10620"
     CDS             complement(1767..>2369)
                     /gene="coaE"
                     /locus_tag="VIX62_10620"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY5860729.1"
                     /translation="PPAPRDMRFIGLTGGIGSGKSTVARMLADRGAVILDSDVLAREV
                     VEPGTPGFEAVVARFGPEIVTPEGGVDRPALARVVFADDDARRDLEAIVHPLVRRRVA
                     EEVAARVGTNDVVVLDSPLLIETGTYRDCQQVLVVSALPETQIRRLVARGMDEADARA
                     RLAAQMPLEDKVVFADVVFDNEGTLQELEAQVDRFWRSLS"
BASE COUNT          384 a          867 c          786 g          332 t
ORIGIN      
        1 cgacccacgt tgatgaaccg ggcggacggt cgcatcgccg cgaaagcggc cgcatcgaac
       61 aactcagcgt tgtccggcgt gaacggcagg gcgtcgagca cgtagtcggc ggcggcgagc
      121 gccacggcga acccctcggg cccgaccacc gtgccgaacg cctcgtcgtg gcgggcgctt
      181 ctgcccacca gggtgacgat catccccagg gcgagtgcgc ggctggccgt ggcgcggccg
      241 atcggacccg gccccacgac ggtgaggtgc ttgccctcga gccgctccgt acttcgatcc
      301 ttgccccaga cgcctgcctg ctgatcgacg atcgaacggt gcaggcccgt cgcgaacgcc
      361 agcatggcgc cgatcaccca ttcggcgatc ggatcgtcga acacgccgcg cgcgttcgtg
      421 accacgacgt cgctgtcgac gaggtcgggg aacagcagtc cgtcgacccc cgccgaggcg
      481 gtctggatcc atcgcaggtc gccagcggcc ggccacgcgc tctcgagctc ctcccgttcg
      541 ccatgccaga agaacaacgc ctgcgcacgg ggcagcgcgg cggccagcga gtcggcatcg
      601 ggcgcgtagc ggagttccag gacgtcggcg gccgcctcga ggccgggcgg cggggcatcg
      661 gcggacgctc ccagcacgag ggctacggga cggtcgccgg tcacagcggc cgccttccgc
      721 tcatcccggc gcgctcggtg tggaagtggg cgacgcgttc ctcccccagc ttccagcaca
      781 acatcacgcg ctcaccgtcg cgttcggtcg ggaaatcgac caggcccatc tccggatccc
      841 gcagcaggat gccccggtcg gcgagggagg ccagatcgtc cttcaacgtc tgctgatacg
      901 tgaaccagcc ggcctccgac acccctccgc cgtcggcggc gacccgctcg gtgatccgtt
      961 cgctcgcggc gatcagatcc cggcgagctt gccgcaaccg gggtagccgt tcccggagat
     1021 cggccaattc ggcatcggcc tcctccaccg tgaaacgccg ctcggtcacg acgcctcctc
     1081 gaggctccgg ggaagatcgc ggaggtcggt gatgcggatc ccgtcgtgat cggcgtggcg
     1141 ctcacggcgg tcgatcagca cggcggtcat ccccagcgcc atcggtggaa cgatgtcgaa
     1201 ctcggggttg tcccccacga acgccacctg ctccgccggc agcccgagac gctccagcgc
     1261 caaggcatag atctcgaggt cgggcttctc catcccttcc agcccgctga tcacccggac
     1321 cgggaactcg tcccgcacct ccagatccac cagcagctgg tccaaccacg cttcgaagtt
     1381 cgacacgatc cccgcggtga tcccctgctc gcggagggat gccagcacag ggtggacgtc
     1441 gtcgaacagc gcgtagttct ccagatcggt gaacgccgcg tacagggtct cgcggaggcc
     1501 gttcgccgac ggcagctcga gggagaccag catgcgctga tagacgtcga tccagaacgc
     1561 gcgagaggcc tccgggctca gggtccaggc cacgcgttca cgggaggctt cgctgaaccg
     1621 ttcgagcacg accgccgacg ctgccagcac gtcggcttcg tcgcgggtat gaccctcgcg
     1681 atcgagcacc tgggagaaca acgcggggaa cgagggcgcc ggatggacca gggtctcgcc
     1741 gacgtcgaag agcacggcgc ggatcgtcac gacagcgacc tccagaaccg gtcgacctgc
     1801 gcctcgagtt cttggagcgt cccctcgttg tcgaacacca cgtcggcgaa caccaccttg
     1861 tcctccagcg gcatctgggc cgccaatcgg gcgcgggcgt ccgcctcgtc catgccgcgc
     1921 gccacgagcc ggcggatctg cgtctcgggc agcgccgaga ccaccagcac ctgctggcag
     1981 tcgcggtagg tcccggtttc gatcagcagc ggcgagtcga gcaccaccac gtcgttggtg
     2041 cccacacggg ccgccacttc ctcggccacg cgacgccgga ccaacgggtg gacgatcgcc
     2101 tcgaggtccc tgcgggcgtc gtcgtcggca aacaccacac gggcgagcgc cgggcggtcc
     2161 acgccacctt cgggcgtcac gatctcggga ccgaaccgcg ccaccacggc ctcgaagccg
     2221 ggcgttcccg gttcgaccac ctcacgggcg agcacgtcgg agtccaggat cacggcacca
     2281 cggtcggcga gcatgcgagc cacggtggac tttcccgaac cgatcccgcc ggtgagcccg
     2341 atgaaccgca tgtctcgtgg agccggggg
//
LOCUS       DATDBZ010000447         2351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1790002, whole genome shotgun sequence.
ACCESSION   DATDBZ010000447 DATDBZ010000000
VERSION     DATDBZ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790002"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1391)
                     /locus_tag="VIX62_10625"
     CDS             complement(162..1391)
                     /locus_tag="VIX62_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY5860730.1"
                     /translation="MEGHGPDLIATIAIGLGAAFVGGFLARLVKLPSIVGYLLAGVAV
                     GPFTPGFVADQGIALELAEIGVALLMFGVGLHFSVRDLLRVRQVAVPGALGQIAISAA
                     LGVGLGVAFGWDLVSGLVLGLAVSVASTVVLLRALRQRDELDTDAGRIATGWLIVEDL
                     FTVIALVLLPILAVAAGTVEGAEPLSAGGIMLEIGSALGKAAILTAIMLIVGARALPW
                     LLTHVQGQGSRELFTLAVVAVALGVAYAAAQIFDVSLALGAFLAGAVLSESRVSERAA
                     HDVLPLTDVFSVLFFVSVGMLLDPGILRDEPLAIAAVVGVVIVGKGLAALLLVAALKR
                     PLSTGLVVAAGLAQIGEFSFIVATTSLTLDLMPAKGFQLVVAASIISITLNPALFAMI
                     RPVEGWAARKRAAAGVQ"
     gene            1499..1876
                     /locus_tag="VIX62_10630"
     CDS             1499..1876
                     /locus_tag="VIX62_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5860731.1"
                     /translation="MVFRIEIVPGGPPASAQAREALRDLIDRGEILPGERLPTVRALA
                     ADAALAPNTVARAYRELEEAGYLEGRGRAGTFVVEEPPPVSGDAEAALADAADRYLVR
                     AKQLGFDASAATHALDVATRRTR"
     gene            complement(1883..2296)
                     /locus_tag="VIX62_10635"
     CDS             complement(1883..2296)
                     /locus_tag="VIX62_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5860732.1"
                     /translation="MTIDAERLRQLDLFVDLDSYDLSQVCRWVHEVRADAGDALFEQG
                     AIPYEMFVIEQGEVEVVRDGEVLAKLGPGETVGEMGLMMQERRMASVRAATPLVALAI
                     PADDLAELQAEMPEVWASIRATMEERRRRNVLDED"
BASE COUNT          388 a          847 c          809 g          307 t
ORIGIN      
        1 acgcacggac cgatcggcgt cgtggaccgc gtgcatgagg tcggcggtga aggtggccac
       61 gccgcaacgc cggggtggat aggtcgaaac gaaggcgacg cgcatccggt cactgtatcc
      121 gacgcgtccc ggagcctcgg tcccgccatc gtgtcgatgg actactgaac gcctgctgcc
      181 gcacgcttcc gtgcggccca gccctcgacg ggccggatca tggcgaacag cgccgggttc
      241 agcgtgatcg agatgatcga ggccgccacc accagctgga agcccttcgc gggcatgagg
      301 tcgagggtca gcgacgtcgt cgccacgatg aacgagaact cgccgatctg cgccaacccg
      361 gccgccacga ccaagcccgt cgagagcgga cgcttgaggg cggccaccag caggagcgcc
      421 gccagccctt tgccgacgat caccacgcct accacggcgg cgatcgccaa tggttcgtcg
      481 cgcaggatcc cggggtcgag cagcatcccc accgacacga agaagagcac gctgaacacg
      541 tcggtgagcg gcaacacgtc gtgggcggcc cgttcgctca cccgtgactc gctgagcacc
      601 gcccccgcga ggaaggcgcc gagggccagc gagacgtcga agatctgggc ggccgcgtag
      661 gccaccccca gcgcgacggc caccacggcc agcgtgaaca gttccctgga tccctgcccc
      721 tggacgtggg tgagcagcca cggcagggct cgcgcgccga cgatgagcat gatcgcggtg
      781 aggatcgcgg ccttgccgag cgcggagccg atctccaaca tgatcccgcc cgccgacagt
      841 ggttcggcgc cttccacggt gccggccgcc acggcgagga tgggcaggag gacgagggcg
      901 atcaccgtga acaggtcctc gacgatcaac cacccggtgg cgatgcgccc ggcgtcggtg
      961 tcgagttcgt cccgctgccg caacgcgcgc agcaggacca cggtgctcgc caccgacacg
     1021 gcgaggccca ggaccaggcc cgacaccagg tcccagccga acgccacgcc caatcccacg
     1081 ccgagcgcgg cggagatcgc gatctgtccc agcgcaccgg gcaccgccac ctgccgaacc
     1141 cgcagcaggt cgcggacgct gaaatgcaga cccacgccga acatcagcag tgcgacgccg
     1201 atctccgcga gttcgagcgc gatcccctgg tcggcgacga agccgggggt gaacgggccc
     1261 accgcgaccc ccgccaggag gtagcccacg atcgacggga gcttcacgag gcgtgcgagg
     1321 aacccaccga cgaaggccgc gcccagtccg atcgcgatcg tggcgatcag gtcggggccg
     1381 tggccttcca tggatggtcc tccgcatcgc ttcttggccg ggtcgcgggc ctcgccgtgc
     1441 cccacgactc taatgaacga gccgggaagg ccgcttcggc tgtgatatca ccgcgcgcat
     1501 ggtgttcagg atcgagatcg tgcccggtgg cccgcctgcg tcggcgcagg cgcgcgaggc
     1561 gttgcgtgac ctgatcgacc gcggcgagat cctgcccggt gaacggctcc ccacggtccg
     1621 ggcgctcgcc gcggacgcgg cgctggcgcc caacaccgtc gcccgggcct accgcgaact
     1681 cgaggaggcg ggctacctcg agggccgggg gcgtgccgga acgttcgtgg tggaggagcc
     1741 gccgccggtg agcggggacg cggaggcggc gctcgccgat gccgccgacc gctacctggt
     1801 tcgtgcgaag cagctcgggt tcgatgcctc ggcggcgacc cacgctctgg acgttgctac
     1861 gcgccgaacg cgctgaccga ggtcagtcct cgtcgaggac gttgcgccgg cgacgctctt
     1921 ccatcgtggc ccggatcgag gcccacacct cgggcatctc ggcctgcagc tcggcgagat
     1981 cgtcggccgg gatcgcgagc gcgaccagcg gcgtggcggc gcggaccgag gccatgcgcc
     2041 gttcctgcat catcaggccc atctccccga cggtctcgcc gggtccgagc ttggccagga
     2101 cctccccgtc gcggaccacc tcgacctcgc cctgctcgat cacgaacatc tcgtagggga
     2161 tcgcgccctg ttcgaacaac gcgtcgcccg cgtcggcgcg gacctcgtgc acccagcggc
     2221 acacctgcga caggtcgtag gaatccaggt cgacgaacag atccagctgg cgaagtcgtt
     2281 ccgcgtcgat cgtcatcggt catggctccc ggtcaccgcg acagtatgcg ccatgggcga
     2341 tcaggggtgc c
//
LOCUS       DATDBZ010000448         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1368569, whole genome shotgun sequence.
ACCESSION   DATDBZ010000448 DATDBZ010000000
VERSION     DATDBZ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368569"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VIX62_10640"
     CDS             <1..950
                     /locus_tag="VIX62_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5860733.1"
                     /translation="NRLIEPTGGRIVLDGTNVLDQDPVSLRRGIGYVIQHTGLLPHRS
                     IRQNIATVPHLVGWDDARIGARVDELLEIFDLDAELLDRYPAELSGGQRQRVGVARAL
                     AADPPVMLMDEPFAAVDPIVRSRLQDQFLEIQERLRKTIVFVTHDVDEAIKLSDRMAI
                     LNVGGVLEQFAPPETVLREPANDFVREFVGAERGLKRLALIKVGDIEVEDGPVVTPGV
                     SADDARRAIDAADFGWVSVVDEGELLGWVDHDALEGCTTAGEAAARPFSAYVTARDSL
                     RQALDSIVTSRTAVAVVVTEGQHYRGILTLERISKEIVA"
     gene            947..1657
                     /locus_tag="VIX62_10645"
     CDS             947..1657
                     /locus_tag="VIX62_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018469979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860734.1"
                     /translation="MILLEAMAGLFAQEDTEPLIRWIWITDHVDEIVSRGLQHVYLTV
                     VTVVIGFLIAFPAAVFASRHRWAVSPITSVSGVLYTVPAFAFIFLLLPITGLSLTTVV
                     IPLIAYSLLILFRNTLAGIESVEPEVKEAATGMGLSRRQLLWRVEVPLAMPVIIAGLR
                     IATVSSIALITIAALIGRGGFGQFILEGLNTFFWTPLIVGVVLSVVLAFLADVLLLGL
                     QRLMTPWARAAGVRAVAT"
     gene            1654..>2104
                     /locus_tag="VIX62_10650"
     CDS             1654..>2104
                     /locus_tag="VIX62_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5860735.1"
                     /translation="MNGLLEGLQWLADPANWSGVDGVPARVWEHVQLSLISLAIATLL
                     AVPLGLWIGHTRRGQFWTVQLANVGRSVPSLAVLSIMFLIAVKEFPSLAFGFLPTIVA
                     LTLLGIPVILINTYVGIQQVDRDTVEAARGMGMDGRQVLWRLEVPLAM"
BASE COUNT          283 a          695 c          763 g          363 t
ORIGIN      
        1 tcaaccgcct gatcgagccg accggcggcc gcatcgtcct cgacggcacg aacgtgctcg
       61 accaggaccc ggtgagcctg cgacgcggca tcgggtacgt gatccagcac acgggcctgc
      121 tgccgcaccg ctcgatccgc cagaacatcg cgaccgtgcc gcacctggtg gggtgggacg
      181 acgcccgcat cggcgcccgc gtcgacgagt tgctggagat cttcgacctc gatgcggagc
      241 tgctcgatcg ctacccggcg gagctctcgg ggggtcagcg gcagcgggtg ggtgtcgccc
      301 gggcgctcgc ggccgatccg cccgtgatgc tgatggacga gcccttcgcg gcggtcgacc
      361 cgatcgtgcg gtcgcggctg caggaccagt tcctcgagat ccaggaacgc ctgcgcaaga
      421 cgatcgtctt cgtgacccac gacgtcgacg aggcgatcaa gctcagcgac cgcatggcga
      481 tcctgaacgt cggcggtgtg ctcgagcagt tcgcgccgcc cgagacggtg ctgcgcgagc
      541 ccgcgaacga cttcgtccgg gagttcgtgg gcgccgaacg cgggctgaag cggctcgcgc
      601 tgatcaaggt cggcgacatc gaggtcgagg acgggccggt cgtcacgccg ggcgtgtcgg
      661 ccgacgacgc tcgccgggcg atcgatgcgg cggacttcgg gtgggtgagc gtggtggacg
      721 agggcgagtt gctcgggtgg gtcgaccacg acgcgctcga agggtgcacg accgcgggcg
      781 aggcggccgc ccgtccgttc agcgcctacg tgaccgcccg tgactcgctg cggcaggcgc
      841 tcgactcgat cgtgacgtca cgaacggcgg tggccgtcgt cgtgaccgag ggacagcact
      901 accgaggcat cctgacgctg gaacgcatct cgaaggagat cgtggcgtga tcctgcttga
      961 ggccatggcc gggctgttcg cgcaggagga caccgagccg ctgatccgct ggatctggat
     1021 caccgaccac gtcgacgaga tcgtgagccg ggggctccag cacgtctacc tcacggtcgt
     1081 gacggtggtg atcggcttct tgatcgcgtt ccccgcggcc gtgttcgcgt cgcggcaccg
     1141 atgggcggtc tcgccgatca cgtccgtctc gggcgtgctc tacacggtgc cggcgttcgc
     1201 gttcatcttc ctcctgctgc cgatcacggg cctgtcgctc accacggtcg tgatcccgct
     1261 gatcgcctac agcctgttga tcctgttccg caacacgctc gcgggcatcg agtcggtgga
     1321 gcctgaggtg aaggaggccg ccaccggcat ggggctgtcg cgccgccaac tgttgtggcg
     1381 cgtggaggtc ccgctcgcga tgcccgtgat catcgccggc ctgcggatcg cgacggtgag
     1441 ctcgatcgcc ctgatcacga tcgcggcgct gatcgggcgg gggggcttcg ggcagttcat
     1501 cctcgagggg ctgaacacgt tcttctggac acccctgatc gtcggcgtcg tgctctcggt
     1561 cgtcctcgcc ttcctggcgg acgtcctctt gctggggctg cagcgcctga tgaccccgtg
     1621 ggcgagggcc gcgggtgtgc gcgcggtggc gacgtgaacg gcctcctcga gggcctgcag
     1681 tggctcgccg atccggcgaa ctggtcgggc gtcgacgggg tgccggccag ggtctgggaa
     1741 cacgtgcagc tctcgctgat ctcgctcgcg atcgcgacgc tgctcgcggt gccgctcggc
     1801 ctgtggatcg ggcacacgag gcgagggcaa ttctggaccg tccagctggc gaacgtcggg
     1861 cggtcggtcc cgtcgctcgc ggtgctgtcg atcatgttcc tgatcgccgt gaaggagttc
     1921 ccctcgctgg cgttcgggtt cctgcccacg atcgtggccc tcacgctgct cgggatcccg
     1981 gtgatcctga tcaacaccta cgtcggcatc cagcaggtcg atcgcgacac ggtcgaggcg
     2041 gcccggggca tgggcatgga cggcagacag gtcctgtggc ggctcgaggt gccgctcgcg
     2101 atgc
//
LOCUS       DATDBZ010000449         2551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_350123, whole genome shotgun sequence.
ACCESSION   DATDBZ010000449 DATDBZ010000000
VERSION     DATDBZ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350123"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VIX62_10655"
     CDS             complement(<1..624)
                     /locus_tag="VIX62_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEY5860736.1"
                     /translation="MRSRGLVVAIAVVLAIVAAAAVILYTNGVKNDAVTGGALSVVVV
                     SKQDIAANTNLNPLLEEGAFTELRVPTEAVVDGAVTSMSELRGQTTTAPILANEQIAS
                     PRLSSGEAPTGGPLGITPGNVGLPMVLEGGPAGYGVIQRGDSVAVYASFKAGEIVPKA
                     ALRQILSPAQLSKLLSETSTNGPLVAIPFKFTVTVVQATRVLNIQNPV"
     gene            complement(650..2074)
                     /locus_tag="VIX62_10660"
     CDS             complement(650..2074)
                     /locus_tag="VIX62_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY5860737.1"
                     /translation="MRAGLIARIRDARQRSGEESGAVVVIVVLSLIAIFAMLVLTVDV
                     GGLLLARRAMVNASDSAALAAAQSCASAEDTLDPESVADTYAADNVDGVITGTSNITE
                     IVGCDDGNGHVSVEYLTQQNLFFAGVLGFGSTGDVSTVATAAWGPAAGGYAVPIVLES
                     GYLQGICKVPDGVSIGDTCAMYYNNGDASLGEANWGFMNLDMWNVDAGENCHSAGSSD
                     RADWILNDYGDPLVLNGTPPGSQPTYVCNDTGHSTSNWQNLVDRMEANAVVLFPVNDC
                     EGQLDKSGSVSPCPSTPDKYDIIGFTRLKLIEVYEGDDPAAIGTTGASGTCTLSKASL
                     TNGQVKPLSDTYGTGGGCPTGTPDNLNAADVHVYPKKGPEYQQCTPGDISATCGYWYD
                     PVARQITWRQPNETDLKVEYGWSMNGTEGACGTRRSDPNAICIVTEWRGFTTSSGAIG
                     SGQNFGVSAFALCDLELGTCPEES"
     gene            complement(2059..2511)
                     /locus_tag="VIX62_10665"
     CDS             complement(2059..2511)
                     /locus_tag="VIX62_10665"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY5860738.1"
                     /translation="MRDRQKIRSKMHREDGAAAVEFALIVGLLAMLVFGMMEYGLAFW
                     QLQSLRAATREGARVAAVGGDTSQVTNAVVDASSGALPAGFGGISVSGGGCPEPSAGT
                     PIDQSVTVSINNAALPANIQEILTIDVPLLPSITLDPTIEGTFRCEPV"
BASE COUNT          376 a          882 c          849 g          444 t
ORIGIN      
        1 gaccgggttc tggatgttga gcacccgcgt ggcctgtacg accgtcacgg tgaacttgaa
       61 cgggatcgcc acgagcggac cgttggtgct ggtctccgag agcagcttcg acagctgcgc
      121 cggcgacagg atctgccgca gggccgcctt cgggacgatc tcgccggcct tgaaggaggc
      181 gtacaccgcg acgctgtcgc cgcgctggat cacgccgtag ccggcgggac caccttcgag
      241 caccatcggc agacccacgt tgccgggggt gatgccgagg gggccacccg tcggagcctc
      301 gccgctcgac agccgcgggc tcgcgatctg ctcgttcgcc aggatcggcg ccgtcgtggt
      361 ctgcccacgc agctcgctca tgctcgtgac cgcgccgtcg acgaccgctt cggtcggcac
      421 acggagctcg gtgaacgcgc cctcttccag cagcgggttc aggttcgtgt tggccgcgat
      481 gtcctgcttg gacacgacca cgacggagag cgctccgccg gtcacggcat cgttcttgac
      541 accgttcgtg tagaggatca ccgctgccgc cgccacgatg gcgaggacca cggcgatcgc
      601 gaccaccaga ccgcgtgacc tcatctccct agctcctttc ctgagccctt cagctctctt
      661 cggggcatgt ccccagttcg aggtcgcaga gcgcgaacgc gctcaccccg aagttctgtc
      721 cgctgccgat cgcgcccgac gaggtggtga aaccgcgcca ctccgtcacg atgcagatcg
      781 cgttcggatc gcttcgacgg gtgccacagg caccctccgt cccattcatc gaccagccgt
      841 actcaacctt gaggtccgtc tcgttcggct ggcgccaggt gatctggcga gccaccgggt
      901 cgtaccagta gccacaggtg gcactgatgt cgccgggggt gcactgctgg tactcaggac
      961 ctttcttggg gtagacgtgc acgtcggcgg cgttcaggtt gtcgggcgtg cccgtggggc
     1021 acccgccgcc cgtgccgtag gtgtcggaca gaggcttgac ctggccgttg gtgaggctcg
     1081 ccttcgacag cgtgcaggtg ccgctcgcgc cggtggtgcc gatcgcggcg gggtcatcgc
     1141 cctcgtagac ctcgatcagc ttcagccggg tgaacccgat gatgtcgtac ttgtcgggcg
     1201 tcgaggggca cggcgagacg ctgccgctct tgtcgagctg accctcgcag tcgttcacgg
     1261 ggaacagcac gaccgcgttc gcctccatgc gatccaccaa gttctgccag ttcgaggtgc
     1321 tgtggccggt gtcgttgcag acgtaggtgg gctggctgcc cggtggcgtg ccgttcagca
     1381 cgagcgggtc gccgtagtcg ttcaggatcc agtccgcacg atcgctgctg ccggcactgt
     1441 ggcagttctc gccggcgtcg acgttccaca tgtcgaggtt catgaagccc cagttcgcct
     1501 cgccgaggct cgcgtcgccg ttgttgtagt acatggcaca ggtgtcgccg atcgacacgc
     1561 cgtcggggac cttgcagatg ccctgcaggt agcccgactc gagcacgatc ggcaccgcgt
     1621 acccaccggc ggcggggccc caagcggccg tggccaccgt gctcacgtcg ccggtgctgc
     1681 cgaaccccag cacccccgcg aagaacaggt tctgctgggt gaggtactcg acgctcacgt
     1741 ggccgttccc gtcgtcgcag ccgacgatct cggtgatgtt cgaggtgccg gtgatcacgc
     1801 cgtcgacgtt gtcggccgcg tacgtgtcgg ccaccgactc cgggtcgagc gtgtcctcgg
     1861 cgctcgcaca cgactgcgcg gccgcgagcg cggccgagtc ggaggcgttc accatcgcgc
     1921 gccgggcgag cagcaagccg ccgacgtcca ccgtcaggac gagcatcgcg aagatggcga
     1981 tcaatgagag caccacgatg acgacgaccg cgcccgattc ctcacccgag cgctggcggg
     2041 cgtcgcggat gcgagcgatc agaccggctc gcatcggaag gtcccttcga tcgtcggatc
     2101 gagcgtgatc gacggcagca gggggacgtc gatcgtgagg atctcctgga tgttcgccgg
     2161 caacgcagcg ttgttgatcg acacggtgac cgactggtcg atcggcgtgc cggcgctcgg
     2221 ctcggggcac cctccgccgc tcaccgagat gccgccgaac cctgccggca gggcgccgga
     2281 ggaggcgtcg acgaccgcgt tcgtgacctg cgacgtgtcg ccgcccaccg cggcgacgcg
     2341 agcgccttca cgggtcgccg cccgcagact ctgcagctgc cagaacgcga ggccgtactc
     2401 catcatgccg aacacgagca tcgcgagcag gcccacgatc agggcgaact cgaccgccgc
     2461 cgcaccgtct tcgcggtgca tcttcgatcg gatcttctgt cgatctctca tcggcttggc
     2521 acctcactcg tccgccggtc cctcgctcgt t
//
LOCUS       DATDBZ010000450         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_774759, whole genome shotgun sequence.
ACCESSION   DATDBZ010000450 DATDBZ010000000
VERSION     DATDBZ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774759"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VIX62_10670"
     CDS             complement(<1..788)
                     /locus_tag="VIX62_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY5860739.1"
                     /translation="MSTAAAVVLFIGVLAYAIFGGADFGAGFWDLVAGGTERGGRPRT
                     VIDHSIGPVWEANHVWLIFTFVVLWTCFPEAYASITLTLFVPLTIAALGIVLRGSSFA
                     FRKAVLRTSYRRNLGAIFAISSVLVPYCLGAVVGGIASGRVPAGGVAGDPWNSWVNPT
                     SILGGCLAVVVAAYLASVYLIWDARRLSDDEMVAYFRRRAAAVAVIAGVMSIVGIFVL
                     RADARYLFDGLTSRGLPFVVIAAVAGAGSLWALLRASPTGARILA"
     gene            complement(785..2206)
                     /locus_tag="VIX62_10675"
     CDS             complement(785..2206)
                     /locus_tag="VIX62_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020127436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY5860740.1"
                     /translation="MIDPGFVHGVLAVNPVPWARAQMAFTLACHIILVPLGVSWALMV
                     LIANYRGLKRDDAAALELAQRWSKYMAVTFAVGAVSGTVLTFEFGLLWPRFMGRWGDA
                     FGIPFAFEGVFFFMEAIFVSIYIFGWRRLKPWTHFWTGVPIVVAGFFGAASVVAANAW
                     MNAPSGFTLDKSGTIVEVDAVSVIFNDAMPLMAAHMLVAAYVVGGFLVASVYAFGMLR
                     GRRDRHHRLGFIIAFSVAAVAIPLQLAVGDSLARWVYNNQPVKFAAIELVPTTKSDVP
                     ETLLGHLNEDGTVSGGIPIPGLASWLSDPSTGTKTVVQGLDTVPKDQRPTIREVNTVH
                     LAWDVMVGLGTLLLLLALWYFASWIFRRDMPKSRWFLRIATCAGVLAILAMEAGWVVS
                     EVGRQPWIVYEYMKVEDAATGNTGVWITFVAVVLLYIGVAVTTILVLRGMSRRFREGG
                     APDEDVPYGPRVSSASTTGERDP"
     gene            2375..2857
                     /locus_tag="VIX62_10680"
     CDS             2375..2857
                     /locus_tag="VIX62_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860741.1"
                     /translation="MGLAWPVRSVIAGFAGTAAMALAYRTEHALRPAVHGQLDYDDSL
                     VPGEIVASVLRLSDVTERGDAELGTALRWGYGSAFGLWHGVLRRRLPEPWASLIFGAT
                     LMSATFSLFPLLGRTPPPWRWPRDVLATSLATHAAYVLTVATVDAGFDGSRAEGEPST
                     "
BASE COUNT          556 a         1054 c          949 g          416 t
ORIGIN      
        1 gcaaggatgc gcgcacccgt cggcgatgcc cggagcagtg cccacaacga gcccgcgccg
       61 gcgaccgcgg cgatcacgac gaacggcagc ccgcgtgacg tcaacccgtc gaacaggtat
      121 cgagcatcgg cccgaagcac gaagatgccc acgatcgaca tgacgcccgc gatcaccgcg
      181 acggcggcgg cgcgacgtcg gaagtaggcc accatctcgt cgtcggagag acggcgcgcg
      241 tcccagatga ggtagaccga ggcgagatag gcggccacga ccacggccag gcatcctccg
      301 aggatggatg tcgggttgac ccaactgttc cacgggtcgc ccgccacgcc tcccgcgggc
      361 acgcggccgg aggcgatgcc ccccacgacc gcccccaggc agtagggcac cagcaccgac
      421 gagatcgcga agatggcgcc gaggttccgc cgatagctgg tgcgcagcac cgccttccgg
      481 aaggcgaagc tcgaaccacg gaggacgatg cccagtgcgg cgatcgtcag cggcacgaat
      541 agcgtgagcg tgatcgatgc atacgcctcc gggaagcagg tccacagcac gacgaacgtg
      601 aagatgagcc acacatggtt cgcctcccac acgggcccga tcgaatgatc gatcaccgtt
      661 cgcgggcgac ccccgcgctc ggttcctccg gcgacgaggt cccagaaccc cgccccgaag
      721 tcggcgccgc cgaagatcgc gtacgcgagc acgccgatga acaacacgac cgccgcggcg
      781 gtgctcacgg gtcccgctcc cccgtcgtcg acgccgagct cacgcgcgga ccgtagggaa
      841 catcttcatc gggggcgccg ccctctcgga accggcggct catgccacgg agcacgagga
      901 tcgtggtcac cgccacaccg atgtacagga gcacgaccgc gacgaaggtg atccagacgc
      961 cggtgttccc ggtcgcggcg tcctccacct tcatgtactc gtagacgatc cagggctgcc
     1021 ggcccacctc gctcacgacc caccccgcct ccatcgcgag gatcgccaga acgcccgcac
     1081 aggtggcgat ccgcaggaac catcggctct tcggcatgtc gcgccggaag atccacgatg
     1141 cgaagtacca gagcgcgagg agcaacagga gcgtgcccag ccccaccatg acgtcccagg
     1201 cgaggtggac cgtattgacc tccctgatcg tgggccgctg atccttgggc accgtgtcga
     1261 gcccttgcac aacggtcttc gtgcccgtgc tcggatccga gagccacgag gcgagaccgg
     1321 ggatcgggat gccccccgac accgtgccgt cctcgttgag atggccgagc agggtctccg
     1381 ggacgtcgct cttggtggtc ggaaccagtt cgatcgccgc gaacttcacc ggctggttgt
     1441 tgtacaccca tcgcgcgagc gagtccccga ccgccaactg cagcgggatc gccaccgcgg
     1501 cgacggagaa cgcgatgatg aagcccagcc ggtgatgccg gtctcgtcgt ccgcgcaaca
     1561 tgccgaaggc gtacaccgac gccaccagga atccgccgac cacgtatgcg gccacgagca
     1621 tgtgcgccgc catgagcggc atcgcgtcgt tgaagatcac ggagaccgca tccacctcca
     1681 cgatcgtgcc gctcttgtcg agggtgaacc cggagggcgc gttcatccat gcgttggcgg
     1741 ccacgaccga cgcggcgccg aagaacccgg ccaccacgat cggcaccccg gtccagaagt
     1801 gcgtccaagg cttgagcctg cgccagccga agatgtagat cgagacgaag atcgcctcca
     1861 tgaagaagaa gacgccttcg aacgcgaacg ggataccgaa cgcatccccc caccggccca
     1921 tgaagcgcgg ccagagcagg ccgaactcga acgtcagcac cgtgccgctg acggccccga
     1981 cggcgaacgt cacggccatg tacttggacc agcgctgcgc cagctcgagc gcggccgcat
     2041 catcgcgctt cagtccccgg tagttcgcga tgaggaccat caaggcccac gaaaccccga
     2101 gcggcaccag gatgatgtgg caggcgagcg tgaacgccat ctgcgcgcga gcccacggca
     2161 ccgggttcac cgcaaggacc ccgtgaacga atcccggatc gatcatcgcc actcccgcac
     2221 gtcgatgttc ccctccgtcg agcccgcggc gatgtgacca tacacaggtc tcgatgacgc
     2281 catccgccac cgaacgttca cctgcgctcc gtagggtggt tgaactgcgt gtcgtgtgga
     2341 aggcttgagt tccagcgaac ggaagggcaa cgacatgggt ctggcatggc cggtccgaag
     2401 cgtcatcgcc gggttcgcgg gcacggcggc gatggcactc gcctaccgca ccgaacacgc
     2461 cctgcgcccg gccgtccatg ggcaactcga ctatgacgac agcctggtcc cgggcgagat
     2521 cgtcgcgagc gtcctgcgac tgtccgatgt gactgaacgc ggcgacgccg aactcgggac
     2581 agccctgcgg tggggatacg gatccgcctt cggcctctgg cacggtgtgc tccgacggag
     2641 gttgccggag ccgtgggcca gcctgatctt cggggccacc ctgatgtcgg cgacgttctc
     2701 gttgttcccc ttgctcgggc gaacgcctcc gccgtggcgg tggcctcgcg acgtcctggc
     2761 gaccagcctg gccacccacg ccgcctacgt cctcaccgtc gcgacggtgg acgccgggtt
     2821 tgacggatct cgtgcagagg gggagccttc gacctgaccc gccaagcgtt gccgtgtacc
     2881 cgatctgatc gccaacccgc aggagagatc gatgtcgaac gacacgtcgc accacacctc
     2941 tactgatgcc cgccccgctg atgtcttggt ggtgt
//
LOCUS       DATDBZ010000451         7298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1159291, whole genome shotgun sequence.
ACCESSION   DATDBZ010000451 DATDBZ010000000
VERSION     DATDBZ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159291"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..556)
                     /gene="raiA"
                     /locus_tag="VIX62_10685"
     CDS             complement(182..556)
                     /gene="raiA"
                     /locus_tag="VIX62_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY5860742.1"
                     /translation="MDLTFTGRDLQVTDEIREVAGHKLSRLERMEPRAVRIDLEFIAE
                     HYPRLDGLKRVEAALFIPRKTFRAHAEADDVPTALDRVADKLERQLRDHHGKRRASVH
                     KAGLESAHLNPEPADNDESDGD"
     gene            complement(869..1537)
                     /locus_tag="VIX62_10690"
     CDS             complement(869..1537)
                     /locus_tag="VIX62_10690"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HEY5860743.1"
                     /translation="MLARALDVIFPQRCAGCGAGPWPFCDPCRASLKVLRPPWCRRCG
                     RPKAMSVPRCRDCPPPEIDTARAPFRFEGPARQAIHRLKFSGWRGVGSALSLAMTEVE
                     GLPGVEVVTWVPLARRRLAERGFDQARVLATGVARRTETPCVGLLTRARSTGPQAKRD
                     AVARRMAMRGAFAVADRVEVPSSVLVVDDVLTTGATAAAAAQALRQAGATSVHLVVAA
                     RAYS"
     gene            complement(1610..2236)
                     /locus_tag="VIX62_10695"
     CDS             complement(1610..2236)
                     /locus_tag="VIX62_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860744.1"
                     /translation="MDGEISIRAHYERFPATIKGAFVLRGEGRDPRQVRIEDARVIEV
                     SGGGSQSIAMEPVTLEVAPHLDLFVPFEVPLTELGAGWYSLECDVVIDGVPDVVHPGD
                     RFPVAWPRATVRRGPIPVGKRLDVSGDTVAIDQIDCAGDSIKIGFTATKAPTLKLTAD
                     GATVPVLDTQFDDATGRGKIVGYPLMRAQRALTIDASGAKRPLEVTLP"
     gene            complement(2247..3503)
                     /gene="ahcY"
                     /locus_tag="VIX62_10700"
     CDS             complement(2247..3503)
                     /gene="ahcY"
                     /locus_tag="VIX62_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY5860745.1"
                     /translation="MTNDVRDMGLAQAGRERIEWAGAEMPVLALIQERFEKEQPLAGI
                     RIGACLHVTTETANLMIALKAGGAEVALCASNPLSTQDDVAAALVEAGIDTYAIKGED
                     TDTFFKHINAVLDIRPNITMDDGADMVSVLHKERVDQIAEIIGGTEETTTGVIRLRAM
                     AKDGALKYPIVSVNDADTKHLFDNRFGTGQSTIDAIMRATNRLLAGRTIVVCGYGMCG
                     RGVASRARGMGAHVIVTEVDPTAAIEAVMEGYRVMPIRDAARVGDIFITLTGDTSVVR
                     KEHMELMKDGAMLANSGHFDVEIDKPGLAELGVGGVRRIRDFVDEYTLADGRKLYLLG
                     EGRLVNLSAAEGHPAAVMDMSFANQALSAEWVVANKDSLEPRVYPVPPENDKEVARLK
                     LHAMGVEIDELTPEQDHYLHSWEQGT"
     gene            complement(3532..4353)
                     /locus_tag="VIX62_10705"
     CDS             complement(3532..4353)
                     /locus_tag="VIX62_10705"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY5860746.1"
                     /translation="MTVFAPADDLAAQGAGVVRSVTDLVPGAGIVLGSGLGPALGEDL
                     EEIASFAYTDLPGFPPPGVPGHEGKLVLGRIAGVPVAGFFGRVHFYEGHGMDASALLP
                     RLAKELGADTMVVTAAVGGVVPGLAAGTVVVLRDHLNLMGEAPLRGWRAPDGMPAFIS
                     TTNVYDAGLRTLALERAAALGIPAAEGVYAAMSGPAYETPTEVGFLQQAGATVVGMSM
                     VPESLPSRALDMRVLGLCSLTNAFGEHVEHDEVVRVSNETAVAVGRLLVDLLPQM"
     gene            complement(4350..5420)
                     /locus_tag="VIX62_10710"
     CDS             complement(4350..5420)
                     /locus_tag="VIX62_10710"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEY5860747.1"
                     /translation="MTIDLDDVEAITPIDPGDMLGAITAMPQHVRTGFAAGNEAPGMP
                     QADGVTSIVFAGMGGSAIAGDVLRTLARDRLTVPVDVNRGPVLPVFCGPHTLVICSSY
                     SGNTAETLAAYADARERGCRIVAITSGGELATRASADGQGIVPVPGGYVPRAAFGLIV
                     FATLGAVGAVGLLPPAEPEVAQAADELERLMIALAPGVPRADNPAKELAWNLGDRQPV
                     IWGAEGWAATAAARWKGQFNENAKILAWSSAMPELDHNEVEGWAPRQGEGSFVIALRH
                     EGEHPEIAMRFEPSLAIARDAGAVTDEVWAAGSSELSRFLSLVAMGDLASTYHAIGRG
                     VDPTPMDAIMRLKAYLAGRVGS"
     gene            complement(5417..5656)
                     /locus_tag="VIX62_10715"
     CDS             complement(5417..5656)
                     /locus_tag="VIX62_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY5860748.1"
                     /translation="MSLDPQLLEILVCPNDRGDLDYREDESVLVCTTCGYRYPIRDDI
                     PVMLIDEAEKPDKPSKKPAKKPAQKPAQKPDGGGR"
     gene            complement(5661..7007)
                     /gene="manB"
                     /locus_tag="VIX62_10720"
     CDS             complement(5661..7007)
                     /gene="manB"
                     /locus_tag="VIX62_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846775.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY5860749.1"
                     /translation="MPSALDRIFKAYDVRGVVPDDLDAALVRRIGAAFADWSRAPAIA
                     LGRDCRLSSPELAAALADGITARGADVIDLGLASTDLLYFASGSLDVPAIMLTASHNP
                     KQYNGLKFCLAGAKPVGEDTGLREIRSLTEGPDPGPVASPGTVQERDLLAAYVDHVLS
                     FVDVEAMRPMTVVADTANGMGGLVVPAVMARLPITLHHLYAELDGTFPNHPADPIDPE
                     NQKDLKAAVLEHGADVGLAFDGDADRVFLIDEQAQDVSGSLLTALVAIAMLDQEPGGK
                     VVHNLICSWTVPEVVREHGGEPIRTRVGHSFIKQVMADTGAIFGGEHSGHYYFRDNYR
                     ADSGLIAAVVAMGELSKADAPLSSLLAPLRRYADSGEINSKVDDPTERIEAIAAAFDA
                     GRQDRLDGLTVEFEDWWCNVRASNTEPLLRLNVEARTPELLEAKTAEVLALIRGER"
     gene            complement(7064..7240)
                     /locus_tag="VIX62_10725"
     CDS             complement(7064..7240)
                     /locus_tag="VIX62_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860750.1"
                     /translation="MSNGTCPSCHAGDLIKISMTVGGRDLAFSTCHHCEAKWWYREGE
                     EVPLASVIGLVVEK"
BASE COUNT         1140 a         2645 c         2341 g         1172 t
ORIGIN      
        1 cttgcgccac atcgacggct tctgcaccgt cggaggcctc gcccacgagc tcgatgtcgt
       61 cctcctggga gagcaccatc tcgagcccac gcctgaacaa cgcgtggtcg tccaccacca
      121 ccacacgcag gcgctcggac ccctcgcctc cgctcgtttc gttcaccgtc acgattcccc
      181 ctcagtcccc gtcgctctcg tcgttatcgg caggctccgg gttcaagtga gcggattcta
      241 gccctgcctt gtgcacggat gcccgtcgtt tgccgtgatg atcgcgaagt tgccgctcca
      301 gcttgtccgc gacacggtcc agggcggtgg gcacatcgtc ggcttcggcg tgggcgcgga
      361 aggtcttgcg agggatgaac aaggccgcct ccacccgctt gaggccgtcg agccgggggt
      421 agtgctcggc gatgaattcg agatcgatgc ggacggcgcg aggctccatg cgctccagcc
      481 gggagagctt gtggccggcg acctcacgga tctcgtccgt gacctgcagg tcgcgacccg
      541 tgaaggtcaa gtccatgtcc ggcgatcgcc tcctcgcgtt cacgtgacgg gcgggccctc
      601 gcccggctga cctggtcttt gcccccacac ccgcctgctg gaacgttcgc ctcccggctc
      661 cggagaaccg aacgactccc gccctctctc ggcgaaccga ggcagggctt acatctaaac
      721 cgcgccatgc tcagcgacca agggtcgcct tacgtgggtc gtttcgcccg cggctggcgt
      781 cgactaccgg agatgatctc cgggcaacca cagacccggg cgagggcccg gcccgagtat
      841 aggcatcagg cctcacccct gagaacgctc aggaatatgc tcgagcggcc accaccaggt
      901 gcaccgaggt cgctcccgcc tgtcgcaacg cctgcgccgc agcggctgcc gtggcgcccg
      961 tcgtcaagac gtcgtcgacc accagcaccg atgacggcac ctcgacgcga tcggcgaccg
     1021 cgaacgctcc gcgcatcgcc atcctgcgag cgaccgcatc gcgtttggcc tgcggcccgg
     1081 tggaccgagc cctggtgagc aggcccacgc agggcgtctc cgttcggcgt gccacgcccg
     1141 tggccaggac gcgcgcctgg tcgaacccgc gctcggcgag tcgccgccgt gcgagcggca
     1201 cccacgtcac cacctcgacg ccgggcaaac cttcgacctc ggtcatcgcc aacgacaacg
     1261 ccgagcccac gccgcgccac cccgagaact tcaaccggtg gatcgcctgg cgggccggcc
     1321 cttcgaaccg gaacggtgcg cgagccgtgt cgatctcggg aggcgggcaa tcgcggcatc
     1381 gagggaccga catcgccttc ggtcgtccgc atcggcggca ccatggcgga cggagcacct
     1441 tcagggaggc acggcatgga tcgcagaacg gccacggccc ggcgccgcat cccgcgcaac
     1501 gctgcgggaa gatgacgtcc aacgcccgcg ccagcatcgg tggaacctac cgacgcgcac
     1561 cgacagcggc gccgctcgac gatctacgcc aacaacctag accaacgatc tagggaagcg
     1621 tgacctccag cggacgcttc gcgccactgg catcgatcgt caacgcgcgc tgggctctca
     1681 tcaaggggta gcccacgatc ttgccgcgcc ccgtcgcatc gtcgaactgc gtgtcgagca
     1741 ccggcaccgt cgctccatcg gccgtcagct tcaacgtggg tgccttcgtc gccgtgaacc
     1801 cgatcttgat cgagtctccg gcacagtcga tctgatcgat cgccaccgtg tcacccgaca
     1861 cgtccaggcg cttgccgacc ggtatcggac cacggcggac cgtcgcgcgc ggccaggcaa
     1921 ccgggaaccg gtcgcccgga tgcaccacgt ccggtacgcc gtcgatcacc acatcgcact
     1981 ccagcgaata ccagcccgcc ccgagctcgg tgagcggcac ctcgaacggc acgaacaggt
     2041 ccaggtgcgg cgccacctcc agcgtgaccg gctccatggc gatcgactgc gatcccccgc
     2101 ccgacacctc gatcacccgc gcatcctcga tccggacctg ccgcgggtcg cggccctcgc
     2161 cacgcagcac gaacgccccc ttgatcgtgg cggggaaccg ctcgtagtgc gccctgatgg
     2221 agatctcgcc gtccatttct cgacccctag gtcccctgct cccagctgtg caggtagtga
     2281 tcctgctccg gcgtgagctc gtcgatctcc acacccatcg catggagctt cagcctggcg
     2341 acttccttgt cgttctccgg cggcaccggg tagacgcgcg gctcgagcga gtccttgttc
     2401 gccacgaccc actccgccga gagcgcctgg ttcgcgaacg acatgtccat gaccgccgca
     2461 gggtggcctt cggcggccga caggttgacg agccggccct cgcccagcag gtagagcttc
     2521 ctgccgtcgg ccagcgtgta ctcgtcgacg aagtcgcgga tccggcgcac gccacccacg
     2581 ccgagttcgg cgaggccggg tttgtcgatc tcgacgtcga agtgccccga gttcgcgagc
     2641 atcgcgccgt ccttcatcag ctccatgtgt tccttgcgga ccacgctggt gtcgcccgtc
     2701 agcgtgatga agatgtcgcc cacgcgcgcg gcgtcgcgga tcggcatcac ccggtagccc
     2761 tccatcacgg cctcgatcgc cgcggtgggg tcgacctcgg tgacgatcac gtgcgcgccc
     2821 atgccacgag cccgcgaggc gacaccgcga ccgcacatgc cgtagccgca caccacgatc
     2881 gtgcgtccgg ccagcagccg gttcgtggcg cgcatgatcg cgtcgatcgt cgactgtccc
     2941 gtgccgaacc ggttgtcgaa caggtgcttg gtgtcggcgt cgttcacgct cacgatcggg
     3001 tacttcagcg cgccgtcctt cgccatcgcc cggagacgga tcaccccggt ggtggtctcc
     3061 tcggtacccc cgatgatctc ggcgatctgg tcgacccgct ccttgtgcaa cacgctcacc
     3121 atgtcggcgc cgtcgtccat cgtgatgttc ggccggatgt cgagcacggc gttgatgtgc
     3181 ttgaagaacg tgtccgtgtc ctcacccttg atcgcgtagg tgtcgatccc ggcctcgacc
     3241 agcgcggccg ccacgtcgtc ctgtgtggac agcgggttcg aggcgcacag ggccacctcg
     3301 gcacccccgg ccttcagcgc gatcatcagg ttggccgtct cggtggtgac gtgcagacac
     3361 gccccgatac ggatccctgc gagcggctgc tccttctcga agcgctcctg gatcagcgcc
     3421 aggacgggca tctcggcccc ggcccactcg atgcgttccc ggccggcctg cgccagcccc
     3481 atgtcccgta catcgttcgt catcgtccga tccttcccct cacccacatc gtcacatctg
     3541 tggcagcagg tcgaccagca gccgccccac cgccaccgcg gtctcgttcg agacccgcac
     3601 cacctcgtcg tgctcgacat gttcgccgaa cgcgttcgtc agcgagcaca gacccagcac
     3661 ccgcatatcc agggcacgcg acggcagcga ctccggcacc atcgacatcc ccaccaccgt
     3721 ggcgcccgcc tgctgcaaga accccacctc ggtgggcgtt tcgtaggcgg gtccgctcat
     3781 cgccgcgtag acaccttcgg ccgcggggat cccgagcgcg gcggcgcgtt cgagcgccag
     3841 ggtgcgcaaa ccggcgtcgt acacgttcgt cgtactgatg aacgcgggca tcccgtcggg
     3901 tgctcgccac ccgcgcagcg gagcctcccc catcaggttg agatggtcgc gcagcaccac
     3961 caccgtgcct gccgcgagac cgggaacgac gccacccacc gccgccgtca ccaccatcgt
     4021 gtcggccccg agttccttgg ccaatcgagg cagcaaggcg gaggcgtcca tcccgtgtcc
     4081 ttcgtagaag tgcacccgtc cgaagaagcc ggcgaccggc accccggcga tccggcccag
     4141 cacgagtttg ccctcgtggc ccgggacccc gggcggtggg aacccgggca ggtcggtgta
     4201 ggcgaagctc gcgatctcct cgaggtcctc gccgagggcg gggcccagcc ccgaacccag
     4261 aacgatgccg gcccccggca ccaggtcggt gaccgaacgc accacgcccg caccctgagc
     4321 cgcgagatcg tcggccggcg cgaagacggt cacgaaccca ccctgccggc caggtaggcc
     4381 ttcaggcgca tgatcgcgtc catcggcgtg gggtccacac cgcggccgat cgcgtggtag
     4441 gtactcgcga ggtcgcccat ggccaccaac gacaggaacc gcgagagctc actcgatccc
     4501 gccgcccaca cctcgtcggt gaccgcaccg gcatcgcgcg cgatcgccag tgagggctcg
     4561 aaccgcatcg cgatctcggg atgctcgcct tcgtgccgaa gcgcgatcac gaacgagccc
     4621 tccccttgcc gaggggccca cccttcgacc tcgttgtgat cgagttcggg catcgccgat
     4681 gaccacgcca ggatcttcgc gttctcgttg aactggccct tccacctcgc cgcggccgtg
     4741 gctgcccacc cctcggcgcc ccagatgacc ggctggcggt cgccgaggtt ccaggccagt
     4801 tctttcgcgg ggttgtcggc gcgcggcact cccggagcga gcgcgatcat caagcgctcg
     4861 agttcgtcgg ccgcttgcgc cacttccggc tcggccggtg gcagcaatcc caccgcgccg
     4921 accgctccca gcgtggcgaa cacgatcagg ccgaaggccg cccggggcac atagccccca
     4981 ggcacgggga cgatgccctg cccatcggcg gaggcccggg tcgcgagttc gccgccggag
     5041 gtgatcgcca cgatccggca tccgcgctct cgcgcatcgg cgtaggcggc gagcgtctcg
     5101 gccgtgttcc cggagtacga cgaacagatc acgagcgtgt ggggaccgca gaagaccggc
     5161 agcaccggcc cccggttgac gtcgaccgga acggtgagac ggtcgcgagc cagggtgcgc
     5221 aagacgtcgc ccgcgatcgc cgatcctccc atcccggcga acacgatgga ggtgacgccg
     5281 tcggcctgcg gcatccccgg cgcttcgttc ccggccgcga atccggtccg gacgtgctgg
     5341 ggcatcgcgg tgatggcccc cagcatgtcg ccggggtcga tcggagtgat ggcctcgacg
     5401 tcgtcgaggt cgatcgtcac cggccgccgc cgtcaggttt ctgggcaggt ttctgggcag
     5461 gtttcttggc aggtttcttg gagggcttgt ccggcttctc agcctcgtcg atcagcatca
     5521 cggggatgtc gtcgcggatc ggatagcggt agccgcaggt ggtgcagacc aacacggatt
     5581 cgtcttcccg gtagtcgagg tcgcctcgat cgttcgggca gaccaggatc tccaacagct
     5641 gagggtcgag gctcatggcg tcagcgttct cctctgatca gcgcgagcac ctcggcggtc
     5701 ttggcctcca ggagctcggg cgtccgggct tccacgttca ggcgcaggag cggctccgtg
     5761 ttcgaggcgc gcacgttgca ccaccagtcc tcgaactcta ccgtcagacc gtccaggcgg
     5821 tcctgtcgcc cggcatcgaa cgccgccgcg atggcctcga tccgttcggt cgggtcgtca
     5881 accttcgagt tgatctcacc ggagtcggcg taccgtcgca agggcgccag cagcgacgag
     5941 agcggcgcgt cggccttcga cagctcgccc atggcaacca cggcggcgat cagacccgaa
     6001 tcggcccggt agttgtcccg gaagtagtag tgacccgagt gttctccgcc gaatatcgcg
     6061 ccggtgtcgg ccatcacctg cttgatgaag gagtgcccca cccgggtgcg gatgggctcg
     6121 ccgccgtgct cgcgaacgac ctcgggcacg gtccatgagc agatcaggtt gtgcacgacc
     6181 ttccctccag gttcctgatc cagcatggcg atcgccacca gggcggtcag gagcgaaccg
     6241 ctcacgtctt gggcctgctc gtcgatcagg aacacccggt cggcgtcacc gtcgaaggcc
     6301 aggcccacgt cggcgccgtg ctccagcacg gcagccttca ggtccttctg gttctcgggg
     6361 tcgatggggt cggcgggatg gttcgggaac gtcccgtcga gctcggcgta gaggtggtgc
     6421 agggtgatgg gcaaccgcgc catcacggcg ggcaccacca gaccacccat gccgttggcc
     6481 gtatcggcga ccaccgtcat gggacgcatg gcctcgacgt ccacgaacga caagacatgg
     6541 tcgacgtagg cggcgagcag atcgcgttcc tgcacggtgc ccggcgacgc gaccggacct
     6601 ggatcggggc cctcggtcaa cgagcggatc tcacgcaggc ccgtgtcctc tcccaccggc
     6661 ttggcgccgg ccagacagaa cttcaggccg ttgtactgct tgggattgtg gctcgccgtg
     6721 agcatgatgg cgggaacgtc gagcgaaccg gacgcgaagt acagcaggtc ggtggaggcg
     6781 agtccgaggt cgatcacgtc ggcgccccgg gcggtgatgc cgtcggcgag cgctgccgcc
     6841 agttcgggcg aacttaggcg gcagtctcgt cccaacgcga tggcgggagc gcgcgaccag
     6901 tcggcgaagg cggctccgat cctgcggacg agcgcggcgt cgagatcgtc aggcaccacg
     6961 ccgcggacgt cgtaggcctt gaagatgcga tcgagagcgc tgggcacgca cgcatcctac
     7021 gagaggatga cgccggcacg ctcccccgag cggaggcggt gggctacttc tcgacgacga
     7081 ggccgatcac ggatgccaga gggacctctt cgccttcgcg gtaccaccac ttggcttcgc
     7141 aatggtgaca cgtgctgaag gcgaggtccc gcccgccgac cgtcatcgag atcttgatga
     7201 ggtcgcccgc gtggcagctg ggacaggttc cgttactcat gctttcccat cggttcgggg
     7261 aggggttcgc gaacatagca cggcccgccc atttcctt
//
LOCUS       DATDBZ010000452         3272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_555559, whole genome shotgun sequence.
ACCESSION   DATDBZ010000452 DATDBZ010000000
VERSION     DATDBZ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555559"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VIX62_10730"
     CDS             complement(<1..748)
                     /locus_tag="VIX62_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(500..502),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5860751.1"
                     /translation="MKNGLTRLTEPMVRDNGELRVATWDEALDRAAQGLRSATERNGG
                     NGVGLFSCSKATNEMNFVAQKFARQVLLSNNIDSCNRTUHAPSVVGLATVFGAGGGTS
                     SYEEIEHTDLILLWGSNARAAHPIFFHHALKGIHNGAQMVVVDPRRTESAEWGDLWLG
                     LDVGSDIALSNSIAREIIVNDLQHHTFIEHATVGFDEYRDSVMDWTLERGEEVTGVPA
                     EIIGEVAHAYAKADRAMICWTLGITEHHNAV"
     gene            879..1973
                     /locus_tag="VIX62_10735"
     CDS             879..1973
                     /locus_tag="VIX62_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860752.1"
                     /translation="MDRIPIGGTPPDPDDGEHDNVVRLPSVQASDEVVGIPVESHQHD
                     DAAVDDEPEMSWEEAVAADAEADVPEDPPPVVPGAGMATLLEQGMRVGAGMVSAGASA
                     VADALRSTMPESADEEGEDPAATMAGAGLGAAITAAEAAASAAKSAAEAVGPIMSWVT
                     NPAALQGASETAAGAARLLDGQWKATQAETAQAATSFLTVLIPEITKAILDQVDLTQI
                     VKERVDINAIVEDVDMDRIVNRLDIAAIVDRVDVNAVVQRVDMEQIVEDLPIEKVVDR
                     VDVNEIVERVDIRGVIDRLDLAEITQDVMDDIDLPSIIRESSGAMASETVQTVRVRGM
                     DADGLVSKIVDRMLRREQRQLEAPGEPKDD"
     gene            1975..2481
                     /locus_tag="VIX62_10740"
     CDS             1975..2481
                     /locus_tag="VIX62_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY5860753.1"
                     /translation="MADTATHPPTHAYPAGARERQGLRAGVVSRTTAMVIDIAYVAVL
                     IGAAYLGYCAFRFLRDPRAFTWPVISSTQFITAALIVAAIALAIGWAGIGRTAGMRIM
                     GLRIVGRSGTTLGFTIALLRAITCVVFPIGLFWSAVSKRNASVHDLVFRTSVIYDWQA
                     RVPRTKRS"
     gene            complement(2478..3248)
                     /gene="fdhD"
                     /locus_tag="VIX62_10745"
     CDS             complement(2478..3248)
                     /gene="fdhD"
                     /locus_tag="VIX62_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY5860754.1"
                     /translation="MRSERPDQLATEEPLEIRVGGPGQEASQVAVTMRTPGGDFELAV
                     GFLFTEGLIGPADVQRVAYCDDVDDETQRYNVVTVSLQRPFDAESVRRNFYATSSCGV
                     CGKAALDDIEVRCAPVATGTRVDAEVLVSLPDRLREKQRVFDRTGGLHAAGLFDVEGR
                     LVSLREDVGRHNAVDKVVGEQLLAGAIPLGDHVLQVSGRASFEIVQKAAVAGIPVVSA
                     VSAPSSLSVEAAERFGMTLVGFVRDGGCNVYSHPERVV"
BASE COUNT          516 a         1137 c         1099 g          520 t
ORIGIN      
        1 cgaccgcgtt gtggtgctcg gtgatgccga gcgtccagca gatcatcgcc cggtcggcct
       61 tcgcgtaggc gtgggccacc tcgccgatga tctcggcggg cacgccggtg acctcctccc
      121 cgcgctccag cgtccaatcc atgaccgagt cccggtactc gtcgaagccc acggtcgcgt
      181 gctcgatgaa ggtgtggtgc tgcaggtcgt tgacgatgat ctcgcgtgcg atcgagttcg
      241 agagcgcgat gtcgcttccc acgtcgaggc cgagccacag gtcgccccac tccgccgact
      301 cagtgcggcg cgggtcgacc acgaccatct gggcgccgtt gtggatcccc ttgagggcgt
      361 ggtggaagaa gatcggatgc gctgctcgcg cgttcgatcc ccacaggagg atgagatcgg
      421 tgtgctcgat ctcctcgtat gaactcgtac cgccacctgc tccgaacacc gtggccagac
      481 cgaccacaga aggagcgtgt caggtgcggt tacatgagtc gatgttgttg ctcagcagca
      541 cctgacgggc gaacttctgt gcgacgaagt tcatctcgtt cgtggccttg ctgcagctga
      601 acaggccgac cccgttgccg ccgttccgct ccgtggcgct gcgcagtccc tgagcggccc
      661 gatcgagggc ttcatcccag gtggcgacgc gcagttcgcc gttgtcgcgg accatcggct
      721 cggtgagtcg cgtgagcccg ttcttcatcg agcggctcct ttcaccggtt gtgcgttcct
      781 atcgtacgtc gggcccggag tggtgggtga acccggactc cagcatcctg accggaggtc
      841 ggtgggcacg gatccccccg cgggttcatg atgggggcat ggatcgcatc ccgatcggcg
      901 gcacgccgcc agaccccgac gacggcgaac acgacaacgt ggtgcgcctg ccctccgtcc
      961 aggcatccga cgaggtggtc ggcatcccgg tggagtcgca ccagcacgac gatgccgccg
     1021 tggacgacga accggagatg agctgggaag aggccgtggc ggctgacgcc gaggccgacg
     1081 tacccgagga cccgccgccc gtggtccccg gcgccggtat ggcgacgttg ctggaacagg
     1141 gcatgcgggt tggcgccggc atggtctctg ccggagcctc cgcggtggcc gacgccctgc
     1201 ggagcacgat gcccgaatct gccgacgaag agggtgaaga ccccgcggcg accatggcgg
     1261 gcgcaggtct gggcgccgcc atcaccgcgg ccgaagctgc agcctccgcg gcgaagtcgg
     1321 ccgccgaggc cgtgggaccg atcatgtcct gggtcacgaa cccggccgcg ctgcaagggg
     1381 cgtccgaaac cgccgccgga gccgctcggc tcctcgacgg acagtggaag gccacgcagg
     1441 ccgagaccgc ccaggcggcc acctcgttcc tcacggtgct gatccccgag atcaccaagg
     1501 cgatcctgga tcaggtcgac ctcacacaga tcgtgaagga acgggtcgac atcaacgcga
     1561 tcgtcgaaga cgtcgatatg gaccgcatcg tcaaccggct cgacatcgcc gccatcgtcg
     1621 atcgcgtcga tgtgaacgcc gtggtacagc gcgtcgacat ggagcagatc gtcgaggacc
     1681 tgccgatcga gaaggtcgtg gaccgcgtcg acgtcaacga gatcgtggaa cgggtcgaca
     1741 tccgcggggt catcgatcgc ctcgacctgg ccgagatcac gcaggacgtg atggacgata
     1801 tcgacctgcc gtcgatcatc cgcgagtctt cgggcgcgat ggccagcgag acggtacaga
     1861 ccgttcgggt ccgcggcatg gatgccgacg gcctggtgtc caagatcgtc gaccggatgc
     1921 tgcggcgcga gcaacgccag ctcgaagctc ccggcgagcc caaggacgac tgacgtggcg
     1981 gacacggcga cccatccacc gacgcacgcc tacccggcgg gcgcccgcga gcggcagggg
     2041 ttgcgcgccg gcgtcgtgtc caggaccacc gcgatggtga tcgatatcgc gtatgtggcc
     2101 gtgctgatcg gcgcggccta cctgggctat tgcgcgttcc gcttcctgcg cgacccgagg
     2161 gcgttcacgt ggccggtcat ctcctcgacg cagttcatca ccgcagcgct catcgtggcc
     2221 gcgatcgccc tcgcgatcgg ctgggcaggg atcggacgca ccgccgggat gcggatcatg
     2281 ggcttgcgca tcgtgggccg ctcgggcacc accctgggat tcacgatcgc gctcctgcgt
     2341 gcgatcacct gcgtggtgtt cccgatcggc ttgttctggt cggccgtcag caaacgcaac
     2401 gcctcggtgc acgacctggt cttccgaacc tcggtgatct acgactggca ggcgcgcgtg
     2461 ccgcggacca agcggtccta gacgacgcgc tcggggtggc tgtacacgtt gcagcccccg
     2521 tcacggacga accccaccag cgtcatcccg aagcgttccg cggcctcgac cgacagcgac
     2581 gacggcgcgc tgacggcgct caccacgggg atcccggcca ccgcggcctt ctgcacgatc
     2641 tcgaagctgg cgcgcccact cacctgcaac acgtggtcgc cgagcgggat cgcacccgcc
     2701 aggagctgct cccccaccac cttgtcgacc gcgttgtgcc gcccgacgtc ttcccggagg
     2761 gagacgagcc tgccctcgac gtcgaacagc cccgcggcgt ggagtcctcc ggtacggtcg
     2821 aacacgcgct gcttctcgcg caggcggtcg ggcagcgaca ccagcacctc ggcgtccacc
     2881 cgcgtcccgg tggcgacggg cgcgcaccgc acctcgatgt cgtccagggc cgccttcccg
     2941 cagaccccgc acgaggatgt cgcgtagaag ttccggcgca ccgactcagc gtcgaacggg
     3001 cgctgcaggc tgaccgtcac cacgttgtag cgctgggtct cgtcgtcgac gtcgtcgcag
     3061 taggccaccc gttgcacatc ggccggaccg atcagcccct cggtgaacag gaacccgacc
     3121 gccagctcga agtctccacc gggggtccgc atcgtgacgg ccacctgcga ggcctcctgc
     3181 ccgggtccgc cgacgcggat ctcgagcggt tcctcggtgg cgagctggtc cggtcgttcc
     3241 gatcgcaccc cgttacggag cgcggtgaga cg
//
LOCUS       DATDBZ010000453         4302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1792992, whole genome shotgun sequence.
ACCESSION   DATDBZ010000453 DATDBZ010000000
VERSION     DATDBZ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792992"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VIX62_10750"
     CDS             <1..270
                     /locus_tag="VIX62_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEY5860755.1"
                     /translation="VVVGFYSLGLRLLVRAGRAPVVSPAEFTDAITVITVKDAQRAAK
                     AAAKASRKSPLTEGQKQIAAVGAYLSFGVCALAVLGGLLLILFNH"
     gene            313..1224
                     /locus_tag="VIX62_10755"
     CDS             313..1224
                     /locus_tag="VIX62_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEY5860756.1"
                     /translation="MKEASVQFGQHPPARRTILHLSDTHLLAGNVPLGGRYDTAANLR
                     RTLDAVEGLGIRPDAIVFTGDLTDLGEPEAYRALRDAIEPVAERLAAPVIWVAGNHDE
                     RPALRRDLLRLEPSEEPVTAVHDLGGLRLIVLDTTVPGWHHGDLDDAQLEWLQEKLAT
                     AAPLGTILAMHHPPLPSHIPLFDILELRDQSRLARVVTGTDVRAILAGHLHYSTSGTF
                     AGIPVSVSAATCYTMDLARPAAEVNGMDAGQSFHLVHVYDDTITHAVVPVVDALTGEY
                     FSAEWVERMAALTPAERLEAFSRKPGR"
     gene            1274..1657
                     /locus_tag="VIX62_10760"
     CDS             1274..1657
                     /locus_tag="VIX62_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5860757.1"
                     /translation="MTLIHDGVAISEAASATGLSRHTLRYYERAGLMLEPVDRASSTH
                     RRYSEADIGWVQFLTRLRSTGMPIATIREYTDLVRRGDVTIDARRELLVRHRIAVLHQ
                     LGVIQASLAAIDIKIAIYEEKVAHA"
     gene            1654..2631
                     /locus_tag="VIX62_10765"
     CDS             1654..2631
                     /locus_tag="VIX62_10765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020098273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5860758.1"
                     /translation="MKKITLGTDGPVIGRIGLGLMGMSAFYTGAAKDDAGAIRTIQRA
                     LEVGVTFLDTAEIYGPYVNEELLGRALGGRRDEVVIATKFGTIRHSIDDKGGLDGSPA
                     NVRLSVEGSLRRLGTDHIDLYYQHRMDPGTPIEETVGALAELIAEGKIRHYGLSEAAP
                     ATIRRAHAVHPVTALQTEYSLWSRDPEAEILPTVRELGIGFVPYSPLGRGFLTGAIRS
                     LDQLDEGDFRKSNPRFADGNLDANLVIVEKVESVAAEIGATPAQVALAWLLAQGDDIA
                     PIPGTRRIAALEENVGADAVVLSPEHLAALASLDAAAGDRYADMSPLTR"
     gene            2771..4237
                     /locus_tag="VIX62_10770"
     CDS             2771..4237
                     /locus_tag="VIX62_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEY5860759.1"
                     /translation="MALDAMTRTRIETDSLGSMEIPADAYWGIHTARALENFPVSKRP
                     ISVYKDLVRALAMVKQASARANRDIGALDAYKADLIDRASQLVIDGEYHDQFVVGVIQ
                     GGAGTSTNMNANEVITNIALELAGREKGDYDFLSPIDDTNRSQSTNDVYPTAVKVGLG
                     LDLRTLLDELDLLRRSFLAKAVEFHDILKVGRTQLQDAVPMTLGQEFNGFATTLGEDH
                     SRLKENAYLLCEINMGATAIGTGITTHPEYGKSVLRHLREISGLDLDTATDLVESTSD
                     TGAFMSFSSTLKRNAIKLSKICNDLRLLSSGPQAGFGEINLPARQAGSSIMPGKVNPV
                     IPEVVNQVAFAVVGADMTVTMAVEAGQLQLNAFEPVIAHSLFQSITWMRRGMHTLRVN
                     CVDGITANTERLGAMVGASVGVITALTPFIGYAAAAALAKTALLTGRNVADLVVEAGL
                     MSREECMKQLSPARLSGLETVTAAIPVVYADDSGETDE"
BASE COUNT          728 a         1547 c         1378 g          649 t
ORIGIN      
        1 gtcgtggtcg gcttctactc cctcggtctg cgactcctcg tgcgggcggg ccgggcacca
       61 gtggtgagcc ccgccgagtt caccgacgcc atcaccgtca tcaccgtcaa ggacgcccag
      121 cgagcggcca aggcggccgc gaaggcatcc cggaagagcc cgttgaccga gggccagaag
      181 cagatcgccg ccgtcggcgc gtacctatcg ttcggagtgt gtgcgctcgc ggtgctcggc
      241 gggctcctgc tgatcctctt caaccactga ccttcgcggt aaccgcgtcg ggagcgcgca
      301 taggctgagg tcatgaagga ggcatccgtt cagttcggtc agcacccgcc cgcgcggcgc
      361 accatcctgc acttgagcga cacgcacctg ctcgcgggaa acgtcccgct cggcgggcgc
      421 tacgacacgg cggccaacct gcggcgcacg ctcgacgcgg tcgagggact cggcatccga
      481 cccgatgcga tcgtgttcac cggcgacctg accgacctcg gcgaacccga ggcgtaccgt
      541 gcgctgcgcg atgccatcga gcccgtggcc gagcgcctcg ccgcacccgt gatctgggtc
      601 gccggcaacc atgacgaacg ccctgctctg cgccgcgacc tgctgcgcct cgagccgagc
      661 gaagagccgg tgaccgccgt gcacgacctc ggcggactgc gcctcatcgt gctggatacc
      721 accgtgcccg gctggcacca cggtgacctg gatgacgcgc agctggagtg gctgcaggag
      781 aagctggcca cggccgcgcc gctcgggacg atcctcgcga tgcaccaccc tccgctgccc
      841 tcccacatcc ccctgttcga cattctcgag ctgcgcgacc agagtcgcct cgctcgcgtc
      901 gtcaccggaa ccgatgtgcg cgcgatcctc gccggccacc tgcactactc gacgagcggc
      961 accttcgccg gcatcccggt gagcgtctcg gcggccacgt gctacacgat ggacctcgcg
     1021 cgcccggcgg ccgaggtgaa cggcatggat gccgggcagt cgttccatct cgtgcacgtg
     1081 tacgacgaca cgatcaccca cgcggtggtt cccgtcgtcg atgccctcac gggcgagtac
     1141 ttctcagcgg agtgggtcga gcggatggcc gcgctcacgc ccgcggagcg gctcgaggcg
     1201 ttctcgcgca agcccggccg ataatcaccg gatcgattga cttggagcgc gctcgaagtt
     1261 ctaccgtggg gacatgaccc tcattcacga cggcgtcgcg atctcggaag ccgcctcggc
     1321 gaccggactc tcgaggcaca cgctgcgcta ctacgagcgt gctgggctca tgctcgaacc
     1381 ggtcgaccgc gcgtcttcga cgcaccggcg ctacagcgaa gccgacatcg gatgggtgca
     1441 gttcctcacc cggctgcgct ccaccggaat gccgatcgcc accatccgcg agtacacgga
     1501 tctcgtccgc cggggtgacg tcaccatcga cgcgcggcga gagctgctcg tgcgccaccg
     1561 catcgcggtg ctgcaccagc tcggagtcat ccaggcgagc ctcgccgcca tcgacatcaa
     1621 gatcgccatc tacgaagaga aagtcgcaca cgcatgaaga agatcaccct cggcaccgac
     1681 ggacccgtca tcggccgcat cggcctcggc ctcatgggca tgagcgcctt ctacaccggt
     1741 gccgccaaag acgatgccgg agccatccgc acgatccagc gcgcgctgga ggtgggcgtg
     1801 acattcctgg acaccgccga gatctacggc ccctacgtca atgaggagct cctcgggcgt
     1861 gccctcgggg ggcgccggga cgaggtcgtg atcgccacca agttcggcac gatccgccac
     1921 agcatcgacg acaagggcgg actcgacggc agtcccgcga acgtccggct ctcggtcgag
     1981 gggtcgctgc gccgcctcgg caccgaccac atcgacctgt actaccagca ccgcatggac
     2041 cccggcacgc ctatcgaaga gacggtcggc gccctcgccg agctcatcgc ggagggcaag
     2101 atccgccact acggcctgtc cgaggctgcg ccggcgacga tccggcgcgc gcacgccgtg
     2161 cacccggtca ccgcgctgca gaccgagtac tcgctgtgga gccgcgaccc cgaagcagag
     2221 atccttccga cggtgcgcga gctcggcatc gggttcgtgc cctattcgcc actgggtcga
     2281 gggttcctga ccggcgcgat ccgctccctg gatcagctgg atgagggcga cttccgcaag
     2341 tcgaacccgc ggttcgccga cggaaacctc gatgcgaacc tcgtgatcgt cgagaaagtc
     2401 gagtccgtcg cggccgagat cggagcgact ccggctcagg tggcgctggc ctggctgctc
     2461 gcccagggcg atgacatcgc cccgattccc ggcacacggc gcatcgccgc gctcgaggag
     2521 aacgtcggcg cggacgcggt ggtgctctcg cccgagcatc tcgctgcgct cgcctcgctg
     2581 gatgccgcag ccggcgacag gtacgccgac atgtcgccgt tgacccgctg aggcccgcca
     2641 gcggctcatc gccgccacgt acagcgcgga cgcgtgggtt gtccgcgctg tacgggtgtc
     2701 tcggcccacc gcgagagtaa cctcgaagca tcccttcaca tcgccgtgac cgacccacga
     2761 ggacacacac atggctctgg acgcaatgac gcgcacacgt atcgagaccg actccctggg
     2821 atccatggag atccccgccg acgcgtattg ggggatccac accgcccgcg cgctcgagaa
     2881 cttcccggtc tcgaaacggc cgatctcggt gtacaaggac ctggtccgcg cgctcgcgat
     2941 ggtcaagcag gcctccgccc gcgccaaccg cgacatcggc gcgctcgacg cgtacaaggc
     3001 cgacctgatc gaccgggcct cgcagctcgt gatcgacggt gagtaccacg atcagttcgt
     3061 cgtcggggtc atccagggcg gcgccggaac ctccacgaac atgaacgcga acgaggtcat
     3121 caccaacatc gcgctcgagc tcgcgggccg cgagaagggc gactacgact tcctgtctcc
     3181 gatcgacgac acgaaccgca gccagtccac gaacgacgtg taccccacgg cggtcaaggt
     3241 gggactcggc ctcgacctgc gcacgctgct ggacgagctg gacctgctgc ggcgttcgtt
     3301 cctggccaag gcggtcgagt tccacgacat cctgaaggtc ggccgcaccc agctgcagga
     3361 tgccgtcccg atgaccctcg ggcaggaatt caacggattc gccacgaccc tcggcgagga
     3421 ccacagccgg ctcaaggaga acgcctacct cctctgcgag atcaacatgg gcgcgacggc
     3481 gatcggcacc ggcatcacca cgcatccgga gtacggcaag tcggtcctgc gccacctgcg
     3541 cgagatctcc gggctcgacc tggacaccgc gacagacctg gtcgaatcga cgagcgacac
     3601 aggcgcgttc atgtcgttct cctcgacgct caagcgcaac gcgatcaagc tgtcgaagat
     3661 ctgcaatgac ctccggctgc tctcgtccgg accgcaggcc gggttcggcg agatcaacct
     3721 gcccgcccgg caggcggggt ccagcatcat gcccggcaag gtcaacccgg tgatccccga
     3781 ggtcgtcaac caggtcgcct tcgcggtcgt gggcgccgac atgacggtga cgatggcggt
     3841 cgaggcgggt cagctgcagc tgaacgcgtt cgagccggtc atcgcgcact cgctgttcca
     3901 gtcgatcacc tggatgcgcc ggggaatgca cacgctgcgg gtcaactgcg tcgacggcat
     3961 caccgccaac accgagcgcc tcggtgcgat ggtcggcgca tcggtcgggg tcatcacggc
     4021 gctcactccg ttcatcggct acgccgcggc tgccgccctc gccaagaccg ccctcctgac
     4081 cgggcgcaac gtcgcagacc tcgtggtcga agccggcctc atgtcacgcg aagagtgcat
     4141 gaagcagctg tcgccggccc gcctttcggg tctggagacc gtcacagcgg cgattccggt
     4201 cgtgtacgcc gacgattcgg gcgagacgga cgaatgaccg ggtggtgtgt cggtcgttgc
     4261 cgccggtcgc gcacgggcac tagcgttcga agcacagctt cc
//
LOCUS       DATDBZ010000454         2725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2610706, whole genome shotgun sequence.
ACCESSION   DATDBZ010000454 DATDBZ010000000
VERSION     DATDBZ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2610706"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VIX62_10775"
     CDS             <1..1231
                     /locus_tag="VIX62_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY5860760.1"
                     /translation="WPKEISYALVGSCTNSSYEDITRAASIAREAAARGLTCRTDLMI
                     TPGSEQVRATIERDGLLADFERIGATVLANACGPCIGQWSRSDVSEGDVNTIVTSYNR
                     NFPKRNDGLSTTFAFVTSPETVVALAIAGSLDFDPLTDTLTNDAGEEVRLSVPVGVEL
                     PDQGFTPGESGFIAPPADGSGVSVAVSPTSDRLQILEPFAPWNGQDFIGLPVLMKASG
                     KCTTDHISAAGPWLKYRGHLENISGNLFAGAVNAFTGAAGTAKDRTDDQTRPYPEIAK
                     RWGEAGTRWIAIGDENYGEGSSREHAAMEPRFRGAAAVITRSFARIHEANLKKQGVLA
                     FTFADPSDYDKIGEDDSISLLGLAELAPDVPVQGRIDHADGMTTDIELLHTMSPEQIE
                     WFRAGSALNIIRQNTAN"
     gene            complement(1248..2672)
                     /locus_tag="VIX62_10780"
     CDS             complement(1248..2672)
                     /locus_tag="VIX62_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY5860761.1"
                     /translation="MGRSAAIFDLDRTLLTGASGPVIGEHLRAGGLLAGRSIPGEGAL
                     YRIFDLVGENRPTMELTRRAARATAGWSREAVRSAGEAAAGVLFDRVPPFALAEIERH
                     RGEGRLLVMATTTPHDLIEPLAALLGFDAVIATRYQAVDDVYTGGIDGEFVWGRGKNR
                     AVAAWAVESDVDLSESHAYSDSYYDVPLLSAVGHPHVVNPDPRMLAYGLVKRWPIVHF
                     DVPPGVPKLVGLEAQQLVLTSLFPEMFPGVRFDIDAPDPLPSAGPGILVANHRSYFDP
                     VALAMALARHGRSVRFLAKRELFDAPVVGPIARALGGIPVDRGSGSDEPLRAAAGALA
                     AGEIVAVLPQGTIPRGPEFFEPELKGRWGAARLAAMTGAPIIPMALWGTERVWPRSSR
                     IPELWNVGNPPTVLVRAGAPFTLPKRSLKKQAAGPPSDAWLATATERIMAAIVELLPA
                     EARQPHEPTAEELARTYPAGHPPE"
BASE COUNT          440 a         1000 c          907 g          378 t
ORIGIN      
        1 ctggccgaag gagatcagct atgcgctggt gggctcgtgc accaactcct cctacgagga
       61 catcaccagg gccgcatcga tcgccaggga ggcagcggcg cggggtctca cctgtcgcac
      121 cgacctcatg atcacgccag gttccgagca ggtgcgcgcc accatcgagc gcgacggcct
      181 tctggccgac ttcgagcgca tcggcgccac cgttctggcc aacgcctgcg gcccgtgcat
      241 cggccagtgg agccgctcgg acgtgtccga gggcgacgtc aacaccatcg tcaccagcta
      301 caaccgcaac ttccccaagc gcaacgacgg cctctccacg accttcgcgt tcgtcacctc
      361 gcccgagacc gtggtcgcgc tggcgatcgc cggttcgctc gacttcgacc cgctcaccga
      421 cacgctcacc aacgacgccg gcgaggaggt ccgcctcagc gtccccgtcg gcgtggagct
      481 gcccgaccag ggattcactc ccggcgagag cggcttcatc gctccccccg cggacggctc
      541 gggcgtgtcc gtcgccgtca gccccacctc cgaccggctc cagatcctcg agccgttcgc
      601 tccgtggaac ggccaggact tcatcggtct cccggtcctg atgaaagcca gcggcaagtg
      661 caccaccgac cacatctccg cggcgggtcc gtggctgaag taccgcggcc acctggagaa
      721 catctccggc aacctcttcg ccggcgcagt caacgcgttc accggcgcgg ccggcaccgc
      781 gaaggaccgc accgacgacc agacccgccc gtaccccgag atcgccaagc gatggggaga
      841 ggccggcaca cgatggatcg ccatcggcga cgagaactac ggcgaaggct catcccgcga
      901 gcacgcggcg atggaacccc ggttccgtgg cgccgctgcg gtcatcaccc gatcgttcgc
      961 ccgcatccac gaggcgaacc tcaagaagca gggtgtgttg gcgttcacct tcgccgatcc
     1021 ctccgactac gacaagatcg gcgaggacga ctcgatctcg ctgctcggcc tggctgagct
     1081 ggcgcccgac gtccccgtcc aaggccgcat cgaccacgcc gacggcatga ccaccgacat
     1141 cgagctcctg cacacgatgt cgcccgagca gatcgagtgg ttccgagccg gcagcgccct
     1201 caacatcatc cgccagaaca ccgccaactg agcagcctgc tcgacgccta ctcgggaggg
     1261 tggccggccg ggtaggtgcg ggccagctcc tcggcggtcg gctcgtgggg ctgacgcgct
     1321 tctgccggga gcagctcgac gatcgccgcc atgatccgct cggtcgcggt ggccagccac
     1381 gcgtcactcg gaggcccggc agcctgcttc ttgagagacc gcttgggaag cgtgaacggt
     1441 gcgcccgcgc gcaccagaac cgtcggtggg ttccccacgt tccacaactc ggggatgcgc
     1501 gacgagcggg gccacacgcg ctcggttccc cagagggcca tcgggatgat gggtgcccct
     1561 gtcatcgcgg ccaggcgggc cgcgccccac cgtcccttga gctcgggttc gaagaactcg
     1621 ggtccccggg ggatcgtgcc ctgagggagc acggcgacga tctcacccgc cgcgagcgcg
     1681 cctgcagcgg cccgcagtgg ttcgtccgag ccgctgcccc tgtcgacggg tatcccaccc
     1741 agcgccctgg cgatcgggcc caccacggga gcgtcgaaca gctcgcgctt ggccaggaag
     1801 cgcacggatc gcccgtgccg ggcgagcgcc atggccaggg cgacggggtc gaagtacgag
     1861 cggtggttcg cgacgaggat gcctggaccg gcggagggga gagggtccgg cgcgtcgatg
     1921 tcgaatcgca ccccggggaa catctcgggg aagagcgacg tgaggaccag ctgctgggcc
     1981 tcgagcccca cgagcttggg cacgccgggc gggacgtcga agtggacgat cggccagcgc
     2041 ttcaccagcc cgtaggcgag catccgggga tcggggttca cgacgtgcgg gtggccgaca
     2101 gccgacagga gcggcacgtc gtagtagctg tcggagtagg cgtggctctc ggagagatcg
     2161 acgtcggact cgacagccca cgccgcgacc gcacggttct tgcctcgtcc ccacacgaac
     2221 tcgccatcga tgccgcccgt gtagacgtcg tccactgcct ggtatcgggt ggcgatgacg
     2281 gcgtcgaacc cgagcagagc cgcgagcggt tcgatgaggt cgtggggagt ggtggtcgcc
     2341 atgaccagca gccgaccctc gccacggtgg cgctcgatct ctgccagcgc gaacgggggg
     2401 acccggtcga agagcacgcc cgctgcggct tcccctgccg agcgcaccgc ctcgcggctc
     2461 catcccgcgg tcgcccgcgc cgcccggcgc gtcaactcca tcgtcggacg gttctcgccg
     2521 accaggtcga agatgcggta gagcgctccc tcgccgggga tcgagcgccc cgccagcagc
     2581 ccgcccgccc gcaggtgttc accgatcacg ggtccgctcg cgccggtgag cagcgtccga
     2641 tccagatcga agatcgctgc cgaccggccc atggcgcgca tgctaggacc gaccggcagc
     2701 ccacgtctcg gccgatgcag ctccc
//
LOCUS       DATDBZ010000455         2250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_775981, whole genome shotgun sequence.
ACCESSION   DATDBZ010000455 DATDBZ010000000
VERSION     DATDBZ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775981"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /gene="glyA"
                     /locus_tag="VIX62_10785"
     CDS             <1..1224
                     /gene="glyA"
                     /locus_tag="VIX62_10785"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY5860762.1"
                     /translation="SDPDIAAAIANETERQAHGLELIASENFVSSAVLEAAGSVMTNK
                     YAEGYPGRRYYGGCEFVDVTERLAIDRAKALFGADHANVQPHSGASANLAVFGAFLEP
                     HSDDKVLGMVLAHGGHLTHGSPVNVSGKWFNFVGYEVDRETEQLDMDRIRDLAKAERP
                     KIILSGYTAYPRTIDFAAFREIADEVGALLWVDASHFIGLVAGGAYPSPVPYADVVTF
                     TTHKALRGPRGAMIVCKEEHAKAIDKAVFPMMQGGPLEHCIAAKAVALKEASTPEFKA
                     YADRTVRDARALAAGLTDEGLRIVSGGTDSHLILADTRPLDIDGKTAEAVADEVGIAL
                     NKNQIPYDPNPPSTPSGIRVGTPGPSTLGMDEPEMREIAGIMGEVMRHPEDAGVKEKA
                     RAQVRDLMQRFPLYP"
     gene            1291..>2250
                     /locus_tag="VIX62_10790"
     CDS             1291..>2250
                     /locus_tag="VIX62_10790"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY5860763.1"
                     /translation="MSAYLLIFLASAGTTFLVTPLVRRMVVRMGAIDRPGDRKVHPVA
                     TPTMGGLAMYAGFLVGLGVSFLLPFFSDMQAGSPEPLAALVTCTLMVGLGLIDDRRGT
                     SALAKFTAQVFIAGVLYLMGASLTYFWIPGTLQVVSLTSDLSVLLTILWVVVIANAVN
                     LVDGLDGLAAGMVAITAGCFFIYVVRTPSTFGDASAAALLSVITVGICVGFLPWNFHP
                     AKIFMGDTGSMLLGMLVALFTISGVGRNPLPPSGGDLAAIAGPLLVLLLVLFIPFLDV
                     VLAVARRTWRGQGIGHADKEHIHHRLMEIGHSHPRAVLLMYLWS"
BASE COUNT          336 a          778 c          768 g          368 t
ORIGIN      
        1 tccgatccgg atatcgccgc ggcgatcgcc aacgagaccg aacggcaggc ccacggattg
       61 gaactgattg cctcggaaaa cttcgtcagc tccgcggtgc tcgaggccgc cgggtcggtg
      121 atgacgaaca agtacgccga gggctacccg gggcggcgct actacggcgg atgcgagttc
      181 gtcgacgtca ccgagcgact ggcgatcgat cgagccaagg cgctgttcgg ggccgaccac
      241 gcgaacgtgc agccccactc cggcgcctcg gccaacctgg cggtgttcgg tgcgttcctc
      301 gagccgcact ccgacgacaa ggtcctgggc atggtgctgg cgcacggcgg ccacctcacg
      361 cacggctcgc cggtgaacgt cagcggcaag tggttcaact tcgtcggcta cgaggtcgac
      421 cgcgagaccg agcagctgga tatggaccgc atccgcgacc tcgccaaggc cgagcgcccg
      481 aagatcatcc tgagcggcta cacggcctac ccccgcacga tcgacttcgc ggccttccgc
      541 gagatcgccg acgaggtcgg cgcactcctg tgggtcgatg cctcacactt catcggcctc
      601 gtggccggcg gcgcctaccc ctcgcccgtg ccctacgccg acgtggtcac cttcaccacg
      661 cacaaggccc tgcgcggccc gcggggcgcc atgatcgtgt gcaaggaaga gcatgccaag
      721 gcgatcgaca aggccgtgtt ccccatgatg cagggtggcc cgctggagca ctgcatcgcc
      781 gcgaaggccg tggcgctgaa ggaggcctcc acccccgagt tcaaggccta cgcggaccgc
      841 accgtgcggg atgcgcgggc cttggccgcc gggctcaccg acgaggggct ccgcatcgtg
      901 tccggcggca ccgattcgca cctgatcctg gccgacacgc ggccgctcga catcgacggc
      961 aagaccgccg aggccgtggc cgacgaggta gggatcgcgc tcaacaagaa ccagatcccc
     1021 tacgacccga acccgccctc gaccccgtcg gggatccgcg tcggtacgcc cgggccctcg
     1081 acgctcggca tggacgaacc cgagatgcgc gagatcgccg gcatcatggg cgaggtcatg
     1141 aggcaccccg aggacgcggg cgtcaaagag aaggcccgcg cccaggtgcg cgacctgatg
     1201 caacggttcc cgctctaccc atagcgggac agacccgcgt gcgggggctc ggttgccgca
     1261 cggcggccgg gcccgcacac tgagcccagc gtgagcgcct acctgctgat cttcctcgcg
     1321 agcgcgggga cgacgttcct cgtcacgccg ctcgtgcggc gcatggtcgt gcgcatgggg
     1381 gccatcgatc ggcccggcga tcggaaggtc catcccgtgg cgacgccgac catgggtggg
     1441 cttgcgatgt acgcgggctt cctcgtcggt ctcggtgtgt cgttcctgct gccgttcttc
     1501 tccgacatgc aggcgggcag cccggagcca ctcgccgcgc tcgtgacatg cacgctgatg
     1561 gtgggtctgg gcttgatcga cgatcggcgt ggcacctcgg cgctggcgaa gttcacggcg
     1621 caggtcttca tcgccggcgt gctgtacctg atgggtgcgt cgctgaccta cttctggatc
     1681 cccggaaccc tgcaggtcgt cagcctgacc tcggatctgt cggtcctact cacgatcctg
     1741 tgggtcgtgg tgatcgccaa cgccgtgaac ctggtcgacg gcctcgacgg cctggcggcg
     1801 ggcatggtgg ccatcaccgc cggatgcttc ttcatctacg tggtgcgcac gccgagcacg
     1861 ttcggggacg cctccgcggc ggccctgctg tcggtgatca ccgtggggat ctgcgtgggc
     1921 ttcctgccgt ggaacttcca tccggcgaag atcttcatgg gcgacaccgg ctcgatgctg
     1981 ctcggcatgc tcgtggcgct gttcacgatc tcgggggtgg gccggaaccc gctgccgccg
     2041 tcgggcgggg acctcgcggc gatcgcggga ccgctcctgg tgctcctgct ggtcttgttc
     2101 atcccgttcc tcgacgtggt gttggcggtg gcgcgccgca cctggcgggg tcaggggatc
     2161 ggccacgcgg acaaggagca catccaccat cgcttgatgg agatcgggca cagccaccct
     2221 cgcgcggtgc tgctgatgta cctgtggagc
//
LOCUS       DATDBZ010000456         5177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2611768, whole genome shotgun sequence.
ACCESSION   DATDBZ010000456 DATDBZ010000000
VERSION     DATDBZ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611768"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VIX62_10795"
     CDS             <1..243
                     /locus_tag="VIX62_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860764.1"
                     /translation="DKVVVYQGIPAKPLGLSLSSVDTITEIPAADAAALELYHDLPQG
                     MTADSREDALAIVEGIRTDVADDAVPPKKPKNASGQ"
     gene            240..1559
                     /locus_tag="VIX62_10800"
     CDS             240..1559
                     /locus_tag="VIX62_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY5860765.1"
                     /translation="MSEVAFAVDQRKRPVTGLGLMIVALVISIVAYATAGLGLKGKTP
                     RDLIAYGLVFAAVALAGWFAVRWLAPRADPVLYPIAVTLGGLGIAMLYRIMVDRGEAV
                     IAQEQAIWLIVGVAAFAATLWFVRDDRMLNTYTYTLGLIGVVLLLLPVVPGIGYEING
                     ARLWAAIGPITFQPAEFGKIFIVVFLAAYLADKRELLASGVGRFGLPRAKDLGPLLVA
                     WIASLAVLFLERDMGASLLFFGVFVVMLWIASGRAGYLWLGIILFVVGAWLGYLAFDH
                     VQTRVDYWIHALDPAVVTGQGYFQLAQGWFALATGGIVGTGIGQGSPTLIPYVGSDFI
                     FAAFGEELGMIGAVSLLLLYLVWIARGLRVAMERVDAFGKLLATGITTIIALQTFAIV
                     AGVTRLIPLTGVPLPLVSYGGSSRVATFIMLALLVRVSAGPRERQRG"
     gene            1552..3081
                     /locus_tag="VIX62_10805"
     CDS             1552..3081
                     /locus_tag="VIX62_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5860766.1"
                     /translation="MDRRIRRLGIGLVALFGLLFAQLAHVQVFAADDIKGQAANARRQ
                     IIAEYKVERGPILTAAPSSTVMAQSLQNEDERSELLFRRLYPDGELYADLTGYYSRIY
                     GRAGLEQAANPYLSGDAPELAISTFTDLILGRTKKGGAVVTTIDANLQAAAAQALGDQ
                     PGAVVAVDPRTGDVLALYANPSYDPNTLSNGTDQSIVDAWDAINADPDKPLVSRAKDE
                     LFLPGSTGKLITASAALENGFGPESEWDNPQFLDLPQSSNTLANFGNSLCNGGSAQVT
                     MAEAFKESCNVTFAEIGLKLGARNMSDQARAYGFCPTDPPDQIDCIEPTIPFVLPFQV
                     GHFPVPAYFQDNKPLLAFSSIGLDNVLTNPLHMALISAAIANGGKMMNPRLITEIRDA
                     SGRVVRTFESEQYGIPIGPQSAAELRAMMLSVTESGTAFSAFSGFPIQVAGKTGTATN
                     GEDVPPNAWFTAFAPAGVNDTPRIAVAVIVLDGGTLADESATGGAIAAPIAREVIDAY
                     L"
     gene            3097..4773
                     /gene="pknB"
                     /locus_tag="VIX62_10810"
     CDS             3097..4773
                     /gene="pknB"
                     /locus_tag="VIX62_10810"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEY5860767.1"
                     /translation="MSQSLLGGRYQMEARIGAGGMAEVFRGFDPVLNRTVAIKVLNPQ
                     FARDTSFVERFRREAQAAARLNQPNIVAVYDTGADEGTQFIVMEFIEGRTLGEFMESG
                     RKPTPVQAAEIAQKICAALAAAHAAGVIHRDIKPGNVMVTRDGTVKVMDFGIARVLGP
                     ETAPQTSAVLGTASYLSPEQAQGGPVDARTDIYSLGAVLYEMLAGRPPFMGDTPVAVA
                     YKQVNETPVVPSQLNPDVPARLDAVVMKALSKNPSNRYQTADEFSADLERVIKGQDVE
                     ATPLLAGVAAGEATQVISRPQQTAVLPPMEEPDGSGRKVWLGILIGILVVAVLAGGGY
                     LLVSSLTNDNEGSVLVVVPDLVGMTREQAEADLTDRGLNPVVKNRETDPADADPGTVV
                     DQNPKDGVEVAKGADVTIFIAVEPTTTTVPDLEGLTPSAAQTELSAAGLRLGSQLQEP
                     SATIPAGQITRSEPAAGSEIEQDSVVDVFVSTGPEQVTVPDVSTGCLSTGSARKALKD
                     AGLETEVGDPQPSVADCPNPSRIIGQEPAAGTTLDAGSVVTIFPGGGGDV"
     gene            4795..>5177
                     /locus_tag="VIX62_10815"
     CDS             4795..>5177
                     /locus_tag="VIX62_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY5860768.1"
                     /translation="MTLTFATEEFSPDERATLERHFTNLEGPVFALVNLPEAVKGALF
                     ARYSRTTKSLRRLFLDEFAEETAALGSAPAATVGSERAARLYENVFVEYGDDSVAQLG
                     GVHLACEQSSQLLAKALEWGRLAAYL"
BASE COUNT          818 a         1720 c         1809 g          830 t
ORIGIN      
        1 gacaaggtcg tcgtctacca gggcatcccg gccaaaccgt tgggtctgtc tctgtcgagc
       61 gtcgacacca tcaccgagat ccccgcagcc gatgccgccg ccctcgagct ctaccacgac
      121 ctcccgcagg ggatgaccgc cgacagtcgc gaggacgcgc tggcgatcgt cgaggggatc
      181 cggaccgacg tggcggatga cgcggtcccg ccgaagaagc cgaagaacgc gagcgggcag
      241 tgagcgaggt cgcgttcgcg gtcgatcagc ggaagcgccc cgtcaccggg ctgggcctga
      301 tgatcgtggc gctggtgatc tcgatcgtgg cctacgcgac ggcgggtctg ggcctcaagg
      361 gcaagacccc tcgcgatctg atcgcctacg ggctcgtgtt cgcggcggtc gcgctcgcgg
      421 ggtggttcgc cgtacggtgg ttggcgccgc gcgccgaccc cgtgctgtat ccgatcgcgg
      481 tcacgctggg cgggctgggc atcgcgatgc tctaccggat catggtcgac cggggcgaag
      541 cggtgatcgc ccaggagcag gcgatctggt tgatcgtcgg ggtggcggcc ttcgccgcga
      601 cgctctggtt cgtccgcgac gaccggatgt tgaacaccta cacgtacacg ctgggcctga
      661 tcggcgtggt gctgttgctg ctgccggtgg tcccgggcat cgggtacgag atcaacgggg
      721 cacggctgtg ggcggcgatc gggcccatca cgttccagcc ggcggagttc ggcaagatct
      781 tcatcgtcgt gttcctggcc gcctacctcg cggacaagcg cgaactcctc gcgagcgggg
      841 tgggacggtt cgggctaccg cgcgccaagg atctcgggcc gctgctcgtg gcgtggatcg
      901 cgtcgttggc cgtgctgttc ttggagcggg acatgggcgc gtcgctgctg ttcttcggcg
      961 tgttcgtggt gatgctgtgg atcgcgagcg gccgcgcagg ctacctttgg ctggggatca
     1021 tcctgttcgt ggtgggcgcg tggctgggct atctggcgtt cgaccacgtg cagacccgcg
     1081 tcgactactg gatccacgcc ctcgaccccg cggtggtgac gggccagggc tacttccagc
     1141 tggcgcaggg atggttcgcg ctggccaccg gcggcatcgt gggcaccggc atcggccagg
     1201 gttcgcccac gctgatcccg tacgtgggca gcgacttcat cttcgcggcg ttcggcgagg
     1261 aactgggcat gatcggcgcc gtctcgctgc tgttgctcta cctggtgtgg atcgcgcgtg
     1321 ggctgcgggt ggcgatggag cgcgtcgatg cgttcggcaa gctgctggcc accggcatca
     1381 ccacgatcat cgcgctgcag acgttcgcga tcgtggcggg ggtgacacgg ttgatcccac
     1441 tcaccggggt gcccctgccg ttggtgagct acgggggttc gagccgcgtc gccaccttca
     1501 tcatgcttgc gctgctggtg cgggtgtcgg cgggacctcg ggagcggcag cgtggataga
     1561 cgcatccgcc ggctgggcat cgggctcgtg gcactgttcg gactgctgtt cgcccaactc
     1621 gcacacgtcc aggtgttcgc ggccgacgac atcaagggac aggcggcgaa cgcccggcgc
     1681 cagatcatcg ccgagtacaa agtggagcgt gggccgatcc tgaccgcggc accgagcagc
     1741 acggtcatgg cccagagcct gcagaacgag gacgagcggt ccgagctcct gttccggcgg
     1801 ctctaccccg acggcgagct ctacgccgac ctcaccggct actactcgcg catctacggt
     1861 cgcgccggcc ttgagcaggc ggcgaacccc tacctgtccg gcgacgcgcc ggaactcgcg
     1921 atctcgacgt tcaccgacct gatcctgggc aggaccaaga agggtggcgc cgtggtcacc
     1981 acgatcgacg cgaacctcca ggcggccgcg gcccaggcgc tcggcgatca gcccggcgcg
     2041 gtggtggcgg tggatccccg caccggcgat gtgctggccc tgtacgcgaa cccctcctac
     2101 gaccccaaca cgctgtcgaa cgggacggac cagagcatcg tcgacgcgtg ggacgcgatc
     2161 aacgccgatc cggacaagcc cctggtctct cgagcgaagg acgagctgtt cctgccgggt
     2221 tccaccggca agctgatcac ggcctcggcg gcgctggaga acgggttcgg gccggagtcg
     2281 gaatgggaca acccccagtt cctcgacctg ccgcagagct ccaacacgct cgcgaacttc
     2341 ggcaactcgc tctgcaacgg gggttctgcc caggtgacga tggccgaagc attcaaagaa
     2401 tcgtgcaacg tcacgttcgc ggagatcggc ctgaagctgg gggccaggaa catgagtgac
     2461 caggcccggg cctacgggtt ctgccccacc gatccacccg accagatcga ctgcatcgag
     2521 cccacgatcc cgttcgtgct gcccttccag gtcgggcatt tccccgtccc ggcgtacttc
     2581 caggacaaca agccgctgct ggcgttcagc tcgatcggct tggacaacgt gctcacgaac
     2641 ccgctgcaca tggcgctgat ctcggcggcg atcgcgaacg gcggcaagat gatgaacccc
     2701 cgcctgatca ccgagatccg cgacgcgagc ggacgggtgg tgcgcacgtt cgaaagcgag
     2761 cagtacggga tccccatcgg accccagtcc gccgcggagt tgcgcgcgat gatgctgtcg
     2821 gtgacggagt cgggcacggc cttctcggca ttctcgggat tcccgatcca ggtggccggc
     2881 aagaccggca cggccaccaa cggcgaggat gtgccgccga acgcgtggtt cacggccttc
     2941 gcacctgcag gagtgaatga cacccctcgt atcgccgtgg cggttatcgt tctcgacggc
     3001 gggacgctcg ccgacgaatc ggcgaccgga ggcgcgatcg cggctccgat cgctcgagag
     3061 gtgatcgatg cgtatctgtg agatgagggg cacgggatga gccagtcgct gctgggtggt
     3121 cgctaccaga tggaggcgcg catcggcgcc gggggcatgg cggaggtgtt ccgcgggttc
     3181 gatcccgtgc tcaaccgcac cgtggccatc aaggtgctca acccgcagtt cgcgcgcgac
     3241 accagcttcg tggagcggtt ccggcgcgag gcgcaggccg ccgcccggct caaccagccc
     3301 aacatcgtcg ccgtctacga caccggcgcc gacgagggca cgcagttcat cgtgatggag
     3361 ttcatcgagg gccggaccct cggcgagttc atggagtccg gtcgaaagcc caccccggtg
     3421 caggcggcag aaatcgccca gaagatctgt gctgcgctgg cggcggcgca cgcggcaggc
     3481 gtgatccacc gcgacatcaa gcccgggaac gtcatggtga cacgcgacgg caccgtgaag
     3541 gtgatggact tcggcatcgc gcgcgtgctg ggacccgaga ccgcaccgca gacatctgcc
     3601 gtgcttggca ccgcgtcgta cctgtcgccc gaacaagcgc agggcggacc ggtcgacgcg
     3661 cgcaccgaca tctactccct gggtgccgtg ctgtacgaga tgctcgccgg ccggccgccg
     3721 ttcatgggcg atacccccgt ggcggtcgcc tacaagcagg tcaacgagac accggtcgtg
     3781 ccatcgcagt tgaaccccga cgtccctgcg cgcctcgatg cggtcgtgat gaaggcgctg
     3841 tcgaagaacc cgtcgaaccg ctatcagacc gccgacgagt tctccgccga cctggaacgt
     3901 gtcatcaagg gtcaggacgt ggaggcgacc ccgctgctgg cgggcgtcgc ggccggcgag
     3961 gccacccagg tgatcagccg tccgcagcag accgccgtcc tgccgcccat ggaagagccc
     4021 gacggttcgg gcaggaaggt ctggctgggg atcctcatcg ggatcctggt cgtcgccgtg
     4081 ctcgccggcg gtggctacct gctggtcagc agcctcacca acgacaacga gggcagcgtg
     4141 ctcgtcgtcg tgccggatct ggtgggcatg acccgggagc aggctgaggc cgacctcacc
     4201 gaccgcggcc tgaaccccgt cgtcaagaac cgcgagaccg atccggccga tgccgaccca
     4261 ggcaccgtgg tcgatcagaa tccgaaggac ggggtcgagg tcgccaaggg cgccgacgtc
     4321 acgatcttca tcgccgtgga gcccaccacc accaccgtgc ccgacctgga gggcttgacc
     4381 ccgtcggcgg cacaaaccga actcagcgcg gccggactcc gcctcgggtc ccagttgcag
     4441 gaaccgagcg ccacgatccc tgccgggcag atcacgcggt ccgagcccgc cgccgggagc
     4501 gagatcgaac aggattccgt ggtcgacgtg ttcgtgtcga ccggccctga gcaggtgacg
     4561 gtgccggacg tctcgaccgg ctgcctgtcg acggggtcgg cgcgcaaggc cctgaaggat
     4621 gccggcttgg agaccgaggt gggtgacccg cagccctcgg tcgccgactg tccgaacccg
     4681 agccgcatca tcggccagga accggctgcg ggaaccacgt tggatgccgg ttcggtggtg
     4741 acgatctttc cgggcggtgg gggcgacgtc tgagtccggg ggtaccctcg gaccatgacg
     4801 ctcaccttcg ccaccgagga gttctcaccg gacgagcgcg ccacgctcga acggcacttc
     4861 acgaacctcg aagggcccgt cttcgccctg gtgaacctgc ccgaagcggt gaagggcgcg
     4921 ctgttcgctc ggtactcgcg caccacgaag tcgctccggc ggttgttcct ggacgagttc
     4981 gcggaagaga ccgcggcgct ggggtcagcg ccggcggcca ccgtcggcag tgaacgcgcc
     5041 gcgcgcctct acgagaacgt gttcgtcgag tacggcgacg actcggtggc tcagctgggc
     5101 ggcgtgcacc tggcgtgtga gcagtcctcg cagttgctgg cgaaagcgtt ggagtgggga
     5161 cgcctggccg cctacct
//
LOCUS       DATDBZ010000457         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1161718, whole genome shotgun sequence.
ACCESSION   DATDBZ010000457 DATDBZ010000000
VERSION     DATDBZ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161718"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..919)
                     /locus_tag="VIX62_10820"
     CDS             complement(50..919)
                     /locus_tag="VIX62_10820"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY5860769.1"
                     /translation="MSESEVSVPLSVRLRFGHAAVQTLASTVGVDLLHIKGAAVDPSL
                     RPGGYAGTDVDVLVRPDHVAALDRALQQRGWRLYSTFEYGSPFGHAQTYRHDTWGYLD
                     IHRFFPGIRDDPGRAFDRFWADRHEVAIAGVGCPVPSVPAQAVLLVLNAARSPAGRRP
                     DVQLAWNEASPEHRAEIEALVADLDAPVAFAAATDGLDRYRGQRDYALWRVISEGGSR
                     ASEWWARVRAAPSLGAALRVAARAPLVNVDHFAHRLGRPPSRAEIVGEFFRRPGRAAR
                     EAWQGLRRAGANR"
     gene            1076..2212
                     /locus_tag="VIX62_10825"
     CDS             1076..2212
                     /locus_tag="VIX62_10825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY5860770.1"
                     /translation="MTTTASPTQRIQSVDGLRAVAFLAVFAFHTWEFAGRPDIPVLSS
                     VVSQNIRPDFFVVLTGFVLFLPFARRPERMDEFQTRSYLWRRMRRIVIPYYVALAYAV
                     LLPQLLVVIMRLLGREASWQPMPSLGDWLSHLTFSHLFFAEYWSSMNGSLWTMSLEMQ
                     LYLLFPLLLIAANRWGARALVVAIVLSVIFRIAVTVFVPGEAFPDQFLWSASGLGRLM
                     EFAAGMLAAIVAFKVVERIRRPHVFLLLGLMVVSYVIATAPVFAGTVLPIRELGLSAL
                     FGSLIVLVLSVPAIGRAFAWKPLSYLGYRSYSLFLIHQPTTWYVSEFLQKFLDVPEGP
                     LLLVLLWTVGFGVVFAVGQLMFVTVERPCIDWAKRARKPAPAQA"
     gene            complement(2290..>2505)
                     /locus_tag="VIX62_10830"
     CDS             complement(2290..>2505)
                     /locus_tag="VIX62_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860771.1"
                     /translation="ATIGRGGYLETVSPGVSGHHAASATAPDLAIAIRRARDLDPAAT
                     REWARGFGRDAHVAHLTRVMEEVVQRA"
BASE COUNT          356 a          861 c          835 g          453 t
ORIGIN      
        1 gtcccctcga cctcgagcgt gccgacgccg gcggccggac gcagccgggt catcgattcg
       61 ctccagcacg acgaaggccc tgccatgcct ctcgtgcggc acggcctggg cgacggaaga
      121 actcgccgac gatctcggcg cgcgagggcg gccgaccgag gcgatgggcg aagtggtcga
      181 cgttcacgag cggcgcacgc gccgctacac ggagtgcggc gccgaggctc ggggccgccc
      241 gcacccgcgc ccaccactcg ctcgcccggg agccaccctc cgaaatgacg cgccaaagcg
      301 cgtaatcgcg ttgaccgcgg tagcggtcaa gcccatcggt ggccgccgcg aaagccacgg
      361 gagcgtcgag gtcggcgacg agcgcctcga tctcggcgcg gtgttcgggg cttgcctcgt
      421 tccatgcgag ctgcacatcg ggccgcctgc cggccgggga ccgagcggcg ttgagcacga
      481 gcagcaccgc ttgcgcggga acgctcggca cggggcaccc gacgccggcg atcgcgacct
      541 cgtggcgatc ggcccagaac cgatcaaagg cccgacccgg atcgtcccga attccgggga
      601 agaagcggtg gatgtcgagg tacccccacg tgtcatggcg gtacgtctgg gcgtgaccga
      661 agggtgagcc gtactcgaag gtgctgtaga ggcgccagcc gcgctgctgc aacgctcgat
      721 cgagggcggc gacatgatcc gggcgaacga gcacgtcgac gtcggttccc gcgtagccac
      781 cgggcctcag cgatggatcc actgcggcgc ccttgatgtg gagcagatcc acgccgaccg
      841 tgctcgcgag ggtctgcact gcggcgtggc cgaaccgaag gcgcaccgaa agcggcacgc
      901 tgacctcgga ttcgctcacg cccgcttcac caccccgtcg atcactctag tgagttcgag
      961 acgcactcga tcccaccagt gtcacgcctc gctgcgccgc tgcgaagcgg tacacctgac
     1021 atctgccctg atctggggcc tacgtagggc gcccgcctca cctaggttgg tccctgtgac
     1081 caccacagcg agcccgaccc agcggatcca gtccgtcgac ggcctgcgcg cggtcgcgtt
     1141 cctcgcggtg ttcgcattcc acacctggga gttcgcgggg cgccccgata ttcccgtgct
     1201 gtcctcggtc gtcagccaga acatccgtcc cgacttcttc gtcgtgttga ccggtttcgt
     1261 gctcttcctc ccgttcgcga ggcgccccga acgaatggac gagttccaaa cacgctcgta
     1321 cttgtggcgt cgcatgcgtc gcatcgtgat cccctactac gtggccctgg cctatgccgt
     1381 gctcctgcca caactgctcg tggtgatcat gcggttgctc ggtcgtgagg cctcgtggca
     1441 gccgatgccg agcctgggcg actggctgag ccatctgacc ttctcgcacc tgttcttcgc
     1501 cgagtactgg agctcgatga acggcagcct gtggacgatg tcgctcgaga tgcagctcta
     1561 cctgttgttc ccgctgctcc tgatcgccgc aaaccgctgg ggggcgcggg cgctggtcgt
     1621 cgcgatcgtc ctctccgtga tcttccgtat cgcggtcact gtcttcgtgc ccggcgaagc
     1681 gttccccgac cagttcctgt ggagcgcctc gggtctcgga cgtctgatgg agttcgccgc
     1741 gggcatgctg gccgcgatcg tggccttcaa ggtggtcgag cggatccgtc ggccccacgt
     1801 cttcttgctg ctgggcctga tggtcgtgtc gtatgtcatc gcaacagccc ccgtcttcgc
     1861 cggtacggtc ctgccgatca gggaactcgg actgagcgcg ctgttcggaa gcctcatcgt
     1921 cctcgtgctg tccgtccccg cgataggtcg cgcgttcgcg tggaagccac tgtcatacct
     1981 cggctaccgg tcgtacagcc tgttcctcat ccaccagccg accacgtggt acgtctcgga
     2041 gttcctgcag aagttcctcg atgtgcccga agggccgctg ctgttggtcc tgctctggac
     2101 ggtcggcttc ggcgtggtct tcgccgtcgg gcagctcatg ttcgtgacgg ttgaacggcc
     2161 gtgcatcgac tgggcgaagc gcgcgcgcaa gcccgcgcct gctcaagcct gaatgctcgc
     2221 ggcggtggtc ggcgcagcgt cccggcggtg cgaaccggtg gtggattctc cgtcggatgc
     2281 agcggaggtt cacgcccgct gcaccacctc ttccatcact ctagtgaggt gggcgacatg
     2341 tgcgtcgcga ccgaatccgc gtgcccattc gcgagtcgcc gcagggtcga ggtcgcgggc
     2401 gcgccggatt gcgatcgcga ggtcgggagc ggtggcggat gccgcatggt gaccgctcac
     2461 tccggggctg acggtctcga ggtagccgcc ccggccgatc gtggc
//
LOCUS       DATDBZ010000458         2303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_978813, whole genome shotgun sequence.
ACCESSION   DATDBZ010000458 DATDBZ010000000
VERSION     DATDBZ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978813"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VIX62_10835"
     CDS             <1..216
                     /locus_tag="VIX62_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY5860772.1"
                     /translation="HLAHPEGSQRNMWACTVADVDQRADRVRVMLSGPVALVAEITPG
                     ALDALALQPGDPVWAAVKASEITTYPA"
     gene            252..1484
                     /locus_tag="VIX62_10840"
     CDS             252..1484
                     /locus_tag="VIX62_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5860773.1"
                     /translation="MARLEDLIDRFDVDDPAFIADPYPVLNALREAAPVFWNPRTNQW
                     ALTRFHDVSATLRDRRLGRSYSHLYTHGEVGRSEPDPRWAAFHRHERWSLLCLEPPDH
                     TRIRRLISKVFTPRAVAALRPSIEQFSAELLDRCAEQAEFDLLADYAQPYSVAVICSM
                     LGVPRADTDQLLAWSHAIVKMYELSTTDDVRAEANRAAAEYIEYTRALIADKRRSPDG
                     LLVSELVGVEDEGDVLTEDEIVSTTMVLVEAGHEATVNTLGNGVRALLKHPDEWWRLV
                     RGEVEPRTAVEEMLRWDAPLQLFERWILEPGVVIAGQQMEVGQEVAMLFGAAERDPRR
                     FDDPDRFDIGRGDPAHIGFGGGIHFCVGAPLARQEIEVSIGGIASRFPELALAAEPGY
                     HPTFVIRGLNALRLTPGR"
     gene            complement(1520..1834)
                     /locus_tag="VIX62_10845"
     CDS             complement(1520..1834)
                     /locus_tag="VIX62_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY5860774.1"
                     /translation="MSSNLANLEQRLHGELDQAVAQLQRLERQYEDVLADPGVLQEDR
                     DATRMLLEAARDVEAAAQRAVSRFEDGTYGRCVTCGNEIAPERLEALPDVDTCVTCAG
                     LA"
     gene            complement(2016..>2303)
                     /locus_tag="VIX62_10850"
     CDS             complement(2016..>2303)
                     /locus_tag="VIX62_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEY5860775.1"
                     /translation="VILATLAGVALTSVAQRHDMEQRNVNLQQAMLYREVIGQAQGIL
                     MEREHITADQAFAILRRASQHLNEKLRDVARTLVETGQVPDGGDEPRPAGA"
BASE COUNT          348 a          777 c          805 g          373 t
ORIGIN      
        1 cacctcgcgc atcccgaggg cagccagcgc aacatgtggg cctgcaccgt ggcggatgtg
       61 gatcagcgcg ccgaccgcgt cagggtcatg ctgagcggcc cggtcgcgtt ggtggcggag
      121 atcacccccg gcgcgctgga cgctctggcc ctgcaacccg gagatccggt ctgggcggcc
      181 gtgaaggcga gcgagatcac cacctacccg gcgtgatccg ccggtccgag gctcgacggt
      241 acggtggcag cgtggctcgg ctcgaggacc tgatcgatcg cttcgatgtc gacgacccgg
      301 cgttcatcgc cgatccgtac ccggtgctca acgcgctgcg cgaagcggca ccggtgttct
      361 ggaatcctcg gacgaaccaa tgggcgctga cccggttcca cgatgtctcg gcgacgctgc
      421 gcgaccggcg cctcggccgc agctactcac acctgtacac ccacggcgag gtgggccggt
      481 ccgagcccga cccgcggtgg gcggcgttcc accggcacga gcggtggtcg ctcctctgcc
      541 tcgaaccacc cgaccacacc cgcatccgac ggttgatctc gaaggtgttc acgccacgcg
      601 cggtcgccgc gctgcggccg tcgatcgaac agttctcggc cgagttgctc gatcgctgcg
      661 cggagcaggc ggagttcgat ctgctcgctg actacgcgca gccctactcg gtcgcggtga
      721 tctgctcgat gctcggcgtg ccacgcgccg acaccgatca actgctcgcc tggtcgcacg
      781 cgatcgtgaa gatgtacgag ctgtcgacga ccgacgacgt ccgggccgag gcgaaccggg
      841 cggcagccga gtacatcgaa tacacgcgtg cgctgatcgc cgacaagcgc cgctcgcccg
      901 acgggttact cgtttccgag ctcgtcggcg tcgaagatga gggcgacgtg ctcaccgagg
      961 acgagatcgt gtcgaccacg atggtgctcg tggaggcggg tcacgaggcg accgtgaaca
     1021 cgctcggcaa cggtgtgcgg gcgctgttga agcatcctga cgagtggtgg cgactcgtac
     1081 ggggcgaggt cgagccgcgg acggcagtcg aggagatgct gcgctgggat gcgcccctgc
     1141 agctgttcga gcggtggatc ctcgaacccg gcgtcgtgat cgccgggcag cagatggagg
     1201 tcggccagga ggtggcgatg ctgttcggcg cggcggagcg cgacccgcgg cgattcgacg
     1261 accccgaccg gttcgacatc ggccgcggcg atcccgccca catcggcttc ggcggcggca
     1321 tccacttctg cgtcggtgcc ccgctggccc gccaggagat cgaagtgtcc atcgggggga
     1381 tcgcaagccg gttcccggag ctggcgctcg ccgccgagcc cggctatcac ccgacgttcg
     1441 tgatccgcgg cctcaacgcc ctccggctga cgcccggccg ctgaacagcg gtcagcggcc
     1501 gaggatcagc agccgaggat caggccaagc ccgcgcacgt cacgcacgtg tccacgtcgg
     1561 gcagggcctc gagtcgctcc ggggcgatct cgtttccgca tgtcacgcac cgtccgtagg
     1621 tcccgtcctc gaaacggctc accgcccgtt gcgccgccgc ctccacgtca cgagccgcct
     1681 ccaggagcat gcgcgtggca tcgcggtcct cttgcaggac gcccggatcg gcgagaacgt
     1741 cctcgtactg acgctccagc cgctgcaact gggcaacggc ctggtcgagc tcaccgtgca
     1801 gacgctgttc gagattggcg aggttggagc tcatgggcac ctcgaggaga ggtcgtcgat
     1861 gtgtgtgcga cgagagggat ggaatggctg aatgcgacga ggaccgcttt gctcgaggct
     1921 cgatgtggat cgaaccccga ccgcacgcgg ccctcttcgg tccagcatac cgggcctacc
     1981 ggcggttcag cacgagggag cggccggcgg gtcggctacg cgccggccgg ccgcggttcg
     2041 tcgccgccgt caggtacctg tcccgtctcg acgagcgttc gggccacgtc gcggagcttc
     2101 tcgttgagat gttgcgaggc tctgcgcaga atggcgaacg cctggtcggc ggtgatgtgt
     2161 tcgcgctcca tgaggatccc ctgggcttgg ccgatcactt ctcgatagag catcgcctgt
     2221 tgcaggttga cgttgcgctg ctccatgtca tggcgctgag cgaccgacgt gagtgcgacg
     2281 ccggccagcg tggccaggat gac
//
LOCUS       DATDBZ010000459         6923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1373474, whole genome shotgun sequence.
ACCESSION   DATDBZ010000459 DATDBZ010000000
VERSION     DATDBZ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373474"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /gene="pyk"
                     /locus_tag="VIX62_10855"
     CDS             complement(<1..1188)
                     /gene="pyk"
                     /locus_tag="VIX62_10855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY5860776.1"
                     /translation="MTARTKLVCTLGPATNTPAFVRGLVAAGTSIFRINFSHGTPDDH
                     ARGVSLVRAAEAEGDRALAVLADLPGAKVRLGSVDPEPFRLAPGQSFALRPDGSGDEA
                     GTSTTYPGLAEDLRPGDRVLLADGAVELTVTAVLGSVVHTECVRGGTVRSGQGVNVPA
                     ERLGLPAITDRDREGLARALDLGVDVIAQSFVRGPEDIEQMRALMGERVVPIVAKIET
                     KPAIEHIEAILDVTDALMVARGDLGVELPMEQIPLLQKDLLRAARIAGRPVIVATQML
                     ESMIRAARPTRAEATDVANAVLDGADGIMLSGETAIGEYPFEAAAAATRIAEAVEARA
                     SEFRSGEPPCRHTGEAAAVAHAVADVASSEAEIVAITCYTETGRTARLLSAERPSCPI
                     YAFI"
     gene            complement(1185..2264)
                     /locus_tag="VIX62_10860"
     CDS             complement(1185..2264)
                     /locus_tag="VIX62_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEY5860777.1"
                     /translation="MSDIAGLVVAPAARLYDHGPAHPLRPERVLLTWDLLEAYGVTDT
                     DGVQRFDAEPASDATLLLVHTPEFLEATRRAGDGEDGPWGRFGYGPGDNPIFDRMHEA
                     GAIVVGATLAAAAAVLTGDVAHAFNAAGGLHHAMPSRASGFCVYDDPAVAIAWMLTQG
                     VDRVAYVDVDVHHGDGPQAIFYDDPRVLTISIHEFAPELGFFPGTGSPDEHGGPNAPG
                     SAINVPLAPGTDDAGWLAAFRDSVPAAVRDFGPDVLVTQLGCDTHASDPLAHLQLTTA
                     TYRRAAAILHELAHEAAGGRWVATGGGGYQWARVVPRAWTIAFAEMAEVELADPLPAT
                     WVAQATALSGGAVPATLSDPPGGLP"
     gene            complement(2261..3571)
                     /locus_tag="VIX62_10865"
     CDS             complement(2261..3571)
                     /locus_tag="VIX62_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5860778.1"
                     /translation="MTHVFPRVMDRDLPTAVHADGAWITTSDGRRFLDGAGGAIVVNV
                     GHGRRAVIEAVAAQLEATQYVHGTMFTTEAVEGYAEEVAVVLPMDGPRLYPVSGGSEA
                     VETALKLARAYHLANGEPSRFTMIGRRSSYHGNTIGALDASGKEPLRKPYTPWLGRYL
                     HAPAAYEYRCENPHHPDGCGAWHAGELERMIDQAPPKSIAAFIAEPVAGATLGAAVPC
                     EDYWPAMAEVCRRHGVLVIADEVMTGFGRTGRWFGVDHWQVRPDIMTAGKGTTGGYVP
                     FGYAAASAAVYDAVSTTGFVHGFTWSHNALGAAAGRAVLRLLHEEDLVARSAALGDRI
                     RHDLRSALADCPIVGDIRGLGTMIGIELVRDAATKEPFARTARVTERVVAHARDHELL
                     VYSSTGHVDGVDGDLIVLGPPFVLTDEDEAILVERTAAAIRSVA"
     gene            complement(3568..3798)
                     /locus_tag="VIX62_10870"
     CDS             complement(3568..3798)
                     /locus_tag="VIX62_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860779.1"
                     /translation="MTEPRDRPDPPEPWSKETAEEDEPDWAQSIRDGRKARGDRLRSV
                     FAGFDDADDDPTSGVPSRPAAPQDDRDPGVEP"
     gene            complement(3802..4740)
                     /locus_tag="VIX62_10875"
     CDS             complement(3802..4740)
                     /locus_tag="VIX62_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860780.1"
                     /translation="MDAIGRDEQDATREPFVDGLSWFAPLLAPTWLLPGVAKDAPRVE
                     LAAEPWPEEPAPDQDGRDLRQGLPLFLAEGIRYLTNALPSVRTEPVPHSGERAGAGIS
                     ILTSIVAGHPPVIRIRLMDAAFGLERDPIEREVESDADLGTVLVSLPRVTVDALRSTG
                     VRSSWDSRYSPPPPASAARVVRGHRACVRFADPAFYTAESADRRAAARDEARLVIRRL
                     ADAATSTSEALPAVLFFATLVGATRAGFDAANDFRMSVNARCMEATDPRDPVFRLSVI
                     ALRTIGDEAIAERRAHQLEAVEDPSFQDWLERARKA"
     gene            complement(4743..5528)
                     /locus_tag="VIX62_10880"
     CDS             complement(4743..5528)
                     /locus_tag="VIX62_10880"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004214463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY5860781.1"
                     /translation="MSTDVARDLGYSKSEIMIAASARQLAGVKNCFVGVGLPNIVCNL
                     SQRTVTPELQLVYESGVYGARPERLPLSIGDPTLATGSTAITSMFELFAFYLQAGLID
                     VAFLGGAQIDRHGNLNTTVIGDYANPKVRLPGSGGACEIAIHAKQILVIMRQAQRSFV
                     ERLDFRTSPGHSGDPAHDAARGWHGSGPTSVVTDLGTYAFDAATGEMTLVTLHPGVTL
                     DDVRANMGWEPRVAEELGETPPPTPEELRLIREELDPLGVYTK"
     gene            complement(5525..6442)
                     /locus_tag="VIX62_10885"
     CDS             complement(5525..6442)
                     /locus_tag="VIX62_10885"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY5860782.1"
                     /translation="MSKVTTMQDAVRDLVRDGDTVAIEGFTHLICFAAGHEIIRQRRR
                     DLTLARMTPDVVYDQMIGAGVASKLIFSWMGNPGVGNLHAVRRRIEKADPEPLEIEEY
                     SHFGMVCRYMAGAANLPFFPIRSYYESDIPTVNPKIVPMTSPYDPNDEVYVVPPLKPD
                     VAIVAAQRADAEGDTQIWGLLGCQKEVAFAARKVIVVVEEVVEESTIRQDPNRTVIPG
                     AVVDAVVEEPFSCHPSFTQGYYDRDNAFYLDWDRIAKDPATLEAWYDEWVYGLANHRE
                     YVEKFGDAYWDRLRPTPAPSGEVDYGRYA"
     gene            complement(6462..6845)
                     /locus_tag="VIX62_10890"
     CDS             complement(6462..6845)
                     /locus_tag="VIX62_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5860783.1"
                     /translation="MTSLVETLTFDSRDPEPVARFWCTALGYQVTDSDTQGVWIEDPA
                     GRGWPILFLVVPEDKSIKNRLHLDLSPPTTMAQEVERLVDAGATFVERVDEGGSYWTV
                     LTDPEGNEFCVLRGPDDDPPRQRPV"
BASE COUNT         1022 a         2458 c         2402 g         1041 t
ORIGIN      
        1 gatgaacgcg tagatcgggc acgacggccg ttcggcggac agcagccggg cggtgcgccc
       61 ggtctcggtg tagcaggtga tcgcgacgat ctcggcttcg ctcgaggcga cgtcggcgac
      121 cgcatgcgcg acggcggcag cttcaccggt gtgccggcac ggcggctccc ccgaccggaa
      181 ctccgacgcc cgcgcctcga cggcctcggc gatcctcgtg gccgcggcag ccgcttcgaa
      241 gggatactca ccgatcgcgg tctcgcccga gagcatgatc ccgtccgcgc cgtccagcac
      301 cgcgttggcg acgtcggtcg cttcggcgcg cgtcggccgg gcagcgcgga tcatcgactc
      361 cagcatctgg gtggccacga tcaccggccg gccggcgatg cgcgccgccc gcagcaggtc
      421 cttctgcagg agggggatct gctccatcgg cagctccacc ccgaggtcgc cgcgcgccac
      481 catcagggcg tcggtgacgt cgaggatggc ctcgatgtgc tcgatcgctg gcttcgtctc
      541 gatcttggcc acgatgggta cgacccgctc ccccatcagc gcacgcatct gctcgatgtc
      601 ctcggggccc cgcacgaacg actgcgcgat cacgtcgacc cccaggtcca gcgcgcgagc
      661 gaggccttcg cggtcgcggt ccgtgatcgc cgggagcccg agacgctcgg ccggcacgtt
      721 gacgccctgg ccggaccgca cggtgccgcc gcgcacgcat tcggtgtgca ccacggagcc
      781 caggacggcc gtcacggtca gttccaccgc cccgtcggcc aacaggacgc ggtcgccggg
      841 ccgcaggtcc tcggcgagtc ccggataggt cgtcgacgta cccgcctcgt cgccagagcc
      901 atccggtcgc agggcgaagg actgcccggg agcgagccgg aacggctcgg gatccaccga
      961 ccccagccgc accttggcgc ccgggaggtc ggcgaggacc gcgagcgccc ggtcaccctc
     1021 ggcttctgca gcgcgcacga gggacacccc gcgcgcatga tcgtcgggcg tcccatggga
     1081 gaagttgatg cggaagatcg aggtgccggc cgcaaccagg ccgcgcacga acgccggcgt
     1141 gttggtcgcc ggacccaggg tgcagaccag tttggtacga gcggtcacgg gagccctccc
     1201 ggggggtcgg acagcgtcgc ggggaccgcg ccgcccgaca acgccgtcgc ctgcgccacc
     1261 caggtggccg gcaaggggtc ggcgagttcg acctcggcca tctcggcgaa tgcgatcgtc
     1321 cacgcccgtg gcacgacgcg cgcccactgg tagcctccgc cgccggtcgc cacccatcgg
     1381 ccgccggccg cctcatgtgc aagttcgtgc aggatcgctg cggcgcgccg ataggtggcg
     1441 gtggtgagct gcagatgcgc gagcgggtcg gacgcatgcg tgtcgcagcc caactgggtc
     1501 acgagcacat cggggccgaa gtcgcgaacg gcggccggaa ccgagtcgcg gaaggccgcc
     1561 agccaaccgg catcgtccgt gccgggtgcc agcggcacgt tgatggctga tcccggggcg
     1621 ttcggaccgc catgttcgtc gggcgagccg gttccgggga agaacccgag ttcaggcgcg
     1681 aattcgtgga tcgagatcgt caggacgcga gggtcgtcgt agaagatcgc ctgcggaccg
     1741 tcgccgtggt gcacgtcgac gtcgacgtag gcgacccggt cgaccccctg ggtcaacatc
     1801 cacgcgatcg cgacggcggg gtcgtcgtag acgcagaagc ccgacgcccg ggacggcatc
     1861 gcgtggtgca gccctcccgc cgcgttgaac gcgtgcgcga cgtccccggt caggaccgcg
     1921 gcggccgcgg cgagggtggc ccccaccacg atggctcccg cctcgtgcat ccgatcgaag
     1981 atggggttgt cgccgggacc gtagccgaag cggccccatg gcccgtcctc gccgtcgccg
     2041 gcacgccgcg tggcctcgag gaactccggc gtgtgcacca acaggagggt cgcatccgag
     2101 gcaggctccg catcgaagcg ctgcaccccg tccgtgtcgg tcacgccgta ggcctctagc
     2161 aggtcccagg tgagcagcac gcgttcggga cgaagcgggt gggcggggcc gtggtcgtac
     2221 agccgtgctg caggggccac gaccagtcca gcgatgtcgc tcacgcgacc gaacgtatcg
     2281 cggcggcggt ccgttcgacg aggatcgcct cgtcctcgtc ggtgagcacg aagggcggcc
     2341 ccagcacgat gaggtcgccg tcgaccccgt cgacgtgacc ggtcgaggag tagaccagca
     2401 gctcgtggtc acgcgcgtgg gctacgaccc gctcggtgac ccgcgcggta cgagcgaacg
     2461 gctccttcgt cgcggcatcg cggacgagct cgatcccgat catcgtgccc agccctcgga
     2521 tgtcacccac gatcgggcag tcggcgagcg cggatcgcag gtcgtggcgg atccggtcac
     2581 ccagcgctgc gctgcgcgcc accaggtcct cctcgtgcag gagccggagc accgcccgtc
     2641 ccgccgccgc tcccaacgcg ttgtgcgacc acgtgaaccc gtggacgaac ccggtggtcg
     2701 acaccgcgtc atagaccgcc gcactcgccg ccgcgtagcc gaacggcacg taccccccgg
     2761 tggtgccctt gcccgccgtc atgatgtcgg gccgcacctg ccaatggtcg acgccgaacc
     2821 accggcccgt tcggccgaat ccggtcatca cctcgtcggc gatcaccaag acgccgtgcc
     2881 ggcgacagac ctcggccatc gccggccagt agtcctcgca gggaaccgcg gcgccgagcg
     2941 tggcgccggc caccggttcg gcgatgaagg ccgcgatcga cttcgggggg gcctgatcga
     3001 tcatgcgttc gagctcgccg gcgtgccagg cgccgcagcc gtcagggtgg tgggggttct
     3061 cgcagcgata ttcgtacgcc gcgggggcgt gcaggtagcg acccaaccac ggggtgtagg
     3121 gcttgcggag cggctccttg ccgctggcgt cgagcgcgcc gatcgtgttg ccgtgatagg
     3181 aactccgccg gccgatcatc gtgaaccgcg agggctctcc gttcgcgagg tgataggcgc
     3241 gcgccagctt cagcgcggtc tccaccgctt cgctcccgcc cgacacgggg tacaggcgcg
     3301 ggccatccat cggcaggacg accgccacct cctcggcgta tccctccacc gcctcggtcg
     3361 tgaacatcgt gccgtgcacg tactgcgtgg cctccaactg ggcggccacg gcctcgatca
     3421 ccgcccggcg tccgtggcca acgttgacca cgatcgcacc gcccgccccg tccaggaacc
     3481 ggcgcccgtc gctcgtcgtg atccacgcac catccgcatg cacggcggtc gggaggtcgc
     3541 ggtccatcac ccgggggaac acgtgcgtca cggttccacc cccggatcac ggtcgtcttg
     3601 aggagccgcc ggtcgcgacg gcacgccgct ggtcgggtcg tcatcggcgt cgtcgaatcc
     3661 ggcgaagacc gagcgcagcc ggtcaccacg ggctttgcgc ccgtcgcgga tcgactgtgc
     3721 ccagtcgggt tcgtcctcct ccgccgtttc cttcgaccac ggttcgggcg ggtcgggccg
     3781 gtcacgcggc tcagtcatcg ctcaggcctt cctcgctcgt tcgagccaat cctggaacga
     3841 cgggtcctcc acggcctcca gctggtgagc ccggcgttcg gcgatggcct cgtcgccgat
     3901 cgtgcgcaac gcgatgaccg acaggcggaa caccgggtca cgggggtcgg tggcctccat
     3961 gcatcgggcg ttcaccgaca tccggaagtc gttcgccgcg tcgaaccccg cgcgcgtggc
     4021 gcccaccagc gtggcgaaga acaagacggc gggcaacgcc tcgctcgtgc tggtggcggc
     4081 gtcggccagg cgccggatca ccagcctggc ttcatcgcgc gcggcggccc gacgatcggc
     4141 actctcggcg gtgtagaacg ccggatcggc gaaccggacg cacgcccggt gcccgcgaac
     4201 cacgcgcgcc gcagacgcgg gcgggggtgg cgagtaccgc gaatcccacg acgacctgac
     4261 gccggtggac cgcaacgcgt cgaccgtcac tcggggaagg gagacgagca cggtgccgag
     4321 atcggcatcg gactcgactt cccgttcgat cgggtcccgt tctagcccga aggccgcatc
     4381 catcagacgg atcctgatca cgggcggatg gcccgcaacg atcgacgtga ggatcgagat
     4441 cccggcgccg gcccgctcgc cggaatgggg taccggttcc gtacgaaccg acggcagggc
     4501 gttcgtcagg tagcggatcc cttcggcgag gaacaacggc aggccctgcc gcagatcgcg
     4561 cccgtcctgg tccggcgccg gttcctccgg ccatggctcg gctgccagtt ccacccgagg
     4621 ggcatctttg gcgacgccgg gcagcaacca tgtcggtgcg agcagcggtg cgaaccacga
     4681 gaggccatcg acgaacggct cccgtgtcgc gtcctgctcg tcccggccga tcgcgtccat
     4741 ggctacttgg tgtagacccc caggggatca agctcctcac ggatcaggcg gagctcctca
     4801 ggtgtgggcg gtggggtctc gcccagctcc tcggccaccc gaggctccca ccccatgttg
     4861 gcccggacat cgtccagcgt cacaccggga tggagggtga ccaaggtcat ctcccccgtg
     4921 gcggcgtcga acgcgtaggt gccgaggtcg gtgacgacgc tggtcggccc gctcccgtgc
     4981 catccgcgtg cggcgtcgtg cgcgggatcg ccgctgtgcc ccggcgaggt tcggaagtcc
     5041 agccgctcca cgaacgaacg ctgcgcctga cgcatgatca ccaggatctg cttcgcgtgg
     5101 atcgcgatct cgcacgcgcc gcccgagccc ggcaggcgga ccttggggtt ggcgtagtcg
     5161 ccgatcacgg tggtgttgag gttgccgtgg cgatcgatct gggctccccc caggaacgcg
     5221 acgtcgatca gccccgcctg cagatagaac gcgaacagct cgaacatgct ggtgatcgcg
     5281 gtggagcccg tggccagggt cgggtcgccg atcgagagcg gcagccgttc ggggcgggcg
     5341 ccgtacacgc ccgattcgta caccaactgc agctcagggg tcacggtgcg ctgggacagg
     5401 ttgcacacga tgttgggcag gccgacgccg acgaagcagt tcttcacgcc ggcgagctgg
     5461 cgggcgctcg cggcgatcat gatctcggac ttcgaatagc cgaggtcccg ggccacgtcg
     5521 gtgctcacgc gtacctcccg tagtcgacct cgcccgaggg cgccggtgtg ggccgcaggc
     5581 ggtcccagta ggcgtcaccg aacttctcga cgtactcgcg gtggttggcc agaccgtaga
     5641 cccactcgtc gtaccacgct tccagggtgg ccggatcctt cgcgatgcgg tcccagtcca
     5701 ggtagaaggc gttgtcgcgg tcgtagtagc cctgggtgaa cgacggatgg cacgagaacg
     5761 gttcctcgac cacggcatca accacggctc ctgggatcac cgtccggttg gggtcctggc
     5821 ggatcgtcga ttcctccacg acctcctcga ccacgacgat caccttgcgg gcggcgaacg
     5881 cgacctcctt ctggcagccg agcagccccc agatctgcgt gtcgccttcg gcatcggcgc
     5941 gctgggcggc cacgatcgcg acgtcgggct tgagcggcgg caccacgtag acctcgtcgt
     6001 tcggatcgta gggcgaggtc atcggcacga tcttcggatt caccgtgggg atgtccgatt
     6061 cgtagtagct acggatcggg aagaagggca ggttcgccgc gccggccatg tagcggcaca
     6121 ccatgccgaa atgcgagtac tcctcgatct cgagcggctc ggggtcggcc ttctcgatgc
     6181 gacggcgcac ggcgtggagg ttgcccaccc cggggttgcc catccagctg aagatcagtt
     6241 tgctcgcgac gcccgcaccg atcatctggt cgtagaccac gtcgggggtc atccgggcga
     6301 gcgtcaggtc gcgacgacgc tgacggatga tctcgtgtcc cgccgcgaag cagatcaggt
     6361 gggtgaagcc ctcgatcgcc acggtgtcgc cgtcccgcac gaggtcgcgc accgcgtcct
     6421 gcatggtcgt gaccttgctc atgcgacccc tgccgcgtgc ctcacaccgg tcgctgcctc
     6481 ggggggtcgt cgtccggacc gcgcagcacg cagaactcgt tgccctcggg gtcggtgagc
     6541 acggtccagt aactgccgcc ttcgtccacc cgttccacga acgtagcgcc ggcgtcgacc
     6601 agccgctcga cttcctgagc catcgtggtg ggtggtgaca ggtccaggtg gaggcggttc
     6661 ttgatcgact tgtcctcggg cacgaccagg aacaggatcg gccagcctcg cccggccggg
     6721 tcctcgatcc agacgccctg ggtgtcgctg tcggtcacct ggtagccgag cgccgtgcac
     6781 cagaaccgcg ccaccggctc tgggtcgcgg ctgtcgaacg tgagggtctc gacgagcgag
     6841 gtcacgcgcc cctacgagcc gccggcctcg acctcggcca ccgcgtcttc gaagatctcc
     6901 agcgcggctc gagcctgatc ctc
//
LOCUS       DATDBZ010000460         3070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_135473, whole genome shotgun sequence.
ACCESSION   DATDBZ010000460 DATDBZ010000000
VERSION     DATDBZ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135473"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1460
                     /locus_tag="VIX62_10895"
     CDS             165..1460
                     /locus_tag="VIX62_10895"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 4"
                     /protein_id="HEY5860784.1"
                     /translation="MEPRVVVIGGGSAYMPGVAFAFAHAGPTFAQATLVLQDIDSEAL
                     ELQGRLTANILRSRGASEVRVEIHRDRVRALEGADLVLAAFRPGGLAARHLDEQIAIE
                     HGVIGQETAGPGGFAMALRSVPVVVAIAEELHRVGNEGATLLDYTNPVQIVSEAVHRF
                     APRVPYVGLCDQTEGERRFLGRLMGCAPASIDLDTCGVNHMTFTQAIHIDGRDETVAV
                     WARLDSVEPDSLDAESERRIVRLFRMLGQIPSEYMQYFLFHDEVLAEQRTAGTTRAQE
                     VMALLPGVLASYREQADREHPQPSMARASEEHGDFAVSVMSAMRSTEPARFILNLPNR
                     GQVGDLPADAVVETPAMVHGGIVEPIDQGSLPPEVSGMVRQVVAHAQLAAEAAMTGSR
                     ALAVKALALHPLVRSVGTAEALVDAYLRAHAAHLPRFSA"
     gene            1460..2758
                     /locus_tag="VIX62_10900"
     CDS             1460..2758
                     /locus_tag="VIX62_10900"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY5860785.1"
                     /translation="MDATRSHPPLALDSVTSMLLDGRTKGMPHLAEPFELGRIGALEL
                     SVDDLPLPVMVLRASALEHNLALMAGLCRDHGVALAPHGKTTMAPQLWERQLAAGAWG
                     LTVATVAQARVMREVGVRRIVLANQLTDAPSIGWVASQLADPDLTFLTLVDSTHGVEL
                     LDEVLASVRSARRLPVLVEMGHMGGRTGCRTVADGVRVASAVHVSSHLELAGVESFEG
                     TVGADRSRATTQAVGSLLERVQTLAGEVGAIGSFTEERPMVVSAGGTAFFDLVLDHLA
                     GDRWGELPVNVVLRSGCYVTHDHGFYGSLTPAVPIEGGAQVFAPALELRGGVLSRPEP
                     DLAIVGFGKRDAPFDLGMPIPLGSRGRSGAVGDLGRDASVEHMNDQHAFLRIAKDVSL
                     GVGDVVRCGISHPCTAMDRWKVIPVIDDEERVVDAVATFF"
     gene            complement(2791..>3070)
                     /locus_tag="VIX62_10905"
     CDS             complement(2791..>3070)
                     /locus_tag="VIX62_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5860786.1"
                     /translation="PSEVAIKRGAQGAAVLDTDGVWTEHGGSVVQEVDPVGAGDAFNA
                     GYLHARLGGASAADALIAGADLGASVAGAFGDTPGSRHPSASGDGGQP"
BASE COUNT          437 a         1002 c         1102 g          529 t
ORIGIN      
        1 gacgtcggtc accgctgcca catagtcatc gtcgggcgcc ggccacgccg ggtaggcggt
       61 ggaccggacc ggggtccctc cactgatcgc gagttcactc atcgtcgtcg tgctccttcc
      121 gcgtgcggcg tgggtatcga ggtcctgctg ctagcgttcg cggcgtggaa ccgcgcgtgg
      181 tcgtgatcgg tggtggctcg gcatacatgc ccggtgtcgc gttcgcgttc gcccatgcag
      241 gcccaacgtt cgcacaggcg accctggtgc tgcaggacat cgattccgaa gccctggagt
      301 tgcaaggacg gctcaccgcc aacatcctgc ggtcccgagg tgcatccgag gtccgcgtcg
      361 agatccaccg ggaccgggtt cgtgcgctgg aaggagccga tctggtgctg gcggccttcc
      421 gtccgggtgg gctcgcggct cgtcacctgg acgagcagat cgcgatcgag cacggagtga
      481 tcggccagga gacggcgggt cccggcgggt tcgccatggc gctgcgttcc gtgcccgtcg
      541 tcgtggcgat cgcggaggaa ctgcatcgtg tcggcaacga aggggccacc ttgctcgact
      601 acacgaaccc ggtgcagatc gtgagcgagg cggtgcatcg attcgcgccc agggtgccgt
      661 acgtggggct gtgcgatcaa accgagggcg agcgtcggtt cctgggtcgg ctgatggggt
      721 gtgcgccggc ctcgatcgac ctcgacacat gcggggtgaa ccatatgacc ttcacccagg
      781 ccattcacat cgacggccgg gacgaaacgg tcgcggtgtg ggcccgcctg gactccgtcg
      841 aaccggactc gctcgatgca gagtccgagc gccgcatcgt tcggctgttc cgcatgctcg
      901 gtcagatccc cagcgagtac atgcagtact tcctgttcca cgacgaggtg ttggctgagc
      961 aacggacggc cggcaccacc cgagcccagg aggtgatggc cctgctgccc ggcgtcctcg
     1021 cgagctatcg cgagcaggcg gaccgagaac acccgcagcc gtccatggct cgtgccagcg
     1081 aggaacacgg ggacttcgcg gtgtcggtga tgtccgcgat gcgaagcacc gaacccgctc
     1141 gcttcatcct gaacctgccg aaccggggtc aggtgggcga tctgccggcg gacgccgtcg
     1201 tcgagacgcc cgcgatggtg cacgggggca tcgtcgagcc gatcgaccag gggagtctgc
     1261 ctcccgaggt gagcggcatg gtgcgccaag tggtcgcgca cgcacagctg gccgccgaag
     1321 cggcgatgac cgggagccgg gcgctcgccg tgaaggcgct cgcactgcat cccttggtcc
     1381 ggtcggtggg cacggcggag gcgctggtcg atgcgtacct gcgcgctcat gcggcgcatc
     1441 ttcctcggtt ctcggcgtga tggacgcgac acgttcgcac ccgccgctgg ccctggactc
     1501 ggtgacctcg atgctcctgg acggtcgcac gaaagggatg ccgcatctgg ccgagccgtt
     1561 cgagttgggc cggatcggag cgctcgagtt gtccgtggac gatctcccgc ttccggtcat
     1621 ggtcctgcgc gcgtcggcgc ttgagcacaa cctcgccctc atggctgggc tctgccgtga
     1681 ccacggcgtg gcactcgcgc ctcacgggaa gaccaccatg gcgccgcagt tgtgggaacg
     1741 ccagcttgcc gccggtgcgt ggggcctgac ggtcgccacg gtcgcacagg cccgggtgat
     1801 gcgtgaagtc ggggtccgcc ggatcgtgtt ggcgaaccag ctcaccgatg cgccatcgat
     1861 cggatgggtg gcgtcccagc tcgccgatcc ggatctcacc ttcctgacgt tggtggactc
     1921 cacgcacggg gtggagctgc tggatgaggt cttggcgtcg gtccgctccg cgcggcggct
     1981 tccggtgctg gtcgagatgg ggcacatggg tggtcggacc ggctgccgga cggtagccga
     2041 cggtgttcga gtcgcgagtg ccgtgcacgt ctcgtcgcac ctggagttgg cgggggtcga
     2101 gtccttcgaa ggaaccgtgg gagcagaccg ctcgcgagcc acgacccagg cagtgggctc
     2161 gctcctggaa cgggtgcaga ccctggcagg cgaggtcggt gccatcggtt cgttcaccga
     2221 agaacgtccc atggtggtgt cggccggtgg gaccgccttc ttcgacctgg tgctcgatca
     2281 tctggcgggc gatcgttggg gcgagttgcc ggtcaacgtg gtcctgcgat ccggctgcta
     2341 tgtcacgcac gatcatgggt tctacgggtc gctgacgccc gccgtcccca tcgagggggg
     2401 agcccaagta ttcgcgccgg cactcgagct gcgcggtggg gtcctctcgc ggcccgaacc
     2461 cgacctcgcg atcgtgggct tcggcaagcg cgacgctccg ttcgacctgg gcatgccgat
     2521 cccgctgggg tcgcgaggcc ggtcgggcgc ggtcggcgat ctgggtcgcg atgcgtcggt
     2581 cgaacacatg aacgaccagc acgccttcct ccggatcgcg aaggatgtct cgctcggggt
     2641 cggcgatgtg gtccggtgcg gcatctccca tccgtgcacc gccatggacc gctggaaggt
     2701 gatcccggtc atcgatgatg aggagcgtgt ggtcgatgcg gtggcgacct tcttctagcc
     2761 ggggatccgc tccggcatct ggccgccagc tcatggttgg cctccgtcgc cggacgccga
     2821 tggatggcgc gatccgggag tgtcaccgaa cgcaccggcg accgacgcac ccaggtcggc
     2881 acctgcgatg agtgcgtcgg cggccgaagc gccgcccaac cgcgcgtgca ggtaccccgc
     2941 gttgaacgcg tcgcccgcgc cgacggggtc gacctcctgc acgaccgaac caccgtgttc
     3001 ggtccagaca ccatcggtat cgagcacggc ggcgccctga gccccgcgct tgatcgcgac
     3061 ctcggaggga
//
LOCUS       DATDBZ010000461         4582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2615308, whole genome shotgun sequence.
ACCESSION   DATDBZ010000461 DATDBZ010000000
VERSION     DATDBZ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615308"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..573)
                     /locus_tag="VIX62_10910"
     CDS             complement(91..573)
                     /locus_tag="VIX62_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5860787.1"
                     /translation="MEGFTTHQACKFTGCTSRQLRYWDQIGLVRPTVQGTGGRPGVPR
                     LYSFRDLVALRVVKSLLDGGMSLQRVRRSWDFLNKKAGMDRHLSEVRLVTDGQSIFKI
                     CRRDGEVMDALREGQMAFFVAIDDIATGVETNVRQFEEDREKFVRALREAAAHTATGS
                     "
     gene            complement(764..1150)
                     /locus_tag="VIX62_10915"
     CDS             complement(764..1150)
                     /locus_tag="VIX62_10915"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEY5860788.1"
                     /translation="MRTRMPLSDHEQRILEEIERRLAEDDPKLDEQVGRTDLYTHLAR
                     RIRLACVGFVVGCLLLLLFVVSPWVAALGFVVMVLSALLLYRYLGQLGRDQIRAMQQG
                     GPLSMTGLIGRLAARFRPPPPPSSDE"
     gene            complement(1261..2442)
                     /gene="dinB"
                     /locus_tag="VIX62_10920"
     CDS             complement(1261..2442)
                     /gene="dinB"
                     /locus_tag="VIX62_10920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY5860789.1"
                     /translation="MTVFRPPEPMLHVDLDAFYASVESLKDPSLKGKPVIVGGVGGRG
                     VVSSTSYEARVFGVRSAMPTVRARRLCPDGVFVPPDFTAYQAHSNRFREVLLSFTPLV
                     EPISLDEAFLDVAGAERLFGPPETIAARIRADVEREVGVTCSVGVAPTKFVAKLASDG
                     CKPDGMLVVPAEGVLDYLEPLPVGRLWGVGEKTGDTLGRMGIRTIGDLSRTPQAILSR
                     LLGEQSATHLWQLSHGIDDRDVVPYEPPKSVGHEETFEHDLDDDEEILRELLALTGRV
                     ASRLRADGYRARTVTLKARLATFTTLTRSKTMADPTDVGADLYQTVAALYRALPGERR
                     RIRLLGVQATGLLSAGAEQLALLHGERWSDVERTIDRIEERFGRGAAQPASLLDRDRR
                     H"
     gene            complement(2468..>4582)
                     /locus_tag="VIX62_10925"
     CDS             complement(2468..>4582)
                     /locus_tag="VIX62_10925"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY5860790.1"
                     /translation="RREDVYDMILGRHGEARAGCVAMIDTYRARSAIREVGKALGLPE
                     VELGMIAKSFPHISASHLREAIDRLPELQGINLRFEQMELLFSVAERISGFPRHIAMH
                     PCGIVLASHDLVERVPLERSANGHRMIQADKDDVELLGYLKLDILGVRMLSSMRHALD
                     EIARTTGHKVDLDAIALDDEPTFDLIRRSDTLGCFQIESPGQRELLQKLQPSRWEDLI
                     VDISLFRPGPVKSDMIAPYLRRRAAMEAPVYVHPDLRPALQETHGVIVYHEQVIRTLA
                     AVAGYTETYADHIRRHLDHEEMLPGFREEFLRKATERGVDEEAAEKIWYAVAQFASFG
                     FCKAHAAAFAVPTYQSAWLKAHYPAHLIAGLLTHDPGMYPRRLILQDARRHDIPILPL
                     DVNRSEPEYVVEQVEEGFGIRLGLQDVHGISDAEIRSVLRARADRPFRDVGDFMRRTT
                     VSRPVTEALAHAGAFEAVGSAVGSGGSGGLKERASASVAPQQKAPQEGSRRRQLYRAM
                     TTEVEREGDQMALGFDGDPADGHTFRDYSDAEVVRAELEVMGMDTSRHIVSFFEPVLA
                     DLGVTRARDLRSVRAGTKVMVAGVKVASQTPAIRSGQRIIFLTLDDATGPVEVVVFES
                     VQPKVATTVFHGYALAITGELRRTGVAGLSVIAEDVWDLSALHRARAQGRLREAVGHD
                     EPGPVPGEGPRALWHASPGSAG"
BASE COUNT          721 a         1605 c         1547 g          709 t
ORIGIN      
        1 aggagctccg gaagctcacc gggttcggcc agcgcgtggg cggcctgtac cggcggacct
       61 gaccccgaag gagccgggct cgtcgccggg ctagctgccg gtggcggtgt gcgccgccgc
      121 ttcgcgaagg gcccgcacga acttctcgcg gtcctcttcg aactggcgga cgttggtctc
      181 gacgccggtc gcgatgtcgt cgatcgccac gaagaacgcc atctgccctt cgcggagcgc
      241 gtccatgacc tcgccgtcgc gccggcagat cttgaagatg ctctgcccgt cggtgacgag
      301 ccgcacctcg gacaggtgac ggtccatgcc cgccttcttg ttcaggaagt cccacgatcg
      361 tcggacgcgc tgcagactca tgccgccgtc gaggagggat ttcacgacgc gcagcgccac
      421 gaggtcgcgg aaggaataca gacgcggaac gccggggcgg ccgccggttc cttggacggt
      481 gggtctcacc aacccgatct ggtcccagta cctgagctga cgtgacgtgc agccggtgaa
      541 cttacaggcc tggtgggtag tgaatccctc catccggacg cgatccccct tcgtgtcttc
      601 acggttccac accggcactg ccctcgccga agggtcgacc tttgctcgac ggtgacgcgc
      661 ccaggggaag gggttctggt tgtacaccag cgcgcggaag agcggcaagc cgtgaggtca
      721 cgacagggaa caaatcaccc gaacgggcta cgagtctgcc ggctcactcg tccgacgacg
      781 gcggtggcgg cggacggaag cgcgccgcga gccgcccgat caaccccgtc atcgacagtg
      841 ggccgccttg ctgcatcgcg cggatctgat cgcggccgag ctgaccgagg taccgataga
      901 gcagcagcgc cgagagcacc atgacgacga agccgagcgc ggcgacccag ggactcacga
      961 cgaacagcag gaggaggagg caccccacca cgaacccgac gcacgccagc cggatgcggc
     1021 gagccaggtg cgtgtagaga tcggtgcgcc cgacctgttc gtcgagcttg ggatcgtcct
     1081 cggcgagacg tcgttcgatc tcctcgagga tccgttgctc gtgatcgctg agcggcatcc
     1141 gcgttctcac tcttcgcccg cccctcggtc cgggcggtcg gggtcgttga gttgattata
     1201 aggagcgctc ggtacaagga gaactccacc cgcgatgcaa ccttcgcgcc ggtgtgaccg
     1261 tcagtgacgc cggtcacggt ccaagagcga agccggttgg gccgcaccgc gtccgaaccg
     1321 ctcctcgatg cggtcgatcg tgcgctccac gtcggaccat cgctcgccgt gcaacagcgc
     1381 cagctgttcg gcgccggccg acaggagccc ggtggcctgc acgcccagca accggatgcg
     1441 ccgccgctcc cccggcaagg cgcggtagag cgcggcgacg gtctgataga ggtcggcgcc
     1501 gacgtcggtg gggtcggcca tcgtcttgga ccgggtgagc gtggtgaagg tggccaggcg
     1561 tgccttcagg gtcaccgtgc gggcccggta gccgtcggcg cgcaggcgtg aggccacgcg
     1621 tccggtgagc gccagcagct cgcgcaggat ctcctcgtcg tcatccaggt cgtgttcgaa
     1681 ggtctcctcg tggcccaccg acttcggcgg ctcgtacggc acgacgtcgc gatcgtcgat
     1741 gccgtgggag agctgccaca ggtgggtcgc cgactgctcc cccagcagcc gcgacaggat
     1801 ggcctgcggg gtccggctca gatcgccgat cgtgcggatg cccatgcgac ccagggtgtc
     1861 gccggtcttc tcccccacgc cccacaaccg gccgaccggg agcggctcga ggtagtcgag
     1921 cacgccctcg gcggggacga cgagcatgcc gtcgggcttg catccgtcgg aggcgagctt
     1981 ggccacgaac ttggtgggcg cgacccccac cgagcacgtg acccccacct cgcgctccac
     2041 gtccgcgcgg atcctggccg cgatcgtctc gggcgggccg aacaaccgct cggcgccggc
     2101 cacgtccagg aacgcctcgt cgagcgagat cggttccacc aggggcgtga acgacagcaa
     2161 cacctcgcgg aagcggttcg agtgcgcctg gtaggcggtg aagtcgggcg ggacgaacac
     2221 cccgtcggga cacagacgcc gggcgcggac cgtgggcatc gccgaacgca ccccgaacac
     2281 ccgcgcttcg taggaggtgc tggagacgac cccacgaccg cccaccccac ccacgatcac
     2341 cggtttgccc ttcagcgagg ggtccttcag cgattcgacg gaggcataga acgcgtccag
     2401 gtccacgtgc agcatgggct cgggaggtcg gaacacggtc atcgcggcgg acgcctcatg
     2461 gcgcccatca tcccgcggag ccgggactgg cgtgccagag cgccctggga ccctcccccg
     2521 gcaccgggcc gggttcgtcg tggccgaccg cttcgcgcag ccggccctgg gcccgcgccc
     2581 gatgcaacgc cgacaggtcc cagacgtcct cggcgatcac gctgagcccc gccacgcccg
     2641 tgcgccgcaa ctcgcccgtg atcgccaggg catacccgtg gaacacggtg gtggccacct
     2701 tcggctgcac cgactcgaac accaccacct ccaccggccc ggtggcatcg tcgagcgtca
     2761 ggaagatgat ccgctggccg ctgcggatcg ccggcgtctg cgaggccacc ttcaccccgg
     2821 ccaccatgac cttcgtaccc gcccggaccg atcgcaggtc gcgagcccgg gtcaccccaa
     2881 ggtcggccag caccggttcg aagaagctca cgatgtggcg cgaggtatcc atccccatca
     2941 cctcgagctc ggcccgcacc acttccgcat cggagtaatc cctgaacgtg tgcccgtccg
     3001 ccggatcccc gtcgaacccc aacgccatct gatccccttc ccgctccacc tcggtggtca
     3061 tggctctgta gagctgacgt ctcctgcttc cttcctgggg ggctttctgc tggggggcta
     3121 cgctcgctga cgctcgctcc ttgagccccc cagacccccc cgagccaaca gccgagccaa
     3181 cagcctcgaa agcgccggcg tgtgcgaggg cttcggtgac cgggcggctc accgtggtgc
     3241 gccgcatgaa gtcccccacg tctcggaacg gccggtcggc acgcgcccgc agcaccgacc
     3301 ggatctcggc gtcgctgatc ccgtgcacgt cctgcagccc cagccggatc ccgaaccctt
     3361 cctcgacctg ctcgaccacg tattcgggct cgctccggtt cacgtcgagc ggcaggatcg
     3421 ggatgtcgtg ccggcgagcg tcctgcagga tcaggcgcct ggggtacata ccgggatcgt
     3481 gggtcaggag gccggcgatc agatgggcgg ggtagtgcgc tttgagccag gcgctctggt
     3541 aggtgggcac cgcgaacgcg gcggcatggg ccttgcagaa cccgaagctg gcgaactggg
     3601 ccacggcgta ccagatcttc tcggcggcct cctcgtccac cccgcgttcg gtggccttgc
     3661 ggaggaactc ctcgcggaac cctggcagca tctcctcgtg atcgagatgg cgccggatgt
     3721 ggtcggcgta ggtctcggtg tagcccgcga cggcggccag cgtcctgatc acctgctcgt
     3781 ggtagacgat caccccatgc gtctcctgca gcgcaggacg cagatcggga tggacgtaga
     3841 ccggggcctc catcgccgcg cgccggcgca ggtagggcgc gatcatgtcc gatttcaccg
     3901 gtccgggacg gaacaacgag atgtcgacga tcaggtcctc ccagcgtgag ggctggagct
     3961 tctgcagcaa ctcgcgctgg ccgggcgatt cgatctggaa gcagcccagg gtgtcggagc
     4021 ggcggatcag gtcgaacgtg ggctcgtcgt cgagcgcgat cgcgtccagg tcgaccttgt
     4081 gcccggtggt gcgcgcgatc tcatcgagcg catggcgcat cgagctgagc atccgcacac
     4141 ccaggatgtc gagcttcagg taccccagca gctcgacgtc gtccttgtcg gcctggatca
     4201 tgcggtggcc gttggcgctg cgttccagcg gcacgcgttc gaccaggtcg tggctcgcca
     4261 gcacgatgcc gcacgggtgc atcgcgatgt gtcgggggaa cccggagatc cgctcggcca
     4321 ccgagaacag cagttccatc tgctcgaacc gcaggttgat cccctggagt tcgggcaggc
     4381 ggtcgatcgc ctcgcgcagg tggctcgccg agatgtgcgg gaacgacttc gcgatcatgc
     4441 ccagctcgac ttcgggcaga ccgagcgcct tgccaacctc gcggatcgcc gagcgcgcgc
     4501 ggtaggtgtc gatcatcgcg acgcacccgg cgcgcgcctc accgtggcga ccgaggatca
     4561 tgtcgtagac gtcttcgcgg cg
//
LOCUS       DATDBZ010000462         2140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2615715, whole genome shotgun sequence.
ACCESSION   DATDBZ010000462 DATDBZ010000000
VERSION     DATDBZ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2615715"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VIX62_10930"
     CDS             complement(<1..139)
                     /locus_tag="VIX62_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY5860791.1"
                     /translation="MATIYYEKDTDTGVLADQKIAVLGFGSQGHAHAQNLKDSGFDVR
                     VG"
     gene            complement(186..716)
                     /gene="ilvN"
                     /locus_tag="VIX62_10935"
     CDS             complement(186..716)
                     /gene="ilvN"
                     /locus_tag="VIX62_10935"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007821768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY5860792.1"
                     /translation="MTNHQALHTISVLVEDRPGVLTRVAGLFAARGYNIDSLAVGPTE
                     TPGLSRMTIVVNVEDNPLEQVTKQLNKLVNVIKILEHERGSAVERELMLAKIRAQGDV
                     RARIMEICEVFRVNIVDVTATTLTVEASGKPEKLEALRELLNEYGIVELSRTGRIALA
                     RGDRGIRERVLKSASA"
     gene            complement(713..>2140)
                     /gene="ilvB"
                     /locus_tag="VIX62_10940"
     CDS             complement(713..>2140)
                     /gene="ilvB"
                     /locus_tag="VIX62_10940"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEY5860793.1"
                     /translation="VGITRGVTKHNELVTRAEDIPLAIRQAFHIATTGRPGPVLVDIP
                     KDIVDPRNPASSMDWYWPSDDDVSSGLPGYKPTTKGHPRQIREAAELITAARRPVLYI
                     GGGILKARAAEALRELAELTGIPVVTTLMARGAFPDDHELCLGMPGMHGNYTAVTAMQ
                     RSDLLIALGSRFDDRVTGKLAGFAPHAKVIHVDIDPAEIGKNRAVDVPIVGDCRDVIG
                     KLHAELAKRQDEGDGLPDRSAWIDQLHAWQEEFPYKYDQQPDGALKPQYCVERLRAFA
                     DDDTIVVSGVGQHQMYASQFWGFEKPRHWINSGGLGTMGFAVPAAMGAKVGQPNKRVV
                     AIDGDGCFQMTAQELATSATENIPFVTAILNNGYLGMVRQWQELFFDERYSGVWLDPN
                     VPDYVKLAEAYGAVGLRVDSPGEVDAAIEKAYSVTDRSVVIDFRVDPSEMVFPMVPSG
                     ASNDDIIVGPEAITPTTDPAEAPIA"
BASE COUNT          303 a          693 c          757 g          387 t
ORIGIN      
        1 ggccgacccg cacgtcgaag ccggagtcct tcaggttctg cgcgtgcgcg tgaccctgac
       61 taccgaaccc gagcacggcg atcttctgat cggcgagcac gcccgtgtcg gtgtccttct
      121 cgtagtagat cgtcgccatg atggtccccg gtcccttccg tacggtcggt ctcgtcttcg
      181 gactgctagg cgctcgcgct cttgaggacg cgctcgcgga tgccacggtc accgcgcgcc
      241 aaggcgatgc ggccggtgcg cgagagctcc acgatgccgt actcgttgag gagctcgcgg
      301 agcgcttcga gcttctcggg cttgccgctg gcctccacgg tcagcgtggt ggcggtgaca
      361 tcgacgatgt tgacgcggaa gacctcgcag atctccatga tgcgtgcgcg gacgtcgccc
      421 tgggcgcgga tcttcgccag catgagctcg cgttcgacgg ccgagccgcg ctcgtgctcg
      481 aggatcttga tcacgttgac cagcttgttg agctgcttcg tgacctgttc cagcgggttg
      541 tcctcgacgt tgaccacgat ggtcatgcgt gacaggcctg gggtctcggt gggacccacg
      601 gccagcgaat cgatgttgta gccacgcgcg gcgaacagcc cggccacgcg ggtgagcacg
      661 ccgggccggt cctccaccag gaccgagatc gtatgcagtg cctggtggtt ggtcatgcga
      721 tcggtgcctc cgcggggtcg gtggtgggcg tgatggcctc gggtcccacg atgatgtcgt
      781 cgttcgaggc gcctgagggc accatgggga acaccatctc ggacgggtcc acccggaagt
      841 cgatcaccac gctgcgatcg gtgaccgagt aggccttctc gatggcggca tcgacctccc
      901 ccgggctgtc gacgcgcagg ccgacggcac cgtaggcctc ggcgagcttc acgtagtcgg
      961 gaacgttggg gtcgagccac acgcccgaat accgctcgtc gaagaacagc tcctgccact
     1021 ggcgcaccat gcccaggtaa ccgttgttca ggatcgccgt gacgaacggg atgttctcgg
     1081 tggcgctggt ggcgagctcc tgtgccgtca tctggaagca gccgtcgccg tcgatcgcga
     1141 ccacgcgttt gttcggctgg cccaccttgg cgcccatggc cgccggcacc gcgaagccca
     1201 tggtgcccag gccgccggag ttgatccagt gacgaggctt ctcgaagccc cagaactgcg
     1261 aggcgtacat ctggtgctgg cccacgccgc tcaccacgat cgtgtcgtcg tcggcgaacg
     1321 cgcgcaggcg ctcgacgcag tactgcggct tcaacgcgcc gtcgggctgc tggtcgtact
     1381 tgtaggggaa ctcctcctgc cacgcgtgca gttggtcgat ccaggccgaa cgatcgggca
     1441 gtccgtcgcc ctcgtcctgt cgcttggcca gctcggcgtg gagcttgccg atcacgtcgc
     1501 ggcagtcacc cacgatcggc acgtcgaccg cccggttctt gccgatctcg gcgggatcga
     1561 tgtcgacgtg gatcaccttc gcgtgcggag cgaatccggc cagcttgccg gtgacccggt
     1621 cgtcgaagcg ggagcccagc gcgatcaaca ggtccgatcg ctgcatcgca gtgaccgcgg
     1681 tgtagttgcc gtgcatgccg ggcatgccca ggcagagctc gtggtcgtcg gggaacgcgc
     1741 cgcgggccat cagcgtggtg accaccggga tgccggtcag ctcggccagc tcgcgcagtg
     1801 cctcggcggc gcgggccttg aggatcccgc ccccgatgta gagcaccggt cggcgagcgg
     1861 ccgtgatgag ctcggcggcc tcgcggatct ggcgcggatg acctttggtg gtcggcttgt
     1921 agccggggag gcctgacgac acgtcgtcgt cggagggcca gtaccagtcc atcgacgacg
     1981 cggggttgcg cgggtcgacg atgtccttgg ggatgtcgac gagcaccggg cccgggcggc
     2041 cggtcgtggc gatgtggaag gcctgccgga tggcgagggg gatgtcctcg gcgcgggtga
     2101 ccagctcgtt gtgcttggtg acgccgcggg tgatgccgac
//
LOCUS       DATDBZ010000463         2364 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_780935, whole genome shotgun sequence.
ACCESSION   DATDBZ010000463 DATDBZ010000000
VERSION     DATDBZ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780935"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VIX62_10945"
     CDS             complement(<1..181)
                     /locus_tag="VIX62_10945"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5860794.1"
                     /translation="MRTTDGAERVVTILDLLGSTPDGMGVSAIAEELGVHRSTTSRLM
                     GTLAASGIVERDAATR"
     gene            291..1253
                     /locus_tag="VIX62_10950"
     CDS             291..1253
                     /locus_tag="VIX62_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860795.1"
                     /translation="MTEGNPFDVDGVWLKSAFHTHTARSDGELDPAAHVRHHEWMGFD
                     VVAITDHWTLTHEPSTDHILVITGAELAADPYGEALADSEILAIGISDIPEDPGGDRA
                     KWGPIDAYTYKTFPDLSAAARCIADQGGVSFIAHPYWSGMPLETLMAVEGAHGIELFN
                     SSAQRENARGDSSYVWDLCLDRGKRFWAFGTDDCHYPGFDIGDAWTMVRAAERTEAAV
                     LEALRHGHTYASAGPQIRDITVDGTELEVRCSPARAVVMASRYETGWAVLAGARNRME
                     EARVLERDDRGLIVRARFSPSIELPHRRVVIEDEAGRKAWSNPI"
     gene            1235..2017
                     /locus_tag="VIX62_10955"
     CDS             1235..2017
                     /locus_tag="VIX62_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5860796.1"
                     /translation="MVQPDLSADLALAHELADVAAEVTLAWFGDRLPVELKADATPVT
                     KVDRAAERAIRSAIAARDPAAGVLGEEEGFDEGANGRRWVIDPVDGTKLYAEGIPLWT
                     TLIGLEIDGEAVLGVADAPALGQRYHAWRGGGAWRGKHRLRVSDVGTLDEAFVAHSGV
                     EEWSRGGHLDRLVAVVDRARGTRGLSDAWGHLLVAQGSVDALLEHEPCHEWDWTATGV
                     IVREAGGQISTLLGASPAAGDDLLVSNGVMHTPVIDVMAARR"
     gene            2109..>2364
                     /locus_tag="VIX62_10960"
     CDS             2109..>2364
                     /locus_tag="VIX62_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860797.1"
                     /translation="MRSQAPKSGTRAAGVLLAAMTAAVLVAPLARAQSSSPSGGEDLV
                     LRVGTTSDLITDNPWAVSAGSDWSVVTIQYDMLLKFASKDL"
BASE COUNT          363 a          762 c          880 g          359 t
ORIGIN      
        1 ggcgggtcgc ggcgtcgcgc tcgacgatgc cgctggcggc cagggtcccc atgaggcgcg
       61 acgtcgttga gcgatgcacg ccgagctcct ccgcgatcgc cgagacgccc atcccgtccg
      121 gcgtcgagcc gagcaggtcg aggatcgtga cgacccgttc tgccccgtcc gtcgtgcgca
      181 cgtcgggagc ctacgtcgtt gttgccgtca cggcacgtgc caccttgtgt cgtgaccgcc
      241 acggctctag cgtccgcgcg acgacccacc gacgaggaga cgcgatcgcg atgaccgagg
      301 ggaacccgtt cgacgtcgac ggggtgtggc tcaagagcgc gttccacacc catacggcgc
      361 gctccgacgg cgaactcgac ccggcggcgc acgtccggca tcacgagtgg atggggttcg
      421 atgtcgtggc catcaccgac cactggacgc tcacgcacga accctcgacc gatcacatcc
      481 tggtgatcac cggcgccgaa ctcgccgccg acccctacgg cgaggcgctc gccgacagcg
      541 agatcctggc gatcgggatc agcgacatcc ctgaggaccc cggtggtgac cgagcgaagt
      601 ggggtccgat cgatgcgtat acctacaaga cgttccccga tctgtcggcg gcggctaggt
      661 gcatcgccga ccagggtggg gtctcgttca tcgcgcatcc gtattggagc gggatgccgc
      721 tggaaaccct gatggcggtc gagggtgcgc acggcatcga actgttcaac tccagcgcgc
      781 aacgcgagaa cgcgcgcgga gactcgtcgt acgtctggga tctctgcctg gaccgcggca
      841 agcggttctg ggcgttcggg accgacgact gtcactaccc ggggttcgac atcggtgatg
      901 cctggaccat ggtgcgtgcg gctgagcgca ccgaggcggc ggtgctcgag gcgctgcggc
      961 acggacacac ctacgcgagc gccgggccgc agatccggga tatcaccgtg gacggcaccg
     1021 aactcgaggt ccgctgcagc ccggctcgag cggtggtcat ggcgtcgcgc tacgagaccg
     1081 ggtgggcggt cttggcgggc gcgcggaacc ggatggaaga ggccagggtg ctggagcgcg
     1141 acgaccgagg gctcatcgtg cgcgcacggt tctcaccgtc catcgagctg ccgcatcggc
     1201 gggtcgtcat cgaggatgaa gcggggcgca aggcgtggtc caacccgatc tgagcgccga
     1261 cctcgcgttg gcgcatgagc tcgcggatgt cgccgccgag gtgacgctgg cgtggttcgg
     1321 ggaccggctc ccggtcgagc tgaaggccga tgcgacgccg gtgaccaagg tggaccgggc
     1381 cgccgaacgc gcgatccgct cggcgatcgc cgctcgtgac cccgctgcgg gcgtcctcgg
     1441 ggaggaggaa gggttcgacg aaggcgccaa cggccgtcgg tgggtgatcg atccggtcga
     1501 cggcaccaag ctgtacgcgg agggcatccc gctgtggacg acgctgatcg ggctcgagat
     1561 cgacggcgag gccgtcctgg gggtggccga tgcacccgcg ctcggccagc ggtaccacgc
     1621 gtggcgcgga ggtggtgcgt ggagggggaa gcatcggctc cgcgtctcgg acgtcggcac
     1681 gctcgacgag gccttcgtgg cccattcggg ggtggaggaa tggagccgcg gcggccacct
     1741 cgatcgattg gtggccgtcg tcgatcgtgc gcgaggaacc agggggctga gcgacgcgtg
     1801 gggccatctg ttggtcgccc aggggtccgt cgacgcgctg ctcgagcacg agccttgtca
     1861 cgagtgggat tggaccgcca cgggtgtcat cgtgcgcgag gcgggcgggc agatctccac
     1921 gctgctcggc gcgtccccgg cagccggcga cgacctcttg gtgtccaacg gcgtgatgca
     1981 cacacccgtg atcgacgtca tggcggcgcg acgatgaacg caccgccccg ttgtcttgac
     2041 ggcacgtcgg tggttgttcg agggtcgatg cggacgacgt ccgcacggga accagggaag
     2101 ggaacgcgat gaggtcgcag gcaccgaagt ctgggactcg ggcggcaggc gtattgctcg
     2161 ccgcgatgac cgcggcggtc ctggtggcgc cgctcgcccg cgcgcagtcg tcgtctccga
     2221 gcgggggcga ggacctggtg ctccgggtcg ggaccaccag cgacctcatc accgacaacc
     2281 cgtgggccgt gagcgccggc agcgactggt cggtcgtcac gatccagtac gacatgttgc
     2341 tgaagttcgc ttccaaggat ctgg
//
LOCUS       DATDBZ010000464         3145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1799127, whole genome shotgun sequence.
ACCESSION   DATDBZ010000464 DATDBZ010000000
VERSION     DATDBZ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799127"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VIX62_10965"
     CDS             complement(<1..1584)
                     /locus_tag="VIX62_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5860798.1"
                     /translation="MVTCPSCSAANDPAARFCSSCGSALVRTCPSCGSLVPAQARFCP
                     ECGTSLDVASTPATVPVEERRVLSILFADLAGFTQRSDHADPEDVRGILVPFHAIAKE
                     EIERFGGVLDKFIGDAALGVFGAPIAHEDDAERAVRAALAIRERVSLEGMPVRVAVNT
                     GEALVTSGEGPQVGEHVAGDVVNTASRLQNLAPVGDVVVGETTARATRRVIDYEEMPP
                     VTVKGKSEPLGLWLARSVRPTVPERDEDDPPPFVGRERERQGLRDLLERTIRDRTPHL
                     VTVVGEPGLGKSRLLADLGEHVRAGSVDVAFHRGRCLPYGESITYAALVEIVRALADV
                     APDANRERAAEALDAYLPTLGTTADEGEWLSTRIHALVGPVAGSDQVVDRAESFAAWT
                     RFLELSAQRTPVVMVVEDLHWAEPAMLEFLDHVVEQAGSEPLLVLCTARPELFTMNPS
                     WGTGIARATTLTLSPLSDREMQELLGALLLRSVVPDDARGSLMRRAGGNPLYAREFVH
                     MLEDRAPALDGEGGRDIHDD"
     gene            complement(1595..1975)
                     /locus_tag="VIX62_10970"
     CDS             complement(1595..1975)
                     /locus_tag="VIX62_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEY5860799.1"
                     /translation="MQEPLSFWGGFDPQSGMVTDIHHPQLGVCLTGCVMFMTRGRGSS
                     SSSSVLAEAIHLGTAPAAIVMTETDPMIALGSVIAAELYGIEMPVVVMAPDTASRIVD
                     EIQVTVDATGAVAVLEAGPRMTRR"
     gene            complement(2032..>3145)
                     /locus_tag="VIX62_10975"
     CDS             complement(2032..>3145)
                     /locus_tag="VIX62_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEY5860800.1"
                     /translation="GMAGLDFARRLRDGAAQVAVPSTLNVSSLDLLHPELVRLDNTTS
                     TLAREQMDAYVAMGCRPTWTCAPYHSPVRPGFGDQIAWAESNAIVFANSVLGARTERY
                     SDFIDICCAITGRAPAAGLHLDEHRRAGVVFRVEGVPERLLTDPVAHAAIGLLVGREA
                     GALIPTVVGLPPAPTTSEDHLKAMCAAAATSGSVALIHVVGVTPEAPTLEASIGDGSA
                     AREVRVGPEDLRAARDALSTVGVGATVGAVSVGTPHLSVAGFERLASLVDGRRFAVPF
                     YVNTGRDVLRVAEEAGTVAVAEAAGATVVTDTCTYITPVIAAGVGPVVTDSAKWAWYA
                     PSNLGVEVAIASLQECIRSAVEGRLVRDDALWGAGE"
BASE COUNT          517 a         1096 c         1057 g          475 t
ORIGIN      
        1 atcatcgtgg atgtctcggc cgccttcccc atcgagggca ggtgcgcgat cctcgagcat
       61 gtgcacgaac tcacgtgcgt acagcgggtt gccgccggcg cggcgcatca gcgagcctcg
      121 ggcgtcgtcg ggaacgacgg atcgcaacag gagcgccccg agcagctcct gcatctcccg
      181 atcggacaat ggcgagagtg tcagggtcgt ggcgcgagcg atcccggttc cccatgaggg
      241 gttcatcgtg aacagctcgg gccgagcggt gcagagcacc agtaggggct cagatcccgc
      301 ttgctcgacg acgtggtcga ggaactcgag catggccggt tcggcccaat ggaggtcctc
      361 caccaccatc accactgggg ttcgctgtgc ggacagttcg aggaacctcg tccatgccgc
      421 gaaggattcg gcccgatcga ccacctggtc cgaccccgcg accggaccta ccaacgcgtg
      481 gatacgcgtg gagagccatt caccctcgtc ggcggtcgtg cccaatgtcg ggaggtaggc
      541 gtcgagtgct tcagcagcac gttctcggtt cgcatccggt gccacgtccg cgagcgcccg
      601 gacgatctcg acgagcgcgg cgtaggtgat gctctcgccg tacggcaggc accggccgcg
      661 gtggaacgcg acgtcgacgg agccggcacg aacgtgctcg ccgaggtcgg cgagcaaccg
      721 gctcttgccg agtcccggct ccccaaccac ggtgaccaag tgaggggtgc gatcgcggat
      781 cgtgcgctcc agcagatctc gcagtccttg ccgttcgcgt tcgcgcccca cgaacggtgg
      841 cgggtcgtct tcgtcgcgtt cggggaccgt ggggcgcacc gagcgggcga gccacagccc
      901 gagcggttcg gacttgccct tgacggtgac gggcggcatc tcctcgtagt cgatgactcg
      961 gcgggtggcg cgcgccgtcg tctcgcccac caccacgtcg ccgacgggag ccaggttctg
     1021 caggcgtgac gcggtgttca ccacgtcacc cgcgacgtgc tcgccgacct ggggtccctc
     1081 cccggacgtc accaatgcct cacccgtgtt caccgcgacg cgaacgggca tgccctcgag
     1141 cgagacacgt tcacggatgg cgagggcagc gcgtaccgca cgctctgcgt cgtcctcgtg
     1201 cgcgatcgga gcaccgaaca cccccaacgc cgcgtcaccg atgaacttgt cgagcacgcc
     1261 accgaaccgc tcgatctctt ccttggcgat cgcgtggaag gggaccagga tcccgcgcac
     1321 gtcctcgggg tccgcgtgat cggaacgctg cgtgaacccc gcgaggtcgg cgaacaggat
     1381 cgacaacaca cgcctttcct cgaccggcac cgtggcgggc gtgcttgcca cgtcgagcga
     1441 ggtgccgcac tccggacaga accgtgcctg ggcgggcacg agcgacccgc acgacgggca
     1501 ggttcgcacc aacgccgagc cgcaggaaga acagaaccgt gcggctggat cgttcgcggc
     1561 cgaacacgag gggcaggtca ccacgagatc gagtctaacg tcgagtcatt ctggggcctg
     1621 cctcgagcac ggcgacggct ccagtcgcgt cgaccgtcac ctgtatctcg tcgacgatcc
     1681 tgctcgcggt gtccggcgcc atgaccacca ccggcatctc gatgccgtac agctcggcgg
     1741 cgatcaccga gccgagagcg atcatggggt cggtctcagt catcacgatg gcggcgggcg
     1801 cggtgccgag gtggatggcc tctgcaagga cggaggaact ggaactggat cctcgacctc
     1861 gcgtcatgaa catgacgcaa ccggtcaggc atacgcccag ttgaggatgg tggatgtcgg
     1921 tcaccatgcc gctctgcggg tcgaagccac cccagaagct caggggttcc tgcatggtga
     1981 gtgcctcgcc gcgaccgatc cctcccgcga gcgtgcgccc tcgtgcctcg atcactcgcc
     2041 tgccccccac aaggcgtcat ccctgaccag ccgcccttcg acggccgagc ggatgcactc
     2101 ctggaggctg gcgatcgcga cctcgacgcc gaggttgctc ggggcgtacc acgcccattt
     2161 cgctgagtcg gtcacgacgg ggccgacccc tgcagcgatg accggcgtga tgtacgtaca
     2221 cgtgtcggtg accaccgttg cacccgcggc ctcggcgacg gcgaccgttc cggcttcctc
     2281 agcgacacgc agcacatcgc gaccggtgtt cacgtagaag ggcaccgcga accgtcggcc
     2341 gtcaaccagg gaggccagtc gctcgaagcc cgcgaccgac agatggggtg tcccgacgct
     2401 gaccgcgccg acagtggcgc cgacccccac ggtcgacaac gcatcgcgcg ccgcccgcag
     2461 gtcctcgggt ccgacgcgaa cctcgcgcgc agcggaaccg tcaccgatcg atgcctccag
     2521 cgtcggtgct tccggcgtga ccccgaccac gtggatgagg gcgaccgacc ccgacgttgc
     2581 ggcggcggcg cacatggcct tcaggtggtc ctcgctggtg gtgggtgccg gaggcagtcc
     2641 tacgacggta gggatcaggg cgccggcctc ccgtccgacc aggaggccga tggcggcgtg
     2701 cgcgaccggg tcggtgagca accgctcggg cacgccctcc acccggaaca caacgcctgc
     2761 acgacgatgc tcatccaggt gcagacccgc cgcgggtgca cgccccgtga tcgcgcagca
     2821 gatgtcgatg aaatcgctgt agcgctcggt tcgcgcgccc aacaccgagt tggcgaacac
     2881 gatcgcgttc gactcggccc acgcgatctg gtcgccgaac ccggggcgca ccggcgagtg
     2941 gtacggcgcg catgtccatg tggggcggca ccccatcgcg acgtaggcat ccatctgttc
     3001 ccgagccagc gtcgaggtcg tgttatcgag ccgcacgagt tcgggatgca gcaggtcgag
     3061 cgaggacacg ttcagcgtgc tgggcaccgc gacctgggct gccccgtcgc ggaggcgtcg
     3121 tgcgaagtcg agccctgcca tccca
//
LOCUS       DATDBZ010000465         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1166519, whole genome shotgun sequence.
ACCESSION   DATDBZ010000465 DATDBZ010000000
VERSION     DATDBZ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166519"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VIX62_10980"
     CDS             <1..584
                     /locus_tag="VIX62_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5860801.1"
                     /translation="TEPDLTLRIAALLHDAGKPATREITDDGVQFHHHEMVGARMADK
                     RMRELRYPNHMIDDVHKLVEMHLRFHGYGDGWSDAAVRRYVRDAGPLLDQLNQLTRAD
                     CTTRNEQKAARFARLQDELEERIARLAEQENLEAMRPPLNGAQVMEHLGIEPGRIVGE
                     ALEHLMELRLERGPIDEAEAYALLDAWAAQQDI"
     gene            complement(587..>2938)
                     /locus_tag="VIX62_10985"
     CDS             complement(587..>2938)
                     /locus_tag="VIX62_10985"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY5860802.1"
                     /translation="GRDPSFLLRGARLEQAETWSGTTDLGVGRPQLEYIKASTDRRDV
                     DRGLEDERHEQEIRIERRSRLRLRALVAVFAVAAIVAATLTIVATDQAKRASDAARIA
                     RARGLAGAAISDLQADPERSVLLAVAAVNETRTVDGSVLPEAEEALHRAIATSRVVMT
                     VPGAGGEVAWGSAGTFAATSSDDPGTIVVADEETGETVRRLDAHVGLVTGLAFHPDGS
                     LLASTGDDGTLRLWDNSTGEALKTVRGTGVASQVSFTADGSVAAAMWPDHGLIRFVDT
                     ETQRVVDTLRGTGSIVMALSPDGGAVAVDGNHGLSVIEMETHRQRFAPRPVPFGAGVI
                     AWSPDGRYLATGGIPGAMIWDAETGDLLDELPGHPWIEEISWSADAATLVTAGDDAPG
                     AKVWDIQEGIADERLALTSIETERGIGGIALSPDDARVMAGATDLTAVKVWDLSGAGN
                     AEWASFPTVGEFGDVGFLPDGRLVAADIDGGIRVHDLESGVRSPRIGPPVNEHMFSVS
                     PDGTVVAIAYGKKSRYPGEVHLWRTDTGDELPVTRTIEGDLDGVDWSPDGTRLVIADP
                     TRHATQIVDRSGVVVVELPDQAAFSTWAARFSPDGRLVATIGGDEERGSLISIWDWRR
                     GELLRTMHTDFAVGLTFDPSGERILTMFGPPVIWDVETGARIGTLDEYPNGVGTVAFS
                     HDGSRLAATASGNVVLFDPETGTKQFVLRTPDLLQPLRLAFNEDGSMLATQAPNPLGD
                     EPGIVRVWALDIDDALDLARREVTIALSDQECRRYWYVDDCST"
BASE COUNT          470 a         1040 c          970 g          458 t
ORIGIN      
        1 ggaccgaacc cgacctgacg ctgcgcatcg cggcgttgct ccacgatgcc gggaagcccg
       61 ccactcgcga gatcaccgac gacggcgtgc agttccacca tcacgagatg gtgggcgccc
      121 gcatggcgga caagcgcatg cgcgaactcc gctatcccaa ccacatgatc gacgacgtcc
      181 acaagctggt ggagatgcac ctgcggttcc acgggtatgg cgacggatgg agcgatgccg
      241 cggtgcgccg ctacgtccgc gatgccggcc cgctgctcga ccagctcaac cagctcaccc
      301 gcgccgactg caccacgcgc aacgagcaga aggcggcgcg gttcgcacgg ctgcaggacg
      361 agttggaaga gcgcatcgcg cgactcgccg agcaggagaa cctggaggcc atgcgcccgc
      421 cgctgaacgg cgcccaggtg atggagcacc tcggtatcga acccgggcgc atcgtcggcg
      481 aggcgctgga gcacctgatg gagcttcgac tcgagcgcgg tcccatcgac gaggccgaag
      541 cgtacgcgtt gctcgacgcc tgggccgcac agcaggacat ctagcgtcac gtcgaacagt
      601 cgtcgacgta ccagtaccgc cggcattctt gatcggagag cgcgatcgtg acctcgcgcc
      661 tggccaggtc gagcgcgtcg tcgatgtcga gcgcccacac gcgcacgatg ccgggttcgt
      721 cgcccagcgg gttgggggcc tgggtcgcga gcatggaacc gtcctcattg aacgcgaggc
      781 gcaacggctg gagcaggtcg ggtgtgcgga ggacgaactg cttcgttccc gtctcggggt
      841 cgaacaggac cacgttgccg gaagcggtgg cagcgagccg gctgccatcg tggctgaacg
      901 ccaccgtccc caccccgttg gggtactcgt cgagcgtgcc gatccgggcc cccgtctcca
      961 cgtcccagat caccggtggg ccgaacatcg tcaggatccg ttcgccggac ggatcgaagg
     1021 tgagcccgac agcgaagtcg gtgtgcatcg ttcgtaggag ctccccccgg cgccagtccc
     1081 agatcgagat caagctgcct cgctcctcat caccgccgat ggtcgcaacg aggcggccgt
     1141 cggggctgaa tcgagctgcc catgtgctga acgccgcctg atcggggagc tcgacgacca
     1201 ccaccccgga ccggtcgacg atctgggtcg catgacgcgt ggggtcggcg atcacgagtc
     1261 gggtcccatc cggactccaa tccaccccgt cgagatcccc ttcgatcgtt cgtgtgacgg
     1321 ggagttcgtc gccggtgtct gtgcgccaaa ggtggacttc gcccgggtag cggctcttct
     1381 tcccataggc gatcgcgacg acggttccgt cggggctgac ggagaacatg tgctcgttca
     1441 cgggcggacc gatccgaggc gaccgcaccc cgctctcgag gtcgtggacc cggatcccac
     1501 catcgatgtc ggccgctacc aaacgcccgt cgggcaggaa ccccacgtcc ccgaattcac
     1561 ccacggtggg gaagctcgcc cattccgcgt tccccgcgcc actgaggtcc cagaccttca
     1621 cggcagtgag gtcggtggct ccagccatca cacgtgcgtc gtcgggggag agcgcgatcc
     1681 caccgatccc acgctccgtc tcgatcgagg tcagggccag tcgctcgtcc gcgatcccct
     1741 cttggatgtc ccagaccttc gcacccggtg cgtcgtcgcc ggccgtgacc agggtggcag
     1801 cgtcggcgct ccacgaaatc tcctcgatcc acggatgccc cggaagttcg tcgaggagat
     1861 cgccggtctc cgcgtcccag atcatcgcac cagggatacc cccggtcgcg aggtaccggc
     1921 cgtccgggct ccaggcgatc actcccgcgc cgaaaggtac cggtctcggc gcgaagcgct
     1981 gtcgatgcgt ctccatctcg atcaccgaga ggccgtggtt gccgtcgaca gcgaccgctc
     2041 caccgtcggg actgagcgcc atcacgatgc tcccggtgcc ccgaagggtg tcgacgacgc
     2101 gctgggtctc ggtgtccacg aaccggatca ggccgtggtc tggccacatc gcggcggcga
     2161 cggagccgtc ggcggtgaag ctcacctggg acgcgacccc cgtgccgcgg accgtcttca
     2221 gcgcctcgcc cgtcgagttg tcccacaggc gcaaggtgcc gtcatcgccg gtggaggcca
     2281 gtagcgagcc gtcgggatgg aaggcgagtc cggtgacgag accgacgtgt gcgtccagcc
     2341 tccgcacggt ctcgccggtc tcctcgtccg cgaccacgat cgttccggga tcgtcggacg
     2401 aggtcgcggc gaaggtgccg gccgaacccc acgccacctc accgcccgcg ccgggcacgg
     2461 tcatcaccac ccgcgacgtc gcgatcgctc gatgcagtgc ctcctccgcc tcgggcagga
     2521 ccgacccgtc gaccgttcgg gtctcgttca ccgccgccac cgcgagcagc acgctgcgtt
     2581 cggggtccgc ttgcagatcc gagatcgcgg cgccggccaa tccccgtgcc ctggcgatcc
     2641 gcgcggcatc cgacgcacgt ttagcttggt ccgtcgcgac gatcgtgagg gttgcggcca
     2701 cgatggcggc gaccgcgaag acggcaacta gggcgcgcag gcgtaaccga gagcggcgct
     2761 cgatgcggat ctcctgctcg tgccgttcgt cttcgagccc acgatcgaca tctcgtcgat
     2821 cggtgctggc cttgatgtac tcgagctgag gcctgccgac gccgagatcg gtggttcctg
     2881 accacgtctc cgcctgctcg aggcgggctc ctcgcaacag gaagctcgga tcccggcc
//
LOCUS       DATDBZ010000466         2856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_358413, whole genome shotgun sequence.
ACCESSION   DATDBZ010000466 DATDBZ010000000
VERSION     DATDBZ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358413"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VIX62_10990"
     CDS             <1..321
                     /locus_tag="VIX62_10990"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY5860803.1"
                     /translation="QRSFVDTLDFRTSPGHSGDPAHDAARGWHGSGPTAVVTDLGTYG
                     FDEVTGEMTLRTMHPGVALDDVRANMGWEPRIAADLGETPPPSVEELRLIREELDPAG
                     IYTK"
     gene            569..1870
                     /locus_tag="VIX62_10995"
     CDS             569..1870
                     /locus_tag="VIX62_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5860804.1"
                     /translation="MFPRVFDRDLSTAVDAEGAWIIDGDGRRFLDGAGGAIVVNVGHG
                     RRAVIDAITAQLHRTQYVHGTAFTTEAVETYADEVAAVLPMDDPRIYPVSGGSEAVET
                     ALKLARTYHLANGEASRFTVIARRSAYHGNTLGALDASGKEPLRKPYTPWLGRYLHAP
                     AAYEYRCENPQHPDACGAWHAGELERMIHQAPPKSVAAFIAEPIAGATLGAAVPCEDY
                     WPAVTAICRDHGVLVIADEVMTGFGRSGRWFGADHWAVRPDILTAGKGSTSGYVPFGF
                     VAATDRVYEAVATTGFVHGFTWSHNALGAAAGLAVLQILRDEDLITRSAALGARLRAS
                     LHAALEDCSIVGDVRGLGMMIGVELVRDPDTKEPFPRTQRVTERVLAAARDAELLLYS
                     STGHVDGTNGDLVMLGPPFVLTGEDEAILVERTVAAIRSVA"
     gene            1867..>2856
                     /locus_tag="VIX62_11000"
     CDS             1867..>2856
                     /locus_tag="VIX62_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEY5860805.1"
                     /translation="MSGTAGSVYVPQARIYDHGPGHPLRPERVLLTWDLIQAYGLDAD
                     PHVEILGAEPADISTLLLVHTPEYIDATQRAGRGDDGSWARFGYGPGDNPIFDQMHEA
                     GAIVAGATVGAAKAVLSGQVAHAFNAAGGLHHAMASRASGFCVYDDPAIAIAWMLDQG
                     VERVAYVDVDVHHGDGPQAIFYDDPRVLTISIHEYAPHLGFFPGTGGPDETGGPSAPG
                     SAINVPLDPGTGDDVWLEAFREVVPPAVTAFAPDVLVTQLGCDTHASDPLAHLRLTTN
                     TYRTTARILHALAHDAAGGRWVATGGGGYQWARVVPRAWTTYFAEMADVELPDA"
BASE COUNT          465 a          944 c          985 g          462 t
ORIGIN      
        1 cagcgttcgt tcgtggacac gctggatttc cgcaccagcc ccggacacag cggtgatccc
       61 gcgcacgacg ctgcgcgcgg ctggcacggc agtggcccga cggcggtcgt caccgacctg
      121 gggacgtacg ggttcgacga ggtgacgggg gagatgaccc tgcgaacgat gcacccgggg
      181 gttgcgctgg acgatgtgcg tgccaacatg gggtgggaac cgaggatcgc agccgacctg
      241 ggcgaaaccc cgccgccctc ggtggaggag ctacgcttga tccgcgagga gttggacccc
      301 gcgggcatct acacgaagtg atcatgtcgg atcgagacga ccgcttgtgg cgtgatgggg
      361 acgaagagcc cgagcccgat tgggccgagc agatccgcgc tgctcgcaag gctcgcgggg
      421 acaggcttcg aggagtgttc gccacgttcg aggacgacga cccggcctcg ccgctccctg
      481 tcaagcagcc gccctcatcc agatctgcgc ccccgcctgc tgacgtcgag ttgtacgacc
      541 acgagcaaga gtcgccacct tgagccacgt gttcccgcgg gtgttcgatc gagatctgtc
      601 gacggccgtc gatgccgagg gcgcctggat catcgacggg gatggccggc gcttcctgga
      661 tggtgcaggc ggcgcgatcg tcgtcaacgt cggtcatggc cgacgcgcag tgatcgacgc
      721 catcaccgcg cagctgcatc gcacgcagta cgtccatggc acggcgttca ccacggaggc
      781 ggtggagacc tacgcggatg aggtcgccgc ggtcctgccc atggacgacc cccgcatcta
      841 ccccgtctcg ggcggcagcg aagcggtcga gaccgccctg aagctggcgc gcacctacca
      901 cctggccaac ggtgaggcct cacggttcac cgtgatcgca cggcgcagtg cctatcacgg
      961 gaatacgctg ggcgcgctcg atgccagcgg caaggaaccg ctgcgcaagc catacacgcc
     1021 gtggctgggg cgctacctgc acgcacccgc cgcttacgaa taccgatgcg agaatcccca
     1081 gcaccccgat gcctgcggcg cctggcacgc cggcgaactc gagcgcatga tccatcaagc
     1141 accgccgaag tcggtggcag cgttcatcgc cgagccgatc gcgggcgcca ccttgggcgc
     1201 cgccgttccg tgtgaggact attggcccgc ggtcacggcg atctgccgcg accacggagt
     1261 gttggtgatc gccgacgagg tgatgacagg gttcggtcgc tcgggacgct ggttcggcgc
     1321 cgaccactgg gccgtgcgtc ccgacatctt gaccgccggc aagggctcca cgagcggcta
     1381 cgtgccgttc ggtttcgtgg cggcgaccga ccgtgtctat gaagcggtcg cgaccacggg
     1441 gttcgtgcac gggttcacct ggtcgcacaa cgcgctcggt gccgccgcag gcctcgccgt
     1501 gctgcagata ttgcgtgacg aagacctgat cactcgcagt gcggcgctgg gtgcacggtt
     1561 gcgcgcgtcg ctgcacgctg cgctcgagga ctgctcgatc gtgggggacg tgcgaggact
     1621 cgggatgatg atcggcgtcg aattggtccg tgaccccgat acgaaggaac ctttcccgcg
     1681 cacccaacgt gtcaccgaac gggtgctggc cgcggctcgc gacgccgagc tgctgctgta
     1741 ctcctcgacg gggcacgtgg acggcacgaa cggtgacctg gtgatgttgg gaccgccctt
     1801 cgtcctcacc ggcgaggacg aagcgatcct cgtcgagcgc acggtggctg cgatacgttc
     1861 ggtcgcgtga gcggaacggc gggatcggtc tacgtaccgc aggcccggat ctacgatcac
     1921 ggtcccggtc acccattgcg tccggaacgg gtgcttctca cgtgggacct gatccaggcc
     1981 tacggattgg atgctgatcc tcacgtcgag atcttgggcg ccgaacccgc cgacatctcg
     2041 acgctcctgt tggtgcacac ccccgagtac atcgatgcca cgcagcgagc cggtcgtggc
     2101 gacgacggtt cgtgggctcg gttcggctac ggtcccggcg acaacccgat cttcgatcag
     2161 atgcacgaag caggagcgat cgtggccggc gcgacggtgg gcgcagcgaa ggcggtgctc
     2221 tccggtcagg tggcgcacgc attcaacgcc gcgggcggac tccaccacgc gatggcttcg
     2281 cgagcgtcgg ggttctgcgt gtacgacgac cccgcgatcg cgatcgcatg gatgctcgac
     2341 cagggcgtgg aacgggttgc ctacgtcgat gtcgacgtgc accacggcga cggaccacag
     2401 gcgatcttct atgacgaccc ccgggtcctg acgatctcga tccatgagta cgcgccgcac
     2461 ttgggtttct tcccgggcac aggaggcccg gatgaaaccg gtggcccatc ggcgcccggt
     2521 tcggcgatca atgtgccgct cgatccgggt accggcgacg acgtgtggct ggaggcgttc
     2581 cgcgaggtcg tcccgcccgc tgtgacagcg ttcgctcccg atgtgctcgt gacccaattg
     2641 ggctgcgata cgcacgcgag cgatccgctg gcccacctgc gcctgaccac caacacctat
     2701 cgaacgaccg ctcggatcct gcatgcgctt gcgcacgacg ccgccggcgg ccgatgggtt
     2761 gcgacggggg gcgggggcta ccaatgggca cgcgtcgtgc cacgagcatg gacgacgtac
     2821 ttcgcggaga tggcagacgt cgaactgccc gacgcg
//
LOCUS       DATDBZ010000467         4042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1377339, whole genome shotgun sequence.
ACCESSION   DATDBZ010000467 DATDBZ010000000
VERSION     DATDBZ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377339"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VIX62_11005"
     CDS             <1..842
                     /locus_tag="VIX62_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5860806.1"
                     /translation="HEEMLRHYEPGGLLPRIVAGLQALGKDLDSVTHEDLAPADEFHS
                     AGRSATRALVELAKIPPGSRVLDVGSGLGGPARYLAATLGCDVTGIDLSPEFCGVANA
                     LSRMTRLSDRTRFQAGDALELPFAASSFDVVWTIQMQMNIRDKRRLYSGIARVLRPGG
                     LFVCQEICAGNGEPIELPVPWASRPNQSHLADAESLRGLIRGAGLLERTWRDITADIV
                     AARKAQQAKAQASGASGSSAPPPLGMHLVLGEQAAVKMSNSGRNTDAGRTVFIQGVFD
                     KPG"
     gene            964..1361
                     /locus_tag="VIX62_11010"
                     /pseudo
     CDS             964..1361
                     /locus_tag="VIX62_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145875.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain containing protein"
     gene            1882..3555
                     /locus_tag="VIX62_11015"
     CDS             1882..3555
                     /locus_tag="VIX62_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860807.1"
                     /translation="MHREMRGRDGVGGGQRRTRAVQRVRPSVSPASPVPGVQGLFFDL
                     QESAGNRAVSRMLGVRPVVQRTQLYVFGGGRVTDFNGPGTNERKSVELVQKRLVALGV
                     LLAADAAADTARITARTGRIMAGDISNTTAAIDRCDAQSVSEPMARTLLKANLVAGVG
                     TGETNDAADVDLVLNLLHEEHHVTNDEFDAGEVVIEGVGRTVDPALVPGFLTGLTRLK
                     RGYAGGYPFRGTTKRRKKLLVTQGTAAYQRALDYNNAGRAAMQTWLESAAGQSRDKVL
                     RNSAEWCLSGRIKLYCTTKTHDSPARVKANGSPKRFYAIFGHPLGALSERQVPYLRKL
                     KGETTFDNANVAIEAPSGGSAPAGAITVVDPVPSGRRAFYETIKHEVQHTADHHPATD
                     EGRYKTELNARWIDGAYTMYSPRRRVRAKGYTWNERQYAVFLNLWKYKDLYPYVRENW
                     NDPDAGKRAAWRTMIVGYKKPASFNPINSVRVESLHEAIAACTPADCDADDLFRAGKG
                     PENAKAAAVKRAMAALDSLDRATIKTNTDLKASAAVNLTGKLRDEYSAL"
     gene            3635..>4042
                     /locus_tag="VIX62_11020"
     CDS             3635..>4042
                     /locus_tag="VIX62_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860808.1"
                     /translation="MSDRSVGWSAPDGSRLDVTPDHAWECADAGGGAIVIAGEGWLTW
                     RGGRDAVRVEAPEVHRVAVAGGLVAGATADGHVLVWPDLEDSPAAQRAQVRFEPDDLV
                     CDAAGGRLIVSGWLDDADAVMTVLNVRVDEPVEV"
BASE COUNT          710 a         1323 c         1396 g          613 t
ORIGIN      
        1 cgcacgagga aatgcttcgt cactacgagc cgggcggact tctgccgcga atcgtggcgg
       61 ggttgcaagc tctcggcaag gacctggact cggtcacgca cgaagatctc gcgccggccg
      121 acgagtttca ctccgcggga cgttcggcca cgcgcgcatt ggtggagctt gcaaagattc
      181 cgccaggatc gcgagtgctc gacgtgggca gcgggttggg cggcccggcg cgttacctcg
      241 ccgcaacttt gggttgcgac gtgacaggca tcgatctctc gcctgaattc tgcggggtgg
      301 ccaacgccct ttctcggatg acgcggttgt ccgatcgcac ccgctttcag gcgggtgatg
      361 ccctggagct tccgttcgcc gcgtccagct tcgacgtggt gtggacgata caaatgcaga
      421 tgaacatccg ggacaagcgc cgcctgtatt ccggaatcgc ccgggtactc aggccgggcg
      481 ggctgttcgt ctgccaggaa atctgcgcgg ggaacggcga gccgatcgag cttccggtcc
      541 catgggcgag ccgccccaac cagagccatc tggccgatgc ggaatccctc cgcgggttga
      601 tccgcggtgc aggtctcctg gagcgcacct ggcgcgacat taccgccgac atcgtggcgg
      661 cgcgcaaggc acagcaggcg aaggcgcaag cgtcgggtgc gagcggctcc agcgcccctc
      721 ctccgcttgg aatgcatctc gtgctgggag aacaggcagc cgtcaagatg agtaactccg
      781 ggcgcaacac cgacgccggg cgcacggtgt tcatccaggg cgtattcgac aagcctggct
      841 agtggtccga tccgctgatc ccgctggaaa actctgtcga aagcacgggc gcggcgaatc
      901 tccattgatc gtccggtcaa agggcacatt cggtcacgac gaccgaaatt cgcccgctga
      961 gggatcggtg gattctggcg gtcatcgcaa caggatgtct ccgatgactt cgatcggtgg
     1021 gacgcctgga attaccgtga ttcgtcggct cgcgcagctg agtccggcac catcgtgggc
     1081 tatcgcgttc acgcctccga tggcgacatc ggcaaggtcg acgaggccag caacgcggtc
     1141 gatgaaagca gcatcgtggt cgacaccggc ccatggatct tcggccggaa ggtaattctt
     1201 ccggcgggca cgattcagcg tgtcgacgac gcggacgaga ggtctacgtc gatctcacga
     1261 aggcccagat caaggattcg ccagaactcg atgatgccac cgctgacgat cccgcctatc
     1321 gagaccggct cgggacctat tacggcgagt tctaccgtta gtcccgcggg tgcccggcgc
     1381 ctgccgggca cccggcgccg ctgcccgctt cgccaaccca tccacccatc cacgggatca
     1441 gcacactcaa actcagggga gtccgcactg gacgtcctcg cgtcggcctg ccatgtagaa
     1501 gccgactgcg atcggtgaag gaaccttcta cgggacggtt gccaacgatc tcgtgaccag
     1561 tgcccgggta gggatccata ccgggcggcg gctgccgcgt gccagtcgcg gagtttctca
     1621 gagagctcat cgccgccgac gaggcgcgtc gcgccgagcg atcggaagcc agggcgtgag
     1681 cgtctcggtc accgcccgcc ccatcggcgg gccgccgagc atctgcgcga gcttcccgat
     1741 gccgtggagc gactgcgggt gctgtcccgc gactgagtcc ggattcgcgc cgcggcagca
     1801 gtggggaaag cgtgccggct gcccgcggag gtgcccgaaa ggaccttgca ggaacgcagt
     1861 caccgggtca gagttcggag tatgcatcgc gagatgcgtg gccgcgatgg tgtcggcggc
     1921 ggacaacggc ggacccgcgc cgtgcaacgt gtccgtccgt cggtgtcgcc cgcgagtccc
     1981 gtgcccggcg tgcagggatt gttcttcgat ctgcaggagt ccgcgggcaa tcgcgccgtc
     2041 agccggatgt tgggcgtgcg acccgtcgtg cagcgcacgc agctctacgt gttcggtggc
     2101 ggccgcgtca ccgacttcaa cgggccgggc acgaacgagc gcaagtccgt ggagctcgtg
     2161 cagaagcggc tcgtcgcact gggtgtgctc ttagcggccg acgccgctgc ggacaccgcg
     2221 cgaatcacgg cacgaacggg ccggatcatg gcgggcgaca tctcgaacac cacggccgcc
     2281 atcgaccgct gcgatgcgca gtcggtgtcg gaaccgatgg ccaggaccct gctcaaggcg
     2341 aacctggtcg ccggcgtcgg cacaggggag accaacgacg cggccgacgt cgacctggtg
     2401 ctcaacctgc tgcacgagga gcatcacgtc acgaacgacg agttcgacgc cggcgaggtc
     2461 gtcatcgaag gcgtcggtcg cacggtcgac cctgcgctcg tgccggggtt cctgaccggt
     2521 ctcacgaggt tgaagcgcgg gtacgcgggc gggtacccgt tccggggcac gacgaaacgg
     2581 cggaagaagc tcctcgtgac ccaggggacc gcggcatacc agcgagccct cgactacaac
     2641 aacgccgggc gcgcggcgat gcagacctgg ctcgaatcag cggccggcca gtcgagggac
     2701 aaggtgctgc gcaactccgc cgagtggtgc ctctcgggca ggatcaagct ctactgcacg
     2761 acgaagaccc atgactcgcc cgcgcgggtg aaggcgaacg gctcacccaa gcggttctac
     2821 gcgatcttcg gtcacccgct cggcgccctg agcgagcgcc aggtgccgta cctgcgcaag
     2881 ctcaagggcg agaccacgtt cgacaacgcc aacgtggcga tcgaggcccc ctcggggggc
     2941 tcggcgcccg ctggtgcgat caccgtggtc gacccggtgc cgagcggcag gagagcgttc
     3001 tacgagacga tcaagcacga ggtgcagcac accgctgacc accacccggc gaccgatgag
     3061 gggcgctaca agaccgagct caacgcccga tggatcgatg gggcctacac catgtactcc
     3121 ccccgacgcc gggtgcgagc gaaggggtac acgtggaacg agcggcagta cgcggtcttc
     3181 ctgaatctct ggaagtacaa ggacctgtat ccgtacgtgc gcgagaactg gaacgatccc
     3241 gacgcgggca agcgcgccgc ctggcgcaca atgatcgtcg gctacaagaa gcccgcctcg
     3301 ttcaatccca tcaactcggt gcgagtcgag agcctgcacg aggcgatcgc ggcatgcacg
     3361 cccgcggact gcgatgccga cgacctgttc cgggcgggca aagggcccga gaatgcgaag
     3421 gccgcagccg tgaagcgggc gatggccgca ctcgattccc tcgaccgtgc gacgatcaag
     3481 accaacaccg acctgaaggc gagcgccgca gtcaacctca cgggcaagct ccgcgacgag
     3541 tactcggcgc tgtgagcaga gacgagcgga gatgaccagg aacccatccc gcgctcgagg
     3601 gatcatcgac cccgacaccg ggagcctcct cgagatgagc gaccggtcgg tcggctggtc
     3661 ggcgcccgac ggttcgcgcc tggacgtcac cccggatcac gcgtgggagt gcgcggatgc
     3721 aggcggtggc gccatcgtca tcgccggcga aggatggctc acctggcgcg gcggtcgcga
     3781 cgccgtccgg gtcgaggcgc cggaggtgca ccgggtcgcc gtcgcgggcg gtctcgtggc
     3841 cggagcgacc gccgacggcc atgtgctggt atggccggat ctcgaggact cgccggccgc
     3901 gcagcgcgcg caggtccggt tcgagcccga cgacctcgtg tgcgatgccg ccggggggcg
     3961 gctcatcgtg tcgggctggc tcgacgacgc cgacgccgtc atgacggtgc tcaacgtgcg
     4021 ggttgacgag ccggtcgagg tg
//
LOCUS       DATDBZ010000468         3776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_137250, whole genome shotgun sequence.
ACCESSION   DATDBZ010000468 DATDBZ010000000
VERSION     DATDBZ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137250"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VIX62_11025"
     CDS             <1..1176
                     /locus_tag="VIX62_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5860809.1"
                     /translation="FLAYINVATPPDAKSPSIADAFMGLKLGKAFRDLGAKTGREAIR
                     ALPMAAADLVQEVFEEEAIRGPLATRGVLFTSMGPWATGTAAVFLNDSAGNDGGAAGT
                     AVFARGGTGALASALEQAAKSLGVDIRTDTRVAAIRSKDGRVLGVTLADGTEIDSPLV
                     VSGADPKRTLRLCDPVELNPYEVWRTENIRQPGATATINLALSGLPTFTGAADASALQ
                     GRIVVGLSIDHVEKAMDAYKYGHVAEDPILEATIPSLVDPSLAPEGKHVMSILFQAAP
                     RTLRDASWSDERGRLADLAVNSLERFAPGLGGLVEASQVISPEDMENDYGLTGGHVYH
                     AEPALDQFFAWRPLNGEARYQLQIKGLYLAGSGAHPGGGVTGGPGANAARQILSDTK"
     gene            1238..2338
                     /locus_tag="VIX62_11030"
     CDS             1238..2338
                     /locus_tag="VIX62_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5860810.1"
                     /translation="MAMISLQQIEKRYPGTSAPAVGALSLDIEEGETVALVGPSGCGK
                     TTTLKMINRLIEPTGGVLLLQGTNVLDRDPVDLRRGIGYVIQHTGLMPHRTIRQNMTT
                     VPSLLGWDAATAAQRVEELTEIFDLDPELLDRYPAELSGGQRQRVGVARALAADPPVM
                     LMDEPFAAVDPIVRARLQDQFLDIQERLKKTIVFVTHDIDEAIKLSDRIAILNVGGVL
                     EQFAPAEEILREPANDFVREFVGAERGLKRLALIKVGDIEVEPGPVVASNASADEARA
                     AIEAGGYDFVSVVDDGELLGWIDRDMVRDEEGISGIAPRPFSAYVTARDSLRQALDSI
                     VTSRTGVAVVVTEGQRYRGVLSLERISQEIIA"
     gene            2335..3054
                     /locus_tag="VIX62_11035"
     CDS             2335..3054
                     /locus_tag="VIX62_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860811.1"
                     /translation="MIGPTFHQAAAVVAQIDPDEPLVRWIWISDNVDEIVSRGLQHLY
                     LTLVAVAVGFVIAFPMAILASRYRWVVRPATWISGVLYTIPAIAFIFLLLPLTGLSLT
                     TVAVPLIAYSLLILFRNTLAGLDAVDRDVKEAAVGMGFSSRQLLWRVEIPLALPVIIA
                     GLRIATVSAIGLVTVAALLGRGGFGQFILEGVDRFFPTPLVVGVVLSVALAIVADLLL
                     LGLQRLITPWAKTAGTRTVAT"
     gene            3062..3775
                     /locus_tag="VIX62_11040"
     CDS             3062..3775
                     /locus_tag="VIX62_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860812.1"
                     /translation="MNGFVEGFQWLFEPMHWSGTDGIPMRVWEHVQLSFIALVIASLI
                     AIPLGLLVGHTRRGAFLTVQLANVGRAVPSIAVLGVMYLVVLQLSPKLAFGFAPTIVA
                     LVLLSIPVILINTLVGVQQVDPDTVEAARGMGMNGRQVLFRVEVPMATPLILTGLRLA
                     AVLVVATAGLAALIAGGGLGRHIVDGVALRETDRVVAGSILIAILAILTDVLFTYLAN
                     VLSPRLTSDGRGLPSPEQP"
BASE COUNT          570 a         1238 c         1292 g          676 t
ORIGIN      
        1 ttcctggcct acatcaatgt ggcgacgccg cccgacgcga agtcgccgtc gatcgccgac
       61 gcgttcatgg gcttgaagct gggcaaggcg ttccgcgacc tgggtgccaa gaccggccgc
      121 gaggcgatcc gcgcgttgcc gatggcggcc gccgacctgg tgcaggaggt cttcgaggag
      181 gaagccatcc gcgggccgct cgcgacgcgc ggtgtgctct tcacgtcgat gggcccttgg
      241 gccacaggca cggcggcggt gttcctgaac gactccgccg gcaacgacgg cggcgcagcg
      301 ggcacggcgg tgttcgcacg cggcgggacc ggcgcattgg cgtcggcgct ggagcaagcc
      361 gcgaagagcc tcggtgtcga catccgcacc gatactcggg ttgcagcgat ccggtcgaag
      421 gacggtcggg tgctgggcgt gacgcttgcc gacggtaccg agatcgattc accgctcgtg
      481 gtgtcgggcg ccgaccccaa gcgcaccctg cgtctgtgcg acccggttga gctgaacccc
      541 tacgaggtct ggcgcacgga gaacatccgt cagcccggcg ctaccgccac gatcaacctg
      601 gcgctctcgg ggttgcccac gttcacaggg gcggccgatg cttcggcgct gcagggtcgg
      661 atcgtggtcg ggctctcgat cgaccacgtc gagaaggcga tggacgcata caagtacggg
      721 cacgtcgccg aggacccgat cctagaggcg acgatcccgt ccttggtcga cccctcgctc
      781 gcgcccgagg gcaagcacgt gatgtcgatc ctgttccagg cggcgcctcg cacgctgcgt
      841 gacgcttcct ggtcggacga acgcggccgc ctcgccgacc tcgcggtgaa ctccttggag
      901 cggttcgcgc cgggcctggg cggcctggtc gaagcgagcc aggtcatctc gcccgaggac
      961 atggagaacg attacgggct caccggcggg cacgtgtatc acgccgaacc cgccctcgat
     1021 cagttcttcg cgtggcgtcc gttgaacggc gaagctcgct accaactgca gatcaagggt
     1081 ctgtacctgg ctggctcggg cgcgcacccg ggcgggggcg tcaccggcgg tcccggtgcc
     1141 aacgccgctc gccagatcct gtccgacacg aagtagcgcg agccagaggg ttcgagcgca
     1201 ggtaggcgaa cgcgcgacgc ccggataggg tcgggcgatg gcgatgatct cgctccaaca
     1261 gatcgagaag cgctaccccg gcacgtccgc tcccgccgtc ggcgccctca gcctcgatat
     1321 cgaagagggc gaaactgtcg cgctcgtggg tccttccggc tgcggcaaga ccaccaccct
     1381 caagatgatc aaccgcctga tcgaacctac gggcggggta ctcctgttgc agggcaccaa
     1441 cgtgctggat cgcgaccccg tcgacctccg ccgtgggatc ggttacgtga tccagcacac
     1501 aggactcatg ccgcaccgca cgatccgcca gaacatgacg acggtcccat cgttgttggg
     1561 gtgggacgcc gccacagcgg cccaacgcgt cgaggaactc acggagatct tcgatctgga
     1621 ccccgaactg ctcgaccggt atccggctga gctttccggg gggcagcgtc aacgagtcgg
     1681 ggtggcgcgc gccctggcag ccgacccgcc cgtcatgctc atggacgagc cgttcgcggc
     1741 ggtcgacccg atcgtgcgcg cacgtttgca agaccagttc ctcgacatcc aggagcgcct
     1801 gaagaagacg atcgtgttcg tgactcacga catcgacgag gcgatcaaac tctccgatcg
     1861 catcgcgatc ttgaacgtcg gcggggtgct ggagcagttc gcgcccgccg aggagatcct
     1921 gcgcgaaccc gccaacgact tcgttcgcga gttcgtaggg gccgaacggg ggctgaagcg
     1981 gctcgccctg atcaaggtgg gcgacatcga ggtcgaaccc gggccggtcg tggcatcgaa
     2041 cgcttctgcc gacgaggcac gcgctgcgat cgaggccgga ggctacgact tcgtcagcgt
     2101 cgtcgacgac ggtgagctcc tcggctggat cgatcgagac atggtgcggg acgaggaggg
     2161 catctcgggg atcgctccgc gtcccttcag cgcgtatgtg acggcgaggg actcgctccg
     2221 ccaggcgctg gactcgatcg tgacttcacg caccggtgtc gccgtcgtgg tgacggaggg
     2281 ccagcgctac cgcggggtcc tatcgctcga acggatctcg caggagatca tcgcgtgatc
     2341 ggcccaacgt tccatcaagc ggcggcggtc gtcgcacaga tcgacccgga tgaaccactc
     2401 gtccgctgga tctggatcag cgacaacgtg gatgagatcg tcagccgcgg cctccagcac
     2461 ctctacctca ccctcgtcgc ggtggcagtg ggtttcgtga tcgcgttccc catggcgatc
     2521 ctcgcgtccc ggtaccgatg ggtcgtcaga cccgccacct ggatctcggg cgtgctgtac
     2581 acgatccccg cgatcgcgtt catcttcttg ttgttgcccc tgacgggcct gtcgttgacc
     2641 acggtcgcgg tgccactgat cgcctacagc ctcctgatct tgttccgcaa cacgttggcg
     2701 ggcctggacg ccgtggatcg tgacgtcaag gaggctgccg tcgggatggg gttctcctcg
     2761 cgccaactct tgtggcgggt cgagatcccg ctcgcactgc ccgtcatcat cgccggactg
     2821 cggatcgcga ccgtctccgc gatcggcctc gtgaccgtgg ctgccttgct gggtcgggga
     2881 ggattcggcc agttcatcct ggagggggtc gaccggttct tccccacgcc gctggtggtt
     2941 ggcgtggtgt tgtcggtggc gctcgcgatc gtcgccgatc tgctcttgct gggactccag
     3001 cgtctgatca ccccgtgggc gaagacggcc ggcacccgaa cggtggccac gtgaggcgac
     3061 cgtgaacgga ttcgtcgagg gtttccagtg gctgttcgag ccgatgcatt ggtcggggac
     3121 cgacggcatc cccatgcgcg tgtgggagca cgtccagctc tcgttcatcg ccctggtgat
     3181 cgcgagcctg atcgcgatcc cgctgggcct gctggtgggg cacacacgcc gcggggcgtt
     3241 cttgacggtg caactcgcga acgtcggtcg tgccgtgccc tcgatcgccg tgctgggcgt
     3301 catgtatctg gtcgtgctgc aactctcgcc caagctcgcg ttcgggttcg ctcccacgat
     3361 cgttgcactc gtcctgctgt cgatcccggt gatcttgatc aacacgctcg tgggtgtgca
     3421 gcaggtcgac ccggacacgg tcgaggccgc ccgtgggatg ggcatgaacg gcaggcaggt
     3481 gctcttccgg gtggaggtgc cgatggcgac ccctttgatc ttgaccggct tgcgtctggc
     3541 ggcggtcttg gtcgtggcga ccgccggtct ggcagctctg atcgcgggcg gcggattggg
     3601 ccggcacatc gtggacgggg tcgcccttcg ggagaccgac cgggtggtcg cggggtccat
     3661 cctgatcgcg atcttggcga tcctgacgga cgtgctgttc acgtacctgg cgaacgtgct
     3721 ctcgcctcgg ctcacatctg atggtcgagg gctgcccagc cctgagcagc cttaag
//
LOCUS       DATDBZ010000469         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2818610, whole genome shotgun sequence.
ACCESSION   DATDBZ010000469 DATDBZ010000000
VERSION     DATDBZ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2818610"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VIX62_11045"
     CDS             <1..1354
                     /locus_tag="VIX62_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY5860813.1"
                     /translation="DSVEYQYMVERRRAQSGSLPLRTTRVRRPLGLPSNDAYAEVLLG
                     SGKQAASTTTAFTRLLRNLCRDDTVGSRVVPIIPDEARTFGMDALFKELKIYASQGQK
                     YQPIDASMLLSYTEASDGQILEEGITEAGAMASFIAAGTSYATHGVPMIPFYTFYSMF
                     GFQRVGDLIWQAADARARGFLMGATAGRTTLLGEGLQHQDGHSHVHASTVPACQAFDP
                     AFAYETAVIVKDGIRRMFGPNPEDIFYYLTVYNENYPMPAMAERDGIEAEIVRGLYHW
                     AHAPEGVSKKATVLFSGSAQGAAREAAAQLAEHYDVGVELWSATSYKLLREQALDTER
                     WNRLHPGQPARIPLVAELLTEAPGPIVAVTDFMKIVPEQVARFLPGRVFTPLGTDGMG
                     RSDTRESLRRLFEVDAAHVIVAVLSGLAAHGDVKPETVQDAIQRYGIDPEALPATHLP
                     "
     gene            1364..1921
                     /locus_tag="VIX62_11050"
     CDS             1364..1921
                     /locus_tag="VIX62_11050"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEY5860814.1"
                     /translation="MVALDRPRHRWTRTEYALLDSELFPRCELIEGDIIDVAPMSRAH
                     ALVTDQIHELLTRSIDPTVMCAGSQTPIIIGEYSEPEPDVWVARVPRRGLAEGKPLPE
                     ELALVVEVAESSLRVDRRLKLPLYASAGITVTWLVDLAAGRIEIHGEPDAAERRYTTV
                     QLFEEGAAVPLPWSGAVAFDELLLR"
BASE COUNT          344 a          785 c          701 g          345 t
ORIGIN      
        1 cgactcggtc gagtaccagt acatggtcga gcgtcgtcgg gcccagtccg gctcgctgcc
       61 gctccggacg acccgcgtgc ggcggccgct cgggctgccg tccaacgacg cctacgccga
      121 ggtgctgctc ggatcgggca agcaggccgc cagcacgacc accgcgttca cccggctgct
      181 ccgcaacctc tgtcgagacg acaccgtcgg ctctcgcgtc gtgccgatca tccccgacga
      241 ggcccgcacg ttcggcatgg acgcgctctt caaggagctg aagatctacg cctcgcaggg
      301 gcagaagtac cagccgatcg acgcgtcgat gctgctgtcc tacaccgagg cctccgacgg
      361 tcagatcctc gaggaaggca tcaccgaagc gggcgcgatg gccagcttca tcgccgccgg
      421 cacgagctat gccacgcacg gtgtgccgat gatccccttc tacacgttct attcgatgtt
      481 cggtttccag cgggtgggcg acctcatctg gcaggcggcc gacgcccgcg ccagggggtt
      541 cctcatgggc gccaccgccg gccgcacgac gctgctcggc gaaggcctcc agcaccagga
      601 cgggcacagt cacgtgcacg cctccaccgt gccggcgtgc caagcgttcg acccggcgtt
      661 cgcctacgag accgcggtca tcgtcaagga cggcatccgg aggatgttcg ggccgaaccc
      721 cgaggacatc ttctactacc tgaccgtcta caacgagaac taccccatgc cggcgatggc
      781 cgaacgcgac gggatcgaag ccgagatcgt gcgcggcctc taccactggg cgcacgcgcc
      841 cgagggtgtg tcgaagaagg cgacggtgct gttctcgggc tcggcccagg gagcagcgcg
      901 tgaggccgcc gcgcagctcg ccgagcacta cgacgtcggg gtcgagctgt ggtcggccac
      961 ctcgtacaag ctgctccgcg agcaggcgct cgacaccgag cgctggaacc ggctccaccc
     1021 cgggcagccg gcccgcatcc cgctcgtggc cgagctgctg accgaggcac ccggaccgat
     1081 cgtcgccgtc accgacttca tgaagatcgt gcccgagcag gtcgcccgct tcctgcccgg
     1141 acgggtgttc acccccctcg gcaccgacgg catgggtcgc tccgacaccc gcgagtcgtt
     1201 gcgccgattg ttcgaagtgg atgccgccca cgtgatcgtg gccgtgctct cgggcctcgc
     1261 cgcccacggc gacgtgaagc cggagaccgt ccaggacgcc atccagcgct atggcatcga
     1321 ccccgaagcg ctccccgcca cccacctccc ctgagccaca gcagtggtag cgctcgatcg
     1381 tccccgccat cgatggaccc gcaccgagta cgcgctgctc gattcggaac tcttcccgag
     1441 gtgcgaactc attgagggag acatcatcga cgtcgcaccc atgtcgcgcg cccatgcgct
     1501 cgtcaccgac cagatccatg agctactgac gcgatcgatc gatccgactg tgatgtgcgc
     1561 cggatctcag acgccgatca tcatcggtga gtacagcgaa ccggagcccg atgtctgggt
     1621 cgcgcgtgtg ccccgccggg gcctggccga gggcaagcca ctgcccgaag agctcgcgct
     1681 cgtcgtcgag gtggccgagt cgtcgctgcg ggtcgatcga cgcctgaagc tgccgctgta
     1741 cgcctccgcc ggcatcaccg tcacgtggct cgtcgacctg gccgcaggac ggatcgagat
     1801 ccatggcgag ccggacgcgg ctgaacggcg ctacaccacc gtccagctgt tcgaggaggg
     1861 cgcggccgtt cccctgccat ggagtggtgc ggtcgcgttc gacgagttgc tgctgcgctg
     1921 acgttcgcgg cgcagtcaca accgatcact cggctcacga acactgtcgc ggttcccggc
     1981 agttcccggc ggttcccgca attcccggcg gttccccggc agtttcgcgc ctttgtggcg
     2041 tcaggaggcc tcatgggcga cgcctgacgc ctttgtggcg gcagatggtt gggcattcat
     2101 cgccgagcca tcgtgctggc ggccacggcc ataggctcgc gcgcatgaag ggcaccctcg
     2161 ccgtcacggg cgatc
//
LOCUS       DATDBZ010000470         2610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_563559, whole genome shotgun sequence.
ACCESSION   DATDBZ010000470 DATDBZ010000000
VERSION     DATDBZ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563559"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..889)
                     /locus_tag="VIX62_11055"
     CDS             complement(110..889)
                     /locus_tag="VIX62_11055"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY5860815.1"
                     /translation="MTIRTSGHLRGRVVLVTRPADQSASLVNYLDRSGARAIVAPAIE
                     IVPARSAALTTAVRDLSAGRFAWITLTSRATVEMLTTRLPSPEDVRARVAAIGEGTAA
                     AFTAWSGRTPDLVPATFTTTALARAFPRGHGRVLCARADIAPEGLEDALAAKGWTPTR
                     VDAYRTKMPHILPREAREALRDGGVDAVTFTSASTVRGFVGALGAVKGAPKVVCIGPV
                     TAREAREHGLTVHAVAKPHTVEGLVAALERVFTPRASRSVT"
     gene            complement(886..1800)
                     /gene="hemC"
                     /locus_tag="VIX62_11060"
     CDS             complement(886..1800)
                     /gene="hemC"
                     /locus_tag="VIX62_11060"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY5860816.1"
                     /translation="MTALRIGTRRSPLAMAQSQLVQGMLAEVGSASDLVPISTSGDEG
                     AAADTSPQGLKGLWIDTILDALEAGEIDLAVHSAKDLPAEDDEGFTIAAVPPRASPWD
                     VLICRTAGELAPGAVIGTSSLRRRAQLLAAFPGLEMVDLRGNVGTRLDKLAAGEVDAA
                     VLAAAGLDRLGLEPANTSPLPLELMVPAPGQGCLALQCRDDDEETIGLLVAFDDPPSH
                     RALDAERSLMWRLGGGCALPLGAYATIEAEAIHLTCVIATPEGDRVVRVESRGATPED
                     VAAAAAKELIAGGAEEILAELSDETAEL"
     gene            complement(1797..>2610)
                     /locus_tag="VIX62_11065"
     CDS             complement(1797..>2610)
                     /locus_tag="VIX62_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY5860817.1"
                     /translation="ETSVGAAPDAFVAMGADLAQEALGGLSDREVVVVGAGQMASLAV
                     KHLRQRGVGPIRVLNRSLEHARILAQRTGAEPGELDALPEVLRTADLVVSATGAAGAV
                     IDRAAVTDAMAARAERPLVFVDLAVPRDIDPSVASVEHATVFDIAALRDRLAVHDEEA
                     AEDIVRAHGIVADEVHRYVLRRRGDALAPLITALRRRGDEIVTGELDRQASHLGDLTP
                     DERAAVEALARGVAAKLLHDPIVALKERSEPGAEGVHARVLAELLGIDPDEP"
BASE COUNT          390 a          931 c          909 g          380 t
ORIGIN      
        1 gcgaattccc caacgtgatc aggtggctgg tggcccacgg ctacgacgat gagcagattg
       61 cgaagatcgc cggcggcaac gccctgcgcg cgctcgtcga cgtgtgggtc taggtcacgg
      121 accgcgatgc acgaggcgtg aagactcgtt ccagcgcggc caccagacct tcgaccgtgt
      181 ggggcttcgc caccgcgtgc actgtgaggc cgtgctcacg ggcttcgcgt gccgtcacgg
      241 ggccgatgca gaccaccttg ggcgcgccct tgacggcacc gagtgcgccg acgaacccac
      301 gaacggtgga tgcgctcgtg aacgtcaccg cgtcgacccc gccatcacgg agtgcctcgc
      361 gggcttctct cggcaggatg tgcggcatct tcgtgcgata ggcgtcgacg cgcgtcggtg
      421 tccacccttt cgcggcgagc gcatcctcga gtccctcggg tgcgatgtcg gcgcgcgcgc
      481 agagcacacg tccatgccca cgggggaagg cgcgtgcgag ggccgtggtg gtgaacgtcg
      541 ccggtaccag atcgggcgtc cgacccgacc acgccgtgaa tgcggccgcg gtgccctcgc
      601 cgatcgccgc gacccgtgca cgcacgtctt ccggcgaggg cagccgtgtc gtcaacatct
      661 cgaccgtcgc ccggctcgtc agcgtgatcc acgcgaagcg tcctgccgac aggtcacgca
      721 cggcggtcgt gagcgctgca gaacgagctg gcacgatctc gatcgcggga gcgacgatcg
      781 cacgcgcgcc cgaacggtca aggtagttga ccaatgacgc cgattgatct gcggggcggg
      841 tgaccagcac cacgcgacct cgcaggtgac cggacgtgcg gatcgtcaca gctcggcggt
      901 ctcgtcggac agctcggcca ggatctcctc ggcgccgccg gcgatgagtt ccttggcagc
      961 agccgccgcg acgtcctcgg gcgtggctcc ccggctctcc acccgcacca cgcggtcgcc
     1021 ttccggtgtc gcgatcacac aggtcaggtg gatcgcctcg gcctcgatcg tggcgtaggc
     1081 gcccaggggc agcgcgcagc cgccccccaa tcgccacatc aaggaccgtt cggcgtccag
     1141 ggccctgtgg ctcggtgggt catcgaaagc aaccagcagc ccgatcgtct cttcgtcatc
     1201 gtcccggcac tggagcgcga ggcagccttg gccgggtgcc ggcaccatca actcgagcgg
     1261 caatgggctg gtgttcgcgg gttccaggcc cagccggtca aggcccgccg cggcgagcac
     1321 ggcggcatcg acctcgccag cggcgagctt gtccagacgg gtcccgacgt tgccgcgaag
     1381 atccaccatc tccaacccgg ggaacgccgc cagcagctgt gctcggcgcc gcaggctcga
     1441 ggtgccgatc accgcgccgg gggcgagttc gcctgccgtc cggcagatca gcacatccca
     1501 cggtgaggcg cgaggaggga ccgccgcgat cgtgaagccc tcatcgtcct cggcgggcag
     1561 gtccttcgcc gaatgcacgg caaggtcgat ctccccggcc tccagggcat ccaggatcgt
     1621 gtcgatccag aggcctttca acccctgggg ggatgtgtcg gcggcagcgc cttcgtcacc
     1681 ggacgtcgag atcggcacca gatcggacgc cgatcccacc tcggccagca tgccttgcac
     1741 gagctgggac tgcgccatgg cgagcgggga gcgccgggtg ccgatgcgga gcgcggtcac
     1801 ggttcgtcgg ggtcgatccc caggagttcg gccagcacgc gagcgtggac cccttcggcg
     1861 ccgggttcag agcgttcctt caaggccacg atcggatcgt gcagcagctt ggcggccaca
     1921 cccctggcga gcgcttcgac cgcggctcgc tcgtcgggcg tgaggtcacc gaggtgcgag
     1981 gcctgccggt ccaattcccc ggtgacgatc tcgtcgcccc ggcgacgcag cgcggtgatc
     2041 aggggcgcga gcgcgtcgcc ccggcgtcgc agcacgtacc ggtggacctc gtcggccacg
     2101 atcccatggg cgcgcacgat gtcctcggcg gcctcctcat cgtggacggc gagccgatcc
     2161 cggagggcag cgatgtcgaa caccgtggca tgttccaccg acgcgacgct gggatcgatg
     2221 tcccgcggca cggcgaggtc gacgaacacc aggggacgct cggctcgggc ggccatggcg
     2281 tccgtgacgg cagcacgatc gatgaccgcg ccggcggcgc cggtcgcgct gaccaccaga
     2341 tcggcggttc gcaggacctc gggcagcgca tcgagctcgc cgggttcggc acccgtgcgc
     2401 tgggccagga tcctggcgtg ctccagggac cggttcagga cccggatcgg ccccacgcct
     2461 cgttgacgca gatgcttgac cgccagggac gccatctggc ccgcgcccac caccaccacc
     2521 tcgcggtcgg acagaccgcc gagcgcctct tgggcgaggt ctgcgcccat ggcgacgaac
     2581 gcgtcgggcg cggcacccac cgaggtctcc
//
LOCUS       DATDBZ010000471         4332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1800916, whole genome shotgun sequence.
ACCESSION   DATDBZ010000471 DATDBZ010000000
VERSION     DATDBZ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800916"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VIX62_11070"
     CDS             <1..1436
                     /locus_tag="VIX62_11070"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY5860818.1"
                     /translation="VRGRPGPAPGHIVIAFPWRRRAAAEALAAETAGAMGDVLRRSPA
                     RLIAEAGARLEAVDPGPEPTVTDPTIPVIQVTGTNGKTTTVRLLAHLVRTAGRSVAFS
                     STDGVYRDDGELVERGDYSGFGGAAMALAQEPDIAVLETARGGMLLRGAGVLHNDVAV
                     VTNVSADHLGLHGVDTVDQLAEVKGIVTRITRPDGWDVLNADDPRVLAMRRNATGRPW
                     LFSTHPDHPAMREALREGGRAMSIQDGRLTWFSDHRAHPMVAMLDVPMTLAGVSRINA
                     QNAMAAAAAALAIGLPHRAVVKGLKTFVQDPEHNPGRANLFSIEGTVVMIDYAHNEAG
                     MSGLVETLDGLRPRGGHVWLAICTAGDRTNEILRSFAFLAAVGSDHLAVAELPQYLRG
                     RSREDIIERLKDGAEAAGVHEIAVYPDELAALRAMVGEARPGDVVGVTALGMRPEIFA
                     WLEDAGATRMRPADVRRVVRRAQASRA"
     gene            1478..2002
                     /locus_tag="VIX62_11075"
     CDS             1478..2002
                     /locus_tag="VIX62_11075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5860819.1"
                     /translation="MSATIGDFVTDGVSADRLGALYEAHAADALRLAYLLTGDRAVAQ
                     DLVQDAFVKMAGRLAHLRRAEAFGAYLRVTVVNMARMRGRRLKVERDYLARHTAELVE
                     PHRDRDVAATESLRLALATLPFKQRAAIVLRFFEDVPDEVTAQVLGCRQATVRSHIHR
                     GLETLRAELGEWVP"
     gene            1999..3066
                     /locus_tag="VIX62_11080"
     CDS             1999..3066
                     /locus_tag="VIX62_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860820.1"
                     /translation="MTDGFRDELDRLAAGAPTPDERRDHVVRAARRRSRVGLVVASVI
                     AVTGVLVGVLAVGAVLDRRDARPIGPTPTGVVRVSESASTPPPIEARGELAYFRDGPF
                     GGSAQLMVRNADGSDPRTIGDLAISTSGLSVAPDGSQAVFDHGLGEGSGQLEVIDLST
                     GATDPIFSVGQPNGPDWSPDGSTIAFSTDSGALFTIPSGGADSGIPVRDEGDGRFLEG
                     YEASWSPDGDSLVAVDDAGGVFVVEVDGTSPPRRLTSIGRAGWTDWGPDGIVVSTMVN
                     GSWGLYELDPENETAPRPLLDRPGDETAPEVSPDGNFLAFIGIDGEQQDLFVLDLGSD
                     AVDQLTDDAAQELSPAWIPSS"
     gene            3125..>4332
                     /locus_tag="VIX62_11085"
     CDS             3125..>4332
                     /locus_tag="VIX62_11085"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY5860821.1"
                     /translation="MSPNRTRTTVSAMEAPVVVASNRGPVSFDRDDRGRLAARRGSGG
                     LVTALSGVFYRDDTTWVSSAMTDGDRAVALKGRTVAPDSNQRMRYVVMPQERYDGYYN
                     EISNRILWFVHHALWDIPRSPMFDDATEAAWYDYIETNRAFANALADEAEHDPVYLIQ
                     DYHLSLVPGMLRELVPEAKIVHFSHTPFAGATYLRVLPVGMRTALLRGLAGADVLGFQ
                     SKQWAENYLLSARSLPEFHVLRGGRMQIEERDAAVRAFPVAVSAEPLRETAAQPEAES
                     VREELRDLVGERSLILRVDRLEPSKNILRGFLSYELFLRRNPQWRDKCVFLCLFSPSR
                     EEVPEYQDYGEECLAEAKRINAEFGTKSWMPIDIRVQEDYAFAVAAYALYDVLFVNPA
                     YDGMNLVAMEG"
BASE COUNT          635 a         1508 c         1531 g          658 t
ORIGIN      
        1 cggtccgagg ccgccccggg ccggcgcccg gccacatcgt gatcgccttc ccctggcgcc
       61 ggcgcgccgc ggccgaggcg ctggccgccg agaccgcagg agcgatgggc gacgtgctgc
      121 gtcgctcccc cgcccggctg atcgccgagg ccggcgcgag gctcgaggcc gtcgatcccg
      181 gtcctgagcc gacggtgacc gatccgacga tcccggtgat ccaggtgacg ggcacgaacg
      241 gcaaaaccac gacggttcgc ctgctcgccc acctggtgcg gaccgcggga cggtccgtgg
      301 ccttctcctc caccgacggg gtctaccgcg acgacggcga gttggtcgaa cgcggtgact
      361 acagcgggtt cggtggcgcg gcgatggcgc tggcacagga gcccgacatc gcggtgctcg
      421 aaaccgcgcg gggcggcatg ctcctgcgcg gcgcgggggt cctgcacaac gacgtcgccg
      481 tcgtcaccaa cgtcagcgcc gaccacctgg gtctgcacgg cgtcgacacc gtcgatcaac
      541 tcgcggaggt gaaaggcatc gtcacccgca tcacccggcc cgacgggtgg gacgtgctga
      601 acgccgacga tccccgcgtg ctggcgatgc gacgcaacgc gacgggccgt ccgtggctgt
      661 tctcgaccca ccccgatcat ccggcgatgc gtgaggccct gcgcgagggc ggccgcgcga
      721 tgtcgatcca ggacggccgc ctcacctggt tctccgacca tcgcgctcac cccatggtgg
      781 cgatgctgga cgtgcccatg accctggcgg gggtctcccg gatcaacgcg cagaacgcca
      841 tggcggccgc ggcggcggcg ctggcgatcg gcttgccgca tcgggccgtg gtcaagggcc
      901 tgaagacgtt cgtccaggac cccgaacaca accccgggcg agccaacctg ttctcgatcg
      961 aaggcacggt cgtgatgatc gactacgccc acaacgaggc cggcatgagc ggcctggtgg
     1021 agaccttgga cgggcttcgg ccgcgaggcg gccacgtctg gctcgcgatc tgcacggcgg
     1081 gcgaccggac gaacgagatc ctgcggtcgt tcgcgttcct ggccgcggtg ggttcggacc
     1141 acctggcggt cgccgagctc ccgcagtacc tgcgcgggcg ctcgcgcgaa gacatcatcg
     1201 agcgactgaa ggacggcgcg gaggcagcgg gcgtgcacga gatcgccgtc taccccgatg
     1261 aactcgccgc gctgcgcgcg atggtgggcg aagctcgccc tggcgatgtc gtgggcgtca
     1321 ccgctctggg gatgcgcccg gagatcttcg cctggctcga agacgccgga gccacgcgga
     1381 tgaggcccgc cgacgtccgg cgggtggtgc gccgggcgca ggcttcccgt gcgtgagtgc
     1441 aacgcgggag ccgctcggag catccaggtt cgagtgtatg agcgcgacga tcggtgactt
     1501 cgtgaccgac ggcgtgagcg cggaccggct cggcgccctg tacgaggcgc acgcggccga
     1561 cgccttgcgc ctcgcctacc tgctcaccgg cgaccgcgcg gtcgctcagg acctggtcca
     1621 ggacgccttc gtgaagatgg cgggcagact cgcccacctg cgccgtgccg aggcgttcgg
     1681 cgcctacctc cgcgtcaccg tggtgaacat ggcacggatg cgcgggcgtc ggctcaaggt
     1741 ggagcgcgac tacctcgctc gtcacacggc cgaactggtc gagcctcatc gagaccgcga
     1801 cgttgccgcc accgagagtc tgcggctggc gctggccacc ctgccgttca agcagcgcgc
     1861 ggcgatcgtt ttgcggttct tcgaggacgt gccggacgag gtcaccgccc aggtgctcgg
     1921 gtgccggcag gcgaccgtgc gctcgcacat ccaccgaggg ctggagaccc tgcgtgccga
     1981 gctcggagag tgggtgccgt gaccgacgga ttccgcgacg aactcgaccg cctcgcggcc
     2041 ggagcgccga ccccggacga acggcgcgac cacgtggtgc gcgccgcccg acgccgcagc
     2101 cgggtgggct tggtcgtggc gagcgtgatc gcggtcaccg gtgtcctcgt cggggtgctg
     2161 gccgtgggcg cggtgctcga ccgccgggat gcgcggccca tcggaccgac gccgacgggg
     2221 gtcgtgcggg tgtccgagtc ggcatccacg cccccaccga tcgaagcccg gggcgagctc
     2281 gcttacttcc gggacggacc gttcggtggc tccgcccagt tgatggttcg aaacgcagac
     2341 ggatcggatc cgcggacgat cggtgatctt gcgatctcga cgtccgggct gtcggtcgcg
     2401 cccgatggct cgcaggcggt cttcgatcac gggctgggcg agggctcggg gcagctcgag
     2461 gtgatcgacc tgtcgaccgg agcgaccgac ccgatcttct cggtgggcca gccgaacggc
     2521 cccgactggt cgcccgatgg atcgacgatc gcgttctcca ccgattccgg agcgctcttc
     2581 acgatcccgt ccggtggagc cgactcgggc atcccggtcc gagacgaggg cgacggacgg
     2641 ttcctcgagg gctacgaggc cagctggtct ccggacggtg attcgctcgt ggccgtcgac
     2701 gatgccggcg gtgtcttcgt cgtggaggtc gacggcacct cccccccacg acgcctcacg
     2761 tcgatcggtc gtgcgggctg gacggactgg ggccccgacg ggatcgtggt ctcgaccatg
     2821 gtgaacggct cctgggggct ctacgaactc gacccggaga acgagaccgc tccacggccg
     2881 ttgctcgacc gtcccggtga cgaaaccgcc ccggaggtct cccccgatgg caacttcctc
     2941 gccttcatcg gcatcgacgg ggagcagcag gatctgttcg tcttggatct gggttcggac
     3001 gccgtggatc agctgaccga cgatgcggcc caggagctct cgccggcgtg gatcccctcg
     3061 tcctgacgcc gtaggtgtac gggcccgtac cgagggttcg gccggaccac ctagactgcg
     3121 ggggatgtca cccaacagaa cccgcaccac cgtcagcgcc atggaggcgc cggtggtggt
     3181 ggcgtccaac cgagggcccg tcagcttcga tcgggacgat cgaggcaggt tggcggcacg
     3241 gcgtggctcc ggcgggctgg tcacggcgct gtcgggggtg ttctaccggg acgacaccac
     3301 ctgggtgtcc tcggccatga ccgacggcga tcgcgcggtg gccctgaaag gccggacggt
     3361 ggctcccgat tcgaaccagc gcatgcgcta cgtcgtgatg ccgcaggagc gctacgacgg
     3421 ctactacaac gagatctcga accggatcct gtggttcgtc caccacgcgc tgtgggacat
     3481 cccccgctcg ccgatgttcg acgacgccac cgaggccgct tggtacgact acatcgagac
     3541 caaccgcgcg ttcgccaacg ccctcgccga cgaagccgag cacgaccccg tgtacctgat
     3601 ccaggactac cacctgagcc tggtgcccgg catgctgcgt gaactcgtgc ccgaggccaa
     3661 gatcgtgcac ttctcgcaca cgcccttcgc cggtgccacg tatctccggg tcctgccggt
     3721 cggcatgcgg accgcgctgc tccggggact cgccggcgcc gacgtcctgg gcttccagtc
     3781 caagcagtgg gccgagaact acctgctgtc ggcgcggagc ctgcccgagt tccatgtgct
     3841 ccgtggcggc cgcatgcaga tcgaggaacg cgacgccgcg gtgcgcgcct tccccgtggc
     3901 ggtgagcgcc gagccgctgc gcgagacggc ggcccagccc gaggccgaga gcgtgcgcga
     3961 ggagctccgc gacctggtgg gtgagcgttc cctgatcctg cgtgtcgacc ggctcgaacc
     4021 gtcgaagaac atccttcgag gcttcctgtc ctacgagctc ttcctgcgaa ggaaccccca
     4081 gtggcgcgac aagtgcgtct tcctctgcct gttcagcccg tcacgcgagg aggtgcccga
     4141 gtaccaggac tacggcgagg aatgtctcgc cgaggccaag cgcatcaacg ccgagttcgg
     4201 cacgaagtcc tggatgccga tcgacatccg ggtgcaggag gactacgcct tcgccgtggc
     4261 cgcctacgcc ctgtacgacg tgctgttcgt caacccggcc tacgacggca tgaacctggt
     4321 cgccatggag gg
//
LOCUS       DATDBZ010000472         2248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1600562, whole genome shotgun sequence.
ACCESSION   DATDBZ010000472 DATDBZ010000000
VERSION     DATDBZ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600562"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VIX62_11090"
     CDS             complement(<1..838)
                     /locus_tag="VIX62_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5860822.1"
                     /translation="MIDTWTARIGRIGADPRDDEGLRQKKALLVLLAVLILPVSLAWG
                     ASYLAFGSRVGILPFVYFAISVGSLVVFSRTRKFGPFLTVQLLDVLLMTTLGQMVSGG
                     FLPSGGVGLWGMLAPLGALVFLEVRQAIGWFVGFVAAFVLLGVGGEMFFTDIDVPTWF
                     TTTMLALNVIGTGAVAFTVLASFATQRNAALTALRAEQARSEALLTNVLPSAIAERLK
                     AGSGSIADHVESATIVFADVVDFTPLSQRLTPAEVVGTLDELFSRFDTLVERHGLEKI
                     KTI"
     gene            complement(835..1389)
                     /locus_tag="VIX62_11095"
     CDS             complement(835..1389)
                     /locus_tag="VIX62_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5860823.1"
                     /translation="MGKLIVMNQISVNGAFEAPTPDDWLVLDEDSSEASLEQLILADA
                     MVLGRKTYEGLAAVWPQLADVPAMARFAERLNPMPKYVASRTLTEPLEWNARLITGDL
                     EEAIPALKAEHQGNLIVSGCGELTHSLTRQGSVDEFWFWINPFIWADGPRIFDRVGPV
                     RLELVASTPYRSGVVWLRYRPAAG"
     gene            complement(1449..1925)
                     /locus_tag="VIX62_11100"
     CDS             complement(1449..1925)
                     /locus_tag="VIX62_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860824.1"
                     /translation="MSGEEWALAAAVVFAGLWSGLLGMLTLVMHKMLGALDGPGFLGF
                     MSAFLPVGRKAWFNYVCAIGMAVAPIVALVLLWDDPGSAAFVLTAIGLGFVIVGVYIV
                     SNVWKEPHYDVMLAWDPEAMPADWKAGRQKYFTINWIQLVSTWAAFGLFLAALISL"
BASE COUNT          406 a          771 c          742 g          329 t
ORIGIN      
        1 cgatcgtctt gatcttctcg agcccgtgtc gttcgacgag cgtgtcgaac cgcgagaaca
       61 actcgtccag cgtccccact acctcggccg gcgtaaggcg ctgggagagc ggcgtgaaat
      121 ccacgacgtc ggcgaacacg atcgtggccg actcgacgtg gtcggcgatc gatcccgagc
      181 cggccttcag tcgctccgcg atcgcgctcg gcagcacgtt cgtgagcagc gcctcggacc
      241 gcgcctgctc ggcccgcagc gctgtcagcg ccgcgttgcg ctgggtggcg aacgacgcga
      301 gcaccgtgaa cgcgaccgca ccggtgccga tcacgttgag cgccagcatc gtggtcgtga
      361 accacgtcgg cacgtcgatg tcggtgaaga acatctcgcc cccgacgccc aggagcacga
      421 aggccgccac gaacccgacg aaccagccga tggcctggcg aacctcgagg aacacgagcg
      481 cgccgagtgg ggccagcatg ccccagagcc cgacccctcc cgagggaagg aatccgcccg
      541 aaaccatctg cccgagcgtc gtcatgagga gcacgtcaag gagctgcacg gtgaggaacg
      601 gcccgaattt cctggtcctc gagaacacca ccaacgagcc gaccgagatc gcgaagtaga
      661 cgaacggaag gatgccgacc cgcgagccga aggcgaggta cgaagcgccc cacgccaggg
      721 acacgggcaa gatcagcacc gcgagcagca cgaggagagc cttcttctgg cgcaggccct
      781 cgtcgtccct ggggtccgcg ccgattcggc cgatccgcgc ggtccaggtg tcgatcaccc
      841 ggctgccggg cggtagcgca gccacacgac gcccgatcgg tacggggtcg acgcgacgag
      901 ctcgagtcga acgggcccca cgcggtcgaa gatcctcgga ccgtcggccc agatgaacgg
      961 gttgatccag aaccagaact cgtcgaccga gccctggcgg gtcagcgagt gcgtgagctc
     1021 gccgcaccca gacacgatca ggttcccctg gtgctccgcc ttgagggcgg ggatggcctc
     1081 ctcgagatcg cccgtgatca gcctggcgtt ccactccagc ggctcggtca gcgtccgcga
     1141 cgcgacgtac ttcggcatcg ggttcaggcg ctcggcgaag cgagccatcg ccggaacgtc
     1201 ggcgagctgc ggccacacgg ccgccagccc ttcgtacgtc ttgcgtccga ggaccatcgc
     1261 gtcggcgagg atgagctgtt cgagcgaggc ctcgctgctg tcttcgtcga gaacgagcca
     1321 atcgtcgggc gtcggcgcct cgaaggctcc gttgacgctg atctggttca tcacgatgag
     1381 cttgcccatc agccacccct ccctgctccc ggaaccacgg gtcccgagga taggggagcc
     1441 gtaccccgct agagcgagat caacgccgcg aggaagagcc cgaacgcggc ccaggtcgag
     1501 acgagctgga tccagttgat cgtgaagtac ttctggcgtc cggccttcca gtcggcgggc
     1561 atggcctcgg ggtcccaggc cagcatcacg tcgtagtgcg gttccttcca cacgttcgag
     1621 acgatgtaca cgccgacgat cacgaacccc agcccgatcg cggtgagcac gaacgcggcg
     1681 gagccgggat cgtcccagag gagcacgagc gccacgatcg gcgcgacggc catcccgatc
     1741 gcacagacgt agttgaacca ggccttcctg ccgaccggca ggaacgcgct catgaagccc
     1801 aggaaacccg ggccgtccag cgctccgagc atcttgtgca tcaccagggt cagcatgccc
     1861 aacaggcccg accagagtcc cgcgaacacg accgccgccg cgagtgccca ctcctctcca
     1921 gacatgggtg gaacctcccc ccttcacgtt gcgcgccagc ctatgcggat gttcccgcgg
     1981 ttgtgaaggt ggcgatggcg tcgacaggat gggcgcgcgt cctcgatgct tggggggtcg
     2041 agccgatttc ggatgccagg gggcaatcgc gaccacgttt gtcttgatcc acggtgcggg
     2101 cgactcggga tggtactggc atctcgtgga ggaagagctc cgtgccctcg gtcacgactc
     2161 cgtcgcgccc gatctgccgg tcgaaggctc gccgacgttg gtcgagtgcg ccgacgcggt
     2221 ggtgcaggcc gtcgggaacc cgtcggca
//
LOCUS       DATDBZ010000473         4254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_784056, whole genome shotgun sequence.
ACCESSION   DATDBZ010000473 DATDBZ010000000
VERSION     DATDBZ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784056"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VIX62_11105"
     CDS             complement(<1..1026)
                     /locus_tag="VIX62_11105"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY5860825.1"
                     /translation="MTARAAASTGRRERPASPELRRDVRLLTTMLGDAIAESGGPALL
                     AEVEALRLATIAFRGTPTAARRATIVSLVDQLDVTRAEDVIRAFTCYFQLVNLAEERQ
                     RLRVLARRARSGKPVKDSIDALDVDASAFEDLRITPVLTAHPTEAKRRAVVEHLWRIG
                     TLLEHLEDLPTGAADEQEIHRRLREEIAGLWRTEPIRRHRPEPLDEVRATLALFDQTI
                     FVTLPAIYRAVDRTLDPERCGARPPSFAPFLRWGTWVGGDRDGNPAVTAEVTRAAMAI
                     QTDHALRGMETAARRIARTLSVSERDVPPSRALRRALDRDAGELPEVATELGRTLSDA
                     PHRRKLGL"
     gene            complement(1034..2020)
                     /locus_tag="VIX62_11110"
     CDS             complement(1034..2020)
                     /locus_tag="VIX62_11110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY5860826.1"
                     /translation="MSERPNILVTRPIMDGATATLRERADVTIHENEFGIPRDELLEV
                     IAGRDAIITMLTEQVDAEFLAAAGPNLTIVANHAVGFDNIDVAACTAAGVLATNTPDV
                     LTETTADTAFALLMAAARRLGEGERFLRAERPWIWGPLMMLGQDVHHKTIGIVGFGRI
                     GQAVARRAKGFGMDVIYADAFQLPADVEAESGARRVELEALLEQADFVSIHTNLTEET
                     RHLFDAETFKRMKPTAVLVNTSRGPVVNESALADALESGEIFGAGLDVFEREPQVEPR
                     LLALENVVVIPHLGSATVDTRDAMGALAVRNVFAALDGTRPPTLLNPDAFKE"
     gene            2125..2643
                     /locus_tag="VIX62_11115"
     CDS             2125..2643
                     /locus_tag="VIX62_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860827.1"
                     /translation="MSPPTLETERLRLRPYAADGSDLDALQAVLGDPVSMRWYPAPFD
                     RAATARWIDRQLERYVADGTGLLAVEERATGEVIGDCGPSFQDVDGESLLELGWHIRL
                     DRQGRGFATEAGAACRDWAFAELQPAFLISLIRPENIGSWRVAEKLGFEIWRGTIRAG
                     WGHLVYRLDPPA"
     gene            complement(2679..3311)
                     /locus_tag="VIX62_11120"
     CDS             complement(2679..3311)
                     /locus_tag="VIX62_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY5860828.1"
                     /translation="MTAVLCYGDSNTWGYAASTQQRLGRWERWPGVVQRALGEDVHVI
                     EEALNGRTTAFEVPFDPGRNGLAYLPIALESHAPLDVVVIALGVNDLFVPGALSVHHA
                     ARGAITLAEVVATSGTGPGGEAPEVLLLVPPPLAALGVWDAESPHAELESRGFADAYR
                     LVAEDAQVPLLDLGAFVTSSSVDGVHFEAEDHRVMGEAIAAALRDLMELR"
     gene            complement(3308..3817)
                     /locus_tag="VIX62_11125"
     CDS             complement(3308..3817)
                     /locus_tag="VIX62_11125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010350709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5860829.1"
                     /translation="MPNATIVTNQGSFTVTLMPEHAPKTVQNFVDLATGGREWTDPRD
                     GQTKSDALYPGTVFHRVIDGFMVQGGDPTGTGMGGPGYTFEDECPPDGPTFNQVGRLA
                     MANAGPGTNGSQFFVTVAETPWLNGRHTIFGQVTEGYDVVEAMSKVATSGQDKPADEI
                     VIERIDIAE"
     gene            complement(3892..>4254)
                     /locus_tag="VIX62_11130"
     CDS             complement(3892..>4254)
                     /locus_tag="VIX62_11130"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5860830.1"
                     /translation="SRSADEGGAPVASMVFDHEGGGTIWEVRADGGRASWGQILAWEP
                     PTRLVMAWKPHDRPVGPTEVEVTFTALEEGGTRVDLEHRGWERLGDLALEGRENYASG
                     WPMVFDQRFADAANGVAA"
BASE COUNT          599 a         1507 c         1505 g          643 t
ORIGIN      
        1 caggcccagc ttccgccggt ggggcgcgtc ggacagcgtc cgcccgagtt cggtggcgac
       61 ctcggggagt tcgcccgcgt cccgatccaa cgcccggcgc agcgcgcggc tcggcggcac
      121 gtcgcgctcg gacaccgaca gcgtccgggc gatccgacgg gcagccgtct ccatcccccg
      181 gagcgcatgg tcggtctgga tcgccatcgc ggcacgcgtg acctcggcgg tgacggcggg
      241 gttgccgtcg cgatccccac ccacccaggt accccatcgg aggaacggcg cgaacgaggg
      301 cggtcgcgct ccacaacgtt cggggtcgag ggtacggtcc accgcgcgat agatcgccgg
      361 cagggtcacg aagatcgtct gatcgaacag ggcgagcgtc gcgcgcacct catcgagcgg
      421 ttcgggacgg tgccggcgga tcggctccgt acgccacagc ccggcgatct cctcgcgcag
      481 ccggcgatgg atctcctgct cgtcggcggc tcccgtgggc agatcctcga ggtgttcgag
      541 cagggtgcct atccgccaca ggtgttcgac cacggcgcga cgcttggcct cggtggggtg
      601 ggcggtcaac accggggtga tgcgcagatc ctcgaacgcg gacgcgtcca cgtccagggc
      661 gtcgatcgag tccttcaccg gcttgcccga acgagcgcga cgtgccagga cgcgcagacg
      721 ctggcgttcc tcggccaggt tcacgagctg gaagtaacag gtgaacgcgc ggatcacgtc
      781 ttcggcgcgt gtcacatcga gctgatccac gagggagacg atcgtggccc ggcgggctgc
      841 cgtgggcgtg ccccggaacg cgatcgtcgc gaggcggagc gcctccacct cggcgagcag
      901 cgcggggccg ccgctctcgg cgatcgcgtc gccgagcatc gtggtgagca ggcgaacgtc
      961 acgccggagc tcaggggagg cgggccgctc tcggcggccc gtcgaagcgg ccgcacgggc
     1021 ggtcacgccg tcgtcactcc ttgaacgcgt cgggattcag cagtgtaggc ggccgcgtgc
     1081 cgtccagcgc cgcgaatacg ttccgcaccg ccagcgcgcc catcgcatcg cgggtgtcca
     1141 cggtggcact gccgaggtgc gggatcacga cgacgttctc cagggccaac aggcggggct
     1201 cgacctgtgg ctcgcgctcg aacacatcga gccccgcgcc gaagatctcg ccggactcca
     1261 gggcatcggc cagcgccgat tcgttgacga caggcccgcg ggaggtgttg accaacacgg
     1321 cggtcggctt catgcgcttg aacgtttcgg cgtcgaacag atgccgcgtc tcctccgtga
     1381 ggttcgtgtg gatcgagacg aagtcggcct gctccaggag cgcctcgagt tccacgcgcc
     1441 gggcgccgga ctcggcttcg acgtcggcgg ggagctggaa cgcgtcggcg tagatcacgt
     1501 ccatcccgaa gcccttcgcg cgccgggcca ccgcctgccc gatccggccg aaccccacga
     1561 tgccgatcgt cttgtggtgc acgtcctggc ccagcatcat cagcggaccc cagatccagg
     1621 gccgctccgc gcgcaggaag cgttcccctt cgcccaggcg ccgcgcggcc gccatcagca
     1681 gcgcgaacgc ggtatcggcg gtggtctcgg tgagcacgtc gggcgtattc gtggccagca
     1741 cgccggcggc ggtgcacgcg gcaacgtcga tgttgtcgaa gcccacggcg tggttcgcga
     1801 cgatcgtcag gttcgggcct gctgcggcca ggaactcggc gtcgacctgc tcggtgagca
     1861 tcgtgatgat ggcgtcgcgg ccggcgatca cttcgaggag ctcgtcccgc gggatgccga
     1921 actcgttctc gtggatcgtg acgtcggcgc gttcgcgcag ggtcgccgtc gccccgtcca
     1981 tgatcggccg ggtcaccagg atgttcggtc gttcgctcat gaggaggatc cttccgtgcg
     2041 cgtggagagc ccgaagtatg gaccgaaccg ccgggggagc ggtaaccgcg agccgtgacc
     2101 acggcatcta gactcctccg tcggatgagt ccgcccaccc tggagaccga gcgtctgcgc
     2161 ctgcgcccgt acgcggccga cggctccgac ctcgatgccc tgcaggcggt cctgggtgac
     2221 cccgtgtcga tgcggtggta cccggcgccg ttcgatcgcg cggccaccgc ccgatggatc
     2281 gaccgccagc tcgagcggta cgtggcggac ggcaccgggc tcctcgcggt ggaggaacgc
     2341 gcgaccggcg aggtgatcgg cgactgcggg ccctcgttcc aggacgtcga cggcgagtcg
     2401 ttgctggagc tgggctggca catccgcctc gaccggcagg ggcgggggtt cgccaccgag
     2461 gccggtgccg cctgccgcga ctgggcgttc gccgaactgc agccggcctt cctcatctcg
     2521 ttgatccgcc ccgagaacat cggctcgtgg cgagtggccg agaagttggg gttcgagatc
     2581 tggcgcggca cgatccgcgc cggctgggga cacctcgtgt accggctcga cccgccggcg
     2641 tgactcgccg cgtgaggcgg gccaacgacg acggcgcgct agcgcagctc catcaggtcg
     2701 cgcagcgccg ccgcgatcgc ctcgcccatc acgcggtggt cctcggcctc gaaatgcacg
     2761 ccgtccaccg agctcgaggt cacgaacgcc ccgaggtcga gcagcggcac ctgggcgtct
     2821 tcggccacga ggcggtaggc gtcggcgaac ccgcgggact ccagctccgc gtgcgggctc
     2881 tcggcgtccc acaccccgag ggcagcgagc gggggcggca ccagcagtag cacctcgggc
     2941 gcctcgccgc caggcccggt gccgctcgtt gccaccacct cggccagggt gatcgcgcct
     3001 cgcgccgcat ggtgcaccga gagcgccccc ggcacgaaca ggtcgttcac gcccagcgcg
     3061 atcaccacga cgtcgagggg cgcgtgactc tccagcgcga tcggcagata cgccagcccg
     3121 ttgcgccccg gatcgaacgg cacctcgaac gccgtcgtgc ggccgttgag ggcctcctcg
     3181 atcacgtgga cgtcctcacc gagcgcgcgt tgcacgacgc cgggccaccg ctcccatcgg
     3241 cccaggcgct gctgggtcga ggcggcgtag ccccaggtgt tggagtcccc gtaacagagg
     3301 acggcggtca ctcggcgatg tcgatgcgct cgatcacgat ctcgtcggcg ggcttgtcct
     3361 ggccgctggt ggccaccttc gacatcgctt ccaccacgtc gtagccctcg gtgacctgcc
     3421 cgaagatcgt gtgtcgcccg ttcaaccacg gcgtctcggc cacggtcacg aagaactgcg
     3481 aaccgttcgt gccgggtccg gcgttggcca tcgcgagccg acccacctgg ttgaacgtgg
     3541 gaccgtcggg cgggcattcg tcctcgaacg tgtagccggg accgcccatg cccgtgcccg
     3601 tggggtcgcc gccctgcacc atgaacccgt cgatcacccg gtggaacacg gtgcccggat
     3661 acaaggcatc ggacttggtc tggccgtccc gcgggtcggt ccactcacgt ccgccggtgg
     3721 cgagatcgac gaagttctgc acggtcttcg gggcgtgctc gggcatcagg gtgacggtga
     3781 acgaaccctg gttcgtgacg atggtggcgt tgggcatggg ccagcctcct ggaggtcggg
     3841 aagcggccag tctaccgggc gcggatcgag cgatctgcgt gggcgtgccc ctcaggcggc
     3901 caccccgttc gcggcgtcgg cgaagcgctg atcgaagacc atcggccacc cgctcgcata
     3961 gttctcgcgg ccctcgagcg cgaggtcgcc gaggcgttcc cagcctcggt gttcgaggtc
     4021 gacccgtgtc ccgccctcct cgagcgccgt gaacgtcacc tcgacctcgg tgggtccgac
     4081 ggggcggtcg tgcggtttcc acgccatgac cagccgggta ggtggttccc aggcgaggat
     4141 ctggccccac gatgcccgcc caccgtcggc gcgcacctcc cagatcgtgc cgcccccctc
     4201 gtggtcgaac accatggagg ccacgggggc gccgccttca tcggccgatc ggga
//
LOCUS       DATDBZ010000474         2826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_784249, whole genome shotgun sequence.
ACCESSION   DATDBZ010000474 DATDBZ010000000
VERSION     DATDBZ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784249"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..262
                     /locus_tag="VIX62_11135"
     CDS             17..262
                     /locus_tag="VIX62_11135"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY5860831.1"
                     /translation="MLVADWLALLRSDPRLPRHFLGADWPADRSFEVYWRWRRRLDAA
                     STAQFDAIVATSEVSPRTALEDPPSAPSRTPSAAARR"
     gene            complement(189..1697)
                     /locus_tag="VIX62_11140"
     CDS             complement(189..1697)
                     /locus_tag="VIX62_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY5860832.1"
                     /translation="MHNHGLGAWMAKRRLKTPDKPALIHGDDETVTYRQLADGTDRVS
                     ALLWHRGVRKGDRVAFLGENSPEFLQVLFGAAQLGAVFVPINTRLAPPEIAHVLTDSG
                     TRVLIHDPEFAERVAEGVAQGGVAHVIVTGAGGLNGLLAAASGGHADADVTLDDPAAI
                     LYTSGTTGRPKGAVLTHQNLTWVALNCLVDYDVVSTDVALMISPLFHAASLGMGALPV
                     ILKGATIVLEKGFEPGRALELIERHGVTMLSGVPTTYQLLADHPRWATTDLSTLQKLT
                     CGGSAVPTRILNAYEERGLSFSQGYGMTEASPGATSLAPAMTRAKQGSVGLAHFFTDV
                     RIADERGAMVPRGTVGEIEITGPNVFPGYHNLPEATAAAFSPDGWFRSGDMGYLDPDG
                     YLYIADRLKDMIISGGENIYPAEIENLISDIDGVTGVAVIGVPHDTWGEVPWAVLTVR
                     EGGEVSLDDVRARLEGRIARFKIPKNVVVIDELPRTASGKVRKADLRARFGG"
     gene            complement(1769..2221)
                     /locus_tag="VIX62_11145"
     CDS             complement(1769..2221)
                     /locus_tag="VIX62_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY5860833.1"
                     /translation="MTTTIAYTDVAGLAGTDLGYTDWVEITQQQIGTFADATGDHQWI
                     HVDPERAKDGPFGAPIAHGFLTLSLAVTFWTELLEVEGVTTKVNYGLDKVRFVSPVKV
                     GARVRMNAVVAEVTEIAGGYQLTVDQTIEIDGATKPAVVARGLYRFYA"
     gene            complement(2266..>2826)
                     /locus_tag="VIX62_11150"
     CDS             complement(2266..>2826)
                     /locus_tag="VIX62_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5860834.1"
                     /translation="IRGKVSLRPMQNATITLAGVRVPESARLQRVESWRDVARLLRAM
                     RSDVAWIATGLQAGSLDAALAYVRTREQFGRPIGGFQLVQEKLARMLGNVTASLALVV
                     QLSTRQDAGDDSDENSALAKMWTSLRARETVALAREVVGGNGILLEHDVARFFADAEA
                     VYSYEGTHEINSLIVGRSLTGVSAFT"
BASE COUNT          419 a          938 c         1009 g          460 t
ORIGIN      
        1 actggctgca cgcacgatgc tcgtcgccga ctggctcgcc ctgctgcgga gcgatcctcg
       61 cctgccacgg cacttcctcg gagcggactg gcctgccgac cggtcgttcg aggtctactg
      121 gcgctggcgc aggcgattgg atgcggcatc caccgcccag ttcgacgcga tcgtcgcgac
      181 gtccgaagtc agcccccgaa ccgcgctcga agatccgcct tccgcacctt cccggacgcc
      241 gtccgcggca gctcgtcgat gacgacgacg ttcttcggga tcttgaagcg tgcgatccgt
      301 ccctccaggc gagcgcggac atcgtccagc gagacctcgc ctccctcgcg caccgtgagc
      361 accgcccacg gcacctcgcc ccacgtgtcg tgcggcacgc cgatcacggc gaccccggtc
      421 acgccgtcga tgtcgctgat gaggttctcg atctcggcgg gatagatgtt ctcgccgccc
      481 gagatgatca tgtccttgag gcggtcggcg atgtagaggt agccgtcggg gtcgaggtag
      541 cccatgtcgc ccgagcggaa ccagccgtcg ggtgagaacg ccgccgcggt ggcctcaggc
      601 aggttgtgat accccgggaa cacgttcggc ccggtgatct cgatctcgcc caccgtgccg
      661 cgcggcacca tcgccccgcg ctcgtcggcg atgcgcacgt cggtgaagaa gtgcgcgagg
      721 ccgacgctgc cctgcttggc gcgcgtcatg gccggagcga gcgaggtcgc gccgggagag
      781 gcctcggtca tcccgtaccc ctgcgagaac gacagaccgc gctcctcgta ggcgttgaga
      841 atgcgggtgg gaaccgccga gcccccgcac gtgagtttct gcagggtgct gaggtcggtg
      901 gtcgcccacc gcggatggtc cgcgaggagc tgatacgtcg tgggtacgcc gctcagcatc
      961 gtgacgccgt gacgctcgat gagctcgagg gcccgcccgg gctcgaaccc cttctcgagc
     1021 acgatcgtgg cccccttcag gatcaccggc agcgcgccca tgccgaggga ggcggcgtgg
     1081 aagaggggcg agatcatcag ggcgacgtcg gtggagacca cgtcgtagtc gaccaggcag
     1141 ttcagtgcga cccacgtgag gttctggtgg gtgagcacgg ctcccttcgg cctgccggtc
     1201 gtccccgagg tgtagaggat cgcggccggg tcgtcgagcg tgacgtcggc gtcggcgtgc
     1261 ccgccggatg ctgcggcgag cagaccgttc agtccgcccg caccggtgac gatcacgtgc
     1321 gccacgccac cctgcgcgac cccctcggcc acgcgctcgg cgaactcggg atcgtggatg
     1381 agcacgcgcg tgccggagtc cgtgagcaca tgggcgatct cgggcggagc gagtcgcgtg
     1441 ttgatcggca cgaacacggc accgagctgg gccgcgccga agagcacctg caggaactcg
     1501 ggactgttct cgcccaggaa cgccacccgg tcgcccttgc gcactccccg atgccacagc
     1561 agtgccgaga cgcggtcggt gccgtcggcg agctgccgat acgtgaccgt ctcatcatcg
     1621 ccatggatca gtgccggttt gtcgggggtc ttcaggcgcc gtttggccat ccatgcgccc
     1681 agtccgtggt tgtgcatcgg tgatctcttc gtcgaggtgg ggcggtggtg ccgggatgcc
     1741 gccgcagcgg catcccggag tgtgaggtct acgcgtagaa gcggtagagc ccgcgggcga
     1801 cgacggccgg cttggtggcg ccgtcgatct cgatggtctg gtcgaccgtc agctggtagc
     1861 cgcccgcgat ctcggtgacc tcggcgacga ccgcgttcat gcgcactcgc gcaccgacct
     1921 tgaccggcga gacgaagcgc accttgtcga gcccgtagtt gaccttcgtg gtcacaccct
     1981 cgacctcgag aagctcggtc cagaaggtga cggcgagcga cagagtgagg aacccgtggg
     2041 cgatgggtgc gccgaagggt ccgtccttcg cgcgctcggg gtcgacgtgg atccactggt
     2101 ggtcgccggt tgcgtcggcg aacgtgccta tctgctgctg ggtgatctcg acccaatcgg
     2161 tgtagccgag gtcggtgccg gcgagtccgg cgacgtcggt gtaggcgatc gtggtggtca
     2221 tgggtgctcc ttcggtggat ggaaacgcgg tgaggcccgc gagggtcagg tgaaagcgga
     2281 cactccggtg agcgaccggc cgacgatgag ggagttgatc tcgtgcgtgc cctcatagga
     2341 gtagacggct tcggcatcgg cgaagaaccg ggcgacgtca tgctcgagga ggatgccgtt
     2401 gccgcccacc acctcccggg cgagggcgac ggtctcgcgt gcgcgcagcg aggtccacat
     2461 cttcgcgagt gcggagttct cgtcggagtc gtcaccggcg tcctgtcggg tggagagctg
     2521 cacaaccagc gctagtgagg cggtgacgtt gcccagcatg cgggcgagct tctcctgcac
     2581 gagctggaag ccgccgatcg ggcgcccgaa ctgctcgcgc gtgcgcacgt aggcgagcgc
     2641 cgcgtcgagg gacccggcct gcagccccgt tgcgatccac gcgacgtcgg agcgcatggc
     2701 ccggagcagc cgggccacat cgcgccacga ctccacacgc tgcaggcgcg cgctctcggg
     2761 cacgcgcacg ccggcgaggg tgatcgtggc gttctgcatc ggacgcagtg acaccttgcc
     2821 gcggat
//
LOCUS       DATDBZ010000475         2392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_785203, whole genome shotgun sequence.
ACCESSION   DATDBZ010000475 DATDBZ010000000
VERSION     DATDBZ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785203"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..>2392)
                     /gene="mfd"
                     /locus_tag="VIX62_11155"
     CDS             complement(217..>2392)
                     /gene="mfd"
                     /locus_tag="VIX62_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY5860835.1"
                     /translation="AKEVVGAVDDAVESPLASGFVFEAAKLAVATEEDLFGSRRHTRT
                     APRFTRRKTESIADELEPGDFAVHRIHGVGRYVGITHRELAGAERDYLILEYAQGDKL
                     FVPSDQVGMVARYIGGDAPRLHRMGGSDWARATTKVKRAVRDMAGELVRLYTVRMSVP
                     GHAFGPDTPWQQELEDAFPHEETSDQVTAIDEVKADMELAKPMDRLICGDVGFGKTEI
                     AVRAAFKAVMEGKQVAVLVPTTLLAEQHFITFSERFAPFPVTVRMLSRFVSPAQQAET
                     VQDAKDGKVDIVIGTHRLLGKDMAFKDLGLLVVDEEQRFGVAHKERLKQFRASVDVLT
                     MTATPIPRTLEMALTGIREMSTIDTPPEDRQPVLTYVGAYDEGLSLGAVRRELLREGQ
                     VFWVHNRVATIDRHAAWLQEQLPDARVVTAHGQMDEDLLEQQMMRFWNREADVLVSTV
                     IIESGLDVPNANTLIVDRADALGLSQMYQLRGRVGRSTERAFAYFFFAPQREMTDEAR
                     ERLATISANQALGSGFKIALRDLEIRGAGNLLGAEQSGHIAAVGFDAYARILQESMAE
                     MKGEPIVEEKELRIDLPVKAFIPPGWVAQEALRLELYRRISLAADHTVLEQIRAETVD
                     RYGALPEQVETLFAIASLRITARALGVEEISTFREQVRLKPVAIPEALQLDLEERVYK
                     AGFHPETQTLNLVPERVFGKDLAEFVERWLLEAATGDNVAPA"
BASE COUNT          359 a          830 c          798 g          405 t
ORIGIN      
        1 gaagagctgt gcttgcttgg ccagctgggc gtcggtgatg tcgttaccgg ccaccgaagc
       61 ggcgacacct tcggacgggg aaggttcggc gtcgctgcag gcagcgaaca ccacgacggc
      121 gacggcggac acggcgatga ggcggagcgt tcggggattc acgaacgctc agtctacgcg
      181 ggcaccgctt gcacgccccg ggccgtcaca gaaccgtcat gcgggggcga cgttgtcgcc
      241 tgtggccgct tcgagcagcc accgctccac gaattcggcc agatctttgc cgaacacccg
      301 ctcgggcacg aggttcaggg tttgggtctc gggatggaat ccggccttgt agacccgttc
      361 ttccaggtcg agctgcaggg cctcggggat ggcgacgggc ttgagccgta cctgttcgcg
      421 gaacgtcgag atctcctcga cgcccagcgc gcgcgccgtg atccgcagcg aggcgatcgc
      481 gaacaaggtc tcgacctgct cggggagcgc gccgtaccgg tcgacggtct cggcgcggat
      541 ctgctcgagc acggtgtgat cggccgccag cgagatccgg cggtagagct cgagccgcag
      601 cgcttcctgc gcaacccatc cgggcgggat gaacgccttc accggcaggt cgatgcgcag
      661 ctccttctcc tcgacgatcg gctcgccctt catctcagcc atcgactcct ggaggatgcg
      721 ggcgtacgcg tcgaagccga ccgcggcgat gtggccggac tgctcggcac cgagcaggtt
      781 gccggcaccg cggatctcca ggtcgcgcaa cgcgatcttg aagcccgagc ccagcgcctg
      841 gttcgcactg atcgtggcca atcgctcgcg ggcttcgtcg gtcatctccc gttgcggcgc
      901 gaagaagaag taggcgaacg cccgctcggt gctgcggccc acgcgacctc gcagctggta
      961 catctgcgac agccccagcg cgtcggcgcg gtcgacgatc aaggtgttcg cgttggggac
     1021 gtcgaggccg ctctcgatga tgacggtgct caccagcacg tcggcctccc ggttccagaa
     1081 ccgcatcatc tgctgctcga gcaggtcctc gtccatctgc ccgtgcgcgg tgacgacgcg
     1141 cgcgtcgggg agctgctcct ggagccacgc cgcgtgccgg tcgatcgtgg ccacccggtt
     1201 gtgcacccag aagacctggc cctcacgcag gagttcacgc cgcacggcac cgagcgacag
     1261 cccttcgtca tacgccccga cgtacgtgag cacaggctgg cggtcctcgg gcggggtgtc
     1321 gatcgtgctc atctcgcgga tgccggtgag cgccatctcc agcgtgcgtg ggatcggtgt
     1381 ggcggtcatc gtcagcacgt cgacgctggc acggaactgc ttcagccgct ccttgtgggc
     1441 cacgccgaac cgctgctcct catcgaccac cagcaggccc aggtccttga acgccatgtc
     1501 tttgcccagc agccgatgcg tgccgatcac gatgtcgacc ttgccgtcct tggcgtcctg
     1561 caccgtctcg gcctgctgag ctggactcac gaaccggctc agcatcctga cggtgaccgg
     1621 gaagggcgcg aagcgctcgc tgaaggtgat gaagtgctgc tcggccagca gcgtcgtcgg
     1681 caccagcacc gccacctgct tgccctccat caccgctttg aacgctgctc gcacagcgat
     1741 ctcggtcttg ccgaacccca cgtcgccgca gatcagccgg tccatgggct tggcaagctc
     1801 catgtcggcc ttcacctcgt cgatcgcggt gacctggtcc gaggtctcct cgtgggggaa
     1861 ggcatcctcc agttcctgct gccagggcgt atcggggccg aacgcgtgac cgggcaccga
     1921 catgcgcacc gtgtagaggc gcacgagttc gcccgccatg tcgcggacgg cgcgcttgac
     1981 cttggtggtg gcgcgtgccc agtcgctccc acccatgcgg tgcagtcggg gcgcgtcgcc
     2041 gccgatgtag cgcgccacca tgcccacctg gtccgagggc acgaacagct tgtcgccctg
     2101 cgcgtactcc aggatcaggt aatcgcgttc tgccccggcg agctcgcgat gggtgatgcc
     2161 cacgtagcgc cccacgccgt ggatgcggtg gacggcgaaa tcccccggtt ccagctcgtc
     2221 ggcgatcgac tcggtcttgc gccgggtgaa ccgcggagcg gtccgggtgt ggcggcgcga
     2281 accgaacagg tcctcctcgg tggccacggc cagcttcgcc gcttcgaaca cgaaccccga
     2341 ggccaggggc gattcgaccg cgtcgtcgac ggcgcccacc acctccttgg cg
//
LOCUS       DATDBZ010000476         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1603345, whole genome shotgun sequence.
ACCESSION   DATDBZ010000476 DATDBZ010000000
VERSION     DATDBZ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603345"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VIX62_11160"
     CDS             complement(<1..286)
                     /locus_tag="VIX62_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860836.1"
                     /translation="MPAFVRSLNRPVVAIIAVAALAGGIRLWGLSQPADLVFDEIYYA
                     KSGCVFIGGTDEICRVESSDEHYWVENKWDMGSWVHPPLGKWMTGMGIKAF"
     gene            complement(291..728)
                     /locus_tag="VIX62_11165"
     CDS             complement(291..728)
                     /locus_tag="VIX62_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEY5860837.1"
                     /translation="MTDGHKDYSGTPLWKKLGIKAGSRIVVERAPDGFDDVLTAIAPL
                     PPEVDLLARATKDIDVAVVFTTEFRVMERRFGPLAKALQPAGRLWIAWPKKASKVATD
                     LTFEVVQGFGLGRGMVDNKTASVTEIFQGCQFVYRLKDRPPIP"
     gene            793..1620
                     /gene="rsmI"
                     /locus_tag="VIX62_11170"
     CDS             793..1620
                     /gene="rsmI"
                     /locus_tag="VIX62_11170"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY5860838.1"
                     /translation="MQGTLWLVGTPIGNLSDLAPRARETFASVDLIACEDTRRTGRLL
                     AGLDIRKPLLSFFEGNERERVEGLVARMLAGASVALATDGGMPSVSDPGYRLVAACVE
                     SGIDVRVVPGPSAALAALVISGLPTDRFVFEGFLPRKAGERMRRLEALRDEQRTMVVF
                     ESPVRVRAMLDDALVALGDRRIAVARELTKLHEEVLRGRIAEVLAHLGETDPKGEVVV
                     VIEGRHEPEVPPMDALVDEARALVDTEAMRKRDAARLVAARHGAGANDVYRAMLEPD"
     gene            1642..>2254
                     /locus_tag="VIX62_11175"
     CDS             1642..>2254
                     /locus_tag="VIX62_11175"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEY5860839.1"
                     /translation="MDEAFRSEMAQGYAFDEPCLTLGGPMLDGEVDPDTDVVLPLSMV
                     NRHGLVAGATGTGKTKTLQILAGQLSDAGVPVFVADVKGDLTGLSQPGDAASSAVQVR
                     ASQLGLTFTPQAHPVELLSLSGALGAQVRATIHSFGPLLLAKVLDLNKTQTSVLSLVF
                     KYCDDQRFPLLDLADLRTTWKFLSSEEGQVVLEDDGGISKASVG"
BASE COUNT          344 a          752 c          777 g          381 t
ORIGIN      
        1 cgaatgcctt gatccccata ccggtcatcc acttgcccag gggtggatgc acccacgagc
       61 ccatgtccca cttgttctcc acccagtagt gctcgtcgct ggactcgacg cggcagatct
      121 cgtcggtgcc cccgatgaac acgcacccgc tcttcgcgta gtagatctcg tcgaacacca
      181 gatcggcggg ttgggacagg ccccacaacc ggatgccacc ggcgagcgcg gcgaccgcga
      241 tgatcgcgac gaccggtcgg ttcaggcttc tgacgaaggc gggcatcgcg tcagggtatc
      301 gggggccggt ccttcagccg gtagacgaac tggcacccct ggaagatctc ggtcaccgat
      361 gcggtcttgt tgtcgaccat gccccggccg agtccgaacc cctggaccac ctcgaaggtg
      421 agatccgtgg cgaccttcga agccttcttg ggccaggcga tccagaggcg ccccgccggc
      481 tgcagcgcct tcgcgagcgg cccgaaccgg cgctccatga cacggaactc cgtcgtgaag
      541 accaccgcga catcgatgtc cttggtggcg cgggcgagga ggtccacctc gggcggcagc
      601 ggtgcgatcg ccgtcagcac gtcgtcgaac ccgtcgggcg cgcgttccac gacgatgcgc
      661 gatccagctt tgatcccgag cttcttccac aacggggtgc ccgagtagtc cttgtgtcca
      721 tccgtcacga cggccgatcc tccgggtgat gcgtccgtgg tcgctcccgc gccatcgtgt
      781 caccctgggt ggatgcaggg caccctctgg ctcgtgggca cgccgatcgg caacctgagc
      841 gacctggcac ctcgagcccg cgagacgttc gcctcggtgg acctgatcgc ctgcgaagac
      901 acccggcgca ccggccggct gctggcgggc ctggacatcc gcaagccact gctgtcgttc
      961 ttcgaaggca acgaacgcga acgcgtcgag ggtttggtgg cccggatgct cgcgggtgcg
     1021 tcggtggcgc tcgccaccga tggggggatg ccgtcggtgt ccgaccccgg ctatcggttg
     1081 gtcgctgcct gcgtcgagtc ggggatcgac gtgcgggtgg tgccgggtcc ctccgcggcg
     1141 ctcgcggccc tggtgatctc ggggctaccg accgatcggt tcgtgttcga ggggttcctg
     1201 ccccgcaagg cgggggagcg gatgcgccgg ctcgaggcgc tccgcgatga gcagcggacg
     1261 atggtggtgt tcgaatcgcc cgttcgcgtg agggccatgc tggacgatgc cctcgtcgct
     1321 ttgggcgatc gcaggatcgc ggtggcacgc gagctcacca agctgcatga ggaggtcctg
     1381 cgtggccgca tcgcagaggt cctggcacac ctgggcgaga ccgaccccaa gggggaggtc
     1441 gtggtcgtga tcgaagggcg acacgaaccc gaggtgccgc cgatggatgc actcgtcgac
     1501 gaagcccgcg cgctcgtgga caccgaagcg atgcgcaaac gtgacgccgc acgattggtc
     1561 gcggcccgtc atggcgccgg tgcgaacgac gtctatcggg cgatgctcga gcccgactga
     1621 gtcggggtag gctcccgagc catggacgag gccttccgca gtgagatggc gcagggctat
     1681 gcgttcgacg aaccgtgcct cacgctcggc ggcccgatgc tcgacggcga ggtcgacccg
     1741 gacaccgacg tcgtgcttcc cctgtccatg gtgaaccgtc acggtctggt cgcgggcgcg
     1801 accggaaccg gcaagaccaa gacgctgcag atcttggccg gccaactgtc cgatgccggc
     1861 gtgccggtgt tcgtcgccga cgtcaagggc gacctgacgg gtctgtcgca accgggcgat
     1921 gccgccagct cggctgtgca ggtgcgagcc tcgcagctcg gcctcacgtt cacgccgcag
     1981 gcacaccccg tggagttgct ctcgctgagc ggggcgctcg gcgcacaggt tcgcgcgacg
     2041 atccattcgt tcgggccgct gctgttggcg aaggtgctcg atctcaacaa gacgcagacc
     2101 tcagtgctct cgctggtctt caagtactgc gacgaccagc ggttcccgct gctcgacctc
     2161 gccgacctcc gcacgacgtg gaagttcttg tcctcggagg agggccaggt cgtgttggag
     2221 gacgacgggg gcatctccaa agccagcgtc ggcg
//
LOCUS       DATDBZ010000477         3660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2393342, whole genome shotgun sequence.
ACCESSION   DATDBZ010000477 DATDBZ010000000
VERSION     DATDBZ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393342"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..512)
                     /locus_tag="VIX62_11180"
     CDS             complement(42..512)
                     /locus_tag="VIX62_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860840.1"
                     /translation="MSPFRRTVVPVVLFTLLAAACTGGGDEEPSAQPTSPSCAPGECV
                     VSSDPGDIAYQPGQYEYTFNGVTATFSLDGATGTLEVKNGSPAEIGKPGMYVISGDDQ
                     RYEGSVDAPAVIPEGGSATFTVTFPEQVDATSIGLMVLLFGGSNFGAMRPVPAA"
     gene            593..1060
                     /locus_tag="VIX62_11185"
     CDS             593..1060
                     /locus_tag="VIX62_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5860841.1"
                     /translation="MKQLELRRHARRDPSADRLAPEGRAQAEDVGRGSGRTYDLVFVS
                     PAERAAETAAWFLRGAGQQLPEHAVIGGLAGRDASGGSPEGMAAGVRALLDQLPDGGT
                     GLAISHTPLVERAAFGLTGVEVEPFAECEGILVSQDEAGTISIEELRVGTPAG"
     gene            1057..1707
                     /locus_tag="VIX62_11190"
     CDS             1057..1707
                     /locus_tag="VIX62_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY5860842.1"
                     /translation="MTAWSEHIVPAFALGAALGAAPGPVQLLILSETAKGGLVQGFRV
                     MLGANGMLFAVMVVLALGFSTTEPSQTVIDVLRVVGGLFLIYLAISELRTLRRHHLLG
                     EEAAIQASGPGARLGPTARGVMLVIVNPGAWIFFATTASAVVAEASADGGTDTALAAA
                     LAMTLGVSVTDFGSALIGTGSRALLGERGLTWVRTGLSVLLLAIGVAFVLQGFGLL"
     gene            complement(1715..2938)
                     /locus_tag="VIX62_11195"
     CDS             complement(1715..2938)
                     /locus_tag="VIX62_11195"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860843.1"
                     /translation="MDVEIRPITPDEFEAYLVALEVAFSARVEEGDLDRERSVAIPER
                     FLAAWDGDRIVGGAASLPKQMTLPGGRIATTAFVTAVGVQPTHRRRGINTALMRRQLD
                     DVHARDEAFAALWASEGSIYGRFGYGMASMFAEVKIDADRARFVRGYRPEGRVELLVR
                     DDAMPVMETVYDAVRRIRPGMLDLEGAWFGWRWWQRKSDKDAMAFYAIHRGERGEADG
                     YAAYVVKHEWPDSMPKLQVEIKELVATTPQGYADLWRFLFDIDLVGSVEGWNRPSDEP
                     LMWLMAEPRQLRMRLGDGLWVRLVDVPAALVLRGYRAPGRLVLDVQDRFCPWNEGRIE
                     LVVAEDGSATCGPTEDQPDVVCTVNDLGAVFLGGATFGQLHRAGQVAEGTDGAIERAD
                     VLFGTWPAPWCSFIL"
     gene            complement(2980..>3660)
                     /locus_tag="VIX62_11200"
     CDS             complement(2980..>3660)
                     /locus_tag="VIX62_11200"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY5860844.1"
                     /translation="VTSGESGQIAEGSDATPEILGEVREAEDRRSWEVLGRVPDRHEW
                     LRVPDHAVADHDTDELVERIATVLREEQPDVVMTFGPDGVTGHPDHIAVGEVATQAFH
                     RVREEGVPGFSRLLHQAIPQSMLDRWNEELVAKGQEPMDPTQMYQPRGVPDERVGVRV
                     DCTGVAMRKLAAVREHVTQLDGTQEHIGDEAQQLQALAFETHVVAWPATDPEAMVMTD
                     VFEGLDVG"
BASE COUNT          565 a         1301 c         1234 g          560 t
ORIGIN      
        1 gcgagtgtac gggagaggca acccgtgcac aacggaagat ctcaggcggc cgggacgggg
       61 cgcatcgcgc cgaagttgct cccgccgaac agcagcacca tcaagccgat cgaggtggca
      121 tcgacctgct cggggaacgt cacggtgaac gtggccgacc ccccttcggg gatcaccgcg
      181 ggtgcgtcca cggagccctc gtatcgctgg tcgtcgccgg agatcacgta catccccggc
      241 ttgccgatct cggcgggtga gccgttcttc acctcgaggg tgccggtggc cccgtccaac
      301 gagaaggtgg ccgtcacgcc gttgaacgtg tattcgtact ggccgggctg ataggcgatg
      361 tcgcccgggt cggaactcac cacgcactca ccgggggcac acgacggcga cgtcggctgg
      421 gccgagggct cttcatcgcc gcctccggtg cacgccgcag cgagcagggt gaagagcacg
      481 acgggcacga cggttcgacg gaaggggctc acggttgcgg catgatacgc gaaccgaatg
      541 cccggcgagc cgaacgtcag gtcgtcgtca caggaacagg aaacgaggaa ccatgaagca
      601 gctggaactc cgacgccacg ctcgccgaga cccctccgcc gaccgtctcg ctccggaagg
      661 ccgcgcccag gccgaggacg taggtcgcgg ctccggacgc acgtacgacc tcgtattcgt
      721 ctcccccgcc gaacgcgccg cggagacggc cgcctggttc cttcgcggcg ccggtcagca
      781 gttgcccgag cacgcggtga tcggcggcct cgccggtcgt gacgcctccg gcggcagccc
      841 tgagggcatg gcggcgggcg tgcgagccct gctggaccaa ctccccgatg gaggaaccgg
      901 cctggcgatc agccacacgc cgttggtgga gcgggcggcg ttcggcctca cgggtgtgga
      961 ggtggaaccc ttcgccgagt gcgaggggat cttggtgagc caggacgagg ccggcacgat
     1021 ctcgatcgag gaactccggg tcgggacgcc ggcgggatga cggcatggag cgagcacatc
     1081 gtgccggcgt tcgccttggg cgcggccctc ggcgccgccc ccggaccggt gcaactgctg
     1141 atcctgtccg aaacggccaa gggcgggctc gtgcagggct tccgggtgat gctgggagcc
     1201 aacgggatgc tgttcgcggt gatggtggtg ctggcgctcg ggttctcgac gaccgagcca
     1261 tcccagaccg tgatcgacgt gctgcgggtg gtcggtggcc tgttcctgat ctacctcgcc
     1321 atctcggagt tgcggacgct ccgacggcat cacctgctgg gtgaggaggc cgcgatccag
     1381 gcgagcggcc ccggcgcccg gctgggaccg accgcgcgtg gcgtgatgct ggtgatcgtc
     1441 aaccccggcg cgtggatctt cttcgccacc acggcgtccg cggtggtggc cgaagccagt
     1501 gccgacggcg ggacggacac cgctctggcc gccgccctgg cgatgacgct tggggtctcg
     1561 gtcaccgact tcggatcggc gctgatcggc acgggcagcc gggcgctgct gggcgaacga
     1621 ggactcacct gggttcgcac agggctgtcg gtcctgctcc tggcgatcgg tgtggcgttc
     1681 gtgctgcagg ggttcgggct gctgtgagcg tggcctagag gatgaacgag caccacggcg
     1741 ccggccatgt gccgaacagc acatcggctc gctcgatcgc cccatcggta ccctcggcca
     1801 cctgcccggc ccggtgcagc tgaccgaacg tagcaccgcc caggaacacg gcacccaggt
     1861 cgttgacggt gcagaccaca tcgggctggt cctccgtcgg cccgcaggtc gccgaaccgt
     1921 cctcagccac cacgagctcg atgcgccctt cgttccaggg gcagaatcgg tcctggacgt
     1981 ccagcaccag gcgcccggga gcgcggtagc cccgaaggac gagggcggcc gggacgtcca
     2041 ccagccgcac ccacaagccg tcgcccagtc tcatccggag ctgcctgggt tcggccatca
     2101 accacatcag cggttcgtcc gacgggcggt tccagccctc gaccgacccg accaggtcga
     2161 tgtcgaacag gaagcgccac agatcggcgt agccctgcgg cgtcgtcgcg accagttcct
     2221 tgatctccac ctgcaacttc ggcatggagt cgggccactc gtgcttcacc acgtaggcgg
     2281 cgtagccatc cgcctcgccg cgctcaccac gatggatcgc gtagaacgcc atcgcgtcct
     2341 tgtcagactt gcgctgccac caccgccacc cgaaccacgc gccttcgaga tcgagcatgc
     2401 cgggtcgaat gcgccgcacg gcgtcgtaca cggtctccat caccggcatc gcatcgtcgc
     2461 gaaccagcag ctcgacgcgc ccctcggggc ggtagccgcg cacgaacctc gctcggtccg
     2521 catcgatctt gacctccgcg aacatggacg ccatcccgta cccgaagcgg ccgtagatgc
     2581 tgccttcgga agcccagagc gccgcgaagg cctcgtcgcg ggcatggacg tcgtccagct
     2641 gacggcgcat cagcgcggtg ttgatccccc gccggcgatg ggtcggctgc acgcccacgg
     2701 ccgtgacgaa ggcggtggtc gcgatccggc cgcccggcag ggtcatctgt ttgggcagcg
     2761 aggcggcacc acccacgatc cggtcgccgt cccacgccgc caggaaccgt tcggggatcg
     2821 ccacggatcg ttcccggtcg agatcgccct cttcgacacg cgcactgaag gccacctcga
     2881 gcgccacgag gtaggcctcg aactcatcgg gcgtgatcgg tcggatctcg acgtccatgc
     2941 cgcccagcct accgccgggt ggcgtcgcct ccggtcgggt cagccgacgt cgagaccctc
     3001 gaacacgtcg gtcatcacca tcgcttccgg atcggtggcg ggccacgcga ccacgtgggt
     3061 ctcgaacgcg agcgcctgca gttgttgggc ctcgtccccg atgtgctcct gcgtgccgtc
     3121 caactgcgtg acgtgttcgc gcacggcggc gagcttgcgc atcgcgacgc cggtgcagtc
     3181 cacccgcacc cccacccgct cgtccggcac gccacggggc tgatacatct gcgtcggatc
     3241 catcggctcc tggcccttgg ccacgagctc ctcgttccag cggtcgagca tcgactgcgg
     3301 gatcgcctga tgcagcaagc gggagaatcc gggaacgccc tcttcgcgca cacgatggaa
     3361 cgcctgcgtc gccacctccc ccaccgcgat gtggtccggg tggccggtga cgccatcggg
     3421 cccgaaggtc atcaccacat cgggctgctc ctcgcgaagc acggtggcga tgcgctcgac
     3481 gagctcgtcg gtgtcatggt cggccaccgc atgatcgggg acccgcagcc actcgtgccg
     3541 gtccggcacg cgacccagca cctcccacga acgtcgatct tctgcctcgc gaacctcacc
     3601 caagatctcc ggcgtggcgt ccgagccttc ggcgatctga ccgctctcgc cgctggttac
//
LOCUS       DATDBZ010000478         2218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2393360, whole genome shotgun sequence.
ACCESSION   DATDBZ010000478 DATDBZ010000000
VERSION     DATDBZ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393360"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1097)
                     /gene="purM"
                     /locus_tag="VIX62_11205"
     CDS             complement(102..1097)
                     /gene="purM"
                     /locus_tag="VIX62_11205"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012360852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY5860845.1"
                     /translation="MTSYRDAGVDIEAAAKAVTLIGDLAARARRPEVADEVGGFAGLF
                     RIGQGRMLAAATDGVGTKLEIARMTGRLDTVGIDLVAMCADDVVCTGAEPLFFLDYLA
                     VGKVVPERVASLVEGVAEGCRRAGCALLGGETAEHPGVMPDDQFDMAGFCVGSVAEAD
                     LLGPDRVREGDVLIGLASTGLHANGYSLVRSALLGRYDLAETPEGLSRPLADELLEPC
                     AIDAPAVLALARDGLVHAAAHITGGGFFENIPRALPPGLGAQVHRGSWSEPAIFSLVQ
                     MASGTTDDEMFATFNMGIGMVLVVDRDHEDEVLQRHEGRAFSMGAVTTGSGVRFD"
     gene            complement(1094..>2218)
                     /gene="purF"
                     /locus_tag="VIX62_11210"
     CDS             complement(1094..>2218)
                     /gene="purF"
                     /locus_tag="VIX62_11210"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY5860846.1"
                     /translation="FKNDGAHTLALGHNGNLVNTHELAVRVGKAGTATTDSDLVATLL
                     ADQMTVTGGLLEAVMQVLPTLQGAYCFVMMDERSVYAARDPYGVRPLSIGRLPNGFVV
                     ASETCALDIVGAAFVRDVEPGELIRFDDRGMHSVRFSESPRRALCIFESVYLARPDSR
                     LGGVSVHESRREMGRILAREHPVDADMVIAVPTTGHSAAQGYSEVSGIPYGDGLYKNQ
                     YVGRTFIQPSQTLRDRGVKLKLNPLPDSIRGKRLVVVDDSIVRGTTTKQIVAALREAG
                     ATEVHIRITCPPVKWPCFYGIDMPTRQELIGADLTVEEIRAYVAAESLGYLSLEGMVE
                     AAGDEKDRFCRACFDGEYPIPIPAEAGKFVLEDQLRLPTS"
BASE COUNT          388 a          764 c          722 g          344 t
ORIGIN      
        1 atcggccgcg cgggtatatc tggatgccgc cttcggacgg gggcagacca ccgttggtcg
       61 ggggtaccgg gggacatcga cctagtcagg ggtagccatg ttcagtcgaa ccgaacaccg
      121 gatcccgtcg tcaccgcgcc catcgagaac gctcggccct cgtggcgctg caggacctcg
      181 tcctcgtgat cgcgatcaac caccagcacc atgccgatgc ccatattgaa cgtcgcgaac
      241 atctcgtcgt ccgtggtccc cgacgccatc tgtacgagcg agaagatcgc cggttcggac
      301 caggaacccc ggtgcacctg ggcgccaagt ccgggcggca gtgctcgagg gatgttctcg
      361 aagaaccctc cgccggtgat gtgagccgca gcgtggacca ggccgtcccg cgccagcgcg
      421 aggaccgctg gcgcatcgat cgcgcatggc tcgagcaact cgtctgcgag cgggcgcgac
      481 aacccttccg gcgtctccgc cagatcgtag cgacccagca acgccgagcg caccagcgag
      541 taaccgttgg cgtgcagacc cgttgacgcg aggccgatca acacgtcgcc ttcccgtacc
      601 cggtcaggac ccagcaggtc ggcctcggca acggaaccca cgcagaaccc cgccatgtcg
      661 aactggtcat cgggcatcac accggggtgc tcggccgtct cccctcccag cagggcacat
      721 ccggctcgac ggcagccttc ggcgacaccc tccacgaggg aagcgacccg ttcgggcacc
      781 accttgccca cggccaggta gtccaagaag aagaggggct cggccccggt acacacgaca
      841 tcgtcggcgc acatcgccac caggtcgatg cccacggtgt cgagccgacc cgtcatgcgg
      901 gcgatctcga gcttggtccc caccccgtcg gtcgcggcgg ccagcatgcg accctgaccg
      961 atccggaaca gtcccgcgaa cccacccacc tcgtcggcga cctcgggacg gcgcgcccgt
     1021 gctgcgaggt cgccgatcaa cgtcacggct ttggcggcag cctcgatatc cacaccggcg
     1081 tcgcggtagg aggtcacgac gtggggagcc ggagctggtc ctcgagcacg aacttgccgg
     1141 cctcggctgg gatggggatc gggtactcgc catcgaagca cgcgcgacag aatcggtcct
     1201 tctcgtcgcc tgctgcttcg accatgccct cgagcgagag gtagcccagg gactcggcag
     1261 ccacgtaggc gcggatctcc tcgaccgtca ggtcggcccc gatcaactcc tgtcgagtgg
     1321 gcatgtcgat gccatagaag cacggccatt tcaccggcgg gcaggtgata cggatatgga
     1381 cctcggtcgc gccggcctca cgcaacgccg ccacgatctg cttcgtggtg gtgccgcgca
     1441 cgatggaatc gtcgaccacc accaagcgct tgccacggat cgagtcgggc aacgggttga
     1501 gtttgagctt caccccgcga tcacgcaggg tctgggacgg ctggatgaac gtgcgcccga
     1561 cgtactggtt cttgtagagg ccgtcgccgt aggggatccc gctgacctcg ctgtagccct
     1621 gcgccgcact gtgtcccgtg gtgggcaccg cgatcaccat gtctgcgtcc accgggtgtt
     1681 cgcgcgcgag gatccggccc atctccctgc gagattcgtg cacggagaca ccgccgagtc
     1741 gtgaatcggg tcgcgccagg tagaccgact cgaagatgca cagcgcccgg cggggactct
     1801 cggagaaccg gaccgaatgc atgccgcgat cgtcgaagcg gatcaactcg ccgggctcga
     1861 catcacgcac gaaggcggcg cccacgatat cgagggcgca ggtctcggag gccacgacga
     1921 acccgttggg gaggcgcccg atcgacagcg gtcgcacgcc gtatgggtcg cgggccgcgt
     1981 agacgctgcg ctcatccatc atcacgaagc agtacgcacc ttgcagggtg gggagcacct
     2041 gcatgactgc ttccaggagg ccaccggtca ccgtcatctg gtcggcaagc agcgtggcga
     2101 ccaggtcgga atcggtcgtg gcggtgcccg ccttgcccac ccgcacggcg agctcatgcg
     2161 tgttgacgag gttgccgttg tgacccaggg ccagggtatg tgcaccgtcg ttcttgaa
//
LOCUS       DATDBZ010000479         2787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1804672, whole genome shotgun sequence.
ACCESSION   DATDBZ010000479 DATDBZ010000000
VERSION     DATDBZ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804672"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..954)
                     /locus_tag="VIX62_11215"
     CDS             complement(142..954)
                     /locus_tag="VIX62_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5860847.1"
                     /translation="MVQPDLTDDLVFAHELADAAAAVTLAWFGDRVPVELKDDATPVT
                     EVDRAAEQAIRAAIEVRFPLDGVLGEEQGLVSGSSGRRWIVDPIDGTKLFAEGIPLWT
                     TLIALEVDSRLVLGIADAPAIGDRYVGVSGQGAWRGSQRLRVSDVARLDDAFVAHSGL
                     EEWITGGHEESLLAVAARARRTRGLSDAWGHLLVAQGSVEAVLEHEPCQAWDWSATQV
                     IVHEAGGRLSTLDGSPPTAGSDLLVSNGVVHEEIVTALVSVGRTFVSDGGTR"
     gene            complement(936..1895)
                     /locus_tag="VIX62_11220"
     CDS             complement(936..1895)
                     /locus_tag="VIX62_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860848.1"
                     /translation="MTDGNPFDVDGVWLKSAFHTHTKRSDGELDPSAHVLHHEWAGFD
                     VCAITDHWTLTHEPSTDRILVITGAELAADPYGDAPNDSEILAIGISEIPEDPGGDRN
                     RWGPIDAYHYKTFPDLATAATCIADQGGVSFVAHPYWSGLSLETLLAAGTDGLELFNS
                     SAHRENLRGDSSYVWDQCLDRNERLWAFGTDDCHYPGFDIGDAWTMVRAAERTEEAVL
                     DALRHGHHYASSGPELRGIAVDGDALEVRCSPARAVWMVSRYETGWAVHAGDRNRMEE
                     ARVLERDDRDLIVRARFSPSMELPYRRIVIEDERGRRAWSNPI"
     gene            2026..>2787
                     /locus_tag="VIX62_11225"
     CDS             2026..>2787
                     /locus_tag="VIX62_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY5860849.1"
                     /translation="MRATDGADRVVRVLDALATAPEGLGVSTIARTLQIHRSTASRLL
                     ATLASGGLIERDVQTRAYRLGSRMVGLAAVAVARLPVVSQARPELEALSTMTSETANL
                     AILDGDHVVYVDQVTPAHTVVMASWVGRRSPVHASSSGKVLLAWGDPKAREALLGRRL
                     EPITDRTMTDPRALRRLFDEVRRRGFVASNGELESGLITIAAPVLIDRLAVAAVSISG
                     PTYRIPTRDVARLGRAVRDAAGAVGHRMAGRTITAD"
BASE COUNT          469 a          998 c          874 g          446 t
ORIGIN      
        1 tgagctcgat gtcggtgccg acacgcaaga ccaactcctg ttcgtccgca ccgggcgttt
       61 ccgaggactg cgctgcggcc agcgtcgtgg tccccaggac cacggccatg atgacgacgg
      121 acaatcggat caccctgtgc ttcatcgagt tcctccatcc gatacgaacg ttcgcccgac
      181 cgaaaccagc gccgtcacga tctcttcatg caccacaccg ttcgatacga gtagatcact
      241 gcccgcggtc ggcggcgacc cgtccagggt gctcaatcga ccacccgctt cgtgcacgat
      301 gacttgggtt gccgaccagt cccatgcttg acacggctcg tgctcgagca ccgcctccac
      361 cgacccctgt gcaaccaaca ggtggcccca cgcatcactc aacccccggg tgcggcgggc
      421 acgggcggcg accgcgagca aggactcctc gtggcctccc gtgatccact cctccaggcc
      481 ggaatgtgcc acgaacgcgt catcgagccg cgcgacgtcg gacacccgca ggcgctgcga
      541 gcctcgccag gctccctggc ccgacacccc gacgtatcga tcgccgatcg ctggggcatc
      601 ggcgatgccg agcaccaggc ggctgtccac ctccaacgcg atcagcgtgg tccacagcgg
      661 gatgccttcg gcgaagagct tcgtaccgtc gatgggatcc acgatccagc gtcgcccgct
      721 ggatccggac accagtccct gctcttcacc cagcacccca tcgaggggga accgcacctc
      781 gatcgcagct cggatcgcct gctcggcggc ccgatcgacc tcggtgacgg gcgtggcgtc
      841 gtccttcaac tccaccggca cacggtcgcc gaaccacgcc agggtcaccg cggcggcggc
      901 gtccgccaac tcgtgtgcga agacaagatc atcggtcaga tcgggttgga ccatgccctc
      961 cgccctcgtt cgtcctcgat cacgatccgc cgatacggca gttccatcga cgggctgaac
     1021 cgggcccgaa cgatgaggtc gcgatcgtcc ctctcgagca cgcgtgcctc ttccatccgg
     1081 ttgcggtccc ccgcatgcac cgcccagccc gtctcgtatc gagacaccat ccacactgcc
     1141 cgagcggggg aacaccgcac ctcgagcgca tcaccgtcca cagcgatgcc gcggagttcc
     1201 ggacccgacg acgcgtagtg atggccgtga cgcaaggcgt ccaggaccgc ctcctcggtg
     1261 cgctcggcag cccgcaccat ggtccacgca tcaccgatat cgaagcccgg gtagtgacag
     1321 tcatcggtac cgaacgccca gagtctctcg ttgcggtcaa ggcattggtc ccacacgtag
     1381 gaggagtccc cgcgcaagtt ctcccggtgc gcgctggagt tgaagagctc cagaccatcc
     1441 gttccagccg ccaacagcgt ctcgagcgag aggccactcc aataggggtg cgccacgaac
     1501 gagacaccgc cttgatcggc gatgcaggtg gcagccgtgg ccagatccgg gaacgtcttg
     1561 tagtggtagg cgtcgatcgg accccaccgg ttccggtcac cgcccgggtc ctcggggatc
     1621 tcgctgatac cgatcgcgag gatctcgctg tcgttggggg cgtcgccgta gggatcagcg
     1681 gccagttcgg cgcccgtgat caccaggatg cggtcggtgc tgggctcgtg cgtcaacgtc
     1741 cagtggtcgg tgatcgcgca cacatcgaag cctgcccact cgtgatggag gacatgcgcc
     1801 gagggatcga gttcaccgtc cgagcgcttc gtgtgggtgt ggaacgcgct cttcaaccac
     1861 acgccgtcga catcgaacgg gttcccatcg gtcacgatcc gaccctcctt cgttgactcg
     1921 cttcgctgca ggcttcgctg ctgggctcac gccgacggta cccagaggcc gcgaagcggc
     1981 acaagcgaat gcgtgccctc tcggcaacac gagtagtctc gtgggatgcg cgccaccgac
     2041 ggagccgacc gggtcgtgcg ggtcctggat gcactggcca cggcacccga aggcctcggt
     2101 gtctcgacaa tcgcccgaac gctccagatc catcgctcga cggcgtcgcg cttgctggcc
     2161 accctggcga gtggcggcct catcgaacgc gacgtccaga cccgcgccta ccggctcggt
     2221 tcgcggatgg tgggcctggc cgccgtcgcc gtcgcccggt tgccggtcgt gagccaagcc
     2281 cgtccggagt tagaggcgct ctccacgatg acctcggaaa cagccaacct cgcgatcctc
     2341 gacggcgacc atgtggtgta cgtcgatcag gtgacgccgg cccacaccgt ggtcatggcg
     2401 agttgggtcg gccgtcgcag tccagtgcac gcgagttcca gcgggaaggt gctgttggcc
     2461 tggggcgacc cgaaggcgag ggaggcgctc ttgggccggc gtctcgaacc catcacggat
     2521 cgaacgatga ccgacccacg agccttgcgc cggctcttcg acgaggtccg ccgtcgaggc
     2581 ttcgtcgcga gcaacggaga actggagagc gggctcatca cgatcgcggc cccggtcctg
     2641 atcgacagac ttgccgtcgc cgcggtcagc atctccggac ccacctaccg gataccgacg
     2701 cgggacgtgg cgcgcctggg gcgcgccgtc cgtgacgcgg cgggcgccgt cggccaccgc
     2761 atggccggac gaacgatcac cgcagac
//
LOCUS       DATDBZ010000480         2908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1804727, whole genome shotgun sequence.
ACCESSION   DATDBZ010000480 DATDBZ010000000
VERSION     DATDBZ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804727"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VIX62_11230"
     CDS             complement(<1..671)
                     /locus_tag="VIX62_11230"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5860850.1"
                     /translation="MTDRRLYLIRHARPDYTSTAHTETPLGRQYDPPLGEVGMSQAQR
                     LAERLVLLPRPAAIYSSTLARARETAQVYADRVAMPVVERDELCEWFGGEWEAKDFEQ
                     IFEEHPEAIDQFRNQNPAWHLAPGSERAEDFQQRCVAAVEAIITAEREGDVVVVAHGG
                     VINAYFSHILGIRDQDMFFLPENTSLNTVVIRGEERLAWFLSDASHLTDPGWFELREW
                     ERDLA"
     gene            complement(668..1054)
                     /locus_tag="VIX62_11235"
     CDS             complement(668..1054)
                     /locus_tag="VIX62_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860851.1"
                     /translation="MSDVADEARARMWQTGGRFVAQVLDVPAYIEVTGISKRECVAEL
                     RRITGDDVALTIELVPDVVGVAEAAEIMGWDKRRVVTYIDRGSFPEPITALASGRIWL
                     REDVQAYADTWQARRAAKTAVPEASR"
     gene            complement(1051..1206)
                     /locus_tag="VIX62_11240"
     CDS             complement(1051..1206)
                     /locus_tag="VIX62_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860852.1"
                     /translation="MKPSTANDPAACIPVNESKRLPCDLCGHPMEPEHAHYRCPACHY
                     ILPCCGW"
     gene            complement(1233..1532)
                     /locus_tag="VIX62_11245"
     CDS             complement(1233..1532)
                     /locus_tag="VIX62_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860853.1"
                     /translation="MPRKNRRNPEYFRAPEPVDTARTTPDAPGWAQAPGYEVRAVGGQ
                     KAYRCPGCDHEVRAGIAHLVVVPVGDVDARRHWHTECWRRELRRQGLHRPSATAD"
     gene            complement(1532..2194)
                     /locus_tag="VIX62_11250"
     CDS             complement(1532..2194)
                     /locus_tag="VIX62_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY5860854.1"
                     /translation="MPEERFTIDVPEKGTVSAAYVRPRDAFASLALFHGAGAGMDHPF
                     MAGFARAIADEGVATLRCNFPYMEAGKRSPDREPIAVATVRAAFAAATERGGGGEPVW
                     VGGKSFGGRMASVAVAEGMPAAGLVFLGYPLHPPGKPERVRDEHLYGIEVPMLFLQGM
                     KDPFATAAVLEPVLVKLPTATLHAVDGGGHSLERSRSDDAREVAASFAPLVAGFMRGG
                     RS"
     gene            2220..>2908
                     /locus_tag="VIX62_11255"
     CDS             2220..>2908
                     /locus_tag="VIX62_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY5860855.1"
                     /translation="MTAKGTLHLGTSGWSFDGWKHGVFYPEGLKNREMLHYYTTQLSS
                     VEINYTFRRFPTEKSLTTWREQAAEGFVFTLKANQRITHFKRLRDTDDDVRDFLERAK
                     LLGDRLGCVLFQCPPSLQYDRELIERFVGYLPPGPPAFAMEFRHASWNEAKDLLRDQG
                     IAWCVAETDERDPTPEELSWEPVGYLRLRKTEYGDDELEEWAGRIRPALESGADIFCY
                     FKHEDLGASPQ"
BASE COUNT          442 a         1001 c         1022 g          443 t
ORIGIN      
        1 tcggcgaggt cgcgctccca ctcgcggagc tcgaaccagc cggggtcggt gaggtgcgag
       61 gcgtcggaca ggaaccaggc gagccgctcc tcgcctcgga tcacgaccgt gttcaggctc
      121 gtgttctcgg gcaggaagaa catgtcctgg tcgcggatgc ccaggatgtg gctgaagtac
      181 gcgttgatca cacccccgtg cgcgaccacc acgacatcgc cttcgcgttc ggcggtgatg
      241 atcgcctcga ccgcggcgac gcaccgctgc tggaagtcct cggcccgctc actgcctggc
      301 gcgaggtgcc aagccgggtt ctggttgcgg aactggtcga tcgcctcggg gtgctcttcg
      361 aagatctgct cgaagtcctt ggcctcccac tccccgccga accactcgca gagctcgtcg
      421 cgctccacca cgggcatcgc gacgcgatcg gcgtagacct gtgcggtctc tcgggcacga
      481 gccagcgtcg aggagtagat cgccgcgggg cggggcagca acacgagccg ctctgcgagg
      541 cgctgggcct gcgacatccc cacctcgccg agcggcgggt cgtactggcg gcctaatggc
      601 gtctcggtgt gggcggtcga ggtgtagtcg ggtcgcgcgt gacggatcag gtacaagcgg
      661 cggtcggtca ccgggacgcc tcaggcacgg ccgtcttcgc ggcgcggcgc gcctgccacg
      721 tgtcggcgta ggcctgcacg tcttctcgga gccagatgcg gcccgacgcg agcgcggtga
      781 tcggctcggg gaaggacccc cggtcgatgt aggtcacgac ccgtcgcttg tcccacccca
      841 tgatctcggc ggcctcggcg acaccgacga cgtcgggcac gagctcgatc gtgagcgcga
      901 cgtcgtcgcc cgtgatccgg cggagctcgg cgacgcactc gcgcttgctg atgccggtga
      961 cctcgatgta ggcgggcacg tcgagcacct gggccacgaa gcggccaccg gtctgccaca
     1021 tgcgagcgcg cgcctcgtcg gccacgtcgc tcaccagccg caacagggaa ggatgtagtg
     1081 gcaggcgggg caccggtagt gcgcgtgctc gggttccatc gggtgcccgc acaggtcgca
     1141 cgggaggcgc ttggactcgt tcaccgggat gcaggcggcg ggatcgttgg ccgtcgacgg
     1201 tttcatcggg ccatcgtagt ccgaggcgcc ggctagtccg cggtagccga cggccgatgg
     1261 aggccctggc ggcggagctc cctgcgccag cactccgtgt gccagtgacg gcgggcgtcg
     1321 acgtcgccga ccggcaccac gaccaggtgg gcgatgccgg cccggacctc gtgatcgcac
     1381 cctgggcatc gatacgcctt ctgaccgccg accgcgcgca cctcgtatcc gggggcctgt
     1441 gcccagcccg gtgcgtcggg cgtcgtgcgg gcggtgtcca ccggctccgg ggcacggaag
     1501 tactcagggt tgcggcggtt cttcctcggc atcaggagcg ccccccacgc atgaaccccg
     1561 ccacgagggg ggcgaacgag gcggcgacct cgcgagcgtc gtcggaacgc gaccgttcca
     1621 gtgagtgccc acccccgtcg accgcatgca gcgtcgcggt cgggagcttg accagcacgg
     1681 gttcgagcac ggccgcggtg gcgaaggggt ccttcatgcc ctgcaggaac agcatcggca
     1741 cctcgatgcc gtacagatgc tcgtcccgca cccgctccgg cttgcccggg ggatgcagcg
     1801 gatacccgag gaagacgagt ccggcggctg gcatgccctc ggcgaccgcg accgacgcca
     1861 tacgccctcc gaacgacttc cccccgaccc agacgggctc accgccgccg ccccgctcgg
     1921 tggccgccgc gaaggccgcc cgcaccgtgg cgacagcgat cggttcacgg tccggtgaac
     1981 gcttgccggc ttccatgtag gggaagttgc atcgcaacgt cgccacgccc tcgtcggcga
     2041 tcgcccgggc gaagccggcc atgaacgggt ggtccatgcc cgcgccggcg ccgtggaaca
     2101 gcgcgagcga ggcgaacgca tcgcggggcc gcacgtacgc ggcgctcacc gtgcccttct
     2161 cgggcacgtc gatcgtgaac cgttcctcgg gcatcggcac gcgtgccatc atgcatgcca
     2221 tgacggcgaa gggcacgttg cacctcggca cgtccgggtg gtcgttcgac ggctggaagc
     2281 acggcgtctt ctacccggag ggcctgaaga accgagagat gctgcactac tacacgaccc
     2341 agttgagctc ggtcgagatc aattacacgt tccgccggtt cccgacggag aagtcgctca
     2401 cgacctggcg cgagcaggcc gccgagggct tcgtgttcac cctcaaggcc aaccagcgca
     2461 tcacgcactt caagcgcttg cgcgataccg acgacgacgt gcgggacttc ctcgagaggg
     2521 cgaagctgct cggcgatcgg ctcgggtgcg tcctgttcca atgcccgccc agcctgcagt
     2581 acgacaggga gctgatcgag cggttcgtgg gctacctgcc gccgggccct ccggcgttcg
     2641 cgatggagtt ccgtcacgcg tcgtggaacg aggcgaagga cctgctgcgc gatcagggca
     2701 tcgcgtggtg cgtcgcggag accgacgagc gcgatcccac acccgaggag ctctcgtggg
     2761 agccggtggg atacctgcgg cttcgcaaga ccgagtacgg cgacgacgag ctcgaggagt
     2821 gggcgggtcg gatccgcccg gcgctcgagt ccggggccga catcttctgt tacttcaagc
     2881 atgaagatct cggggcctca ccgcagat
//
LOCUS       DATDBZ010000481         9046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2208522, whole genome shotgun sequence.
ACCESSION   DATDBZ010000481 DATDBZ010000000
VERSION     DATDBZ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2208522"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VIX62_11260"
     CDS             complement(<1..915)
                     /locus_tag="VIX62_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5860856.1"
                     /translation="MADDPREPFDPQADAIAGRRVADLLEHIPATVFILTNEVPPRLL
                     YVNGQVERLTGYFRQAWLEDPDLWMRSMHPADQERVMDRWAESVRAASSFLCEYRIVR
                     PDGTVASVREATDPVRNLDGGVVCWQGVTHDITDRQAAEEALTRSEARYRALVERLPA
                     VVYVDSDEVDPLSLYVSPNSEEVLGYAPSRYLEDPGLWYATIHPDDLTNVKQAWAGVI
                     ESRDPFTLEYRMVRPDGGSLWVRDSSIPIMDEVGDTLFWQGVMLDITAQKLAEEDLRR
                     SETRYRVLVEQVPATVYICTDEERPRTLF"
     gene            complement(963..1253)
                     /locus_tag="VIX62_11265"
     CDS             complement(963..1253)
                     /locus_tag="VIX62_11265"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008418097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY5860857.1"
                     /translation="MKECKNLDEVREGIDAIDRQIVGLLSERSGYIEQAARIKNSAAE
                     IEVPERVEEVVSNVRGLATEYGMSPDIAEDVYRVMVARFIEFEMQIYRETLP"
     gene            1383..1471
                     /locus_tag="VIX62_11270"
     tRNA            1383..1471
                     /locus_tag="VIX62_11270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1417..1419,aa:Ser,seq:tga)
     gene            complement(1515..2525)
                     /locus_tag="VIX62_11275"
     CDS             complement(1515..2525)
                     /locus_tag="VIX62_11275"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5860858.1"
                     /translation="MDALEAALDAAAERTAFSGVVRVDRGGATELEKAYGLADRAHGI
                     PNTVDTRFMLASVAKGFTALAVMSLVADGTLQLSTTARSVLGDDLPLIADDVTVEHLL
                     AHRSGIGDYLDEDERDDDINSYTSPVPVHRLDTTERFLEVLDGFPTVSRADERFSYNN
                     AGYVVLALIAERASGVGYHELVRERVTEPAGLAETEFWRTDELPGDHAIAYLHAAGQR
                     SNALHLPVLGTGDGGISSTAADLAAFWRALYGGRIVPAELVAEMTRPRSEWPEEDRRY
                     GLGFHLHRTNEAVWLEGYDAGVSVCTTHDPVSGITHSAIANWSDGAWPMLSVLDEHLG
                     TA"
     gene            complement(2580..3989)
                     /locus_tag="VIX62_11280"
     CDS             complement(2580..3989)
                     /locus_tag="VIX62_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5860859.1"
                     /translation="MPNDAVDIARCTDLVRTYRTPTGEVQALRGVSATFTRGAVTAVV
                     GPSGSGKSSLLRLMAGLDRASAGTLEVDGTIVTSASARARRHLRRSKVGYVFQRPSDN
                     FVPYLSVGAHLRMAAGRSPAGADLAELLDALGIGHRAENLPGELSGGEQQRAAFGQAL
                     ATGSPLVVADEPTAELDGPSGAAVLERIRELAERGISFVIATHDPAVVAVADHTLTLD
                     HGSVRGSVTDAGGEWEDRQLDELRWPTSMEPLWDDGPVVDLVGVSKTYGHGEEAVRAL
                     VDVDLEARVGQVIALVGRSGSGKTTLLHSIAGWEIPDQGVVSAPGGREPDWSEIAVLP
                     QRLGLMEELTVQENVEYPARVAGDLADRRELVASLLRRFGLEELRDRAPRETSLGEQQ
                     RTALARVLVMRPQVLIADEPTGHQDAAWVSKIFATLQEAASLGTCCIVATHDGTLERF
                     ADRTLSMADGRIVTTSVGS"
     gene            complement(3979..6756)
                     /locus_tag="VIX62_11285"
     CDS             complement(3979..6756)
                     /locus_tag="VIX62_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860860.1"
                     /translation="MARTDTRTPTIPSGRPIWRRAPTALRHSPALLAALALSAFLVSI
                     TAASYPLFLSASKSQLLAEQIADPLVTNYGTGITYRATRVNFTTRAPGGGSLIDERAA
                     AFAERTAESPELEPAQEQILGTTVDVTLPGGRTPPSGGVVGRLFAGTNVLDNVTILAG
                     ADGPGVWLPDLLARGARLRPGDEFELTVDGHHETVTLDGIYRALYTQPRSGYWRTWAD
                     DIYAICPGYECPLPPQPILVDRSQLLDLGTRLGDPEGTFALVAPASADPPLTLDEARD
                     LAGFADRFEADMSTKDTELFRLFRCCGDVFIQSNCVQFFCPASDVSYTSLAGQVVGQV
                     DERLISVQGPLLVLLIAGLGISLAIVAAAGVFVLMSRRVEVGVLSVRGWGGSAVGAKA
                     VLEAVLPFLAGGIVGFALASVTIAAVGPPGRIESSARSTAVTAAVIAVAASLLVVGLV
                     SAAVFLSSHDHRHRLTRVLGFVPWELAAFAAAWVMARRLEGGGAIVESGGIERPGAAV
                     FLFPLLLALAIGILVARVMTIALTIGHRRASGSQGAGPWWLASRRLRTLGRLPQLLLI
                     AGSLTLAVFVSSQGMVHSLRTTVDAKAKVFVGSDVQVVVSRDIEPAPGFGFPVTRVVR
                     SRDAGAFTGSTLRYDLLAIDPDTFEAAAFWNDALSDDSLADLVTRLPSTGGAALPVIL
                     SNGGDYDPSSIDLSDKTVPIEMVGRATSFPGTSTSDRPLLVVDRDALAEHFTDIPDPL
                     NTPMASAEFWVAGPTDEILASLADIGATPYSVLTADEVEDIPFIDAAIQTFLMLDILG
                     IVAMVLLVVVAMIYLQARQRSRVVATELSGRMGQADGTMRRSLVLELGTVLIGALVVG
                     AVVGAVATRLVAPRLDPLPTIPPDSLIVLPVVGIAIGLLATAIASVAGALLADRSARR
                     VPLGEVLRVAE"
     gene            6889..>9046
                     /locus_tag="VIX62_11290"
     CDS             6889..>9046
                     /locus_tag="VIX62_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY5860861.1"
                     /translation="MSEGYHLSRRMFVRLSAGAVAATALPIPKALGSERGGTCDPFST
                     PPSFQGVVPSPEDVLGFPIGVDRETTAGEINTYLDAVGGASNRVRVRTLGDSVRGRPI
                     RYALVGDPANVTNQGLAQIQADTATIRDPATPQTTVDALAGTTPVILWIAGNLHGGEE
                     SGADASLQLLYELADRDDCGVTDILARALIVIIPTQNPDGREIGFRRNGFAFDLNQDH
                     LVRTQVETEGKLALMKQYPPAVLTDHHEFGYYRSFFPPNDDPVYHETPENTVRQIYDL
                     FGPAFAREFRRNDWQFFNEGYGYDLFAPRFTDTIAVMAYAAAGMTIEVYDGAPLDRRF
                     ARHLTVMWSMIGTGAANRKRLQRNLHESYVQALDEGRRGELEPNRTYEPHSRPVRRVP
                     DQKVRHYFFKDEPAKRREIAMLIHRLQEMDVAVYQLNAPLRVPDLKPYLESPHAGTVP
                     AGTYWIPMAQPQKRFIQAALNEDPYNPTNQAYRVSSWSLPLAYNLTGWLSGARLDPDA
                     DPVAPVGEPGPPTLPQPLPAVRIMHLSREFTAYEEIGHTRWLFRRSWELPFTEMDADE
                     VAAGIDGVDVLVVPAGGINEGLRRLGKAGQRELIRWVNHGGRLVAWRYGAARLAYTLG
                     ISKAVYDMVPASIDGPMLKANIDADSPLGDGVGRHAWPLVDTRAMHAPDAVSPITFPT
                     QASGDFRVSGVRRGTRFLMGSTAVADEPVGRGRSIVF"
BASE COUNT         1474 a         3089 c         3149 g         1334 t
ORIGIN      
        1 gaacagcgtg cgcggccgct cctcgtcggt gcagatgtag accgtggccg gcacctgttc
       61 gaccagcacg cgatagcgcg tctcggaccg gcggagatcc tcttcggcca gcttctgcgc
      121 ggtgatgtcg agcatgacgc cctgccagaa gagggtgtcg cccacctcgt ccatgatcgg
      181 gatcgaactg tcgcggaccc acagcgagcc accgtcgggt cgcaccatgc ggtactcgag
      241 ggtgaaggga tctcggctct cgatcacgcc ggcccacgcc tgcttgacgt tcgtcaggtc
      301 gtccgggtgg atcgtggcgt accagagccc agggtcctcg aggtagcgcg acggcgcgta
      361 gcccaggacc tcctcggagt tcggactcac gtacaggctg agcgggtcga cctcgtcgga
      421 gtccacatag accacggcgg gaaggcgttc gaccagggcg cggtaccgag cctccgagcg
      481 ggtgagcgct tcctcggcgg cctggcggtc ggtgatgtca tgggtgacgc cctgccagca
      541 gacgacacca ccgtcgaggt tgcggaccgg gtcggtggcc tcgcgcaccg atgccaccgt
      601 accgtcgggt cgaacgatcc ggtactcgca caagaacgag gaggcggcac gcaccgattc
      661 cgcccatcga tccatcacgc gctcctggtc ggccgggtgc atcgaccgca tccacaggtc
      721 gggatcttcg agccaggcct ggcggaaata gccggtcagc cgttcgacct gcccgttgac
      781 gtagaggagc cggggcggca cctcgttcgt caggatgaag acggtggcgg ggatgtgctc
      841 cagcaggtcg gcgacccggc gaccggcgat ggcgtcggcc tgcggatcaa agggctctcg
      901 ggggtcgtcc gccatggccg cagcagccta ccccggcggc cacgacctcg ggaagggccc
      961 gttcagggca gggtctcgcg gtagatctgc atctcgaact cgatgaaccg cgcgaccatg
     1021 acgcggtaga catcctcggc gatgtccggg ctcatcccgt actcggtggc gaggcctcga
     1081 acgttgctca cgacctcttc gacgcgctcc ggcacctcga tctcggcggc ggagttcttg
     1141 atcctcgccg cctgctcgat gtagccgctt ctctccgaga gcagcccgac gatctgtcga
     1201 tcgatggcat cgatgccctc gcgtacctca tcgaggttct tgcactcctt cacatcgatc
     1261 cacctccgca acgttgggcg cttcccgagt atcaccgatg gaggtgctca gcgggttcga
     1321 ccgacgcgac gccaccggcg agggtggggg cgtggacctg ttctcccgat aggctcatcg
     1381 acgggaggat ggccgagcgg acgaaggcga cggtcttgaa aaccgttggt cccggtgacg
     1441 ggtcccgtgg gttcgaatcc cactcctccc gcttcggaat tgcggctgcg ctcaggagcc
     1501 gcgcgcggcg tgcgctacgc ggtgcccagg tgttcgtcga gcaccgagag catcggccag
     1561 gcgccgtcgg accagttcgc gatagctgag tgggtgatgc ccgacaccgg gtcgtgcgtg
     1621 gtgcagaccg agacgccggc gtcgtagccc tcgagccaca cggcctcgtt cgtgcgatgc
     1681 aggtggaacc cgagcccgta gcggcggtct tcttcgggcc actcgctgcg cgggcgggtc
     1741 atctcggcca cgagctcggc gggcacgatg cgaccgccgt agagtgcgcg ccagaacgcg
     1801 gcgagatcgg cggcggtcga ggagatgccg ccgtctccgg tgccgagcac gggcaggtgg
     1861 agcgcgttcg atcgctggcc ggccgcgtgc aggtacgcga tcgcatggtc gcccgggagc
     1921 tcgtccgtgc gccagaattc ggtctctgcg agcccggccg gttcggtcac gcgctcccgt
     1981 acgagctcgt ggtagcccac cccgctggct cgctcggcga tgagcgcgag cacgacgtaa
     2041 ccggcgttgt tgtaggagaa ccgctcgtct gcgcgagaga cggtcgggaa gccgtcgagc
     2101 acctcgagga accgctcggt cgtgtcgagc cggtgcacag gcacgggtga cgtgtaggag
     2161 ttgatgtcgt cgtcccgctc gtcctcgtcg aggtagtcgc cgataccgga tcggtgcgcg
     2221 agcaggtgct cgacggtcac gtcgtcggcg atcagcggca ggtcgtcacc gagcaccgag
     2281 cgtgcggtcg tcgagagctg gagggtgccg tcggcgacga ggctcatgac ggccagcgca
     2341 gtgaagcctt tcgcgacgct cgccagcatg aaccgcgtgt cgaccgtgtt cgggatgccg
     2401 tgggcgcgat cggcgagccc gtaggccttc tcgagttcgg tggcaccgcc tcggtccacc
     2461 cgcacgacac cggagaaggc cgtacgctcg gccgctgcgt ccaacgccgc ttccaacgcg
     2521 tccatcgcag gaccgtagca gcgacggctt aggtcgggat cgaatcccac tcctcccgct
     2581 caggacccga ccgaggtggt cacgatccgg ccgtcggcca tcgatagcgt gcgatcggcg
     2641 aaccgttcga gcgtcccgtc gtgggtggcc acgatgcaac aggtacccaa cgaagccgcc
     2701 tcctgcagcg tggcgaagat cttcgagacc cacgccgcat cctgatggcc ggtcggctcg
     2761 tcggcgatca gcacctgcgg tcgcatcacg agcacgcggg ccagtgcggt gcgctgttgt
     2821 tcaccgaggg aggtctcgcg gggagcccga tcccggagct cctcgagccc gaaccggcgc
     2881 aggagcgagg ccaccagctc gcgacggtcc gcgaggtcgc cggccaccct cgccggatac
     2941 tcgacgttct cctgcacggt cagctcctcc atcaggccca ggcgctgagg aaggacggcg
     3001 atctcggacc agtccggctc gcgcccacca ggggcgctga cgactccttg gtcggggatc
     3061 tcccagccgg cgatggagtg cagcaacgtc gtcttgcccg agcccgaccg gccgacgagc
     3121 gcgatcacct ggccgacccg cgcctcgagg tcgacgtcca ccagcgctcg aaccgcttcc
     3181 tccccgtgtc cgtaggtctt cgagacgccg acgagatcga ccacgggacc gtcgtcccag
     3241 agcggctcca tcgacgtcgg ccaccggagt tcatcgagct gacggtcctc ccattccccg
     3301 ccggcatccg tcacgctgcc gcggacgctg ccgtgatcca gcgtcagggt gtgatccgcg
     3361 acggccacca ccgcggggtc gtgggtcgcg atcacgaacg agatgccgcg ttccgcgagc
     3421 tcgcggatgc gctcgagcac ggcggcaccc gacggtccat cgagttcggc cgttggctca
     3481 tcggcaacca ccagcggcga accggtcgcc agggcctggc cgaaggcggc acgctgctgt
     3541 tcgccgccgg agagttctcc ggggaggttc tccgctcgat ggccgatccc gagcgcgtcc
     3601 aggagctcgg cgaggtccgc cccggcgggc gagcgaccgg ccgccatccg cagatgggct
     3661 cccacggaca ggtagggcac gaagttgtcc gacggtcgct ggaacacgta gccgaccttc
     3721 gagcgacgga ggtggcgtcg agctcgcgcc gacgccgagg tgacgatcgt gccgtcgacc
     3781 tccaacgtcc cggcgctcgc tcggtccagg ccggccatca gtcgcaggag cgaggacttc
     3841 ccgctgcccg aagggccgac gaccgcggtc acggcgccgc gcgtgaatgt ggcggagacc
     3901 ccccgcagtg cctgtacctc accggtgggc gtgcggtagg tccgcacgag gtccgtgcag
     3961 cgcgcgatat cgacggcgtc attcggcaac tctgagcacc tcccccaggg gcacccgtcg
     4021 agccgaacga tcggccagca gcgcaccggc gacggaggcg atggcggtcg cgagcaagcc
     4081 gatcgcgatc ccgacgacgg gcagcacgat cagcgaatcg ggtgggatcg tgggcaaggg
     4141 atcgagcctc ggcgccacga gcctcgtggc cacggcaccc accaccgcgc caaccaccag
     4201 ggcaccgatc aggaccgttc cgagctccag caccagggag cggcgcatcg tgccatccgc
     4261 ttgtcccatg cggccagaga gttcggtcgc caccacgcgg gagcgctgcc gggcttggag
     4321 gtagatcatc gcgacgacga ccagcagcac catcgccacg atccccagga tgtccaacat
     4381 caggaacgtc tggatcgcgg catcgatgaa cgggatgtct tccacctcgt cggccgtcaa
     4441 cacgctgtac ggcgtcgcgc cgatgtcggc gagagacgcg aggatctcgt ccgtgggccc
     4501 cgcgacccag aactccgccg aggccatcgg ggtgttcagc ggatcgggga tgtccgtgaa
     4561 gtgctcggcg agggcatcgc gatcgacgac caggagcggt ctgtccgatg tggacgtgcc
     4621 ggggaacgac gtggcccggc ccaccatctc gatcggaacc gtcttgtcgc tgaggtcgat
     4681 cgaggacggg tcgtagtccc cgccgttcga caggatgacc ggcagcgcgg cgccccccgt
     4741 cgacgggagc cgggtaacga ggtcggccag ggagtcgtcg gagagggcgt cgttccagaa
     4801 cgccgccgcc tcgaacgtgt ccgggtcgat cgccagcagg tcgtagcgca gcgtgctgcc
     4861 ggtgaacgct cccgcgtcgc gcgaccgcac cacacgggtc acggggaagc cgaaccccgg
     4921 cgcgggctcg atgtcgcgac tcacgaccac ctgcacgtcg ctgccgacga acaccttggc
     4981 cttcgcgtcc acggtggtgc ggagcgaatg caccatgccc tgagaggaca cgaacacggc
     5041 aagggtgagg cttccggcga tcagcaggag ttgcggcagc cggcctaagg ttcggaggcg
     5101 tcgggacgcc aaccaccacg gccctgcgcc ttgggagccg ctcgcgcgtc ggtggccgat
     5161 cgtcagcgcg atcgtcatca cccgagccac gaggatcccg atggccaagg ccagcagcaa
     5221 cgggaagagg aagacggcgg cgcccggtcg ctcgatccct ccggactcca cgatggcccc
     5281 gccgccttcg aggcgcctgg ccatcaccca cgctgccgcg aaggccgcga gctcccaggg
     5341 aacgaacccc aggacccggg tgagccgatg ccggtgatcg tggctggaga ggaacacggc
     5401 ggccgagacg aggcccacga ccagcaggga ggccgcgacc gcgatcaccg cggcggtgac
     5461 cgccgtcgat cgcgcggagc tctcgatcct gcccggcgga cccacggccg cgatcgtgac
     5521 gctcgccagg gcgaatccga cgatgccccc ggcgaggaac ggcaggacgg cctcgagcac
     5581 ggctttcgct ccgaccgcgg agcctcccca gcctcgcacc gacagcacac cgacctcgac
     5641 gcggcgcgac atcaagacga acaccccggc ggccgcgacg atggcgagtg agatacccag
     5701 cccggcgatg agtaggacca ggagcggacc ttgcacggag atgaggcgct cgtcgacctg
     5761 tccgacgacc tggccggcga gagatgtgta ggacacgtcg ctcgcgggac agaagaactg
     5821 gacgcagttg ctctggatga agacgtcacc gcagcaacgg aagagccgga acagctcggt
     5881 gtccttcgtg ctcatgtccg cctcgaaccg gtcggcgaac cccgcgaggt cgcgcgcctc
     5941 gtccagcgtc aacggtggat cggccgacgc cggcgccacc aaggcgaacg tcccctcggg
     6001 gtcgccgaga cgcgtcccga gatccaacag ctgcgaccgg tcgaccagga tgggctgggg
     6061 cgggaggggg cattcgtagc ccggacagat cgcatagatg tcgtcggccc aggtccgcca
     6121 gtaccccgat cgcggctggg tgtacagcgc gcgatagatg ccgtccaggg tcacggtctc
     6181 gtggtgtccg tcgacggtga gttcgaattc gtcgcccgga cgcaggcgcg ccccgcgagc
     6241 tagcaggtcc ggcaaccaca cgcccggacc gtcggcaccg gcgaggatcg tgacgttgtc
     6301 gaggacgttc gtgccggcga acaggcggcc cacgacccct cctgaaggag gcgtgcgccc
     6361 gccggggagg gtgacgtcca ccgtggttcc caggatctgc tcttgcgcgg gttcaagctc
     6421 cggactctca gcggtgcgtt cggcgaaggc ggcggctcgc tcgtcgatca atgagccgcc
     6481 tcccggcgcg cgcgtcgtga agttcactcg cgtggcgcga taggtgatcc cggtgccgta
     6541 gttcgtgacc agcggatcgg cgatctgctc ggcgagcagc tggctcttgg acgcggacag
     6601 gaacaagggg taggacgccg ccgtgatcga caccaggaac gcgctcagtg ccagggcggc
     6661 caacagagcc ggcgagtgcc gcaaggccgt cggtgcccgg cgccatatcg gacgtcccga
     6721 ggggatcgtc ggtgttcgcg tatcggtgcg ggccacctgg ctccttctga cgggcggact
     6781 cctgaatgta cgaaagccgg gtcgattgcg ttgcgcatcg cccggccttt ctgggactca
     6841 gggaccacgg ccgagacacg gcccgcggtg caagggaaag ggcgaagcgt gagcgagggc
     6901 tatcacctca gtcggcgaat gttcgtacgg ctctcggcag gggcggtggc agcgaccgcg
     6961 ctccccatcc ccaaggccct cggcagcgaa cggggcggca cctgcgatcc gttctcgacg
     7021 cctccgagct tccagggcgt ggtgccctcg cccgaggatg tcctgggctt cccgatcggg
     7081 gtggatcggg agaccaccgc cggcgagatc aacacctacc tggacgccgt cggcggggcc
     7141 agtaaccggg tgcgcgtgcg gacgctcggc gattcggtcc gagggcgccc gatccgctac
     7201 gcgctcgtcg gcgatcccgc gaacgtcacc aaccaggggt tggcgcagat ccaggccgac
     7261 accgccacga tccgtgaccc cgccacgccg cagacgacgg tcgacgccct ggccggcacc
     7321 acgccggtga tcctgtggat cgcggggaac ctgcacggcg gcgaggagag cggcgccgac
     7381 gcctcgctcc agctgctgta cgagctcgcc gaccgcgacg actgcggggt gaccgacatc
     7441 ctggctcgcg ccctgatcgt gatcatcccg acgcagaacc ccgacggacg ggagatcggg
     7501 ttccgccgga acgggttcgc cttcgacttg aaccaggacc atctggtgcg gacgcaggtg
     7561 gaaaccgagg gcaagctggc gttgatgaag cagtacccgc cggcggtgct caccgaccac
     7621 cacgagttcg ggtactaccg ttcgttcttc ccgccgaacg acgaccccgt ctatcacgag
     7681 acgccggaga acacggtccg ccagatctac gacctgttcg gtcccgcgtt cgctcgcgag
     7741 ttccgccgga acgattggca gttcttcaac gaggggtacg gctacgacct gttcgcccca
     7801 cggttcacgg acacgatcgc cgtgatggcg tatgccgcgg cggggatgac gatcgaggtc
     7861 tacgacggtg cgccgctgga ccgccggttc gcccggcacc tcaccgtgat gtggtcgatg
     7921 atcgggacgg gtgccgcgaa ccggaagcgg ttgcagcgga acctgcacga gtcctacgtc
     7981 caggcgctcg atgaggggcg tcgcggcgag ctcgagccca accgcacgta cgagccacat
     8041 tccaggccgg tgcgcagggt gcccgaccag aaggtgcggc actacttctt caaggacgag
     8101 ccggccaagc gtcgcgagat cgcgatgctg atccaccggc tgcaggagat ggacgtcgcg
     8161 gtctaccagc tgaacgctcc gttgcgcgtt ccagatctga aaccctacct ggagtcaccg
     8221 cacgccggga ccgtgccggc gggcacctat tggatcccca tggcgcagcc acagaagcgc
     8281 ttcatccagg cggcgctgaa cgaggatccc tacaacccga ccaaccaggc gtaccgggtc
     8341 agctcgtgga gcctgccgct cgcctacaac ctcaccggct ggctgtcggg agcccggttg
     8401 gatccggacg ccgatcccgt ggctccggtc ggcgaacccg ggccgcccac cctgccgcag
     8461 cctctccccg ccgtgcggat catgcacctg tcccgggaat tcaccgccta cgaggagatc
     8521 ggacacacgc gctggctctt ccggcggagc tgggaactgc cgttcacgga gatggacgcc
     8581 gacgaggtgg ccgcgggcat cgacggcgtc gacgtcttgg tcgtgccggc gggcgggatc
     8641 aacgagggtc tgcgccggtt gggcaaggcc ggccagcgcg agttgatccg ctgggtgaac
     8701 cacggcggcc ggctcgtcgc ctggcgctac ggtgccgcgc ggttggccta cacgctgggg
     8761 atctccaaag cggtctacga catggtgccg gccagcatcg acggtccgat gctgaaagcg
     8821 aacatcgacg ccgacagccc gctgggtgac ggcgtggggc ggcacgcatg gcccctggtc
     8881 gacaccaggg cgatgcacgc tcccgacgcc gtgtcgccga tcacgttccc cacgcaggcg
     8941 agcggtgact ttcgggtgtc gggggttcgt cgaggcacgc gcttcctgat gggcagcacg
     9001 gccgtggccg acgaaccggt gggtcgcggg cgctcgatcg tgttct
//
LOCUS       DATDBZ010000482         4793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_3021694, whole genome shotgun sequence.
ACCESSION   DATDBZ010000482 DATDBZ010000000
VERSION     DATDBZ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3021694"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VIX62_11295"
     CDS             <1..1765
                     /locus_tag="VIX62_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain containing protein"
                     /protein_id="HEY5860862.1"
                     /translation="NYLFSFDATTGAVDPNFRPSLNKAVEALAPGPDGTSVYVGGRHT
                     TINGSSNFDRLSRLRLSDGGLVTSFAPNPNGNVYDLVGRNSRLYAGGAFTTIGGQART
                     RLAALDLTTGAADTSLNIPFTGNPIGGTSQVWKFDITPNGSTMVVTGNFDTAGGQPRS
                     QIAIVDLSTNPATLSSWSTDLFPFYDTSVVPPRTWCAGVFPHWIREIDISPDGTYVAI
                     VTTGGNYPNRLCDSVTRWELGRTGPGQTPTWIDSTGGDSFHSVLATGTAIYAGGHQQW
                     VNNPYNPTRCGVCTGPFPGGMVRTGFSAHDPLNGLPFRWNPGRNPRGKGVLAMVATPT
                     GFFFGSNTDRIAGEYRRKLAFMPLAGGTTVPSIAMPTLPGGFFSAAPTGAPTTMVRRS
                     YNGTTFGAPGTVATGVDWSDTRGAFALNGRLYQARSNGTLTVRSFNGTTAGNATAIDL
                     HGLQTAPDPVVYRIPGTALPIPGLGTHLSTMTGLAYEGGWLYYTVQGDPRLYSRGFTE
                     ESQTVGAPLLVASSGDGVNWANVRGLAIASGNLYFALADGTLNRIAWVNGHPSGAATT
                     IGGPAIDGTNWASGGLFVFGS"
     gene            1805..2635
                     /locus_tag="VIX62_11300"
     CDS             1805..2635
                     /locus_tag="VIX62_11300"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5860863.1"
                     /translation="MARLSTRDRAKLPDKAFAYIDADGHRRLPIHDEAHVRAALGRFE
                     RIRFEDDAARERARRRLLNAAKKYGIVPVGFITGQLRSERSSRSPDFSTFPTGTVTFL
                     MTDIEGSTLLLRELGEAYAAVLKDVRATIRSAVRRCGGHKVDAIGDEYFSVFERAAPA
                     IEAAMDLQVAMAQRTWPGERPVRVRAGIHTGRPTLTDGGYVGLAVHTTARICSCGHGG
                     QIVASAQARKAAQGSMPAGARLRSLGSHRLAGLGQPETLFQLHADGLPSRFPALRTGP
                     "
     gene            2740..3114
                     /locus_tag="VIX62_11305"
     CDS             2740..3114
                     /locus_tag="VIX62_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860864.1"
                     /translation="MADDAKQAWNDVGERFASLGNRLSDKYRESGSGGEPDAAETQRK
                     LQETAKEVGDQLSRAFDALGATIRDEEAKADLKQALNAFGDALGATFDEAGHAIRRTV
                     GSSGETPPAKPDDPPTGPANDG"
     gene            3107..3514
                     /locus_tag="VIX62_11310"
     CDS             3107..3514
                     /locus_tag="VIX62_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5860865.1"
                     /translation="MADRANPRDALAAIPLFAHLSSRQLRRIASSTNEDAYEAGDVII
                     NEGGRSQSMFVIVEGTASVVRDGQTLAQFTPGQFFGELSMIDERPRAASVVADTPMRC
                     LVLKHEVLRDIVMSDPQVAWSLLKTLASRLRDD"
     gene            complement(3524..4591)
                     /locus_tag="VIX62_11315"
     CDS             complement(3524..4591)
                     /locus_tag="VIX62_11315"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY5860866.1"
                     /translation="MPSAPAHRRLVIAAVSAAIAVAGIVQVPASAQTPAKAAERCFEP
                     DVPERFQRHVARAIRVSRDLPREWADSPFIARIVCWQGTDFDPDFRKRGGANFVWHGQ
                     FAMTTQEMATVFGPWMTANRYAFRLSPSCFVHGWEACPNTTANAAVIQQLIAGMRWIW
                     LNYGTPTAAWGHIQRTGRFTSYPRTGTDNDPTRDPFRRCPVGGGVHYSDDFGERRTVG
                     GYHPHWGNDVIAPTGRPVRAPFDGFAVPHSDGWFAGRWVTVVGPNGYVRNDHLSRFAK
                     RGVVKAGDIVGYVGATGDATGPHDHFEWHPWELPTPLHRSRFGFTRVMDAIDPYPFLN
                     KACGARRIPAPEGFPKQPLEG"
BASE COUNT          768 a         1709 c         1622 g          694 t
ORIGIN      
        1 caactacctg ttctccttcg acgccaccac gggggcggtc gatcccaact tccgtccctc
       61 gctcaacaag gcggtcgagg cgctcgcgcc cgggcccgac ggcacgtcgg tctacgtcgg
      121 tggccggcac accacgatca acggcagcag caacttcgac cggctctctc ggctgcgcct
      181 gtccgacggc gggttggtga cgagcttcgc gccgaacccg aacggcaacg tctacgacct
      241 cgtgggccgg aacagccgcc tgtacgccgg tggcgcgttc acgacgatcg gcggccaggc
      301 tcgcacgcgc ttggccgcgc tcgacctcac gaccggtgcc gccgacacct ccctgaacat
      361 cccgttcacc ggcaacccga tcggcggcac gtcgcaggtc tggaagttcg acatcacgcc
      421 gaacggcagc acgatggtgg tgaccggcaa cttcgacacc gccggcggtc agccccgctc
      481 ccagatcgcg atcgtggatc tgtcgacgaa ccccgccacc ctctcgtcat ggagcaccga
      541 cctgttcccg ttctacgaca cgagcgtcgt gccgccgagg acctggtgcg caggggtgtt
      601 cccgcattgg atccgcgaga tcgacatctc ccccgacggc acctacgtcg cgatcgtgac
      661 caccggcggc aactacccca accggttgtg cgactcggtg acccggtggg aactcggccg
      721 caccggcccg gggcagacgc cgacgtggat cgactccaca ggcggcgaca gcttccacag
      781 cgtgctggcc accgggaccg cgatctacgc gggcggacac cagcagtggg tcaacaaccc
      841 ctacaacccc acccgatgcg gggtgtgcac cggcccgttc ccgggtggga tggtgcgcac
      901 cggcttctcg gcgcacgatc cactgaacgg gctgccgttc cggtggaacc cgggccgcaa
      961 cccccgcggc aagggcgtcc tggcgatggt cgccacgccg acggggttct tcttcggcag
     1021 caacaccgac cggatcgccg gggagtaccg caggaagctg gcgttcatgc cgctggccgg
     1081 cggcaccacg gtcccctcga tcgcgatgcc gacgctgccg ggcgggttct tctccgcggc
     1141 acccaccggc gcccctacca ccatggtccg tcgctcgtac aacggcacga ccttcggcgc
     1201 accgggaacg gtggcgaccg gcgtggactg gagcgacacg cggggagcgt tcgcgctgaa
     1261 cggtcgcctg tatcaggcga ggagcaacgg caccctcacg gtgcgatcct tcaacggaac
     1321 gaccgcgggg aacgccaccg cgatcgatct gcatggactg cagaccgcgc ccgatcccgt
     1381 cgtctaccgc atcccgggaa cggcgctgcc gatccccggt ttggggacac atctgtcgac
     1441 gatgaccggg ctcgcgtacg agggcggctg gctctactac acggtgcagg gtgatccgcg
     1501 cctctactcg cgaggattca ccgaggagag ccagaccgtg ggagcgccgc tgctcgtggc
     1561 cagctccggc gacggggtga actgggcgaa cgtgcgcggt cttgcgatcg cctcgggcaa
     1621 cctctacttc gccctcgccg acggcacgct gaaccgcatc gcctgggtga acggtcaccc
     1681 ttcgggcgcc gccaccacga tcggcggtcc ggcgatcgac ggcacgaact gggcctcggg
     1741 agggctcttc gtcttcggct cttgaacgag ggccggtgtc gccggctaca ggtatcgtcg
     1801 ttccatggct cggctcagca cccgtgaccg cgcgaagctg cccgacaagg cgttcgccta
     1861 catcgacgcc gacgggcacc gacggctgcc catccacgac gaggcgcacg tgcgggcggc
     1921 gctcggccgg ttcgaacgga tacggttcga agacgatgct gcacgcgagc gtgcacgccg
     1981 ccggctgctg aacgccgcca agaagtacgg catcgtgccg gtgggcttca tcaccggcca
     2041 gttgcggtcc gaacgcagct cgcggagccc ggacttctcg accttcccca ccggcaccgt
     2101 caccttcctg atgaccgata tcgaaggttc cacgctgttg ctgcgcgagt tgggcgaggc
     2161 ctacgccgcg gtgctcaagg acgtgcgtgc cacgatccgc agtgcggtcc gtcggtgtgg
     2221 cggtcacaag gtcgacgcca tcggcgacga atacttctcg gtgttcgagc gcgccgcacc
     2281 ggcgatcgaa gccgcgatgg atctccaggt cgctatggct cagcgcacct ggccgggcga
     2341 acgccccgtc cgggtgcgtg ccggcatcca tacgggccgg cccacgctga ccgacggggg
     2401 ctacgtgggt ctcgccgtcc acaccaccgc gcgcatctgc tcgtgcggcc atggcggtca
     2461 gatcgtcgcg tctgcccagg cgaggaaggc ggcgcaggga tccatgccgg ccggtgcgcg
     2521 gcttcgcagc ctcggaagtc atcgcctcgc cggtttgggg caaccggaaa cgctgttcca
     2581 gctccacgcc gacggtctgc cgtcacggtt ccccgccttg cgcaccggtc cgtgagccgc
     2641 atcgcgggga ccccgcctgg acgctcgaac gcagagcgtg cagactcgga accgcgggga
     2701 acacccgtgg acgaacaccg acatcaggga gcgatcgcga tggctgacga tgcgaagcag
     2761 gcatggaacg acgtgggaga gcggttcgcc tccctcggca accggttgtc ggacaagtac
     2821 cgcgagtccg gttcgggcgg cgagccagat gccgccgaga cccagcgcaa actgcaggag
     2881 acggccaagg aggtcggcga tcagctctcg cgtgccttcg atgccctcgg ggccaccatc
     2941 cgggacgagg aagccaaggc ggacctgaag caggcgctca acgcgttcgg cgacgcgctc
     3001 ggagccacgt tcgacgaggc cggacacgcg atccggcgaa ccgtgggctc cagcggcgag
     3061 accccgccgg ccaagcccga cgatccaccg acgggcccgg cgaacgatgg ctgacagggc
     3121 gaacccgcgg gacgcgctgg cggccatccc gctgttcgcg cacctgtcca gccggcagct
     3181 ccgcaggatc gcctccagca ccaacgagga cgcctacgag gcgggcgacg tcatcatcaa
     3241 cgagggcggg cgctcccaat cgatgttcgt gatcgtggag ggcaccgcca gcgtcgtacg
     3301 cgatggtcag acgctcgcgc aattcacccc gggacagttc ttcggcgagc tgtcgatgat
     3361 cgacgaacgc ccacgagcgg cctcggtggt ggccgacacc ccgatgcggt gcctcgtgct
     3421 caaacatgag gtgctgcgcg acatcgtgat gagcgacccg caggtggcgt ggtcgctcct
     3481 gaagaccctg gccagccgcc tccgcgacga ctagccgtcc cgctcagccc tccaagggct
     3541 gcttcggaaa gccctcgggc gccgggatcc gcctggctcc acaggccttg ttgaggaacg
     3601 ggtaggggtc gatggcgtcc atcacacggg tgaacccgaa ccgcgagcga tgcagggggg
     3661 tcggaagctc ccagggatgc cactcgaagt ggtcgtgcgg gccggtcgca tcgccggtgg
     3721 caccgacgta gcccacgatg tcgcccgcct tcaccacccc gcgcttggcg aaccggctga
     3781 ggtggtcgtt ccggacgtat ccgttcggcc cgacgacggt cacccatcga ccggcgaacc
     3841 agccgtcgct gtgcggcacg gcgaagccgt cgaacggcgc ccggaccggg cgaccggtgg
     3901 gcgcgatcac atcgttgccc caatgcgggt gatagccgcc caccgttcga cgttcgccga
     3961 agtcgtccga gtagtgaacg ccgccgccca cggggcaccg gcggaaagga tcgcgggtcg
     4021 ggtcgttgtc ggtgcccgtg cgaggatagg aggtgaagcg tccggtgcgc tggatgtggc
     4081 cccaggcggc ggtgggcgtg ccgtagttca accagatcca ccgcatgccc gcgatgagct
     4141 gctggatcac cgcggcgttc gcggtggtgt tgggacaggc ctcccatccg tggacgaagc
     4201 acgacgggga gagtcggaac gcgtaccggt tcgccgtcat ccaggggccg aagacggtcg
     4261 ccatctcctg ggtggtcatc gcgaactgcc cgtgccacac gaagttggca ccgccgcgct
     4321 tgcggaagtc cggatcgaag tccgtgccct gccagcagac gatcctggcg atgaacgggg
     4381 agtcggccca ctccctgggc aggtctcgcg agaccctgat cgcgcgggcg acgtgccgct
     4441 ggaagcgctc gggcacgtcg ggctcgaagc agcgctcggc ggccttcgcg ggggtctgcg
     4501 cggacgccgg cacctgcacg atcccggcca ccgcgatcgc cgccgacacg gcggcgatga
     4561 ccaggcgacg gtgcgcgggt gccgaaggca tggcgcgtat cctacgtcta ggctgccggg
     4621 cgatggtcat gccccgagcg atcgccgccg ccctgtcgat actcgctctc gccctcgcgg
     4681 gatcgtgcac cgatgagccg ctcgaaccac cgcccacggc ggcctcgagc cctccccccg
     4741 cctcaccgct ccccacccag ggcgtggtcg ctcgcgacac cttcgatcgc ccc
//
LOCUS       DATDBZ010000483         5238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1382284, whole genome shotgun sequence.
ACCESSION   DATDBZ010000483 DATDBZ010000000
VERSION     DATDBZ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382284"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1160)
                     /locus_tag="VIX62_11320"
     CDS             complement(21..1160)
                     /locus_tag="VIX62_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY5860867.1"
                     /translation="MSEITDVLAAIESMSAQGQKMALATIVAVRGSTYRRPGARLLVP
                     EEGAPIGNISGGCLEGDVADMARVVMDEGTARLAGWDLTADDDAVWGLGLGCNGAIEV
                     FIEPAEEAALVASALRAALEEDRPICVITAIESEDAAVEPGARIVVRPDTEAGPDGSL
                     GSATLDAEAVAAARELLAAERSEVRTLTGGVRAFIEVLEPPLRLLVCGAGHDAIPLTR
                     AAAGLGWNAIVVDDRPAFLTADRYPDAGGFVHVEAPELTAKTTPIDAHTFAVVMTHNF
                     LRDKEYVRSLLDSPAAYIGMLGPGARTERLLMELRDEGVTITDEDRARIHGPAGLDLG
                     AEGPEEIAQSIVAEIVAVKRARGGGFLKERPGPIHDRPAPGTEAR"
     gene            1249..1815
                     /locus_tag="VIX62_11325"
     CDS             1249..1815
                     /locus_tag="VIX62_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860868.1"
                     /translation="MDDTFSYRAAPLPLRRRIDPRSVKLVVVAIVVVSCCVLFTKWVI
                     DSERRSEVLASGHAAEAPLVGHFEGIDAEAAAAVPALPVVDAPARADARTALATAREA
                     ARGRSTFADAGPGQLSALERSLIFTDGPSPAPGIVSVAASGGRWAGAVMGTSGTCYWV
                     RLGHTGTTFGSGELCTGFAALSADDAAW"
     gene            1926..2402
                     /locus_tag="VIX62_11330"
     CDS             1926..2402
                     /locus_tag="VIX62_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009604353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5860869.1"
                     /translation="MTHVSVTVNGEQREADVEARTLLVYFLRETLGLTGTNVGCDTST
                     CGSCTVMVDGESVKSCTMLTAQADGRAVTTIEAMADGDTLHPLQEAFHRNHGLQCGYC
                     TPGMIMAAASYLKENPAPTEEEVRESLEGNLCRCTGYQNIVKSILDAAGSMSGAKA"
     gene            2416..4773
                     /locus_tag="VIX62_11335"
     CDS             2416..4773
                     /locus_tag="VIX62_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY5860870.1"
                     /translation="MVATETFIGQPVLRKEDGDLLTGQARFIDNFTMPGMLWMAMVRP
                     PYVHATINSVDTSAAASMPGVVGVYTGKDLSLGALPFVWPITEDIKIPVHYPLATDKI
                     RFAGDAVAVVLAETREQAEDAAEMVSIEASELKAVTDLREAAKDEVIIHDDLGTNVVV
                     HWSHGGAGDQDIFESAPVIVQETYDQPRLIPNAMEPRGCLAYGVGAMDEWTLVSATQI
                     PHIAKVTLAGVVGIEEPKFRVIAPDVGGGFGSKLNVYAEEALACALAKRSGRPVKWIE
                     GRQENYVATIHGRGVTHDCTLAGTEDGLILGVKFVELADMGAYFQLLTPGIPELGAWV
                     YMGPYRPETYWYEFRGIVTNCTPTDAYRGAGRPEATYVLERLVDAFARKIGKDPADVR
                     RMNFHPPFDEATTSICGLNVDSGNYEPLLDKALELSAYDEVRKEQQARRDRGDTKQLG
                     IGLSTYIEMCGLAPSNILGALRYAAGGWDGATIECTPGGGVIVKTGTSPHGQGHETSW
                     SQIVADGLGVDPDKVEVLHGDTAITPLGMDTYGSRSLSVGGVALHFAMEKIKEKARTI
                     AAHELEVSEDDLEWADGSYRVKGAPDKARTIPDLAVSAWHAHDLPAGVEPHLNATAVY
                     DPPNFTWPSGAHIAVVEVDTETGATDIIKYIAVDDCGTVINPMIVEGQVHGGVAQGVA
                     EALYEEAIYDEGGNLTTSSMTQYLVPTAVEIPAIITDNSQETPSTTNPLGVKGIGEAG
                     TIASPPAVINAVVDALSHLGVTEVGKPASPERVWRAIQDARGGAA"
     gene            4770..>5238
                     /locus_tag="VIX62_11340"
     CDS             4770..>5238
                     /locus_tag="VIX62_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY5860871.1"
                     /translation="MIPAPFEYARAESVDHAIELLGSSEDAKILAGGHSLLPLMKVRF
                     AQPSLLVDIGRIGDLSYVREDDDVIAIGALTRHQDVANSELLQASCPIVADAASQIGD
                     PQVRHMGTIGGAVAHSDPASDMPTVMLALGAQMVVRSAGGQSRTVAAADFFQGL"
BASE COUNT          848 a         1799 c         1824 g          767 t
ORIGIN      
        1 aaagaacggg caactcaggt tcagcgcgct tcggtccccg gtgcgggccg gtcgtggatg
       61 ggacccggcc gttccttgag gaagcctccg ccgcgggcgc gcttgacggc gacgatctcg
      121 gcgacgatgg actgcgcgat ctcctccgga ccttcggccc cgagatccag cccggccggg
      181 ccgtggatgc gcgcccgatc ctcgtcggtg atcgtgacgc cttcgtcgcg gagctccatc
      241 agcagtcgtt cggtacgagc gccgggaccc agcatgccga tgtaggcggc cggcgaatcc
      301 agcagcgatc gcacgtactc cttgtcccgc aggaagttgt gggtcatcac gaccgcgaac
      361 gtgtgcgcat cgatcggggt ggtcttcgcc gtgagctccg gtgcctcgac gtgcacgaac
      421 ccgcccgcgt ccgggtagcg atcggcggtc aggaacgccg gacggtcgtc cacgacgatg
      481 gcgttccagc cgaggccggc cgcggcgcgg gtgagcggga tggcgtcgtg gccggccccg
      541 cacacgagca gacgcaacgg gggttccagc acctcgatga aggcccgcac cccgccggtg
      601 agcgtccgca cctccgagcg ctcggcggcc aggagttcgc gcgcagccgc cacggcctcg
      661 gcatccaggg tcgccgatcc cagggaaccg tcgggcccgg cttcggtgtc ggggcggacc
      721 acgatccggg ccccgggttc gaccgcggcg tcctcagact cgatcgccgt gatgacgcag
      781 atcggccggt cttcttccag cgcggcgcgc agcgcgctcg ccacgagcgc ggcttcttcg
      841 gccggctcga tgaacacctc gatcgccccg ttgcagccga gccccagccc ccacaccgcg
      901 tcgtcgtccg cggtgaggtc ccagccggcg agacgggcgg tgccctcgtc catcaccacc
      961 cgggccatgt ccgcgacgtc gccctcgagg cagccgccgc tgatattgcc gatcggcgcg
     1021 ccctcttcgg ggaccaagag ccgagcgccg gggcggcgat acgtcgaacc gcgcacggcg
     1081 acgatcgtgg ccaaggccat cttctgaccc tgggcggaca tcgactcgat cgcggccagg
     1141 acgtcggtga tctcgctcac ccgccgatgg tatcccggcg gccacgaacg ggacgttcga
     1201 cggccggggt ataggtcgag cgttcacgcg ccgatacctg cggcggggat ggatgacacc
     1261 ttcagctacc gtgcggctcc gctgccgctg cgtcgccgca tcgacccgag gagcgtcaag
     1321 ctcgtggtgg tggcgatcgt ggtggtgtcc tgctgcgtgc tgttcaccaa gtgggtgatc
     1381 gacagcgagc ggcgcagtga ggtccttgcg tccggacacg ccgcggaagc gccgctggtg
     1441 ggccacttcg agggcatcga cgccgaggcg gccgcagcgg tgcccgccct gcccgtcgtc
     1501 gatgctcccg cgcgagccga cgcacgcacc gcgctggcga ccgcgcgtga ggcggcccgc
     1561 ggccgctcga cgttcgccga cgccggcccc ggccagctga gcgcgctcga gcgatcgctg
     1621 atcttcaccg atggaccgtc gcccgccccg ggcatcgtga gcgtcgccgc atccggtggc
     1681 cgatgggccg gcgcggtgat gggcacgtcg ggcacctgtt actgggtccg cctgggtcac
     1741 accggcacca cgttcggctc gggcgagctg tgcaccgggt tcgccgccct gtcggccgac
     1801 gacgccgcgt ggtgacctga gccgccgacg tcgctgcggc acatcggttc gcgcgttccc
     1861 cacaccggga ctagactccc gcctcatccg cacacccgcg ctcttcgtga aggagggttc
     1921 ctgtcgtgac gcatgtctcc gtgaccgtga acggggagca acgcgaggcc gacgtcgagg
     1981 cccgcacgct cttggtctac ttcctccgag agaccctcgg gctcaccggc accaacgtgg
     2041 gatgtgacac gtccacatgc ggttcatgca cggtgatggt ggacggtgag tccgtgaagt
     2101 cctgcacgat gttgaccgcc caggccgatg gccgtgcggt caccacgatc gaggcgatgg
     2161 ccgacggcga cacgctgcac ccgctgcagg aggcgttcca ccgcaaccac ggtctgcagt
     2221 gtgggtattg cacaccaggc atgatcatgg cggcggcgag ctatctgaag gagaaccccg
     2281 cacccaccga ggaggaggtc cgcgagtcgc tcgagggcaa cctctgtcga tgcacgggtt
     2341 atcagaacat cgtcaagtcg atcctggatg ccgccggttc catgagcggc gcaaaggcct
     2401 gaggggggag cggagatggt cgcaaccgag acgttcatcg gccagccggt cctgcgcaag
     2461 gaagacggcg acctgctgac cggacaggca cggttcatcg acaacttcac gatgccgggc
     2521 atgctgtgga tggcgatggt tcgcccgccc tacgtgcacg ccacgatcaa ctcggtcgac
     2581 acgagcgcgg cggcttcgat gccgggcgtc gtcggcgtct acaccggcaa ggacctgagc
     2641 ctgggcgcgc tgccgttcgt gtggcccatc accgaggaca tcaagatccc ggtgcactac
     2701 ccgctcgcca ccgacaagat ccggttcgcc ggcgacgccg tggcggtggt gttggccgag
     2761 acacgagagc aggcagagga cgccgccgag atggtctcga tcgaggcctc cgagctcaag
     2821 gcggtcaccg acctccgcga agccgccaag gacgaggtga tcatccacga cgacctcggc
     2881 accaacgtgg tcgtgcactg gagccacggc ggcgcgggcg accaggacat cttcgagtcg
     2941 gcgccggtga tcgtgcagga gacctacgac cagccacgac tgatcccgaa cgcgatggag
     3001 cctcgtgggt gcctcgccta cggcgtgggg gcgatggacg agtggacctt ggtgagcgcc
     3061 acccagatcc cgcacatcgc gaaggtgacg ctggcgggcg tggtgggcat cgaggagccg
     3121 aagttccgcg tgatcgcccc cgacgtcggt ggcgggttcg gttccaagct caatgtctat
     3181 gccgaggaag cgctcgcgtg tgccctggcc aagcggtcgg gtcgcccggt gaagtggatc
     3241 gagggccgcc aggagaacta cgtcgccacg atccacggcc gcggcgtgac gcacgactgc
     3301 acgctggccg gtaccgaaga cggcttgatc ctcggcgtca agttcgtcga gctcgccgac
     3361 atgggggcct acttccaact gctcacgccc gggatccccg agctgggcgc ctgggtctac
     3421 atggggccat acaggcccga gacgtactgg tatgagttcc ggggcatcgt cacgaactgc
     3481 acgcccaccg acgcctatcg cggcgccggc cggcccgagg ccacctacgt cctggagcgt
     3541 ctcgtcgacg cgttcgccag gaagatcggc aaggatcccg cggacgtgcg gcgcatgaac
     3601 ttccacccgc cgttcgacga ggcgaccacc tcgatctgcg gcctcaacgt cgacagtggc
     3661 aactacgagc cgctgctcga caaggcactg gaactgagcg cgtacgacga ggtccgcaag
     3721 gagcagcagg ctcgccgcga ccgcggtgac acgaagcagc tcggcatcgg tctcagcacc
     3781 tacatcgaga tgtgcggcct ggcgccctcg aacatccttg gcgcactgcg ctatgccgcg
     3841 ggcggatggg atggcgccac gatcgaatgc acacccggcg gcggtgtcat cgtgaagacc
     3901 ggcacgtcgc cgcacggaca ggggcacgag accagctggt cgcagatcgt ggccgacggg
     3961 ctcggcgtcg acccggacaa ggtcgaggtg ctgcacggcg acacggcgat cacgccgctg
     4021 ggcatggaca cctacggctc ccgttcgctg tcggtcgggg gcgtggcgct gcacttcgcg
     4081 atggagaaga tcaaggagaa ggcacgcacg atcgctgcgc acgaactcga ggtgagcgag
     4141 gacgacctcg aatgggccga cggttcctac cgggtgaagg gcgcgcccga caaggcgcgc
     4201 accatccccg acctcgcggt gagtgcgtgg cacgcccacg acctgccggc cggggtggag
     4261 ccccacctca acgccacggc cgtctatgac ccgcccaact tcacctggcc gtctggggcg
     4321 cacatcgcgg tggtcgaggt cgacaccgag accggcgcca ccgacatcat caagtacatc
     4381 gcggtcgacg actgcggcac ggtgatcaac ccgatgatcg tggagggtca ggtgcacggc
     4441 ggcgttgccc aaggcgtggc cgaggcgctc tacgaggaag cgatctacga cgaaggtggc
     4501 aacctgacca cgtcgtcgat gacccagtac ctggtgccca cggcggtgga gatccccgcg
     4561 atcatcaccg acaacagcca ggagacaccc tccaccacca acccgctggg tgtgaagggg
     4621 atcggcgagg ccggcacgat cgcctcgccg cccgcggtga tcaacgcggt ggtggacgcg
     4681 ctctcccacc tcggcgtgac cgaggtgggc aagccggcgt cgcccgagcg ggtctggcga
     4741 gctatccagg acgcgcgagg aggtgcggca tgatcccggc tcccttcgag tacgcccgcg
     4801 ccgagtccgt cgaccacgcg atcgagctgc tgggctcgag cgaggacgcg aagatcctcg
     4861 cgggtggcca ctcgctgctg ccgttgatga aggtgcggtt cgcccagccc tccttgctgg
     4921 tcgacatcgg ccgcatcggc gacctgtcgt acgtgcgcga ggacgacgac gtcatcgcga
     4981 tcggcgcgct cacccgccac caagatgtgg cgaacagcga gctgctgcag gcgtcgtgcc
     5041 cgatcgtggc ggatgccgcc agccagatcg gtgacccaca ggtccggcac atgggcacga
     5101 tcggcggcgc ggtcgcccac tcggacccgg cctcggacat gcccacggtg atgctcgcgc
     5161 tcggcgcaca gatggtggtc cgcagcgccg gcgggcagtc gaggacggtg gcggccgccg
     5221 acttcttcca ggggttgt
//
LOCUS       DATDBZ010000484         3538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1173097, whole genome shotgun sequence.
ACCESSION   DATDBZ010000484 DATDBZ010000000
VERSION     DATDBZ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173097"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="VIX62_11345"
     CDS             complement(<1..135)
                     /locus_tag="VIX62_11345"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY5860872.1"
                     /translation="MNLLLSIPSPPSGTIHIGPLQLRAYGLMIALGVLAAVWLFGRRL
                     E"
     gene            complement(132..887)
                     /locus_tag="VIX62_11350"
     CDS             complement(132..887)
                     /locus_tag="VIX62_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5860873.1"
                     /translation="MTTTLLPGAEPWSHAAGPAGALCLHGFTGSPNSMRGVAQAYAAA
                     GFSVELPRLPGHGTTVEEMMTTGWLDWTGEAEAAYQRLAARTESVVVAGLSMGGSLTL
                     WTAAQHPEVAGIVCINPATQPQPAEVMDMVHGMIDEGTELMPGIGSDIAAPGVVETAY
                     LGTPLRPLVSMIAGIVDLVPSYASIHCPLLLITSTQDHVVDPVQSDYLAERYGGPVER
                     IVLERSYHVATLDYDRELIEEQAVEFGRKVTTR"
     gene            complement(897..1217)
                     /locus_tag="VIX62_11355"
     CDS             complement(897..1217)
                     /locus_tag="VIX62_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018830107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY5860874.1"
                     /translation="MAGTARRERIELRTRNPKWKNPPVRIEMLECINCDACLRHCPPH
                     FGAIFNHGPDVVIIPELCSGCDKCLPACPVNCIYPFPDWEQRGSPADWWDEPGSDDDP
                     YRTR"
     gene            1280..1897
                     /locus_tag="VIX62_11360"
     CDS             1280..1897
                     /locus_tag="VIX62_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5860875.1"
                     /translation="MSTTRRLTDQGKERKQQLLDAAATLFASRGYGPTRIADICEAAG
                     VAKGLFYWYFETKESLFAELVRSMRTQLRRSQGAAMDPDADAVTRIRQGAEASVQFMA
                     EHMAFFALLEMERTDEAILPVLREGSEVYVADTSRLVAEAQGAGLVPDDHDARLLAMG
                     VHGAVSHFSHFHRSGRIDLSVDELAVFVGEWVVRALTAESARMPR"
     gene            1894..2760
                     /locus_tag="VIX62_11365"
     CDS             1894..2760
                     /locus_tag="VIX62_11365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY5860876.1"
                     /translation="MTISVGIAFANSGPFAQPEAAAALARTAEEHGVESLWTVEHVIV
                     PVGYTSTYPYHPKGRMLGEELDIPDPLIWLAYVAGQTSSIKLGTGILILPQRNPIVTA
                     KEIATLDLLSGGRVQLGVGVGWLEEEFDALGVPFAERGRRTDEHIEVMRALWSGRASF
                     DGEFTRFGEAIAHPVPAQGQGVPIVIGGHTPIAARRAGRLGDGFFPAKGSVDELGVLV
                     EVMRETAAAAGRNPDEIELTTGARDLDGMKRLHDIGFTRFVAPPPAYDIAQLPAAFAR
                     LQEELVGPLASI"
     gene            2842..>3538
                     /locus_tag="VIX62_11370"
     CDS             2842..>3538
                     /locus_tag="VIX62_11370"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5860877.1"
                     /translation="MARRPPSRHLESSALTAVLPAIALVPVCALALALVWLVVRLWWD
                     VPYWLFVLAYLGSGVLLFVRPIQRLVLTRLLGARRPTRDELAVLEPVWRSVTQAVHVP
                     PRRFVLAVLDADELNAFASGGHLVVVTSYAIEVLPDDELAGVLAHELSHHLGLHTIAL
                     TVAQWLSLPIVLLARIGFSLQNIARAATDTFARGSSSFTIVGRLISGILNAAAWVFLV
                     AILVGNAIGNVVGR"
BASE COUNT          533 a         1190 c         1245 g          570 t
ORIGIN      
        1 ctccagccgg cgcccgaaca gccagacggc cgccagcacg ccgagggcga tcatcagccc
       61 gtacgcccgc aattgcagcg gcccgatgtg gatcgttccc gacggggggc tcgggatcga
      121 caagaggagg ttcaccgagt cgtgaccttg cggccgaact cgaccgcctg ctcctcgatc
      181 agctcacggt cgtagtcgag cgtggcgacg tggtagctcc gctcgagcac gatccgctcg
      241 accggtccgc cataccgctc cgccaggtaa tcggactgca ccgggtcgac gacgtgatcc
      301 tgggtggacg tgatcagcag cagtgggcag tggatcgatg cgtagctcgg cacgaggtcg
      361 acgatgccgg cgatcatcga gaccagcggg cgcaacggcg taccgaggta ggccgtctcg
      421 acgacgccgg gcgcggcgat gtcggacccg atgcccggca tcagctcggt gccctcgtcg
      481 atcatgccgt ggaccatgtc catcacttcg gccggctgtg gctgggtggc ggggttgatg
      541 cagacgatcc ctgctacctc ggggtgctgc gcggcggtcc acagcgtcag cgagccgccc
      601 atcgacaggc cggccacgac gaccgactcg gtccgggccg ccagccgctg gtaggccgcc
      661 tccgcctcgc cggtccaatc gagccatccg gtggtcatca tctcctcgac cgtggtgccg
      721 tgaccgggga ggcgaggcag ttcgaccgag aaaccggctg ccgcgtacgc ctgcgcgaca
      781 ccacgcatcg agttcggact accggtgaaa ccgtgcaagc acagggcgcc ggcgggtccg
      841 gcggcgtggg accagggctc ggcgcccggc aagagagtcg tcgtcatggt gcctgtctag
      901 cgcgtgcgat acggatcgtc gtcgctgccc ggttcatccc accagtcggc gggcgagccc
      961 cgctgctccc agtccgggaa cgggtagatg cagttgaccg ggcacgccgg caggcacttg
     1021 tcgcatccgc tgcagagctc ggggatgatg accacgtcgg gaccgtggtt gaagatggcg
     1081 ccgaagtgcg gggggcagtg gcggaggcag gcatcgcagt tgatgcactc gagcatctcg
     1141 atgcgcacgg gcgggttctt ccacttcggg ttgcgcgttc gcaattcgat ccgttcgcga
     1201 cgcgcagtcc cggccatgcg gggagcatac gagacgaggg gagattgacc cgtgggtcaa
     1261 ggtgcggtgg actgtgcgtg tgagcacgac gcggcggttg accgatcagg gcaaagagcg
     1321 caagcagcaa ctgctggacg ccgctgcgac cctgttcgcg agccgcggct acggaccgac
     1381 ccggatcgcc gacatctgcg aggcggccgg cgtggcgaag ggcctcttct actggtactt
     1441 cgagaccaag gagagcctgt tcgccgagct ggtgcggagc atgcgaaccc agctgcgccg
     1501 gtcccaaggg gcagcgatgg atccggacgc tgatgcggtg acgcgcatcc gccagggggc
     1561 agaggcgagc gtccagttca tggccgagca catggccttc ttcgctctcc tggagatgga
     1621 gcggaccgac gaggcgatcc tccccgttct gcgcgaaggc agcgaggtct acgtcgccga
     1681 cacgagccgg ctcgttgcgg aggcgcaagg cgccgggctc gttccggacg accacgacgc
     1741 ccgactgctg gcgatgggcg tgcacggtgc ggtgtcgcac ttcagccact tccaccgctc
     1801 cggtcggatc gacctgtcgg tcgacgagct ggcggtgttc gtgggcgaat gggtggtccg
     1861 cgcgctcacg gccgagagcg cgaggatgcc gcgatgacca tctccgtcgg aatcgcgttc
     1921 gccaactcgg gcccgttcgc ccagccggag gcagccgccg cgctcgcccg cacggcggag
     1981 gaacacggcg tcgagtcgct gtggacggtc gagcacgtga tcgtcccggt cggctacaca
     2041 tcgacctacc cgtaccaccc gaagggccgc atgctgggcg aggagctcga catcccggat
     2101 ccgctgatct ggctcgctta cgtcgccggg cagacgtcga gcatcaagct cggcaccggg
     2161 atcctcatcc tcccccagcg caacccgatc gtgacggcga aggagatcgc gacgctcgat
     2221 ctgctctccg gcgggcgcgt gcagctcggc gtcggggtcg gctggctgga ggaggagttc
     2281 gacgcgctgg gcgtgccctt cgccgaacgc ggccgccgca ctgacgagca catcgaggtg
     2341 atgcgggcgc tctggtcggg tcgcgccagc ttcgacggcg agttcacgcg gttcggcgag
     2401 gcgatcgccc atccggtgcc ggctcagggt cagggcgtgc cgatcgtgat cggcgggcac
     2461 acgccgatcg cggcccgccg ggcaggacgg ctcggcgacg gtttcttccc tgcgaaggga
     2521 tcggtcgacg agctcggcgt cctcgtggag gtcatgcggg agacggcagc ggcggctggc
     2581 cgcaaccccg acgaaatcga gctcacgacg ggagctcgtg acctcgacgg tatgaagcgg
     2641 ctgcacgaca tcgggttcac gcggttcgtc gcaccgcctc ccgcctacga catcgcacag
     2701 ctgccggcgg cgttcgcgcg gttgcaggag gaactcgtcg ggccacttgc gtcgatctga
     2761 caggattcga ctcaggatcg tcggctgcga gaccgtatcg tcagtggcga cgtgacgagt
     2821 tgagaggaac accaggccga catggcgcgt cgaccaccga gcagacatct ggagtcgagc
     2881 gccctcacgg cagtactgcc ggccatcgcg ctcgtgccgg tctgcgcgct cgccctcgcg
     2941 ctcgtctggc tcgtcgtccg gctctggtgg gacgtgccct actggctgtt cgtcctcgcc
     3001 tacctcggct ctggggtcct gctgttcgtg cgaccgatcc agcgcctggt gctcacccgg
     3061 ttgctcggtg cccggcggcc gacccgcgac gagctcgccg tcctcgaacc ggtgtggcga
     3121 tcagtcaccc aggccgtcca cgtcccgccc cgacggttcg tgctcgccgt gctcgacgcg
     3181 gacgagttga acgcgttcgc gagcggcggt cacctcgtgg tggtcacgtc ctacgcgatc
     3241 gaggtgctac cggacgatga gctcgccggt gtgctcgccc acgagctcag tcatcacctc
     3301 ggtctgcaca cgatcgcgct gacggtggcg cagtggctgt cgctgccgat cgtgctcctc
     3361 gcccgcatcg gcttctcgct gcagaacatc gcccgggctg cgaccgacac gttcgctcgc
     3421 gggtcgtcga gcttcaccat cgtcgggcgc ctcatcagcg gcatcctcaa cgccgcggca
     3481 tgggtcttcc tcgtcgccat cctggtcggc aacgcgatcg ggaacgtcgt cggccgcg
//
LOCUS       DATDBZ010000485         2118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1383396, whole genome shotgun sequence.
ACCESSION   DATDBZ010000485 DATDBZ010000000
VERSION     DATDBZ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383396"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /gene="acs"
                     /locus_tag="VIX62_11375"
     CDS             <1..1794
                     /gene="acs"
                     /locus_tag="VIX62_11375"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY5860878.1"
                     /translation="FSHWDSVMDWEPPWVRWFSGGTLNASYNCLDRHVEAGGGDKVAY
                     HWEGEPGDTRTITYADLYEDVCRLANGLKSLGVAKGDRVNIYLGMVPELPIAMLACAR
                     IGAAHSVVFGGFSAEALRDRINDAEAKVLITADGAWRRGTVVPLKDNADAAVAQCPSI
                     EHVVTVRRCENEHAFQAGRDIWYHELVADQPPECEPEPMDAEDLLYLLYTSGTTGKPK
                     GIAHTTGGYMTQVAATHRMIFDIHEDDVYWCAADCGWVTGHSYIVYGPLANHTTGVMY
                     EGAPDWPDKDRWWDIIERYGVTILYTAPTAVRAFMRWGTEHADKHDLTSLRLLGSVGE
                     PINPEAWVWYWKVIGGERCPIVDTWWQTETGAILISPMPGLTLLKPGSATFPFPGIAA
                     DVVDERGVSVDLGGGGYLVLRHPWPAIARTIWGDPDRYVETYFGRYGHDVYVAGDGAR
                     LDAEGFYWLLGRIDDVMNVAGHRLSTFEIESALVDDEKVAEAAVVSRPHEVKGEGIVA
                     FVTLKSGYDGDEAMLNELREHVAKVIGPIAKPDVIIFTPDLPKTRSGKIMRRLLRDVA
                     RGDQLGDVTTLANAEIVESIREQAAAGAGEE"
     gene            1802..>2118
                     /locus_tag="VIX62_11380"
     CDS             1802..>2118
                     /locus_tag="VIX62_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5860879.1"
                     /translation="MTIDEKIFLPQEAEFVAVQRDCALGTLRPDGRPHVVPISTVLDL
                     DRLVFATERDTQKVRNIEGNANVAICYDEYHEDWSQLVQVIAEGEAYILDGGFEFERD
                     RTLL"
BASE COUNT          371 a          684 c          728 g          335 t
ORIGIN      
        1 ttctcccact gggattcggt catggactgg gagccgccgt gggtgcggtg gttctcgggg
       61 ggcacgctca acgcttccta caactgcctg gaccgtcatg tggaggccgg tggtggcgac
      121 aaggtcgcct accactggga gggcgaaccc ggtgataccc gcacgatcac ctacgccgac
      181 ctctacgagg acgtgtgcag gctcgccaac gggctgaagt cgctcggggt tgccaagggg
      241 gaccgggtca acatctacct gggcatggtg cccgagctcc ccatcgcgat gctggcctgt
      301 gcgcggatcg gggccgcgca ctcggtggtc ttcggaggct tcagcgccga ggcgctgcgc
      361 gaccgcatca acgacgccga ggccaaggtg ctgatcacgg ccgatggtgc gtggcgacgc
      421 ggcaccgtcg tcccgctgaa ggacaacgcc gatgcagccg tggcgcagtg tccctcgatc
      481 gaacacgtgg tcaccgtgcg tcggtgcgag aacgagcacg cgttccaggc gggccgagac
      541 atctggtacc acgagttggt ggccgatcag ccgcccgagt gtgaacccga acccatggac
      601 gccgaggatc tgctctacct gctgtacacc agcggcacca ccggcaagcc caagggcatc
      661 gcccacacca ccggcggcta catgacacag gtggccgcca cccaccggat gatcttcgac
      721 atccacgaag acgacgtgta ctggtgtgcg gccgactgcg ggtgggtgac cgggcacagc
      781 tacatcgtct atggaccgct cgcgaaccac accaccggcg tgatgtacga gggcgcaccc
      841 gactggcccg acaaggaccg atggtgggac atcatcgagc ggtacggggt cacgatcctg
      901 tacacggcgc ccaccgcggt gcgtgcgttc atgcgatggg ggaccgagca cgccgacaag
      961 cacgacctca cctcgctccg gctgctgggt tcggtgggcg agccgatcaa cccggaggcg
     1021 tgggtttggt actggaaggt gatcggcggg gaacggtgtc ccatcgtcga cacctggtgg
     1081 cagacggaaa ccggcgcgat cctcatctcg ccgatgccgg gcctcacgct gctgaagcct
     1141 gggtcggcga cgttcccgtt cccggggatc gctgccgacg tcgtcgacga acggggcgtg
     1201 agcgtcgacc tgggcggcgg tggctacctg gtgctgcgtc acccgtggcc cgcgatcgcc
     1261 cgcacgatct ggggtgatcc cgatcgctac gtcgagacct acttcggtcg ctacggccac
     1321 gacgtctacg tggccggcga cggcgcccgc ctggacgccg aggggttcta ctggctgctg
     1381 ggcaggatcg acgacgtgat gaacgtcgcc ggtcaccgtc tgtcgacctt cgagatcgaa
     1441 tcggcgctcg tcgacgacga gaaggtggcc gaggcggcgg tggtctcgcg tccgcacgag
     1501 gtcaagggag aagggatcgt ggccttcgtc accttgaagt cgggctacga cggtgatgaa
     1561 gcgatgctca acgagctccg cgagcacgtg gcgaaggtga tcggcccgat cgccaagccc
     1621 gacgtgatca tcttcacgcc cgacctgccg aagacccgct cgggcaagat catgcgccgg
     1681 ctcctgcgtg acgtcgctcg tggcgaccag ctgggcgacg tcacgacgct cgcgaacgcc
     1741 gagatcgtcg agtcgatccg ggaacaagcc gccgccggcg cgggcgagga gtgacccatc
     1801 catgacgatc gacgagaaga tcttcctgcc gcaggaagct gagttcgttg cggtgcagcg
     1861 agactgtgcg ctcggcacgc tgcgacccga tgggcgtccg cacgtggtgc cgatctcgac
     1921 ggtgctcgac ctggaccgcc tggtgttcgc caccgaacgc gacacccaga aggttcgcaa
     1981 catcgagggc aacgccaacg tcgcgatctg ctacgacgaa tatcacgaag actggtcaca
     2041 gctggtgcag gtgatcgccg agggcgaggc ctacatcctc gacggcggct tcgagttcga
     2101 acgcgaccgc acgctgct
//
LOCUS       DATDBZ010000486         9237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1383650, whole genome shotgun sequence.
ACCESSION   DATDBZ010000486 DATDBZ010000000
VERSION     DATDBZ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383650"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1027)
                     /locus_tag="VIX62_11385"
     CDS             complement(272..1027)
                     /locus_tag="VIX62_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860880.1"
                     /translation="MRAHPGPVVAVAVLVMVLTACEGGDDGSSAAPEPQSATTTSPTP
                     PLIPLEDGPRLEPGTYSVSSEGRTGSDPLLWSIVDYTITIPEGWKGHTGHYLSKYENV
                     DETRGLVIYPVLIDEIYADPCEGERGPIAAVGPEVNDLVDALLAQPGTVATKPVRTTI
                     GGLPATRIDLEVPKGADLRSCFLADFGAPGLQVWFSKPTQKYFVLMPGSTASVYVVDV
                     DGERQVFLTQHGPAASDEDLRELQSMLDSIHID"
     gene            complement(1362..1574)
                     /locus_tag="VIX62_11390"
     CDS             complement(1362..1574)
                     /locus_tag="VIX62_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860881.1"
                     /translation="MSRDVPDPPDGLSARSVAAWPALIADVVAAAGGADVDLELAGEL
                     LRVRDRLDEVRVVLAVEGLVTEGSTG"
     gene            1675..1812
                     /locus_tag="VIX62_11395"
     CDS             1675..1812
                     /locus_tag="VIX62_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860882.1"
                     /translation="MHVWGTTKHGGRRVGETACDRFALQNYRRRLVTIEDAETSLNGA
                     R"
     gene            2686..3225
                     /locus_tag="VIX62_11400"
     CDS             2686..3225
                     /locus_tag="VIX62_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5860883.1"
                     /translation="MHPWSMQAEGEVVHLPTEGAEHGAPFVTFVEEEHVRLFKALYFV
                     TGSREEAEDLAQEAFLKLWERWDRIDTIEDPTAYLFRVALNGFRMRRRRTTMAIRRQP
                     APPEQRDGYVEAEMREDVRRMLLEVTPRQRAALLLVDLLGYPAEQAASILRIRPSTVR
                     NLLSQGRKTLRSTEGVRDA"
     gene            3218..4255
                     /locus_tag="VIX62_11405"
     CDS             3218..4255
                     /locus_tag="VIX62_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860884.1"
                     /translation="MPDLREVFQMSTQKVRPDRGFTERQEFRQRRKVRNQKIGAYVVV
                     AAVAAVAVIAFAAVQGGRGDTTVPGSSAPPSAAVPAAATDSYLDIATGERSPVAADLS
                     GARALEVSPNGQAVVYNTCCNNDAVHVANLDGSGAEIITPEKLDGYGATWIDDETILF
                     QGRPEGTSEMGALYVADLSAGDLTKVTDLPDGQKAAWIVISDVSPDGTTVLFHLPRWA
                     GEQERWDLWTAPLAGGEPTLLRRNAGSPQYTPDGSIVFLGHAVPFHGDGIWIMDGDGS
                     DARPIVENPGDTFTWPQVSPDGTTVAYGHDGETEIIAIDGCCGTGGSGEAIGEFRHEV
                     AWYGNDTLIVD"
     gene            complement(4319..4987)
                     /locus_tag="VIX62_11410"
     CDS             complement(4319..4987)
                     /locus_tag="VIX62_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5860885.1"
                     /translation="MVAEPRSPRNAKTQVKRDRIVETAMRQFAEHGFQGAKVEDIAIE
                     LGIAKGSIFQHFGSKAGLFLQAYKRAVLTLPAWLDAPEEVRAQGFFATVRFWLERTEH
                     LIKEDWIPNRVVLIGNYGTDLTLKRDINRWLVSEDPYGTLEFVEWGQERGEVRADVDL
                     EIIVSMVDWLSNSLQDALVTEELDPGLFHRLAGQPERQRMRVEHFTVLLESAIGPRRD
                     PGPA"
     gene            5216..5869
                     /locus_tag="VIX62_11415"
     CDS             5216..5869
                     /locus_tag="VIX62_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY5860886.1"
                     /translation="MHEDLDVLKRALIAADMDLGVAAAQRLVDSGVDPKWILEEGMAV
                     AMFDLGEMWKRGEVFLPEVVASAEVFKRCNAIVEPVLLAQREEGDEANAIVVLATVKG
                     DLHDLGKNMVGAMLRTSGFDVHDIGKDVSADRIVEVVRELHPQIVGLSALLTTTVPYQ
                     QRVIEKLEAEGLRNQVKVMVGGAPVTPEWAEKIGADGYANNAPEAVEIAKKLVGVAQ"
     gene            5866..6765
                     /locus_tag="VIX62_11420"
     CDS             5866..6765
                     /locus_tag="VIX62_11420"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5860887.1"
                     /translation="MSGVATTILSGPGGEVAIGDEHPFRIIGERINPTGRTQLTEELK
                     VMDMATVQRDALAQVEAGAAVLDLNAGIPGFDESAMLSAMVKTVHEVVGVPLCIDSST
                     PEALEAAVPLAQGKVLINSVTAEDHSLERLLPLVARFGAAVIGMANDSDGISMDPQVR
                     LAAARKIVERAADHGIGHEDVIIDPLTMPIGAAPDAATSMYETVKLLRSELRVNVSCG
                     ASNISFGMPDRRGIDSAFLTMSIVTGMNTAITNPLHIETRKAILAADLLLGRDEYGAN
                     WIALHRAELAAANAANAGGEPTA"
     gene            6762..8678
                     /locus_tag="VIX62_11425"
     CDS             6762..8678
                     /locus_tag="VIX62_11425"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HEY5860888.1"
                     /translation="MTKKVEVTYQPSGKTVRVPEGTTLFNAAHWAGLPIESTCGGRGT
                     CGKCGVKVLSGHVERSLADYRHLNDQLDEGWRLSCQAEITEDTECDVPRLMKMPKAAT
                     MGVGRFVLLEPNVVKLFLELPPPSLEDHRSHLARVLDAVDEAGYNRNYDWEVMPRIAQ
                     AFRATSNVTATLVGEYLVDVEPGDTTDRMFGASFDIGTTTCVCTLVDLRNGATVGVAS
                     TINHQAPFGADVIARMAKAMHGEENVVQLREAAISTVNELLGRAREEAGVAQHEVYEA
                     VVAGNATMMHLLCGVNPESLALAPYVATFTEPQDLRGDQARFDMHPLGWVALFPSIGA
                     YVGADIVADIVATGLVRDSETRLLVDVGTNGEIACGNEERSVATAAPAGPAFEGGEIV
                     HGMRATDGAIEGVTLTNGEVELQVIGGEDAEPRGICGSGLIDIVAQLRLAGLLDDGGK
                     MMSREEAEDAGHSLADRLVMREDVKAFHLHGDVVLTQQDVRALQFAKGAISTGIETAM
                     RALGLEPSDLDAVMLAGSFGSYINPQSARVIGLVPPVPVERIKAVGNTASEGAKMALM
                     SFREREVAWEIPGIVEYIELSGEEDFNDRFIGNLGFPPIEDLHAAQAANEAAIVARAE
                     ARASASVGSEDTSP"
     gene            8675..>9237
                     /locus_tag="VIX62_11430"
     CDS             8675..>9237
                     /locus_tag="VIX62_11430"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HEY5860889.1"
                     /translation="MTAPGARVALLACGAVAIDTKTLVERHGWNADVHGISSDLHMTP
                     LEIAPAVEEKLIALLPRYERVIVVYGDCGTGGRLDEVLSRYPAVRPAGVHCFQWYSGE
                     LYRRFETDIGIYFLTDWLVTNWDRAVIKGLGLDRFPWLRETYFGNLSRVLFVRQHPDP
                     EREAKAREIAEYMQLPLEIHDLGIEPL"
BASE COUNT         1504 a         3073 c         3232 g         1428 t
ORIGIN      
        1 ccctgccggt cgttgggggc tggcccgcta cagccggggg ggcgagggct gggggtccgt
       61 gggcacaggg gcgcctacgg cgtccgattc cgcagggagt gcttgtcgat gcgcgagggc
      121 ctacgatccc ctgcatggac gaccaccggg gacccacctt cttcgactgg atcaccgacc
      181 tgttggcgtt cgggatgatc ttcgccctat tactgatcgt ggtgatgcac tcgctcggct
      241 ggtgggccta gttccaaccc ggcgcggtgg ctcaatcgat gtggatggag tccagcatcg
      301 actggagctc ccggaggtcc tcgtcggaag ctgctggccc gtgctgcgtc aggaacacct
      361 ggcgctcgcc gtccacgtcc acgacgtaga cactggcggt gctgccgggc atgaggacga
      421 agtacttctg ggtgggcttg ctgaaccaga cctgaaggcc cggggccccg aagtcggcca
      481 aaaagcagga ccggaggtcg gcgcccttcg ggacctcgag atcgatccgc gtcgccggaa
      541 ggcccccgat ggtcgtccgc accggtttgg tggcgacggt tcctggttgc gcgagcaggg
      601 cgtcgacgag gtcgttgacc tccggcccta ctgctgcgat cgggccacgt tccccctcgc
      661 acggatcggc atagatctcg tcgatcagca ccgggtagat gacgagccca cgggtctcgt
      721 cgacgttctc gtacttggac aggtagtggc cggtgtggcc cttccacccc tcggggatcg
      781 tgatcgtgta gtccacgatc gaccacagca gcggatccga tcctgtacgc ccttcgctcg
      841 agaccgaata ggtgccgggc tcgaggcgcg ggccgtcctc cagcggtatc aacgggggcg
      901 tgggagaggt ggtcgtcgcc gactgcggct cgggtgcagc cgacgagccg tcgtccccgc
      961 cctcgcacgc cgtcaagacc atcaccagca cggccacggc taccacaggt cctgggtgag
     1021 ctctcatgtc gcttctcctc tcaggctctt cggcgtccgc cgaagggatg gtccggaacg
     1081 gtgttcgctt cagccggagt cccacgtatt cgtcctgagc gtgaaggcca cgcatataga
     1141 tgccacggag ggccgaatcg cctagagagg tgacagaaac ccgcgccagc gcgatgctcg
     1201 agcgatgtca gggacggcta cttccctcgc tcgagccgcc cgtccttgcc cacgcgaacc
     1261 gaccagccac gcttcgacgg atcgagcagg aggcgatcgg tgatcccgag cagctgggat
     1321 cgcagcgact cctcgaccgc gacgagaggg tgcgcccggc gttagccggt gctgccctcg
     1381 gtgacgagac cctccacggc aagaaccaca cgcacctcgt cgagccgatc gcgcaccctc
     1441 agcagctcgc cggccagctc gaggtcgacg tcggcgccac cagctgcagc gacgacatcg
     1501 gcgatgagcg cgggccacgc ggcgacgctg cgagcgctca ggccgtcggg cggatcgggc
     1561 acgtcacggc tcacccggct cgccccggaa aaatccggcc cagcacttcc tcgcgagatc
     1621 cggcatggtg cacgtgggat gaccagcttg ccatctgcct gcaagcacga gtggatgcac
     1681 gtttggggga ccacgaagca cggtgggcgc cgggtggggg agacggcgtg cgatcggttc
     1741 gcgctgcaga actaccggcg ccggctggtg acgatcgagg acgccgagac gtctctcaac
     1801 ggagcccgct gaatcgcttg aagtccaagg gctaggcttc cgtgacatgg cggcgacgct
     1861 cggccgtgcc tctttcctcg cgacgctgct ggtcgtcgtt ctctctacgt cgtgcactgt
     1921 cacctcggtt ccgcgggctc atccggcgtc acgggtcgag acagtgacga tcggcgacct
     1981 tcaggaggca ctcgcccgct ggcatcgcgt atcggccacc gtcatctatc gcacgaaccg
     2041 acagcagccc ggtcggccgc cgtcggccca ccagtgtctt cgccgctacg tcgacgatcg
     2101 tgccgacatc ccaatcggac tggcgaaatg cgatcctgcg ggggtcgcgc gattggtgtg
     2161 ggatccacct gctcgatggc ggctcgaggt caccgaggcg taccggatca ccagcgcgac
     2221 ggtcgtgggc gatcgctcgg tgttgtgcgt gcaggcgaac gggagagacg aacggtgccg
     2281 tctgtggcgg agtagcgatc ggcccgtcca ggccctcccc ttccacgagc tggtcgccgg
     2341 cgttggctcg gtcctgcacg agctcggtat cgatcccttg gggcccgtga ccgtcacgca
     2401 ccgcctcgtc gggaatgccg cggccgactg tttcgaacgg gcctcggatg ggtcgtcggc
     2461 ggagtggtgc ttcgggagtg gtggaacgct cctcgcgctg gagctacgtg ccgaggatcg
     2521 agcgccgaca ttcgtcgaag tcaagcgctt ctcggccgac gtccgatggc ctcggcgttc
     2581 gacacgatga tctcctaggc aggcgtcccg gccttcttcc attgccacga aggatgttcg
     2641 ccggtggcgt agggtcggtg ctcatctaga caccctgagg ccatcatgca tccatggtcc
     2701 atgcaagcag aaggtgaggt cgtacacctc cccaccgaag gtgccgaaca cggggccccg
     2761 ttcgtcacgt tcgtcgagga ggagcacgtc cggctattca aggccctcta cttcgtcacg
     2821 ggcagccgtg aggaagccga ggacctggcc caggaggcgt tcctgaagct ctgggagcgc
     2881 tgggaccgga tcgacacgat cgaggatccc accgcctacc tgttccgggt ggcgctcaac
     2941 gggttccgga tgcgacgccg tcgcacgacg atggcgatcc gccggcagcc cgcgccgccc
     3001 gagcagcgcg acggctacgt ggaggccgag atgcgcgagg acgtgcgacg gatgttgctg
     3061 gaggtcacgc cgcgacaacg ggccgccctg ctgcttgttg acctgctcgg ctatcccgcc
     3121 gagcaggcgg cgagcatcct acggatccga ccgtcgacgg tacggaacct gctgagccag
     3181 ggccgcaaga cgctgcgatc gacggaaggg gtgcgggatg cctgacctgc gagaggtgtt
     3241 ccagatgtcc acccagaagg ttcgacccga tcgaggattc accgagcgcc aggagttccg
     3301 gcagcgacgc aaggtgcgga accagaagat cggcgcgtac gtcgtggtcg cggcggtcgc
     3361 ggcggtcgcg gtgatcgcgt tcgcggcggt ccaaggcgga cggggcgaca ccaccgtccc
     3421 cggatcatcc gctccgccgt ccgcagcggt cccggccgcg gccaccgact cgtacctcga
     3481 catcgccacc ggcgagcgct cacccgtggc ggctgacctg agcggggcca gagcgctcga
     3541 ggtctcaccg aacgggcaag ccgtcgtgta caacacgtgc tgcaacaacg acgctgtcca
     3601 cgtggcgaac ctcgacggtt caggcgccga gatcatcacg cccgagaagc tcgacgggta
     3661 cggcgcgacg tggatcgatg acgagacgat cctgttccag ggtcggcccg aaggcacgag
     3721 cgagatgggt gccctctacg tcgcggacct ctcggcgggc gacctgacga aggtcaccga
     3781 cctgcctgac gggcagaagg cggcgtggat cgtgatctcc gacgtcagcc cggacgggac
     3841 cacggtgctc ttccacctcc cccgatgggc gggagagcag gagagatggg acctgtggac
     3901 cgcgcccctc gcaggcggcg agcccaccct cctccgcagg aacgccgggt ctccgcagta
     3961 cacgcccgac ggttcgatcg tgttcctcgg tcacgcggtc ccgttccacg gggacggcat
     4021 ctggatcatg gacggcgacg ggagcgacgc acgcccgatc gtggagaacc ctggcgacac
     4081 cttcacgtgg ccgcaggtct cccccgacgg cacgacggtt gcgtacgggc acgacggtga
     4141 gaccgagatc atcgccatcg acggctgctg tggcacgggt gggagcggcg aggcgatcgg
     4201 cgagttcagg catgaggtgg cgtggtacgg gaacgacacc ctgatcgtcg actgaaccac
     4261 gcctgacaca cgagggccgg ggcgacgagc cccggccttc ggccctgctc gagccgtgct
     4321 acgccggacc tgggtcgcgc cgcgggccga tcgcgctctc gagcagcacc gtgaagtgct
     4381 cgacgcgcat tcgctggcgc tcgggctgtc cggcgagccg atggaacaac cccgggtcga
     4441 gctcctcggt gaccagggcg tcctgcaagg agttcgagag ccagtcgacc atcgagacga
     4501 tgatctcgag gtcgacgtcg gcccgcacct cgccgcgttc ctgcccccac tcgacgaact
     4561 cgagcgtgcc gtaagggtcc tcgctcacga gccagcggtt tatgtcgcgc ttgagcgtga
     4621 ggtcggtgcc gtagttgccg atcagcacga cgcggttcgg gatccagtcc tccttgatca
     4681 gatgctcggt tcgctcgagc cagaagcgga ccgtggcgaa gaatccctgg gcccgcacct
     4741 cctcgggagc gtcgagccag gccgggagcg tgaggacagc ccgcttgtac gcctgcagga
     4801 agaggccggc cttggacccg aagtgttgga agatcgagcc cttcgcgatg ccgagctcga
     4861 tcgcgatgtc ctcgaccttg gcgccttgga agccgtgctc ggcgaactgg cgcatcgccg
     4921 tctcgacgat gcggtcgcgc ttgacctggg tcttggcgtt gcgtggcgat ctcggctcgg
     4981 cgaccatccg gctcctcgtg accgtgggcg agaccaggct acggagccgg gcgctccctg
     5041 actgactccg ggtcactcga tggggacccc tggggctccc aacggtggtc tctcgctggc
     5101 atcatcgggt gtcggtggtc ctcgcagcaa cgctgacttc aggtcatcgt gagggccgac
     5161 cgaacgggtc atgctcacgg cccaacccgg cgacaacgcg cggaaggatg cggcgatgca
     5221 cgaagacctc gacgtcttga aacgagccct catcgccgcc gacatggacc tcggcgtcgc
     5281 cgcggcacag cgcctcgtcg actccggcgt cgatccgaag tggatcctgg aggaaggcat
     5341 ggccgtggcc atgttcgacc tcggtgagat gtggaagcgg ggcgaggtgt tcctgcccga
     5401 ggtcgtcgcg agcgccgagg tattcaagcg ctgcaacgcg atcgtggagc ctgtcttgct
     5461 cgcccagcgt gaggaaggcg acgaggccaa cgcgatcgtc gtgctcgcca cggtcaaggg
     5521 cgacctgcac gatttgggga agaacatggt gggtgcgatg ctccgcacga gcggcttcga
     5581 cgtgcacgac atcggcaagg acgtgagcgc ggaccgcatc gtggaggtcg tgcgtgagct
     5641 gcatccccag atcgtcgggc tctcggcgct gctgaccacc accgtcccgt atcagcagcg
     5701 cgtcatcgag aagctggagg ccgaggggtt gaggaaccag gtgaaggtca tggtgggagg
     5761 cgcgcccgtc acgcccgaat gggcggagaa gatcggcgcc gacggctacg cgaacaatgc
     5821 tcctgaggcg gtcgagatcg cgaagaagct cgtgggggtt gcccaatgag cggtgtcgcg
     5881 acgacgatcc tgagcggtcc cggcggcgag gtcgcgatcg gcgacgagca cccgttccgc
     5941 atcatcggcg agcgcatcaa cccgacgggg cgcacgcagc tcaccgagga gctgaaggtc
     6001 atggacatgg ccaccgtgca gcgcgatgcg ctcgcgcagg tggaagcggg cgcagcggtg
     6061 ctcgatctga acgccggcat cccggggttc gacgagtcgg cgatgctctc cgcgatggtg
     6121 aagaccgtgc acgaggtcgt cggcgtgccg ctgtgcatcg actcctcgac ccccgaggcc
     6181 ctcgaggccg cggtgccgct ggcccagggc aaggtgctga tcaactccgt cacggccgag
     6241 gatcattcgc tcgagcggct gctgccgctg gtcgcgagat tcggcgccgc ggtgatcggc
     6301 atggctaacg actccgacgg catctcgatg gatccgcagg tgcgcctggc ggccgcgcgc
     6361 aagatcgtgg aacgcgccgc cgaccacggc atcggtcacg aggacgtgat catcgaccca
     6421 cttacgatgc cgatcggcgc tgcgcccgac gcggcgactt cgatgtacga aacggtgaag
     6481 ctgctccgct ccgagctccg cgtgaacgtg agctgcggcg cctcgaacat ctccttcggc
     6541 atgccggacc gccgtggcat cgactcggcg ttcctcacga tgtcgatcgt gaccggcatg
     6601 aacaccgcca tcacgaaccc gctgcacatc gagacgcgca aggcgatcct cgccgccgac
     6661 ctgctgctcg ggcgcgacga gtacggcgcg aactggatcg cgctgcaccg cgccgagctc
     6721 gctgcggcga acgcggcgaa cgccggcggc gagccgaccg catgaccaag aaggtcgagg
     6781 tcacgtacca gccgagcggg aagaccgtcc gggtgcccga gggcacgacg ctgttcaacg
     6841 cggcgcactg ggccgggctg ccgatcgagt cgacgtgcgg tggccgaggc acgtgcggca
     6901 agtgcggggt gaaggtgctc tcgggtcacg tggagcgctc gctcgccgac taccgccacc
     6961 tgaacgatca gctcgacgag ggctggcgcc tctcgtgcca ggcggagatc accgaggaca
     7021 ccgagtgcga tgtgccccgc ctgatgaaga tgcccaaggc ggccacgatg ggcgtcggac
     7081 gcttcgtgct gctcgagccc aacgtggtca agctcttcct cgagctgccg ccgccgtccc
     7141 tcgaggacca tcgctcccac ctcgcccggg tcctcgacgc cgtcgatgag gccgggtaca
     7201 accgcaacta cgactgggag gtcatgccga ggatcgcaca ggcgttccgc gcgacctcga
     7261 atgtgacggc gacgctggtg ggggagtacc tcgtcgatgt ggaacccggc gacaccaccg
     7321 atcgcatgtt cggcgcgtcg ttcgacatcg gcacgacgac gtgcgtctgc acgctcgtcg
     7381 acctgcgcaa cggggcgacg gtcggcgtgg cgagcacgat caaccaccaa gcgccgttcg
     7441 gggccgacgt gatcgcgcgc atggccaagg cgatgcacgg cgaggagaac gtcgtgcagc
     7501 tgcgcgaggc cgcgatctcc accgtgaacg agctgctcgg tcgcgcccga gaggaagccg
     7561 gcgtggccca gcacgaggtc tacgaggcgg tcgtcgccgg caacgccacg atgatgcacc
     7621 tgctctgcgg cgtgaacccg gagtcgctgg cgctcgcgcc gtacgtggcc acattcaccg
     7681 agccccagga cctgcgcggg gaccaggcgc ggttcgacat gcacccgctc ggatgggtgg
     7741 cgctcttccc gtcgatcggc gcctacgtgg gcgccgacat cgtggccgac atcgtggcga
     7801 ccggcctcgt gcgcgactcg gagacccggc tgctcgtcga cgtcggcacg aacggcgaga
     7861 tcgcgtgcgg gaacgaggag cgcagcgtgg ccaccgcggc gcccgccggc ccggcgttcg
     7921 agggcggcga gatcgtgcac ggcatgcggg cgaccgacgg cgcgatcgaa ggggtcaccc
     7981 tcacgaacgg cgaggtcgag ctgcaggtga tcggcggcga ggacgccgaa ccccgcggca
     8041 tctgtggctc ggggctgatc gacatcgtgg cccagctgcg gctggcgggt ctgctcgacg
     8101 acggcggaaa gatgatgtcg cgcgaggagg ccgaggacgc cggccactcc ctcgccgatc
     8161 gcctggtgat gcgcgaggac gtgaaggcgt tccacctgca cggcgacgtc gtgctgaccc
     8221 agcaggacgt gcgcgcgctg cagttcgcca agggcgcgat ctctacgggc atcgagaccg
     8281 cgatgcgcgc cctcggcctc gagccgtccg acctcgacgc ggtgatgctc gcggggtcgt
     8341 tcgggtccta catcaacccg cagagcgcgc gcgtgatcgg cctcgtgccc cccgtgcccg
     8401 tcgagcgcat caaggccgtc ggcaacacgg cgagcgaggg cgcgaagatg gcgctgatgt
     8461 ccttccgcga gcgcgaggtc gcgtgggaga tccccggcat cgtggagtac atcgagctct
     8521 ccggcgagga agacttcaac gaccgcttca tcgggaacct cgggtttccc ccgatcgagg
     8581 acctgcacgc cgcccaggcc gcgaacgagg ccgcgatcgt ggcgcgggcc gaggcgaggg
     8641 cgtcggcgag cgtcggctcg gaggacacga gcccgtgacc gcccccggtg ctcgcgtggc
     8701 tctgctcgcg tgcggcgccg tcgcgatcga cacgaagacg ctcgtcgagc gccacggctg
     8761 gaacgccgat gtgcacggca tctcgagcga cctgcacatg accccgctcg agatcgcgcc
     8821 ggcggtcgag gagaagctca tcgcgctgct gccccggtac gagcgcgtga tcgtcgtcta
     8881 cggcgactgc ggcaccggcg gtcggctcga cgaggtgcta tcgcgatacc cggcggtccg
     8941 gccagcgggg gtgcattgct tccagtggta ctccggcgag ctgtaccggc ggttcgagac
     9001 cgacatcggc atctacttcc tgaccgactg gctcgtgacg aactgggacc gggcggtgat
     9061 caaggggctc ggcctcgacc gcttcccctg gctgcgcgag acgtacttcg gcaacctgag
     9121 ccgggtgctg ttcgtgcgcc agcaccccga ccccgaacgc gaggcgaagg cgcgggagat
     9181 cgccgagtac atgcagctgc ccctcgagat ccacgacctc ggcatcgagc ccctcga
//
LOCUS       DATDBZ010000487         2154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_144073, whole genome shotgun sequence.
ACCESSION   DATDBZ010000487 DATDBZ010000000
VERSION     DATDBZ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144073"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VIX62_11435"
     CDS             complement(<1..654)
                     /locus_tag="VIX62_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5860890.1"
                     /translation="MDETTFSFDAADGVSVSAFRWTNVAPPKAIVQIAHGMGEHAARY
                     RRLAEALTAAGYVVYANDHRGHGTTAGTPEGFGDLGAKGWAGLVSDLGELGVIARREH
                     PGLPLVVLGHSMGSFALQGYLLDHSADVDAAVLSGTTAIDVIAPGIDPTKEVDLSAFN
                     APFEPARTEYDWLSRDPDEVDKYLADPACGFGLNPAATASMLDALADTGDAARLAGIR
                     "
     gene            complement(721..>2154)
                     /locus_tag="VIX62_11440"
     CDS             complement(721..>2154)
                     /locus_tag="VIX62_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY5860891.1"
                     /translation="VAVFGEGAMLTGEGRSRTAVVVRDSLLARIPPAVFEDVMIASPE
                     VAVAMARRVARRTVFPRVDDRARRRIDATVSFVSASVPTARLEPFREVSAAAVGGAGQ
                     VLAFGRDRSAEVFDAVRHSDRVVVVADAMDRVDVRPMMDMVLTGIDPLAAPALELLVI
                     DRPRTAGGGRGAVWRHPDGFSHHLRVREGDVADLRRIGRHVSGRSVGIVFGGGGARGL
                     AHIGVLRALAELDIPVDSVGGSSMGAIIGGQHAMGWSWERIYEHNRKAWGEHRVRFEI
                     SLPTVSLFSGRRSKRMYDAAFGDLDIEDFRLPFFCTSVDMSAFRLAIHRTGSAAQWIC
                     ASASVPGLWPPVVDAVGHLHVDGGQLNNVPTDVMRDAHDGPIIAVDVHAQQALMTVRP
                     DSRPPIGLRHILSRRAGRYPSIAETFNRCALLGSLQHQETARNYADIYLTPDLSDIGF
                     RAFHRIEHAAEIGYRTALQGLTGWTPP"
BASE COUNT          365 a          803 c          681 g          305 t
ORIGIN      
        1 gcggatcccg gcgaggcggg cggcgtcacc cgtatcggcc aatgcgtcga gcatgctggc
       61 cgtcgctgcc gggttcaggc cgaatccgca tgccggatca gcgaggtact tgtcgacctc
      121 gtcggggtcc cggctcagcc agtcgtactc ggtacgagcg ggctcgaacg gagcgttgaa
      181 ggcgctgagg tcaacctcct tcgtggggtc gatccccggc gcgattacgt cgatcgccgt
      241 cgtaccggag agcacggctg cgtccacgtc ggcgctgtga tcgagcaggt agccctgcag
      301 ggcgaacgaa cccatgctgt gacccagcac cacgagcgga aggccagggt gctctcgccg
      361 agcgataacc ccgagctcgc cgagatcgct caccagtccg gcccaaccct tcgcaccgag
      421 atcgccgaag ccctccggtg ttcccgcagt cgtgccgtgg ccgcgatggt cattggcgta
      481 cacgacgtaa ccggcggcgg tgagcgcttc ggccaatcgc cggtaccgcg cggcgtgctc
      541 acccatgccg tgcgcgatct gcacgatcgc cttcggtggc gcgacgttgg tccaccggaa
      601 ggccgagacg ctcacgccgt cggctgcatc gaatgaaaag gtcgtttcgt ccatggggac
      661 gatgatccca gatcgcggcc cacaggcgcg ccgtcgctcc ccctcaccaa cggccgagca
      721 tcacggcggc gtccagccgg tgagcccctg aagcgcggtc cggtagccga tctcggctgc
      781 gtgctcgatc cggtggaacg ctcggaagcc gatgtcgctg aggtcgggcg tgaggtagat
      841 gtcggcgtag ttgcgggcgg tctcctggtg ctgcaggctg ccgagcaggg cgcagcggtt
      901 gaacgtctcg gcgatgctcg ggtatcgacc ggccctgcgg ctgaggatgt gacgcagacc
      961 gatcggcggt cgcgaatcgg ggcggaccgt catcaacgcc tgctgggcat gcacatcgac
     1021 ggcgatgatc ggaccgtcgt gggcatcccg catgacgtcg gtgggcacgt tgttcaactg
     1081 gccaccatcg acatgcaggt gcccgacggc gtccaccacc gggggccaca acccgggcac
     1141 gctggcgctg gcgcagatcc actgcgctgc cgatcccgtg cgatggatgg cgaggcggaa
     1201 cgcgctcatg tcgacgctgg tgcagaagaa cggcagccgg aagtcctcga tgtcgaggtc
     1261 tccgaacgca gcgtcgtaca tccgcttcga gcgtcgcccc gagaacaacg agaccgtcgg
     1321 caacgagatc tcgaaccgga cccgatgctc cccccacgcc ttgcggttgt gctcgtagat
     1381 ccgctcccac gaccaaccca tggcgtgctg gccgccgatg atcgccccca tgctcgatcc
     1441 accgaccgaa tccaccggga tgtcgagctc ggccagcgcc cgcagcacac cgatgtgggc
     1501 cagcccccgg gcgccgcccc cgccgaacac gatcccgacc gagcgcccgc tcacatgccg
     1561 cccgatcctc cgcagatcgg cgacgtcgcc ctcgcgcacc cgcaagtgat ggctgaaccc
     1621 atcggggtgg cgccacaccg cgccccgacc accacccgca gtcctcggcc gatcgatcac
     1681 gagcagctcg agcgcaggcg ccgcgagcgg gtcgatgccg gtcaacacca tgtccatcat
     1741 cgggcgcaca tcgacgcgat ccatggcgtc ggccaccacg acgacccgat cactgtgccg
     1801 cacggcgtcg aagacctccg ccgatcgatc gcggccgaac gccaacacct gccccgcccc
     1861 gcccaccgcc gctgccgaca cttcccggaa cggctccaac ctcgccgtcg gcaccgacgc
     1921 cgacacgaac gacaccgtgg catcgatccg tcgacgcgca cggtcatcga cgcgcggaaa
     1981 caccgttcgc cgcgccacac ggcgcgccat cgccaccgca acctccggcg atgcgatcat
     2041 cacgtcctcg aacaccgccg gcggaatccg cgcgagcaga ctgtccctca ccaccaccgc
     2101 cgtgcgcgac cgaccttcac ccgtgagcat cgccccctcc ccgaacaccg cgac
//
LOCUS       DATDBZ010000488         4113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_568777, whole genome shotgun sequence.
ACCESSION   DATDBZ010000488 DATDBZ010000000
VERSION     DATDBZ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568777"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VIX62_11445"
     CDS             <1..777
                     /locus_tag="VIX62_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5860892.1"
                     /translation="ARFTIAADGAASRFAKPAGVRRDDSRPLGIAARRYYRTPYHPGP
                     WFESWLDLWEGDLLLPGYGWLFPVAGGQINLGAGLLNTFKDFKQISAQRLFNAFSTML
                     PPEWEIGEDTAEGRVLSGPLPMSLNRVPQVVPGMLLIGDAAGAVNPFNGEGIAYAMET
                     GEVAADLVHEALVKDRPGIAMMYPTVLRERYGDYFTIGRGFAKIIGRPAIMSRATRYL
                     LPNRRVMGFAMRVMANLTDGPEGDRQDRLFHLLQRIARAA"
     gene            794..1147
                     /gene="ndhC"
                     /locus_tag="VIX62_11450"
                     /pseudo
     CDS             794..1147
                     /gene="ndhC"
                     /locus_tag="VIX62_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
     gene            1308..1751
                     /locus_tag="VIX62_11455"
                     /pseudo
     CDS             1308..1751
                     /locus_tag="VIX62_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            1772..2272
                     /locus_tag="VIX62_11460"
     CDS             1772..2272
                     /locus_tag="VIX62_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY5860893.1"
                     /translation="MTPAELAAHVRERCPDAAVARGEVTAIVDRDDLLDVLVWLRDDP
                     GLSLGFCSSLTATDWPAADPRFWVAYELLSVEHHHRLRVKVGLAAADAHVPSVTPLFP
                     TANWHERETHDFYGIVFDGHPDPRPLLLPEGWSGHPLLKTEALGGVNTRFHGAFIPPV
                     DTRTTT"
     gene            2279..3565
                     /gene="nuoD"
                     /locus_tag="VIX62_11465"
     CDS             2279..3565
                     /gene="nuoD"
                     /locus_tag="VIX62_11465"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEY5860894.1"
                     /translation="MTDAPTTPITPDAPAVDARTGEIRQETMIINMGPQHPSTHGVLR
                     LLLELDGETVIKCTPIIGYLHTGIEKNTEYRTWMQGVTFVTRADYLSPFFNELGYCLA
                     VERLLGVEAPPRAQVIRVLMNELNRISSHLVWLATGGMELGAVSVMLYGFREREVLLD
                     IFEEVTGLRMNHAYIRIGGVIMDLPDEGVRKMRRFLEDMPARIDDYERLLDENPIWLE
                     RNVGVGVLSADDALALGVTGPVLRAAGVPLDVRKDAPYCGYETYDFDVPVRTEADSYA
                     RYRLRIEEMRESMKIVRQCLERLDEPGPVMIEDPKVAWPAKLSVGPDGIGNDPEYLRH
                     IMEESMEALIHHFKMVTEGVEVPPGEVYQAVESPRGELGFYVVSDGGHRPYRVKIRDP
                     SFVNLQAVPTMVEGSLVADAIAAIASLDPVMGGVDR"
     gene            3565..>4113
                     /locus_tag="VIX62_11470"
     CDS             3565..>4113
                     /locus_tag="VIX62_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY5860895.1"
                     /translation="MAIFEGVRLDEARSIVARYPEGRERSAVMPLLYLAQSIEGYVTR
                     DALREVAELIGVRTAEVEAVATFYTMLRLRPTGTHLIAVCTNLSCALRGANDVYAAAH
                     RATGIPDGGEVSEDGAFSVHEEECLGVCELAPVIQVNVANHDRVTPDGVADIVEALRA
                     GAVPPPARGPAIESFKAASRILA"
BASE COUNT          613 a         1393 c         1450 g          657 t
ORIGIN      
        1 gcccgcttca cgatcgcggc cgacggcgcg gcgagccgct tcgcgaagcc cgccggggtg
       61 cgccgcgacg actcccgtcc gctcggcatc gccgcccgtc gctactaccg cacgccctac
      121 caccccgggc cctggttcga atcgtggctc gacctgtggg agggcgacct gctgctgccg
      181 ggctacgggt ggctgttccc ggtcgccggc gggcagatca acctgggcgc cgggctgctc
      241 aacacgttca aagacttcaa gcagatctcc gcccagcggc tgttcaacgc gttctcgacg
      301 atgctgccgc cggagtggga gatcggcgag gacaccgccg aggggcgggt gctctctggc
      361 ccgctgccga tgagcctgaa ccgcgtgccc caggtcgtgc cgggcatgct gctgatcggc
      421 gacgccgccg gtgcggtgaa cccgttcaac ggcgagggca tcgcgtatgc gatggagacc
      481 ggcgaggtcg ccgccgacct cgtgcacgag gcgctcgtca aggaccggcc gggaatcgcg
      541 atgatgtacc cgaccgtgct gcgcgagcgc tacggcgact acttcacgat cggccggggc
      601 ttcgcgaaga tcatcggacg tccggcgatc atgagccggg ccacccgcta cctgctgccg
      661 aaccgccggg tcatggggtt cgcgatgcgc gtgatggcca acctcaccga cgggcccgag
      721 ggcgaccgcc aggaccggct cttccacctg ctgcaacgca tcgcgagggc agcgtgaccg
      781 ataggattcg cccgtgctga ccgagtacct gccgatcgtc gtcatggccg cgctggcgat
      841 cgtgttcggt gtggcctcgc tgctcgtgtc gagggcgttc cgcccgcatc gcccgaaccc
      901 cgtgaagctc tcggcgtacg agtgtggcaa cgatccggtt cgcctgccgc gcggcgagaa
      961 gttcagcgtg aagttctacg tcgtcgcgat gctgttcatc atcttcgaca tcgagatcat
     1021 cttcctgttc ccgtgggcgg tgcgcttccg ggagctgggg ctgttcggcc tcatcgagat
     1081 ggcgttcttc atcggcctcg tgttcatcgc ctacatctac atctggcgca cgggcgggtt
     1141 cgactggggt gacagcgtgg cgccgcccat cgagggcggg ctgcctcggg aagctgacgt
     1201 gatggccgag gccgacgccg acctcgcggc cgcgatgcgc gacacggagg ccgccggtgc
     1261 ccgttgagcg catcgagggt gtgcacctcg gcaagacccc cgaggagatc gaggacgagg
     1321 tccgtcgcgg ggtgctgctg acctcgctcg accgtgccgt cgggtgggcg cgcaagcagt
     1381 cgatgtggcc ggcgacgttc ggcctcgcgt gctgcgcgat cgagatgatg tcgaccggcg
     1441 ccggcaagta cgacctctcg cgctggggca tggagctgtt ccgtgcctcg ccgcgtcagg
     1501 ccgacctgat gatcgtggcg gggcgggtct cgcagaagat ggcgccggtg ctgcggcgca
     1561 tctacgacca gatgcccgag ccgcggtggg tgatctcgat gggggtgtgc gcgagcgccg
     1621 gcggcatgtt cacgaactac gcgatcgtgc agggggtcga cacgatcgtg ccggtcgacg
     1681 tgtacgtgcc ggggtgtccg ccgaagcccg agatgctgat gcacggcatc ctgaagctgc
     1741 acgagaaggt gcgcaccacg gagccgttca agtgacgccc gcggagctgg ccgcgcacgt
     1801 gcgcgagcgc tgccccgacg ccgcggtggc ccgcggcgag gtgaccgcca tcgtcgatcg
     1861 tgacgacctg ctcgatgtgc tggtgtggct gcgcgacgac cccggtctct cactggggtt
     1921 ctgttcgagc ctcacggcga ccgactggcc cgccgccgat ccgaggttct gggtggcgta
     1981 cgagctgctc tcggtcgagc atcaccaccg gctacgggtg aaggtgggcc tcgcggcagc
     2041 cgacgcgcac gtgccgagcg tgaccccgct attcccgacg gcgaactggc acgagcgcga
     2101 gacgcacgac ttctacggca tcgtgttcga tggtcatccc gacccgcggc cgttgctgct
     2161 gcccgagggc tggagcggcc atccactgct gaagaccgag gcgctcggcg gcgtgaacac
     2221 ccgcttccac ggggcgttca tcccgcccgt cgacacccgc acgaccacct gagaaccgat
     2281 gaccgacgct cccaccacgc cgatcacgcc cgacgccccg gcggtcgatg ctcgcacggg
     2341 cgagatccgc caggagacga tgatcatcaa catggggccg cagcacccct cgacccacgg
     2401 ggtgttgcgt ctgctgctcg agctcgacgg cgaaacggtc atcaagtgca cgccgatcat
     2461 cggctacctg cacacgggca tcgagaagaa caccgagtac cgcacctgga tgcagggcgt
     2521 cacgttcgtg acccgagccg actacctgtc gccgttcttc aacgagctgg ggtactgcct
     2581 ggcggtcgag cggttgctcg gggtcgaggc gcccccgcgc gcccaggtga tcagggtgct
     2641 gatgaacgag ctgaaccgca tctcctccca cctcgtgtgg ctcgccaccg gtggcatgga
     2701 gctcggcgcg gtgagcgtga tgctctacgg cttccgcgaa cgcgaggtgt tgctcgacat
     2761 cttcgaagag gtcacgggtc tgcgtatgaa ccacgcctac atccgcatcg gcggcgtgat
     2821 catggacctg cccgacgagg gcgtgcgcaa gatgcgccgc ttcctcgagg acatgccggc
     2881 gcgcatcgac gactacgagc ggctgctcga cgagaacccg atctggctcg agcgcaacgt
     2941 gggggtcggt gtgctctcgg ccgacgacgc cctcgcgctc ggcgtgaccg gcccggtgct
     3001 gcgcgcggcg ggcgtgccgc tcgacgtgcg caaggacgcc ccctactgcg gctacgagac
     3061 ctacgacttc gacgtgccgg tgcgcaccga ggccgactcc tacgcgcggt accgcctgcg
     3121 catcgaggag atgcgcgagt cgatgaagat cgtccgccaa tgcctcgagc gactcgacga
     3181 gcccggcccg gtgatgatcg aggatccgaa ggtggcatgg cccgcgaagc tctctgtggg
     3241 gcccgacggc atcggcaacg atcccgagta cctgcggcac atcatggaag agtcgatgga
     3301 ggcgctgatc catcacttca agatggtgac cgagggcgtc gaggtgccgc cgggcgaggt
     3361 ctaccaggcg gtggagtcgc cccgggggga gctcggcttc tacgtcgtgt ccgacggcgg
     3421 gcaccggccc taccgcgtga agatccgcga tccctcgttc gtcaacctcc aggccgtgcc
     3481 gacgatggtc gaggggtccc tcgtcgccga cgcgatcgcc gcgatcgcgt cgctcgatcc
     3541 cgtgatgggc ggggtggacc gctgatggcg atcttcgagg gggtgcgcct cgacgaggcg
     3601 cggtcgatcg tcgcccgcta cccggagggc cgcgagcgat ccgccgtgat gcccctgctc
     3661 tacctcgcgc agtcgatcga gggctacgtg acgcgtgacg cgctccgcga ggtggccgag
     3721 ctgatcggtg tgcgcacggc cgaggtcgag gcggtggcca cgttctacac gatgctgcgg
     3781 ttgcgaccga ccggcaccca cctgatcgcg gtctgcacga acctgtcgtg cgccctgagg
     3841 ggggcgaacg acgtctacgc ggcggcgcat cgcgcgaccg gcatccccga cgggggcgag
     3901 gtcagcgagg acggcgcgtt cagcgtgcac gaagaggaat gcctcggcgt gtgcgagctc
     3961 gcgcctgtca tccaggtgaa cgtcgcgaac catgaccggg tcacccccga cggcgtcgcc
     4021 gacatcgtcg aggccctgcg ggccggtgcg gtgcccccac ccgctcgtgg cccggcgatc
     4081 gagagtttca aggccgcgtc gcgcatcctc gcg
//
LOCUS       DATDBZ010000489         8741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1384343, whole genome shotgun sequence.
ACCESSION   DATDBZ010000489 DATDBZ010000000
VERSION     DATDBZ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384343"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VIX62_11475"
     CDS             <1..782
                     /locus_tag="VIX62_11475"
                     /inference="COORDINATES: protein motif:HMM:NF039285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY5860896.1"
                     /translation="ALFVVYIRGGNAQAEGALLFLALGGVGVGMTVWATRLMPRFRDE
                     THPRRHDEPATQQEMDAAVDDMGSGVEEIRRRRFLSRLLAGAAGALGLAALIPIRSLG
                     SAPGSSLFTTQWTEGARLVLDDGSPVTRDTLEVGSFTTVFPEGFVGSADSQAVLIRVE
                     PGQLQLPPDRLAGAPEGLVVYSKICTHAGCPLGLYLAETHELRCPCHQSTFDVLDGAE
                     PVYGPAPRPLPQLLIEIDADGGLRATGDFTAPVGPSFWDGP"
     gene            779..2182
                     /locus_tag="VIX62_11480"
     CDS             779..2182
                     /locus_tag="VIX62_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEY5860897.1"
                     /translation="MIVRRLIDGVDDRLDSTSFLRRSLHKVFPDHWSFMLGELTLYSF
                     VLLLLTGTFLSMFYVASPETTTYTGPYAPMAGRTVSLAYDSVMRISLEVRAGLVMRQT
                     HHWSALMFMAAMVAHLVRVFFTGAFRKPREMSWLIGVTMLVSGIGAGFTGYSLPDDLL
                     SGTGLRIIYSAVLSIPLIGTWLAYLFFGGEFPAPELLPRLFVMHVLFIPALLVGLLSA
                     HLALVWRQTHTQFPGPGTTEETVTGTPVWPKFALKAIGLAFVTFGVLTMLGGLVQINP
                     IWLYGPFQPYNAPSPAQPDFYAGWLEGLLRLWPNWEFHLFGHTIGELFLPSVVVPGII
                     FTVLALWPFLEARAKRDTATHHYAQRPREAPVRSAFGAGGIAFFVLLMLAGSNDVLAK
                     FLQVEVDTLNTVLKVLLFVVPVIAGVLTWWICRDLRARDERPIKAPSRVVFRRNAAGG
                     FDEEPDPSTTDHERTPP"
     gene            2206..2925
                     /gene="coxB"
                     /locus_tag="VIX62_11485"
     CDS             2206..2925
                     /gene="coxB"
                     /locus_tag="VIX62_11485"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY5860898.1"
                     /translation="MATIAAATTACSSSFGMPRGSSSQGEEVFSLWQIFFWAAIGVAA
                     IVYGLLGWSLVRYRRRRGDDDTALGRQFHANIPLEIVYTAIPVVIVVVLFTLSVRGDD
                     RVSAVSADPDVTLHVEAYQWGWRFSFPDEGVVVLSHPSGEGIPGPELWLPIGETVRIE
                     LTSNDVIHAFWVPDFLFKRDAIPGHVNVFDVSPTTAGTYHGVCSEFCGLNHSFMTFTV
                     KVVSPEEFERWLGEQSSEAAA"
     gene            2922..4556
                     /gene="ctaD"
                     /locus_tag="VIX62_11490"
     CDS             2922..4556
                     /gene="ctaD"
                     /locus_tag="VIX62_11490"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY5860899.1"
                     /translation="MSRAPMHRDGPVAWLTTTDHKKIGILYCVTAFGFFLIGGTLADV
                     MRTELAETGRQLVTPQAYNGLFTIHGIVMIFLFVAPFGIGLANYLIPLQIGAPDMAFP
                     RLNALSYWFFVGGGLLVLLSGFASAGGGAQTGWYMYAPLSGLRYSPGVGPDLLILGLA
                     LVAISTLLSGVNVITTTFLMRAPGMTMWRLPIFTWNMVVTSILGLVAFPPALAAFVLL
                     LFDRRLGGFAFDPQGGGDPIIYQHLFWFLGHPEVYILILPFFGVITEIIPVFSRKPLF
                     GYTGFVLATLAIGALSTGVWAHHMFTTGEVDNPFFSAMTLLIAVPTGVKFFNWIGTMW
                     GGRIRFGVPMLFSIGFLLNFLIGGVTGVMLASAPIDYSVSDSYFLVSHFHYTMMGGSV
                     FGIFAAIYFWWPKMTGYLLSERMGRAVFGLLFVGFNLTFWPQFVLGLRGMPRRIVDYA
                     SDLGWDTPNLVSTMGAGVLTIGVLVFLTDVWRSRRRRTPAGDDPWGGYSLEWATTSPP
                     PEHNFSSMPRIRSERPAFDLHHPKEPSIEAAEAETR"
     gene            4553..4936
                     /locus_tag="VIX62_11495"
     CDS             4553..4936
                     /locus_tag="VIX62_11495"
                     /inference="COORDINATES: protein motif:HMM:NF023691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEY5860900.1"
                     /translation="MRFALRYFALLSAFGLGLGTIYWFLTYEVVGAVVLWCFGLMPLI
                     VATWWWRQGTDPATEASDDPEASPSDAAGHTVGSFPLASAWPIFLVLGVIVTGASLVY
                     GLILLPAGAALLLWAIVGLARESRL"
     gene            complement(4946..5665)
                     /locus_tag="VIX62_11500"
     CDS             complement(4946..5665)
                     /locus_tag="VIX62_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY5860901.1"
                     /translation="MAEAAIHQVEWKGRTGPFSLHVRPGVFSPTTTSRTLADALEINP
                     GDTVIDVGCGSGVLAFVAAKLGAGKVYGCDLSGPAVRAARENAAMLGLSDVCEFRQGD
                     LLEPVRDVHADVLIGDVSGIPDELAAVTGWFDRVPAGGPTGAELPRKLLGSIGETLAP
                     GGRLYLPTGTLQDEASILTLARELFGDNMEPVLEREFPLPDLVAKATATARMMKEGLL
                     SLHKRGSRLLWSLRIWRCVRT"
     gene            complement(5700..6191)
                     /locus_tag="VIX62_11505"
     CDS             complement(5700..6191)
                     /locus_tag="VIX62_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEY5860902.1"
                     /translation="MPEEATGKTGMPSEKRQKPERPRDALGRPLDWDATNELEMEDYD
                     SLSMERNHELGREAMRARRFFPAHEAWETAWKQARGTDEAEFFKGLSQMGAGYVHLLR
                     GNAHGAITLMRRASSRVVHYPDGHRGIHTQALHDRLEADAAAVERGDLVPGPDVIFEP
                     IDV"
     gene            6262..8382
                     /locus_tag="VIX62_11510"
     CDS             6262..8382
                     /locus_tag="VIX62_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860903.1"
                     /translation="MGNTTVTLVPHTHWDREWYEPFSVFSERLVEMMDGLLALGDDGF
                     PHFHLDGQTAMIDDYLDRRPERQADLRRLAASGQLSVGPWVTQMDEFLTSGESHIRNL
                     EMGLARARDLAGERALDIGYMPDQFGHIGQMPQILRQGGLDRAMVWRGVPASIDRTSF
                     WWEAPDGSRVLTEYMVFGYFFGGSFLRAAEPSELAETLEASAGKLGPYLVSDRMVVMV
                     GYDHAGPDASLPGKVADAATLTPHMDVTIAGIAEHVHGQPSTEIPTWRGELRSSARAH
                     LLPNVYSARVHQKRERGRLEALIERSAEPLAAQVPGFGWPGDELDRAWTLLLWNGAHD
                     SACGCSHDQVAIDVDARFAEVREIGTDIVQRALESLGSRVAEAGVIRWNPSPFEREGV
                     PGNGWSVSPAGREPALLPVAIEILPDSDGVVADGIALRLFDEPDVGDLYTFCYAEEGQ
                     TPSPPASIELEGHEVRARWDGVLVVLRIVHRADEPFLRLEGTIHNERPDHRLRLHVGI
                     EGPVTGSVAGSPFELVERPLVGEGSGVESASTTWPARGVVMAGGVAVLHEGVFEYEVT
                     DRALAISLLRCVGTISRDVLATRPFQAGPPTPTPDAQMLGVTTFALGIRARATPETLL
                     TDHERFALTLAEAIAVGGGDLASTGTFRSIEGDAALSSIRRVGGSLQVRVWNPRSDAD
                     ITVTIDGEVHRLGPARIETLEVDG"
     gene            8379..>8741
                     /locus_tag="VIX62_11515"
     CDS             8379..>8741
                     /locus_tag="VIX62_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5860904.1"
                     /translation="MSERYDVIVVGGGAMGTAAARNLAMRGRRTLLLERFTFGHDKGS
                     SGGPTRIFRYAYHATSYVRMAMQARPAWDELQEAAGEELLRITGGLDVGPAALPRAEL
                     LEAAGAAVERWSAGQVRER"
BASE COUNT         1195 a         3015 c         3014 g         1517 t
ORIGIN      
        1 tcgccctgtt cgtcgtctac atccgcggcg ggaacgccca ggccgagggt gcgctgctgt
       61 tcctggcgct gggcggcgtg ggcgtcggca tgacggtgtg ggcgacgcgg ctgatgccgc
      121 ggttccgcga cgagacccac cctcgtcgcc acgacgaacc cgcgacccag caggagatgg
      181 atgccgccgt cgatgacatg ggatcgggcg tcgaagagat ccggcgtcgg cgcttcctct
      241 ccaggctgct cgccggagca gcgggggcac tcggtctcgc ggcgctgatc ccgatccggt
      301 cgctgggcag cgcgcctggc tcgtccctgt tcacgaccca gtggacggag ggcgcccgcc
      361 tggtcttgga cgacggctcg cccgtgacgc gtgacacgct cgaggtcggc tcgttcacga
      421 ccgtcttccc cgaagggttc gtgggctcgg cggactccca ggcggtgctg atccgcgtcg
      481 aacccggcca gctgcagctg ccgcccgacc ggctcgccgg tgcacccgag ggcctcgtcg
      541 tgtactcgaa gatctgcacg cacgccgggt gcccgctggg cctctacctg gccgagaccc
      601 acgagcttcg gtgcccgtgt catcaatcca cgttcgacgt gctcgacggc gccgagccgg
      661 tctacggccc ggcaccgcgg ccgctgcccc agctgctgat cgagatcgac gccgacggcg
      721 gcctgcgtgc caccggcgac ttcaccgccc ccgtgggccc gagcttctgg gacggaccgt
      781 gatcgtccgg cgactgatcg acggggtgga cgaccggctc gactcgacgt cgttcctgcg
      841 caggagcctg cacaaggtct tccccgacca ctggtcgttc atgctgggcg agctgacgct
      901 gtacagcttc gtgctgctgc tcctgaccgg cacgttcctc agcatgttct acgtggcctc
      961 gcccgagacc accacctaca ccgggcccta cgccccgatg gcgggacgca cggtgtccct
     1021 ggcctacgac tcggtgatgc gcatctcctt ggaggtccgg gccgggttgg tgatgcggca
     1081 gacccaccac tggtccgccc tgatgttcat ggccgcgatg gtggcgcacc tggtgcgcgt
     1141 gttcttcacg ggcgcgttcc gcaagccgcg cgagatgagc tggctgatcg gcgtcacgat
     1201 gctcgtgagc gggatcggcg ccgggttcac cggctattcg ctgcccgatg acctgctgtc
     1261 gggaaccggg ctgcgcatca tctactcggc ggtcctttcg atcccactga tcggcacgtg
     1321 gctcgcctac ctcttcttcg gtggtgagtt ccccgccccc gagttgttgc ccaggttgtt
     1381 cgtgatgcac gtgctgttca tccccgcgct gctcgtgggt ctgctgtcgg cccacctggc
     1441 cctggtgtgg cgccagacgc acacgcagtt ccccgggccg gggacgaccg aggagaccgt
     1501 caccggcacg ccggtgtggc ccaagttcgc gctgaaggcg atcggcctgg cgttcgtgac
     1561 gttcggcgtg ctgacgatgc tgggcggcct ggtgcagatc aacccgatct ggctctacgg
     1621 gccgttccag ccctacaacg cgccgtcgcc ggcccaaccc gacttctatg ccggctggct
     1681 ggaaggactg ctgcggctgt ggccaaactg ggagttccac ctgttcggtc acacgatcgg
     1741 ggagctgttc ctgccatcgg tcgtggtgcc gggcatcatc ttcaccgtgc tcgcgctctg
     1801 gccgttcctg gaggctcgag ccaaacgcga caccgccacg caccactacg cgcagcgacc
     1861 ccgggaggct ccggtgcgga gcgcgttcgg cgccggcggg atcgccttct tcgtgttgct
     1921 gatgctcgcc ggctcgaacg acgtcctggc caagttcctc caggtcgagg tggacacgct
     1981 caacaccgtg ctgaaggtcc tgctgttcgt cgtgcccgtg atcgcgggcg tcctcacgtg
     2041 gtggatctgc cgtgaccttc gcgcccgcga cgaacgaccg atcaaggcgc cgtcccgggt
     2101 ggtcttccgc cgcaacgccg ccggtgggtt cgatgaggaa cccgacccgt ccaccaccga
     2161 tcacgagcgg acccctccgt gacgcgtcgc cggtggatgg tcctcgtggc gacgatcgcg
     2221 gccgcgacca cggcgtgctc gtcctcgttc ggcatgccgc ggggcagttc gagccagggc
     2281 gaggaggtct tcagcctgtg gcagatcttc ttctgggccg cgatcggggt cgcggcgatc
     2341 gtctacggcc tgctggggtg gtcgctcgtc cgctatcggc ggcgccgtgg cgacgacgac
     2401 accgcgctcg gtcgccagtt ccacgcgaac atcccgctgg agatcgtcta cacggccatc
     2461 cccgtggtga tcgtggtggt gctgttcacc ctctcggtgc gtggcgacga ccgcgtctcg
     2521 gccgtctcgg ccgacccgga cgtcacgttg cacgtcgagg cctaccagtg gggatggcgg
     2581 ttctccttcc ctgacgaggg ggtcgtggtc ctgagccatc cgagcgggga gggcatcccc
     2641 ggacccgagc tgtggctgcc gatcggagag accgtccgca tcgagctcac ctccaacgat
     2701 gtgatccacg cgttctgggt gccggacttc ctgttcaagc gcgatgcgat ccccggtcac
     2761 gtgaatgtgt tcgacgtgag ccccacgacc gccggcacct accacggcgt gtgctcggag
     2821 ttctgtggac tgaaccactc cttcatgacg ttcaccgtca aggtcgtgtc ccccgaggag
     2881 ttcgagcgct ggctgggcga gcagagctcc gaggctgcgg catgagtcgc gcgccgatgc
     2941 atcgcgacgg cccggtggcc tggctcacca ccaccgacca caagaagatc ggcatcctct
     3001 actgcgtcac ggcgttcggc ttcttcctga tcggcggcac gctcgccgac gtgatgcgca
     3061 ccgagctcgc cgagaccggc cgtcagctcg taacgccgca ggcctacaac gggctgttca
     3121 cgatccacgg gatcgtgatg atcttcctgt tcgtcgcgcc gttcgggatc gggctggcga
     3181 actacttgat cccgctgcag atcggcgcgc ccgacatggc gttcccgcgg ctcaacgcgc
     3241 tgtcgtactg gttcttcgtg ggcggcgggt tgctggtgct gctgtcgggg ttcgcctcgg
     3301 ccgggggcgg cgcgcagacc ggttggtaca tgtacgcgcc gttgtcgggg ctgcgctact
     3361 cccccggcgt gggaccggat ctgttgatcc tgggcctcgc cctggtcgcg atctcgacgc
     3421 tgctgtcggg cgtcaacgtc atcaccacga cgttcctgat gcgcgcgccc ggcatgacga
     3481 tgtggcgcct gccgatcttc acctggaaca tggtggtcac ctcgatcctg gggctggtgg
     3541 cgttcccgcc ggcgctcgcg gccttcgtgc tgctgctgtt cgaccggcgt ctcggcgggt
     3601 tcgcgttcga tccacagggc ggcggcgacc cgatcatcta ccagcacctg ttctggttcc
     3661 tcggccaccc ggaggtctac atcctgatcc tgccgttctt cggggtgatc accgagatca
     3721 tcccggtctt cagccgcaag ccgctgttcg gctacaccgg gttcgtgctg gcgacgctcg
     3781 cgatcggcgc gttgtcgacc ggggtgtggg cccaccacat gttcaccacg ggtgaggtcg
     3841 acaacccgtt cttcagcgcg atgaccctgc tgatcgccgt gccgaccggc gtgaagttct
     3901 tcaactggat cggcacgatg tggggcgggc ggatccggtt cggtgtgccg atgctgttct
     3961 cgatcgggtt cctgctgaac ttcctgatcg gcggcgtgac cggcgtgatg ctggcgagcg
     4021 cgccgatcga ctactcggtg agcgactcct acttcctggt ctcccacttc cactacacga
     4081 tgatgggcgg ctcggtcttc gggatcttcg ccgcgatcta cttctggtgg ccgaagatga
     4141 ccggctacct gctgagcgaa cgcatgggcc gggcggtgtt cggactgctg ttcgtggggt
     4201 tcaacctcac gttctggccg cagttcgtgc tggggctccg cggcatgcca cgacggatcg
     4261 tggactacgc gagcgacctg gggtgggaca caccgaacct ggtgtccacg atgggcgccg
     4321 gagtgctcac gatcggggtg ttggtgttcc tcaccgatgt gtggcgatcg cgtcgtcgtc
     4381 ggacccccgc cggggacgac ccgtggggtg ggtactcgct ggagtgggcg actacgtccc
     4441 cgccgcccga acacaacttc tcgtcgatgc cgcggatccg ctccgaacgc cccgccttcg
     4501 acctgcacca cccgaaggaa ccgagtatcg aggcggcgga ggccgagaca cggtgaggtt
     4561 cgcactccga tacttcgcgt tgctgtcggc cttcgggctc ggactgggga cgatctactg
     4621 gttccttacc tacgaggtgg tcggcgcggt cgtgctgtgg tgtttcgggc tgatgccgtt
     4681 gatcgtggcg acatggtggt ggcgtcaggg caccgacccg gccaccgagg cctccgacga
     4741 tcccgaggcc tccccctcgg acgcggcggg ccacaccgtg gggtcgttcc cgctggcctc
     4801 ggcctggccg atcttcctgg tgctcggcgt gatcgtgacc ggggcgagcc tggtgtacgg
     4861 gttgatcctg ttgccggcgg gtgcggcgct gctgctgtgg gcgatcgtcg gcctggcgcg
     4921 cgagagccgc ctgtagcggc gcccgtcagg tgcgcacgca gcgccagatg cggaggctcc
     4981 acaggagccg gctgccgcgt ttgtggaggc tcaggaggcc ctccttcatc atgcgcgccg
     5041 tcgccgtggc cttggccacc aggtcgggca gcgggaactc ccgctcgagc accggttcca
     5101 tgttgtcccc gaacaactcg cgggcgagcg tgaggatcga ggcctcgtcc tgcagcgtgc
     5161 cggtcggcag gtagagccga ccgcctggag cgagcgtctc accgatcgag cccagcagct
     5221 tgcgcgggag ctcggcgccc gtgggaccgc cggccggaac ccggtcgaac cagccggtga
     5281 cggctgcgag ttcgtcgggg atgccggaca cgtcgccgat caacacgtcg gcgtgcacgt
     5341 cacggaccgg ttcgagcaga tcgccctggc ggaactcgca cacatcggag agcccgagca
     5401 tggccgcgtt ctcgcgcgcc gcgcggacgg ccggcccgga caggtcgcag ccgtacacct
     5461 tgccggcgcc gagcttggcg gcgacgaacg ccagcacccc gctgccgcac ccaacgtcga
     5521 tcaccgtgtc gcccggattg atctccaacg cgtcggccag cgtgcggctc gtggtggtgg
     5581 ggctgaacac ccccggacgg acgtgcaggc tgaacggacc ggtgcggccc ttccattcca
     5641 cctggtggat cgcggcctct gccatggttt gaaggctaca cgtatgcgac gcaacgcggc
     5701 tagacgtcga tcggctcgaa gatcacgtcg ggcccgggca cgagatcgcc ccgttcgacg
     5761 gcggccgcat cggcttcgag ccgatcgtgc agcgcctgcg tgtggatgcc gcggtggcca
     5821 tcggggtagt gcaccacccg gctcgatgcc cggcgcatca gcgtgatggc cccgtgggcg
     5881 ttcccgcgga gcaggtgcac gtagccggca cccatctgcg agagcccctt gaagaactcg
     5941 gcctcgtcgg tgccgcgggc ctgcttccag gccgtctccc atgcctcgtg ggccgggaag
     6001 aaccggcggg cccgcatcgc ctcccggccc agttcatggt tccgctccat ggacagcgag
     6061 tcgtagtcct ccatctcgag ctcgttcgtc gcatcccagt ccagggggcg accgagcgcg
     6121 tcacggggac gttcgggctt ctgacgcttc tcgctgggca tcccggtctt gccggtggct
     6181 tcctcgggca tggggccatg ctactcgggg gtctcgatcc ggacacgacg agcgctccac
     6241 cccgtggcac actctggctc catggggaac acgaccgtca ccctggtccc gcacacgcac
     6301 tgggatcgtg aatggtacga acccttcagc gtcttctccg agcggctcgt cgagatgatg
     6361 gacgggctcc tcgccctcgg cgacgacggg ttcccgcatt tccacctcga cggtcagacc
     6421 gcgatgatcg acgactacct cgatcgccgg cccgaacgcc aggcagacct gcgccggctc
     6481 gcagcgtcgg gccaactctc tgttggaccg tgggtcacgc agatggacga gttcctcacc
     6541 agcggggaat cgcacatccg caacctggag atgggcctgg cccgcgcgcg tgatctcgcg
     6601 ggcgaacgcg cgctcgacat cggctacatg cccgaccagt tcggccacat cggccagatg
     6661 ccacagatcc tgcgacaggg cggcctggac cgggcgatgg tgtggcgcgg cgtgcccgcc
     6721 tcgatcgatc gcacgtcgtt ctggtgggag gcccccgacg gcagccgggt cctcaccgag
     6781 tacatggtgt tcggctactt cttcggcggc agcttcctcc gcgcggccga gccctcggag
     6841 ctggccgaaa ccctcgaggc cagcgccggg aagctcggcc cgtacctggt cagcgatcgc
     6901 atggtggtga tggtgggcta cgaccacgcc ggtcccgacg cctcgctccc cggcaaggtc
     6961 gccgacgccg ccacgctgac ccctcacatg gatgtcacga tcgcggggat cgccgagcac
     7021 gtccacggcc aaccttcgac cgagatcccc acctggcgag gggagcttcg ttcttcggcg
     7081 agggcccacc tcctgcccaa cgtctactcg gcccgtgtcc accagaagcg cgaacgcgga
     7141 cgcctggagg cgctgatcga acgctccgcc gagccgctcg cggcacaggt gcccgggttc
     7201 ggatggcccg gagacgagct cgatcgcgca tggacgctcc tgctgtggaa cggcgcgcac
     7261 gactcggcgt gcgggtgctc ccatgaccag gtggcgatcg acgtcgacgc ccgcttcgcc
     7321 gaggtgcgcg agatcggcac cgacatcgtg cagcgcgcgt tggagtcgct cggctcccgc
     7381 gtcgccgaag ccggggtgat ccgctggaac ccgtcgccgt tcgagcgcga gggcgtgccg
     7441 gggaacggtt ggtcggtgtc gccggcgggg cgcgagccgg cgctgctccc ggtcgcgatc
     7501 gagatcctgc ccgacagcga tggcgtggtc gccgacggca tcgcgctccg cctgttcgac
     7561 gaacccgacg tcggcgacct ctacacgttc tgttacgcgg aggaagggca aacaccgtcg
     7621 cccccggcct cgatcgagct ggaggggcac gaggtccgag cgaggtggga cggcgtcctc
     7681 gtggtgctgc ggatcgtgca tcgagcagac gagccgttcc tgcgcctcga gggcacgatc
     7741 cacaacgaac ggcccgacca ccggctccgc ctccacgtgg ggatcgaggg accggtgacg
     7801 ggctcggtcg cgggctcacc gttcgaactg gtggagcgcc cactcgtggg cgaaggcagc
     7861 ggcgtggagt cggcctcgac cacctggccc gcgcgcgggg tcgtgatggc cggcggggtg
     7921 gcggtgctgc acgaaggggt cttcgagtat gaggtgactg accgggcgct ggcgatcagc
     7981 ctgctgcgct gcgtcggcac gatcagccgc gacgtcttgg ccacgcggcc gtttcaggcc
     8041 ggcccgccca cgcccacgcc ggatgcccag atgctcgggg tgaccacgtt cgcgctcggc
     8101 atccgagccc gggccacgcc cgagaccctg ttgaccgacc acgaacggtt cgccttgacg
     8161 ctcgcggaag ccatcgcggt gggtggcggc gacctcgcat ccacgggaac gttccgatcg
     8221 atcgagggcg atgcggcgct gtcatcgatc cgacgcgtgg gaggcagcct gcaggtgcgc
     8281 gtgtggaacc ctcgcagcga cgccgacatc accgtcacga tcgacggtga ggtccaccgc
     8341 ctcggccccg cccgcatcga gacgctggag gtggacggat gagcgagcgt tacgacgtca
     8401 tcgtggtggg cgggggcgcc atgggcaccg ccgccgcgcg caacctcgcg atgcgcggac
     8461 gccgcaccct gctgctcgag cggttcacct tcggccacga caagggcagt tcagggggac
     8521 ccacaaggat cttccgttac gcctatcacg caacgagcta cgtgcgcatg gcgatgcagg
     8581 cccggcccgc gtgggacgaa ctccaggagg cggccggcga ggagctcctg cgcatcaccg
     8641 gcggcctcga cgtggggccg gcggccctgc cccgagccga actgttggaa gcagccggcg
     8701 ccgcggtcga acggtggtcg gcgggccagg tgcgggaacg c
//
LOCUS       DATDBZ010000490         2398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_789276, whole genome shotgun sequence.
ACCESSION   DATDBZ010000490 DATDBZ010000000
VERSION     DATDBZ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789276"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VIX62_11520"
     CDS             <1..447
                     /locus_tag="VIX62_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005258616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEY5860905.1"
                     /translation="QAYAAGAALHFIEQALSEVRAGHTKTWEPFEVPDEGIGCGFTEA
                     VRGVLSHHMVIRGGKIANYHPYPPTPWNANPRDIYGTPGPYEDAVQDCPIFEENTRAN
                     FKGIDIMRTVRSFDPCLPCGVHMYLGGGKVLKKVHSPAMLNHPEFG"
     gene            478..1062
                     /locus_tag="VIX62_11525"
     CDS             478..1062
                     /locus_tag="VIX62_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY5860906.1"
                     /translation="MDGPINVRTIGDDVERLLDELQTRLEPRAYDQAVELVRLVTELY
                     GAGLGRVVELAGEALDEDAPAFVRRLAEDDLIGSLLLVHGLHPDSLGQRVAAALDGVR
                     PFLGGHGGDVELLEVDEDVGAVHLRLLGSCDGCPSSAMTLRLTVERAIMEAAPEISVI
                     DVDEPSADPVTSPVALTRKPAVAYDPATGCGAVR"
     gene            1068..1709
                     /locus_tag="VIX62_11530"
     CDS             1068..1709
                     /locus_tag="VIX62_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HEY5860907.1"
                     /translation="MSAVLKRIRATRPRELPRPGERCEVCAEMIPEDHDHVVDLDNRA
                     LLCACRPCWLVFSPQGSGGRRFRAVPDRYLAFPGTSLGGGWDQLDIPVGVAFFFLNST
                     TEQLAAFYPSPAGATESLLSLDAWNGIIEANPQLATLQPDVEAVLIRTEEAARADGSG
                     PECFIVPIDVCYELVGEMRRVWRGFDGGTEAHVVLRAFFDRIRGRSSEAAVAP"
     gene            1706..2344
                     /locus_tag="VIX62_11535"
     CDS             1706..2344
                     /locus_tag="VIX62_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004921518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HEY5860908.1"
                     /translation="MSPLSFSVVDARAEPHAAVPTLMLRLRIDAATPEPVHAVALRCQ
                     IMIEPQRRRYEPNEEERLTELFGETPRWGDTLRPFLWTNVGMTIPGFTGGTDVDLPIT
                     CTYDMDVAAVKYFHALRHGEIPLVLLFSGTAFSRGAAGLSAQPIAWHEEATFRLPVAT
                     WRAVIDMYFPNSGWLRLHEETLDALRRFRADRGLLTWDETLAVLMKEAGETP"
BASE COUNT          373 a          830 c          828 g          367 t
ORIGIN      
        1 caggcctatg ccgctggtgc cgccctccac ttcatcgagc aggcactgtc cgaggtgcgg
       61 gccggccaca ccaagacctg ggaaccgttc gaggtaccgg acgaggggat cggttgcgga
      121 ttcacggaag ccgtgcgggg cgtgctctcg caccacatgg tgatccgcgg cggcaagatc
      181 gcgaactacc acccctaccc cccgacaccg tggaacgcca acccgcgtga catctacggg
      241 acccccgggc cctacgagga cgccgtccag gactgcccga tcttcgagga gaacaccagg
      301 gcgaacttca agggcatcga catcatgcgg accgtccgca gcttcgaccc gtgcctgccg
      361 tgtggcgtgc acatgtacct cggtggcggc aaggtgttga agaaggtgca ctcacccgcg
      421 atgctcaacc accctgaatt cggctgagcc gcccgaaccc ggaagcgagg ccgccacgtg
      481 gacgggccga tcaacgtgcg caccatcggc gacgacgtcg agcgcctgct cgacgagctg
      541 cagacgaggc tcgaaccgcg cgcctacgac caggccgtcg agttggtgcg gctcgtcacg
      601 gagctctacg gcgccggcct cgggcgcgtc gtcgagctcg ccggggaggc gctcgacgag
      661 gacgctccgg cgttcgtgcg ccgcctcgcc gaagacgacc tcatcggcag cctgctcctg
      721 gtgcacggcc tgcatcccga cagcctcggc cagcgggtgg cggcggcgct cgacggggtg
      781 cggccgttcc tcgggggcca cggcggcgat gtcgagctgc tggaggtcga cgaggacgtg
      841 ggcgccgtcc acctgcggct gctcggcagt tgcgacggat gcccgtcgtc ggcgatgacg
      901 ctccggttga cggtcgagcg ggcgatcatg gaagcggcgc ccgagatcag cgtcatcgac
      961 gtcgacgagc cgtcagccga cccggtcacg tcgcccgtgg cgttgacccg caagccagcg
     1021 gtcgcttacg accccgccac gggctgtgga gcggtgcggt gacagaggtg agcgcggtcc
     1081 tgaaacggat ccgcgccacc cgcccgcgtg agctaccccg tccaggcgag cggtgcgaag
     1141 tgtgcgccga gatgatcccg gaggaccacg accacgtcgt cgatctggac aaccgcgcct
     1201 tgctctgcgc ctgccgtccc tgctggctcg tcttcagccc gcaggggtcg ggcggccgac
     1261 ggttccgcgc cgtacccgac cggtacctcg cgttccccgg aacgtcgctc ggaggtgggt
     1321 gggatcagct ggacatcccg gtcggggtgg cgttcttctt cctcaactcg acgaccgagc
     1381 agctcgccgc gttctacccg agccctgcgg gcgcgacgga atcgttgctg tcgctggacg
     1441 cgtggaacgg gatcatcgag gcgaacccac agctggccac gctgcagccc gatgtcgagg
     1501 cagtactcat ccggaccgaa gaagcagcgc gcgccgatgg ctctggaccg gagtgcttca
     1561 tcgtgcccat cgacgtctgc tacgagctcg tcggcgagat gcggcgcgtc tggcgcggct
     1621 tcgacggcgg cacggaggcg cacgtcgtac tgcgggcgtt cttcgaccgt atccgaggtc
     1681 gctcgagcga ggccgcggtg gcgccgtgag cccactgtcg ttctcggtcg tcgacgcccg
     1741 tgccgagccg cacgccgcgg tccccacgct catgctccga ctgcggatcg atgctgcgac
     1801 accggagccg gttcatgccg tcgcactcag gtgccagatc atgatcgagc cacaacgccg
     1861 gcgctatgag ccgaacgaag aggaacgact cacggagctg ttcggggaga cgcctcggtg
     1921 gggcgacacg ttgcggccgt tcctgtggac gaacgtcggc atgaccatcc cgggcttcac
     1981 cggtggcacg gacgtcgacc tcccgatcac gtgcacgtac gacatggacg tcgccgcggt
     2041 gaagtacttc cacgccctcc gccacggtga gataccgctc gtgctgctgt tctccggcac
     2101 cgcgttctcg cgcggcgccg cgggcctcag cgcacaaccg atcgcatggc acgaagaggc
     2161 gacgttccgc ctgcccgttg cgacgtggcg ggcggtgatc gacatgtact tcccgaacag
     2221 cggatggctg aggttgcacg aggagacgct cgatgccctc cggcgcttcc gtgccgatcg
     2281 aggcctgctg acgtgggacg agacgctcgc cgtgctgatg aaggaagcgg gggagacgcc
     2341 gtgaccgctg gtcccgtgac cggcgtcggt atcgaccgct tcgccgccgc ccgcgccg
//
LOCUS       DATDBZ010000491         2042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2210786, whole genome shotgun sequence.
ACCESSION   DATDBZ010000491 DATDBZ010000000
VERSION     DATDBZ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210786"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..490
                     /locus_tag="VIX62_11540"
     CDS             80..490
                     /locus_tag="VIX62_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860909.1"
                     /translation="MDERVSVQQGVVGAVSAGSVDIRNAGIGAVAGHDVSITNGGTGA
                     IAAAGDVNITNGGSGPIAALGSISITNGGAQTIATIGGATLHERSFVAFVLAPKVTIE
                     DGARVFMTTRQAMAAGAAFAVVLGVLTRRSRRRR"
     gene            complement(487..1512)
                     /locus_tag="VIX62_11545"
     CDS             complement(487..1512)
                     /locus_tag="VIX62_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY5860910.1"
                     /translation="MPHVDRYLSFALLARWSFVLAMVGSLLVGPTVIASAQSPSEATP
                     SAEPAPGASTADPEDIVVLSGTALVPRGQAAGEVGVFHGRAVVLGVVTGDVVVLDGSV
                     TVSGQVSGSVIAINGPIRVAATASIRGDVLGGQTVQVVEGATIEGDVRESVAFTPRGS
                     LSVLGSLIGPLAIACSVFLLGLALLAVLPRGADRVAAAARSAPLTSFAWGLVLAIALP
                     VLGAALSATVVALPVGLALLLGLALLFLVGATWAVWSVGRALVRDPRSRWLAYLAGWA
                     IAVAVSLVPYLNIAAWVVAAMFGLGAMMVATWRARSGAGRHRAGYVDPGAPGPPPTAA
                     AEPTTSA"
     gene            1581..>2042
                     /locus_tag="VIX62_11550"
     CDS             1581..>2042
                     /locus_tag="VIX62_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5860911.1"
                     /translation="MSAPPFSAAVRGWFESSFDEPTPAQSKGWKAIAGGDHTLILAPT
                     GSGKTLAAFLWAIDRLSTQPPPEPDRRTRVLYISPLRALAVDVEKNLRSPLAGIQHAA
                     AREGTTLPHDPQVGVRTGDTPTRDRRRLIAHPPDILITTPESLYLMLTSSAR"
BASE COUNT          351 a          766 c          676 g          249 t
ORIGIN      
        1 gggcagaccg gggctcggtc aacgtcgaac ctccagatcc gcgagaggat ggaagttcgc
       61 cgaacgaggg agcagcacga tggacgagcg agtctcggta cagcagggcg tcgtcggcgc
      121 ggtgtcggca gggtcggtcg atatccggaa cgccgggata ggcgcggtcg ccggacacga
      181 tgtatccatc acgaacggcg gcaccggggc gatcgcagcc gcgggcgacg tgaacatcac
      241 caacggtggt tctggaccca tcgcggcgct cggcagcatc tcgatcacga acggcggcgc
      301 gcagacgatc gcgaccatcg gcggcgccac tctgcacgag cgcagtttcg tcgccttcgt
      361 gctggcgccg aaggtcacga tcgaggacgg cgctcgggtg ttcatgacga cccggcaggc
      421 catggcggcc ggtgctgcct tcgcggtcgt gttgggcgtg ctgacgcggc gttcacgccg
      481 tcgtcgctag gcggacgtcg tcggttcggc cgccgctgtc ggaggcggcc cgggggcgcc
      541 cgggtcgacg tagcccgcgc ggtggcgtcc ggcgccgctg cgagcccgcc aggtcgccac
      601 catcatcgcg cccagcccga acatcgccgc cacgacccac gccgcgatgt tcaggtaggg
      661 aaccagactg accgcgacgg cgatcgccca ccccgccagg tacgcgagcc accgagatct
      721 cgggtcgcgg accagtgcac gccccacgga ccacaccgcc caggtcgcgc cgaccaggaa
      781 caacagtgcc agccccaaca agagcgccag tcccacgggg agcgccacca cggtcgccga
      841 cagcgcagcg cccagcaccg gcagggcgat cgcgagcacc agtccccatg cgaacgaggt
      901 gagcggcgcg gaacgggctg cggcggcgac acggtcggcg ccgcggggca ggaccgcgag
      961 cagcgccagc cccaggagga acaccgaaca cgcgatggcg agggggccga tgaggctacc
     1021 gagcacggag agggaaccgc gcggcgtgaa cgcgacggac tcccgcacgt cgccctcgat
     1081 cgtggcgccc tccacgacct gcaccgtctg gccgccgagc acgtcgccac ggatcgaggc
     1141 cgtcgccgcg acacggatcg gtccgttgat ggcgatcacc gagccgctca cctgacccga
     1201 caccgtcaca gagccgtcca gcaccaccac gtcgcccgtg accacaccca gcaccaccgc
     1261 ccgtccatgg aacaccccga cctcaccggc ggcttgtccc cggggcacca aggccgtccc
     1321 cgacagcacc acgatgtcct ccgggtcggc ggtcgaagcg cctggagctg gttccgcaga
     1381 gggtgtcgct tccgacggcg actgggcgct ggcgatgact gtaggcccaa ccaggagcga
     1441 tccgaccatc gcgagcacga acgaccagcg cgccagcaga gcgaaggaga ggtaccgatc
     1501 gacgtggggc atcgaggcga atcctaccct cgtccgcggt cgcctccgcg ttcgtcacag
     1561 agagccggta cggtgtgctg gtgagcgccc ctccgttctc cgccgcggtt cgcgggtggt
     1621 tcgaatcctc gttcgacgaa cccacgccgg cgcaatccaa ggggtggaaa gcgatcgccg
     1681 gcggcgatca caccctgata ctcgcgccca ccggttcggg caagaccctc gcggccttcc
     1741 tgtgggcgat cgaccggctc agcacccagc caccgcccga acccgatcgg cggacccgcg
     1801 tcctctacat ctcgccgttg cgtgcccttg cggtcgacgt cgagaagaac ctgcgctcgc
     1861 ccctcgccgg catccagcac gccgccgctc gtgaaggcac cacgctcccc cacgacccac
     1921 aggtgggcgt gcgcacgggc gacacgccca cccgcgatcg acgccggctc atcgcgcacc
     1981 cacctgacat cctcatcacc acgcccgaat cgctctacct catgctcacc tcctcggctc
     2041 gc
//
LOCUS       DATDBZ010000492         3169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1992758, whole genome shotgun sequence.
ACCESSION   DATDBZ010000492 DATDBZ010000000
VERSION     DATDBZ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992758"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VIX62_11555"
     CDS             complement(<1..226)
                     /locus_tag="VIX62_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY5860912.1"
                     /translation="MPIARTEFGSGLRVVTERMPSVRSVSLGFWVLAGSRDEAPKISG
                     SSHFLEHLLFKGTEHRTALDIAENFDAVGGD"
     gene            complement(239..2668)
                     /locus_tag="VIX62_11560"
     CDS             complement(239..2668)
                     /locus_tag="VIX62_11560"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY5860913.1"
                     /translation="MATTNLSRKIGNQTLTFETGKMARLADGAVMVTYGETQVLGTCV
                     SSNPREGIDFFPLTIDVEERMYAIGKIPGSFFRREGRPGETAILTARLIDRPLRPTFA
                     DGYRDEVQVVITVLGSDGDNPYDIPAMNAASVATCLAGLPFDGPVASVRVALIDGQWT
                     VNPTFAETDEATFDVVVAGRRSDSGEIDILMIEGEAPDKTWELLASGAQAPTEETVAE
                     GLEAAKRAIAEVLEFQQEFIDAAGVTPKEWTPRPLFGDDLYEALSSFAKSRLEESIVT
                     DKAEREAKLDTLKAEAKEHMAANFADFDTRSGEFGPAWKQLQKKVMRARVIAEGVRLD
                     GRGPKDIRPLSAEVGVLKRAHGSALFNRGDTQVLNITTLGMLRMTQMIDTLDLEDSKR
                     YMHHYNFPPYSTGETGRVGSPRRREIGHGALAERALIPVIPTEDEFPYALRLVSDVLS
                     SNGSTSMASVCGSTLSLMDAGVPIKAPVAGIAMGMIADDGKFVTLTDILGAEDALGDM
                     DFKVAGTREFVTAIQLDMKVTGLPGDVLAGALQQARDARFTILDVMEGAIAMPREEVN
                     AKAPRIISIQIPVDKIGEVIGPKGKRINEIIAATGADIDIQDDGTVFIGSELGGEGAD
                     EAAHMIDEIANPRPVLVGEQYDGVVVKTTTFGAFVNLVPGRDGLVHISKLGRGKRLSS
                     VEEAIKEGDKITVLVEDIDGQGKISLKPVGEEWAIPEGQADESGGGRPRGDRDRGGRG
                     GGGGGRDRGSSDRGASDRGSSDRSSGSGDRGERSRGRRFRDGEGASQAPGGESTPASA
                     DAPAGDAPSAE"
     gene            complement(2918..>3169)
                     /gene="rpsO"
                     /locus_tag="VIX62_11565"
     CDS             complement(2918..>3169)
                     /gene="rpsO"
                     /locus_tag="VIX62_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY5860914.1"
                     /translation="EKTQIITEYARADGDTGSADVQIAVLTKRIADLTEHLKTHKHDH
                     ASRRGLLKMVGRRRRLLDYLMHEDIERYRALIARLGLRR"
BASE COUNT          445 a         1093 c         1074 g          557 t
ORIGIN      
        1 tgtcaccgcc caccgcgtcg aagttctcag cgatgtcgag cgcggtccgg tgctcggtgc
       61 ccttgaagag caggtgttcc aagaaatggc tggacccgct gatcttcggg gcctcgtcgc
      121 gggaccccgc gagcacccag aagccgaggg acacggagcg gacgctgggc atccgctccg
      181 tgaccactcg caggcccgag ccgaactcgg tacgagcgat cggcatcgtt ggtgccggct
      241 actcggcgct gggcgcgtcg ccggcgggtg cgtcggccga agccggcgtg ctctcgccac
      301 cgggggcctg gctggcgccc tcaccgtcgc ggaagcgacg gcctcggctg cgctcaccac
      361 ggtcgccgga gccgctgctg cgatccgagg acccgcggtc ggaagcaccg cggtccgagg
      421 aaccacggtc gcggcctccg ccgccaccgc cgcgaccacc acggtcacgg tcgccgcgcg
      481 gacgtccgcc gccggattcg tcggcctgcc cctcggggat ggcccactcc tcgccgaccg
      541 gcttcaggct gatcttgccc tggccgtcga tgtcctcgac caggacggtg atcttgtcgc
      601 cttccttgat cgcctcctcg acgctgctca ggcgcttgcc gcgcccgagc ttggagatgt
      661 gcaccaggcc gtcccggccg ggcaccaggt tcacgaaggc gccgaacgtg gtggtcttga
      721 ccacgacccc gtcgtactgc tcgcccacca gcaccgggcg cgggttcgcg atctcatcga
      781 tcatgtgcgc cgcttcgtcg gcaccttcgc cgccgagctc ggagccgatg aacaccgtgc
      841 cgtcgtcctg gatgtcgatg tcggcgccgg tggccgcgat gatctcgttg atccgcttgc
      901 ccttgggtcc gatcacctca ccgatcttgt cgacggggat ctggatgctg atgatgcgcg
      961 gcgccttggc gttgacctct tcacgaggca tcgcgatcgc gccttccatg acgtccagga
     1021 tcgtgaagcg agcatcccgc gcctgctgga gcgcaccggc gagcacgtcg ccgggcagcc
     1081 ccgtcacctt catgtcgagc tggatcgcgg tgacgaactc ccgcgtgccg gccaccttga
     1141 agtccatgtc tcccaacgcg tcctcggcac cgaggatgtc ggtgagggtc acgaacttgc
     1201 cgtcgtcggc gatcatgccc atcgcgatgc cggcgaccgg cgccttgatc ggcacccccg
     1261 cgtccatcag cgacagcgtc gaaccgcaca cgctggccat cgaggtcgag ccgttcgagc
     1321 tcaacacgtc gctcaccagt cgcagcgcgt aggggaactc gtcctcggtc gggatcaccg
     1381 ggatgagggc ccgctcggcg agcgccccgt ggccgatctc gcgccggcgg ggcgaaccca
     1441 cgcggccggt ctcgcccgtg gagtacggcg ggaagttgta gtggtgcatg tagcgcttcg
     1501 agtcctcgag gtcgagggtg tcgatcatct gggtcatgcg gagcatgccc agcgtggtga
     1561 tgttcagcac ctgggtgtca ccacggttga acaacgccga accgtgggcc cgcttcagca
     1621 cgcccacctc ggccgacagc ggccggatgt ccttgggacc gcgaccgtcc aggcggacgc
     1681 cctcggcgat cacgcgagca cgcatgacct tcttctgcag ctgcttccag gcgggcccga
     1741 actcgccgct gcgcgtgtcg aagtcggcga agttggcggc catgtgttcc ttggcttcgg
     1801 ccttgagcgt gtcgagcttg gcttcacgct cggccttgtc ggtgacgatc gattcctcca
     1861 gccgcgactt cgcgaacgag gacagcgcct cgtacaggtc gtctccgaag aggggacgcg
     1921 gggtccattc cttgggcgtg acgcccgcgg cgtcgatgaa ctcctgctgg aactcgagca
     1981 cctcggcgat cgcgcgcttg gcggcctcga ggccttcggc gacggtctcc tcggtgggcg
     2041 cctgagcccc ggaggccagc agctcccagg tcttgtcggg cgcctcgccc tcgatcatca
     2101 ggatgtcgat ctcaccggag tcgctccggc ggccggccac gaccacgtcg aacgtggcct
     2161 cgtcggtctc ggcgaacgtg gggttgacgg tccactgccc gtcgatgagc gctacccgca
     2221 cggaggccac agggccgtcg aacggcaggc ccgccaggca ggtggcgacc gacgcggcgt
     2281 tcatcgccgg gatgtcgtag gggttgtccc cgtccgagcc caggaccgtg atgacgacct
     2341 ggacctcgtc gcggtagccg tcggcgaagg tcgggcgcag cggacggtcg atcagacggg
     2401 ccgtgaggat cgcggtctcg ccgggccggc cttcgcggcg gaagaagctg cccgggatct
     2461 tgccgatcgc gtacatccgc tcttcgacat cgatcgtgag cgggaagaag tcgatcccct
     2521 cgcgggggtt ggacgagacg cacgtgccga gcacctgggt ctcgccgtag gtgaccatga
     2581 ccgcgccgtc ggcgaggcgg gccatcttgc ccgtctcgaa ggtcaaggtc tggttcccga
     2641 tcttccggga aaggttggtg gtggccatgg ggccgttcct ccttgttcgt cttgaacgcg
     2701 caggcacttc ctggcgcgtc gatggagacg ggaggcgggt ccggccgatt ctcagtggtg
     2761 ggccaccggg atccttcaca ggtctcccgg gaactcgcta ctgataaccg accgcgggtc
     2821 gctcccacct ccggtggctt gcgatcagtg ggtcgtgcgc atcacttcct tcacgaacga
     2881 acgcctatcc agggcgtccc tccgatgtca tccgcgtcta tcggcgcagg ccgagacgcg
     2941 cgatcaacgc gcggtagcgc tcgatgtcct cgtgcatgag gtagtcgagc aaccggcgcc
     3001 gccgacccac catcttcagc aggccccggc gcgacgcgtg gtcgtgcttg tgggtcttga
     3061 ggtgctcggt gaggtccgcg atccgcttgg tcaggaccgc gatctgaacg tccgccgagc
     3121 cggtgtcgcc atctgcgcgg gcgtactccg tgatgatctg cgtcttctc
//
LOCUS       DATDBZ010000493         2880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_144744, whole genome shotgun sequence.
ACCESSION   DATDBZ010000493 DATDBZ010000000
VERSION     DATDBZ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144744"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VIX62_11570"
     CDS             complement(<1..731)
                     /locus_tag="VIX62_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860915.1"
                     /translation="MEPAERMALGMVDAERKRRQNVAGAEVLEIDGLVLAFSNLPDPQ
                     LNSVVVDHDPSDALGAMEAAEREFVRRSHPIGIDLQVGRNPGVDEAVRSMGLIRIIER
                     PGMALEVDALPEAPVPHGIEIRPVMSDEDVRGLVEVGVQAFDDDPEIGMSCYGVSSRG
                     VDGARAFIAWRGEEPVGIATGYLHEGAVGVMGVAVVPAARTLGLGSALTVQAARAFPE
                     GDLAWLHPSDEARSMYGRLGFRPVA"
     gene            767..1474
                     /locus_tag="VIX62_11575"
     CDS             767..1474
                     /locus_tag="VIX62_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEY5860916.1"
                     /translation="MKVGAVLVDFDGTAALHDVAEHLLVEFGDPAWPTYDEAVDRGEI
                     GLREAIVAQDLMLRADRETMLAFALEHCSMDPTFAPFVAWCREQDVPVTLVSDGFGFY
                     IEPLLTAAGIEGVGIITNEQIWSEDDRPSGMRFVSGHRECIGCGTCKMQAVLDAQRTR
                     GLVAFAGEGQTDRYGALYADVTFAKLELVAHCERDGVPYVPWNGFDDIRTWLESDAAL
                     PGPVAPVRCPGWTLPAI"
     gene            1453..2436
                     /locus_tag="VIX62_11580"
     CDS             1453..2436
                     /locus_tag="VIX62_11580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5860917.1"
                     /translation="MDTPSDLTELPDGLTARSMTLADVDIVTELVAACELHHDGVIEI
                     DREDIEVDWARPAYDLATESIGVFDADRLVAEAELFKGKRAEINVHPDSLGRGVGTAL
                     LGWTEDLAARKGSTRVSQTTTDANAAAAAILEAHGYGYGHTSWILEIEHDARPDDPSL
                     PAGIGFRDYESERDAHETYRVIEDAFNEWPNRDPATFEDWAALTIERPSFAPWQMILA
                     TDERTGDIVGVSFMLDYEAEGGWVQQVATRASHRHQGIARAMLVRAFQAFWDQGERVV
                     GLNTDSRTGALGMYEKVGMHVKRSYTNRAKDLGDDDSGGRRSDRARTGSEA"
     gene            2680..>2880
                     /locus_tag="VIX62_11585"
     CDS             2680..>2880
                     /locus_tag="VIX62_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860918.1"
                     /translation="MPPRELLRVLRGTRAGASGEIQIVPTAPHFVGAGLSHAGPWDYV
                     QRVPLLFYGPGIVPANGRVHGGA"
BASE COUNT          466 a          983 c          981 g          450 t
ORIGIN      
        1 tcggccacag ggcggaaccc caaccggccg tacatcgagc gcgcctcgtc gctggggtgc
       61 agccaggcga gatcgccctc ggggaacgcc cgcgccgcct gaaccgtcag cgccgatccc
      121 agaccgagtg tccgagcggc cgggacgacc gcgacaccca tgacccccac ggcgccctcg
      181 tgcaggtagc cggtcgcgat ccctacgggt tcctcgcccc tccacgcgat gaaggctcgc
      241 gcgccgtcca cccctcgtga actcaccccg tagcaggaca tgccgatctc gggatcgtca
      301 tcgaacgcct gcaccccgac ttcgaccagg ccccgaacgt cctcgtcgga catcaccggt
      361 cggatctcga tcccatgcgg gaccggcgcc tccggcaggg cgtcaacctc caacgccatc
      421 ccggggcgtt cgatgatccg gatcagtccc atcgaccgga cggcctcgtc caccccggga
      481 ttgcgaccaa cctgcaggtc gatcccgatc ggatgactcc gtcggacgaa ctcgcgctcg
      541 gccgcttcca tggcccccag cgcatccgac gggtcgtggt ccacgacgac ggagttcaac
      601 tgcgggtcgg gcaggttgga gaaggccagc accaatccat cgatctcgag cacctccgca
      661 ccggccacgt tctgacgccg cttgcgttcg gcatcgacca tgcccaacgc catccgttcc
      721 gctggttcca tccgcggagt gtggcaggcc gataggctcc cgcgacatga aggtgggggc
      781 ggtcttggtg gacttcgatg gcacggcggc gctgcacgat gtcgccgagc acctgttggt
      841 ggagttcggc gacccggcgt ggcccaccta cgacgaggcg gtcgaccgag gcgagatcgg
      901 ccttcgcgag gcgatcgtgg cgcaggacct gatgctgcgt gccgaccgcg agaccatgct
      961 ggcgttcgcg ttggagcatt gctcgatgga ccccacgttc gcgcccttcg tggcgtggtg
     1021 ccgtgagcag gacgttccgg tgaccctggt ctcggacggg ttcggcttct acatcgagcc
     1081 gctgctgacc gcggccggca tcgagggcgt gggcatcatc acgaacgaac agatatggag
     1141 cgaggatgat cggccctcgg ggatgcggtt cgtcagcggt caccgcgagt gcatcgggtg
     1201 cggcacgtgc aagatgcagg ccgtgctcga tgcccagcgc acacgcggtc tcgtggcgtt
     1261 cgccggcgag ggacagaccg atcggtacgg cgcgctgtac gccgatgtga cgttcgccaa
     1321 gctcgagctg gtcgcccact gcgagcgcga tggcgtgccg tacgtcccgt ggaacggctt
     1381 cgacgacatc cgcacgtggc tcgagtccga cgccgccctg ccgggtccgg tggcgccggt
     1441 acgctgtccc ggatggacac tcccagcgat ctgaccgagc tgcccgacgg cctcacggcc
     1501 cgctcgatga ccttggccga cgtcgatatc gtcaccgagc tcgtggctgc ttgcgagctc
     1561 caccacgacg gcgtgatcga gatcgatcgc gaggacatcg aggtcgactg ggcccgaccg
     1621 gcgtacgacc tcgcaaccga gagcatcggc gtgttcgatg ccgaccgctt ggtggccgag
     1681 gccgaactgt tcaagggcaa gcgtgccgag atcaacgtgc acccggattc gctgggtcgc
     1741 ggagtcggca ccgcgctgct gggttggacc gaggatctcg ccgcccgaaa gggttcgacg
     1801 agggtgtcgc agacgacgac ggacgccaac gccgcggccg cggccatcct ggaagcccac
     1861 ggctacgggt acggccacac ctcctggatc ctggagatcg aacacgacgc gcggccggac
     1921 gatccttccc tgcccgccgg gatcgggttc cgcgactacg agtccgagcg cgacgcgcac
     1981 gagacctacc gggtgatcga ggacgcgttc aacgaatggc cgaaccgtga tcccgccacg
     2041 ttcgaggact gggctgcgtt gacgatcgaa cgaccgagct tcgcgccgtg gcagatgatc
     2101 ctggcgaccg acgagcgcac cggggacatc gtcggtgtga gcttcatgct cgactacgag
     2161 gccgagggtg gttgggtcca acaggtcgcg acgcgcgcct cgcaccgcca ccagggcatc
     2221 gcccgcgcca tgctggtccg cgccttccag gcgttctggg atcaaggcga gcgcgtcgtc
     2281 gggctcaaca ccgattcacg aaccggcgcg ctggggatgt acgaaaaggt cggcatgcac
     2341 gtgaagcgca gttacacgaa ccgtgcgaag gaccttgggg acgacgacag cggggggcgg
     2401 cgctcggatc gagctcgcac gggcagtgaa gcctagcctg cgacgacgga tggcaggcat
     2461 ccatgcggga cccgggggct gctagcctcc tgggccgtga gttccgctgc ccgccgtctc
     2521 atacgtgtgt ttggagtgct cgtcgtcgtg gcggtcgttg cgaccatcgc cagcgcactc
     2581 ctggtggaca gaccagcccg gaccaccgac ctcacgtcga caccttcggt tgcgatacca
     2641 agagctatcg aacgtgccag cagcgagtca gcgtgcagtt tgcccccgcg tgagctgctg
     2701 cgggtgttgc gcggcacacg ggcaggggcg agcggcgaga tccagatcgt gccgaccgcg
     2761 ccgcacttcg tgggtgccgg cctcagccac gccggtccct gggactacgt ccagcgcgtt
     2821 ccgctcctgt tctatgggcc ggggatcgtg ccggcgaacg gtcgagtgca cggcggggcg
//
LOCUS       DATDBZ010000494         7649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2622695, whole genome shotgun sequence.
ACCESSION   DATDBZ010000494 DATDBZ010000000
VERSION     DATDBZ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2622695"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VIX62_11590"
     CDS             complement(<1..1418)
                     /locus_tag="VIX62_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5860919.1"
                     /translation="MSAHPTSERSLLGSSLVARYELRAVLGRGGMGEVYEAVDRQLNR
                     TVAVKILRPELAADRRFPARFRREARTVAGLSHPGIVAVYDVGEDDGNTFIVMELVAG
                     RTVGDLIESESPLDPGRVARIGMSAAQALAHAHSRGVVHRDVAPGNLMVTSGDEVKVL
                     DFGIARAARGSVRPSSGTTRGTVAYVAPEQLSGNTPDHRADIYGLGAVLYELATGRAP
                     FTGATAEEVAERARAEIPIAPRVLAGVPAGLSEAIQRCLAKDPADRPSDGGELAELLW
                     RAALASPPIERMPAPRAASTAETMPVPRAMTQVLSSNVAPVPVAGEVDPARVTAELNR
                     SSARRRIGRKVAIAALVSMAIGAALVAVPSIASMGSSVAATPKGPKPLAAPTGLTAAA
                     SCDGFMATGVDLAWSATGAGEGFEIWRRGGASAKPVLVGTITDPATTTFRDIDLGVDT
                     SYRYRVKAIDGPRVSRPSREAAV"
     gene            1533..2477
                     /locus_tag="VIX62_11595"
     CDS             1533..2477
                     /locus_tag="VIX62_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5860920.1"
                     /translation="MAGVIEIEGLRKEYRRLRGGRTIAVDGLDLEVPEGGVFGFLGPN
                     GAGKTTTIRCLLGLVAPSAGTLRLLGSEVPKGLPGVIGKVGAIVETPALFPRFSGRRN
                     LQILARIDGIGKATIEEVLTRVGLADRGDDAVRAYSLGMKQRLGIAAALMKDPQVLVL
                     DEPANGLDPAGIVEVRELVRSLGAEGRTVFISSHILSEIQHTADQVAILAKGRLIKAG
                     PVHEVLVAQRGEGVIVKTKDLQAGLTALTEAGLEVTATPDALRVAVAPEDAERISRTL
                     AERGLYVTELRPDEVDLETVFLELTADEPADPVPGGEP"
     gene            2474..3403
                     /locus_tag="VIX62_11600"
     CDS             2474..3403
                     /locus_tag="VIX62_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860921.1"
                     /translation="MIRLLSSEALRFRSRRMVKVLMILAIIGAVVGAVLAAAASSKPS
                     GDELAQAQSQRQAMIERCVRRDGFQDSFGPRPSGQSVQDYCSANIGVSEFIAGDPVTL
                     GEVGEYVKAAGFIVIVIGLVIGASMVGASWQTGTITTILTWEPRRIRWLLARLLSTAV
                     GVFIVTMVLLAVLAAALALASVVRGSTATEPGWLGDLLGTMARISVVASAIALIGAAV
                     ATIGRNTAAALGAVFVYLALLESLLRSLRPMLSRFMVGDSAVVLISGNELEVSDGSTL
                     MVLTQGHAYVVLATWVALVVAVGLVMLRARDVN"
     gene            3641..4141
                     /locus_tag="VIX62_11605"
     CDS             3641..4141
                     /locus_tag="VIX62_11605"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEY5860922.1"
                     /translation="MDFSTRTLSLIALVAIGLSLLAWVFAIAGSGGRRRTKQPSGPIP
                     MDDALRAVLEGHAAQIQRLEQAARVLNGTDKKQQVQIEGSVRHISLLRYDAFEDVGGR
                     LSFSCAMLDDHGTGVVLTSINGRQETRVYAKPIAEGNSTYNLSVEESEAIRQAMAPAG
                     EPVEAR"
     gene            4138..4644
                     /locus_tag="VIX62_11610"
     CDS             4138..4644
                     /locus_tag="VIX62_11610"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY5860923.1"
                     /translation="MIFRRDDDEADAVEATAPAPEATNPKRKGAEMADNDEVTIVGAG
                     ARLDGNLVSAGSLRIDGQVKGQINADGDVSLAPQSQVEADIRAQNVSVAGRFKGNIVV
                     KGKAHLARGGRIDGNITSKSLIVEEGGTFHGQSIMDASGSGSPAQADARAATGKGESK
                     DAEQVKVP"
     gene            4648..5931
                     /gene="serS"
                     /locus_tag="VIX62_11615"
     CDS             4648..5931
                     /gene="serS"
                     /locus_tag="VIX62_11615"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009189505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY5860924.1"
                     /translation="MLDIRAVREDPDRFRAGLARRDLAQAVDQLLAADERRRTLTTRV
                     DELRAEQNKASKAIGGAQGEDKQQLIAEVARVSAELKELEPQLAAAERELNALLAATP
                     NVPHESAPDGFTDEDAIEVRRHLEPTAFDFEPRDHAELGALLGVLDTERGARTSGSRF
                     VYLLGDLVFVQFALMRHAMDILADKGFAPVIPPVLVREEAMYGTGMLPADEAQLYSTT
                     QDDLYLVGTAEVPLAAFHQGEILDEADLPLRYAGYSSCFRREAGTYGKDMGGMFRVHQ
                     FDKVEMFSFATPESSWDEHEFLVGVEEEVLGNLEIPFRVVNVAAGDLGSSAAKKYDIE
                     GWLPGQDKYRELTSCSNTTDYQARRLQARVRRADGKVETLHTLNGTATAIGRTLIALL
                     ENHQQRDGSVQMPKALHPYLPERAKLLEPKVPSGS"
     gene            complement(5984..7564)
                     /locus_tag="VIX62_11620"
                     /pseudo
     CDS             complement(5984..7564)
                     /locus_tag="VIX62_11620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
BASE COUNT         1200 a         2584 c         2664 g         1201 t
ORIGIN      
        1 accgcggctt cgcgcgaggg ccggctcacg cgcgggccgt cgatcgcttt cacgcggtag
       61 cgatagctgg tgtcgacccc gagatcgatg tcgcggaacg tcgtcgtcgc cggatcggtg
      121 atggtcccga cgaggacggg cttcgcgctc gcgcctcccc ggcgccagat ctcgaagccc
      181 tcgccggccc cggtggcgct ccacgcgagg tcgaccccgg tggccatgaa gccgtcgcac
      241 gatgccgccg cggtcagccc ggtgggtgcc gcgagcggct tgggcccctt cggcgtggcc
      301 gcgacggacg agcccatcga cgcgatcgag ggcactgcca ccaaggcggc tccgatcgcc
      361 atcgacacca aggcggcgat cgccaccttc cgtcctatcc gacgtcgggc cgacgaacgg
      421 ttgagctcag ccgtcacgcg agcgggatcg acctccccgg cgaccgggac gggcgcgacg
      481 ttcgaggaca acacctgggt catcgctcgc ggcaccggca tggtctcggc ggtggaggcg
      541 gcacgaggcg ctggcatacg ctcgatggga ggggaggcga gggccgcccg ccacagcagc
      601 tcggcgagct cgccgccgtc ggatggacgg tcggcggggt ccttggccag gcatcgttgg
      661 atggcctccg acaggccggc cggaacgccg gccagcaccc ggggtgcgat cgggatctcc
      721 gcgcgcgcgc gttcggcgac ctcctcggcc gtcgctcccg tgaacggcgc cctgcccgtc
      781 gccagttcat acagcacggc gccgaggccg tagatgtcgg cgcggtggtc gggggtgttc
      841 ccgctcagtt gctcgggcgc gacgtaggcg accgtgcctc ttgtggtgcc cgagctcgga
      901 cggaccgagc cgcgtgcggc tcgcgcgatg ccgaagtcga gcaccttgac ctcatcgccc
      961 gaggtcacca tcaggttgcc gggcgcgacg tcgcgatgga ccacgccgcg tgagtgcgcg
     1021 tgggcgagcg cctgcgcggc gctcatgccg atgcgcgcca cccggccggg atccaacggc
     1081 gactcggact cgatcaagtc tccgacggtc cgacccgcga cgagctccat cacgatgaac
     1141 gtgttcccgt cgtcctcgcc cacgtcgtag accgccacga tcccggggtg cgacagtccg
     1201 gcgaccgtgc gggcttcccg gcggaaccgt gccgggaacc ggcggtcggc cgcgagttcg
     1261 ggacgcagga tcttcaccgc caccgtgcgg ttcagttggc ggtccacggc ctcgtagacc
     1321 tcgcccatgc cgcctcggcc caacacggca cggagttcat agcgagcgac gagcgaggag
     1381 cccaggagcg accgttcgga ggtcggatgc gcgctcatgt ggcgtccagc ctagcgtgag
     1441 ggctcccatg gcggcgaggt cgtcaccgca acgccacatg tcctggcgcc cacggcccca
     1501 acgggtccgg acgccggggt atggtgcgcc gcatggccgg cgtgatcgag atcgaagggc
     1561 tgcgcaagga gtaccggcgc ttgcggggcg gccggacgat cgccgtcgac ggtctcgacc
     1621 tcgaggtccc cgagggtggg gtgttcgggt tcctcggacc gaacggcgcc ggcaagacca
     1681 ccacgatccg gtgcctcctg gggttggtgg cgccgagcgc cggaaccctt cgcctgctgg
     1741 gcagcgaggt gccgaagggc ctgcccgggg tgatcggcaa ggtgggcgcc atcgtcgaga
     1801 cgccggcgct cttcccccgg ttcagcggca ggaggaacct ccagatcctc gcccgcatcg
     1861 acggcatcgg caaggccacg atcgaggagg tcctcaccag ggtggggctc gccgaccgtg
     1921 gggacgacgc cgtcagggcc tattcgctgg ggatgaagca gcgcctcggc atcgcggcgg
     1981 cgctgatgaa ggatccacag gtattggttc tggatgaacc ggcaaacggc ctcgaccccg
     2041 ccggcatcgt ggaggtgcgc gagctcgtgc gttctctcgg cgccgaggga cgcaccgtgt
     2101 tcatctcgag tcacatcctg tccgagatcc aacacaccgc cgatcaggtc gcgatcctcg
     2161 cgaagggtcg cttgatcaag gcaggcccgg tgcacgaggt gctcgtcgcg cagcgcggcg
     2221 aaggggtgat cgtcaagacc aaggatctgc aggcggggct caccgccctg accgaagcgg
     2281 gactcgaggt cacggcgacc cccgatgcgc tccgcgtcgc ggtcgcaccc gaggacgccg
     2341 agcggatctc gcgcacgctc gccgagcgcg gcctgtatgt gaccgaactg cgcccggacg
     2401 aagtggacct ggagaccgtc ttcctggagc tcaccgccga tgaacccgct gatccggtgc
     2461 cgggaggcga gccatgatcc gcctcctgtc ctccgaagcc ctgcggttcc gctcgcgcag
     2521 gatggtgaag gtcctgatga tcctggccat catcggagcc gtggtcggtg cggtcctggc
     2581 ggccgcggcc tccagcaaac cctcgggcga cgaactcgca caggcccagt cccagcgtca
     2641 ggccatgatc gagcgatgcg tgcgtaggga cgggttccag gactcgttcg gtcctcgacc
     2701 ttccgggcag tcggtgcagg actactgctc ggcgaacatc ggcgtgtcgg agttcatcgc
     2761 cggcgacccg gtgacgctcg gtgaggtcgg cgagtacgtg aaagccgccg ggttcatcgt
     2821 catcgtgatc gggctggtga tcggggcgtc gatggtcggc gcgtcgtggc agaccgggac
     2881 gatcaccacg atcctgacgt gggagccgcg acggatccgt tggttgctcg cccgcctgct
     2941 cagcacggcc gtgggcgtgt tcatcgtcac gatggtgttg ctcgcggtgc tcgccgccgc
     3001 ccttgccctg gcctccgtgg tccgtggatc gaccgcgacc gaaccagggt ggctcggcga
     3061 tctgctgggc acgatggcgc gcatctcggt ggtggcctcg gcgatcgcgt tgatcggcgc
     3121 cgcggtggcg acgatcggtc gcaacacggc ggcggcgttg ggagccgtct tcgtctacct
     3181 cgcgctgctg gagagcctgc tccgaagcct ccgtcccatg ctgagccgct tcatggtggg
     3241 cgacagcgcg gtggtgctga tctccggcaa cgagctggag gtctccgatg gctcgaccct
     3301 catggtgctg acccagggcc acgcctacgt ggtgctcgcg acctgggtcg ccctcgtggt
     3361 cgccgtgggc ctggtcatgc tgcgcgcgcg tgacgtgaac tgagcccgat cagccgtctc
     3421 atcgcctcaa gcgccgatgc tgcgccgccg atagcgcaaa ccacagcgat cggaacatga
     3481 acggagtctc atggtcgtgc gcatcaagcg cctgctgctc tcgctcggtt cggtggccgc
     3541 gttcctgatg gacgccggcg ccggccggcc caggtcctag cgttatactg cggattcctg
     3601 cggtcctggc cgggcaccgc gacggatcag caaggaaccg atggacttct ctacccggac
     3661 gctctcgctc atcgcactcg tcgcgatcgg actgtcgctg ctcgcgtggg tgttcgcgat
     3721 cgccggcagc ggcggccggc gtcgtacgaa gcaaccgtcc ggtcccatcc ccatggacga
     3781 tgccctgcgc gccgtgctcg agggccacgc ggcccagatc caacggctcg agcaggccgc
     3841 gcgcgtgctc aacggcaccg acaagaagca acaggtccag atcgaaggca gcgtgcggca
     3901 catctcgctg ctccgctacg acgcgttcga agacgtgggc gggcggctct cgttctcgtg
     3961 cgccatgctc gatgatcacg ggacgggtgt ggtgctcacg tcgatcaacg gacgacagga
     4021 gaccagggtg tacgccaagc ccatcgccga ggggaacagc acctacaacc tctcggtcga
     4081 agaatccgaa gcgatccgcc aggcgatggc gccggcgggc gaaccggtgg aggcgcgatg
     4141 atcttcaggc gggacgacga cgaagcggac gcggtcgaag ccacggctcc ggccccggaa
     4201 gccacgaacc cgaaacggaa gggagcggag atggcggaca acgatgaggt cacgatcgtc
     4261 ggggccggtg cgcggttgga tggcaacctg gtgtcggcgg gttccctccg gatcgatggt
     4321 caggtgaagg gtcagatcaa cgccgacggc gacgtctcct tggcaccgca gagccaggtc
     4381 gaagccgaca tccgcgcgca gaacgtcagc gtggccggcc ggttcaaggg caacatcgtc
     4441 gtcaagggca aagcgcacct ggcccgcggc ggccgcatcg acggcaacat cacgtccaag
     4501 tcgctcatcg tcgaggaagg cggaaccttc cacggccaga gcatcatgga cgcctcgggg
     4561 tccggatcgc ccgcccaggc cgacgcgcgc gccgcgaccg gcaagggcga gtcgaaggac
     4621 gccgagcaag tgaaggtgcc gtgacccatg ctggacatcc gtgccgtccg ggaggacccg
     4681 gaccgattcc gtgccgggct ggcccggcgc gacctggcac aagcggtcga ccagttgctg
     4741 gccgccgacg agcgtcgcag gaccctcacc acccgcgtcg acgagctacg agccgaacag
     4801 aacaaggcgt cgaaggcgat cggcggcgcg cagggtgagg acaagcagca gctgatcgcc
     4861 gaggtggccc gcgtgtcggc cgagctcaag gagctcgaac cgcagctcgc ggcggccgaa
     4921 cgcgaactca acgcgctgct cgccgccacg ccgaacgtgc cgcacgagag cgcgcccgac
     4981 gggttcaccg acgaggacgc catcgaggtg cgccgacacc tcgagcccac cgcgttcgac
     5041 ttcgagccgc gcgaccacgc ggagctcggg gcgttgctgg gcgtcctcga caccgagcgt
     5101 ggtgcgcgaa cgagcggatc acggttcgtc tacctgctgg gcgatctggt gttcgtgcag
     5161 ttcgcgttga tgcggcacgc gatggacatc ttggccgaca aagggttcgc tccggtgatc
     5221 cctcccgtgc tcgtgcgcga ggaagcgatg tacggaaccg gcatgctgcc ggccgatgag
     5281 gcgcagctct actccacgac ccaggacgat ctctatctcg tgggcacagc tgaggtgccg
     5341 ctcgcggcgt tccaccaggg tgagatcctc gacgaggcag acctgcccct gcgctacgcc
     5401 gggtactcat cgtgcttccg tcgcgaggcg ggcacgtacg gcaaggacat gggcggcatg
     5461 ttccgcgtgc accagttcga caaggtggag atgttcagct tcgcgacgcc cgagtccagc
     5521 tgggacgaac acgagttcct cgtgggcgtc gaagaagagg tgctgggcaa cctcgagatc
     5581 cccttcaggg tggtgaacgt cgccgcgggc gatctcggga gcagcgcggc gaagaagtac
     5641 gacatcgagg gatggctgcc cggccaggac aagtatcgcg agctcacctc atgctcgaac
     5701 accaccgact accaggcgcg gcggctccag gcgcgggttc gccgggcgga cggcaaggtg
     5761 gagacgctcc acaccctgaa cggcaccgcc accgccatcg gccggacgct catcgcgttg
     5821 ttggagaatc accagcagcg ggacggttcg gtgcagatgc cgaaggcgtt gcatccgtac
     5881 cttcccgagc gcgcgaagct gttggagccg aaggtgccat ctggctcgtg acgtcgaacg
     5941 tcacgccctg ccatcgctgc aacggttcgt cggggtccga gaccacctgc gccatgccgt
     6001 ggagccatcg cacctcgccg tcgcgacgga tgacacggaa caccaggtcc cagggttcac
     6061 cgctcctgtc ggagcgctcc gtcaccttga tcgcatgggc ccggtcgtcc ggatggagca
     6121 tgcgctcgaa atgccgtggt tcggcgatca gttcctcggg cgtgtagccg agcatctcac
     6181 tgctctgcac gcccatgaac tcgtagcgcg aggcccccgt cagcgggtcg atgacctcgg
     6241 tccacggaag cgcaggcatc ccgtcgagca ccccgagagc ctgttccgct gaccggcgga
     6301 gccgctcctc ttcgcgcttg cggtcgtcga tgtccaagat gacgccctga tagccggtgg
     6361 gcagtccgtc gtcgccgaaa ccgacggcgc ggccttccac gtggaaccag atcacacgtc
     6421 cgtcggcgtg gatgaaccgg aaatcgctgt tccacggctc gccggttcgc cagatgtcct
     6481 ccgtttcggc aaccacccgc gccttgtcgt cggggtgcag gaaggcgaac catccctcgg
     6541 gtggtgccga gaaggcgtcg ggtggatacc cggactgctc gaggctgcga ggactcacgt
     6601 agtggtgacg ccaccgcacg gcgggatcgg ggtcgcggtc ggacacgacc cacatgatcc
     6661 cggggttctc ctcggtgagc cgcttgaacc acgcttcggc ttcggaggcc ttcgtctcgg
     6721 cctccttgcg tgcggtgatg tcgagcaaga ccccctggaa caggcggggg cggccacgct
     6781 cgtcgcgtgt ccgcagggtc gcgcggtccc agacccacac caccccacca tccttcgcga
     6841 ggtagcggta ctcgagattg agcggcacgc cggaagcgat gcaccggtcc gcctcggcat
     6901 ccacccggtc gcgatcgtgc ggatgcaccc gttgacgcca gaatcccgga cgcatccatt
     6961 cgtccgccgt gaagcccagc atgtcggcga tggcaggact cgtgtaggcg gcatgttctt
     7021 ccgggagtgg gtccccgtcg agccagttca tctcccatga atagacgacg gcgggcaggt
     7081 cctcgacgag catgcggtag cgggtctcgg cttcgcggag ttcggcttcg acgcgacgcc
     7141 gctcgccgat ctccaagctc atcatctccg cggcgtcctc gagctcggcg gtccgtgcca
     7201 gcacgcgcca ctccagatcg tcgttggcgg cctggaggct ctgctcccgg tctttctccg
     7261 ccgtgatgtc gagcatcacg ccctgccaac gagcgtcgat cgtcactggg tcctcgacga
     7321 gcacggcttg atcacggatc cacacgatcc gtccgtcgtt ggcgatcagc cggtactcgc
     7381 ggcgccacgg ttcgccggtt tcgttgtgca ggtcgtgggc ggccagctcg atctcgcggt
     7441 cgtcgggatg cagcagctcg gtccagatgt cgggttgatc gagccactct tggcgcgagt
     7501 acccgaggat gtcttcgacg tggggactca cgaaggtggt gcgacgctcg tcgtcggggc
     7561 ccatctcgta gaccaccgcg ggaacctgtt cgacgagggc ccggtagcgc cgctccgatt
     7621 cgcgaaggtc gtgctcggcc ttcttcgcg
//
LOCUS       DATDBZ010000495         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_986684, whole genome shotgun sequence.
ACCESSION   DATDBZ010000495 DATDBZ010000000
VERSION     DATDBZ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986684"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VIX62_11625"
     CDS             <1..1003
                     /locus_tag="VIX62_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5860925.1"
                     /translation="ALSGGPSSGALVAGNTVVLKPSNQGALLGYKLYECYRDGGVPAG
                     AFHLVTGRGAVAGDHLWHHPDVNGITFTGSYPVGMDIYKHFSTDVPKPVICEMGGKNP
                     TIVTKNADLDKATDGVLRSAFGFGGQKCSACSRVYVEREVYDDFVTQLKTKTEKIKVG
                     NPIERDVYMGPIINEPAMETYEEASEEARKNGTIVTGGQRITEGDFGRGLFVEPTIVE
                     APEDSWLWKKELFVPFVAVAPFDELDDAIDKANDTEYGLTSGFFSEDRAEIDEWLDRV
                     QAGVVYVNRRAGSTTGAWPGVQPFGGWKGSGTTGKAGGGPYYVTQYLREQSRTVIEE"
     gene            1000..2310
                     /locus_tag="VIX62_11630"
     CDS             1000..2310
                     /locus_tag="VIX62_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5860926.1"
                     /translation="MTATERDLTFDALAAHSGPKLITDIPGPESLARIARDGKVTSPS
                     LPRAYPFVPKRGAGSIVEDVDGNLFLDINAGIAVTSTGHCHPRVVEAIQRQATQLLHY
                     SASDFYLPIYSDVAERLAATAPFGGAPARVFLSNSGTEAVEAAIKLARYTTGRQYLIG
                     FYQSFHGRSMGSVTLTASKAKYRQNFGPMLPSVYHSFYGDFDYLEEVLFKRVVTPEEV
                     AAIVVESWLGEGGYVLPPEGWFRYLRELCDRHGIMLVCDEVQSGVGRSGTMWAIEQEG
                     VTPDIITAGKGIASGLPLGAMIARDEVMTWEAGAHGSTYGGSPVSCAAALATLDVIED
                     EGLMANASAVGEVFLTGMREIAQRHPIITEVRGRALWIGLSFADHDQATAVEQASFRR
                     GLLALGCGDDAIRISPPLIFREDQARIALEIFEEAVADVESAGP"
     gene            2335..>2523
                     /locus_tag="VIX62_11635"
     CDS             2335..>2523
                     /locus_tag="VIX62_11635"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5860927.1"
                     /translation="MSASIDTLTFDAVDPLRLAAFWEAALGFEVADGADDEGAYLADP
                     SGRTRGVFFQRVPEPKVAK"
BASE COUNT          420 a          831 c          879 g          393 t
ORIGIN      
        1 ggcgctgtcg ggcggaccgt ccagcggggc gttggtggcc ggcaacacgg tcgtgctgaa
       61 gccctcgaac cagggcgcgc tcctgggcta caagctctac gagtgctatc gcgacggcgg
      121 cgtgccggcg ggcgccttcc acctggtgac gggacgcggc gcggtggccg gcgaccacct
      181 gtggcaccac cccgacgtga acggcatcac cttcaccggg tcctacccgg tgggcatgga
      241 catctacaag cacttctcca ccgacgtgcc gaagccggtg atctgcgaga tgggtggcaa
      301 gaaccccacg atcgtcacca agaacgcgga tctggacaag gccaccgacg gagtgttgcg
      361 cagcgccttc gggttcggcg ggcagaagtg ctcggcctgc agccgggtct acgtggagcg
      421 cgaggtctac gacgacttcg tcacccagct caagaccaag accgagaaga tcaaggtcgg
      481 caacccgatc gagcgcgacg tctacatggg tccgatcatc aacgagcccg cgatggagac
      541 ctacgaagaa gcctccgagg aggctcgcaa gaacggcacg atcgtcaccg gcggccagcg
      601 catcaccgaa ggggacttcg gtcgagggct gttcgtggag cccacgatcg tcgaggctcc
      661 cgaggacagt tggctgtgga agaaggagct gttcgtgccg ttcgtggcgg tggcgccgtt
      721 cgacgaactc gacgacgcga tcgacaaggc gaacgacacc gagtacgggt tgacctccgg
      781 gttcttcagc gaggaccggg ccgagatcga cgagtggctc gaccgtgtgc aggcgggcgt
      841 cgtgtacgtg aaccggcgcg cgggctccac caccggcgca tggcccggtg tgcagccctt
      901 cggcggctgg aagggctcgg gtaccaccgg caaggcgggt ggagggcctt actacgtcac
      961 ccaatacctg cgcgagcagt cgcggacggt gatcgaggag tgaccgccac cgagcgcgat
     1021 ctcacgttcg atgctctcgc cgcccacagc ggcccgaaac tgatcaccga catccccggt
     1081 cccgagtcgc tcgcgcggat cgcgcgcgac ggcaaggtca cgtcgccctc gctgccgcgg
     1141 gcctatccgt tcgtgcccaa gcgcggcgcg ggatcgatcg tcgaagacgt cgacggcaac
     1201 ctgttcctgg acatcaacgc cggcatcgcg gtgacgtcca cgggccattg ccatccgagg
     1261 gtggtcgagg cgatccaacg gcaggccacg cagctgctgc actactcggc gagcgacttc
     1321 tacctaccga tctactccga cgtcgccgaa cgactggccg ccaccgcgcc cttcggcggt
     1381 gcgcccgcgc gcgtgttcct gtccaactcg ggcaccgagg cggtggaggc ggcgatcaag
     1441 ctcgcccgct acaccaccgg ccggcagtac ctgatcggct tctaccagtc cttccacggg
     1501 cggtcgatgg ggtcggtcac gctcaccgcc tcgaaggcga agtaccgcca gaacttcggc
     1561 cccatgctgc ccagcgtcta ccactcgttc tacggcgact tcgattacct cgaagaggtg
     1621 ttgttcaagc gggtcgtcac ccccgaagag gtcgcggcga tcgtcgtgga gtcctggttg
     1681 ggcgaagggg gctacgtgct cccgcccgag gggtggttcc gctacctgcg ggagctctgc
     1741 gaccgtcacg gcatcatgct cgtctgcgac gaggtccagt cgggcgtcgg acggagcggc
     1801 acgatgtggg cgatcgagca ggagggggtt acgcccgaca tcatcaccgc cggcaagggc
     1861 atcgcgagcg gcctgccgct cggcgcgatg atcgcccgcg acgaggtgat gacgtgggag
     1921 gccggtgcac acggttccac gtacggcggt tcgccggtct cgtgcgccgc ggcgttggcc
     1981 acgctcgacg tgatcgagga cgaaggactg atggcgaacg cgtccgcggt gggcgaggtc
     2041 ttcctcacag gcatgcgcga gatcgcgcag cgccatccca tcatcacgga ggtgcgcggc
     2101 cgggccctgt ggatcgggct ctcgttcgcc gaccacgatc aggcgaccgc agtggagcag
     2161 gcttcgttcc ggcgcggcct gctcgcgttg gggtgcggcg acgacgcgat ccgcatctcg
     2221 ccgccgctga tcttccgcga ggatcaagcc cgtatcgcgc tggagatctt cgaagaggcc
     2281 gtcgccgacg tggagtctgc cggtccgtag tccggccgca ggacgatgcc gagcgtgagc
     2341 gcttcgatcg acacgctgac cttcgatgcc gtcgatccgc tgcggttggc ggcattctgg
     2401 gaggcggcgc tcgggttcga ggtggccgac ggggccgacg acgaaggcgc ctatctcgcc
     2461 gacccgtccg gcaggacgag gggggtgttc ttccaacgag tgcccgaacc caaggtcgcc
     2521 aag
//
LOCUS       DATDBZ010000496         6956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_3023435, whole genome shotgun sequence.
ACCESSION   DATDBZ010000496 DATDBZ010000000
VERSION     DATDBZ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3023435"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VIX62_11640"
     CDS             <1..1187
                     /locus_tag="VIX62_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5860928.1"
                     /translation="SVMGAAVVRAYGLDDTIHARVTGAIGRRYDAEVKAHLRAATLWP
                     MSSVFYALTLSVVVAVGALFGPEWGLTFGTVTAFLFLGDVFLGVFEDLPEIYAETQTA
                     IAGWRKILAVLDLPVEIEEPTDGVRLPTHGALDVAVHDMTYAYRNGKPVLHDIDVTVP
                     AGSHIAIVGETGSGKSTFAKLLTRLADPADGLIEVGGVDLREIDPSSRRVAIRMVPQD
                     GFLFDWTVRENVKVGRDGATDREVDAAFEELGLGDWVRALQDGLDTRAGERGEQLSVG
                     ERQLIALVRAQIADPGLLILDEATSAVDPATEARITEALRRLSAGRTTVTIAHRLSTA
                     EHADRVLVFDAGRIVEEGTHDELIAGRGVYAGLFQSWLGNTQVDSADPQQAEVQPDPS
                     AR"
     gene            1199..1459
                     /locus_tag="VIX62_11645"
     CDS             1199..1459
                     /locus_tag="VIX62_11645"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEY5860929.1"
                     /translation="MPESKGRQKKKSPRYRAPSQQAKAKHKSSPRWYGPLLITLMAAG
                     VLVIVLNYVGLIPGTDNQADPIYLWVGLGLIGVGFVGTTFWY"
     gene            complement(1629..1886)
                     /locus_tag="VIX62_11650"
     CDS             complement(1629..1886)
                     /locus_tag="VIX62_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860930.1"
                     /translation="MPTWLLVLTAILIGALIWRIGIASIRSFSPDPTKDVAPDEPEDV
                     EDLDVYLVCGECGTEYKVSRLGELQVPRHCGERMQVVRRPA"
     gene            complement(1905..3602)
                     /locus_tag="VIX62_11655"
     CDS             complement(1905..3602)
                     /locus_tag="VIX62_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007384990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY5860931.1"
                     /translation="MDRPRAALSSRAGRAGLLAIPLAFLALFFVFPLVSILDRGLRPD
                     GALDLGAIGRVIGDPDLRGVAWFTLWQATVSTVLTLLVGLPAAFVLSRFAFRGRRVVR
                     AMTLVPFVLPTVVVGTAFVGLLGPGSPGATAAGWVGIDAGDGLHRTIGALLAAHVFFN
                     IAVVVRVVGSYWDQVDPEIEETAESLGAGRIETFRRVLLPIARPAIASAAALVFLFAF
                     TSFGIVLLLGRPGGSTLEVEVYRHTSQLLDLSTAAVLALVQMVFVGALLLVESVLAAR
                     AGVPLSLVTTGAARPIRDRRERLLVVSVVGATCVLLVTPVLALVWRAFGGGLGELSLR
                     AFTTLGDARRGSVLSVAPLSSIGHSLVTATAAAVFAVVIGVPAALAIARARRSSWLTV
                     LVALPLGVSAVTVGFGYVVAFDDAPLDLRGSAWVVPLAQAVIALPFVVRIVTPVLVSI
                     ESGLTEAAADLGASPGRILTAVTLPIARPAVAAAGAFAFIVALGEFGAAAFLATPDRP
                     TLPVAISRLLGQPGSASLSQAAAMSVILMVVTAVGALAIDRFGRGTGGAQVATGRTM"
     gene            complement(3578..4633)
                     /locus_tag="VIX62_11660"
     CDS             complement(3578..4633)
                     /locus_tag="VIX62_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5860932.1"
                     /translation="MAVFAVSALAAACTSSDPPNEATGSASSEPVTLTLLTHDSFDAS
                     EKVLAAFTDETGIDVRVVPAGDAGQLVNRAILSAGNPEGDVLFGIDDNLLAGALDAGV
                     FASYTSPRLDQVDDAYDVDPEHRVTPIDHGEVCLNVDIGWFDDHGLPAPTSLSDLTDP
                     AYRGLTVVENPATSTPGLAFLLATVAEFGEQGWETYWTALRDNDVLVVDGWEGAYYGE
                     FSGAGGGEGTHPIVVSYASSPVAEVVFADPPVDAAPTAVVASSCFRQIEFAGVLEGTD
                     HPAEAGRLIDFLLSRPFQEDIPLKMFVFPVAEDATLPPEFVRYAVVPDSPLTLSPELI
                     ADRRAAWVDRWTDLVLR"
     gene            complement(4653..5306)
                     /locus_tag="VIX62_11665"
     CDS             complement(4653..5306)
                     /locus_tag="VIX62_11665"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEY5860933.1"
                     /translation="MASPRTALVVCGGGPVLAPGALPAAPDLVIAADDGAREALRLGL
                     EVDVVVGDMDSVPASTLAQVERAGGRVMRHPADKDATDLELALDEALTSQAERILVAG
                     GAGGRLDFVIANALALGHPRLAAVEVDAVFGVARLHVVRSERALDGSPGEVLSLLAVG
                     GPAAGVRTRGLRWTLDGQDLPAASGWGVSNEFAMAEASVSIEAGVVLVVRPGEVRDA"
     regulatory      complement(5293..5406)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5446..6807)
                     /gene="gdhA"
                     /locus_tag="VIX62_11670"
     CDS             complement(5446..6807)
                     /gene="gdhA"
                     /locus_tag="VIX62_11670"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEY5860934.1"
                     /translation="MSEYVRRLMAEVTAKNPNEPEFLQAVQEVTDSLAPVLERHPKYR
                     EGAILERLIEPERVIMFRVPWQDDDGEVHVNRGIRVEMNSAIGPYKGGLRFHPTVYLG
                     MLKFLAFEQIFKNALTTLPIGAGKGGADFDPKGKSDAEVMRFCQSFMTELQRHIGPHT
                     DVPAGDIGVGGREIGYLFGQYKRIRNEFTGSLTGKGLDWGGSLIRPEATGYGAVYFAE
                     QMLHTRGDTLDGKVCLVSGSGNVAQYTVEKLLDFGAKPITLSDSGGVVYDKNGIDRDK
                     LHWVMDLKEERRGRIMEYADRFPGAEFIPADPRNDHNPLWDIAADCAFPSATQNEINA
                     KDAANLLNNGVGVVSEGANMPTVPEGVEAFVSASILYGPGKAANGGGVAVSGLEMSQN
                     SMRVTWSREEMDQRLYSIVTRIHEQCRDTAEDYGHPGNYVMGANIAGFVKVADAMLSQ
                     GLI"
BASE COUNT         1144 a         2543 c         2327 g          942 t
ORIGIN      
        1 agagcgtgat gggcgccgcg gtggtccgcg cctacggcct ggacgacacg atccacgccc
       61 gggtgaccgg tgcgatcggt cgccggtacg acgccgaggt gaaagcccac ctgcgtgcgg
      121 cgaccctgtg gccgatgtcc agcgtcttct acgccctcac gctctcggtc gtggtggcgg
      181 tgggcgcgct gttcgggccg gagtgggggc tgacgttcgg cacggtcacc gcgttcctgt
      241 tcctgggcga cgtgttcctg ggggtgttcg aggacctgcc ggagatctac gccgagaccc
      301 agaccgcgat cgcgggctgg cgcaagatcc tggccgtgct cgacctgccg gtggagatcg
      361 aagagcccac cgacggtgtg cgcctgccca cgcacggcgc cctcgacgtg gccgtgcacg
      421 acatgaccta cgcctaccgc aacggcaagc ccgtgctgca cgacatcgac gtcacggtgc
      481 cggccggttc gcacatcgcg atcgtgggag agaccggcag cggcaagtcg acgttcgcca
      541 agctgctgac ccggctcgcc gaccccgccg acggcctgat cgaggtgggc ggcgtcgacc
      601 tgcgcgagat cgatccttca tctcgccgcg tcgcgatccg catggtgccg caggacgggt
      661 tcctgttcga ctggaccgtg cgggagaacg tgaaggtcgg ccgcgacggc gccaccgatc
      721 gcgaggtcga cgccgccttc gaggagctgg gactgggcga ctgggtccgc gcgctgcagg
      781 acggcctcga caccagggcg ggagagcgag gtgaacagct ctcggtgggt gaacggcagc
      841 tgatcgcgct ggtacgcgcc cagatcgccg acccgggact gctgatcctg gacgaggcga
      901 cgagcgcggt ggaccccgcc accgaggcgc ggatcaccga ggccctgcgc cggctctctg
      961 ccggccggac caccgtcacg atcgcacacc gcttgagcac cgccgagcac gccgaccgcg
     1021 tgctggtgtt cgacgccggc cggatcgtgg aggagggcac ccacgacgaa ctcatcgccg
     1081 ggcgcggcgt gtacgccggg ctgttccaga gctggctggg caacacccag gtcgacagcg
     1141 cagatcccca gcaggccgag gtgcaaccgg acccgtcggc ccggtagcat ctcgggcgat
     1201 gccggagtcc aaaggtcgcc agaagaagaa gtcgccgcgc taccgcgcgc cttcccagca
     1261 ggccaaggcc aagcacaagt ccagcccacg ctggtacggc ccgctactga tcacgctgat
     1321 ggccgccgga gtcctggtca tcgtcctcaa ctacgtggga ttgatcccgg gtaccgacaa
     1381 ccaggccgat ccgatctacc tgtgggtggg actcggcctg atcggcgtcg gcttcgtggg
     1441 caccaccttc tggtactgag ccgacctccc ggttcaaggc ctcggctgaa tcacaccgat
     1501 gtgatcttcc acaggcttgt ccacaccggt ggataaccac ctgcgtggtc gcccggcgtt
     1561 cgtcggaggt tcatctcctg tggataaccg gtggataccc gtgggggacc cggtggataa
     1621 ccgccggatc aggccggccg gcggaccacc tgcatgcgct caccgcagtg ccgagggacc
     1681 tggagttcgc ccagccgcga caccttgtat tccgtgccgc actcgccaca caccaggtag
     1741 acgtcgagat cctcgacgtc ctcgggctcg tcgggcgcca cgtccttcgt ggggtcgggc
     1801 gagaacgagc ggatcgacgc gatgccgatc cgccagatca gcgcgccgat caggatcgcc
     1861 gtgaggacga gcagccaggt gggcacggtt ccacgctaac agcctcacat cgtgcgaccg
     1921 gtcgcgacct gggcaccacc cgtaccgcgt ccgaagcggt cgatcgccag cgcccccacg
     1981 gccgtcacca ccatgaggat cacgctcatc gcggccgcct ggctcaggga cgccgaaccc
     2041 ggctggccca gcagccgcga gatcgccacc ggcagcgtcg gccggtccgg ggtggccagg
     2101 aacgccgccg ccccgaactc ccccaacgcc acgatgaagg cgaacgctcc ggcggccgcg
     2161 accgcgggcc gggcgatcgg cagcgtcacg gccgtcagga tccggcccgg cgaggcaccg
     2221 aggtcggccg ccgcctcggt gagcccggac tcgatcgaga ccagcaccgg cgtcacgatc
     2281 cggaccacga acggcagggc gatcaccgcc tgggccagcg gcaccaccca ggccgaaccc
     2341 cggaggtcga gcggcgcgtc gtcgaaagcg accacgtagc cgaagcccac ggtcaccgcc
     2401 gagacaccca acgggagggc caccagcacg gtgagccacg atgatcgccg cgctcgggcg
     2461 atcgccagcg ccgccgggac gccgatcacc acggcgaaca cggccgcagc cgtcgccgtc
     2521 acgagcgaat ggccgatcga gctcagcggc gcgaccgaca gcacgctccc ccgccgcgcg
     2581 tcgccgagcg tcgtgaacgc ccggagcgac aactcaccga gacccccgcc gaacgctcgc
     2641 cacacgaggg cgaggacggg cgtgaccagc agcacgcagg tcgcgcccac caccgagacc
     2701 accagcagcc gctcgcgccg gtcccggatc ggccgggccg cacccgtcgt gaccagcgac
     2761 agcggtaccc cggcgcgcgc cgccagcacg ctctccacca acaggagcgc gccgacgaac
     2821 accatctgca ccagcgccaa caccgcggcc gtcgacagat cgagcagttg cgaggtgtgc
     2881 cggtagacct cgacctcgag cgtcgaaccg cccgggcgtc ccaacagcag cacgatgccg
     2941 aacgacgtga acgcgaacag gaacaccaac gccgcggcgc tcgcgatcgc cgggcgggcg
     3001 atcggcagca acacccgccg gaacgtctcg atgcgacccg cccccaacga ctcggcggtc
     3061 tcctcgatct cgggatccac ctggtcccag tacgagccaa ccacgcgcac gaccacggcg
     3121 atgttgaaga acacgtgcgc ggccagcagc gctccgatcg tgcggtgcag cccgtcgccc
     3181 gcatcgatcc cgacccatcc ggccgcggtc gcccccgggc tccccggtcc gagcagcccg
     3241 acgaacgccg ttcccaccac gacggtgggc agcacgaacg gcaccagggt catcgctcgc
     3301 accacgcgac ggccacggaa ggcgaaccgc gacaggacga acgccgcggg cagccccacg
     3361 agcaacgtca gcaccgtgga gacggtcgcc tgccacagcg tgaaccacgc cacccctcga
     3421 agatcggggt cgccgatcac ccgtccgatc gcgcccaggt cgagcgcgcc gtcgggccgg
     3481 agcccacgat cgaggatcga gaccagcggg aacacgaaga agagcgcgag gaacgccagg
     3541 gggatcgcca gcagccccgc gcgtcccgcc cgggagctca gcgcagcacg aggtcggtcc
     3601 accggtccac ccacgccgcc cgtcggtccg cgatcagctc cggcgacaag gtcagcggcg
     3661 agtccggcac gacggcgtac cggacgaact cgggcggcag cgtcgcatcc tcggccaccg
     3721 ggaacacgaa catcttcagc gggatgtcct cctggaacgg ccgcgacaac aggaagtcga
     3781 tcaaccggcc ggcttctgcg ggatggtcgg tcccctcgag gacgccggcg aactcgatct
     3841 ggcggaaaca gctcgaggcc accaccgccg tcggcgcggc atcgaccggc ggatcggcga
     3901 acaccacctc ggccaccggg ctcgaggcgt acgacaccac gatcggatgg gtcccctcac
     3961 cgccgccggc ccctgagaac tcgccgtagt aggcgccctc ccagccgtcg acgacgagca
     4021 cgtcgttgtc gcggagcgcc gtccagtagg tctcccaccc ctgttcaccg aactcggcga
     4081 cggtggccag caggaacgcg agcccgggcg tcgaggtggc cgggttctcc acgaccgtga
     4141 gcccgcgata ggccgggtcg gtcagatcgg acagcgacgt cggcgccggc aggccgtggt
     4201 cgtcgaacca tccgatgtcg acgttgaggc acacctcgcc gtggtcgatc ggcgtcaccc
     4261 ggtgctcggg atcgacgtcg taggcgtcgt ccacctggtc cagcctcggt gaggtgtagg
     4321 acgcgaacac ccccgcgtcc agcgcgccgg ccagcaggtt gtcgtcgatg ccgaagagca
     4381 cgtcgccttc cgggttgccg gccgacagga tcgcgcggtt cacgagctga ccggcgtcac
     4441 cggccggcac cacccgcacg tcgatgcccg tctcgtcggt gaaggcggcg agcaccttct
     4501 cggatgcgtc gaacgaatcg tgggtcagca gcgtgagggt caccggctcg gaggatgcgg
     4561 aaccggtggc ctcgttcggg ggatcggagg aggtacacgc ggcggccagg gcgctcacgg
     4621 cgaacacagc cacgaaggca gcccaccgac gcctatgcat cgcgcacctc accgggccgg
     4681 accacgagga cgaccccggc ctcgatcgag accgaggcct ccgccatggc gaactcgttg
     4741 ctgacgcccc agcccgatgc ggccggcagg tcctggccgt ccaaggtcca gcgcagcccg
     4801 cgggtccgca cgccggcggc cggaccaccg acggcgagca ggctcaggac ctcgccgggg
     4861 ctaccgtcca gcgcacgctc actgcgcacg acgtggagac gggcgacccc gaagacggcg
     4921 tcgacctcca ccgcggcaag ccgtggatgc cccagcgcca gcgcgttcgc gatgacgaag
     4981 tccagccgcc ccccggctcc cccagcgacc aggatccgct ccgcctgtga ggtgagcgct
     5041 tcgtcgaggg ccagctccag atcggtggcg tccttgtcgg cgggatggcg catcacccgt
     5101 ccccccgctc gctccacctg ggcgagcgtc gaggccggga ccgagtccat gtcgcccacc
     5161 acgacgtcga cctccaggcc gaggcgcaac gcttcgcggg cgccgtcgtc ggccgcgatg
     5221 acgaggtccg gggccgcggg caaggccccc ggtgcgagga cgggcccgcc gccacagacg
     5281 acgagcgcgg tgcggggtga cgccatcagc tccctccgcc agcatgacct ggttcaggtt
     5341 cgtcgggtcg ccgacggggt agtcggcctc tcagcccggt ccttccgggc tccccggtga
     5401 ttccctccca gtctacggcg gcgcgcgacg gatggcgcgg cggcgctaga tcaggccctg
     5461 cgagagcatc gcgtcggcca ccttgacgaa gccggcgatg ttggcgccca tcacataatt
     5521 gccggggtgg ccgtagtcct cggcggtgtc acggcactgc tcgtggatgc gcgtcacgat
     5581 cgaatagagc cgctggtcca tctcctcgcg cgaccacgtc acgcgcatcg agttctggct
     5641 catctccagg ccgctcacgg ccacaccgcc cccgttcgcg gccttgcccg ggccgtacag
     5701 gatcgaggcc gacacgaacg cctccacgcc ctctggcacc gtgggcatgt tcgcgccctc
     5761 gctcaccaca ccgacgccgt tgttcagcag gttcgcggcg tccttggcgt tgatctcgtt
     5821 ctgggtggcg ctggggaacg cgcagtcggc ggcgatgtcc cacagcgggt tgtggtcgtt
     5881 ccgcgggtcg gcggggatga actcggcccc ggggaaccgg tcggcgtact ccatgatgcg
     5941 tccgcggcgc tcttccttga ggtccatcac ccagtggagc ttgtcgcgat cgatcccgtt
     6001 cttgtcgtac accacaccgc ccgagtccga cagcgtgatc ggcttcgcgc cgaagtccag
     6061 cagcttctcg accgtgtact gcgccacgtt gcccgagccc gacaccaggc acaccttgcc
     6121 gtccagcgtg tcgccacggg tgtggagcat ctgctcggcg aagtacaccg cgccgtaccc
     6181 ggtggcctcg ggccggatca gcgaaccgcc ccagtccagc cccttgccgg tgagcgaacc
     6241 ggtgaactcg ttgcggatcc gcttgtactg accgaacagg tagccgatct cccgaccccc
     6301 cacgccgatg tcgcccgccg gcacgtcggt gtgcggaccg atgtggcgct gcagctcggt
     6361 catgaacgac tggcagaacc gcatcacctc ggcgtcggac ttgcccttcg gatcgaagtc
     6421 ggcgccgccc tttcccgcgc cgatcggcag cgtggtgagc gcgttcttga agatctgctc
     6481 gaacgccagg aacttcagca tgcccaggta caccgtgggg tggaaccgca gaccaccctt
     6541 gtacgggccg atcgcgctgt tcatctcgac gcggatgccg cggttgacgt gcacctcgcc
     6601 gtcgtcgtcc tgccacggca cccggaacat gatcacgcgt tcgggttcga tcagccgttc
     6661 caggatcgcg ccttcgcggt acttcggatg acgctcgagc accggagcga gggagtcggt
     6721 cacctcctgc accgcttgca ggaattcggg ctcgttcggg ttcttggccg tcacttcggc
     6781 catgagccga cggacgtact cactcaccag ctatcgcccc ccgggcgtgt cggtcgttcg
     6841 ggaaagtcta tgcgaggcag tcgttcagaa ccagggtcag gcggccggga cggtccgcgt
     6901 caggaacgcg aggatcgcgc gctgctggtg aatctcctcc tcgatgtcga acgagg
//
LOCUS       DATDBZ010000497         2455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1176016, whole genome shotgun sequence.
ACCESSION   DATDBZ010000497 DATDBZ010000000
VERSION     DATDBZ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176016"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1209
                     /locus_tag="VIX62_11675"
     CDS             127..1209
                     /locus_tag="VIX62_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY5860935.1"
                     /translation="MDLIPSPRTIRTRDLRLIAKIGLAAGMAWWVCTLLGAERPAFAA
                     IVPLVALRADDPFGALGVTVLRIVGTLAGILLGIIALLIDPNAPLWLVFATIAVGLAI
                     GLVARGSNEALNPITAVTALVVIFVGKGRADTYAWVRIWETLVGAAITMVVAVAVWPP
                     DPIAALRDLLRDLRRDVADDLRNVAELPGRSIDEADHMLDERIRRSMDTGDVTRTLDR
                     ARSGLRWNPRHRGRRVELVALAVPLRQLAAMSRYSRSLVWSMIGDPEGDHIRRWAPDA
                     EAALDEAMRHADDAAGDVADGRDPSASIAASDQALERFASLAGQRQLATDIHGGGRAM
                     LRVLSATTSRRLGALMRERYLPGEPS"
     gene            1212..2255
                     /locus_tag="VIX62_11680"
     CDS             1212..2255
                     /locus_tag="VIX62_11680"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5860936.1"
                     /translation="MELLHPRTAADVVEMVRESGARGTRLLPVGGRRHIDKGNPCEID
                     AELWTTQLDEVIAYDPAEMLAVVGAGMRMGGLRTLLAEGAQEWPVDAPDDATVGGVIA
                     AGLASPRQLRVGAMRDSVVQLTAVLGDGREVTSGARTVKNVSGYDIHRLMTGSLGTLG
                     VVVSAALKVRPLPRASMTLVARGDGLELATLVSTVAPTSPVAFTPGEVRVRLEGWPAE
                     VDELAGSVAAVSDAPFERSDGSQFPGWHTVAPIVAEVSMAPSAMDGTLRGHDDRCVLA
                     GVGSAKVGLADLDSLAALRERVTSSGGIAPVIHGPGGLGDAPVPALEVHRRLKAAFDP
                     HGVMAPGRFWSGL"
BASE COUNT          349 a          848 c          863 g          395 t
ORIGIN      
        1 cgaccccgag ggcctgatga acccgcagaa ggtgcttccc gacggagcac ggtgcggtga
       61 cttcgcgatc gcccgcgccg ggaccgacga cgctgcgcga gcggcacgaa ccctgccgga
      121 gggctcgtgg atctgatccc ctcgccccgc acgatccgca ccagggatct gcgcttgatc
      181 gccaagatcg gactcgcggc agggatggcg tggtgggtgt gcaccctcct cggcgccgag
      241 cgtcccgcgt tcgccgcgat cgttccgctg gtcgcgctgc gtgcggacga tcccttcgga
      301 gccctggggg tcacggtgtt gcgcatcgtg gggacccttg ccggcatcct gctcggcatc
      361 atcgccctgc tgatcgaccc gaacgcaccg ctgtggctgg tgttcgcgac gatcgcggtg
      421 ggcctcgcga tcggcctggt ggcccgcggg tcgaacgaag cgctcaaccc catcaccgcg
      481 gtcaccgcgc tcgtcgtgat cttcgtcggc aagggcaggg ccgacaccta cgcgtgggtc
      541 cggatctggg aaacgctcgt cggggccgcc atcacgatgg tggtggcggt cgcggtgtgg
      601 ccacccgatc cgatcgctgc gttgcgtgac ctgctgcgcg atctccgtcg cgacgttgcc
      661 gatgacctgc gcaacgtggc cgagctcccc ggccgctcga tcgatgaggc agaccacatg
      721 ctcgacgaac ggatccgcag gtccatggac accggggacg tcacgcgaac cctcgaccgg
      781 gcacgctcgg gactccggtg gaaccctcgc catcgagggc ggcgggtcga actcgtcgca
      841 ctcgcggtgc cgctgcgtca gttggccgcc atgtcgcggt actcccgctc gctcgtatgg
      901 tccatgatcg gcgaccccga aggcgatcac atccgtcgct gggcccccga cgctgaagcc
      961 gcgctcgacg aggccatgcg ccacgccgac gatgcggcag gtgacgtggc cgacggtcgc
     1021 gacccctccg cctcgatcgc ggcgtccgac caggccttgg aacggttcgc atcgctcgcg
     1081 ggccagcgtc agttggccac cgacatccac ggtggcggac gcgccatgct ccgcgtcctg
     1141 agtgccacca cgtcccggcg attgggtgcc ctgatgcggg agcgatacct gccgggcgaa
     1201 ccatcgtgac cgtggagttg ctccaccccc gcacggctgc cgacgtcgtc gagatggttc
     1261 gggagtccgg tgcgcgcggc actcgactcc ttcctgtcgg tggtcgccgg cacatcgaca
     1321 aagggaaccc gtgcgagatc gacgcggaac tctggaccac ccagttggac gaggtgatcg
     1381 cgtacgaccc tgccgagatg ctcgccgtcg tgggcgctgg catgcggatg ggtggtctgc
     1441 ggaccctgct ggccgaaggg gcacaggaat ggccggtcga tgcgccggac gatgccaccg
     1501 ttggaggagt gatcgcggcg gggctcgcat cgccgcgcca gcttcgcgtg ggcgcgatgc
     1561 gcgattcggt cgtccaactc accgccgtgc tcggcgacgg acgggaggtc accagcggcg
     1621 cccgaaccgt caagaacgtg agcggctacg acatccaccg gctgatgacc ggttcgctgg
     1681 gcacgctagg cgtcgtggtc agcgcggcgc tgaaggttcg cccgctcccc agagcatcga
     1741 tgaccctcgt cgctcgcggg gatgggctcg agctggcgac cctcgtcagc accgttgcgc
     1801 ccacgtcgcc ggtcgccttc acccctggcg aggtccgggt tcggctcgag gggtggccgg
     1861 ccgaggtcga cgaactggca ggctcggtcg ccgccgtgag cgacgcgccg ttcgagcgat
     1921 cggacggatc ccagttcccg gggtggcaca ccgtggcgcc catcgtggca gaggtgtcga
     1981 tggccccttc cgccatggac gggacgctca ggggccacga cgatcgatgc gtgttggcgg
     2041 gcgtggggtc agcgaaggtg ggcctcgccg acctggattc gctggcggcg ctcagagagc
     2101 gagtcacttc ctccggcggg atcgcccctg tgatccacgg cccaggaggc ctcggcgacg
     2161 cccccgtgcc cgccctcgag gtgcatcgac ggctcaaagc ggccttcgac cctcacggcg
     2221 tgatggctcc tggccggttc tggtccggcc tctgaaacca tcggatcggt gatggactgg
     2281 tccgatacag actagttcgc gtgggatgcg cggaggcgct tccctgcttc ctgcagcgtc
     2341 tcgatcgtct tgcagaaggc gaatcgcacc aggtgggctc cgtcggccgg gtcggcgaag
     2401 aacgaggagc caggcacgca cgtgacgccc acctgctcca ccatggtcgt cgcca
//
LOCUS       DATDBZ010000498         4761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2397853, whole genome shotgun sequence.
ACCESSION   DATDBZ010000498 DATDBZ010000000
VERSION     DATDBZ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2397853"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VIX62_11685"
     CDS             complement(<1..498)
                     /locus_tag="VIX62_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY5860937.1"
                     /translation="MKQVRAEILSTRKLGAYHSLTLVAPEIAEKARPGQFLAVKMPEG
                     REFLLRRHFAIHQASRRGGWAGTLEFVVDPTGSGTEWLTTAKAHEFLEVIGPLGKAFA
                     YPKKLTNCLLIAEGHGASSLYFLAQELHARQKRVDMVVGAETLERVFKPIEAKRLSQS
                     VAIMTE"
     gene            complement(495..3800)
                     /gene="carB"
                     /locus_tag="VIX62_11690"
     CDS             complement(495..3800)
                     /gene="carB"
                     /locus_tag="VIX62_11690"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY5860938.1"
                     /translation="MPRREDIRRIMVVGSGPIVIGQACEFDYSGTQACKVLRAEGYEV
                     ALVNSNPATIMTDPQFADRTYIEPLTPASVLAILERERPDALLPTLGGQTALNVAVAL
                     AESGDLDRLGVQLIGASLEAIHRAEDRSGFKEAMVKAGLDVPRAGIAHTLEEAQGLGE
                     ELGFPLIVRASFTMGGGGSGFAANHEELAALAVRALEISPVSEILIEESIAGWKEFEL
                     EVMRDGDDNGVIVCSIENVDPMGVHTGDSITVAPQQTLTDREYQQMRDTALQVLRVIG
                     VETGGSNVQFAVDPVTGRQVLIEMNPRVSRSSALASKATGFPIAKIAALLAVGYRLDE
                     ITNDITGETPVSFEPTLDYVVVKIPRFAFEKFPEADPHLTSTMKSVGEVMAIGRTFKE
                     ALGKAWRGIEKTGFDLGSTLAEETEPMEALAAGTEHRLQVVQAALEAGRTVDEVATAS
                     AIDPWFVDQIAEIVEEAATLRGRALSTLSARDLWSAKRLGLSDAHLAMLTSSTEAAVR
                     RHRDALGVSPVFKTVDTCGGEFPARTPYHYSTYEEESEVLPAARPRVVILGAGPNRIG
                     QGIEFDYACVHAAFALEEAGFESVMVNSNPETVSTDYDTSSRLYFEPLSPEDVLAVCR
                     AEQPVGVIAQLGGQTPLRLARILQDEGYAIWGTSPGAIDLAEDRGKFARVLAELEIPA
                     PPHGEARTMAEARAIAEQIGYPVVVRPSYVLGGRAMEIVYDADDLEAFVQTASDASPD
                     HPVLIDRFLEGAIEVDVDAVADATGDVFIGGVMEHIEEAGVHSGDSSCQIPPATLSDE
                     ELDVIEDITRRLARRLGVVGLLNLQLAVKDERIWVIEANPRASRTVPFVSKVIGVSLA
                     RIATLVLAGRTLLDLGADGVLPADPHHYRHLPYASVKAAVLPFGRFPGVDTVLGPEMK
                     STGEVMGIDADSGWALAKAFTAAGAGLPSEGTVFVSVANRDKRAIVFPAKRLADLGFR
                     LLATGGTAGVLHRAGIPVERVAKVSEHADNVAELIRGGSVDLVINTPFGRGARTDGYF
                     IRTASASAGVPCVTTLPGVFAAVRGIEALRGRSTEPRSIQEYHAEAMQEPMQARMTFS
                     EAAAAGVAGESPA"
     gene            complement(3800..>4761)
                     /gene="carA"
                     /locus_tag="VIX62_11695"
     CDS             complement(3800..>4761)
                     /gene="carA"
                     /locus_tag="VIX62_11695"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY5860939.1"
                     /translation="MNEADPESVRVQVAGFAVREVARRASSWRSEGTLGDALAGSGVT
                     GIEGIDTRRLTRRLRDGGAMRAVISTVDLDPSSLAERVQAAPGMAGADLARTVSATKP
                     YEAVALTGPANTDRGRVFRVAAYDFGIKRNILRLLAASGIETTVFPAQTPAAAVAAGG
                     YDGVFLSNGPGDPSVTAYGIGATRALLGKVPIFGICLGHQLLAHALGGRTFKMKFGHR
                     GVNQPVKNLQTGVVEITSHNHGFAVDPDGWVRDADGVATTDLGRAALTHWNLNDGTLE
                     GLRCLDLPAFSVQYHPEAAPGPHDSRYLFEDFRALMGAAGEGT"
BASE COUNT          713 a         1698 c         1628 g          722 t
ORIGIN      
        1 ctcggtcatg atcgcgaccg actgcgacag gcgcttggcc tcgatcggct tgaacacccg
       61 ctccagggtc tccgcgccca ccaccatgtc gacgcgcttc tgccgcgcgt gcagttcctg
      121 ggccaggaag tacagggacg aggcaccgtg gccctcggcg atcaacagac agttggtgag
      181 cttcttcgga tacgcgaacg ccttgccgag cgggccgatg acctcgagga actcgtgcgc
      241 cttcgcggtg gtgagccact cggtgcccga gcccgtgggg tcgacgacga actccagcgt
      301 cccggcccag ccgccgcggc gcgaggcctg gtggatcgcg aaatgtcgcc gcaggaggaa
      361 ctcacggccc tcgggcatct tgaccgccag gaactgccct gggcgggcct tctcggcgat
      421 ctcgggcgcg accagggtca gggagtggta ggcacccagc ttgcgcgtgg acaggatctc
      481 ggcgcgaacc tgcttcacgc gggagactcc cccgcgaccc cggccgcggc ggcctccgag
      541 aacgtcatcc gggcctgcat cggctcctgc atcgcttcgg catggtactc ctggatcgaa
      601 cgcggctccg ttgaccgccc gcgtagcgcc tcgatgccac gcaccgccgc gaacacgccc
      661 ggcaacgtgg tgacgcacgg cacgccggcc gaggccgagg ccgtccggat gaaatagccg
      721 tccgtgcgcg ccccccggcc gaacggcgtg ttgatcacca gatcgaccga gccaccccgg
      781 atcagctcgg cgacgttgtc ggcgtgctca ctcaccttgg ccacccgctc gaccgggatc
      841 ccggcgcgat gcagcacgcc ggcggtgccg ccggtggcca gcaaccggaa ccccaggtcg
      901 gcgaggcgct tggccgggaa cacgatcgcg cgcttgtccc ggttggccac gctcacgaac
      961 acggtgccct cgctcggcag ccctgccccg gcggcggtga acgccttcgc caacgcccag
     1021 ccggaatcgg cgtcgatgcc catgacctca ccggtcgact tcatctccgg cccgaggacg
     1081 gtgtcgacgc ccgggaaccg tccgaacggc aggacggccg ctttcacgct ggcgtacggc
     1141 aggtgccggt agtgatgcgg gtcggccggc agcaccccgt cggcacccag gtcgaggagc
     1201 gtccggcccg cgagcaccag cgtggcgatc ctggcgagcg agacgccgat caccttcgag
     1261 acgaacggga cggtccgcga tgctcgcggg ttcgcctcga tcacccagat gcgctcgtcc
     1321 ttgaccgcga gttgaaggtt gagcagcccc acgaccccga ggcggcgagc cagccggcga
     1381 gtgatgtcct cgatcacgtc gagctcctcg tcggacagcg tcgcgggcgg gatctggcac
     1441 gaggagtcgc cggaatgcac accggcttcc tcgatgtgct ccatgacccc gccgatgaac
     1501 acgtcgccgg tggcatcggc caccgcgtcg acgtcgacct cgatcgcgcc ttccaggaag
     1561 cggtcgatca ggaccgggtg atcgggcgag gcgtcggaag ccgtctggac gaacgcctcg
     1621 aggtcgtcgg cgtcgtacac gatctccatc gctcgcccgc ccagcacgta cgacggccgg
     1681 accaccacgg gatagccgat ctgctcggcg atggcgcggg cctcggccat ggtgcgggct
     1741 tccccgtggg gtggcgcggg gatctcgagc tcggcgagca cccgcgcgaa cttgccgcga
     1801 tcctcggcca ggtcgatcgc gccgggcgac gtgccccaga tggcgtagcc ctcgtcctgc
     1861 aggatgcgag ccaagcgcaa cggggtctgt cccccgagct gggcgatcac ccccaccggc
     1921 tgctctgcgc gacacaccgc cagcacgtcc tcgggcgaca acggctcgaa gtacagacgg
     1981 ctcgaggtgt cgtagtcggt ggagacggtc tccgggttgg agttcaccat caccgattcg
     2041 aagccggcct cttccagggc gaaggcggca tggacgcacg cgtagtcgaa ctcgatcccc
     2101 tggccgatcc ggttcggacc ggcgcccagg atcaccacgc gggggcgcgc cgcgggcagg
     2161 acctcggatt cttcctcgta ggtggagtag tggtagggcg tgcgcgccgg gaactcgcca
     2221 ccgcacgtgt cgacggtctt gaacaccggc gagaccccca gcgcgtcgcg atgccggcgg
     2281 acggccgcct cggtcgagga cgtgagcatc gcgagatggg catcggacag gccgagccgc
     2341 ttcgcgctcc agagatcgcg ggcgctcaac gtcgacagcg ctcggccacg cagggtggcg
     2401 gcctcttcca cgatctcggc gatctggtcc acgaaccacg ggtcgatggc gctggccgtg
     2461 gcgacctcgt ccacggtgcg gcccgcttcg agggcggcct gcaccacctg cagccggtgc
     2521 tcggtgccgg cggcgagcgc ctccatcggc tcggtctcct cggcgagcgt cgagcccagg
     2581 tcgaacccgg tcttctcgat gccgcgccac gccttgccca gcgcctcttt gaaggtccgg
     2641 ccgatcgcca tcacctcgcc caccgacttc atcgtcgacg tgaggtgcgg gtcggcctcg
     2701 gggaacttct cgaacgcgaa ccgcgggatc ttcacgacga catagtcgag cgtgggctcg
     2761 aacgagacag gcgtctcgcc ggtgatgtcg ttcgtgatct cgtccagccg gtagcccacg
     2821 gccaggagcg ccgcgatctt cgcgatcggg aacccggtgg ccttcgacgc cagcgcgctg
     2881 gagcgtgaga cccgagggtt catctcgatc aacacctgac ggccggtgac cgggtcgacc
     2941 gcgaactgga cgttggagcc gccggtctcg acgccgatca cgcggagcac ctggagcgcg
     3001 gtgtcgcgca tctgttggta ttcacgatcg gtgagcgtct gctggggcgc caccgtgatc
     3061 gagtcgccgg tgtgcacgcc catcggatcg acgttctcga tcgagcagac gatgacgccg
     3121 ttgtcgtcgc cgtcccgcat cacctcgagc tcgaactcct tccagcccgc gatggattcc
     3181 tcgatcagga tctccgagac cggggagatc tcgagcgcac gcacggcgag cgcggcgagc
     3241 tcctcatggt tcgcggcgaa cccgcttccc cctccgccca tcgtgaacga ggcgcgcacg
     3301 atcaacggga acccgagttc ctcgcccagc ccctgggcct cctcgagggt gtgggcgatg
     3361 cccgcccgcg gcacgtcgag gccggctttc accatggctt ccttgaaccc gctgcggtcc
     3421 tccgctcggt ggatcgcttc cagggacgcg ccgatcagct gcacgcccag ccggtcgagg
     3481 tcgcccgatt cggcgagcgc caccgccacg ttcagcgcgg tctggccacc gagggtgggc
     3541 agcagcgcgt cggggcgctc gcgctccagg atcgccagca ccgacgccgg cgtgagtggt
     3601 tcgatgtacg tccggtcggc gaactggggg tcggtcatga tcgtggcggg gttggagttc
     3661 accagcgcca cctcgtaccc ctcggcccgc agcaccttgc acgcctgcgt tcccgaatag
     3721 tcgaactcgc acgcctggcc gatcacgatc ggccccgagc cgaccaccat gatcctgcgg
     3781 atgtcttcgc gcctcggcat caggttccct cccccgccgc ccccatcagg gcgcggaagt
     3841 cctcgaacag gtagcgcgaa tcgtgcgggc ccggcgctgc ctcggggtgg tattggacgc
     3901 tgaaggccgg caggtcgagg caccgcaggc cttccagcgt gccgtcgttg aggttccagt
     3961 gggtgagggc ggcgcgcccc agatcggtcg tggcgacgcc gtcggcatcg cgaacccacc
     4021 cgtcgggatc gacggcgaat ccgtggttgt ggctggtgat ctccacgacg cccgtctgca
     4081 ggttcttcac cggttggttc acgccccggt ggccgaactt catcttgaag gtgcggccgc
     4141 cgagggcatg ggcgagcagt tggtggccca ggcagatccc gaagatcggc accttgccga
     4201 gcaacgcacg cgtggccccg atgccgtagg cggtcaccga cggatcaccg gggccgttgg
     4261 acaggaacac accgtcgtag ccgccggccg ccaccgccgc ggcgggcgtc tgagcgggga
     4321 acacggtggt ctcgatcccg ctcgctgcga gcagccggag gatattccgc ttgatcccga
     4381 agtcgtaggc cgccacgcgg aagacacggc cccggtcggt gttggccggt cccgtgagcg
     4441 ctaccgcctc gtagggcttg gtggccgaca ccgttcgggc gaggtcggca ccggccatgc
     4501 cgggcgccgc ctgcacgcgc tccgcgagcg aggaagggtc gaggtcgacg gtggagatca
     4561 ccgcgcgcat cgcgccacca tcgcgcaggc gtcgcgtcag ccgccgtgtg tcgatgcctt
     4621 cgatgccggt gacccccgaa ccggcgagcg cgtcacccag ggtgccttcc gatcgccagg
     4681 acgaggcgcg gcgcgccacc tcgcgaacgg cgaaccccgc gacctgcacc cgcaccgatt
     4741 cgggatcggc ctcgttcatg c
//
LOCUS       DATDBZ010000499         5274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1386461, whole genome shotgun sequence.
ACCESSION   DATDBZ010000499 DATDBZ010000000
VERSION     DATDBZ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386461"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VIX62_11700"
     CDS             complement(<1..419)
                     /locus_tag="VIX62_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEY5860940.1"
                     /translation="MPDLGDLVRAAVEAAGDGEAVEAYAQESRQTEASALRGEVEGLT
                     FAESRGLGVRLIRDGRLGYAFAADPTVDEVRAAVVKARENATLAEPDEFNVLPDAAEV
                     TALPELFDAASADVPTADKVQMALDLESRLVSSDPRVT"
     gene            complement(412..1797)
                     /locus_tag="VIX62_11705"
     CDS             complement(412..1797)
                     /locus_tag="VIX62_11705"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY5860941.1"
                     /translation="MLDRSLVHEVLQAARRRGGSFAEVFVEETTNTTIRLDDGKVEEL
                     TTGLDRGAGVRVAHGTSYGYAYSNRLDRDALLEAAEAASAALRDGEGGDVVDLRSLEG
                     PDTNRVQRAAASVPAADKVAWLRELDDVARSFSPEVIQVVGVYGDSLQRRLIAASDGR
                     WVEEARPRIRLVAQVVAKRGDNIQTGFHGPAACAGVEFVDEHTPAATATVAAKRAVTM
                     LDSIPAPAGEMTVVLAPGMGGVLFHEAVGHPLESDAIDKEASVYAGLVGQRCGSELLN
                     GVDDATIPNGWGSFTFDDEGEPAQNTVLFTDGVLQGFLYDRLRADKEGVTSTGNGRRQ
                     SYAFPPVPRMTNTNILNGSSKPGDILDATEQGVWVTSLGGGQVNPATGDFVFGVSEGF
                     LIENGQVGAPVRGVNLIGRAIEVMSAIDAIADDFGTWEGVCGKDGQNAPVGSGSPTLR
                     ISKITLGGTGA"
     gene            1985..2587
                     /locus_tag="VIX62_11710"
     CDS             1985..2587
                     /locus_tag="VIX62_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860942.1"
                     /translation="MRTRSTVVTILFFSVLGAACRGDEQPPTFSFSPISPPVTGPAVT
                     SPAVTGPAGTGATGATGAPPTGSPGVTGAVNTGTASVSTTGVLTTQVAYTTLASPAIW
                     AVPPGGMALNWTATRNQALGISGASFTAQQPTATERLLQFSVQGPDGLVLFQSSAGEC
                     LVTITPALANQMGGTFLCTNIQSEDGSITVNAQGSFSAAG"
     gene            2724..4427
                     /locus_tag="VIX62_11715"
     CDS             2724..4427
                     /locus_tag="VIX62_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860943.1"
                     /translation="MYLWWARVGAAEGISLVGARPGTPALIPTVAGALHMGLVPALAG
                     LQYALGAAIGTATIALVRGRSHGGRPAWLLAGLFAGMFGVHLAGGYVANLAYTLPFIA
                     AAAALARRSRRGTIGAGLLLGGGGLSHPQFFLVGAFILIVSAAMAWILEPEHGWRSDA
                     GRVLAALGGGGVVVAAGLLSMVIGPPQLSVDTSKDGFLRRAGLADALHETYQFRFREN
                     VRRYAPWVTLPLAAVGTLQVRGFTRRFLVAWLACTIIGVPLGIATGWFPPERLMTFGF
                     ALPMLAALGVTWVWERTEPRRWLTVVATGILVALFAVPTIDAQRDQQTFMSPEDLSSG
                     AEAGRIAATLPPGTPLVFVVDDLDTSATFLATHVANIARATVPPDRVGDVHVFVGRVP
                     DYFLGRPTVKGAEEYDALSAITLADLPPGPRALFVVHEFDRDPAAFADPHLYAWTDGV
                     WSDVPAPRPLPSLPGELRASAPWPIAGATVAVLALLWLIGAGWASWTFGDPVAAAAAA
                     PAFGVAILTIVALALERIGVPLTGSWGPTIACALAGLGGYGLRFLQGKARVDPSSQID
                     Q"
     gene            complement(4320..>5274)
                     /locus_tag="VIX62_11720"
     CDS             complement(4320..>5274)
                     /locus_tag="VIX62_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860944.1"
                     /translation="ASLATLAMILVTAERVRPGRVTFAAAAYGLNPVVLFLAVGSGHN
                     DVLVALSIIGAVALLTSRREMWAVAVLALGALVKATAVLPLVLVIVWCVARRPKGERM
                     RAFASHAGLAVGIGLVFGLPYLQTHDPTLGMLELAGHTGWLAPSVFVEKVVDFFSFGT
                     LGWLARVGFAITLVVCVVELARTVAARASAGDAIEELTAAIGWSLVFLMLLGPVLLPW
                     YVVWVLPVVWLLPRAPRATVITAGAALAMAQWSTEPLRYPDAFNLNLWLGHWVVTPVM
                     FVMVIWSLVDLRRRIHARLPLEESEPIPAEARQGAGDRGTP"
BASE COUNT          751 a         1920 c         1808 g          795 t
ORIGIN      
        1 gtgacccgcg gatcgctcga gaccaaccgg gactcaaggt cgagcgccat ctgcaccttg
       61 tcggcagtgg gcacgtcggc cgatgcggcg tcgaacagct ccggcagcgc cgtcacctcg
      121 gcggcgtccg gcagcacgtt gaactcgtcc ggctcggcga gcgtggcgtt ctcgcgcgcc
      181 ttgaccacgg ccgcccggac ctcgtcgacc gtgggatcgg cggcgaaggc gtagccgagc
      241 cggccgtcac ggatcaaccg cacgccgagc ccgcgggact cggcgaacgt cagcccttcg
      301 acctcaccgc ggagggcgct cgcttcggtc tggcgtgact cctgggcgta ggcctccacg
      361 gcctcaccat caccggccgc ttcgaccgcc gcgcgcacga gatccccgag atcaggcacc
      421 ggtgcctccc agcgtgatct tggagatccg caaggtgggg gaaccgctgc ccaccggcgc
      481 gttctgtccg tccttgccgc acacgccctc ccaggtgccg aagtcgtcgg cgatcgcatc
      541 gatcgcgctc atgacctcga tcgcccggcc gatcaggttc acgccgcgaa ccggggcgcc
      601 aacctggccg ttctcgatca agaagccttc actgaccccg aacacgaagt cgcccgtggc
      661 ggggttgacc tgtccgccgc cgagcgaggt cacccacacg ccctgctcgg tggcgtcgag
      721 gatgtcgccg ggcttggagg aaccattgag gatgttcgtg ttggtcatgc gcggcacggg
      781 agggaaggcg taggactggc gtcggccgtt cccggtggac gtcacgccct ccttgtcggc
      841 tcgcagccgg tcgtagagga acccctgcag cacgccgtcg gtgaacagca ccgtgttctg
      901 cgccggctcg ccttcgtcgt cgaacgtgaa cgaaccccag ccgttgggga tcgtggcgtc
      961 gtccacaccg ttgaggagtt cgctgccgca ccgttggccc accaagcccg cgtacacgct
     1021 ggcctctttg tcgatggcgt cggactcgag cggatgcccg accgcctcgt ggaacaggac
     1081 gccgcccatg ccgggcgcga gcaccacggt catctcgccg gccggcgcgg ggatcgagtc
     1141 gagcatcgtc acggcgcgct tggccgccac ggtggcggtg gccgccggcg tgtgttcgtc
     1201 cacgaactcg acgccggcac acgccgccgg tccgtggaac ccggtctgga tgttgtcgcc
     1261 gcgcttggcc accacctgtg cgaccaggcg gatccggggc cgcgcctctt cgacccagcg
     1321 gccatcggag gcggcgatca accgccgctg caaggaatcc ccgtagacgc ccaccacctg
     1381 gatcacctca gggctgaacg aacgggcgac atcgtcgagt tcccgcagcc acgcgacctt
     1441 gtccgcggcc ggaaccgacg cggccgctcg ctgcacccgg ttcgtgtcgg ggccctcgag
     1501 ggatcgcaga tcgacgacat cgcctccctc accgtcgcgc agcgcggcgc tggcggcttc
     1561 ggcggcctcg agcaacgcgt cgcggtccag ccggttggag tacgcgtagc cgtatgacgt
     1621 gccgtgggcg acccggacgc cggcgccgcg atcgaggccg gtggtgagtt cctcgacctt
     1681 gccgtcgtcc agccggatcg tggtgttcgt ggtctcctcc acgaagacct cggcgaacga
     1741 cccaccgcgc cgacgagcgg cctgcagcac ctcgtgcacg agcgagcgat cgagcatgac
     1801 ccgcgatgct actagcagtc gtcaggcgat gcccccgggg cgatccggtg cgtcccttcc
     1861 cgcgaatcgg tgcagtacag tcagccacgc gaacgcggcc gggacgcagc agcgccgagc
     1921 gccgaccggt cgagaacgag gccagaacac tgaggccaga acactgagga aggtcacgcg
     1981 cgcgatgcga accagatcca cggtggtcac gatcctgttc ttctccgtgc tgggtgcagc
     2041 atgccgaggc gatgagcaac cccctacctt ttcgttctcg cccatctcgc cgccggtcac
     2101 cggccccgcg gtcactagcc cagcggtcac cggccccgcg gggaccggtg cgacgggggc
     2161 gacgggagcg ccacccacgg gctcgcccgg ggtgaccggc gcagtcaaca ccggcaccgc
     2221 ttcggtctcc accaccgggg tgttgacgac acaggtggcc tacacgacgc tcgcgtcgcc
     2281 ggcgatctgg gccgtgcccc cgggtgggat ggcgctcaac tggaccgcca cccgcaacca
     2341 ggcgctcggc atctccggtg cctcgttcac cgctcagcaa cccaccgcca ctgaacgact
     2401 cctgcagttc tcggtccagg ggccggacgg gctcgtcctc ttccagtcga gcgcgggtga
     2461 atgtttggtc acgatcaccc cggccctcgc caaccagatg ggcggcacgt tcctgtgcac
     2521 gaacatccag agcgaggacg ggtcgatcac ggtgaacgcc cagggctcct tcagcgcggc
     2581 ggggtaagcg cacggacgcc ccggccggcc acgagcgtcg agatctcctg tggctcctcg
     2641 cgctggtcgc ggtcctgctc gggttcttcc tgtggcccac gttcgcctat cgctggccgt
     2701 tcggcgtcgg gcccgacgaa cccgtgtacc tctggtgggc ccgcgtgggc gcggccgaag
     2761 gcatctcctt ggtgggagcc cggcccggta ccccggcgtt gatcccgacg gtggccggcg
     2821 ccctgcacat ggggctcgtc ccggcgttgg caggactgca gtacgcgttg ggggcagcga
     2881 tcgggaccgc cacgatcgcg ctggtgcgtg ggcgctcgca cggaggacgg cccgcgtggc
     2941 tgctagcggg gttgttcgcc ggcatgttcg gggtgcatct ggcgggcggc tacgtcgcga
     3001 acctcgccta cacgctcccc ttcatcgcgg ccgccgcggc gttggcgcgt cgcagccgtc
     3061 gaggcacgat cggcgcaggc ctgctgctgg gcggcggagg actctcccat ccccagttct
     3121 tcctcgtggg cgcgttcatc ctgatcgtgt cggccgccat ggcctggatc ctcgaaccgg
     3181 agcacgggtg gcgctcggac gcgggccgcg tgctggctgc gctcggcggc ggcggcgtgg
     3241 tcgtggccgc gggtctgctg tccatggtga tcggacctcc gcagctgtcc gtcgacacgt
     3301 cgaaggacgg gttcctgcgc cgcgccggtc tcgctgacgc gttgcacgag acctaccagt
     3361 tccggttccg ggagaacgtg cgccggtacg ccccgtgggt gaccttgccg ctggccgccg
     3421 tcggcacgct gcaggtgcgc gggttcacgc gccggttcct ggtggcatgg ctcgcctgca
     3481 cgatcatcgg cgtgccgttg ggcatcgcga cgggctggtt cccacccgag cggctgatga
     3541 cgttcggctt cgcgctgccg atgctggcgg cgctgggggt gacctgggtc tgggaacgca
     3601 ccgaaccgcg ccgctggctc accgtcgtgg cgacggggat cctggtggcg ctgttcgccg
     3661 tgcccacgat cgacgcccag cgcgatcagc agaccttcat gtcccccgag gacctgagct
     3721 cgggcgccga agcgggacgg atcgccgcga ccctgccgcc cggcacgccg ttggtgttcg
     3781 tggtcgacga cctggatacg tccgcgacgt tcctggctac ccacgtggcg aacatcgcac
     3841 gagccaccgt cccgccggac cgcgtggggg acgtgcacgt gttcgtgggg cgggtgcccg
     3901 actacttcct ggggcggccc accgtgaagg gcgccgagga atacgacgcc ctttcggcga
     3961 tcacgctggc cgatctgccc ccaggtccgc gtgccctctt cgtggtgcac gagttcgatc
     4021 gcgatccggc cgccttcgcc gacccccacc tgtacgcctg gaccgatggg gtgtggagcg
     4081 acgtcccggc accccggccg ttgccgtcgc tacccggcga gctccgcgca tcggcgccgt
     4141 ggccgatcgc gggcgcgacc gtggcggtcc tggcgttgct ctggctgatc ggagcgggtt
     4201 gggcgtcgtg gacgttcggt gatccggtcg cggccgcagc cgcggcgccg gcgttcggcg
     4261 tcgccatcct cacgatcgtg gcactcgcct tggagcggat cggggtcccc ctcacggggt
     4321 catggggtcc cacgatcgcc tgcgccctgg cgggcctcgg cgggtatggg ctccgattcc
     4381 tccaggggaa ggcgcgcgtg gatccgtcgt cgcagatcga ccagtgacca gatcaccatc
     4441 acgaacatca ccggcgtgac cacccagtgc ccgagccaca ggttcaggtt gaacgcatcg
     4501 ggataccgca ggggttcggt cgaccattgc gccatcgcga gggcggcgcc ggccgtgatc
     4561 accgtggcgc ggggcgcgcg aggcagcagc cacacgaccg gcaggaccca caccacgtac
     4621 cacggcagca gcaccggccc cagcagcatc aggaacacca gcgaccaccc gatcgctgcg
     4681 gtgagctcct cgatcgcatc gcccgcggac gctcgagccg ccaccgtcct ggccagttcg
     4741 acgacgcaca ccaccagggt gatggcgaac ccgacgcgcg cgagccaacc cagcgtgccg
     4801 aagctgaaga agtcgacgac cttctcgacg aacaccgacg gcgcgagcca tccggtgtgg
     4861 cccgcgagtt cgagcatgcc gagcgtcgga tcgtgggtct ggaggtaggg cagcccgaac
     4921 accaggccga tccccaccgc cagcccggca tgtgaggcga acgcccgcat gcgctcaccc
     4981 ttcggccgtc gcgccacgca ccagacgatc accagcacca acggcagcac cgccgtggcc
     5041 ttcacgagtg cccccagggc gagcacggcc accgcccaca tctcccggcg cgacgtgagc
     5101 agcgccaccg cgccgatgat cgacagggcg acgagcacgt cgttgtgacc gctgcccacc
     5161 gccaggaaca gcaccaccgg gttgagtccg tacgcggccg cggcgaacgt gacgcgcccg
     5221 ggccgcaccc gctcggcggt caccaggatc atcgccagcg tcgccaggct cgcc
//
LOCUS       DATDBZ010000500         5185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1809202, whole genome shotgun sequence.
ACCESSION   DATDBZ010000500 DATDBZ010000000
VERSION     DATDBZ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809202"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VIX62_11725"
     CDS             complement(<1..1284)
                     /locus_tag="VIX62_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5860945.1"
                     /translation="MGLFGFEGDNAREKVIVLLTMCFALAMAMLDNTVVNVALPTLNR
                     ELGASVSDLQWIVDGYVLALGSLLLTGGILGDRYGRKRMFLTGLTNFTLASLGSGMSQ
                     SSSQLLGFRVLQGVGAALLLPGTLSILTVTFPPHERAKAIGLWAGMSGLALALGPTLG
                     GYMVEHLGWESVFFLNVPIGIVAFIVATRTVRESRSDEARQLDIPGLVLGTGALFSMT
                     YALIEANQKGWSDPVIVAGLVGGVVLAIAFLVWESHSPHAMMPLRFFRIPAFSTGNLV
                     AFSISLGMFSTFFFLSLYMQSPFLRGYTAFEAGLRFLPMTLMIVVTAPNAGRYASKHG
                     SRAPMTYGLLLAGGGLVALSMLTTTTPYWIMAVIFAIMGHGMGATMAPMTAAVMNSVG
                     HERAGLGSAMTNTSREVGGVFGIALLGTILFTRLKS"
     gene            complement(1381..2247)
                     /locus_tag="VIX62_11730"
     CDS             complement(1381..2247)
                     /locus_tag="VIX62_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860946.1"
                     /translation="MSATVQQAPTQRRAPRNTAGRIALAFDDGLLVARRNLVTLTRVP
                     TVFVFELVQPIMFVLLFRFIYANQFSQLPPELSYVQFLMPGIFIQNALFQSTNTAIGL
                     AEDLKTGIIDRFRSLPMARSAVLVGRTGSDLIKNFLLLVIVIGIGYLVGFKFENGLFN
                     ALGVVLLVIAVGFAFSWISACIGLALKQVEAVQAASFTWIFPVVFVSSAFVPVAGMSG
                     FLQVVARNNPVTIWCNLARYLSSGSVAIVDFESKLPVDTFEGLVFKSTVWIIGLLVVF
                     VPLAIHLYRKLD"
     gene            complement(2244..3260)
                     /locus_tag="VIX62_11735"
     CDS             complement(2244..3260)
                     /locus_tag="VIX62_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5860947.1"
                     /translation="MPAIIRAEQVTKSFGEVHALKGVDLEVEKGTVLGLLGPNGAGKT
                     TMVRILATLLLPTGGRAEVDGFDVVKDAEELRFRIGLAGQSAAVDENLTGLENLQLVG
                     RLYHLEKGLAKQRAVEVLERFDLADAADRATKTYSGGMRRRLDLAASLVGRPDVLFLD
                     EPTTGLDPRSRIDVWDFIRELQSEGTTLLLTTQYLEEADVLADQIAVIDRGALIAQGT
                     SDELKEQVGGEVLELHVENRDDTSRVVEALKGIGTGDPSVDADEGAVRIPVGGEGPVA
                     LLESVRRLDETDIRLADIALHRPTLDDVFLKLTGRSAVEDEAEADGKDGKKEKRGRRG
                     GKEA"
     gene            3384..4400
                     /locus_tag="VIX62_11740"
     CDS             3384..4400
                     /locus_tag="VIX62_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860948.1"
                     /translation="MQRILTVIAITFLLVVSACGGGEDGASPTTTGAASAPPTDTASA
                     TPQPTIEATVPVGGEPIGISAGEGAIWVVNSEFESGGEPSVSRIDPATSKVTDTIAVG
                     ALPLEVLAAGGSVWVSNSEDGTVSRIDPSSAKVTATIEVCKAPEGLALAAGSVWVVCE
                     ESDAVGRIDPATDTMVDTVKVEDEPRFAAAAFGSVWVSNYLGSSVSRVDPASGKVTAT
                     IDVDLGGQVMAALDGSLWISSIDYNTLTRIDPETEKVEKVFNDVGDYPDGILASQGAL
                     WIASDLGPELRRFDPATGKVSGTWVIGEQGTINANQLLAEADGSFWLPLLESSEVARV
                     AIPD"
     gene            complement(4406..>5185)
                     /locus_tag="VIX62_11745"
     CDS             complement(4406..>5185)
                     /locus_tag="VIX62_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEY5860949.1"
                     /translation="ASSWIRHSGRLAIGLGAVALVAVLTAGSIDRLASWVAPVGRAVV
                     GALAWAVAQLARPIFWALERADLDTQGAADLLERIRRSSQEASRAAADRADPHGGGGG
                     FARVVGFLFLVGITAVAIMLFRRFRAKPSRAISPLDAPADASATVLPADDRPTGEPPS
                     RAARRTPSADAVRRAYADALDALEARGEPKPPDATPAEFAREVSARHPVVAEDFGALT
                     RAYEDVRYGAATLANDAVEAVERHRRSLVAAVRRLPPPAEA"
BASE COUNT          846 a         1742 c         1816 g          781 t
ORIGIN      
        1 gctcttgagc cgggtgaaca ggatcgtgcc gagcagcgcg atgccgaaca ccccgcccac
       61 ctcgcgactc gtgttggtca tggccgaccc caagccggcc cgttcgtggc cgaccgagtt
      121 catgaccgcg gcggtcatgg gcgccatggt ggctcccatg ccgtggccca tgatcgcgaa
      181 gatcacggcc atgatccagt acggcgtggt ggtggtcagc atcgagagcg ccaccagccc
      241 gccgccggcc agcaacaggc cataggtcat cggggctcga gagccgtgct tggaggcgta
      301 ccggccggcg ttgggcgcgg tgacgacgat catcaacgtc atcggcagga accgcaggcc
      361 ggcttcgaac gccgtgtaac cacggaggaa cgggctctgc atatagaggc tgaggaagaa
      421 gaacgtggag aacatgccca gcgagatcga gaacgccacg aggttgccgg tggagaacgc
      481 cgggatccgg aagaatcgca gcggcatcat ggcgtgcggg ctgtgggatt cccacaccag
      541 gaacgcgatc gcgaggacga cgccgcccac gagtccggcg acgatcaccg ggtcgctcca
      601 gcccttctgg ttggcttcga tcagcgcgta ggtcatggag aagagcgcgc cggttcccag
      661 gaccaggccc gggatgtcga gctgccgcgc ttcgtcggat cgcgattcgc ggacggtgcg
      721 ggtggccacg atgaaggcca cgatgccgat cggcacgttg aggaagaaca ccgactccca
      781 gcccaggtgt tccaccatgt agccgcccag ggtcggtccc agcgccagcg ccaggcccga
      841 catgccggcc cacaacccga tcgccttcgc gcgctcgtgc ggtgggaagg tgacggtgag
      901 gatggacaac gtgcccggca acaacaacgc ggcgccgacg ccctgcagga cccggaaccc
      961 gaggagctgg ctgctggact gcgacatccc gcttccgagc gaggccagcg tgaagttcgt
     1021 gaggcccgtg aggaacatgc gcttgcgtcc gtaccggtcg cccaggatcc cgccggtgag
     1081 cagcagggac ccgagcgcga gcacatagcc gtcgacgatc cactgcaggt cgctgacgct
     1141 ggcgcccagt tcccggttga gggtgggcaa cgccacgttc accacggtgt tgtcgagcat
     1201 ggccatggcc agcgcgaagc acatggtgag caggacgatg actttctctc tggcgttgtc
     1261 gccttcgaat ccgaacagac ccaacgttcc tccttgcggg atggtgcagg tcgcgggcag
     1321 cgacggaggc gttctggttg gcaaccgcct cggcgccggg aagattcccg acgaccggtc
     1381 tcagtcgagc ttgcggtaga ggtggatcgc gagcggcacg aagaccacca ggagcccgat
     1441 gatccagacc gtcgacttga acaccaggcc ttcgaacgtg tcgacgggca acttgctctc
     1501 gaagtccacg atggcgaccg agcccgagga caggtacctc gccaggttgc accagatggt
     1561 gaccgggttg ttgcgggcca ccacctgcag gaacccggac atgccggcga ccggcacgaa
     1621 cgccgagctc acgaacacca cggggaagat ccaggtgaac gacgccgctt ggaccgcctc
     1681 gacctgtttc aacgccaggc cgatgcaggc cgagatccac gagaacgcga agcccaccgc
     1741 gatcacgagc aggacgacgc cgagcgcgtt gaacagtccg ttctcgaact tgaagcccac
     1801 caggtaaccg atgccgatca cgatcacgag caacaggaag ttcttgatca ggtcggaacc
     1861 ggtgcgaccc accaggacgg ccgagcgcgc catgggcagg gagcggaagc ggtcgatgat
     1921 gcccgtcttc aggtcctcgg cgagaccgat cgcggtgttc gtcgattgga acaacgcgtt
     1981 ctggatgaag atgccgggca tcaggaactg cacgtagctc agttccggcg gcagctggga
     2041 gaactggttg gcgtagatga accggaacag cagcacgaac atgatcggct gcaccagctc
     2101 gaacacgaag accgtgggga ccctggtgag cgtgacgagg ttgcggcggg cgaccaggag
     2161 tccgtcgtcg aaggccaacg cgatgcgccc ggccgtgttg cggggcgcgc gccgttgcgt
     2221 gggggcttgc tggaccgtgg cgctcatgct tccttccctc ctcggcggcc gcgcttctcc
     2281 ttcttgccgt ccttgccgtc ggcttccgct tcgtcctcga cggccgaacg gcccgtgagt
     2341 ttgaggaaga cgtcgtcgag ggtggggcgg tgcagcgcga tgtccgcgag ccggatgtcg
     2401 gtttcgtcga ggcgtcgcac gctctccagg agggcgaccg gcccttcgcc acccacgggg
     2461 atgcgtaccg caccttcgtc ggcgtccacg gacggatcgc cggtaccgat gcccttgagg
     2521 gcctcgacca cccgtgaggt gtcgtcccgg ttctcgacgt ggagctcgag cacctcgccg
     2581 ccgacctgct ccttcaactc gtccgacgtg ccctgggcga tcagggcacc gcgatcgatc
     2641 acggcgatct ggtcggccag cacgtcggcc tcttcgaggt attgcgtggt caggagcagg
     2701 gtggtgcctt cgctctgcag ctcacggatg aagtcccaca cgtcgatccg gctgcggggg
     2761 tcgagtcccg tggtgggctc gtccaggaag agcacgtcgg ggcggccgac gaggctggcg
     2821 gcgaggtcga gcctgcggcg catgccgccg gagtaggtct tcgtggcgcg atccgccgcg
     2881 tcggccagat cgaaccgctc gaggacctcg acggcgcgtt gcttggcgag gcccttttcg
     2941 aggtgataga gccggccgac cagttggagg ttctcgagcc cggtcaggtt ctcgtcgacc
     3001 gcggcgctct ggcccgcgag gccgatccgg aaccggagct cttcggcatc cttgaccaca
     3061 tcgaagccgt cgacctcagc ccggcctccg gtgggaagca gcagggtggc caggatacgg
     3121 accatcgtgg tcttgccggc gccgttcggg ccgaggagcc cgagcacggt gcccttctcg
     3181 acctcgaggt cgaccccttt cagcgcgtgg acctcgccga agctcttcgt cacctgttcg
     3241 gccctgatga tggcaggcat ggtgcggcat cctacgaccc tcctctgaca agcgtcagcc
     3301 ctgcgcaggc ccctcaacgt ggcggacttg cggcttcttg cggtctcctt cccatgccgg
     3361 accgttgccc gtagtttcgt cccgtgcagc gcatcctcac cgtgatcgcc atcacgttcc
     3421 tgctcgttgt ctccgcctgc ggcggcgggg aggacggagc ctccccaacc accaccggcg
     3481 ccgcttcggc gcctcccacc gacacggcaa gcgccacccc gcagcccacg atcgaggcga
     3541 cggtccctgt cggcggcgaa ccgatcggga tctcggcggg ggagggcgcg atctgggtcg
     3601 tgaactccga gttcgagtcc ggcggcgaac cgtcggtctc gcgcatcgac cccgccacgt
     3661 cgaaggtcac agacacgatc gcggtggggg ccctgccgct cgaggtgctg gcggccggcg
     3721 ggtcggtctg ggtgtccaac tccgaggacg gcaccgtctc gcgcatcgac ccttcgagcg
     3781 cgaaggtcac cgccacgatc gaggtctgca aggcgcctga agggctggcg ctcgcggcgg
     3841 gttccgtctg ggtcgtctgc gaggagtccg acgccgtggg ccgcatcgat ccggccaccg
     3901 acacgatggt ggacaccgtg aaggtcgagg acgagccgcg gttcgccgcc gcggccttcg
     3961 gcagcgtgtg ggtgtccaac tacctggggt ccagcgtctc gcgcgtcgac cccgcgagcg
     4021 ggaaggtcac cgccaccatc gacgtcgacc tgggcggtca agtgatggcc gccctggacg
     4081 gttcgctctg gatctcctcg atcgactaca acacgttgac ccgcatcgat ccggagaccg
     4141 agaaggtcga gaaggtgttc aacgacgtcg gggactatcc cgacggcatc ctggcgagcc
     4201 agggcgcgct gtggatcgcc tccgacctgg gcccggagct gcggcgcttc gaccccgcca
     4261 ccggcaaggt gtccggcacg tgggtgatcg gagaacaggg gacgatcaac gcgaaccagc
     4321 tcctggctga ggccgacggc tccttctggc tgccgctgct ggagtcgagc gaggtcgccc
     4381 gggttgcgat ccccgactga ggggctcagg cttcggcggg cgggggcagc cggcgcaccg
     4441 ccgccaccaa cgatcgccgg tggcgttcca ccgcctcgac cgcgtcgttc gcgagggtgg
     4501 ccgcgccgta gcgcacgtcc tcgtaggccc tggtgagcgc gccgaaatcc tcggccacga
     4561 cgggatggcg ggccgacacc tcacgggcga actcggcggg cgtggcatcc ggcggcttcg
     4621 gctcgcccct ggcttcgagg gcatccaggg cgtcggcgta ggcgcgccgt accgcgtcgg
     4681 ccgacggcgt tcgacgggcc gcccggctcg gtggttcccc ggtgggccgg tcgtcggcgg
     4741 gcagcaccgt ggccgaggcg tcggcgggag catcgagcgg cgagatcgcg cgcgatggct
     4801 tcgcccggaa ccggcggaag agcatgatcg cgacggccgt gatccccacc aggaagagga
     4861 accccacgac gcgggcgaac ccaccaccgc caccgtgggg atcggcgcga tcggcggcgg
     4921 cgcggctggc ctcctggctg ctgcgacgga tccgctccaa caggtcggcg gcgccctggg
     4981 tgtcgaggtc ggcccgctcc aacgcccaga agatcgggcg ggcgagctgg gccaccgccc
     5041 acgccagcgc cccgacgacg gcgcggccca ccggcgcgac ccagctggcc agccggtcga
     5101 tgctgccggc ggtgaggacg gccacgagcg cgaccgcgcc gagtccgatc gcgagcctgc
     5161 cgctgtgacg gatccacgac gacgc
//
LOCUS       DATDBZ010000501         4868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_791779, whole genome shotgun sequence.
ACCESSION   DATDBZ010000501 DATDBZ010000000
VERSION     DATDBZ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791779"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VIX62_11750"
     CDS             <1..769
                     /locus_tag="VIX62_11750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5860950.1"
                     /translation="AKRRRDLTIGLVAGGIALILVAVGAFILFGGGDEQTDAASPSVS
                     AEPGTQTGTVTPQAVAPETVACGGKVPPKANKPKPQFAGPPPLMIDPSVTYTATIETS
                     CGTIEAELLAKTATDAVNSFVFLAQQGFYDGQTFHRIAKEFVIQGGDPLGTGTGGPGY
                     EFPVTTTEGVSFDGPGVLAYAHAQTGGNGSQFFITLAEAPNLDPPNGQYTIFGSVTKG
                     QDVLDAIAAVPTVAGPSGEPSQPTEAVYIDSVTIAES"
     gene            complement(774..2099)
                     /locus_tag="VIX62_11755"
     CDS             complement(774..2099)
                     /locus_tag="VIX62_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY5860951.1"
                     /translation="MPTDLTELHVDPLDALAAKIRERQATAAVVGLGYVGLPLLVGIH
                     QAGFASLGIDTDQTKIDVLTARRSHVVDVADSEIEAMDRATFSANPGPLGDADVIILC
                     LPTPLRDGAPDLTMVLTAAEDVARYLKPGVLVVLESTTYPGTTEELLRPILEGSGLVA
                     GRDFALGYSPERIDPGNRDHGLDSTPKIVAGLTDRCRELAVSFYSAFVHTVVTTTSPR
                     EAEMAKLIENTYRQVNIALVNELAVLARDLGVDIWESLQAASTKPFGYQAFWPGPGVG
                     GHCISIDPTYLSWRAGQQLGYRVEFIEHANEVNNRMPDYVVSRIAEALNDAGKPINGS
                     KVLGIGVAFKPGVDDLRGSPSLGVLERLQRKGAVVSYHDSFVDRCVIGDHEQGSLPLD
                     PETIGTQDLVVILTPHPEVDIHALVNTAAMVFDTRGVTVGMDSPNVVRL"
     gene            complement(2186..3190)
                     /gene="add"
                     /locus_tag="VIX62_11760"
     CDS             complement(2186..3190)
                     /gene="add"
                     /locus_tag="VIX62_11760"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY5860952.1"
                     /translation="MRDLRALPKAELHIHMEGAMRVATVLDLVERNGVPVPSGLTETG
                     WRFSGPGDFIAQYIAVCGLMTRLEDYQRLGREVAEDLSATGVRYAEAVFTPSAHAATM
                     GEDWFGPIEALLDGLAAGERATGTAVRLTPDVVRDAGMDAGRRTLEVARKFAGRGVVA
                     LNCAGSEVAAIAPFASLFAEAKEAGLGSVPHAGEWAGPENVWETLEHYAPDRIGHGVR
                     AIDDPQLVETLAERRIPLEVSPLSNVATGVYPSLEAHPFLRLRSAGVVVTLNSDDPPM
                     FGGGWLTPVYEAARRAWGLSDEELAAIARTGVDVSFADDERKAELGRGIEAWLADPGA
                     "
     gene            3214..3846
                     /locus_tag="VIX62_11765"
     CDS             3214..3846
                     /locus_tag="VIX62_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860953.1"
                     /translation="MHRARGIVRSSVIVCSMLLASCTVHLGSDQTASSAPGSFHRQDL
                     DAIVLTPDDAPPGTGYAEALSGFQDLDAFAKDAQERSDLVDDGFVVGHVSLFGPAGWS
                     GPGSGELPLDAPFAQGITGLFTTADGAASAYGRFGQDLRTRQFDRSEELPAPDLGQES
                     FALEGTSGGSHLVIVVWRDDNLVLAVSGSGVLDADDVRALASLVRARADR"
     gene            3905..>4868
                     /locus_tag="VIX62_11770"
     CDS             3905..>4868
                     /locus_tag="VIX62_11770"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5860954.1"
                     /translation="MEGTHPRVPMGEPGHRQVLGRGDLPLGDRCLVTGGAGFVGSNLV
                     HALVRQGMEVRVFDDLSTGSLVNLEDLVGEAEFVQGDVRDLEAVWAAAQGCTTVFHLA
                     AMTSAERAALDPGYAHGVNATGTHHVLTAARDAGCLRVIVASSAAIYGVQDGLPLHEE
                     LLPRPSTVYAATRIAAEASAVAFHAQYDLATVIVRPFNVFGPRQDFDAPDASVVARFI
                     VAALEGAPMTIDGDGEQSRDFVYVGDVVQAMLLAVDSTPDAWGRAYNAALGDRHTVNA
                     LADLVRRIVPGDQPSPVHAPARAGEIGASHADVRAAERVLGYSPE"
BASE COUNT          735 a         1670 c         1696 g          767 t
ORIGIN      
        1 cgccaagcgg cgccgcgatc tcacgatcgg gctcgtcgcc ggcgggatcg cgttgatcct
       61 ggtggcggtc ggcgcgttca tcctgttcgg gggcggcgat gaacagaccg acgccgcgag
      121 cccttcggtg tcggccgagc caggaaccca gacgggcacg gtcaccccgc aggcggtcgc
      181 gcccgagacg gtcgcctgcg gcgggaaagt gccgccgaag gccaacaagc ccaagccaca
      241 gttcgcgggg ccgccgccgc tcatgatcga tccctccgtc acctacacgg ccacgatcga
      301 gacctcgtgc ggcacgatcg aggccgagct gttggccaag acggcgaccg acgcggtcaa
      361 cagcttcgtc ttcctggcgc aacagggctt ctatgacggc cagaccttcc accgcatcgc
      421 caaggagttc gtgatccagg gcggcgaccc gctcgggacc ggcacgggtg gcccgggata
      481 cgaattcccc gtcacgacga cggaaggggt cagcttcgat ggacccggcg tgctcgccta
      541 tgcgcatgca cagaccggag gcaacgggag tcagttcttc atcacgctgg cggaggcgcc
      601 aaacctcgat ccacccaacg gccagtacac gatcttcgga agcgtcacga aggggcagga
      661 tgtgctcgat gcgatcgcgg ccgtgcccac ggtggcgggt ccttccggcg aaccgagcca
      721 gccgacggag gctgtctaca tcgactcggt gacgatcgcc gagtcctagg cgtttacagc
      781 cggaccacgt tcggcgaatc catccccacc gtgacccctc gggtgtcgaa caccatcgcc
      841 gcggtgttga ccagcgcgtg gatgtccacc tccggatggg gggtcaagat caccaccagg
      901 tcctgtgtgc cgatcgtctc ggggtccagc ggcaacgagc cctgttcgtg gtcgccgatc
      961 acacatcggt ccacgaagga gtcgtgatac gagaccaccg cgcccttgcg ctggagccgc
     1021 tcgagcaccc cgagcgaggg cgacccgcgc agatcgtcca cccccggttt gaacgccaca
     1081 ccgatgccca gcactttcga gccgttgatc ggcttgccgg cgtcgttcag cgcttcggcg
     1141 atccgcgaca ccacgtagtc gggcatgcgg ttgttgacct cgttcgcatg ctcgatgaac
     1201 tcgacgcgat agcccagttg ctggccggcg cgccacgaca gatacgtggg atcgatcgag
     1261 atgcagtgtc cgcccacgcc cggacccggc cagaaggcct ggtagccgaa cggcttcgtc
     1321 gaggcggcct gcagcgattc ccagatgtcg acgcccaggt cgcgggccag cacggccagc
     1381 tcgttcacca gggcgatgtt gacctgacgg taggtgttct cgatcagctt ggccatctcg
     1441 gcctcgcgcg gcgaggtcgt cgtcaccacg gtgtgcacga acgccgagta gaacgagacc
     1501 gcgagctctc ggcagcggtc ggtgagcccg gccacgatct tcggggtgga gtccaatccg
     1561 tggtcgcggt tgccaggatc gatgcgctcg ggtgagtacc ccagggcgaa atcgcgaccg
     1621 gccaccaggc cggatccctc caggatggga cgcaggagct cctcggtggt gccgggatag
     1681 gtcgtggatt ccagcaccac cagaacgccc ggcttgaggt agcgcgcgac gtcttcggcg
     1741 gcggtcagga ccatcgtgag gtccggcgcc ccgtcgcgca gcggggtggg cagacacagg
     1801 atgatgacgt cggcatcgcc cagcggcccc gggttggcgc tgaacgtggc tcggtccatg
     1861 gcttcgatct cggaatcggc gacgtcgacc acatgcgatc gccgggcggt caacacatcg
     1921 atcttcgtct ggtcggtgtc gatcccgagg gaggcgaacc ccgcttgatg gatacccacc
     1981 agcaacggca gacccacgta gccgagcccc accaccgcgg cggtggcttg gcgttctcgg
     2041 atcttcgcgg cgagcgcgtc gagtggatcc acgtgcaact cggtgaggtc ggtgggcatg
     2101 tcctcgttcg ctatcggccg ggaagacgcc gcccttcact ccgggggacg cgtgcgttcc
     2161 gagcctaggc gatgtccgcc cggcatcaag cacccggatc ggcgagccag gcctcgatcc
     2221 ctcggcccag ctcggccttg cgctcgtcgt cagcgaacga gacgtccacc cctgtccggg
     2281 cgatcgcggc gagctcctcg tcgctcaggc cccatgcccg gcgggcggcc tcgtacaccg
     2341 gggtgagcca tccgccgccg aacatcggcg gatcgtcgga gttgagcgtc accaccacgc
     2401 ctgcgcttcg caaccgtagg aatggatggg cctcgagcga ggggtagacg ccggtggcca
     2461 cgttggacag tggcgagacc tccaaaggga tccgtcgttc ggcgagcgtc tccaccagtt
     2521 ggggatcgtc gatcgcacga acgccgtgtc cgatgcggtc cggcgcgtag tgctcgagcg
     2581 tctcccacac gttctcgggc ccggcccatt cgccggcgtg cggaaccgaa cccaggccgg
     2641 cctccttggc ttcggcgaac agtgacgcga acggggcgat cgccgccacc tcgctgcccg
     2701 cacagttgag cgccaccacg ccgcggccgg cgaacttccg cgcgacctcg agcgtgcggc
     2761 gtcccgcgtc catcccggca tcgcgcacca cgtccggggt gagccggacc gcggtaccgg
     2821 tcgcgcgttc gccggcggcg agcccgtcca gcagtgcctc gatgggcccg aaccagtcct
     2881 cgcccatcgt ggcggcatgc gccgacgggg tgaagacggc ctcggcgtag cgcaccccgg
     2941 tggcggagag gtcctcggcg acctcgcggc ccagacgctg gtaatcctcc agccgcgtca
     3001 tcaggccgca cacggcgatg tactgcgcga tgaaatcgcc gggcccgctg aaccgccagc
     3061 cggtctcggt gaggcccgac ggtaccggca cgccgttgcg ctcgacgagg tcgagcacgg
     3121 tggcgacccg catcgctccc tccatgtgga tgtggagttc ggccttgggg agcgcacgga
     3181 ggtctcgcat ccacggatgg tagcgtcggc tccatgcacc gcgcccgcgg gatcgttcgg
     3241 tcgagcgtca tcgtgtgctc gatgctcctg gcctcgtgca cggtgcacct gggatccgac
     3301 cagacggcgt cgagcgctcc ggggtcgttc caccgccagg acctggacgc gatcgtgctc
     3361 acccccgacg atgcgccgcc cggcacgggc tacgccgagg cgctgtccgg gttccaggac
     3421 ctggacgcgt tcgcgaagga cgcacaggag cggagcgacc tcgtcgacga cgggttcgtg
     3481 gtgggtcacg tgtcgctgtt cggtcccgcg gggtggtcgg gcccggggtc gggagaactg
     3541 ccgctcgacg cgccgttcgc ccagggcatc accgggttgt tcacgaccgc cgacggcgcc
     3601 gccagtgcct acgggcgctt cggccaggat ctgcggacgc gacagttcga ccgttccgag
     3661 gagctccccg cgcccgacct gggccaggag tccttcgcgt tggaggggac ctctggcggg
     3721 tcgcatctgg tgatcgtcgt gtggcgtgac gacaacctgg tcttggccgt gtccggttcg
     3781 ggcgtgctgg acgccgacga cgtgcgggcg ctcgcctcgc tcgtccgcgc ccgcgccgac
     3841 cgatgaacgc acgtcgccag atcggatccc gttccggcgg cggagtgggc taggctcgcc
     3901 cagcatggaa ggcacccacc cgcgtgtccc catgggcgag ccggggcacc gccaggtgct
     3961 cggtcgggga gacctgccgc tgggcgatcg ttgcctcgtc acgggtggtg ccggcttcgt
     4021 gggatcgaac ctggtgcatg cgctcgtccg gcagggcatg gaggtccggg tcttcgacga
     4081 tctgtccacc ggctcgctcg tgaacctgga agacctcgtc ggggaagcgg agttcgtcca
     4141 aggtgacgtc cgcgacctgg aagccgtgtg ggcggcagcg cagggatgta cgacggtgtt
     4201 ccacctggcg gcgatgacct ccgccgaacg ggcggccctc gacccaggct acgcccacgg
     4261 cgtgaacgcc acgggcaccc accatgtgct cacggcagcg cgtgacgcgg ggtgcctgcg
     4321 ggtgatcgtg gcgtcgtccg ccgcgatcta cggcgtgcag gacggtctcc cgctccatga
     4381 agaactcctg ccgcgtccgt cgacggtcta cgcggccacc cgcatcgctg ccgaggcttc
     4441 cgccgtcgcc ttccacgcgc agtacgacct cgccaccgtc atcgtgcgcc cgttcaacgt
     4501 gttcgggccg cggcaggatt tcgatgcgcc cgatgcctcg gtggtggcgc ggttcatcgt
     4561 ggcggcgttg gagggtgcgc cgatgacgat cgacggggac ggggagcaat cacgcgattt
     4621 cgtgtacgtg ggcgacgtcg tgcaggcgat gttgttggcg gtcgactcga cgcccgatgc
     4681 ctgggggcgg gcgtacaacg cggccctcgg tgatcggcac acggtgaatg ccctcgccga
     4741 cctcgtgaga cggatcgtgc cgggggatca gccatcaccc gtccacgcgc ccgcgcgcgc
     4801 cggggagatc ggtgcatcac acgcggacgt ccgggcagcg gaacgggtcc tggggtattc
     4861 gccggaat
//
LOCUS       DATDBZ010000502         2112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1811783, whole genome shotgun sequence.
ACCESSION   DATDBZ010000502 DATDBZ010000000
VERSION     DATDBZ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811783"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VIX62_11775"
     CDS             complement(<1..814)
                     /locus_tag="VIX62_11775"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860955.1"
                     /translation="MTTDPNAAAQAAAEPRAPLSMTLGRFSALFLWVGFIILFGIITG
                     DTFFTQTTWKLTFSEGVVTAMLALAFLVPLVAGVYDLSVGAMMGLSLVMLNWFGANEP
                     GVPIALVAVLAIAACAAVGWLSAIIVVKFRVNSLIATLGMSQVLIAFQLKIADNRQIT
                     GAFSAGWAKLGTRDVVGIPIVVVYLLIVALVLWFTLEHTPVGRYLFAVGGNPDAARLA
                     GLNVERLTYGSLMASATIAGLAGVIFSMKVGTYSSSTGPGLLFPALAAVFFGA"
     gene            complement(855..>2112)
                     /locus_tag="VIX62_11780"
     CDS             complement(855..>2112)
                     /locus_tag="VIX62_11780"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY5860956.1"
                     /translation="VHQDLGLVNDLSITENVMLGRRYPRRRGGAINWKAARAAARDVI
                     GRAGSSLDVRRAIGELGIADRTRVAIARALPENDEPSIIVLDEPTAALPARDVEQLFN
                     TIRHLTEAGNAVVLVSHHLDEILGISDTITVLRDGVRVATVGVGEVDHDSLVQLIIGK
                     ELLKGGGRHEAVSHLGAPILTINELSADSVLDVNAVLHPGEIVGVAGLAGSGRELLGG
                     MVTGRLSRRGTITVAGVDVPAAQPKASLAAGMAWISGERARYGTFSNMNLRANLTMGD
                     LARHTRSGRVDVQSERAEVRDWIEKLGVVTRGPDVPITSLSGGNQQKVLVARALRLAP
                     RVLVLDDPTAGIDVGAREQVHAIIEQYTTESMAVLLVSTDSDELARLCDRVLVMVRGV
                     VAAELRRGIDLTAENIDHAQVAGIAA"
BASE COUNT          363 a          721 c          709 g          319 t
ORIGIN      
        1 acgcaccgaa gaagacggcc gcgagcgcag ggaagagcag cccgggcccg gtggacgagc
       61 tgtatgtgcc gaccttcatc gagaagatca caccggcaag gccggcgatc gtcgccgacg
      121 ccatcaagga gccatacgtc agtcgctcga cgttgaggcc ggcgaggcgg gccgcgtcgg
      181 ggttgccgcc gacagcaaag agatagcggc cgacaggcgt gtgctcgagg gtgaaccaca
      241 gcacgagcgc cacgatgagc aggtacacga ccacgatcgg gatcccgacc acgtctctcg
      301 tgcccagctt cgcccagccg gccgagaagg cgcccgtgat ctggcggttg tcggcgatct
      361 tcagctggaa cgcgatcagc acctgactca ttccgagcgt ggcgatcagc gagttgaccc
      421 ggaacttcac cacgatgatc gccgacagcc accccaccgc ggcgcacgcc gcgatggcga
      481 gcaccgccac gagcgcgatc ggcacacctg gttcgttggc gccgaaccag ttgagcatca
      541 cgagcgacag tcccatcatc gccccgacgg acaggtcgta gacccctgcc accaatggca
      601 cgaggaacgc gagcgcgagc attgcggtga cgacgccttc gctgaacgtc agcttccaag
      661 tggtctgtgt gaagaacgtg tcccccgtga tgatcccgaa gaggatgatg aagccgaccc
      721 agaggaacag cgcgctgaac ctgccgagtg tcatgctcag cggagctcgc ggctctgcgg
      781 cggcctgcgc cgcggcgttt ggatctgtgg tcatggtcat cggaggtacc ccaggtggat
      841 cgtgagcgcg gtagtcatgc ggcgattccc gccacttgcg cgtggtcgat gttctcggct
      901 gtcaggtcga tgccgcggcg cagctcggcc gccaccacgc cgcgcaccat cacgagcacc
      961 cgatcgcaga ggcgagcgag ctcgtcgctg tcggtcgaca ccagcaacac cgccatcgac
     1021 tccgtggtgt actgctcgat gatcgcgtgc acctgctcgc gagcgccgac gtcgatgccc
     1081 gccgtcgggt cgtcgagcac gagcaccctg ggggcgagcc gcaaggcacg ggcgacgagc
     1141 accttctgct ggttgccgcc tgacaggctg gtgatcggga catcaggtcc ccgggtcacg
     1201 acccccagct tctcgatcca gtcgcgcacc tccgcccgtt cggactggac gtcgacccgc
     1261 ccggagcgcg tgtgccgtgc caggtcgccc atcgtgagat tggcgcgcag gttcatgttg
     1321 ctgaacgtgc cgtagcgggc ccgttcgccc gatatccatg ccatcccggc cgccaacgac
     1381 gccttcggct gcgcagccgg aacgtccacc ccggccaccg tgatggtgcc gcgacgcgac
     1441 aaacgcccgg tgaccatgcc gccgaggagc tcgcgccccg agccggcgag cccggcgaca
     1501 ccgacgatct caccggggtg cagcacggcg ttcacgtcga gcaccgagtc ggccgagagc
     1561 tcgttgatag tgaggatcgg cgcgccgaga tgagagaccg cctcgtgccg cccaccgccc
     1621 ttcaggagtt ccttgccgat gatcagctgt acgagggaat cgtgatcgac ctcccccaca
     1681 ccgacggtcg ccacccgcac gccgtcgcgc aggaccgtga tcgtgtcgga gataccgagg
     1741 atctcgtcga ggtggtgcga cacgagcacg accgcgttgc cggcctcggt gaggtggcgg
     1801 atcgtgttga acagctgctc gacgtcgcga gcaggcagcg cggcggtggg ctcgtcgagc
     1861 acgatgatcg acggttcgtc gttctcaggg agtgctcgtg cgatggcgac gcgggttcga
     1921 tcggcgatgc ccagttcgcc gatcgcgcgc cggacgtcga gcgatgagcc cgcccggccg
     1981 atcacatcac gggcggcggc ccgtgccgcc ttccagttga tggccccgcc ccggcgccgc
     2041 gggtagcggc gcccgagcat gacgttctcg gtgatgctca gatcgttcac cagcccgagg
     2101 tcctggtgga cg
//
LOCUS       DATDBZ010000503         2582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1179783, whole genome shotgun sequence.
ACCESSION   DATDBZ010000503 DATDBZ010000000
VERSION     DATDBZ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179783"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VIX62_11785"
     CDS             <1..652
                     /locus_tag="VIX62_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5860957.1"
                     /translation="PPGEFIPLAEELGLIEAIGDWVIDAMAAQQRAWAADGLDLTVGF
                     NLSPRQLWSAHLAEKVLAKLRGADVDPERVVVEITESTAMADPDRTQKILNELHAWGL
                     TLAIDDFGTGYSSLARLKHLPVDILKIDRSFVRDVHLDPGLASMVQAMIQLAGGLGMT
                     PLAEGIETAGELAFLQEAGCPSGQGFYFARPVPADEIPDLVTREGGLIPPDFASDR"
     gene            1048..>2582
                     /gene="leuS"
                     /locus_tag="VIX62_11790"
     CDS             1048..>2582
                     /gene="leuS"
                     /locus_tag="VIX62_11790"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY5860958.1"
                     /translation="MTRRYDAAEIEPKWQQVWADEGLYRASEDPDDPRPRFYALDMFP
                     YPSGDLHMGHAEAFSGGDAVARYQSMRGYNVLHPIGWDAFGLPAENAAIKRGVPPKEW
                     TYANIEQQAASFQRMGMSFDWSRRLNTCDPSYYRWTQWLFTRFFAKGLAYRKNSPVNW
                     CPHDHTVLANEQVIQGACERCGTMVERRDLTQWFYKVTDYAQRLLDDMDDLIDWPERV
                     ITMQRNWIGRSEGARVTFTIAETGEDIEVYTTRPDTLWGVTFFVFAVDHPAVERLAEL
                     GGTADQVAPLREQARTTSLVDREAADTKDGVFLGVHAVNPVNGERIPVYAAPYVLMEY
                     GTGAIMAVPAHDQRDFEFARQHDLPVTVVIQPEGADPIDPATMTEALPHEGLMVNSGP
                     FDGEPSPASIAKVIAWLEAEGKGHAEVNFRLRDWLLSRQRFWGAPIPIIHCPTCGEVA
                     VPDDQLPVLLPDDADFTLGGESPLSRHPTWKHVQCPNCGGEAERDTDTMDTFVDSSWY
                     YYR"
BASE COUNT          404 a          877 c          887 g          414 t
ORIGIN      
        1 gccaccgggg gagttcatcc cgctcgcgga ggagctcggc ctgatcgagg cgatcggcga
       61 ttgggtgatc gacgcgatgg cggcccagca acgggcctgg gccgcggacg gcctcgacct
      121 cacggtcggg ttcaacctgt cgccgaggca gctgtggtcg gcgcacctgg ccgagaaggt
      181 cctggccaag ctgcgtgggg ccgacgtcga ccccgagcgg gtcgtggtgg agatcaccga
      241 gtcaacggcc atggccgacc ccgatcgcac ccagaagatc ctcaacgagc tgcacgcctg
      301 gggtctgacc ctggcgatcg atgacttcgg gaccgggtac tcgtcgctgg ctcgcctcaa
      361 gcacctgccg gtcgacatcc tcaagatcga tcgctcgttc gtccgggacg tgcacctcga
      421 ccccggcctc gcatccatgg tccaggcgat gatccagctg gccggcggcc tggggatgac
      481 cccgctcgcc gaagggatcg agaccgcggg tgaactcgcg ttcctccagg aggcgggatg
      541 cccctcgggt cagggcttct acttcgcccg cccggtgccc gccgacgaga tccccgatct
      601 ggtgacgcgc gagggtgggc tcatccctcc ggatttcgcc tccgatcgct gatcccgtgg
      661 ggagcgatcg ggactggggc cgcagaacga tccacggccc ctacgggcgc atgctgctct
      721 cgctcgccgc gacatcattg gtgctgatcg tggggttcct cgccagtccg ccgaccgcgc
      781 ccgacggcgc ggttccggct ccgggctcaa cgacgggtca acccgacgac gggttctcga
      841 gcgccacgct gagtgagtcc ggtgggtgct acttcgtccg gggcttcggg ctcccgacgg
      901 tcccacccga tccctcgccg gtggccgccg ttcacgggtc cacgtgccgg tgggtggagg
      961 tgacccgggc gcaactcgac agcctgatgg accggcgcgt ttgcgccttc ggcggttcct
     1021 cagtgcccgt tcgttaccat tgcgcgcatg accaggcggt acgacgcagc tgagatcgag
     1081 ccgaagtggc aacaggtgtg ggccgacgag ggcctgtacc gggcctccga ggaccccgac
     1141 gatccccgtc ctcggttcta cgcgctcgac atgttcccgt atccctcggg cgatctgcac
     1201 atgggccatg ccgaggcgtt cagcggcggc gacgcggtgg cccgctacca gtcgatgcgc
     1261 ggctacaacg tgctgcatcc gatcggctgg gacgccttcg ggctgccggc ggagaacgcg
     1321 gccatcaagc gtggcgttcc tccgaaggag tggacctacg ccaacatcga gcagcaggcg
     1381 gcctcgttcc agcgcatggg catgtcgttc gattggagcc ggcgcctcaa cacatgcgat
     1441 ccctcctact accgctggac ccagtggctg ttcacgcggt tcttcgccaa gggcctcgct
     1501 taccggaaga actcgccggt caattggtgt ccgcacgacc acacggtgct ggcgaacgaa
     1561 caggtgatcc agggggcgtg cgagcgctgc ggcacgatgg tggagcgccg tgacctgacg
     1621 cagtggttct acaaggtcac cgactacgcc cagcggctgt tggacgacat ggatgacctg
     1681 atcgactggc ccgagcgcgt catcacgatg cagcgcaact ggatcgggcg cagcgaagga
     1741 gcgcgtgtga ccttcacgat cgccgagacg ggcgaggaca tcgaggtcta cacgacgcgc
     1801 cccgacaccc tgtggggcgt cacgttcttc gtgttcgcgg tcgaccatcc ggcggtggag
     1861 cgcctggccg aactgggcgg cacggcagat caggtggcgc cgctgcgcga acaggcccgg
     1921 accacctcgc tggtggatcg cgaggccgcc gacaccaagg acggcgtgtt cctgggggtc
     1981 cacgccgtga acccggtgaa cggcgagcgg atcccggtgt acgccgcgcc ctacgtgttg
     2041 atggagtacg ggaccggcgc gatcatggcg gtgccggccc acgaccaacg cgacttcgag
     2101 ttcgcgcgcc agcacgacct gcccgtcacg gtcgtgatcc aacccgaggg cgccgatccg
     2161 atcgaccccg ccacgatgac cgaagcgctg ccgcacgagg ggctcatggt caacagcggt
     2221 ccgttcgacg gggaaccctc gccggcctcg atcgccaagg tgatcgcctg gctggaggcc
     2281 gaaggcaagg ggcatgccga ggtgaacttc cggctgcgcg attggttgct cagccgccag
     2341 cggttctggg gcgcgccgat cccgatcatc cattgcccca cgtgcggcga ggtcgcggtg
     2401 cccgacgacc aactgcccgt cctgctcccg gatgacgccg acttcacgct gggcggggag
     2461 tcgcccctgt cgcgtcaccc cacgtggaag cacgtgcagt gtccgaactg cggcggcgag
     2521 gccgagcgcg acaccgacac gatggacacg ttcgtggatt cgagttggta ctactaccgg
     2581 ta
//
LOCUS       DATDBZ010000504         2322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1611870, whole genome shotgun sequence.
ACCESSION   DATDBZ010000504 DATDBZ010000000
VERSION     DATDBZ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611870"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VIX62_11795"
     CDS             <1..385
                     /locus_tag="VIX62_11795"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HEY5860959.1"
                     /translation="DTAEEKQRSALASAALASSLGADGVICTTFSSGNSHTDTMLTVR
                     ACEAVGIATTALVCETNGGLTDHVPEADCLVSTGNEDELLEPWTPDRVIGSMNAARVG
                     EPVPTWAYVAACVETGDAAWTAVPA"
     gene            382..1668
                     /locus_tag="VIX62_11800"
     CDS             382..1668
                     /locus_tag="VIX62_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1402..1404,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HEY5860960.1"
                     /translation="MTIVHYVNQFFAGLGGEEAAGHEPVQLEGPQGPGRAFAAAGATP
                     DVTLACGDDFFGEHEAEALATMLAWLEELEPDVLMCGPSFGSGRYGYACGVLAREAGR
                     RGIPVVAAMTPDSPGVMAAEGAAYVVPTGSNVAAMRTVVPVMAGLATRLAAGEAVGSP
                     DEEGYLPRGLRSNVRSDRPGAVRAIDLLLAKLAGDVRTEVGPTGDRVPPAPPVADLAG
                     VRLALVTEAGCVPHGNPDQLPTRHANVWLRYPISDVDSLSAEAYESVHAGFDTTAANA
                     DPNRLVPLDAARALERRGSFGSLHDAFYTTSGVDTPVATAAKFGQEIAAELREAKVGA
                     VILTGTUGTGTRCGATLAKEFEREGITTVFVTALPTIAQMVGTNRILRGVSITSPTGD
                     PSLATGDEHELRVAIMERALEMLTTEVGPTTIWELT"
     gene            1665..>2322
                     /locus_tag="VIX62_11805"
     CDS             1665..>2322
                     /locus_tag="VIX62_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HEY5860961.1"
                     /translation="MTERRRAAVVSASFVLEHVPDLVRYGSKPAREPDQLDRLTGALR
                     SYEDAVAYPPNQVFVGNLRPEDLWEVPRTWWEHPVEGASPLGRFGDVMAQGDFYSLLA
                     DVDRFDLVRMGAEPAPGELALRSGDQVVGAFAGDHDLDESLTANVLLENLSIKASGVH
                     ALRDLLTRTDIDPSSITHAIGCGEEAVGDRYQRGGGNVAKAIAEECGLLRASAIDVKS
                     F"
BASE COUNT          356 a          781 c          817 g          368 t
ORIGIN      
        1 tgataccgcc gaagagaagc agcgatcggc actggcgtcg gcggcgctcg catcgtccct
       61 gggtgccgac ggcgtgatct gcacgacgtt ctcgtctggg aactcacaca cggacaccat
      121 gctcaccgta cgtgcgtgtg aagctgtcgg catcgcgacg acggcgttgg tgtgtgagac
      181 gaacggtgga ctcaccgacc atgtcccgga ggcggactgc ctcgtcagta ccggcaacga
      241 ggacgagctg ctcgagccct ggacacccga ccgcgtgatc ggctcgatga acgctgcccg
      301 cgtcggcgag ccggttccca cctgggccta cgtcgcggcg tgcgtcgaga ccggcgacgc
      361 cgcgtggacg gcggtgcccg catgacgatc gtccactacg tgaaccagtt cttcgccggg
      421 ctcggtggcg aggaggccgc tgggcacgag cccgtccagc tggaaggccc acagggtccc
      481 ggtcgtgcgt tcgcggcggc aggcgccacg cccgacgtca ccctcgcgtg tggcgatgac
      541 ttcttcggcg agcacgaagc ggaagcgctc gcgacgatgc tcgcctggct cgaggagctc
      601 gagcccgacg tgctgatgtg tggtccctcg ttcggatcgg ggcgctacgg gtatgcctgc
      661 ggcgtgctgg ctcgcgaagc gggccgtcgg gggatccctg tggtggccgc catgaccccg
      721 gactcgcccg gcgtgatggc cgccgaggga gccgcctacg tcgtgcccac cgggtcgaac
      781 gtagccgcga tgcgcaccgt cgtgccggtc atggccgggc tcgccacacg cctcgccgcc
      841 ggagaggcag tcgggagccc tgatgaagag ggctacctcc cgcgaggact tcgttcgaac
      901 gtccggtccg accggcccgg cgcggtccgc gcgatcgacc tgctgctggc caagctcgcc
      961 ggcgatgtgc ggaccgaggt cggtcccacc ggtgaccggg tgccgccggc gccaccggtc
     1021 gccgatctcg ccggcgttcg actcgcgctc gtcaccgagg ccgggtgcgt gccccacggc
     1081 aaccccgatc agctgccgac acgtcacgcg aacgtgtggt tgcgctaccc gatctccgat
     1141 gtcgattccc tgtcggcgga ggcgtacgag tccgtccacg caggcttcga cacgacggcg
     1201 gcgaacgccg atccgaaccg gctcgtcccg ctcgatgccg ctcgtgcgct cgaacgccgc
     1261 ggctcgttcg gctcgctcca cgacgcgttc tacaccacgt ccggtgtcga cacgccggtc
     1321 gcgacggccg ccaagttcgg tcaggagatc gccgcggaac tgcgcgaggc aaaggtcggg
     1381 gccgtcatcc tcaccggcac ctgaggtacg ggtacgcgtt gcggggcaac gcttgcaaag
     1441 gaattcgaga gggaagggat caccacggtg ttcgtcacgg cgctgcccac gatcgcgcag
     1501 atggtaggga cgaatcgcat cctgcgcggt gtctcgatca cgtcgcccac gggtgatccg
     1561 tcgctggcga ccggcgacga gcacgaactt cgcgttgcca tcatggaacg cgcgctcgag
     1621 atgctcacca ctgaggtcgg gccgacgacg atctgggaac tgacgtgacc gaacgacgcc
     1681 gggctgcggt ggtgtcagct tccttcgtgt tggagcacgt gcccgacctc gtgcggtacg
     1741 ggtcgaagcc ggcgcgcgaa cccgaccagt tggaccggct caccggggcg ctccggagct
     1801 acgaggacgc ggtcgcgtat ccaccgaacc aggtgttcgt gggcaacctc aggcccgaag
     1861 atctctggga ggttcctcgc acgtggtggg aacaccccgt ggagggcgcg tcgccgctgg
     1921 gtcggttcgg cgatgtcatg gcgcaagggg acttctactc gttgctcgcc gacgtcgacc
     1981 ggttcgacct ggtgcggatg ggcgccgaac ccgcaccggg tgaactcgcg ctccgctcgg
     2041 gcgatcaggt cgtgggagcc ttcgccggcg atcacgacct ggacgagtcg ctcaccgcga
     2101 acgtgctcct cgagaacctc tcgatcaagg cgagtggcgt gcatgcgctg cgcgacctgc
     2161 tcaccaggac cgacatcgat ccttcgtcga tcacgcatgc gatcggctgc ggtgaggagg
     2221 cggtcggtga ccgataccaa cgcggcggag gcaacgtcgc gaaggcgatc gccgaggagt
     2281 gcgggctcct gcgtgccagc gcgatcgacg tgaagtcatt ct
//
LOCUS       DATDBZ010000505         4659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1995173, whole genome shotgun sequence.
ACCESSION   DATDBZ010000505 DATDBZ010000000
VERSION     DATDBZ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995173"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1518)
                     /gene="gatA"
                     /locus_tag="VIX62_11810"
     CDS             complement(43..1518)
                     /gene="gatA"
                     /locus_tag="VIX62_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY5860962.1"
                     /translation="MSERELCDRTGTELSAQLRAGEITAVELTDSSLARLEAVNDRLG
                     AFLSVTTEVARERAAELDTYLATGAPQSGVAGIPTALKDVLTTKGIRTTCASKILESY
                     VPPYDCTAWTRLSGAGSVLVGKTNCDEFAMGSSTENSAYGVVHNPWNLDTVPGGSSGG
                     SAAAVAAGAAVWALGTDTGGSVRQPASLTGVVGLKPTYGLISRYGLVAFASSLDTVGT
                     FTRSVPDAATLLGVLAGKDHRDATSLDVEVPDYHEQLGRGVEGLRIGVLADAFGEGVE
                     PDVAASVRAAVDRLADLGAQVGEATLPHAGYALSAYYLIAPSEASSNLARYDGVRYGL
                     RVDGDDSIEMMFRTRGAGFGPEVKRRIMLGTYALSAGYYDAYYGQAQKVRTLIIRDYE
                     AAFADFDVLVSPTSPTTAFPIGAKVDDPMAMYLNDIFTIPANLAGVPAISVPCGLDST
                     GLPIGLQFTAPVLGEQALLRAAAALERELALALRPPLVAGV"
     gene            complement(1515..1808)
                     /gene="gatC"
                     /locus_tag="VIX62_11815"
     CDS             complement(1515..1808)
                     /gene="gatC"
                     /locus_tag="VIX62_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY5860963.1"
                     /translation="MAIEIDIDHVARLARLDLTEEEKTELRAQLGVILEHAAAVGEVA
                     ADDVPPTASAIPRANVFREDTPEPSIPHDAALANAPAQADGRFMVPKIAETGS"
     gene            complement(1843..2586)
                     /locus_tag="VIX62_11820"
     CDS             complement(1843..2586)
                     /locus_tag="VIX62_11820"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEY5860964.1"
                     /translation="METPARGKPLGVLTYWVVAMLAVTAVIDILGVVSLLIERSLVLD
                     SLAGRPVSIEEAVASDNRVEAIGGWTLIALLVTAVLWCIWQHRAHSNLRSFGRIGLSY
                     TPGWAVGWWFVPLASLWKPLGAMSELWKASEPTDDPKGWWRARAWWVLGLWWACWIGG
                     NLLDIVASAMQQESDLSSVASSDIVAVLARLVEIAAAICAVLILRDILGRQRALAPMS
                     GAAPLPPPLPDATVALPPMPPPPPLPPQP"
     gene            complement(2648..>4659)
                     /gene="ligA"
                     /locus_tag="VIX62_11825"
     CDS             complement(2648..>4659)
                     /gene="ligA"
                     /locus_tag="VIX62_11825"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY5860965.1"
                     /translation="ERERAEAKLRVEELRAEIEHHRYRYHVLDDPEVADAEYDVLMRE
                     LRALEDRFPELQTPDSPTVTVGGAPADLFAPVTHRAPMLSLDNAFSFEELDAWAARVE
                     RGVGDAARYACELKIDGVACALTYERGRLVKAATRGDGRIGEDITANVRTVKGIPRTL
                     TVEDPPAVVEIRGEMYFPVTAFEELNDQLLEAGGKAFSNPRNAAAGSLRQKDPKVTAS
                     RPLRLWVHSFGFAQGITFDSHLGFLEWAGSAGLPVAPTTESRDSIDGVKDFLQHWETN
                     RHSIDWEIDGTVVKVDQTDLQRELGNTSHAPRWAIAYKFPPEERTTLLKSIDVHTGRT
                     GKVTPFAVLDPVFVSGVTVTYATLHNEDEVRRKDVRKGDMVIVRRAGDVIPEIVGPVL
                     SKRKKGARRWSMPKTCPSCGTPLVRPEGEADYRCPNKRGCPSQGIEWLFHFSGRGAMD
                     IEHLGYMTVMRLLEAGLIEDPADIYALDADKLGRLPGFKDKSITNVLEQIELSKERPL
                     WRLLVGLNIRHVGSHVAQVLARAFGSIDALAAATADQIDDVPEIGPEIAATVAEWFAE
                     DENLALIEKLRAAGVSLADQRSGEDGPRLLEGLSIVLTGGMEEITREEATELAQAAGA
                     RVASSVSKKTSFVVAGENPGSKLAKAEGLGVEVIDEPEFLRRLGR"
BASE COUNT          704 a         1653 c         1588 g          714 t
ORIGIN      
        1 ggcaacccgg ccgcaggcgt cgcgcgcgag atggccgaag gctcagacgc cggcgacgag
       61 cggcggccgg agcgcgaggg cgagctcccg ctcgagagcg gcggccgccc ggagcagcgc
      121 ctgctcgcca aggacgggag cggtgaactg gagaccgatc gggagcccgg ttgagtcgag
      181 gccgcagggg acgctgatcg cgggcacgcc cgccaggttc gccgggatcg tgaagatgtc
      241 gttcaggtac atcgccatgg ggtcgtccac cttcgccccg atcgggaatg cggtggtggg
      301 cgaggtgggc gagaccagca cgtcgaagtc ggcgaacgcg gcttcgtaat cgcggatgat
      361 cagcgtccgc accttctgtg cctggccgta gtacgcgtcg tagtagccgg cgctcaacgc
      421 atacgtgccg agcatgatgc ggcgcttcac ctcggggccg aaccccgctc cgcgcgtgcg
      481 gaacatcatc tcgatcgagt cgtcgccgtc gacacgcagg ccgtatcgca ctccgtcgta
      541 ccgggccagg ttcgaggagg cctcgctagg cgcgatcagg tagtaggcgc tcagcgcgta
      601 cccggcgtgc ggaagggtcg cctcccccac ctgcgcgccg aggtcggcga gccgatcgac
      661 ggccgcccgc acggaggcgg cgacgtcggg ctcgacgccc tcgccgaacg cgtcggcgag
      721 cacgccgatc cgcagccctt ccacgccccg gccgagctgc tcgtggtagt cgggcacctc
      781 gacgtcgagg ctggtcgcgt cgcgatgatc cttgccggcg aggacgccga gcagggtggc
      841 ggcgtctggc acgctccttg tgaacgtgcc caccgtgtcc agcgacgagg cgaacgccac
      901 caacccgtag cggctgatca acccgtaggt gggcttgagg cctacgacgc cggtgagcga
      961 cgccggctgg cgcaccgagc cgccggtgtc ggtgcccagg gcccacaccg cggcgcccgc
     1021 ggccaccgcg gccgcgctcc cgccgctcga gccgcccggc accgtgtcca ggttccacgg
     1081 attgtgcacg accccgtagg cagagttctc ggtggacgag cccatcgcga actcgtcgca
     1141 gttggtcttg cccaccagca cgctgcctgc gcccgacagc cgcgtccacg cggtgcagtc
     1201 atagggcggg acgtagctct ccaggatctt cgacgcgcac gtcgttcgga tgcccttggt
     1261 ggtgagcacg tctttgaggg cggtggggat gccggccacg cccgattgcg gcgcgccggt
     1321 ggcgaggtag gtgtcgagtt cggccgcgcg ttcgcgcgcc acctccgtgg tcacggacag
     1381 gaacgcgccg agccggtcgt tgaccgcttc gagccgggcc aggctggaat cggtgagctc
     1441 caccgcggtg atctcgccgg cgcggagctg ggccgagagc tcggtcccgg tgcgatcgca
     1501 gagctcgcgc tcgctcacga gccggtctcc gcgatcttcg gcaccatgaa ccggccgtcg
     1561 gcctgtgccg gagcgttggc cagtgccgcg tcgtgcggga tcgacggctc gggcgtgtcc
     1621 tcgcggaaga cgttcgcccg tgggatcgct gaagcggtgg gaggcacgtc atcggccgcc
     1681 acctcgccca cagcggcggc gtgctcgagg atgaccccga gctgcgcacg caactcggtc
     1741 ttctcctcct cggtcagatc cagccgggcg agtcgtgcga cgtggtcgat gtcgatctct
     1801 atcgccatgg cgggcagtgt agcggccgac gttcagacga ggtcagggct gcgggggcaa
     1861 gggtggtgga ggtggcatcg gaggcagcgc caccgtggcg tcgggcaggg gcgggggcaa
     1921 cggcgccgcc cccgacatcg gcgcgagcgc gcgctgccgg ccgaggatgt cccgcaggat
     1981 caggaccgcg cagatggcgg cagcgatctc caccaggcgc gcgagcaccg cgacgatgtc
     2041 gctcgatgcc acggagctga gatcgctctc ctgctgcatc gcgctcgcca cgatatccag
     2101 gaggttcccc ccgatccagc acgcccacca cagccccagc acccaccagg ccctggcacg
     2161 ccaccaaccc ttgggatcgt cggtgggttc gctggctttc cagagctccg acatcgctcc
     2221 caagggcttc cagaggctcg ccaacgggac gaaccaccag cccacggccc atccgggtgt
     2281 gtagctcaga ccgatgcgcc cgaacgaccg gaggttgctg tgggcccggt gctgccagat
     2341 gcaccacagc accgcggtga cgagcagcgc gatcaacgtc cagccaccga tcgcctcgac
     2401 acggttgtcg ctcgcaaccg cttcctcgat cgacaccgga cgtccggcga gcgaatcgag
     2461 caccagcgaa cgctcgatca acagcgacac cacaccgagg atgtcgatca cggccgtcac
     2521 cgccagcatg gccaccaccc agtacgtgag gaccccgagc ggtttgccgc gcgccggtgt
     2581 ctccatggcg aaaccctacg atgcctgcgc cggcgtttcg accaaccttc gtcagaacca
     2641 ggacggctca gcgccccaga cgacggagga actcgggttc atcgatcacc tcgaccccga
     2701 gcccttcagc cttcgcgagc ttggagcccg ggttctcgcc ggcgaccacg aacgaggtct
     2761 tcttcgagac gctggaggcg acccgggcgc ccgcggcctg cgcgagctcg gtggcttcct
     2821 cgcgcgtgat ctcctccatc cccccggtca gcacgatcga gagcccttcg agcagcctgg
     2881 ggccgtcctc gcccgatcgc tggtcggcca ggctcacgcc cgccgcgcgg agcttctcga
     2941 tcagcgccag gttctcgtcc tcggcgaacc actcggccac ggttgccgcg atctcgggtc
     3001 cgatctcggg cacatcgtcg atctgatcgg cggtggccgc agctagggca tcgatcgaac
     3061 cgaacgcgcg ggcgagcacc tgggccacgt gcgagccgac gtgccggatg ttcaggccca
     3121 ccagcagccg ccacagcggt cgttccttcg agagctcgat ctgttccagc acgttggtga
     3181 tcgacttgtc cttgaagccc ggcagccggc ccagcttgtc ggcatcgagc gcgtagatat
     3241 ccgcggggtc ttcgatcagc ccggcctcga gcagccgcat caccgtcatg tagcccaggt
     3301 gctcgatgtc catggcgccg cggccggaga aatggaacaa ccactcgatg ccctgcgagg
     3361 ggcacccgcg cttgttcggg cagcgatagt cggcctcgcc ttccggacgc accaacggcg
     3421 tgccgcagga cgggcaggtc ttcggcatcg accatcgccg agcgcccttc ttccgcttgg
     3481 agagcaccgg cccgacgatc tcggggatca cgtcgcccgc gcggcgcacg atcaccatgt
     3541 cgcccttgcg cacgtccttg cggcggacct cgtcctcgtt gtgcaacgtg gcgtaggtca
     3601 ccgtgacccc gctgacgaac acgggatcga gcacggcgaa cggcgtgacc ttgcccgtgc
     3661 gtcccgtgtg cacgtcgatc gatttcagca gcgtggtgcg ttcctcgggt gggaacttgt
     3721 aagcgatcgc ccaccggggg gcgtggctcg tgttccccag ttcgcgctgc agatcggtct
     3781 gatcgacctt gaccacggtg ccgtcgatct cccagtcgat cgagtgccgg ttcgtctccc
     3841 agtgctgcag gaagtccttg accccgtcga tcgaatcgcg agactcggtg gtaggcgcca
     3901 ccggcaaccc cgccgagccc gcccattcga ggaaccccag gtgcgagtcg aacgtgatgc
     3961 cctgcgcgaa gccgaacgaa tgcacccaca ggcgcagcgg ccgcgacgcc gtgaccttcg
     4021 ggtccttctg ccgcagcgac ccggccgcgg cgttccgcgg gttcgagaac gccttgccgc
     4081 cggcttccaa cagctggtcg ttcagctcct cgaacgccgt caccgggaag tacatctcgc
     4141 cgcggatctc gaccaccgcg ggcggatcct cgacggtcag ggtgcggggg atgcccttca
     4201 ccgtgcggac gttcgcggtg atgtcttccc cgatgcggcc gtcaccgcgg gtcgcggcct
     4261 tcaccaggcg cccgcgctca taggtgaggg cgcacgcgac gccgtcgatc ttcagctcgc
     4321 aggcgtaccg ggcggcatcg cccacgccgc gctccacccg ggccgcccac gcgtcgagct
     4381 cctcgaagct gaaggcgttg tcgagcgaga gcatcggcgc gcgatgcgtg accggcgcga
     4441 acaggtcggc gggcgcgccg cccacggtga cggtgggtga gtcgggcgtc tgcaattcgg
     4501 ggaaccggtc ctccagtgcc cggagttcgc gcatcagcac gtcgtactcg gcgtcagcca
     4561 cctccggatc gtcgagcacg tgataccgat atcggtggtg ctcgatctcg gcgcggagct
     4621 cctcgacgcg cagcttggcc tcggcgcgct cacgctcgt
//
LOCUS       DATDBZ010000506         3007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_991975, whole genome shotgun sequence.
ACCESSION   DATDBZ010000506 DATDBZ010000000
VERSION     DATDBZ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991975"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VIX62_11830"
     CDS             <1..353
                     /locus_tag="VIX62_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5860966.1"
                     /translation="LSFGQRRRAAVATVLSMRPDLLVLDEPSSNLDPAARRELAEILV
                     SLPITTLIVTHDLPYALQLCPRSIVLNNGRVAAAGPTRSILADADVMMANRLELPFGF
                     TLSPADDAVSAGDG"
     gene            423..1070
                     /locus_tag="VIX62_11835"
     CDS             423..1070
                     /locus_tag="VIX62_11835"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY5860967.1"
                     /translation="MRKYVIMGIQGSGKGTQAKLLADDFDLVQLSIGDILRWNIQHHT
                     KLGANVRRIVASGLLVDDELIERVMQERLALHDWNFGFIIDGFPRNERQTEFFLESYD
                     IDGVINLELPDAEVERRVLARRLCSGCGLDYNLIAHRPKVEDVCDVCGSPLVVRADDN
                     PDALSVRLREYREKTLPVIELLQRKEFVATIDATRSVPEIQAEIRARFGLRVPAP"
     gene            complement(1137..2135)
                     /locus_tag="VIX62_11840"
     CDS             complement(1137..2135)
                     /locus_tag="VIX62_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY5860968.1"
                     /translation="MHVWSVDQPGPLEERPLRRMHRAVPSPGPFEVRVRVAACGVCRT
                     DLHIVAGDLPPHRRGVVPGHEVVGYVDAVGADATRFAEGERIGIPWLRSTCGTCRFCS
                     TGRENLCLDPRFTGWDDDGGYAEYAVVPEAFAYRLPDQFDDITAAPLLCAGIIGYRAL
                     ERALVPPNGRLGIYGFGGSAHLTAQLAIHRGATVHVLTRSAAARALALDFGCASAGEA
                     TEMPPEPLDSAILFAPAGELVPVALRALDRGGTLAIAGIHLSAIPTLDYDGALFQERQ
                     VRSVTANTRVDGERFLAEAAVAAIRVATVEYRFEDADRALIDLWDDKVDGAAVLVA"
     gene            complement(2165..2623)
                     /locus_tag="VIX62_11845"
     CDS             complement(2165..2623)
                     /locus_tag="VIX62_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5860969.1"
                     /translation="MGDEFDIIVDVLDESTCWRLMSRSGFGRLGFMHGDELMVLPVNA
                     AVSENRVVFRTTDDSMFARVGDGTKVVFEVDQTDRVAESGWSVLLRGRLWDVTGRPET
                     ATWQELSVHPWAQGARDRWMAIEPTAVSGRRVLRHRTLPPGVHPSYMPPD"
     gene            2846..>3007
                     /locus_tag="VIX62_11850"
     CDS             2846..>3007
                     /locus_tag="VIX62_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5860970.1"
                     /translation="MSEHAVPLYEQVKAFIVNEIEPITEEFFRLGEGRADRWSYAPGQ
                     LELLDSVKAK"
BASE COUNT          510 a         1069 c          962 g          466 t
ORIGIN      
        1 acctcagctt cggccaacga cgccgagccg ccgtcgccac cgtgctgtcc atgcgccccg
       61 atctcctggt gctcgacgag ccctccagca acctcgaccc ggcggccaga cgagagctcg
      121 ccgagatcct cgtctcgctc ccgatcacga cgctgatcgt cacccacgat ctgccgtacg
      181 cactgcaact gtgcccgcgc tcgatcgtgc tcaacaacgg ccgtgtcgcc gccgctggtc
      241 cgacgcgctc gatcctggcc gacgccgacg tgatgatggc caaccgactc gagctgccgt
      301 tcggtttcac cctctcgccg gctgacgatg ccgtcagcgc aggagatgga tgaccgatcg
      361 cggaggcgat ccgcagttcc ggcgccgacg tggccagcgt tgcgatctgg caggctcggg
      421 ccgtgcgcaa gtacgtgatc atggggatcc aggggagcgg caagggcacc caggccaagc
      481 tgctggccga cgacttcgac ctcgttcaac tcagcatcgg cgacatcctc cggtggaaca
      541 tccagcacca caccaagctc ggcgccaacg tgaggcgcat cgtggcatcc ggcctcctgg
      601 tcgatgacga gctgatcgag cgggtgatgc aggagcggct ggcgctgcac gactggaact
      661 tcggcttcat catcgacggc ttcccgcgca acgagcgcca gaccgagttc ttcctcgaga
      721 gttacgacat cgacggcgtg atcaacctcg agctccccga cgccgaggtc gagcgccgcg
      781 tgctcgcccg ccggctctgc tcgggctgcg ggctcgacta caacctcatt gcccatcgcc
      841 cgaaggtcga ggatgtgtgc gacgtgtgcg gatcgccgct cgtcgtgcgg gccgacgaca
      901 atccggacgc gctctccgtc cggctgcgcg agtaccgcga gaagaccttg cccgtgatcg
      961 agctgctcca gcgcaaggag ttcgtcgcca cgatcgatgc gacccgttcg gtgcccgaga
     1021 tccaggctga gatccgggcc cgcttcggcc tgcgcgtccc ggcgccgtag gttcaggccg
     1081 ggacagcccc tgccccggcg gaaagctcga tcgtgcgaac agctgccatg tcctgctcag
     1141 gcgaccagca cggcggcgcc gtcgaccttg tcgtcccaca ggtcgatgag cgcacggtcc
     1201 gcatcctcga accgatactc gaccgtggcg acgcggatgg cggcgacagc tgcctccgcc
     1261 aggaaccgct caccgtcgac cctcgtgttg gcggtgacgc tcctgacctg ccgctcctgg
     1321 aacagcgccc cgtcatagtc gagcgtcggg atcgccgaca gatggatccc ggcgatcgcc
     1381 aaggtgccgc cacgatcgag ggcgcgcagc gcgaccggaa cgagctcgcc cgccggggcg
     1441 aacaggatcg ccgaatcgag cggctcgggc ggcatttcgg tcgcctcacc tgccgacgcg
     1501 cagccgaaat cgagcgccag ggctcgagca gcggcgctgc gggtcagcac gtgcaccgtg
     1561 gcaccgcgat ggatcgcgag ctgggctgtc aggtgggcgg atccaccgaa gccgtagatg
     1621 ccgagccgcc cgttgggcgg cacgagggcg cgttcgaggg cgcgataacc gatgatcccg
     1681 gcacacagga gcggggcggc cgtgatgtcg tcgaactgat ctggcagccg gtaggcgaac
     1741 gcttccggga caacggcgta ctccgcgtac ccgccgtcgt cgtcccatcc cgtgaacctc
     1801 ggatcgaggc acaggttctc ccgcccggtc gagcagaacc ggcaggtgcc acaggtgctc
     1861 cgtagccacg ggatgccgat ccgttcgcct tcggcgaacc gcgtcgcatc tgctccgacc
     1921 gcgtccacgt aacccaccac ctcatgccca ggcacgaccc cgcgtcgatg gggtggcagg
     1981 tcgccggcca cgatgtgcag gtcggtgcgg cacacaccac acgccgcaac gcggacgcgc
     2041 acctcgaacg gtccgggcga tgggaccgca cgatgcatcc gccggagcgg ccgttcctcg
     2101 agcgggcctg gctggtcgac ggaccacacg tgcatcatcg ggttcgccat ctcgccggtc
     2161 tcggtcaatc aggcggcatg tatgacggat gcacccccgg tggcagggtc cgatgacgga
     2221 ggaccctccg tccgctgact gcagtcggct cgatcgccat ccaccggtcc cgggctccct
     2281 gcgcccaggg gtgcacgctg agctcctgcc acgttgccgt ttccggccgg ccggtcacgt
     2341 cccacagccg gccacgcagg aggacgctcc agcccgactc ggccacccga tcggtctgat
     2401 ccacttcgaa cacgaccttc gtgccgtcgc cgacgcgggc gaacatcgag tcgtcggtgg
     2461 tgcggaacac cacgcgattc tcgctcaccg ccgcgttcac cggcagcacc atgagctcgt
     2521 cgccgtgcat gaagccgaga cgcccgaatc cgcttcgcga catgaggcgc cagcaggtcg
     2581 attcatccag cacgtcaacg atgatgtcga actcatcgcc catgacctgc ctcctcgttg
     2641 ctaccggcgt aattgcacga tgcatcgaag cctgaacaac catcagaggc aaaggtccca
     2701 gcgctcgcca gccgaggctc gaccggagcc cggacagcgt cggtcagtag tgtcgatttg
     2761 gatcgtccgc ccacgaaggg aagcactcgt gcccagtccc ggaatgcatt ccggcatcgc
     2821 caccgacgat gcgttgaacc tctcgatgtc cgaacacgcc gtaccgctgt acgagcaggt
     2881 gaaggcgttc atcgtcaacg agatcgagcc gatcaccgag gagttcttcc gactcggtga
     2941 gggccgggcg gatcgctggt cgtatgcgcc ggggcagctg gaactgctcg actccgtgaa
     3001 ggccaag
//
LOCUS       DATDBZ010000507         3917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2624969, whole genome shotgun sequence.
ACCESSION   DATDBZ010000507 DATDBZ010000000
VERSION     DATDBZ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2624969"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="VIX62_11855"
     CDS             <1..1280
                     /locus_tag="VIX62_11855"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5860971.1"
                     /translation="FADYLAVVERTGVGINIAPLVGHGTVRLAVLGYEQRPPTAGELG
                     AMRSHVDEAMRAGAFGLSTGLVYPPGCFGDTDEVVALAEIVAGYDGLYASHIRGERET
                     VVEAVRECLEIGERARCRVQVSHNAPKWGGSHLLPDVMALWEAARERGLDVGVDNDAH
                     TDFAWGLRNGLPQWTHELGPDDLLALIGTKRDALIDEVERDERPAYGPVGLLKHGAWD
                     RIWVLPAGDAGSLAGRTIASIAQERRVDGWSAYLAVIVETRGSAWGLFDYIEESVVRA
                     VAAHPMVMYCSDGWVLPRGVERGRRPVYMPCSYGEFPGMLERWVVGTPDSPPLVDLET
                     AIRKMTWMPASRLGLRDRGRIIEGAMADLVVLDLPNVHDRATNRFPHDPIDANYPHAS
                     PSGIDWVLVNGVVAVDHGSTTGVLAGRVLRHGA"
     gene            complement(1295..2212)
                     /locus_tag="VIX62_11860"
     CDS             complement(1295..2212)
                     /locus_tag="VIX62_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860972.1"
                     /translation="MAIEQSREMRSPRSLVWQRRRATAAEHWRTFRRNRQGMVGLGIL
                     SFFVIVAVVGPMFIDPAAIEPATADGPLLAPPSIDYPLGTDNFGRSVLLLMVQGARIS
                     LLVGFTAAFGTMLIGSSIGITAGYFGGTRVDTMLNAVTNWFLVLPWIALAIALTAVIG
                     PTLVNVIIVIAITSWASTARLVRSQALSVKERPFMERSRALGAGHWHMITRHVLPNVF
                     PILFANAVLMIALAILSETTLSILGLGPANAISWGRIIDDSFSAGALSAGWWWWLIPP
                     GVAIMFVTLSFTMVGFALDEVLSPRLRSR"
     gene            complement(2214..3179)
                     /locus_tag="VIX62_11865"
     CDS             complement(2214..3179)
                     /locus_tag="VIX62_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5860973.1"
                     /translation="MGARRYVGRKFLQALLTLAFVLTFNFVLFRVLPGDPVGLLARSQ
                     RLTEQDVAALQAELGLDQPIVRQFVTYAGQTLTGNLGVSLRSAREVTTTIWSRIGPTV
                     LLVGLGTLFATVIGIWIGIKGGWRRGSTFDTTSLYGSLALYSIPEGWLGMLLLLVLSG
                     MLGLFPAGGFETGGETGVARLVDVLDHLFLPVLTLTLGYVGQYAIIMRSSMIDVTHED
                     YVTSARAKGVPEHLVRRNHVVPNAFLPTFTLIFLNFGFVLGGAIVVEAVFSWPGLGLL
                     TYDAINQLDYPVIQGVFLLSSAAVIAFNLVADITYGYLDPRVREA"
     gene            complement(3204..>3917)
                     /locus_tag="VIX62_11870"
     CDS             complement(3204..>3917)
                     /locus_tag="VIX62_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860974.1"
                     /translation="LLPGYNYDSLVSLRAVDASVAATSGGSSGIPGWIWLVAGVAVVA
                     LIVGFLDQIGIQVELRPVSDGKMNDYWGAGNFDAYIWYWSGDPDPNYQLFVFTSEQCG
                     AWSDGCWQDPTFDSLYEEQRETFDREARQEIVFEAQRRVYEQAPGVVLAYPGSLQAYR
                     NDRFTGWVPNPGDDGYLLPGYNYDSLVSLRAVDDSVAATSGGSSGIPGWLWLVAGAAV
                     VAVIVVMSRRGRRRDLDEA"
BASE COUNT          676 a         1359 c         1337 g          545 t
ORIGIN      
        1 ccttcgccga ctacctcgcc gtcgtcgaac gcaccggcgt cggcatcaac atcgcgccgc
       61 tcgtggggca cggcaccgtg cgcctagcag tgctcgggta cgagcagcgc ccaccgaccg
      121 ccggcgagct gggcgcgatg cgctcccacg tcgacgaggc gatgcgagcc ggtgccttcg
      181 gcctgtcgac cgggctcgtc tatccacccg gatgcttcgg cgacaccgac gaggtggtgg
      241 ccctcgcgga aatcgttgcc ggatacgacg ggctgtatgc gagccacatc cggggcgagc
      301 gagagacggt cgtcgaagcc gtgcgcgaat gcctcgagat cggcgagcgg gccaggtgca
      361 gggtgcaggt cagccacaac gcaccgaagt ggggaggctc ccacctgctg cccgacgtga
      421 tggcgctctg ggaagcggcg cgcgagcgcg gcctcgacgt cggcgtcgac aacgacgcgc
      481 acaccgactt cgcctggggg ctgcgcaacg gactgccgca atggacccac gaactcgggc
      541 ccgacgatct gctggcgctc atcggcacga agcgtgacgc gctcatcgat gaggtcgagc
      601 gcgacgaacg tccggcctac gggccggtcg gactgctgaa gcacggggcg tgggatcgca
      661 tctgggtgct accggccggc gacgccggtt cgctcgccgg tcgcacgatc gcctcgatcg
      721 cccaggagcg ccgcgtcgac gggtggtcgg cctacctcgc ggtcatcgtc gagacccggg
      781 gctcggcctg ggggttgttc gactacatcg aggaatcggt ggtgcgcgcg gtcgcggccc
      841 atcccatggt catgtactgc tccgacgggt gggtgctgcc gagaggcgtg gagcgtggtc
      901 gccggcccgt gtacatgccc tgcagctacg gggagttccc cggcatgctc gagcgctggg
      961 tcgtcggtac ccccgactcc ccgccgctcg tcgatctcga gacggccatc cggaagatga
     1021 cctggatgcc ggcgtcccgg ctcgggttgc gcgatcgtgg ccgcatcatc gaaggggcga
     1081 tggccgatct cgtcgtgctc gacctgccca acgtgcacga ccgggcgacg aaccgcttcc
     1141 cgcacgatcc gatcgacgcc aactacccgc atgcgagccc cagcggcatc gactgggtac
     1201 tggtgaacgg cgtggtcgcc gtcgatcacg gctcgaccac cggcgtgctg gccggtcgcg
     1261 tgctgcggca cggggcctga cggctccgac gggactagcg cgagcggagg cgggggctta
     1321 gcacctcgtc gagcgcgaac cccaccatcg tgaacgacaa cgtcacgaac atgatggcca
     1381 cccctggcgg gatcagccac caccaccacc ccgccgacaa cgccccggcc gagaacgagt
     1441 cgtcgatgat gcgaccccat gagatcgcgt tcgccggccc gaggccgagg atcgacagcg
     1501 tcgtctcgga caggatcgcg agggcgatca tcaacacggc gttcgcgaac aggatcggga
     1561 acacgttggg cagcacatgg cgggtgatca tgtgccagtg accggcgccg agcgctcggc
     1621 tgcgctccat gaacggccgt tccttgaccg agagggcttg cgaccgcacg agtcgcgccg
     1681 tgctcgccca cgaggtgatc gcgatcacga tgatcacgtt caccagggtg ggtccgatca
     1741 ccgccgtcag ggcgatcgcg agcgcgatcc agggcagcac caggaaccag ttcgtcacgg
     1801 cgttcagcat cgtgtcgacc cgcgtgccgc cgaagtaccc ggcggtgatg ccgatgctcg
     1861 aaccgatgag catcgtcccg aacgccgccg tgaaccccac gagcagcgag atgcgcgcgc
     1921 cctgcaccat gagcagcagc accgatcgtc cgaagttgtc cgtgccgagc gggtagtcga
     1981 tcgacggagg agcgagcagg ggtccgtcgg ccgtggccgg ctcgatggcc gcggggtcga
     2041 tgaacatcgg tcccacgacc gccacgatga cgaagaacga gaggatgccg agccctacca
     2101 tgccctggcg gttgcgccgg aacgtgcgcc agtgctcggc tgcggtggcc cgccgccgct
     2161 gccacaccaa cgagcggggc gagcgcatct cccgcgactg ctcgatcgcc acgtcaggcc
     2221 tcccgcacgc gggggtcgag gtagccgtac gtgatgtcgg cgaccaggtt gaacgcgatc
     2281 accgcggcgc tcgagagcag gaagaccccc tggatcaccg ggtagtcgag ctggttgatc
     2341 gcgtcgtagg tgagcagccc gagccccggc cacgagaaga ccgcctcgac cacgatcgcc
     2401 ccgccgagca cgaacccgaa gttcaggaag atcagcgtga acgtcggcag gaatgcgttc
     2461 ggcaccacgt ggttgcgccg cacgaggtgc tcgggcaccc ctttggcgcg cgcgctcgtg
     2521 acgtagtcct cgtgggtcac gtcgatcatc gacgagcgca tgatgatcgc gtactggccc
     2581 acgtagccga gggtgagggt cagcaccggc aggaagagat ggtcgagcac atcgaccaac
     2641 cgcgccacgc cggtctcgcc gccggtctcg aagccgccgg ccgggaagag cccgagcatg
     2701 ccggagagca ccagcagcaa cagcatgccg agccagccct ccgggatcga gtagagcgcg
     2761 agcgacccgt agagcgatgt cgtgtcgaac gtgctgccgc ggcgccatcc gcctttgatg
     2821 ccgatccaga tgccgatcac ggtggcgaag agcgtgccga gcccgacgag cagcacggtc
     2881 ggcccgatgc gcgaccagat cgtggtcgtg acctcccggg cgctgcgcaa cgacacgccg
     2941 aggttgccgg tcaaggtctg gccggcgtac gtcacgaact gccgcacgat cggctgatcg
     3001 agtcccagct cggcctgcag ggcggcgacg tcctgctccg tgagccgctg gctccgcgcc
     3061 agcaggccga ccggatcacc gggcagcaca cggaacaaca cgaagttgaa ggtcagcacg
     3121 aacgcgagcg tgagcagggc ctgcaggaac ttccgaccca cgtagcgccg cgcgcccacc
     3181 ccgccgcccg ctcgccgttg cgctcaggcc tcgtcgagat cgcgccgtcg tccgcggcgc
     3241 gacatcacga cgatcaccgc gaccaccgca gcgccggcca ccaaccagag ccaccccggg
     3301 atgccgctcg agcctcccga cgtggcggcc accgagtcgt cgaccgcacg cagcgacacg
     3361 agcgagtcgt agttgtaacc cggcagcagg tagccgtcgt cacccgggtt cggcacccac
     3421 ccggtgaagc ggtcgttgcg gtaagcctgc agcgacccgg gataggccag cacgacgccg
     3481 ggggcctgtt cgtacacccg gcgctgtgcc tcgaacacga tctcttgccg ggcttcccgg
     3541 tcgaacgtct cgcgctgttc ctcgtagagc gaatcgaacg tcgggtcctg ccagcatccg
     3601 tcgctccacg cgccacattg ctcggacgtg aacacgaaga gctggtagtt cggatcgggg
     3661 tcgccgctcc agtaccagat gtaggcatcg aagttgccgg caccccagta gtcgttcatc
     3721 ttcccgtcgg agaccgggcg gagttccacc tggatgccga tctggtcgag gaatccgacg
     3781 atgagcgcga ccacggccac gccggccacg agccagatcc accccgggat gccgctcgag
     3841 ccgcccgacg tcgcggccac cgacgcatcg accgcacgca gcgagaccag ggagtcgtag
     3901 ttgtagcccg gcagcag
//
LOCUS       DATDBZ010000508         4320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_795818, whole genome shotgun sequence.
ACCESSION   DATDBZ010000508 DATDBZ010000000
VERSION     DATDBZ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795818"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /gene="dnaE"
                     /locus_tag="VIX62_11875"
     CDS             complement(<1..1687)
                     /gene="dnaE"
                     /locus_tag="VIX62_11875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY5860975.1"
                     /translation="MSERSTDGFVHLHVHSEYSLLDGAARIQSPKSNPGAPTIFTEAQ
                     RLGMPAVAITDHGSMFGALPFFEAGRASGVKPILGVEAYVAPGSRFDRNPGESEEKYY
                     HLTLLAENETGYRNLLKLVSYAHLEGFYHRPRMDKQLMAEYAEGVICLSGCLGSELAQ
                     QLLNGQDDRARQTAAEYRDIFGPDNYFIELQDHGIGEQQKILRRQIDLGHELDIPIVG
                     TNDLHYTLKEDAKPHDVLLCIQQQKVQTDMKRLRFETEEFYLKSAEEMREVFRELPEA
                     CDNTLAIAERVELDLVYGDRAPADQRYHLPRFETPGGLDRDTYLRQLVDDGAAQRYPE
                     ITTEVRDRIDHELGVITSMGFGGYFLIVWDLIRYAREHGIRVGPGRGSAAGSVVSYCL
                     RITDLDPLRYGLLFERFLNPERIQMPDIDMDFDERRRDEVIRYATEKYGSDHVAQIVT
                     FQTIKGKQGIRDAARVLGFPPVVGDRLCKMYPPAVMGRDYPIEDALKLSPDLAAAYEK
                     EPEAKEIIETARALEGLRREDSVHAAGVVIGDAPLVNYLPLKLSKDSRDDSKKV"
     gene            complement(1846..2754)
                     /locus_tag="VIX62_11880"
     CDS             complement(1846..2754)
                     /locus_tag="VIX62_11880"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY5860976.1"
                     /translation="MTREAHVATPGRLDAVLAELTGLPRADIQRAITAGRVTVDGEPR
                     AKSFRLAGGEALVIEVPDDAELEPEGPAVPVRYRDEALLVIAKPPGLVTHPTENRRSG
                     TLVNRLLGMGEPLSTTGGPLRPGVVHRLDAGTSGLMVIARTDRAHEALAEMLKDHAIE
                     RRYLALVRGEVEHEAFAIDAPLGRRADKVVVDHTEGRPAETAFEVRERFDGLTLLEAI
                     PKTGRTHQIRVHLQAIGHPIVGDKAYGGGGDAAKRLGLQRPFLHSWKIAFDQPITEER
                     IELEEPLPDDLETALRLARTQTIERA"
     gene            complement(2744..3235)
                     /gene="lspA"
                     /locus_tag="VIX62_11885"
     CDS             complement(2744..3235)
                     /gene="lspA"
                     /locus_tag="VIX62_11885"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY5860977.1"
                     /translation="MDRVQRRGATLLFAFAGFAYLLDRLTKLWAERTLQDAPADLIPG
                     VLTLRFTTNSGGAFSIGGSAPWLFAGATIIVSVLIVATAFRHRNMLTAVALGLILGGA
                     LGNLTDRAVRGSGLHGRVIDFIDFHIWPIFNLADSAIVVGAVLLAIASMRPDEVSEAD
                     VDA"
     gene            complement(3240..3623)
                     /locus_tag="VIX62_11890"
     CDS             complement(3240..3623)
                     /locus_tag="VIX62_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY5860978.1"
                     /translation="MAETKLTKKEITDLQARLVEERGELQTQLATLEEDTFATTQSEM
                     SGDVGLDDESADAGTATFEREKDLSIEQNVRDLLDKIERALKRIDDGTYGVCERCGKS
                     IEKARIKALPYVDLCIKDAQAQSRR"
     gene            4076..>4320
                     /locus_tag="VIX62_11895"
     CDS             4076..>4320
                     /locus_tag="VIX62_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005478205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5860979.1"
                     /translation="MAKRFESVYAKVSFPELERGILGFWKDRDVFARSLAQREGAPAW
                     IFYEGPPTANGKPGVHHVESRTFKDIYPRYKTMTGYRV"
BASE COUNT          640 a         1437 c         1537 g          706 t
ORIGIN      
        1 cgaccttctt cgagtcgtcg cgcgagtcct tcgacagctt cagcggtagg tagttcacga
       61 gcggggcatc gccgatcacc acgccggcgg cgtgcacgga gtcctcgcgg cgcagaccct
      121 cgagcgcacg tgcggtctcg atgatctcct tggcctcggg ttccttctcg taggccgcgg
      181 ccagatcggg cgagagcttc agcgcgtctt cgatcgggta gtcccggccc atcaccgccg
      241 gcggatacat cttgcagagg cggtcgccca ccaccggcgg gaagcccagc acgcgcgcgg
      301 cgtcgcggat gccctgcttg cccttgatcg tctggaaggt gacgatctgg gcgacgtgat
      361 ccgagccgta cttctcggtg gcgtagcgga tcacctcgtc gcgacgacgc tcgtcgaagt
      421 ccatgtcgat gtcgggcatc tggatgcgct cgggattgag gaagcgctcg aagagcaggc
      481 cgtagcgcaa cggatcgagg tcggtgatgc gcaggcagta cgacaccacc gagccggccg
      541 cgctgccgcg gccgggcccg acgcggatgc cgtgctcgcg cgcgtagcgg atcaggtccc
      601 acacgatcag gaagtagcca ccgaacccca tcgacgtgat gaccccgagc tcgtggtcga
      661 tgcggtcgcg tacctccgtg gtgatctcgg ggtagcgctg cgccgcaccg tcgtcgacca
      721 gctgacgcag ataggtgtcg cggtcgaggc cgccgggggt ctcgaacctg ggcaggtggt
      781 agcgctggtc ggcgggcgcg cgatcgccgt agacgaggtc gagctcgacg cgttcggcga
      841 tcgccagggt gttgtcgcac gcctcgggga gctcacggaa gacctcgcgc atctcctcgg
      901 cgctcttgag gtagaactcc tcggtctcga accggaggcg cttcatgtcg gtctgcacct
      961 tctgctgctg gatgcacagc aggacgtcgt gcggcttggc gtcctccttc aacgtgtagt
     1021 gcaggtcgtt cgtgccgacg atggggatgt ccagctcgtg gcccaggtcg atctggcggc
     1081 gcaggatctt ctgctgctca ccgatcccgt ggtcctggag ctcgatgaag tagttgtcgg
     1141 gcccgaagat gtcgcggtac tcggcggcgg tctggcgcgc gcggtcgtcc tggccgttca
     1201 gcagttgctg cgcgagctct gaacccaggc agcccgacag gcagatgacg ccctcggcgt
     1261 attcggccat gagctgcttg tccatgcgcg gccggtggta gaagccctcg aggtgggcgt
     1321 agctcaccag cttcaacagg tttcggtacc cggtctcgtt ctcggccagc agggtgaggt
     1381 ggtagtactt ctcctcgctc tcgccggggt tgcgatcgaa ccgtgagccc ggagccacat
     1441 aggcctccac acccaggatc ggcttgacgc ccgaggcccg cccggcctcg aagaacggca
     1501 acgcgccgaa catcgatccg tggtcggtga tggccaccgc cggcatgccc aggcgctggg
     1561 cctcggtgaa gatcgtgggg gcgccggggt tggacttcgg cgactggatg cgcgcggctc
     1621 cgtcgagcaa cgagtactcc gagtgcacgt gcaggtggac gaagccgtcg gtggatcgtt
     1681 cgctcatcgc cgctgctcag gccccccgcg aggcgaaccc actcgggtta tccacactgg
     1741 ggataactta catccgtgtg attcgccgga ggttgaacgt acgcccggct cggaccatcg
     1801 tcaagggtgc aggtcgggag cctcggccgg gagcctcggg gaacctcacg ctcgctcgat
     1861 cgtctgcgtc cgggcgagcc gcagcgcggt ctcgagatcg tcggggaggg gttcctcgag
     1921 ctcgatccgc tcctccgtga tgggctggtc gaacgcgatc ttccacgagt gcaggaatgg
     1981 gcgctgcagg ccgagccgct tcgcggcatc gcccccgccc ccgtaggcct tgtcccccac
     2041 gatcgggtgc ccgatcgcct gcagatggac gcggatctgg tgggtacggc cggtcttcgg
     2101 gatcgcctcg aggagcgtga ggccgtcgaa gcgctcacgg acctcgaagg ccgtttccgc
     2161 cggccggccc tcggtgtgat cgacgaccac cttgtccgcc cgccgaccca gcggtgcgtc
     2221 gatggcgaac gcctcgtgct ccacctcgcc gcgcaccagc gccaggtatc ggcgttcgat
     2281 cgcatgatcc ttcagcatct cggcgagtgc ctcgtgggca cggtcggtgc gcgcgatcac
     2341 catgagcccc gaggtgccgg catcgagccg gtgcacgacg ccggggcgca gcggaccccc
     2401 cgtcgtcgag agcggctcac ccatgcccag cagccggttc accaacgtgc cgctgcgccg
     2461 gttctcggtc gggtgcgtga ccaggccggg cggcttggcg atcaccagca gggcctcgtc
     2521 gcggtagcgg acggggaccg ccgggccctc gggctcgagt tcggcgtcgt ccgggacctc
     2581 gatcacgagc gcctctcccc cggccaggcg gaacgatttc gcgcggggtt cgccgtcgac
     2641 cgtgacgcgc ccggcggtga tcgctcgttg gatgtcggcg cgaggcagcc cggtcagctc
     2701 cgccagcacg gcgtcgaggc gtccgggggt cgcgacgtgc gcctcacgcg tcaacgtcgg
     2761 cctcgctgac ctcgtccggg cgcatcgagg cgatcgccaa cagcaccgcg cccacgacga
     2821 tcgcggaatc ggcgaggttg aagatgggcc agatgtggaa gtcgatgaag tcgatgacgc
     2881 ggccgtgcaa ccccgatcca cgcaccgccc ggtcggtgag gttgccgagc gcgccgccca
     2941 ggatcaggcc gagcgcgacc gcggtcagca tgttccggtg gcggaacgcg gtggcgacga
     3001 tgagcaccga gacgatgatc gtggcgccgg cgaacaacca cggggcgctg ccgccgatgc
     3061 tgaacgcacc gcccgagttc gtcgtgaacc gcagcgtcag caccccgggg atcaggtcgg
     3121 cgggcgcgtc ctgcagggtt cgctccgccc agagcttcgt gaggcggtcc agcaggtagg
     3181 cgaacccggc gaaggcgaac aggagggtgg caccgcgccg ctgtacgcgg tccacggcgc
     3241 tatcggcgcg actgcgcctg agcgtccttg atgcagaggt cgacgtacgg cagcgccttg
     3301 atgcgagcct tctcgatgga cttgccacac cgctcgcaga ccccgtaggt gccgtcgtcg
     3361 atgcgcttga gcgcacgttc gatcttgtcg aggaggtcgc ggacgttctg ctcgatcgac
     3421 aggtccttct cgcgttcgaa ggtggccgtt ccggcgtcgg cggactcgtc gtccaggccg
     3481 acgtcaccgg acatctcgga ctgggtggtg gcgaacgtgt cctcttcgag ggtggcgagc
     3541 tgggtctgca actcgccccg ctcctcgacg aggcgggcct ggagatcggt gatctccttc
     3601 ttcgtcagct tggtctcggc catggtcggc gaagggtagc agatggggct ctcagaagcg
     3661 catcacccca ggtcagaggg cctgttccgc ggtccacgcc ctggcaaccg ccaccccctc
     3721 ccccgggccc cggatcggcc ggtgtagcat gccggtcaca ggcagtgacg ggaacgcggt
     3781 cgtggggacc gtggcgccgc agagcgaacc ggggacggtg ggagcccggt ggccagcacc
     3841 ccgcgatcat cctccccgag cccctgcccg aaacgggcgc cgacggccgg cgcaccggag
     3901 tagacgcagg cgggttccgc ccgtcatcgc ggaggaccgt gcaggcggtc ccagagcggt
     3961 cgccggtcgc gagaccggtg gcaagcgggg tggtaccgcg gggcccacgc ctcgtcccgg
     4021 cgtcggatgg gacggggcgt tttccatgcc cggacgcggt tgaggaaggg acgagatggc
     4081 gaagcggttc gagtcggtgt acgccaaggt gagcttcccg gagctcgagc gcggcatcct
     4141 ggggttctgg aaggaccggg acgtgttcgc gcgctcgctc gcccaacgcg agggcgcccc
     4201 cgcctggatc ttctacgagg gaccacccac ggccaacggc aagccgggcg tccaccacgt
     4261 ggagtcgcga accttcaagg acatctaccc gcggtacaag acgatgaccg ggtatcgggt
//
LOCUS       DATDBZ010000509         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2829192, whole genome shotgun sequence.
ACCESSION   DATDBZ010000509 DATDBZ010000000
VERSION     DATDBZ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829192"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VIX62_11900"
     CDS             <1..1425
                     /locus_tag="VIX62_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEY5860980.1"
                     /translation="LAESGRRVLVLEAGPDYGSFGGLGWPSDLLDASTIPISHDWGFR
                     SDSELAGRDLAFERARVIGGCSAHNGCTASWGHRADYDGWVARGLPGWGADALLSSFQ
                     EASVRMRVRRFRDDEIVPFHRAFLDAGAQMGLQELDDLESLDGVPSVCAQPSNSPDGV
                     RWNAAFAYLDPVRDRPDLEIRGLATVERVVFEGNRAAGVRWRDEGGTQEAWADLIVLA
                     GGTYGSPELLLHSGVGPAQDLWDLGIPIVADVAGVGRNLHDHPSFELAFEPTEELASR
                     TKAFAATGKPVPDEQGFAKVASSTASNGVFDLHVFPETAMDGRTAIFAAYLTPRSRGS
                     VTLRSSTPGEPPRIDHAYLSDEEAHDLAALTEGVEVARAFASSDAMRPLLGRELDPGS
                     GARTLNTLREAIRKGVIHYWHPVGTCAMGSDDDPDAVTDADGKVRGVEGLVVADASLM
                     PATVRATTNLPTVVIGERIAAALV"
     gene            1531..>2240
                     /locus_tag="VIX62_11905"
     CDS             1531..>2240
                     /locus_tag="VIX62_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEY5860981.1"
                     /translation="MEFEIGIGKTARRAYGFDEVAIVPSRRTRDPQDVDIRWEIDAYT
                     FELPMMASAMDAAVSPATAVEIGRVGGLACLNLEGLWTRFEDPEPIYAEIAELSDEKA
                     TRRMQELYAEPVQEDLIGRRIEEIKAGGVISCASLTPQRVEQYAKLVLDAGLDILVIQ
                     GTVVSGEHVSTRQEPLNLKEFIPTFDIPVIVGGCASYSTALHLLRTGAVGVLVGVGPG
                     NACTTRGVLGVGVPQATA"
BASE COUNT          347 a          736 c          782 g          375 t
ORIGIN      
        1 ctcgccgagt cgggtcgtcg agtgctggtc ctcgaagccg ggccggacta cggctccttc
       61 ggaggcctcg gatggccaag cgacctgctc gatgcctcga cgatcccgat cagtcacgat
      121 tggggtttcc gatcggactc cgagctcgcc gggcgcgacc tcgcgttcga acgtgcgcgt
      181 gtcatcggcg gttgctccgc tcacaacggg tgcaccgcgt catggggtca tcgggccgac
      241 tacgacggtt gggtggcgcg agggctcccg gggtggggcg cggacgccct cctgtcgtcc
      301 ttccaggagg cgtcggtgcg catgcgggtg cgccgattcc gcgacgacga gatcgtgccg
      361 ttccaccgcg cgttcctgga cgccggcgcc cagatgggac tgcaggagct cgacgacctg
      421 gagtccctcg acggggtgcc ttcggtgtgt gcgcagccgt ccaacagccc ggatggcgtg
      481 cgatggaacg ccgccttcgc ctacctcgat ccggtgcggg atcgtcccga cctagagatc
      541 cgtggactcg cgacggtcga gcgcgtcgtg ttcgagggga accgcgcggc aggcgtgcgt
      601 tggcgggacg aaggcggcac ccaggaggct tgggccgatc tgatcgtgct cgccggcgga
      661 acgtacggca gtcctgagct cttgctgcac agtggcgtgg gtcctgccca agatctctgg
      721 gacctcggca tccccatcgt cgccgacgtg gcgggcgtcg gccgcaacct gcatgaccat
      781 cccagtttcg agctcgcgtt cgaaccgacg gaggagctgg cgtccagaac gaaggccttc
      841 gccgcaaccg gcaagccggt gcccgacgag caagggttcg cgaaggtggc atcctcgacc
      901 gcgtcgaacg gcgttttcga cctgcatgtc ttccccgaga ccgccatgga cggtcggacc
      961 gcgatcttcg cggcctacct cacgccgcgc tctcgcgggt cggtgacgct ccgcagctcg
     1021 acgcctggcg agccgccacg gatcgaccac gcctacctca gtgatgagga ggcacatgac
     1081 ctcgccgcgc tcaccgaagg cgtcgaggtc gcgagggcct tcgcgtcgag cgacgcgatg
     1141 cgaccgctcc tcggacgcga actcgacccg ggatccggcg ctcgcacgct caacacgctt
     1201 cgtgaggcga tccggaaggg ggtgatccac tattggcatc cggtggggac gtgcgcgatg
     1261 ggttcggacg acgatcccga cgcggtgacc gacgccgatg gcaaggtccg aggggtggaa
     1321 ggtctggtgg tcgccgacgc ctcgttgatg cctgccaccg tccgtgccac caccaacctg
     1381 ccgacggtcg tgatcggtga acggatcgcg gcggcgttgg tctagccccc cgaacccgtc
     1441 tgttaccctt cgttcatgta gggatcgacg cccatccggc gaccgggtgg gcgttctgcg
     1501 tttctggagc acgactgggc aggggtggag atggaattcg agatcggcat cggcaagacc
     1561 gctcgaaggg cctacggctt cgatgaagtc gcgatcgtgc cttcacgtcg aacgcgggac
     1621 ccgcaggacg tcgacatccg ttgggagatc gacgcgtaca ccttcgaact gccgatgatg
     1681 gccagtgcca tggacgcggc ggtctcgccc gccacggccg tggagatcgg ccgcgtcggc
     1741 ggcctcgcgt gcctgaacct cgagggactg tggacgcgtt tcgaggaccc cgagccgatc
     1801 tacgcggaga tcgccgagtt gtcggatgag aaggctaccc gccgcatgca ggagctctac
     1861 gccgagccgg tgcaagaaga tctgatcgga cggcgcatcg aagagatcaa ggccggtggc
     1921 gtcatcagct gcgcctcgct cacgccccaa cgcgtcgagc agtacgcgaa gttggtcctg
     1981 gacgccgggc tcgacatcct ggtgatccaa ggcacggtcg tgtcgggaga acacgtttcg
     2041 acccgccagg agccgctcaa cctgaaggaa ttcatcccga cgttcgacat cccggtgatc
     2101 gtcggcggat gcgcctccta ctccacggcg ctgcacctgc tgcgcaccgg ggctgtcggt
     2161 gtgctcgtgg gcgttgggcc cggcaacgcg tgcaccacgc gaggtgtgct cggcgtcggc
     2221 gtgccgcagg cgacggccat
//
LOCUS       DATDBZ010000510         2588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1181709, whole genome shotgun sequence.
ACCESSION   DATDBZ010000510 DATDBZ010000000
VERSION     DATDBZ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181709"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VIX62_11910"
     CDS             <1..1067
                     /locus_tag="VIX62_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEY5860982.1"
                     /translation="PAAATWGGVAGTRTGVKCLHAHYAYHLAGGVDPIGAWVAERVEP
                     VHADAPIERVAAIDQGTNSTRLLVADGAGTELARDMVITRLGQDVDRTGRLDPEALGR
                     TVDVLAGFCRRARALGAGRIRVVATSAVRDAQNRVEFETAVLEHAGTALEVITGEQEA
                     ALSFLGATHGLEPADGPFLVQDIGGGSTEFVTGAEPGLAEHAISTQMGSVRLTERHIA
                     HDPPTPGELAALFETVNEVLTQVERDVPSAPMRTFVAVAGTATTVQAINLGLGRYDPD
                     RIHRSWLTVDDAARVLADLSAMTDAQRAAIPVMVPGRGDVIIAGAVILVATMRRFGVA
                     RAMVSETDILDGLALQMLDG"
     gene            1080..1904
                     /locus_tag="VIX62_11915"
     CDS             1080..1904
                     /locus_tag="VIX62_11915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5860983.1"
                     /translation="MPKRTRDKQLAKLAARRQAERRAIRRRRNLWFGLVAGAAALVLL
                     IVAASVILAGDDPASTPAAGVSPSVSASPAGDIPKSCSSEAPPGADETKPAFDEPAPD
                     TIDPSKAYAATIDTSCGVIQVDLLADTATEAVNSFVFLAQEGFYDGLTWHRLVEGFVI
                     QGGDPEGVGTGGPGYETPVTVTEGSSFDAKGIVAFAHAATGGNGSQFFITLGPTPNLD
                     PPQGEYTIFGNVTKGMDVVDTIASFPVVNSSGCPEGQPPCAPAQPVYINSITIEES"
     gene            complement(1901..>2588)
                     /locus_tag="VIX62_11920"
     CDS             complement(1901..>2588)
                     /locus_tag="VIX62_11920"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HEY5860984.1"
                     /translation="AVLDGLAAGARDFGTVVRLTPDIVRDLGTEQAQRVLDVALRFAG
                     HGVVALNCAGSERAGIEPFGPYFRTAKEAGLGSVPHAGEWAGPQNVWDTLATFEPDRI
                     GHGVRAIDDPELVATLADRAIPLEISPLSNVATGVYPSLAEHPFMRLRDAGVIVTLNS
                     DDPPMFGDAWVGRVYEAARDAWGLDDGHLARIARTAVDVSFADAATKREIGAGIDAWL
                     GSEPEPVTAP"
BASE COUNT          407 a          903 c          883 g          395 t
ORIGIN      
        1 tgcccgcggc ggccacgtgg ggtggcgtgg ccgggacccg cacgggtgtg aagtgcctgc
       61 acgcccacta cgcctaccac ctcgccggtg gcgtcgaccc gatcggcgcg tgggtcgcag
      121 aacgcgtcga gcctgtccac gccgacgcgc ccatcgagcg tgtcgccgcg atcgaccaag
      181 gcacgaactc gacgcggctc ctcgtcgcgg acggggccgg caccgaactc gctcgtgaca
      241 tggtcatcac gcggctcggc caggacgtcg atcgaacggg caggctcgac cccgaggcgt
      301 tggggcgaac ggtcgacgtg ctcgcgggct tctgccgccg cgcccgcgcc ctgggtgccg
      361 gtcgcatcag ggtggtggcg acaagtgcgg tccgtgacgc ccagaaccgt gtggagtttg
      421 aaaccgccgt cctcgagcac gctggaaccg cgctggaggt catcacgggc gagcaggaag
      481 ccgcgctgtc gttcctgggc gcgacgcatg ggctcgaacc cgccgacgga ccgttcctgg
      541 tgcaggacat cggcggcgga tcgaccgagt tcgtcacggg cgccgaaccc ggtctcgctg
      601 agcacgcgat ctccacccag atgggcagtg tgcggctcac cgaacgtcac atcgcccacg
      661 atccgccgac gccaggtgaa ctggccgcgc tcttcgagac ggtgaacgag gtcctcacgc
      721 aggtcgagcg ggacgtccca tcggcgccga tgcgaacgtt cgtggcggtg gctggtaccg
      781 cgacgaccgt gcaggccatc aacctcgggc tcggtcgcta cgaccccgac cggatccacc
      841 gatcctggct cacggtggat gacgctgcgc gagtgctcgc cgatctctcg gctatgaccg
      901 acgcccaacg tgcggcgatc cccgtcatgg tgccgggccg cggtgacgtc atcatcgcag
      961 gcgcggtgat cctggtggcg acgatgcgcc ggttcggcgt ggcccgggcg atggtcagcg
     1021 agaccgacat cttggatggt ttggctctgc agatgctcga cgggtaacct catcgcccga
     1081 tgcccaaacg aaccagagac aagcaactcg cgaagttggc ggcgcgccgg caggccgaac
     1141 gccgggccat caggcgccgc cggaacctgt ggttcggtct ggtggcagga gccgcagcgc
     1201 tggtcctgct catcgtcgcg gcgagcgtca tcttggccgg cgacgatccg gcgtccacac
     1261 cggccgccgg tgtttcgccc agcgtcagtg cgtcgcccgc cggtgacatc ccgaagtcgt
     1321 gttccagcga agcccctccg ggtgccgacg agaccaagcc cgccttcgac gagccggcgc
     1381 cggacacgat cgatccgtcg aaggcatacg ccgccacgat cgacacgtcc tgtggcgtga
     1441 tccaggtcga cctcttggcg gatacggcga ccgaagccgt caacagcttc gtgttcctgg
     1501 cccaggaagg gttctacgac gggctcacct ggcaccggtt ggtcgagggg ttcgtgatcc
     1561 agggtggtga ccccgagggg gtggggaccg gcggtcctgg ctacgagacc cccgtcacgg
     1621 tgaccgaagg ctccagcttc gatgccaaag gtatcgtcgc gttcgcacat gccgccaccg
     1681 ggggcaacgg gagccagttc ttcatcacgt tgggtcccac gcccaacctc gaccctccgc
     1741 agggtgagta cacgatcttc ggcaacgtca cgaaaggcat ggacgtggtc gacaccatcg
     1801 cctcgttccc cgtggtcaac agctcggggt gccccgaagg ccagcctccg tgcgcacctg
     1861 cacagccggt ctacatcaac tcgatcacga tcgaggagtc ctagggagcg gtcaccggtt
     1921 cgggttcgct cccgagccag gcgtcgatgc ccgcgccgat ctcgcgcttg gtcgccgcgt
     1981 cggcgaacga gacatcgacg gcggtgcgcg cgatccgtgc gaggtgcccg tcgtccagtc
     2041 cccacgcatc gcgagccgct tcgtacaccc ggccgaccca tgcatcaccg aacatcggtg
     2101 ggtcgtcgga gttgagggtc acgatcacac cagcgtcgcg cagtcgcatg aacgggtgct
     2161 cggccagcga cgggtacacg ccggtcgcca cgttcgacag cggtgagatc tcgagcggga
     2221 tcgcacggtc ggccagcgtc gccaccagtt ccgggtcatc gatcgcgcgg actccgtgcc
     2281 cgatccggtc gggctcgaac gtcgccagtg tgtcccagac gttctggggc ccggcccatt
     2341 cgcccgcgtg cggcaccgag cccaagccgg cctccttcgc cgtgcggaag tacggcccga
     2401 acggttcgat gccggcacgt tcgctcccgg cgcagttgag cgcaacgacc ccatggccgg
     2461 cgaaccggag ggcgacgtcg agcacgcgtt gggcctgctc ggtgccgagg tcccgcacga
     2521 tatcgggcgt gaggcgaacg acggtcccga agtcccgcgc tcccgccgcg aggccatcga
     2581 gcactgcc
//
LOCUS       DATDBZ010000511         2206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1997487, whole genome shotgun sequence.
ACCESSION   DATDBZ010000511 DATDBZ010000000
VERSION     DATDBZ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997487"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VIX62_11925"
     CDS             complement(<1..976)
                     /locus_tag="VIX62_11925"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY5860985.1"
                     /translation="MKRPIRIAALVALFGLTLFACYVRLPYYAVGPGPAREVGPLIDV
                     KGHQRYDSAGKLIMTTVSYAQVTALQALGAWFDPVTQVVHRDVLFAPGEDPSTERQRA
                     ISEMDTSKINATYVVMQHLTDYPKEHADGALIQATVEGCPAFDELFPGDLITAIGGEP
                     VGSSKQASSAIDAVPDDEPIAFTVRAAGETHDVSLTREACAKNDPERYVGISTVNPFP
                     IPVSISSGDIGGPSAGLMFSLGLYDALTPGDLTDGRTIAGTGTMAPDGTVGPIGGIAD
                     KVVAAERVGATVFLVPKDNMAELEGVDVGDMQLISVATFDQAITELEAL"
     gene            1084..1509
                     /locus_tag="VIX62_11930"
     CDS             1084..1509
                     /locus_tag="VIX62_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860986.1"
                     /translation="MGFLIVIAIAATVGVLVYRLTDGGEPAAATIEDGPPPESAATAT
                     AGGQTAAATAPSDVEEWAGGDVDSPGRARSRPSPGPRRVELSPEAGSIPVVGARPSWH
                     SRLGGALGLLVAVGLGAIAIAGTLYLAGTMIARLISNAG"
     gene            complement(1526..>2206)
                     /locus_tag="VIX62_11935"
     CDS             complement(1526..>2206)
                     /locus_tag="VIX62_11935"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5860987.1"
                     /translation="RAIEEAVKFAGVRQVTLVEEPLAAAIGAGLPVHEPVGNLVVDIG
                     GGRSEMAVVSMGGVVSGRSAPVGGFDLDEAVQRHIKDEYGVAIGEKAAEEIKIAIGSA
                     FPTPTSKAAVVTGRELATGNTVEVRIDEAEIRQAIGVPIRGIVDAARHTLAEAPPELT
                     HDVLETGMFLTGGGSLLRGIDMLLAQECEVPVHVVERPLETVAVGTGRMLEHLEDYRS
                     AFQLVRRR"
BASE COUNT          304 a          769 c          782 g          351 t
ORIGIN      
        1 ccagggcctc gagttccgtg atcgcctggt cgaacgtggc gacggagatc agctgcatgt
       61 cgccgacgtc gacgccttcg agttcggcca tgttgtcctt cggcaccagg aacacggtgg
      121 ccccgacgcg ctcggcggcc acgaccttgt cggcgatccc gccgatcggg cctacggtgc
      181 cgtcgggtgc catcgtcccg gtgccggcga tggtgcgacc atccgtgagg tcgcccgggg
      241 tgagggcgtc atagaggccc agggagaaca tgagcccggc cgacgggccc ccgatgtcgc
      301 cgctggagat cgacaccggg atggggaacg ggttgaccgt gctgatcccc acgtaccgct
      361 ccgggtcgtt cttcgcacac gcttcccggg tgagcgagac gtcgtgggtc tcccccgccg
      421 cccgtacggt gaaggcgatg ggttcgtcat ccggcaccgc gtcgatcgcg ctgctcgcct
      481 gcttgctcga ccccaccggc tccccgccga tcgccgtgat caggtcaccg gggaagagct
      541 cgtcgaaggc ggggcagcct tccacggtgg cctggatcaa cgcgccatcg gcgtgctcct
      601 tggggtagtc cgtgaggtgc tgcatgacga cgtaggtggc gttgatcttg ctggtgtcca
      661 tctcggagat cgcccgttgg cgttcggtgg agggatcctc gccgggcgcg aacaggacgt
      721 cccggtgcac cacctgcgtg acggggtcga accaggcccc gagcgcctgc agcgccgtca
      781 cctgggcgta gctcacggtg gtcatgatga gcttgcccgc ggagtcgtag cgctggtggc
      841 ccttgacgtc gatcaacggt cccacctcgc gagcgggacc aggtcccacc gcgtagtacg
      901 gcaggcggac gtagcacgcg aacaacgtga ggccgaacag ggcgaccagg gcggcgatgc
      961 ggatcgggcg tttcatgggc gcaccgatac tacgggcggc cgtctcgaac gcaggccgtt
     1021 gtggcttcac gcacccctgg cacaggggta ccctcgaacg gtgccgaccg caggggtgcc
     1081 gccgtgggtt tcctgatcgt gatcgcgatc gcggcgacgg tgggcgtcct ggtgtatcgc
     1141 ctcaccgatg gtggcgaacc tgccgccgca acgatcgagg acgggccacc tccggagtcg
     1201 gcggcgacgg cgaccgcagg cgggcagacg gccgcggcca ccgcgccgtc cgacgtcgag
     1261 gagtgggcgg gtggcgacgt cgactcgccg ggcagggcac gttcccggcc ctcacccgga
     1321 cctcggcgcg tggaactctc gcccgaggcg ggctcgatcc cggtggtggg cgcccgtccg
     1381 tcgtggcact cgcgattggg tggcgcgctg ggtctgctgg tcgcggtggg cctgggggcg
     1441 atcgccatcg ccggaacgct ctacctggcg ggcacgatga tcgcccggct catctccaac
     1501 gccgggtagc gccccgccga cgcggtcagc gtcgccgcac caactggaag gccgagcgat
     1561 agtcctcgag gtgctccagc atccggccgg tccccaccgc gacggtctcc agcggccgct
     1621 ccacgacgtg gacgggtacc tcgcactcct gggccaggag catgtcgatg ccgcgcaaca
     1681 ggctcccgcc accggtgagg aacatgccgg tctccaggac gtcgtgcgtc agttcgggcg
     1741 gcgcctcggc gagggtgtgg cgcgcggcgt cgacgatgcc gcggatgggc acgccgatcg
     1801 cctgccggat ctcggcctcg tcgatgcgca cctccacggt gttgccggtc gcgagttcgc
     1861 gtccggtcac cacggccgcc ttgctggtcg gcgtggggaa cgccgaaccg atcgcgatct
     1921 tgatctcctc ggcggccttc tcgccgatcg ccacgccgta ctcgtccttg atgtggcgtt
     1981 gcacagcttc gtcgaggtcg aacccaccca ccggggcgga gcgaccgctg accacgccgc
     2041 ccatcgacac caccgccatc tccgaacggc cgccgccgat gtccaccacc aggtttccca
     2101 ccggttcgtg cacgggcaga ccggcgccga tcgcggcggc gagcggttct tcgaccaggg
     2161 tcacctggcg cacaccggcg aacttgacgg cttcttcgat cgcgcg
//
LOCUS       DATDBZ010000512         2484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1816125, whole genome shotgun sequence.
ACCESSION   DATDBZ010000512 DATDBZ010000000
VERSION     DATDBZ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816125"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VIX62_11940"
     CDS             <1..219
                     /locus_tag="VIX62_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY5860988.1"
                     /translation="QRWRNLHDDAVRSDPAAHAYVQALERDFDLAAEAAIPSADDLAA
                     DFEAFLRAHNEPPSAPDPAPDPDPDPDP"
     gene            complement(1174..>2484)
                     /locus_tag="VIX62_11945"
     CDS             complement(1174..>2484)
                     /locus_tag="VIX62_11945"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HEY5860989.1"
                     /translation="TAWGFETRQIHAGAAPDPATGARATPIYQTTSYQFRDTEHASNL
                     FALAEIGNIYTRIMNPTQGVLEGRINSLEGGCTTAVGLPGTLVVSSGQSAEILAILTL
                     AETGSHVVSSPSLYGGTYNLFHYTLPKLGIEVTFVDDPDDLEEWRAAIRPNTKVFFGE
                     TMSNPKNDVFDIEGVSKVAHDHGIPLMIDNTVPTPYLIRPLEWGADIVVHSLTKFLGG
                     HGTSIAGSITDGGTFDYGASGRFPNFIEPDPSYHGLAYWPALGHGSFILKARVQLLRD
                     LGMAISPFNAFLIIQGIETLSLRMERHFENAQKVADYLVARDEVESVAFAGLPSSAWH
                     DRATKYGRGKGYGSVIAFVLEGGKEAGQRFVEALELHSHVANIGDVRSLVIHPASTTH
                     SQLSEAEQASTGVHPGLVRLSVGIESIEDIIADLDAGFRAAKGG"
BASE COUNT          399 a          849 c          850 g          386 t
ORIGIN      
        1 cagcgttggc ggaatctgca cgacgacgcc gtgcgatccg acccagcggc gcatgcctac
       61 gtccaggcgc tcgagcgcga tttcgacctc gccgccgaag ccgccatccc gagcgccgac
      121 gacctggcgg ccgacttcga agcgttcctt cgcgcccaca acgaaccccc ctccgccccc
      181 gaccccgccc ccgatcccga ccccgacccc gacccgtgac ggccaacccc gactgacggc
      241 aaaccggggc ggcggccggg cccaccccga ctggccgggc cagctggtgc cggtggctcc
      301 tgtcacctga ggaggcatta cccagcgaat cgctgtcgaa tcgccgggta atgcggctga
      361 gggtggattg gtcgggtggg ggtggatcgg cgggggcgag gcgccagact gcgggcgttg
      421 acggcgttcg ccgctggcac acggtggagc accaccgctt gccgtgagcg accggcgtcc
      481 ggatcggtgt cctggctgac acccgacgct cgaagttcct gagctggagg tgtccccgtg
      541 cacgcacgac cagatggcgc actgccccct acgccgagat ggacgaagga cgacgaatgg
      601 cggctcgccc gggccaacgc cgggctcacg ttcacccatc gccacgcact cgatcgggaa
      661 cgccaggtcg tcgtcgatca gctcgacggg ctcgatcggg aggccggtca gctgatcgag
      721 cgccggcggg ccgttctcgc cgaggcccgc gacatccacc gagtgctgtg gcccccggcg
      781 acacgggact gggcgagacg accgccgcga ccggatgcgg cgccattggc gccgatggtc
      841 aacgacgccg aagaactgac cggctgggcg ctgcggtggg tcgccatggc gctgctgcgc
      901 cgtcacggtg aactcgaact gcgcgagatc cacgacctca tccatctcat gggctactgc
      961 gtcggtggcc gcaacccggt gaaggccctc ggcgacgcgc tcggccacga gcacgacaac
     1021 ggccgggcgg agcgggtccg gcgcggtgtg taccgcgtcg ccgaacccgt cccgcccaac
     1081 ccggtgtggg tgctccccga cccggagctg gcgctgatgc ctgcgtggtg gatcggtacc
     1141 tggtccgacc gataccacct cgccgaacgg cgctcagccg cctttcgcgg cccggaagcc
     1201 ggcgtcgagg tcggcgatga tgtcctcgat cgattcgatg ccgaccgaga gccggacgag
     1261 cccgggatgg acgccggtgc tcgcctgctc ggcctcggag agctggctgt gggtggtgga
     1321 ggcggggtgg atcaccaggc tgcgcacgtc accgatgttc gcgacgtgcg agtgcagttc
     1381 gagcgcttcg acgaaccgtt gcccagcctc cttcccaccc tcgaggacga acgcgatcac
     1441 cgagccatac cccttgccgc ggccgtactt cgtggcccgg tcgtgccagg cactcgaggg
     1501 caggccggcg aacgcgaccg actcgacctc gtcccgggca accaggtagt cggcaacctt
     1561 ctgcgcgttc tcgaaatgcc gttccatccg caggctgagc gtttcgatgc cctggatgat
     1621 gaggaaggcg ttgaacggcg atatcgccat gccgaggtcg cgcagcaact gcacgcgtgc
     1681 cttcaggatg aacgatccgt gcccgagcgc aggccagtag gcgaggccgt ggtacgaggg
     1741 gtcgggttcg atgaagttcg ggaagcgccc gctcgcgccg tagtcgaacg tgccgccgtc
     1801 cgtgatcgaa ccggcgatcg aggtgccatg gccgccgagg aacttcgtca gggagtggac
     1861 gacgatgtca gcaccccact cgagcggccg gatcaggtac ggcgtcggca cggtgttgtc
     1921 gatcatgagc gggatgccat ggtcgtgggc gaccttggac accccctcga tgtcgaacac
     1981 gtcgttcttc gggttcgaca tcgtctcgcc gaagaagacc ttcgtgttcg ggcggatcgc
     2041 cgccctccac tcttcgagat cgtcgggatc gtccacgaag gtgacctcga tgcccaactt
     2101 cggcaacgtg tagtggaaga ggttgtaggt gccgccgtac agggacggcg acgagaccac
     2161 atgactcccc gtctcggcga gggtgagaat cgccaagatc tcggccgact gcccggaact
     2221 cacgaccagg gtgcccggca gcccgacggc cgtcgtgcag ccaccctcca gcgagttgat
     2281 gcgcccttcc agcacgccct gcgtcgggtt catgatccgg gtgtagatgt tgccgatctc
     2341 cgccagtgcg aagaggttcg acgcgtgctc cgtgtcgcgg aactgataac tggtcgtctg
     2401 gtagatcggc gtcgcccgcg caccggtcgc cgggtcaggt gcagcacctg cgtggatctg
     2461 acgggtctcg aaaccccaag cggt
//
LOCUS       DATDBZ010000513         6344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_798574, whole genome shotgun sequence.
ACCESSION   DATDBZ010000513 DATDBZ010000000
VERSION     DATDBZ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6344
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798574"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VIX62_11950"
     CDS             <1..289
                     /locus_tag="VIX62_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY5860990.1"
                     /translation="GLGKYFLFGGYGSDSSVRSDLTRAAIARAEALHGHDVDPSEVMV
                     VGDTPRDVEAAHGAGAIAVGVATGEFTVGQLADAGADHVLNSFEEDDFPQL"
     gene            complement(308..3913)
                     /locus_tag="VIX62_11955"
     CDS             complement(308..3913)
                     /locus_tag="VIX62_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5860991.1"
                     /translation="MREERRVVTAVFADLVGSTALGERLDPEELKLVVGEAVSRMVVA
                     VEAFDGHVKDLAGDGVLAIFGAPTSHEDDAERAVRAGLRIVEEIAAFGLEIERSWGVA
                     EVDVRVGIETGPVVTGAIGGGSRVEYSALGDAVNTASRLQGQAVPGTVLVGAETHRSV
                     ADAFAWGPVRELTLKGKGSPVEAYPVAGVAGASPGPPRGRRVPMIGREAELTLADSLV
                     EGAVLGGSGAILYLSGEPGIGKTRMMQELRELVRGAQPAHGRSLWLEGRCVSYGESMS
                     YWPFRDLLRSWLGVAPDDPDLRVTIALRRAVDRLFGARAGELTPYLGALLELSFGPDA
                     DAKLAELSPEAAQFRTFEVVRHLIERLAEDGPVVMAFEDLHWADATSLLLLEQLVSDT
                     EALPLLIVCSGRPERDHPWWRLKDETARRLPHRFRAIALESLPDDSGSELLHALVGYD
                     TLPTSTAERVLASADGNPFFLEEIVRSLQDAGALVATDEGLRFDHEVDVEIPPTVEKV
                     ILARIDRLEPSPRQTLLAASVLGRRFSLSLLEAVAGDAGIRGALSELMRLDFVREGRR
                     WPEPEFRFKHALIQEAAYRTLVKDDRARLHRKAAESLEAQRGEGSRELAGLLAHHWLG
                     AADEDKAVRYLTLAGDRARQEYALDEAVAYYRELLPLLERRGEEHAIALGLFKLALAL
                     HMALRFSEADATYQRAFEHWTPPTPVDAEASVRFASSFLPNDPDPRSAIAWPNIQLCM
                     QLFDRLVEQWPERTIVPSLAERWDISADGLRYVFHLREGLRWSDGTPLTAGDVEFGIK
                     RVLDPSAPGSSVAVYFVLEHGQERYLGSPDEDRIGVRALDERTVEFRLAAPAPYFLSV
                     MNRPDGGPQPRHAIEADPGGWTRTGSQVVSGAFRLAERDDDRVLLERRDDYGGSRAGN
                     VSRVEILRSSLGEAVEAFKEGELDLVTVRYTPRMADVAPVDIADARLGPAAWSGYLAF
                     DHASGPTADVDLRLALAHAVDRDRLAEAVPINLVVATGGIVPPALQGHTPDVSLRFEP
                     DRAREALLRSGFAGPIAVACLDEDEVLLEPIVGSWREVLGLEVEMRVWDRASIASLPP
                     LRDVAPIYLTGWLPGYADPEYFLRLLFQSDSRTNEGGFASESFDLLIERARQERSDRS
                     RLELFHEADRMAVVDRVAVIPVVYGRSMALVSPRVQGWWEFGKSSANFAELNVDR"
     gene            complement(3972..4580)
                     /locus_tag="VIX62_11960"
     CDS             complement(3972..4580)
                     /locus_tag="VIX62_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5860992.1"
                     /translation="MPRTGGEERRVTIVEEAIRQIGRDGYRGASLDNIATAVGVRKQT
                     LLYYFPTKDALLEACLSAAGERLAREIAGALAGKESYWDRAEAVIHAVFRLASDWPEF
                     PMFIREAGRLGPDAFDRFAGVLDPMRQRAIDFLQAGMDNGEIRKQDPALLLFTLYTAV
                     VGSLTEASVLNAVVGADSSRASLRKREREVVLFVRAALRPVD"
     gene            complement(4584..5495)
                     /locus_tag="VIX62_11965"
     CDS             complement(4584..5495)
                     /locus_tag="VIX62_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5860993.1"
                     /translation="MAGMREELKTMAEGFRWGRRPLVPRSAEPFAPEKEDSTFPTAWA
                     RTEAGTAARQLILQSAMGPLLRSQVGLRVYGLEHLEGVAGPVIFFSNHTSHLDATVIM
                     TSLPNRWQAKTAVGAARDYFFDVWWRQAFTALVYGAFAIDRAGGGRKAVSQARELVDD
                     GWSLVVFPEGARSPDGHMQRFRHGTARLCIEAGIGAVPIGIRGAHQAMPKGRNWPRKG
                     KPPVRVRYGPPLFPAEGETHQAFSLRMTQAVAELHDEDRSTWWESLHRAQRGETPTLL
                     GPSGPEWRRKWEGSRPLERHGPDKTWK"
     gene            complement(5505..>6344)
                     /locus_tag="VIX62_11970"
     CDS             complement(5505..>6344)
                     /locus_tag="VIX62_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY5860994.1"
                     /translation="SGVKIFQIETTLNSETFPSNLGFLNKREWEWSTSDQGLMMAAKK
                     ANDLAPPRIRREFFQRIEAPYKLTGVNAGEVEAVHERTMTNLHRQQLVEVNGQADVLI
                     LGLPYLGPYNVNSILNPILVHCLGLGYLFNMYRNKPLVREGGVVILHHPVPWEFNQIH
                     HPSYIDFFEEVLRETTDPKEIEATFEERYATDPWYRHLYRTSHAYHGVHPFYMWYWGA
                     HGRDHAGDVIFVGGNPSSVARLGYRRADSMRDAIEMAKDTVGSSPTMTYFHAPPVMIA
                     DVR"
BASE COUNT         1042 a         2270 c         2074 g          958 t
ORIGIN      
        1 gggtttgggc aagtacttcc tgttcggggg gtacggcagc gattcatccg tccgaagcga
       61 cctcacccgc gccgcgatcg cacgcgcgga ggccctgcac ggacacgacg tcgatccctc
      121 cgaggtcatg gtcgtaggtg acacaccgcg tgacgtggaa gccgcgcacg gggccggggc
      181 catcgcggtc ggggtcgcca ccggcgaatt cacggtgggc caactcgccg acgcgggtgc
      241 cgaccacgtc ctcaactctt tcgaagagga cgatttccct cagttgtgac cacggacgat
      301 ccgcagttca acgatccacg ttgagctcgg cgaagttcgc cgaactcttg ccgaactccc
      361 accacccctg gacgcggggt gacaccagcg ccatgcttcg gccatagacg acggggatca
      421 cggcgacccg atcgacgacc gccatccggt cggcctcatg gaacaactcg aggcggctcc
      481 ggtcgctccg ttcttgtcgc gcccgctcga tcagcagatc gaaggactcc gacgcgaacc
      541 caccttcgtt cgtcctgctg tcggactgga agagcaatcg aaggaagtac tcggggtctg
      601 catagccagg cagccatccg gtgaggtaga tcggggcgac atcgcgaagt ggcgggagcg
      661 atgcgatcga tgctcggtcc cagacccgca tctcgacctc cagtccgagc acctcgcgcc
      721 aggaccccac gatcggctcg agcaacacct cgtcttcgtc gagacatgcg accgcgatcg
      781 gcccagcgaa tcccgaacgc agcagggcct cccgagcccg atccggctcg aaccgcaacg
      841 agacatcggg cgtgtgtccc tggagcgcag gaggcacgat gccgccggtg gcaaccacga
      901 ggttgatcgg tacggcctcg gcgagccggt cccgatcgac ggcatgggcc aacgcgagcc
      961 gcagatcgac atcggcggtc ggaccagacg catggtcgaa cgccaggtat cccgaccacg
     1021 cggcaggtcc caacctcgca tcagcgatgt ccacgggagc cacgtcagcc atcctcggcg
     1081 tataccggac ggtgacgaga tcgagctcgc cttccttgaa ggcctcgacc gcctccccca
     1141 gggacgaacg aaggatctcg acgcggctga cgttgccggc acgggagccg ccgtagtcgt
     1201 cccgacgttc caggaggacg cggtcatcgt cccgctcggc caagcgaaag gcacccgaca
     1261 cgacctgact gccggtgcgc gtccatccgc cgggatcggc ctcgatcgcg tggcgcggct
     1321 gtggaccacc gtcgggccgg ttcatcacgc tcaggaagta gggcgccgga gccgccaatc
     1381 ggaactccac cgtccgttcg tccaatgccc gcacgccgat ccggtcttcg tcgggcgacc
     1441 ccaggtagcg ctcttgaccg tgttcgagca cgaagtagac cgcaaccgat gaacccggcg
     1501 ccgacggatc gagcacccgt ttgatgccga actccacatc accggccgtc agcggcgtgc
     1561 cgtccgacca cctgaggcct tcccgaaggt ggaagacgta gcgcaggccg tcggcggaga
     1621 tgtcccaacg ttccgcaagg gaggggacga tcgtgcgctc gggccattgc tctacgaggc
     1681 ggtcgaacaa ctgcatgcac agctggatgt tcggccaggc gatcgccgat cgagggtcgg
     1741 ggtcgttcgg caggaagcta ctggcgaagc gcacggaggc ctccgcgtcg accggtgtcg
     1801 gaggggtcca atgctcgaac gctcgctggt aggtggcgtc ggcttccgag aaccggagcg
     1861 ccatatgcag cgcgagggcc agcttgaaca acccgagggc gatcgcgtgc tcctcgccgc
     1921 gtcgctcgag cagaggcaac agctcgcggt agtaggcgac cgcttcatcc agtgcgtact
     1981 cctgacgggc acggtcgccg gcgagcgtga ggtatcggac ggccttgtcc tcgtcggcgg
     2041 ctccgagcca gtggtgagcg agcagtccgg ccagctcgcg cgacccctcc cctcgctggg
     2101 cctccaatga ctcagcagcc ttgcggtgca atcgtgcgcg gtcgtccttc accagcgttc
     2161 ggtacgccgc ctcttggatc agcgcgtgct tgaaccggaa ctcgggttcg ggccaacggc
     2221 gaccctcacg gacgaagtcc agtcgcatca gctccgagag cgctccacgg atgccggcat
     2281 ctcccgcgac ggcctccaac agcgacaggc tgaaccgtcg gcccaggacc gaggcggcga
     2341 gcaacgtctg gcgcggcgac ggttcgagcc ggtcgatccg ggccaagatc accttctcca
     2401 ccgtgggcgg gatctccaca tcgacctcgt ggtcgaaccg cagaccctcg tcggtggcca
     2461 cgagcgcacc ggcgtcctgc aacgagcgca cgatctcctc caggaagaag gggttcccgt
     2521 cggccgacgc gaggacacgt tcggccgtgc tcgtcggcaa cgtgtcatag cccacgagag
     2581 cgtggagcaa ctccgatccc gaatcgtcgg gaagggactc caaggcgatc gcccggaacc
     2641 ggtgcggcag ccggcgggcc gtttcgtctt tcagtcgcca ccaagggtgg tcacgctccg
     2701 gacgcccgct gcacacgatc agcagcggga gcgcctcggt atcgctgacc agctgttcca
     2761 gcagaaggag cgacgtggca tccgcccaat gcagatcctc gaacgccatc accacgggcc
     2821 cgtcctcggc gaggcgctcg atcaggtggc gcacgacctc gaacgtccgg aactgtgctg
     2881 cctcgggcga gagctcggcg agcttggcat cggcgtccgg accgaacgac agctccagga
     2941 gcgcgccaag gtagggggtg agttcgccgg cgcgcgcgcc gaacagccgg tccaccgcgc
     3001 ggcgcaacgc gatcgtgaca cgaaggtcgg gatcgtcggg cgctacgccc agccaggacc
     3061 ggaggaggtc gcggaacggc cagtaggaca tcgactcgcc gtagctgacg caccggcctt
     3121 cgagccagag cgaacggccg tgcgcgggct gtgcccctcg gaccagctca cggagctcct
     3181 gcatcatccg ggtcttgccg atcccgggct cccccgacag gtagaggatg gcgccggagc
     3241 ccccgagcac ggccccctcc accagggagt cggccagcgt cagctcggct tcccggccta
     3301 tcatcggcac ccggcgcccg cggggcggcc ccggcgaggc accggcgaca cctgccacgg
     3361 gataggcctc gacgggtgat cctttgccct tcaaggtgag ctcccgcacc ggtccccacg
     3421 cgaacgcatc cgccaccgac cgatgggtct ccgcgcccac cagcacggtg ccgggcaccg
     3481 cctggccctg cagccgggat gcggtgttga ccgcgtcccc gagcgccgag tactccacgc
     3541 ggctcccgcc gccgatcgcg ccggtcacga ccgggccggt ctcgatcccc acgcggacgt
     3601 ccacctcggc gacaccccag gaccgctcga tctccagccc gaacgccgcg atctcttcga
     3661 cgatccgcag cccagcacga acggctcgtt ccgcatcatc ctcgtgcgag gtcggagccc
     3721 cgaagatcgc cagcacgccg tcgcccgcga ggtccttcac gtggccgtcg aacgcctcga
     3781 ccgcgaccac catgcgggag accgcctcgc ccaccacgag cttcagttcc tcggggtcca
     3841 gccgttcacc gagcgccgtc gagccgacca ggtcggcgaa gaccgcggtc acgacccgcc
     3901 gttcctcgcg catgctcacc gctgcatcct ccgtcaaggg accggcgagc gacaatcccg
     3961 gaggccgcgt ctcaatccac cggtcgcaac gcggcgcgca cgaacagcac cacctcgcgc
     4021 tctcgcttgc gcaacgacgc gcgactggag tcggcgccca ccaccgcgtt caggacgctc
     4081 gcctcggtga gcgagcccac caccgccgtg tacagcgtga acagcagcag cgccggatcc
     4141 tgtttgcgga tctcgccgtt gtccatcccg gcctgcagga agtcgatcgc gcgttggcgc
     4201 ataggatcga gcaccccggc gaatcggtcg aacgcatccg gcccgagccg gccggcctcg
     4261 cggatgaaca tcgggaactc cggccagtcg ctcgcgagcc ggaagacggc gtggatcacc
     4321 gcctcggcgc ggtcccagta gctctccttg ccggctaacg ccccggcgat ctcgcgagcg
     4381 aggcgctccc ctgccgcgga caggcacgcc tcgagcaacg cgtccttcgt ggggaagtag
     4441 tagaggagcg tctgcttgcg cacccccacc gccgtcgcga tgttgtcgag gctggcgcca
     4501 cgatagccgt cgcgtccgat ctgccggatc gcctcttcga cgatcgtgac tcgacgttcc
     4561 tcaccgccgg tgcgtggcac cgctcacttc caggtcttgt cggggccgtg ccgctcgagc
     4621 ggacgcgagc cctcccactt gcgacgccac tcggggccgg agggacccag cagcgtgggg
     4681 gtctcgccgc gctgagcgcg gtgcagcgac tcccaccacg ttgaacggtc ctcgtcgtgg
     4741 agctcggcca ccgcctgcgt catccgcagc gagaacgcct ggtgcgtctc gccttccgcg
     4801 gggaacaacg gcgggccgta gcgaacccgc accggtggct tgcccttccg cggccagttc
     4861 ctgcccttcg gcatggcttg gtgggcacca cggatcccga tcggcaccgc gccgatgccg
     4921 gcctcgatgc acagacgggc cgtgccgtgc cggaaccgct gcatgtggcc gtcgggcgac
     4981 cgcgcgccct cggggaacac cacgagcgac catccgtcgt cgaccagctc gcgcgcctgg
     5041 ctcaccgcct tgcgcccgcc gcccgcgcgg tcgatcgcga acgcgccgta caccaacgcg
     5101 gtgaacgcct gccgccacca cacgtcgaag aagtaatcgc gcgccgcgcc gaccgcggtc
     5161 ttggcctgcc agcggttcgg gagcgaggtc atgatcaccg tcgcatccag atgactcgtg
     5221 tgattggaga agaagatcac cgggcccgcc acgccctcga ggtgttcgag gccgtagacc
     5281 cgcaggccca cctggctccg cagcaggggt cccatcgcgc tctgcaggat cagctgtcgc
     5341 gccgccgtgc cggcctcggt ccgtgcccac gcggtcggga aggtcgagtc ttccttctcg
     5401 ggtgcgaacg gttcggccga tcgcggcacc agcggacgac ggccccagcg gaagccttcc
     5461 gccatggtct tgagctcttc gcgcatgcct gccatcgccg tctcctaccg cacgtccgcg
     5521 atcatcacgg gcggcgcatg gaagtaggtc atcgtcggcg acgagcccac ggtgtccttc
     5581 gccatctcga tcgcgtcgcg catcgagtcc gcgcgccggt agccgagccg cgccacgctc
     5641 gaggggttcc cgccgacgaa gatcacgtcg ccggcgtggt cgcgaccgtg cgcgccccag
     5701 taccacatgt agaacgggtg cacgccgtgg taggcgtgac tggtccgata caggtggcgg
     5761 taccaggggt cggtggcgta ccgctcctcg aaggtcgcct cgatctcctt cgggtcggtg
     5821 gtctcgcgca gcacctcttc gaagaagtcg atgtagctgg ggtggtggat ctggttgaac
     5881 tcccacggca ccgggtggtg caggatcacg acgccgccct cacgcaccag cggcttgttc
     5941 cggtacatgt tgaacaggta gcccagcccc aggcagtgca cgaggatcgg gttgaggatc
     6001 gagttcacgt tgtaggggcc gaggtagggc agacccagga tcaggacatc ggcctgaccg
     6061 ttcacctcga ccagctgctg gcgatgcagg ttcgtcatcg tgcgctcgtg gacggcttcg
     6121 acctcgccgg cgttgacgcc ggtcagcttg tagggcgcct cgatccgctg gaagaactcc
     6181 cggcggatgc gcggtggcgc gaggtcgttc gccttcttgg ccgccatcat caggccctgg
     6241 tcggacgtgc tccactccca ctcgcgcttg ttcaggaagc ccaggttcga cgggaacgtc
     6301 tcggagttca gggtggtctc gatctggaag atcttgaccc cgct
//
LOCUS       DATDBZ010000514         3038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_375092, whole genome shotgun sequence.
ACCESSION   DATDBZ010000514 DATDBZ010000000
VERSION     DATDBZ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375092"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VIX62_11975"
     CDS             <1..1446
                     /locus_tag="VIX62_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860995.1"
                     /translation="RARGVVVVGFGVVIVFGIVLAGIDAGYRSVLDIPFDPLDWQQLG
                     DAFGVVQGSIGGAAAGWMVVLLAIAAAGSIVALSWAALRVGSAVGPDRAHGPIVISAV
                     TAAWALAALTGSQPVAGQPAAAAASLHAITASASHAAAGLAALADLPQQIATDPYRDT
                     PGSDLLTSLKGKDVVFAFIESYGRVAVEGSSLSGGVDQVLRDGEAQLRADGYESQSAF
                     LTSPTFGGLSWLAHSTFQSGLWVDRQPLYSELIRSDRFTLSEAFGRAGWRTVSVVPSN
                     SEPWSFGRSFYRFDTLLDANNVGYRGPSFGYARIPDQYTWKHFADSELAAGHQPVMAE
                     IDLVSSHTPWAPLPELVPWSQVGDGSVFEPQPAEGESATTVWQDPKRVRQAYAESIRY
                     SLGAMFAFLHESDDPNLVLVVLGDHQPATIVSGHGAGHDVPISIISKDSTVFESIEGW
                     LWHDGMRPAPDAPVWPMSAFRDRFLDAFSAR"
     gene            complement(1433..1678)
                     /locus_tag="VIX62_11980"
     CDS             complement(1433..1678)
                     /locus_tag="VIX62_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005514534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEY5860996.1"
                     /translation="MATKKPQKWSDLSSRRQTGMIIGGVIQLALAATAWTDLARRPAD
                     KVNGPKWLWAVLIAVNFVGPLSYFVFGRTRGAELIEH"
     gene            1884..>3038
                     /locus_tag="VIX62_11985"
     CDS             1884..>3038
                     /locus_tag="VIX62_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002754671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY5860997.1"
                     /translation="MTADRHARSMLPIPDRPAPGLTTYDAKDPDTSYPPIQPLLPPEG
                     APNVLVILIDDVGFGASSAFGGPCATPNAERLAAGGLKFNRFHTTALCAPTRQALLTG
                     RNHHSVGMGSITETATSAPGNSSLRPNTKAPLAMTLKLNGYSTAQFGKCHEVPVWQSS
                     PMGPFDAWPSGGGGFETFYGFIGGENNQWDPALYEGTTPVEPPATPEEGYHLTEDLAD
                     HAIGWIRSQKALMPDRPFFVYFAPGATHAPHHVPKEWADKYAGKFADGWDTQRERTFA
                     RQKELGVIPADAELTPRHNEIPAWDDMPADLKPVLEREMEVYAGFLEHTDHHVGRVLD
                     AIEDLGVLENTLIYYIIGDNGASAEGTLNGAFNEMANFNGMAALESPEFMA"
BASE COUNT          461 a         1074 c         1016 g          487 t
ORIGIN      
        1 cgcgcgcgtg gtgtcgtcgt ggtcgggttc ggcgtcgtca tcgtcttcgg gatcgtgctc
       61 gccgggatcg acgccggcta ccgctccgtg ctcgacatcc cattcgatcc gctcgactgg
      121 cagcagctgg gcgacgcgtt cggggtcgtc cagggttcca tcggcggcgc cgccgcaggc
      181 tggatggtcg tgctcctcgc gatcgccgcc gccgggtcga tcgtcgcgct ttcctgggcc
      241 gcgcttcgcg tgggctccgc cgtcgggccc gatcgcgcgc acggaccgat cgtgatctcg
      301 gcggtcactg cggcatgggc tctcgccgcc ctgacgggct cgcagccggt ggcgggtcag
      361 ccggccgcgg cggcggcctc gctccacgcg atcaccgcgt cggcctccca cgccgccgcg
      421 gggctggcgg cgctggccga cctgccgcag cagatcgcga cggaccccta ccgggacaca
      481 cccggctccg acctgctgac ctcgctgaaa ggcaaggacg tcgtcttcgc cttcatcgag
      541 agctacggga gggtggcagt ggagggatcc agcctctcgg ggggtgtgga ccaggtgctg
      601 cgcgacggcg aggcccagct ccgcgccgac ggctacgaat cccagagcgc cttcctcacg
      661 tctccgacat tcggcgggct cagctggctc gcgcactcga ccttccagtc cggcctctgg
      721 gtcgaccggc agccgctcta cagcgagctg atccgaagcg atcgattcac cctgagcgag
      781 gcattcggac gagccgggtg gcgcacggtg agtgtggtgc cctcgaacag tgagccatgg
      841 tcgttcggca ggtcgttcta ccgcttcgac accctgctcg atgcgaacaa cgtcggatat
      901 cgggggccgt ccttcgggta tgcgcggatc ccggaccagt acacgtggaa gcacttcgcc
      961 gacagcgagc tcgccgccgg ccatcagccg gtcatggccg agatcgacct cgtctcctcg
     1021 cacacgccct gggcgccgct ccccgagctc gtgccctggt cccaggtggg cgacggttcc
     1081 gtgttcgagc cccaacccgc ggagggggag tcggcgacca ccgtctggca ggacccgaag
     1141 cgggtgcggc aggcgtacgc cgagtccatc cggtattcgc tcggtgcgat gttcgcgttc
     1201 ctgcacgagt ccgacgatcc gaacctcgtg ctggttgtgc tcggcgacca ccagccggcg
     1261 acgatcgtga gcgggcacgg cgccggccac gacgtgccga tcagcatcat ctcgaaggac
     1321 agcaccgtct tcgagtcgat cgaggggtgg ctctggcacg acgggatgcg cccggcgccc
     1381 gacgcgcccg tctggccgat gagcgcgttc cgcgatcgat tcctcgacgc cttcagtgct
     1441 cgatgagctc tgcgccccgc gtacgcccga acacgaagta cgacagcggc ccgacgaagt
     1501 tcaccgcgat cagcaccgcc cagagccact tcggcccgtt caccttatcg gccgggcggc
     1561 gggcgaggtc ggtccacgcg gttgcggcga gcgcgagctg gatgactccg ccgatgatca
     1621 tccccgtctg cctgcgactg ctgaggtcac tccatttctg gggcttcttg gtcgccatgg
     1681 tcgtctcctc catcgacggc gtcgagtgat cgcgccgatt cggtgccacc ctagcgctgt
     1741 ggcatcccgg ccgcgacgag gcgctgggat gcgaccaggt cggccttggc atggcgcccc
     1801 ggacattcac ccggtctgtg tgatgcggca acacggcggg gcgatgaggc tggcgagcac
     1861 cgaccaacct gggggaattc gtcatgaccg ctgatcgaca cgcccgctcg atgctcccga
     1921 tcccggatcg accggcaccg gggctcacga cgtacgacgc gaaggacccg gatacctcgt
     1981 atccgcccat ccaaccgttg ctgccgcctg agggcgcgcc gaacgtcctc gtcatcctca
     2041 tcgacgacgt cggcttcggg gcatcgagcg cgttcggcgg tccgtgcgcg acgccgaacg
     2101 ccgaacgact cgcggccggc ggcctgaagt tcaaccggtt ccacaccact gccctctgtg
     2161 cgccgacccg tcaggcgctg ctcaccggtc gcaaccacca ctcggtcggc atgggcagca
     2221 tcacggagac ggcgacctcg gcgcccggga acagctcgtt gcggccgaac accaaggcgc
     2281 cgctcgccat gacgctgaaa ttgaacggct actcgaccgc acagttcggc aagtgccatg
     2341 aagtgccggt gtggcagtcc tctccgatgg gtcccttcga tgcgtggccg tcgggcggtg
     2401 gcgggttcga gacgttctac gggttcatcg gcggcgagaa caaccagtgg gaccccgccc
     2461 tctacgaagg cacgaccccg gtggagccgc cggcgacccc cgaggagggc taccacctca
     2521 ccgaggacct cgctgatcat gcgatcggct ggatccggtc gcagaaggcg ctgatgcccg
     2581 accggccgtt cttcgtgtac ttcgcgcccg gcgccaccca cgcgccccac cacgtgccca
     2641 aggagtgggc cgacaagtac gccgggaagt tcgccgacgg ctgggacacg cagcgtgagc
     2701 gcacgttcgc ccggcagaag gaactcggcg tcatcccggc cgacgccgag ctcacgccgc
     2761 ggcacaatga gatcccggcc tgggacgaca tgcccgccga tctgaagcca gtgctcgagc
     2821 gcgagatgga ggtctatgcg gggttcctcg agcacaccga ccatcatgtc ggccgcgtcc
     2881 tcgatgccat cgaggatctc ggcgtgctcg agaacacgct gatctactac atcatcggcg
     2941 acaacggggc atccgccgag ggcacgctga acggcgcgtt caacgagatg gcgaacttca
     3001 acgggatggc cgcgctcgag tcgcccgagt tcatggcg
//
LOCUS       DATDBZ010000515         2227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_995677, whole genome shotgun sequence.
ACCESSION   DATDBZ010000515 DATDBZ010000000
VERSION     DATDBZ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995677"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /gene="moaC"
                     /locus_tag="VIX62_11990"
     CDS             <1..438
                     /gene="moaC"
                     /locus_tag="VIX62_11990"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY5860998.1"
                     /translation="RIVEVGAKAETERVATAECWVTTTSEAVGHLVAGTKKGDPIQTA
                     KIAGVIGVKQTPALLPHCHPIRVTGADVVVEPDEASGRLRIETTVRATDRTGVEMEAL
                     TAAAVAALSLYDTAKAFDRAARIDGLRLLRKSGGKSGDYVADA"
     gene            649..1230
                     /locus_tag="VIX62_11995"
     CDS             649..1230
                     /locus_tag="VIX62_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5860999.1"
                     /translation="MEWLPLAVLGIMWVAFLLPTRGRTSTPQGSVGDFERRMELLAQA
                     ESHGTEGRWIVTPRKGVRFMGPTERNRARTRERRRQVFTFMLEAIGITFLIGLVPPLR
                     VAWAVTGVFVGLLAVYVWLLLTIKHRESTFRTAPHRHPKSHAAPTRPAPQPRYVAEGR
                     STRARPSFNGLGTLGEGDRVHVVVRVGEATAGA"
     gene            1241..1876
                     /locus_tag="VIX62_12000"
     CDS             1241..1876
                     /locus_tag="VIX62_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEY5861000.1"
                     /translation="MALDDLTRDLHTRFDATVAAREQALPAARRSIRASANAIRAVHR
                     GEFDRADELIEESRAAMQAGLDAVTDHPEVRFAGYLQDAQKEYAEANVTRALVAGDPL
                     PGPVELGVGDAPYLNGIAEAIGEGRRHILDLLRAGEVEGGQAMLDVLEDLYGVLVTMD
                     YPDAITMNLRRSTDVSRSLIEKTRGDLAIAAMQQGIERALDEHARRLQDDA"
     gene            1918..1991
                     /locus_tag="VIX62_12005"
     tRNA            1918..1991
                     /locus_tag="VIX62_12005"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1952..1954,aa:Ala,seq:cgc)
BASE COUNT          351 a          781 c          759 g          336 t
ORIGIN      
        1 cgcatcgttg aggtcggcgc gaaggccgag accgagcgcg tcgcgaccgc cgagtgctgg
       61 gtcacgacca cctccgaagc agtcggacat ctcgtcgccg gcacgaagaa gggcgatccg
      121 atccagaccg cgaagatcgc cggggtgatc ggcgtgaagc agacacctgc gctattgccg
      181 cactgccacc cgatcagggt caccggcgcc gatgtggtcg tcgagcccga cgaggcttcc
      241 ggcaggcttc gcatcgagac caccgtgcga gcgaccgacc gtacgggcgt cgagatggag
      301 gctctgacgg ccgccgctgt cgccgccctc tcgctttacg acaccgccaa ggcgttcgac
      361 cgtgcggccc gcatcgacgg tctgcggctc ctgaggaaat cgggcggcaa gagcggcgac
      421 tacgtcgccg acgcctgatc cacgtcgctg tagcccggcc ggggagttcc ttcctccgtc
      481 aaccggcata cagcgtccga cgtgaacgcg cggtgtcggg acggcggctc tgcgaaaaaa
      541 gttcgaatca cacggatgtg atcgcttcac cccatatgtg gtgctagcgt cccggcccag
      601 cctagatgtg gtagcgccga tagtcaccga ccccacggga ggctgggcgt ggagtggttg
      661 cccctcgcgg tcttgggcat catgtgggtg gcgttcctgc tccccaccag aggcaggacg
      721 tcgacccccc aggggtccgt cggggacttc gaacgcagga tggagctatt ggcgcaggct
      781 gagtcgcacg gcaccgaggg ccgttggatc gtcactcccc gcaagggcgt gcggttcatg
      841 ggacccaccg aacggaaccg ggctcgcacc cgggagcgcc gccgacaggt cttcaccttc
      901 atgctcgagg cgatcggcat cacgttcctc atcgggctcg tgccgccgct ccgggttgca
      961 tgggccgtca cgggtgtgtt cgtcgggctg ctcgccgtct acgtctggct gctgctcacg
     1021 atcaagcacc gcgagtccac cttccgcacc gccccgcacc gtcacccgaa gtcccacgcc
     1081 gcgccgacac ggccggcgcc ccagccccgg tacgtggccg agggacgcag cacccgggcg
     1141 cgaccgagct tcaacgggct cggcacgctc ggcgagggcg accgcgtgca cgtggtcgtg
     1201 cgcgtgggcg aggccaccgc cggcgcctga ccggttcggc gtggccctcg acgatctcac
     1261 ccgcgacctc cacacccggt tcgacgccac cgtcgccgcg cgcgagcagg cgctccctgc
     1321 cgcacgtcgt tcgatccgtg cgagcgccaa tgcgatccgt gccgtgcacc ggggtgagtt
     1381 cgatcgcgcc gacgagctga tcgaggagtc gcgcgccgcg atgcaggccg gcctcgacgc
     1441 ggtcaccgac caccccgagg tgcggttcgc cggttacctg caggacgccc agaaggagta
     1501 cgccgaggcg aacgtcacgc gggcgctcgt cgccggcgac ccgctgcctg ggccagtcga
     1561 gctcggcgtc ggagacgcgc cctacctgaa cggcatcgcc gaggcgatcg gggaaggtcg
     1621 ccgccacatc ctcgacctgc tgcgcgccgg cgaggtcgag ggcggccagg cgatgctcga
     1681 cgtactcgag gatctctacg gtgtgctggt cacgatggac taccccgacg cgatcacgat
     1741 gaacctgcgg cgctccacag acgtctcccg gtccctgatc gagaagaccc gcggcgacct
     1801 cgcgatcgcc gcgatgcagc agggcatcga gcgagcgctc gacgagcacg cgcgccgcct
     1861 tcaggacgac gcctagctgt cggccgtggc agaccccttt gctacgctgg ctctcctggg
     1921 ggtgtagctc agcccggtag agcgctgcct tcgcacggca gaggtcaggg gttcgaatcc
     1981 cctcacctcc acccagggga acgcgggtca ggactcggtt gccaacaggg tggggaccgc
     2041 tccaggtcct agagctgcag tcatcgaccg actgcgcggg tcacggacaa agaggaacaa
     2101 tggttggcag cgggagttct ggatcggcgg aggccgtgaa ccctccggcg gccgacccgt
     2161 tcttgtatgg catctggctg ttgtgcagcg ccgcgtgacc ccgggaccgg ttcccttggc
     2221 gaccaca
//
LOCUS       DATDBZ010000516         2060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1616668, whole genome shotgun sequence.
ACCESSION   DATDBZ010000516 DATDBZ010000000
VERSION     DATDBZ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1616668"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1352)
                     /locus_tag="VIX62_12010"
     CDS             complement(33..1352)
                     /locus_tag="VIX62_12010"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HEY5861001.1"
                     /translation="MSETAWGFETRQIHAGAAPDPATGARATPIYQTTSYQFRDTEHA
                     SNLFALAEIGNIYTRIMNPTQGVLEGRINSLEGGCTTAVGLPGTLVVSSGQSAEILAI
                     LTLAETGSHVVSSPSLYGGTYNLLHYTLPKLGIEVTFVDDPDDLEQWRAAIRPNTKVF
                     FGETMSNPKNDVFDIEGVSKVAHDHGIPLMIDNTVPTPYLIRPLEWGADIVVHSLTKF
                     LGGHGTSIAGSITDGGTFDYGASGRFPNFIEPDPSYHGLAYWPALGHGSFILKARVQL
                     LRDLGMAIAPFNAFLIIQGIETLSLRMERHFENAQKVADFLVARDEVESVAFAGLPSS
                     AWHDRATKYGRGKGYGSVIAFVLKGGKEAGQRFVEALELHSHVANIGDVRSLVIHPAS
                     TTHSQLSEAEQASTGVHPGLVRLSVGIESIEDILADLDAGFRAAKGG"
     gene            complement(1386..>2060)
                     /gene="ilvC"
                     /locus_tag="VIX62_12015"
     CDS             complement(1386..>2060)
                     /gene="ilvC"
                     /locus_tag="VIX62_12015"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY5861002.1"
                     /translation="AAPAGVDVIMVAPKGPGHLVRRTYTEGGGVPALIAVEQDSTGNA
                     KALALSYADAIGGTRAGVIETTFPEETETDLFGEQVVLCGGLTSLVQAGFETLVDAGY
                     APEMAYFECLHEVKLIVDLMYEEGIAGMRYSISDTAEYGDLTRGPRIITDATKAEMRT
                     ILDEIQSGKFADEWVAESESGRANYKALQEKGRQHPIEEVGAMLRGMMPWISAGRTKV
                     AEASGG"
BASE COUNT          320 a          694 c          683 g          363 t
ORIGIN      
        1 ggtccgaccg gtaccacctc gcaaaggggc gctcagccgc ctttcgcagc ccggaagccg
       61 gcgtcgaggt cggcgaggat gtcctcgatc gattcgatgc cgaccgagag ccggacgagc
      121 ccgggatgga cgccggtgct cgcctgctct gcctcggaga gctggctgtg ggtggtggag
      181 gcggggtgga tcaccaggct gcgcacgtca ccgatgttcg cgacgtgcga gtgcagttcg
      241 agggcttcga cgaaccgttg cccggcctcc ttcccaccct tcaggacgaa ggcgatcaca
      301 gagccatacc ccttgccgcg gccgtacttc gtcgcccggt cgtgccaggc gctcgacggc
      361 agcccggcga acgcgaccga ctcgacctcg tccctggcca ccaggaagtc ggcaaccttc
      421 tgcgcgttct cgaaatgccg ttccatccgc aggctgagcg tctcgatgcc ctggatgatg
      481 aggaacgcat tgaagggcgc tatcgccatg ccgaggtcgc gcagcaactg cacgcgtgcc
      541 ttcaggatga acgatccgtg cccaagcgcg ggccagtagg cgaggccgtg gtacgagggg
      601 tccggctcga tgaagttcgg gaaacgcccg ctcgcgccgt agtcgaacgt gccgccgtcc
      661 gtgatcgaac cggcgatcga ggtgccgtgg ccgccgagga acttcgtgag ggagtggacg
      721 acgatgtcgg cgccccactc gagcgggcgg atcaggtacg gcgtcggcac ggtgttgtcg
      781 atcatgagcg ggatgccgtg gtcgtgggcg accttggaca ccccttcgat gtcgaacaca
      841 tcgttcttcg ggttcgacat cgtctcaccg aagaagacct tcgtgttcgg gcggatcgcg
      901 gcccgccact gctcgaggtc gtcgggatcg tccacgaacg tgacctcgat gcccagcttc
      961 ggcaacgtgt agtggaggag gttgtaggta ccgccgtaga gcgacggcga ggagaccacg
     1021 tggctccccg tctcggcgag ggtcaggatc gccaggatct cggccgattg tccggaactg
     1081 accaccagcg tgcccggcag cccgacggcg gtcgtgcagc caccctcgag cgagttgatg
     1141 cgcccttcga gcacgccctg cgtcgggttc atgatccggg tgtagatgtt gccgatctcc
     1201 gccagggcga agaggttcga tgcgtgttcc gtgtcgcgga actggtagct ggtcgtctgg
     1261 tagatcggcg tggcgcgcgc gccggtcgcc ggatcaggtg cagcacctgc gtggatctga
     1321 cgggtctcga aaccccaagc ggtctcgctc attgcagcct ccttgaacgt ggccgcggca
     1381 gtctctcatc cccccgaggc ttcggccacc ttggtgcgcc cggccgagat ccacggcatc
     1441 atgccccgca gcatcgcacc gacctcctcg atgggatgct ggcgcccctt ctcctgcagc
     1501 gccttgtagt tcgcccggcc cgactcgctc tccgcgaccc actcgtcggc gaacttgccc
     1561 gactggatct cgtccagaat cgtcctcatc tccgccttgg tggcatcggt gatgatccgt
     1621 ggaccgcggg tcaggtcccc gtactccgcc gtgtcggaga tcgagtagcg catgccggcg
     1681 atgccttcct cgtacatgag gtcgacgatg agcttcacct cgtggaggca ctcgaagtac
     1741 gccatctcgg gcgcgtatcc cgcgtcgacg agggtctcga agcccgcttg caccaacgac
     1801 gtcaggccgc cgcagagcac gacctgctcg ccgaacaggt cggtctcggt ctcttccggg
     1861 aacgtcgtct cgatgacccc ggctcgcgtc ccaccgatgg cgtcggcgta ggacagcgcc
     1921 agcgccttcg cgttaccggt cgagtcctgc tcgaccgcga tcaacgccgg gacaccgccg
     1981 ccctcggtgt acgtacggcg cacgagatgg ccggggccct tcggcgccac catgatcaca
     2041 tcgacgccgg cgggcgccgc
//
LOCUS       DATDBZ010000517         2950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_376080, whole genome shotgun sequence.
ACCESSION   DATDBZ010000517 DATDBZ010000000
VERSION     DATDBZ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376080"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VIX62_12020"
     CDS             complement(<1..315)
                     /locus_tag="VIX62_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861003.1"
                     /translation="MEPETVHVGATERAPAEAQMAAAQAPPVVAVVVVHRPEAWFEDT
                     VEAFARQDYPNLRFLFLVAGDGVADTDTAFDAVEDRITARLPGAFVRPVAGNPGFGPV
                     ANE"
     gene            400..621
                     /locus_tag="VIX62_12025"
     CDS             400..621
                     /locus_tag="VIX62_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861004.1"
                     /translation="MAPRPRFDPTSLDLTDFDVSAMLDSDAGRAARTVVGAAATGVRE
                     VTYVTVGFAVLGLQRLRIGRRELARTLHR"
     gene            653..1978
                     /locus_tag="VIX62_12030"
     CDS             653..1978
                     /locus_tag="VIX62_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861005.1"
                     /translation="MSSVVPPAPARTPLPEGTIPVGIGLLLAGFATYAFFRVGTVAVG
                     GPDEFEPLASLWFVTFALAPGLFLPLEQELGRAISHRRALGQGSQPVIQKVVRLGAVM
                     VGIAVLCTAIAGPFLASSYFDGDWIMVVALALTFVGYAPAHLARGICSGSGRFTEYAI
                     IMGSDGIVRIVLCVVLAIIGITAVGAYGLVIAISPLFAVAYVYRRGGLQSEPGPEADW
                     HEVTPNLGWLLLGSIFAATLLNAGPIAASALAGENEDGLVTAFSYGVLLARIPLFLFQ
                     AVQAALLPRLSRLAARGEIDEFRAGLRRLVVVVIGVGVVGVAGSLLLGSWAIDLVYKE
                     DLSGRTLAMLSFSSACYMMSLAIGQAVIALKGHALVAVGWGIGVATFLLAMWLNGEEV
                     FRRIEIALVLSSLATTIVFAIALQFRLRSGVVPDRASIMEAITDMPFET"
     gene            complement(2063..2935)
                     /locus_tag="VIX62_12035"
     CDS             complement(2063..2935)
                     /locus_tag="VIX62_12035"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY5861006.1"
                     /translation="MAEEFPYVELVSGDLADLPSLVAALEAAQPDEVYNLGAISFVAL
                     SFSQAELTANLTGLGVLRMLEAIRMVGGSQNNPIRFYQASSSEMFGKVRETPQTELTP
                     FHPRSPYGCAKVFGHDIVVNYRESYGLHASSGILFNHEGPRRGMEFVTRKVTNAAARI
                     KLGLQHEVVLGNLDSKRDWGYAGDYVKAMWLMLQQDEPGDFVVATGETHSIEELVERA
                     FGEVGIDNWRDYVRQDPKFLRPAEVDLLIGDPSKARNVLGWEPEVDFPALIKMMVAHD
                     LQLEATKHNLPLPG"
BASE COUNT          410 a         1017 c          988 g          535 t
ORIGIN      
        1 ctcgttggcg accggcccga agcccggatt gccggcgacc ggccggacga acgcgccggg
       61 gaggcgagcc gtgatgcggt cctcgacggc gtcgaacgcc gtgtcggtgt cggcgacccc
      121 gtcaccggcc acgaggaaca ggaatcgcag gttcgggtag tcctggcggg cgaacgcttc
      181 gaccgtgtcc tcgaaccacg cctcggggcg gtgcaccacg acgacagcca ccacgggcgg
      241 tgcctgcgct gccgccatct gcgcttctgc gggcgcgcgc tcggttgcac cgacgtgcac
      301 ggtctccggt tccacgattg atcgaggcta gcggtgctcc gtcgaaccga cgggtcagcc
      361 cgctgcgacg cctgacaccg actcgatagt ctcccgccca tggcgccccg gccgcgattc
      421 gacccgacga gcctcgatct caccgatttc gacgtgtcgg cgatgctcga ttccgacgcc
      481 ggacgcgccg cccgcaccgt cgtgggcgcg gccgccaccg gcgtgcgcga ggtgacctac
      541 gtcacggtcg gattcgccgt gctcggactg caacgactgc gcatcgggcg ccgcgagttg
      601 gccaggactc tgcaccggta gtcggacact gcctcgctag gcttcggcag tcgtgtcgtc
      661 cgtcgtccct cccgcgcctg cccgcacacc gctgccggaa gggaccatcc cggtcggtat
      721 cggcctgctg ctcgccggat ttgccaccta cgccttcttc cgcgtgggca cggttgccgt
      781 cggtggcccg gacgagttcg agccgctcgc ctcgctgtgg ttcgtgacgt tcgccctcgc
      841 gcccggcctc ttcctgccgc tcgaacagga gctcggtcgc gccatctcgc accgccgggc
      901 gctcggccaa gggtcgcagc cggtcatcca gaaggtcgtc cgcctgggag cggtcatggt
      961 ggggatcgcg gtgctctgca cggcgatcgc cggcccgttc ctggcgtcgt cgtacttcga
     1021 cggcgactgg atcatggtgg tcgctctggc gttgaccttc gtcggttatg cccctgccca
     1081 cctggcacgc ggcatctgct cgggcagtgg ccggttcacc gaatacgcga tcatcatggg
     1141 ctccgacggc atcgtccgca tcgtgctctg cgtcgtgttg gcgatcatcg ggatcacggc
     1201 ggtcggcgcc tacggcttgg tgatcgccat ctcgccgctg ttcgccgtgg cctacgtgta
     1261 ccggcggggc ggcctgcaga gcgaacccgg gccggaagcc gactggcacg aggtcacacc
     1321 gaacctgggt tggctgctcc tcggctcgat cttcgccgcg acgttgctca acgccggacc
     1381 gatcgccgcg agtgcactgg ccggtgagaa cgaggacggc ctggtcaccg cgttctcgta
     1441 cggcgtgctg ctcgcccgga tccccctgtt cctgttccag gccgtccagg ctgccctcct
     1501 gccacggctc agtcgcctgg ccgcccgcgg cgagatcgac gagttccgcg ccggcctacg
     1561 ccgactggtc gtcgtggtga tcggtgtcgg cgtcgtcggc gtcgccgggt cgttgctgct
     1621 cggctcgtgg gcgatcgacc tggtgtacaa ggaggacctg agcgggcgga cgttggcgat
     1681 gctgtcgttc agcagcgcct gctacatgat gtcgctcgcc atcggtcagg cggtcatcgc
     1741 cctgaagggc catgcgctcg tcgccgtcgg ctggggcatc ggtgtggcga cgttcctgct
     1801 cgccatgtgg ctcaacggcg aagaggtgtt ccgtcgcatc gagatcgcac tcgtgttgtc
     1861 gtcgctggcg accacgatcg tcttcgcgat cgccctccag ttccggctgc gttcgggcgt
     1921 cgtccccgac cgcgcctcga tcatggaagc gatcaccgac atgcccttcg agacctgacc
     1981 cgggaatgcg agttcggttc gttttggccc cgtccccaaa cgaaccgggt tcgtttgggg
     2041 acggggccag aacgaaccga ggtcagccgg ggagggggag gttgtgtttg gtggcttcga
     2101 gctggaggtc gtgggcgacc atcatcttga tcaacgccgg gaaatcgacc tcgggctccc
     2161 agcccagcac gttgcgggcc ttggacggat cgccgatcag caggtcgact tcggcggggc
     2221 gcaggaactt ggggtcctgg cggacgtagt cacgccagtt gtcgatcccc acctcaccga
     2281 acgcgcgctc caccaactcc tcgatcgaat gcgtctcacc cgtggccacg acgaagtcgc
     2341 ccggttcgtc ctgctgcaac atcaaccaca tcgccttgac gtagtcgccc gcataccccc
     2401 agtcgcgctt ggagtcgagg ttgccgagca ccacttcgtg ctggagaccc agcttgatgc
     2461 gggcggcggc gttggtcacc ttgcgggtca cgaactccat cccccgccgc ggtccctcgt
     2521 ggttgaacaa gatcccggag gaggcatgga ggccgtagga ctcgcggtag ttcacgacga
     2581 tgtcgtgccc gaacaccttg gcgcacccgt acgggctgcg cgggtggaac ggggtcagtt
     2641 cggtctgcgg ggtctcacgg accttgccga acatctccga actggaggcc tggtagaagc
     2701 ggatcgggtt gttctggctg ccgccgacca tgcggatcgc ctccaacatc cgcaacaccc
     2761 cgagaccggt caggttcgcc gtcaactccg cctgcgagaa gctcaacgcc acgaagctga
     2821 tcgctcccag gttgtacacc tcatcgggct gcgccgcctc gagcgcggcg accagtgacg
     2881 gcaggtcagc caggtcaccg gagacgagtt ccacgtaggg gaactcctcg gcgatctgct
     2941 gggcccgggg
//
LOCUS       DATDBZ010000518         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2219898, whole genome shotgun sequence.
ACCESSION   DATDBZ010000518 DATDBZ010000000
VERSION     DATDBZ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2219898"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1159)
                     /locus_tag="VIX62_12040"
     CDS             complement(20..1159)
                     /locus_tag="VIX62_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5861007.1"
                     /translation="MSQISDAFRGIQLGNRWDRAPKWVRSVGLLLFIAFAFYLPFLNI
                     LPFAYIRTDLNPTGSDWSSVLFLIVVYMIAAVGLNVVIGFAGLLDLGYVGFYALGAYS
                     VALFGSPSSPVVEAMQSRFGLSEEWAVPFVMCIPIAIAMALTAGVILGAPTLRLRGDY
                     LAIVTLGFGEIIRITARNLDPITGGAAGITQVPVPPGPEIDGRSFFNTIDAERWYWLA
                     LVILILIVILASRLEHSRVGRAWLAIREDEDAAAVMGVAGFKFKLWAFAIGASVGGLA
                     GLLFGSKQQYVEPNAFMLNLSFLFVAMVVIGGSGNIVGVLVGAFLLTYLPERFRELQE
                     WRPFAFGLALVLVMVLRPQGLVPSRRRAAEIEDRRHEAEEAAADA"
     gene            complement(1156..2127)
                     /locus_tag="VIX62_12045"
     CDS             complement(1156..2127)
                     /locus_tag="VIX62_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018817381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5861008.1"
                     /translation="MDFDALFSGLDRHTLDGITRGSIYALVALGYTMVYGVMKLINFA
                     HSEVFMVGTWTVLGVYTVLGATSGMGVGAVIGATVLALLAAMLASAGTALAVERIAYR
                     PLRRKGAPPLIFLITGIGCSLVLVETFGQVLKAFFGPPFGRVTVNVPSVIETKVVFTI
                     PGLDLPVTNTRLLIILGAIVMMVALDTFVNKSRLGRGVRAVAQDPDTASLMGVNQERV
                     IMLVFVLGGAMAGVAALLFTIQYDITKFDIGFIIGIKAFTAAVLGGIGNLRGALVGGL
                     LLGVVEVYASQIWDSSWTDVTAFIVLVLVLMFRPTGLLGESLGKARA"
BASE COUNT          418 a          693 c          785 g          287 t
ORIGIN      
        1 ggcgccctcg atctcgtgct caggcatctg cggcagcctc ctcggcctcg tgtcgtcgat
       61 cttcgatctc tgcggctcgt cgtctgctcg gcaccaggcc ctggggtcgg aggaccatca
      121 ccagcaccag cgccaggccg aaggcgaagg ggcgccactc ctggagctca cggaaccgct
      181 cggggaggta ggtgagcagg aaggcaccga cgaggacgcc gacgatgttg cccgaacccc
      241 cgatgaccac catggccacg aagaggaacg acaggttcag catgaaggcg ttgggctcga
      301 cgtactgctg cttgctgccg aacagcagcc cggcgagccc gccgacggac gcgccgatcg
      361 cgaaggccca cagcttgaac ttgaagcccg cgacgcccat caccgccgcc gcgtcctcgt
      421 cctcgcggat ggccagccag gcacgaccga cgcggctgtg ctcgaggcga ctggccagaa
      481 tgacgatgag gatgaggatg acgagcgcca gccagtacca gcgctcggcg tcgatggtgt
      541 tgaagaacga ccgcccgtcg atctccggcc cgggcggcac ggggacctgg gtgatgcccg
      601 cggcaccgcc ggtgatgggg tcgaggttgc gcgcggtgat gcggatgatc tcgccgaacc
      661 cgagggtgac gatggcgagg tagtcgccgc gcagccgcag cgtcggagcg ccgaggatca
      721 cacccgccgt gagcgccatc gcgatcgcga tggggatgca catcacgaac gggacggccc
      781 attcctcgga caggccgaaa cgcgactgca tggcctccac gaccggcgag gacggcgacc
      841 cgaagagggc caccgaatag gcgcccaggg cgtagaaccc gacgtagccg aggtcgagca
      901 gtccggcgaa gccgatcacc acgttgaggc cgacggccgc gatcatgtag acgacgatga
      961 ggaacagcac gctcgaccag tcggagccgg tcgggttcag gtcggtgcgg atgtaggcga
     1021 acggcaggat gttgaggaac ggcaggtaga acgcgaaggc gatgaacagc agcaggccga
     1081 cggagcggac ccacttcggg gcacggtccc agcggttgcc gagctggatg ccgcggaagg
     1141 cgtcggagat ctggctcatg cgcgtgcctt tccgagggac tcaccgagca gtccggtggg
     1201 ccggaacatc aggaccagga cgaggacgat gaacgcggtg acgtcggtcc agctggagtc
     1261 ccagatctgg gaggcgtaca cctcgaccac gcccagcagc agaccgccga ccagcgcacc
     1321 gcgcaggttg ccgatgccgc cgaggaccgc ggcggtgaac gccttgatgc cgatgatgaa
     1381 gccgatgtcg aacttcgtga tgtcgtactg gatggtgaac agcagggcgg cgacgccggc
     1441 catcgcgcca ccgagcacga agacgagcat gatcacgcgc tcctggttga cgcccatcag
     1501 ggaggcggtg tccgggtcct gtgccacggc gcggacgccg cggccgagcc ggctcttgtt
     1561 cacgaacgtg tccagcgcga ccatcatgac gatggcgccg aggatgatca gcagccgggt
     1621 gttggtgacg ggcaggtcga ggccggggat cgtgaagacg accttggtct cgatcacgga
     1681 cggcacgttg accgtcacgc gaccgaaggg cgggccgaag aacgctttca gcacctggcc
     1741 gaaggtctcg acgagcacga gcgagcagcc gatgccggtg atcaggaaga tcagcggcgg
     1801 agcacccttc ctgcgcagcg gccggtaggc gatccgttcg acggccagtg cggtgcctgc
     1861 cgaggcgagc atcgcggcga gcagtgccag cacggtggcg ccgatgaccg cgccgacccc
     1921 catgccggac gtggcgccga ggacggtgta gacgccgagc accgtccagg tgcccaccat
     1981 gaacacctcg gagtgggcga agttgatcag cttcatcacg ccgtacacca tggtgtaacc
     2041 gagcgcgacg agcgcgtaga tggatccgcg ggtgatgccg tccaacgtgt gacggtccaa
     2101 cccgctgaac agggcgtcga agtccactca gggccctccc agtagtgcgg cccggtccgg
     2161 gccatggagc gggggcgggc gag
//
LOCUS       DATDBZ010000519         2331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_376274, whole genome shotgun sequence.
ACCESSION   DATDBZ010000519 DATDBZ010000000
VERSION     DATDBZ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376274"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VIX62_12050"
     CDS             <1..192
                     /locus_tag="VIX62_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY5861009.1"
                     /translation="QHTHHLLCPCHQSQFDVANHCEVIFGPAKRPLPQLPIAVDDEGY
                     LIAQSDFTEPVGPSFWERN"
     gene            192..1796
                     /locus_tag="VIX62_12055"
     CDS             192..1796
                     /locus_tag="VIX62_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEY5861010.1"
                     /translation="MIAPSTNVPQKRSFTASASVYVNDRTSLAGFVKELGRKAFPDHW
                     SFLLGEIALFSFVVILISGTFLTFFFQASMAEVHYDGSYVPLKGVEMSVAMASTLDIS
                     FDIRGGLFVRQMHHWAALLFVAAIGLHMLRIFFTGAFRKPREFNWIIGFTLFILAMGE
                     GFTGYSLPDDLLSGNGLRIIDGLIKGIPVIGTWTSFLLFGGEFPGTAIVGRLYSLHIL
                     LLPAIIVALIAIHLVLVVVHKHTQYAGPGKTQQNAVGPPILPIYAAKAGGFFFIIFGV
                     VALIASLFTINPIWNYGPYDPSPVSAGTQPDWYIGFADGALRLIPPGWEFVWLDRTWS
                     FNILVPLIAIGIFILLVVAYPFIEAWVKGDKREHHIADRPRNAPTRTAIGAAGVTFYA
                     GLWAAASSDILATHFSLTMEGVIHALQAMVVLGPFVAYFIAKRVCIALQKKDREIVLH
                     GYESGRIVKLPGGEFIEVHQPLDEYDRWRLVSFETYEPLMIRPNSRGKITVGQRMRAG
                     MSRWFFEDRIAPVTKGELEATHSNHH"
     gene            1829..>2331
                     /locus_tag="VIX62_12060"
     CDS             1829..>2331
                     /locus_tag="VIX62_12060"
                     /inference="COORDINATES: protein motif:HMM:NF045339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5861011.1"
                     /translation="MSIDTTWLRCPNCLLDLSAIDDLVFGCPNGHRFDRARQGYLTLL
                     PPRAPHTIGDDREMLAARVALLDHGAYSPIAEAVAAAAAAVPAMAPGAPARIADLGCG
                     TGYYSAAVARTLPAATFLLADRSPDAVRISLRALPHATGVVLDLWRPLPIRDDTVDVA
                     LNVFAPR"
BASE COUNT          345 a          868 c          699 g          419 t
ORIGIN      
        1 cagcacacgc atcacctgct ctgcccctgc caccagtcgc agttcgacgt ggcgaaccac
       61 tgcgaggtca tcttcggccc ggccaagcgg cccctgccgc agctgccgat cgcggtcgac
      121 gacgagggct acctcatcgc gcagagcgac ttcaccgaac ccgtcggccc gagtttctgg
      181 gagcgaaatt gataatcgca ccgtccacca acgttccgca gaagcggtcg ttcacggcat
      241 ccgcgtcggt ctacgtcaac gatcgcacca gcctcgccgg cttcgtcaag gagctcggac
      301 gcaaggcgtt ccccgaccac tggtccttcc tcctcggcga gatcgcgctc ttcagcttcg
      361 tcgtcatcct gatctcgggc acgttcctca ccttcttctt ccaggcctcg atggcagagg
      421 tgcactacga cggctcctac gtgcccctca agggcgtcga gatgtcggtc gcgatggcgt
      481 cgacgctcga catctcgttc gacatccgcg gcgggctctt cgtgcgccag atgcaccact
      541 gggccgcgct gctgttcgtc gccgccatcg gcctgcacat gctgcgcatc ttcttcacgg
      601 gagccttccg caagcctcgc gagttcaact ggatcatcgg gttcaccctc ttcatcctcg
      661 cgatgggcga gggcttcacc ggatactcgc tccccgacga cctgctctcg ggcaacggcc
      721 tccgcatcat cgacggcctg atcaagggca tccccgtgat cggcacctgg acctcgttcc
      781 tgctgttcgg cggcgagttc ccgggcaccg cgatcgtggg acgcctgtac tcgctgcaca
      841 tcctgctgct gcctgccatc atcgtggcgc tcatcgcgat ccacctggtg ctcgtcgtcg
      901 tccacaagca cacccagtac gccggaccgg gcaagaccca gcagaacgcc gtcggcccgc
      961 ccatcctccc gatctacgcg gcgaaggccg gcggcttctt cttcatcatc ttcggtgtcg
     1021 tggccctgat cgcctcgctg ttcacgatca acccgatctg gaactacgga ccgtacgacc
     1081 cctcgcccgt gtcggcgggt acgcagccgg actggtacat cggcttcgcc gacggcgcgc
     1141 tgcgcctgat cccgccgggc tgggagttcg tctggctcga ccgcacgtgg tcgttcaaca
     1201 tcctcgtccc gctcatcgcg atcggcatct tcatcctcct cgtggtcgcg tacccgttca
     1261 tcgaggcgtg ggtgaagggc gacaagcgcg agcaccacat cgccgaccgt ccgcgcaacg
     1321 cgcccacccg caccgcgatc ggtgccgccg gtgtcacgtt ctacgccggc ctctgggcgg
     1381 cagcgagctc cgacatcctg gcgacgcact tctcgctcac gatggagggc gtcatccatg
     1441 ccctgcaggc catggtggtc ctcggcccgt tcgtcgccta cttcatcgcc aagcgcgtgt
     1501 gcatcgcgct ccagaagaag gaccgcgaga tcgtgctgca cggctacgag tcgggtcgca
     1561 tcgtgaagct ccccggcggc gagttcatcg aggtgcacca gccgctcgac gagtacgacc
     1621 gttggcgcct cgtgagcttc gagacgtacg agccgctgat gatccgtccg aactcgcgtg
     1681 gcaagatcac cgtcgggcag cgcatgcgcg cggggatgtc ccggtggttc ttcgaggacc
     1741 gcatcgcgcc ggtcaccaag ggcgagctcg aggcgacgca ctccaaccac cactgagccg
     1801 accgctcacg gattcgggca ccggtcccat gtcgatcgac acgacatggc tccggtgccc
     1861 gaactgtctg ctggacctct cggcgatcga tgatctcgtc ttcggctgcc cgaacggaca
     1921 ccgcttcgac cgcgccaggc agggctacct cacgctgctg ccgccgcgcg cgccgcacac
     1981 gatcggcgac gatcgcgaga tgctggcggc tcgtgtcgcg ttgctcgacc atggcgccta
     2041 ctcccccatt gccgaggccg tcgccgcggc cgcagccgcg gttccggcga tggcgccggg
     2101 tgcaccggcg cggatcgccg acctggggtg cggaacgggc tactactcgg cggcggtcgc
     2161 tcgcacactc cccgccgcga cgttcctcct cgccgaccgg tcacccgatg cggtccgcat
     2221 cagcctccgc gcgctccccc atgcgacggg ggtggtgctc gacctgtggc ggccgctgcc
     2281 gatccgcgac gacacggtcg acgtcgccct caacgtcttc gcgccgagga a
//
LOCUS       DATDBZ010000520         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2407163, whole genome shotgun sequence.
ACCESSION   DATDBZ010000520 DATDBZ010000000
VERSION     DATDBZ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2407163"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="VIX62_12065"
     CDS             complement(<1..1662)
                     /locus_tag="VIX62_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5861012.1"
                     /translation="MKNKAWIVYPVAAVAATVAYYLLGHNSYLFNLIGLSSPVLILVA
                     VHLHKPERRAPWFLIAAGQFLFIAGDVVSYNYERFAESLPQLFPLDYTFNPAGDVPFP
                     GLADVLYLSVYPCLIAGVVMLIQARNPGRDRASLLDSAMVAMGVGTVTWVLLIAPYLD
                     IADLDLKIKLTAMAYPVMDLLLAAAAIRLAVGAGRRPVAFYLLMASVVALFVTDAVYA
                     WFGLYTESGYQPGSGWLEAGWMSFYVLLGTAALHPSMRVLTERAPETEERLTFGRLVL
                     LTIAALSPPVIRLVQMARGEEVDVGVLSAASIVLFLLVVLRMAGLIRQQERSAERERA
                     LRKAGATLVTATSREGIHEAAIDAARALAGQEAAIRMCEEPDGGDEIVVVAAAGGSVD
                     VHGAHFSFSTLQEWKQQRLLDNDAYAISAYESTLRGPLALPFGDDGSVFVAPLFIKEE
                     LHGLMVVATPEEMPRSVADSLRALSSQVALALESATLTEDLLIKQSEARFASLVSNSS
                     DVVCVLDPDTTVSYASPSAARVLGFDPAQIEGTRFADLIHPDDMTR"
     gene            complement(1714..>2071)
                     /locus_tag="VIX62_12070"
     CDS             complement(1714..>2071)
                     /locus_tag="VIX62_12070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5861013.1"
                     /translation="KAPLYEMARHAAMKLGSRGIRVNTVSPGTVLTPMQPADDPEARI
                     SRVATCLGRPAFPDEVAGVYVFLASDDSAYVTATDIRADGGWLDGLTEAHAEALLAGI
                     DDTTIPRVGNTAGQSP"
BASE COUNT          348 a          667 c          735 g          321 t
ORIGIN      
        1 gcgagtcatg tcgtcggggt ggatcagatc ggcgaaccgc gtgccctcga tctgcgccgg
       61 gtcgaagccg agcacacgtg ccgcggatgg gctcgcgtag ctcacggtcg tgtcgggatc
      121 gagcacgcac accacgtcgg atgagttgct gacgagcgag gcgaacctcg cttcgctctg
      181 cttgatcagc aggtcctcgg tgagggtggc ggactcgagc gccagggcga cctgcgacga
      241 gagggcgcgc agcgagtcgg cgaccgaacg cggcatctcc tcgggggtcg ccacgaccat
      301 caggccgtgc agttcctcct tgatgaacag cggggccacg aagaccgatc cgtcgtcgcc
      361 gaagggcagg gcaagaggcc cccgcaacgt gctctcgtag gcgctgatcg cgtaggcgtc
      421 gttgtcgagc aggcgctgtt gcttccattc ctgcaaggtc gagaacgaga agtgcgcgcc
      481 gtgcacgtcg accgatccgc cggccgcggc caccaccacg atctcgtcac ccccatcggg
      541 ttcctcgcac atgcggatcg cggcctcctg gcctgcgagg gcacgggcgg cgtcgatcgc
      601 cgcttcgtgg atcccctccc ggctcgtcgc cgtgacgagg gtggcgccgg ccttccgcag
      661 ggctcgttct cgctcggccg atcgctcttg ctgccggatc aacccggcca tgcgcaacac
      721 gaccaatagg aagagcacga tggacgcggc gctcagcacg ccgacgtcga cctcttcccc
      781 gcgggccatc tgcacgaggc ggatcaccgg gggggagagg gccgcgatcg tgagcaggac
      841 gagtcgcccg aatgtgaggc gctcttcggt ctcgggtgcg cgttcggtca gcacccgcat
      901 cgagggatgc agggcagcgg tcccgagcag cacgtagaac gacatccatc cggcctcgag
      961 ccagccgctg cccggctggt agccgctctc ggtgtagagg ccgaaccacg cgtagacggc
     1021 gtcggtgacg aagagcgcca cgaccgaggc catcagcagg tagaaggcga cgggccggcg
     1081 gcccgcgccg accgccaagc ggatcgcggc ggcggcgagc agcaggtcca tgacggggta
     1141 ggccatggcc gtgagcttga tcttcagatc gaggtcggcg atgtcgaggt acggcgcgat
     1201 cagcagcacc catgtcacgg tgcccacgcc catcgccacc atcgcggagt cgagcaggct
     1261 cgcccggtcg cgcccagggt tccgtgcctg gatcaacatc acgacgccgg cgatcaggca
     1321 tgggtagacg ctcaggtaca acacgtcggc gagccccggg aacggcacgt cgccggcggg
     1381 gttgaaggtg tagtcgagcg ggaagagctg tgggagcgat tcggcgaatc gctcgtagtt
     1441 gtaggagacc acgtcgccgg cgatgaacag gaactggccg gccgcgatga ggaaccaggg
     1501 cgcgcgtcgt tccggcttgt gcaggtgcac ggccacgagg atcagcaccg gcgaggacaa
     1561 gccgatcagg ttgaacaggt agctgttgtg acccagcagg taataggcga cggtcgcagc
     1621 gaccgcagcc acgggataca cgatccaggc cttgttcttc acctgtgcgt tatcggtcgg
     1681 cgtcctcacg ggctgatacc tgattccggg tagtcacggc gactgacctg cggtgttgcc
     1741 gactcgggga atggtcgtgt cgtcgatgcc ggcgagcagc gcctcggcgt gtgcctcggt
     1801 gagcccgtcc aaccagccac cgtcggctcg gatgtcggtc gcggtcacgt aggcgctgtc
     1861 gtcggatgcc aggaagacgt acacgccggc gacctcgtcg gggaacgccg gtcgaccgag
     1921 gcacgtcgcg accctggaga tacgtgcctc tggatcatcg gctggctgca tcggcgtgag
     1981 cacggtgccg gggctcaccg tgttcaccct gatgccgcgc gagccgagct tcatcgccgc
     2041 gtgacgagcc atctcgtaga gcggtgcctt g
//
LOCUS       DATDBZ010000521         2232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_3033938, whole genome shotgun sequence.
ACCESSION   DATDBZ010000521 DATDBZ010000000
VERSION     DATDBZ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3033938"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VIX62_12075"
     CDS             <1..1043
                     /locus_tag="VIX62_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861014.1"
                     /translation="SVIVGTSLVVAAVASAVKTVVIPRQASSIITRGVFAGLHRAYDL
                     VTPASMSYERRDRVLATFAPLGMLGLLVTWISLTFLGYAGIFWGLDGGTSSTVALELS
                     GSSLLTLGFLRPSTFWLMMVSFTEAAIGLFLLALLITYLPTINGAFSRREQGVTALAV
                     RAGEPPSGVEMVLRYWRLERMQELNAVWEEWERWFVDVEETHTSFSALVFFRAPQADH
                     SWVTAAGAVLDGAALTISTIEVPHDVRADICIRAGYLSLRRIAEGYGISYERQPAPGD
                     PISITRQEWDDAIDDLAASGVPIKADRDQAWLDFAGWRVNYDTVLLELADLTDAPYAM
                     WSSDRGVLRPDR"
     gene            complement(1051..1530)
                     /locus_tag="VIX62_12080"
     CDS             complement(1051..1530)
                     /locus_tag="VIX62_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861015.1"
                     /translation="MELLAFNTTLYKAIVFVHIFAAIIWVGGGFFFQVKIAQFKRAND
                     NAGFLQLGKDAEHFGMKLFMPMSVVVLLAGITMVWYGPYTFQLWIALALFGIVATALT
                     GALYLGPTGGKLAKLAEEKGFDDPSVTAKRDQLVLVSRLDYAVLALIVLDMVFKPGA"
     gene            complement(1605..>2232)
                     /locus_tag="VIX62_12085"
     CDS             complement(1605..>2232)
                     /locus_tag="VIX62_12085"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5861016.1"
                     /translation="EQNEVSWEAIKDGFRFLKGKRTIQSVFLADLNAMVFGFPIALFP
                     AVAERLGGGEVALGLLYAAPAAGAFLATMLSGRARHVRRQGRAILVAVAVWGSAIVVF
                     GFSEALWLSLAMLAVAGAGDMVSGIYRATILQTAVEDRLRGRLDGIGMAVWATGPSLG
                     NVESGVVASLTTVPFSIVSGGLLTIAGVGLLRWFAPGFWRYDARDPSP"
BASE COUNT          359 a          803 c          735 g          335 t
ORIGIN      
        1 tctcggtgat cgtgggtacg agcctcgtcg tcgccgcggt ggcatcggcg gtcaagacgg
       61 tggtgatccc ccgccaggcg agctcgatca tcacgcgcgg ggtgttcgcc gggctgcacc
      121 gggcatacga cctcgtgacc ccggcctcga tgtcgtacga acgccgtgac cgcgtgctcg
      181 cgaccttcgc gccgctcggc atgctcggcc tgctcgtcac ctggatcagc ctcacgttcc
      241 tcgggtacgc cggcatcttc tggggcctcg acggtggcac gagctccacg gtcgccctcg
      301 agctgagcgg gtcgtcgctg ctcacgctcg gattcctgcg accgtcgacc ttctggctga
      361 tgatggtgtc gttcaccgag gcggcgatcg gcctgttcct gctggcgctg ctgatcacct
      421 acctgcccac gatcaacggc gcgttctcgc ggcgggagca gggcgtcacc gcgctcgcgg
      481 tgcgcgccgg cgagcccccc tcgggcgtgg agatggtgct gcggtactgg cgcctcgaac
      541 gcatgcagga gctgaacgcg gtatgggagg agtgggagcg ctggttcgtc gacgtcgagg
      601 agacccacac gtcgttctcg gcgctcgtgt tcttccgcgc cccgcaggcc gatcactcgt
      661 gggtgaccgc ggccggcgcc gtgctcgacg gcgccgcact cacgatctcg accatcgagg
      721 tgccccacga cgtgcgcgcc gacatctgca tccgcgccgg ctacctgtcg ctgcggcgca
      781 tcgccgaagg ctatggcatc agctacgagc gccagccagc ccccggcgac ccgatctcga
      841 tcacgcggca ggagtgggac gacgcgatcg acgacctcgc ggcgtcgggc gtgccgatca
      901 aggccgatcg cgaccaggcg tggctcgact tcgccggctg gcgcgtgaac tacgacaccg
      961 tgctgctgga gctcgccgac ctcaccgacg ccccgtacgc gatgtggtca tcggaccgcg
     1021 gggtgctgcg gccggaccgc tgaggccacc tcacgcgccc ggcttgaaca ccatgtcgag
     1081 cacgatgagt gcgagcacgg cgtagtcgag gcgcgacacc aagaccaatt ggtctcgctt
     1141 ggctgtcacg ctcggatcgt cgaacccctt ctcctcggcg agcttcgcga gcttgccgcc
     1201 ggtcggcccg aggtagagcg ccccggtgag tgcggtcgcg acgatgccga acaacgccag
     1261 cgcgatccag agctggaacg tgtatgggcc gtaccagacc atcgtgatgc cggcgagcag
     1321 cacgacgacc gacatcggca tgaacagctt catgccgaaa tgctccgcgt ccttgccgag
     1381 ctgcaggaac ccggcgttgt cgttggcccg cttgaactgg gcgatcttca cctggaagaa
     1441 gaatccgccg cccacccaga tgatcgccgc gaagatatgg acgaagacga tcgccttgta
     1501 gagcgtcgtg ttgaacgcga gcagttccac cgtcgagcac ccctcccgtg tgtgtcggtg
     1561 cgccgcgcac gatacccccg ccgtggggct gggggaagca ccgatcacgg cgaagggtcg
     1621 cgagcgtcgt agcgccagaa gcccggcgcg aaccaccgca gcaaccccac gccggcgatc
     1681 gtgagcagcc cgcccgacac gatcgagaac ggcaccgtgg tgagcgaggc gacgaccccc
     1741 gattccacgt tgcccagtga gggccccgtg gcccacaccg ccatgccgat gccgtcgaga
     1801 cgcccgcgca ggcggtcttc gaccgcggtc tgcaggatcg tggcgcggta gataccgctc
     1861 accatgtctc ccgcaccggc gacggcgagc atcgcgagcg acagccagag cgcttcggag
     1921 aacccgaaca cgacgatcgc cgaaccccac acggcgacgg cgacgaggat cgcccggccc
     1981 tgccggcgca cgtgcctcgc ccgccccgag agcatcgtcg cgaggaacgc gccagccgcc
     2041 ggcgccgcgt acagcagccc gagcgccacc tcgccgccgc ccagtcgctc cgccaccgcc
     2101 gggaacagcg cgatcgggaa cccgaagacc atggcgttca ggtcggccag gaacaccgac
     2161 tggatcgtgc gcttgccctt caagaagcgg aacccgtcct taatcgcctc ccagctcacc
     2221 tcgttctgct cg
//
LOCUS       DATDBZ010000522         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2224316, whole genome shotgun sequence.
ACCESSION   DATDBZ010000522 DATDBZ010000000
VERSION     DATDBZ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224316"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VIX62_12090"
     CDS             complement(<1..552)
                     /locus_tag="VIX62_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5861017.1"
                     /translation="MPVERVFAMPDLGEGLEEGEITAWLIAEGDTVALNQPIVEVETA
                     KATVEIPSPFAGVVARLHGAVGDAIPVGQPLVTFDVAGDETVDDVAPSPRAAGRVAAS
                     PAVRKLARELSIDLDTVVGTGPQGRVTADDVHAAAGPTPAPDRDPSTDVVPLTPTRRA
                     IAENIARQASIPQVTTFRTVDCTA"
     gene            complement(552..1526)
                     /locus_tag="VIX62_12095"
     CDS             complement(552..1526)
                     /locus_tag="VIX62_12095"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY5861018.1"
                     /translation="MTEFTMSGALNAALRDSLRDDPRMLVFGEDVGRSGGVFRVTDGL
                     QAEFGVERVFDTPISEAGIAGAAVGLAFAGWRSVIEMQFDSFSYPALEQIVDHIAKMR
                     ERTRGVVSMPITIRIPSFGGIRGKEHHGESPETFYVHTAGLKVLAPSGALDAYRLLRH
                     AIADPDPVVVLEPKARYWAKEAGELTTEGPGIGVGRVVREGDGCAVFAYGAMVARTLE
                     AADAVEAEGIAAKVIDLRTLSPLDVDLIAASVSETGRAVVVHEAPRTLGLGAEVTALI
                     VEHAFDFLEAPVARVTGWDVPYPPAALEQFYLPSVERIADAIRTTVAY"
     gene            complement(1519..>2012)
                     /locus_tag="VIX62_12100"
     CDS             complement(1519..>2012)
                     /locus_tag="VIX62_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014178027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5861019.1"
                     /translation="NNGWAISMPTAQQTAGEIWKRAEGYGFPGVRVDGNDVLAVYRAT
                     KEAADRARAGGGPTLIEALTYRIGAHSTADDPSRYRDDTEVEVARAFDPIARFRTWLT
                     AGGTVDDATVESWEQEIDAEVLAIREGVVAQAAPPVEAMFDHAYAAPTAQFERMRGEA
                     LSD"
BASE COUNT          302 a          723 c          661 g          326 t
ORIGIN      
        1 cgctgtgcaa tcgaccgtcc ggaaggtggt gacctgcggg atcgaagcct gccgcgcgat
       61 gttctcggcg atcgcgcggc gcgtcggtgt gagcggcacg acgtcggtgc tcgggtcgcg
      121 gtcaggcgcg ggcgtgggcc cggccgcggc atgaacgtcg tccgcggtca cgcgaccctg
      181 cggccccgtc ccgaccacgg tatccaggtc gatcgagagt tcacgggcca gcttgcggac
      241 cgcgggactc gcggcgacac gccctgcggc acgaggcgag ggcgcaacgt catcgaccgt
      301 ctcgtcaccc gccacatcga aagtcaccaa cggttgcccc acggggatcg cgtcgccgac
      361 ggccccatga agccgcgcca ccacgcccgc gaacggcgag gggatctcga cggtggcctt
      421 cgcggtctcc acctcgacga tcggttggtt cagggccacc gtgtcgcctt ccgcgatcag
      481 ccatgcggtg atctcaccct cctccaaacc ctcgcccagg tccggcatcg cgaacactcg
      541 ctcgaccggc atcagtaggc caccgtggtc cggatcgcgt ccgcgatgcg ttcgacgcta
      601 ggcaggtaga actgctctag tgccgcaggg gggtagggca catcccaccc ggtcactcga
      661 gcgacgggag cctcgaggaa gtcgaacgcg tgctcgacga tcaacgccgt cacttcggcg
      721 ccgagcccca gggtccgcgg cgcttcgtgc acgacgactg ctcggcccgt ctcgctgacg
      781 ctcgcggcga tgaggtcgac gtccagcggc gagagcgttc ggagatcgat gaccttcgcg
      841 gcgatgcctt cggcctccac ggcatcggcg gcttccagcg tgcgtgccac catggcgccg
      901 taggcgaaca ccgcgcaccc gtcaccctct cggacgactc gcccgacacc gatacccggg
      961 ccctcggtcg tgagttcgcc ggcttccttc gcccaatagc gggccttcgg ttccaggacg
     1021 accacagggt cggggtcggc gatcgcgtgg cgcagaaggc gataggcgtc caaggcgccg
     1081 gatggggcga gcaccttcag cccggcggtg tgcacgtaga acgtctcggg gctctcgccg
     1141 tggtgctcct tgccgcggat gccaccgaac gaagggatgc ggatcgtgat cggcatgctc
     1201 accacgcctc gggtgcgctc ccgcatcttg gcgatgtgat cgacgatctg ttccaacgcg
     1261 gggtagctga acgagtcgaa ctgcatctcg atcacgctgc gccacccagc gaacgccagc
     1321 cccaccgccg cgccggcgat gcctgcctcg ctgatcgggg tgtcgaatac ccgctcgacc
     1381 ccgaattcgg cctgcagccc atccgtgacc cggaacacgc cacccgagcg ccccacgtcc
     1441 tcaccgaaga ccagcatccg tgggtcgtcc cgtagggagt cacgcaaggc tgcgttgagt
     1501 gcgccggaca tcgtgaactc agtcactcag cgcctccccg cgcatgcgct cgaactgcgc
     1561 ggtcggtgct gcgtacgcgt ggtcgaacat cgcttcgacc ggaggggctg cctgcgcgac
     1621 tacgccttcg cggatcgcca ggacttcggc gtcgatctcc tgttcccatg actcgactgt
     1681 ggcgtcgtcg acggtgccgc cggcggtgag ccacgtccgg aaccgcgcga tgggatcgaa
     1741 cgctcgagcc acctcgactt cggtgtcgtc gcgatagcgg ctcggatcat cggccgtcga
     1801 atgcgccccg atgcgatagg tgagtgcctc gatcaaggtg ggaccgccgc ccgctctggc
     1861 ccggtcggcg gcctccttcg tggctcggta caccgccagc acgtcgttgc cgtccacgcg
     1921 gacgcccggg aacccatagc cttccgcacg cttccagatc tcgcccgccg tctgctgggc
     1981 ggtcggcatg ctgatggccc agccgttgtt ct
//
LOCUS       DATDBZ010000523         3945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2002485, whole genome shotgun sequence.
ACCESSION   DATDBZ010000523 DATDBZ010000000
VERSION     DATDBZ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002485"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VIX62_12105"
     CDS             <1..252
                     /locus_tag="VIX62_12105"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEY5861020.1"
                     /translation="LAAVGTLYGGLTAGAKGRETLLPLLLLPVVAPVLIGATRACEAA
                     FGTGGAAVSEGWPWFGLLVVFAATFGAVGTVAFGPLLDE"
     gene            323..1105
                     /gene="ccsA"
                     /locus_tag="VIX62_12110"
     CDS             323..1105
                     /gene="ccsA"
                     /locus_tag="VIX62_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY5861021.1"
                     /translation="MASTADQLSSTRKETSPARRSSPTGTRATRLLGLAVIAGLLWLL
                     VFGLVLSPGDVVQQDSVRIMYVHVPSAWLAYLAFIVTAIASAAYLWRRTRSLAWDRIA
                     GASAEIGVLFMAITLVTGSLWGSVTWGTYWVWDARLTTTAFLFVTYVGYLAVRRLGGS
                     HEARARRSAVLALLAVLEIPLVHFSVVLWRPLHQEASVANPTGDVEMDGLMLFSLFVG
                     IIAFTLLYVWLLLHQQRVLAMEDAIDDHGLDVALAERVKEGM"
     gene            1102..1236
                     /locus_tag="VIX62_12115"
     CDS             1102..1236
                     /locus_tag="VIX62_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861022.1"
                     /translation="MNDAGVIIGSYAFTIVAIGGYVAWMLRRARRLSVLVRDEDRPWT
                     "
     gene            1227..1724
                     /locus_tag="VIX62_12120"
     CDS             1227..1724
                     /locus_tag="VIX62_12120"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY5861023.1"
                     /translation="MDLSPRTTTALDEPSPPARRSKRWGPYVVLGIVLIGGAVVVSKF
                     LTSAIDYYCNADEVGVSAKCSGDRRLRVQGKVEEGSLVEGTNGSVESFVVSFNGAEVP
                     VDYSSGQSLPDLFQECIPVVVAGRLRDGRFEGTSVEVKHSNEYEEDNADRIDQAESAA
                     CLQQA"
     gene            1706..3709
                     /locus_tag="VIX62_12125"
     CDS             1706..3709
                     /locus_tag="VIX62_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY5861024.1"
                     /translation="MLAASLNGSIGVAALLVGLTASVFGAVMLVFGIRRGDARVVASG
                     HRYAWVVAFTGVLAFAAMERALITRDFSLKYVQEVGSSATPALYNFTALWSALEGSLL
                     LWILVLGGYTAAIALRYRARRTDPLVAWAMVVLLVICAFFYSLTLFASNPFASAATLV
                     EDGRGPNQLLQNHVLVLFHPPMLYLGYVGMSVPFAFAIAALVTGRVGEGWLLETRRWT
                     LFAWGFLTAGIILGGWWSYEVLGWGGVWGWDPVENASLLPWLTGTAYLHSVLVQERRG
                     MLRVWNLSLLIATFSLTILGTFLTRSGVLNSVHAFSESAIGPYLLGFFGVVVAVSLAL
                     IAWRGDRLRSPGTMDSPLSREGAFLANNVVFALFAFVVLLGTVFPLIVQVLDDREIRV
                     GAPFFDRLGIPIGIMLLFLMAVAPVLPWRKASQELLRDRLFWPAWCGVGAVAVAVLAG
                     ADGLAPLVAFGLAGFAAGAALRQLVLATRRQGMRGLVGRANGGMIVHLGVILIAVGLT
                     ASNAYTRTQSFRLDRGETVTFAGHTFTYVDHEAFDTRAKFGVRALIEIDGEQTYAPAI
                     TTYKGRGASVPTPSVRTGFTNDIYLTLGDPAPQNPDDPARISVSIKPMMLWLWVGGLM
                     MAVGTLLSAFPGRRRRQPTDAVSAPVRNVREPEPELEASVPGG"
     gene            3702..>3945
                     /locus_tag="VIX62_12130"
     CDS             3702..>3945
                     /locus_tag="VIX62_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861025.1"
                     /translation="MAESSPVETLPEVEPRGGGGEPERPRRIAPFVALAVAVVFAIFF
                     TILAGSKTRSNQSNADTPLLGQAAPTIVGDTLDGGTF"
BASE COUNT          506 a         1345 c         1348 g          746 t
ORIGIN      
        1 ttggccgccg tcggtacgct ctacggcgga ctcaccgctg gggcgaaggg tcgggagacg
       61 ctgctcccgc tcctgctgct cccggtggtg gcgcccgtac tcatcggtgc cacccgggcc
      121 tgcgaagcag cgttcgggac cggcggggcg gcggtctccg aaggctggcc ctggttcggc
      181 ttgctggtgg tgttcgcagc aacgttcggt gcggtgggaa cggtcgcatt cggacccctg
      241 ttggacgagt agcacccgaa caggtctact gatggtcgat accctcgata ctgagcggcg
      301 cccgccgcga ggagaaagca gcatggcgtc cactgccgac caactcagca gtacgaggaa
      361 ggaaaccagt ccggctcgtc gctcatcgcc gaccggcacg cgcgccacgc gcctgctcgg
      421 cctcgccgtc atcgccggat tgctgtggct gctcgtcttc ggcctcgtgc tctcgccggg
      481 tgacgtcgtc cagcaggatt ccgtgcggat catgtacgtg cacgtcccct cggcgtggct
      541 cgcctacctc gcgttcatcg tcacggcgat tgcgtcggcg gcctacctct ggaggcgaac
      601 ccgctcgctg gcgtgggacc gaattgccgg tgcatcggcc gagatcggcg tgctcttcat
      661 ggcgatcaca ctcgtcaccg gctccctgtg gggcagcgtc acctggggca cgtactgggt
      721 gtgggacgca cggctgacca cgacggcctt cctcttcgtc acctacgtcg gctatctcgc
      781 cgtgcgtcgc ctcggtggct cgcacgaggc gcgggcgcga cgctccgccg tgctggcgct
      841 gctcgccgtc ctcgagatcc cgctcgtgca cttcagcgtc gtgctctggc ggcccttgca
      901 ccaggaggca tcggtcgcca acccgaccgg cgacgtcgag atggacggat tgatgctctt
      961 cagcctcttc gtcggcatca ttgcgttcac cctgctctac gtgtggctgc tcctccatca
     1021 gcagcgcgtg ctggcgatgg aggatgcgat cgacgaccac ggtctcgatg tcgcgctcgc
     1081 cgaacgcgtc aaggagggga tgtgaacgac gcaggggtga tcatcggcag ctacgcgttc
     1141 accatcgtgg ccatcggcgg ctacgtggcg tggatgctgc gccgggcccg ccggctatcg
     1201 gtgctcgtcc gggacgagga ccgtccgtgg acctgagccc gcgcaccacc acggcgctcg
     1261 acgagccgtc gccgccagcg cggcgctcga agcgctgggg gccctacgtc gtcctcggca
     1321 tcgtcctgat cggcggtgcc gtcgtcgtgt cgaagttcct cacctcggcg atcgactact
     1381 actgcaacgc cgatgaagtc ggcgtctcgg cgaagtgctc cggggatcgc cggctgcggg
     1441 tgcagggcaa ggtcgaggag ggatcgctcg tcgaggggac caatggcagc gtcgagtcgt
     1501 tcgtggtgtc gttcaacggc gccgaggtgc cggtcgacta ctcgtcgggc cagtcgctgc
     1561 ccgacctgtt ccaggagtgc atccccgtcg tcgtcgccgg gcgcttgcgc gacggccgct
     1621 tcgagggcac gagcgtcgaa gtgaagcact cgaacgagta cgaggaggac aacgccgatc
     1681 gcatcgacca ggcggagagc gcagcgtgct tgcagcaagc ctgaacggat cgatcggtgt
     1741 tgccgcgctc ctcgtcggcc tgacggcctc ggtcttcggc gccgtcatgc tcgtgttcgg
     1801 catccgccgc ggcgacgctc gggtcgtcgc ctccggccac cggtacgcat gggtggtggc
     1861 gttcaccggt gtcctggcgt tcgcggccat ggaacgggcg ctcatcaccc gggatttctc
     1921 gctgaagtac gtccaggagg tcggctccag cgccacgccc gcgctgtaca acttcaccgc
     1981 gctgtggagc gcgctcgaag gttccttgtt gctatggatc ctcgtgctcg gcggctacac
     2041 ggccgccatc gcgctccgct accgcgcccg ccgcaccgat ccgctcgtcg cgtgggcgat
     2101 ggtcgtgctg ctcgtgatct gcgcgttctt ctactcgttg acgctcttcg cctcgaaccc
     2161 gttcgcctcc gcggcgacgc tcgtcgagga cgggcggggc ccgaaccagc tgctgcagaa
     2221 ccatgtgctc gtgctgttcc acccgccgat gctctacctc ggctacgtcg gcatgagcgt
     2281 ccccttcgcg ttcgccatcg ccgcactcgt gaccggtcgc gtgggagagg gctggctgct
     2341 cgagacgcgg cggtggacgc tcttcgcctg gggctttctc acggccggca tcatcctcgg
     2401 cggctggtgg agctacgagg tcctcggctg gggcggcgtc tggggatggg atccggtcga
     2461 gaacgccagc ctcctaccgt ggctgaccgg cacggcgtat ctgcactccg tgctcgtgca
     2521 ggagcggcgg gggatgctgc gggtctggaa cctctcgttg ttgatcgcca cgttcagcct
     2581 cacgatcctc ggcacgttcc tcacccgttc cggcgtcctg aacagcgtcc acgcgttcag
     2641 cgagagcgcc atcgggccgt acctgctcgg cttcttcggc gtcgtggtgg cggtgtcgtt
     2701 ggcgttgatc gcgtggcggg gcgaccgtct gcgttcaccg ggcacgatgg actctcccct
     2761 ctcgcgggaa ggcgcgttcc tcgccaacaa cgtcgtcttc gccctctttg cgttcgtggt
     2821 cctcctgggc accgtgttcc cgttgatcgt gcaggtgctc gacgatcgcg agatccgcgt
     2881 cggcgcgccg ttcttcgacc gactgggcat cccgatcggc atcatgctgc tgttcctcat
     2941 ggcagtggcg ccggtgctgc cgtggcgcaa ggcgtcgcag gagctcttgc gggatcggct
     3001 cttctggccg gcgtggtgtg gcgtcggcgc ggttgctgtc gccgtgctcg ccggtgccga
     3061 cgggttggcg ccgctggttg cgttcgggct cgccgggttc gccgccggtg ccgcgctgcg
     3121 tcagctggtg ctcgccaccc gccgtcaggg catgcgcggt ctggtcggcc gggccaacgg
     3181 cgggatgatc gtgcatctcg gcgtcatctt gatcgccgtc ggcctcaccg cctcgaacgc
     3241 ctacacccgg acgcagagct tccgcctcga ccgaggggag accgtcacct tcgccggaca
     3301 cacgttcacc tacgtcgacc acgaggcgtt cgacacgagg gcgaagttcg gcgtccgggc
     3361 gctgatcgag atcgacggcg agcagaccta tgcccccgcc atcacgacgt acaaagggcg
     3421 tggtgcctcc gtgcccacac cgtcggtgcg taccgggttc acgaacgaca tctacttgac
     3481 gctcggcgat ccggcgccgc agaaccccga cgatccggcc cgcatctccg tgtcgatcaa
     3541 gccgatgatg ctgtggttgt gggtcggtgg cctgatgatg gcggtcggca cgcttctgtc
     3601 tgccttccct ggtcgacggc ggcgccagcc gaccgacgcc gtctccgctc ccgttcggaa
     3661 tgtgcgcgag ccggagccgg agctcgaagc gagcgttccc ggtggctgag tcgtctcccg
     3721 tcgagacgtt gccggaggtg gagccgcgcg gcggcggagg tgaacccgag cgccctcgcc
     3781 gaatcgcgcc gttcgtcgcg ctcgcggtcg ccgtcgtgtt cgcgatcttc ttcacgatcc
     3841 tggccggttc gaagactcgc tcgaaccaga gcaacgccga cacgccgttg ctgggtcaag
     3901 cggcaccgac gatcgtgggc gacaccctcg acgggggaac gttcg
//
LOCUS       DATDBZ010000524        11174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_3036938, whole genome shotgun sequence.
ACCESSION   DATDBZ010000524 DATDBZ010000000
VERSION     DATDBZ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11174
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3036938"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VIX62_12135"
     CDS             <1..455
                     /locus_tag="VIX62_12135"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEY5861026.1"
                     /translation="FLRMLSRRALDLKPPTGFVRDLVVEAKGEHAGRLDIKHGGITIV
                     TNLARTWAIRAGATQKGTLARLDAATHAAQDPLDTDVARELSEAFHFLWEIRLRHQAE
                     QVAAGQVPDDYVDPASLGPFSRSGLKEAFRVITRAQRLLASEEGIRPS"
     gene            complement(475..1254)
                     /gene="fabI"
                     /locus_tag="VIX62_12140"
     CDS             complement(475..1254)
                     /gene="fabI"
                     /locus_tag="VIX62_12140"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY5861027.1"
                     /translation="MLLQDKKLLITGVLTPQSIAFAAARAAQEQGAEIVLTNFGRTVS
                     LTEKTAKRLPTTPDVLEMDINEPDQIAAVCEELRGRWGRLDGFLHAIAFAPQDALGGN
                     FLNTEWDSVATAIKTSAFSLKALATGMLPLMESEGGAIVSLDFDGQVAWPIYDWMGVA
                     KAALEGVSRYLARDLGPKQIRVNTVSAGPLKTMAGKGIPGFDQISEAWGRRAPLGWDL
                     TDPTPVGNAVAFLLSDMAAGITGEVVHVDGGFHAMGTDLAL"
     gene            complement(1266..1583)
                     /locus_tag="VIX62_12145"
     CDS             complement(1266..1583)
                     /locus_tag="VIX62_12145"
                     /inference="COORDINATES: protein motif:HMM:NF025590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEY5861028.1"
                     /translation="MASRTVRLFAVAALVVAAGACSKKLDTEGLEPQLQDQIEAETGS
                     TITGVDCPSDIEVEAGTTFECTATEESGATFTVEVVQTDDAGRVSWRFKSAQPGATGA
                     TGA"
     gene            1681..2427
                     /locus_tag="VIX62_12150"
     CDS             1681..2427
                     /locus_tag="VIX62_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861029.1"
                     /translation="MTMRAVSFGQRPDLDERWDETVGPAWPEFLLHGSTVNQHWHHLF
                     DDHPAFQLYLIDETTDEVLGVANTLPFAWDGEIEHLPGGVDDVIVLAVAQHAEGIEPT
                     TLSAMQAVVTPGNRGRGLAGVLLHEMRRLAADAGFADLLAPVRPNWKARYPLTPFERY
                     CAWTREDGLPYDPWLRTHVRAGARFAVPAMASQRVDGTIAEWETWTGLTFPDSGDYVV
                     DGALIPVSIDRDADRGTIVEPNYWMRHPIR"
     gene            complement(2412..3338)
                     /locus_tag="VIX62_12155"
     CDS             complement(2412..3338)
                     /locus_tag="VIX62_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861030.1"
                     /translation="MAVRSFPPKLVAVAAVVVLAGLVLATLAWPRGSGDAAPRTLVRA
                     DPSTAPFQGLGVWVDIYDEPAWADPAAAAADMSARGVRTLYLQSSNAARSSAFVFPEA
                     TAAFLDAAHDEGMRVVAWYLPHLTDLVEDRARVRDVLDYTTPRGDRFDGFALDIESDA
                     VHDPVRRTERLVRLSSELRAEAGDQYPLGAIVPSPLRLRDDLAYWPGFPWHELALTYD
                     AILPMTYFTFRAHGPAETIEYVTGCIDEIRDRVGSDQVPIHVIGGLARDESASEALAF
                     SRAVREREVIGASTYTWPYVTEDQWSALQRIG"
     gene            3400..4113
                     /locus_tag="VIX62_12160"
     CDS             3400..4113
                     /locus_tag="VIX62_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5861031.1"
                     /translation="MDLGLTDTVAIVTASSKGLGLGTAKALSAEGAKVVLCARDEARL
                     RAAADALPGETLAIAADVTDPATPARLVDAALERFGALHVVVANAGGPPQARALETDD
                     EGMLAAVNANMLTSIRLVDAAVPHLRAAGWGRICLITSNAVKQPIPTLAYSNASRAGL
                     WAWAKTAAQDLIDDGITLNLACPGLHATDRVTELGHTGRLGDPVEFGKVVTFLCSRPA
                     GFVSGAALQVDGAGTLGLL"
     gene            4150..5793
                     /locus_tag="VIX62_12165"
     CDS             4150..5793
                     /locus_tag="VIX62_12165"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY5861032.1"
                     /translation="MNDTERARDLADRYWLQLLELEPTLGTEAGDERFDDRLGDPSEA
                     GRADAEAVHRGALAELVTIDRTAVEPTQRSTIDVMEAIATRFLADLEHRMDLLHVANH
                     FWGPAQLLGEVSSLQRTDTPERLDRFDARLRAFAPYLDAYAEVAREGVASGVTSPALV
                     VDRSMAQIERILALDAEHSPALAAAPDDPVAQERILEAVREVVNPAYAGFLEVLREYR
                     PHATESVGLSALADGDARYASQVLAWTTLPLDPAEVHQLGLDRFEAIQAERRDIAAGL
                     GFDDPASAIAARTASGENTAASPEALVQLAASQVARSWEAAPAWFGTLPSANCQVRRV
                     EPFREADMAWAFYNAPTEDGSRPGQYYINTYELEERAIHQLASVTYHESNPGHHFQIA
                     LEQEIVGRPALLRFGGGLAGSAFCEGWGLYSERLADEMGLYVDEWERLGMLDNQAHRA
                     ARLITDTGIHALGWSRDDAVATLRQSGLPQTDAEIEADRYVAIPGQALSYMVGMIEIE
                     QARARAATRDGASFVLRDFHDRVLALGQIPLPAFRREMA"
     gene            5845..6522
                     /locus_tag="VIX62_12170"
     CDS             5845..6522
                     /locus_tag="VIX62_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEY5861033.1"
                     /translation="MDVEITFSPAFALAEIRIAPGSEVKAEAGGMTSMSGGVEIETKV
                     TGGIMSGLKRSVLGGESFFINTFSAPEGGTLTVSPTLPGDIVHMPLNGTETMMVQSGS
                     WLASDTTVDIDTKWGGAKTFFSGEGLFLLKCTGQGDMLVSSYGAIVDRTLQAGEVYKI
                     DTGHIVAFEEGIGYQVNKVGGWKSTLLSGEGLVATFTGPGKLWMQTRSPSDLVHWLIP
                     QLPNKSN"
     gene            complement(6526..7737)
                     /locus_tag="VIX62_12175"
     CDS             complement(6526..7737)
                     /locus_tag="VIX62_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5861034.1"
                     /translation="MSEPRSDVRTTDRGMWLVVARRDFWVRLRDRGFVISTAITLIVL
                     SIFILLGAFSGDEASSFRLGLAGPDAKTVADAADFETAGERRRVEVTTVAYDDVAAAE
                     QALRDGEIDAALVHGDDLEGRTSVPPELAQVVQDALVRGRIQAALADAQVPASTIDEV
                     LNQPAIQIRTLEPVDEHRDENAGIAFIGVLLAYGQLFGYGVWVATGVLEEKSSRVVEV
                     LLSAIRPRQLLTGKIAGIGALGICQLVFIAAFAIGLSLITGAIDLPATAIGTALIVLT
                     WFVMGFAFYAGLFAVSGSLVSRMEELQNAMVPINLLIFASFFISIGALENPDSTLSVV
                     ASILPFSSALAMPVRIVLGSATPPQIVASIAALLISTAITIPLSGRLYAGAILRTGAR
                     VKLKDAWRAAA"
     gene            complement(7734..8627)
                     /locus_tag="VIX62_12180"
     CDS             complement(7734..8627)
                     /locus_tag="VIX62_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5861035.1"
                     /translation="MLELIDLRRRFGDVVALDGVSFSVPEGSIVGFVGPNGAGKTTAM
                     RIVLGVLEPDEGQVRWRGQPVEAVTRRRFGYMPEERGLYPKMRVQEQLVYLARLHGLS
                     ATDAHRQARETMEVLGVDGRADDRVESLSLGNQQRVQLAAALVHEPDVLVLDEPFSGL
                     DPVGVDVLAGVLRRQTVNHGVPVVFSSHQLELVERLCDAVVLIDGGRIVAHGSIDELR
                     ASRERRLLRIEIAGAPEGWFESVPGVALESEVAGGVVLLLEDGVDEQRVLDLARASGD
                     VTHFSRVRPGLAELFREVVGK"
     gene            8749..9312
                     /locus_tag="VIX62_12185"
     CDS             8749..9312
                     /locus_tag="VIX62_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861036.1"
                     /translation="MGSNAATELPDLDLGDELVEADATAETGEPAPSPVVVLYFDLAG
                     LPGWGSEAQPSDPDTSTILERYLGPLAAEIRLVQGYTALAMVHDAHSAILATMDLLGE
                     AARSSGSATAGAHITQDALEDVGPASRAARTAAALAGLAEADRLLVTADVIGALRPAD
                     GSTLSFDTGSPADVDGVRLSTYVVRRR"
     gene            complement(9322..9918)
                     /locus_tag="VIX62_12190"
     CDS             complement(9322..9918)
                     /locus_tag="VIX62_12190"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY5861037.1"
                     /translation="MRITVFGATGRTGRLLVALAMERGHEVTAFLREGSDPAPVAEAR
                     VAFGDPSDPAAIAAALAQARPEAVISAIGPIAGVTETEVSEAIGAIVAVMTDAGPQRL
                     VIASNVVVFSDDELTGEFADVGAEHRRNLAILRDSALDWTALAPELLRDDSATGSFVA
                     KVEAPPPGPTIARGDLALALLDALDRPDWIGRAIGISS"
     gene            complement(9933..>11174)
                     /locus_tag="VIX62_12195"
     CDS             complement(9933..>11174)
                     /locus_tag="VIX62_12195"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY5861038.1"
                     /translation="EFVRVSLGGIHDEAEIRGHRRTYVGALPGRIVRALKDAGSKNPV
                     MMLDEIDKVGTDWRGDPSSALLEVLDPAQNHTFRDHYLDVDLDLSEVLFITTANVAET
                     IPGPLFDRMEVIRIDGYTEEEKVAIAKHHLVARQRERNGLREDEVVFTDDALRVIVGD
                     YTREAGVRNLERELGKALRKVATTIAAGDTETPLTIDAADVRTYLGRQKFFFEAADRT
                     AVPGVATGLAVTGVGGDVLFIEASREDDGEPRDGLTLTGQLGDVMKESAQIALSYVRS
                     HAADLGIEPQRLRGRFHVHVPAGAVPKDGPSAGVTMTTALVSLLTDTPVRSEVGMTGE
                     VTLQGRVLPIGGLKQKVLAAHRAGLTEVILPKRNEGDLDDVPEQVREQMTFHVAEDIG
                     QVLAVALGAHNGAEVARPDAA"
BASE COUNT         1727 a         3990 c         3858 g         1599 t
ORIGIN      
        1 agttcctccg gatgctgtcg cggcgcgcgc tcgacctgaa gccgccgacc gggttcgtgc
       61 gcgacctggt ggtggaggcc aagggcgagc acgccggacg gctcgacatc aaacacgggg
      121 ggatcacgat cgtgaccaac ctcgcgcgca cgtgggcgat ccgcgccggt gccacgcaga
      181 agggaaccct cgcccgcctg gatgccgcca cgcacgccgc gcaggacccg ctcgacaccg
      241 acgtcgcgag ggagctgtcc gaggccttcc acttcctgtg ggagatccgg ctccggcatc
      301 aagccgaaca ggtggccgcg ggccaggtgc cggacgacta cgtcgatccg gcgtcgctgg
      361 gaccgttctc ccgcagcggc ctgaaggaag cgttccgggt gatcacgcgt gcgcagcgcc
      421 tgttggcgag cgaagagggc atccgtccgt cctgacgcgg gtcagcacgg ttgatcagag
      481 cgccaggtcc gtccccatgg cgtggaagcc gccgtccacg tgcacgacct cgccggtgat
      541 gcctgcggcc atgtcggaca acaggaacgc caccgcgttg ccgaccggcg tagggtcggt
      601 gagatcccag ccgaggggcg cacgtcggcc ccacgcctcc gagatctggt cgaacccggg
      661 gatgcccttg cccgccatcg tcttgagcgg tcccgccgag accgtgttga cccggatctg
      721 cttggggccg aggtcgcgcg cgaggtagcg cgacacccct tcgagcgccg ctttcgccac
      781 gcccatccag tcgtagatcg gccaggccac ctgcccgtcg aagtccagcg acacgatcgc
      841 gccgccctcg gactccatca acggcagcat ccccgtggcc aacgccttca aggagaacgc
      901 gctcgtcttg atcgcggttg ccacgctgtc ccattccgtg ttgaggaagt tcccacccaa
      961 cgcgtcctgc ggcgcgaacg cgatcgcgtg caggaacccg tcgagccggc cccaccggcc
     1021 gcgcagttcc tcgcagacgg cggcgatctg gtccggttcg ttgatgtcca tctcgagcac
     1081 gtcgggcgtg gtgggcagcc gcttggccgt cttctcggtg aggctcaccg tccgcccgaa
     1141 gttggtgagc acgatctcgg cgccctgctc ctgagcggcg cgagccgccg cgaacgcgat
     1201 cgactgcggc gtgagcacgc cggtgatgag cagtttcttg tcctggagca gcacgcccaa
     1261 tcctcctagg cgccggtggc gccggtcgcg ccgggctggg cggacttgaa cctccagctc
     1321 accctgccgg cgtcgtccgt ctgtacgacc tcgaccgtga acgtggcgcc cgactcctca
     1381 gtggcggtgc actcgaacgt ggtgccggcc tccacctcga tgtcgctggg gcagtcgacg
     1441 ccggtgatcg tgctcccggt ctccgcttcg atctgatcct gcagctgggg ttcgaggcct
     1501 tctgtgtcca gcttcttcga gcaggctcca gccgcgacga cgagcgccgc gacggcgaac
     1561 agtcgaacgg tccgtgaggc catggggtgg aggtcctcct cgaaggtgtg tggctgcgcg
     1621 ggtcctgggg agcctatcgg cggcccgagc gcagggcaaa cagaccgata ggctcacccg
     1681 gtgacgatgc gcgccgtcag cttcggccag cgaccggacc tggacgaacg ttgggacgag
     1741 accgtcggcc cggcctggcc ggagttcctg ctgcacggca gcacggtcaa ccaacactgg
     1801 catcatctct tcgacgatca tccagcgttc cagctgtacc tgatcgacga gaccaccgac
     1861 gaggtcctgg gtgtggccaa caccctcccg ttcgcgtggg acggcgagat cgaacacctt
     1921 ccgggtgggg tcgacgacgt catcgtcctg gccgtcgccc agcacgccga ggggatcgag
     1981 cccaccaccc tgtcggcgat gcaggcggtg gtgactccag ggaaccgcgg acggggcctg
     2041 gccggcgtgc tgctgcacga gatgcgccgc ctcgcggctg acgccgggtt cgccgatctc
     2101 ctcgccccgg tccggccgaa ctggaaggca cgctatccgc tcacgccgtt cgagcggtac
     2161 tgcgcatgga cccgtgagga cgggttgccg tacgacccgt ggttgcgcac ccacgtgcga
     2221 gccggcgcga ggttcgcggt gccggcgatg gcctcgcaac gggtcgacgg cacgatcgcc
     2281 gagtgggaga cctggacggg cctcacgttc cccgacagcg gcgactacgt ggtggacggc
     2341 gcgctcatcc cggtctcgat cgatcgcgac gccgatcgag gcacgatcgt tgaaccgaac
     2401 tactggatgc gtcacccgat ccgctgaagg gccgaccact ggtcctcggt gacgtagggc
     2461 cacgtgtagg tactggcgcc gatcacctcg cgttctcgga cggcgcgcga aaaggccagc
     2521 gcctcggagg cactctcgtc gcgggcgagc ccgccgatca cgtggatcgg gacctgatcg
     2581 ctgcccaccc gatcgcggat ctcatcgatg caccccgtca cgtactcgat ggtctccgcc
     2641 ggtccgtgcg cgcggaacgt gaagtaggtc atgggcagga tcgcgtcgta ggtgagcgcg
     2701 agctcgtgcc acgggaagcc gggccagtac gcgagatcgt cccgcagccg gagcggcgag
     2761 ggcacgatgg cgcccagcgg gtactggtcc cccgcctcgg cgcgcagctc cgacgagagc
     2821 cgcaccagcc gctcggtccg cctcacgggg tcgtgcaccg catcggactc gatgtcgagc
     2881 gcgaacccgt cgaaccggtc gccgcgcggc gtggtgtagt cgagcacgtc gcggacccgc
     2941 gcccggtcct ccaccaggtc ggtgagatgc ggcaggtacc aggccacgac ccgcatcccc
     3001 tcgtcgtgcg cggcatcgag gaaggcggcg gtcgcctccg ggaacacgaa cgcggacgag
     3061 cgggcggcat tggacgactg caggtacagc gtgcgcaccc cgcgcgccga catgtcggcc
     3121 gccgcagcgg cgggatcggc ccacgccggt tcgtcgtaga tgtcgaccca caccccgagg
     3181 ccttggaacg gggccgtcga gggatcggca cgaaccagcg tgcgtggggc ggcgtcgccg
     3241 ctgcctcggg gccacgccag cgtcgccagc acgagaccgg ccagcacgac cacggccgcc
     3301 acggccacga gcttgggggg gaaggagcga accgccatgt cccatcagcg tagccatggt
     3361 tcgcgcggac gcgtcccggc cccgtaccat gcccgggtca tggacctggg cctcaccgac
     3421 accgtcgcca tcgtcaccgc ctcttccaag gggctcggac tgggaaccgc gaaggcgctg
     3481 tcggcggagg gggcgaaggt ggtcctgtgc gcccgcgacg aggcgcggct gcgggcggcg
     3541 gcggacgccc tgccgggcga gacgctcgcg atcgccgccg acgtcaccga ccccgccacg
     3601 ccggcgcgac tggtcgatgc cgcgctggaa cgcttcggag cgctgcacgt ggtggtcgcc
     3661 aacgccggtg gtccccctca ggcacgcgcg ctcgagaccg atgacgaggg catgctggcc
     3721 gcggtcaacg cgaacatgct gacgtcgatc cgtctggtgg acgccgcggt cccccacctc
     3781 cgcgcggccg gctgggggcg catctgcctg atcacctcga acgccgtgaa gcagcccatc
     3841 cccacgctcg cctactcgaa cgcttcgcgc gccgggctgt gggcgtgggc gaagaccgcc
     3901 gcccaggatc tgatcgatga cggcatcacg ctgaacctcg cgtgcccggg cctgcacgcc
     3961 accgaccggg tcaccgagtt gggccacacc ggccgcctgg gcgaccccgt cgagttcggc
     4021 aaggtggtga ccttcctgtg ctcgaggccc gcgggcttcg tcagcggggc ggcgctccag
     4081 gtggacggcg cagggaccct gggcctgttg tgaccgctgc tcgcccgctg acacggaagg
     4141 accacaggag tgaacgacac cgaacgcgcc cgcgacctcg ccgatcgcta ctggctacag
     4201 ctgctcgagc tcgaacccac cctgggcacg gaggccggcg acgaacgctt cgacgaccgt
     4261 ctcggcgatc ccagcgaggc gggtcgggcc gacgccgagg ccgtgcaccg cggagcactc
     4321 gcagaactgg tcacgatcga ccggacggcg gtcgagccca cccagcggag caccatcgac
     4381 gtgatggagg cgatcgccac ccggttcctc gctgatctgg aacaccgcat ggatctgctc
     4441 catgtcgcca accacttctg ggggccggcg cagttgctgg gtgaggtgag ctcgctgcaa
     4501 cgcacggaca cgcccgagcg gctcgatcgg ttcgacgcgc ggctacgggc cttcgctccg
     4561 tacctggacg cctacgcgga ggtggcgcgt gaaggggtgg cctccggcgt cacctccccg
     4621 gcgctggtgg tcgatcgcag catggcccag atcgaacgga tcctggccct cgacgccgag
     4681 cactcacccg ctctggcggc ggcgcccgac gatcccgtcg cccaggagcg gatcctcgag
     4741 gcggtccgcg aggtcgtgaa ccccgcctac gccggcttcc tggaggtcct gcgcgagtac
     4801 cgcccgcatg ccaccgagtc ggtgggactg tcggcgctcg ccgacggcga cgcgcgctac
     4861 gcgtcccagg tgctggcatg gaccacgttg ccgctggatc cggccgaggt gcaccagctg
     4921 gggctggacc ggttcgaggc gatccaagcc gagcgccgcg acatcgccgc cgggctcggg
     4981 ttcgacgacc ccgcctcggc gatcgcggcg cgcaccgcct cgggagagaa cacggccgcc
     5041 tcccctgagg cgttggtgca gctggcggcg tcgcaggtgg cccgcagctg ggaggccgcg
     5101 ccggcgtggt tcggcacgct gccgagcgcc aactgccagg tgcgccgcgt ggaaccgttc
     5161 cgggaggccg acatggcgtg ggccttctac aacgctccca ccgaggacgg ctcccggccg
     5221 gggcagtact acatcaacac ctacgagctg gaggaacgag cgatccacca gctcgccagc
     5281 gtcacgtatc acgagtccaa ccccggccac catttccaga tcgccctcga acaggagatc
     5341 gtggggcgac ccgcgttgct gcggttcgga ggcgggctcg ccggcagcgc gttctgcgag
     5401 gggtgggggc tgtacagcga gcgtctcgcc gacgagatgg ggctctacgt cgatgagtgg
     5461 gagcgcctgg ggatgctcga caaccaggcc caccgggcgg cgcggctcat caccgacacg
     5521 gggatccacg cgctgggctg gtcccgcgac gatgcggtgg cgacgctgcg ccagtcgggg
     5581 ctgccgcaga ccgacgccga gatcgaagcc gaccggtacg tcgcgatccc gggccaggcg
     5641 ctgtcctaca tggtcggcat gatcgagatc gaacaggctc gggcgcgggc ggcgacgcgc
     5701 gatggtgcct cgttcgtgct ccgcgatttc cacgaccgcg tgctggcgct cggccagatc
     5761 cccctgccgg cgttccgccg cgagatggca tgagcccggg ccgctaggct ctcgtcggca
     5821 ccgtcatcga gcaggaggca ccgcatggat gtcgagatca cgttcagccc cgcgttcgca
     5881 ctcgccgaga tccggatcgc gccggggagc gaggtcaagg ccgaggccgg cggcatgacc
     5941 agcatgtcgg gcggagtcga gatcgagacc aaggtgacgg gcgggatcat gtcgggcctg
     6001 aagcgctcgg tgctcggcgg cgagagcttc ttcatcaaca cgttcagcgc gcccgagggc
     6061 ggaaccctca cggtctcccc cacgctgccc ggcgacatcg tgcacatgcc gctgaacggc
     6121 accgagacga tgatggtgca gtcgggatcc tggctcgcct ccgacacgac ggtcgacatc
     6181 gacacgaaat ggggcggggc gaagacgttc ttctcgggcg agggcctgtt cctgctcaag
     6241 tgcaccggtc agggcgacat gctggtctcg agctacggcg ccatcgtgga caggaccctg
     6301 caggccggcg aggtctacaa gatcgacacc ggtcacatcg tcgcattcga agagggcatc
     6361 ggctaccagg tcaacaaggt gggcggctgg aagtccacct tgctgtcggg cgaaggcctg
     6421 gtggcgacgt tcaccgggcc ggggaagctc tggatgcaga cccgcagccc gagcgatctc
     6481 gtccactggt tgatcccgca gctgccgaac aagagcaact gaggctcacg cagcggcgcg
     6541 ccaggcgtcc ttcagcttca cgcgcgctcc cgtgcgcagg atcgcgccgg cgtacaggcg
     6601 ccccgacagc gggatcgtga tcgcggtcga gatcaacagc gccgcgatcg aggccacgat
     6661 ctgcggcggc gtggccgagc ccagcacgat gcgcaccggc atggccagcg ccgaggagaa
     6721 cggcaggatc gaggccacca ccgacagcgt ggagtccggg ttctccagcg cgccgatcga
     6781 gatgaagaac gacgcgaaga tcagcaggtt gatcggcacc atcgcgttct gcagctcctc
     6841 catgcgcgag acgagggagc ccgagaccgc gaacagcccc gcgtagaacg cgaaccccat
     6901 cacgaaccac gtgaggacga tgagggcggt gccgatcgcc gtggcgggca gatcgatcgc
     6961 cccggtgatc agcgagagac cgatcgcgaa cgccgcgatg aacaccagtt ggcagatgcc
     7021 cagtgcaccg atccccgcga tcttgccggt gagcaactgc cgcggccgga tcgccgacaa
     7081 cagcacctcg accacccggg aggatttctc ttcgaggacc ccggtggcca cccacacgcc
     7141 gtagccgaac agctgcccgt atgccagcag cacgccgatg aacgcgatgc cggcgttctc
     7201 atcgcggtgc tcatcgacgg gttcgagggt ccggatctgg atcgcgggtt ggttcagcac
     7261 ctcgtcgatc gtcgaggccg gcacctgggc atcggcgagc gccgcttgga tacgtccccg
     7321 gaccagggcg tcctggacga cctgagcgag ctccggcggc accgaggtgc gcccttccag
     7381 atcgtcaccg tggacgagcg cggcgtcgat ctcgccgtcg cgcagcgcct gctcggccgc
     7441 cgcgacatcg tcgtacgcga cggtcgtgac ctcaacgcgg cgccgctccc cggcagtctc
     7501 gaagtcggcc gcgtcggcga cggtcttcgc gtccggcccc gccagcccca gtcggaagga
     7561 ggatgcttca tccccagaga acgcgcccag gaggatgaag atcgacagca cgatgagcgt
     7621 gatcgccgtg gagatcacga acccccggtc gcgcaaccgc acccagaagt cgcgcctggc
     7681 caccaccagc cacatccccc ggtcggtggt gcgcacatcg ctccgcggtt cgctcacttg
     7741 cccaccacct cgcggaagag ctcggcgagg ccggggcgca cgcgcgagaa gtgggtcacg
     7801 tcaccggagg cccgcgcgag atccagcacg cgctgctcgt cgaccccgtc ttcgagcagg
     7861 agcaccacac cgccggcaac ctcggattcc agggccacgc ccgggaccga ctcgaaccat
     7921 ccctcgggcg cgccggcgat ctcgatccgc agcaggcggc gttcgcggct ggctcgcagc
     7981 tcgtcgatcg acccgtgcgc cacgatccgc ccgccgtcga tcagcacgac ggcgtcgcac
     8041 aaccgctcga ccagctcgag ctggtggctg gagaacacca ccgggacgcc gtggttcacg
     8101 gtctggcgac gcagcacgcc ggccaagacg tccaccccca ccgggtccaa gcctgagaac
     8161 ggctcgtcca gcaccagcac gtcgggctcg tgcacgagcg ccgccgcgag ctgcacccgc
     8221 tgctgattgc ccagggacaa gctctccacc cggtcgtcgg cgcgcccgtc gacgccgaga
     8281 acctccatcg tctcgcgcgc ctgccggtgc gcgtcggtgg ccgagagtcc gtgcaaccgc
     8341 gcgaggtaga ccagctgttc ctgcacccgc atcttggggt agaggccgcg ctcctccggc
     8401 atgtagccga accggcgacg ggtgacggcc tcgaccggtt gcccgcgcca ccggacctgc
     8461 ccctcgtccg gttccagcac gcccagcacg atgcgcatcg cggtggtctt gccagcgccg
     8521 ttgggcccca cgaaccccac gatcgagccc tcgggcaccg agaacgagac accgtccagc
     8581 gcgacgacgt cgccgaaacg gcgacgcagg tcgatcagct cgagcacggc gaggtcgggt
     8641 cgggcatggt ttccagcata ggtttggcag cataaggtcg ctgacgtcgt tggctcgcag
     8701 gcgatcggac gttccagtcc cgcgaccttc cggccgatac caaacccgat gggatcgaac
     8761 gcggccaccg agcttcccga cctcgacctc ggcgacgagc tcgtcgaggc ggacgcaacc
     8821 gctgagaccg gcgagccggc gccgagcccg gtggtcgtgc tgtacttcga cctcgccggg
     8881 ttgcccggct ggggctcgga agcccaaccc agcgaccccg acaccagcac gatcctggag
     8941 cgctacctgg ggcccctcgc cgccgagatc cggctcgtcc agggttacac cgccctggcg
     9001 atggtgcacg atgcccactc cgcgatcctg gccacgatgg atctgttggg cgaggcggca
     9061 cgttcgagcg gcagcgccac ggccggggcc cacatcacgc aggacgcgct cgaagacgtc
     9121 ggacccgcct cgagggccgc gcggacggcg gcggcgctgg ccggcctcgc ggaagcggac
     9181 cggctcttgg tgacggccga cgtgatcggt gcgctgaggc ctgccgacgg ctcgacgctg
     9241 tcgttcgaca cgggctcgcc cgccgacgtg gacggggtac ggctcagcac ctacgtggtg
     9301 cggcggcgct gagcgcgcgg ttcagctgct gatcccgatc gcacggccga tccagtcggg
     9361 gcggtcgagg gcatcgagca gggcaagcgc caggtcgccg cgagcgatcg tcgggccggg
     9421 gggcggcgcc tccaccttgg ccacgaaact ccccgtggcg ctgtcgtcac gcagcagctc
     9481 gggagccagc gcggtccagt ccaacgcgct gtcccggagg atcgcgaggt tccggcgatg
     9541 ctccgcgcca acatccgcga actcccccgt gagctcgtcg tcggagaaca ccacgacgtt
     9601 cgaggcgatc accagccgtt gcgggcccgc gtcggtcatc accgcgacga tggcaccgat
     9661 cgcctcggac acctcggtct cggtgacgcc ggcgatgggg ccgatcgccg agatcaccgc
     9721 ctccggccgg gcctgggcca gcgccgccgc gatcgcggcg gggtcggacg ggtcgccgaa
     9781 ggcgacccgt gcctcggcca ccggggcggg atcggatccc tcacgcagga acgcggtcac
     9841 ctcgtgaccg cgttccatgg ccagcgccac cagcaaccgg ccggtccgtc ccgtggctcc
     9901 gaacaccgtg atccgcacgg gcggcactgt acctaggcgg cgtcgggtcg cgcgacctcc
     9961 gccccgttgt gtgcgcccag cgcgacggcc aggacctgac cgatgtcctc ggccacgtgg
    10021 aaggtcatct gttcgcgcac ctgctcgggc acgtcgtcca ggtcaccttc gttgcgcttg
    10081 ggcaggatca cctcggtgag cccggcgcgg tgggcggcca gcaccttctg cttcagcccg
    10141 ccgatcggga gcacccggcc ctgcaaggtg acctcgccgg tcatgcccac ctccgaacgt
    10201 accggcgtgt cggtgaggag cgacaccaac gcggtggtca tggtgacacc ggcgctgggg
    10261 ccgtccttcg gcaccgcacc cgcgggcacg tgcacgtgga agcgaccacg cagccgctgg
    10321 ggctcgatcc ccaggtcggc ggcatgcgag cgcacgtagc tgagcgcgat ctgcgccgac
    10381 tccttcatca cgtcgcccaa ctggccggtg agggtcagcc cgtccctcgg ctcgccgtcg
    10441 tcctcacggc tcgcctcgat gaacagcacg tcgccgccca cgccggtgac cgcgagaccg
    10501 gtcgccacgc cggggaccgc cgtgcggtcg gccgcctcga agaagaactt ctggcgtccc
    10561 aggtaggtgc ggacgtcggc ggcatcgatc gtgagcggcg tctcggtgtc tcccgctgcg
    10621 atcgtggtgg ccaccttcct cagcgccttg cccagctcgc gttcgaggtt gcggacgccg
    10681 gcctcgcggg tgtagtcgcc cacgatcacg cggagcgcgt catcggtgaa cacgacctcg
    10741 tcctcacgca ggccgttccg ctcgcgctgg cgcgccacca ggtggtgctt ggcgatcgcg
    10801 accttctcct cctcggtgta gccgtcgatc cggatcactt ccatccggtc gaacaatggc
    10861 ccggggattg tctcggcgac gttggccgtg gtgatgaaca gcacctcgga caggtcgagg
    10921 tcgacgtcga ggtagtgatc gcggaacgtg tggttctgcg ccgggtcgag cacctccagg
    10981 agcgcgctcg aggggtcgcc gcgccagtcg gtgcccacct tgtcgatctc gtcgagcatc
    11041 atcaccgggt tcttcgaacc ggcgtccttc agggcgcgga cgatccggcc gggcagggcg
    11101 cccacgtagg tgcgccggtg cccgcggatc tcggcctcgt cgtggatgcc gcccagcgac
    11161 acgcgcacga actc
//
LOCUS       DATDBZ010000525         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_577122, whole genome shotgun sequence.
ACCESSION   DATDBZ010000525 DATDBZ010000000
VERSION     DATDBZ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577122"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2083)
                     /locus_tag="VIX62_12200"
     CDS             complement(<1..>2083)
                     /locus_tag="VIX62_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861039.1"
                     /translation="SQDGRNQEGGREEGRNQEGGNEEGGGHEDDTGTPDERTEDDMII
                     EEGKFLLYSHITPALKAGDYRFEVSQAMTAKKGTQNIDAGAVPVEALQTHVTVTAPRY
                     QLPPDQVLSTYPPAGTEGAYGSRLPQIVIKRRTLPWERGLKIPDGHGGQTNVAETTPW
                     LALVLIAEGEAELKLNQDVAKCVSSDVHLDGEQDVAKGNCLVVRQSILEKILPSRKDV
                     PLLAHARQVDIHDTELMMGDDDGFLSVVISNRLPVAGVDSAGKATPVKYLACLINLEG
                     QFHRLIPEAPAPRDHIELFNDIHAMVVQPAVYDHVVSGGLETNWNTSGLATSGQIHLD
                     APLTDSPHGDAPRAAGARADAPHATGMAIERNLVESAGPTMQAYTAQAGWAHKAKPDI
                     YEVMATDFRHEIVGVLDPQYTFPCLLHWSFVSTGSTTFETLMRELDSGLAGSLPQDPP
                     PPATHKRVPPTGREPLEVVETGHVGLVQRTRRGDSRRSWYRGPLLAHPSSGERLPIAH
                     ASDQLRAVIPDGREDISLAAAFEVGRLLALSKPAMVAALMRWRQAQFQAARSGALWDH
                     MRDRLPFELDDGGIRTMLEDKLRTLITTDPEQFIGAHRPLVTPGDPVDWGANPLTMLG
                     RGLGLGLEVDLGRQLREIDDFAAVSGTLRMTVPPVIGGLSLRGAVNPAISETLLTSLD
                     TITEQMVSNVLA"
BASE COUNT          285 a          686 c          732 g          380 t
ORIGIN      
        1 cgccagcacg ttgctcacca tctgctcggt gatcgtgtcg agcgacgtga gcaacgtctc
       61 ggagatcgcg gggttcaccg caccgcgcag gctgagaccc ccgatcacgg gtggcacggt
      121 catgcgcaac gtcccgctga cggcggcgaa gtcatcgatc tcgcggagct ggcgaccgag
      181 gtcgacctcg aggccgaggc cgagaccgcg gccgagcatg gtcaacgggt tggctcccca
      241 gtcgacgggg tcgcccggcg tcacgagggg gcggtgcgca ccgatgaact gttcgggatc
      301 ggtcgtgatc agcgttcgca gtttgtcttc cagcatcgtg cggatgccgc cgtcgtcgag
      361 ttcgaacggc agccgatcgc gcatgtgatc ccacaacgca cccgagcggg ccgcctggaa
      421 ctgcgcctgc cgccacctca tcaacgccgc gaccatggcc ggcttgctga gcgcgagcag
      481 gcggccaacc tcgaacgcgg cggcgagcga gatgtcctcc cggccgtcgg ggatgaccgc
      541 acggagctgg tcggaggcat gggcgatcgg cagccgttca ccgctgctcg ggtgcgcgag
      601 caggggcccg cggtaccagc tgcgcctgct gtcaccgcga cgcgtccgtt ggaccagccc
      661 gacatgtccg gtctcgacca cttccagcgg ttcccgaccg gtcggcggga cccgcttgtg
      721 cgtcgccggc ggtggaggat cctgcggcag gctccccgcg aggcccgagt cgagttcgcg
      781 catcagggtc tcgaacgtgg tcgacccggt cgacacgaag ctccagtgca gcaggcacgg
      841 gaacgtgtac tgcggatcga gcacgccgac gatctcgtga cggaaatcgg tggccatcac
      901 ctcgtagatg tccggcttcg ccttgtgcgc ccaacccgcc tgggcggtgt aggcctgcat
      961 ggtgggaccc gccgactcga ccaggttgcg ttcgatcgcc atcccggtgg cgtgtggagc
     1021 gtcggcacgc gcgcccgcgg cacgtggtgc gtcgccgtgt ggtgagtcgg tcaatggtgc
     1081 gtcgagatgg atctggccgc tcgtcgccaa cccgctcgtg ttccagttcg tctcgaggcc
     1141 accgctcacc acgtggtcgt acacggccgg ctgcaccacc atcgcgtgga tgtcgttgaa
     1201 caactcgatg tggtcgcgcg gcgcgggggc ctcggggatc agccggtgga actggccctc
     1261 cagattgatg aggcaggcga ggtacttcac cggtgtcgcc ttgccggcgg agtcgacccc
     1321 ggccacgggg agtcggttgg agatgacgac cgacaggaag ccgtcgtcgt cgcccatcat
     1381 cagctcggtg tcgtggatgt cgacctggcg ggcgtgggcg agcagcggca cgtccttgcg
     1441 cgacggcagg atcttctcga ggatcgactg gcgcacgacg aggcagttgc ccttggcgac
     1501 gtcctgctcg ccgtcgaggt gtacgtcgga gctgacgcat ttcgcgacgt cctggttgag
     1561 tttcaactcg gcctcaccct cggcgatgag gaccagcgcc agccacgggg tggtctcggc
     1621 gacgttggtc tggccgccgt ggccgtcggg gatcttcagg ccccgttccc aggggagtgt
     1681 ccgccgtttg atgacgatct gtgggagccg gctgccgtag gcgccctcgg tccccgccgg
     1741 cgggtacgtg gacagcacct ggtcgggagg gagttggtaa cgcggcgcgg tgacggtcac
     1801 gtgtgtctgc agcgcttcca cgggcacggc accggcatcg atgttctggg tgcccttctt
     1861 cgccgtcatc gcctggctga cctcgaagcg atagtcgccc gccttcaacg ccggcgtgat
     1921 gtggctgtag agcaggaact tgccctcctc gatgatcatg tcgtcctccg tccgctcgtc
     1981 cggcgtgccg gtgtcgtcct cgtggccgcc gccttcttcg ttgccgcctt cttggttgcg
     2041 accttcttcg cggccacctt cttggttgcg gccgtcttgg ctg
//
LOCUS       DATDBZ010000526         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_381456, whole genome shotgun sequence.
ACCESSION   DATDBZ010000526 DATDBZ010000000
VERSION     DATDBZ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381456"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VIX62_12205"
     CDS             complement(<1..1273)
                     /locus_tag="VIX62_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5861040.1"
                     /translation="MGGRGGGGGGFRPVDIDAQKKLNAEAPHIPHLGRRVVALFRPYR
                     FQILITGVLVIAGASIAVVPALIVQQIFDQALFPVDGGPPDLDLLLRLVLIMIGLFVL
                     SAGLGVVQTWFTSTVGNRVTGDLRVKLFEHLQAMELGFFTRTKTGVIQSRLQNDVGGV
                     SGVLTNTVTSILGNVVTVIASLVAMIIIDWRLTLIAVVMMPILVIVQRRVGQVRARIA
                     GETQESLSELTSITQETLSVSGILLSKSFNRQRTESARYSTENVNQVRLQVKRAMSGQ
                     GFFAVVQVLMASVPAVIYLVSGYLITGGTDMITAGTIVAFTTVQARLLMPLMGLMRVA
                     LDVQTSGALFARIFEYLDLRPAISDAPDAIRVADAPGPIGRIEFRDVVFRYPDAAKDA
                     RPTLHGVTFTAEPGQHVAFVGPSGAGKTTVLY"
     gene            complement(1363..>2180)
                     /locus_tag="VIX62_12210"
     CDS             complement(1363..>2180)
                     /locus_tag="VIX62_12210"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY5861041.1"
                     /translation="GTRMLSVPGVVSVVAIGVSSGGGVHDSHPVGGSHDEQQALNITG
                     SSDTAFPVHVKTCGMALSPVRLAVVTRTIVAALIGIAAGVVVAPFLGIAAGLLAGWSG
                     FAVTSVAWVAILIWPMDAAHTRAHATGEDPGRSVARLVALIGSVASLGAVTIVLIQTQ
                     NTSELESYLLAAIAVVSVAASWALIQADYMLRVAHIYYGDPVGGIDFNQDEDPSYTDF
                     AYFSLGIGMGYQVGDSAVRTNEIRRLVIAQSLLAYLFGAVIIGTVVNLVIDLG"
BASE COUNT          390 a          712 c          747 g          331 t
ORIGIN      
        1 ggtacagcac cgtcgtcttg cccgcgccgg acggccccac gaacgcgacg tgctggccgg
       61 gctcggccgt gaaggtcacg ccgtgcaggg tgggacgcgc gtccttcgcg gcgtccggat
      121 agcggaacac cacgtcgcgg aactcgatcc tcccgatcgg accgggagcg tcggccacgc
      181 ggatggcgtc gggcgcatcg ctgatcgcgg gcctcaggtc gaggtactcg aagatgcgcg
      241 cgaagagcgc gccggaggtc tgcacatcga gcgcgacgcg catgaggccc atgagcggca
      301 tcagcagccg ggcctgcacc gtggtgaatg cgacgatcgt tccggcggtg atcatgtcgg
      361 tgccgccggt gatcaggtag cccgagacca ggtagatcac tgcgggaacg ctcgccatga
      421 gcacctggac gaccgcgaag aagccctggc cgctcatggc ccgcttgacc tgcagacgta
      481 cctggttcac gttctcggtg ctgtagcggg cggactcggt gcgctgccga ttgaacgact
      541 tcgacaggag gatgcccgac acgctcagcg tctcttgagt gatcgaggtc agctccgaga
      601 gcgactcctg cgtctcgccc gcgatgcgcg cgcgcacctg gccgacccgt cgttgcacga
      661 tcacgaggat cggcatcatc acgacggcga tcagggtgag tcgccagtcg atgatgatca
      721 tcgcgaccag tgacgcgatg accgtcacca cattgcccag gatgctggtc acggtgttcg
      781 tgaggacgcc ggagaccccg cccacgtcgt tctgcaggcg cgactggatg acgccggtct
      841 tggtgcgcgt gaagaagccg agctccatcg cctgcaggtg ttcgaagagc ttgacccgca
      901 ggtcgccggt caccctgttg cccacggtcg aggtgaacca ggtctgcacg acgccgaggc
      961 cggccgaaag cacgaacagc ccgatcatga tgaggaccag tcgcagcagg aggtcgagat
     1021 cgggtgggcc gccgtcgacc gggaagagcg cctggtcgaa gatctgctgc acgatgagcg
     1081 ccggcaccac ggcgatggag gcgccggcga tcacgagcac gccggtgatg aggatctgga
     1141 accggtaggg gcggaacagc gcgaccacac gccgaccgag gtgcgggatg tgcggcgcct
     1201 cggcattgag cttcttctgg gcgtcgatgt cgaccgggcg gaatcctccg ccgccaccac
     1261 ccctgccacc catgctcacg tcggcaagcc tacttacggg ttcggacggg agggccggag
     1321 aagctgtccg cgtaatcgcc catcggccgc gacgccttcg cgtcagccga ggtcgatgac
     1381 caggttcacg accgtgccga tgatcaccgc gccgaagagg tacgcgagca gcgactgtgc
     1441 gatcaccagc cgccggatct cattcgtgcg aacggcgctg tcgccgacct ggtatcccat
     1501 gccgatgccg agggagaagt acgcgaagtc ggtgtaggac gggtcctcat cctggttgaa
     1561 gtcgatgccc cccacgggat cgccgtagta gatgtgcgcg acccggagca tgtagtcggc
     1621 ctgaatgagc gcccacgacg ccgccacgct gacgacggcg atcgcggcga ggaggtacga
     1681 ctccaattcc gatgtgttct gggtctggat gaggacgatc gtgacggcgc ccaggctcgc
     1741 gacgctgccg atcagagcga ccagacgcgc gaccgagcga ccggggtctt cgccggtcgc
     1801 atgtgcacgc gtgtgcgcgg cgtccatcgg ccatatcagg atcgcgaccc aggcgacgct
     1861 cgtcacggcg aagcccgacc agcccgccag cagtccggcc gcgattccca ggaagggggc
     1921 caccacgacc cctgcggcga tgccgatgag ggcggcgacg atcgtgcgcg tgacgacggc
     1981 gagccgcacg ggactgagag ccataccgca tgttttcacg tgaaccggga atgccgtgtc
     2041 agacgagccc gttatgttga gagcttgttg ttcatcgtgt gacccgccta cgggatggct
     2101 gtcgtgcacg ccgccccccg aggagacgcc tatggccacc accgacacca cccccggcac
     2161 cgacagcatc cgcgttccga
//
LOCUS       DATDBZ010000527         2652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_577886, whole genome shotgun sequence.
ACCESSION   DATDBZ010000527 DATDBZ010000000
VERSION     DATDBZ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577886"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1124
                     /locus_tag="VIX62_12215"
     CDS             156..1124
                     /locus_tag="VIX62_12215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5861042.1"
                     /translation="MTAFVRPIDAHFDALEDWPYRPHDHLWDDLRVRYVDEGPIEGPV
                     MLLLHGMPTWAYLYRDMIPGLVDAGYRCVAPDHLGFGRSDKPTDRAWYTIARHTEVLT
                     SLIAALDLDDVTLVCQDWGGPIGLAQAAKTPERFSRLVVMNTWLHHPDYEYTDGARGW
                     IGMWLPGGVFDRERPNVACVPLFSGELAPMSGLLTAVANGTEPVLDGAAARNYAGWAA
                     PFRDLPDDAFNGARQFPLSIPLNDPVAGNAMAQAEHYRTLLAWPKPAHFVWGGADPVF
                     TEPWGRAWAGRMGATFDVIPEANHFLQNTHGAEVAEHILRRIAGHR"
     gene            complement(1191..1871)
                     /locus_tag="VIX62_12220"
     CDS             complement(1191..1871)
                     /locus_tag="VIX62_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5861043.1"
                     /translation="MRTYGQYCPIARGAEIFAERWTPLIIRNLYLGCETFGEILDGAP
                     GLSRTLLTQRLSQLERFGIVESAPKAQGRGHRYRLTSSGHDLFRVCEALGEWGAQWLE
                     IAPENLDPFVALWSMCNALRRDRLPERRVVIRLDFTGFRPHERYWLLVEHGEAEICKT
                     YSGLDEDLYITADAEAFVKWHAGQLSWAEATRDRRIELDGPSWLVRAFPTWNGRSMFA
                     HIKPALGR"
     gene            2015..>2652
                     /locus_tag="VIX62_12225"
     CDS             2015..>2652
                     /locus_tag="VIX62_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5861044.1"
                     /translation="MNIPRFRGDMIASGDDAYDVARAVWNGSVDRHPRLIARCRGTAD
                     VAAAVKFARDQDLEIAIRGGGHNVAGTAVCDGGIVIDLSAMRAVSVDPVERTALVQGG
                     ALWGDVDHETQAHGLATTGGIVGHTGVGGLSLGGGIGWLMRKHGLTIDNLVEAEVVTA
                     RGDIIRVSADQHTDLFWALRGGGGNFGVVSSFRFALHPVGPLVMAGPVFWAA"
BASE COUNT          420 a          898 c          895 g          439 t
ORIGIN      
        1 actccggtag cacccacggt gcgccgatcc tgagtcatgc acatcgggcg atacgcccta
       61 tctcggcgct cgcggtgctc agatccgatc gaggcgcctt gacccgccca gtatcgggta
      121 gcgggcggtc gaagccccac cgctagcgtc tggcggtgac cgcgttcgtc cggccgatcg
      181 atgcacactt cgacgcgctg gaggactggc cgtaccgacc tcacgatcat ctctgggacg
      241 accttcgggt gcgctacgtc gacgagggtc cgatcgaggg acctgtgatg ctgctgctcc
      301 acgggatgcc gacgtgggcg tatctctacc gcgacatgat cccgggtctc gtcgacgccg
      361 gctatcgctg tgtggctccg gaccatctcg gcttcggtcg ctccgacaag cccaccgacc
      421 gggcgtggta cacgatcgcc cgccataccg aggtgctgac gtcgctgatc gcggcactcg
      481 acctcgacga cgtcacgctt gtatgccagg actggggcgg gccaatcggg ttggcgcagg
      541 cggcgaagac gccggagcgg ttcagccggc tcgtcgtgat gaacacctgg ctgcaccacc
      601 ccgactacga atacaccgac ggcgcccgcg gatggatcgg catgtggctc cccggtggtg
      661 tgttcgatcg cgaacgaccg aatgtcgcct gcgtgccact gttctctggc gaactggcgc
      721 cgatgagcgg gctgctgact gccgtcgcga acggtacgga gccggtgctc gacggcgccg
      781 cggcgaggaa ctatgccggc tgggcggcgc cgttccggga ccttccggac gacgcgttca
      841 acggagcgcg tcagttccca ctgtcgatcc cgctcaacga cccagtcgcc ggaaacgcga
      901 tggcacaggc cgagcactat cgcaccctgc ttgcctggcc gaagccggcg cacttcgtgt
      961 ggggtggggc cgatcctgtg ttcaccgagc cctggggtcg ggcatgggcc gggcgcatgg
     1021 gggccacgtt cgacgtgatc cctgaggcga accatttcct tcagaacacc cacggtgccg
     1081 aagtcgccga acacatcctg cggcgaatcg ccgggcatcg gtgatccgca cggtcgtcga
     1141 gcgactgcct cgcccactcc ccgggcccgg ccgccgcccg ttgaccgtcg tcaacggccg
     1201 agggcgggtt tgatgtgagc gaacatgctg cggccgttcc aagtcgggaa ggcgcgtacg
     1261 agccacgaag gaccgtcgag ctcgatccgg cggtcgcggg tggcctccgc ccaggagagc
     1321 tggccggcgt gccatttcac gaacgcttcg gcgtcggcgg tgatgtagag gtcttcgtcc
     1381 aggcctgaat acgtcttgca gatctcggcc tcaccgtgct cgaccagcag ccagtaccgc
     1441 tcgtggggtc ggaagccggt gaaatccaaa cggatgacca cgcgccgctc ggggagtcgg
     1501 tcccgacgga gcgcgttgca catcgaccac agcgccacga acggatcgag gttctcgggg
     1561 gcgatctcga gccactgagc tccccactcg ccgagagctt cacagactct gaacagatcg
     1621 tggcccgagc tcgtgaggcg gtatcggtgg ccgcgcccct gtgccttcgg tgccgactcg
     1681 acgattccga agcgctcgag ctgcgagagc cgctgcgtga ggagggtgcg ggagaggccc
     1741 ggcgcaccgt cgaggatctc gccgaaggtc tcgcacccca gatagaggtt gcggatgatc
     1801 aacggcgtcc aacgctcagc gaagatctcc gctccacggg cgatcgggca gtactggccg
     1861 taggtcctca tgagcgcagt gtcccgcgcc agacgaccga cgtgaagtcc agattcccta
     1921 cttcgaagtc cagatcgctg acttcttccc ggtgcctctc gatgttgcga tgagcccatc
     1981 gaccagagga ggaccgatga gtgcaccaac caccatgaac atcccccggt tccggggcga
     2041 catgatcgcc tccggcgacg atgcctacga cgttgctcgc gccgtctgga acggctccgt
     2101 cgaccgtcac cctcgactga tcgcccgttg tcgcggcacc gccgacgtgg ccgcagcggt
     2161 gaagttcgcc cgcgaccagg acctcgagat cgccattcgc ggcggtggtc acaacgtcgc
     2221 cggcaccgcg gtgtgcgacg gcgggatcgt gatcgacctg tcggcgatgc gcgctgtctc
     2281 ggtcgatccc gtcgagcgca cggctctcgt ccagggcggt gccctgtggg gcgacgtcga
     2341 tcacgagacg caggcccacg gcctcgcgac caccggcggc atcgtgggcc acaccggggt
     2401 cggcggcctc agcctgggcg gcggcatcgg ctggctcatg cgcaagcacg ggctcaccat
     2461 cgacaacctc gtggaggccg aggtggtcac cgcgcggggc gacatcatcc gggtatcagc
     2521 cgaccaacac acggacctgt tctgggcgct gcgaggcggc ggcgggaact tcggcgtcgt
     2581 cagttcgttc cggttcgcgc tgcacccggt cggtcccctg gtcatggccg gaccggtgtt
     2641 ctgggcggcc ga
//
LOCUS       DATDBZ010000528         4197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2634734, whole genome shotgun sequence.
ACCESSION   DATDBZ010000528 DATDBZ010000000
VERSION     DATDBZ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2634734"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VIX62_12230"
     CDS             complement(<1..815)
                     /locus_tag="VIX62_12230"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HEY5861045.1"
                     /translation="MTEIHVVHAQGEGFARGLHIGTELSAPIRDSVEFYHRYLDRRGV
                     SSEQLQDLLTPYLVGAETAYPDMMNVLKGMSVGAMVPILELFAINAFEELEPLLESPE
                     GELLFLQRKEGYVVPPITPPAVERCSSVSVNAGDVTMLAHNEHWLAGDVGNIAVIVDK
                     PDGGRVPVASPTIVCCLPAVGMNAHGVAQGIGSLTASDDGVGVPRVLVSRSSLEARDR
                     SDATARAAMPGRAGGYGHAYAFRGGDAFTLETSGKQHRVLDHPGPHTNHYQSDL"
     gene            complement(1032..2369)
                     /locus_tag="VIX62_12235"
     CDS             complement(1032..2369)
                     /locus_tag="VIX62_12235"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY5861046.1"
                     /translation="MSYRPLIAVVAYHLADDRVPRWPDGGYGVPAPYLEALRRAGART
                     AIIAPGEEGEPEALLEPFDGLVLVGGGDVHPSTYGADPDTEHLYGVERDRDVFEMGLV
                     HAADRLHVPTLCICRGMQIMNIAFGGTLHQHLPDMDGLLPHGVPVENTESLHPVTPEP
                     RTLLSATTKSRPLMCSSHHHQGVDRVGEDLVVSGRSPDGLVEAIEVPVPDMNTTGVSE
                     AEITWMLGVQWHPEETAATDPAQQSLFDSVTLLARHRGSRARPGERVGRNRAYGLSDY
                     DEGWPDRFAQEANRIVAAIPEGTVARIDHVGSTSVPGLAAKPIIDIQLSLVSMVPRSA
                     FVDPLVALGYDWTLDPWDDQHEYFSRSEGDDQVVHIHACEAGSDWERRHLAFRDVLRS
                     DPDAAAAYEALKHRLAADHPQDIMAYVDGKTGFVREIEARHLGTSPQEPARRG"
     gene            complement(2366..3094)
                     /locus_tag="VIX62_12240"
     CDS             complement(2366..3094)
                     /locus_tag="VIX62_12240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEY5861047.1"
                     /translation="MDAPQAKPILFVRCEPDETFGVGPAAVEAAGGSVMEWNALDTEA
                     PKPRLEDVAGVVVFGSSFNVEHADEQPFIKEVRELTREAVDTGVPYLGVCFGAQMLAW
                     SLDAEITKAPVREIGFEPIQPLATAAEDRLLAHYEPGDHVFQWHMDTFALPDGAELLV
                     TGDGVHHQAYRVGDRTWGVQFHFEIDRAEIETWLTAFEATEDLAVTWGKPSAAVRAEA
                     DRYLAVHQDKGRRVFERFAELSAP"
     gene            3163..>4197
                     /locus_tag="VIX62_12245"
     CDS             3163..>4197
                     /locus_tag="VIX62_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5861048.1"
                     /translation="MSAANPPADPADLLDLEHLLTDEERAIRDVAREWVRRRILPEVE
                     EWYEQGVFPARELGPELGGMGFLGMQLEGEHFLGLNSVAYGLVELELEAGDSGLRSFV
                     SVQSALSMYAIYAFGSDEQKAEWLPRMAAGEAIGCFGLSEPDSGSDPGSMRTVAKRDG
                     DDWVLSGTKMWITNGGVSDVAIVWANTDEGIRGFVVPTASPGFSAPDIHKKMSLRASV
                     TSELVMDGVRLPADAILPGVQGLKGPLSCLNEARYGIVWGVMGAARACFETALEYSKT
                     REQFGKPIGTFQLTQKKLADMALELNKGTLLALHLGRMKDEGRLQPEHVSFGKLNNTR
                     EALKITRTCRG"
BASE COUNT          640 a         1413 c         1470 g          674 t
ORIGIN      
        1 agatcgctct ggtagtggtt cgtgtgcgga ccggggtggt cgaggacccg gtgctgcttg
       61 ccgctggttt ccagcgtgaa cgcatcaccc ccgcggaacg cgtacgcgtg gccgtagccc
      121 cccgcgcgcc cgggcatggc cgcgcgcgcg gtcgcgtcgc tgcggtctcg cgcttcgagc
      181 gaactccggc tcaccagcac ccgggggacc cccacgccgt catcggaagc ggtgagcgaa
      241 ccgatcccct gcgcgacgcc gtgggcgttc atgccgaccg ccggcaggca gcacacgatg
      301 gtgggcgacg cgacgggcac ccgcccgcca tcgggcttgt ccacgatgac ggcgatgttg
      361 cccacatcgc cggcgagcca atgctcgttg tgcgccagca tcgtcacgtc tccggcgttc
      421 accgagacgc tcgaacagcg ttcgacggcc ggcggggtga tgggcggcac cacgtagcct
      481 tccttcctct gcaggaacaa cagctcccct tcgggtgact ccagcagcgg ctcgagctct
      541 tcgaaggcgt tgatggcgaa caactcgagg atcggcacca tggccccgac cgacatcccc
      601 ttcagcacgt tcatcatgtc ggggtaggcg gtttcggccc cgaccaggta gggcgtcagg
      661 agatcctgga gttgctcgga cgagaccccg cgacggtcga ggtagcgatg gtagaactcg
      721 accgaatcgc ggatcggcgc tgagagttcc gtgccgatgt ggagcccgcg cgcgaagcct
      781 tcgccctgcg cgtgcaccac atggatctcg gtcacgaacg gcccttcccc ggcgtcggaa
      841 cgcggacaag cgtacgcctc gggccgaagt ggcgggcgac gccggatgca gcgcgatgat
      901 tgcgcggcag cgttgttcgg catggcctga ctcggaccgt acggatgact cgtccctccc
      961 cgctcgagcc atgacttctt cggctcgccc tacgagatgc cggggattat ccaggtatcg
     1021 cccgggaaac gctagcccct tcttgccggt tcctgcgggc tagtccccag gtgccgggcc
     1081 tcgatctcac gcacgaaccc ggtcttgccg tccacgtagg ccatgatgtc ttgcgggtgg
     1141 tcggcggcca gccggtgctt gagcgcttcg tatgcagcgg cggcgtccgg atcggaacgg
     1201 agcacgtcgc ggaaggcgag gtggcgccgc tcccaatcgc tgccggcttc gcaggcgtgg
     1261 atgtgcacca cctggtcgtc accctccgag cggctgaagt attcgtgctg gtcgtcccac
     1321 gggtcgagcg tccagtcgta gccgagggcg acgagcgggt cgacgaaggc cgatcgcggc
     1381 accatcgaga ccaatgagag ctggatgtcg atgatcggct tggcggcgag ccctggcacc
     1441 gacgtcgaac ccacgtgatc gatcctggcc accgtccctt cggggatcgc ggcaacgatc
     1501 cgattcgcct cctgggcgaa tcgatccggc cacccttcgt cgtagtccga cagcccgtac
     1561 gcccggttcc ggccgacgcg ctcgccgggc ctggcccggg agccacgatg tcgggcgagc
     1621 agggtgaccg aatcgaacaa cgattgctgc gcgggatcgg tggccgctgt ctcctccgga
     1681 tgccactgca ccccgagcat ccacgtgatc tcggcctcgg acacgcccgt cgtgttcatg
     1741 tcgggcaccg gcacctcgat cgcttcgacc aggccgtcgg ggctgcgccc gctcacgacg
     1801 aggtcctcac cgacgcgatc gaccccttga tggtgatgcg acgagcacat gagtgggcgc
     1861 gatttcgtgg tggccgacag cagggtccgc ggctcgggcg tcaccgggtg cagggactcg
     1921 gtgttctcga ccgggacgcc gtgcgggagg aggccgtcca tgtcgggcag gtgctggtgc
     1981 agcgtgccgc cgaacgcgat gttcatgatc tgcatccccc ggcagatgca gagggtgggg
     2041 acgtgcagtc ggtcggcggc gtgcaccagt cccatctcga acacgtcccg gtcgcgttcg
     2101 acgccgtaga ggtgctcggt gtcgggatcg gctccgtacg tggaagggtg gacgtcgccg
     2161 ccgcccacca acaccaggcc gtcgaacggc tcgaggagtg cttcgggctc gccttcctcg
     2221 ccgggggcga tgatcgccgt acgcgctccc gcacggcgga gcgcctcgag gtagggcgcc
     2281 gggacgccgt agccgccgtc cggccaccgg ggcacccggt cgtcggcgag gtggtaggcc
     2341 acgaccgcga tcagcggccg gtagctcatg gggccgagag ctccgcgaat cgctcgaaca
     2401 cgcgccggcc tttgtcttga tggacggcga gataccgatc ggcttcagct cgcaccgcag
     2461 ccgatggctt gccccaggtg accgcgaggt cttcggtggc ctcgaacgcg gtgagccacg
     2521 tctcgatctc cgcccggtcg atctcgaagt ggaactgcac gccccaggtc cggtcgccga
     2581 cgcgatacgc ctgatgatgc acgccgtccc cggtgacgag cagctccgca ccgtcgggca
     2641 gggcgaacgt gtccatgtgc cattggaaga cgtggtcgcc gggttcgtag tgggccagca
     2701 gtcggtcctc ggcggcagtg gcgagcggtt ggatcggttc gaagccgatc tcccgtacgg
     2761 gcgccttcgt gatctcggcg tcgagcgacc acgccagcat ctgcgcgccg aagcagacgc
     2821 ccaggtacgg gaccccggtg tcgacggcct cgcgcgtgag ctcgcgcacc tccttgatga
     2881 agggctgctc atcggcgtgc tcgacgttga aggagctgcc gaacaccacc acgccggcga
     2941 cgtcctcgag gcgcggtttc ggagcctcgg tgtcgagcgc gttccactcc atgaccgagc
     3001 cgccggccgc ctcgacggcg gcgggaccca cgccgaacgt ctcgtcgggc tcgcaccgga
     3061 cgaacaggat gggcttggcc tgcggggcat ccatggcatc gagtgtggca cagcggccgg
     3121 gggtgccgca ccaccccgcg gccccggtgc taccgtcgaa ccatgagtgc tgccaacccg
     3181 cctgccgacc ccgccgacct gctcgatctc gagcacctgc tgaccgatga ggagcgcgcg
     3241 atccgcgacg tcgcccgcga gtgggtccgg cgccggatcc tgcccgaggt cgaggagtgg
     3301 tacgagcagg gcgtgttccc tgcgcgcgaa ctcggccccg aactcggcgg catgggcttc
     3361 ctcgggatgc agctcgaagg cgagcatttc ctgggcctga actcggtggc ctacggattg
     3421 gtcgagctcg aactggaagc cggcgattcg ggactgcgct cgttcgtctc ggtgcagagc
     3481 gcgctgtcga tgtacgccat ctatgccttc ggcagcgacg agcagaaggc ggagtggctc
     3541 ccgcgcatgg ccgccggcga ggcgatcggg tgcttcggcc tgtccgagcc cgactccggg
     3601 agcgaccccg gcagcatgcg gacggtggcc aagcgcgacg gcgacgactg ggtcctgtcc
     3661 ggcacgaaga tgtggatcac gaacggcggc gtgtccgatg tcgcgatcgt gtgggccaac
     3721 accgacgagg gcatccgggg gttcgtcgtg cccacggcct cgcccgggtt cagcgcgccg
     3781 gacatccaca agaagatgtc cctgcgcgcc tcggtgacgt ccgagctggt gatggacggc
     3841 gtgcggcttc ccgccgatgc gatcctgccg ggcgtccagg gattgaaggg cccgctgtcc
     3901 tgcctcaacg aggcccgcta cgggatcgtg tggggcgtga tgggcgcggc gcgcgcgtgc
     3961 ttcgagaccg ccttggagta ttcgaagacg cgcgagcagt tcggcaagcc gatcggcacg
     4021 ttccagctga cgcagaagaa gctggccgac atggcgctcg agctcaacaa gggaacgttg
     4081 ctggcgctgc acctcggtcg catgaaggac gagggccgtc tgcaacccga gcacgtgtcg
     4141 ttcggcaaac tcaacaacac gcgcgaggcg ttgaagatca cccggacctg ccgcggg
//
LOCUS       DATDBZ010000529         2262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2413878, whole genome shotgun sequence.
ACCESSION   DATDBZ010000529 DATDBZ010000000
VERSION     DATDBZ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2413878"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VIX62_12250"
     CDS             <1..729
                     /locus_tag="VIX62_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5861049.1"
                     /translation="LGSKVYMPRGDWPNQGRLSALHIRQACEDSLRRLQTDRIDLYQM
                     HHIDRNAWWDEIWEAMEQLKRTGKILYVGSSNFAGWHIARANEIAGYRHSLGLATEQS
                     LYNLTARTLELEVIPALVEYGMGLIPWSPLSGGLLAGALSKVEEGRRGTEYMQGEIEA
                     NRSKLEPWEALCAELGEDPAHVALAWLLHQPAVTAPIIGPRTEEQLLHAMHALEIKLG
                     PDVLTKLDEIFPGAGGPAPEAYAW"
     gene            complement(717..>2262)
                     /gene="mptB"
                     /locus_tag="VIX62_12255"
     CDS             complement(717..>2262)
                     /gene="mptB"
                     /locus_tag="VIX62_12255"
                     /inference="COORDINATES: protein motif:HMM:NF038066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HEY5861050.1"
                     /translation="SVDGWLTGRRAVAASIATFGTVALIAATPGSPFHPVLPETQGNG
                     PIGLLSQLLFLDELPHGVLIVIGFVAMIAAGVAFLLVLWAAERGEISTRTVVTLALVY
                     HGVVLLLPLLLSRDVFSYAFYGRILSQYGHNPYVSTPADFPANDLSRFVWPGWRDTPS
                     VYGPVFVWLAAAITAVFRGMVDVIEAFRFVAVAASLGSLWFVVRLVGRVRPQRQAYAA
                     AMIGLNPVVLFHTVGGGHVDVLVMLAVAAAIYLVATQRELPATVALTVGALVKISAAV
                     PLVLLIAYVIARAEPSRRWRVAGAHIGTALGIAFIAAVPFMQRSNPTLGMVELVQHGS
                     WIAPPALVERIFETVGTAVAGEIGGNAGVVLARLGMFAALAAGLFTIMRQVLRHAKEG
                     SVSFLAAAWGWSFLLMMLFSPTLFPWYFAWMLPVAWALPRVPRRTLEFSFIALVTAQL
                     TTENFRLPEWMHVDLAIGHPILVIVLVWFLRDLYLRLKHDVPLDAETEDVLLARAEPR
                     PPAYQA"
BASE COUNT          419 a          765 c          800 g          278 t
ORIGIN      
        1 ctgggatcga aagtctacat gcccaggggc gactggccga accagggccg cttgtccgcc
       61 ctgcacatcc ggcaggcttg cgaggactcg ctgcgtcggc tgcagaccga ccgtatcgac
      121 ctctaccaga tgcaccacat cgaccggaac gcatggtggg acgagatctg ggaagcgatg
      181 gagcagttga agcgcaccgg caagatcctc tacgtggggt cgtcgaactt cgccggatgg
      241 cacatcgctc gggccaacga gatcgccggc taccggcact cgttgggcct cgcaacggag
      301 cagtcgctct acaacctcac ggcgcgcacc ctcgagctcg aggtcatccc ggcgctcgtg
      361 gagtacggca tggggttgat cccgtggagc ccgctgtcgg gcggactgct ggccggcgcg
      421 ctctcgaagg tcgaggaagg tcgccgcggc accgagtaca tgcagggcga gatcgaggcg
      481 aaccgctcga agctcgagcc atgggaggcc ctgtgcgccg agctcggcga ggaccccgcc
      541 cacgtggccc tcgcatggct gctgcaccag ccggcggtga cggcgccgat catcgggcca
      601 cgcaccgagg agcaattgct gcacgcgatg cacgcgctgg agatcaagct cggccccgac
      661 gtgctcacga agctcgacga gatcttcccc ggagccggcg gtccggcgcc cgaagcctac
      721 gcctggtagg cggggggacg cggttccgcg cgggcgagca gcacgtcctc ggtctcggcg
      781 tcgagcggca cgtcgtgctt caggcgcagg tacaggtcgc gcaggaacca caccagcacg
      841 atcacgagga tcgggtgtcc gatcgccagg tcgacgtgca tccattcggg aagccggaag
      901 ttctcggtcg tgagctgggc cgtcaccagg gcgatgaacg agaactcgag cgtgcgccgc
      961 ggcacgcggg gcagcgccca cgcgaccggc agcatccacg cgaagtacca cgggaacaac
     1021 gtgggcgaga acagcatcat cagcaggaac gaccagcccc acgccgcggc caggaacgac
     1081 acggaccctt ccttcgcgtg gcgcagcacc tggcgcatga tcgtgaacaa cccggccgcg
     1141 agtgccgcga acatgccgag gcgagcgagc acgacgccgg cgttgccgcc gatctcgccg
     1201 gcgacggcgg tgcccacggt ctcgaagatg cgttccacca gggccggcgg ggcgatccac
     1261 gatccgtgct gcacgagttc gaccatgccg agggtggggt tcgaccgttg catgaagggc
     1321 acggcggcga tgaacgcgat gccgagggcg gtgccgatgt gggcgccggc cacacgccag
     1381 cgccgtgacg gttccgcgcg ggcgatgacg taggcgatca gcaggacgag cggcacggcc
     1441 gcggagatct tgacgagcgc ccccaccgtg agggccacgg tcgcgggcag ctcacgttgg
     1501 gtcgcgacca ggtagatggc tgccgccacc gcgagcatca ccagcacgtc gacgtggccc
     1561 ccgcccaccg tgtggaacag caccaccgga ttcagcccga tcatcgcagc ggcgtaggcc
     1621 tgccgctgcg gacgcacgcg gcccacgagc cggaccacga accagagcga acccaggctg
     1681 gcggccaccg ccacgaagcg gaaggcctcg atcacgtcga ccatgccgcg gaacacagcg
     1741 gtgatggccg ccgcgagcca cacgaacacc ggcccgtaga ccgacggcgt gtcgcgccac
     1801 ccaggccaca cgaaccgtga gagatcgttc gcggggaagt cggccggcgt gctcacgtac
     1861 gggttgtgcc cgtactggct caggatgcga ccgtagaagg cgtagctgaa cacgtctcgc
     1921 gagagcagca gcggcagcag cagcaccacg ccgtggtaga cgagcgcgag cgtgacgacc
     1981 gtgcgcgtgg agatctcgcc ccgctcggcc gcccacagca cgagcaggaa cgccacaccc
     2041 gcggcgatca tcgcgacgaa cccgatcacg atcagcacgc cgtggggcag ctcgtcgagg
     2101 aacagcagtt gcgagagcag tccgatgggt ccgttgccct gcgtctcggg gagcaccggg
     2161 tggaacggag acccgggggt ggcggcgatc agggcgacgg tgccgaacgt cgcgatcgat
     2221 gcggcgactg cccgacggcc ggtgagccat ccgtcgaccg ag
//
LOCUS       DATDBZ010000530         3482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_579219, whole genome shotgun sequence.
ACCESSION   DATDBZ010000530 DATDBZ010000000
VERSION     DATDBZ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579219"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VIX62_12260"
     CDS             complement(<1..385)
                     /locus_tag="VIX62_12260"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5861051.1"
                     /translation="MSTVWRCRDHDFPLGERTLVMGIVNATPDSFSDGGLFDVAADAV
                     KHAAQLAADGADILDVGGESTRPGADTVSVEEEIARVVPVIEGIRAEAPDTPVSVDTR
                     RGAVARAALDAGASIVNDITAGADPE"
     gene            438..1133
                     /locus_tag="VIX62_12265"
     CDS             438..1133
                     /locus_tag="VIX62_12265"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY5861052.1"
                     /translation="MTRHERTPRSRWRYVRGQGLEFDRVAFFTDAVFAIAMTLLIVSI
                     DAPKLPSPWEEPAVMLDALREQLPQFFSFFLAFLLIGRYWLAHHELFSSMKAVDRSFI
                     TLNLVYLAFVAFLPYPTELVGKYEANPISVVLFALCLAAISGMESVLLRLAYRHDLLR
                     IELGPTGYRYGMIQSTTPVVLFLVSIPIAFWNPTVALLTWLLAIPAGIWVDHRAGFRT
                     EQILAPPDPPDGT"
     gene            complement(1143..1715)
                     /gene="folE"
                     /locus_tag="VIX62_12270"
     CDS             complement(1143..1715)
                     /gene="folE"
                     /locus_tag="VIX62_12270"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY5861053.1"
                     /translation="MSGFDHEKIEQGVRLILEGVGEDTERGGLRGTPARVARMYEETL
                     AGIGNDPTGIVTVVEGADFDEMIMVRDISLTSLCEHHMLPFNGRAHVAYVPNKTGQIT
                     GLSKIARVVDVLAKRLQVQERLTTEIADTIEAALDPRGVFVVIEAEHLCMTMRGIKKP
                     GAVTVTSAVRGLFRTDARTRQEAMNHIGMR"
     gene            complement(1771..2343)
                     /locus_tag="VIX62_12275"
     CDS             complement(1771..2343)
                     /locus_tag="VIX62_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY5861054.1"
                     /translation="MQGAADPLAPGEDLYAVLDLRRDASEDDVRHAYRRLARTSHPDL
                     GGGAGAADRFRRITAAYEVLGNTERRASYDRDRRLRERGRGPAAAQHAPGPTGGTVRS
                     TPMPRTAERTQARPPEPATPLWSVDDWSAIRILGKLAVAAALVVTLAIGALVMVAVIR
                     EPDPLPAPTIFCRTPDGWLDCRRATQPGMP"
     gene            complement(2353..3015)
                     /locus_tag="VIX62_12280"
     CDS             complement(2353..3015)
                     /locus_tag="VIX62_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5861055.1"
                     /translation="MTDRWATFDCYGTLIDWFGGVRSQLVRLWPDADADALLATYLRA
                     EARLQEGKGTPYREVMAEALEAVGQEQGATVPVDERDALAASLPSWGVFPEVPGALRE
                     LRARGWKLAILSNTDPDLLDASLHAIDVPVDGRIVASEIGSYKPAFGHWETFFRMTGA
                     DRTRHVHVAASLFHDIEPCAKLGLPAVWINRRAETSMVPRSAELPDLTDLPDTLERLS
                     PA"
     gene            complement(3038..>3482)
                     /locus_tag="VIX62_12285"
     CDS             complement(3038..>3482)
                     /locus_tag="VIX62_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEY5861056.1"
                     /translation="AGNDIERATKIAHQMVTEYGMSDTIGPRTLGQKQGEVFLGRDWG
                     STPDYSDAVAFEIDSEIRQLIDEAHDVALDILTENRAKLDALAARLIEIETMDREEVE
                     QFFADIERRLPREHEVRSAGLAVSRQAKRPSTGKGSGGTLGLSPA"
BASE COUNT          484 a         1192 c         1227 g          579 t
ORIGIN      
        1 tctccgggtc ggcgcccgcg gtgatgtcgt tcacgatcga ggcgccggca tcgagcgcgg
       61 ctcgggccac ggccccgcga cgggtatcga cgctcaccgg cgtgtcgggt gcttcggcgc
      121 ggatgccctc gatcaccggc accacgcgag cgatctcctc ctcgaccgag accgtgtcgg
      181 cgcccggacg ggtcgattcg ccgcccacgt cgaggatgtc ggccccgtcc gccgcgagct
      241 gggcggcgtg cttcaccgcg tcggccgcga cgtcgaacag cccgccgtcg ctgaacgagt
      301 ccggcgtggc gttcacgatc cccatgacca gggttcgttc ccctaagggg aagtcgtggt
      361 cacggcagcg ccagacggtg ctcacacgaa ccatcgtagt cgcgcggtgc gcgggcggac
      421 cgtggcacaa tccgtcgatg acccgccacg aacgcacccc gaggtcacgg tggcgctacg
      481 tgcggggcca ggggctcgaa ttcgaccggg tggcgttctt caccgacgcg gtcttcgcga
      541 tcgcgatgac cctgctgatc gtctcgatcg acgcgccgaa actcccgtcg ccgtgggagg
      601 agccggcggt gatgctggac gcgctgcggg agcaactgcc gcagttcttc agcttcttcc
      661 tggcgttcct gttgatcggt cgctactggc tcgcgcacca cgagctgttc tcctcgatga
      721 aggcggtgga ccggtcgttc atcaccttga acctggtgta cctggccttc gtggcgttcc
      781 tgccctaccc aaccgagttg gtcggcaagt acgaagccaa cccgatctct gtggtgttgt
      841 tcgcgttgtg cctggcggcc atcagcggga tggaatcggt cctgctgcgg ctcgcgtacc
      901 ggcacgatct gctgcgtatc gagctcggac cgacgggcta tcgctacggg atgatccagt
      961 cgacgacacc ggtcgtgctg ttcctggtgt cgatcccgat cgcgttctgg aacccgaccg
     1021 tcgcgctgct aacgtggctc ctggcgatcc cggcggggat ctgggtggat catcgggcgg
     1081 ggttccgtac cgagcagatc cttgcgccgc cggatccgcc cgacggaacg tgacccgccg
     1141 tcctagcgca tgccgatgtg gttcatcgct tcctgacgcg tgcgtgcgtc ggtgcggaac
     1201 agccctcgca cggccgaggt gacggtgacg gcgccgggct tcttgatgcc gcgcatcgtc
     1261 atgcacaggt gctcggcctc gatcaccacg aacacgccgc ggggatcgag cgccgcctcg
     1321 atcgtgtcgg cgatctcggt ggtgagccgc tcctgcacct ggagccgctt cgccaggacg
     1381 tccaccacgc gcgcgatctt cgacagcccc gtgatctgcc cggtcttgtt gggcacgtag
     1441 gccacgtggg cgcggccgtt gaacggcagc atgtggtgtt cacacaacga ggtgagcgag
     1501 atgtcgcgca ccatgatcat ctcgtcgaag tcggcaccct ccacgacggt gacgatcccg
     1561 gtggggtcgt tgccgatccc ggcgagcgtc tcctcgtaca tccgagccac ccgggccggg
     1621 gttcctcgca gaccgccgcg ctccgtgtcc tcgcccacgc cctccaggat caggcggacg
     1681 ccctgctcga tcttctcgtg gtcgaacccg ctcatggtcg gatgctaccc ggtggacctg
     1741 ccgagccggg aacggatccg aacccggcgg tcacggcatg cccggctggg tggcgcgacg
     1801 gcagtcgagc cacccatcgg gggtccggca gaagatcgtg ggggcgggca acggatcggg
     1861 ttcccggatc acggcgacca tgaccagcgc gccgatcgcg agggtcacca ccagcgccgc
     1921 ggccaccgcg agcttgccca ggatccggat cgcgctccaa tcgtcgacgg accacagggg
     1981 cgtggccggt tccggtgggc gtgcctgtgt gcgttctgcc gtgcggggca tcggcgtcga
     2041 tcgcacggtc ccgccggtcg ggcccggcgc atgctgggcg gccgcaggac cacgcccgcg
     2101 ctcgcgcagt cgccgatccc ggtcgtagga ggctcggcgt tcggtgttgc cgagcacttc
     2161 gtaggctgcg gtgatccggc ggaaccgatc ggcggctccc gcacctcccc cgaggtcggg
     2221 gtgcgaggtg cgcgcgaggc gacggtaggc gtgccgcacg tcgtcctcgg aggcgtctcg
     2281 acgcaggtcg agcaccgcgt agaggtcctc acccggcgcg agagggtcgg cggcaccctg
     2341 catgcggcga gtctacgccg gtgacagacg ctcgagcgtg tcgggcaggt ccgtgaggtc
     2401 ggggagctcg gccgagcggg gcaccatgct ggtctcagca cgacggttga tccacacggc
     2461 gggcaggccg agcttggcgc acggctcgat gtcatggaag agcgaggccg ccacgtggac
     2521 gtggcgcgtg cgatcggctc ccgtcattcg gaagaacgtc tcccagtgcc cgaacgccgg
     2581 cttgtaggaa ccgatctcgg acgccacgat ccgtccgtcg accggcacgt cgatcgcgtg
     2641 gagcgaggca tcgagcaggt cggggtcggt gttggacagg atcgcgagct tccacccgcg
     2701 cgctcgcagc tcccgcagcg cgccgggcac ctcggggaac acgccccagg acgggagcga
     2761 ggccgccagg gcgtcccgtt cgtccacggg caccgtcgcg ccctgctcct ggccgacggc
     2821 ctccagcgcc tcggccatca cctcccggta gggcgtgccc ttgccctcct ggagccgggc
     2881 ctcggcacgc aggtaggtgg ccaagagcgc gtcggcgtcg gcgtcgggcc acaggcgcac
     2941 gagctgcgaa cgcacaccac cgaaccagtc gatgagggtg ccgtagcagt cgaaggtggc
     3001 ccagcggtcg gtcacggtgc gcagtctacc gagcggctca ggcggggctg aggccgagcg
     3061 tgccgccgga gcccttgccc gtggacggtc gtttggcctg ccgggagacc gcgagcccgg
     3121 ccgagcgcac ctcgtgctcc cgcggcaacc ggcgctcgat gtcggcgaag aactgctcga
     3181 cctcctcgcg atccatcgtc tcgatctcga tgagtcgagc ggcgagcgca tcgagcttgg
     3241 ctcggttctc ggtgaggatg tcgagggcca cgtcgtgcgc ctcgtcgatc agctgccgga
     3301 tctccgagtc gatctcgaag gcgacggcgt cggagtagtc gggtgtggat ccccagtcgc
     3361 ggccgaggaa cacctcgccc tgcttctggc cgagcgtgcg gggaccgatc gtgtcgctca
     3421 tgccgtactc ggtgaccatc tggtgagcga tcttcgtggc ccgctcgatg tcattgccgg
     3481 ct
//
LOCUS       DATDBZ010000531         2332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1624575, whole genome shotgun sequence.
ACCESSION   DATDBZ010000531 DATDBZ010000000
VERSION     DATDBZ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624575"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VIX62_12290"
     CDS             complement(<1..450)
                     /locus_tag="VIX62_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEY5861057.1"
                     /translation="MTYDPRQPDPRNTPARPTLTPPPFPSALAQPRISQGAPIAPAPT
                     SPAAVPVPPKAGRTAPVWIVGVIVLILVGLVGYFLSAIGPAASIIGMLLAFVPLAAVL
                     FAIRMIDRWEPEPRGLLVLAVAWGAVAAVGITLGVDLLVSMVLGVDDS"
     gene            515..1381
                     /locus_tag="VIX62_12295"
     CDS             515..1381
                     /locus_tag="VIX62_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5861058.1"
                     /translation="MRFAHVSLPGFEEPRLAVVDGDTALIVAELFPGAPRYLEELIAG
                     GDELLDRVRDAAAGAGPWHPLSEATYASALLTPPVILAIGLNYAAHSSELGLKTDSTP
                     TVFVLWPNSLAGHDATTTWPRVLSESVDYEAELGVIIGAPAKDVSPEEALSHVWGYTV
                     VNDITARDIQYSEAQWSRCKSFDGFTPTGPFVVTADEVPDPQDLHIWTVLDGHTLQDA
                     STNQMVRPVATLISHLSKSATLLPGTLISTGSPGGAGYSRDPQVFLRDRSTVTVGIDG
                     IGALTTHCRILD"
     gene            complement(1433..1882)
                     /locus_tag="VIX62_12300"
     CDS             complement(1433..1882)
                     /locus_tag="VIX62_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEY5861059.1"
                     /translation="MTDPQNPDVPPPPSAYNSPPPPAYDAAPTYSAAPPAPYGAPANA
                     PVPGRTLGIVALIVAIFFNVIGLILGIVALVQSRKAGHKNGFAVAAIIVGAVLTVLGI
                     IVGILVFTAFAAAAGFAGEVLQACQAVDFTGTVTVQGQSVDCSTIAR"
     gene            complement(1965..>2332)
                     /gene="aspA"
                     /locus_tag="VIX62_12305"
     CDS             complement(1965..>2332)
                     /gene="aspA"
                     /locus_tag="VIX62_12305"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013583442.1"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEY5861060.1"
                     /translation="EPVIAHSLFQSITWMRRGMHTLRVNCVDGITANTERLGAMVGAS
                     VGVITALTPFIGYAAAAALAKTALLTGRNVADLVVEAGLMSREECMKQLSPARLSGLE
                     TVTAAIPVVYADDSGDTEN"
BASE COUNT          380 a          793 c          822 g          337 t
ORIGIN      
        1 cgaatcgtcc acacccagga ccatgctcac gagcaggtcg acgccgaggg tgatgccgac
       61 ggcggccacc gcaccccacg cgacggccag gacgagcagc ccacggggct cgggctccca
      121 gcggtcgatc atgcggatcg cgaacagcac ggccgccaac ggcacgaagg cgagcagcat
      181 cccgatgatc gatgcggcgg gcccgatcgc gctcaggaag tatccgacga gtccgacgag
      241 gatcagcacg atcaccccga cgatccagac gggggcggtg cggcccgcct tcggaggcac
      301 cggaacggcc gcgggcgagg tcggcgcggg cgcgatcgga gcgccctgcg agatccgcgg
      361 ctgcgcgagg gccgagggga agggcggagg tgtgagggtc ggtcgggcgg gtgtgttccg
      421 ggggtcgggc tgcctcgggt catacgtcat gcgcacagca tatggctgcg ccgaacgcac
      481 cgcgcagggt atggcggccc ggtagcgtgg aaccatgcgc ttcgcccacg tgtccctgcc
      541 ggggttcgaa gagccccgcc tcgcggtggt ggacggggac acggcgctga tcgtcgcgga
      601 gctgttcccc ggagcccccc gatacctcga ggagctgatc gccggtggcg acgagctgct
      661 cgaccgggta cgtgatgccg cggcgggtgc cgggccgtgg catccgctct ccgaagcgac
      721 gtacgcgtcc gcgctgctga ctcccccggt gatcctggcg atcggtctga actacgccgc
      781 gcactccagc gagctggggt tgaagacgga ttccaccccg acggtgttcg tcctgtggcc
      841 caactccctg gccgggcacg acgccacgac cacctggccc cgcgtgctca gcgagtcggt
      901 ggactacgag gccgagctcg gcgtcatcat cggcgcgccg gcgaaggacg tctcacccga
      961 ggaggccctg tcgcacgtgt ggggatacac cgtggtgaac gacatcacgg ctcgcgacat
     1021 ccagtactcc gaggcgcagt ggtcgcgctg caagtcgttc gacgggttca ccccgaccgg
     1081 gccgttcgtg gtcaccgccg acgaggtgcc cgatccgcag gatctgcaca tctggacggt
     1141 cctggacggg cacacgctgc aggacgcctc gaccaaccag atggtacggc cggtcgcgac
     1201 tctgatctcg cacctgtcga agtccgcgac tctgctgccc ggcacgctca tctcgacggg
     1261 cagccccggc ggtgccggct actcccgcga cccgcaggtg ttcctgcggg accgctcgac
     1321 cgtgaccgtc gggatcgacg gtatcggcgc cctcacgacc cactgccgca tcctcgattg
     1381 atcacagcgc gaacgcccgc caccgaggtg acgggcgttc gtgcatgatc gatcagcgtg
     1441 cgatggtgct gcagtccacg ctttgaccct ggacggtcac cgtgccggtg aagtcgaccg
     1501 cctgacacgc ctgcaatacc tcaccggcga agccggccgc cgcagcgaac gcggtgaaca
     1561 ccaagatccc gacgatgatc ccgaggacgg tgaggaccgc gccgacgatg atcgccgcga
     1621 ccgcgaaccc gttcttgtgc cccgccttgc ggctctggac gagcgcgacg atgcccagga
     1681 tcagaccgat gacattgaag aagatcgcca cgatgagcgc gacgatgccg agcgtcctgc
     1741 ccggaacagg cgcgttggcg ggcgcaccgt acggcgcggg cggcgccgcc gagtatgtcg
     1801 gggccgcatc gtatgccggc ggcggcggcg agttgtacgc ggacgggggc ggcggcacgt
     1861 cggggttctg cggatcggtc attgcaaggt cctctcgatg gaagctgtgc ttcgaacgct
     1921 agcgcccgtg cgcgaccggc ggcaacgacc gacacaccac ccggtcagtt ctccgtgtcg
     1981 cccgaatcgt cggcgtacac gaccggtatc gccgctgtga cggtctccag acccgaaagg
     2041 cgggccggcg acagctgctt catgcactct tcccgtgaca tgagcccggc ttcgaccacg
     2101 aggtctgcga cgttgcgtcc ggtcaggagg gcggtcttgg cgagggccgc agccgcggcg
     2161 tagccgatga agggggtgag agccgtgatg accccgaccg acgcaccgac catcgcaccg
     2221 aggcgctccg tgttggcggt gatgccgtcg acgcagttga cgcgcagtgt gtgcattccc
     2281 cggcgcatcc aggtgatcga ctggaacagc gagtgcgcga tgaccggctc ga
//
LOCUS       DATDBZ010000532         2796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2834155, whole genome shotgun sequence.
ACCESSION   DATDBZ010000532 DATDBZ010000000
VERSION     DATDBZ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2834155"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..874)
                     /locus_tag="VIX62_12310"
     CDS             complement(212..874)
                     /locus_tag="VIX62_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY5861061.1"
                     /translation="MSRKKLYLLGPLAVAAAIALSACSPMSSGSTTTEETAAPPAAEE
                     TMAPETEMDPAANLVGAGCAGYAEAVPEGEGSVAGMAEDPVATAASNNPILTTLTAAV
                     SGGVNPDVNLVDTLNGDEYTVFAPVDDAFAKIDPATIEVLKTDAALLTSILTYHVVPG
                     QVLPADIEGTHTTVQGADLEVTGSGDDIMVNGEAAVICGGVQTANATVYLIDTVLMPP
                     AQ"
     gene            1072..1674
                     /gene="sigK"
                     /locus_tag="VIX62_12315"
     CDS             1072..1674
                     /gene="sigK"
                     /locus_tag="VIX62_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016464474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF RNA polymerase sigma factor SigK"
                     /protein_id="HEY5861062.1"
                     /translation="MLDDVVIDGVDVPEDGADAVDHAGQLLLRVADGDQAAFARLYDM
                     LSPRAFGLILRVLVDRSQSEEVLQEVFLEIWQSAARFAPNKGQGRSWVLTIAHRRAVD
                     RVRSAQASTDRDVRAGFRDMDVAHDGVAEEVELRIEGQRVSEALATLPDPQREAITLA
                     YFGGYSQSEIAALVGAPLGTIKTRMRDGLSRLRAEMGVTS"
     gene            1671..2423
                     /locus_tag="VIX62_12320"
     CDS             1671..2423
                     /locus_tag="VIX62_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY5861063.1"
                     /translation="MNEQEFAELAAGHALYALSPDDERAFENARRQHPEWEHLVTADA
                     ETAAVLAESVSDVAPPPGLRSALLAQIAGDEQAPTAQPAEEAENTPTPEPVKPRRWGA
                     RSWFALAASLALLVGVGWGAVTVGERLNTPASVVALDQIESASDAQSETVTLSDGGEV
                     TAHWSESVGKAVLVSDGLPTIADDESFELWFVRDGAAIPAGTFTSEDGTATALLEGGI
                     ESGDVIAVTVEQAGGSPTGQPTSDPIVQIETA"
     gene            2518..>2796
                     /locus_tag="VIX62_12325"
     CDS             2518..>2796
                     /locus_tag="VIX62_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY5861064.1"
                     /translation="MSDDSREFHKPVRRPAELFDRRFAAEDPAEVSRVAHSTAHALLA
                     RAREDPDGEVVDRLVAFTAEHGIDDIAELWSRSPSRSLPGTLWRLYLIQ"
BASE COUNT          437 a          920 c          976 g          463 t
ORIGIN      
        1 tccgatgctc taccgactga gctagaggcc ctccagatcc agcctacttc gcggtggcgg
       61 accggccggc agcttccacg ctcgtgaacg ggaaaggacg ccaccgaagt gacgtccgtc
      121 cctcccaatc agcacgaccg ccgacgccac ctcgcaggcg tcggcggtcg caccgccccc
      181 aggaatcccg gcttgcgccg ggagactgga attactgagc cggcggcatc agcaccgtgt
      241 cgatgaggta cacggttgcg ttggccgtct ggacgccacc gcagatgacc gcggcctctc
      301 cgttgaccat gatgtcgtct ccgctgccgg tgacctcgag gtcggcgccc tgcaccgtgg
      361 tgtgggtgcc ttcgatgtct gcaggaagaa cctggccggg aaccacgtgg tacgtcagga
      421 tcgaggtcag cagcgcggcg tcggtcttga gcacctcgat cgtggcgggg tcgatcttcg
      481 cgaacgcgtc atcgaccggc gcgaagacgg tgtactcgtc gccgttgagc gtgtcgacca
      541 ggttgacgtc cgggttcact ccacccgaga ccgcggcggt cagggtggtg aggatcgggt
      601 tgttcgacgc ggcggtggcc accgggtcct ccgccatgcc ggcaaccgag ccttcgccct
      661 ccgggacagc ctcggcgtaa ccggcgcagc cggcaccgac gaggttggcc gcggggtcca
      721 tctccgtctc gggcgccatc gtctcttcgg cggccggcgg ggcggcggtc tcttcggtcg
      781 tggtcgagcc gctcgacatg ggcgaacatg cgctcagtgc gatggctgcg gcgactgcca
      841 ggggtccgag cagatacagc ttcttgcggg acatcttgtc ctcctaggtt tcgcgactcc
      901 ggggcggtgt cgcagtgcca gtggtgctgt ggccactcga agcggcctac acggtgtctt
      961 cggcgccctc gtcggatcgg atgggacatt cctcgaaaca gttcggatgc cgcatcacga
     1021 tgcgactgtc aaccatcgcg tcgcgtgcgc tcgcgcgcgc ggtactaggg catgctggat
     1081 gacgtggtga tcgatggcgt ggacgtgccc gaggacgggg cggacgcggt cgaccatgcc
     1141 ggccagttgc tgctccgcgt cgcggacggc gatcaggccg cattcgcccg cctctacgac
     1201 atgctctcgc cccgggcgtt cggcctgatc ctgcgggttc tggtcgatcg gtcgcagagc
     1261 gaggaggtcc ttcaggaggt gttcctcgag atctggcaat ccgccgcgcg attcgctccg
     1321 aacaagggtc agggaagatc gtgggtactc acaatcgcac atcgccgggc ggtggaccgc
     1381 gtgcggtccg cacaggcgag taccgatcgt gatgtgcggg cgggcttccg tgacatggac
     1441 gtcgcccatg acggcgtcgc tgaagaagtg gaactgcgga tcgaagggca gcgcgtctcc
     1501 gaggcgctcg caactctgcc cgatccgcag cgggaagcaa tcacccttgc gtatttcggg
     1561 ggttacagtc agagcgagat cgcggcactc gtcggagctc cgctgggcac gatcaagacg
     1621 aggatgcggg acggcttgtc ccgcctccgg gcggagatgg gggtgacatc gtgaacgagc
     1681 aggagttcgc cgaactggcg gccggtcacg cactgtacgc cctgtcaccc gatgacgaga
     1741 gagctttcga gaacgcgcgt cgacagcatc cggagtggga gcatctcgtg acagcggatg
     1801 ccgaaacagc cgcggtgctg gccgagagcg tctccgacgt ggcgccgccc cccggtcttc
     1861 gcagcgcact gctcgcacag atcgccggtg acgagcaggc acccaccgcg cagccggcgg
     1921 aagaggccga gaacactccg acaccagagc ccgtcaagcc ccggcgctgg ggcgcgcggt
     1981 cctggttcgc cctcgcggca tcgctcgccc tcctggtggg agtcgggtgg ggtgcggtca
     2041 ccgtcggcga acgtctcaac acgccggcct ccgtcgtggc cctcgaccag atcgagtcgg
     2101 cgtccgatgc ccagtctgag acggtcaccc tctcggacgg cggcgaggtc accgcccact
     2161 ggtcggaatc ggtcggcaag gccgtgctcg tctcggacgg actgccgacg atcgcggacg
     2221 atgagagttt cgagctgtgg ttcgtccgcg atggggcggc gattccggcg ggcacgttca
     2281 cgagcgagga cggcaccgcg accgccctgc tcgagggagg gatcgagtcc ggcgacgtca
     2341 tcgcggtcac ggtggagcag gccggcgggt cgccgacggg tcagccgacg tccgatccga
     2401 tagtccagat cgagaccgcc tgagccgaac tgcccacgtc tcgatgtgga tgctgcaacg
     2461 cacccggagg cggcgcggaa cggtctcgag gcgacgttcc gcgggtagcc tgaagtggtg
     2521 agcgacgatt cgagggagtt ccacaaacct gtccgccggc cagccgagct cttcgaccgg
     2581 cgcttcgcgg cggaagatcc cgccgaagtc tcgcgtgtcg cccactccac cgcacacgct
     2641 ctgctcgctc gcgctcggga ggaccccgat ggcgaggtcg tcgaccgatt ggtcgccttc
     2701 acggccgagc acggaatcga cgacatcgcc gaactctggt cgcgttcgcc gtcccgatcg
     2761 ctgcccggca cactgtggcg gctgtacctg atccag
//
LOCUS       DATDBZ010000533         2379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2636884, whole genome shotgun sequence.
ACCESSION   DATDBZ010000533 DATDBZ010000000
VERSION     DATDBZ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2636884"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VIX62_12330"
     CDS             <1..418
                     /locus_tag="VIX62_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEY5861065.1"
                     /translation="ISYDPTNHGRMVALRAAKVDGIAASLPPIEVVGDVDDAELLVLG
                     WGSTWGAITSAVKRARAAGDKVAHAHLRHLHPFPRDLGEILARYPRVLVPEMNLGQLS
                     KLVRAEFLVDARSVTKVEGTPFTAAELHVAIEEALR"
     gene            415..1431
                     /locus_tag="VIX62_12335"
     CDS             415..1431
                     /locus_tag="VIX62_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY5861066.1"
                     /translation="MTDLQPTTKKDWSSDQEVRWCPGCGDYSILTAVQMLMPDLAVRR
                     EDTVFISGIGCAARFPYYMNTYGMHSIHGRAPAIATGLALSRPDLDVWVIGGDGDLLS
                     IGGNHLIHALRRNVDLTILLFNNQIYGLTKGQYSPTSEVGKVTKSTPFGSLDTPFNPV
                     ALALGAQASFVARTHDMDRKHMQETFRRAHAHKGAALVEIYQNCNVFNDGAFDTITSK
                     GTRDSMLIPLEHGQPVRFGAEDEKGVVIGDDGRAAVVAVADVGEDALVVHDERSPDAS
                     LAFTLAQLAPGPDVPTPVGVFRAVERPEYAEEMTRQLAAAQDDQGPGDLAALLHSGAT
                     WTVI"
     gene            complement(1444..>2379)
                     /locus_tag="VIX62_12340"
     CDS             complement(1444..>2379)
                     /locus_tag="VIX62_12340"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY5861067.1"
                     /translation="MAFTDAAGRHRHTTGVIGALTLSRPDEGFILPHEHTTPKAKSDR
                     LQMLRFCRANLSAIWVLTPAPVTELIGSPADGDPVWTDEDGTEHALWVIDDPDTVAAL
                     SGAVASRPVVVADGHHRYETSLAYRDERRGAADGTPGSAGPAEATMAYVVELVAAELT
                     VWPTHRVLEDLDPGLDVAAALAPWFELGDATTLDALTLDTMVDTGTLVLVLPDGAWAM
                     RPRPEAFDGVRDLDTSRIEVALADLGDATVRYHHDIAEVQRAVVSGEAQAGILVRPAT
                     VDAIAQTADGGERMPPKTTFFHPKPRTGPVIRLLD"
BASE COUNT          379 a          805 c          815 g          380 t
ORIGIN      
        1 catctcctac gacccgacga accacgggcg catggtcgcg ctgcgtgcgg cgaaggtcga
       61 tggaatcgcc gcatcccttc cccccatcga ggtcgtcggc gatgtcgacg acgccgagct
      121 cctcgtgctc ggctggggat cgacatgggg tgccatcacc tcggcagtga agagagcgcg
      181 agcagcgggc gacaaggtgg cccacgcgca tctacgccat ctccacccgt tcccgaggga
      241 cctcggcgag atcctcgccc gatacccgcg tgtcctggtg cccgagatga acctgggcca
      301 gctgtccaag ctggtgcgtg ccgagttcct ggtcgacgcc cgatcggtca ccaaggtcga
      361 aggcacgcct ttcactgcgg ccgaactgca cgttgcgatc gaggaggccc tgcgatgacc
      421 gaccttcagc ccaccaccaa gaaggactgg tcgagcgacc aggaggtccg gtggtgtccc
      481 ggttgtggcg actactcgat cctgacggct gttcagatgc tgatgcccga cctcgccgtg
      541 cgccgcgagg acaccgtctt catctcgggg atcggttgcg ccgcacgctt cccgtactac
      601 atgaacacct acgggatgca ctcgatccac ggccgggcgc cggcgatcgc cactgggctc
      661 gccctgtctc gtccagacct cgacgtgtgg gtcatcggcg gcgatggcga cctgctgtcg
      721 atcggcggca accatctgat ccacgcgctg cgccgcaacg tggacctcac catcctgttg
      781 ttcaacaacc agatctatgg gctgaccaag ggtcagtact caccgaccag cgaggtgggc
      841 aaggtcacga agtcgacgcc gttcgggtcg ctcgacacgc cgttcaaccc tgtcgccctg
      901 gcgctcgggg cccaggccag cttcgtggcg cgcacccacg acatggaccg caagcacatg
      961 caggagacct tccggcgggc gcacgcccac aagggcgcag cgctggtcga gatctatcag
     1021 aactgcaacg tgttcaacga tggagcgttc gacaccatca cgtccaaggg cactcgcgac
     1081 tcgatgctca ttccgctcga acacggtcag cctgtccgct tcggggccga ggacgagaag
     1141 ggcgtggtga tcggcgatga cggccgggca gcggtcgtcg cggtcgccga cgtgggagag
     1201 gacgcgctcg tcgtacacga cgagcgttcg cctgacgcgt cgctcgcctt caccctggcc
     1261 cagctcgcgc cggggcccga cgtacccaca ccggtcggag tgttccgggc ggtcgagcgt
     1321 cccgagtacg cggaggagat gacgcgccaa cttgccgctg cccaggacga tcagggtccc
     1381 ggcgacctgg ccgctctgct gcactcggga gcgacctgga cggtcatctg acaggcgcca
     1441 tgatcagtcc aggaggcgga tcaccggtcc ggtgcgcggc ttcggatgga agaaggtcgt
     1501 cttgggtggc atgcgttccc ctccgtccgc ggtctgggcg atcgcgtcca ccgtcgcggg
     1561 ccgcacgagg atgcccgcct gagcctcgcc gctcacgaca gcacgctgca cctcggcgat
     1621 gtcgtggtgg tagcgcaccg tggcatcgcc aaggtcggcg agcgccacct cgatgcggct
     1681 ggtgtcgagg tcacgaaccc cgtcgaaggc ctccggacgc gggcgcatcg cccatgcgcc
     1741 gtcggggagg acgaggacga gcgtgccggt gtccaccatc gtgtcgagcg tgagcgcgtc
     1801 gagggtcgtc gcgtctccga gctcgaacca gggcgccagt gccgcggcga cgtcgagccc
     1861 cggatcgagg tcctccagga cccgatgggt gggccagacc gtgagctcgg cggcgacgag
     1921 ctcgacgacg taggccatcg tcgcctccgc gggacccgct gatccgggtg tcccgtcggc
     1981 cgccccgcga cgttcgtccc ggtaggcgag cgaggtctcg taccggtgat ggccgtcggc
     2041 gaccaccacg ggacggctgg cgaccgcgcc agacaaggcg gcgacggtgt ctggatcgtc
     2101 gatgacccac agcgcgtgct cggttccgtc ctcgtctgtc cacaccgggt caccatccgc
     2161 tggcgagccg atcagttcgg tgacgggcgc gggtgtgagt acccagatgg ccgagaggtt
     2221 ggcccgacag aaccgcagca tctggaggcg atcgctcttg gccttcgggg tggtgtgctc
     2281 gtgagggagg atgaagccct cgtcgggcct ggacaaggtg agcgcgccga tgacccctgt
     2341 cgtgtgccgg tggcgaccag cggcgtcggt gaacgccat
//
LOCUS       DATDBZ010000534         9045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_580049, whole genome shotgun sequence.
ACCESSION   DATDBZ010000534 DATDBZ010000000
VERSION     DATDBZ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9045
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580049"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="ccsB"
                     /locus_tag="VIX62_12345"
     CDS             <1..889
                     /gene="ccsB"
                     /locus_tag="VIX62_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY5861068.1"
                     /translation="MVGYFVFLAFKREPIWQVSRAVAVVGVAANLVSIVARGLAADWV
                     PWGNMYEYSVMLAMLVVAGYLIIVEGMYKVRTLGGFALMFSVLTMAVAVSFLYVGPGP
                     LVPALNSYWIKIHVQAAIWGSSLFALGGIVTILYLVQDWRERRQVEALRGSGPPPIMG
                     GALDLDLNAPDDLDRDVDEVPVAAAAPRASRLPSAQTLDRVAYRIIAFAFPIWTFAVI
                     AGAIWAQEAWGRYWGWDPKETWSFITWTIFAGYLHARATSGWKGRRAAVIALVGFVSL
                     LVTYYVVNLWIVGLHSYAK"
     gene            987..2081
                     /gene="coxB"
                     /locus_tag="VIX62_12350"
     CDS             987..2081
                     /gene="coxB"
                     /locus_tag="VIX62_12350"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY5861069.1"
                     /translation="MRKKVLWGVGLAALALLLAACAPDATQSTLKPVGEPAIEQKRLF
                     IAVIWIAAAVFVIVEGGILFLVIRYRHRKGRERMPKQTHGNTRLEIGWTIAPALVLAV
                     VMVPTISMIWDLARPPSPDALNITVQGYQWWWGFQYSDSDMTVGSGEQRPIEIADVMV
                     VPTGREIYLSLEAEGGGAKTTDGTPDFQVIHSFWVPELFGKQDVVPGHTNHITFQVDE
                     AGTYTGQCAEFCGLQHGRMKLRVVALDPDDWELWVVNQQQGPALVSDPLAKQGEEIFM
                     NPLSGGRGACTACHAVGDVGAAAAPNLSHFNEPTHECFAGCNWETSDVDALKAWLRDP
                     DAVKMGAKMPNYQLSDDEIDALVAYLYSLT"
     gene            2110..3978
                     /gene="ctaD"
                     /locus_tag="VIX62_12355"
     CDS             2110..3978
                     /gene="ctaD"
                     /locus_tag="VIX62_12355"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY5861070.1"
                     /translation="MSVTGTSSRGLRRPSSLTGFWSWFTTVDHKKIGIMYGVSAFVFF
                     IVGGLEALLIRAQLAQPDGTLLNADQYNQIFTMHGITMIFLFVMPMSAAFFNYFLPLM
                     LGARDVAFPRLNAYSFWVFLLGGLFLYSSPFLGGAPNGGWFGYAPNSTTDPSIGMSFY
                     ALGLLITGIASAAGAVNLAITIINMRAPGMSLFRMPVFVWMGLVVQFLLVFALPIITV
                     GLFELLFDRRWGTHFFDPTAGGDPILWQHLFWLFGHPEVYILILPAFGIISEIIPVFA
                     RKPLFGYQFMVFSGIAIGFIGFGVWAHHMFAAGLGPVANTAFGVSTAIIAIPTGVKIF
                     NWMATLHGGDLRFTSPMLYAIGAVTMFVIGGLSGVTHAVVPSDYQQTDTYYVVAHFHY
                     VIFGGSVFAFFGAFHYWWPKVFARQLSEALGKVAFWILLIGFNLTFGPMHILGLSGMI
                     RREYSYPESLGLTFWNQIATLGAFLIALSVLTFMVNVVRTHIRKQPLASEDPWDARTL
                     EWMTACPPPVYNFEEIPQVHSLDEFWHRKYVEDEKTGTLVPAQAGAAPEHEEDHAEAG
                     HGIHLPSPSYWPLVSAVGLPVIAYGVLYSWWLVAAGALVVLVGFMGWAVEPSVAED"
     gene            3981..4610
                     /locus_tag="VIX62_12360"
     CDS             3981..4610
                     /locus_tag="VIX62_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY5861071.1"
                     /translation="MAMAIEKPHAGEGGEHETTTGLPNTKLAMWLFLASECLLFGALI
                     STYVLYRGASTVGPYPADVFDIPYTSVSSFVLLASSLTMVLALTAAQKRDVPRMRLWL
                     LATALLGLTFVGGQVYEFTAFYHEGLALSTNLFGTTFYVLTGFHGVHVTVGIFMLLSL
                     VGLSGADRLPDDAGFPVEMVGLYWHFVDVVWIIIFTVVYLIPTGPTPAN"
     gene            4620..4979
                     /locus_tag="VIX62_12365"
     CDS             4620..4979
                     /locus_tag="VIX62_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY5861072.1"
                     /translation="MADTETPFQQELGQEDLADPRESQAVHREHPSAKEYIRIGIILA
                     VLTALEIAASYADVRPAILIPTLYALAFVKFALVVLWFMHLKFDDRRYARFFVMGIAL
                     AGTLYLIVLITFKVFLR"
     gene            4992..5819
                     /locus_tag="VIX62_12370"
     CDS             4992..5819
                     /locus_tag="VIX62_12370"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY5861073.1"
                     /translation="MLPAWHVHPDVWLLFGSIIAAYLIAGRRHERATGEATEPRKRRL
                     FLLGMGVLWLGADWPIHDLAERYLYSMHMTQHLLFTLVAAPILLAGMPVWLLRELLAP
                     APIRRIFRFMTRPLVALIFFNAVLLFTHWPDVVAASVGSELVHLGLHVLIVTSALVMW
                     WPVMSPLPEMPSLPPPGQMLYLFLQSLAPTIPASFLTFGHKPLYPVYATFPRIWGIDA
                     LTDQLIAGLIMKLLGGLLLWGVIAVIFFRWYNQEQRDGLDPTTHQNIRREIGAELSR"
     gene            5816..6760
                     /locus_tag="VIX62_12375"
     CDS             5816..6760
                     /locus_tag="VIX62_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY5861074.1"
                     /translation="MSGRENRQSLLLPILLPLGILGVIALVLYGFSRILLNITSTAAT
                     VLACIAAVAVVALATFVASRPRVGASSLFSVVGGVAGVAMLAGGLALLVAPPAEEGED
                     GGTEGPVVTLAAPVGAAADGFSTNKLSVPAEEAFTIDFDNEETGVQHNVVIFDGKDAE
                     APALFTGELIIGPKTTPYSVDALPEGNYFFHCEIHPTTMTGDVIAKPGTGGEPSGDEG
                     GGAGSPTIAAQGTAFNTSELAFKAETATPLVFDNQDDAAVTGPHNVSIYDGDAALFTG
                     DLINGPQQVTYDIPALKPGEYQFRCDVHPEMAGAVTVT"
     gene            6812..7363
                     /locus_tag="VIX62_12380"
     CDS             6812..7363
                     /locus_tag="VIX62_12380"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5861075.1"
                     /translation="MRKVAVIVVAALVVSLLAAFVFRPRGGDPRIEGGVVEMDQPIPD
                     LTAIDTVSGDPLPPDAFEGSATVINVWANWCAPCRQEQPALVATQARYADQDVRFVGI
                     NYNDDLELARRWVDDFEVTYPSLYDPQGRTAALLDFPFLPDTYVVDGAGTIRYAIYGE
                     TDEDELSGLIDDVLAGDTASSGT"
     gene            complement(7360..>9045)
                     /locus_tag="VIX62_12385"
     CDS             complement(7360..>9045)
                     /locus_tag="VIX62_12385"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY5861076.1"
                     /translation="VHSIIEFSHRALAFLVIVLTLATGLVLWRQPTTSRSVKWAAVLA
                     FPLVLSQAVLGGIVVATELNPWWVTAHFIAALVLIADVTFVAAACLTSDRSHPSGATN
                     DARFARLTLATLAAVGTLLLVGTYVRASNAQLVFTDWPLMDGRLIPPFGADGALAMFT
                     HRILALLGFSFVLWTMIRARSMARRSRPLVVLSTLAFALFCAQIASGAANVFSRLKPW
                     AVVAHVALSVLIWATLVALATVASLQARDTAPGGAPDTDLETAGDEAPAGEASWSDTV
                     NAYYRLMKPRIIVLLLITTVPAMILADHGIPSLWLIAATLVGGTLAAGSANSINMYLD
                     RDIDQIMRRTRERPLPAHQVSPERALAFGFWLGAFSFFFLASTVNVLAATLSLAAIAF
                     YVFVYTMWLKRSTVQNIVIGGAAGAAPALVGWAAVTGSLALPAWILFAIVFVWTPPHF
                     WALSMRFAGDYAAAGVPMMPVVRGERETRRQILLYSLLLFATTLVLYPVGDMGPIYLT
                     AAVGLGGMFVYRALVLWRRPDANASWRLFTYSLVYLAALFGAVALDALVPLRG"
BASE COUNT         1410 a         2993 c         3076 g         1566 t
ORIGIN      
        1 gatggtcggg tacttcgtct tcctggcctt caagcgcgag ccgatctggc aggtctcgcg
       61 ggcggtggcg gtcgtgggcg tcgcggccaa cctcgtctcg atcgtcgcgc gtggactggc
      121 ggccgactgg gtgccgtggg gcaacatgta cgagtactcg gtgatgctcg cgatgctcgt
      181 ggtcgccggc tacctgatca tcgtcgaagg catgtacaag gtccgcacgc tcggcgggtt
      241 cgcgttgatg ttcagcgtgc tgacgatggc ggtggcggtg tcgttcctct acgtcggacc
      301 cgggccactc gtgcccgcgt tgaactcgta ctggatcaag atccacgtcc aggccgcgat
      361 ctgggggtcg tcgttgttcg cgctcggcgg gatcgttacg atcctgtacc tggtccagga
      421 ctggcgtgag cgccggcagg tggaggcgct gcgcggatcg gggccgccgc cgatcatggg
      481 cggggcgctc gatctcgatc tgaacgcgcc cgacgacctg gaccgcgatg tcgacgaggt
      541 gccggtggcc gccgccgcac cgcgagcgag ccggttgccg tcggcccaga ccctcgaccg
      601 ggtggcgtat cggatcatcg ccttcgcgtt cccgatctgg accttcgcgg tgatcgccgg
      661 cgccatctgg gcccaggagg cctggggccg ctactgggga tgggacccga aagagacgtg
      721 gtcgttcatc acgtggacga tcttcgcggg atacctgcac gcgcgcgcga cgtccggttg
      781 gaagggtcgt cgcgccgccg tgatcgccct cgtcgggttc gtctcactgc tcgtcacgta
      841 ctacgtcgtg aacctgtgga tcgtggggct gcactcgtat gcgaagtagg ttcggcccgg
      901 tgcaaagtcg gcgagcaccc ggtggtacag tgcgcgcgtc tcggcccccg gcgggctccg
      961 aggccgaccc acgagggaga gggaacgtgc gcaagaaggt gctctggggc gtcgggctcg
     1021 cggccctggc cctgctgctg gcggcgtgcg ctcccgacgc cacgcagagc acgctcaagc
     1081 ccgtcggtga gcccgccatc gagcagaagc gcctcttcat cgccgtgatc tggatcgcgg
     1141 ccgccgtctt cgtgatcgtc gagggcggga tcctgttcct cgtgatccgc taccgccatc
     1201 gcaagggtcg cgagcgcatg ccgaagcaga cccacggcaa cacgcggctg gagatcggct
     1261 ggacgatcgc gcccgcgttg gtgctggcgg tggtgatggt ccccaccatc tcgatgatct
     1321 gggatctggc gcgtccgccg tcgcccgacg cgctcaacat caccgtgcag ggctaccagt
     1381 ggtggtgggg cttccaatac tcggactccg acatgaccgt ggggtcgggt gaacagcgac
     1441 cgatcgagat cgccgacgtc atggtggtgc cgaccggccg cgagatctac ctctcgctcg
     1501 aggccgaggg cggtggagcc aagaccaccg acggcacgcc cgacttccag gtgatccact
     1561 cgttctgggt ccccgaactc ttcggcaagc aggacgtcgt gccggggcac acgaaccaca
     1621 tcacgttcca ggtcgacgag gcgggcacgt acaccggcca gtgcgccgag ttctgcgggc
     1681 tccagcacgg ccggatgaag ctccgcgtcg tggcgctcga tcccgacgac tgggagctgt
     1741 gggtcgtcaa ccagcagcag gggccggcgc tcgtctccga tccgctcgcc aagcagggcg
     1801 aggagatctt catgaacccg ctgtcgggtg gccggggcgc ctgcaccgcg tgccatgcgg
     1861 tcggcgacgt gggtgcggcg gccgcgccga acctcagtca cttcaacgaa ccgacgcacg
     1921 agtgtttcgc gggctgcaac tgggaaacgt cagacgtgga cgcgctcaaa gcgtggctgc
     1981 gcgatcccga cgccgtgaag atgggggcca agatgcccaa ctaccagctc agcgatgacg
     2041 agatcgacgc gctggtggcc tacctgtaca gcctgacgtg atgggagcga acgcccgacc
     2101 atggcaagcg tgagcgttac cgggacctcg agccgagggt tgcgacggcc gtcgtcgttg
     2161 accggtttct ggagctggtt caccacggtc gaccacaaga agatcgggat catgtacggg
     2221 gtgtccgcgt tcgtattctt catcgtgggc gggttggaag ccctgctgat ccgcgcccag
     2281 ttggcccagc ccgacggtac gctcctcaac gccgatcagt acaaccagat cttcacgatg
     2341 cacgggatca cgatgatctt cctgttcgtg atgccgatgt cggcggcctt cttcaactac
     2401 ttcttgccct tgatgctcgg ggcgcgcgac gtcgcgttcc cacggctgaa cgcctacagc
     2461 ttctgggtgt tcctgctggg cggcctgttc ttgtactcga gtccgttcct ggggggagcg
     2521 ccgaacggtg ggtggttcgg gtatgccccg aactccacca cggatccatc gatcggcatg
     2581 tccttctatg cgctcggcct cctgatcacc ggtatcgctt cggcggcagg tgccgtcaac
     2641 ctggcgatca cgatcatcaa catgcgggca ccgggcatgt ccctgttccg catgccggtg
     2701 ttcgtgtgga tggggctggt cgtgcagttc ctgctggtct tcgcgctgcc gatcatcacg
     2761 gtgggcctgt tcgagctgtt gttcgaccgc cggtggggga cgcacttctt cgatccgacg
     2821 gccgggggcg atccgatcct gtggcagcac ctgttctggt tgttcggcca tcccgaggtg
     2881 tacatcttga tcctgcccgc gttcgggatc atcagcgaga tcatcccggt cttcgcgcgc
     2941 aagccgttgt tcggctacca gttcatggtg ttctccggca tcgcgatcgg gttcatcggc
     3001 ttcggcgtgt gggcccacca catgttcgcg gcggggttgg ggccggtcgc gaacacggcc
     3061 ttcggtgtct caacggccat catcgcgatc ccgaccggcg tgaagatctt caactggatg
     3121 gcgaccctgc acggcggcga tcttcgcttc accagcccga tgctgtatgc gatcggggcg
     3181 gtgacgatgt tcgtgatcgg cggactgtcg ggcgtgacgc acgccgtcgt gccgtcggac
     3241 taccagcaga cggacacgta ctacgtcgtt gcccacttcc attacgtgat cttcggcggc
     3301 agcgtgttcg ccttcttcgg ggccttccac tactggtggc cgaaggtgtt cgcgcgccag
     3361 ctctccgagg cgctgggcaa ggtggccttc tggatcctgt tgatcgggtt caacctgacc
     3421 ttcgggccca tgcacatcct cggactgtcg ggcatgatcc gccgcgagta cagctacccc
     3481 gagtcgctgg gcctgacgtt ctggaaccag atcgcgacct tgggggcgtt cctgatcgcg
     3541 ctgtccgtgc tgacgttcat ggtgaacgtc gtgcgaacgc acatccggaa gcagccgctg
     3601 gcctcggaag acccctggga cgcgcgcacc ctcgagtgga tgaccgcgtg cccgccgccg
     3661 gtgtacaact tcgaggagat cccccaggtc cactcgctcg atgagttctg gcaccgcaag
     3721 tacgtcgagg acgagaagac gggcacgctg gtcccggcgc aggccggcgc cgcccccgag
     3781 cacgaagagg atcacgcgga ggccgggcac gggatccatc tgcccagccc ctcgtactgg
     3841 ccgctggtct ccgcggtggg cttgcccgtg atcgcctacg gcgtcctcta ctcctggtgg
     3901 ctggtcgccg ccggggcgct cgtcgtcctg gttggcttca tgggctgggc cgtcgaaccc
     3961 tcggtggcgg aggactgacc atggcgatgg cgatcgagaa gccgcacgcc ggtgaaggcg
     4021 gagagcacga gaccaccacc gggctgccga acaccaagct cgcgatgtgg ttgttcctgg
     4081 cctccgagtg cctcctgttc ggcgccctga tcagcaccta cgtcctgtac cggggcgcca
     4141 gcaccgtcgg cccgtacccg gccgacgtgt tcgacatccc gtacacgtcg gtgtcctcgt
     4201 tcgtgctgct ggcctcgtcg ctgaccatgg tcctggcgct caccgccgcc cagaagcggg
     4261 acgtgccgcg catgcggctg tggctgctgg ccaccgccct gctgggactg accttcgtgg
     4321 gcggtcaggt gtacgaattc acagccttct accacgaggg cctcgcgctc tcgacgaacc
     4381 tgttcggcac caccttctac gtgctgaccg gcttccacgg ggtgcacgtc accgtgggga
     4441 tcttcatgct gctgtcgttg gtgggcctgt ccggcgccga tcgcctgccc gacgatgccg
     4501 ggttcccggt ggagatggtg gggctctact ggcacttcgt ggacgtcgtg tggatcatca
     4561 tcttcaccgt ggtctacctg atcccgaccg gcccgacgcc ggccaactag gagcacaccg
     4621 tggccgacac cgaaaccccg ttccagcaag agctcggaca ggaggatctg gctgaccccc
     4681 gcgagtcgca ggcggtgcac cgcgagcacc ccagcgccaa ggagtacatc cgcatcggga
     4741 tcatcctggc ggtgctcacc gctctggaga tcgccgcctc ctacgccgac gtgcggccgg
     4801 cgatcctgat cccgacgctc tacgccctgg cgttcgtgaa gttcgcattg gtcgtgttgt
     4861 ggttcatgca cctgaagttc gacgaccggc gctacgcacg gttcttcgtg atggggatcg
     4921 cgctcgcggg cacgctgtat ctgatcgtgt tgatcacgtt caaggtgttc ctgaggtagg
     4981 cggtgaccga catgcttccc gcgtggcacg tgcaccccga cgtctggctg ctgttcggtt
     5041 cgatcatcgc ggcctacctg atcgccggac gccggcacga gcgcgccacg ggcgaagcca
     5101 ccgaacccag gaagcgtcgg ttgttcctcc tcggcatggg cgtgttgtgg ctgggggccg
     5161 actggccgat ccacgacctg gccgagcgct acctgtacag catgcacatg acccagcacc
     5221 tgctgttcac gctggtggcc gcgccgatcc tcctcgccgg gatgcccgtg tggctgttgc
     5281 gggagctgct ggcgccggcg ccgatccgcc gcatcttccg gttcatgacg cgtccgctgg
     5341 tcgcgttgat cttcttcaac gcggtgctgc tgttcacgca ctggcccgac gtggtggcgg
     5401 cgtcggtggg atccgaactc gtccacttgg ggctccacgt gctgatcgtc acctccgccc
     5461 tggtgatgtg gtggccggtg atgtcgccgc tgccggagat gccctcgttg cccccgcccg
     5521 ggcagatgct ctacctgttc ctgcagtcgc tggctcccac gatccccgcg tcgttcctga
     5581 cgttcggaca caagcccttg tatccggtgt acgccacgtt cccgcggatc tgggggatcg
     5641 acgcgctcac cgatcagctg atcgcgggat tgatcatgaa gctcctgggc gggctgctgc
     5701 tgtggggggt catcgccgtg atcttcttcc gctggtacaa ccaggagcag cgtgacgggc
     5761 ttgacccgac gacccatcag aacatcagac gagagatcgg tgcggagttg agtcgatgag
     5821 cggacgcgag aaccgacaga gcctgctgct gccgatcctg ttgccgttgg ggatcctcgg
     5881 ggtgatcgcc ttggtgttgt acgggttctc ccgcatcctg ctcaacatca cgtccacggc
     5941 ggccacggtc ctggcgtgca tcgccgccgt cgcggtggtc gccctggcca cgttcgtcgc
     6001 ctctcgtccc cgggtggggg cgtcctcgct gttctcggtg gtgggcggtg tcgccggcgt
     6061 agcgatgttg gcgggcggtc tcgcgctcct cgtagcaccc ccggccgaag agggggagga
     6121 cggcggcacc gagggtccgg tcgtgacgct ggccgccccg gtcggcgcgg cggccgacgg
     6181 cttcagcacg aacaagctct ccgtgcccgc cgaggaggcg ttcacgatcg acttcgacaa
     6241 cgaagagacc ggcgtccagc acaacgtcgt gatcttcgac ggcaaggacg ccgaggcgcc
     6301 ggcgctcttc accggtgagt tgatcatcgg tccgaagacg acgccctact ccgtcgacgc
     6361 attgcccgag ggcaattact tcttccactg cgagatccac ccgacgacga tgacgggcga
     6421 tgtcatcgcc aagcccggca ccggcggcga acccagcggt gacgagggcg gcggcgccgg
     6481 ctcgcccacg atcgcggcgc aggggacggc gttcaacacc tctgagctcg cgttcaaggc
     6541 cgagacggcc acgccgctcg tgttcgacaa ccaggacgat gccgccgtca ccggcccgca
     6601 caacgtgtcg atctacgacg gcgatgccgc gctgttcacc ggcgacctca tcaacggccc
     6661 gcaacaggtg acgtacgaca tccctgccct gaaaccgggc gagtaccagt tccgctgcga
     6721 cgtccacccg gagatggccg gggccgtcac ggtcacctga cgacatcggt tcgccactga
     6781 cgtcgtgtcc gcccggcggt agcgtggccc cgtgcgcaag gtggcggtga tcgtagtggc
     6841 ggcgttggtg gtgagcctgt tggccgcctt cgtcttccgt ccccgcggcg gcgatccgcg
     6901 catcgagggc ggcgtcgtcg agatggatca gcccatcccg gatctgacgg cgatcgacac
     6961 ggtctcgggc gacccgctgc cgccggacgc gttcgagggc tcggccaccg tgatcaacgt
     7021 atgggccaac tggtgcgcac cgtgccggca ggaacagcct gccctcgtgg ccacccaggc
     7081 gcgatacgcg gaccaggacg tgcgcttcgt ggggatcaac tacaacgacg atctcgaact
     7141 ggcgcgccgg tgggtggacg atttcgaggt cacctacccc agcctctacg acccgcaggg
     7201 gcggaccgcc gcactgctgg acttcccgtt cctgcccgac acctacgtcg tcgacggcgc
     7261 cggcacgatc cgctacgcga tctacggaga gaccgacgag gacgaactgt cgggcctgat
     7321 cgacgacgtg ctggctggcg acaccgcctc gagcggcacc tagccgcgga gcggcaccag
     7381 cgcgtcgagc gcgacggcgc cgaacagcgc cgcgaggtag acgagcgagt acgtgaacag
     7441 ccgccaggac gcgttcgcat cgggacgccg ccacagcacc agcgcacggt agacgaacat
     7501 cccaccgagc ccgaccgcgg ccgtgaggta gatcggcccc atgtcgccga ccggatagag
     7561 caccagggtc gtcgcgaaca gcagcaggga gtacaggagg atctgtcgcc gggtctcccg
     7621 ctcgccgcgg accactggca tcatcggcac gcccgccgcc gcgtagtcac ccgcgaaccg
     7681 catcgacaac gcccagaagt gcggcggcgt ccagacgaac acgatcgcga acaagatcca
     7741 cgccggcagc gccagggagc cagtgacggc ggcccacccg acgagggcgg gtgccgcccc
     7801 ggcagcgcca ccgatcacga tgttctgcac cgtggagcgc ttgagccaca tcgtgtagac
     7861 gaagacgtag aaggcgatcg cggcgaggga cagggtggcc gcgagcacgt tcacggtcga
     7921 ggcgaggaag aagaacgaga acgcccccag ccagaacccg aacgccagcg cccgttcggg
     7981 gctcacctgg tgggccggga gcggccgttc gcgggttcgc cgcatgatct ggtcgatgtc
     8041 gcggtccagg tacatgttga tgctgttggc cgatcctgcc gccagggtgc cgccgaccag
     8101 ggtggcggcg atcagccaga gcgaggggat gccgtggtcg gcgaggatca tggcgggcac
     8161 cgtggtgatc agcagcagga cgatgatgcg gggcttcatc aaccggtagt aggcgttgac
     8221 ggtgtccgac cacgaggctt cccccgccgg cgcctcatcg cccgcggtct cgagatcggt
     8281 gtcgggggcg ccacccgggg cggtatcccg ggcctgcaac gaggcgacgg tggccagggc
     8341 gaccaacgtg gcccagatca agacggacag ggccacgtgc gcgacgaccg cccacggctt
     8401 caaccgtgag aacacgttgg ccgcgcccga tgcgatctgg gcgcagaaga gcgcgaacgc
     8461 cagggtggac agcaccacca ggggacggga ccgcctcgcc atggatcgcg cgcggatcat
     8521 cgtccacaac acgaacgaga acccgagcaa cgccaggatc cggtgggtga acatcgcgag
     8581 ggcaccgtcg gcgccgaagg gcgggatcag cctgccgtcc atcaacggcc agtcggtgaa
     8641 gaccagttgc gcgttgctgg ctcggacgta ggtgcccacc agtaggagcg tgccgaccgc
     8701 ggccaacgtg gcgagcgtga gcctcgcgaa ccgcgcatcg ttggtcgccc ccgacgggtg
     8761 agaccggtcg gacgtcaggc atgccgccgc cacgaacgtg acatcggcga tcagcaccag
     8821 ggccgcgatg aagtgtgccg tgacccacca ggggttgagc tcggtcgcga ccacgatccc
     8881 gccgagcacc gcctgggaca gcaccagggg gaacgcgagc acggccgccc atttcacgga
     8941 ccgggacgtg gtcggttgcc gccacagcac caggccggtg gcgagcgtga gcacgatcac
     9001 gaggaaggcc agggcgcgat gcgagaactc gatgatggaa tgcac
//
LOCUS       DATDBZ010000535         3069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1194820, whole genome shotgun sequence.
ACCESSION   DATDBZ010000535 DATDBZ010000000
VERSION     DATDBZ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194820"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VIX62_12390"
     CDS             <1..925
                     /locus_tag="VIX62_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5861077.1"
                     /translation="PMAAGALPVFVDVTPQTWTLDPEQIEAAITDRTKAIIPVHLGHQ
                     MADMDRIMEIARKHGLAVIEDCAHAHGQRWRDQGAGCIGDFGSFSHQSSKILTSGAGG
                     SLLTSDDTLARRAHSIIDCGRAKDADEKEFTFGANYRLGELNAALLVVAMKKFPPQQE
                     ARAAAGEAFEQLAAQIPGVRVMPRDERITRWSFYNYILAIDPEAFGGATNDVVCEAME
                     AEGIPAEVQYPSMNRYELFQPSLSRMPVAVAMADRLDMQQMSFPVAEAAGQRESVYFM
                     ENVFRDGSRGIEDAAEALAKIQRNASELPKN"
     gene            complement(941..2227)
                     /locus_tag="VIX62_12395"
     CDS             complement(941..2227)
                     /locus_tag="VIX62_12395"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEY5861078.1"
                     /translation="MKVAVVGGGSTYTPELVEGFVTRGDRLPVDELVLLDIDRERLDV
                     VGSLAERIMRRVGWNGTLTLTDDRVEAIDGADFVIVQLRVGGNQARYRDETIPPAFGC
                     IGQETTGPGGFAKALRTVPVVLELAELTAERGAAGAWFVDFTNPTGLVTQALLDQGHR
                     AIGLCNVAIGFQREFAASYGVEPDHVRLDHVGLNHLTWERAVYVDGEDKLPEMLASLD
                     DPTSAYRAILEEDDADPELVKALGAIPSYYLHYYYDADRVLAAQQAGQTPRAEEVMKI
                     EAGLLELYRDPALDTKPKLLEERGGAFYSDAAAALIASLHAGTGDALVVNLRNDGAIP
                     NLPDDAVVEITAMIDRDGAHPLRTAPLAPEMHGLVAHAKAYEMLAIEAAVSGSRTTAL
                     KALMTNPLVGDHRRAEPLLDALLAANRQLLPRFFPN"
     gene            2260..>3069
                     /locus_tag="VIX62_12400"
     CDS             2260..>3069
                     /locus_tag="VIX62_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY5861079.1"
                     /translation="MKSLGNFPDPFMAEIAGQPDALRRAAARGVEQADRVARLTELAP
                     GRTLIFTGMGSSYDACYPAVAELAHAGVPAIMVDSAELLHFRGGLLGPATLLIAVSQS
                     GESAEVVRVAGAARGRSDPPAIVAVTNGGTNPLAALADVVFDTAAGPETGPSTMTFAG
                     SLVAVGTIGRVLASHRRDAALEHLEAETELAAVAIERILEDAGLPDSLATWLEGRTTV
                     VILGRGPARAAAEMGALTIKEAVAMPIESLQTAQFRHGPLEMVGPSLAAIVI"
BASE COUNT          503 a         1048 c         1021 g          497 t
ORIGIN      
        1 ccccatggcg gccggtgcat tgccggtatt cgtcgacgtg acaccgcaga cctggacgct
       61 cgaccccgag cagatcgaag ccgcgatcac cgatcgcacc aaggcgatca tcccggtgca
      121 tctgggccat cagatggccg acatggatcg gatcatggag atcgcgagga agcacgggct
      181 cgccgtcatc gaagactgcg cgcatgcaca cgggcagcgc tggcgcgacc agggagccgg
      241 ttgcatcggg gacttcggct cgttcagcca ccaatcttcg aagatcctga cgtcgggcgc
      301 gggtggctcc ctgctcacca gcgacgacac cctggcgcga cgcgcgcact cgatcatcga
      361 ctgcggacga gcaaaggatg ccgacgagaa ggaattcacg ttcggcgcca actatcggct
      421 cggtgaactg aacgccgcgc tgctggtcgt cgcgatgaag aagttccctc cccagcagga
      481 agctcgagcc gccgccggcg aagcgttcga gcagctggcg gcgcagatcc ccggggtccg
      541 cgtgatgccg agggatgagc gcatcactcg gtggagcttc tacaactaca tcctcgcgat
      601 cgatcccgag gcgttcggcg gggcgaccaa cgacgtggtg tgcgaagcga tggaggccga
      661 gggcatcccc gccgaggtgc agtacccgtc catgaaccgc tacgaactct tccaaccgtc
      721 gctgtcacgc atgccggtcg cggtggccat ggcggatcgg ctcgacatgc aacagatgtc
      781 gttccccgtc gccgaggccg cggggcagcg tgagtcggtc tacttcatgg agaacgtctt
      841 ccgtgacggc agtcgtggca tcgaggatgc ggcggaagcg ctcgcgaaga tccaacgcaa
      901 cgcctcggaa ctgccgaaga actgaccggc gagggccggg tcagttcggg aagaagcgcg
      961 ggagcagctg ccggttcgcc gcgagcagcg cgtccaatag cggttccgct cgtcgatggt
     1021 cgcccaccag ggggttggtc atgagggcct tcaacgccgt cgttcggctg ccgctcacgg
     1081 cggcctcgat cgccagcatc tcgtacgcct tggcgtgcgc taccaacccg tgcatctccg
     1141 gtgccagcgg cgcggtgcgg agcgggtgcg caccatctcg gtcgatcatg gccgtgatct
     1201 ccaccaccgc gtcgtccggc aggttcggga tggcgccgtc gttgcgaagg ttcaccacca
     1261 gggcatcacc ggtgccggcg tgcagcgatg cgatgagcgc ggccgcagca tcgctgtaga
     1321 acgcgcctcc tcgctcctcc agcagcttcg gcttggtgtc gagcgcgggg tcgcgataga
     1381 gctcgagcag gccggcctcg atcttcatga cctcctcggc gcggggcgtc tgcccagcct
     1441 gctgagcggc gagtacgcga tcggcgtcgt agtagtagtg caggtagtag ctggggatcg
     1501 cgccgagcgc cttcaccagt tctggatcgg cgtcgtcttc ctccaggatc gcgcggtagg
     1561 ccgatgtcgg atcgtcgagg gatgccagca tctccggcag cttgtcctca ccgtccacat
     1621 acaccgctcg ctcccacgtc aggtggttga ggcccacatg atccaggcga acgtggtctg
     1681 gctccacccc gtaggacgcg gcgaactctc gttggaaccc gatcgccacg ttgcacagac
     1741 cgatcgctcg atggccctgg tccaacaacg cctgggtcac caggccggtg gggttcgtga
     1801 agtcgacgaa ccaggctcct gccgctcctc gttctgccgt cagctccgcc agctccagga
     1861 ccacgggcac ggtgcgcaac gccttcgcga agccgcccgg gcctgtggtc tcctgaccga
     1921 tgcagccgaa cgcgggcggg atcgtctcat cgcggtatcg cgcctggttg ccgccgaccc
     1981 gcagctgcac gatcacgaag tccgcgccgt cgatcgcttc gaccctgtcg tcggtgagcg
     2041 tcaacgtgcc gttccacccc acgcgccgca tgatgcgttc cgcgagggaa cccaccacgt
     2101 cgaggcgctc cctatcgatg tcgagcaaca ccaattcgtc gacggggaga cggtcgccac
     2161 gggtcacgaa cccctcgacc agctcgggcg tatatgtgct cccgcccccg acgaccgcca
     2221 ccttcacacc catggtccgg gcatcgtaca ctcgcgccga tgaaatcgtt aggaaacttt
     2281 cctgacccat tcatggccga gatcgcgggc caacccgatg ccctccgccg agctgccgcg
     2341 cgaggcgtcg agcaggccga ccgggtcgcc cggctcaccg aactcgcacc ggggcgcacg
     2401 ctgatcttca cgggtatggg gagttcctac gatgcctgct atccggcggt ggccgaactc
     2461 gcgcacgccg gagtgccggc gatcatggtg gactccgccg agctgctgca cttccgcggt
     2521 ggactgttgg ggccggcgac actgttgatc gccgtgagcc agtcgggcga gagcgcggaa
     2581 gtggtgaggg tggccggagc ggcgcgcgga cgatcggacc cgcccgcgat cgtcgccgtc
     2641 accaacggcg gaaccaaccc gctcgctgcg ctggccgacg tggtcttcga cacggccgcg
     2701 ggaccggaaa ccggcccgtc gaccatgacg ttcgcggggt cgctggtcgc ggtgggcacg
     2761 atcgggcgcg tcctcgcgag tcatcgtcgc gacgctgcgc tcgaacacct ggaagccgag
     2821 accgagctcg ctgcggtcgc gatcgaacgc atcctggagg acgccggtct gcccgactcc
     2881 ctcgcgacgt ggctcgaagg gcgaaccacc gtcgtgatct tgggccgtgg ccccgcgcgc
     2941 gccgccgccg agatgggcgc gctcacgatc aaggaagccg tcgccatgcc gatcgagtcg
     3001 ttgcagaccg cgcagttccg gcacggcccc ttagagatgg tcggcccgtc gttggccgcc
     3061 atcgtgatc
//
LOCUS       DATDBZ010000536         2638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_165143, whole genome shotgun sequence.
ACCESSION   DATDBZ010000536 DATDBZ010000000
VERSION     DATDBZ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165143"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VIX62_12405"
     CDS             complement(<1..378)
                     /locus_tag="VIX62_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861080.1"
                     /translation="MDDLRLPKSRSAPAEPPMAPAPPIPRRARTIDEREFRAWLWVTT
                     SLLALALVAVSVPLAATLYGVHLLAAFATSLALAGALPLAVRWPWAAAGLATAGVLVF
                     ALLSAGSEGAPWPWPVTTIISQGA"
     gene            550..2145
                     /locus_tag="VIX62_12410"
     CDS             550..2145
                     /locus_tag="VIX62_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEY5861081.1"
                     /translation="MSDAQVDAAAPRPREQWTGQVGFILSAIGSAVGLGNIWRFPGVA
                     YENGGGAFLIPYLIALITAGIPILFLDYAIGHRFRGSAPMAFRRLGGKWLEHLGWFQV
                     TICFVIGLYYTAVVAWALSYFVFSFDLRWGDDPATFLTGDFLQVSDPGISFEFVPGVL
                     IPLAIVWIAVIFVLAAGVAKGLQRANVIFLPLLVVAFLVLVVRALFLDGAAEGLNALF
                     TPNWEALGDPDVWIAAYSQIFFSLSIAFGIMITYSSYRARRANLTTPGLVVAFANSSF
                     EILAGIGVFATLGFFAFQQGVAIDELEGLTGVGLSFITFPAIVSQMPGGPIFGVLFFG
                     SLTMAGFTSLISVMQVVSAGFQEKFGLTVRQAAIRVGGVAAILSILLFSTTTGLLALD
                     VADQWANNIGIVASAILTSVLVLWVLRRGPELRTHLNAVSTFKVGRIWLVLTSVLAPI
                     VLGYMLVSRIVTLTVEGYGGLPGWYLVAIGWGTIIAMLVGTFVLNALRWRQDPDRFAP
                     WPTYPPGGAAATAGRNASDGGAS"
     gene            2142..2309
                     /locus_tag="VIX62_12415"
     CDS             2142..2309
                     /locus_tag="VIX62_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEY5861082.1"
                     /translation="MTPIAIFFLVLAIVLVWGGLIASVLFLRRRPELDQYPAGGHDDH
                     REDDAPIIRDT"
     gene            complement(2351..>2638)
                     /locus_tag="VIX62_12420"
     CDS             complement(2351..>2638)
                     /locus_tag="VIX62_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5861083.1"
                     /translation="RTYGVLGGLIALLLWIWLSNLAMLFGAVLDTEVERARQLHAGVD
                     AAEQVRLPLRDDRQIIANRTRRVADVRASERMRGDAVLDGITPPAGRPPHG"
BASE COUNT          352 a          927 c          896 g          463 t
ORIGIN      
        1 cgccccctgg ctgatgatcg tcgtgacggg ccacggccac ggcgcgccct cggacccggc
       61 tgagagcagc gcgaacacga ggacaccggc ggtcgcgagc ccggccgcgg cccatggcca
      121 gcggaccgcg aggggcagcg ctccggcgag tgcgaggctc gtggcgaagg ccgcgagcag
      181 gtgcacgccg tacagggtcg ctgcgagcgg caccgacacc gccacgagcg cgagcgcgag
      241 cagcgacgtc gtcacccaca accaggctcg gaactcgcgt tcgtcgatcg tgcgcgcacg
      301 acgggggatc ggtggggccg gcgccatcgg cggttctgcc ggtgcggatc ggctcttcgg
      361 gagtcggagg tcgtccatga tgctgtcact ctggcacgcg cgcggtctcg cgacatccct
      421 ccggcgagcc attcccgcct cgtaccgtcg tagggggcct cggggctcgc ggtgccggtg
      481 cgaccgctgc tacggtgccc atgacggacg gggttcgtgc gtggcgcgga ccgcttgaaa
      541 ggggtcaccg tgtcagacgc acaggtcgac gccgccgcac cgcggccgcg agagcagtgg
      601 acgggacagg tcgggttcat cctgtcggcc atcgggtcgg cggtcggact cggcaacatc
      661 tggcggttcc cgggcgtcgc atacgagaac ggcggcggcg cgttcctcat cccctatctc
      721 atcgcgctca tcaccgccgg aattccgatc ctgttcctcg actacgcgat cggccaccgg
      781 ttccgcgggt ctgcgccgat ggcgttccgc cggctcggag gcaagtggct cgagcacctc
      841 ggctggttcc aggtgacgat ctgcttcgtc atcggcctct actacacggc cgtcgtcgcg
      901 tgggcgctca gctacttcgt attctcgttc gacctgcggt ggggtgacga ccccgcgacg
      961 ttcctcacgg gagacttcct gcaggtcagc gacccgggca tcagcttcga gttcgtgccc
     1021 ggcgtgctga tcccgctcgc gatcgtctgg atcgcggtga tcttcgtgct cgcggccggc
     1081 gtcgcgaagg ggctgcagcg agccaacgtg atcttcctgc cgctgctcgt cgtcgcgttc
     1141 ctcgtgctcg tcgtgcgtgc gctgttcctc gacggcgccg ccgaaggact caacgccctg
     1201 ttcacaccga actgggaggc cctcggcgac cccgacgtct ggatcgccgc gtacagccag
     1261 atcttcttct cgctctcgat cgcattcggc atcatgatca cctattcgtc gtaccgcgcc
     1321 cgtagggcga acctgaccac gcccggcctc gtggtggcgt tcgcgaactc gtcgttcgag
     1381 atcctcgcgg gcatcggcgt gttcgccacg ctcgggttct tcgcgttcca gcagggggtc
     1441 gccatcgacg aactcgaggg gctcaccggc gtggggctct cgttcatcac gttccccgcg
     1501 atcgtgtcgc agatgccggg tgggccgatc ttcggggtgc tcttcttcgg ttcgctgacg
     1561 atggccgggt tcacgtcgct catctcggtg atgcaggtgg tctccgccgg cttccaggag
     1621 aagttcggcc tcaccgtgcg gcaggcggcc atccgcgtcg gcggcgtcgc cgcgatcctc
     1681 tcgatcctgc tgttctccac gaccaccggg ctgctcgccc tcgacgtggc cgaccagtgg
     1741 gcgaacaaca tcggcatcgt ggcatcggcc atactcacct cggtgctcgt gctctgggtg
     1801 ctgaggcggg gacccgagct gcgcacgcac ctgaacgccg tgtcgacgtt caaggtcggg
     1861 cgcatctggc tggtgctcac cagcgtgctc gcgccgatcg tgctcggcta catgctcgtc
     1921 tcacgcatcg tcacgctgac ggtcgagggc tacggaggac taccgggctg gtacctcgtc
     1981 gcgatcgggt ggggaacgat catcgccatg ctcgtcggca cgttcgtgct gaacgcactg
     2041 cggtggcgcc aggaccccga tcggttcgcg ccatggccga cgtacccgcc gggtggcgcc
     2101 gcagccaccg ccggtcgcaa cgcatctgac ggaggtgcat catgactccc atcgcgatct
     2161 tcttcctcgt gctcgccatc gtgctcgtct ggggcgggct catcgcgagt gtgctcttcc
     2221 tgcgcaggcg ccccgagctc gaccagtacc cggcgggcgg ccacgacgac catcgcgaag
     2281 acgacgcgcc gatcatccgc gacacctgat tcgagcggat gtcccggcgc gcgtcatgcg
     2341 cacccgaggg tcacccgtgc ggtggccgtc ccgccggggg cgtgatgcca tcgagcacgg
     2401 cgtcgccccg catgcgctcc gacgcgcgca catcggcgac cctccgggtg cggttggcga
     2461 tgatctgccg gtcgtcgcgc agcggaagtc gcacctgctc ggcggcatcg accccggcgt
     2521 gcagttgccg tgcccgctcg acctcggtgt cgagcaccgc gccgaagagc atcgccaggt
     2581 tcgacagcca gatccagagc agcagggcga tcaggccgcc cagcacgccg tacgtgcg
//
LOCUS       DATDBZ010000537         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_3042052, whole genome shotgun sequence.
ACCESSION   DATDBZ010000537 DATDBZ010000000
VERSION     DATDBZ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3042052"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1128)
                     /locus_tag="VIX62_12425"
     CDS             complement(268..1128)
                     /locus_tag="VIX62_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861084.1"
                     /translation="MDNSGGFTTPPPPPPPPGGEGGAGGPGLPPRGIGEILSTAFEIY
                     KANAQNLLMIVAIVVVPLTFISAFIGGVVFAPDTETVLGTTVEVSTRTATAGLIVLLV
                     GVVIGVIINAVLQAAIMRGAAQGSIGDPVDIDASYKWGFARFGSVLLISFLVGLAVLG
                     GFILLIIPGIIFMVMFAVAIPALVVENLRGTDAMSRSWNLVKGNFWHALGLIIVAGII
                     TAVVGGIVGAIGGAISDNWFVAWIFQAIAQIVTAPFAAIVSVLLYLDLRARREALTAD
                     GLRAELNRSV"
     gene            complement(1210..1728)
                     /locus_tag="VIX62_12430"
     CDS             complement(1210..1728)
                     /locus_tag="VIX62_12430"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEY5861085.1"
                     /translation="MDDAPRRQSSFYFAPGSSWLNDPRDRGEVRFLDDPWELEPKVRE
                     RPVLSFGFPDTPYAVLLTWSGDWTMFEGYYVNIQSPLRLHGDTVDYTDWFLDVRIPPE
                     RDTYEWKDEHELAEAVQRGLLTRSEADDVRWAGERAIEQVLLHEPPFDHEWSAWRPDP
                     SWGPLDLPDTAG"
     gene            complement(1836..>2495)
                     /locus_tag="VIX62_12435"
     CDS             complement(1836..>2495)
                     /locus_tag="VIX62_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018847384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5861086.1"
                     /translation="DVVAFLVEPIQGEAGVVVPPEGYLRRARELCDEHRVLLIADEVQ
                     SGLGRTGRTFACEHEGVQPDVYVLGKALGGGIMPLSAVVSNRDVLGVFRPGEHGSTFG
                     GNPLACALGIEVLRLLGTGEYQRRSDELGTALIARLRAEAPAAAVSEVRGKGLWVGIE
                     LTPVAAPAREVCERLMEMGVLAKDTHETTVRLAPPLCVTQEDLDWAAERIVTALAERL
                     D"
BASE COUNT          402 a          872 c          867 g          354 t
ORIGIN      
        1 agtgctcgct caccgcctcg atgacgctgt cgggcacctg ggacccgccc ggggcgtcgg
       61 cgtagatcac gggccgccga tcgtgcgtgc gctcgagcgc ggggaagcgc gatcgcagca
      121 cggagagatc gggcgcggtg ctcacgggca taggttcgcc ggtcggggcc ggtgcgacaa
      181 ccctggcgag ggtggcgcgg gtcgtgcccg caacgcgacg gaggcgcccc gaggggcgcc
      241 tccgcaggcc gtcgtgtgag gatcgtgcta cacggagcgg ttcaactctg ctcgcaggcc
      301 gtcggccgtg agggcttcgc gacgcgcacg cagatcgagg tacaacaaca cgctcacgat
      361 cgcggcgaac ggcgccgtca cgatctgggc gatcgcctgg aagatccacg cgacgaacca
      421 gttgtcgctg atcgcaccgc cgatcgctcc gacgatgccg ccgaccacgg cggtgatgat
      481 gcccgccacg atgatcagac ccagggcgtg ccagaagttc cctttgacca ggttccatga
      541 acggctcatc gcatcggtgc cacggaggtt ctcgaccacg agggccggga tcgccacggc
      601 gaacatcacc atgaagatga tgcccgggat gatgagcagg atgaacccac cgagcacggc
      661 gaggccgacg aggaacgaga tcagcagcac gctgccgaag cgagcgaatc cccacttgta
      721 cgacgcgtcg atgtcgaccg ggtcgccgat gctgccctgt gcggcccccc gcatgatggc
      781 cgcctggagc accgcgttga tgatcacgcc gatcacgacg ccgacgagga gcacgatgag
      841 tcccgcggtc gccgttcggg tgctcacctc gaccgtcgta ccgagcacgg tctcggtgtc
      901 cggcgcgaag accacgccgc cgatgaaggc actgatgaac gtcagcggca cgacgacgat
      961 cgcgacgatc atcaacaggt tctgcgcgtt cgccttgtag atctcgaacg cggtgctcag
     1021 gatctccccg atccctcgcg gtgggaggcc gggtcctcct gcgcctcctt cgccgcctgg
     1081 tggcggcggg ggcgggggcg gggtggtgaa gccacccgag ttgtccatgt ggatccccct
     1141 tctctgacgg tacggacggc ggcatcctac gtccgcccca gcatgtggtg tgggatgccc
     1201 tccctcacct cagcccgcgg tgtcgggcag atcgagcggt ccccacgacg gatcggggcg
     1261 ccaggccgac cactcgtggt cgaacggcgg ctcatgcagc agcacctgct cgatggcgcg
     1321 ttccccggcc catcgcacgt cgtcggcctc cgatcgcgtg agcaatccac gctgcaccgc
     1381 ctcggcgagc tcgtgctcgt ccttccattc gtaggtgtcg cgctcgggcg ggatgcgcac
     1441 gtcgaggaac cagtccgtgt agtcgaccgt gtcgccatgc aggcgaagcg gcgactggat
     1501 gttcacgtag tagccctcga acatcgtcca gtctccggac caggtgagca gcacggcgta
     1561 cggcgtgtcc gggaagccga aggagagcac gggccgctcg cgcaccttgg gctcgagctc
     1621 ccatgggtcg tcgaggaatc gcacctcccc ccgatcgcgt ggatcgttca gccacgaaga
     1681 accgggggcg aagtagaagg agctctggcg acggggcgcg tcatccacga ccctcaccgg
     1741 gcgcagggac aacgtgcgtg cctgccaggt ctcgcgcagg aagaccggtg tccccggggg
     1801 gagcggctcc cgctcagagc ccttcccgag cggagtcagt cgagccgctc ggccagagcc
     1861 gtgacgatgc gttccgccgc ccaatccagg tcctcctgcg tcacgcagag cgggggagcg
     1921 agccgcacgg tggtctcgtg ggtgtccttg gcgagcacgc ccatctccat cagccgctcg
     1981 cacacctcgc gggccggggc ggccacgggg gtgagctcga tgccgaccca caggcccttg
     2041 ccgcgcacct ccgacaccgc ggccgcgggc gcctcggcgc gcagccgggc gatgagcgcc
     2101 gtgccgagct cgtcggaccg gcgctggtac tcgccggtac ccagcaggcg gagcacctcg
     2161 atgccgagcg cgcacgccag ggggttgccg ccgaacgtgg aaccgtgctc accggggcgg
     2221 aacacgccga gcacgtcgcg gttcgacacc acggccgaca aaggcatgat gccgccgccg
     2281 agcgccttgc cgagcacgta gacgtcgggc tgcaccccct cgtgctcgca ggcgaaggtg
     2341 cgccccgtgc gcccgagccc cgactgcacc tcgtcggcga tgagcagcac gcggtgctcg
     2401 tcgcagagct cgcgggcccg gcgcaggtag ccctcgggcg gcacgacgac cccggcttcc
     2461 ccttggatcg gctcgacgag gaacgccacg acgtc
//
LOCUS       DATDBZ010000538         2108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_3042177, whole genome shotgun sequence.
ACCESSION   DATDBZ010000538 DATDBZ010000000
VERSION     DATDBZ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3042177"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..619)
                     /locus_tag="VIX62_12440"
     CDS             complement(83..619)
                     /locus_tag="VIX62_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4916 domain-containing protein"
                     /protein_id="HEY5861087.1"
                     /translation="MSESTDTRTTWLSKDDIETYRARMPVVYVDAVPVRVDANGTVTR
                     VGTLLGMQPDGSISRAVISGRILYGERVRDALVRHLEKDLGPVALPHIPPSPAPFTVV
                     EYFPDPDITGFHDPRQHAVSLAFVVPVAGDCRPSQDALDLAWLTPEEIVREDIRREMS
                     SGHDRLVRMALAHVGLLT"
     gene            complement(741..>2108)
                     /locus_tag="VIX62_12445"
     CDS             complement(741..>2108)
                     /locus_tag="VIX62_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5861088.1"
                     /translation="RAQRALLLTAQHDALTGLPNRALLGEQITEALHDAWREDVRPTV
                     LFIDVDRFKNINDSLGHAVGDQVLRLLGRRLRAAVPPHVRIGRISGDEFMALDATVRT
                     MGEALAFADLVLSVFHEPIHVGSGDMYITASIGVAVAKTNGSVSAEELHRNADIAMYR
                     AKDAGRNCVALFDDSMHERVAQRLQIESALHTALDRRELTMYYQPIVDLVTGEVLGFE
                     ALMRWHLADGRLVSPAEFIPIAEETGLIVPIGAWALLEGLMQLRAWIDDGVCRPECSM
                     SVNLSPRQLRDPNLLSSVGEALSRSNIAASQLWLEVTEGVMISEPEEALTTMRLIRAL
                     GVRLAIDDFGTGYSSLSMMRRFPLQRMKIDRAFVSGSADDDSARSLVHTIVAMADALG
                     LDVVAEGVESMAQLRVLRSLGCRQAQGFLMSPPVPPAVMRATIHQIEQTTAWSGGMHL
                     SRPQA"
BASE COUNT          331 a          709 c          742 g          326 t
ORIGIN      
        1 ggttcatgtc agcgatcccg cgcaaaggcg cgcctctgct gacacggatg gggtttgctg
       61 acaagaacgc gtgcggtcgc ggtcaggtga ggaggccgac gtgggccagt gccatgcgca
      121 cgaggcggtc gtggccgctg ctcatctccc ggcggatgtc ctcgcggacg atctcctcgg
      181 gcgtgagcca tgcaaggtcg agcgcgtcct gcgacgggcg gcagtcgccc gccaccggca
      241 cgacgaacgc caggctgact gcgtgttggc ggggatcgtg gaaccctgtg atgtcgggat
      301 cagggaagta ttcgacgacg gtgaacggcg ccggtgacgg cgggatgtgg ggcagcgcta
      361 ccggcccgag gtccttctcg aggtggcgca cgagcgcatc gcgcacccgc tcgccgtaga
      421 ggatccgacc ggagatcacg gcccggctga tcgatccgtc gggctgcatg ccgagcagcg
      481 tgccgacgcg cgtcacggtc ccgttcgcat ccactcgtac cggtacggcg tcgacgtaca
      541 ccacgggcat gcgggcccga taggtctcga tgtcgtcctt cgacagccag gtggtgcggg
      601 tatcggtgct ctcggacacg gttctccttc gacgggcgcc gaccacgggt agggacgatc
      661 atcgcacccc ggccgcccgg cgccagcacg ccgaccgccc ggcgccagcg cgccggctgc
      721 cggtaagccc tcctccggcc tcaggcttga gggcggctga ggtgcatccc cccggaccac
      781 gccgtcgtct gttcgatctg gtggatcgtc gcccgcatca cggccggcgg aaccggtggg
      841 ctcatcagga agccctgcgc ctgccggcaa ccgagcgagc ggagcacgcg cagttgcgcc
      901 atgctctcga cgccctcagc gacgacatcg agcccgagcg cgtcggccat ggcaacgatc
      961 gtgtgcacga gcgagcgggc gctgtcgtcg tcggcggaac cggagacgaa ggcgcggtcg
     1021 atcttcatcc gctgcagggg gaagcgacgc atcatgctca gcgagctgta ccccgtgccg
     1081 aaatcgtcga tcgccaggcg tacgcccagt gcacggatga gtcgcatggt cgtgagcgcc
     1141 tcctccggct cgctgatcat cacgccctcg gtcacctcga gccacagctg cgaggcggca
     1201 atgttggatc gagagagcgc ctcgccgacg gaggagagca ggttcgggtc gcgcagttga
     1261 cggggtgaga ggttcaccga catcgagcac tccggacggc acactccgtc gtcgatccac
     1321 gctctgagct gcatcagccc ctcgagcagt gcccacgcgc cgatcggcac gatcaacccg
     1381 gtctcctcgg cgatcgggat gaactcggcc ggcgacacca ggcggccatc cgcaaggtgc
     1441 cagcgcatca gcgcttcgaa gccgagcacc tcgccggtca cgagatcgac gatgggttgg
     1501 tagtacatcg tgagctcgcg ccggtcgagc gccgtgtgca gcgccgactc gatctggaga
     1561 cgctgcgcca cccgctcgtg catggagtcg tcgaagagcg cgacgcagtt ccggccggcg
     1621 tccttcgccc ggtacatggc gatgtcggcg ttgcggtgca actcctcggc gctgacgctg
     1681 ccgttcgtct tcgccacggc caccccgatg ctggcggtga tgtacatgtc gcccgagccg
     1741 acgtggatcg gctcgtggaa gacggacagc acgagatcgg caaacgcgag ggcttccccc
     1801 atcgtccgca cggtcgcgtc gagcgccatg aactcgtcgc cggagatgcg gccgatacgc
     1861 acgtgcggcg ggaccgcggc gcgcagccgg cggccgagca gccgcagcac ctggtcgccc
     1921 acggcgtgac cgaggctgtc gttgatgttc ttgaaccggt cgacgtcgat gaagagcacg
     1981 gtggggcgca cgtcctcgcg ccaggcgtcg tggagcgctt cagtgatctg ctcaccgagg
     2041 agggctcgat tcggcaaccc cgtgagtgcg tcgtgctgcg cggtcaggag cagcgcccgc
     2101 tgggctcg
//
LOCUS       DATDBZ010000539         2703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1196185, whole genome shotgun sequence.
ACCESSION   DATDBZ010000539 DATDBZ010000000
VERSION     DATDBZ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196185"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VIX62_12450"
     CDS             <1..214
                     /locus_tag="VIX62_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861089.1"
                     /translation="AEPTIEPSLIGGLGMGVHGNPPSSVRGYARPMAWTERYVALMDS
                     RPEDRQVVGTPSLPRRGGFINVERIG"
     gene            complement(426..1010)
                     /locus_tag="VIX62_12455"
                     /pseudo
     CDS             complement(426..1010)
                     /locus_tag="VIX62_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544931.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
     gene            1104..1490
                     /locus_tag="VIX62_12460"
     CDS             1104..1490
                     /locus_tag="VIX62_12460"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5861090.1"
                     /translation="MVAEPIRRQVTLDAPPEQVWEAVTTAEHLEAWFGAGVQLDARPG
                     APITVAWPDGTKSRGLVERVEPPHRFVFRWRSMSGAGLTLTVGAPTRVEFTLEPLDDG
                     ARTLVTVTEHDAPLPGPLSSVMSATA"
     gene            1501..1821
                     /locus_tag="VIX62_12465"
     CDS             1501..1821
                     /locus_tag="VIX62_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5861091.1"
                     /translation="MTDEPVDRVFSALSDPTRRAVIRCLSEGGPATLGSLADELPVTR
                     QAVAKHLAQLEDAGLVSASADGRRRTYRLTPGPLTDAMGWMVDVGGDWDERLGALKRH
                     VEHR"
     gene            complement(1839..2255)
                     /locus_tag="VIX62_12470"
     CDS             complement(1839..2255)
                     /locus_tag="VIX62_12470"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEY5861092.1"
                     /translation="MYPPVGTWQALWRGFRKRCPRCGEHDLFVSWFVLRTACPRCEWR
                     FEKEEGGYLGAMTINYTGAILLWIVVLIVGLVLTVPDVPVWPLLAASAVVLIGVPLLF
                     WPRSKAIWAAVEYLALRTDPDYRAPVRRDPRARDLE"
     gene            complement(2305..2667)
                     /locus_tag="VIX62_12475"
     CDS             complement(2305..2667)
                     /locus_tag="VIX62_12475"
                     /inference="COORDINATES: protein motif:HMM:NF018008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5861093.1"
                     /translation="MSETMHDTTIRVVPSNERGDWNPLVDPPGQIPSGGRDLDVFSSA
                     GGTLTTGFWEREPDTWSFERPYDEVALILEGDADIVTDDGRTLTVGPGDVLVTPKGSA
                     GTWVIREKIAKFWVIYDG"
BASE COUNT          423 a          972 c          887 g          421 t
ORIGIN      
        1 ggctgagccc acgatcgagc cttcgctgat cggtggtctt ggcatgggcg tccacgggaa
       61 ccctccttcc tcggtgcgcg ggtacgcgcg accgatggcg tggaccgagc gctatgtcgc
      121 gctcatggac tctcgccccg aggaccgtca ggtggtcggg acgccttctc ttccgcgccg
      181 cggaggtttc atcaacgtag aacgcatcgg ctgagagtgc aaatccagaa cggccttctt
      241 cgtcaaggag attgaccatg tcgcgttcgg cacgacgccg tcgccgcggg cgcgacgtcg
      301 ggcggtggtc aggctcggtc cgcgatgatg ggagcctgct cgccctccgg tgtcgcaggc
      361 aacggaaccg cgaggggggc gccggtcgcg cccagcgccg gcaacccggc gcccagcgcc
      421 tcgagctggc gcacggtcac cacttcgtcc acgacgccgt aggccttcgc ttcctcggcg
      481 gtcaggatga agtcgcggtc ggtgtcggcg ctgatcttct cgacgggctg gcccgtgtgt
      541 tcggccagga tctcgtcggc gcggcggcgc atgcgcacga tctcgcgcgc gtggatctcc
      601 aggtcggccg actgcccctc catcccgccg tgcggctgat gcagcaacac gcgtgagttc
      661 ggcaaggcgt agcgccgccc cgcggctcct gtcgccagca ggaacgcgcc ggcgctcgcg
      721 gcctgcccca cgcagtaggt ggccaccggg gcgtgcagga actgcatcgt gtcgtagatg
      781 ccgaacatcg ccgtggtcga tccgcccgga gagttgatgt acatcgcgat ctcgcgatcg
      841 gggtcctcgg cctgcaggaa cagcagctgg gccacgatca ggttcgccac gttgtcgtcg
      901 atcggcgttc ccaggaccac gatacgctcg cgcagcaacc gcgagtagat gtcgaacgcg
      961 cgctccccac gttcgctcgg ctccaccacc gtggggatca gcgcgtccat ccttgactgc
     1021 accatcatct gctcctctcg cgagttcgca accattcggt tgccaacatc acggtacgcg
     1081 cgatggagta ccatggcaac cacatggttg ccgaaccgat ccgacgccag gtcacgctcg
     1141 acgctccgcc cgaacaggtg tgggaagcgg tcaccacggc cgaacatctc gaggcctggt
     1201 tcggcgccgg cgtgcagctc gatgctcggc ctggcgcgcc gatcacggtg gcctggcccg
     1261 atgggacgaa gagccgcggt ctggtcgaac gcgtcgagcc gccgcatcgg ttcgtcttcc
     1321 gatggcgttc gatgtcgggc gcggggctca cgctcaccgt cggcgctccc acccgcgtgg
     1381 agttcacgct ggaaccgctc gacgacggtg cacgcacgct ggtcacggtc acggagcacg
     1441 acgcgccgct ccccgggcca ctcagctccg tgatgtcggc gacggcatga ggtcgcacgc
     1501 atgaccgacg aacccgtcga ccgcgtgttc tcggcattgt ccgatcccac tcggcgcgcg
     1561 gtgatccgct gcctgtccga aggcggtccc gccacgctcg gctcgctcgc cgacgaactc
     1621 cccgtcacgc gccaggccgt cgcgaagcac ctggcccaac tcgaagatgc cggtctcgtg
     1681 tcggccagtg cggacggccg gcggaggacc taccggctca cgcccggccc gctcaccgat
     1741 gcgatgggat ggatggtcga cgtcggcggc gactgggacg aacggctcgg cgccttgaag
     1801 cgccacgtcg agcaccgctg accaacaccc actagccgtc actccagatc gcgcgcccgc
     1861 ggatcccggc gaaccggcgc gcggtagtcg gggtcggtgc gcagcgccaa gtactcgacg
     1921 gcggcccaga tcgctttcga gcgcggccag aacaacagcg gcacgccgat caacaccacg
     1981 gcgctcgccg cgagcaacgg ccacaccggc acgtcgggca ccgtcagtac cagcccgacg
     2041 atcaacacca cgatccacaa caggatcgcc ccggtgtagt tgatcgtcat cgcgccgagg
     2101 tagccgccct cttccttctc gaaccgccac tcgcaccgag ggcatgcggt gcgcaacacg
     2161 aaccacgaca cgaacaggtc gtgctcgccg caccggggac aacgcttgcg gaacccccgc
     2221 cagagcgcct gccaggtgcc gaccgggggg tacaacggct cgccccggcc accgccgggc
     2281 tcgggttcga gcgagccgta ggggttatcc gtcgtagatc acccagaact tcgcgatctt
     2341 ctcccggatc acccacgtgc cggcggagcc cttgggggtg accagaacgt cgcctggccc
     2401 gaccgtcagg gtccggccgt cgtcggtaac gatgtccgcg tcgccctcca ggatcagggc
     2461 cacctcgtcg tagggacgtt cgaaggacca ggtgtcgggc tcgcgctccc agaagccggt
     2521 cgtgagcgtc ccgcccgcgc tggagaacac gtcgaggtcg cgtccacctg aagggatctg
     2581 ccccggcggg tcgaccaagg gattccagtc gccgcgttcg ttggaaggga ccactcggat
     2641 cgtcgtgtcg tgcatcgtct cgctcatccc cccaggctac cccctcgcct ccgcacgggg
     2701 gat
//
LOCUS       DATDBZ010000540         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2230172, whole genome shotgun sequence.
ACCESSION   DATDBZ010000540 DATDBZ010000000
VERSION     DATDBZ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2230172"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /gene="trpA"
                     /locus_tag="VIX62_12480"
     CDS             complement(<1..464)
                     /gene="trpA"
                     /locus_tag="VIX62_12480"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY5861094.1"
                     /translation="MGQLETALREVRATGGHAFVPYVTGGFPGVDAGLLRALQEAGAD
                     AVEVGIPFSDPVMDGGVVQEASRRSLEAGTTPGAVFDVIAQAGLSIPVAVMTYVNPVM
                     RLGLPAFLDRAREAGVRGVIVPDVPVDEADELCEESSARDIDAVLLAAPGSTA"
     gene            complement(464..1678)
                     /gene="trpB"
                     /locus_tag="VIX62_12485"
     CDS             complement(464..1678)
                     /gene="trpB"
                     /locus_tag="VIX62_12485"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY5861095.1"
                     /translation="MSSSSAAPAGMSASVPDGRGRFGDYGGRYVPEVLIPALDELADA
                     WASLKVDEDFLNELGALSRDFVGRPTPITKAQRLSDELGYEIWLKREDLAHTGAHKIN
                     NALGQALIATRLGKERIIAETGAGQHGVASATACALLGLPCVVYMGEEDIRRQAPNVA
                     RMKLLGAEVVPVTAGTRTLKEAVNEALRDWAGSVNTTHYIIGSVVGPHPFPVLVRDLQ
                     RVIGDESRVQYADAVGADPEYVLACVGGGSNAIGMFTAFVGAPGVELIGVEAGGRGPG
                     LGEHCSSITQGQPGVLHGAMSYLLQDEAGQVVETHSISAGLDYPGVGPEHAWLHDAGL
                     ARYVSATDADALEGFQALARTEGILPALEPSHVIGWLLKRPLPAGARILVCLSGRGDK
                     DLDTVLAALGGS"
     gene            complement(1675..>2510)
                     /locus_tag="VIX62_12490"
     CDS             complement(1675..>2510)
                     /locus_tag="VIX62_12490"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY5861096.1"
                     /translation="RGCLARLGGSMGFLTDLTTQLRRDLADHPLDEGALMARATARPP
                     ARDFEGALRDSHGRPAIIAEVKRASPSAGSIAEATDPVAQASAYEEGGAAAVSVLTEP
                     DHFHGSLLDLEAVRAMVQLPVMRKDFLVHPAQLIEARAHGADAVLLITSSCSDAELTS
                     LLAMADDLGLGVVLETHSDEDLVRASATDASIIGVNARNLETLEVDVPGALSRLEGIA
                     RERVAVMESGIATRRDALAAVAAGASAILVGEALMRAADPSAMVRELTIRNDDRKGST
                     S"
BASE COUNT          414 a          892 c          829 g          375 t
ORIGIN      
        1 gcggtggaac cgggcgcggc gagcaggacg gcatcgatat ccctggccga tgactcttcg
       61 cagagttcgt ccgcttcatc caccgggacg tccggcacga tgacgccacg aacccccgcc
      121 tcgcgagccc gatcgaggaa cgcggggagg ccgagccgca tgaccgggtt cacataggtc
      181 atcacggcga ccgggatgga caaccccgcc tgggcgatga cgtcgaacac cgcacctggt
      241 gtggtgcctg cctccaggga acggcgagac gcctcctgaa ccacccctcc gtccatgacc
      301 ggatctgaga acgggatgcc gacctcgacc gcgtcggcgc ccgcctcctg cagagcccgc
      361 agcaacccgg catccacccc cgggaacccc ccggtgacgt acgggacgaa cgcgtgcccc
      421 cccgtggcgc ggacctcgcg cagcgcggtt tcgagttggc ccatcaagac ccccccagcg
      481 ccgcaagcac cgtatcgagg tccttgtccc ctcgaccgga caggcacacc aggatccgag
      541 cacccgcggg gagcggtcgc ttgagcaacc acccgatcac gtgggacggt tcgagcgccg
      601 gcaggatccc ctccgtccga gcgagtgctt ggaacccttc gagcgcatcg gcgtcggtcg
      661 ccgacacgta gcgggcgagc ccggcgtcgt gcaaccaggc gtgttcggga ccgacccccg
      721 ggtagtcgag tcccgctgag atcgagtgcg tctcgacgac ctgcccagcc tcgtcttgca
      781 ggaggtagga catcgcaccg tgcaggacgc cgggctggcc ctgcgtgatc gaggaacaat
      841 gctcccccag gccgggccca cgccctccgg cttcgacgcc gatcagctcg acgccgggcg
      901 ctccaacgaa ggcggtgaac atgccgatcg cgttgctgcc gcccccgaca catgccagga
      961 cgtactccgg gtcggcgccc acggcatccg catactgcac acgggattcg tcgccgatga
     1021 cgcgctgcag atcgcgaacc agcacgggga acgggtgcgg ccccaccacc gaaccgatga
     1081 tgtagtgcgt ggtgttcacc gacccagccc agtcgcgcaa agcctcgttc acggcctcct
     1141 tgagcgtgcg agtgccggcg gtcaccggca ccacctctgc acccaagagt ttcatgcgag
     1201 cgacgttggg agcctgacgg cggatgtctt cctcgcccat gtacacgaca cagggcaagc
     1261 ccagtagggc gcaggcggtc gcactggcga cgccgtgctg gcccgcccct gtttccgcga
     1321 tgatgcgctc tttgcccagc cgggtcgcga tcagcgcctg cccgagcgcg ttgttgatct
     1381 tgtgcgcgcc ggtgtgggcg agatcctcgc gcttgagcca gatctcgtag ccgagttcgt
     1441 cggagagccg ctgcgccttc gtgataggcg tgggtcgccc cacgaaatca cgtgagagcg
     1501 ccccgagttc attcaggaag tcctcgtcga ccttcaggga ggcccaggca tctgccaact
     1561 cgtcgagggc agggatcagc acctcgggga cgtaccgccc gccgtagtcg ccgaaccgtc
     1621 cgcgtccgtc gggcacggac gcgctcatgc ctgcgggcgc agcgctcgac gagctcatga
     1681 cgtggacccc ttccggtcgt cgttcctgat cgtgagttcc cgtaccatcg cgctcggatc
     1741 ggcggcgcgc atcagtgctt cacccaccag gatggcagaa gctcctgcgg cgacggcggc
     1801 gagcgcgtcg cgacgggtgg cgatgccgct ctccatcacc gccacccgtt cccgcgcgat
     1861 gccctctagg cgcgagaggg cgccgggcac gtccacctca agggtctcga ggtttcgagc
     1921 gttgaccccg atgatcgagg catcggtggc cgacgcacga acgagatcct cgtcgctgtg
     1981 ggtctcgagc acgacaccga ggccgagatc gtcggccatc gcgagcaacg aggtgagttc
     2041 tgcgtccgaa caggacgagg tgatcaacag cacggcgtcg gcgccgtggg cccgagcctc
     2101 gatcagctgc gccgggtgca ccaggaagtc tttccgcatg accggcagtt gcaccatggc
     2161 ccgcacggcc tcgagatcga gcagcgatcc atggaaatgg tcgggttcgg tgaggaccga
     2221 gactgcggca gccccgccct cctcgtacgc gctcgcctga gccaccggat cggttgcttc
     2281 cgcgatcgaa ccggcagacg gggaggcgcg cttgacctcg gcgatgatcg cgggccggcc
     2341 atggctgtcg cgcagcgcac cttcgaaatc gcgcgccggc ggtcgagccg tggcccgcgc
     2401 catgagcgca ccctcatcga gcgggtgatc cgcgaggtcc cggcgcagct gcgttgtcag
     2461 gtcggtgagg aagcccatgg agccgccaag tctagccagg cacccgcgtc
//
LOCUS       DATDBZ010000541         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_165981, whole genome shotgun sequence.
ACCESSION   DATDBZ010000541 DATDBZ010000000
VERSION     DATDBZ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165981"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VIX62_12495"
     CDS             complement(<1..991)
                     /locus_tag="VIX62_12495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5861097.1"
                     /translation="MRGRRLVLTFGLLTALFSAGYGVMFTMLDDFRDEFGIGPTALGA
                     IVATGFFSSFLAQVLLAPLADRGHARRLVYLGMLLNVVGLVGMAVGTTFLTLVLARLV
                     MGIGAGMAQPAIRRIVILSAPDQLGANLGLLLSAEVFGFAMGPALAAILTGPFGIPAP
                     FLVIAVATVLCLPVIARADVQETVDDAAPAARFAFDLLRSRPYVAAVLIGSAVFLMIG
                     TFDALWVLVLSDLNTADWIANLGITLFALPLVVLGSYGGRLAQRVGPYRLGTLGLVGG
                     ATFMFLYGVLPTGGAMFAVAMVHALNDGLTVSSASVAVGLVSPVDRQAGAQGLL"
     gene            complement(1076..2038)
                     /locus_tag="VIX62_12500"
     CDS             complement(1076..2038)
                     /locus_tag="VIX62_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5861098.1"
                     /translation="MEYRPLGRSGLRVSTLSFGSWVTFGNQLDTSLARECLTAARDAG
                     VNFFDNAEVYAGGESERIMGTAIAELGWPRHEYIISTKFFWGIHDGLVNMTNTLNRKY
                     LMQAIDGSLERMGLDFVDLVYCHRADPKTPIEETVRAMSDMVSSGKALYWGTSEWTAD
                     EIRAAWLIADRHHLHKPVVEQPQYNMFERRKVEREYARLYEDLGIGLTTWSPLASGLL
                     SGKYVDGIPEGSRASLPGYEWLRDELTNPKRNESVRKLATIAERLECSLSQLAIAWCA
                     ANPNVSTVITGASRVEQVQENMGAMAVVARLTPDVLDEIRSTLR"
     gene            2166..>2459
                     /locus_tag="VIX62_12505"
     CDS             2166..>2459
                     /locus_tag="VIX62_12505"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEY5861099.1"
                     /translation="MKRWRRRLTGGAIDDAAAADASIRAVVAQRLPAWQHLDDAERDR
                     LVDITIELVREVRWEAARGFELTDEMVLTIAAHAALPVLELGAERYRDVSTIIV"
BASE COUNT          439 a          824 c          841 g          355 t
ORIGIN      
        1 cgagcaagcc ttgtgcacca gcctggcgat cgacaggcga cacgagaccg acggccacgc
       61 tcgcactcga aacggtcagc ccatcgttca gggcatgcac catcgccact gcgaacatcg
      121 cgccacccgt cggcaggacc ccgtacagga acatgaaggt cgcgccaccc accaatccga
      181 gcgttccaag ccggtacggc cccacccgct gggccaggcg gccgccgtac gagccgagca
      241 cgaccaacgg aagcgcgaag agcgtgatgc cgaggttcgc gatccagtcc gccgtgttca
      301 gatcgctcag cacgagcacc cagagcgcat cgaaggtacc gatcatcaag aagacagcgg
      361 agccgatcag cactgcggcc acgtacggcc gcgaccgcaa caggtcgaac gcgaaccggg
      421 ccgccggcgc ggcgtcgtca acggtctcct gcacgtcagc tcgggcgatg accggcaggc
      481 acaggacggt cgcgacggcg atcacgagga acggcgccgg gatgccgaac gggccggtga
      541 ggatggcggc cagggccggg cccatggcga acccgaagac ctcggccgac agcagcaggc
      601 cgaggttcgc tccaagttga tctggcgcag agaggatcac gatgcggcgg atggcgggct
      661 gggccatccc cgcgccgatg cccatcacca gccgggccag gacgagcgtc aggaaggtgg
      721 tgcccaccgc catgccgacg aggcccacca cgttgagcaa catgccgagg tagacgagcc
      781 ggcgggcatg gccgcgatcg gcgagcggcg ccagcaggac ctgggcgagg aacgacgaga
      841 agaatccggt ggcgacgatc gcgccgagcg cagtcggtcc gatgccgaac tcgtcccgaa
      901 agtcgtcgag catcgtgaac atgacgccgt agccggcgga gaacagcgcg gtcaggaggc
      961 cgaacgtgag aacgagccgg cgaccgcgca tgggccacga cggtaccaac gctcctgaag
     1021 tgaacctccg tcgattctcg tgcggcgcca accgccgaga tggcgcccta cggcgtcagc
     1081 ggagcgtgct gcggatctcg tcgaggacgt cgggggtgag ccgggcaaca acagccatcg
     1141 cgcccatgtt ctcctggacc tgctcgaccc tgctcgcacc ggtgatgacg gtcgacacgt
     1201 tcgggttcgc cgcgcaccac gcgatcgcga gttgcgacag ggaacattcg aggcgctcgg
     1261 cgatggtggc gagcttgcgg acgctctcgt tgcgcttcgg gttcgtcagt tcgtcacgca
     1321 gccactcgta gccgggcagc gaggcccggc tgccctccgg gatcccgtcg acgtacttgc
     1381 ccgagagcag gccggaggcg agtgggctcc acgtggtcag tccgatgccg aggtcctcgt
     1441 acaaccgtgc gtactcccgc tcgaccttgc gccgctcgaa catgttgtac tgcggctgct
     1501 ccaccacggg cttgtgcagg tgatgacgat cggcgatcag ccatgcggcc cggatctcat
     1561 cggcggtcca ctcggacgtg ccccagtaga gcgccttgcc cgacgacacc atgtcggaca
     1621 tcgcccgcac ggtctcctcg atcggggtct tcgggtcggc ccggtggcag tacacgagat
     1681 cgacgaagtc gagtcccatg cgttccagcg aaccgtcgat ggcctgcatc aggtacttcc
     1741 ggttgagggt gttcgtcatg ttgacgaggc catcgtggat gccccagaag aacttcgtcg
     1801 agatgatgta ctcgtgccgg ggccagccca gctcggcgat ggcggtgccc atgatccgct
     1861 cggactcgcc gccggcgtac acctcggcgt tgtcgaagaa gttgaccccg gcgtcgcggg
     1921 cagcggtcag gcattcgcgg gcgaggctgg tgtcgagttg gttcccgaac gtgacccacg
     1981 acccgaagga gagcgtgctc actcgcaggc cggagcgccc gagggggcgg tactccatcg
     2041 ttgcggtcgt gtcatctgcc atagcgtctt ctcccgggtt cgacgagttc tccgagggcg
     2101 acggtaccgc cgctccccac cgtcgatagc gtccgaccgg tgatcaatcg ggctgaggtc
     2161 gagtcgtgaa gcggtggcga cgccggctca cggggggcgc gatcgacgac gccgcggccg
     2221 ccgacgcgtc gatccgggcc gtggtcgcgc agcgattacc ggcctggcag cacctcgacg
     2281 acgccgagcg ggaccgcctc gtcgacatca ccatcgagct cgttcgggag gtgcgctggg
     2341 aagccgcacg cggcttcgag ctcaccgacg agatggtgct aacgattgcc gcccacgccg
     2401 cactgcccgt gctcgagctc ggcgcagagc ggtaccgtga cgtctccacg atcatcgtg
//
LOCUS       DATDBZ010000542         3004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2007742, whole genome shotgun sequence.
ACCESSION   DATDBZ010000542 DATDBZ010000000
VERSION     DATDBZ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007742"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VIX62_12510"
     CDS             <1..135
                     /locus_tag="VIX62_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY5861100.1"
                     /translation="AAPAPAAAAGGDMMAQLQQLAAMNAQGVLTDEEFAAAKARLLAG
                     "
     gene            complement(184..1080)
                     /locus_tag="VIX62_12515"
     CDS             complement(184..1080)
                     /locus_tag="VIX62_12515"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HEY5861101.1"
                     /translation="MLHLSAFVKPDSTSAVCDGLSAIPGVRRVMVGSVTTDGFVNITA
                     HLEAGSADAAIDLLAHHDVRADDVGLLRLPVLLPLLNRRGARDPDAQVWAEVVGRAWG
                     NSHLKPTDLVFMIAAGVIAGVGVLTGSSILVVGAMAISPDLLPITASAVGLVEREWRL
                     AARAGRSLVGGLSTSAVAACVAILILRATDRLGDDIALSESVLGESLTTLGPGTALVA
                     IAAGVAGMLAYERLGGAAVGVAISVTTIPAAAYVGVGVALGRGTPVWGALLVLVTNVV
                     LVVAASAATLWVQRTNQRRRSA"
     gene            1194..>3004
                     /locus_tag="VIX62_12520"
     CDS             1194..>3004
                     /locus_tag="VIX62_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY5861102.1"
                     /translation="MPAVEHADAGHQKSLLPLAFGALGIVYGDIGTSPLYAFRESFEH
                     ADLAVTQANAYGVASVVFWALIVIISIKYLMLVMRADNHGEGGILALTALLMPARGLP
                     VGTKRAIIALGVFGTALLYGDGLITPAISVLSAVEGFEVATTAFESWVIPVSVAILVV
                     LFVVQKRGTETISRVFGPVMVVWFIVLGVLGLRQIIDNVGVLRAVSPTYGIALFVDQP
                     AHAFLALGSIFLVVTGGEALYADMGHFGRRPIQLSWYGLVLPALLLNYFGQAALLAGS
                     PGEEVGNPFFRMAPSWAVVPLAVLATMATVIASQALISGAFSLTAQAVQLDYLPRLAI
                     RHTSSAHIGQIYVPLVNWLLMIGCVGLVIGFQTSSNLAAAYGIAVTATMAITTLLFFR
                     VVRDRWGWSARKALAVVVPLFLVDVAFFAANVPKIPDGGWLPILVGLGLVIQMTTWRR
                     GRAIVSQILQRGHRRTVDVIAEAVAGGVTKVPGTAIYMFKDPGFAPPAMISNLRHNHV
                     LHETTLMLSVIASQSPSVEPAERTSLTTIAPGIYELVITFGYMDEHDVMAELRQLRLD
                     GRPIDLDDATFFIGRETVSSIPEGEMPRWREELFIVL"
BASE COUNT          475 a         1067 c          957 g          505 t
ORIGIN      
        1 gcggctccgg ctcccgctgc cgctgccggt ggcgacatga tggcccagct gcagcagctc
       61 gccgccatga atgcccaggg tgtgctcacc gacgaggagt tcgccgccgc gaaggcacgt
      121 ctgctcgccg gttgacgatc aactgatcgg cagcaccgcg cgacccgccc aagtcgtctc
      181 gcgtcaggct gaccgccggc gttgattcgt gcgctggacc cacaaggtcg ccgcgctcgc
      241 cgccacgacc agcaccacgt tggtcaccag cacgagcagc gcgccccaca caggcgtccc
      301 acggcccaaa gccaccccga ccccgacgta ggcagcggca ggaatggtgg tgaccgagat
      361 cgccacgccg accgcagcac caccgagccg ttcatacgcc agcatccccg ccacgccagc
      421 ggcgatggcg accagcgccg tgccgggacc gagcgtggtg agtgactccc cgagcaccga
      481 ctcactgagc gcgatgtcgt ccccgaggcg gtcggtggcg cgcaggatca agatggcgac
      541 gcacgcggcc actgcgctcg tcgacagacc acccaccagt gaccgtcccg cccgagcggc
      601 gagccgccac tcgcgctcga cgagaccgac tgcgctcgcg gtgatcggca gcaggtcggg
      661 gctgatcgcc atggcgccga cgacgaggat cgacgatccg gtcagcacgc ccacgcccgc
      721 gatcacaccg gccgcgatca tgaacaccag atcggtgggc ttgaggtgcg agttccccca
      781 cgccctgccg accacctcgg cccacacctg ggcatcggga tccctcgcgc cgcgccgatt
      841 cagcagcggc aagagcacgg gtagccgcag gaggccgacg tcgtcggcgc gtacgtcatg
      901 gtgggcgaga aggtcgatcg cggcatcggc cgagcccgcc tccaagtgcg ccgtgatgtt
      961 cacgaaaccg tcggtggtga ccgatccgac catcacccga cggacacccg ggatcgccga
     1021 cagaccgtcg cacaccgcgg acgtcgagtc gggcttcacg aaagcgctca gatgcagcat
     1081 cgtcgggcac cttcactcgc gtccgcgatc atcgaatcgc agcgtagcca gcacctctgg
     1141 cggcaacggc ctcgtccgtg tcaagcaccc gggagccgga tagcttgccc atcgtgcccg
     1201 ccgtcgagca tgccgatgcc ggccaccaga agagcttgct gccactcgcg ttcggcgccc
     1261 tcggcatcgt gtacggcgac atcggtacga gcccgttgta tgcgttccgg gaatcgttcg
     1321 agcacgccga cctggcggtg acgcaggcca atgcgtacgg agtggcctcg gtggtgttct
     1381 gggcgctgat cgtcatcatc tcgatcaagt acctgatgct cgtgatgcgg gccgacaacc
     1441 acggcgaggg cggcattctt gcgctgaccg cgctgctgat gcccgcacgc gggctaccgg
     1501 tcggaacgaa gcgcgccatc atcgcactcg gtgtgttcgg caccgccctg ctgtacgggg
     1561 atggcttgat cactccggcc atctcggtgc tcagtgctgt cgaggggttc gaggtcgcca
     1621 cgactgcgtt cgagtcgtgg gtgatccccg tatcggtggc catcctggtc gtgttgttcg
     1681 tcgttcagaa gcgcggcacc gaaacgatct cgagggtgtt cggtccggtg atggtggtgt
     1741 ggttcatcgt gctcggcgtg ctcgggctcc gccagatcat cgacaacgtc ggcgtgctcc
     1801 gggccgtgtc acccacctac ggcatcgccc tcttcgtcga ccaaccggcc cacgcgttcc
     1861 tcgcgctggg ctcgatcttc ctggtggtga cgggcggtga agccctctac gccgacatgg
     1921 gccacttcgg tcgccgaccg atccagctct cctggtacgg cctggtgctc ccggccttgc
     1981 tgctcaacta cttcggccag gcagccctgc tcgcaggcag cccgggcgaa gaggtgggca
     2041 acccgttctt ccggatggcg ccgtcgtggg cggtcgtgcc cctcgccgtg ttggcgacga
     2101 tggccacggt catcgcgtcg caggcgttga tctcgggtgc gttctcgctc accgctcaag
     2161 cggtgcagct cgactacttg ccacgactgg cgatccgcca cacctcctcg gcccacatcg
     2221 ggcagatcta cgtgccgctc gtcaactggc tgctcatgat cggatgcgtc gggctcgtga
     2281 tcggcttcca gacatcgagc aacctggccg cggcatacgg catcgccgtg accgccacga
     2341 tggccatcac caccctcctg ttcttccgcg tcgtccgcga ccggtggggt tggagcgcac
     2401 gcaaggcgct cgccgtggtc gtgccgctgt tcctggtcga cgtcgccttc ttcgccgcca
     2461 acgttcccaa gatccccgat gggggctggc tcccgattct cgtcggcctc ggcctggtga
     2521 tccagatgac gacctggcga cgaggacggg ccatcgtgtc gcagatcctc caacgtggtc
     2581 atcgccgaac cgtggatgtg atcgcggagg ccgtcgccgg cggcgtcacc aaggtgcccg
     2641 gcacggccat ctacatgttc aaggacccag gcttcgctcc gccggcgatg atctccaacc
     2701 ttcgtcacaa ccacgtgctc cacgagacga cgctgatgct gtcggtgatc gcgtcgcagt
     2761 cgccgagcgt cgagcccgct gagcgcacgt ccctcacgac gatcgcccct ggcatctacg
     2821 agctggtcat cacgttcgga tacatggacg aacacgatgt gatggccgag ctgcgccagc
     2881 tccgcctcga cggtcgcccg atcgacctcg acgacgccac cttcttcatc ggccgggaga
     2941 cggtgtcgtc gatccccgaa ggcgagatgc cgagatggcg cgaggagctc ttcatcgtcc
     3001 tgaa
//
LOCUS       DATDBZ010000543         4742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2008372, whole genome shotgun sequence.
ACCESSION   DATDBZ010000543 DATDBZ010000000
VERSION     DATDBZ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2008372"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VIX62_12525"
     CDS             <1..288
                     /locus_tag="VIX62_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5861103.1"
                     /translation="SNTHLAQRFKDLIGVTPKRLARNYRFATTVLSINPAGPIDWGDL
                     AGGAGYFDQAHFGHEFRAFTGLTPTRYVEIRRRFLREHPGHALDSWPRPAD"
     gene            354..934
                     /locus_tag="VIX62_12530"
                     /pseudo
     CDS             354..934
                     /locus_tag="VIX62_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
     gene            complement(994..2010)
                     /locus_tag="VIX62_12535"
     CDS             complement(994..2010)
                     /locus_tag="VIX62_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEY5861104.1"
                     /translation="MDIGVVLQTTPPSARVVDLAKRAETYGFSHVWTFDSHLLWEEPF
                     PIFTQILAETRTVTVGPMVTNPLTRDWTVTASLFATLNEMFGNRTVIGIGRGDSAVRV
                     INGKPSTLRALGESVHVIRELVNGREVEYRGSTLRLPWASKSRAQVWMAGYGPKALRL
                     IGEVGDGFILQLADPDIAAWTIGAVRRAAKEAGRDPAELTICVAAPAFVGDDLAYARD
                     QCRWFGGMVGNHVADIVARYGETSDVPAALTDYIRGREGYDYNEHGQVGNVHTAFVPD
                     EIVDRFCILGPVTEHVRRLQELHALGVDQFAVYLQHDAKDSTLQAYGEKVLPTLAEHV
                     AART"
     gene            complement(2021..3445)
                     /gene="hydA"
                     /locus_tag="VIX62_12540"
     CDS             complement(2021..3445)
                     /gene="hydA"
                     /locus_tag="VIX62_12540"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008714992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY5861105.1"
                     /translation="MARTLIKNGTVISPAGVEPADVLIDGETIASVGAPGFFADAEPG
                     CEKVIDATEKHVVPGGIDVHTHMELPFGGTFASDTFETGTRAAAWGGVTTIIDFAVQR
                     TGEDVQAGLAEWHRKAEGECAIDYGFHQIIGGVDDAALKSMKYLTEHEGVTSFKLFMA
                     YPGVLYSDDGQILRAMQTAAECGAMIMMHAENGIAIDVLVAQALARGDTDPVHHGYTR
                     PSALEAEATHRAIVLADVAGNVPLYVVHMSAADALEEIAVARRDGRNVFAETCPQYLW
                     LTLEETLARPGFEGAKWVCSTPLRSSTDGRHHQENLWQGLRTNDLAIVSTDHCPFCFK
                     DQKELGLGDFSKIPNGIGGVEHRMELMYQGVVTGQLSLERWVETCCTTPARMFGMYPR
                     KGIIAPGADADVLVWDPDRQTTIGIDDKHHMNMDYSAYEGFVVDGKVDTVLSRGTVVI
                     EDDAYVGRKGHGRFIARELSQYLV"
     gene            complement(3474..4316)
                     /locus_tag="VIX62_12545"
     CDS             complement(3474..4316)
                     /locus_tag="VIX62_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY5861106.1"
                     /translation="MSTTVKAALLQTDWAGTQEAMIDKHEEAARGAAAQGAQVICFQE
                     LFYGPYFCQVQDAQYYDYTEPIPDGPTTKRFQSIAKELGMVMVLPMYEIVQPGLYHNT
                     AAVIDADGTYLGKYRKQHIPQVQGFWEKFYFAPGTGGYPIFETAVGKVGVYICYDRHF
                     PEGWRMLGLGGAEIVFNPSATHRGLSEYLWRLEQPAAAVANMYYVGAINRVGIEPLGE
                     NDFYGQSYFVDPEGQFVGEVGDAFKPELIVRDLDLGKIREVRDRWAFYRDRRPDAYDA
                     IVAR"
     gene            complement(4483..>4742)
                     /locus_tag="VIX62_12550"
     CDS             complement(4483..>4742)
                     /locus_tag="VIX62_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HEY5861107.1"
                     /translation="GHEHNYERFAAQDAFGNAAADGMTEIVAGTGGNGEGSYPFGNPI
                     PNSLVRLNGLGLVELRLWDGAWLERFHGEDGEVLDRTSGDC"
BASE COUNT          726 a         1637 c         1555 g          824 t
ORIGIN      
        1 agcaacactc acctggcaca gcggttcaag gacctcatcg gcgtcacgcc gaagcggctg
       61 gcccgcaact accgcttcgc caccaccgtg ctctcgatca accccgccgg accgatcgac
      121 tggggcgacc tcgccggtgg cgcaggctac ttcgaccagg cccacttcgg ccacgagttc
      181 cgggcgttca ccgggctcac gccgacccgg tacgtcgaaa tccggcggcg gttcctgcgc
      241 gaacatcccg gccacgcgct ggacagctgg ccgcggccgg ccgattgatc tcttacaaga
      301 gcgacagctc acgacactct aatttggggc accccaaagc aaaggagagc cccgtgggca
      361 aggtggtcat gtacagctcg gtgtcggtgg acggcttcgt cgcggacgag agcgaccagc
      421 ccggaccgct gttcgactgg ttgtccagcg gtgacgtccc gttggacgag agcggcgagg
      481 tgaaggtgtc gcagacgtcc tacgactaca tccggccgta ctgggaccag atcggggcga
      541 cagtcgtcgg ccgccacgtc ttcgacatga cggacggctg ggacgggaag cctccgggcg
      601 ggatcgacca cgtggtcgtc gtgacgcacc gaccggagcc cgagggctgg gaccccgagg
      661 cccgtttcac ttcgtcgagg gcgtcgaggc agccgtggcc aaggcgcagg agcttgcggg
      721 tgaccgcttg gtcgaggtcg ctgctggcga cgtcggtggc caggtgcttg ccgcgggtct
      781 gatcgacgag gtgcgcatgg acgtcgtacc cgtcgtgctc gggtccggca agcgctactt
      841 cgggtcggtc cacgcgcagc acctgttgga ggatcctgac gtggtgattc agggcaaccg
      901 ggtgcttcac ctgcgctatc gggtgcgccg ttgactgtct gcctgcacct cgctcccggc
      961 cacgcctgcc acctctcccc cgctcggtct ggctcaggtc ctcgcggcga cgtgctcggc
     1021 gagcgtcggc aggaccttct ccccgtacgc ctggagcgtc gagtccttgg cgtcgtgctg
     1081 taggtacact gcgaactggt ccacacccag cgcgtgcagc tcctggaggc gcctcacgtg
     1141 ctcggtcaca gggcccagga tgcagaagcg gtccacgatc tcgtcgggca cgaacgcggt
     1201 gtggacgttg ccgacctgtc cgtgctcgtt gtagtcgtac ccctcgcgtc ccctgatgta
     1261 gtcggtcagt gccgcgggaa cgtccgaggt ctcgccgtag cgcgccacga tgtcggcgac
     1321 gtggttgccc accatgccgc cgaaccatcg gcactggtcg cgcgcgtacg cgaggtcgtc
     1381 ccccacgaag gccggcgccg cgacgcagat cgtgagttcg gctggatcgc ggcccgcctc
     1441 cttcgcggcc cgccgaacgg cgccgatcgt ccacgccgcg atgtcggggt cggcgagctg
     1501 gaggatgaac ccgtcgccca cctcgccgat cagccgcagg gccttgggtc cgtacccggc
     1561 catccacact tgcgctctgc tctttgacgc ccacggcagc cgcagcgtcg agccccgata
     1621 ctcgacctca cgtccgttta cgagctcgcg gatcacatgg acggactcgc ccagcgcccg
     1681 caatgtcgac ggcttcccgt tgatcacgcg caccgccgag tcgccccggc cgatgccgat
     1741 caccgttcgg ttgccgaaca tctcgttcag cgtggcgaag aggctggccg tgaccgtcca
     1801 gtcgcgcgtg agcgggttgg tgaccatcgg gccgacggtg acggtgcgcg tctcagccag
     1861 gatctgcgtg aagatgggga acggctcctc ccacaacagg tgactgtcga acgtccacac
     1921 gtgactgaac ccgtaggtct cggcccgctt cgcgaggtcc acgacccgtg cgctcggcgg
     1981 tgtcgtctgc agcaccacgc cgatgtccat ggccttcctc ctatacgagg tactgcgaca
     2041 gctcgcgagc gatgaatcgg ccgtgaccct tgcggccaac gtaggcatcg tcctcgatca
     2101 ccacggtgcc gcgcgagagc acggtgtcga ccttgccgtc gaccacgaag ccctcgtagg
     2161 ccgagtagtc catgttcatg tggtgcttgt cgtcgatgcc gatcgtggtc tgccgatccg
     2221 ggtcccacac gagcacgtcc gcgtcggcgc ccggcgcgat gatccccttg cgcgggtaca
     2281 tgccgaacat ccgcgccggc gtcgtgcagc acgtctcgac ccaccgttcc agcgagagct
     2341 gacccgtcac gacgccctgg tacatcagct ccatgcggtg ctcgaccccg ccgatgccgt
     2401 tcgggatctt ggagaagtcg cccaggccca gctccttctg gtccttgaag cagaacgggc
     2461 agtggtcggt ggagacgatc gccaggtcgt tcgtgcgcag cccctgccac aggttctcct
     2521 gatggtggcg cccatcggtc gaggagcgca gcggcgtcga gcacacccac ttcgcgcctt
     2581 cgaacccagg cctggcgagc gtctcctcca gcgtcagcca caggtactgc gggcaggtct
     2641 cggcgaagac gttgcgaccg tcccgccgcg cgacggcgat ctcctcgagc gcgtcggccg
     2701 cggacatgtg caccacgtag aggggcacgt tgcccgcgac gtccgccagc acgatcgcgc
     2761 gatgcgtggc ctcggcctcc aacgcggacg gccgggtgta gccgtggtgc acgggatcgg
     2821 tgtcgccccg agccagcgcc tgggccacga gcacgtcgat cgcgatgccg ttctcggcgt
     2881 gcatcatgat catggcgccg cactccgccg cggtctgcat cgcgcgcagg atctgtccgt
     2941 cgtcgctgta gagcacgcca ggatacgcca tgaacagctt gaagctcgtg acaccctcgt
     3001 gctcggtgag gtacttcatg gacttcagcg ccgcgtcgtc gaccccgccg atgatctggt
     3061 ggaagccgta gtcgatcgcg cactctccct cggccttgcg gtgccactcg gcgaggccgg
     3121 cctgcacgtc ctcgccggtc cgctgcaccg cgaagtcgat gatcgtcgtg acgccgcccc
     3181 acgcggcggc gcgggtgccc gtctcgaacg tatcggaggc gaacgtcccg ccgaacggca
     3241 gctccatgtg ggtgtggacg tcgatcccgc caggaaccac gtgcttctcg gtcgcgtcga
     3301 tgaccttctc gcatcccggc tcggcgtcgg cgaagaagcc gggagcgccg accgaggcga
     3361 tcgtctcgcc gtcgatcagg acgtcggcgg gctcgacccc ggcgggcgag atgaccgtcc
     3421 cgttcttgat cagcgttcgt gccatgtgtc ggacctcctc gcgcgtccgg tgctcagcgc
     3481 gcgacgatcg cgtcgtaggc gtcggggcgg cgatcgcgat agaacgccca gcggtcgcgg
     3541 acctcgcgga tcttcccgag gtcgaggtcc cgcacgatca gctccggctt gaacgcgtcg
     3601 ccgacctcac ccacgaactg tccctccgga tcgacgaagt agctctgccc gtagaagtcg
     3661 ttctcgccga gcggctcgat gcccacgcgg ttgatcgcac ccacgtagta catgttcgcg
     3721 acggccgcgg ccggctgctc gagtcgccac aggtactcgc tgagcccacg gtgcgtggca
     3781 ctggggttga agacgatctc ggcgcctccg aggccgagca tgcgccagcc ctcggggaag
     3841 tggcggtcgt agcagatgta gacgccgacc ttaccgaccg cggtctcgaa gatcggatac
     3901 ccaccggtgc ccggcgcgaa gtagaacttc tcccagaagc cctggacctg cgggatgtgc
     3961 tgcttgcggt acttgccgag ataggtgccg tcggcgtcga tgaccgcggc ggtgttgtgg
     4021 tacaggcccg gctgcacgat ctcgtacatg ggcaggacca tcaccatccc aagttccttc
     4081 gcgatgctct ggaaccgctt cgtggtcggc ccgtccggga tcggctcggt gtagtcgtag
     4141 tactgagcgt cctgcacctg gcagaagtac ggcccgtaga agagctcctg gaagcagatg
     4201 acctgcgcgc cctgagcggc ggcaccgcgg gccgcctcct cgtgcttgtc gatcattgcc
     4261 tcttgggttc cagcccagtc ggtctgcagc agggccgcct tcacggtggt ggacatggca
     4321 tcgctcctcg gtcctcggtt cggccctcgt gcagatatgt cagcacgcga gcgtttcttt
     4381 gcggcatggt agcccgccgc ggcgcggtgc ggttcaaccg acgacgcgga acagattgcc
     4441 ctcagaaccg tgctccgcca cggttcgcct ggggaaccgc tgctagcagt ctcccgaagt
     4501 acggtcgagc acctcgccat cctcaccgtg gaatcgttcc agccatgcgc catcccagag
     4561 gcgaagctcg accagcccga ggccattcaa gcggaccagg gagttgggga tcgggttgcc
     4621 gaacgggtag gagccctcac cgttgccacc ggttccggca acgatctcag tcatcccgtc
     4681 ggcagcagcg ttgccgaacg cgtcctgggc agcgaaccgc tcgtagttgt gctcgtgacc
     4741 at
//
LOCUS       DATDBZ010000544         3423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1627570, whole genome shotgun sequence.
ACCESSION   DATDBZ010000544 DATDBZ010000000
VERSION     DATDBZ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627570"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1987
                     /locus_tag="VIX62_12555"
     CDS             <1..1987
                     /locus_tag="VIX62_12555"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861108.1"
                     /translation="ESATWERQAMIKARPVAGRADLGQGFLRGIEPFVYPPELAPAAI
                     EDVRRTKVRLEEYIRQRGKEFTEVKRGRGGIRDVEFAVQLLQIVHGRRDAKLRTPTTL
                     AALRALAIEGYVGDGDAEALADAYRFLRRLEHRLQIVRDLQTHDLPPDRRSRTTLARS
                     LGQADADALAERYEATTGLVRSIHERLFYRPLLEAFAGPAPMAQLAHGTDRAATEELL
                     GGLGFTDPARSYDVLGRLVDPSHRIGKVLAHLFPVMAPSLALSPVPDSALVRLERVAE
                     AIGRHEDERAIADLLAGDPLAARRLAHVAGGSSFASDLLVVEPTRIRALSDTLLGGAT
                     DPAGELVDAIARSAGRELSPRGTGEALSAVAVRVVRDAVAAAEPDLPLAVIGMGKLGA
                     RELNVASDLDLLFVYEGEGSGDLKRASQAAERVLRDIRDAGWEPDSDLRPEGRNGPLA
                     RSIASYLEYWERYAETWEFQSLLRARAIAGDPDLGRRFELNAADFAFPPDGITIDRVV
                     EIRRMRERIERERVKPPEASKFHFKLGHGSLADVQFAVEISLMRHGGPHPEIRSRRTL
                     EAIDRLAEAKLLEGSVARDLGSAFVFLSDVKNALEMDRRLHVEAVPPGPAEQTALARR
                     LGYQEYPRQSFIDDYLRITRRARRAMERVFTEEVAPA"
     gene            1984..2802
                     /locus_tag="VIX62_12560"
     CDS             1984..2802
                     /locus_tag="VIX62_12560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008187946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5861109.1"
                     /translation="MSTPGTRSTRTGTTRRTALITGASSGFGSLFAERFAADGFDVVL
                     VARSVEPMDTLADRIRSRNAVQVAVIGHDLGEPGAAASLVAELAGRGLTVDALVNNAG
                     FSTYGPFADTGEATTTEMLMVNVVAMTALARACLPGMLERRWGRVLLLGSVGSFSPAP
                     MTAAYGASKAYVLSLGLALHDELKGSGVTVTTLCPGPTATGFQERADMHDSALVRSGL
                     DSAETVVDKGYAAMKAGRPYLVTGTTSRLFAFGTRFLPRTTAAGIAGNAQRRVG"
     gene            2821..3198
                     /gene="mscL"
                     /locus_tag="VIX62_12565"
     CDS             2821..3198
                     /gene="mscL"
                     /locus_tag="VIX62_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY5861110.1"
                     /translation="MLKEFKEFAMKGNLIEVAVGLVLALAFTAVVNALIEGIIMPLIA
                     AIFGKPSFDAIVWTIGSGRDATQILIGTFLTAVVNFLLIAWVLFLIVRAANRFRKPAE
                     EPTGPTEVELLTQIRDSLASLPN"
     gene            complement(3195..>3423)
                     /locus_tag="VIX62_12570"
     CDS             complement(3195..>3423)
                     /locus_tag="VIX62_12570"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY5861111.1"
                     /translation="AVVFTGCNRGSAAVEVSDASLTFGPLRLTRMACGDDATALEATV
                     TSVIDGKVDYSINGDVLTVSKDAQALEYRAG"
BASE COUNT          495 a         1203 c         1203 g          522 t
ORIGIN      
        1 ggaatcggcc acgtgggagc gccaggcgat gatcaaggcc cgtcccgtcg cgggccgggc
       61 cgacctgggc cagggcttcc tgcgggggat cgaaccgttc gtctatccgc ccgagctggc
      121 gcccgcggcg atcgaggacg tgcggcgcac gaaggtccgg ctggaggaat acatccggca
      181 gcggggcaag gagttcacgg aggtcaagcg cggccgtggc ggcatccgcg acgtggagtt
      241 cgcggtgcag ctcctgcaga tcgtgcacgg gcggagagac gcgaagctcc gcacgcccac
      301 cacgctcgcc gccctgcggg cgctcgcgat cgagggctac gtcggtgacg gtgacgccga
      361 ggccctcgcc gacgcgtacc ggttcctgcg acgcctggag caccgtctgc agatcgtgcg
      421 cgacctgcag acccacgacc tgccgcccga ccgccgctca cgcaccaccc ttgcgcgatc
      481 gttgggtcag gccgacgccg atgcgctggc cgagcggtac gaggcgacga ccggactcgt
      541 ccgttcgatc cacgagcggt tgttctaccg gccgttgctg gaagcgttcg ccggaccggc
      601 gccgatggcc cagctcgccc acggcaccga ccgcgccgcc accgaggagc tcctgggcgg
      661 cctcgggttc accgaccctg cgcggtccta cgacgtgctg ggccgcctgg tggatccgtc
      721 gcaccggatc ggcaaggtgt tggcgcacct gttcccggtg atggcgccgt cgctcgccct
      781 ctcgcccgtg cccgactcgg cgctcgtccg gttggagcgg gtcgccgaag ccatcggccg
      841 ccatgaggac gaacgggcga tcgccgatct gctcgccggc gatcccctgg ccgcccgccg
      901 gctcgcgcac gtcgccggcg ggagctcgtt cgcctccgac ctcctcgtgg tcgaacccac
      961 gcggatccgc gcgctgtcgg acacgctgct gggcggtgcc accgacccgg cgggcgaact
     1021 ggtggatgcc atcgcgcgga gcgccggccg cgaactctcc ccgcgcggaa ccggtgaggc
     1081 gctctccgct gtggcggtgc gggtggtgcg cgacgcggtc gcggccgccg agcccgacct
     1141 gccgctcgcc gtgatcggga tgggcaagct gggcgcgcgc gagctcaacg tcgcgagcga
     1201 cctcgacctg ctgttcgtct acgagggcga gggctccggc gacctgaagc gcgcgagtca
     1261 ggccgccgaa cgggtgctgc gcgacatccg cgacgccggg tgggaacccg attcggacct
     1321 gcgcccggag ggacgcaacg ggccgctcgc gcgctcgatc gcctcctacc tggagtactg
     1381 ggagcgctac gccgagacgt gggagttcca gtcgctgctg cgggcccgcg cgatcgccgg
     1441 cgatcctgac ctcggtcgcc gcttcgagtt gaacgccgct gacttcgcct tcccgcccga
     1501 cggcatcacg atcgaccgcg tcgtggagat ccgccgcatg cgggagcgca tcgaacgcga
     1561 acgcgtgaag ccacccgagg cgtcgaagtt ccatttcaag ctgggacacg gatcgctcgc
     1621 cgacgtccag ttcgcggtgg agatctcgct gatgcgccac ggcggcccgc atccggagat
     1681 ccggtcgcgt cgcacactcg aagcgatcga ccggctggcc gaggcgaagt tgctcgaggg
     1741 cagcgtggct cgcgatctgg gctcggcgtt cgtgttcctg tccgacgtga agaacgcgtt
     1801 ggagatggac cgccgcctgc atgtcgaggc ggtgccaccc ggccccgcgg agcagaccgc
     1861 gctggccagg cgcctgggct accaggagta ccctcgacag agtttcatcg acgactacct
     1921 gcgcatcacc cgtcgtgccc gccgggccat ggaacgggtc ttcaccgagg aggtcgcgcc
     1981 cgcatgagca cgcccggaac ccgatccacg cgaaccggca ccacccgtcg cacggccctg
     2041 atcacgggcg cctccagcgg gttcgggtcg ttgttcgccg agcggttcgc ggccgacggg
     2101 ttcgacgtgg tactggtggc gcgcagcgtc gagccgatgg atacgctggc cgaccgcatc
     2161 cgttcgcgca acgccgtcca ggtcgccgtg atcgggcacg acctcggcga acccggcgcc
     2221 gccgcgagcc tcgtcgctga gctcgccggg cgcgggctca cggtcgacgc actggtcaac
     2281 aacgcgggct tctccaccta cgggccgttc gccgacacgg gcgaggccac gacgaccgag
     2341 atgctcatgg tcaacgtcgt ggccatgacc gcactcgcac gcgcgtgtct gccgggcatg
     2401 ctcgagcgcc gatggggtcg cgtgctcctg ctgggctcgg tgggttcgtt cagtcccgcg
     2461 cccatgaccg cggcctacgg agcgtccaag gcctacgtgt tgtcgctcgg gctcgcgctc
     2521 cacgacgagc tcaagggatc gggcgtcacg gtcaccacgc tctgtcccgg ccccacggcg
     2581 accgggttcc aggaacgcgc ggacatgcac gattcggccc tggtccggtc gggcctggac
     2641 tcggcggaaa cggtcgtcga caaggggtac gccgcgatga aagcgggaag gccctacctc
     2701 gtgaccggca ccaccagtcg cctgttcgcg ttcggcacac ggttcttgcc caggaccacg
     2761 gccgccggca tcgccgggaa cgcccagcga cgcgtcggct gatcggcaag gagggacatc
     2821 atgttgaagg agttcaagga attcgccatg aagggcaacc tgatcgaggt cgcggtgggg
     2881 ttggtgctgg ccctggcgtt caccgcggtg gtgaacgcgt tgatcgaggg catcatcatg
     2941 ccgctgatcg ctgcgatctt cggcaagccc agtttcgacg cgatcgtatg gacgatcggc
     3001 tcgggcaggg atgccacgca gatcctgatc ggcacgttcc tcaccgcggt cgtgaacttc
     3061 ctgctgatcg cgtgggtgct gttcctgatc gtgagggcgg cgaaccggtt ccggaagccc
     3121 gccgaggaac cgacgggccc caccgaggtc gagctgctga cgcagatccg tgattcgctc
     3181 gcgagcctgc ccaactagcc cgcgcggtac tccagcgcct gtgcgtcctt ggacacggtg
     3241 aggacgtcgc cgttgatcga gtagtccacc ttgccgtcga tgaccgatgt cacggtcgcc
     3301 tccaaggcgg tcgcgtcgtc gccgcaggcc atcctcgtga ggcggagcgg accgaatgtc
     3361 agactggcgt cgctgacctc gaccgcggcg ctgcctcggt tgcagcccgt gaacaccacg
     3421 gcc
//
LOCUS       DATDBZ010000545         5120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1627726, whole genome shotgun sequence.
ACCESSION   DATDBZ010000545 DATDBZ010000000
VERSION     DATDBZ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627726"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VIX62_12575"
     CDS             <1..212
                     /locus_tag="VIX62_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY5861112.1"
                     /translation="QGELGYVYNAHLSAYGQLGYVHTGDVIGYVGSTGDATAPHDHFE
                     WHPNDGPAVDPNPYLSVSCDGSSPS"
     gene            complement(209..862)
                     /locus_tag="VIX62_12580"
     CDS             complement(209..862)
                     /locus_tag="VIX62_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY5861113.1"
                     /translation="MRRRLIALALIVTGLLALAAPAQARPEFSARVSPLDASTRHLMV
                     GRSWRPGCPVPLRDLRLIRMKIWGFDRRWHRGSLVVHRRYTDEMVRVFKRLYEVRYPI
                     RQMRLVDHFGADDHRSMAADNTSAFNCRYRAGICCTWSQHAYGRALDLNTVENPYVYA
                     GGFSPANGRPFLDRSQHRRGMIHRHDAVWWAFHAVGWSWGGDWSGEKDYQHFSANGR"
     gene            complement(876..1637)
                     /locus_tag="VIX62_12585"
     CDS             complement(876..1637)
                     /locus_tag="VIX62_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEY5861114.1"
                     /translation="MSKEMLNDKTFANVEQYQPVAIPGRPDTVERTGVMTINGTIARA
                     GFLFVLTVICAVFGWQQADQISGVLNAIMLIGLLGLIGLSLLTAFKPQIAPLTGPVYA
                     IVMGFWAGVISYGYEQQFDGIVLQAVFATFSVFAGCLFLYGSRIVKVTKKFIAVVMVA
                     TLGILLMYVAAIVLGLFGVEIGFINDPTPLGIAISVAICIVAALNLFVDFHFIEQGAR
                     AGAPTYMSWYCAFGLLATLIWLYLEILRLLSKIRG"
     gene            complement(1695..1770)
                     /locus_tag="VIX62_12590"
     tRNA            complement(1695..1770)
                     /locus_tag="VIX62_12590"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1735..1737),aa:Ala,seq:tgc)
     gene            1873..2505
                     /locus_tag="VIX62_12595"
     CDS             1873..2505
                     /locus_tag="VIX62_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861115.1"
                     /translation="MRTTRWVGAIVSTMVLLSIALAPSASAKGERGSVTVTVPGSEDV
                     KMRKDDAAEFVYVTGLWEGKWDSPNVDGEMQTVSALGVGYAMVVRFGPECPGDRVHQT
                     IYPYVEGGPQVFTPEGETLCGGEVSAGYYPARTQAFDMLVAAGLPAVAPEPAEEAGAT
                     PTPAAATGAGSGGSSGGWWIALVAIAITLGGYLVVRARRPRTAAEPHPTA"
     gene            complement(2516..3076)
                     /locus_tag="VIX62_12600"
     CDS             complement(2516..3076)
                     /locus_tag="VIX62_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861116.1"
                     /translation="MKKRVVQAGEFEVVDERGAVRLRIGLTNDEDPFFSLLSPDGQVR
                     MRFGLGNDGSAGLAIGDDNGKVRATVGLSSDGSASLAFGDKNGKIRAKFGLAPEASPT
                     MGMQVKDGELSQVYSLAEQGSPTIGLYDNAGKVRVRLGLAAEGSPVMRLLDRDGDLRA
                     VVGLASDGTPFVQFLDEGKEPTWTMR"
     gene            complement(3163..4758)
                     /locus_tag="VIX62_12605"
     CDS             complement(3163..4758)
                     /locus_tag="VIX62_12605"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEY5861117.1"
                     /translation="MAGAAAFFLLKGSGEQLLDNVPASADVVAAVYLDPSAGQKTNLL
                     RLADEIPALGSREQLTSQAQDTIDQLLSQAGLGHEDLEWVGAEVAVAVSLPDDDTEPG
                     VVVLIDADDEAAAAQTLADLRESDAGAAWETRDHDGVEVWAATNDALDSGAYAIVDGV
                     VAASNDQALIDEVIDVTHGNGAAIADDEAFTETTGELPDGKLAMIYVNPGDLVQRVNE
                     LQGLTSDPDLAESLEAAEALTGMAMSLSAESDGMAVDVEVSIDPSKLSDSMRGTLSEE
                     PAEKALLTAVPNDALVVMAQQGLDASIADAVDQIETFSPDVAEMLTDAGVTGEGGVVD
                     SLTGDIALTALPGVDGGTPSGAFLIGTEDEAALSDAANDLVQQFTPDSVITSGNGGKD
                     SSSTVEWRTEDYQGVEVHTLGGSPDESLTEVSYAVIEGAGVIALTPDAMHAVIDAQQG
                     GDGITTSSAYADAMDGMPDGSVSVYVDIDGIADAIRATIPPDQAAEFDRFAGETMDHL
                     DSFVVGSEQSEEHVHVRMFLRVP"
BASE COUNT          814 a         1777 c         1721 g          808 t
ORIGIN      
        1 tccagggcga actcggatac gtgtacaacg cgcacctctc ggcctacggc cagctggggt
       61 acgtccacac cggcgacgtg atcggctacg tcggttccac cggtgatgcc acggcgccgc
      121 acgatcactt cgagtggcac ccgaacgacg ggcccgccgt cgaccccaac ccgtacctgt
      181 cggtttcctg cgacgggtcc tcgccgagct agcggccgtt cgcggagaag tgctggtagt
      241 ccttctcgcc cgaccagtcg ccgccccagc tccagcccac cgcgtggaag gcccaccaca
      301 ccgcatcgtg gcgatggatc atcccccgcc gatgctgtga gcggtccagg aacgggcgac
      361 cgttcgccgg cgagaaccct ccggcgtaaa cgtaggggtt ctccacggtg ttcaggtcga
      421 gcgcgcgtcc gtacgcgtgc tgggaccagg tgcagcagat gccggctcga taccgacagt
      481 tgaacgccga ggtgttgtcc gcggccatcg accggtggtc gtcggcaccg aaatgatcca
      541 ccagtcgcat ctgccggatc gggtagcgca cctcgtacaa ccgtttgaac acccgcacca
      601 tctcgtccgt gtaccggcgg tgcaccacca agctgcctcg gtgccaccgt cgatcgaagc
      661 cccagatctt catgcggatc aatcgcaggt cgcgcagcgg caccgggcat cccgggcgcc
      721 aggagcggcc gaccatcagg tggcgggtgg aggcgtcgag cgggctcacg cgcgcgctga
      781 actccggccg cgcctgcgcc ggggcggcga gggcgagcag gccggtgacg atcagcgcga
      841 gcgcgatgag ccgacgacgc accggtgcct cctcctcagc cacggatctt gctcagcagg
      901 cgcaggatct ccaggtacag ccagatcaag gtcgccagca gtccgaacgc gcagtaccac
      961 gacatgtacg tcggtgcgcc cgctcgagcg ccctgctcga tgaagtggaa atccacgaac
     1021 aggttgagcg ctgccacgat gcagatggcg acgctgatcg cgatacccaa cggcgtcggg
     1081 tcgttgatga acccgatctc cacaccgaac agccccagca cgatcgcggc gacgtacatc
     1141 agcaggatcc cgagggtcgc gaccatcacc accgcgatga acttcttcgt gaccttcacg
     1201 atccgtgagc cgtagaggaa cagacatccg gcgaagaccg agaacgtcgc gaacacggcc
     1261 tgcagcacga tcccgtcgaa ctgctgctcg tagccgtacg agatcacccc ggcccagaag
     1321 cccatcacga tcgcgtagac gggcccggtg agcggcgcga tctgcggctt gaacgcggtg
     1381 agcaggctga gcccgatcag ccccagcagc ccgatgagca tgatcgcgtt caggactcca
     1441 ctgatctggt cggcctgctg ccagccgaac accgcacaga tcacggtgag cacgaacaag
     1501 aaccccgcgc gggcgatcgt gccgttgatc gtcatgacgc cggtgcgttc cacggtgtcg
     1561 ggacgaccgg ggatcgcaac gggctggtac tgctcgacgt tggcgaacgt cttgtcgttc
     1621 agcatctctt tggacatggg ggctcctggt tcgacgggat ctccggggga tggtatctcg
     1681 ccgcgcggtg cgggtggtgg agacgaccgg gatcgaaccg gcgacctccg gcttgcaaag
     1741 ccggcgctct cccaactgag ctacgtcccc gaggcgaccc cagtgtacgc ggcgaccatc
     1801 cccatcccga tggaacctct gcacctcggg cggtgtagac ggcacatgag aacccttcga
     1861 ggaggaaccg ccatgcgaac cacacgctgg gtcggcgcga tcgtgtccac gatggtgctg
     1921 ctgtcgatcg cgctcgcgcc gtcggcttca gccaagggcg aacgcgggag tgtgacggtc
     1981 acggtgccgg gctccgagga cgtgaagatg cgcaaggacg acgcggccga gttcgtctac
     2041 gtcacgggtc tgtgggaggg caagtgggac agccccaacg tcgacggcga gatgcagacg
     2101 gtgtctgccc tcggcgtcgg ctacgccatg gtggtgcggt tcggaccgga gtgccccggc
     2161 gatcgcgtgc accagacgat ctacccgtac gtggagggtg ggccgcaggt gttcacgccg
     2221 gagggcgaga ccctctgcgg cggcgaggtc tccgctggct actaccccgc acgaacccag
     2281 gcgttcgaca tgctcgtggc cgcgggtctg ccggcagtgg ctcccgagcc ggccgaggag
     2341 gccggtgcca caccgacccc ggcagcggcc acaggagccg gatcaggtgg atcctcgggc
     2401 ggctggtgga tcgccctcgt cgcgatcgcg atcaccctgg gtggatacct cgtggttcga
     2461 gcccggaggc cccgcaccgc cgcggagccc catcccaccg cctgagggac ggggcctagc
     2521 gcatcgtcca ggtgggctct ttgccttcgt cgaggaactg cacgaacggc gtcccgtccg
     2581 aggccagacc caccaccgcc cgcagatcgc cgtctcggtc cagcaggcgc atcaccgggg
     2641 agccttcggc cgcaaggccc agtcgcacac ggaccttgcc cgcgttgtcg tagagcccga
     2701 tcgtcggcga accctgttcg gcgaggctgt acacctggga gagttcgccg tccttgacct
     2761 gcatacccat ggtggggctc gcctcgggcg ccaggccgaa cttcgcccgg atcttcccgt
     2821 tcttgtcgcc gaaggccagg ctggccgaac cgtcgctcga caaaccgacg gtggcgcgca
     2881 ccttgccgtt gtcgtcgccg atcgcgagcc cggccgaacc gtcgttgccc agcccgaacc
     2941 gcatgcgcac ctgcccgtcg ggcgagagca acgagaagaa ggggtcctcg tcgttggtga
     3001 ggccgatgcg gagtcgcacc gccccgcgtt cgtcgacgac ctcgaattct ccggcctgga
     3061 cgacgcgctt cttcatgggc tgctcctacg aaggggaagg gtgagcgtgc gcgtatcgtc
     3121 gcccttcgcg gcccgttgtt caaacaggcc ggagtgatgg gctcagggca cgcgcaggaa
     3181 catccgcacg tggacgtgct cctcggactg ctccgagccc accacgaacg agtcgagatg
     3241 gtccatggtc tcgcccgcga accggtcgaa ctcggccgct tgatcgggag ggatcgtcgc
     3301 gcggatcgcg tcggcgatgc cgtcgatgtc cacgtacacg gagacgctgc cgtccggcat
     3361 cccgtccatc gcgtccgcat acgccgagga ggtcgtgatg ccgtcgccgc cctgttgggc
     3421 atcgatcacc gcgtgcatcg cgtcgggcgt gagcgcgatc accccggccc cctcgatcac
     3481 ggcgtagctc acctcggtca gcgactcgtc cggggacccg cccaaggtgt ggacctcgac
     3541 gccctggtag tcctcggtcc gccactccac tgtcgaggac gagtccttcc ctccgttgcc
     3601 ggaggtgatg acgctgtccg gagtgaactg ttggacgagg tcgttcgcgg cgtcactgag
     3661 cgcggcctcg tcctccgtac cgatgaggaa ggcccccgac ggtgtcccgc catctacacc
     3721 cggcaacgcc gtgagcgcga tgtcgccggt caacgagtcg acgacgccgc cctcaccggt
     3781 gaccccggcg tcggtgagca tctcggcgac gtcgggggag aaggtctcga tctgatccac
     3841 cgcgtcagcg atcgatgcgt cgagaccttg ctgggccatg accacgaggg cgtcgttcgg
     3901 cacggccgtg agcaacgcct tctccgccgg ctcctccgat agggtgccgc gcatcgagtc
     3961 gctgagcttg ctcggatcga tcgagacctc gacgtccacc gccatgccgt cggactcggc
     4021 cgacaggctc atcgccatgc ccgtgagggc ctccgcggcc tcgaggctct ccgcgaggtc
     4081 cgggtcggac gtcagtccct ggagctcgtt cacccgctgc accaggtcgc cggggttgac
     4141 gtagatcatc gcgagcttgc cgtcggggag ctcgccggtg gtttcggtga acgcctcgtc
     4201 atcggcgatc gcggcgccgt tgccgtgcgt gacgtcgatc acctcgtcga tcagggcttg
     4261 gtcattggag gcggcgacca ccccgtcgac gatggcatag gcgcccgagt ccagggcatc
     4321 gttcgtggcc gcccagacct cgacgccgtc gtgatccctg gtctcccagg ccgcccccgc
     4381 gtcgctctca cgcaggtccg cgagggtctg cgccgcagcc gcttcgtcgt cagcgtcgat
     4441 cagcactacg acgcccggct cggtgtcgtc gtcgggcagc gacaccgcca cggccacctc
     4501 cgcacccacc cactcgaggt cctcgtggcc caggccggcc tgggacaaca gctgatcgat
     4561 cgtgtcttgg gcctgggacg tgagctgctc acgcgaaccc agggccggga tctcgtcggc
     4621 caggcgcagc aggttcgtct tctggccggc ggagggatcc aggtacacgg ccgccacgac
     4681 gtcggcggac gcggggacgt tgtccaggag ttgctccccc gagcccttca gcaggaagaa
     4741 cgcggcggct cccgccacga ccgcgatccc cccacggccg ccaccgcgat cagtgccacc
     4801 ttggtggcac gtccgcccgt cggtggtggc gggggcgtct gatccccgcc gtcgggcatc
     4861 ggcggcacgt cggacggggg tggcggcgga acgcgatcca cggtgggcgg cctttcgagt
     4921 ggtccggggg gctcggtcgg cgttgagcct agcggcaacg acaggccggc ccaaggatgc
     4981 ggcgggatca gggtcgaagc gtctcaacgt gcaacgtgca cgcctccagg cggtcggttc
     5041 ggggcgtgac cccgattccc gctccggcgg gcaccgccat ggtgccgtcg ggtgccatca
     5101 cgaacggctc ggtgagatcg
//
LOCUS       DATDBZ010000546         3103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_168030, whole genome shotgun sequence.
ACCESSION   DATDBZ010000546 DATDBZ010000000
VERSION     DATDBZ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168030"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VIX62_12610"
     CDS             <1..205
                     /locus_tag="VIX62_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861118.1"
                     /translation="DDTLTVEIAPGTQVRILRAGVGRRLTADDEDYDDEDYDDEDYDE
                     DEDGDEGGEHDTSDEQQGPIQQL"
     gene            202..978
                     /locus_tag="VIX62_12615"
     CDS             202..978
                     /locus_tag="VIX62_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5861119.1"
                     /translation="MSDADLPREPVRRVDEPAPFVPHQALPEHPAPTPGSDAPPSVSL
                     EMIQDHEPLAAFIASADRVMEAMGFTEHGFRHANLVSSISFQVLHRLGYGEREATLAS
                     VAGYLHDVGNALARDAHGQTGAVIVYQALHDTVNPADLMPIVAAIANHEETEGTAVSP
                     VSAAVILADKSDVHRSRVRKSGHIDHDIHDRVNYAVEQSFLRVDSDARTVSLELTIDT
                     QISQVMEYFEIFLGRMQLCRRAAEMLDARFRLVVNGSELA"
     gene            975..>3103
                     /gene="secD"
                     /locus_tag="VIX62_12620"
     CDS             975..>3103
                     /gene="secD"
                     /locus_tag="VIX62_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY5861120.1"
                     /translation="MKRTRGLWLSIAMVALLVVGSLIGFATGALQPTLGLDLEGGVSV
                     ILTAPEGTPRDVMDQALTNIRNRVDAFGVGEPDIVLSGNTIEVQIPGLTNGTIEERQK
                     EQYCLIDDGGVNYGCGADQAEAQAALDDLQVTSQASEACVEDADGTQLECFTSEQEAT
                     AFQSSITVSPKTAASPSASPSGSATPSASPSSEPDTGGFCLTDPTGKQYGCSTTKKEA
                     QKAADGLTVEVTERSFCVTGGATATEEEASPTPSATPDPSTSASPSPSASASPSASPS
                     ASGVSGLDLQGTETLPCELETKADAQDALDAIAVSKQTVQYCVISSAAQNLGCFIDRD
                     AAEQERRATGQQRLLSVIGQTARLEQRPVLAIVPPSDPTYTSLAVTCPTAEEADTDAC
                     SFDTLDQQEVVYMDAQKNKFRLGPTVISGSDITNATAVFGGSGVTQWQVSFELGGDAV
                     DAFAEATTTAVSQPPPQNSIAIVVDREVISYPTVNDPITTGSGVISGGFDETEAKDLA
                     TVLNAGALPVELTRQSVQTVSPTLGEESLRQGIVAGIAGLILLFLYLLFYYRLLGIVA
                     WFGMSAWAILALAIVSIAGAQVGYALTLAGVAGLVISLGVTADSYIVFFERLKDEVRS
                     GKSARSAVQPAFKRSYKTIVAADFVTMIAAVVLYVTAVSSVRGFALTLGVATLLDLFV
                     VYFFKRPTVFLIARNERLVNMHGFGLR"
BASE COUNT          521 a         1054 c         1058 g          470 t
ORIGIN      
        1 cgatgacacc ctgaccgtcg agatcgctcc cggcacgcag gtccggatcc tgcgcgcggg
       61 cgtgggtcgc cggctcacgg ccgacgacga ggactacgac gacgaggact acgacgacga
      121 ggactacgac gaggatgagg acggcgacga gggcggcgag cacgacacgt ccgacgagca
      181 gcagggcccc atccagcagc tgtgagtgac gccgaccttc cccgcgagcc cgttcgccgg
      241 gtcgatgaac ccgcgccctt cgtgccgcat caagcgttgc ccgaacaccc ggcgccgaca
      301 cccggctcgg acgccccgcc gtcggtctcg ctggagatga tccaggacca cgagccgctc
      361 gcggccttca tcgcctcggc cgaccgcgtg atggaggcga tggggttcac cgagcacggg
      421 ttccgccacg cgaacctggt gtccagcatc tcgttccagg tcctgcaccg gctgggctac
      481 ggcgagcgtg aggcgacgct ggcgagcgtg gccgggtacc tgcacgacgt cggcaacgcc
      541 ctggcgcggg acgcccacgg ccagaccggt gcggtgatcg tctatcaagc gctgcacgac
      601 accgtgaatc cggcagacct catgccgatc gtggccgcga tcgcgaacca cgaagagacc
      661 gaggggaccg cggtctcccc ggtgtcggcc gcggtgatcc tggccgacaa gagcgacgtg
      721 catcgaagcc gcgtgcgcaa gagcgggcac atcgatcacg acatccacga ccgcgtgaac
      781 tatgccgtgg agcagtcgtt cctgcgggta gactcggacg ctcgaacggt cagcctggag
      841 ctgaccatcg acacccagat cagccaagtg atggagtact tcgagatctt cctgggcagg
      901 atgcagctgt gccgtcgcgc ggctgagatg ctcgacgccc ggttccgact ggtcgtgaac
      961 ggctcggagc tggcgtgaag cgaacccgcg gcttgtggct gtcgatcgcc atggtggcgc
     1021 tgctcgtcgt ggggtccctg atcggtttcg ccaccggtgc cctgcagccc accctgggcc
     1081 tggacctcga gggcggcgtc tcggtgatcc tcaccgcgcc ggaaggcacg ccccgcgacg
     1141 tcatggacca ggcgctcacg aacatccgga accgcgtcga cgcgttcggg gtgggggagc
     1201 ccgacatcgt gctgtcgggc aacacgatcg aggtgcagat ccccggcctg acgaacggca
     1261 cgatcgagga gcgtcagaag gagcagtact gcctgatcga cgacggcggc gtgaactatg
     1321 gctgcggcgc cgaccaggcc gaagcgcagg cggcgctcga tgacctgcag gtgaccagcc
     1381 aggcctcgga agcctgcgtc gaggacgcgg atggcaccca gctcgagtgc ttcaccagcg
     1441 aacaggaagc caccgccttc cagtcgtcca tcaccgtgtc gcccaagacg gccgcaagtc
     1501 cctcggcctc gcccagcggt tcggcaacgc cgtcggcgag cccgtcgtcc gaacccgaca
     1561 cgggtgggtt ctgcctcacc gaccccacgg gcaagcagta cggatgttcc accaccaaga
     1621 aggaagcgca gaaggcggcc gacggcctga cggtcgaggt gacggaacgc agtttctgcg
     1681 tcaccggcgg cgccaccgcc accgaagagg aggcctcgcc taccccatcg gccacacccg
     1741 acccttcgac gtccgcgtca ccgtcgccca gtgcgtcggc ctcacccagt gccagcccgt
     1801 ccgcgagcgg ggtgtccggc ctcgatctgc aagggacgga gacgctcccg tgcgagctcg
     1861 agacgaaagc cgatgcgcag gatgcgctcg atgcgatcgc ggtgtccaag cagaccgtgc
     1921 agtactgcgt gatcagctcg gccgcccaga acctgggctg cttcatcgac cgggacgccg
     1981 ccgagcagga gcgtcgcgcg acaggccagc agcgtctgtt gagcgtgatc ggacagaccg
     2041 ctcggctgga gcagcgcccg gtcctcgcca tcgtcccgcc gtctgacccc acgtacacgt
     2101 cgttggcggt gacctgtccc accgctgagg aggcggacac cgacgcctgc tcgttcgaca
     2161 cgctggacca gcaagaggtc gtctacatgg acgcccagaa gaacaagttc cggctgggac
     2221 cgaccgtgat cagcggcagc gacatcacga acgccaccgc cgtgttcggc gggagcggcg
     2281 tcacccagtg gcaggtctcg ttcgaactcg ggggcgacgc ggtcgatgcg ttcgccgagg
     2341 ccaccacgac ggccgtgagc cagccgccgc cgcagaactc gatcgcgatc gtggtcgacc
     2401 gcgaagtgat ctcctatccc acggtcaacg accccatcac gaccggcagc ggcgtgatca
     2461 gtggcggctt cgacgagacc gaggccaagg acctggcgac cgtgctgaac gccggtgcac
     2521 tgcctgtgga actgacgcgg cagtcggtgc agaccgtgag ccccacgctg ggtgaggagt
     2581 cgttgcgaca ggggatcgtg gccgggatcg ccggcttgat cctgctgttc ctgtacctgc
     2641 tcttctatta ccggctgctg ggcatcgtgg cgtggttcgg catgtcggca tgggcgatcc
     2701 tggcgctggc catcgtctcg atcgccgggg cgcaggtggg ctacgcgctc accctggccg
     2761 gcgtcgcggg cctcgtgatc tccctgggcg tgaccgccga ctcctacatc gtgttcttcg
     2821 agcggctgaa ggacgaggtg cgctcgggca agtcggctcg ttcggccgtg cagccggcgt
     2881 tcaagcggtc gtacaagacg atcgtggcgg ccgacttcgt gacgatgatc gcggcggtgg
     2941 tgctctacgt caccgcggtg agctccgtgc gcgggttcgc gctcacgctg ggcgtggcga
     3001 cgctgctcga cctgttcgtc gtgtacttct tcaagcgacc cacggtgttc ctgatcgcgc
     3061 ggaacgagcg gctcgtgaac atgcacgggt tcggacttcg cag
//
LOCUS       DATDBZ010000547         4764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_3045067, whole genome shotgun sequence.
ACCESSION   DATDBZ010000547 DATDBZ010000000
VERSION     DATDBZ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3045067"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /gene="modA"
                     /locus_tag="VIX62_12625"
     CDS             <1..358
                     /gene="modA"
                     /locus_tag="VIX62_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5861121.1"
                     /translation="AEGVPVGDYAREALDNAGILDEALANVVSNEEDNASVVAKVTAN
                     EADAAIVYTSDVSDAAGNDVNAVTIPEDVNVIAMYPVAVVEGALNADLAADFVAYVVS
                     SDGQATLKEYGFEPTA"
     gene            358..1140
                     /gene="modB"
                     /locus_tag="VIX62_12630"
     CDS             358..1140
                     /gene="modB"
                     /locus_tag="VIX62_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY5861122.1"
                     /translation="MHRRLPLGITILAVIGAAFVVLPVLALAIRAPWGDLSTSLSGVG
                     ATTALRLSIEVSLAATALSLLFGVPLAWLLARGTFPGRSVLRAMVVLPIVLPPVVGGI
                     GLLNALGRSGVAGQWLYEGLGIQLTFTIWGAIIAATFVSMPLVVLATEAGLHSLDQRY
                     EMAATTLGASQGVAMRRVVLPMLMPSIVAGAVLCWARALGEFGATITFAGNLEGRTQT
                     LPLAVYQARQTDPGGAIFLSLILVVISIVVLVAMRDHLMKAR"
     gene            1142..2269
                     /locus_tag="VIX62_12635"
     CDS             1142..2269
                     /locus_tag="VIX62_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011210864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5861123.1"
                     /translation="MGLRADLAITRGAFDVRLELEAADGETLALLGPNGAGKTTVVEA
                     LAGLAEAAEGATIELDGERIDRLPPERRRVGVCFQDDLLFPHLSVLENVAFGLRARGT
                     AKPVARTRAGELLRRFASGVRPAAKPRELSGGERRRVSLARALATDPRLLLLDEPLAG
                     VDVTARGQIRSLLREVADGFDGVVVLVAHDPLDALTLADRVAIMEGGRLTQEGTPDEI
                     RSAPRSPYAADLVGVNLFTGALEPLEDGAATLQTSLGTITVAPEGAPESGTAAFATLR
                     PADVSLHLERPEGSARNVFEGTVDEVAIDAERARVRLDTHPPLIAEVTAGSVARLGLA
                     PGTHAWASFKAVEVSLHLVDQAEDRALQPATESTATGTLSE"
     gene            2266..2961
                     /gene="lepB"
                     /locus_tag="VIX62_12640"
     CDS             2266..2961
                     /gene="lepB"
                     /locus_tag="VIX62_12640"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY5861124.1"
                     /translation="MKTTERESLEAPEPGDDAPDEEHKHKNPVVQFLGELPGLIIMAF
                     VLALLIKTFLVQAFFIPSGSMEPTLMPGDRVLVLKVPYYFHDPRRGDIIVFEDPDPTD
                     QPDRGAVGGFFHWMFEGLGVQRPDNEDFIKRVIGVPGDTVFAKDGQVFVNDQPIDEPY
                     LTQKTADFAPVHVPDDMLFVLGDNRGNSQDSRFGLGIATKGSKGVGFIPIDKVIGKAE
                     IIIWPPSRLGLLH"
     gene            complement(2973..>4764)
                     /locus_tag="VIX62_12645"
     CDS             complement(2973..>4764)
                     /locus_tag="VIX62_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY5861125.1"
                     /translation="VQLAQDARRFTLTRSELRSGIDRILTYVTFAIIPTAVLLFISQL
                     RASDDWREAVSGAVAGTVAMVPEGLVLLTSLAFAVAVRRLARQRVLVQELPAVEGLAR
                     VDMLCIDKTGTLTQGKLAVEDVDLLTGQDDDAATDALAAIAAADSAPNATMLAIRDRF
                     DQPPDWKADAAVPFSSARKWSGASFDGHGAWLLGAPDVLMGAIGGHDEVHERVGALSD
                     EGKRVVLLASADRLDGDELPQALEPVALVILGDLPRPDAADTLRYFREQDVTVKVISG
                     DDPRTVGAIARQLGLPDADEPIDARTLSEDLDELAGTLESHGVFGRVTPQQKRAMVQA
                     LQAHGHTVAMTGDGVNDALALKDADIGVAMGSGSDATRAVAQLVLLDSNFDALPPVVA
                     EGRRVLGNIERTSGLYLNKTVYAMLISLATGVAGFVFPFLPRHLTLIGALTIGIPSFF
                     LALAPNTERFRPGFVERVLRFAIPTGTLAAIATLLAYRLAANEPGVTLLEAQTTAVMV
                     LTWIGFLVLSILSAPLTRSRLALIWTMAGLFVIALIWPPSATFFALDDPPAIVWLAGF
                     GIAAIVWSFARLFVPGEQPVGPRNRAVPRK"
BASE COUNT          738 a         1650 c         1651 g          725 t
ORIGIN      
        1 cgccgaagga gtgcccgtgg gcgactatgc gcgagaggct ctcgacaacg cagggatcct
       61 cgacgaggcg ctcgcgaacg tcgtgtccaa tgaagaggac aacgcgagcg tggtcgcgaa
      121 ggtcaccgcc aacgaagccg acgccgccat cgtttacacc tccgacgttt ccgatgccgc
      181 cggcaacgac gtcaacgcgg tcacgatccc cgaggacgtc aacgtcatcg ccatgtaccc
      241 cgtcgccgtg gtcgagggtg cgctgaatgc cgatctggcc gcggacttcg tcgcctacgt
      301 ggtgagttcc gacggccaag cgacgttgaa ggagtacggc ttcgagccga ccgcctgatg
      361 caccggcgcc ttcccctggg catcacgatc ctcgccgtca tcggcgcggc gttcgtcgtg
      421 ctcccggtgc tggcactggc gatccgggcg ccgtggggcg acctctcgac gtcgctctcg
      481 ggggtcggcg ccaccacggc gctgcgcctg tcgatcgagg tctcgctcgc cgcgaccgcc
      541 ctgtcgctgc tgttcggtgt tccgctcgcc tggctcctgg cgcgcgggac gttcccagga
      601 cggtcggtgc tacgcgcgat ggtcgtgctg ccgatcgtgt tgccgcccgt ggtcggcggg
      661 atcggactcc tcaatgccct cggtcgcagc ggggtcgccg ggcagtggct ttacgagggc
      721 ctcgggatcc agctcacgtt cacgatctgg ggggcgatca tcgcggcgac cttcgtgtcg
      781 atgccgctcg ttgtgctagc caccgaagcg ggactccact cgctggatca gcgctacgag
      841 atggccgcga ccaccctcgg ggcctcgcag ggtgtggcga tgcgccgggt ggtgctcccc
      901 atgctcatgc cctcgatcgt ggccggcgcg gtgttgtgct gggcgcgtgc gctcggtgag
      961 ttcggagcca cgatcacgtt cgcgggcaac ctggaaggcc ggacccagac gctgccgctc
     1021 gcggtgtacc aggcgcgtca gacggacccg ggcggcgcca tcttcctgtc gttgatccta
     1081 gtggtgatct cgatcgtggt gctggtcgcg atgcgcgatc acctgatgaa ggcgcgctga
     1141 cgtgggactg cgagccgacc tcgccatcac ccgcggggcc ttcgacgtac gcctcgagct
     1201 ggaagcggcc gacggcgaga ccctcgcgct cctcggaccc aacggcgccg gcaagaccac
     1261 cgtcgtcgaa gcgctcgcgg gcctcgccga ggccgccgaa ggtgccacca tcgagctcga
     1321 cggtgaacgc atcgatcggc tcccgcccga gcgacgccgg gtcggcgtgt gcttccagga
     1381 cgacctgttg ttcccccacc tgtcggtcct ggagaacgtg gcgttcggcc ttcgggcgcg
     1441 cggcacggcc aagccagtcg cacgcacccg cgccggcgaa ctcctcaggc ggttcgcgtc
     1501 cggggtgcgg ccggccgcga agccgcggga gctctcgggt ggggaacggc ggcgcgtctc
     1561 gttggctcgc gccctcgcca ccgacccccg gctgctgctg ctggacgaac ccctcgccgg
     1621 cgtggacgtc accgcccgcg ggcagatccg atcgctgttg cgcgaggtcg ccgacgggtt
     1681 cgacggcgtc gtcgtgctcg tggcgcatga cccgctcgac gccctcaccc tggcggatcg
     1741 tgtcgcgatc atggagggcg gccggctcac ccaggagggc acgcccgacg agatcaggag
     1801 cgcgccacga tcaccgtacg ccgccgacct ggtgggcgtg aacctgttca ccggggcgct
     1861 ggaaccgctc gaggacggcg ctgccacgct gcagacgtcc ctcggcacga tcaccgtcgc
     1921 ccccgaaggc gcgccggagt ccgggaccgc ggcgttcgcc accctgcgtc cggccgacgt
     1981 ctcgctccac ctggaacgtc ccgaaggatc ggctcgaaac gtgttcgagg gcaccgtgga
     2041 cgaggtcgcc atcgacgccg agcgggcccg ggttcggctg gacacgcacc cgccgttgat
     2101 cgccgaggtc accgcggggt cggtcgcgcg gctgggcctg gcgccgggga cccacgcctg
     2161 ggccagcttc aaggccgtgg aggtctcatt gcatctcgtc gaccaggccg aggaccgggc
     2221 gttgcaaccg gcaacggagt cgaccgcaac cggtaccctg agcgagtgaa aacgaccgag
     2281 cgggagtcgc tcgaggctcc cgagcccggc gacgacgccc ccgacgaaga gcacaagcac
     2341 aagaacccgg tggtgcagtt cctgggagag ctgcccggtc tcatcatcat ggccttcgtc
     2401 ctggccctgt tgatcaagac cttcctggtg caggcgttct tcatcccgtc gggttcgatg
     2461 gaacccaccc tgatgccggg cgaccgggtc ctggtgctga aggtccccta ctacttccac
     2521 gacccgcggc gcggcgacat catcgtgttc gaagatcccg accccaccga ccagcccgac
     2581 cgcggcgccg tcggcgggtt cttccactgg atgttcgagg gtcttggggt ccagcggccc
     2641 gacaacgagg acttcatcaa gcgggtgatc ggggtgccgg gtgacaccgt gttcgccaag
     2701 gacgggcagg tgttcgtgaa cgatcaaccg atcgatgagc cgtacctcac acagaagacg
     2761 gccgatttcg cgccggtgca cgtgcccgac gacatgctct tcgtgctggg tgacaaccgc
     2821 gggaactctc aggactcacg gttcggtctg ggcatcgcca cgaaggggtc gaaaggcgtg
     2881 gggttcatcc ccatcgacaa ggtgatcggc aaggccgaga tcatcatctg gccgccttcg
     2941 aggctgggct tacttcacta gtccagctca gactacttcc gcgggacggc ccggttcctc
     3001 ggcccaacgg gctgctcccc cggcacgaac aaccgcgcga acgaccacac gatcgctgcg
     3061 atgccgaacc cggcgagcca cacgatcgcc ggcgggtcat cgagcgcgaa gaaggtggcg
     3121 ctgggcggcc atatcagcgc gatcacgaac aagcccgcca tcgtccagat caacgcgagc
     3181 cgtgatcgcg tgagcggcgc cgacaggatc gacaacacca ggaagccgat ccaggtcagc
     3241 accatcaccg cggtggtctg cgcttcgagc agggtgaccc ccggttcgtt cgccgccaac
     3301 cggtacgcga gcagggtggc gatcgccgcg agcgtgccgg tggggatcgc gaaccggagc
     3361 acgcgctcca cgaaccccgg gcggaaccgt tcggtgttgg gcgcgagcgc caggaagaac
     3421 gacgggattc cgatcgtcag cgcaccgatc aacgtcaggt gccgcggcag gaacgggaac
     3481 acgaatccgg ccacgcccgt cgccagcgag atcaacatcg cgtacacggt cttgttcaga
     3541 tacaggccgc tcgtgcgctc gatgttgccc aggacccggc gaccctcggc caccaccgga
     3601 ggcagcgcgt cgaagttgga atccaggagc acgagctggg ccaccgcgcg ggtggcatcg
     3661 ctcccagatc ccatcgcgac gccgatgtcg gcgtccttca gcgcgagcgc gtcgttcacg
     3721 ccgtcgccgg tcatcgcgac ggtgtggccg tgcgcctgca atgcctgcac catggcccgc
     3781 ttctgttgcg gcgtgacgcg gccgaacacc ccgtgggatt cgagcgtgcc cgcgagttcg
     3841 tcgagatcct cggaaagcgt gcgggcgtcg atcggttcgt cggcgtcggg cagacccagc
     3901 tgccgggcga tcgctcccac ggtgcggggg tcgtcgcccg agatcacctt gacggtgacg
     3961 tcctgctcgc ggaagtaccg cagcgtgtcg gcggcatcgg gccgcgggag atcgcccagg
     4021 atcacgagcg cgacgggctc gagggcctgc ggcagctcgt cgccgtcgag ccggtcggcc
     4081 gaggccagga gcacgacgcg cttgccctcg tcggacaagg cgccgacgcg ttcatgcacc
     4141 tcgtcgtgcc caccgatcgc acccatcagg acgtcgggag ctcccagcag ccaagcccca
     4201 tgaccgtcga acgaggcgcc gctccacttg cgggcgctgg agaacggcac cgcggcgtcg
     4261 gccttccagt caggaggctg gtcgaaccgg tcgcggatcg cgagcatcgt ggcgttgggc
     4321 gccgagtcgg cggccgcgat ggccgcgagc gcatcggtgg cggcgtcgtc gtcctgcccc
     4381 gtcaacaggt cgacatcctc gaccgcgagc ttgccctggg taagcgtgcc ggtcttgtcg
     4441 atgcacagca tgtcgacgcg cgcgagccct tcgacggcgg ggagctcctg caccaggacg
     4501 cgttggcgcg cgagccgtcg cacggccacg gcgaaggcca gcgaggtcag cagcaccagc
     4561 ccttccggca ccatcgcgac ggtgccggcg acggcgcccg agaccgcttc acgccaatcg
     4621 tcggaggcgc ggagctggga gatgaacagc agcaccgcgg tggggatgat cgcgaacgtg
     4681 acgtaggtga ggatgcggtc gatgcccgag cgcagttccg agcgggtcag ggtgaaccgg
     4741 cgcgcatcct gcgccagctg cacc
//
LOCUS       DATDBZ010000548         5333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_584022, whole genome shotgun sequence.
ACCESSION   DATDBZ010000548 DATDBZ010000000
VERSION     DATDBZ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584022"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VIX62_12650"
     CDS             <1..858
                     /locus_tag="VIX62_12650"
                     /inference="COORDINATES: protein motif:HMM:NF039601.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5940 domain-containing protein"
                     /protein_id="HEY5861126.1"
                     /translation="KASGVHALQHLLKQGDVDPATITHAIGCGEEAVGDRYQRGGGNV
                     AKAIAEHCGLPNASGIDVKSFCAAPVHALVVAAALIEAGIEERVVVVAGGSMGKLGMK
                     FEGALSKGFPIIEDVLAGMAVLLEPADGSNGPIVRTDAVGRMPVDAGSAPQAQLEALV
                     GSPLDAIGIGIGDIDTYATEIHNPEITEPQGGGDVADRNYKMLAGLGVVRGELERGDI
                     AGFSRAHGMPGFSPTQGHIASAVPWIPHALTAMRAGELQRTMLIAKGSLFLGRLTRLW
                     DGVSVTLEA"
     gene            894..1253
                     /locus_tag="VIX62_12655"
     CDS             894..1253
                     /locus_tag="VIX62_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5861127.1"
                     /translation="MIEATVETFDDLSREGEVLVDIWGPDCQPCLALMPAVEALAETY
                     GDKVRFLKVNAPDNRKICRDLRVAGLPAYLTLRDGVEVGRLTSNATTPEQIEELIVGL
                     MNGAPAVGPPIPEHLRG"
     gene            1261..1719
                     /gene="grdA"
                     /locus_tag="VIX62_12660"
     CDS             1261..1719
                     /gene="grdA"
                     /locus_tag="VIX62_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_082141801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1387..1389,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HEY5861128.1"
                     /translation="MELRGKKVIALGERDSVPGEAIAEVARSAGAEVIEIRTECFVUT
                     ASGAMDPDNQALIKRIIDEIGDTDIVVMLGAADAEAVEVAAETVRDGDPAWVGPLAGV
                     QLGLPVLHVFEDEVKEQADDGVYETQIGFFEMTLDVDGVKDAMKRARGET"
     gene            1716..2585
                     /locus_tag="VIX62_12665"
     CDS             1716..2585
                     /locus_tag="VIX62_12665"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY5861129.1"
                     /translation="MTYPTEPVFGREVFRELARPLQHPEALCWDPLTERIYCGGEGGQ
                     IYAVTLDGSITEVANTGGSVLGMAVDAYGRVYACDWHREEVVRVDAATGTVETYTKGA
                     PDLAIDDPNSPVFADDGTMYVTGSENPAIFRVAPGGETTVWTTAVGGYPNGITLTPDG
                     TAVVVAQSHPDDADGGRVWRIPIQPDGSAGEAVVMAELFHTVPDGVAFDTEGRCYVAH
                     YTPDRIDRILPDGTVETVVEDWEAIHLNAPTNIAFCGPNLEFVAAACVGEEFLAIADL
                     GVQGSPLRRPLVP"
     gene            complement(2591..4000)
                     /locus_tag="VIX62_12670"
     CDS             complement(2591..4000)
                     /locus_tag="VIX62_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5861130.1"
                     /translation="MSETNVAPPDVPVVRTELPGPQSQALFDRLDPVHMGTLDHREVP
                     FVEARKVDWMIEDVDGNTFADHCSAWGSTPLGATPEAVQRAVTEAQLRYGMEISNYVA
                     TEPVVALAERLVEIAPPGLTRISMGVSGTLVVEAGVKLAREATGRPMILSFYGQYHGE
                     STYLTAGMSTDLSEVPTQHAQYVAGLVFAPYPNRFRAPFHSGPGPFDDQWYLDYLEEW
                     VLVHQVEPTQIAGVLIEPVLGEGGILIPSQGFWDRLTDMCKRWGWKLILDEVQTCMGR
                     CGTMFAAERFGLEPDILLLGKGFAGGGQPIAAVLGTEDVMAGSSLHLGGTFAWTPAAA
                     AGALANIDAILDSGALDNAMRLEAIALEEFAPAVERHAQVGEVRAAGAFVGIEFVQDA
                     ETLRPAPAFHREVHLAAVRRGILGITQWGKWIYRLQPAIDMPEDLFRWSCRQIVDAID
                     EVAADPPAESASLLDRDGP"
     gene            complement(3997..4863)
                     /locus_tag="VIX62_12675"
     CDS             complement(3997..4863)
                     /locus_tag="VIX62_12675"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5861131.1"
                     /translation="MTIGTNDQEGANATMRFEGRGVIVTGGSRGIGRGIVDAFLDEGA
                     RVLATDVLGEELAQMRDAHPHVDRLAVHTADLADADQVRTIVPAAANLLGGVDVLVNN
                     AGLQPDQHALDVSTEHFDATFAVNVRAPMMLMQDVVRLWIERDTPGAIVNIASANAFQ
                     NESPESIYNASKAALVALTKAYAHEFGHHRIRANVVAPGETITPEAEQDMAQDPRERE
                     LVQRYLTKIPLQYAGTPRDQAMAVLFLASDDARFISAQTLIVDGGELGGGSWYDDADA
                     PPPPPEDRPVTG"
     gene            complement(4860..>5333)
                     /locus_tag="VIX62_12680"
     CDS             complement(4860..>5333)
                     /locus_tag="VIX62_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5861132.1"
                     /translation="PLPDFDLQGYRDLRRQVPAVPVIAAGDAFWDPRLMAEVLRDPPW
                     DAMRFDVSFAGGVTAARSLMAVAAEAGIDVELISYGHTVIQAANLHVALAFERTTYFE
                     QAVPVEPWEHAVRNPIHTGPDGRAHVPHGTGLGIELDQDMVEAATIAQVAVGDLR"
BASE COUNT          840 a         1784 c         1898 g          811 t
ORIGIN      
        1 aaggcgagcg gcgtccacgc gctgcagcat ctgctgaagc agggcgacgt cgatccggcg
       61 acgatcaccc atgcgatcgg atgcggcgaa gaggcggtgg gcgatcgcta ccagcgcggc
      121 ggtggcaacg tcgcgaaggc gatcgccgag cactgcggtc tgccgaacgc cagcggcatc
      181 gacgtgaagt ccttctgtgc ggcccccgtg cacgccctcg tggtggcggc cgccctgatc
      241 gaggccggca tcgaggaacg cgtcgtcgtg gtggccggtg gatcgatggg caagctcggg
      301 atgaagttcg agggtgccct gtcgaagggc ttccccatca tcgaggacgt gctggccggc
      361 atggcggtgt tgctcgaacc cgccgacggg tcgaacggtc cgatcgtgcg gaccgacgcg
      421 gtggggcgta tgccggtcga tgccgggtcg gcaccgcaag cgcagctgga agccctggtg
      481 ggttcgccgc tcgatgcgat cggcatcggg atcggcgaca tcgacacgta cgccaccgag
      541 atccacaacc cggagatcac cgaaccgcag ggcggtggcg acgtcgccga ccgcaactac
      601 aagatgctcg ccggcctcgg ggtggtgcgc ggcgaactgg agcgcggcga catcgccggg
      661 ttctcacgcg ctcacggcat gccggggttc tcgccgaccc agggccatat cgccagcgcg
      721 gtgccgtgga tcccgcacgc cctcacggcg atgcgcgccg gcgagctgca acgaacgatg
      781 ttgatcgcga agggctcgct gttcctcggc cgcctcacgc ggctgtggga cggcgtgtcc
      841 gtgaccttgg aagcgtgacc gaacgcatgc caaagacata tggaggagga ccgatgatcg
      901 aggccaccgt ggagacgttc gacgacctca gccgcgaggg cgaggtgctc gtcgacatct
      961 ggggaccgga ctgccagccg tgcctggcgt tgatgccggc ggtggaggca ttggccgaga
     1021 cttacggcga caaggtgcgg ttcctgaagg tcaacgcgcc cgacaaccgc aagatctgtc
     1081 gcgaccttcg ggtggccggc ctgccggcct atctgacgct gcgcgacggt gtggaggtgg
     1141 ggcggctcac gagcaacgcg acgacgcccg aacagatcga agagctgatc gtcggcttga
     1201 tgaacggcgc gcccgcggtg ggcccgccga tcccggaaca cctgaggggg tgaggaccac
     1261 atggagctca gaggcaagaa ggtgatcgcg ctcggcgaac gcgacagcgt gccgggcgaa
     1321 gcgatcgcgg aggtggcgcg cagcgccggc gccgaagtga tcgagatcag gacggagtgc
     1381 ttcgtctgaa cggcgtcggg cgccatggac cccgacaacc aggcattgat caagcggatc
     1441 atcgatgaga tcggcgacac ggacatcgtg gtgatgctcg gcgcggccga cgcggaagcc
     1501 gtcgaggtgg ccgctgagac cgtccgcgac ggtgatccgg cctgggtggg tccattggcg
     1561 ggggttcagt tgggactccc cgtactgcac gtgttcgaag acgaggtgaa ggaacaggcc
     1621 gacgacggcg tgtacgagac gcagatcggc ttcttcgaga tgaccttgga cgtggacggc
     1681 gtgaaggatg cgatgaagcg cgccaggggc gagacatgac ctaccccacc gagcccgtct
     1741 tcggtaggga ggtattccgc gaactcgcgc ggccgctcca gcatcccgag gcgctctgct
     1801 gggatcccct caccgagcgc atctactgcg gcggcgaggg cgggcagatc tatgccgtga
     1861 ccctggacgg atcgatcacc gaggtcgcga acaccggcgg cagcgtgctc gggatggcgg
     1921 tcgatgccta tggccgcgtc tacgcctgcg actggcaccg cgaggaggtg gtgcgcgtcg
     1981 acgccgccac cggcaccgtc gagacctaca ccaagggggc gcccgacctg gcgatcgacg
     2041 acccgaactc gccggtgttc gccgacgacg gcacgatgta cgtcaccggc tcggagaacc
     2101 cggcgatctt ccgtgtggcg cccggcggcg agaccaccgt ctggacgacc gcggtgggtg
     2161 gctatcccaa cggcatcacg ctcacgcccg acgggaccgc cgtggtcgtg gcgcaatccc
     2221 accctgacga cgccgacggc ggccgcgtgt ggcgcatccc gatccaaccc gacggctcgg
     2281 cgggggaggc ggtcgtcatg gccgagctgt tccacaccgt gcccgacggc gtggcgttcg
     2341 acaccgaggg acgctgctac gtggcgcact acacgccgga tcggatcgat cgcatccttc
     2401 ccgacggcac cgtcgagacc gtggtggagg actgggaggc gatccacctc aacgctccga
     2461 cgaacatcgc cttctgtggc ccgaacctgg agttcgtggc cgcggcatgc gtgggggagg
     2521 agttcctggc gatcgccgac ctcggcgtgc aggggagccc gctccgccgg ccgctggtcc
     2581 cgtaggcgcg tcaggggccg tcgcggtcga gcagcgatgc cgactcggcc ggtgggtcgg
     2641 cggcgacctc gtcgatggca tcgacgatct gccggcatga ccaccggaac aggtcctcgg
     2701 gcatgtcgat cgccggttgc aggcgataaa tccacttgcc ccactgggtg atgcccagga
     2761 tgccccggcg cacggcggcg agatgcacct cgcggtggaa ggcgggcgcc ggcctcagcg
     2821 tctcggcatc ctgcacgaac tcgatcccca cgaacgcgcc ggcggcgcgg acctcgccca
     2881 cctgtgcgtg acgctcgacg gcgggcgcga actcttccag ggcgatggcc tcgaggcgca
     2941 tggcgttgtc gagcgcccca gagtccagga tcgcgtcgat gttggcgagc gcacccgccg
     3001 ccgcggccgg cgtccacgcg aacgtcccgc ccaggtgcag cgaggagccc gccatcacgt
     3061 cctcggtgcc gagcacggcg gcgatcggtt ggccgccgcc ggcgaacccc ttgcccaaca
     3121 gcaggatgtc gggctcgagc ccgaaccgct cggcggcgaa catcgtcccg caccggccca
     3181 tgcacgtctg gacctcgtcc aggatcagct tccaacccca gcgcttgcac atgtcggtga
     3241 gccggtccca gaacccctgc gacgggatca ggatgccgcc ttcgccgagc accggttcga
     3301 tcaggacgcc ggcgatctgc gtcggctcca cctggtggac caggacccat tcttcgaggt
     3361 agtcgaggta ccactggtcg tcgaatgggc cggggccgga atggaacggg gcccggaacc
     3421 ggttggggta gggcgcgaac accaggcccg cgacgtactg ggcgtgctgg gtggggacct
     3481 cggacaggtc ggtggacatg cccgcggtga ggtaggtgga ttcgccgtgg tactggccgt
     3541 agaacgacag gatcatggga cgtccggtgg cttcgcgcgc cagcttgacg ccggcttcca
     3601 cgaccagggt gccgctgacc cccatcgaga tcctcgtgag cccgggtggc gcgatctcta
     3661 ccaatcgctc agcgagggcc acgaccggtt cggtggcgac gtagttgctg atctccatgc
     3721 cgtaccggag ctgggcctcg gtcacggctc gctgcaccgc ctcgggcgtg gcgccgagcg
     3781 gcgtcgagcc ccaggccgag cagtggtcgg cgaacgtgtt gccgtccaca tcctcgatca
     3841 tccagtcgac cttgcgggcc tcgacgaacg gcacctcgcg gtgatcgagc gtgcccatgt
     3901 ggacggggtc gagacggtcg aacagcgctt ggctctgagg ccccggcagc tcggtccgca
     3961 cgaccgggac gtcgggtggt gcgacgttcg tctcgctcat cccgtcaccg gccggtcctc
     4021 gggaggcggc ggtggtgcgt ccgcgtcgtc gtaccagctg ccgccaccga gctctccacc
     4081 atcgacgatc aacgtctgcg ccgagatgaa ccgcgcgtcg tcgctggcca ggaacagcac
     4141 cgccatcgcc tggtcgcggg gggtgccggc gtactggagg gggatcttcg tcaggtagcg
     4201 ctgcaccagc tcccgttcgc gtgggtcctg tgccatgtcc tgctcggcct cgggggtgat
     4261 cgtctcgccg ggcgccacca cgttcgcgcg gatccggtga tggccgaact cgtgcgcgta
     4321 ggccttcgtg agcgccacca gcgccgcctt cgaggcgttg tagatcgact cgggggactc
     4381 gttctggaac gcgttcgcgc tggcgatgtt cacgatggct ccgggcgtgt cacgctcgat
     4441 ccacagccgg acgacgtcct gcatcaacat catcggcgcc cggacgttca ccgcgaacgt
     4501 cgcatcgaag tgctcggtgg aaacgtcgag cgcgtgctga tcgggttgca ggccggcgtt
     4561 gttgaccagc acgtcgaccc cacccagcag gttggcggcc gcgggtacga tcgtgcgcac
     4621 ctgatcggca tcggcgagat cggcggtatg gaccgccagg cgatccacgt ggggatgggc
     4681 gtcgcgcatc tgcgcgagtt cctcaccgag cacgtcggtc gccagaaccc gtgcgccttc
     4741 gtccaggaag gcgtccacga tgccacgccc gatcccgcgg gagccccccg tcacgatcac
     4801 tcctcgccct tcgaaccgca tcgtcgcgtt cgccccttcc tggtcgttgg tgccgatggt
     4861 catcgcagat cgccgacggc gacctgcgcg atcgtggcgg cttccaccat gtcctggtcc
     4921 agctcgatcc ccagacccgt gccgtgtggc acgtgtgccc tcccatccgg acccgtgtgg
     4981 atcgggttcc gcacggcgtg ctcccacggt tcgacgggca ccgcctgctc gaaatacgtg
     5041 gtgcgctcga aggccagcgc cacgtgcagg ttcgcggcct ggatcacggt gtggccgtag
     5101 ctgatcagtt caacgtcgat cccggcctcg gccgcgacgg ccatcaggga ccgagccgcc
     5161 gtgacaccgc ccgcgaagga cacgtcgaaa cgcatcgcgt cccacggcgg gtcgcgtagc
     5221 acctcggcca tgagccgggg atcccagaag gcgtcgccgg ccgcgatcac cggcacggcg
     5281 ggcacctgcc ggcgcaggtc gcggtagccc tgcagatcga agtcgggcag cgg
//
LOCUS       DATDBZ010000549         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_168517, whole genome shotgun sequence.
ACCESSION   DATDBZ010000549 DATDBZ010000000
VERSION     DATDBZ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168517"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VIX62_12685"
     CDS             <1..780
                     /locus_tag="VIX62_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY5861133.1"
                     /translation="GGDGSQALVASVASKHDVPHVVIPAGTRNHFALDLGLDRDDVVG
                     ALDAYGDGVERRIDLASVNGTTFVNNASMGLYAKIVQSPEYRDAKVQTVLDMLPDLLG
                     PDAVPLDLRYVGPDGEQQDSAHMLLVSNDPYQLDGGSGRGTRVRLDAGTLGVVSLRVK
                     SAAEAQRIVALDAVGKINRFSGWLEWSAERFQVDSGASVEVGVDGEALMMSPPLVFES
                     RPGALRVRIPRHALGASPAARGTSPLAFEDLAQVALGRSSG"
     gene            777..1406
                     /locus_tag="VIX62_12690"
     CDS             777..1406
                     /locus_tag="VIX62_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5861134.1"
                     /translation="MINDLRALPGAAQSLILIAAGVLVFVAALVLIRTGVVSWDERLF
                     TMLNTGTGSGSVGAKVVSAITTPIALVVLVVGAGFAVNRVHGSGAILVSAVAAAFGWA
                     LANGAKLLIDRPRPYLSLSEAILRQDPARGTSFPSSHTAIVTAVVVALLPLLPRPWRV
                     VGIIVILVVAWSRMYFGVHHPLDLLGGAGMGMAAAGLAWLIVGSIARAW"
     gene            complement(1413..1880)
                     /locus_tag="VIX62_12695"
     CDS             complement(1413..1880)
                     /locus_tag="VIX62_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5861135.1"
                     /translation="MQVADIMHTEVKSANAEDTFADVAKLMRTNGISSVVVLEGKKLG
                     GIVTERDIVNLVAAGGDPHSVKVAHGMTRRDLETIGPKMEIAEAAEHMATRNIRHLPV
                     VDRGKVVGIVSIRDLTKWAAEELAGGHEMPDIATSHKALQAASELQKQRRKGA"
BASE COUNT          271 a          703 c          747 g          376 t
ORIGIN      
        1 ggcggcgacg gctcccaggc cctcgtggcc tcggtggcga gcaagcatga cgttccccat
       61 gtggtgatcc ccgccggcac gaggaaccac ttcgcgctgg acctggggtt ggatcgcgac
      121 gacgtcgtgg gagcgctcga cgcctatggc gacggggtcg agcggcggat cgacctggct
      181 tcggtcaacg gcaccacgtt cgtcaacaac gcatcgatgg ggctctacgc gaagatcgtg
      241 cagtcgcccg aataccgcga cgcgaaggtg caaaccgtgc tcgacatgct gcccgacctg
      301 ctcggaccgg acgccgtgcc gctcgatctg cgctacgtcg gccccgacgg ggagcagcag
      361 gacagcgcgc acatgctgct ggtctccaac gatccgtacc aactcgatgg gggaagcggc
      421 cggggcacgc gggtgcgcct ggacgcgggc acgctcgggg tcgtgagcct gcgggtgaag
      481 agtgccgccg aggcacagcg tatcgtggcc ctcgacgccg tcggcaagat caaccggttc
      541 tccgggtggc tcgagtggag tgcagaacgg ttccaggtcg attccggagc gtcggtggag
      601 gtgggcgtgg acggcgaggc cctcatgatg agccctcccc tggtgttcga atcgaggcca
      661 ggtgccctgc gggttcgtat cccacggcac gcgctcgggg cctcgcccgc ggcgaggggg
      721 acctccccct tggcgttcga ggacctcgcc caggtcgcct tgggcaggtc gtccgggtga
      781 tcaacgatct ccgggcgctc cccggcgcgg cgcagtccct catcctgatc gcggccggcg
      841 tgctggtgtt cgtcgcggcg ctcgtgctca tccggaccgg cgtggtgtcc tgggacgagc
      901 gactcttcac gatgctcaac acgggcacgg gctcgggatc cgtcggagcg aaggtggtct
      961 cggcgatcac caccccgatc gcactggtgg tcctcgtcgt cggggccggg ttcgccgtca
     1021 accgcgtcca tggctcgggc gcgatcctcg tctcagccgt ggccgcggcg ttcggatggg
     1081 ccttggccaa cggggccaag ctcttgatcg accgcccccg tccctatctc tccctgtcgg
     1141 aggcgatcct gcggcaagac ccggcgcgag gcacgtcctt cccgtcgagt cacacggcca
     1201 tcgtgaccgc ggtggtggtg gccctgttgc cgttgctgcc tcgcccctgg cgggtggtgg
     1261 gcatcatcgt gatcttggtg gtcgcgtggt cgcggatgta cttcggggtg caccacccgc
     1321 tcgacctgct cggcggggcg ggcatgggca tggccgcggc gggcctcgcc tggttgatcg
     1381 tgggttcgat cgctcgcgcc tggtgatcga cgctatgcgc ccttgcgacg ctgcttctgg
     1441 agttcgctcg ctgcctgcag cgccttgtgg ctggtggcga tgtcgggcat ctcgtggcca
     1501 cctgccaact cttcggcggc ccacttcgtg aggtcgcgga tcgacacgat gccgaccacc
     1561 ttgccgcggt cgacgaccgg gagatggcgg atgttgcggg tggccatgtg ttcggccgcc
     1621 tcggcgatct ccatcttggg cccgatcgtc tcgaggtctc ggcgggtcat gccgtgggcc
     1681 accttgacgc tgtgcgggtc gccaccggcg gccaccaggt tcacgatgtc gcgctcggta
     1741 acgatgccgc cgagcttctt gccctccagc accaccaccg acgagatgcc gttggtgcgc
     1801 atgagcttgg cgacgtcggc gaacgtgtct tcggcgttgg ccgacttcac ctcggtgtgc
     1861 atgatgtctg cgacctgcat cgtcggtgcc tcctgaaggg atgacggctc gaagggtgga
     1921 agcctacctc ggcccggccg acctgcgtcc agccgtctca gtcgcgcttg aaccagcgac
     1981 ggacccggtc gaacaggccg ggctgccggt cgcgcgtcac accgggatcc gccggagccg
     2041 cttccttcgg gatcgacgct cccggaggca ccgccgcatg cggcggtgga ggagccg
//
LOCUS       DATDBZ010000550         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2233279, whole genome shotgun sequence.
ACCESSION   DATDBZ010000550 DATDBZ010000000
VERSION     DATDBZ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233279"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VIX62_12700"
     CDS             <1..704
                     /locus_tag="VIX62_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEY5861136.1"
                     /translation="GDEYEYYLAWQWVDITGIPAGTFVVRSKVDPYGYFTELSESNQC
                     WYTRVSFTSSSNAVTVHGSDNVCLDDWRDTAFAGDIQWAFDRGITAGCAPDLFCSNNR
                     VTREQMASFLVRALGLPPSATDHFVDDNASIHEADINALAASGITGGCGDGRFCPSAR
                     VQRGQMASFLVRAFDLPPSSSDFFTDDSASIHEGDINALAASGITAGCEAGRFCPGSP
                     VTRAEMVAFLHRAME"
     gene            complement(747..>2149)
                     /locus_tag="VIX62_12705"
     CDS             complement(747..>2149)
                     /locus_tag="VIX62_12705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5861137.1"
                     /translation="ARIIGIDGSLVWDEAVYAVRARAWTDPDAPVIGWDYIRPPLLPL
                     VSVPAVLAGGEDWMLRLVGLAAGAALLVLVWWLGRIIVGPLAGLAAAAVLYASPTLQW
                     HSGLALTDVPSAALLVALMVLLWRELQTKEQPGPGLTVAAVVAAAAFLTRYGAIAAIA
                     PILVVSVALWWRKIVRWPRWPSIAVAMLAVVAIGHAAWSISQTGSPFGVLSRSRDAVP
                     AWEWSPPFVMFQQWITLELAGTVGRLAVVVGFLAVPIAAAISVVDVRWHATLRGVTLI
                     ATVGISQVVLLVSGVAHVEQRYFVFGIAALVLAGACIAARLAMMVPVPFRIGLAVALG
                     LFVFASRGPSVDLAFDRTVSIARFYERFRLVGEEIGRLAGPDCGVVGAGGDPIVSWYS
                     GCSVVPLSPSLGGASPATLLDSEDAWLVRYARDGEVDTSSPALRAALDHVHEPGTEIR
                     DPTGGDLIAVIWPISP"
BASE COUNT          401 a          760 c          698 g          290 t
ORIGIN      
        1 ggggcgacga gtacgagtac tacctcgcct ggcaatgggt cgacatcacg ggcattccgg
       61 ccggcacgtt cgtcgttcgc tccaaggtgg acccgtacgg gtacttcacc gagctgagcg
      121 agtcgaacca gtgctggtac acccgggtca gcttcacctc cagctcgaac gcggtcaccg
      181 ttcacgggtc cgacaacgtg tgcctcgacg actggaggga caccgcgttc gccggtgaca
      241 ttcagtgggc cttcgaccgc ggcatcaccg ccgggtgcgc gccggacctg ttctgctcca
      301 acaatcgcgt cacccgtgag cagatggcca gcttcctcgt ccgcgcgctg gggctgccgc
      361 cgtcggctac ggaccacttc gtcgacgaca acgcctcgat ccacgaggcc gacatcaatg
      421 cactcgctgc gtcgggaatc accggcggat gcggcgacgg acggttctgt ccctcggcgc
      481 gggtccagcg cggccagatg gcgagcttcc tggtccgggc cttcgacctg cccccgagca
      541 gctcggactt cttcaccgat gactctgcgt cgatccacga aggcgatatc aatgccctcg
      601 ccgcatccgg catcacggcg gggtgcgagg ccggtcgctt ctgccccggc agccccgtga
      661 cgcgggcgga gatggtcgcg ttcctgcaca gggcgatgga gtagctgaaa cgcgttctcg
      721 tgggatcctc gggaccgagc gctcggtcag gggctgatcg gccagatcac cgcgatgaga
      781 tcgcctccgg tgggatcgcg gatctcggtc cccggctcgt ggacgtggtc aagcgcagcg
      841 cggagcgccg gactgctggt gtcgacctcg ccgtcacggg catacctcac gagccacgca
      901 tcctcgctat ccaggagcgt ggccggggaa gcacctccga gcgacggact cagcggcacc
      961 accgagcagc ccgagtacca ggagacgatc gggtcgccgc cagcgccgac cacaccgcag
     1021 tcgggcccag ccaagcgtcc gatctcctct ccaaccaatc ggaaccgctc atagaagcgg
     1081 gcaatgctga ctgttcgatc gaaggcgagg tcgactgacg gtccgcgact ggcgaagacg
     1141 aacaggccca gggcgaccgc caggccgatg cgaaacggga ctggcaccat catcgccagc
     1201 cgcgccgcga tacaggcgcc cgcgagcacg agggccgcga tcccgaagac gaagtaccgc
     1261 tgctcgacgt gggccacccc tgacacgagg aggacgacct gtgagatacc caccgttgcg
     1321 atgagcgtga ccccacgcaa ggtggcatgc catcgcacgt ccaccacgct gattgccgcc
     1381 gcgatcggaa ccgcgaggaa tcccacgacg acggcgagcc ggccgaccgt tccggccagc
     1441 tcgagcgtga tccactgctg gaacatgacg aacggagggg accactccca cgccggcacc
     1501 gcatcgcgag acctgctgag caccccgaat ggcgagccgg tttggctgat cgaccacgcc
     1561 gcgtgcccga ttgccacgac cgccagcatc gcgaccgcga tcgacggcca gcgtggccag
     1621 cggacgatct tccgccacca gagcgcaacg gatacgacga ggatcggcgc gatggcagcg
     1681 atcgccccgt agcgagtgag aaaggcggcc gctgcgacca cggcggcgac cgtcaaccct
     1741 ggaccaggct gctctttcgt ctggagctcg cgccacagca ataccatcag cgcgacgagg
     1801 agggcggccg aaggcacatc ggtgagtgca agcccgctgt gccactgcag cgtcgggctg
     1861 gcgtagagaa cggccgccgc cgcgaggccg gccaacggcc cgacgatgat ccgccccagc
     1921 caccacacga gcacgagcaa cgccgcgccc gccgcaagac cgaccagccg gagcatccag
     1981 tcctcacctc ccgccaggac ggcaggaacg ctgacgagcg gcaacagggg cggtcggatg
     2041 tagtcccagc cgatgaccgg agcatcagga tcggtccagg cccgtgcacg caccgcgtag
     2101 acagcctcgt cccagaccag tgagccgtcg attccgatga tccgtgcca
//
LOCUS       DATDBZ010000551         2971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2011124, whole genome shotgun sequence.
ACCESSION   DATDBZ010000551 DATDBZ010000000
VERSION     DATDBZ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011124"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..2365
                     /locus_tag="VIX62_12710"
     CDS             212..2365
                     /locus_tag="VIX62_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5861138.1"
                     /translation="MAAFDAPTTTTAGVEAPLASIFTAQVESRVLDHTARWLRAQGHG
                     YYTIGSSGHEGNAAVAAALRLTDPALLHYRSGGFYCARAQQRPGHQAARDVLAGMLAL
                     TEDPISGGRHKVFGHHALGVIPQTSTIASQLPRALGVAFAIGRARRLRLTTPWAVDAL
                     AVCSFGDASANHSTAQGAINAACYVSHSGLPIPLLLVCEDNGWGISVPTPAGWIEASF
                     GHRPGLRYAQVDGADPQAVYDTTEQLAAHVRDTARPAFLHLRTVRFMGHAGTDVESSY
                     RSAAAIRDDQARDPILGTAMAMVRTGLATPDDLVDRYLSARASVRSLALEMIHAPTFD
                     NADDVVRMLSPRRPGRVAEIASCAGAAADREAAFDGALPEHEGQLTFAETINRTLVDV
                     LAATPNALVFGEDVAVKGGVYGVTRGLQKRFGAARVFDSLLDEQSILGLALGAGVSGM
                     LPIPEIQYLAYLHNAEDQLRGEAASLSFFSSGQYRNPLVVRIAGYGYQKGFGGHFHND
                     NSVAVLRDIPGLVIASPGHPSDAAPMLRTCVAAALSDGTVCAFLEPIALYHTRDLFEV
                     DDGAWTATYMAPDRWAAQHVPIGEGRLVREGDDVLIVSWANGLAMSLQAARQLSAEGI
                     SCRVFDLRWLAPLPIEQVLAHAIQTDRVLIVDETRHSGGVGEGIVTGLIEAGYRGSIE
                     RVAALDSFVPLGDAANLVLVSHDQIVEAARRLPIR"
     gene            2630..2746
                     /locus_tag="VIX62_12715"
                     /pseudo
     CDS             2630..2746
                     /locus_tag="VIX62_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580042.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          437 a         1024 c         1009 g          501 t
ORIGIN      
        1 cggatcacga gagcagctca gtgccggtca acggaccgcg atcgtaggtt gcgtcagagc
       61 aatggtggcg gatccccttg tgctcgtgcg tcgctcatcg ggtgatgggg tgccccggtc
      121 gtgacgtcgt cgatgctcct acgatccagg cgtgaccggt gccgtgttgc ccgacgacct
      181 ccacgaactc gaacggtcgc tgctcagcgc tatcgcggcg ttcgacgcgc cgacgacaac
      241 gaccgccggg gtcgaggcac cattggcgag catcttcacc gcacaggtcg agagccgtgt
      301 gctggatcac accgcccgat ggctgcgcgc ccaggggcac ggttactaca cgatcggctc
      361 gagcggtcac gagggaaacg ctgcggtggc ggctgctctc cgcctgaccg accctgccct
      421 gctgcactac cgctcgggag ggttctactg tgcccgcgcg cagcagcgac ctgggcacca
      481 ggcggctcga gatgtgttgg ccgggatgtt ggcgctcacc gaggatccga tctccggtgg
      541 ccgccacaag gtgttcgggc accacgcact cggagtgatt ccacagactt ccacgatcgc
      601 ctcgcagctg ccgcgggcgc tcggtgtggc gttcgcgatc ggtcgggcac ggcggctcag
      661 gttgacgacc ccgtgggcag ttgacgctct tgccgtgtgc tccttcggtg atgccagcgc
      721 caaccattcc acggcccagg gggcgatcaa cgccgcctgt tacgtgtcgc acagtgggtt
      781 gccgatcccg ctgctgctgg tgtgcgagga caacggctgg gggatcagcg tcccgacgcc
      841 ggccggctgg atcgaggcct cgttcggcca ccggccgggc ctgcgctacg cacaagtcga
      901 cggggccgat ccacaggcgg tctacgacac caccgaacaa ctcgccgccc acgtgcgcga
      961 caccgctcgg ccggcgttcc tgcaccttcg caccgtgcga ttcatgggcc acgccggaac
     1021 cgacgtcgaa tcgagctatc gatctgccgc cgcgatccgt gacgatcagg cccgcgaccc
     1081 gatcctcggc acggccatgg cgatggtgag aaccggtctg gcgactcctg acgatctggt
     1141 cgaccggtac ctgtcggcgc gcgcctcggt tcgctcgctg gcgctcgaga tgatccacgc
     1201 cccgacgttc gacaatgccg acgacgtggt gcggatgttg tcgccgcgtc gccctggccg
     1261 ggtggcggag atcgcctcat gtgccggtgc ggccgcggat cgcgaggcgg ccttcgacgg
     1321 cgcattgccc gaacacgagg gccaactgac gttcgccgaa acgatcaacc gcacgctcgt
     1381 cgacgtcctg gccgccacgc ccaatgcact ggtgttcggc gaggacgtcg cggtgaaagg
     1441 tggtgtgtac ggcgtgaccc gcgggctgca gaagcggttc ggcgcggcac gggtcttcga
     1501 ctccctgctc gacgagcagt cgatcctcgg gctggccctc ggcgcggggg tgtcggggat
     1561 gctgccgatt ccggagatcc aatacctcgc ctatctccac aacgccgagg atcagttgcg
     1621 cggcgaagcg gcgagcctgt cgttcttctc cagtgggcag taccgcaacc cgctcgtcgt
     1681 acgcatcgcc ggctacggct accagaaggg attcggcggg cacttccaca acgacaactc
     1741 cgtggccgtg ttgcgtgaca tcccgggtct cgtgatcgcc agcccgggtc atccgtccga
     1801 cgcggcgccg atgttgcgga cctgcgtggc cgctgcgctc agcgacggca cggtctgcgc
     1861 ctttctcgag ccgatcgcgc tctaccacac ccgcgacctg ttcgaagtcg acgacggcgc
     1921 ctggaccgcc acgtacatgg cacccgatcg gtgggctgcc cagcacgtgc cgatcgggga
     1981 aggccgcctg gttcgtgagg gcgatgacgt gttgatcgtg agctgggcga acggtctggc
     2041 gatgtcactc caggcggccc gccaactctc ggccgagggc atctcgtgcc gggtcttcga
     2101 cctgcggtgg ctggcgccac tgccgatcga gcaggtgctc gcccacgcca tccagaccga
     2161 ccgggtgctg atcgtcgacg agacccgcca ttccggtggc gtcggggagg ggatcgtgac
     2221 cgggctgatc gaggccggct accgcggctc gatcgagcgg gtcgccgcgc tcgactcgtt
     2281 cgtgccactc ggcgacgccg ccaatctggt gctcgtcagc cacgaccaga tcgtcgaggc
     2341 cgcccggcgc ctgccgattc gttgacccgc caatggcgag tccgtgcaac gcgtggtggc
     2401 ccatcggatg atcgcgatca aacatgctgg gatgtccgcg gacgcagtgc acgtcgaggt
     2461 gctcggcccc ttgcggacgc gcgggtcgaa gcggcgcggc aggaggacga gaccatggtc
     2521 accgtgattc gaccattcat tccggctgac gccgaccgca tcgtcgagct ctcgctcgcc
     2581 gcatggcagc ccgtcttctc gtcgtccgcg acgtgctcgg gcccgcaacc tcgatctcgc
     2641 catcgtcgcc accggcggtg accccggaca cgcgccagcc cggcgaacct acgagcgagc
     2701 agggttcacc gggtgtcccc aagtctggta cgccaaactc ctgtgacgtc cgaccgtcgt
     2761 gacccggcat tcgggccagg ttcgctcgtg tcccggtcag gtcgcggcgg aggcgctgga
     2821 gacgatgact gcgccaccgc gaaagacaga caccgggccg tcctcgctga ccaccacgat
     2881 tgtcgccgtg cggtggtcgt ggctgtatcg ctgggcggat cggtggcgca tcccgcggtc
     2941 gctggtcacc gcgccctcgg cgtccggggt c
//
LOCUS       DATDBZ010000552         2013 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_585413, whole genome shotgun sequence.
ACCESSION   DATDBZ010000552 DATDBZ010000000
VERSION     DATDBZ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585413"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VIX62_12720"
     CDS             <1..1065
                     /locus_tag="VIX62_12720"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY5861139.1"
                     /translation="MHGDPGWAQTLVFFLLFAPAWWAWVNMTLTLNLFGARVTPLVWV
                     AVTVAMIAIGVMAAAVPEALTERAWAFAVGNAVIRLVWMLPWVVKRRTIGVPWWRPIA
                     YSGVPAALWLVSAVVPPPAQYVLWVVAIAVEVVLLGSLGGQATWLQRSLDVDHLLERV
                     SLLVVIVFGESILATIAELDAHWTLTSGLTAVLAFLAISLLAWVFFRYASGSAEAGLR
                     MLQSAGRIGALRDAVMYLPFILIAGIALFASGLGTAVAEAGHTLAPGAAVCIAGGIAL
                     FYVASIAESLRYVVPWRDVVTWGIAGVVLPWVIPPLAAVVDAGAVVAVVVVLIVVMIG
                     LAEANLRRMRAARENAARSR"
     gene            complement(1218..>2013)
                     /locus_tag="VIX62_12725"
     CDS             complement(1218..>2013)
                     /locus_tag="VIX62_12725"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY5861140.1"
                     /translation="FEEGRHVVLRGAVAADGTDADAPYDFTWAFVLIDGGPGTTRLVV
                     RERYAYLTGWAGALVEPVEMVSFLMSQRMLRGIRERAERGTSAGPSNPPTVMGAIADD
                     ENPPVSNVRERRPTVPPRWFVRLAWRVHRGMYQITGGRVGLWRPKPQGWGTLHLTTTG
                     RRTGHDREVILGYFEDGPNLVTMAMNGWADPEPAWWLNLQAQPDATVVVRDGPRLVHA
                     RAAHGDERSRLWSRWREIDTGLDELAALRSSETAVVILEPRSAPKP"
BASE COUNT          263 a          688 c          700 g          362 t
ORIGIN      
        1 atgcatggcg accccggctg ggcccagacg ctcgtgttct tcctgctgtt cgcgccggcg
       61 tggtgggcat gggtgaacat gaccctcacg ctcaacctgt tcggtgctcg cgtcacaccg
      121 ttggtctggg tcgctgtcac ggtcgcgatg atcgccattg gagtcatggc ggccgcggtt
      181 cccgaggcgc tcaccgagag ggcatgggcc ttcgcggtcg ggaacgccgt catccgcctc
      241 gtctggatgc tgccgtgggt cgtgaagagg cgcaccatcg gagtgccgtg gtggcgcccg
      301 atcgcctaca gcggagtccc cgcggcgctc tggctcgtct cggcggtcgt tcccccgccc
      361 gcgcagtacg tgctgtgggt ggtcgcgatc gccgtcgaag tcgtgctgct cggctccctc
      421 ggcggtcagg cgacgtggtt gcagcgctct ctcgacgtgg atcacctgct cgagcgggtg
      481 agcctgctcg tcgtcatcgt gttcggggag tcgatcctgg cgaccatcgc ggagctcgat
      541 gcgcactgga cgctcacttc gggcctgacc gcggttctcg cgttcctcgc catctcactg
      601 ctcgcctggg tgttcttccg ctacgcgtcc ggttcggcgg aagccggctt gcgcatgctc
      661 cagtcggccg gccgtatcgg ggccctgcgg gacgcggtga tgtacctgcc gttcatcctg
      721 atcgcgggca tcgcgctgtt cgcctctgga ctcggcactg cggtcgccga agccggacac
      781 accctcgcgc ccggcgcggc cgtctgcatc gcgggcggga tcgcgttgtt ctacgtggcg
      841 agcatcgcgg agtcccttcg ctacgtggtt ccgtggcgcg acgtggtgac gtgggggatc
      901 gccggcgtcg tcctgccatg ggtgatcccc ccactcgccg ccgtggtcga tgcgggggcc
      961 gtggttgccg tggtcgtcgt gctcatcgtg gtgatgatcg gcctcgccga ggcgaacctc
     1021 cgtcgcatgc gcgccgcgcg agagaatgcc gcgaggtcac gatgagcggc gcacgaccct
     1081 cgaatgccgg acccgatgcg atcgcggctc cgtcgcgcgg gcgggtcgga accggatcgt
     1141 catggcgggc ccctgagccc gcactcgacc cgcggcgtga cctcgacgta cacggttcgt
     1201 gagatgaggc cgtcgcatca gggtttcgga gcggaacgtg gctccaggat cacgactgcg
     1261 gtctcggacg accgaagcgc cgcaagctcg tccagccccg tgtcgatctc acgccatctg
     1321 gaccagagac gcgagcgttc gtccccgtgc gcggcgcgag cgtgcaccag gcggggcccg
     1381 tcgcgaacga cgaccgtcgc atcaggttgg gcttggaggt tgagccacca tgccggctcg
     1441 gggtctgccc atccgttcat ggccatcgtg acgagattcg gaccgtcttc gaagtagccg
     1501 aggatcacct ctcggtcatg cccggtgcgc cgccccgtgg tggtcaagtg gagcgtgccc
     1561 cacccctgag gtttcggacg ccacaacccg actcgtccac cggtgatctg gtacatgccc
     1621 cgatggaccc tccaggcgag gcggacgaac cagcgaggcg gaacggtcgg tcgtcgctct
     1681 cgtacgttcg acacgggtgg attctcgtcg tcggcgatcg ctcccatgac cgtcggcgga
     1741 ttcgagggcc cagccgacgt gccgcgctca gcgcgctccc ggatgccgcg gagcatccgt
     1801 tgcgacatga ggaagctcac catctcgacg ggctcgacga gtgcgcccgc ccagccggtc
     1861 agatacgcgt agcgctcgcg cacgacgagg cgtgtggtgc ccggtccacc gtcgatgagg
     1921 acgaacgccc acgtgaagtc gtagggggca tcagcgtcgg tgccgtcggc agcgaccgcg
     1981 ccccgcagca cgacgtgccg cccctcctcg aac
//
LOCUS       DATDBZ010000553         3135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2421638, whole genome shotgun sequence.
ACCESSION   DATDBZ010000553 DATDBZ010000000
VERSION     DATDBZ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2421638"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VIX62_12730"
     CDS             <1..443
                     /locus_tag="VIX62_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY5861141.1"
                     /translation="EEALTLSDRIAVFNAGRIEQLGTPRELYEQPGSRFVADFVGTSN
                     LFDLDRSTQLIGRAGEHTIRPEKLTLSLQPGSGEGVRSAPGTIVESIYVGSAIRHVVD
                     LDAGGRVTVLERNDRSRAADQDRGDRVHVSWHDSDVVALIPPGT"
     gene            500..1678
                     /locus_tag="VIX62_12735"
     CDS             500..1678
                     /locus_tag="VIX62_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018173508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5861142.1"
                     /translation="MRHITRGARSSAVAALAIGAVVALTACGTSSGGGTASEEATELG
                     EMEGSVSILAWPGYVEDGSNDPAVDWVSGFEDETGCMVESKTYGTSDEAVSLMKTGEY
                     DVVAASGDATLRLIAGGDVAPVNTDLIPSYAGIYDFLKDQAWNSVDGVSYGVPHGYGA
                     NLLLYNTDVVTPAPTSWDVVFDKAADYTGKVTAYDSPIYIADAAVYLMAHQPDLGIEN
                     PYALDEEQFQAAVDLLKEQRPHISEYWSDYLKEISAFETTDSVVGTTWQVIQNVLESE
                     SAPTAVVLPEEGATGWSDTWMIASEAKSPNCAYAWLDWIASPETNAAATAYFGEAPSS
                     QDACDYREDCEAYHAGDEEYASQIWYWTTPIAECVDGRTDVECVDYSKWTEAWSEIKG
                     "
     gene            1743..2672
                     /locus_tag="VIX62_12740"
     CDS             1743..2672
                     /locus_tag="VIX62_12740"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5861143.1"
                     /translation="MTATVTPAEERPTRAVPPPRTTAPRRLSSFLTAHPRTRLALLLT
                     APLFWLGVVYIVALVLLLVTAFWQVDSFTGEIITEWTLDNIITVLTGALYQTVTVRTL
                     GVALMVTLIDVLLALPIAFYMAKVASPRAQRILLIAVLMPLWASYLVKAYAWRSVLSQ
                     DGILEWLLAPLGLHTPGYGLTATIITLSYLWLPYVILPIYAGLERVPDSLLEASADLG
                     GKTWRTLGSVVLPLVFPAIIAGTIFSFSLSLGDYITVNIVGGANQMLGNLVYTNVGAA
                     NNLPLAAAIALIPIVIIFGYLAIVRRTGALNNL"
     gene            2681..>3135
                     /locus_tag="VIX62_12745"
     CDS             2681..>3135
                     /locus_tag="VIX62_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5861144.1"
                     /translation="MRLGRTARIVLAAVTGLILVAIYVPLMVVLVNSFSTSTSLTWPP
                     PGFTLEWWGKAFESAGAREAVLTSVQVAIVATVISLVLGTLISIALQRFEFFGRDAIS
                     LLIILPIALPGIITGIALNNFFRTIMGIPLSIWTVVIAHATFCIVTVFN"
BASE COUNT          517 a         1092 c         1016 g          510 t
ORIGIN      
        1 aggaggaggc gctcaccctc tccgatcgca tcgccgtgtt caacgccggc cggatcgagc
       61 agctgggcac cccgcgcgag ctgtacgagc agccgggatc ccgtttcgtg gccgacttcg
      121 tagggacctc gaacctcttc gacctcgacc gctcgaccca gctcatcggc cgcgcgggcg
      181 agcacacgat ccgccccgag aagctgaccc tgtcgctgca gccgggctcg ggggagggcg
      241 tgcgctcggc gccgggcacc atcgtcgagt cgatctacgt cggcagcgcg atccgtcacg
      301 tcgtcgatct ggatgccggt ggacgggtga ccgtactgga gcgaaacgac cgttcacgcg
      361 ccgctgacca ggaccgcggc gatcgcgtac acgtgagctg gcacgactcg gacgtcgtcg
      421 ccctcatacc cccgggaacc tgacccggga aaacagccgt gggcactcgc cccggcgctt
      481 ggcaccgaga ggaagaacaa tgaggcacat cacacgcgga gcgcgttcca gcgcggtcgc
      541 cgccctggcg atcggcgcgg tcgtcgcgct gacggcctgc ggcaccagct cgggcggtgg
      601 caccgcaagc gaggaagcga ccgagctcgg ggagatggaa ggctcggtct cgatcctcgc
      661 ctggcccggc tacgtcgagg acggcagcaa cgaccccgcg gtcgactggg tgtcgggctt
      721 cgaggacgag accggctgca tggtggagtc caagacctac ggcacctccg acgaggcggt
      781 cagtctcatg aagacgggcg agtacgacgt ggtcgcggca tccggcgacg ccactctccg
      841 cctcatcgcc ggcggcgacg tcgccccggt gaacaccgac ctgattccga gctacgcagg
      901 catctacgac ttcctgaagg atcaggcgtg gaactcggtg gacggcgtct cgtatggcgt
      961 gccccacggc tacggcgcca acctgctgct gtacaacacg gacgtcgtga ccccggcgcc
     1021 gacctcgtgg gacgtcgtgt tcgacaaggc cgccgactac accggcaagg tcaccgcgta
     1081 cgactcgccg atctacatcg cggatgccgc ggtgtacctg atggcgcacc agcccgacct
     1141 cggcatcgag aacccgtacg cgctcgacga ggagcagttc caggccgccg tcgacctgct
     1201 caaggagcag cgcccgcaca tcagcgagta ctggtcggac tacctgaagg agatctcggc
     1261 gttcgagacc accgactcgg tggtcggcac gacgtggcag gtcatccaga acgttctcga
     1321 gagcgagagc gcgccgaccg cggtcgtgct gcccgaagag ggcgcgaccg gctggtcgga
     1381 cacgtggatg atcgcatccg aggcgaagag cccgaactgc gcatacgcgt ggctcgactg
     1441 gatcgccagc ccggagacga acgccgcagc gaccgcgtac ttcggcgagg cgccgtccag
     1501 ccaggacgcg tgcgactacc gcgaggactg cgaggcgtac cacgccggtg acgaggagta
     1561 cgcgtcgcag atttggtact ggacgacccc gatcgccgaa tgcgtcgacg gccgcacgga
     1621 cgtcgaatgc gtcgactact cgaagtggac cgaagcctgg tccgagatca agggctgacc
     1681 cacaccgaac accgggggcg gcggacccga accgtcgccc ccggtttcca cccggagcac
     1741 acatgaccgc gaccgtgaca cccgccgagg agcgcccgac gcgggcggtt ccgccgccgc
     1801 gcaccacggc gccgcgccgg ctctcgtcgt tcctgacggc gcacccgcgc acccgcctcg
     1861 cactgctgct gaccgccccg ctgttctggc tcggcgtggt ctacatcgtc gcgctcgtgc
     1921 tgctgctggt cacggcgttc tggcaggttg actcgttcac gggcgagatc atcaccgagt
     1981 ggaccctcga caacatcatc accgtcctga ccggcgccct gtaccagacg gtcacggtgc
     2041 gcaccctcgg ggtcgcgctc atggtgaccc tgatcgatgt cctgctggcg cttcccatcg
     2101 cgttctacat ggcgaaggtg gcctctccgc gagcgcagcg catcctgctg atcgcggtgc
     2161 tgatgccgct gtgggcgagt tacctggtga aggcgtacgc gtggcgatcc gtgctctcgc
     2221 aggacggcat cctggaatgg ctgctggccc ccctcggcct gcacacgccc gggtacgggc
     2281 tcacggcgac catcatcacc ctctcgtacc tctggctgcc gtacgtgatc ctgccgatct
     2341 acgcgggtct cgaacgggtg cccgactctc tgctggaggc atctgcagac ctcggcggaa
     2401 agacgtggcg gacgctgggg agcgtcgtgc ttccgctggt gttcccggcg atcatcgccg
     2461 gcacgatctt cagcttctcg ctctcgctcg gcgactacat cacggtcaac atcgtcggcg
     2521 gtgcgaacca gatgctcggc aacctcgtct acacgaacgt cggcgcggcc aacaacctcc
     2581 ctcttgcggc ggcgatcgcg ctcatcccga tcgtgatcat cttcggctac ctcgcgatcg
     2641 tgcgccgcac gggcgcgttg aacaacctct aggacgacgc atgcgactcg gaagaaccgc
     2701 acggatcgtg ctcgcggccg tcaccggcct catcttggtg gcgatctacg tgccgctgat
     2761 ggtggtgctg gtcaactcct tctccacctc gacctcgctg acctggccgc cgccgggctt
     2821 cacgctcgag tggtggggga aggcattcga gagcgcaggc gcccgtgagg cggtgctgac
     2881 cagcgtgcag gtcgcgatcg tcgcgacggt catctcactc gtgctgggca cgctcatctc
     2941 gatcgcgctg cagcgattcg agttcttcgg ccgcgacgcg atcagcctgc tgatcatcct
     3001 gccgatcgcg ctgcccggca tcatcaccgg catcgccttg aacaacttct tccgcacgat
     3061 catgggcatt ccactgtcga tctggacggt ggtgatcgcg cacgcgacct tctgcatcgt
     3121 gacggtcttc aacaa
//
LOCUS       DATDBZ010000554         3851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2643933, whole genome shotgun sequence.
ACCESSION   DATDBZ010000554 DATDBZ010000000
VERSION     DATDBZ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643933"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..984
                     /gene="proC"
                     /locus_tag="VIX62_12750"
     CDS             148..984
                     /gene="proC"
                     /locus_tag="VIX62_12750"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY5861145.1"
                     /translation="MSTPQTRRVAFLGGGKMAEALISGLIRSGGRTVDEIMVTNRREE
                     RAQELAGKYGVTATLDNGEAVRWADVLVLMAKPQDTETLLSQIREHVTTDDTVISFAA
                     GVRTSFVEKHLPDGVPVVRVMSNVPVMVDEAMSVISSGDIATDEHMAIAEELLGYVGR
                     VLRLKEQHLDAVTATSGSGPAYFFLLAEAMIEACILLGLSRDVATDLIIQTMLGSAKM
                     LRDTGKHPVELREMVTSPGGTTIAAIRHLEEAGVRAAFLNAIDAARQRSLELAQGGDD
                     DD"
     gene            1022..1147
                     /locus_tag="VIX62_12755"
     CDS             1022..1147
                     /locus_tag="VIX62_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861146.1"
                     /translation="MGRGSLAKVRSAKDRQRKKKAREVRQAADRAAARKAAKKKR"
     gene            complement(1148..1735)
                     /locus_tag="VIX62_12760"
     CDS             complement(1148..1735)
                     /locus_tag="VIX62_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY5861147.1"
                     /translation="MFTIASSFDSLFTMPMPIGEKVIRTIAVYLIVVVLLRIMGRRTV
                     AQLNAFDLVVLLLLSNVVQNAIIGPDNSLVGGAIGVVTLLLLNAAVVWVVRKNSWLDK
                     VMEGDTVVLVHDGKIDHHALQHLGIREGDLTAALLNQGAEGVHDVHEAEMYPSGSIVV
                     ELTEQARDASRMDLDRVEHKIDELAAAVARLSPTS"
     gene            complement(1759..2547)
                     /locus_tag="VIX62_12765"
     CDS             complement(1759..2547)
                     /locus_tag="VIX62_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5861148.1"
                     /translation="MRLVTYDRGGHRRLGAILEAEVIDLPDAVGHPAFPTTLEGLVSS
                     SRGTVMDAARAALQRDDAWNWRVPKPRVLTPLFPQSLLSPRAMDVDRRIVGPEQAVPW
                     PEEAAWLDYEPMVAAVVGNEGAKLEADAVGDHVFGYTLVSDWRAHASDGAPVEHADGV
                     PIAIGPCVVTTDELDPQTMFVTVKVDDQDLLKGNLNGAAASLFRLIADVSQHAVLERG
                     DAFALGPFPPVEDDADPSRRLWPGAQVELAAEGIGTLRNRVDAR"
     gene            2745..>3851
                     /locus_tag="VIX62_12770"
     CDS             2745..>3851
                     /locus_tag="VIX62_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5861149.1"
                     /translation="MAEKFRITYATMSADNEELQAAYDAAAERARAELGKEYPVVVNG
                     EERWRDEKYEEPSPIDADITIGKFSQATTQDVDDAVAAAKAFQLEWDRMGWQERVRIL
                     RNVADIMEDRVYDLGALMAYEVGKSRLEALGDVQETAELIRWNCDEMEKHDGFRVPMS
                     GLGAAGDYYDVLRPHGVWAVISPFNFPMALSGGPSSGALVAGNTVVLKPSNQGALLGY
                     KLYECYRDGGVPAGAFHLVTGRGAVAGDHLWHHPDVNGITFTGSYPVGMDIYKHFSTD
                     VPKPVICEMGGKNPTIVTKNADLDKATEGVLRSAFGFGGQKCSACSRVYVEREVYDDF
                     VTQLKTKTEKIKVGNPIERDVYMGPIINEPAMETY"
BASE COUNT          660 a         1264 c         1358 g          569 t
ORIGIN      
        1 ggccgagctc gatccgggcc tgatccatcg ccaacccgag cgccggcaga tgcggatcaa
       61 ggagttcctg gaggtcctgc gcagcggcat cgaggcgcag gccgaggtct gagacccggg
      121 cgtcgcgtgc ggtaccgtga acctctgatg agcacgccgc agacacgacg cgtcgccttc
      181 ctgggcggag gcaagatggc cgaggccttg atctcgggcc tgatccgctc gggcggccgc
      241 accgtggacg agatcatggt gacgaaccgc cgcgaggagc gcgcacagga gctggccggt
      301 aagtacgggg tcaccgcgac gctcgacaac ggcgaggccg tccgatgggc cgacgtgctg
      361 gtgctcatgg ccaagccgca ggacaccgag accttgctgt cccagatccg cgagcacgtc
      421 acgaccgacg acaccgtgat cagtttcgca gcaggcgtcc ggacctcgtt cgtcgagaaa
      481 caccttccgg acggcgtgcc cgtggtgcgc gtgatgtcga acgttccggt catggtcgac
      541 gaggcgatgt ccgtgatcag ttccggcgac atcgccaccg acgagcatat ggcgatcgcc
      601 gaagagctcc tcgggtacgt gggacgggtg ctgcgcctga aggaacagca cctcgacgcg
      661 gtcaccgcca ccagcggcag cgggcccgcc tacttcttcc tgctggccga ggcgatgatc
      721 gaggcctgca tcctgttggg actctcgcgt gacgtcgcca ccgacctcat catccagacg
      781 atgctgggtt cggcgaagat gctgcgcgac accggcaagc acccggtgga gctgcgtgag
      841 atggtgacct cgcccggcgg caccacgatc gcggcgatcc gtcacctgga agaagccggg
      901 gtgcgagccg ccttcttgaa cgcgatcgac gccgcgcgcc aacgctcact cgagttggcg
      961 cagggtggcg acgacgacga ctagccggcc gggtccgatc ggccgcaagg aaggaccaca
     1021 gatgggcagg ggatcattgg ccaaggtgcg ctccgcgaag gaccgccaac gcaagaagaa
     1081 ggcgcgcgag gtgcggcagg ccgccgaccg ggccgccgcc cgcaaggccg cgaagaagaa
     1141 gcggtagtca ggacgtcggg ctcaggcgcg ccaccgctgc ggcgagctcg tcgatcttgt
     1201 gctccacccg gtccagatcc atcctgctcg catccctggc ctgctcggtg agctccacga
     1261 cgatcgagcc agaggggtac atctcggctt cgtgcacatc gtgcacccct tctgcgccct
     1321 ggttgagcag cgcggccgtg agatcgccct ctcggatccc gaggtgctgg agcgcgtggt
     1381 ggtcgatctt gccgtcgtgc accaggacca cggtgtcgcc ctccatcacc ttgtccagcc
     1441 aggagttctt gcgcaccacc cacaccacgg ccgcgttgag caggagcagc gtgaccacgc
     1501 cgatcgcgcc gcccacgagt gagttgtccg ggccgatgat ggcgttctgc accacgttgc
     1561 tgagcagcag cagcacgacc aggtcgaagg cgttgagttg ggccacggtt cggcggccca
     1621 tgatccggag cagcaccacc acgatcaggt agacggcgat cgtgcggatc accttctcgc
     1681 cgatcggcat cggcatcgtg aacaggctgt cgaacgaact cgcgatcgtg aacatgggtc
     1741 ctcctcgggc cggaagcttc agcgggcgtc gacgcggttc cgcagcgtgc cgatgccctc
     1801 ggcggcgagt tccacctgag cgccaggcca cagtcggcgc gaaggatcgg catcgtcctc
     1861 gaccggcggg aacggaccga gcgcgaaggc gtcgcctcgt tccaggaccg cgtgctggct
     1921 gacgtcggcg atcaggcgga acaggctggc ggcggcgccg ttcaggttgc ccttcaacag
     1981 gtcctggtcg tcgaccttga ccgtgacgaa catcgtttgg ggatcgagct cgtcggtggt
     2041 gacgacgcac ggcccgatcg cgatggggac gccgtcggcg tgttcgaccg gggcgccgtc
     2101 ggacgcgtgc gcccgccagt cgctcaccag ggtgtagccg aagacgtggt cgccgacggc
     2161 gtcggcctcg agcttcgcgc cctcgttgcc gaccacggcg gccaccatgg gctcgtagtc
     2221 gagccaagcg gcttcctcgg gccacggcac cgcttgctcg gggccgacga tccggcggtc
     2281 gacgtccatg gcgcgggggg agagcaggga ctgggggaac aggggcgtga ggacgcgagg
     2341 cttcgggacg cgccagttcc acgcgtcatc tcgttgcagg gctgcacggg cggcatccat
     2401 gacggtgcct cggctggacg aaaccaggcc ctcgagggtg gtggggaacg ccgggtgccc
     2461 gacggcatcc ggcagatcga tcacctctgc ctcgaggatc gctcccagtc gccggtgccc
     2521 cccccggtcg taggtcacga gcctcatgtg ggagccgaag catacgcccg tcccgggccg
     2581 atttccgggt ttcgggtcgg cagtatcccc cgcgggccgg cggcaggggc caaccggcgg
     2641 tcagcgcagg ccatgggcgg gtttcggggc gctcggctag catgtcggcc tgcgccggca
     2701 ggcagagaga ggcctgcgcg ctccgagaag gggagggact cccgatggct gagaagttcc
     2761 ggatcactta cgccacgatg tcggccgaca acgaggaact gcaggcggcc tacgacgctg
     2821 ctgccgagcg cgcccgcgcc gaactcggca aggagtaccc ggtcgtcgtc aacggcgaag
     2881 agcgctggcg tgacgagaag tacgaggagc cctcgccgat cgatgccgac atcacgatcg
     2941 gcaagttcag ccaggccacc acgcaggacg tcgacgacgc cgtggcggct gcgaaggcat
     3001 tccagctgga gtgggaccgc atgggttggc aggagcgcgt gcgcatcctg cgcaacgtcg
     3061 cggacatcat ggaggaccgc gtctacgacc tgggcgccct catggcctac gaggtcggca
     3121 agagccggct cgaggcgctc ggcgacgtgc aggagaccgc ggagctgatc cgctggaact
     3181 gtgatgagat ggagaagcac gacggcttcc gggtcccgat gagcgggctg ggcgcggccg
     3241 gcgactacta cgacgtgctg cgaccgcacg gcgtgtgggc cgtgatcagc ccgttcaact
     3301 tcccgatggc gctgtcgggc ggaccgtcca gcggggcgtt ggtggccggc aacacggtcg
     3361 tgctgaagcc ctcgaaccag ggcgcgctcc tgggctacaa gctctacgag tgctatcgcg
     3421 acggcggcgt gccggccggc gccttccacc tggtgacggg ccgtggcgcg gtggccggcg
     3481 accacctgtg gcaccacccc gacgtgaacg gcatcacctt cacggggtcc tacccggtgg
     3541 gcatggacat ctacaagcac ttctccaccg acgtgcccaa gccggtgatc tgcgagatgg
     3601 gcggcaagaa ccccacgatc gtcaccaaga acgcggacct ggacaaggcc accgaaggtg
     3661 tgttgcgcag cgccttcggg ttcggcgggc agaagtgctc ggcctgcagc cgggtctacg
     3721 tggagcgcga ggtctatgac gacttcgtca cccagctcaa gaccaagacc gagaagatca
     3781 aggtcggcaa cccgatcgag cgcgacgtct acatgggtcc gatcatcaac gagcccgcga
     3841 tggagaccta c
//
LOCUS       DATDBZ010000555         3204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2234328, whole genome shotgun sequence.
ACCESSION   DATDBZ010000555 DATDBZ010000000
VERSION     DATDBZ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234328"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VIX62_12775"
     CDS             <1..296
                     /locus_tag="VIX62_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY5861150.1"
                     /translation="RREAPSRDTWRAVGSDRYPAGMATYEYICLGCERPFEQRRSMSA
                     VDTALECPSCGSDRVRRRFSIFAAGAISTTGSTGPAPSAGGGCCGGGGCACGG"
     gene            363..779
                     /locus_tag="VIX62_12780"
     CDS             363..779
                     /locus_tag="VIX62_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861151.1"
                     /translation="MDERALDFWIGDWDCRWHDGYGVNSVARELDDAVVVERFEALGD
                     EPFRGMSVSVFDEMAGWRQTWVDANGSYWHLVGSALDDGSVVFGTPDPVDADRAFKRM
                     VFSGIEYDGFDWRWESSPDAERWQERWTIRYTRRRS"
     gene            complement(795..1007)
                     /locus_tag="VIX62_12785"
     CDS             complement(795..1007)
                     /locus_tag="VIX62_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HEY5861152.1"
                     /translation="MPLRAVDGDGPLGRDIPAEVLRFAARPAEQRQLLLHVHDLLGGI
                     AYGTVVLVLQDGKVIQVETSEKIRLK"
     gene            complement(1010..2122)
                     /locus_tag="VIX62_12790"
     CDS             complement(1010..2122)
                     /locus_tag="VIX62_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5861153.1"
                     /translation="MSTPIVVEQLTKRFGSFEAVRDVSFTAPGGAITALLGPSGSGKS
                     TVLRMIAGLEAPNEGRIWLGDVELTPKSVQERRVGFVFQHYALFRHMTVADNVAFGLA
                     VRKRSKSQRRERVDELLELVQLTHFAKRYPDQLSGGQRQRVALARALAPRPEVLLLDE
                     PFGALDAKVRQDLRQWLDELHRELGVTSLLVTHDQEEALELANQIVVMHEGRVQQVGA
                     PNEIYDDPKTPFVAGFVGSANVLHGVVEGGHVHLGAFRVAGAEHLAEGSAATAFIRPH
                     DVRVRPNGSNGHGAEGPTAIGRVERISSLGWLARLTIRLDGEGNHTLVAHVPQSDLAG
                     ATEGDLVSVDLRNPKAFDRVEAEAVSQGASEVATAE"
     gene            complement(2119..2979)
                     /locus_tag="VIX62_12795"
     CDS             complement(2119..2979)
                     /locus_tag="VIX62_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HEY5861154.1"
                     /translation="MRTPSDAPSVSLPRSGRRRLVRSGIVTACVLYVGVLLLLPLVGI
                     VYTALEPGWSKVVETFTRPDVQHAFFLTGVITVITVVVTTIFGVITAWVLVRQRFVGK
                     SLLNALVDLPFALSPVTVGLAAVILFGFGGWLEPFFDARGIQVIFAVPSMVLVTIFIC
                     IPFTIREVVPVLEEIGKDEEDAARTLGASILQTWRKVTLPNIRWGILYGVALTIARSI
                     GEIGAVLIVSGLIKGKTETATLFIFTAIEERQEAEAYIVALTLAAVSIVLLVGIETAR
                     HRIEGRKVRT"
     gene            complement(2976..>3204)
                     /locus_tag="VIX62_12800"
     CDS             complement(2976..>3204)
                     /locus_tag="VIX62_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY5861155.1"
                     /translation="GTLLTFARCLGEFGSVVLVAGNRAGETLTAPVFIFQLANQFRPI
                     EAAAVATLLFSISFVLVLVTARLLKRKEDDA"
BASE COUNT          508 a         1107 c         1122 g          467 t
ORIGIN      
        1 gtaggcgtga ggctccttcg cgcgacacct ggcgcgccgt cggttcggac cgctatcctg
       61 cgggcatggc cacctacgag tacatctgcc tcggctgcga gcggcccttc gagcagcggc
      121 gctcgatgag cgcggtcgac acggccctcg agtgcccgtc gtgcggcagc gaccgggtgc
      181 gccggcgctt ctcgatcttc gccgcaggcg ccatctcgac cacgggttcc accggcccgg
      241 ccccgtccgc cgggggcgga tgctgcggcg gtggcggctg cgcctgcggc ggctgagccg
      301 gccgcgaagt agcggcaatc ctgcggaaac gtcggggccg agcccgacct aggctctcgc
      361 gcatggacga gcgcgcgctc gacttctgga tcggcgactg ggactgccgc tggcacgacg
      421 gctacggcgt gaactccgtc gcccgcgagc tcgacgatgc ggtggtggtc gagcggttcg
      481 aggcgctcgg cgacgagccg ttccggggca tgagcgtctc ggtgttcgac gagatggccg
      541 ggtggcggca gacctgggtc gacgccaacg gcagctactg gcacctcgtg gggtcggcgc
      601 tcgacgacgg cagcgtggtg ttcgggacgc ccgaccccgt ggacgccgac cgggcgttca
      661 agcggatggt gttctccggt atcgaatacg acgggttcga ctggcggtgg gaatcgtcgc
      721 ccgacgccga gcggtggcag gaacgctgga cgatccggta cacgcgccgc cgctcatgag
      781 ggccaacggt ctgctcactt gagccggatc ttctccgagg tctccacctg gatcaccttg
      841 ccgtcctgca gcacgagcac caccgtgccg taggcgatgc cgcccagcag atcgtgcacg
      901 tgcagcagga gctgccgctg ctcggcaggc ctggctgcga accgaagcac ctctgccggg
      961 atgtcccggc cgaggggccc gtcgccgtcc acggctcgaa gaggcaccgt cactccgcgg
     1021 ttgcgacctc ggaggcgccc tgcgacacgg cctcggcctc gacacgatcg aacgccttcg
     1081 gattgcgcag atccacgctc accaggtcgc cctcggtggc accggccagg tcggactgcg
     1141 gcacgtgcgc gacgagggtg tggttgccct cgccgtcgag ccggatcgtg agccgggcga
     1201 gccacccgag cgaggagatt cgctccaccc tgccgatcgc cgtcggcccc tccgcgccgt
     1261 ggccgttcga gccgttcggc cgcacccgca cgtcgtgcgg tcggatgaag gccgtggcgg
     1321 ccgacccttc cgcgaggtgc tccgccccgg cgacccggaa ggcgccgaga tgcacgtggc
     1381 caccctcgac gaccccgtgc agcacgttgg ccgaacccac gaaccccgcc acgaaggggg
     1441 tcttcgggtc gtcgtagatc tcgttcggcg cgccgacctg ctgcacacgg ccctcgtgca
     1501 tcacgacgat ctggttcgcc agctccaacg cttcctcctg gtcgtgggtc accagcaggc
     1561 tggtcacgcc cagttcacga tgcagctcgt cgagccactg gcgcaggtcc tggcgcacct
     1621 tcgcgtcgag ggcgccgaac ggctcgtcga gcaacagcac ctcgggccgt ggggccagcg
     1681 ctcgggccag cgccacccgc tggcgctgcc cgcccgagag ctggtcgggg tagcgcttgg
     1741 cgaagtgcgt gagctgcacg agctcgagca actcgtcgac acgctccctg cgttggctct
     1801 tcgaccgctt ccgcacggcg aggccgaatg cgacgttgtc ggccaccgtc atgtgacgga
     1861 acagggcgta gtgctggaac acgaacccga cacggcgctc ctgcacgctc ttcggcgtga
     1921 gctcgacgtc accgagccag atgcggccct cgttgggcgc ctcgaggccc gcgatcatgc
     1981 gcaggacggt gctcttgccc gagcccgagg gaccgagcag cgcggtgatc gcgccgcccg
     2041 gagccgtgaa cgacacgtcg cgcacggcct cgaacgagcc gaaccgtttg gtgagctgct
     2101 ccaccacgat cggcgtgctc atgtgcgtac cttccttcct tcgatgcgat gtcgcgccgt
     2161 ctcgatgccg acgagcagca cgatggacac ggcggccagc gtgagggcga cgatgtaggc
     2221 ctcggcctcc tgacgctcct cgatggcggt gaagatgaac agggtggcgg tctcggtctt
     2281 gcccttgatg agcccgctca cgatcagcac ggcgccgatc tcgccgatgg aacgagcgat
     2341 cgtgagggcg acgccgtaga ggatgcccca gcggatgttc ggcagggtca ccttgcgcca
     2401 ggtctgcagg atcgaggcgc cgagcgtgcg agccgcgtcc tcctcgtcct tgccgatctc
     2461 ctcgagcacc ggcacgacct cgcggatcgt gaacgggatg cagatgaaga tcgtcacgag
     2521 caccatcgag ggcaccgcga agatcacctg gatgccgcgg gcgtcgaaga agggctcgag
     2581 ccaccccccg aagccgaaca ggatgaccgc ggcgagcccg acggtcacgg gcgacagtgc
     2641 gaacggcagg tcgacgagcg cgttcagcag cgacttgccg acgaaccgct gccgcacgag
     2701 cacccaggcg gtgatcacgc cgaagatcgt ggtcaccacg accgtgatca ccgtgatgac
     2761 gccggtcagg aagaaggcgt gctgcacgtc gggccgcgtg aacgtctcga ccactttcga
     2821 ccagcccggt tccagcgcgg tgtagacgat gcccacgagg ggcaggagca gcaggacgcc
     2881 gacgtacagg acgcacgccg tcacgatccc gctgcgcacg aggcgccggc gaccgctgcg
     2941 cggaagggag acggaggggg catcggaggg ggtcctcatg cgtcgtcctc cttccgcttc
     3001 aagaggcgag cggtcaccag cacgagcacg aaggagatgc tgaacagcag cgtcgcgacc
     3061 gccgccgcct cgatcgggcg gaactggttc gcgagctgga agatgaagac gggcgcggtg
     3121 agcgtctcac ccgctcggtt gccggcaacg aggacgacgc tgccgaactc accgaggcac
     3181 cgggcgaacg tcaacagcgt gcca
//
LOCUS       DATDBZ010000556        10591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1201154, whole genome shotgun sequence.
ACCESSION   DATDBZ010000556 DATDBZ010000000
VERSION     DATDBZ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201154"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VIX62_12805"
     CDS             complement(<1..463)
                     /locus_tag="VIX62_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY5861156.1"
                     /translation="MRTLTQRELNRALLARQGLLERFDRSLPRCIESVGGLQTQYAPS
                     GYIGTWSRLEGFERGALTRALERRTVVQATLMRSTIHMVSRRDYWPIATAVREHRRRW
                     WLQATRHAMSERDLETWAGRIRAALADGPQRRSVLVKELSMDSTTWNGATGW"
     gene            508..1782
                     /locus_tag="VIX62_12810"
     CDS             508..1782
                     /locus_tag="VIX62_12810"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY5861157.1"
                     /translation="MPASIVLGTQWGDEGKGKAVDYLADGMDLVVRYQGGNNAGHTVI
                     AEGRLLKLQLIPSGILYPHITSVIADGVVVDPRHLLKEMDAVRETGVDVSRLVVSGNA
                     HMIMPYHLELEKVTERFLGKNALGTTKRGIGPAYGDKAARIGLRIQDLFDEKIFRQKL
                     EVVLREKNLILTKIYNRLPLDAERIIDEYMSLAERIQPMVVDTSSLLYHALEDGKHVM
                     FEGAQGVLLDLDHGTYPFVTSSNPVAGSAFASAGIGPGWAERIVGIMKAYITRVGAGP
                     FPTELLDEVGERLGTRGKEFGTVTGRQRRCGWFDACLGRYAARLNGLTELLVTKLDVL
                     SGLETVKICTGYRAYGQSFDDMPPHQSVFHDASPIYEELEGWQEEIDECQSFEDLPKQ
                     AQAYVRRMEELVGVPVSVVSVGPAREQSLPVG"
     gene            1787..3064
                     /gene="purD"
                     /locus_tag="VIX62_12815"
     CDS             1787..3064
                     /gene="purD"
                     /locus_tag="VIX62_12815"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015539257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY5861158.1"
                     /translation="MKVLVVGGGGREHALTWGLARSPLVTELHVAPGNAGIAQLATCH
                     DVAADDVEQMVKLVDQLGPDLVVVGPEAPLVAGLADRVRAMGVPAFGPGADAARLEGS
                     KAWSKQVMEAGNIPTAGAGTFTEVGPAAAFVRELGGRAVVKADGLAAGKGVTVATEPS
                     AAEQAIRDCLEGGAFGEAGETVVVEELLEGPEVSSFALVAGGQVVHLGLSQDFKRIGD
                     GDTGPNTGGMGAYSPLPFVDRATEGSIEDIAERTVAALGSMGIGYSGLLYTGVMLTAD
                     GPKVLEYNCRFGDPETEVIVPRLAGDLANLLMAVATDRPGDAEVVMSADAAVTVVVAS
                     GGYPGVYETGVPIDGLDAAAEVPGAVVFHAGTAQADGRVVTAGGRVLSVTGTGASIGE
                     ARDHAYAAVARVSFEGMTTRTDIAQAATYREGT"
     gene            3061..3537
                     /locus_tag="VIX62_12820"
     CDS             3061..3537
                     /locus_tag="VIX62_12820"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEY5861159.1"
                     /translation="MTDETTRVGVLAASPSELSTMRQAGLILEKFGVGHEIRIMSSSR
                     NPDLVDEYARTAFDRGLKVIICSTGLSGHLAAAVAARTVLPVIGVPIATAPQMVGNEA
                     LAITAQMPTGVPVATVGVESSVNAAVLAAQIIGVSDPEVQAALWRFKDDLAEGLKL"
     gene            3534..4853
                     /gene="purB"
                     /locus_tag="VIX62_12825"
     CDS             3534..4853
                     /gene="purB"
                     /locus_tag="VIX62_12825"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY5861160.1"
                     /translation="MIPRYSLPEMAAVWSDERRLASWLEIELLAVEAWAELGTIPADD
                     AATCRARASFTVEAVSEREQVTRHDIAAFVDVVADSIGPEGRWIHFGLTSSDVLDTGF
                     ALQLRDAADILLAKLEHLLAVVKQQALAYRDTPIMGRSHGVHAEPTSFGHKLAVWAFE
                     LDRDRERLRRARETVSVGAISGVVGSYAAVDPRIEELVCARLGLRAEDASTQVIQRDR
                     HAEFVTTLAIVASTLDKVATEIRHLARTEVREVQEPFAAGQKGSSAMPHKRNPVVCER
                     ISGLARVVRGHAQVSLENVALWHERDISHSSAERIILPDSTGLLDFMLTDAAWVLDGL
                     VVFPDAMLDNVMSHGGIAFSQSALLALVEAGWPREDAYRAVQAAAASAWDEGASFREA
                     LEADPLVREALGDDGLEALFDPQRFLRNLGGVFDALEKLPVSEGAPS"
     gene            4850..5782
                     /locus_tag="VIX62_12830"
     CDS             4850..5782
                     /locus_tag="VIX62_12830"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY5861161.1"
                     /translation="MTTAGGLRVRGKVRDVYDAGPGALLLVATDRLSAFDVVLPEPVP
                     DKGRVLTACSLFWFERTRDLVPNHVRTADPSAFPPPFTSDPELAGRSIVVERASVIPV
                     ECVARGFLSGSGWAQYRDGGSVCGVALPAGLRESDRLPEPIFTPTTKAETGHDLPLTF
                     EQMSDLVGRGLAERLRELTVGIYDRIAQVALERGIIVADTKVEFGFAGGELLLIDEVG
                     TPDSSRFWPLDGYEAGRAQPSFDKQFVRDWLDASGWDREPPPPNLPADVIDKTAAKYR
                     EAYERVTGESWPAYLARMSPGNVDRNGEREGEQA"
     gene            5779..6045
                     /gene="purS"
                     /locus_tag="VIX62_12835"
     CDS             5779..6045
                     /gene="purS"
                     /locus_tag="VIX62_12835"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEY5861162.1"
                     /translation="MRCTFEVLVTLKAGLADPAGKAVEASLPAMGWTNVSGVHVGRHI
                     QLDVEAPNEDDARAQVAEIAKQLLSNPVIEDFRILETQGSTERA"
     gene            6042..6758
                     /gene="purQ"
                     /locus_tag="VIX62_12840"
     CDS             6042..6758
                     /gene="purQ"
                     /locus_tag="VIX62_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY5861163.1"
                     /translation="MTVEAPRVGVITFPGSLDDRDALRAVDLMGGVGVPLWHADHDLH
                     EVDAVVLPGGFSYGDYLRCGAIAGHAPILDEVKAFADRGGPVLGICNGFQILCESGLL
                     PGALIRNRSLRFVCRDVYLRVETSATPVTAEMTVGEVVEIPVKHGEGQFVARAEDLRR
                     IEDDGLVVFRYCDADGATGDEHNPNGSTDHIAGVRNAAGNVVGLMPHPEHAVDPDVGP
                     TGGQPLFASLFARALAGAGA"
     gene            6755..8959
                     /gene="purL"
                     /locus_tag="VIX62_12845"
     CDS             6755..8959
                     /gene="purL"
                     /locus_tag="VIX62_12845"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY5861164.1"
                     /translation="MSVTQEPLHRTLGLKDDELERIRDTLGRDPRPAELAMYGAMWSE
                     HCSYKSSKIHLKTLPTEGPAVLVGPGQDAGAVDVGDGWAAVFKMESHSHPSAIEPYQG
                     AATGVGGIVRDIISMGARPVALLDPLMFGPLTDARNRWLLEGVVAGIGGYGNCIGVPT
                     VGGEIRFAEPHSANPSVDVMCVGLARADELVTSSSLEAHAGSLMVLYGATTGRDGIGG
                     VSVLASATLDDSSQDARPSVQIGDPFAEKLLIEASLELIQQGLLEGLQDLGGAGITCA
                     VSESAARANMGAVLDLDAVPLREAGMEAFEILTSESQERMLAIVHPSKLEAVTEVCER
                     WGLRTAVIAELVEGGTLTVTHGGEVVAEMPATSLTDDGPEYDRPKEAPVTPVEDDPTF
                     APFEGDLREAFLTVVTSPNVASKAWIWEQYDSIVQGQTVAGSGSDAAIVRVPGTLKGL
                     ALSSDGKGRFGALDPYLGTMHAVAEAARNVAVSGARPLAITNCMNFGNPERPVVMWQF
                     AESIRGMRDACEALATPVTGGNVSFYNETGDSAIWPTPVIGMLGLLEDHRLRIPTAFP
                     RAGLAIYQLGETFAELGGSEFAAAVFGIVAGHPPALDLHRERALHDLLYEGARNDVLA
                     SAHDCSDGGLAIALAEAAIGDGHGFAVTVGSDLPSHVELFSESASRVVVSVAPEHEEQ
                     LRELGTAHGVPMARLGETGGPRAVFAGLFEIDVAELREAYETAIPRLLGETG"
     gene            8965..9696
                     /locus_tag="VIX62_12850"
     CDS             8965..9696
                     /locus_tag="VIX62_12850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5861165.1"
                     /translation="MVIEAVTFDFWDTLVAMDGSSVTMRDRQIDGFAEALLRHGHTRE
                     RDELVEVFAENWTRFEAQWEANAGQYTPADTTDFIVERLGVPVTPAMRADLIDAFRIV
                     GERADLEPAPGIEECLSTLRGAGVKLAIVCDVGLTPSPTLRDRLTGFGLLRWFDGWAF
                     SDETGWFKPAPEAFLPALEALGIADPTVAAHVGDNRRTDVAGALALGMTAVRYTGLRE
                     ATAAGPEADHVVDDHRKIPAVLGLA"
     gene            complement(9701..>10591)
                     /locus_tag="VIX62_12855"
     CDS             complement(9701..>10591)
                     /locus_tag="VIX62_12855"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5861166.1"
                     /translation="DRAHAIPNTVETLFATASATKGFTALTVMSLVEDGTLELTTTVR
                     SVLGGDLPLIADDVTLEHLMAHRSGIGDYLDEDEVESVTDYVMPVPVHRLATTEAFLE
                     VLDGHETVFPAGERFAYNNGGFVVLALIAERASGVPFHDLVQQRVCEPAGMVDTAFLR
                     SDELPGRAALNYLEKDGSRTNVLHLPVRGTGDGGIYSTADDLHAFWGALFAGRIVPME
                     RVAEMVRPRSDWPEEERRYGLGFHLHATSDVVWLEGYDAGVSFRSVVDPTSLTTHTVI
                     SNWSDGAWDLTWDIDEILGL"
BASE COUNT         1583 a         3532 c         3871 g         1605 t
ORIGIN      
        1 gccatcccgt ggcaccgttc caggtcgtgg agtccatgct tagctccttc accagcacgg
       61 atcggcgttg ggggccgtcg gcgagcgcag cccggatccg gccggcccag gtttcgaggt
      121 cgcgctcgct catggcatga cgtgtggcct gcagccacca ccggcgccga tgctcccgga
      181 ccgcggtggc gatcggccag tagtcgcgac gggagaccat gtggatcgtg ctgcgcatca
      241 gggtggcctg caccaccgtc cttcgctcca gcgcccgcgt gagcgccccg cgctcgaacc
      301 cctccaggcg cgaccacgtc ccgatgtagc cgctgggcgc gtactgggtc tggaggccac
      361 cgacgctctc gatgcaccgg ggcagcgagc ggtcgaagcg ctccaggagc ccttgtcgag
      421 cgaggagcgc ccggttcagc tcgcgttggg tgagggttcg catcgtcggg agtggagggc
      481 ggtgcccgcc ggtagactac gggcctcatg cctgcctcca tcgtgctcgg gacccagtgg
      541 ggagacgagg gcaagggcaa ggcggtcgac tacctcgcgg acggcatgga tctcgtcgtc
      601 cgctatcagg gcggcaacaa cgccggtcac acggtgatcg ccgagggtcg gctgttgaag
      661 ctccagctga tcccctcggg catcctctat ccgcacatca cgtcggtgat cgccgacggt
      721 gtcgtggtgg atccgcgcca cctgctgaag gagatggacg ccgtccgcga gaccggcgtc
      781 gacgtctccc ggctggtcgt gagcggcaac gcgcacatga tcatgcccta ccacctggag
      841 ttggagaagg tcaccgagcg gttcctcggc aagaacgccc tcggcaccac caaacgcggc
      901 atcggtcccg cctacggtga caaggccgcc cggatcgggc tgcgcatcca ggacctgttc
      961 gacgagaaga tcttccggca gaagctcgag gtggtcctgc gcgagaagaa cctgatcctg
     1021 acgaagatct acaaccggct gccgctcgat gccgagcgca tcatcgacga gtacatgtcc
     1081 ctggccgagc gcatccagcc catggtggtc gacacgtcgt cgttgctcta ccacgcgctc
     1141 gaggacggca agcatgtgat gttcgaaggc gcgcagggtg tgttgttgga cctcgaccac
     1201 ggcacgtacc cgttcgtcac ctcgtcgaac ccggtggccg gcagcgcctt cgccagcgcc
     1261 ggcatcggac cgggctgggc cgaacgtatc gtcggcatca tgaaggcgta catcacgcgt
     1321 gtcggcgcgg gtccgttccc cacggagctg ctcgacgagg tgggcgagcg tctcggcacc
     1381 aggggcaagg agttcggcac tgtcaccggc cgccagcggc gctgcggatg gttcgacgcg
     1441 tgcctgggcc ggtacgcggc gcgcctcaac gggctcaccg agctgctggt cacgaagctg
     1501 gatgtgttga gcggcctcga gaccgtgaag atctgcacgg gctatcgcgc ctacggccag
     1561 agcttcgacg acatgccgcc ccaccagtcg gtcttccacg acgccagccc gatctacgaa
     1621 gagctcgagg gctggcagga agagatcgac gaatgccagt cgttcgagga cctgcccaag
     1681 caggcccagg cctacgtgcg ccgcatggag gagctggtcg gcgtcccggt cagcgtggtc
     1741 agcgtgggcc ccgcccgcga acagagcctg cccgtcgggt gagcacgtga aggtcctcgt
     1801 cgtcggtgga gggggccggg agcacgccct gacgtggggg ctggcgcgga gcccgctcgt
     1861 caccgagctg cacgtcgctc ccgggaacgc cggcatcgcg cagctcgcca cctgccacga
     1921 cgtggcggcc gacgacgtcg agcagatggt caagctggtt gaccaacttg gcccggatct
     1981 cgtcgtggtc ggtcctgagg ctcccctggt ggccgggttg gccgaccggg tccgcgcgat
     2041 gggtgtcccg gccttcggac caggtgccga cgccgcccgc ctggaaggct ccaaggcgtg
     2101 gtcgaagcag gtgatggaag ccggcaacat ccccacggcg ggtgcgggaa cgttcaccga
     2161 ggtcggtccc gcggccgcgt tcgtccgcga gctcgggggc cgagcggtcg tgaaggccga
     2221 cggactcgcg gccggcaagg gcgtcacggt ggccacggaa ccctcggccg ccgagcaggc
     2281 gatccgtgat tgtctcgagg gcggcgcctt cggcgaggct ggcgagaccg tcgtggtgga
     2341 ggagttgctc gaaggaccgg aggtgagctc gttcgcgctg gtggccggcg gacaggtcgt
     2401 gcacctcggg ctctcacagg acttcaagcg gatcggcgac ggcgacaccg ggcccaacac
     2461 cggcggcatg ggcgcctact cgccgctgcc gttcgtcgat cgggcgaccg aaggctcgat
     2521 cgaggacatc gccgaacgga ccgtggcggc gctgggatcg atggggatcg ggtactcggg
     2581 actcctctac accggcgtga tgctcaccgc cgacgggccc aaggtgctcg agtacaactg
     2641 ccggttcggc gatcccgaga ccgaggtcat cgtgccaagg ctcgccggcg acctcgcgaa
     2701 cctcctgatg gcggtcgcca ccgaccgccc gggtgacgcc gaggtggtca tgagcgccga
     2761 cgccgcggtc accgtcgtcg tggccagcgg gggctacccg ggggtctatg agaccggcgt
     2821 gcccatcgac gggttggacg ccgcggccga ggttcccggc gccgtggtct tccatgccgg
     2881 gaccgcgcag gccgacggta gagtggtgac ggcgggggga cgcgtgctgt ccgtcaccgg
     2941 aaccggcgcc tcgatcggtg aggcccgcga ccacgcgtat gcggcggtgg cgcgcgtctc
     3001 gttcgagggc atgaccacac gaacagacat cgcccaggcg gcgacgtaca gggagggaac
     3061 gtgaccgacg aaaccacgcg cgtgggggtg ctcgcggcgt cgccgtccga gctcagcacg
     3121 atgcggcagg ctgggctgat cttggagaag ttcggggtcg gtcacgagat ccggatcatg
     3181 tcctcgtccc gcaaccccga cctggtggac gagtacgccc ggaccgcctt cgatcgcggt
     3241 ctcaaggtca tcatctgttc caccgggctg tcgggccacc tggccgcggc ggtcgcggct
     3301 cggaccgtgc tcccggtgat cggcgtgccg atcgccacgg cgccgcagat ggtgggcaac
     3361 gaagcgctgg cgatcaccgc ccagatgccc accggggtcc ccgtggccac ggtcggcgtc
     3421 gagtcctcgg tcaatgcggc ggtgttggcg gcccagatca tcggggtgtc cgatcccgag
     3481 gtgcaggcag cgttgtggcg gttcaaggac gacctcgcgg agggcctcaa gctgtgatcc
     3541 cgcggtacag cctgccggag atggccgcgg tctggtccga cgaacggcgg ctggcatcct
     3601 ggctcgagat cgagctcctc gccgtcgagg cgtgggccga gctgggcacg atccccgccg
     3661 acgacgcggc cacctgccgg gcgcgcgcct cgttcaccgt ggaggccgtg tcggaacggg
     3721 agcaggtcac ccggcacgac atcgcggcgt tcgtggacgt ggtggcggac tcgatcggcc
     3781 ccgagggacg ctggatccac ttcggcctca cgtcctcgga cgtgctcgac accgggttcg
     3841 cgttgcagct ccgcgacgcc gccgacatcc tgctggctaa gctcgagcat ctgctggcgg
     3901 tcgtgaagca gcaggcgctg gcctaccgcg acacgccgat catgggccgc tcgcacggcg
     3961 tgcatgccga gcccacctcg ttcgggcaca agctggccgt ctgggcgttc gagctggatc
     4021 gagaccgcga acggctgcgc cgcgcccgcg agaccgtgag cgtgggcgcg atcagcgggg
     4081 tcgtgggctc ctacgccgcc gtcgatcctc ggatcgagga gctggtgtgc gcccggctgg
     4141 gccttcgcgc cgaggacgcc agcacccagg tgatccagcg cgaccgtcac gccgagttcg
     4201 tcacgacgct ggcgatcgtg gcctccaccc tggacaaggt ggcgaccgag atccggcacc
     4261 tggcgcgcac cgaggtccgt gaggtccagg aaccgttcgc ggccgggcag aagggctcga
     4321 gcgccatgcc gcacaagcgg aacccggtgg tgtgcgagcg catcagcggg ctggcgcgcg
     4381 tcgtgcgggg gcacgcacaa gtgtcgctgg agaacgtggc gctctggcac gaacgcgaca
     4441 tctcgcactc gtcggccgaa cgcatcatcc tgcccgattc cacggggctg ctggatttca
     4501 tgctcaccga cgcggcgtgg gtcctggacg gcctggtcgt gttccccgac gcgatgctcg
     4561 acaacgtgat gagccacggg gggatcgcgt tcagccagtc ggcgctgctg gccctggtcg
     4621 aagccggatg gccgcgcgag gacgcctacc gtgccgtgca ggccgccgcc gcctctgcct
     4681 gggacgaggg cgccagtttc cgtgaggcgc tggaggccga cccgttggtt cgcgaggcgt
     4741 tgggcgacga cgggctggag gcgctgttcg atccgcagcg gttcctgcgc aacctcggcg
     4801 gcgtgttcga tgccctggag aagctgccgg tctcggaggg agcgccctcg tgaccaccgc
     4861 cggcggcctg cgcgtccgag gcaaggtgcg ggatgtctac gacgccggac cgggcgccct
     4921 gctcctggtc gccaccgatc gcctcagcgc cttcgacgtc gtcctgcccg agcccgtccc
     4981 cgacaaggga cgggtgctga ccgcctgctc gctgttctgg ttcgagcgca cgagggacct
     5041 cgtaccgaac cacgttcgga ccgccgaccc gtcggcgttc ccgccgccgt tcacgagcga
     5101 tcccgaactc gcgggccgtt cgatcgtggt ggagcgggcc tccgtgatcc ccgtggaatg
     5161 cgtcgcgcgc ggcttcctca gtgggtcggg gtgggcgcag tatcgcgacg gcggctcggt
     5221 gtgcggcgtg gcgctgcccg ccggcctgcg ggaatccgac cggctgcccg agccgatctt
     5281 cacgcccacc acgaaggccg agacgggcca cgacctgccg ttgaccttcg agcagatgtc
     5341 ggatctagtg gggcggggcc tcgccgaacg cctgcgcgag ctcaccgtcg gcatctacga
     5401 tcgcatcgcc caggtggccc tcgaacgcgg catcatcgtg gccgacacga aggtcgagtt
     5461 cgggttcgcc ggcggcgagt tgctcctgat cgatgaggtg ggcacgcccg attcgtcccg
     5521 cttctggccg ctcgacggct acgaggcggg gcgcgcgcag cccagcttcg acaagcagtt
     5581 cgtgcgtgac tggctggatg cgagtggctg ggaccgcgag ccgcccccgc cgaacctgcc
     5641 tgcggacgtg atcgacaaga ccgccgcgaa gtaccgcgag gcctacgaac gcgtcacggg
     5701 ggagtcgtgg ccggcctacc tggcgcggat gagcccgggg aacgtggatc ggaacgggga
     5761 gcgggaaggg gagcaggcat gaggtgcacg ttcgaggtgc tggtgacgct gaaggccggc
     5821 ctcgccgatc cggccggcaa ggcggtggaa gcctcgctgc ccgccatggg gtggacgaac
     5881 gtgtcggggg tccacgtcgg acggcacatc cagctcgacg tcgaggcccc gaacgaagac
     5941 gacgctcgcg cgcaggtcgc cgagatcgcg aagcagttgc tgtccaatcc ggtgatcgaa
     6001 gacttccgca tcctggagac ccaggggagc accgaacggg catgacggtc gaggcccccc
     6061 gcgtcggcgt gatcacgttc cccgggtcgc tggacgatcg cgacgcgctg cgcgcggtgg
     6121 acctgatggg cggggtgggc gtgccgttgt ggcacgccga ccacgatctg cacgaggtcg
     6181 acgcggtggt gttgccgggt gggttcagct acggcgacta tctgcgatgc ggcgccatcg
     6241 cgggccatgc gccgatcctg gacgaggtca aggcgttcgc cgaccgtggc gggccggtgc
     6301 tcggcatctg caacggcttc cagatcctct gcgaatcggg gctgttgccc ggcgcgctga
     6361 tccgcaaccg gtcgttgcgc ttcgtctgcc gcgacgtgta cctgcgggtg gagacctccg
     6421 ccacgccggt cacggccgag atgacggtgg gcgaggtcgt cgagatcccc gtgaagcacg
     6481 gcgaaggaca gttcgtggcg cgcgccgagg atctgcgtcg gatcgaggac gacggcttgg
     6541 tggtgttccg gtactgcgac gccgacgggg ccaccggcga cgagcacaac cccaacggat
     6601 ccaccgatca catcgccggc gtgcgcaacg ccgcgggcaa cgtggtgggg ctcatgccac
     6661 atccggagca cgcggtcgat cccgacgtcg gacccacggg tggacagccg ctcttcgcgt
     6721 cgctgttcgc ccgggcgctg gcgggagcag gcgcgtgagc gtgacgcagg agccgctcca
     6781 ccgcacgctc ggcttgaagg acgacgagct cgaacggatc cgcgacacgc tgggccgcga
     6841 tccccggccc gccgagctcg cgatgtacgg tgcgatgtgg tccgagcact gctcgtacaa
     6901 gagctcgaag atccacctga agacgctccc caccgaaggt cccgccgtgc tcgtcggccc
     6961 cggccaggac gccggagcgg tcgacgtggg cgatggctgg gccgccgtgt tcaagatgga
     7021 atcgcactcc caccccagcg cgatcgagcc ctatcagggt gcggccaccg gcgtgggggg
     7081 catcgtgcgc gacatcatct cgatgggcgc ccgccccgtg gcgttgctgg atccgctgat
     7141 gttcggcccg ctcaccgatg ctcggaaccg ctggctgctg gagggcgtgg tggccggcat
     7201 cggcggctac ggcaactgca tcggcgtgcc caccgtgggg ggcgagatcc ggttcgccga
     7261 acctcattca gcgaacccga gcgtcgacgt gatgtgcgtc ggcctggccc gagccgacga
     7321 actggtgacc tcctcgtcgc tggaggcgca cgcggggtcg ctgatggtgc tgtacggcgc
     7381 caccaccggc cgcgacggca tcggcggcgt gagtgtgctc gccagcgcca cgctggacga
     7441 ttcgagccag gacgccagac cctcggtgca gatcggcgac ccgttcgccg agaagctgtt
     7501 gatcgaagct tcgctcgagc tgatccagca gggcctgctc gaaggactgc aggacctggg
     7561 cggcgccggg atcacctgcg cggtgagcga gtcggcggcg cgggcgaaca tgggcgccgt
     7621 gctcgacctc gacgccgtcc cgttgcgcga ggcgggcatg gaggcgttcg agatcctcac
     7681 cagcgaatcg caggagcgca tgctcgcgat cgtgcacccc tcgaagctcg aggccgtcac
     7741 cgaggtgtgt gagcgatggg ggctgcgcac ggcggtgatc gccgaactcg tcgagggcgg
     7801 cacgctcacc gtgacgcacg ggggtgaggt cgtcgccgag atgcctgcca cgtcgctgac
     7861 cgacgacggt cccgaatacg accggccgaa ggaagctcct gtgacaccgg tcgaagacga
     7921 tcccacgttc gcgccgttcg agggcgacct gcgcgaggcg ttcctcaccg tggtgacctc
     7981 gcccaacgtg gcgagcaagg cgtggatctg ggagcagtac gactcgatcg tccagggcca
     8041 gaccgtcgcc gggtcgggca gcgacgccgc catcgtgcgt gtgcccggga cgctgaaggg
     8101 gctcgcgctc agcagcgacg gcaagggccg gttcggggcg ctcgatccgt acctgggcac
     8161 gatgcacgcc gtggccgagg cggcacggaa cgtcgcggtg agcggcgcca ggcccctcgc
     8221 gatcacgaac tgcatgaact tcggcaaccc cgagcggccg gtcgtgatgt ggcagttcgc
     8281 cgagtcgatc cgcggcatgc gcgatgcctg tgaagcgctg gcgacccccg tcaccggtgg
     8341 caacgtcagc ttctacaacg agacgggcga ctcggcgatc tggcccacgc ccgtgatcgg
     8401 catgctgggg ttgctcgagg accaccggct gcggatcccc accgcgttcc cgcgagcggg
     8461 gctggcgatc taccaactgg gggagacctt cgccgaactg ggtggctcgg agttcgccgc
     8521 agcggtcttc ggcatcgtgg ccgggcatcc acccgcgctg gatctccacc gcgagcgagc
     8581 cctgcacgac ctgctgtacg aaggggcgcg gaacgatgtg ctcgccagcg cccacgactg
     8641 cagcgacggc ggactcgcga tcgcgctggc cgaagccgcg atcggtgatg gccacgggtt
     8701 cgcggtgacg gtcggctcgg acctgccgtc ccacgtggaa ctcttcagtg aatccgcctc
     8761 tcgcgtggtg gtgagcgtgg cgcccgagca cgaggagcag ctgcgggagc tcggcacggc
     8821 gcacggtgtg ccgatggcgc ggctcggcga gaccggaggg ccgcgggcgg tgttcgccgg
     8881 gctcttcgag atcgacgtgg ccgagttgcg ggaggcctac gagaccgcga tcccgcgcct
     8941 gctcggcgag accggctgac gccggtggtc atcgaggcgg ttaccttcga cttctgggac
     9001 acgctggtgg cgatggacgg ctcgtccgtg acgatgcgcg accgccagat cgacgggttc
     9061 gccgaggcgc tgctccggca cggtcacacc agggagcgtg acgagctcgt ggaggtgttc
     9121 gccgagaact ggacccggtt cgaggcgcag tgggaggcca acgccggtca gtacacgccc
     9181 gccgacacca ccgacttcat cgtggagcgg ctcggtgtcc cggtcacgcc ggcgatgagg
     9241 gccgacctga tcgacgcgtt ccggatcgta ggggagcggg ccgacctgga gccggcgccc
     9301 ggcatcgagg aatgcctgtc gaccttgcgg ggagccgggg tcaagctggc gatcgtgtgc
     9361 gacgtcgggc tcaccccttc gcccacgttg cgtgaccgtc tcacggggtt cgggctgctg
     9421 cgatggttcg acggatgggc cttcagcgac gagaccgggt ggttcaagcc ggctcccgag
     9481 gccttcctgc cggcgctcga ggcgctcggc atcgcggatc caacggtcgc ggcgcacgtc
     9541 ggggacaacc gtcgcaccga tgtggcgggc gcgctcgcgc tgggtatgac ggcggtgcgg
     9601 tacaccgggc tccgggaggc gacggccgcg ggtcccgagg ccgaccatgt cgtcgacgac
     9661 catcggaaga tcccggcggt gctcggcctc gcctgagtgg ctagagcccg aggatctcgt
     9721 cgatgtccca ggtgaggtcc cacgcaccat cggaccagtt ggagatcacg gtgtgggtgg
     9781 tgagggacgt ggggtcaacc acgctccgga acgacacgcc ggcgtcgtag ccctcgagcc
     9841 acacgacgtc gctcgtggca tgcaggtgga agccgagccc gtaccgccgc tcttcctccg
     9901 gccagtcgct ccgtggccgc accatctcgg ccacgcgctc catcgggacg atgcgcccgg
     9961 cgaacagtgc accccagaag gcgtggaggt cgtccgccgt cgagtagatg ccgccgtcgc
    10021 cggtgccgcg aaccggcaga tggaggacgt tcgtgcgcga cccgtccttc tcgagatagt
    10081 tcagggctgc cctgccaggg agctcatccg agcggaggaa cgcggtgtcc accatgcctg
    10141 cgggttcgca cacgcgttgt tggaccaggt cgtggaaggg caccccgctc gcgcgctcgg
    10201 cgatcaacgc cagcacgacg aagccgccat tgttgtacgc gaaccgttcc cccgcgggga
    10261 agacggtctc gtgcccgtcc aggacctcga ggaacgcctc ggtcgtggcg agccggtgca
    10321 cgggcaccgg catcacgtag tcggtgaccg actcgacctc gtcctcgtcg aggtagtcgc
    10381 cgatgcccga gcggtgcgcc atgaggtgct cgagcgtgac gtcgtcggcg atcaggggca
    10441 gatcaccccc gagcaccgag cggacggtcg tggtgagctc gagggtaccg tcctcgacca
    10501 ggctcatcac ggtgagtgcg gtgaacccct tcgtggcgct cgccgtcgcg aagagggtct
    10561 cgacggtgtt ggggatggcg tgcgcccggt c
//
LOCUS       DATDBZ010000557         3463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1410383, whole genome shotgun sequence.
ACCESSION   DATDBZ010000557 DATDBZ010000000
VERSION     DATDBZ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410383"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VIX62_12860"
     CDS             <1..590
                     /locus_tag="VIX62_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861167.1"
                     /translation="NTEQISIRSAIRAAEDVAAQHGVATDDLAFDRLMLQYEAGGTSE
                     GDGTMREPVVTETIVQFTQMIDGVPVLAPGRGQLSVGIDNDGRPTKIRNTVRPIAEVT
                     ERGRRTPPVPEAEAVASGGDDIEGMLGDAWQKRMRAFLVQDRMPRSFAEVPGTTEIGY
                     VMDGDEAALVVRREYEVDHGRGILKRHRIEVPLAE"
     gene            904..1503
                     /locus_tag="VIX62_12865"
     CDS             904..1503
                     /locus_tag="VIX62_12865"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY5861168.1"
                     /translation="MSFLALHDRPLQRAFVAGTCWPAASEKRALASLRSVLWRLHRLG
                     LTLVNASTDHVWLDVQVEVDYREATAVARVVLESSGDLDPEELKLLQLGETLLPDRFE
                     DWAVMERERLMQLRLHALERLSERLVAAGMYAEAVEAALAAVAAEPLRESAHRALIGV
                     YLAEGNTISAHRQYEVCRRLLDDELQVAPSRLMQELMSD"
     gene            complement(1500..1769)
                     /locus_tag="VIX62_12870"
     CDS             complement(1500..1769)
                     /locus_tag="VIX62_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861169.1"
                     /translation="MITREDLESLSSHQLHDRAIDMGKAEGDIDWLWSLLGSIPAAEG
                     QLGELDDSGLDIAHIVSAINGYVRSDRSTEDTLRPLYVDYLLEHL"
     gene            complement(1819..2721)
                     /locus_tag="VIX62_12875"
     CDS             complement(1819..2721)
                     /locus_tag="VIX62_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861170.1"
                     /translation="MAERSARATFVVVAAVVAAVMLAIPATATSRVAFSRSGDIFTVE
                     PDGSSARRITRRAATEHMPTWSPDHGRIAFLSWNRRIVVVDADGSGRRVLYRLPRRFE
                     GITGLAWSPDGSRLAFAIERRKNPDHPFGARDCGQIRWMSAAGGRTHVVINGEPHITG
                     ISWSPDGAWLAIGFEHQNMTRACGDDRPLGLARVRLDGSALHGIGPDLATDPDWRPDG
                     RVIAYRDWRRTCHACGEIWVVRPDGSRNHVLTPVPASEGGLRQPRYSPSGERLVALGD
                     GVVVLDRDGTIRRVIVEHATSVDW"
     gene            3221..>3463
                     /locus_tag="VIX62_12880"
     CDS             3221..>3463
                     /locus_tag="VIX62_12880"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY5861171.1"
                     /translation="MKHVRIATYEIRQGSFQEIADIAREGMLRTFQDQPGFIRYGLAD
                     LGGKSCLSFSMWETREDAEAAAPVATSWVRDHLSDRV"
BASE COUNT          551 a         1186 c         1199 g          527 t
ORIGIN      
        1 acaacacgga gcagatctcg atccgctcgg cgatccgcgc tgccgaggac gtcgcggccc
       61 agcacggagt ggcgacggac gacctcgcgt tcgatcgcct gatgctgcag tacgaagccg
      121 gcggaacgtc cgagggcgac ggaacgatgc gggaaccggt ggtgaccgag acgatcgtcc
      181 agttcaccca gatgatcgac ggcgtcccgg tgctggcgcc cgggcgggga cagctcagcg
      241 tgggtatcga caacgacggt cgcccgacga agatccggaa cacggtccgt ccgatcgccg
      301 aggtgaccga acgcgggcgc aggacgccgc cggtccccga ggccgaggcg gtcgcctcgg
      361 gtggtgatga catcgaaggc atgctgggcg atgcgtggca gaagcgcatg cgagcgttcc
      421 tcgtccagga tcggatgccg aggagcttcg cggaggtccc cggtaccacc gagatcggct
      481 acgtgatgga cggggacgaa gccgcgctcg tggtgcgtcg ggagtacgag gtcgatcacg
      541 gccgaggcat cctcaagcgg caccgcatcg aggtgccgct ggccgagtag acctcgcgct
      601 cgcgacggcg ccgtcggtct gcgagtcagc cgaacgggcg aaggctggat acctgactat
      661 gcgtcgcagc cgggcttcgt tagcctccgc ctagatcggg gaggcgcgtt ccctgaccct
      721 ggccccctcg gatctgcggg aggatccatg gggctcgacg ttcgccgcga ggcaccggtc
      781 ggcggtcggc cgcaccatca ggaccctcat cctccgccgt cgttccggct ctgtctcttg
      841 gggggcttcc agctcgccgt cgattacggc gtcgtggacg ttccgaccag cgtccagagg
      901 gtcgtgtcgt tcctcgctct gcacgatcgc ccgctgcagc gagccttcgt cgccggcacg
      961 tgttggcccg ccgcttcgga gaagcgggcg ctggcgagtc tccgctcggt cctctggcgg
     1021 ctccatcggc tcgggctcac cctggtcaac gcgtccaccg atcacgtgtg gctggacgtg
     1081 caggtcgagg tcgactatcg ggaggcgacc gccgtggcgc gggtggtcct ggagtcctcc
     1141 ggcgatctcg accccgagga actcaagctc ctgcagctcg gggagacgct gctcccggat
     1201 cggttcgagg attgggcggt gatggagcgt gagcgtctca tgcagctcag gctccacgcg
     1261 ctcgagcgcc tcagtgaacg cctcgtggcg gcgggcatgt acgccgaagc tgtggaggcc
     1321 gcgctcgccg ctgtggcggc cgaaccgctc agggagagcg cccaccgtgc gctgatcggc
     1381 gtgtatctcg ccgaaggcaa caccatcagc gcccatcgcc aatacgaggt gtgtcgccgg
     1441 ctcctcgacg acgaacttca ggtcgctcca tccaggctga tgcaggagct catgagcgac
     1501 tagaggtgct cgagcaggta gtcgacgtac aggggacgca acgtgtcctc ggtcgatcgg
     1561 tcggaccgca cgtagccgtt gatcgcgctg acgatgtggg cgatgtcgag cccggagtcg
     1621 tcgagctccc cgagctgtcc ttccgcggcg gggatcgagc ccagcaggct ccacagccag
     1681 tcgatgtcgc cctccgcctt ccccatgtcg atggctcggt cgtgcaactg atgggacgac
     1741 agcgattcga gatcctctcg ggtgatcatg cgtgcacggt actcggctcg tgaggcgtca
     1801 ccctgcgctg cgccggcctc accagtcgac ggaggtcgcg tgctccacga tcacgcgacg
     1861 gatcgtgccg tcgcggtcga gcacgacgac accgtcgccg agcgcgacga gccgctcgcc
     1921 gctcggcgag tatcggggct ggcggaggcc accctcggac gcgggcacgg gcgtgagcac
     1981 atggttgcgc gagccgtcgg gccgcacgac ccagatctcg ccgcacgcgt ggcaggtgcg
     2041 ccgccagtcg cggtacgcga tcacgcgacc gtcgggccgc cagtcggggt cggtcgccag
     2101 gtccgggccg atcccgtgaa gggcggaacc gtcgaggcgg acgcgcgcga gccccagtgg
     2161 ccggtcgtcg ccgcatgccc gcgtcatgtt ctggtgctcg aacccgatcg cgagccacgc
     2221 gccgtcgggg ctccacgaga tgccggtgat atgcggctcg ccgttgatca cgacgtgcgt
     2281 tcgaccgccc gcggccgaca tccaccggat ctgcccgcag tccctcgcgc cgaaggggtg
     2341 atcggggttc ttcctccgct cgatcgcgaa cgcgagacgg gaaccgtcgg gtgaccaggc
     2401 gagtccggtg atgccctcga accggcgggg cagacggtag agcacgcgtc gcccggagcc
     2461 gtcggcatcg acaaccacga tccggcggtt ccacgacagg aaggcgatgc gcccgtggtc
     2521 cggcgaccac gtcggcatgt gctcggtggc cgcccggcgg gtaatgcgtc tcgccgacga
     2581 gccgtcaggc tccaccgtga agatgtcgcc gctccgcgag aacgccaccc tcgacgtcgc
     2641 ggtcgccggg atggcgagca tgaccgccgc aacgaccgcg gcgaccacga cgaacgtcgc
     2701 tcgtgccgat cgttccgcca tgccgcctcc gaacccatgc cacgcccgtc ctgccgggcg
     2761 tccaggttca gggtcgcacg cagacgcaca acgcgctagg gtgaggcgac gcagctccga
     2821 agcggtcgga ccaagccgga ggagccaaca tcccccggtg gtccgcgacc taggacaccc
     2881 gtcgaacgca taggcccccg ggccttcggt tcctgtagcg gtgcatcgcg acgggacccc
     2941 gaccacccgg gggtcgctgc gcgactacct ctcccggccc cgcgatgacc tcacgacgcc
     3001 gcccgtcatc gccatcggac gaagacgtac actcgaggaa gacgcgaccc ccgtacctgt
     3061 cgggagaaga ccgtactgac ggggactcgc ctccatccgg tcccattcgc tggtcccagc
     3121 gaaggcctga taccccgagc gggtttcgct ctcgatctgg cttcacgttg gccccagatg
     3181 ttcaggggca acacgcatcg agcaaaggga gagcacacac atgaagcacg tgcgcatagc
     3241 gacctacgag atcaggcagg gatccttcca ggagatcgcc gacatcgccc gtgagggcat
     3301 gcttcggacc ttccaggacc agcccgggtt catccggtac ggcctcgccg acctcggtgg
     3361 caagtcctgt ctgtcgttca gcatgtggga gacccgcgag gacgccgaag cggcggcccc
     3421 tgtggccacg agctgggtgc gtgaccatct cagcgaccgg gtc
//
LOCUS       DATDBZ010000558         3508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_171613, whole genome shotgun sequence.
ACCESSION   DATDBZ010000558 DATDBZ010000000
VERSION     DATDBZ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171613"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..2384)
                     /locus_tag="VIX62_12885"
     CDS             complement(219..2384)
                     /locus_tag="VIX62_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861172.1"
                     /translation="MTEYRSVLERAGSNAPPPDLQVERVLRRRDRRQRNQRISAGALG
                     VVVALALGTILVRSVTSEPIPADPPVVPRPAPAAPGTLTYLLDGDIFVADPDGSNAVK
                     IANGRPDNECDRGSVGEQYWAEGSMWSPDGRYIAYRYDDCSNEGTGGVVISDAEGNVL
                     AKFPSGGWYIAWSPDSTRVAVWDSLDPPSTIGVYGLDGARQTQLTMPPGWTPRGDHDP
                     VWMPDGTSVWMDPWALPVDGSTPRQLPFGGGDSYATFSPDGSLVAYSDKRSLMVARSD
                     GSEPREVFGGGASDSAWSPSGDRIAFNDGESLSLLDVATGEVTLLTEKERETDLRIMG
                     FSPEGDRVLFSTIESDSLWSIGVDGSDASLVVAGTWQGEWLSQTTDEPTTADARPAPA
                     ASGTLAYALDGDIYVADPDGSNAVRIANGLPEEDCFDGGEYWAEGPMWSPDGRYLAYR
                     HNTDCSSSAPSWDVVISDAEGNVITTFPAAGWGVAWSPDSTRVAVWDRLYDPLVTDPF
                     NDPPATIGVYGLDGSRQTQLTMPSAWTSGENADDDPAWMPDGTSLRVHNLEVPLDGST
                     PRQLLLPLPALRWSVLSYSPDGSRVAYAADDDLSLVVAAADGSDARQVAGRTVVEPVW
                     SPTGDRIAFIHYEKGDKYGSSNELRVLDVATGEVTPLVGMDGSWDIGDIQFSPQGDRI
                     LISRYGRGADEYGLWSIGVDGSDARLVVAGTAQGDWLSR"
     gene            complement(2377..2901)
                     /locus_tag="VIX62_12890"
     CDS             complement(2377..2901)
                     /locus_tag="VIX62_12890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5861173.1"
                     /translation="MRETAQLGPAGREVHQASPPVAFEDFFAQEVDGLYRALWLVTRN
                     RFEAEELTQEAFVRVLERWDRVSEMEDPRAYLYRTGMNAFRTAYGRALLAAKRTMKAL
                     PADDAIAEIDERDVAVRALAPLSQRQRAAVVLTDMLGFRSEEAARMLGIRASTLRMHA
                     SRAHAALKEAMPRD"
BASE COUNT          569 a         1213 c         1165 g          561 t
ORIGIN      
        1 acacgacgtt cctcccgaaa gtacctccga gggggggctg tttcgagatt gggggtcgct
       61 ggacgcaggc ccgaacgcaa ctggatcgag aatcccaggc gcgcgcgggg ccggcagtac
      121 ctaccggggg gtactacttc aagattgggg acgtgttagc gcattctggg ctcaggagca
      181 tccgccactg ccctgtcacg gtgttgcacg tcacgcgatc agcgcgacag ccagtccccc
      241 tgggccgtcc ccgcgacgac gaggcgggcg tcggagccgt cgacgccgat gctccacagc
      301 ccatactcgt ccgctcctcg cccgtacctc gagatgagga tgcggtcgcc ctgcggtgag
      361 aactggatgt ccccgatgtc ccaggatccg tccatgccga ccagcggcgt cacctcgccg
      421 gtcgccacat cgagcacgcg gagttcgttg gacgacccgt acttgtcgcc cttctcgtag
      481 tggatgaagg cgatccgatc accggttggc gaccagacgg gttcgacaac cgtacgcccc
      541 gccacctgcc gagcgtcgga accatccgca gcggcgacga cgagcgacaa gtcgtcgtcg
      601 gcggcgtacg cgacgcgcga tccgtcgggc gagtaggaaa gcacgctcca gcgaagcgcg
      661 ggcaacggga gcaggagctg ccggggcgtg ctgccgtcga gcggcacttc caggttgtga
      721 accctcaacg aggtaccgtc cggcatccac gccgggtcgt catcggcgtt ctcgccactg
      781 gtccatgcgg acggcatcgt gagctgcgtc tgccgagacc cgtcgagccc gtagacgccg
      841 atcgtcgcgg gcgggtcgtt gaaagggtcc gtcacgagcg ggtcgtacag gcggtcccac
      901 accgcgacgc gtgtggagtc cggcgaccac gcgacacccc atcccgccgc tgggaacgtc
      961 gtgatcacgt tgccctccgc gtcgctgatc acgacgtccc acgacggcgc cgagctggaa
     1021 cagtccgtgt tgtgacgata ggcgaggtac cgcccgtcgg gcgaccacat cggtccctcg
     1081 gcccagtact cgccgccgtc gaaacagtcc tcttcgggaa ggccgttcgc gatcctgacg
     1141 gcgttcgagc cgtccggatc ggcgacgtag atgtcgccgt cgagcgcgta cgcgagggtg
     1201 cccgacgccg ccggggccgg ccgagcatcc gccgtcgtcg gttcatccgt cgtctgcgac
     1261 agccactccc cctgccatgt cccggcgacc acgaggctgg cgtcggagcc gtccacgccg
     1321 atgctccaca acgagtccga ttcgatcgtc gagaagagga cgcggtcgcc ctcgggtgag
     1381 aaccccatga tccggaggtc cgtttcacgc tccttttccg tcagcagcgt cacctctccg
     1441 gtcgccacat cgaggaggct cagggactcg ccatcgttga acgcgatccg atcgccggag
     1501 ggagaccatg cgctgtccga ggccccgccc ccgaacacct cccgaggttc ggaaccatcg
     1561 gatcgcgcga ccatcaacga acgcttgtcg gagtacgcga cgagcgatcc gtcgggcgag
     1621 aaggtggcat acgagtcgcc gcccccgaac gggagctgtc gcggcgtgct gccgtcgacg
     1681 ggcaacgccc atgggtccat ccacaccgag gtgccgtccg gcatccacac agggtcgtga
     1741 tcgccgcggg gggtccatcc gggcggcatc gtgagctgcg tctgacgagc gccgtcgagc
     1801 ccgtagacgc cgatcgtcga gggcgggtcc aagctgtccc acaccgcgac gcgtgtggaa
     1861 tccggggacc acgcgatgta ccaaccgccg ctagggaact tcgcgagcac gttgccctcc
     1921 gcgtcgctga tcacgacgcc cccagtaccc tcgttggaac aatcgtcgta gcgatacgcg
     1981 atgtaccgcc cgtccggcga ccacatcgat ccctccgccc agtactgctc gccgacgctg
     2041 ccgcggtcgc actcgttgtc gggacggccg ttcgcgatct tgactgcgtt cgagccgtcc
     2101 gggtcggcca cgaagatgtc gccatccagg aggtaggtga gggtgcctgg ggccgccggc
     2161 gccggcctgg gcacgacggg cggatccgcc gggatcggct ccgaagtcac cgagcggacc
     2221 aagatggtcc cgagcgcgag cgcgacgacg acgccgaggg cgcccgcact gatgcgctgg
     2281 ttgcgctgcc tgcggtcgcg gcggcgcagg acgcgctcca cctgcaggtc gggcggggga
     2341 gcgttcgatc ccgctcgctc gaggacggac cggtattcag tcacggggca tcgcctcctt
     2401 gagtgccgcg tgcgcccgag atgcatgcat gcggagcgtc gaggcacgga tgccgagcat
     2461 gcgagcggct tcctcggacc tgaagccgag catgtcggtc aggaccactg ctgcgcgctg
     2521 acgctgggag agaggggcga gggctcggac ggcgacgtcg cgctcatcga tctcggcgat
     2581 cgcgtcgtcg gcgggaagag ccttcatcgt ccgtttcgcg gccaggaggg cgcgcccgta
     2641 ggcggtgcgg aacgcgttca tcccggttcg gtacaggtag gcgcggggat cctccatctc
     2701 cgagacgcgg tcccaccgct ccagcacgcg aacgaacgcc tcttgtgtga gctcctcggc
     2761 ctcgaaccgg ttccgggtca cgagccacaa ggcgcgatac agcccatcta cctcttgcgc
     2821 gaagaagtcc tcgaaggcca cgggcggcga cgcctggtgg acttccctcc cagcgggccc
     2881 gagctgcgcc gtctctctca tcctgtaaca acaaccttcg gggaccccgg atcgcctaca
     2941 cggtgtgcga tcttttgttc agccagtgtc taacgccttg gcctcgcgca gcatcccgac
     3001 cagcaccccc tggagcgtcc ggtcctcctc gcagccgatc agggctcagc cgcccactgc
     3061 tcgacgagcg aggtcgagac ggtcggcgtt cctaccgggg ggcatcccaa agaacgcggg
     3121 tcgcctatcg ccggtaggtc aacggatcga agagccctag gcgcgctcgg cgggagacac
     3181 agtcgagttc ctccccaggg gggtagtcgc ttggctgcgc ccttcgcgcc catctccttc
     3241 gcgttctcgg tggtgaacct ccctccggtg tccgcttcat gcggtaggaa ctgcggatcc
     3301 gtgtccatga tctgaccacc tccatccaga gggtaagccg gtggtctggc gctccctcat
     3361 gctcaatgaa cggagcagaa gggccggaac cagacctgct ttccagcgac cccccgggac
     3421 accgtgggac acggcgagcc cgccagtcga tcacgttgat gcgggagtat cccttgcgct
     3481 gtaacggagc cggcggtcgg attcgaac
//
LOCUS       DATDBZ010000559         2859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_171815, whole genome shotgun sequence.
ACCESSION   DATDBZ010000559 DATDBZ010000000
VERSION     DATDBZ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171815"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VIX62_12895"
     CDS             <1..709
                     /locus_tag="VIX62_12895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY5861174.1"
                     /translation="RLGTVLDMHGSRDEIAANIATIRAGIEQACHEVGRTPHEVLLVA
                     AAKTLPAEVVRWAVEFGVTDIGENYVKELRQIHDDVPGARWHYIGALQTNTAHHVAAL
                     ADVVQTVAGLRATERLARRATEHGRTIDVLIEVDLTRERSGVAPDETVAFADRVAAME
                     GLTLRGLMTIPPLEHDAGSARPWFRELRALLDRVRDNHPDVVDLSMGMSLDYRAAVEE
                     GATMVRIGTALFGPRTP"
     gene            725..1195
                     /gene="sepF"
                     /locus_tag="VIX62_12900"
     CDS             725..1195
                     /gene="sepF"
                     /locus_tag="VIX62_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEY5861175.1"
                     /translation="MAAVWKKTLNYLGLVEEDEDFVDEMPEADPAPVRRMRPQAVREV
                     PAEADGIVRTIASPRPAVAATGAIHKSEPKRFNEAREVADRFKEGTAVIMNLQSTDDA
                     IARRLVDFASGLVYGLDGKIELVANRVYLLTPADVEVSAEERERLAGGGFYNQF"
     gene            1259..1537
                     /locus_tag="VIX62_12905"
     CDS             1259..1537
                     /locus_tag="VIX62_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY5861176.1"
                     /translation="MIAASPVVRLLCVALTVFWILLLVRMVLSWVELAGVRPPGTGPL
                     RSAYELLFDVTEPVLRPLRKLVPPAGMFDLSSLIAFVIILVLRMAICS"
     gene            1565..2299
                     /locus_tag="VIX62_12910"
     CDS             1565..2299
                     /locus_tag="VIX62_12910"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY5861177.1"
                     /translation="MGKRDKTTTDGSFQATPPPTASAPTRVVLGPLEIQAKEFRVSRF
                     GGYKMRDVDEFLDSVTDSMSALVAENDRLRKGAPAPVVGAPDLDDVSRQADEIIERAR
                     TEAARIVAGAKAQASGTASTPQDRAAVNAFLAKEREFLQSLAGLVQGHAESVKGMSKA
                     VRKPAAPAQTAPSAASPAPVSSTSTEAASDATAVQEAAEAAPATSIPEAAVSVVEPEP
                     AAVARSDDDGSAEGDRSLRDLFWGED"
     gene            complement(2510..>2859)
                     /locus_tag="VIX62_12915"
     CDS             complement(2510..>2859)
                     /locus_tag="VIX62_12915"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEY5861178.1"
                     /translation="VGGGVARDGGVTVALDLAITDDLRIEGTARELIRAVQDARKAAA
                     FEVADRVQLGVLAGPVVGAAISAHRDVIVAETLAVDLIVGEVDGYRQEMTIADEPLVI
                     SLQRAEWANPSTA"
BASE COUNT          460 a          956 c          972 g          471 t
ORIGIN      
        1 gaggttgggt accgtgctcg acatgcatgg gtcacgagac gagatcgccg ccaacatcgc
       61 gacgatcagg gcagggatcg agcaggcctg ccacgaggtc gggcgcacac cccacgaggt
      121 gctcctggtt gcggccgcca agacccttcc ggccgaggtc gtgcgatggg cggtcgagtt
      181 cggggtcacg gatatcggcg agaactacgt gaaggaactg cgacagatcc acgatgacgt
      241 gccgggcgct cgctggcact acatcggtgc gttgcagacc aacaccgcgc accatgtcgc
      301 cgcgctcgcc gatgtcgttc agaccgtggc gggcctgcgc gcgaccgagc gactcgcccg
      361 gcgagccacg gaacacgggc gcactatcga cgtgttgatc gaggtggatc tcacccgcga
      421 gcggtccggg gttgcgcccg acgagacggt ggcgttcgcc gatcgtgtcg ctgcgatgga
      481 gggcctcacg ctccgcggtc tcatgacgat ccctcccctc gaacacgacg caggatcggc
      541 tcgcccgtgg ttccgggaac tccgcgccct cctcgaccgc gtgcgagaca accaccccga
      601 cgtagtggac ttgtccatgg ggatgtcgtt ggattaccga gccgcggtcg aggaaggcgc
      661 tacgatggtt cgtatcggaa ccgctttgtt cggtccgagg acgccgtgat gtagggagga
      721 agtgatggcc gccgtgtgga agaagaccct gaactacctc ggactcgtcg aagaagacga
      781 agacttcgtc gatgagatgc ccgaggccga tcccgcgccg gttcggcgga tgcgtccaca
      841 ggcggttcgt gaggtgcccg ccgaggcaga cggcatcgtg cgcaccatcg catcgcctcg
      901 tccggcggtg gcagccaccg gcgccatcca caagtccgag ccgaaacggt tcaacgaggc
      961 ccgagaggtc gccgatcggt tcaaggaagg caccgctgtc atcatgaacc tgcagtccac
     1021 cgatgacgcc atcgcccgac gcctcgtcga cttcgcgagc gggctggtct acgggctcga
     1081 cggcaagatc gagctggtcg cgaaccgggt ctacctgttg acccccgcag acgtcgaggt
     1141 ctccgccgaa gagcgtgaac gtctcgcagg tggcggcttc tacaaccagt tctagcgcgc
     1201 cctcgagtcc cacgacatcg gtgtccggtt cccgggcgga ctgttagcct cgcacccgat
     1261 gatcgcggca tcccccgttg tacggctgct ctgtgtcgcc ctgacggtct tctggatcct
     1321 gttgttggtc cggatggtcc tgagttgggt cgagcttgct ggtgtgcgtc cacctggcac
     1381 cggcccgctt cgatctgcct acgagctgct gttcgacgtc accgagcccg ttctgcgtcc
     1441 gctgcgcaag ctcgtgccac cggcgggtat gttcgacctg tcgagcttga tcgcgttcgt
     1501 catcatcttg gtgctgcgga tggcgatctg cagctagggc agtcggggga accaggaggc
     1561 ttcgatgggc aagagagaca agaccacgac agacggatcg ttccaggcaa cgccgccgcc
     1621 gaccgcatcg gcgccgaccc gagtcgtgct cgggccgctc gagatccagg ccaaggagtt
     1681 ccgcgtctcg cggttcggcg gctacaagat gcgcgatgtc gacgagttcc tggactccgt
     1741 cacggattcc atgtcggcgt tggtggcaga gaacgatcgt ctccggaagg gggcgccggc
     1801 cccggtggtg ggtgcgcccg atctcgatga cgtgtcacgg caggccgacg agatcatcga
     1861 gcgcgcacgc accgaggcag cgcgcatcgt cgccggcgcc aaggcgcagg catctgggac
     1921 cgcctccacg ccgcaggacc gcgcggccgt gaacgccttc ctggcgaagg agcgggagtt
     1981 cctccaaagc ctggcgggtc tggttcaggg acacgcggag agcgtcaagg ggatgtcgaa
     2041 ggcagtcagg aaacccgcag cacctgcgca gacggccccg tcagcggcat ctcccgctcc
     2101 cgtctcatcg acgagtaccg aggcggcgtc cgacgccacc gccgtccagg aagcggccga
     2161 ggcggcgcct gcgacatcca tccctgaagc agcggtcagt gtggttgaac ccgagccggc
     2221 ggccgtcgcc cgctcagacg acgacggctc ggccgaaggg gaccgctcgc tccgcgatct
     2281 gttctggggc gaagactgac cggtccacaa ggaccagcct cgcacggccc gcatcgcgac
     2341 cgggtctacg gtcgcgacgt cgcgctgcgc ttcatggcag cgtggatcgc tgccctggtg
     2401 gctctggtga cgctggccgc tgggttgatc gtcgagcggg gcgccgtctg ggtgctcgcg
     2461 gccacggtcg ccgggttcgc ctcggtggtg ttggtgggtt cgggggtcct caggcggtgg
     2521 aggggttcgc ccactccgcc ctctgcaggc tgatcacgag gggctcatcg gcgatcgtca
     2581 tctcctgacg gtagccatcc acctcaccga cgatcagatc cacggcgagg gtctcagcga
     2641 cgatcacgtc tcgatgcgcc gagatggcgg cgcccaccac aggacctgcc agcacgccca
     2701 gctggacgcg atccgcgacc tcgaacgcgg cagccttccg ggcgtcctgg accgcccgga
     2761 tcagctcgcg tgccgttcct tcgatccgga ggtcgtccgt gatcgcgagg tcgagcgcga
     2821 ccgtgacccc accgtcgcgc gcgacccccc cccccaccc
//
LOCUS       DATDBZ010000560         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_808651, whole genome shotgun sequence.
ACCESSION   DATDBZ010000560 DATDBZ010000000
VERSION     DATDBZ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808651"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VIX62_12920"
     CDS             complement(<1..941)
                     /locus_tag="VIX62_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5861179.1"
                     /translation="MTDERAWLRSYPSDVPHSLAPYPKESLYMLLRNAAAGFPDRPAL
                     AFFGAHMTYAKLLSEVERFSAVLAGLGVHQGDRVGFILPNCPEYVIAFFACQRIGAVA
                     VGNNPLYTERELEHQIKDSGATVMIVLDQVYHRFGEVRAASGVKDVIAVKLNHYMAPP
                     IKWLAPLKFKSDAKKNGTPLPFIPVGHDVRWWADAMKHVGPIPPEATIVDPDTEVAAL
                     VYTGGTTGLSKGAMLSHANLVANARQASAWLNVVRQGEDGIVAALPFFHSFGTLVMDF
                     SMTKAAKLVLLPRFEIDMALRPWRRRSPRCSRASRACT"
     gene            1040..1630
                     /locus_tag="VIX62_12925"
     CDS             1040..1630
                     /locus_tag="VIX62_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861180.1"
                     /translation="MKRKTLLALTAVPLALLLSSCFSLQGFWIVANTIEAGGQATKAT
                     FQITPVRGSGDTAHQFFLIGVADSNDLQAGKGTWGTNQVFGGPYQLGVVANLAAIIGD
                     QCTSNGFNLGDVTGMTFKGYVTPNVVNDKNKVNKDVLVQVGLKAVDGATAGTEPVIGV
                     TGMWLDDDAGGVVGAPDASDGFICSGVSQVFVDVTA"
     gene            1815..>3286
                     /locus_tag="VIX62_12930"
     CDS             1815..>3286
                     /locus_tag="VIX62_12930"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5861181.1"
                     /translation="MSTTTPSEPTVQPVDRAIFRRGMKLVASYVRMHPKPFAASVAGS
                     VVFAFASLWLTFALAWATDEVLQPAFEGTLDTSKVWWAVGGLMLGGVGRALGIMVRRY
                     YSGVAGERVMATLRTRVAERYRSLSLRFHRETPTGELLAHMEADVKAAVDVFWPVPFA
                     VGVIVLTVLALIALVVTDPWMALVGCVVFPSLAILNRIFARRMEEPAQLAQERIGEVS
                     AVAHESIDGALVVKTLGREDAERDRLLERAQALRDARVQGGTIRATFEAALDALPTMA
                     IILLLAVGSARVASGDITLGDLIGFIALFQLLSWPMRFIGWILAELPRAVVGYDRIEE
                     VLREPVTVTPPREGIPLPDGPLDLHVHELRLEYEASSPVLEGVTFEVAAGESVAIVGQ
                     TGVGKSTLTQLMVRLVDPDTGHVEIGGVDLREVDVDSLRASVAVVFQESFLFAESVRS
                     NIALDSGAGEDAVVRAATIAACDRFIRALPRGYDTIVGERG"
BASE COUNT          513 a         1065 c         1131 g          577 t
ORIGIN      
        1 gtacatgcgc gggacgcccg ggaacaacgt gggcttctcc ttcgccatgg cctgagcgcc
       61 atgtcgatct cgaaccgcgg cagcagcacc agcttggccg ccttcgtcat ggagaagtcc
      121 atgaccagtg tgccgaacga gtggaagaac ggcagcgccg cgacgatccc gtcttccccc
      181 tggcgcacca cgttgagcca cgccgaggcc tggcgtgcgt tggccaccag gttcgcgtgc
      241 gagagcatcg cgcccttgga cagtccggtc gtgccaccgg tgtaaacgag ggcagcgacc
      301 tcggtatcgg ggtcgacgat ggtggcttcg ggcgggatcg ggcccacgtg cttcatcgcg
      361 tcggcccacc agcggacatc gtgcccaacg gggatgaacg gcaacggcgt gccgttcttc
      421 ttcgcgtcgg acttgaactt cagcggcgcc agccacttga tgggcggcgc catgtagtgg
      481 ttgagcttga cggcgatgac gtccttgacg cccgatgcgg cgcgaacctc gccgaaccgg
      541 tggtagacct ggtcgagcac gatcatgacc gtggccccgg aatccttgat ctggtgctcc
      601 agttcgcgct cggtgtacag cgggttgttg cccaccgcga ccgcgccgat ccgctggcag
      661 gcgaagaacg cgatgacgta ctcggggcag ttgggcaaga tgaagccaac gcggtcgccc
      721 tggtggacgc cgagacccgc gagcacggcc gagaaccgtt ccacttcgga caggagcttc
      781 gcgtaagtca tgtgcgctcc gaagaaggcg agcgccgggc ggtcggggaa ccccgccgcc
      841 gcgttccgca gcagcatgta cagcgattcc ttggggtagg gcgctagcga gtgcggcacg
      901 tcggacgggt aggagcgcag ccaagctcgt tcgtcggtca tgggcgcatc atcgccgaca
      961 cgcagggagt ttgcatagga cctccgacgg tgcgaccatc agagcttcca gcagctcgtc
     1021 ctagggggag gctttcgcca tgaagaggaa gacactgctt gcactgaccg cggtcccgct
     1081 ggcactgctg ctgagctcgt gcttctcgtt gcagggcttc tggatcgtcg cgaacacgat
     1141 cgaggccggt ggccaggcca ccaaagcgac cttccagatc acccccgtcc gcggttcggg
     1201 cgatacggcg caccagttct tcctgatcgg agtcgccgac agcaacgatc tacaggccgg
     1261 gaagggcaca tggggtacga accaagtgtt cggagggccg taccagctgg gcgtcgtggc
     1321 caacctcgcg gccatcatcg gagaccaatg cacctcgaac gggttcaacc tcggcgatgt
     1381 caccggcatg acgttcaagg gatacgtcac cccgaacgtc gtcaacgaca agaacaaggt
     1441 gaacaaggac gtcctcgtgc aggtcggcct gaaagccgtc gatggcgcaa cggcaggcac
     1501 cgagccggtg atcggggtga ccggcatgtg gctcgacgat gacgcgggcg gcgtcgtcgg
     1561 ggcacccgat gccagcgacg gtttcatctg ttccggcgtc agtcaggtct tcgtcgacgt
     1621 gactgcctga gtctcttcgc cccggaaggg gcagctgggt gcggttccgg ccgcgccacg
     1681 tgaacggttc ctgacgggtc gaccgcgcgc gcctccgctc gtgcggtctt ccgtcgtcct
     1741 gactccgtac gatggtcggt cgggctcgcc ttctgcccgc tcgcgacccg ggtcctcccg
     1801 agaggtttcc atccatgtcc acgacaacgc catctgagcc gaccgtgcaa ccggtcgatc
     1861 gtgcgatctt ccggcgcggc atgaagttgg tcgcctcgta cgtgcgcatg cacccgaagc
     1921 ccttcgctgc ctcggtcgcg ggctcggtcg tgttcgcgtt cgcatccctc tggctcacct
     1981 tcgcgctcgc gtgggcgacc gacgaggtcc ttcagccggc gttcgagggc accttggata
     2041 cctccaaggt ctggtgggcg gtcggaggcc tgatgctggg tggcgtgggc cgggccctgg
     2101 gcatcatggt gcgccgctac tactcgggcg tggccggcga gcgggtcatg gccacgctgc
     2161 gcacgcgtgt cgccgagcga taccggtcgc tctcgctccg cttccaccgt gaaaccccca
     2221 ccggcgagtt gctggcccac atggaagccg acgtgaaagc ggccgtcgac gtgttctggc
     2281 cagtgccgtt cgccgtgggc gtcatcgtgc tgaccgtgct cgccttgatc gccctggtgg
     2341 tgaccgatcc gtggatggcg ctggtggggt gcgtggtctt cccgtcactc gcgatcttga
     2401 accgcatctt cgcccgacgc atggaggaac ccgcccagct ggcgcaggag cgcatcggcg
     2461 aggtcagtgc ggtggcgcac gaatccatcg acggcgcgtt ggtggtcaag acgttagggc
     2521 gcgaggacgc cgagcgggat cgactgctcg aacgtgccca agccctgcgc gatgcccggg
     2581 tgcagggcgg gaccatccgc gccaccttcg aagcggcgct cgacgccctg cccaccatgg
     2641 ccatcatcct gttgctggcg gtggggtcgg cgcgggtcgc cagcggcgac atcacgcttg
     2701 gcgacctgat cgggttcatc gcgctcttcc agttgttgag ctggcccatg cgcttcatcg
     2761 ggtggatcct ggccgagctc cctcgagcgg tggtggggta cgaccggatc gaggaggtct
     2821 tgcgcgagcc cgtcacggtg acgccgccgc gcgaaggcat cccgctgccc gacggcccgc
     2881 tggacctgca cgtgcatgaa ctccgcttgg agtacgaggc ttcatcaccg gtgctcgaag
     2941 gcgtcacgtt cgaggtcgcg gccggcgagt cggtcgcgat cgtgggccag acgggtgtgg
     3001 gcaagagcac cctgacccaa ctgatggttc ggttggtcga tcccgacacc ggacacgtcg
     3061 agatcggcgg tgtggacctg cgagaggtcg acgtcgactc actgcgtgcc tcggtcgccg
     3121 tggtcttcca ggagagcttc ctgttcgccg agagcgtgcg atccaacatc gcgttggact
     3181 ccggagccgg cgaggacgcg gtcgtgcgcg cggcgacgat cgctgcctgc gaccgattca
     3241 tccgcgcgct ccccaggggc tacgacacga tcgtgggcga acgcgg
//
LOCUS       DATDBZ010000561         4795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2423527, whole genome shotgun sequence.
ACCESSION   DATDBZ010000561 DATDBZ010000000
VERSION     DATDBZ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423527"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..383)
                     /locus_tag="VIX62_12935"
     CDS             complement(222..383)
                     /locus_tag="VIX62_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861182.1"
                     /translation="MARFRYPDPLEPPPYPVTPSSSSPIAPSGEDQAAEAQPEEGITP
                     EAGDPATPD"
     gene            complement(446..607)
                     /locus_tag="VIX62_12940"
     CDS             complement(446..607)
                     /locus_tag="VIX62_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861183.1"
                     /translation="MAYRVFVTDGQVYEFPDHAAMVIDGSDYVFVVGDEEISRLACDD
                     VRDVEDSPA"
     gene            908..1318
                     /locus_tag="VIX62_12945"
     CDS             908..1318
                     /locus_tag="VIX62_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861184.1"
                     /translation="MRLVDGLLSRTSVQEERDFLERFSRELEDERAVLRSIVEGLDHD
                     KGLVQRGIGIATGVAAMAKTALPGSPDELEALEALAVGVWGKRLLWGALQTVADADER
                     FDGLPLGALARQAEDQERELVGLRQRAVAASLLC"
     gene            1354..1998
                     /locus_tag="VIX62_12950"
     CDS             1354..1998
                     /locus_tag="VIX62_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020519077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5861185.1"
                     /translation="MTDRIPPKRFHEVGWRIVRDDASLHFRTDSFGAGVALVDAIGKM
                     VETEHDRPAVDLRPDGVTVRLGSNREGRFSERDLVLAQQISGAARELGAAADLTGLQH
                     IQVAIDALVIPDVLPFWRAVLGYEQVGDEDLLDPHLEGPPLWFQQMDAPRPQRNRIHV
                     DLYVPHGEAETRVAAAIAAGGRVVNDANAPGWWTLADPEGNEVDVASWPDRIAS"
     gene            complement(2000..2073)
                     /locus_tag="VIX62_12955"
     tRNA            complement(2000..2073)
                     /locus_tag="VIX62_12955"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2037..2039),aa:Pro,seq:tgg)
     gene            2155..2565
                     /locus_tag="VIX62_12960"
     CDS             2155..2565
                     /locus_tag="VIX62_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861186.1"
                     /translation="MDAARPLHSSLTYNVLVIALVGMIGAGAALLLMTTGSPRAGARF
                     ELGTGEAVDCPFGTGSPVCYRFNMTNTGGAEGYARCVVVPGGDSTAIFSNGEVAYESP
                     VAVQPDEIYRLYARVDPGPSKEIAEPSITCGPAV"
     gene            complement(2569..3429)
                     /locus_tag="VIX62_12965"
     CDS             complement(2569..3429)
                     /locus_tag="VIX62_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY5861187.1"
                     /translation="MRVPFRLLDVFAEAPFAGNQLCVVSETPDGLDGETMQTLAHEIG
                     FSETTFVTAIRDGGYDVRIFTPEDEIGFAGHPTLGTAFALVEAGLVTSPVVQTSTAGD
                     VPVEVDLETNQATMRQLPPVFGPEVTDRDAVASAAGLQASDLVDGLPVVSASTGLAHL
                     MVAVNDEATLRRAERDDRGCGEVCAATDAESLYLFAVRGPGDVMARMFDRGITIGEDP
                     ATGSGAGPLGAYLSEHALAGMPGKVTVAQGEMVRRPSFLHVEVESAADSWAISVAGGV
                     RIVGEGAFEV"
     gene            3481..3554
                     /locus_tag="VIX62_12970"
     tRNA            3481..3554
                     /locus_tag="VIX62_12970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3513..3515,aa:Gly,seq:tcc)
     gene            3554..4129
                     /locus_tag="VIX62_12975"
     CDS             3554..4129
                     /locus_tag="VIX62_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019328248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5861188.1"
                     /translation="MARLIYASNMSLDGWTEDELGALDWAPPDEDVFVFITALMRSTG
                     TYLYGRRMYESMAVWETDSDLAARSDLTADFANAWQTADKVVYSSTLVATSTANTRLE
                     RHFDPKAVHDLKTVAETDLLVGGPNLAAQALAAGLVDELQLFVWPVVLGGRKPALPTD
                     TRAQLELLDEHRFGNGVVHLRYRVGDVINEG"
     gene            4139..4588
                     /locus_tag="VIX62_12980"
     CDS             4139..4588
                     /locus_tag="VIX62_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861189.1"
                     /translation="MRLRLLAPVIILAGGCRMAISPFVPYVLDDGTNAFIQARHNVNF
                     HGHYGEFFPGIPGAVIAVLALVALFSARWVKWPGVFAVAAVVAVGESAAALLLAQGFN
                     NEPIKYDTVMEALAVLAVGSGVVALGAMAAWVGRATSASSSPAASRA"
     gene            complement(4557..>4795)
                     /locus_tag="VIX62_12985"
     CDS             complement(4557..>4795)
                     /locus_tag="VIX62_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TolB protein"
                     /protein_id="HEY5861190.1"
                     /translation="ISTIDADGSDGLVVFDAGDRDVSTAVYSPDGLWIAFARDSRTGN
                     STDIWVVESDGGGAARATFGRSYDFMPGWQPVTS"
BASE COUNT          771 a         1646 c         1610 g          768 t
ORIGIN      
        1 aagagcggcg tggccggggc cgctccccga accgacgccc ccgcggccac caccccgatc
       61 gccgcgaagc acaacgcggc ggcaacacac aacgacgcga ggccagccac cttggtgcgt
      121 ccccgagttg acgtccgttc ccgccccgat gtcatcgcgc agacactagg ggatgacgga
      181 cgtgcatgga accaaccgcc ggcgacccga gccttcgcgc ctcagtcggg cgtcgccggg
      241 tcgcccgctt cgggggtgat cccttcttcg ggctgcgctt cggccgcctg gtcctctcct
      301 gatggagcga tgggcgacga gctgctgggg gtgacgggat agggcggtgg ttcgagggga
      361 tctggatagc ggaaccgtgc cacgggcgac cctccggttg tctgggatcg atgaataccc
      421 atgcggggca cgttgtatgc ctcggtcagg ccggcgagtc ctcgacatca cggacgtcgt
      481 cgcaggccag gcgagagatc tcctcgtcgc ccaccacgaa gacgtagtcg ctgccatcga
      541 tgaccatcgc ggcgtgatcc gggaactcgt acacctgtcc atccgtcacg aatacccgat
      601 acgccatgac ctccatcgtt tccggatcgg gaccggggaa acgccggcga tgtggtgaaa
      661 cggtgatgtc caggtcggcg ccatgatgga cggcttcgag gagacgtttc tccagcgtcc
      721 ccaaaggggt aaggcgacag cacgtgggcg tcaggatggc ggcctcgctg agcgaaggag
      781 cggaagcgat ggatctgacg cggatggact caccttggtg accgggacgg gttcggtcac
      841 ccgggacgtc gatgaccacg cactgtcgac gtacctggag cgacacctct cgggctccga
      901 tgcagccgtg aggttggtcg acgggctcct cagtcgaacc tccgtccaag aggaacgcga
      961 cttcctggag cggttctccc gggagctcga ggacgagcga gcggtgctgc gttcgatcgt
     1021 cgaaggcctc gatcacgaca aggggctcgt ccaacgaggc atcggcatcg cgacgggcgt
     1081 agcggcgatg gcgaagaccg ccctcccagg ttcgccggac gaactcgagg ccctcgaagc
     1141 gctggcggtc ggcgtttggg gcaagcggct cctctgggga gccctgcaga ccgtcgccga
     1201 tgcggacgag cgtttcgacg ggttgccgct cggtgcgctg gcgcgtcagg ccgaagacca
     1261 ggaacgggag ctggtcggcc tgcggcaacg ggccgtcgct gcctcgctat tgtgctgatc
     1321 gcgaacgtcg tgcgatgagc ggaggcgatc accatgaccg accggatccc accgaagcgg
     1381 ttccacgaag tcggctggcg gatcgtgcgc gacgacgcca gcctgcactt ccgcaccgat
     1441 tccttcgggg cgggggtcgc gttggtggac gccatcggca agatggtcga gaccgaacac
     1501 gatcgcccag ccgtcgacct ccggccagac ggcgtgacgg tgcggctggg gtcgaaccgc
     1561 gagggtcggt tcagcgagcg tgatctcgtc ctcgctcagc agatctcggg cgccgcgcgc
     1621 gaactgggtg ccgccgcgga cctgacgggc ctgcagcaca tccaggtggc gatcgacgcg
     1681 ctcgtcatcc ccgacgtctt gccgttctgg cgcgcggtcc tcggctacga acaggtcggc
     1741 gacgaggatc tgctggatcc ccatctggag gggccgccgc tctggttcca gcagatggac
     1801 gcaccgcgcc cccagcgcaa tcggatccac gtggatctct acgtgccgca cggcgaggcc
     1861 gagacgcggg tcgcggcggc gatcgccgcc ggcggtcgag tggtcaacga tgccaacgca
     1921 cccggctggt ggaccttggc cgaccccgag ggcaacgagg tcgacgtggc cagttggccg
     1981 gaccgcatcg cgtcctgagt cggggcggcg ggattcgaac ccgcgacctc ttgctcccaa
     2041 agcaagtgcg ctaccaggct gcgctacgcc ccgttccgct tcagggtacc ggtcgaagat
     2101 acggacttcc ggccgttgtg cggtcattca ggtcccgact agggtttcgc ccacatggat
     2161 gccgcccgtc ccctgcacag cagcctgacc tacaacgtac tggtcatagc gctggtcggc
     2221 atgatcggcg caggcgcggc gctgctgctc atgaccaccg gctccccgcg tgcaggcgcg
     2281 cggttcgagc tggggaccgg cgaagcggtc gattgtccgt tcggcaccgg ctcccccgtc
     2341 tgctaccggt tcaacatgac caacacgggc ggcgcggaag gctacgcccg gtgtgtggtc
     2401 gtcccagggg gcgattccac ggcgatcttc tcgaacggtg aggtcgcgta cgagagcccg
     2461 gtcgcggtcc agccggacga gatctaccgc ctctacgccc gggtcgaccc cggaccgtcg
     2521 aaggagatcg ccgagccctc gatcacgtgc ggtcccgcgg tctgacgctc agacctcgaa
     2581 cgcgccttcg cccacgatcc gcacgccgcc ggccaccgag atcgcccagg agtctgctgc
     2641 ggattcgacc tcgacgtgga ggaagctcgg tcggcgcacc atctcgccct gcgccaccgt
     2701 gaccttcccc ggcatgcccg cgagtgcgtg ttccgacaga taggcgccca gtggaccagc
     2761 gcccgaaccg gtggcagggt cctccccgat cgtgatcccc cggtcgaaca tgcgggccat
     2821 cacgtcacca ggcccgcgga ccgcgaacag gtagagggac tcagcgtccg tcgctgcgca
     2881 cacctctccg catccccggt cgtcccgttc ggcgcggcgc agggtcgctt cgtcgttcac
     2941 cgccaccatg agatgcgcca agcccgtcga cgcggagacc accggaagcc cgtcgacgag
     3001 atcggaggcc tgcaggccgg ctgcgctcgc aacggcgtcg cggtcggtga cctccgggcc
     3061 gaacaccggc ggcaactgcc gcatggtggc ctggttcgtc tcgaggtcga cctccaccgg
     3121 cacgtcgccg gccgtcgagg tctgcaccac cggcgaggtc accaggccgg cctccaccaa
     3181 cgcgaacgcg gtgcccagcg tgggatggcc ggcgaacccg atctcatcct ccggggtgaa
     3241 gatgcgaacg tcgtagccgc cgtcgcggat cgcggtcacg aacgttgtct cggagaatcc
     3301 gatctcgtgc gccagggtct gcatcgtctc cccgtcgagg ccgtcgggcg tctcggacac
     3361 cacgcacagc tggttgcccg cgaacggcgc ctcggcgaac acgtccaaca accggaaggg
     3421 aacccgcatg gcccggcagt atcgcaggcg atgagattgc taggcttggg aattagctac
     3481 gcgggtgtag ctcaatggca gagttccagc cttccaagct ggctatgcgg gttcgattcc
     3541 cgtcacccgc tccatggcga gactgatcta tgcgtcgaac atgtccctcg acggctggac
     3601 cgaggacgaa ctcggcgccc tcgactgggc tccacctgac gaggacgtgt tcgtgttcat
     3661 caccgcgctc atgcggtcga ccggcacgta cctctacggg cggcgcatgt acgagtcgat
     3721 ggccgtctgg gagaccgact cggacctggc cgcgcggtcg gacctcacgg ccgacttcgc
     3781 gaatgcctgg cagactgcgg acaaggtcgt gtactcctcg accctcgtcg cgacgtccac
     3841 cgcgaacacc cgcctcgaac gccacttcga cccgaaggcg gtccacgacc tcaagaccgt
     3901 cgccgaaacg gatctcctcg tgggtggccc gaacctcgca gcacaggctc tcgcggccgg
     3961 gttggtcgac gagctccaac tgttcgtctg gccagtcgtc ctcggcgggc gcaaacccgc
     4021 gctcccaacc gacacgcgtg cccagctcga gcttctcgac gaacaccgat tcggcaacgg
     4081 tgtcgtgcac ctccgctacc gcgtcggtga cgtgatcaac gaggggtagg gtcggcggat
     4141 gcgcctccgg ttgctcgcgc ccgtgatcat cctggccggc ggctgccgta tggcgatctc
     4201 gccgttcgtg ccgtacgtgc tcgacgatgg gacgaacgcg ttcatccagg ccaggcacaa
     4261 cgtcaacttc cacgggcact acggtgagtt cttcccgggc atccctggcg ccgtcatcgc
     4321 ggtcctcgcg ctggtcgcct tgttctcagc gcgatgggtc aagtggcctg gcgtcttcgc
     4381 ggttgcggcg gtggtggccg tcggagagtc ggcggccgcc ctcctgctcg cccagggttt
     4441 caacaacgag ccgatcaagt acgacacggt gatggaagcg ctcgcggtcc tcgccgtagg
     4501 ctctggcgtc gtcgccctcg gagcgatggc cgcgtgggtc ggacgggcga cctccgctag
     4561 ctcgtcaccg gctgccagcc gggcatgaag tcgtaggagc ggccgaaggt cgcgcgcgcg
     4621 gcgcccccgc catcagactc cacgacccag atgtccgttg agttgccggt gcgggagtcg
     4681 cgggcgaacg cgatccagag accgtccggt gagtacaccg ccgtgctcac atccctatcg
     4741 ccggcatcga acacgaccag cccatcggac ccatcagcgt cgatcgtcga gatgg
//
LOCUS       DATDBZ010000562         3627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1831072, whole genome shotgun sequence.
ACCESSION   DATDBZ010000562 DATDBZ010000000
VERSION     DATDBZ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831072"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VIX62_12990"
     CDS             complement(<1..881)
                     /locus_tag="VIX62_12990"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5861191.1"
                     /translation="MSATIEHTPATPATGYFRNPWRKPRILQGVTIGYLLWSLLPVVI
                     AVVFSFNAGRSRISWQGFSLRWWTQDPTDSLLRDEAMRAAMAQTYKLAILTVLIAVPL
                     GTLFAIGIDRWHGRPARAANFTMLLSFVVPEIILGVSLYILFTNLLQGLVPLGTPAQV
                     LGLISFQLSYPVIIVRARLLTIGPEYEEAAMDLGATPSQAIRRILLPLIAPAILASVA
                     LVFADVVDDFVTVAALSAEASSETLAQKIYSASRASPTPEINAAATVMLITTLLVVAG
                     GLLFYRRLSRGHGDIATT"
     gene            complement(878..1810)
                     /locus_tag="VIX62_12995"
     CDS             complement(878..1810)
                     /locus_tag="VIX62_12995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5861192.1"
                     /translation="MRRRRGSVEEPGRKSGAWHPRWYWPSFVAPGSLWLALMFVLPFY
                     VVFSVAFGTVDFESFGAPVPYYAPWWWSFDTFNDTLSKFYEGTAIYRPPLFRTFVYVF
                     SATVICLVVGYTVAYFAARAATKYKGLILIMLVSPLWISYLMRIYAWQGLLEVDGPIN
                     LLLQPLGLGDTNWLQGMHVTVILGLVYGYIPFMILPIYASLDRIQESLLEAGRDLGAS
                     GRQTFWRVTLPLSRPAILAGIVIVTLPMFGDYYTNDLLGSTKTSMFGNLISEQRLQNG
                     GSTRMASLVLILAVIVMAPMLYYLRETKRAAEQA"
     gene            complement(1854..3122)
                     /locus_tag="VIX62_13000"
     CDS             complement(1854..3122)
                     /locus_tag="VIX62_13000"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5861193.1"
                     /translation="MIGRHPMENRWSRRQFLGRAAGGAVALPSLSAILAACSKPGEDT
                     DPGSGSSASEIPIATLENPVELPVTMDTIAADTPIESGPLVLYNWADYIHKKVVAEFE
                     EEYGVEVEITTFNNMEEGLQKVANGQITPDVFVPTVGYLRRLVQKDLLLPLQHELIPN
                     MLASVWPSYSDPGPYYDLGWNYTVPYTIYTWGVAYRRDRVSDEEAAAQGWDALWNPEH
                     AGEISLYDSYGDAIAVAILRNGSLDVNTGDPALIDEAKVAILETINEHGARLTINGVY
                     AKIPAGDFTVAEAWSGDIVGAQWYLPKGTGTDVLGYWRPEPGQTMIGNDSLVVPAAAE
                     RPRLAHEFINFMLDEKHGTDNFVNWNGYQPPFVSIQPESLIDEGVVPENLSNAVVTEE
                     MFVDDLTPIELSPEVEALWLEAWTEIKAGA"
     gene            3263..>3627
                     /locus_tag="VIX62_13005"
     CDS             3263..>3627
                     /locus_tag="VIX62_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014212057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5861194.1"
                     /translation="MHTAPRVLVVGAGFSGLSAARRLADAGVDVTVLEARERVGGRVW
                     SVTLTNGAVVEMGAEWIMAGDTRVREVAQRFELALVETGASYGRREPWGAGAASLEAQ
                     DRFLEQANDAIAALPPERVA"
BASE COUNT          610 a         1161 c         1253 g          603 t
ORIGIN      
        1 gtcgtggcga tgtcgccgtg gccccggctc agccgtcggt agaagagcag gcctccggcg
       61 accacgagca acgtcgtgat cagcatcacc gtggcggcgg cattgatctc gggcgtcggt
      121 gaggcacgtg atgcagagta gatcttctga gcgagcgtct cgctactggc ctcagccgac
      181 agcgcggcga ccgtgacgaa gtcgtcgacc acgtcggcga agacgagagc gacgctcgcg
      241 aggatcgccg gtgcgatcag cgggagcaag atcctgcgga tcgcctggct cggcgtggcg
      301 ccgaggtcca tcgcagcttc ctcgtactcg ggcccgatcg tgagcaggcg ggcccgaacg
      361 atgatcaccg ggtagctcag ctggaagctg atgagaccga gcacctgcgc gggagtcccc
      421 aacgggacga gaccttggag caggttcgtg aacaggatat agagggagac acccaggatg
      481 atctccggta ccacgaacga cagcagcatc gtgaagttgg ccgcccgggc ggggcggccg
      541 tgccaccggt cgatgccgat cgcgaacagc gtgccgagcg ggaccgcgat gagcacggtg
      601 aggatcgcga gcttgtaggt ctgggccatc gccgcgcgca tcgcctcgtc gcggaggagc
      661 gagtcggtgg ggtcctgggt ccaccatcgc aaggagaacc cctgccacga gatgcgcgag
      721 cgaccggcgt tgaaagagaa gaccacggcg atcaccaccg ggagcaacga ccagagcagg
      781 tagccgatcg tcacgccctg caggatcctg ggcttccgcc acgggtttct gaagtacccc
      841 gtggcggggg tcgccggtgt gtgttcgatc gtggcgctca tgcctgctcc gcggcgcgct
      901 tggtctcgcg caggtagtag agcatcggcg ccatgacgat cacggcgagg atcagcacga
      961 gcgacgccat ccgcgtcgat cccccgttct ggagccgttg ctcggagatc aggttgccga
     1021 acatcgaggt cttcgtcgat ccgagcaggt cgttcgtgta gtagtcaccg aacatcggca
     1081 gcgtgacgat cacgatgccg gccaggatcg ctggtcgcga cagggggagc gtgacccgcc
     1141 agaaggtctg ccgcccgctg gcgccgaggt cgcgccctgc ctcgagcaag ctttcctgga
     1201 tgcggtcgag cgaggcgtag atcggcagga tcatgaacgg gatgtacccg tagacgaggc
     1261 ccaggatcac ggtgacgtgc atgccctgca gccagttcgt gtccccgagt ccgagaggct
     1321 gcagcaggag gttgatcggt ccgtcgacct ccagcaggcc ctgccaggcg tagatccgca
     1381 tcaggtagct gatccacagc ggcgagacga gcatgatgag gatcaggccc ttgtacttcg
     1441 tcgccgcgcg agccgcgaag tacgccaccg tgtagccgac cacgaggcag atcaccgtgg
     1501 ccgagaacac gtagacgaac gttcggaaca acggaggtcg atagatcgcg gtgccttcgt
     1561 agaacttgct gagggtgtcg ttgaaggtat cgaacgacca ccaccacggc gcgtagtagg
     1621 gcaccggtgc cccgaagctc tcgaagtcga ccgtgccgaa cgccacggag aagacgacgt
     1681 agaacggcag cacgaacatg agcgccagcc agagactgcc cggagccacg aacgagggcc
     1741 agtaccagcg tggatgccac gccccgctct tcctgcccgg ctcctcgacc gagccacggc
     1801 gccgcctcac gcggcgccgt ggtcgctcat cgtgtgtcgc cggtgtctcg atctcaggca
     1861 cccgccttga tctcggtcca ggcctcgagc cacaacgcct cgacctcggg cgagagctcg
     1921 atcggggtca ggtcgtcgac gaacatctcc tcggtcacga cggcgttgga caggttctcc
     1981 ggcacgacgc cttcgtcgat cagggactcg ggctggatcg acacgaacgg gggctggtac
     2041 ccgttccagt tcacgaagtt gtccgtgccg tgcttctcgt cgagcatgaa gttgatgaac
     2101 tcgtgggcga gccgcggcct ctctgcggcg gcaggtacga ccagtgagtc gttcccgatc
     2161 atcgtctggc ccggctcggg ccgccagtag ccgagcacgt cggtgccggt gcccttcggc
     2221 aggtaccact gcgcgcccac gatgtcgccc gaccacgcct cggcgacggt gaagtcgccg
     2281 gcagggatct tcgcgtagac cccgttgatc gtcagccgcg ccccgtgctc gttgatcgtc
     2341 tcgaggatcg cgaccttggc ctcgtcgatc aacgccgggt cgccggtgtt cacgtcgagg
     2401 ctgccgttgc gcaggatcgc gaccgcgatc gcgtcgccgt aggagtcgta gaggctgatc
     2461 tcgcccgcgt gctccgggtt ccacagggcg tcccagccct gcgccgcggc ctcctcgtcg
     2521 ctcacgcggt cgcgtcggta cgccaccccc cacgtgtaga tcgtgtaggg cacggtgtag
     2581 ttccacccga ggtcgtagta ggggccgggg tcggagtacg agggccagac gctcgcgagc
     2641 atgttcggga tcagctcatg ctgcaacggg agcagcaggt ccttctgcac gaggcggcgc
     2701 agatagccga cggtcggcac gaagacgtct ggcgtgatct gtccgttcgc gaccttctgg
     2761 agcccttcct ccatgttgtt gaaggtggtg atctccacct cgaccccgta ctcctcctcg
     2821 aactcggcga ccaccttctt gtggatgtag tcggcccagt tgtagagcac gagcggaccc
     2881 gattcgatcg gcgtgtcggc ggcgatcgtg tccatcgtca ccggcagctc gaccgggttc
     2941 tcgagggtcg cgatcgggat ctccgaagca gaggatcctg aacccggatc cgtgtcttct
     3001 cccggcttcg agcacgcggc gaggatcgcg gagagcgaag ggagtgcgac ggcgccgccc
     3061 gccgcccttc ccaggaactg ccgtcgcgac cagcggttct ccatcgggtg ccttccgatc
     3121 atggttgcct ccttgtcgca gcgcccaacg aagattgagc ggccgttcag gtcgatggcg
     3181 ctgagtacca taggcgcacc gtgccctgca agtgtcgccc atgcgcgccg cgctccggta
     3241 gtccacgggg accccgtcac tgatgcatac ggcgccgagg gtcctcgtgg ttggtgccgg
     3301 gttctccggc ctgtcggccg cccgtcgtct cgccgacgcg ggggtcgacg tcacggtgct
     3361 cgaggcgagg gaacgcgtcg ggggcagagt gtggtcggtc accctcacga acggcgcggt
     3421 cgtggagatg ggggcggaat ggatcatggc gggcgacacg cgggtgcggg aggtcgcgca
     3481 gcggttcgaa ctcgcgctcg tcgagaccgg tgcctcgtac gggcggcgtg agccatgggg
     3541 tgcaggcgcc gcgtcgctcg aggcacaaga ccgtttcctc gagcaggcga acgacgccat
     3601 cgcggctctt ccgcccgagc gagtggc
//
LOCUS       DATDBZ010000563         2053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2646612, whole genome shotgun sequence.
ACCESSION   DATDBZ010000563 DATDBZ010000000
VERSION     DATDBZ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2646612"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VIX62_13010"
     CDS             complement(<1..934)
                     /locus_tag="VIX62_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5861195.1"
                     /translation="MPDSDSSTPTPEPSSLHLETRAIRGGRDDNDTALAPILWATTTF
                     VTPTVEEGRRLATATGESRFYSRYGNPTVCAFESAIADLEGAEAARAFASGMGAVSAV
                     VLGLCSKGDHIVAQRQIYAGTQMLLQAACPRFGIDVTFVDGTEPGAFAAAVRPGKTVL
                     VLAETPANPQLDLVDLAELGAIAGPMTVVDSTFATPLAQRPLEHGVDLVVHSATKTLA
                     GHNDATLGVVAGSEELLQWLWGFAVLQGANASPFDAMNGLRGLRTLGVRFDRQCSTAL
                     AVASMLESHPAVSRVRYPGLASHPQHELACRQMDH"
     gene            complement(934..2025)
                     /locus_tag="VIX62_13015"
     CDS             complement(934..2025)
                     /locus_tag="VIX62_13015"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY5861196.1"
                     /translation="MHLPAERPFALEGGGVLRGVDVAYETWGELDADRSNAVLVCHAL
                     TGDSHVAGPSGRGHPTPGWWDDLVGPGRAVDTDRWFVICVNVLGGCQGTTGPASIDPD
                     SGRPFGALFPVVTIRDIVRTQAALADALGIDVWAAVIGGSMGGMQALEWAVMYPSRLR
                     AVAALATTGAAGAQQIAWSHVGRMAIAGDPRWRDGDYYDADAGDGPHQGLALARSIAQ
                     IHYRSEPVFEERFARRTVDPVDRLSLWDRFQVEAYLDYQGEKLVRRFDANSYLVLNKA
                     MDLHDLGRGRGGIDAALDRVSVPVLTLTIDSDSLYPPHQQAELRDALALRGRLLDHVE
                     VVSAHGHDGFLLEPAQVGAALSRLLEQVS"
BASE COUNT          350 a          696 c          723 g          284 t
ORIGIN      
        1 tgtggtccat ctgtcgacag gccagctcgt gctgtggatg cgacgccaag cccgggtacc
       61 gcaccctgga gacagccgga tgggactcca acatcgacgc gacagccaag gcagtcgagc
      121 actgacgatc gaaccgcact cccaacgtgc gaagcccacg aaggccgttc atcgcatcga
      181 agggggacgc gttcgcccct tgcaggacgg cgaaccccca cagccactgc aggagctcct
      241 cgctgcccgc caccacgccg agtgtcgcgt cgttgtgccc ggccagcgtc ttggtggccg
      301 agtggacgac caggtcgacg ccatgctcga gcggacgctg cgccaacggt gtcgcgaacg
      361 tggagtcgac cacggtcatg gggccggcga tggcacccag ctccgccagg tccaccagat
      421 cgagctgcgg gttcgcgggg gtctcggcca ggaccaggac ggtcttgcca gggcgaaccg
      481 ccgcggcgaa cgcgcccggc tcggtgccat cgacgaacgt gacgtcgatg ccgaagcgcg
      541 ggcacgcggc ctgcagcaac atctgggtgc cggcgtagat ctgacgttgg gcgacgatgt
      601 ggtcgccctt ggagcacagt cccagcacga cggcggagac cgcacccatc cccgacgcga
      661 acgcgcgggc cgcttcggca ccttcgaggt cggcgatggc gctctcgaac gcgcacacgg
      721 tggggttgcc gtacctgctg tagaagcggc tctcgccggt ggcggtggcc aatcgacggc
      781 cctcctccac cgtgggggtc acgaacgtcg tggtggccca caagatggga gccagggcgg
      841 tgtcgttgtc gtcacggccg ccgcggatcg cacgggtctc gaggtgcagg gatgagggct
      901 cgggagtggg tgtggacgag tcggaatcgg gcatcaggag acctgttcga ggagtcggga
      961 cagagccgcg ccgacctggg cgggttcgag gaggaagccg tcgtggccgt gggcactgac
     1021 gacctcgacg tggtcgagca ggcggccccg cagggcgagc gcatcacgca actctgcctg
     1081 ctggtgcggc ggatacagcg agtcggagtc gatcgtgagc gtgagcacgg ggacagagac
     1141 acgatcgagc gctgcgtcaa tgccgccgcg accacgaccg aggtcgtgca ggtccatggc
     1201 cttgttgagc accaggtagc tgttcgcgtc gaagcgacgg accagcttct cgccctggta
     1261 gtcgaggtac gcctccacct ggaagcgatc ccacagcgac aaccggtcga cgggatcgac
     1321 ggtgcgccgt gcgaagcgct cctcgaacac aggctcgctc cggtagtgga tctgcgcgat
     1381 cgagcgcgcg agcgcgagac cctgatgggg tccgtcgcct gcgtcggcgt cgtagtagtc
     1441 cccgtcacgc caccggggat cgccggcgat cgccatccgg ccgacgtggc tccacgcgat
     1501 ctgctgggct cccgctgcac ccgtcgtcgc gagcgcggcg actgctcgca gacgactcgg
     1561 gtacatcacc gcccactcga gcgcctgcat cccccccatc gagccgccga tgactgctgc
     1621 ccacacgtcg atgccgagcg cgtcggcgag cgctgcctgg gtgcgcacga tgtcgcggat
     1681 ggtcaccacc gggaacagcg caccgaaggg tcggcccgaa tccggatcga tcgacgcggg
     1741 gccggtcgtg ccctgacacc caccaaggac gttgacgcag atgacgaacc accggtcggt
     1801 gtccacggcg cgaccgggac cgacgaggtc gtcccaccaa ccgggcgtcg gatgccctcg
     1861 accgctcggg cccgcgacat gggagtcgcc ggtcagtgcg tggcagacca ggacggcatt
     1921 ggagcgatcg gcatcgagct cgccccaggt ctcataggcg acatcgacgc cgcgaaggac
     1981 acccccgccc tcgagcgcga acggacgctc ggcgggcagg tgcaggaacc ggcgatcacc
     2041 gacatcgtca ccc
//
LOCUS       DATDBZ010000564         2381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_173462, whole genome shotgun sequence.
ACCESSION   DATDBZ010000564 DATDBZ010000000
VERSION     DATDBZ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173462"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VIX62_13020"
     CDS             <1..418
                     /locus_tag="VIX62_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014744211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY5861197.1"
                     /translation="HFWDGTRDGELRIQRCQDCTKAYFPPRPFCPECSSRNVAVEVAS
                     GKAILYSYVINHREMPGFTAPYSIAVVELDEGPRMMTNIVECDQTPEALVLDMPVEVT
                     FEQVSDEISVPLFRPAEGRESRPAQGRESRPAEVAS"
     gene            415..1557
                     /locus_tag="VIX62_13025"
     CDS             415..1557
                     /locus_tag="VIX62_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HEY5861198.1"
                     /translation="MKQKSIAIVGAAETTEMGKIPDLSQLGLHADAALNALADAGLTA
                     KDIDGIATAGESPVNLAQYLGIVPTWVDGTSVGGCSFMLHVRHAAAAINEGLASTILI
                     THGESGRSRVGVGGFGMGGASQNGQFEMPYGPMGPPTMFTVPWLRYAKTYGATEVDLA
                     NVAVIQREWAALNPRASFKDPLTVDDVLGSRMIAWPFRKLLCCLVTDGGGALILTSAD
                     RAKDFPQKPVYILGTGESVETQMVSQMEDFTTSRAFRVSGKKAFDEAGITHADVDHLM
                     IYDAFAHLPIYGLEDLGFVGKGEAPAFIADRNTAIGGSLPLNTNGGGLSYMHSGMYGM
                     YALQESVRQMRGIAPAQVEGAEISIAHGVGGMFAASGTIVLTNTQP"
     gene            complement(1570..>2381)
                     /locus_tag="VIX62_13030"
     CDS             complement(1570..>2381)
                     /locus_tag="VIX62_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY5861199.1"
                     /translation="PTFSRQMTRRTPVYGLDIETDTTVDGLDPGVARILTVALSSDQV
                     DELFTGDEPSILRELDDRLASIEPGVIATWNGATFDLPFIADRAHLHGVPLGLRLEQD
                     SRIAVRGSALPGHTGAYRGSWHRHSHLDAYRVYRSDVGPALRISCGLKSIARFVGLPV
                     IEVDRTQIHDLSQEALHAYASSDARLARVLAERRWPSARRNIDREPLALASAVDHTRR
                     VTLMAEAARVAAERRAATTGHPIGVEVGPRIQIAQRRRQEAPVGDPLADAG"
BASE COUNT          396 a          801 c          820 g          364 t
ORIGIN      
        1 gcacttctgg gatggcactc gcgacggcga gctgcgcatc cagcgctgcc aggactgcac
       61 gaaggcgtac ttcccgcccc ggccgttctg tcccgagtgc tcgagccgca acgtggccgt
      121 cgaggtcgcc agcggcaagg cgatcctcta cagctacgtg atcaaccacc gtgagatgcc
      181 ggggttcaca gcgccctact ccatcgccgt ggtcgagctc gacgagggtc cgcgcatgat
      241 gaccaacatc gtcgagtgcg accagacccc cgaagcactg gtgctggaca tgccggtcga
      301 ggtgaccttc gagcaggtca gcgacgagat ctcggtgccg ctcttccgcc ccgcggaagg
      361 acgagagagc cgccccgcgc aaggacgaga gagccgtcct gcggaggtgg cctcgtgaag
      421 cagaagtcga ttgccatcgt cggagcggcc gagacgaccg agatgggcaa gatccccgat
      481 ctcagccagc tcggactcca cgccgatgcc gcgctcaacg ccttggccga cgccggtctc
      541 accgcaaagg acatcgacgg catcgccaca gccggcgagt ccccggtgaa cctcgcccag
      601 tacctcggca tcgtgccgac gtgggtcgac ggcacctcgg tcggcggctg ttcgttcatg
      661 ttgcacgtcc gccatgcggc cgcggccatc aacgaaggcc tcgcctccac gatcctcatc
      721 acccatggtg agagcggccg cagccgtgtg ggtgtcggtg gcttcggcat gggaggggcc
      781 agccagaacg gtcagttcga gatgccgtac gggccgatgg gcccacccac gatgttcacg
      841 gtgccgtggc tccgctacgc caagacctat ggagccaccg aggttgatct ggccaacgtc
      901 gcggtgatcc agcgggagtg ggcggcgctc aaccctcggg cgtcgttcaa ggatccgctc
      961 accgtcgacg acgtgctcgg ctcgcgcatg atcgcctggc cgttccgcaa gctgctctgc
     1021 tgcctggtca ccgacggcgg cggcgcgctc atcctcacct cggccgatcg ggcgaaggac
     1081 ttcccgcaga agcccgtcta catcctcggc accggcgaga gcgtcgagac ccagatggtg
     1141 agccagatgg aggacttcac cacatcgcgc gccttccgcg tgtcgggcaa gaaggccttc
     1201 gatgaggcag gcatcaccca cgccgacgtg gatcacctga tgatctacga cgccttcgcg
     1261 cacctgccca tctacgggct cgaagacctg ggattcgtcg gcaagggcga ggcgccggcg
     1321 ttcatcgccg atcgcaacac cgccatcggc ggttcacttc cgctcaacac caacggcggt
     1381 ggcctgtcct acatgcactc cgggatgtac ggcatgtacg ccctccagga gagcgtccgt
     1441 cagatgcgtg gcatcgcccc cgctcaggtg gagggtgcgg agatctcgat cgcccacggt
     1501 gtcggcggca tgttcgccgc cagcggcacg atcgtgctca cgaacaccca gccgtagggc
     1561 gggagcggat cagccggcgt cggccagggg gtctccgacg ggagcctcct gccggcgccg
     1621 ctgcgcgatc tggatgcgcg gtcccacctc gaccccgatc gggtgcccag tggtggcggc
     1681 tcgccgttcg gccgcgacgc gtgccgcctc ggccatcagc gtgacgcggc gcgtgtggtc
     1741 cacggcgctg gccaacgcca gtggttcgcg gtcgatgttc cgccgggcag acggccaccg
     1801 ccgctcggcg agcacccggg ccagacgggc gtcgctcgac gcgtaggcgt ggagcgcctc
     1861 ctgggagagg tcgtggatct gcgtgcgatc cacctcgatg accgggagtc cgacgaagcg
     1921 ggcgatcgat ttgagcccac aggagatgcg cagggccggc ccgacgtcgg accggtagac
     1981 ccgatacgcg tccaggtggg agtggcggtg ccacgaacca cggtaggcac cggtgtggcc
     2041 aggaagcgct gagccgcgca cggcgatgcg ggagtcctgc tcgagacgca atccgagtgg
     2101 cacaccgtgg agatgcgcgc ggtcggcgat gaacgggaga tcgaaggtcg cgccgttcca
     2161 ggtggcgatg acgccgggct cgatggatgc caggcggtcg tcgagctcgc ggaggatcga
     2221 cggctcgtca ccggtgaaca actcgtccac ctggtcgctg gacagcgcca cggtgaggat
     2281 gcgggcgacg ccagggtcga gaccgtcgac agtggtgtcc gtctcgatgt cgagcccgta
     2341 gaccggcgtc ctccgtgtca tctgcctgga aaaggtaggg a
//
LOCUS       DATDBZ010000565         2343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_173766, whole genome shotgun sequence.
ACCESSION   DATDBZ010000565 DATDBZ010000000
VERSION     DATDBZ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173766"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VIX62_13035"
     CDS             <1..524
                     /locus_tag="VIX62_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY5861200.1"
                     /translation="SRISTEFVNLTERHFESLGIPVKVRWSYGATEAKVPEIVDAIVD
                     VTETGSTLRAHGMKIIATLLASEPVLVANREAAADPVKRAAMDDITTLLLGALQAEGR
                     VLIKLNVSDAARDAELAVVPSMKAPTVSPLTDGGFAIETVVDKRGVNRLIPELKAAGA
                     TDILELPISKIVP"
     gene            580..1863
                     /locus_tag="VIX62_13040"
     CDS             580..1863
                     /locus_tag="VIX62_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861201.1"
                     /translation="MKPAIRTSVIAACVGIASLIAAPADAKPGQLDHFFSGDGRQTAF
                     ASGGSGYAVTLDDAERIVVAGYTLGHNTNIALVRLLPNGTFDPDFGGGDGRVVTNLGG
                     TDYGFDVAIHPDGGIVVAGERVSGSGSRVAVLRYGPKGRLDKDFGGGDGVVLTTFGKK
                     TQGASAVVIGASGNITIGGYTSNGTTSRFALARYGPRGVLDKDFGRKGLVTTDITPAG
                     EGIKDMVMVDGGKIVVTGFAEVGLIPQFAVARYLVRGGLDRTFGTKKGYSITDLSKGP
                     DTAYGISEGPDGQLLTCGLAGNRRRNDWGLARYGAKGRLDGSFSGNGKTIVNMGKGFG
                     SAYGCATLRSGKSVVVGRGRRTGKGEEFAVARLKVNGGLDGTFGKQGKAHADFFDGND
                     TARDVVIQSNGKIVVVGDATERGVIRMGMARFING"
     gene            2141..>2343
                     /locus_tag="VIX62_13045"
     CDS             2141..>2343
                     /locus_tag="VIX62_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947706.1"
                     /note="catalyzes the reversible formation of glyoxylate
                     and succinate from isocitrate; glyoxylate bypass pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEY5861202.1"
                     /translation="MTEAIEQRIREQAADLERRWSTDPRWKGVERTYTAEDVIRLQGS
                     FHVEHSIARAGAERLWELVHNTE"
BASE COUNT          416 a          783 c          807 g          337 t
ORIGIN      
        1 actcgcgcat atccactgag ttcgtcaacc tcaccgaacg gcacttcgag agcctgggta
       61 tcccggtgaa ggtgcgctgg agctacggcg ccaccgaggc gaaggtgccc gagatcgtcg
      121 acgccatcgt cgacgtcacc gagacgggca gcacgctgcg ggcccacggt atgaagatca
      181 tcgcgacgct gctcgcctcc gagccggtgc tggtggccaa ccgcgaggcc gccgccgacc
      241 cggtcaagcg cgcggcgatg gacgacatca ccaccctgct cctgggtgcc ttgcaggccg
      301 agggccgagt gctgatcaag ctcaacgtct cggacgccgc gcgcgacgcc gagctcgcgg
      361 tggtgccgtc gatgaaggcc cccaccgtgt ccccgctcac cgacggtggc ttcgcgatcg
      421 aaacggtggt ggacaagcgg ggggtcaacc ggctgatccc cgagttgaag gccgccggcg
      481 ccaccgacat cctcgaactg cccatctcca agatcgtgcc ttgaccgttc ccccgcagcg
      541 cacctaccct cccagggcat ccaccaggag ggaccagccg tgaagcccgc gatcaggacg
      601 tccgtcatcg ccgcgtgcgt gggcatcgcg tccctcatcg cggcgccggc cgacgccaag
      661 cccggccagc tcgaccactt cttcagcggt gacgggcgcc agaccgcgtt cgcgagcggt
      721 gggagcggct acgcggtgac cctcgacgat gcagaacgca tcgtcgtcgc cggctacacc
      781 ctcggacaca acacgaacat cgcgctcgtg cgtctgctgc cgaacggcac gttcgatccc
      841 gatttcgggg gcggggacgg ccgcgtcgtc accaacctgg gcggcaccga ctacggcttc
      901 gacgtggcga tccatccgga cggcggcatc gtcgtcgcag gagagcgggt gtcgggttcc
      961 gggtctcggg tagccgtcct gcgctacggc cccaaggggc ggctcgacaa ggacttcggc
     1021 ggcggtgacg gcgtcgtgct caccaccttc gggaagaaga cgcagggagc cagtgcggtc
     1081 gtcatcggag cgagcggcaa catcacgatc ggcggctaca cctcgaacgg gacgaccagc
     1141 cgcttcgccc tggcccggta cgggcctcgc ggcgtgctcg acaaggactt cggccgcaag
     1201 ggactcgtca ccaccgacat cacccccgcc ggggagggca tcaaggacat ggtgatggtg
     1261 gacggcggca agatcgtggt gaccgggttc gccgaggtgg ggctgatccc gcagttcgcg
     1321 gtggcgcgct acctggttcg cggcggcttg gaccggacct tcggcacgaa gaagggctac
     1381 agcatcaccg acctgtcgaa gggacccgac accgcctacg ggatctcgga aggccccgac
     1441 ggccagctgc tgacgtgcgg gctggccggc aaccgccggc gaaacgactg gggcctggcg
     1501 cgctacggcg cgaagggacg actcgacggc tcgttctcgg gcaacggcaa gacgatcgtg
     1561 aacatgggca agggcttcgg atcggcctac ggctgcgcca ccctgcgcag cggcaaatcc
     1621 gtcgtggtgg gtcgtggacg gcgaaccggc aagggcgagg agttcgccgt ggcccggctg
     1681 aaggtcaacg gcggcttgga cggcacgttc ggcaagcagg gcaaggccca cgccgacttc
     1741 ttcgacggca acgacaccgc ccgtgacgtg gtgatccagt ccaacggcaa gatcgtggtc
     1801 gtgggcgacg ccaccgagcg aggcgtgatc cggatgggca tggcccggtt catcaacggt
     1861 tgaccagcag tcagggcctg gttcgaacga gcctttcggc cgtttgacac ctcgggggtc
     1921 aggtgtggca caatcgggcc acgatgtttg aaccgacgtt caacaccacg ttcgaacgcg
     1981 tatccggtac gtaggggcgc acccggacgc gtgagcgtcc atgtgcgcca gggggacctc
     2041 tcgtcatcga gaggtccttt cgtttcccgg cagcgttccc gctccacccg atcaggcttc
     2101 acagaccaag gagcaccacg aaccaagggg gcaacaggcg atgacagagg cgatcgagca
     2161 gcggatccgc gaacaggcgg cggacctcga gcggcggtgg agcaccgatc cgcggtggaa
     2221 gggcgtcgag cgcacgtaca ccgccgagga cgtgatccgg ctccagggtt cgttccacgt
     2281 cgaacattcg atcgcccgtg cgggagccga gcgcctctgg gagctggtcc acaacaccga
     2341 ata
//
LOCUS       DATDBZ010000566         2611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2839185, whole genome shotgun sequence.
ACCESSION   DATDBZ010000566 DATDBZ010000000
VERSION     DATDBZ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2839185"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..689
                     /gene="nadD"
                     /locus_tag="VIX62_13050"
     CDS             81..689
                     /gene="nadD"
                     /locus_tag="VIX62_13050"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY5861203.1"
                     /translation="MGGVRTRLGVMGGTFDPIHYGHLVTAEEALHQFDLDEVLFVPTG
                     RPWMKAHEAVSPAEDRYLMTVIATASNPLFGVSRIEVDRDGPTYAVDTLRELRTERGD
                     DTDLFFVTGADAVLEIVEQWKEPEELLALAHFIAATRPGFDLEAFEAHAPTKHPRITV
                     MNIPALAISSTDVRARVHASRPIRYLVPAGVKSYIEKAGLYR"
     gene            686..1783
                     /locus_tag="VIX62_13055"
     CDS             686..1783
                     /locus_tag="VIX62_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEY5861204.1"
                     /translation="MTDQRAPGEPVRSSRVERASASRRLRGVVWGVVAALVVAAVGWY
                     LVGRGDDPPAAADDMGAGQPAPEASTLLALQVNGAPAPMLAVIGVPGDGEPFLMPLST
                     ELTVTMPGQGETSMAGVAALPGDSMRVALSNMSGVWIEHFAVLTLRDIAAAVDAAGGL
                     AVNLPASYPTTTDVLGPGEITMTGSQVKSFLAGATDDAGVRWEILLTAMLADPIPLVT
                     TPSVEADDGEAVNETVAAAGGAEVLTMPTERVTATIILPIYPSLDEMLSRRLDTPVPV
                     STIVQNGSGEPGVGEAVAVQIIPAGFRVVLSQNAQTFDLTHTDVFANGPDHEGEARAI
                     KAALGVGRVRVAAVPSNVGDITIVVGKDFTA"
     gene            1780..2193
                     /gene="rsfS"
                     /locus_tag="VIX62_13060"
     CDS             1780..2193
                     /gene="rsfS"
                     /locus_tag="VIX62_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY5861205.1"
                     /translation="MSQQQGTTTGGAQALPPSRDVAIAAARAAADKQATEIVVLDVSK
                     IIVITDHFVICSAGTQRQIRSVIDAVEASLRDLGVKPMRREGEAEAGWWLLDYVDVVV
                     HVFGEEERAYYDLERLWSDAPKVEWQPSDAASGAR"
     gene            2208..2280
                     /locus_tag="VIX62_13065"
     tRNA            2208..2280
                     /locus_tag="VIX62_13065"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2241..2243,aa:Ala,seq:ggc)
BASE COUNT          412 a          911 c          892 g          396 t
ORIGIN      
        1 cgggctttcc tgggggagcc cagcccgtga gcgagacgac cgtcacgacg ctcccggaga
       61 cgtcgggcct gcccactagg atgggcggcg tgaggacgcg cctgggagtc atgggcggca
      121 cgttcgaccc catccactac gggcatctcg tcaccgccga ggaagcgctg caccagttcg
      181 acctcgacga ggtgttgttc gtgccgaccg gtcgtccgtg gatgaaggcc cacgaggccg
      241 tctcgcccgc cgaagaccgc tacctgatga ccgtcatcgc cacggcctcg aaccccttgt
      301 tcggcgtctc acgcatcgag gtcgaccgcg acggcccgac gtatgcggtc gataccctgc
      361 gcgagttgcg caccgagcgt ggcgacgaca cggacctgtt cttcgtgacc ggcgccgacg
      421 cggtgctcga gatcgtcgag cagtggaagg aacccgagga actgctcgcc ctcgcccact
      481 tcatcgcggc gacccgcccc gggttcgacc tcgaagcgtt cgaggcgcac gcgccaacca
      541 agcacccgcg catcaccgtg atgaacatcc ccgcgctcgc catctcctcg accgacgtgc
      601 gagcccgggt gcacgcgagc cgaccgatcc gctacctggt gcccgcgggc gtgaagagct
      661 acatcgagaa ggcggggctc taccggtgac cgaccagcgg gccccgggcg agccggtgcg
      721 ttcgtcgcgg gtcgagcgcg cctcggccag tcgccgccta cgcggcgtcg tgtggggcgt
      781 cgtggccgcc ctcgtggtgg cagcggtcgg ctggtacctc gtcgggcgag gtgacgaccc
      841 acctgcggcg gccgacgaca tgggtgcagg tcagccggcg cctgaggcgt cgacgctgct
      901 cgccctgcag gtgaacggcg cccctgcgcc gatgctcgcg gtgatcggcg tgcccggcga
      961 cggcgagcct ttcctgatgc ccttgtcgac ggagctcacc gtcacgatgc ctggccaggg
     1021 cgagacatcg atggcgggcg tggccgccct gcccggcgac tcgatgcggg tggcgctgtc
     1081 gaacatgagc ggcgtgtgga tcgagcattt cgccgtgctc accctgcgcg acatcgcggc
     1141 agcggtggac gcggcgggcg ggttggccgt gaacctgccg gcttcctacc cgaccaccac
     1201 cgacgtgctc ggcccgggtg agatcacgat gaccggttcg caggtgaagt cgttcctcgc
     1261 aggtgcgacc gacgacgcgg gggtgcggtg ggagatcctg ctcacggcga tgctggccga
     1321 ccccatcccg ctcgtgacga ccccctcggt cgaggccgac gacggtgagg cggtgaacga
     1381 gaccgtcgcc gctgccggcg gcgccgaggt gctcacgatg cccaccgaac gcgtgacggc
     1441 cacgatcatc ctgccgatct acccctcgct cgacgagatg ctctcccggc gtctcgatac
     1501 ccccgtgccc gtctccacga tcgtgcagaa cggcagcggt gagccagggg tgggcgaggc
     1561 cgttgccgtg cagatcatcc ccgccggctt tcgagtggtg ctgtcgcaga acgcccagac
     1621 cttcgacctc acccacaccg acgtgttcgc caacggcccc gaccacgagg gtgaggcgcg
     1681 cgcgatcaag gcggccctgg gcgtcggccg ggttcgcgtg gcggcggtac catcgaacgt
     1741 cggcgacatc acgatcgtgg tggggaagga cttcacggct tgagccagca acagggcacg
     1801 acgacaggcg gcgcgcaggc gctgccgccc agtcgcgacg tggcgatcgc cgcagcccgt
     1861 gccgccgccg acaagcaggc caccgagatc gtggtgctcg acgtctcgaa gatcatcgtc
     1921 atcaccgatc acttcgtgat ctgctcggcc ggcacccagc gccagatccg ctcggtgatc
     1981 gacgccgtgg aagccagcct gcgcgatctc ggcgtgaagc ccatgcgccg cgagggcgag
     2041 gccgaggcgg gctggtggct gctcgactac gtcgacgtcg tcgtgcacgt cttcggcgag
     2101 gaggaacgtg cctactacga cctggagcgc ctgtggtccg atgccccgaa ggtcgagtgg
     2161 cagccctcgg acgccgcttc cggcgcccgt tagaatcacg cgaacgaggg cccatagctc
     2221 agttggtaga gcgcttggct ggcagccaag aggtccgggg ttcgaatccc cgtgggtcca
     2281 ccgggatagc ccacgttcaa gacggatctc aacagcatgg atcccgctca ggcgtggcgc
     2341 cgttcgatcc actcctgcgg ccaagcgacc agcaagggcg atcgctccca cacgacatga
     2401 acgaccccct caccgaggct ctcgacaacg ccgtgttctc catccccggg ccactgcaga
     2461 acggggccgc acggaccgat gaactcgacg aggtcatgcg ggcgcaatgc atcgtgttcc
     2521 atcggaacgg ttatcggcag tgttgtcggc cagttgagcg agctcggcat cgcccgatcg
     2581 gccgacaaag gatcccggct cggttcgatc c
//
LOCUS       DATDBZ010000567         3958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2426114, whole genome shotgun sequence.
ACCESSION   DATDBZ010000567 DATDBZ010000000
VERSION     DATDBZ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426114"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2010)
                     /locus_tag="VIX62_13070"
     CDS             complement(<1..2010)
                     /locus_tag="VIX62_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861206.1"
                     /translation="MSIYRAFRALLASSLVMGACMLPWPGPALAQEPGVTLTLISQTP
                     ITTQEEPLLQVTVQATNTGDTALDVLSVGISIGTEIDSRFVYERSLIDGPGPTLNFAT
                     ARPQEGSLEPGQQRLFDINLNMVDQVTGVIETPDSAVYPAQFDIRADGVVQASLNTPL
                     IHLVQAPEQPMLLSWWSELTGPIAFGPDGRLTDRSIETSVTPEGTLGAQVAALLRLVE
                     TPGHGAIDLAVEPALLDQLVRMGDGYERGDGSVIGRDEKPATDAAALVRDLRTVAADP
                     DVQISAMPFSAPLMPAMLAGGLSVDLEGQRVLGERTVVEVLGPAGQPTTSFERPPAGA
                     IDDATMDDLAIRGVGAVLANADAVDRTAQLNDFAPLPAASLTLASGSSIDLVLPDPAV
                     QALLADPVLVSDPVRGAQAVLGELATIWREQPVPADQPNQTETVRGVAVALSASLPPG
                     MWAPMMRRLADSPFLEPTHPQAFVETVNPLQIASTLAAPSEARFEDDYAEKIRSERRD
                     VEAYRSMLAEPSTTPDRLRRDLLYAEAGVYIDDPLAGRRWIDQVNAFTDHVFARALPA
                     DPQSFTLTSGEGQLPLRMGDPGETPLIVQIQLRSSQFEFPDGNERTVTLREPDQIVTF
                     TVEAKTSGTQTIRMRTRAPSGRPLDERNLAVRTTAVNSIALVITGA"
     gene            complement(2040..2819)
                     /locus_tag="VIX62_13075"
     CDS             complement(2040..2819)
                     /locus_tag="VIX62_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5861207.1"
                     /translation="MTPLDVVLGIAVGFVSGVLSGLFGVGGGIVMTPGIQVLMGARPI
                     VALATPLPAILPTALTGAWTYHRAGQVDVRAAAWMVGPGIAGAVAGALLTDVVNTRVL
                     LVVTALLLAWQAIGILRGARTRPAEDPEVARAPEPWMFLVIGLAAGFVSGLLGIGGGL
                     IMVPLMVGWLGMPLKVALGTSLLTIVALVVPGTVVHVMLGHIDWAICLVVTLGAVPGA
                     RLGAGFALDTKERTLRLMVGSFLLVVAVLYGATELAALLRG"
     gene            complement(2816..3235)
                     /locus_tag="VIX62_13080"
     CDS             complement(2816..3235)
                     /locus_tag="VIX62_13080"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5861208.1"
                     /translation="MQKEVSAGGVVYRRLGDEIEVVLASRRTRKGELAWGLAKGGIEE
                     GETHEQAAVREVKEETGYTAEIEASLGETKYFYVWEDTRIRKTVHFFLMRCTGGDPDD
                     RDDEMEEVRWFPLERALKRAAYRGERDVLGRAADLLR"
     gene            3513..>3958
                     /locus_tag="VIX62_13085"
     CDS             3513..>3958
                     /locus_tag="VIX62_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5861209.1"
                     /translation="MEIDELLRFAVERGSSDLHLKVGNVPFIRVDGALTPTEFDVLTS
                     ADTETFAQALMSDHKKREFEATSEADLGTTLTGVGRFRVNVFRQRGVVGLAIRRVRSE
                     VPTFDELRLPPVMETLADSPRGLVLITGPTGTGKTTTIASMIGHINR"
BASE COUNT          646 a         1307 c         1362 g          643 t
ORIGIN      
        1 cgctccggtg atcaccaggg cgatcgagtt gacggctgtc gtgcgcaccg ccaggttgcg
       61 ttcgtccaac gggcgtccgg acggcgcgcg ggttcgcatg cggatcgtct gtgtccccga
      121 ggtcttcgcc tccaccgtga acgtcacgat ctggtcaggt tcacgcaggg tcaccgtgcg
      181 ctcgttccca tcggggaact cgaactgcga cgaacgaagc tggatctgca cgatcaacgg
      241 ggtttcgcct gggtcgccca tgcgcagtgg tagctggccc tcgcccgacg tcagcgtgaa
      301 cgattgcgga tcggcgggca gcgcgcgagc gaagacgtgg tcggtgaagg cgttcacctg
      361 gtcgatccac cgcctgcccg cgagggggtc gtcgatgtag acgcccgcct cggcgtagag
      421 cagatcccta cggagccggt cgggggtggt cgagggttcg gccagcatcg atcggtaggc
      481 ctcgacgtcc cggcgctcgc tgcggatctt ctccgcgtag tcgtcttcga aacgagcctc
      541 ggacggtgcc gccaacgtgc tcgcgatctg caacgggttg acggtctcga cgaaggcttg
      601 ggggtgggtg ggttcgagga acggcgagtc ggcgagcctg cgcatcatgg gtgcccacat
      661 gccgggcggt agtgaggccg aaagcgccac ggcgacgcct cggacggtct cggtctggtt
      721 gggttggtcg gccggaaccg gttgttcgcg ccagatggta gcgagttcgc ccagcacggc
      781 ttgggctcct cggacggggt ccgatacgag gaccggatca gccaagagtg cctgcaccgc
      841 agggtcggga aggacgaggt cgatcgagga acccgacgcc agtgtgagac tggcggccgg
      901 gagcggcgcg aagtcattga gctgggcggt cctgtccacc gcatcggcgt tggccaggac
      961 cgcgcccacg cctcggatcg cgaggtcgtc catggtggca tcgtcgatcg cacctgccgg
     1021 cggccgttcg aacgatgtcg tgggctggcc cgctggcccg agcacctcaa cgaccgttcg
     1081 ctcgcccagg acgcgttgtc cctcgaggtc gaccgacaaa ccgccggcaa gcatcgccgg
     1141 catcaagggc gccgagaacg gcatggcaga gatctgcacg tcgggatcgg ccgccaccgt
     1201 ccgcaggtca cgcaccagag cggcagcatc ggtcgcaggc ttctcgtcgc gcccgatcac
     1261 cgaaccgtcg ccacgctcgt agccgtcgcc catacgaacg agctgatcga ggagcgccgg
     1321 ttctacggcc aggtcgatcg cgccgtggcc cggtgtctcg acgagtcgca ggagcgccgc
     1381 gacctgtgcg ccgagggtcc cttcgggcgt caccgatgtc tcgatcgatc gatcagtcag
     1441 ccgaccgtca ggcccgaacg cgataggtcc cgtgagttcg gaccaccagg aaaggagcat
     1501 gggttgttcc ggtgcttgga cgagatggat caacggggtg ttgagcgagg cctgcaccac
     1561 gccgtcggca cggatgtcga actgggcggg atagacggct gaatccgggg tctcgatgac
     1621 ccccgtcacc tggtcgacca tgttgaggtt gatgtcgaac aaccgttgtt ggccgggttc
     1681 gagcgagcct tcctgaggac gggccgtcgc gaaattgagg gtcggtccag gcccatcgat
     1741 cagggatcgc tcgtacacga accgggaatc gatctcggta ccgatcgaga tacccacgga
     1801 caggacgtcc agggccgtgt cgccggtgtt cgtggcctgc acggtgacct gcaggagtgg
     1861 ctcctcctgg gtcgtgatcg gcgtctggga gatcagcgtg agcgtgacgc cgggttcctg
     1921 ggccagtgcg ggacccggcc agggcagcat gcaggcaccc atcaccaagg acgaagccag
     1981 gagtgcgcgg aaggcgcggt agatgctcat gccagcgcgt ccatcgtagc gaccggggtt
     2041 cagcctcgga ggagcgcggc gagctcggtg gcgccgtaga gcaccgcgac caccaatagg
     2101 aacgagccca ccatcagccg caaggtccgt tccttcgtgt cgagggcgaa ccccgcgccg
     2161 aggcgggcgc cggggaccgc acccaacgtc acgacgagac agatggccca gtcgatgtgt
     2221 ccgagcatca cgtgcaccac cgtgcccggg accaccagcg cgacgatcgt gaggagcgag
     2281 gtgccgagcg cgaccttcaa cggcatgcca agccacccca ccatgagggg caccatgatg
     2341 aggccgcctc cgatgcccaa caatccactg acgaacccgg ctgcgagtcc gatcaccaag
     2401 aacatccagg gttcgggagc ccgggcgact tcagggtctt ctgccggtcg cgttcgggca
     2461 cctcgcagga tgccgatcgc ctgccaggcg aggagcaacg ccgtgaccac gagcaggacc
     2521 cgcgtgttca cgacatcggt cagcagcgcc ccggcgaccg cacccgcgat acccggtccc
     2581 accatccacg cggcggcccg cacgtccacc tgtcccgcac ggtgataggt ccacgcgccg
     2641 gtgagggccg tgggcaggat cgccgggagc ggcgtggcga gcgccacgat cggccgtgcg
     2701 cccatgagca cctggatgcc cggcgtcatc acgatgccgc cgcccacgcc gaagagtcca
     2761 ctgagcactc cggagacgaa accgaccgcg atgccgagca ccacgtccag tggggtcatc
     2821 gaagcaggtc ggcagcgcgg ccgagcacgt ctcgttcacc gcggtaggca gcacgcttga
     2881 gggcccgttc cagcgggaac cagcgcacct cttccatctc gtcgtcgcgg tcgtccgggt
     2941 cgcccccggt gcatcgcatc aagaagaagt ggaccgtctt gcggatgcgc gtgtcctccc
     3001 agacgtagaa gtacttggtc tcgccgagcg aggcttcgat ctcggccgta tacccggttt
     3061 cttccttgac ctcccgcaca gccgcctgtt cgtgcgtctc gccctcctcg atgccgccct
     3121 tcgcgagccc ccaggcgagt tcacccttgc gggttcgcct ggatgccagc acgacctcga
     3181 tctcgtctcc caggcgtcgg tagacgacac cgcctgccga gacttccttc tgcaccgggg
     3241 ctcgaggtcg tggcgctcgc ttcttggtct tcgacttcgg tttcggcttc ggtttcggct
     3301 gtgaggggtt cgtggccggg gggtcgccga gagcgacggg cgcagccgcc acggcagagg
     3361 cccccggctt acgacgacgt cgacgcctcc gtcgcttgcg cggcggcggt tcttggccgg
     3421 ggggtggctg ctcgtcgccc atcgcggcga ttctaggtgc cccggtcgcc cagaaggctc
     3481 aggtcggccc ccatggcgac cgatggtcag gcatggagat cgacgaactc ctccgtttcg
     3541 ccgtggaacg tgggtcgagt gaccttcacc tgaaggtcgg caacgtcccg ttcatccgcg
     3601 tggacggagc attgacgccg accgagttcg acgtgctgac gtctgccgat accgagacct
     3661 tcgcgcaggc gctgatgtcc gatcacaaga agcgggagtt cgaagcgacc agcgaggctg
     3721 acctgggcac gacgctgacc ggcgtcggcc gcttccgcgt caacgtgttc cgtcagcggg
     3781 gcgtggtggg gcttgcgatc cgtcgtgtcc gcagcgaggt gccgacgttc gatgaactgc
     3841 gactgccgcc ggtgatggag acgctggccg attccccgcg agggctcgtc ttgatcaccg
     3901 gccccaccgg gaccggcaag accaccacga tcgcgtcgat gatcggccac atcaaccg
//
LOCUS       DATDBZ010000568         3980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2015835, whole genome shotgun sequence.
ACCESSION   DATDBZ010000568 DATDBZ010000000
VERSION     DATDBZ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015835"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..546)
                     /locus_tag="VIX62_13090"
     CDS             complement(61..546)
                     /locus_tag="VIX62_13090"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5861210.1"
                     /translation="MTYELRLERLYDAPPEVVFDAFTDPANQAELHGEGTEGWAIQRA
                     ETDVRVGGTSTYSMGPEGVEPDIETRVFTVVDRPHRLVFRHSMKVAEWGGRSVDTEMT
                     LTFEDQDGKTLLTMVQTGFEREEDRDDFMGGWPDYLDTLKGVVGKQQDESLKARHDDD
                     D"
     gene            complement(543..884)
                     /locus_tag="VIX62_13095"
     CDS             complement(543..884)
                     /locus_tag="VIX62_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5861211.1"
                     /translation="MVQSQTLDRTFSALSDPRRRDILERLSFGPASISELARPLAISL
                     PGVMKHIRILEGAGLVTTEKKGRTRECRLEAGDMDEVSRWIEVYRLQWERRFDRLGQI
                     VERTKEGKGNR"
     gene            994..2148
                     /locus_tag="VIX62_13100"
     CDS             994..2148
                     /locus_tag="VIX62_13100"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY5861212.1"
                     /translation="MERRTWWERLRGINPLVVDGLLAATILGLSLFAATLGPEPVDRR
                     DPDAFAFLLIVLGCVPLMFRRRAALPVLWIVCASALAYAAFGYPDNLVVSLVLATYTA
                     ASLEPRDRVLTLVLPAAFAAGIATQLAAQTGFNWLEVVGGVTFSVAIPIAVGRAVFNR
                     RRRLREDRERMAHDAVVQERARIARELHDVVAHAMSVMIVQAGAARTVVERDPGEAAR
                     ALRRIEDTGRFGMAEMRRLIGVLKAGDEEAELMPQPGLAQLDQLLETVRSAGLPVEAV
                     TEGTPRPLPPGVELTAYRVVQEGLTNVLKHAGGAHARATVRYEEDAIAVEVADDGRGS
                     VVDPNGTPGHGLLGMRERVAVFDGTLETGARAGGGFVLRARIPLDIEVDG"
     gene            2145..2801
                     /locus_tag="VIX62_13105"
     CDS             2145..2801
                     /locus_tag="VIX62_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5861213.1"
                     /translation="MSTTVLIADDQALMRGGFRMIIDAEPDLEVVGEAIDGADAVRGF
                     ERLRPNVVVMDVRMPTMDGIEATSRITDAEPDAKILILTTFDLDEYVFQALRAGASGF
                     LLKDRPPEELVEAIRIVASGDALLAPSITRRLIEEFSRRPVAPAPPAELAELTDRERE
                     VLVLMARGLSNAEIAAQLFVAETTVKTHVGRVLHKLRLRDRAQAVVLAYESGLVQPGA
                     "
     gene            2930..3877
                     /locus_tag="VIX62_13110"
     CDS             2930..3877
                     /locus_tag="VIX62_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5861214.1"
                     /translation="MSTIEAGPGPADLRHDAPGSPAIVARGLTKRFRAVTAVDDLSFE
                     VRPGRVTGFLGPNGAGKSTTLRMLLALVKPTSGTATVLGLPYASLAQPTLAVGAVLEV
                     QSFHPLRTGRNHLRVLAAAAGLPDAKVAEVLDQVGLTDAADRKAGGYSLGMRQRLGLA
                     GALLGDPQVLILDEPANGLDPQGIRWLREFLQWFAGRGNAVLVSSHLLSEMALMADDV
                     IVIDHGRLIRQGTVAELTAGGSHLRVDSPDHDALGRALIAAGLEPRADDEGGFLLESA
                     EPERIGRIALQAGVALSRLSIEETSLEDAFLEMTEGIGR"
BASE COUNT          578 a         1331 c         1415 g          656 t
ORIGIN      
        1 cggacgcggt cgtccaggtg tgatcgcgtg atcgtgggtt gcgtcgccat ggttccctcc
       61 tcagtcgtcg tcgtcgtgcc gcgccttcag gctctcgtcc tgctgcttgc ccacgacacc
      121 cttgagcgtg tcgaggtagt cgggccaacc gcccatgaag tcgtcgcggt cctcctcgcg
      181 ctcgaacccg gtctgcacca tcgtgagcag cgtcttgccg tcctggtcct cgaaggtgag
      241 cgtcatctcg gtgtcgaccg atcgcccacc ccattcggcg accttcatgg agtgccggaa
      301 caccagccgg tgcggtcgat ccaccacggt gaacacgcgc gtctcgatgt cgggctcgac
      361 cccttcgggc cccatcgagt aggtggaggt gccgcccacg cgaacgtccg tctcggcccg
      421 ttggatcgcc catccctcgg tgccttcacc gtggagttcc gcttggttgg cggggtcggt
      481 gaacgcgtcg aagaccacct cgggtggcgc gtcgtagagg cgttccagtc gcagttcgta
      541 ggtcatcggt tccccttccc ttccttcgtg cgttcgacga tctggcccag ccggtcgaaa
      601 cgacgctccc actgcagccg gtacacctcg atccagcgcg aaacctcatc catatccccg
      661 gcttcgagac ggcattcacg agtgcggccc tttttctcgg tggtcacgag cccggctcct
      721 tcgaggatgc ggatgtgctt catgacgccg ggcagcgaga tggcaagcgg gcgagcgagt
      781 tcgctgatcg atgccggccc gaacgacagg cgctcgagga tgtcgcggcg cctcgggtcc
      841 gagagcgccg agaacgtccg gtcgagggtc tgagactgaa ccatctggtt aagtatcgcc
      901 gtcgagggcc gaagcgtcaa cgggtcatcg cgtcccgccg aatcgttcct cgggatgacg
      961 ccgggcgctc ggcggaccgt taccgtccag gtcgtggaac gacgcacgtg gtgggagcgc
     1021 ctgcgcggga tcaacccgtt ggtggtggac ggcctcctcg ccgcgacgat cctggggctc
     1081 tccctcttcg cagccaccct cggtcccgag ccggtcgacc gccgtgatcc agacgccttt
     1141 gcgttcctcc tgatcgttct cggatgcgtg ccgctcatgt tccgccgtcg ggccgctctg
     1201 ccggtcctat ggatcgtgtg tgcatcggcg ctcgcctatg cagcgttcgg ttaccccgac
     1261 aacctggtcg tgtcgctggt gctggcgacc tacacggccg cctcgctcga accccgcgat
     1321 cgggtcctca cgttggtgct acctgctgcg ttcgcagccg ggatcgcgac gcagctcgcc
     1381 gcgcagaccg gcttcaattg gctcgaggtc gtgggcggcg tgaccttctc ggtggcgatc
     1441 cccatcgccg tcggtcgcgc ggtcttcaac cgtcggcggc gcctgcgcga ggaccgggaa
     1501 cgcatggctc acgacgccgt cgtgcaggaa cgggcccgca tcgcccgtga gctgcacgac
     1561 gtcgtcgcgc acgcgatgag cgtgatgatc gtccaggccg gagccgccag gaccgtcgtc
     1621 gagcgcgatc ccggtgaggc agcgcgggcc cttcgtcgga tcgaagacac cggccggttc
     1681 ggcatggccg agatgcgccg gctgatcgga gtgttgaaag ccggagatga ggaagccgag
     1741 ctgatgcccc agccgggact cgcacagctc gaccagctgc tcgagaccgt tcggagcgcc
     1801 ggcttgcccg tcgaggccgt gaccgagggc acaccccggc cgctgcctcc cggtgtcgag
     1861 ctcaccgcct accgcgtcgt acaggaggga ttgacgaacg tgctgaagca cgccggcggt
     1921 gcgcatgccc gcgccacggt gcgctacgag gaggacgcga tcgcggtgga ggtggccgac
     1981 gacgggcgcg ggtcggtggt cgacccgaac ggcacgcccg gccacggcct gctcggcatg
     2041 cgcgagcggg tcgcggtgtt cgacggcacg ctcgagaccg gtgccagagc gggcggcggc
     2101 ttcgtgctgc gcgctcggat cccgctcgac atcgaggtgg atggatgagc accacggtgt
     2161 tgatcgcgga cgaccaggcc ctgatgcggg gtgggttccg catgatcatc gacgccgaac
     2221 ccgatctcga ggtggtgggc gaggcgatcg acggcgccga cgccgtgcgc gggttcgagc
     2281 ggctgcggcc gaacgtggtc gtcatggacg tgcggatgcc cacgatggac gggatcgagg
     2341 ccaccagccg gatcaccgac gcggagcccg atgcgaagat cctgatcctc accacgttcg
     2401 atctggacga gtacgtgttc caggcgctgc gtgcgggtgc gagcgggttc ctgctgaagg
     2461 accgtccgcc cgaggaactg gtggaggcga tccggatcgt ggcgagcggc gacgcgctgt
     2521 tggctccatc gatcaccagg aggctcatcg aggagttctc ccggcgaccc gtggcgcccg
     2581 ctccacccgc cgagctggcc gagctcaccg accgcgaacg ggaggtgctg gtgctgatgg
     2641 cgcgaggact gtcgaacgcc gagatcgccg cccagctctt cgtcgccgag accacggtga
     2701 agacccacgt gggccgggtg ttgcacaagc tccggttgcg tgaccgcgcg caggccgtcg
     2761 tcctggcgta cgaatccggc ctggtccaac cgggcgcgta gtcggcccgc cgccaccggc
     2821 gtcctcctgg gggatgacct ccgaacggta ccgggggccg acgcggcccc cggccacccg
     2881 ctcctagcgt ggaacccgac gggaagtcca cgtgaaggag aggacgatga tgagcaccat
     2941 cgaggcgggt cccggaccgg cggaccttcg gcatgacgca ccgggatcgc cggccatcgt
     3001 tgcgagggga ctcacgaagc gcttccgcgc ggtcacggcc gtcgacgacc tcagcttcga
     3061 ggttcgaccg ggtcgggtga ccgggttcct gggaccgaac ggcgccggga agagcacgac
     3121 gttgcggatg ctcctggcct tggtgaagcc cacgtccggc acggccaccg tgctgggtct
     3181 gccgtacgcg agcctggccc agcccaccct cgccgtcggc gccgtgctgg aggtccagag
     3241 cttccatccg ctgcgcaccg ggcggaacca cctccgggtg ctcgctgccg ccgcggggtt
     3301 gcccgatgcg aaggtcgccg aggtgttgga ccaggtgggc ctgaccgatg cggccgaccg
     3361 caaggccggg gggtactcgc tcggcatgcg gcagcgcctc gggttggccg gcgccctgct
     3421 cggcgatcca caggtgctga tcctggacga accggcgaac ggcctggacc cccaaggcat
     3481 ccggtggctg cgtgagttcc tgcagtggtt cgcgggtcgg ggcaacgcgg tgctggtgtc
     3541 gagtcacctg ttgtcggaga tggcgctgat ggccgacgac gtgatcgtga tcgaccacgg
     3601 acggctgatc cgccaaggca cggtggccga gctcacggcc ggcggttcgc acctgcgggt
     3661 cgactcgccc gaccacgacg cgctgggtcg cgccctgatc gccgccggcc tggaaccacg
     3721 ggccgacgac gaggggggct tcctgctcga gtcagccgaa cccgagcgga tcgggcggat
     3781 cgcactccaa gcaggggtgg cgctgtcacg gctgagcatc gaggagacga gcctcgagga
     3841 cgcgttcctc gagatgacgg aagggatcgg acgatgaggt acgcgatgag gtcggagttc
     3901 cgaaagctga tgaccacccg aaccgtgtgg ggtctgctcg ccggggtcgt ggtcttgacc
     3961 gggctgggcg cctggggaac
//
LOCUS       DATDBZ010000569         2597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_176558, whole genome shotgun sequence.
ACCESSION   DATDBZ010000569 DATDBZ010000000
VERSION     DATDBZ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176558"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VIX62_13115"
     CDS             <1..468
                     /locus_tag="VIX62_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5861215.1"
                     /translation="GVEDLTLELTIPSFYSTTIPQILVSDLNEVGITLQVNSVDFPTW
                     LNDVYINKDYDLSFVLHTEAHDFENWANPEYYFTYDNPEVQDLYAQSLAATDEAEAAD
                     LLAQAARIVSEDSAADWLYNGASVVAVGSNITGFPSVNVNERLNLAELAKSNG"
     gene            465..1415
                     /locus_tag="VIX62_13120"
     CDS             465..1415
                     /locus_tag="VIX62_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5861216.1"
                     /translation="MIRYTLTRLALLLLGLLVASVLIFVTLRVLPGDIAQLIAGTNST
                     PEQIEAIRERLGLNQPLPLQYLEWIGGIFRGDLGASLLTGSSVTSELAQKAQVTVPLG
                     IMSLTIAILFSIPLGVLSAMRRGRGDGTALSVGAQALAAVPVVWAGMMFVVVFAVWLG
                     WLPAQGFPRAGWNDPAAAFRSLLLPALTIGIVEGAMLMRFVRSATMQAVGQDYVRTAA
                     AKGLTRNAALIRHGLPNVGLSVITVLGLQVAGIIVGAVVIEQLFSLPGIGRMLVADVS
                     ARDLPKVQGELLVLTGFVLVIGFVVDLVHRVIDPRQRETT"
     gene            1412..2362
                     /locus_tag="VIX62_13125"
     CDS             1412..2362
                     /locus_tag="VIX62_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5861217.1"
                     /translation="MSAASARAEAADRKSAARRRRWTWLRRLWSLSTGRFGLIVVAVV
                     TATAIIARFWTPFDPRQVAIESRWALPGWPHLLGTDGTGRDILSLLMAGSRTTVLVAV
                     GAGVIATVIGVALAALGALTARWLRESVAVLVDILIAFPVLIIAMMISAVWGGSLAVV
                     IWSVGIGFGVNIARVTRPELRRVLHSDFVLAGRASGLTPLQNLWRHLMPNVAPVFIVQ
                     LSWGMAVAVLAEAGLSYLGFGAPVTEPSWGLLLAELQQYITVYPLSVLWPGLAITFTV
                     LGLNLLGDALREATDPTLSRGRDPLHPPRGAIRHVPEVVA"
     gene            2359..>2597
                     /locus_tag="VIX62_13130"
     CDS             2359..>2597
                     /locus_tag="VIX62_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5861218.1"
                     /translation="MTLRVTDLTVDIGERRVVDGVSFEVPDGARVGLIGESGSGKSLT
                     ALAILGLLPQGAIASGSVRWNGRELIGLPDRELATL"
BASE COUNT          359 a          925 c          869 g          444 t
ORIGIN      
        1 ggcgtcgaag acctcactct cgagctgacg atcccgagct tctactccac cacgattccg
       61 cagatcctcg tctcggacct caatgaggtg ggcatcacgc tccaggtcaa ctcggtcgac
      121 ttccccacgt ggctgaacga cgtctacatc aacaaggact acgacctgag cttcgtactg
      181 cacaccgagg cgcacgactt cgagaactgg gccaacccgg agtactactt cacgtacgac
      241 aaccccgagg tccaggacct gtacgcgcaa tcgctcgccg cgaccgacga ggccgaagca
      301 gccgacctgc tcgcgcaggc cgctcgcatc gtgtcggagg actccgccgc cgactggctc
      361 tacaacggag cctccgtcgt ggccgtcggc tcgaacatca ccggcttccc ctcggtcaac
      421 gtgaacgagc gcctcaacct cgcagagctc gccaagagca acgggtgatc cgttacacgc
      481 tgacacgatt ggccctgctt ctgctcgggc ttctcgtggc cagcgtgctg atcttcgtca
      541 ccctgcgcgt gctgccgggc gacatcgcac agctgatcgc cggtacgaac agcactcccg
      601 agcagatcga ggcgatccgg gagcggctcg gactgaatca gccgctccca ctccagtacc
      661 tggagtggat cggcggcatc ttccgcggcg acctcggcgc ttcgctgctc accggatcga
      721 gcgtgacctc cgagctggcg cagaaggcgc aggtgaccgt gccgctcggg atcatgtccc
      781 tgacgatcgc gatcctgttc agcatccccc tcggcgtcct ctcggcgatg cgccgcggcc
      841 gcggcgacgg cacggcgctg agcgtcggtg cgcaggcact ggccgccgta ccggtcgtct
      901 gggcggggat gatgttcgtc gtcgtcttcg ccgtgtggct gggctggctg cccgctcaag
      961 gcttcccgag ggcaggctgg aacgatccgg ccgccgcgtt ccgctccctg ctgctgcccg
     1021 ccctcacgat cggcatcgtc gagggcgcga tgctcatgcg cttcgtgcgc agcgcgacga
     1081 tgcaggcggt cggccaggac tacgtccgca ccgcggccgc gaagggactc acgcgcaacg
     1141 ccgcgctcat ccgccacggg ctgcccaacg tcggcctctc ggtgatcacg gtgctggggc
     1201 tgcaggtcgc gggcatcatc gtcggagctg tcgtgatcga gcagctcttc tccctccccg
     1261 ggatcgggcg gatgctggtc gccgacgtga gcgcgcgcga ccttcccaag gtgcagggtg
     1321 agcttctggt gctcaccggc ttcgtgctcg tgatcggctt cgtcgtcgat ctggtgcacc
     1381 gggtgatcga ccctcgccaa cgggagacga cgtgagcgcg gcgagcgccc gggcggaggc
     1441 cgccgaccgg aagagcgcgg cgcggcgtcg acgctggacg tggctgcgac gcctgtggag
     1501 cctgtccacc ggccggttcg gcctcatcgt cgtggcggtc gtcacggcga cggcgatcat
     1561 cgcgcgcttc tggacgccct tcgacccgcg gcaggtggcg atcgagagcc ggtgggccct
     1621 ccccggctgg ccgcatctgc tcggcacgga cggcaccgga cgcgacatcc tcagcctcct
     1681 catggcgggc tcgcgcacga ccgtgctcgt cgccgtcggg gcgggcgtga tcgccaccgt
     1741 catcggagtg gccctggctg cgctcggcgc gctcaccgcg cggtggctgc gagaatcggt
     1801 cgccgtgctg gtcgacatcc tgatcgcctt tccggtgctg atcatcgcga tgatgatcag
     1861 cgcggtgtgg ggcggttcgc tcgccgtcgt gatctggtcg gtcggcatcg ggttcggcgt
     1921 gaacatcgca cgcgtcacca gacccgagct gcgccgtgtg ctgcacagcg acttcgtgct
     1981 ggccggccga gcctctgggc tcaccccgct gcagaacctc tggcgccacc tgatgccgaa
     2041 tgtcgcgccg gtgttcatcg tgcagctctc atggggaatg gcggtcgcgg tcctcgccga
     2101 ggcggggctg tcctacctcg ggttcggcgc gcccgtcacc gagccgtcgt gggggctgct
     2161 gctggcagaa ctgcagcagt acatcacggt ctacccgctc tcggtgctgt ggcccggcct
     2221 ggcgatcacg ttcacggtgc tcggcctgaa cctgctcggc gatgcactcc gtgaggcgac
     2281 cgacccgacg ctgtcgcgcg gccgcgaccc tctgcatccg ccccgcggcg cgatccggca
     2341 cgtgccggag gtggtcgcat gaccctgaga gtcaccgacc tcacggtcga catcggcgag
     2401 cgccgcgtcg tcgacggagt ctcgttcgag gtgcccgacg gcgcccgcgt cggcctgatc
     2461 ggcgagtcgg gatcgggcaa gtccctcacc gccctcgcga tcctcgggct ccttccccag
     2521 ggcgcgatcg cctcgggaag cgtgcgctgg aacggacggg agctcatcgg cctgcccgat
     2581 cgcgagctcg ccacgct
//
LOCUS       DATDBZ010000570         2853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1007031, whole genome shotgun sequence.
ACCESSION   DATDBZ010000570 DATDBZ010000000
VERSION     DATDBZ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007031"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VIX62_13135"
     CDS             complement(<1..463)
                     /locus_tag="VIX62_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861219.1"
                     /translation="MAAPSDDPVVAGRGALERHAWTEAYEVLTRADGEHALGGEGLTL
                     LAAAAYWTAHPDETVEYLERAYGAYVEEGDPASASMIAFRVAEQHGMRTELPQAQGWA
                     ARAMHLAEDDPAWPVHGWLAWMQGLLAWFSAEYEQAIGAYDRAIELASASRD"
     gene            complement(534..1973)
                     /locus_tag="VIX62_13140"
     CDS             complement(534..1973)
                     /locus_tag="VIX62_13140"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY5861220.1"
                     /translation="MKKLHLAGALLLFCAFCAATIVLVPTTARPAPSSPNIVVIMTDD
                     QRWDTLSRMPNVQHLLQQQGTTYTNGFVPNSLCCPSRATTLTGLNARTTGVYSNTPPF
                     GGFASFKDQTTIATVLRGQAGYRTMFVGKYLNGYLGGRYGYVPPGWDRWFSIAGGRYY
                     SYYAADNGRKSPLYGTGPEAYSGRVLTTKAIDYVTTAPTTSPFFLFYAPVAPHAAQNQ
                     AYAIPDPRDVGVLETLPGARPPSYGFKDSVADMPAYIQANGWNVNGVDRFRRRQLESV
                     IGVDRNIGELMAVLPANTLVIFMSDNGFMWAEHKWHYKSAPYEESIRIPLVVRWDGVV
                     AAGATDARLALNVDIAPTIATAAGIDVSTTPLGLDRNGVPRLTEGLNLLGASTRSSFV
                     LEHYENQSKVPPYCGIRTEDGWMYTRYWDGRTTIADNGFEELYHVAVDPYQLTNLVTD
                     PSSADTLSTLRALTRERCSPAPPYYVWPT"
     gene            2173..2691
                     /locus_tag="VIX62_13145"
     CDS             2173..2691
                     /locus_tag="VIX62_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861221.1"
                     /translation="MHRTIGPLVSAVILGVLLCMPSATAVVLDRDTGFDPDDVDSPSR
                     FDPDIRSSTRKVSTRDGRRVLAIIVRFYERDAGWGLGVRLDARGGHRADHLMSTLGQH
                     CYVWPSGSRGEKVEGRASAHGERFVCKVPVRVISPNKPIRWKIHTHVPDGSRLSRTDF
                     EIDYAPNQGWYS"
BASE COUNT          453 a          915 c          981 g          504 t
ORIGIN      
        1 gatccctcga cgccgaggcg agttcgatgg cccggtcgta ggcgccgatc gcctgctcgt
       61 attccgcaga gaaccacgcg aggaggccct gcatccacgc gagccacccg tgcacaggcc
      121 acgccgggtc gtcctccgcc aggtgcatcg ctctcgccgc ccacccttgt gcttgcggga
      181 gttccgtcct catgccgtgc tgctccgcga cgcggaaggc gatcatggag gccgacgcgg
      241 ggtcaccctc ctccacatac gcgccgtagg cgcgctccag gtactcgacg gtctcgtcgg
      301 gatgggcggt ccagtaggcg gcggcggcga ggagcgtgag cccttcaccg ccgagcgcat
      361 gttcaccgtc ggcgcgggtc aagacctcgt aggcctccgt ccacgcatgt cgctcgagtg
      421 cgccccgccc cgcgacgacc gggtcgtcgg atggtgcagc cacgtccgaa tcctacgccg
      481 atgtccgttc cggagcggag cgtgggagtg ccctcacccg gcgtccgcga cggctaggtc
      541 ggccacacgt agtacggcgg agcgggactg catcgttctc gcgtaagggc gcgcagcgtc
      601 gacagggtgt cggccgagct cggatcggtg acgaggttcg tgagttgata cggatccacc
      661 gccacgtggt agagctcctc gaagccgttg tccgcgatcg tggtcctgcc gtcccagtac
      721 cgcgtgtaca tccacccgtc ctcggtccgg atcccgcagt agggcggcac cttgctctgg
      781 ttctcgtagt gctcgaggac gaacgatgat cgcgtggatg cgcccaacag gttgagccct
      841 tcggtcagcc ggggaacacc gttgcgatcg agcccgagcg gggtcgtcga gacatcgatt
      901 ccggctgccg tcgcgatcgt cggggcgatg tcgacgttca gcgccaagcg cgcgtccgtg
      961 gcccctgcgg ccacgacgcc gtcccatcga acgacgagcg ggatgcggat cgattcctcg
     1021 tagggagcgc tcttgtagtg ccacttgtgt tcggcccaca tgaagccgtt gtccgacatg
     1081 aagatcacca acgtgttcgc ggggagcacg gccatcaact cgccgatgtt ccggtcgacg
     1141 ccgatcacgg attcgagctg cctgcgccgg aaccggtcca ccccgttcac gttccagccg
     1201 ttggcttgga tgtaggcggg catgtccgcg accgagtcct tgaaaccgta ggacgggggg
     1261 cgggcgcccg gcagggtctc caggacgccg acgtcccgag gatcggggat cgcgtaggcc
     1321 tgattctggg cagcgtgcgg ggcgacgggc gcgtagaaca ggaagaacgg cgacgtcgtc
     1381 ggagcggtgg tcacgtagtc gatcgccttc gtggtgagga cccgtcccga gtacgcctcg
     1441 gggccggtgc cgtacagcgg tgatttccgt ccgttgtccg ccgcgtagta gctgtagtac
     1501 ctgccaccgg cgatcgagaa ccaacggtcc caccccggag gcacgtagcc gtagcgccct
     1561 cccaggtagc cgttcaggta tttgcccacg aacatcgtcc ggtacccggc ctgcccgcgg
     1621 agcaccgtgg cgatcgtcgt ctggtccttg aaggaggcga agccgccgaa cggcggggtg
     1681 ttcgagtaca ccccggtcgt gcgtgcgttg agcccggtca aggtcgtggc ccggctgggg
     1741 caacacaacg agttcggaac gaacccgttc gtgtaggtgg tcccttgctg ctgcaggagg
     1801 tgctggacat tcggcatgcg cgagagcgtg tcccagcgct ggtcgtcggt catgatcacg
     1861 acgatgttgg gcgaactggg tgccggccgt gccgtcgtgg gcaccaagac gatggttgcc
     1921 gcgcagaatg cgcagaacag gagtagggca cctgctaggt ggagtttctt catgatggca
     1981 tcgtgtgccg gagcaacccc ccttgccatc cctcgctcac cgtagggcgg gagcccggca
     2041 gcccgcccga gggtgtaacc gaacgattac ggtggacgcc tgatcacgtc gccggttcga
     2101 gccgatgccg cttgagttcg gaatggaggc cggattaggg tggcggtgcc cgctttgcgg
     2161 aaggggtcct tcatgcatcg gacgatcgga ccgttggtgt cggccgtcat cctgggcgtc
     2221 ttgttgtgca tgccatcggc gacggcggtt gtgctcgacc gggatacagg cttcgacccc
     2281 gacgatgtcg attcaccctc tcgcttcgac cccgacatca ggtcgtcgac gcgcaaggtc
     2341 tcaacgcgag atgggcgccg tgtcctcgcg atcatcgttc gcttctacga acgtgacgcg
     2401 ggttgggggt tgggggtgcg cctggatgcc cggggcggac atcgcgccga ccacctcatg
     2461 agcaccctcg gccaacactg ctacgtgtgg ccctcgggtt cccgcggcga aaaggtggaa
     2521 ggtcgcgcct ccgcacacgg cgagcgattc gtctgcaagg tgccggttcg ggtcatctcc
     2581 ccgaacaagc cgatccgctg gaagatccac acgcatgtgc ccgatggctc caggttgagt
     2641 cgaaccgact tcgagatcga ctacgcgccg aaccagggct ggtacagctg agcgcgttgg
     2701 ctcgcgcgag ggagggtcct gtttgtcgcg acctcggggg cacctcgaga gttgaggtcg
     2761 catcggcaga cgaagcaggc ctcgatctca cgatcgcgat catcccccga gacgggcgct
     2821 gacgtgatca ggcagcacga aggccgggcc aac
//
LOCUS       DATDBZ010000571         3764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2649608, whole genome shotgun sequence.
ACCESSION   DATDBZ010000571 DATDBZ010000000
VERSION     DATDBZ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2649608"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VIX62_13150"
     CDS             complement(<1..717)
                     /locus_tag="VIX62_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5861222.1"
                     /translation="MSKIRSIEVRALAPDVERFRYTAFQDEVFTTTTLVRIEDQDGAS
                     GIGAYDSDSYGDWDRAPLETLRTLVPTLVGMDALDHDAVAALLTEDGCAPVPPAVRST
                     SDIALWDLAARAAAQPLHVALGGDPASTSLPSYASVALMDDKAAYLEAIDGWVDRGFG
                     AVKLHAWGDPERDASLITAVRGRFPSLTLMHDAECRYTHEGAERVARACADAGVRWFE
                     APLPDFDLQGYRDLRRQVPAV"
     gene            800..1498
                     /locus_tag="VIX62_13155"
     CDS             800..1498
                     /locus_tag="VIX62_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5861223.1"
                     /translation="MSESATSAPLQPLQAPRSLAEDAADRIREQILAGGFRQGEHLVE
                     AKIAEQLNISRGPVREAFKLLRAEGLLNEEPRRGTFVVSLSADDVREIYGLRAALEGR
                     AARLIARAHDPATIEQLRTLADAIDAAVATGDGAAVSRADLAFHEGLCALCGNSRILE
                     VFDRYVPTLRALLRLDERVLRSLDEVSNQHRPFVEAIEAGEEETAARLLAEHAEHAGE
                     LIAGVVAANEAAPD"
     gene            1627..2733
                     /locus_tag="VIX62_13160"
     CDS             1627..2733
                     /locus_tag="VIX62_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5861224.1"
                     /translation="MSRFLRGASVLVALTLVAAACGDGDPEPSAGTGGSPGDPASITG
                     TVRLYSYSDGFDPDYMESFYETYPNIELETASFSSNEEAVAKIQAGFEADVVNSCVDE
                     ATLEMVNKGMYMPLDTARLENWDDIWPGMKELPGVQVDGQAYIVPVDAGTAGIMYNAD
                     VITTPPTSWKDLFDPQYAGRASLEDLAITALDVAALANGIENPLEMTDEELQMVTQYL
                     IDHRDQFRTFYKGQADIRAQFKSGEVIISSGYPGDAKLMRRDGTNVQFAAAEEGQMLW
                     TCGYGISPDIADENLDAAYALLNWYTSLPPQIYAATNFNYLTSNQGIIDAVPPKVVEE
                     AALDSLFTLDNAIPASPPDNREAWIAAWTEVKAS"
     gene            2733..3647
                     /locus_tag="VIX62_13165"
     CDS             2733..3647
                     /locus_tag="VIX62_13165"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5861225.1"
                     /translation="MVQAVGERNGLAVTDVEGASRARRRRAANAWGWRLFLAIVMLAL
                     FGPLLILVLFSFNDSSVLAFPFEGFTLHWYSDALSDPTLREAIANSFVVAFVVAPLCL
                     VLGTLAAFGLTRFRFRGRGAIGGLVGAPLVLPWLIIGIAALMAYARVDVNLNLRTVIA
                     TQTVCTFPLVTAIVAAQLFRFQKVQEEAAIDLGCSRLQVLRHIILPHIFPALAAAAIF
                     AFTWSFNNYEISAFTLGFQQTFPVWVYSSVRNPDHTAVVNAISTIISVGQIAIIWFAL
                     RLLRSRSGDEGTKSLIAGTEAIAAGAKT"
BASE COUNT          592 a         1290 c         1278 g          604 t
ORIGIN      
        1 cacggcgggc acctgccggc gcaggtcgcg gtagccctgc agatcgaagt cgggcagcgg
       61 cgcctcgaac cagcggacgc cggcgtcggc gcaggctcga gcgacccgtt cggcgccctc
      121 gtgggtgtat cggcactccg cgtcatgcat cagcgtgagc gaggggaagc gtccccggac
      181 ggccgtgatc agggaggcat cgcgctcggg atcgccccac gcgtggagtt tcacggcgcc
      241 gaaccctcgg tccacccacc cgtcgatcgc ctcgaggtag gcggccttgt cgtccatcag
      301 cgcgaccgat gcgtaggacg ggagcgacgt ggacgcgggg tcgcctccca gggccacgtg
      361 gagcggctgg gccgccgcgc gcgcggccag gtcccagagg gcgatgtcgc tcgtggagcg
      421 caccgcgggc gggaccggtg cacagccgtc ttcggtgagc agtgcggcca cggcgtcgtg
      481 gtcgagcgcg tccatcccca ccaaggtggg gaccagcgtt cggagggttt ccagcggcgc
      541 gcggtcccag tcgccgtagg agtcggagtc gtaggcgccg atcccgctcg cgccgtcctg
      601 gtcctcgatc ctgaccaggg tggtcgtggt gaacacctcg tcctggaacg cggtgtaccg
      661 gaaccgttcc acgtcgggag ccagcgcgcg gacctcgatc gagcggatct tcgacacccg
      721 cgcgctcctc cttcccgagg cccttgggaa ccccgacgtc atcggtcaac agttgacaac
      781 cgcatgctag ggtacgcgca tgagcgaatc cgcgacgtcg gcgccgctgc aaccgttgca
      841 ggcgcctcgt tccctcgccg aagatgccgc cgaccggatc cgagagcaga tcctggcggg
      901 cgggttccgg cagggcgagc acctggtcga agccaagatc gccgagcagc tcaacatcag
      961 ccgtggaccg gtgcgcgagg cgttcaagct cctccgggcc gagggattgc tcaacgagga
     1021 accacgccgg gggaccttcg tggtcagcct cagcgccgac gacgtccgcg agatctatgg
     1081 cctgcgcgcc gcgttggagg gccgagcggc gcgattgatc gcccgcgccc atgaccccgc
     1141 gacgatcgag cagctgcgca ccctcgccga cgcgatcgat gcggccgtcg ccacggggga
     1201 cggcgccgcg gtgtcgcgcg ccgatctggc cttccacgaa gggctctgcg cgctctgcgg
     1261 caactcccgg atcctcgagg tcttcgatcg ctacgtgccc acgctccgcg cgctgctccg
     1321 tctcgacgag cgggtcctgc gctcgttgga cgaggtttcg aaccagcacc ggcccttcgt
     1381 cgaggccatc gaggcgggcg aagaggaaac cgccgctcgc ctgctcgccg agcacgccga
     1441 gcacgccggt gagctgatcg ccggcgtcgt ggccgcgaac gaagcggccc ccgactagcc
     1501 cgttcggccc tgctccaacc tcgccatttt gtcgacggtt gacaatctac ctgccttgac
     1561 ctaggttcgg cgcgtggttg aaggtcagag cttcgaaacc acacgaaccc acggggaggg
     1621 tccttgatgt cacggttcct acgaggcgca tcggtgctgg tcgcgctgac cttggtcgcg
     1681 gcggcgtgcg gcgacggtga tcccgagccg tcggcaggga ccggtgggtc accaggcgat
     1741 ccggcgtcca tcaccggaac ggtgcgcctg tactcctatt ccgacgggtt cgaccccgac
     1801 tacatggagt cgttctacga gacctacccc aacatcgagc tcgagacggc ctcgttcagc
     1861 tcgaacgaag aggccgtcgc gaagatccag gctgggttcg aagccgacgt cgtcaacagc
     1921 tgcgtggacg aagcgacgct cgagatggtg aacaagggca tgtacatgcc actcgacacc
     1981 gctcggctgg agaactggga cgacatctgg cccgggatga aggaactgcc cggggttcag
     2041 gtcgacggcc aggcctacat cgtgccggtc gacgccggca ccgcggggat catgtacaac
     2101 gccgacgtca tcaccacgcc gcccacctcg tggaaggacc tgttcgaccc gcagtacgcc
     2161 ggccgggcct cgctcgagga cctcgccatc accgccctcg acgtggccgc gctcgccaac
     2221 gggatcgaga acccgctgga gatgaccgac gaggaactcc agatggtgac ccagtacctc
     2281 atcgaccacc gcgaccagtt ccgcaccttc tacaagggcc aggccgacat ccgcgcccag
     2341 ttcaagtcgg gcgaggtcat catctcctcg ggctatcccg gcgacgcgaa gctgatgcgg
     2401 cgcgacggca cgaacgtgca gttcgccgcc gccgaagagg gccagatgct gtggacctgc
     2461 ggatacggca tcagccccga catcgccgac gagaacctgg acgccgccta cgcgttgctc
     2521 aactggtaca cgtcgttgcc gccgcagatc tacgcggcca ccaacttcaa ctacctgaca
     2581 tcgaaccagg gcatcatcga cgcggtgccg ccgaaggtgg tcgaggaagc cgcgctggat
     2641 tcgctgttca ccttggacaa cgcgatcccg gcgagcccgc cggacaaccg tgaggcgtgg
     2701 atcgcggcct ggaccgaggt caaggcgtcg tagtggtcca ggcggtcggg gagcggaacg
     2761 gcctggcggt caccgacgtc gaaggtgcct cccgggcacg gcggcgccgc gcggccaacg
     2821 cctggggctg gcggttgttc ctcgccatcg tgatgctcgc gctgttcggc ccgctcctga
     2881 tcctggtgct gttctcgttc aacgactcga gcgtattggc gttcccgttc gagggcttca
     2941 ccctgcactg gtactccgat gcgttgtccg accccaccct ccgggaggcg atcgccaact
     3001 cgttcgtggt ggccttcgtg gtcgccccgc tctgcctggt gctgggcacc ttggcggcgt
     3061 tcggactgac ccggttccgc ttccgggggc gaggggcgat cggcgggttg gtgggcgcgc
     3121 cgttggtgct gccgtggctg atcatcggga tcgcggccct catggcctac gcgcgcgtcg
     3181 acgtgaacct caaccttcgc accgtgatcg ccacccagac ggtctgcacg ttcccgctgg
     3241 tgacggccat cgtggcggcg cagctgttcc ggttccagaa ggtgcaggaa gaggcggcga
     3301 tcgacctggg atgctcgcgc ctgcaggtgc tgcgccacat catcctgccg cacatcttcc
     3361 cggcgctcgc cgcggcggcg atcttcgcgt tcacctggtc gttcaacaac tacgagatca
     3421 gcgcgttcac cctgggcttc cagcagacgt tcccggtgtg ggtgtactcg tcggttcgca
     3481 accccgatca caccgcggtg gtgaacgcca tctccacgat catctcggtc gggcagatcg
     3541 ccatcatctg gttcgcgctg cggctgctcc gctcccgttc gggcgatgaa ggcaccaaga
     3601 gcctgatcgc gggcaccgaa gcgatcgcgg cgggagcgaa gacctgatgg cgagcgtcgg
     3661 cgaggtcctg gagatcccga agggttcgga gggcaagcgt ccgaggggct caggtacgcg
     3721 acagagccgg ggggcgctgg gcatcctgtt cgcgccggtc accc
//
LOCUS       DATDBZ010000572         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_3051227, whole genome shotgun sequence.
ACCESSION   DATDBZ010000572 DATDBZ010000000
VERSION     DATDBZ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3051227"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VIX62_13170"
     CDS             complement(<1..864)
                     /locus_tag="VIX62_13170"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY5861226.1"
                     /translation="MRLGIITGGGDCPGLNAVIRAAVRKGVGVHGHTFMGFRDGWRGV
                     VDDDSIGLGIPQITGILHRGGTILGSSGMNPFRVDGGPEAVEATFERRGLDGLIAIGG
                     EGTLGAAAAFADRGLPVVGVPKTIDNDLAGTDITVGFHTAVQVATDAIDRLHSTAESH
                     DRVMLVEVMGRHAGWIAVYAGIAGGADVILIPEHPFDIEDVCRMLRHRHMHRSSFSIV
                     VVAEGAIPQEGTLVLPEPEVDENGFPRLGGVANHIAPEIEKRTGFQTRVTILGHVQRG
                     GSPVAFDRVLAT"
     gene            complement(917..1192)
                     /locus_tag="VIX62_13175"
     CDS             complement(917..1192)
                     /locus_tag="VIX62_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861227.1"
                     /translation="MPRTRYPTFGIKRSACRPDDRWVYEKDDAGADMSLGDKQAIDRS
                     TDDHLGFGGSAKATDEDARQDVQTEDDEASYVDSWYQVLKAQAVDEA"
     gene            1228..1389
                     /locus_tag="VIX62_13180"
     CDS             1228..1389
                     /locus_tag="VIX62_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861228.1"
                     /translation="MVMAGLFPDVSNWGFLEYFFVGLLLLLVALVTVFAGYVFLQQFR
                     NPARRGSRP"
     gene            complement(1386..>2123)
                     /locus_tag="VIX62_13185"
     CDS             complement(1386..>2123)
                     /locus_tag="VIX62_13185"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEY5861229.1"
                     /translation="REVVHPMPLDVCGAESQGQIGYLLQVTIGDVFFERGMERPVATV
                     LTLTRVRPDDPAFTDPTKFVGPFYEPEEARALESERGYVMKADPHGGWRRVVPSPSPY
                     SIVETPVIRQLVAAGTIVIASGGGGVPVIEDGPRLIGKEGVVDKDRAAAILAKEVEAS
                     TLLILTDVKKVQRGYGSFMPEDIDRMTVREATQLLRKGEFGAGSMGPKVEAAATFARG
                     GGRSVIADLADATAALAGSAGTQIVKD"
BASE COUNT          317 a          762 c          692 g          352 t
ORIGIN      
        1 ggtggcgagg acccggtcga acgccaccgg cgaaccgccc cgctgcacgt ggccgaggat
       61 cgtgacccgc gtctggaagc ccgttcgctt ctcgatctcg ggtgcgatgt gattggcgac
      121 cccgcccagc cgcgggaacc cgttctcgtc cacctcgggc tcgggaagga ccagcgtgcc
      181 ctcctgcggg atcgccccct cggccaccac cacgatcgag aacgagcttc gatgcatgtg
      241 ccggtggcgg agcatccggc acacgtcctc gatgtcgaac gggtgctccg ggatcaagat
      301 cacgtcggca ccgcccgcga tgcccgcgta gacggcgatc catccggcgt gacggcccat
      361 cacctcgacc agcatcacgc ggtcatggct ctcggcggtg ctgtgcaggc ggtcgatcgc
      421 atcggtggcc acctgcacgg ccgtgtggaa ccccacggtg atgtcggtgc ccgcgaggtc
      481 gttgtcgatc gtcttcggca ccccgaccac cggaaggcct cggtcggcga acgcggcggc
      541 cgcgcccaag gtgccttcac cgccgatggc gatcagaccg tcgaggcccc ggcgctcgaa
      601 ggtggcctcg accgcctccg gcccgccgtc gacgcggaag gggttcatgc cggaactgcc
      661 gaggatcgtg ccgccgcgat ggaggatgcc ggtgatctgc gggatgccga gcccgatcga
      721 atcgtcatcc acgacgcccc tccagccgtc acggaacccc atgaaggtgt ggccgtggac
      781 cccgacgccc ttgcggaccg cggcgcggat gacggcgttg agaccggggc agtcaccgcc
      841 ccccgtgatg atgcccagac gcatgcgcca agcctacgac gcccctggaa gccgccgagg
      901 gaggcgaacg tgtccgttac gcctcgtcga cagcctgggc cttgaggacc tggtaccagc
      961 tgtcgacgta ggacgcttcg tcgtcctccg tctggacatc ctgcctggcg tcttcgtcgg
     1021 tggccttcgc cgagcccccg aagccgaggt ggtcgtcggt ggatcgatcg atggcctgct
     1081 tgtcacccag cgacatatcc gctcctgcat cgtccttctc gtagacccat cggtcatcgg
     1141 gccgacaggc ggaacgcttg ataccgaacg tcgggtaacg cgtgcgaggc acggtccggc
     1201 cgtcgaacga gccgatagga ttggggaatg gtgatggcag ggctgttccc cgacgtcagc
     1261 aactggggct tcctcgaata cttcttcgtg gggctgctcc tgctgctggt ggccctggtc
     1321 accgtgttcg cgggatatgt gttcctgcag cagttccgca atcccgctcg gcgcggatcc
     1381 cggccctagt ccttcacgat ctgggtcccg gccgaaccgg ccagcgccgc ggtcgcatcc
     1441 gcgagatccg cgatcacgga ccgtccgccg ccgcgcgcga acgtcgccgc ggcctccacc
     1501 ttgggaccca tcgatccggc gccgaattcg cccttcctca ggagctgggt ggcttcccgc
     1561 accgtcatgc gatcgatgtc ctcgggcatg aacgagccgt agccccgctg caccttcttg
     1621 acgtccgtca ggatcaacag ggtggaagcc tcgacctcct tcgccaggat cgcagccgcc
     1681 cgatccttgt cgaccacccc ttccttgccg atcagccgcg ggccgtcctc gatcacgggc
     1741 acgcctcccc cgccgctcgc gatcacgatc gtgccggcgg ccaccagttg gcggatcacc
     1801 ggcgtctcca cgatcgagta cggcgacggg ctgggcacga cccggcgcca gcccccgtga
     1861 ggatcggcct tcatcacgta gccgcgttcg gattccaacg cccgcgcttc ctccggttcg
     1921 tagaagggac cgacgaactt cgtgggatcg gtgaacgcgg gatcgtcggg gcggacccgg
     1981 gtgagcgtga gcacggtggc caccggtcgc tccatgcccc gctcgaagaa cacgtcgccg
     2041 atcgtgacct gcaggaggta gccgatctgg ccctggctct cggcgccgca cacgtcgagg
     2101 ggcatcgggt gcacgacctc ccg
//
LOCUS       DATDBZ010000573         3634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_178249, whole genome shotgun sequence.
ACCESSION   DATDBZ010000573 DATDBZ010000000
VERSION     DATDBZ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178249"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VIX62_13190"
     CDS             <1..670
                     /locus_tag="VIX62_13190"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY5861230.1"
                     /translation="VSRPEVITKEIDGKRHEPLERGTCDVPDEHVGTVTQALAPRKGR
                     IVDLRQGDPGRTIVTFEAPSRGLIGFRSMLMTSTRGTALLHQHHAGWTPWAGELPHRM
                     GGAMVADRQGAVTAYALDNLQLRGELFVSPSDDVYEGMVIGENSRSDEMVVNAVRAKE
                     KNNIRTHSHDDAVKLPNPVVHTLETAIEWISDDELVEVTPNAIRIRKRLLGLEDRRRA
                     FKKN"
     gene            complement(674..1420)
                     /locus_tag="VIX62_13195"
     CDS             complement(674..1420)
                     /locus_tag="VIX62_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861231.1"
                     /translation="MPKRSEDAVPEDLHEWISFEDPSEDRTWVFDVTFLMSNWSCIFG
                     HGCRGVLTGPAEHLHQGCCSYGAHFIDDDDVAAVEVAVRRLTSTTWQLRSKGKKNWLT
                     TDDEGTRTTRVVNGACIFLNRPGFEGGIGCAFHIAALEHGERPLDWKPDVCWQLPLRL
                     HEHTDDHGHVTSTLREWKRRDWGEGGQEFHWWCTESSDAFVGGTPTYVYLRDEIVEFT
                     GPAVYQTLVAQLEARRTTTTMLPHPAIRSR"
     gene            1468..2892
                     /locus_tag="VIX62_13200"
     CDS             1468..2892
                     /locus_tag="VIX62_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5861232.1"
                     /translation="MTRDALVDDLRKALAADRVRDGAVELGLYRGDASNMTGGARVVC
                     FPTSTAEVQACVRLAQHHQVPFVARGSGTGLAGGAVPPTGAIVIVTTKMDKLLTVDVE
                     NRLAWVEPGMLNLDVSRQVAAHGLHFAPDPSSQQSCSIGGNVANNSGGPHCLADGVTT
                     SHVLALEVVLADGSVCVLGGEDPEPDGLDLRGAFVGSEGMFGIATRVCIRLTQNPPAV
                     RTMLLDFNRVEDGARTVSAIIAAGMVPAALEMMDALCTQAVEAYVHAGLPTDAAAILL
                     VEVAGLEHGLERQVDRISAIGREHGARTVRIAQDEAERALLWKGRKTAFGAIARIKPN
                     YYLHDTVVPRHKLTEVLNQVYEIAARHELLVMNVFHAGDGNLHPLLVFDKREPGVLDR
                     VHAAGEEIVRVSVAAGGVLSGEHGIGLEKRDLMPMMFGPDDLAAQAGLRAAFDPSGLA
                     NPLKVLPTAASCGDVQHVPEGAWI"
     gene            2883..>3634
                     /locus_tag="VIX62_13205"
     CDS             2883..>3634
                     /locus_tag="VIX62_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5861233.1"
                     /translation="MDLSRFADEVSSAGPVTIAGLCTRGGAVPGVRVVHAPVGIELVQ
                     PEEMTVSCGAGTPVHELADALAVHGQMVAMPPTGTVGGALAAGQSGIKRLGRGPIRDT
                     LLQTRYVSSTGRVVKAGGPTVKNVSGFDLCRLLVGSRGTLGFLGDVILRTWPLPAASR
                     WHGGPADPFDLLLRLYRPASILWDGTTTWVCLEGDAPDVDAQAVLCGLPEVAGPPELP
                     AGGRWSARPSALRQIADDAMGAGHRFVAEIGVG"
BASE COUNT          559 a         1221 c         1307 g          547 t
ORIGIN      
        1 ggtcagccgg cccgaggtga tcaccaagga gatcgacggc aagcgccacg aaccgctcga
       61 acggggcacc tgcgacgtgc ctgacgagca cgtcggcacc gtcacccagg cgctcgctcc
      121 ccgcaaagga cgcatcgtcg acctgcgcca gggcgacccg ggtcgcacga tcgtcacgtt
      181 cgaagcgcct tcccgcgggt tgatcgggtt ccgatcgatg ctcatgacct cgacgcgcgg
      241 caccgcgctg ctccaccagc accatgccgg atggacgccg tgggcgggtg agctgccgca
      301 ccgtatgggt ggggcgatgg tggccgaccg ccagggcgcg gtcacggcct atgcgctcga
      361 caacctccag ctccgcggcg agctcttcgt gagccccagt gatgacgtct acgaaggcat
      421 ggtcatcggc gagaactccc gcagcgacga gatggtggtc aacgcggtgc gggccaagga
      481 gaagaacaac atccgtacac acagccacga cgacgcggtg aagctgccga atccggtcgt
      541 gcacaccctc gagaccgcga tcgagtggat cagcgacgac gaactcgtcg aggtcacgcc
      601 gaacgcgatc cgtattcgca agcggctgct cgggctcgag gatcgtcgcc gggcgttcaa
      661 gaagaactga gcctcagcgg ctcctgatgg ccgggtgggg gagcatcgtc gtcgtggtac
      721 ggcgggcctc caactgcgca accagggtct ggtagaccgc cggccccgtg aactcgacga
      781 tctcgtcgcg caggtagacg taggtcggcg tgcccccgac gaacgcgtcc gacgactcgg
      841 tgcaccacca gtggaactcc tgaccaccct cgccccagtc gcgccgcttc cactcgcgca
      901 acgtggacgt gacatggccg tgatcgtcgg tgtgctcgtg cagccggagc ggaagctgcc
      961 agcagacgtc cggcttccag tcgagcggtc gttcgccgtg ctcgagcgcg gcgatgtgga
     1021 acgcacagcc gatgccgccc tcgaagccgg ggcggttcag gaagatgcag gcgccgttga
     1081 ccacacgtgt cgtgcgggtg ccctcgtcgt cggtggtgag ccagttcttc ttgcccttgc
     1141 tccgcagctg ccacgtggtc gaggtcaggc ggcgcacggc gacctcgacc gccgccacgt
     1201 catcgtcatc gatgaagtgc gcgccgtagc tgcagcagcc ctggtggagg tgctccgccg
     1261 gccccgtcag gacgccgcgg caaccgtgac cgaagatgca cgaccagttc gacatcagga
     1321 aggtcacgtc gaacacccat gtgcggtcct cactcggatc ctcgaacgag atccactcgt
     1381 gcagatcctc gggaacggcg tcctccgacc gctttggcat cggcgggacg atagtgcggt
     1441 cacagagcgg cgagtacggt ccgtcagatg acacgcgacg cattggtgga cgacctgcgc
     1501 aaagcgctcg ccgccgaccg cgtgcgcgac ggcgccgtcg agctcgggct ctatcgcggg
     1561 gacgcgtcga acatgaccgg gggagcgcgg gtcgtctgct tcccgacgtc gaccgccgag
     1621 gtgcaggcgt gcgtgcgact ggcgcagcac catcaagtgc cgttcgtggc ccggggatca
     1681 gggaccgggt tggcgggcgg cgcggtgcca ccgacgggtg cgatcgtgat cgtcacgacg
     1741 aagatggaca agctcctcac ggtcgatgtc gagaaccgtc tcgcgtgggt cgaacccggg
     1801 atgctgaacc tcgacgtgag ccgccaggtc gctgcccacg gtctccactt cgcccccgac
     1861 ccgtcgagtc agcagagctg ctcgatcggc gggaacgtcg ccaacaactc gggtgggccg
     1921 cactgcctcg ccgacggggt gacgacctcc catgtcctgg cgttggaggt cgtgctggcc
     1981 gacgggtcgg tgtgcgtcct cggtggcgag gacccggaac cggacgggct cgatctgcgg
     2041 ggcgcgttcg tcggcagcga aggcatgttc gggatcgcca cccgggtgtg catccgcctc
     2101 acgcagaacc ctcccgcagt gcggacgatg ctgctcgact tcaaccgggt cgaggacggc
     2161 gcccggaccg tcagcgccat catcgccgcc gggatggtgc ccgccgcgct ggagatgatg
     2221 gacgcgctct gcacgcaggc ggtggaggcg tacgtgcacg ccggtctgcc gacggacgcg
     2281 gcagcgatcc tgctcgtcga ggtggccggc ctcgaacacg gcctcgaacg ccaggtcgac
     2341 cggatcagcg cgatcggccg cgagcacggc gctcgaacgg tgcgcatcgc ccaggacgag
     2401 gccgagcggg ccctgctgtg gaagggccgc aagaccgcgt tcggcgccat cgcccggatc
     2461 aagccgaact actacctcca cgacaccgtc gtgccgcggc acaagctgac cgaggtgctc
     2521 aaccaggtgt acgagatcgc ggcgcgccac gaactgctgg tgatgaacgt cttccacgcc
     2581 ggcgacggga acctccaccc gctgctcgtg ttcgacaagc gcgaaccggg cgtgctcgat
     2641 cgtgtccacg ctgcgggcga ggagatcgtt cgggtcagcg ttgccgccgg tggcgtgctg
     2701 tcgggggagc acggcatcgg gctcgagaag cgtgacctca tgcccatgat gttcggaccc
     2761 gatgacctcg ccgcgcaggc cggtttgcgc gctgcgttcg acccgtccgg cctcgccaac
     2821 ccgctcaagg tgctccccac ggcggcgtcc tgcggcgacg tccaacacgt ccccgagggc
     2881 gcatggatct gagtcggttc gccgacgagg tgtcgtcggc cggacccgtg acgatcgcag
     2941 ggctgtgcac tcgcggtggc gccgtgccgg gcgtgcgcgt cgtgcacgcg ccggtgggga
     3001 tcgagctggt ccagccggag gagatgacgg tgtcgtgcgg ggcgggcacg ccggtgcacg
     3061 agctggccga cgcgctggcg gtgcacgggc agatggtggc gatgccgccg acgggcaccg
     3121 tgggcggggc cctggcggcc gggcagagcg ggatcaagcg gctcgggcgg ggcccgatcc
     3181 gcgacacgtt gctccagacc cggtacgtgt cgtcgaccgg gcgggtcgtc aaagccggcg
     3241 ggccgacggt gaagaacgtc agcggcttcg atctgtgccg tctcctcgtc ggctcgcgcg
     3301 ggacgctcgg cttcctcggt gacgtgatcc tgcgtacctg gccgctcccc gcggcgtcgc
     3361 gatggcacgg tggcccggcc gatccgttcg atctgctcct gcggctctat cggccggcgt
     3421 cgatcttgtg ggacggcacg acgacgtggg tgtgcctcga aggcgacgcg ccggacgtgg
     3481 acgcacaggc ggtgctgtgc gggctgcccg aggttgcagg cccaccggag ctgcccgcgg
     3541 gtggccggtg gtcggcccgt ccgagtgccc tccgtcagat cgccgacgac gccatgggtg
     3601 cgggccatcg gttcgtggcg gagatcggcg tcgg
//
LOCUS       DATDBZ010000574         7456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2843227, whole genome shotgun sequence.
ACCESSION   DATDBZ010000574 DATDBZ010000000
VERSION     DATDBZ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843227"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VIX62_13210"
     CDS             <1..623
                     /locus_tag="VIX62_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016443352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5861234.1"
                     /translation="EGFSVSVATDGRRAIDRFRSERPALVILDLMLPELSGLDVCRAI
                     RAESDVPIIMVTAKDSEADKVTGLELGADDYVTKPFSVRELVSRVRAHLRRAGMRADS
                     ALPEEEVLRGGPIEMDVSRHEVVVRGAPVGFPPKEFELLEAFLRRKDRLLTRLFLIEE
                     VWGADYVGDTKTLDVHVKRLRQKIETDPHEPTHVVTVRGLGYKFVD"
     gene            744..2816
                     /gene="ppk1"
                     /locus_tag="VIX62_13215"
     CDS             744..2816
                     /gene="ppk1"
                     /locus_tag="VIX62_13215"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY5861235.1"
                     /translation="MCAETFPEAMPATSARFLNRELSWLEFNARVLALAEDPARPLLE
                     RVKFVAIYTQNLDEFFQVRVSGLEEQVEAGVAVVTPDGMTPPEQLEAIRERLNGLQAR
                     VEGVMSAELLPALEKEGIRLARWDQLGEDDRAFLGRRFSERIFPVLTPLSVDPAHPFP
                     YISSLSLNVAAAVRDPMTGVRKFARVKVPQLLPRFIELPDGERFVPLEEVIAAHMDQL
                     FPGMDLLSHHFFRVTRDADVEVEEDEADDLLAAIETVLQRRQRGATPIRLEIDDTMSG
                     EVREVLMRELGLEESQVYVVDGLLALGDLWFFVSMDRPHLKDEPWTPVTPPELQSHEG
                     PPDVFEAMRRGDILVHHPYDSYESSVAAFVDQAARDPDVLAIKQTLYRTSADSAIIRS
                     LVRASNAGIQVVALVELKARFDEEANISFARTLETAGVHVVYGMVGLKTHAKTCLVVR
                     RENDGIRRYAHLGTGNYNSTTARLYEDIGLLTADPDLGADLTDLFNLLTGYSRQREYR
                     KLLVAPVTLRPGLAALVHREMEQDDGSIVLKMNSLVDSDLIDALYEASQSGVSVELLI
                     RGICCLRPGVPGLSEGIRVRSIVGRFLEHSRVYRFGSESRGFDYLIGSADWMPRNLDR
                     RVEALTPVEDGALQERLAEVLAIELADDELAWELDPDGGWHKVPTSRGANAHRALQEL
                     AVERAGER"
     gene            2813..4300
                     /locus_tag="VIX62_13220"
     CDS             2813..4300
                     /locus_tag="VIX62_13220"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEY5861236.1"
                     /translation="MNLEREVKLTVGPMFHLPDLSEVLDGFSAEGEGVQRFVTSYYDT
                     QDLRLARWGCSLRYRTGEGWTVKLPAAGLSDTLAREEHHFEAGPGRPPAPAIDLLRGY
                     LRQEPVSLAVRLQTVRRTTRLLDSEARPAAEVVDDEVSVLDGRRVVARFRELEAELTQ
                     DADEAIIDPVIKRLVAEGAQRAEPALPKYVRALMPLAAEPPEVEQHGKRPKTVEAMIR
                     TALSASVIRLIRHDAAVRLGSDLEGVHQARVATRRLRSDLRTFRTLLEPTWDAQLRDE
                     LRWLGDELGAVRDLDVLGERLRAHTSMLPDEDAVTMRKLLDRLRAQRDEARAAMLSAL
                     REDRYAVLLDRLVAAATAPAVLDDVASTPAVDVIDSLLSGPWDHLQRACESLDPGSAD
                     ADLHAARIRAKRVRYAAEAVAPVVGKPARRFAERAGVLQDELGEHQDSVVAAAWLRDQ
                     AAGTSARVAFTAGELGAIEARLQRRARRRWPDAWAALDRKKLRFW"
     gene            4300..5175
                     /locus_tag="VIX62_13225"
     CDS             4300..5175
                     /locus_tag="VIX62_13225"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5861237.1"
                     /translation="MKTEVKAAGGVVRRRGADGVRFALVHRPRYDDWTLPKGKAEDDE
                     DREDTARREVLEETGFRCELGPAIATVRYTDHLGRPKVVHYWLMYPAEGVFIPGDEVD
                     ELRWVTADEARELLTYGHDRVVLDAAIAFDRPIYLVRHAKAGDRDAWTEDDDLRPLTK
                     KGKQQAEGLVGLLEDRDVDRVLSSPSVRCVQTVRPIALARKVAVEEVPQLAEGTPARE
                     AIELLRTTGGGVVASSHGDVIPAVVTSLARRGVELVDAPEWKKGSTWVLERDGGLFTT
                     ARYLPPPGSAPGPSD"
     gene            complement(5172..5972)
                     /locus_tag="VIX62_13230"
     CDS             complement(5172..5972)
                     /locus_tag="VIX62_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5861238.1"
                     /translation="MTMLPDHVLRFWRALDERFGSVEAVWWGAVVTDARFPMIWDANY
                     ARVDVAAPDLRVADIEDHLLPALRDADATVMHVVMFHPDEATSLLSELSGRGDRLGWD
                     LVMDLEGEPPPPTEVAVVPLEGRVDLWDTVADSLVLFGAEPGEALEQFRALERDVLAP
                     AGKRWFGVVDQNGTVQSLGALVVLGDVGYVDNIATFPPFRGRGLASAVTSTIAREARR
                     AGANHVFLLADPEASAIVSMYERLGFGSAGSLASARGPIPGARSGSGG"
     gene            6013..7008
                     /locus_tag="VIX62_13235"
     CDS             6013..7008
                     /locus_tag="VIX62_13235"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEY5861239.1"
                     /translation="MVEGVVFRDGVRTDERAAIDGVPSLAKQPGVFVWIDVEDPTEAT
                     LGALAGEFRLHPLVVEDARHRRQRPKVELFSDYVFVVLRAAAVDDGDRSVMADLSETE
                     IHVFAGDGFLVTLRFSPCFDMEQTVRRWEGQPELLAQGAGFPLYVLFDEVVDGYLSAV
                     ERFEEMVDDLEDDIFAKEDGQGESPQVRERLFHLKRETVRLRRFVSPLREGIEFVIGR
                     QELAGDELAPYYRDVVDHVIRADELLDNVRDLLTSLQELRVAQAANRVNEVMKSLTAW
                     ATILLVPTLFAGIWGMNFRHMPELQWRFGYAFAVGTILASALGLYAWFKWKKRWL"
     gene            6987..7343
                     /locus_tag="VIX62_13240"
     CDS             6987..7343
                     /locus_tag="VIX62_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861240.1"
                     /translation="MEEAVAVMCRALKVLCVAPDDGSLMALKSATVSAEWELTPGATD
                     LRDALDQIDVERPHVMVAFGPFDDLIAIARERFQGMRIVTDHDAPGATVVATSLDEVR
                     GVVLGLPHPGGPVSGG"
BASE COUNT         1174 a         2438 c         2672 g         1172 t
ORIGIN      
        1 gggaagggtt ctcggtgtcg gtcgcgaccg atgggcgacg tgcgatcgac cggttccgat
       61 ccgagcgtcc cgcgctcgtg atcttggatc tgatgcttcc agagctctcc ggcctcgacg
      121 tgtgccgcgc catccgggcg gagtccgacg ttcccatcat catggtgacg gcgaaggact
      181 cggaggccga caaggtcacc ggtctggagt tgggagccga cgactacgtc acgaagccgt
      241 tctcggtgcg ggaattggtc tctcgggtcc gagcgcacct gcgtcgtgca gggatgcgag
      301 ccgacagcgc cctgcccgaa gaagaggtgc ttcgcggtgg tcccatcgag atggatgtgt
      361 cgcggcacga ggtcgtggtg cggggcgcgc cggtggggtt cccacccaag gagttcgaac
      421 tgctcgaggc gttcctccga cgcaaggatc ggttgctcac acggctgttc ctgatcgagg
      481 aggtctgggg ggcggactac gtgggtgaca ccaagaccct cgacgtgcac gtgaagcggc
      541 tccgccagaa gatcgaaacc gatccccacg aaccgaccca cgtcgtcacc gtgcgcgggc
      601 tcgggtacaa gttcgtcgac tgacgcccac cgggcatcga tcacggtctg ccgcgacaca
      661 ggttgcaccg caaggtatcc gtgatgtgga cggagtgtgc gattgcttgg gaggctggga
      721 ctctctagga tgtcgacccc accatgtgcg ccgagacctt ccccgaggcg atgcccgcca
      781 cttcagcccg cttcttgaac cgcgagctga gttggctgga gttcaatgcg cgggtgctgg
      841 ccctcgccga ggatcctgcg cgcccgctgc tcgaacgggt caagttcgtg gcgatctaca
      901 cgcagaacct cgatgagttc ttccaggtcc gggtttccgg tctcgaggag caggtggagg
      961 ctggggtcgc ggtggtcacg cccgacggga tgacgccgcc ggaacaactc gaagcgatcc
     1021 gcgagcggct gaacggactg caggcgaggg tcgaaggcgt gatgtccgcc gagctgctcc
     1081 ccgccctgga gaaagagggc atccggctcg cccgctggga tcaactcggc gaggacgacc
     1141 gggcgttcct tggccgtcgg ttctccgaac ggatcttccc cgtgctcacg ccgctgtcgg
     1201 tggatccggc gcatccgttc ccctacatct cgagcctgtc gttgaacgtc gcggcggccg
     1261 tgcgcgaccc gatgacgggg gtgcgcaagt tcgcgcgcgt gaaggtgcca cagttgctgc
     1321 cccggttcat cgagctgccc gacggcgagc gcttcgtgcc gttggaagag gtgatcgcgg
     1381 cgcacatgga ccagctgttt cccggcatgg acctgttgtc ccaccatttc ttccgcgtga
     1441 cacgcgacgc cgacgtcgag gtcgaggagg acgaagccga cgacctgctc gctgcgatcg
     1501 agaccgtcct gcaacgtcgg caacgtggtg ccacgccgat ccgcctggag atcgacgaca
     1561 cgatgagcgg cgaggtgcgc gaggtcctga tgcgcgagct gggcctggag gaatcccagg
     1621 tctacgtggt cgacggactc ctcgcgctcg gcgatctctg gttcttcgtg agcatggatc
     1681 gaccccatct caaggacgaa ccctggacgc cggtcacgcc acccgagctc cagtcgcacg
     1741 agggcccacc ggacgtcttc gaggcgatgc ggcgcggcga catcctcgtg catcacccct
     1801 acgactccta cgaatcctcc gtcgcggcct tcgtggatca ggcggcgcgg gacccagacg
     1861 tcctcgcgat caagcagacc ctctatcgca catccgccga cagcgcgatc atccgctccc
     1921 tggtgcgagc ctccaacgcc ggtatacagg tggtggctct cgtcgagttg aaggcgcgat
     1981 tcgacgagga agccaacatc tcgttcgcaa gaacgctgga aacggctggg gtgcacgtgg
     2041 tgtacgggat ggtgggcctc aagacgcacg cgaagacctg cctggtggtg cgccgggaga
     2101 acgacggcat ccgacggtac gcgcatctgg ggacgggcaa ctacaactcc acgaccgcgc
     2161 gactgtacga agacatcggg ctgttgacgg ccgaccccga tctgggcgcg gatctcacgg
     2221 acctgttcaa cctgctcacc ggctacagcc ggcagcgcga gtaccgcaag ctgctggtgg
     2281 ccccggtcac cctgcgcccc ggcctcgcgg ccctggtcca ccgggagatg gagcaggacg
     2341 acggctccat cgtgctgaag atgaacagcc tcgtggacag cgacctgatc gatgccctct
     2401 acgaagcgtc acagtcgggc gtcagcgtcg agttgctgat ccgaggcatc tgttgtctgc
     2461 gtcccggggt cccggggctg tccgagggca tcagggttcg ctccatcgtt ggccggttcc
     2521 tggagcattc ccgcgtgtac cggttcggga gcgagtcgcg cgggttcgac tacctgatcg
     2581 gatcggccga ctggatgccg cgcaacctcg accgacgcgt cgaggccctc acgcccgtgg
     2641 aggacggcgc tctgcaggag cgcctggccg aggtgctggc gatcgaactc gccgacgacg
     2701 agcttgcatg ggagctcgat cccgatggag gatggcacaa ggtgccgacg agccgcggcg
     2761 cgaacgccca ccgggcgctg caggagctcg ccgtggagcg ggctggcgag cggtgaacct
     2821 ggagcgcgag gtcaagctca ccgtcggtcc gatgttccat cttcccgacc tgtccgaggt
     2881 cctcgacggc ttctccgccg aaggcgaggg cgtccagcgg ttcgtcacca gctactacga
     2941 cacgcaggac ctacggctgg ctcgctgggg atgctccctg cgctaccgga ccggtgaggg
     3001 gtggaccgtt aagctccccg ctgcgggcct gtccgacacg ctggcgcgcg aggagcatca
     3061 cttcgaggcg ggcccgggcc gtccgccggc tcccgcgatc gacctgctcc ggggatacct
     3121 gcggcaagag ccggtgtcgc tcgccgtgcg gctgcagacg gtccgccgga ccacgcggct
     3181 gcttgattcc gaggccaggc cggccgccga agtggtggac gacgaggtct cggtcctgga
     3241 cgggcgacgg gtggtggcgc gcttccggga gctcgaagcc gaactcaccc aggatgccga
     3301 cgaagcgatc atcgacccgg tgatcaaacg gctggtggcc gaaggggcac aacgcgccga
     3361 accggccttg ccgaagtacg tccgggccct catgccgttg gcggccgaac cgccggaggt
     3421 ggagcagcac gggaagcggc ccaagaccgt cgaggcgatg atccgcaccg ccctgtcggc
     3481 ctcggtgatc cggttgatcc gacacgacgc cgccgtccgg ctcggcagcg acctggaagg
     3541 cgtgcaccag gcgcgggtgg cgacccggcg gctccgctcg gacctccgga cgttccgaac
     3601 gttgctggag ccgacgtggg acgcccagct tcgggacgaa ctgcgctggc tcggcgacga
     3661 gctcggagcc gtccgtgatc tcgacgtgtt gggcgaacgg ttgcgggcgc acacctcgat
     3721 gctccccgac gaggacgcgg tcacgatgcg caagctcctc gatcggttgc gcgctcaacg
     3781 cgacgaagca cgcgcggcca tgctgtcggc cctgcgagag gaccggtacg ccgtgctgct
     3841 cgatcgtctc gtcgcggccg ccacggcgcc cgcggtcctc gacgatgtgg cgtccacccc
     3901 cgccgtcgac gtgatcgact cgctcctgtc agggccgtgg gaccacttgc agcgcgcgtg
     3961 cgagtcgctg gatccgggat cggccgatgc cgacctgcat gcagcacgga tccgcgccaa
     4021 acgcgtccgg tatgccgcag aggcggtggc ccctgtggtg ggcaagccgg cccgcaggtt
     4081 cgccgagcgg gccggtgtgc tgcaggacga actgggggaa caccaggatt cggtggtggc
     4141 cgcagcgtgg ctgcgtgatc aggccgccgg gaccagcgca cgggtggcgt tcacggcagg
     4201 tgagctcggc gcgatcgagg cccgcctcca gcgacgagcg cgccgccgct ggcccgacgc
     4261 gtgggccgcg ctcgaccgca agaagctgag gttctggtga tgaagaccga ggtcaaggcc
     4321 gccggtggtg tcgttcgccg gcgtggcgcc gacggggtgc ggttcgcgct cgtccaccgg
     4381 ccacgctacg acgactggac cttgcccaag ggcaaggccg aggatgacga ggatcgagag
     4441 gacaccgcgc gccgggaggt gttggaggag accggcttcc ggtgcgagct cggaccggcg
     4501 atcgccaccg tgcgctacac cgatcacctt ggccgtccca aggtggtcca ctactggctg
     4561 atgtatccgg ccgaaggcgt gttcatcccg ggcgacgagg tggacgagct ccggtgggtg
     4621 accgccgacg aggcccgtga actcctgacc tacggccatg accgggtggt gctggacgcc
     4681 gcgatcgcgt tcgaccggcc gatctacctg gtgcgacacg ccaaggcggg ggaccgcgat
     4741 gcctggacgg aggacgatga cctccggccg ctgacgaaga agggcaagca gcaagccgaa
     4801 gggctggtgg ggctgttgga ggatcgcgac gtcgatcgcg tgctctccag tccgtcggtg
     4861 cgctgcgtgc agaccgtccg tccgatcgcg ttggcgcgca aggtcgcggt cgaggaggtc
     4921 ccgcagctgg ccgaaggcac gccggcgagg gaagcgatcg agctgctccg caccacgggc
     4981 ggcggggtgg tcgcctcgag ccacggtgac gtgatcccgg cggtggtgac atcgctggca
     5041 cgccgtggcg tcgagctcgt cgacgcaccc gagtggaaga agggctcgac ctgggtgctc
     5101 gagcgcgacg gcgggctgtt caccacggcg cggtatctgc cgcctccggg gtcggcgccg
     5161 ggtccctcgg actagccgcc ggagcccgaa cgtgcgccgg ggatcggtcc cctggccgaa
     5221 gccaggctgc ccgccgaacc gaacccgagg cgttcgtaca tcgagacgat cgctgaggcc
     5281 tcaggatcgg ccaacaggaa cacgtggttc gcgcccgcgc gccgcgcttc ccgtgcgatc
     5341 gtggaagtga cggcgctcgc gagcccgcga cctcggaacg gcgggaacgt ggcgatgttg
     5401 tccacgtagc ccacgtcgcc gagcaccacc aacgcaccga ggctctgcac cgtgccgttc
     5461 tggtccacca cgccgaacca ccgcttgccg gcgggcgcga gcacatcgcg ttcgagcgcg
     5521 cggaactgtt cgagcgcctc ccccggctcg gcgccgaaga gcaccaggga gtcggcgacc
     5581 gtgtcccaca ggtcgacgcg accctcgagt gggaccaccg cgacctcggt tgggggcggt
     5641 ggctcgccct cgaggtccat gaccaggtcc cacccgagcc ggtcgccgcg gccggacagt
     5701 tccgacaaca gcgacgtggc ctcgtccgga tggaacatca ccacgtgcat caccgtggcg
     5761 tccgcatcgc gcagggccgg cagcaggtgg tcctcgatgt cggcgacgcg aaggtccggg
     5821 gccgcgacgt cgacccgggc gtagttcgcg tcccagatca tggggaagcg ggcatcggtg
     5881 accacggctc cccaccacac cgcttccacc gacccgaacc gctcgtcgag cgcgcgccag
     5941 aaccggagca cgtggtcggg gagcatggtc atcgcccgga gtgtagcgac gaccatccgt
     6001 taggctccga ccatggtgga aggggtcgtg ttccgggacg gcgtccgcac ggatgaacgc
     6061 gccgcgatcg acggggtccc ctcgctggcg aagcagcccg gggtgttcgt ctggatcgat
     6121 gtcgaggacc cgacggaggc caccctcggg gcgctcgccg gggagttccg gctgcatccc
     6181 ctggtggtgg aggacgctcg ccatcgccgt caacggccga aggtggagct gttcagcgac
     6241 tacgtgttcg tggtgttgcg agcggccgcc gtcgacgacg gcgatcgcag cgtgatggcc
     6301 gacctctccg aaaccgagat ccacgtgttc gccggtgacg ggttcctggt gaccctccga
     6361 ttctcgccct gtttcgatat ggagcagacg gtgcgtcgat gggaaggcca accggagctg
     6421 ctggcgcagg gcgccgggtt ccccctctac gtgctgttcg acgaggtggt ggacggctac
     6481 ctcagcgcgg tcgagcggtt cgaggagatg gtggacgatc tcgaggatga catcttcgcg
     6541 aaagaggacg ggcaagggga gagcccgcag gtccgtgagc ggctgttcca tctcaagcgc
     6601 gagacggtgc ggctgcgtcg cttcgtctcg ccgctgcgcg aggggatcga gttcgtgatc
     6661 ggacgccagg agctcgcggg cgacgagctg gcgccgtact accgcgacgt ggtggatcac
     6721 gtgatccggg ccgacgaact gctcgacaac gtccgggacc tgctgacgtc gctgcaggaa
     6781 ctgcgggtgg cgcaggcggc caaccgggtc aacgaggtga tgaagagcct gacggcgtgg
     6841 gcaacgatcc tgctggtgcc caccttgttc gccggcatct ggggcatgaa cttccgccac
     6901 atgcctgagc tccagtggcg gttcggctac gccttcgcgg tgggcacgat cctggcttcg
     6961 gcgctggggc tgtacgcctg gttcaagtgg aagaagcggt ggctgtgatg tgccgggcgt
     7021 tgaaggtgct gtgcgtcgcg cccgacgatg gatcgctcat ggcgttgaag agcgccacgg
     7081 tgtcggccga gtgggagctc acgccgggcg cgaccgacct ccgcgatgcg ttggaccaga
     7141 tcgatgtcga acggccccac gtgatggtgg cgttcggccc gttcgatgac ctgatcgcga
     7201 tcgcccggga gcgcttccag gggatgagga tcgtgacgga ccatgacgcg cccggggcca
     7261 ccgtggtggc cacctcgctg gacgaggtgc ggggcgtggt gctggggctg ccccacccgg
     7321 gcggtccggt cagtgggggt tagccccccc aggggcccca accacctcgg tgcaccatgc
     7381 gcatggtcac catgatgttg gcgcgcgcat tgaacgccga ccagccccgg aatccgtaca
     7441 cacgggcccg gccagg
//
LOCUS       DATDBZ010000575         3377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_594378, whole genome shotgun sequence.
ACCESSION   DATDBZ010000575 DATDBZ010000000
VERSION     DATDBZ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594378"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VIX62_13245"
     CDS             complement(<1..833)
                     /locus_tag="VIX62_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5861241.1"
                     /translation="MLDVLWLIPAFPLAGFLIILLLGRRLGEPLAGWVATLMVAASFV
                     VSVGVYIDMLGLSAEERSHTDVIFTWIPVGALHVDLALLADPLSIIMCLFVTGVGALI
                     HMYSIGYMHGDPKFTKYFLYLNLFVFAMLLLVLGENMLVTFLGWEGVGTCSYFLIAFW
                     HTRESAATAGKKAFVTNRIGDWGFMIAMFLAFSTVGSLSFGVLNNNAESGLLSPTTAT
                     AIAALLFVGAVGKSAQLPLFIWLPDAMAGPTPVSALIHAATMVTAGVYLMVRVNPVLT
                     VA"
     gene            complement(840..1139)
                     /gene="nuoK"
                     /locus_tag="VIX62_13250"
     CDS             complement(840..1139)
                     /gene="nuoK"
                     /locus_tag="VIX62_13250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY5861242.1"
                     /translation="MEPTWYLLLSALLFSIGAVGLLIRRNPLVMVMCIELMLNAANLS
                     FVALSVQLGDIDGQTAVFFVMVVAAAEAVVGLGILVSILRARPGATADDLAELKG"
     gene            complement(1148..1735)
                     /locus_tag="VIX62_13255"
     CDS             complement(1148..1735)
                     /locus_tag="VIX62_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY5861243.1"
                     /translation="MNATFIEYVVFFSAAVMILGGAVGVIAMKSPVHAALGLVLTLFG
                     VAVQFVALGAHFVAVVQIIVYAGAIVVLFLFVIMLLGIDRSMDLQVEPIPLQRPLALV
                     MGVGLVGLLGAAIVRARDAVAINPGTGLAVAGENAGHDANIQQLGASLYGDYVFALEL
                     ASVLLIVAVTGTVLVTRRWPRSASLGDGGQGGSDQ"
     gene            complement(1839..2471)
                     /gene="nuoI"
                     /locus_tag="VIX62_13260"
     CDS             complement(1839..2471)
                     /gene="nuoI"
                     /locus_tag="VIX62_13260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY5861244.1"
                     /translation="MGWLSGFGVTLRQIGRPKVTEHYPKEKRPKPVRFHGRHVLNRYE
                     DGMEKCIGCELCAGVCPAKCIYVRGADNPIDAPVSPGERYGFVYEINYLRCIHCDLCV
                     EACPTEAITESKRFEFSFTNRKDAIYTKAELVVGDDGKPKQLPWEDWREGDDVLTSGW
                     MRATSPSGDATFEGQVGWSGELGYGVRAPEPEQTERDRSVESKGTRVVGR"
     gene            complement(2471..>3377)
                     /locus_tag="VIX62_13265"
     CDS             complement(2471..>3377)
                     /locus_tag="VIX62_13265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY5861245.1"
                     /translation="PAFLAFSIVPIGGVVTIAGHETFLQLADLPFGILWLLAMSGLGL
                     YGVLLAGWASGSKYPLLGSVRASAQLLSYEAAFGLAIVGVLVQSNTLSTRGIVIKQGW
                     DGVESIFNGDWYWLPAIVALVIFVISAVAETNHPPFDLVEAEQELTGGFFTEYTGIKF
                     AIFYLAEFMNVITMCAIAVTLFFGGPNGPGLGFLPANGWFNAWIMPVFWFMFKVIILL
                     FGTVWLRASLPRLRYDQLMGLGWKFLIEIAFIWVMISGVVIVAKEEGWSMWIVLPAAI
                     AGAVAIGTVLYLSVPKKTELVEEIR"
BASE COUNT          653 a         1126 c         1133 g          465 t
ORIGIN      
        1 gccaccgtga gcacggggtt gacgcgcacc atcaaataca cgccggcggt gaccatcgtg
       61 gcggcgtgga tcaacgccga caccggcgtc gggccggcca tcgcgtccgg gagccagatg
      121 aacagcggga gctgcgccga cttgccgacc gcaccgacga acaacagcgc ggcgatggcc
      181 gtcgctgtgg tcggcgacag caggcccgac tcggcgttgt tgttgagcac cccgaagctg
      241 agtgagccga cggtcgagaa ggcgaggaac atcgcgatca tgaatcccca gtcaccgatg
      301 cggttggtga cgaaggcctt cttgccggcg gtggcggccg attcacgggt gtgccagaag
      361 gcgatgagga agtacgagca ggtaccgacg ccctcccaac cgaggaaggt gaccagcatg
      421 ttctcgccga gcaccagcag cagcatggcg aacacgaaca ggttgaggta caggaagtac
      481 ttcgtgaact tcgggtcgcc gtgcatgtac ccgatcgaat acatgtggat cagggcgccg
      541 acgccggtga cgaacaggca catgatgatc gacaacgggt cggccagcag cgcgaggtcg
      601 acgtgcagcg cgcccaccgg gatccacgtg aagatcacgt cggtgtgcga acgctcctcg
      661 gcgctcaacc cgagcatgtc gatgtagacg ccgaccgaca ccacgaacga ggcggccacc
      721 atcagcgtgg cgacccaacc ggcgagcggt tcgcccaggc ggcggccgag gagcaggatg
      781 atcaggaatc cggccagtgg aaaggcaggg atcaaccaca gcacgtcgag cacggcgcgt
      841 cagcccttca actcggcgag gtcgtcggcg gtggcaccgg ggcgagcacg caggatcgac
      901 acgaggatgc cgaggcccac cacggcctcg gcggcggcaa ccaccatcac gaagaacacc
      961 gccgtctggc catcgatgtc gccaagctgc accgacaggg cgacgaaact caggttcgcc
     1021 gcgttgagca tcaactcgat gcacatgacc atcacgagcg ggttgcgccg tatcaggagc
     1081 ccgaccgcgc cgatcgagaa cagcagcgcc gacagcagca ggtaccaggt cggttcgatg
     1141 acggcgatca ctggtccgac ccgccttgcc cgccgtcgcc gaggctggcc gaccgtggcc
     1201 agcgccgcgt cacgagcacc gtgcccgtca cggccacgat cagcagcacc gaggccagtt
     1261 cgagggcgaa cacatagtcg ccgtacaggc tcgcgccgag ctgttggatg ttggcatcgt
     1321 gaccggcgtt ctcgccggcg acggcgaggc cggtgccggg gttgatggcc accgcgtcac
     1381 gcgcccgcac gatcgcggcg ccgagcaggc cgacgagccc gacgcccatc acgagcgcca
     1441 gtggacgctg gagcgggatc ggttcgacct ggaggtccat gctgcggtcg atgccgagca
     1501 gcatgatgac gaacaggaac agcacgacga tcgcgccggc gtacacgatg atctgcacca
     1561 cggcgacgaa gtgggcgccg agcgccacga actgcacggc cacgccgaac agggtgagca
     1621 cgaggccgag cgccgcgtgg acggggctct tcatcgcgat gacgccgacc gccccgccga
     1681 ggatcatgac tgccgcggag aagaacacca cgtattcgat gaacgtggca ttcattcggg
     1741 cgccaccaat cggtgaacgg gttccgtccg cgaccgaggt ggcgcctcga atgagcgcga
     1801 gcgctcgccg tcctgatggg tgagctcgct tcgctcgctc accggccgac caccctggtg
     1861 cccttcgact ccaccgaccg gtcgcgctcg gtctgctccg gctctggtgc gcgcacgcca
     1921 tagccgagtt cgccgctcca gcccacctgg ccctcgaacg tggcgtcgcc cgacggcgac
     1981 gtcgctcgca tccagccgct ggtcaggacg tcgtcgccct cacgccagtc ctcccacggc
     2041 agctgcttcg gcttgccgtc gtcgccgacg acgagttcgg ccttggtgta gatggcgtcc
     2101 ttgcggttgg tgaaggagaa ctcgaaccgc ttcgactcgg tgatggcctc ggtcgggcag
     2161 gcctcgacgc acaggtcgca gtggatgcag cgcaggtagt tgatctcgta gacgaagccg
     2221 tagcgctcgc ccggcgagac cggcgcgtcg atcgggttgt cggcgccgcg cacgtagatg
     2281 cacttcgccg ggcacacgcc cgcgcacagc tcgcagccga tgcacttctc catcccgtcc
     2341 tcgtaccggt tcagcacgtg acggccgtgg aagcgcaccg gcttcgggcg cttctccttc
     2401 ggatagtgct cggtgacctt gggccggccg atctggcgga gcgtgacccc gaacccgctg
     2461 agccagccca tcagcggatc tcctcgacga gctcggtctt cttcgggacc gagaggtaga
     2521 ggacggtgcc gatggcgacc gcgccggcga tggcggcggg caggacgatc cacatcgacc
     2581 agccctcctc cttcgccacg atcacaacgc ccgagatcat cacccagatg aaggcgatct
     2641 cgatgaggaa cttccagccg agacccatca gctggtcgta ccgcaggcgc ggcagcgacg
     2701 cgcgcagcca cacggtgccg aacagcagga tgatcacctt gaacatgaac cagaacaccg
     2761 gcatgatcca cgcgttgaac cagccgttgg cggggaggaa gccgagcccc ggaccgttgg
     2821 gcccgccgaa gaacaaggtg accgcgatcg cgcacatcgt gatcacgttc atgaactcgg
     2881 cgaggtagaa gatcgcgaac ttgatgccgg tgtactcggt gaagaagcca ccggtgagct
     2941 cctgctcggc ctcgacgagg tcgaacggag ggtggttggt ctcggcgacg gccgagatca
     3001 cgaagatcac cagcgcgacg atcgcgggca gccagtacca gtcaccgttg aagatcgact
     3061 cgacgccgtc ccacccctgc ttgatcacga tgccgcgcgt cgagagcgtg ttcgactgca
     3121 cgagcacgcc gacgatcgcg aggccgaacg cggcctcgta gctgagcagc tgcgccgacg
     3181 cgcgcaccga gccgagcagc gggtacttgg accccgacgc ccacccggcg agcagcacgc
     3241 cgtagagccc gaggcccgac atcgccagca accagaggat gccgaacggg aggtcggcga
     3301 gctggaggaa cgtctcgtgg ccggcgatgg tgacgacgcc accgatgggc acgatgctga
     3361 acgcgaggaa cgcgggc
//
LOCUS       DATDBZ010000576         9811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_594549, whole genome shotgun sequence.
ACCESSION   DATDBZ010000576 DATDBZ010000000
VERSION     DATDBZ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594549"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VIX62_13270"
     CDS             <1..790
                     /locus_tag="VIX62_13270"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY5861246.1"
                     /translation="GATSSGVNSSHTYAAAGSYPIALTVTDDDGATGSTISTVTVTSP
                     GGPSVLASDAFQREVINGLGTADVGGPWTTSGGSTAFSVTGGTGRIRMATPGGTLNAY
                     LDGAPQQAGDLRLSFASDKPATGSGVYVSVIGRRVAAIGAYQAKIVLRAGGTVGISLV
                     RVNASGGAEVALQAAINVPGLAYVAGDRLNVRLQVVGASPTTLNLKVWKAGTTEPSAW
                     QRTATDSTAGLQTAGGVGLRAYLSSTSTNAPVIASFDDLVLTVP"
     gene            1061..1933
                     /locus_tag="VIX62_13275"
     CDS             1061..1933
                     /locus_tag="VIX62_13275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5861247.1"
                     /translation="MFSRSSFLQAPMNGLLIPRIAKRWGADVSVVHNFSALRGYSATF
                     IHDFMFMDHARWFTCIENAYFRLMVATSRRASLLLTSSQTEANRVFRISGREATPIGL
                     GLRSALVESEPTRPRAVATEGQAFVMAVGRLNARKNLAMAIEGYLASGLNVDTRLVVV
                     GERSGRSELLSRPALDAIEGGLVRLIGSVSDGELRWLYEHARAFLMLSLDEGFGLPLA
                     EARYFGTPAVVSDLPVFHEVLGDYGTFVNPTDPTDIARGILGVRRGSTKGERHQWEDS
                     VRIMRSKIAERLRS"
     gene            1930..2853
                     /locus_tag="VIX62_13280"
     CDS             1930..2853
                     /locus_tag="VIX62_13280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5861248.1"
                     /translation="MKRNEDGAEADRLALVVVNFASSSLLAANLVDPGLRGAIGCIVV
                     VDNPSTADETRRVRELCVTNDWALVELARNEGFGAGVNAGVARARQLGCTRVLALNPD
                     ATIDADSARELLAASRADPRALIGPHIVRPDATVWFAGAVLDPRAGTTRRAREEELDG
                     ETTWQTGACFLATITMWDEVGGFDDDYFMYWEDIDLSWRWREAGGRLIVHENATAVHD
                     AGGTQAGSGKSPLYVYFNCRNRLLFARKRFRSRYGRRWWRGSISYGWSVATRGSRRML
                     AKHPLLLWSALRGTLAGAFGSLTPRYASARR"
     gene            complement(2822..6175)
                     /locus_tag="VIX62_13285"
     CDS             complement(2822..6175)
                     /locus_tag="VIX62_13285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5861249.1"
                     /translation="MRVMQTFGEPRGTTNPYIVMLRAALIEEPAVEHIPFSWRAALTG
                     RYDVMHIHWPDALLAARHWWTRAGKRVALACLVTRVRLRRVAVVRTVHNITPPSGPFL
                     DRALIRALERHTDVRIRIAAVTPEVAGVPSVLIPHGHYRTWFDGMPRAATVPGRLGYA
                     GLIKPYKGVERLVDAFAEASTIDPTLTLRISGKPANEAIERRLLTATERLSGLDTTLR
                     YVSEQAFVDVVTSSELVVLPYRFMHNSGTALAALSLGRPILVPGNDLNRALSAEVGAG
                     WVHLFDGELTAGMLLDAIQLVRAEPPRAAPDLSARGWHDVGARHARAYQLAIDRRRGR
                     SPVASAPTPTRGHVSELTVAIPTFRRSEALANALSAVREQVDAENALPAAPMRCSILV
                     IDNDPAGSGERIAAEHGARYVVEPRPGIAAVRNRALEECANGGVLLFIDDDETPEPGW
                     LRAMIAMYMTTGATAVAGRVVTRLPDTAERWGSRNAFERPTRTHGQLMSEAATNNLLL
                     DLDQIRRLGLSFDERFGLTGGSDSLFTLQLTRGGGTIRWAENAVVVEQDDPARHTRSW
                     ILMRVFRFGNTSARVRIALAPTAALRLVERVLALGRGATRAVGGSMRWAFGMVSRSPH
                     HRAHGLRIASRGFGMIAGAVGYAHDEYGRRRSSTTPSDPQSGHRTYAGGMRLKQWSFT
                     VKSRTVRRSSGEYLAELLRNQHLSADELARLQSHRAASIARFAAESTGYYRRLFADHG
                     IDAARLDDPAEWERIPITERAQLKEHDAELRPANSERFAREALTGGSTGVPLRTAQDS
                     RVPSLALAWRMYSWWGVQPWDDLARLGRWGFGRFDTLRNRLQWWPSKQLYLDASLMDA
                     DSMREFHRAIVRTRPALIEGYVGGVLDFADFLEAEGLTIPTPIAIATTASPLPPSTRR
                     RIESVLGAPVYDEYRGAEMSWAAGECRMQDGLHIFADAKKIEVVDTNGRPLPAGEVGD
                     IVVTDLANRVFPIIRYRLGDRGSLIDGTCACGVSLPRMAQPDGRITDVLRLPSGMAVT
                     RLTVMFAKHPESVRLFQIRQNADYSITVRVVLGTGTNATRHIEDEVEILRRRVAGEVP
                     VTLEYVDSLPHTGAKTKYVISDVPTRTAE"
     gene            6381..7979
                     /locus_tag="VIX62_13290"
     CDS             6381..7979
                     /locus_tag="VIX62_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861250.1"
                     /translation="MKHKKQKHKKQQTTKKKFALVALLTASIALAPIAALTAVAPASA
                     TTVSSPSKTLQPGSSYVTRAAGSWTDGTATLSTKFAAVPTRGGGLYTGVRVRESNGSY
                     YLAHTRTYPDGTVQASIRRIVQTNGTSAETLLSTRESVATKAKAGSAFSIRLQAEGTD
                     TVKLRATITVGSGTPLVLTATDSSSSAVDRAGTAAGWGYLSTSTARIAIAPATVESTP
                     TAATATPTPTPTPTPTPTPTPTPTPAPTPTPTPTPAPTPAPTPGPAPSNPSPAPGAYP
                     SAETTGVPAGTKLTVHQGDLTVTTAGAVIDGVEVHGVLTVKAPNVVIKNSRILGRAVD
                     NHIGLVNNIVSGAPFTIQDSEIYAAKPSAWQNGIFGSNFTATRVEIHGVIDPIKVIGD
                     NVEVRDSWLHDNLHYEQDESRGGTPSHDDSIQIEAGKNISITGNRMEGADNAAIQITQ
                     NTSKTPLGNVRISDNSFNDGGCTVNIAKTPTSIGDFVVTDNVFGPDRKFAKCGILAPA
                     MNTPQQSGNTWEATGTAVVQTVLK"
     gene            complement(8002..8868)
                     /locus_tag="VIX62_13295"
     CDS             complement(8002..8868)
                     /locus_tag="VIX62_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861251.1"
                     /translation="MNRSEGLVVAVTGRYHGLGNRMRVVLGAQALARYEGRSFAYVWP
                     TGREFGARLDELWEFRGRRWTTIRSRMVAKAYPYHGAGLTWIDDAARQVRVWQIRTPH
                     ALTLPPGAPSWHAALRALQPVEGIRRTILELAAASADRPYLGVMIRTHSVAHDATLRE
                     SPVEWYLSRIQEIRSERPELGLYVAADTDAAFVQVARRFPDCVGQTDKGAYNSRPALQ
                     AAVADLYLLAGSCHILGPHYSSFPELARFLSGNAVGLETSTSAPETRFEVQGEPTAGT
                     PLRPELRSPGRI"
     gene            complement(8855..>9811)
                     /locus_tag="VIX62_13300"
     CDS             complement(8855..>9811)
                     /locus_tag="VIX62_13300"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEY5861252.1"
                     /translation="TPAIFQSSPNKQQDAMLGAIIGFLLSSFAVVLASALDTRVRTEA
                     TLKSITELPVLGSIEQTPSSSDPRPVAIRQPNGSAAESFRHVRSGLRFASASHETRSL
                     AITSSRPAEGKTWTAGNLALVLAETGKRVLLVDADLRKPKVADAFGIVGAVGLTTVLI
                     DAIPLLSAVQQWPRSTLEILPSGAVPPNPAELLASERMKELIQELTDEYDLVVIDTAP
                     VQVVADATVIAQQVDSVLIVADSTRVRRAQLANTIETLERSGAHISGVVLNRVKPRSR
                     DDYYYVQDEAEETPGTGIPLPRRHAKAGSDRPAHPTESLEPK"
BASE COUNT         1628 a         3341 c         3290 g         1552 t
ORIGIN      
        1 cggcgcgacc tcgtccggcg tgaactcgtc gcacacgtac gcggccgcgg gaagctaccc
       61 gatcgccctc acggtgacgg acgatgacgg cgcgaccgga tcgacgatct cgacggtcac
      121 ggtcaccagc ccgggcggtc catccgtcct ggcgtccgac gccttccagc gtgaagtgat
      181 caacggcctg ggcacggcgg atgtcggcgg accatggacc acgtctggag gcagcaccgc
      241 cttcagcgtc acgggcggta ccggacggat ccggatggcg acccccggcg gaacgctgaa
      301 cgcatacctc gatggtgccc cgcagcaggc gggagacctg cgtctcagct tcgcctcgga
      361 caagcccgcc accggcagtg gcgtctacgt ctcggtgatc gggcggcgag tggctgcgat
      421 cggcgcttat caggcgaaga tcgtgctccg tgcaggcggc accgtcggca tctcgctcgt
      481 gcgcgtcaac gcgagcggag gggcagaggt ggcgctgcag gcagcgatca acgttccggg
      541 cctcgcctat gtcgcgggcg atcggttgaa cgtgcgtctg caggtcgtcg gcgcatcgcc
      601 gacgacgctc aacctcaagg tgtggaaggc cggcaccacc gagccgtcag cttggcagcg
      661 cacagccacc gactcaacgg ccggattgca gaccgcaggg ggcgtggggc ttcgcgccta
      721 cctctccagt acgagcacca atgccccggt aatcgcctcg ttcgacgacc ttgtcctcac
      781 ggtcccctag ccgctgttct ccgtggcctt ctgggagttg cagaacagcc acacatcaca
      841 ggcgcgcagt gaggagctgt gaagccggct cagacgaggc ggcgcgctca tgagagttct
      901 cttcgatgcg tactggtggg aatcgggccc gatcagcaac cgaatggttc agacggaact
      961 catcaacgca tggtcgcgaa cgttcccaca agaccagatc ggtctggtgg ttcctggtag
     1021 gccccgaccg ttgcgggctc tcccgcccca tgctgaagtc atgttctcgc gctcctcatt
     1081 tctccaggcg cccatgaacg ggttgctcat ccccagaatc gccaagcgct ggggagctga
     1141 tgtatcagtg gtgcacaatt tctcggccct tcggggctac tctgcgacat tcattcacga
     1201 tttcatgttc atggatcacg cgcggtggtt cacatgtatc gagaacgcgt acttccgcct
     1261 catggttgca acttcacggc gcgcaagcct cctcctgacg tcgagtcaga ccgaggcgaa
     1321 ccgggtcttt cgaatctctg ggcgcgaagc cacgccgatc ggtctcggac ttcgcagcgc
     1381 gttggttgag tccgagccaa cccgaccgcg agcagtcgcg acagaggggc aggcgtttgt
     1441 gatggctgtc gggcggttga atgcccggaa gaaccttgcg atggcaatcg agggctacct
     1501 ggcttcgggc ttgaacgtgg atacccgctt ggtcgtcgtg ggtgaacgat ccgggcgatc
     1561 tgagctcctc tcccgccccg cgctggacgc gatcgagggc ggcctcgtgc gtctcatcgg
     1621 atccgtgtcg gacggcgagc tgcgttggct ctacgagcac gcgagagcat ttctcatgct
     1681 ctcgctggat gaagggttcg ggctcccgtt ggcggaagct cgatacttcg gtaccccagc
     1741 agtcgtcagt gatctccccg tgttccacga ggtgctcggt gactacggga ccttcgtgaa
     1801 tcccaccgac cctactgata ttgcccgcgg catactcggc gtgcgccggg gctctaccaa
     1861 aggggagcgg catcaatggg aggactccgt gcggatcatg cgtagcaaga tcgcggaacg
     1921 gctcaggtca tgaagcgcaa tgaggacggt gctgaggccg accggctcgc gctggtcgtc
     1981 gtgaacttcg cttcctcgtc gctgctcgcg gcgaacctcg tcgatcccgg tcttcgaggg
     2041 gccatcggat gcatcgtggt cgtcgacaat ccgagcacgg ccgacgagac gcggcgagtg
     2101 cgggagctgt gcgtcacgaa cgactgggcc ttggtcgagc tggcacgcaa cgagggcttc
     2161 ggcgcgggcg tgaatgccgg cgtcgcacgc gcccgccagc tcgggtgcac gcgggtgctc
     2221 gcactcaatc ccgacgcgac gatcgacgcc gattcggcgc gggagctcct cgcggcgagc
     2281 agggccgacc cgcgcgcgct catcgggccg cacatcgtcc gacctgatgc aacggtctgg
     2341 ttcgcgggtg cggtgctgga cccgcgggcc ggcaccactc ggcgagcgcg cgaggaggag
     2401 ctcgatggtg agacgacctg gcagaccggg gcctgcttcc tggccacgat cacgatgtgg
     2461 gacgaagtcg gcgggttcga cgacgactac ttcatgtact gggaggacat cgatctgtca
     2521 tggcgttggc gcgaagccgg agggaggctg atcgtccacg agaacgcgac ggccgttcat
     2581 gacgccggcg gcacccaagc cggttccggc aaatcccccc tctacgtcta cttcaactgc
     2641 cggaaccgcc tgctgttcgc ccgcaagcgg ttcaggtcga gatacgggcg gagatggtgg
     2701 cggggcagca tctcctacgg ctggagcgtc gccacgcgag gaagtcggag gatgctcgcg
     2761 aagcatccgc tgctgctgtg gtcggcgctc aggggaacgc tcgccggcgc gttcgggagc
     2821 ctcactccgc ggtacgcgtc ggcacgtcgc tgatgacgta cttcgtcttc gcgccggtgt
     2881 gcggcagcga atcgacgtac tcgagcgtga ccggcacctc cccggcgacc cgccggcgga
     2941 ggatctccac ttcgtcttcg atgtgacgcg tggcattcgt accagtcccg agtaccacgc
     3001 ggacggtgat cgagtagtcc gcgttttggc gaatctggaa gagccgcacc gactcggggt
     3061 gctttgcgaa catcaccgtg aggcgggtga cggccatgcc gctgggcaac cgcagcacgt
     3121 cggtgatcct tccgtctggc tgcgccatgc ggggaagcga caccccgcac gcgcaggtcc
     3181 cgtcgatgag ggagccgcga tcaccgagcc ggtaccggat gatcgggaag acccgattgg
     3241 cgagatcggt cacgacgatg tcgccgacct cgcccgcggg cagcggcctc ccgttcgtgt
     3301 cgaccacctc gatcttcttc gcgtctgcga agatgtgcag gccgtcctgc attcggcact
     3361 ctccggccgc ccacgacatt tcggcgccgc ggtactcgtc gtacacgggg gcacccagca
     3421 ccgactcgat gcgtcgccgt gtcgatgggg ggagcggcga cgccgttgtc gcgatcgcga
     3481 tgggggtcgg aatggtcagt ccctcggcct cgaggaagtc cgcaaagtcg agcacaccac
     3541 ccacatagcc ctcgatgagc gcgggacgtg tgcgaacgat ggcgcggtgg aattcccgca
     3601 tcgagtcggc gtccatgagg ctggcatcga ggtagagctg cttcgacggc caccattgaa
     3661 gacggttgcg cagcgtgtcg aagcgcccga aaccccaacg cccgagccgg gccaggtcgt
     3721 cccacggctg cacgccccac cagctgtaca tgcgccaggc gagcgcgagc gacgggaccc
     3781 gggaatcttg tgcggtgcgg agcggtacac cggtgctgcc gcccgtgagc gcctcgcgcg
     3841 cgaagcgttc gctgttcgcc ggccgcagct ctgcgtcatg ctccttcagc tgagcacgct
     3901 cggtgatcgg gatgcgctcc cactccgcgg ggtcgtcgag tcgggcggca tcgatgccat
     3961 gatcagcaaa caggcggcgg tagtaccccg tcgattcggc tgcgaaacgg gcgatcgagg
     4021 cggcccggtg cgactgcagt cgcgccagtt cgtcggcact caggtgctgg ttgcgcagaa
     4081 gctcggccag gtactcgccc gacgatcgcc gcacggtgcg gctcttgacg gtgaaggacc
     4141 actgcttcag cctcatgccg ccagcatagg tgcgatgacc ggactgcggg tcggacggcg
     4201 tcgtcgatga ccgccgccgc ccgtactcgt cgtgtgcgta tccgaccgcg ccggcgatca
     4261 tcccgaagcc ccgactcgcg atgcgcaacc cgtgcgcccg gtggtgaggc gaacgcgaca
     4321 ccatcccgaa ggcccagcgc atgctcccgc ccacggcgcg cgttgctccc cgacccaatg
     4381 ccagcacccg ctcgacgagt ctgagggcag cggtcggggc gagcgcgatc cgcacccgcg
     4441 cggaggtgtt gccgaaccgg aagacacgca tcagaatcca cgaccgcgtg tgacgtgccg
     4501 gatcgtcctg ctcgaccacg acggcattct cggcccagcg gatcgtgccg ccgcctcgcg
     4561 tgagctgcag cgtgaacaag ctgtcgctgc caccggtgag cccgaatcgt tcgtcgaacg
     4621 acagcccgag tcggcgtatc tggtcgaggt cgaggaggag attgttcgtg gccgcctcgc
     4681 tcatcagctg accgtgggta cgggtggggc gttcgaacgc gttgcgcgag ccccagcgct
     4741 cggcggtgtc gggcaggcgc gtcaccacac gaccggcgac agcggtcgcc cctgtcgtca
     4801 tgtacatcgc gatcatggcg cgaagccacc cgggttcggg ggtctcgtcg tcgtcgatga
     4861 aaagcaggac gccgccgttc gcgcactcct cgagcgcccg gttgcggacc gcggcgatcc
     4921 ccggccgcgg ttcgacgacg taccgtgcgc cgtgctcggc ggcaatgcgc tcgccggagc
     4981 cggcggggtc attgtcgatc accaggatcg agcagcgcat cggggccgcg ggcagcgcgt
     5041 tctcggcgtc gacctgctcc cgaaccgcgc tgagcgcgtt ggcgagcgcc tcagaccgcc
     5101 ggaacgtcgg gatcgcgacg gtgagctcgg atacgtggcc ccgcgtcggc gtcggcgcgg
     5161 acgcgacggg agatcggccc ctgcgacggt caatggccag ctggtaggcg cgggcgtggc
     5221 gggcgccgac gtcgtgccac ccgcgagcgc tgaggtcggg agccgctcgg ggcggctcgg
     5281 cccgtaccaa ctgaatcgcg tcgagcagca tgccggcggt gagctcaccg tcgaacaggt
     5341 gcacccagcc cgcgcccacc tcggctgaca gtgcgcgatt gaggtcgttg ccgggcacga
     5401 gaatggggcg cccgagcgag agcgccgcca aggcggttcc cgagttgtgc atgaaccggt
     5461 acggcaagac gacgagttcg ctcgacgtga ccacatcgac gaacgcctgc tcactgacgt
     5521 aacgcagtgt cgtgtcgagc ccactgagtc gttcggtcgc ggtgagcagt cggcgctcga
     5581 tcgcctcgtt cgcaggtttg cccgagattc ggagggtgag ggtcggatcg attgtggatg
     5641 cctcggcgaa tgcgtcgacg agccgctcga cccccttgta gggcttgatc agtcctgcat
     5701 acccgagtcg tccgggaacg gtcgcggcac gcggcatccc gtcgaaccag gttctatagt
     5761 gcccgtgcgg aatcagcacc gacggaaccc ccgcaacctc tggggtcacg gcggcgatgc
     5821 gaattcgcac gtcggtgtgc cgctcgagcg cccggatgag ggcacgatcc aggaacgggc
     5881 cagacggcgg tgtgatgttg tggacggtgc gcacgacggc gacccggcgc agccgcaccc
     5941 gtgtgacaag acacgccagg gcgacccgct tgccagccct cgtccaccag tggcgtgctg
     6001 ccagcagcgc gtcgggccag tggatgtgca tcacgtcgta gcgcccggtc agggccgcac
     6061 gccatgagaa gggaatgtgc tcgaccgccg gctcctcgat gagcgcggcc ctcagcatga
     6121 cgatgtacgg attcgtggtg ccccgcggtt caccgaaggt ctgcatcact cgcattcggc
     6181 cccctctccg cgtctgcgcc gtcgcggcat ccgatcgccc gctgccgccg cccggtcgtc
     6241 gaggcgtccc gcgcagcgaa agcgagacgt actcgattct cacaggttga ggccgttaag
     6301 atccatcgga tacctgaaca gcagtcagcc gacaggcccc tcacgtaggt ggcgccgaac
     6361 ccgatctcag aaactccggt atgaagcaca agaagcagaa gcacaagaag cagcagacca
     6421 cgaagaagaa gttcgcactc gtcgcactcc tcaccgcctc gatcgcgctc gcgccgatcg
     6481 cggcgctcac cgctgtcgcc cccgcatcgg cgaccaccgt ctccagcccc tccaagacgc
     6541 tgcagcccgg gtcttcgtac gtgacgcggg ccgccggctc ctggacggac ggaaccgcca
     6601 ccctgtcgac gaagttcgcc gcagtgccca cgcgcggcgg cggcctgtac accggcgtcc
     6661 gagttcggga atcgaacggc agctactacc tcgcccacac ccggacgtat ccggatggca
     6721 cggtccaggc ctccatccgc cgcatcgtcc agaccaacgg cacgtcggcc gagaccctcc
     6781 tctcgacccg cgaatccgta gcaacgaagg ccaaggccgg atccgcgttc tcgatcaggt
     6841 tgcaggccga gggcaccgac acggtcaagc tccgcgcgac aataaccgtg ggcagcggca
     6901 cgccgctcgt gctcaccgcg accgactcga gctcgtcggc cgtcgaccgt gccggcaccg
     6961 cggccggatg gggctatctc tcgacgtcca cggcgcggat cgccatcgct cccgcgacgg
     7021 tcgagtcgac tccgaccgcc gcaaccgcta cgccgacgcc gacgccgacg ccgacgccta
     7081 ctcctacgcc gactcctacg ccgacacccg ctccgacgcc gactcccacg ccgacccccg
     7141 ctccgacgcc ggctccgacg ccgggccctg caccgtcgaa cccgtccccg gctccgggtg
     7201 cgtacccgag cgccgagacg accggtgttc ccgcgggcac gaagctcacg gtgcaccagg
     7261 gcgacctcac cgtgacgacg gccggcgcgg tcatcgacgg cgttgaggta cacggcgtgc
     7321 tcaccgtgaa ggcaccgaac gtcgtgatca agaactcccg cattctcggc cgcgcggtcg
     7381 acaaccacat cggtctcgtc aacaacatcg tctccggcgc cccgttcacg atccaggact
     7441 ccgagatcta cgcggcgaag ccgtcggcct ggcagaacgg catcttcggg agtaacttca
     7501 ccgcgacgcg cgtcgaaatc catggagtga tcgacccgat caaggtgatc ggcgataacg
     7561 tcgaggtccg cgattcgtgg ctccacgaca acctccacta cgagcaggat gagtcgcgag
     7621 gcggaactcc gagccacgac gactcgatcc agatcgaggc cggcaagaac atctcgatca
     7681 ccgggaaccg gatggagggc gccgacaacg cggcgatcca gatcacgcag aacacgtcca
     7741 agacgccgct cggcaacgtc cgcatcagcg acaactcctt caacgacggc ggctgcaccg
     7801 tgaacatcgc gaagacgccg acgtcgatcg gcgacttcgt cgtgacggac aacgtattcg
     7861 gccccgaccg caagttcgcc aagtgcggca tcctcgcgcc cgccatgaac acgccccagc
     7921 agtcgggcaa cacctgggag gccactggaa ccgcggtcgt ccagacggtg ctcaagtaac
     7981 caccgcggca cgcggcatcc gtcaaatgcg gcccgggctt cgaagctcgg gccgcagcgg
     8041 cgttccggcc gtcggctcgc cctggacctc gaagcgcgtc tccggcgccg acgtcgacgt
     8101 ctcgaggccg acggcgttac ccgacaggaa tcgggccagt tcggggaagc tcgagtagtg
     8161 cggtccgagg atgtggcacg agccggcgag caggtacagg tcggcgacgg cggcctgcag
     8221 cgccggacga ctgttgtacg cgcccttgtc ggtctgcccg acgcaatcgg ggaatcggcg
     8281 ggcgacctgc acgaatgcgg catccgtgtc ggccgcgacg tagagcccga gctcgggccg
     8341 ttccgaacgg atctcctgga tgcgcgaaag gtaccactcc accggtgact cgcgcagggt
     8401 cgcatcgtgc gcgaccgaat gcgttcggat catgacgccg aggtaggggc gatccgcgga
     8461 tgccgcggcg agctccagga tggtacgccg gatcccctcg acgggctgga gtgcccggag
     8521 ggccgcatgc catgagggtg cgcccggcgg cagggtgagg gcgtgcgggg tgcggatctg
     8581 ccacacacgt acctgccgcg cagcgtcgtc gatccacgtg aggcccgcac catggtacgg
     8641 gtacgccttc gcgaccatcc gcgagcggat cgtcgtccag cgtcgccctc gaaactccca
     8701 gagctcgtcg agccgcgccc cgaattcacg ccctgtcggc cagacgtagg cgaacgatct
     8761 cccctcgtac ctggcgagcg cctgcgcgcc gagtacgacc cgcatgcgat tcccgagccc
     8821 gtggtagcgc cccgtgacgg cgaccacgag cccctcactt cggttcaagg ctctccgtcg
     8881 ggtgtgccgg ccggtcgctg cccgcctttg cgtgtcggcg cgggagcggg atgcccgtgc
     8941 ccggtgtctc ttcggcttcg tcctgcacgt agtagtagtc gtcgcgcgac cgaggcttca
     9001 cgcggttgag cacgacgccg gagatgtgcg ccccggagcg ttcgagcgtc tcgatcgtgt
     9061 tggcgagctg ggcgcgccgc acgcgcgtgg agtcggcgac gatcagcacc gagtccacct
     9121 gctgagcgat caccgtcgcg tccgcgacca cctggaccgg cgcggtgtcg atcacgacga
     9181 ggtcgtactc gtcggtgagc tcctggatga gctccttcat ccgctccgac gccaggagct
     9241 cagcgggatt cggcggtacc gcccccgagg gcaggatctc gagcgtggat cgcggccact
     9301 gctgcacggc gctcagcagc gggatggcgt cgatcagcac cgtcgtgagc ccgactgctc
     9361 cgacgatgcc gaacgcgtcc gcaaccttcg gcttgcgaag gtcggcgtcg accagcagga
     9421 cccgctttcc cgtctccgcg aggaccagcg ccaggttccc ggcagtccag gtctttccct
     9481 cggcgggacg cgacgacgtg atggcgaggc tccgcgtctc gtggctcgcc gacgcgaatc
     9541 ggagtccgga tcgaacatgg cggaaggact ccgccgcact cccattcggc tggcggatcg
     9601 ccaccggtcg aggatccgag gacgacggcg tctgctcgat cgacccgagg acggggagtt
     9661 ccgtgatcga cttcagcgtc gcctccgtgc gcacgcgggt atcgagtgcg ctcgcgagca
     9721 cgacggcgaa cgacgagagg aggaaaccga tgatcgcccc gagcatcgcg tcctgctgct
     9781 tgttcggcga gctctggaat atcgccggag t
//
LOCUS       DATDBZ010000577        10505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1836495, whole genome shotgun sequence.
ACCESSION   DATDBZ010000577 DATDBZ010000000
VERSION     DATDBZ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836495"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..605)
                     /locus_tag="VIX62_13305"
     CDS             complement(96..605)
                     /locus_tag="VIX62_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY5861253.1"
                     /translation="MFRKGDTVVHPEHGAAVIEELREKEFLGEKRTYLVLRVAYGDLT
                     LMIPTDSTEQVGLRSVVSKAEVKKVLKVLTEDESSMAANWSRRFKNNMEKLHSGDPYQ
                     VAEVLRNLSIRDREKGLSAGEKRMITKARQILISELSHATGKSEDDAEIMIDDVLDGA
                     HGVKVVAQA"
     gene            complement(1016..2335)
                     /locus_tag="VIX62_13310"
     CDS             complement(1016..2335)
                     /locus_tag="VIX62_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY5861254.1"
                     /translation="MAKTRTIMACRDCRQQVSQWAGRCPGCGAWGTIEEVAGASRDGR
                     SSTPVPLRREAGMSVDARVTTGLGDLDRVLGGGLVPGSVTLLAGEPGIGKSTLLLQLL
                     AGVAADGHPCLLVSGEESHAQVAGRTRRLGIDGDQISFAPGRDLAAVLSTATEACPFL
                     LAVDSIQALRDTSGSQVPGGVSQVRTCADALVGLAKTLDIAVVMTGHVTKDGDLAGPR
                     ALEHAVDVVLSFDGDARSGLRVVSGGKNRFGAEGESAWFEMGAGGLAPIDPTAILVSG
                     QQVPGAAVGVPLAGRRALAVEVQALVGSTEGPARRQATGLDARRFQLVAAVLDRSTGI
                     RLGRAELFGASSGGIRVEDPACDLAVAAALASASSGTCAPEGAVFIGEVSLTGQVRSV
                     VGMRQRFAAARAAGCTHVFALPSDEASDGLQVVPVRSVAEAVSWSQA"
     gene            2583..3356
                     /locus_tag="VIX62_13315"
     CDS             2583..3356
                     /locus_tag="VIX62_13315"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEY5861255.1"
                     /translation="MHDANPEPSPFDHFLDEGLIEHVIRPIKSGKEASVHLCRANPRT
                     TGHDLLALKAYHPLNRRDFRDESMYRDGEWIKPRRVRVALEKKTTFGRQVQGSIWVNR
                     EWETLRALSDGGVSVPAPIERTEDAILMTYVGDEELAAPQLRNYRPDDTAEAQDLFDQ
                     LLRAIERMLYLNVVHGDLSPYNLLVWEGTLVVIDVPQAVDPRKNRHARSFLERDLERA
                     CGWAEAHGLGADESRLTADYWTAWEFADLIPEDLRGLTM"
     gene            complement(3372..4184)
                     /locus_tag="VIX62_13320"
     CDS             complement(3372..4184)
                     /locus_tag="VIX62_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861256.1"
                     /translation="MRTAMKVLAWLGTVLAAAGIVGGAYLYGVNAGLFAAGRADDVGR
                     RLLADQLFQRVVAERTADAIVEQAPALEPRREAIVESAVAVTETRAYRELFQGVVDIA
                     YERALDDELDEPVVITAGDLARLLTREEAVIGALPLGPLSSEGVELMSARDIVKLRKA
                     QSAAERWDTPLLAGGIVLAIVAIALPGRKGPRLVLLGACIALGAAVLYFANDIAGDEV
                     AARSPRDAAAVVDALWGSAATSVRVWLGGTFMAGLLVVATGFVIGVSRDTTA"
     gene            complement(4396..6891)
                     /locus_tag="VIX62_13325"
     CDS             complement(4396..6891)
                     /locus_tag="VIX62_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY5861257.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLNISLEAVRQQVEEIIGQGQAAPTGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQKLGADLNRVRQTVIQLLSGYTGGKGETTPGGQGEG
                     QSGQGSMVLDQFGRNLTQLARDGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEPGV
                     GKTAVVEGLSQDIVRGEVPETLKGKQIYTLDLGALVAGSRYRGDFEERLKKVLKEIKT
                     RGDIVLFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTRDEYRKHLEKDA
                     ALERRFQPIEVQEPSVAHTIEILKGLRDRYEAHHRVNFTDDALVAAANLADRYISDRF
                     LPDKAIDLIDEAGSRMRIRRMTAPPNVREVDEKIAEVRLEKESAIDGQDFEKAASLRD
                     KERQLQEERSRREEEWKSGEMDILSEVDEEEIAEVLSIWTGIPVFKLTEEETEKLLRM
                     EDELHKRIVTQNEAISAVSRAMRRTRSGLKDPRRPAGSFIFLGPSGVGKTELSKALAE
                     FLFGDEDSLIQLDMSEYMEKHTVSRLIGSPPGYVGYEEGGQLTEAVRRKPFSVVLFDE
                     IEKAHPDVFNTLLQILEDGHLTDSQGHKVDFKNTIIIMTSNLGTRDIQKGPGIGFAAS
                     ANEEVTYEKMRDRVTEELKKSFRPEFINRIDEVIVFRSLTQDDVKNIVDLMMRRVRDQ
                     LKAKDVEIELTEAAKVLLATQGYDQSLGARPLRRTIQRLVEDPLSEKLLYKEFRAGET
                     IFVDAVDGEIVFEHTDGMLPPDVPPVELAGSTE"
     gene            7417..7800
                     /locus_tag="VIX62_13330"
     CDS             7417..7800
                     /locus_tag="VIX62_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5861258.1"
                     /translation="MSLRVLVVDDHPAFRKALTSALRMVADIEVAGEAGGGIAACEEA
                     ETLEPDIVLMDLSMPDLSGIDAMKKIHESRPGLPVVILTAHADEGVEREAREAGARGF
                     LAKGTGLQDLVITLHEAVGEDEPVL"
     gene            complement(7825..9405)
                     /gene="lysS"
                     /locus_tag="VIX62_13335"
     CDS             complement(7825..9405)
                     /gene="lysS"
                     /locus_tag="VIX62_13335"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY5861259.1"
                     /translation="MSEHDDESAEPIEEDEPTAEDEQGSSREAEILAARRESLARLTD
                     AGMDPFALTLETALGVAEPDAIAGIHREFGDLAAGTTGDGIRTVAGRIVQRRDMGKLK
                     FLVVRDRTGDIQLFCNAKAMDPVAFALLDEADLGDIVAASGPVGMTKKGELSIFVERW
                     AMLTKSLRPLPEKWHGLKDPDLQQRRRYLHLATDDVPRRYFLARAAVLQTMRRVLDER
                     GFVEFEGPMLQLVAGGANARPFTTFHHVLDQEMKLRISLELYLKRMLVGGVERVYELG
                     RNFRNEGIDRDHNPEFTMLEAYQAYGDYRSMMELSETLLRESARAVNPIVGRPEDDLR
                     IGYRDLTIDLEPAFRRITILGSVSEATGEEITLDRPDLADIARRHEVHLEDGWGPGKI
                     VQELFEKLVEDTIEQPTFVCDFPREVSPLARPHRDDPRLTEHFDLVVGGLELVTAFSE
                     LTDPIEQREKFELQAAAKAAGDDEAHPLDEEFLRALEHGMPPAGGLGMGIDRTLMLLT
                     DAPSLRDLIMFPSQRPEE"
     gene            complement(9398..10177)
                     /locus_tag="VIX62_13340"
     CDS             complement(9398..10177)
                     /locus_tag="VIX62_13340"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY5861260.1"
                     /translation="MLLAADVGNTEIVLGVFRGPELEHTWRLSTQPERTPDELALALS
                     GFLAQRGMSFESQVTGVVMASVVPDVTAAVREMAARYFPFPPVIVGPGVKTGVPILTD
                     NPREVGADRVVNALAAFTRFGGPAIVVDFGTATNFDVVSAKGEFIGGVIAPGMQASAA
                     SLFSRTARLTRVELTAPPSVVGKSTVEAIQSGLIFGTAGEVDAIVTRIRAELPGATTI
                     ATGGLASTVIPHCTTIDHHEPSLTLEGLRLVFEKNVVTADE"
     gene            complement(10196..>10505)
                     /locus_tag="VIX62_13345"
     CDS             complement(10196..>10505)
                     /locus_tag="VIX62_13345"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861261.1"
                     /translation="GILAGMPVSKEAFGRMGVRCRASVSEGTPIVDGDQVAELGGPVA
                     AMRGAAPTALRFLTSLSEVASGRREPEPGDPFGDYALALRLSGQDPVGHDGPSFALEI
                     "
BASE COUNT         1629 a         3581 c         3577 g         1718 t
ORIGIN      
        1 aggccgaacg agccctacgc cgtggtcatc ggcgcccagg ccgggtgcct gtagggatgg
       61 gatgcgttgg tggagaactc aggagtggcg aacgatcagg cctgcgcgac gaccttgacc
      121 ccgtgggcgc cgtccaagac gtcgtcgatc atgatctcgg cgtcgtcctc ggacttgccc
      181 gtcgcgtgcg agagctcgga gatcaggatc tggcgtgcct tcgtgatcat ccgcttctcg
      241 ccggccgaaa ggcccttctc gcggtcacgg atggacagat tcctcagcac ctcggccacc
      301 tgatacgggt cgccggagtg gagcttctcc atgttgttct tgaagcggcg gctccagttg
      361 gcggccatgg aggactcgtc ctcggtgaga accttgagca ccttcttgac ctcggccttg
      421 ctgacgaccg accgcagccc gacctgctcg gtggaatcgg tggggatcat cagggtgagg
      481 tcgccgtagg cgacgcgcag cacgaggtac gtacgcttct cgcccaggaa ctccttctcc
      541 cgcagctcct cgatcactgc ggctccatgc tccggatgca ctacggtgtc gcccttacgg
      601 aacacggaac ctcccagatc ggtggaaaca ggggcctggc tgactagtca aagggtagca
      661 tgagagggtc ttgcgacgca acccatcacc gcaggtcaga ggccgtattc gtcgcttgat
      721 cggccgccgg agacctaggg cgaacggcgt atcagcgccc tcgttcccac ccggagaacg
      781 gaccggggga acccccgaac ctgcctccgt gccacgccgc cgccgttcgt gtgctgtggt
      841 ttgacacggc tccggcccgg gcgcataaag gctacgttcg cgtatcgcac gattccgcga
      901 ggaagctccg atctcgcgaa ccaacgcggc ggccggcgac ggtcgtcctc ggctcgaagc
      961 gcccctcgat ccgagggcgg cacgccggtc gtccgctgct ccccaggggt gatccctagg
     1021 cctgtgacca gctcacggcc tcggcaaccg agcggaccgg cacgacctgg aggccgtcgg
     1081 atgcttcgtc gctaggaagc gcgaacacgt gggtgcatcc cgccgcccga gctgcggcga
     1141 accgctggcg catgccgacc acggagcgca cctggcccgt gagggagacc tccccgatga
     1201 acaccgcccc ttcgggggca cacgtgccgc tcgacgccga tgccagcgcg gccgccacgg
     1261 cgaggtcaca cgcggggtcc tcgaccctga tgccgcccga ggacgcgccg aagagctcgg
     1321 cgcgtccgag ccggatcccg gtggaacggt cgagcacggc ggcgaccagc tggaaccgtc
     1381 gtgcatcgag accggtggcc tggcgccggg cggggccctc ggtgctgccg accagggcct
     1441 gcacctcgac cgcgagggct cggcggccag ccaggggcac cccgacggcg gccccgggaa
     1501 cctgctggcc ggagaccagg atggccgtgg gatcgatcgg cgcgaggccg ccggcaccca
     1561 tctcgaacca cgcgctctca ccttcggcgc cgaaccggtt cttgccgccg ctgaccacac
     1621 ggagcccgga acgtgcgtcg ccatcgaacg agaggacgac atcgacggca tgctccaggg
     1681 ctcgtggccc ggcgaggtcg ccgtccttgg tgacgtggcc ggtcatgacc acggcgatgt
     1741 cgagcgtctt ggccagcccc accagcgcgt cggcgcacgt ccgcacctgt gagacaccac
     1801 cgggcacctg ggagccggag gtgtcgcgaa gggcctggat ggagtccacc gcgagcagga
     1861 acggacacgc ctcggtggcc gtggacagga ccgccgcgag gtcgcgaccg ggggcgaacg
     1921 agatctggtc accgtcgatc cccaaccgac gggtgcggcc ggcgacctgg gcgtgggatt
     1981 cctcgcccga gaccaggagg cagggatggc cgtcggcggc gacccccgcg aggagttgga
     2041 gcagcagcgt cgacttaccg atccccggtt cgcccgcgag cagggtgacc gacccgggga
     2101 cgagccctcc gcccaggacc cgatcgagat cgcccagtcc cgtggtgacc cgggcgtcga
     2161 cagacatccc ggcttcacga cggagcggta ccggggtcga gctgcgcccg tcccgtgagg
     2221 cccccgcgac ctcttcgatc gtgccccagg ctccgcaccc ggggcaccgg ccggcccact
     2281 gggagacctg ctgacggcaa tcgcggcacg ccatgatcgt tcgtgtcttc gccatggccc
     2341 gaccctatcc agcgggaccg acacggttcg aggtgctagg gtggatccac tcgcgacgtg
     2401 atcgacgcgt cgcccccgac cggaaggagt gatgagcgat gactgcaacc tcggtcctcg
     2461 acatctcgca cacgctgcgt ccttccgggt cctgcgcgta gccggcgtct ccgccgctcg
     2521 cgccccgctc ccgtccggac gcggcaccgc tgtgtgtcca cgatccgaaa ggagcacccg
     2581 cgttgcacga cgcgaacccc gaaccgagtc cgttcgacca cttcctggat gaagggctga
     2641 tcgagcacgt catccgaccg atcaagagcg gcaaggaagc atcggtccac ctgtgccgcg
     2701 ccaacccacg gaccaccggc cacgatctgt tggccctgaa ggcatatcac ccgctcaacc
     2761 ggcgagattt ccgcgatgag tcgatgtacc gcgacggcga atggatcaag ccgcggaggg
     2821 tccgcgtggc cctcgagaag aagaccacgt tcgggcgcca ggtgcagggc tcgatctggg
     2881 tcaaccggga gtgggagacg ctccgcgccc tgtccgacgg cggcgtatcc gtccccgcac
     2941 cgatcgaacg aaccgaggac gcgatcctca tgacctacgt cggcgacgag gaactcgccg
     3001 cgccgcagtt gcggaactac cggcccgacg atacggccga ggcgcaggat ctgttcgacc
     3061 agctcctgcg ggccatcgag cggatgctct acctcaacgt ggtgcacggt gacctctcgc
     3121 cgtacaacct gctggtgtgg gagggcaccc tcgtggtcat cgacgtaccg caggcggtgg
     3181 acccccgcaa gaaccgccac gcgcgttcgt tcttggagcg agacctcgag cgtgcgtgcg
     3241 gatgggccga ggcgcatggt ctcggcgccg acgagagccg gctcaccgcc gactactgga
     3301 ccgcctggga gttcgccgat ctcatccccg aggacctgcg aggactcacc atgtgactgc
     3361 ggtcgcctga ctcaggcggt cgtgtccctg ctcacgccga tcacgaaccc ggtggcgacc
     3421 acgaggaggc ccgccatgaa ggttcctccg agccagacgc gaaccgacgt cgcggccgat
     3481 ccccagagcg catcgaccac ggcggcggca tcccggggcg aacgagcggc gacctcgtcg
     3541 ccggcgatgt cgttcgcgaa gtacagcacc gcggctccca gggcgatgca ggcgcccagc
     3601 aacaccaacc ggggcccctt gcgcccgggg agcgcgatgg cgacgatcgc gagcacgatg
     3661 cctccggcca ggagcggcgt gtcccaccgt tcggcggcag actgcgcctt ccgcagtttc
     3721 acgatgtcgc gggcgctcat cagctcgacc ccttcggagc tgagcggacc gagcggcagg
     3781 gccccgatca ccgcctcctc gcgggtgagg aggcgggcca ggtcgccggc cgtgatgacc
     3841 acaggttcgt ccagctcgtc gtcgagcgcc cgttcgtagg cgatgtcgac cacgccctgg
     3901 aacagttccc gatacgcacg ggtctcggtg accgcgacgg ccgactcgac gatcgcctca
     3961 cgcctgggct cgagggccgg cgcctgctcc acgatggcgt cggcggtgcg ctcggccacc
     4021 acccgctgga acagctggtc cgccagcaat cgacgcccca cgtcgtcggc gcgtccggcg
     4081 gcgaagaggc ccgcgttcac cccgtagagg taggcaccac cgacgatccc ggcggcggcc
     4141 agcacggtgc ccagccaggc caggaccttc atggcggttc gcacgtcggc atcctacggc
     4201 tgcggaacgt gccgactacg gaccggcggg gtcgtccgcg atcgggttcg gcgatggagg
     4261 gacgaggaac cggtcgggat ccggacccag cacgacgcac gctggatgcc gagaagcccg
     4321 gtccccatgc ggggccgggc ttcggccttg agtggtggcg cctcacagga cgccgccggg
     4381 atgcgggtga tcgacctact cggtcgaacc cgcaagttcc acgggaggca cgtcgggcgg
     4441 aagcatgccg tccgtgtgct cgaagacgat ctcgccgtcc acggcgtcga cgaagatggt
     4501 ctcgccggcg cggaactcct tgtagaggag cttctcggag agcgggtctt cgaccagacg
     4561 ctggatggtg cggcgcagcg gccgggcccc gagtgactgg tcgtagccct gggtggccag
     4621 caggaccttg gccgcctcgg tgagctcgat ctcgacgtcc ttggccttca actggtcgcg
     4681 cacccggcgc atcatcaggt cgacgatgtt cttgacgtcg tcctgggtca gcgagcggaa
     4741 gacgatgacc tcgtcgatgc ggttgatgaa ctcgggacgg aaggacttct tcagttcctc
     4801 cgtgacccgg tcgcgcatct tctcgtaggt gacctcttcg ttggcgctgg ccgcgaaccc
     4861 gatgccggga cccttctgga tgtcacgcgt gcccaggttc gaggtcatga tgatgatcgt
     4921 gttcttgaag tccaccttgt gaccctgtga gtccgtgagg tgaccgtcct ccaggatctg
     4981 gagcagcgta ttgaacacgt cggggtgagc cttctcgatc tcgtcgaaca gaaccacgct
     5041 gaacggcttg cggcgcacgg cctcggtgag ctgaccacct tcctcgtacc cgacgtagcc
     5101 gggaggcgag ccgatgaggc ggctgaccgt gtgcttctcc atgtactcgg acatgtcgag
     5161 ctggatcagc gagtcttcgt caccgaacag gaactcggcg agcgccttgg acagctcggt
     5221 cttgccgacg cccgagggcc ccaggaagat gaacgagccg gcgggacgac gggggtcctt
     5281 cagcccggac cgagtgcggc gcatggcgcg ggacaccgcc gagatcgcct cgttctgggt
     5341 gacgatgcgc ttgtggagct cgtcctccat gcggagcagc ttctcggtct cttcttccgt
     5401 gagcttgaag acggggatgc cggtccagat ggacaggacc tccgcgatct cctcttcgtc
     5461 gacctcggac aggatgtcca tctcgccgga cttccactct tcctcgcgac gggagcgctc
     5521 ttcctgcaac tgtcgctcct tgtcgcgcaa ggaggcggcc ttctcgaagt cctggccgtc
     5581 gatcgcggat tccttctcga ggcgcacctc ggcgatcttc tcgtcgacct cgcgaacgtt
     5641 gggcggcgcg gtcatgcgac ggatgcgcat gcgcgagccg gcctcgtcga tgaggtcgat
     5701 cgccttgtcg ggcaggaacc ggtcgctgat gtagcggtca gcgaggttgg cggcggcgac
     5761 caacgcgtcg tcggtgaagt tgacgcggtg gtgggcctcg tagcggtcgc gcaagccctt
     5821 gaggatctcg atcgtgtggg cgacggacgg ctcctgcacc tcgatgggct ggaaccggcg
     5881 ttcgagcgcc gcgtccttct cgaggtgttt gcggtactcg tctcgcgtgg tggcgccgat
     5941 ggtctgcagc tcaccgcgag cgagcatggg cttgaggatg ctggcggcgt cgatggcgcc
     6001 ttcggcggca ccggcgccga cgagcgtgtg cagctcgtcg atgaacagca cgatgtcgcc
     6061 gcgggtcttg atctccttca gcaccttctt gaggcgctcc tcgaagtcac cgcggtagcg
     6121 ggaaccggcg acgagggcgc cgaggtcgag cgtgtagatc tgcttgccct tcagcgtctc
     6181 cgggacctcg cccctgacga tgtcctggga caacccttcg acgacggcgg tcttgccgac
     6241 accgggctca ccgatcagca ccgggttgtt cttggtgcgc cgggacagga cctgcatgac
     6301 ccgctcgatc tccttctcgc gaccgatgac gggatcgagc ttgccgtctc gcgcgagctg
     6361 ggtgaggttg cgaccgaact ggtcgagcac catggaaccc tggccgctct gcccctcgcc
     6421 ctggccaccg ggggtggtct cgcccttgcc accggtgtag cccgacagca gctggatgac
     6481 ggtctgacgg acgcggttga ggtcggcgcc cagcttctgt agcacctggg ccgcgacgcc
     6541 ctcgccttcg cggatcagcc cgagcaggat gtgctcggtg ccgatgtagt tgtgaccgag
     6601 ctgcagcgct tcgcgcagcg acagctccaa gaccttcttc gcacgcgggg tgaaggggat
     6661 gtggccggtg ggcgcagcct ggccctggcc gatgatctcc tcgacctgct ggcggacggc
     6721 ttcgagcgag atgttcagcg attcgagggc cttggcggcg acgccttcgc cctcgtggat
     6781 gagaccgagc aggatgtgct cggttccgat gtagttgtgg ttgagcatcc tggcttcttc
     6841 ttgcgccagg acgacgaccc ttctggctcg atccgtgaac cgttcgaaca tgtcagcgtc
     6901 ctccgggccg cgaagcccac atgttccggc ctaggggatc tgcggaccac gtcctacccc
     6961 cgaggcacct gaggcgccgc acccggtgtc cggccgcggc gcccactgga gggaagtata
     7021 cccgtggggc ctgataaccc ttgaacgccc gctccatact tcccgtcctt ccgtcaccga
     7081 cccggtgatg ccctctgcag ggttattcgg acgttcaccg tcggaggatg agcatttccc
     7141 ccaagtcttc cgacaagtct gtcggcaggt tcttcggcaa cgtcgtgatc ccacgggatg
     7201 gcgcggccga tccctagggg tcgaacgccc caccgcgcct ggagggggcc tttcggccga
     7261 tgtcgcccat cccccatccg ggccatcacg atctcccccg tatgaccagt tgtgtcggcc
     7321 cgttgagcga tgcgaggatg tccgagcttt ccttgcgccg gcggcacgat cgaggggatc
     7381 cgcgtgcaac acgcctccga actgtacgaa ggagacatga gcttgcgagt cctcgtcgtg
     7441 gacgatcatc cggccttccg caaggccctc acgtcggcgc tccgcatggt ggccgacatc
     7501 gaggtcgcgg gcgaggccgg gggcgggatc gctgcctgcg aagaggccga gacgctcgag
     7561 cccgacatcg tgctgatgga cctgtccatg cccgacctgt ccggcatcga cgccatgaag
     7621 aagatccacg agtcccggcc cggcttgccc gtggtgatcc tgacggcgca cgccgacgaa
     7681 ggcgtcgagc gcgaggcgcg tgaggccggc gccaggggat tcctcgccaa gggcacgggc
     7741 ctgcaggacc tcgtgatcac gctgcacgag gccgtgggcg aggacgaacc ggtcctgtag
     7801 ccggcgtctc ggtccgcgta cccgctattc ctcgggtcgc tggctgggga acatgatcag
     7861 gtcgcgcagg ctcggtgcgt cggtcagcag catcagcgtc cgatcgatcc ccatgccgag
     7921 gccacctgcc gggggcatgc catgctccaa tgccctcagg aactcctcgt cgagcggatg
     7981 cgcttcgtcg tcgccagccg ccttcgcggc ggcctgcagc tcgaacttct cgcgttgttc
     8041 gatcgggtcg gtgagctcgc tgaacgcggt caccagttcg agaccgccga cgacgaggtc
     8101 gaagtgctcg gtgaggcggg ggtcgtcgcg gtgcggacgc gccagcggtg agacctcgcg
     8161 cgggaaatcg cagacgaacg tgggctgctc gatcgtgtct tccaccagct tctcgaacag
     8221 ctcctgcacg atcttgcccg gaccccatcc gtcctcgaga tggacctcgt ggcggcgggc
     8281 gatgtcggcg aggtcgggac gatcgagcgt gatctcctca ccggtggcct cgctcaccga
     8341 ccccaggatc gtgatgcgcc ggaacgccgg ttcgaggtcg atcgtgagat cccggtagcc
     8401 gatccgcagg tcgtcctcgg ggcgtcccac gatcgggttc acggcgcgag cgctctcgcg
     8461 aaggagcgtc tcggagagct ccatcatcga gcggtagtcc ccgtaggcct ggtaggcctc
     8521 gagcatcgtg aactccgggt tgtgatcgcg gtcgatgcct tcgttgcgga agttccgacc
     8581 cagttcgtag acgcgttcga cgccgcccac cagcatccgc ttgaggtaga gctcgagcga
     8641 gatgcgcagc ttcatctcct ggtcgagcac gtgatggaag gtggtgaacg gccgggcgtt
     8701 cgcgccgccc gccacgagct gcagcatcgg gccttcgaac tcgacgaacc cgcgctcgtc
     8761 caacacacgg cgcatcgtct gcaagacggc ggcacgcgcc aggaagtagc ggcgcggcac
     8821 gtcgtcggtg gccaggtgca ggtaccggcg acgctgctgc aggtcggggt ccttcaggcc
     8881 atgccatttc tcggggagcg gacgcaggct cttcgtgagc atcgcccatc gctccacgaa
     8941 gatcgacagc tcgcccttct tcgtcatccc caccgggccg ctggcggcga cgatgtcgcc
     9001 gaggtcggcc tcgtcgagca gggcgaacgc caccgggtcc atggccttgg cgttgcagaa
     9061 gagttggatg tcaccggtac ggtcgcggac caccaggaac ttcagtttgc ccatgtcgcg
     9121 ccgttgcacg atgcgtccgg cgaccgtccg tatgccatcg cccgtggtgc cggccgcgag
     9181 gtcgccgaac tcgcggtgga tgccagcgat ggcgtcgggt tcagcgaccc cgagcgcggt
     9241 ctccagcgtg agggcgaacg ggtccatgcc ggcgtcggtg agacgggcca ggctctcgcg
     9301 gcgcgcagcg aggatctccg cttcccgtga ggacccttgc tcgtcctcgg cggtgggttc
     9361 gtcctcctcg atgggttcgg cggactcgtc gtcgtgctca ctcatcggcg gtcaccacgt
     9421 tcttctcgaa gaccagccga aggccttcga gggtgagcga gggttcgtga tgatcgatcg
     9481 tggtgcagtg cgggatcacc gtgctcgcta gtccgccggt ggcgatcgtg gtggcgcccg
     9541 gcagctcggc acggatccgc gtcacgatcg cgtccacctc gccggcggtg ccgaagatca
     9601 gccccgattg gatcgcctcg accgtgctct tgccgaccac gctgggcggc gccgtgagtt
     9661 cgacccgcgt gagccgggcg gtgcgggaga acaagctcgc cgcgctcgcc tgcatcccgg
     9721 gcgcgatcac gccgccgatg aactccccct tggcgctcac cacgtcgaag ttcgtcgccg
     9781 tgccgaagtc gacgacgatg gccgggccgc cgaagcgcgt gaacgccgcg agggcgttga
     9841 ccacccggtc ggcacccacc tcgcgcggat tgtcggtgag gatcggcacc ccggtcttca
     9901 cgccgggtcc cacgatcacc ggtgggaacg ggaagtagcg cgccgccatc tcgcggacgg
     9961 cggcggtgac gtcgggcacg acgctggcca tcacgacgcc ggtgacctgg gactcgaacg
    10021 acatcccccg ctgcgccagg aagccgctca acgcgagcgc gagctcgtcg ggcgtgcgtt
    10081 cgggctgggt ggacaggcgc caggtgtgct cgagctcggg gccgcggaag accccgagca
    10141 cgatctcggt gttgccgacg tcggcggcga gcagcacggc ccgggtcccc tcccttcaga
    10201 tctccaacgc gaacgacggt ccatcgtggc ctacagggtc ctgccccgac aaccgcaagg
    10261 cgagggcgta gtcgccgaac gggtctcccg gctcgggttc gcggcgtccc gacgccacct
    10321 cggacaacga ggtgaggaac cgcagagcgg tgggcgcggc gccgcgcatc gccgccaccg
    10381 gaccgccgag ttcggcgacc tggtccccgt ccacgatcgg cgtgccttcg gacaccgacg
    10441 cccggcatcg cacccccatg cgaccgaatg cttctttgga gaccggcatg ccggccagga
    10501 tgccg
//
LOCUS       DATDBZ010000578         3802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2433562, whole genome shotgun sequence.
ACCESSION   DATDBZ010000578 DATDBZ010000000
VERSION     DATDBZ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433562"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..2473)
                     /locus_tag="VIX62_13350"
     CDS             complement(29..2473)
                     /locus_tag="VIX62_13350"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5861262.1"
                     /translation="MNAGTTMRARSDVRSRMASLIAVTVLVGVAGAVSIAAFTGAERA
                     STAYERFRVHTNEPEVIMFGGGNCPFSKNLDLKPMASLPPVESMQAGWNTFVNVYDQQ
                     GHLLIYSDPNFDGTLNAFDLDEPDSMRPLLLSGRYPRTADEVLMGWGESEVRRPVDGE
                     TIDVSIPRADAFGKIAGGDEPTEDDLLTVPLRVVGTAIMPGELDGTGGSLGVIPAFAD
                     RYRDEALGCDAGMFQLSGGPDGTAAFWTGSKQIAPGAFGFDTSTERTIAERATSLQSV
                     MLRLFGGLAALAAFLVLGQVLVRRTLLATTDAPVLRALGMSRGQLVRAALLPAVAVAL
                     LGTAIAIAGAVALSILTPLGDARIFEASPGVFIDPGILVVGALAIVVAVFACVGIPAW
                     RTSGDTGSMLGTTEFSGSDRHSRTAAAAATLGLPVSAVAGTRLALEPGHGRSATPVRS
                     AIVGLTLAVTAMVAAFGFAASMDRFTSSPTLWGIRFDFATGHPFIGDVFQKEAIPIVL
                     DDPDVGDLTVGNFQEQIGLVGPNGTQSVAIWGMESLKGDPIDLTMLEGRWPDADDEIA
                     VGLQTARALGVSVGDHVEAELRSTTKDLTVVGIPVFPDFGFGPGLGQGVGSTMDLLHE
                     FYPEATQNLLLARFAPAVDREVSLERINRALLEVNRDVAVETPESGELGSSLQHTRKS
                     RELPLILAGLFGLVALATLVHVLVTSVRRRRRDLAILRTIGFTRRQIVATIAWQATTI
                     AVIALLIGVPLGFLVGRFTWALFADHLGVVSLPVEAWGSVAIVVPTVLILANLVAIGP
                     AMFARRTQPAQVLRAE"
     gene            complement(2470..3225)
                     /locus_tag="VIX62_13355"
     CDS             complement(2470..3225)
                     /locus_tag="VIX62_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5861263.1"
                     /translation="METDAKALVTVGLRKTFESTDDDQIPVRALRGLDVSVDRGEFVA
                     VMGPSGCGKSTMLNLIAGLDTPTDGTIEIDGEPITGLSEDDLAMMRRRHIGIVFQFFN
                     LLEGMTVLENVAMPAAVAGRSRRQAETRGRDLLDLLGIADKASVAPGRLSGGQRQRLS
                     IARALANEPTLLLADEPTGSLDSAGGAEVLELFRRLHEGGQTIVMVTHDDDVASVSDR
                     IVRMRDGRIDDGREHAATSAAGGVFAAWEEPAT"
     gene            complement(3315..>3802)
                     /locus_tag="VIX62_13360"
     CDS             complement(3315..>3802)
                     /locus_tag="VIX62_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861264.1"
                     /translation="FREAARDAIELAQGPPGGLAAATVVIDAAAEYKKNLKGELDEEL
                     APFMDEKGRPEDAYRGAIKRIETRFKRKERRAERDYVDWVLLAVSALLRDRIAAGVGV
                     RAADLLNPDLAPGDVLGVTRAAKGLAGIEAARAELADDFNLNVRLVLERAFLSLVELA
                     A"
BASE COUNT          632 a         1352 c         1274 g          544 t
ORIGIN      
        1 gagccacttg atccacgcct tcatgccgtc actccgcacg gagcacctgg gccggttgcg
       61 tccggcgcgc gaacatcgcc ggtccgatcg ccaccaggtt cgccaggatc aggacggtgg
      121 gcaccacgat ggcgacgctc ccccacgcct cgaccggcaa cgacacgacc ccgagatggt
      181 cggcgaacag ggcccaggtg aagcggccca ccaggaaccc cagcggcacg ccgatcagga
      241 gcgcgatcac ggcgatcgtc gtggcctgcc aggcgatcgt ggcgacgatc tgccgccggg
      301 tgaacccgat cgtgcgcagg atcgcgaggt cccgccggcg ccgtcgtacg gaggtcacga
      361 gcacgtggac gagggtcgcc agcgcgacca gaccgaacag cccggccaag atcaggggca
      421 gttcccgcga cttccgcgtg tgctgcagcg acgagccgag ttcgcccgac tccggcgtct
      481 ccacggcgac atctcggttc acctcgagga gcgctcggtt gatccgctcg agggatactt
      541 cacggtccac agccggggcg aagcgcgcga gcaggaggtt ctgggtcgct tcgggataga
      601 actcgtgcag cagatccatc gtggagccca cgccctgacc caaacccggt ccgaagccga
      661 aatccgggaa caccgggatg ccgacgacgg tgaggtcctt ggtcgtgctt cggagctcag
      721 cctccacgtg gtcgccgacc gacaccccga gggcccgcgc ggtctgcagg ccgaccgcga
      781 tctcgtcgtc ggcatccggc caccgccctt cgagcatcgt gaggtcgatc gggtctccct
      841 tcagcgattc catcccccag atcgccaccg attgggtacc gttcggcccc accaagccga
      901 tctgctcctg gaagttcccc accgtcagat cgcccacatc ggggtcgtcg agcacgatcg
      961 ggatcgcctc cttctggaac acgtcaccga tgaacggatg accggtggcg aagtcgaacc
     1021 ggatccccca caaggtgggg gacgacgtga accgatccat gctcgcggcg aagccgaagg
     1081 ccgccaccat cgcggtcacc gcgagggtga ggccgacgat cgcgctgcgg acgggcgtcg
     1141 cgctccgtcc gtgcccgggc tccagcgcca gcctcgtccc ggccacggcc gaaacgggca
     1201 ggcccaacgt cgccgccgcg gcggcagtcc gagaatggcg gtcactcccg gagaactcgg
     1261 tggtgcccag catcgagccc gtgtccccgg aggttctcca cgccgggatc ccgacacagg
     1321 cgaacaccgc cacaacgatg gccagggcgc ccacgacgag gatgccgggg tcgatgaaca
     1381 ctccgggcga cgcctcgaag atccgggcgt cccccaacgg ggtgaggatc gacaaggcca
     1441 cggcacccgc gatcgcgatc gccgttccga gcagcgccac ggcgaccgcc gggagcaacg
     1501 cggcccggac cagctgccca cggctcatcc cgagggcacg cagcaccggg gcgtccgtcg
     1561 tcgccagcaa cgtccgacga acgaggacct gccccaagac caagaaggcc gcgagcgccg
     1621 cgagcccgcc gaacaggcgc agcatcaccg actggaggga ggtggcacgc tcggcgatcg
     1681 tccgctccgt cgaggtgtcg aacccgaacg cccccggagc gatctgcttg gatccggtcc
     1741 agaacgccgc cgtgccgtcc ggtccacccg acaactggaa catgcccgcg tcgcacccga
     1801 gcgcctcatc ccggtaccgg tcggcgaacg ccgggatcac gcccaaggaa ccaccggtgc
     1861 cgtccagctc gccgggcatg atcgcggtcc ccaccaccct caggggaacg gtgagcaggt
     1921 cgtcctccgt cggctcatcg ccaccggcga tcttcccgaa cgcgtcggcc ctcgggatcg
     1981 acacgtcgat cgtctctccg tccacggggc gccgcacttc tgactcgccc caccccatca
     2041 gcacctcatc ggcggtacgc gggtaccgcc ccgagagcaa caggggacgc atcgaatccg
     2101 gttcgtcgag gtcgaaggcg ttcagggtgc cgtcgaagtt gggatccgag tagatcaaca
     2161 gatggccttg ctggtcgtac acattgacga acgtgttcca cccggcttgc atcgactcca
     2221 ccggcgggag cgaagccatc ggcttgagat caaggttctt cgagaagggg cagttcccgc
     2281 cgccgaacat gatcacttcg ggctcgttcg tatgcacccg gaagcgctcg taggcggtac
     2341 tcgcccgctc cgcgcccgtg aacgcagcga tggacaccgc gcccgccacc ccgaccagca
     2401 cggtgacagc gatgagggat gccatacgcg atcgcacgtc ggagcgtgcg cgcatcgtgg
     2461 tgccggcgtt cacgtggccg gttcctccca cgcggcgaac acgccgccgg cagcgctcgt
     2521 ggcggcgtgc tcccggccgt cgtcgatgcg gccgtcccgc atgcgcacaa tgcggtcgga
     2581 cacggaggcc acgtcgtcgt cgtgggtcac catgacgatc gtctggcccc cttcgtggag
     2641 acggcggaac agctcgagca cctcggcgcc cccggccgag tcgagcgagc cggtgggctc
     2701 gtcggcgagc agcaaggtgg gctcgttcgc gagcgcacgg gcgatcgaca gccgctgacg
     2761 ctgcccgccg gacaggcggc caggtgcaac cgaggccttg tccgcgatgc ccagcaggtc
     2821 gagcagatcc cgtccccgtg tttccgcctg tctgcgtgag cgccccgcaa ccgcggccgg
     2881 catggcgacg ttctcgagca ccgtcatgcc ttcgaggagg ttgaagaact ggaacacgat
     2941 cccgatgtga cgacggcgca tcatggccag atcgtcttcg gacaggcccg tgatcggttc
     3001 gccgtcgatc tcgatcgtgc cgtcggtcgg cgtgtcgagg ccggcgatga ggttgagcat
     3061 ggtcgacttg ccgcatcccg aggggcccat gacggccacg aactcaccgc gatcgaccga
     3121 gacgtccagc cccctgaggg ctcggacggg gatctggtcg tcatcggtgg actcgaacgt
     3181 cttgcgcaat ccgaccgtga cgagcgcctt ggcatcggtt tccatggcgg gagggtacgc
     3241 cgtggcccgg cgccgaggcc acgaggaggc caccaccttc tccggtgaag gtggcacccc
     3301 gcgttgcgtg ctcctcaggc ggcgagttcg accaacgaca ggaaggcccg ctccagcacg
     3361 agccgcacgt tgaggttgaa atcgtcggcg agttcggctc gggcggcctc gatcccggcg
     3421 aggcccttgg ccgcccgggt gactcccagg acgtcgccag gagcgaggtc ggggttcagc
     3481 aggtcggcgg cgcggacacc gacgccggcg gcgatgcggt cgcggagcag cgcggagacg
     3541 gccagcaaca cccagtcgac gtagtcgcgt tcggcgcggc gttccttccg cttgaaccgg
     3601 gtctcgatcc gtttgatcgc accgcggtag gcgtcctcgg gacggccctt ctcgtccatg
     3661 aacggcgcga gctcctcgtc gagctcgccc ttgaggttct tcttgtattc agcggcggcg
     3721 tcgatcacga cggtggcggc ggcgagtccg ccgggagggc cctgcgcgag ctcgatcgcg
     3781 tcgcgcgcgg cttcgcggaa ct
//
LOCUS       DATDBZ010000579         3346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_596338, whole genome shotgun sequence.
ACCESSION   DATDBZ010000579 DATDBZ010000000
VERSION     DATDBZ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596338"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VIX62_13365"
     CDS             complement(<1..375)
                     /locus_tag="VIX62_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861265.1"
                     /translation="MTQPEPPAPARWQEVPPAPSLGNDLRRAAPVALRRGLTVSFVAL
                     LLGQLPPLAFNLGGAGLAIATQLRLGWLYTVAANAAAIRIDEFSTATGDPVLYGVANF
                     RLALLTLTAVIVWMLVRAGAASG"
     gene            complement(372..1241)
                     /gene="sucD"
                     /locus_tag="VIX62_13370"
     CDS             complement(372..1241)
                     /gene="sucD"
                     /locus_tag="VIX62_13370"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY5861266.1"
                     /translation="MSVLVGADTKLLVQGITGNEGTFHTLRNRAYGTQVVAGVTPGKA
                     GQDVEGIPVFDTVAEAVEETGANTAMIFVPPRFAGEAILESADADVPLVICITEGIPV
                     KDMAYAHSYLRGRSTTLVGPNCPGVITPGVANVGIIPGEICAPGRVGLVSRSGTLVYQ
                     IVHELTQRGVGQSTCIGMGGDPVHGVGFIESLALFEADDATDLVVMTGEIGGDDEERA
                     AAYIAEHVTKPVVGYIAGFEAPPGKRMGHAGAIVTGSQGTAAAKAEALEAVGVRVART
                     PTQVAELVAEGLT"
     gene            complement(1238..2383)
                     /gene="sucC"
                     /locus_tag="VIX62_13375"
     CDS             complement(1238..2383)
                     /gene="sucC"
                     /locus_tag="VIX62_13375"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY5861267.1"
                     /translation="MDLYEYQGKELFRAQGIVTPTGIVARSGDQAAEATRELGGKSVV
                     KIQVQVGGRGKGGGVVVVDSPERAAAEATRMLGSDFKGHEVTQVLCEELLPIAQEFYT
                     SILLDRSTGDHLAMMTAEGGMDIEELARTRPEALRRVHIDPMLGLRAFHVRELVGTLP
                     QEAREGAADVLRRLYAMLQERDATLVEVNPLVLLEDGRVVALDAKVTVDDNSLFRHED
                     IEAMKAAFPIDPVQARANEQDLQYVKLDGNVGIIGNGAGLVMSTLDVVAQAGAKAANF
                     LDVGGGASADQMATSLEVVLSDPAVKAVFINIFGGITRCDLVAQGVLGALERVEATVP
                     IVVRLDGTNAEEGREILAKAAHPRIVAASTMLEAAERAAGFANGEAA"
     gene            complement(2408..2824)
                     /locus_tag="VIX62_13380"
     CDS             complement(2408..2824)
                     /locus_tag="VIX62_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY5861268.1"
                     /translation="MAGKHRVIVAKPGLDGHDRGAKVVARALRDAGFEVIYTGLHQTP
                     EQIAETALQEDADAVGLSCHSGAHMTLFPKVVQELRARGADDILVFAGGIIPVDDIAA
                     LKAAGIEEIFTPGANTGDIAAWLHQRLGDETSATSP"
     gene            2989..>3346
                     /locus_tag="VIX62_13385"
     CDS             2989..>3346
                     /locus_tag="VIX62_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5861269.1"
                     /translation="MERPETRYAKTADGVHVAYQVFGGGPIDILFVLGWVTHIERMWD
                     QPRVARFLTRLASFSRVMLFDKRGVGLSDRVPEDRLPSLEVRMDDARAVMDAAGSERV
                     IVLGHSEGGPMATLFAA"
BASE COUNT          516 a         1173 c         1099 g          558 t
ORIGIN      
        1 ccccgacgcc gccccggccc gaacgagcat ccagacgatg accgcggtca acgtgagcag
       61 agccaaccgg aagttggcga ccccgtacag caccggatcc ccggtcgccg tcgagaactc
      121 atcgatccgg atggcagcgg cgttggcggc cacggtgtag agccatccaa ggcgcaactg
      181 ggtagcgatc gccagccctg ccccgccgag gttgaacgcc agcggtggca gttggccgag
      241 caggagggcg acgaacgaca ccgtgagccc gcgcctcagc gcgaccggcg ccgcgcgacg
      301 gaggtcgttg ccgagcgaag gtgccggcgg aacctcctgc caccgcgcgg gcgcgggagg
      361 ttccggctgc gtcacgtcag accctcagcc accagttcgg cgacctgcgt cggcgtccgc
      421 gcgacccgga ccccgacggc ttccaacgcc tcggccttcg ccgccgccgt cccttgggac
      481 cccgtgacga tcgcgccggc gtgacccatc ctcttgccgg gcggcgcctc gaacccggcg
      541 atgtaaccca cgaccggctt cgtgacgtgc tcggcgatgt aggcggcagc ccgttcttcg
      601 tcgtcgccac cgatctcccc ggtcatcacg accaggtcgg tggcatcgtc ggcttcgaac
      661 agcgcgagcg actcgatgaa cccgacgccg tgcaccgggt cgccacccat gccgatgcag
      721 gtggactgcc cgacacctcg ctgagtgagc tcgtgcacga tctgatagac gagcgtgccg
      781 cttcgactca ccaaaccgac gcgacccggc gcgcagatct cgccggggat gatgcccacg
      841 ttggcgacac cgggcgtgat cacgccggga cagttgggac ctaccagagt ggtcgagcgg
      901 ccccgcaggt agctgtgcgc gtaggccatg tccttcaccg ggatgccttc ggtgatgcag
      961 atcaccagcg gcacgtcggc gtcggccgat tccaggatcg cctccccggc gaaccgcggc
     1021 ggcacgaaga tcatggcggt gttcgcgccg gtctcctcga ccgcctcggc gacggtgtcg
     1081 aacaccggga tgccctcgac atcctggccc gccttgccgg gcgtgacgcc cgccaccacc
     1141 tgggtgccgt aggcacggtt gcgcaaggtg tggaacgtgc cctcgttgcc agtgatgccc
     1201 tgcacgagga gtttggtatc ggcgccgacg aggacgctca tgccgcctcc ccgttggcga
     1261 aaccggcggc tcgctcggcc gcttcgagca tcgtggacgc agcgacgatc cgcgggtggg
     1321 cggccttcgc caggatctcg cggccttctt cggcgttggt gccgtccagt cgaacgacga
     1381 tcggcacggt ggcctccact cgctccagcg cgcccaacac accctgggcg accaggtcgc
     1441 agcgcgtgat gcctccgaag atgttgatga acacggcctt gaccgcgggg tcggacagca
     1501 cgacctcgag cgaggtcgcc atctgatcgg cactggcccc cccaccgacg tccaggaagt
     1561 tggccgcctt cgcaccggct tgggccacca cgtcgagcgt ggacatcacc aaacccgcgc
     1621 cgttaccgat gatcccgacg ttgccgtcca gcttcacgta ctgcaggtct tgttcgttcg
     1681 ctcgcgcctg caccgggtcg atcgggaagg cagccttcat ggcctcgatg tcctcatgtc
     1741 ggaacagcga gttgtcatcg acggtgacct tggcatcgag ggcgacgacc cggccgtctt
     1801 cgagcaacac cagcgggttc acctccacca gggtcgcatc gcgttcctgc agcatcgcgt
     1861 agagccggcg gagcacatcg gcggcgcctt cgcgcgcctc ctgcggcagg gtgccgacca
     1921 gctcacggac gtggaacgct cgcaggccga gcatcggatc gatgtggacc cgccggagcg
     1981 cttcgggacg cgtgcgggcg agttcttcga tgtccatgcc gccctcggcg gtcatcatcg
     2041 cgaggtggtc ccccgtggat cggtcgagca ggatcgaggt gtagaactcc tgggcgatcg
     2101 gcaggagctc ttcgcacaac acctgcgtga cctcatgacc cttgaagtcg gatccgagca
     2161 tgcgcgtggc ctcggccgcg gcgcgctcgg gcgaatcgac gacgaccacg ccgccccctt
     2221 tgccacggcc ccccacctgt acctggatct tgacgacgct cttgccaccc agctcgcgtg
     2281 tggcttcggc ggcttggtct ccggagcgag cgacgatccc ggtgggtgtc acgatccctt
     2341 gcgcgcggaa cagctcctta ccttggtact cgtacagatc catggaactc cctgggtcag
     2401 tagtctgcta tggactagta gcactcgtct cgtccccgag ccgctgatgc agccacgccg
     2461 cgatgtcgcc cgtgttggcg cccggcgtga agatctcttc gatgcccgct gccttcagcg
     2521 ccgcgatgtc gtcgaccggg atgatgcccc ccgcgaagac caggatgtcg tcggcacctc
     2581 gtgcgcgcag ttcttgcacc actttgggga acagtgtcat atgcgccccg gagtggcagg
     2641 acaaccccac cgcatcggcg tcttcttgca gcgcggtctc ggcgatctgt tccggggtct
     2701 gatgcaggcc ggtgtagatc acctcgaacc ccgcgtcacg cagggcgcgg gccaccacct
     2761 tggccccacg gtcgtgcccg tcgagaccag gtttcgccac gatcacgcgg tgcttgccgg
     2821 ccatgtgcgc ggcagtctaa cgaacgggtc gcgcggaccc cggttgcggc aaaggccgcc
     2881 tgcgacgcaa gatgcccctc gtggagtcgc ctctcgcaga acgcttcgtc tgctcagcag
     2941 agcagggcgg gccgggccgt tggcacctct gtcaggtggt gagctgagat ggagcgtcca
     3001 gagacccgtt acgccaagac cgcggatggt gtccatgtcg cctaccaggt gttcggcggg
     3061 ggcccgatcg acatcctgtt cgtcctcggg tgggtcaccc acatcgaaag gatgtgggat
     3121 cagccgaggg tcgcccgttt cctgacgcga ctcgcgtcgt tctcgcgcgt gatgctgttc
     3181 gacaagcgag gcgtcgggct ttcggaccgc gttccagagg atcggctgcc atcgctcgag
     3241 gtgcggatgg acgacgcccg cgccgtcatg gacgcggccg gctcagagcg cgtgatcgta
     3301 ctcggccact ccgagggtgg gccgatggcc accctgttcg ccgcca
//
LOCUS       DATDBZ010000580         2148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2847328, whole genome shotgun sequence.
ACCESSION   DATDBZ010000580 DATDBZ010000000
VERSION     DATDBZ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2847328"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VIX62_13390"
     CDS             <1..676
                     /locus_tag="VIX62_13390"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HEY5861270.1"
                     /translation="AYVRAHDFSVTAAQRMCQSQLRAIMSADDQFAATMQLLSDRGVL
                     SDTLVIFSSDNGFMWGEHGRTEKFVPYEPSIRVPLLVRWPGHFTAGVDSTRIASYLDI
                     LPTMLEAAGVTLPTDAPRMDGESLLQPSSRTSAFAEYYQDATNGDIPTWKMVRTPTAK
                     YVQIYDSNGAVVFREYYNLVSDPAENTNLLGDANTANDPSASTITSMRNLLNIYATCV
                     GTVCVR"
     gene            complement(684..1391)
                     /locus_tag="VIX62_13395"
     CDS             complement(684..1391)
                     /locus_tag="VIX62_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861271.1"
                     /translation="MSVQASDRRTAGALAIEPVVGWRVWRLQRVDSRLTLVSATRDNA
                     WPAADAIEARCWIDHGTAGVPQAACRCGIYATSSPSALPQANVISPETCVVGAIAMWG
                     SVVEHVRGARSQFAYPARLRLVCGRCLATGMGAIEPARAREWEGIITAVCDRHAGGFL
                     TTRAATEVQQELLSTYAVDLLPIQRLQRGLIPVRDTRQQVRGAVRAVAAALFGGMNAW
                     STAWPVRPKGRDRRTGV"
     gene            1532..1747
                     /locus_tag="VIX62_13400"
     CDS             1532..1747
                     /locus_tag="VIX62_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861272.1"
                     /translation="MPASPPCEYVDVALHFQQMIRDRHPSSGDASATVAIGEPCGEPS
                     IGAMRVDDGTVGHFCDLHLRQPKLVRG"
     gene            1804..>2148
                     /locus_tag="VIX62_13405"
     CDS             1804..>2148
                     /locus_tag="VIX62_13405"
                     /inference="COORDINATES: protein motif:HMM:NF042641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEY5861273.1"
                     /translation="MARLSAKERAGLPDRAFAYIDARGNRRLPINDEAHVRAALGRFE
                     RVRFDDDAERERARKRLLNAAKKYGIVPVGFITGQLRSERTARTPDFSTFPTGAVTFL
                     LTDIEGSTALLRT"
BASE COUNT          359 a          769 c          677 g          343 t
ORIGIN      
        1 tgcctacgtg cgggcccacg acttcagcgt cacagccgcc cagcggatgt gccagagcca
       61 gctccgcgcg atcatgagcg ccgatgatca gttcgccgcg acgatgcagc tgctgtccga
      121 tcgcggggtt ctgagcgata ccctcgtgat cttctcgtcg gacaacggct tcatgtgggg
      181 cgagcacggt cgcacggaga agttcgttcc gtacgagcca tcgatacgcg tccccttgtt
      241 ggttcgctgg cccggccact tcactgcggg tgtcgactcg acccgtatcg cctcatacct
      301 cgatatcctc ccgacgatgt tggaggcggc gggcgtcacg ctccccaccg acgcccctcg
      361 catggacggc gaatccctgc tgcaaccgtc gagccgcact tcggcgttcg ccgagtacta
      421 ccaggacgcg acgaatgggg acatccccac gtggaagatg gttcgaaccc ccaccgcgaa
      481 gtacgtccag atctacgact ccaacggagc ggtggtcttc cgggagtact acaacttggt
      541 gagcgatccg gcggagaaca cgaacctgct gggcgatgcg aacacggcga acgacccgtc
      601 ggcgagcacg atcacctcca tgaggaacct gttgaacatc tacgccacgt gtgtcggcac
      661 cgtctgcgtc aggtagcggt cactcaaaca cccgttcggc gatcgcgccc cttcgggcgg
      721 accggccacg ccgtagacca ggcgttcatt cctccgaata gggcggcggc cacggccctc
      781 accgcgcctc gcacctgctg ccgggtgtca cgaacaggga tcaacccacg ctggaggcgc
      841 tggatcggga gcaggtcgac cgcgtacgtc gacaggagct cctgctggac ctcggtggcg
      901 gcgcgcgtgg tgaggaaccc accggcatgt cggtcacaca ccgcggtgat gatcccttcc
      961 cactcgcgcg ctcgcgccgg ttcgatcgca cccatgcccg tggcgaggca ccgaccgcac
     1021 accaggcgca ggcgcgccgg gtacgcgaac tgcgaccgcg ctccgcgcac gtgctcgacc
     1081 accgaccccc acatggcgat ggcgcccacg acgcaggttt cggggctgat gacgttggcc
     1141 tgcggcagag cggacggcga cgacgtggca tagatgccgc accggcatgc ggcctgcggc
     1201 accccggccg tcccgtgatc gatccagcac ctcgcctcga tcgcgtcggc tgccggccag
     1261 gcgttgtccc tcgtcgccga gaccaacgtc agtcggctat cgacacgctg cagccgccac
     1321 acgcgccagc caacgacggg ttcgatcgcg agcgcgcccg cggtgcgacg gtccgatgcc
     1381 tgaacggaca tgatggaccc gatgctactc ctggccgccg gcgaacgggg agccaaccag
     1441 catccggcgg tccatggctg agaccttcgg accaaagaag tcggtcggat agccgatgcg
     1501 aaggctcggg ggtttggccg acactggctc catgcctgcc tccccaccgt gcgaatacgt
     1561 cgacgttgcc ctgcacttcc aacagatgat ccgcgatcgc cacccttcct ctggcgacgc
     1621 cagcgccacc gtcgcgatcg gcgagccctg cggcgaaccc tcgatcggtg ccatgcgcgt
     1681 cgacgacggc accgtcggcc atttctgcga tctgcacctg cgccagccca agctcgttcg
     1741 cggctgacgc aggcccgcct ttcccggtcg tgggcgtcgt cgcctccggg tatcgtcgga
     1801 ggcatggcac gactcagtgc gaaggaacgg gccgggctgc ccgatcgtgc gttcgcctac
     1861 atcgacgcgc gcggcaaccg taggctcccc atcaacgatg aagcgcatgt ccgggccgcg
     1921 ctcggccggt tcgagcgggt gcggttcgac gacgatgccg agcgcgaacg cgctcgtaag
     1981 cgcctgctca acgcagccaa gaagtacggc atcgtgccgg tggggttcat caccggtcag
     2041 ctccgttccg agcggaccgc acgcacgccg gacttctcca ccttcccgac cggcgcggtc
     2101 acgttcctgc tcaccgacat cgagggttcc accgcgttgt tgcgcacg
//
LOCUS       DATDBZ010000581         2517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1213068, whole genome shotgun sequence.
ACCESSION   DATDBZ010000581 DATDBZ010000000
VERSION     DATDBZ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213068"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VIX62_13410"
     CDS             <1..992
                     /locus_tag="VIX62_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY5861274.1"
                     /translation="VQISTRVQLALALISIAVVLVFFVTVIADLGSANDFAKSVDPSP
                     EGGFSGILFGVLYGVLIFVGFETAANLAEETAEPKRAIPRAVLGAVVLVSIFYVIAAY
                     VEVAGFGFDMAVITSPEVAGAPLFALGAPGGQFGSEFWLKLLMVVVFLDMLAVYVGAG
                     VASTRGVFAMARDRRLPAPLASVSSKFGTPSGAIFLLIAMQVLFIYLAQSTEVFGLEG
                     LPHYFALFAWASTFGGFALLVVYLMMSVGALITLGKQEKPVGVAVAAVVGIALTGAAI
                     WGSFYKVPSPTIWAPRYALIWLVIGVIYMLLVKGRERASQVLGDLSEPGGTAS"
     gene            1033..2463
                     /locus_tag="VIX62_13415"
     CDS             1033..2463
                     /locus_tag="VIX62_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5861275.1"
                     /translation="MTGEGRTLRLDAPRDEALQHAVRLVTEAWTSFDRFRPEEPPLDE
                     RVRRLLEASLPDASTPVHEALDDAAHILDESIAQARPRYFAFIGSSGLEIGALADFLA
                     HTYDINLAVDARAATQVEDQAVRWAAEFVGFPAAGGAFTSGGTISNVSALAAARERAL
                     PGSRHTGLAGRRMAVYCSNEVHYSITRAIELLGIGSDNLRAIPLDGLRRMQPDALARS
                     IDDDAARGVTPVAVVATAGTTLTGAIDPIDAIADVCAPRGVWLHVDGAYGLPAASTPS
                     RSADFAGLARADSCSIDAHKWLYLPKACGVVLVRDADALAGAFAHEQGYLPHQQHELH
                     AVDITLEYSRPFRALKLWLAFRAHGASQFREAIEANLAEADLLYRHAQASEDFEVLDA
                     PPQLSIVPLRHVPPGVDDLNAHNQALADAIQADGRVFLASALIDGEVWLRPCFVNFRT
                     TEEDVLALLEVARELGARLAGGGDEA"
BASE COUNT          354 a          808 c          885 g          470 t
ORIGIN      
        1 gggtccagat ctcgacacgc gttcagctgg cgctggcgtt gatctcgatc gcagtggtcc
       61 tggtgttctt cgtgacggtg atcgccgacc tcggcagtgc caacgacttc gcgaagtccg
      121 tcgaccccag ccccgaaggc ggcttctcgg ggatcctgtt cggcgtgctc tacggggtgt
      181 tgatcttcgt ggggttcgag accgccgcca acctggcgga ggagaccgct gagcccaaac
      241 gagcgatccc acgcgcggta ctgggtgcgg tcgtgctggt gtcgatcttc tatgtgatcg
      301 cggcctacgt cgaggtggcg gggttcgggt tcgacatggc agtcatcacc agccctgagg
      361 ttgccggcgc gccgctgttc gcgctcggtg ctccgggcgg ccaattcggg tctgagttct
      421 ggctcaagct cctcatggtg gtcgtcttcc tcgacatgct cgcggtctac gtgggcgccg
      481 gcgtcgcctc gacccgcggg gtcttcgcga tggcccgaga tcgacgcctc ccggcgccgc
      541 tagcatcggt gtcgagcaag ttcgggacac cgtcgggggc catcttcctg ttgatcgcga
      601 tgcaggtgct cttcatctat ctcgcccagt cgaccgaggt cttcgggttg gaaggtctgc
      661 cccactactt cgccctgttc gcgtgggcat cgacgttcgg tgggttcgcc ttgttggtgg
      721 tctacctcat gatgtcggtg ggggcgctca tcaccctcgg caaacaggag aagccggtag
      781 gggtggcggt cgccgcagtc gtgggcatcg cgctgacggg tgccgcgatc tgggggtcgt
      841 tctacaaggt gccgagcccc acgatctggg cacctcgata cgccttgatc tggctggtga
      901 tcggggtgat ctacatgctc ctcgtcaagg gtcgcgagcg cgcgagtcag gtgctgggcg
      961 atctgtcgga gccagggggt accgcgagct agctcgacgc ggcggccggt gtggcctcac
     1021 gagacaggag tcatgacggg cgaaggacgg acgctgcggc tggatgcgcc tcgcgatgag
     1081 gcgctccagc acgccgtccg gctggtcacc gaagcgtgga cgagtttcga tcggttccgg
     1141 cctgaggagc cgccccttga cgagcgggtc cggcgtctgc tcgaggcctc gttgccggat
     1201 gcatcgaccc cggtgcatga agcgctggat gatgccgcgc acatcctgga cgaatcgatc
     1261 gcgcaggcga ggccgcgcta cttcgcgttc atcggctcga gcgggttgga gatcggtgcg
     1321 ctggccgact tcctggcgca cacgtacgac atcaatctgg cggtcgacgc gcgggccgcg
     1381 acccaggtcg aagaccaagc ggtgcgatgg gccgctgagt tcgtgggttt ccccgccgcg
     1441 ggaggcgcgt tcaccagcgg tggcacgatc agcaacgtca gtgccctcgc cgcggcgagg
     1501 gagcgtgcgc tgccgggatc gcgtcacacc ggacttgccg gacgccgcat ggcggtctac
     1561 tgctccaacg aggtgcacta ctcgatcacg cgtgccatcg agctgctcgg gatcggctcg
     1621 gacaacctgc gggcgatccc gctcgatgga ctccgccgca tgcaacccga cgcgttggcc
     1681 cgatcgatcg acgacgacgc ggcacgcggc gtcacacccg tcgcggtggt ggcgaccgcc
     1741 ggcaccacgt tgacgggtgc gatcgatccg atcgatgcca tcgcggacgt gtgtgcgccg
     1801 cgaggtgtgt ggctccacgt cgacggggcc tacggccttc cggcagcgtc caccccgtcc
     1861 cgctcggctg atttcgcggg actcgcgcga gcggactcct gctcgatcga cgcgcacaag
     1921 tggttgtact tgccgaaggc gtgtggcgtg gtgctcgtgc gtgacgccga cgccttggcc
     1981 ggcgcgttcg ctcacgagca gggctacctg ccccaccagc agcatgagct gcatgcggtc
     2041 gacatcacgc tggaatactc ccggccattc cgcgcgctca agctgtggct cgcgttccgc
     2101 gcgcacggtg cgtcgcagtt cagggaggcg atcgaggcca acctcgccga ggccgacctg
     2161 ctctaccgcc acgcccaggc gagtgaagac ttcgaggtgc tcgatgcgcc accgcagctt
     2221 tcgatcgttc cgctgcggca cgtgccgccc ggggtcgatg atctcaacgc ccacaaccag
     2281 gccctcgcgg atgcgatcca ggccgacggg cgcgtgttcc tcgcgtcggc cttgatcgat
     2341 ggcgaggtgt ggcttcgccc gtgcttcgtc aacttccgca cgaccgagga ggacgtcttg
     2401 gccttgctcg aggtggcgag ggagttgggt gcgcgtctcg caggtggcgg ggacgaggca
     2461 tgagcgatca gcgcagcatg ggcgaccaac atcttccggg cgaggatggg ctcttgc
//
LOCUS       DATDBZ010000582         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_597989, whole genome shotgun sequence.
ACCESSION   DATDBZ010000582 DATDBZ010000000
VERSION     DATDBZ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597989"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1073)
                     /locus_tag="VIX62_13420"
     CDS             complement(96..1073)
                     /locus_tag="VIX62_13420"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEY5861276.1"
                     /translation="MADAEVGDAWYGDDPTANRLQELAAERTGTQAALFVPTGTMANQ
                     IALALHVRTNGHLVASSAAAHVATTELMTSAALSGIAFRTVDPGARGWIDASHARSLL
                     EPDSYYDVEIIDLLCVENTVGGAGGTVMPVDELAKVRTVASEVGVPMHMDGARVFNAA
                     AAAGVDVTEWTGQVDTLMFCLSKGLGAPIGSMICGPSDMIREARRLSILFGGAWRQAG
                     MMAAAGIIALEEGSQRLHEDHERAQVLATTVGEMLPNSIDPDQVATNMVFVDTEAVGM
                     PVLETVERLAALGVGVTHVDTHVRMVTHVDVDDDGVAFALEAWRSIAPA"
     gene            complement(1199..2227)
                     /locus_tag="VIX62_13425"
     CDS             complement(1199..2227)
                     /locus_tag="VIX62_13425"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY5861277.1"
                     /translation="MRLGILTGGGDCPGLNAVIRASVRKGVGVHGHSFVGFRDGWRGV
                     LEDESIDIGIPEVTGILHRGGTILGSSGVNPMRVEDGPAIVRATFERRGLDGLIAIGG
                     EGTLGAAAQLADEGLPVVGVPKTIDNDLGGTDITVGFHTAVQVATDSIDRLHSTAESH
                     DRVMLVEVMGRHAGWIAVYAGMAGGADVILIPEQPFEIDEVCHRLRMRHMQRSSFSIV
                     VVAEGAIPAEGTLVLPEPELDENGFPRLGGVSTHIAPEIEERTGFQTRVTILGHVQRG
                     GSPVAFDRVLATRMGLGAVALANAGGWGRMVARRAQEIIDVPLTSAVAERKVVPLELY
                     REAEVFFG"
     gene            2295..2474
                     /locus_tag="VIX62_13430"
     CDS             2295..2474
                     /locus_tag="VIX62_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861278.1"
                     /translation="MSFIATSRLASSFWPEMPGFLEKFFVGLLALLVVAVGVFALFLI
                     ANQFRNPGRRPGEGP"
     gene            complement(2484..>2748)
                     /locus_tag="VIX62_13435"
     CDS             complement(2484..>2748)
                     /locus_tag="VIX62_13435"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEY5861279.1"
                     /translation="ASTLLILTDVEKVQRGYGSFMPEDIDRLTVKEATALLKKGEFGA
                     GSMGPKVEAAATFARSGGRAVIAELADAPAALSGEAGTQVVKG"
BASE COUNT          440 a          985 c          865 g          458 t
ORIGIN      
        1 gcacccgtgc cgcgacctcc ggcggatagt gcttcgagaa gatccccatc gcgtacttcc
       61 ttccgtcttc aggctcccgc tctcggcctt cgtcctcagg ccggtgcgat cgagcgccag
      121 gcctccagcg cgaacgcgac accgtcatcg tccacgtcga cgtgggtcac catgcggacg
      181 tgcgtgtcca cgtgggtcac cccgacgccg agggccgcga gccgctcgac cgtttcgagc
      241 accggcatcc ccacagcctc tgtgtcgacg aagaccatgt tcgttgcaac ctgatccggg
      301 tcgatcgagt tgggcagcat ctcccccacc gtcgtcgcca gcacctgggc acgctcatgg
      361 tcctcatgga ggcgctgcga tccctcttcc aacgcgatga tgcccgcggc agccatcatg
      421 ccggcctgcc gccaggcgcc gccgaacaag atcgagagcc tgcgggcttc gcggatcatg
      481 tccgaaggtc cgcagatcat cgacccgatc ggtgcgccca gtcccttcga cagacagaac
      541 atgagcgtgt ccacctgacc ggtccactcc gtgacgtcga cgcccgccgc cgcggccgcg
      601 ttgaataccc tggctccatc catgtgcatg ggcacgccga cctcagacgc gaccgtgcgc
      661 accttcgcca gctcatccac cggcatcacc gtgccgcccg cgccaccgac cgtgttctcg
      721 acgcacaaca gatcgatgat ctctacgtcg tagtagctgt cgggttcgag caacgagcgt
      781 gcatgcgaag cgtcgatcca gccgcgggcg ccggggtcca ccgtgcggaa cgcgatgccg
      841 gacagtgccg cggaggtcat gagttctgtc gtggctacgt gcgcagcggc tgaggacgcc
      901 accagatggc cgttcgttcg cacgtgcagc gccagcgcga tctggttggc catcgtgccg
      961 gtgggcacga agagcgccgc ctgcgtgccg gtgcgttcgg cagccagctc ttggagccgg
     1021 ttggcggtgg ggtcgtcgcc gtaccaggca tcacccactt ccgcgtcggc catggccctg
     1081 cgcatcgcag gagacggcgt ggaaacggtg tcggaacgaa ggtcgacgcg ataggtaccc
     1141 atacgcgcga ttctaccggc gctccgtcat cgcccggcat cgggccgcct cggcacgcct
     1201 acccgaagaa gacttccgcc tcgcggtaga gctcgagcgg cacgaccttt cgctcggcga
     1261 cggccgaggt cagcgggacg tcgatgatct cctgcgctcg gcgtgcgacc atccggcccc
     1321 aaccaccagc gttggcgaga gcgacggcac ccaggcccat gcgggtcgcg agcacacggt
     1381 cgaacgccac cggggagcca ccccgctgta catggcccag gatcgtgacg cgggtctgga
     1441 acccggttcg ttcctcgatc tcgggcgcga tgtgggtgga gacacctccc agtcggggga
     1501 atccattctc atccagctca ggttcgggca ggacgagggt accctcggcc gggatggctc
     1561 cttcagctac caccacgatc gagaacgagc tgcgttgcat atgacgcatg cggagccgat
     1621 ggcagacctc gtcgatctcg aacggttgct cggggatcaa gatgacgtcg gcgccaccgg
     1681 ccataccggc gtacaccgcg atccaccccg cgtgacggcc catcacttcc accaacatga
     1741 cgcggtcgtg gctctcggca gtcgagtgca accggtcgat cgaatcggtc gccacctgga
     1801 ccgcggtgtg gaagccgacg gtgatgtcgg tgccgcccag gtcgttgtcg atcgtcttcg
     1861 gcacgccgac caccgggaga ccttcatcgg ccaactgggc ggcggcgccc agcgtcccct
     1921 cacctccgat cgcgatcaac ccgtccagtc cacggcgttc gaaggtggcg cgaacgatcg
     1981 cggggccgtc ctcgacacgc atcgggttga cgcctgagct ccccaggatc gtgccgccgc
     2041 ggtgaaggat gccggtgact tcggggatgc cgatgtcgat cgactcgtcc tccaacacgc
     2101 cccgccatcc atcacggaag cccacgaacg aatggccgtg cacaccgacg cccttgcgga
     2161 ccgaggctct gatcacggcg ttcaatccgg ggcagtcgcc gccgccggtg aggatgccca
     2221 gtcgcatgca tccaccctac ccgcggcccg gaccggggcc gggagcctcg cacccgttcg
     2281 gtaggatgca ggccatgagc ttcatcgcta cgtcccggct cgcctcgtcc ttctggcccg
     2341 agatgcccgg tttcctcgag aagttcttcg tgggcctgct tgcgctgctg gtcgttgcgg
     2401 tcggcgtatt cgcgctcttc ctcatcgcca atcagttcag gaacccgggc cgccggccgg
     2461 gcgaaggccc gtaaggacag aactcagccc ttcacgacct gggtgccggc ctcgccggag
     2521 agcgccgccg gcgcgtctgc gagttccgcg atcaccgcac ggccgccgga ccgggcgaac
     2581 gtggcggccg cctccacctt cggtcccatg gacccggcgc cgaactcccc cttcttcagc
     2641 agtgcggtcg cttccttcac cgtgaggcgg tcgatgtcct cgggcatgaa cgaaccgtag
     2701 ccccgttgca ccttctccac gtcggtgagg atcaacaagg tggaggct
//
LOCUS       DATDBZ010000583         6525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1422091, whole genome shotgun sequence.
ACCESSION   DATDBZ010000583 DATDBZ010000000
VERSION     DATDBZ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422091"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..135)
                     /locus_tag="VIX62_13440"
     tRNA            complement(63..135)
                     /locus_tag="VIX62_13440"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(99..101),aa:Glu,seq:ttc)
     gene            219..386
                     /locus_tag="VIX62_13445"
     CDS             219..386
                     /locus_tag="VIX62_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861280.1"
                     /translation="MMRIVLEWLGLVGPDPARKEPVAVPAWGPYAAAAFVAIAATVLG
                     VLVKVLITATV"
     gene            complement(370..750)
                     /locus_tag="VIX62_13450"
     CDS             complement(370..750)
                     /locus_tag="VIX62_13450"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY5861281.1"
                     /translation="MRHFLVEGPRNRTWTFEVPTRRRERVRGLRDRTWLAPDHGMLFE
                     RCRSVHTVGMRFVIVVASLDREGRVIRVRTMRPRRLMVPRPGVRSVLECRADADVRAG
                     DRFDVVRGLSAAGGKPPAALRPSR"
     gene            complement(784..1794)
                     /locus_tag="VIX62_13455"
     CDS             complement(784..1794)
                     /locus_tag="VIX62_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861282.1"
                     /translation="MSPGNVQAERATIAARGTRVIAGWVTQTKVTGYQPGARRAFWVR
                     RSRDQGKHWGAPIRLSAAKGRVDYPRVALAGSRAFAVWTDAKTGNIRLATSTDGGAHW
                     STKTIGSTNAHAGRPREGRSGLPDLGASGQNLAITWYTNDQGRQVVLTSANGGADLDA
                     GSTPIVLANTSPPNGIRYAGAAGASDEASNRVAVAYSTPSGIETRVWDGVSLGPGSTV
                     VSWPLAADGQTYASGYGPAVLPSGSDDLMVAFAGCRARPIGNPCDTFDPAARVDIVVV
                     TSNDDGGDWGSANAITDSAKQPYRTNAEPSLALSGSRFRLSFDRYQSTFKDYRVWIRS
                     SA"
     gene            1859..2140
                     /locus_tag="VIX62_13460"
     CDS             1859..2140
                     /locus_tag="VIX62_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861283.1"
                     /translation="MRGPWFVAQAPFGGGQSLAQADNTNAEEEASALPGDQLKGAAAV
                     RGSLQVAPAEADVAPSEISSDAMIAGAAPYRRARGEIIAGILDHGGWLG"
     gene            2174..2839
                     /locus_tag="VIX62_13465"
     CDS             2174..2839
                     /locus_tag="VIX62_13465"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEY5861284.1"
                     /translation="MTPDALAPIFFLSAGLLAAAGAAKLARPRPTAQALLDVGWPASD
                     GIARGVGTVEIAVGAGALLIGGLVPALAMAGVYLAFAGFLGFVLWVHPDAGSCGCAGT
                     KAVPPSRLHLVLNLIAALSGVAFAMVDGPGLVTWIGDLGTATLVVVPGVVLAGWLVLV
                     AVTEAPEAWRAWIPPAPHEGHEHEPRDHHARAEEALASAGIGPGHPSLWPGVEPPPGD
                     EVG"
     gene            2836..3423
                     /locus_tag="VIX62_13470"
     CDS             2836..3423
                     /locus_tag="VIX62_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861285.1"
                     /translation="MTARVLHGAMRLASRRGPGRGRRPARIAAAAAAGVVAPLAYASR
                     PVTADALIRRTCSSCPAGSKCCGGYTAFCCTLPRGSNLGCPPNTFVGGWWQCNYGSSG
                     LCNTTNIRYYLDCSANPGTQCQGGCHCGSNDCQNFKTCCVVFRYGQCNTQIPYVTPIM
                     CRLITCIIPCRIDCLNCNCSGAVDQFTCTHNAGCL"
     gene            3427..4122
                     /locus_tag="VIX62_13475"
     CDS             3427..4122
                     /locus_tag="VIX62_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861286.1"
                     /translation="MDATSVLTVVVVIETVVLALLAVVVVSLLRSHAEVLRRLPEPDG
                     DESHEHGRTMVRLERGTPEATVDPNTTAVPSYLPAPNGAVTQANDIGGRTLQGDTVAI
                     TMATGRPTLVAFLSSGCLTCRSFWDGLRPEVRRPLPGQARLVVVVKDREFESPSKLLE
                     LAPADVLVVHSSEAWEAYGITMSPYFCFVDGTDAIVRSEGAATSWEQVRSLLTDAIAD
                     EELARREAEMRTP"
     gene            4229..4825
                     /locus_tag="VIX62_13480"
     CDS             4229..4825
                     /locus_tag="VIX62_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5861287.1"
                     /translation="MLGSITPLGERGRGSRWWLTVSAYLLGSILAGLITGAVFGWAGS
                     LVVSGLELEMRFALLAFVVLLGLALDLGALGLHLPTIHRQVDEDWRTSYRGWVWGLGY
                     GLQLGAGVVTIVTSSTVYSTWFAAFLTADLRAGAIIGLVFGLARSLPVFAVARVRRPD
                     QLLRVDGILGRLAGPARKAALAGGAALAALSLAGAARW"
     gene            4819..5295
                     /locus_tag="VIX62_13485"
     CDS             4819..5295
                     /locus_tag="VIX62_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861288.1"
                     /translation="MVTITDHGLSLRVPSGWEARMWVPDLPPPAINLPVVRLTNDAMP
                     VTRNTYAVEESEKLRRSQVVASLVEFDPSLADVGLYAPQGVPEFGVDDLDPRALQRAQ
                     EGRAGLQRFFSVNGRAFSLYVMARQGSGLTRIMQELHDLLVTLTVDPMTAGEEGSP"
     gene            5292..5885
                     /locus_tag="VIX62_13490"
     CDS             5292..5885
                     /locus_tag="VIX62_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861289.1"
                     /translation="MNVWGWVAIGLLAAVVLWLAFALAGAVRELAALRGRVVALEEES
                     GPVHLADGLPVGAVAPSWQLAEGGGAAVSSSAMRGKRHLVLFADADCRACDALVPEVI
                     EAASSDRLIPVVIVGRGVASATPAAWVPAAGRVIVGVEQGSEVSEAFRTEVSPHAFVI
                     DEGGFVTAQGGPLTLDDVRALIAEAEGLRIVTGAADG"
     gene            complement(6277..6351)
                     /locus_tag="VIX62_13495"
     tRNA            complement(6277..6351)
                     /locus_tag="VIX62_13495"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6314..6316),aa:Lys,seq:ttt)
BASE COUNT          861 a         2196 c         2408 g         1060 t
ORIGIN      
        1 tgacagggag gtgaggacgg ccggactcct ctacgggcgc atatggtgat gcgtgtgctg
       61 cgtgccccca acgggattcg aacccgtgct tccgccttga aagggcggcg tcctaggccg
      121 ctagacgatg ggggcgtcaa cccgaggcta ccggcctgga aggcagccga cagagtatag
      181 ccactcaacc gaggcggctc agcggccgaa cattgggcat gatgcgcatc gtcttggagt
      241 ggctcggtct ggtgggacct gacccggctc gtaaggagcc cgtagcggtc cccgcgtggg
      301 gtccgtacgc cgcggcagcg ttcgtcgcca tcgcggcgac ggtcctgggg gtcctcgtga
      361 aggtgctcat caccgcgacg gtctgagtgc cgccggaggc ttccccccgg cggcgctcag
      421 ccctcgcacc acgtcgaagc ggtcgcccgc tctcacgtcg gcgtccgcac gacactcgag
      481 caccgatcgc acccctggcc tgggcaccat gaggcgtcgg ggccgcatcg ttcgaacgcg
      541 gatcacccga ccctcacggt cgagcgatgc cacgacgatc acgaacctca tgcccaccgt
      601 gtggaccgac ctgcaccgct cgaacagcat cccgtggtcg ggggcgagcc acgtccggtc
      661 gcggagcccg cggacgcgct cgcgccggcg cgtgggcacc tcgaacgtcc aggtgcggtt
      721 ccgcgggccc tcgaccagga agtggcgcat ccaaccggac ggtagcccgg cccaccgaca
      781 cggctacgcc gaggaccgga tccagacgcg gtagtccttg aacgtcgact ggtaccggtc
      841 gaagctcagc cggaacctcg acccgctcag ggcgagcgag ggttctgcgt tggtgcggta
      901 gggctgcttg gcgctgtcgg tgatggcgtt ggccgatccc cagtcgcctc cgtcgtcgtt
      961 cgaggtgacc accacgatgt cgaccctcgc ggccgggtcg aacgtgtcgc acgggttgcc
     1021 gatcggccta gcacggcatc ctgcgaaggc caccatgaga tcatccgacc cactgggcag
     1081 gacggcgggg ccgtagccgc tggcgtaggt ctgcccgtcg gcggccaggg gccacgagac
     1141 cacggtcgat cccggtccga gggatacgcc gtcccacacc ctggtctcga tccccgacgg
     1201 ggtcgagtag gcgacggcca cgcggttgga ggcctcgtcg ctcgcgccgg cggctcccgc
     1261 gtaccggatc ccgttgggcg ggctcgtgtt cgcgagcacg atcggggtgg aaccggcgtc
     1321 gagatcggca ccgccgttcg ccgaggtgag gaccacctga cggccctggt cgttcgtgta
     1381 ccaggtgatg gcgaggttct ggccgctggc gccgaggtcg gggaggccgg agcgaccttc
     1441 gcgaggtcgt ccggcgtgag cgttcgtcga gccgatcgtc ttggtcgacc agtgcgcgcc
     1501 gccgtcggtg ctcgtggcca gccggatgtt cccggtcttg gcatcggtcc agaccgcgaa
     1561 cgcccggctg ccggccagcg ccacccttgg atagtcgacc cgccccttcg cggccgacag
     1621 ccggatcggc gctccccagt gcttgccctg gtcgcggctc cgtcgcaccc agaacgcgcg
     1681 ccgagcgccg ggctggtagc ccgtgacctt ggtctgggtg acccagccgg cgatcacgcg
     1741 ggtgccgcgg gcggcgatcg tggcccgctc ggcctggacg ttccccgggc tcacgcggcg
     1801 agggttgctc cacttcaggc cgaccgtgtc gctcctggcg cgttggacga agacccccat
     1861 gcgcgggccg tggttcgtcg cgcaggcgcc cttcggcggc gggcagtcgc tggcccaggc
     1921 tgacaacacg aacgccgagg aggaggcgag cgccttaccc ggcgaccagc tgaagggggc
     1981 cgcggccgtg cgtggctcgc tccaggtggc ccccgccgag gccgatgtcg ccccgagcga
     2041 gatcagcagc gacgccatga tcgcgggtgc agcaccgtac cggcgcgctc ggggggagat
     2101 catcgcgggc atcctagacc acggcgggtg gctcggttga ccacccccga gcgcgccggt
     2161 agcctcgacg aacgtgacgc ccgacgcgct cgcccccatc ttcttcctgt ccgccgggtt
     2221 gctcgccgcc gccggagccg ccaaactcgc ccgcccccgt cccaccgccc aggcgctcct
     2281 cgacgtggga tggcctgcct ccgatgggat cgcccgcggg gtgggaaccg tcgagatcgc
     2341 cgtcggtgcc ggcgcgctgc tgatcggcgg cctggttccg gccctggcga tggcgggcgt
     2401 ctatttggcc ttcgcggggt tcctggggtt cgtgttgtgg gtgcatcccg acgcaggctc
     2461 ctgcggatgc gcgggcacga aggccgtccc tccgagccgc ctgcacctgg tgctcaacct
     2521 gatcgcggcg ctctccgggg tcgcgttcgc gatggtcgat ggacccggct tggtcacctg
     2581 gatcggcgac ctgggaaccg cgacgctcgt ggtcgttccc ggggtggtcc tggccgggtg
     2641 gctcgtcctg gtcgccgtga ccgaagcgcc ggaggcctgg cgcgcctgga tcccgcccgc
     2701 gccccacgaa gggcacgagc acgagccgcg tgaccatcac gcccgggcgg aagaagccct
     2761 cgcatcggcg ggcatcgggc cgggccaccc gagcctgtgg cccggcgttg agccgccccc
     2821 cggggacgag gtcggatgac ggcgagggtc ctgcacggcg ccatgcgcct ggcctcccgt
     2881 cgcggtcccg gccgcggtcg caggccggcg cgtatcgctg ccgcggcggc tgccggtgtc
     2941 gttgcgccgc tcgcctacgc gagccggccc gtcaccgcgg atgccctgat ccgccggacc
     3001 tgctcgtcgt gtccagccgg cagcaagtgc tgcgggggtt acacggcgtt ctgttgcacg
     3061 ctcccccgtg ggtccaacct gggatgtccg ccgaacacgt tcgtcggcgg ctggtggcag
     3121 tgcaactacg gcagctcggg cctgtgcaac acgacgaaca tccgctacta cctcgactgc
     3181 tcggcgaacc cgggcacgca gtgtcaggga gggtgccact gcggcagcaa cgactgtcag
     3241 aacttcaaga cctgctgtgt ggtgttccgc tacggccagt gcaacacgca gatcccgtac
     3301 gtgacgccga tcatgtgccg gctgatcacg tgcatcatcc cgtgccggat cgactgcctg
     3361 aactgcaact gctcgggcgc cgtcgaccag ttcacctgta cgcacaacgc ggggtgtctg
     3421 tagcgcatgg atgccacctc ggtgctcacc gtcgtcgtcg tgatcgagac ggtcgtgctg
     3481 gcgctgctcg ccgtggtggt ggtctcgctc cttcgcagtc acgccgaggt ccttcgccgg
     3541 ctgccggaac ccgatggaga cgagtcgcac gaacacggcc ggaccatggt gcggctcgag
     3601 cgcgggaccc cggaggcaac ggtcgacccg aacaccaccg cggtgccgtc ctaccttccg
     3661 gcgcccaacg gcgccgtgac gcaggcgaac gacatcggcg gccgcacgct ccagggcgac
     3721 acggtggcga tcacgatggc caccggccgg cccacgctgg tggcgttcct ctcgagcggc
     3781 tgcctgacct gtcgctcgtt ctgggacggg ctcaggcccg aggtgcggcg tccgcttccc
     3841 ggacaggcac ggctcgtcgt ggtggtgaag gatcgggagt tcgagagccc gtcgaaattg
     3901 ctcgaactcg cgcccgccga cgtgctcgtg gtgcattcct ccgaggcgtg ggaggcctac
     3961 ggcatcacga tgtcgccgta cttctgcttc gtcgacggca cggacgcgat cgtgcgctcc
     4021 gagggggccg cgacgtcgtg ggagcaggtg cggtcgttgc tgaccgacgc gatcgccgac
     4081 gaggaactcg cgcggcggga agccgagatg cgaacgccat gacggagcca aggacggaga
     4141 ggacctgatg gaaccgttga cggtggtggg gatcggactg gccgtggtgg ccgggctgcg
     4201 cgccacctgg tcgccctgag gcgtatcgat gctgggaagc atcactccgc tcggggagcg
     4261 gggccggggt tccaggtggt ggctcacggt gagcgcctac ctgctgggct cgatcctggc
     4321 cggtctcatc acgggagcgg tgttcgggtg ggccgggtcc ctcgttgtga gtggcctcga
     4381 gcttgagatg cggttcgcgc tgctggcgtt cgtggtgttg ctgggactgg cgctcgatct
     4441 aggagcgctg gggctgcacc tgcccacgat ccatcggcag gtcgatgaag actggcgcac
     4501 ctcctaccga ggatgggtct ggggactggg ctacggtctg caactcggcg cgggcgtggt
     4561 gacgatcgtg acgagttcca ccgtgtactc gacgtggttc gcggccttcc tcacggccga
     4621 tctgcgtgcg ggggcgatca tcgggctggt gttcgggctc gcgaggtcgc tgcccgtgtt
     4681 cgccgtggca cgggtccgcc ggccggacca gttgctccgg gtcgacggca tcctcgggcg
     4741 gctcgccggc ccggcgcgca aggcggcgct ggcaggcggg gcggcactgg cggcgctctc
     4801 gctcgcggga gcggcgcgat ggtgacgatc acggaccacg gtttgtcgct ccgcgtgcca
     4861 tcgggttggg aagcccgcat gtgggtgccc gacctgccgc cgccggcgat caacctgccc
     4921 gtggtgcggc tcacgaacga cgccatgccg gtcacgcgca acacgtatgc cgtcgaggaa
     4981 tccgagaagc tccgccgctc gcaggtggtg gcctcgctgg tcgagttcga cccctcgctc
     5041 gccgacgtgg gtctctacgc gccacaaggc gtgcccgagt tcggggtgga tgacctcgat
     5101 ccccgagccc tgcagcgggc acaggagggg cgcgccgggc tccagcggtt cttctcggtg
     5161 aacggcaggg ccttcagcct gtacgtgatg gcgcggcagg gttcgggcct gacgcgaatc
     5221 atgcaggagc tccacgatct tctggtgacg ctcacggtcg atcccatgac cgcgggcgag
     5281 gaggggtcgc cgtgaacgtg tggggatggg tggcgatcgg gttgctggcg gccgtcgtcc
     5341 tgtggctcgc gttcgcgctc gccggcgccg tgcgggagct ggccgccctc cgtgggcggg
     5401 tggtggccct cgaagaggag agcggaccgg tgcacctcgc cgatgggttg cccgtgggcg
     5461 cggtcgctcc gtcgtggcag ctcgccgagg gtggcggcgc ggcggtgtcg tcctccgcga
     5521 tgcgggggaa acggcacctg gtcctgttcg ccgacgccga ctgccgggct tgcgacgcgc
     5581 tggtgcccga ggtgatcgag gccgcgtcga gcgaccgcct gataccggtc gtgatcgtgg
     5641 gtcgaggggt ggcgagcgcg acgcctgcgg cgtgggtgcc cgccgccggc cgggtgatcg
     5701 tgggggtgga acagggctcc gaggtctccg aggcgttccg caccgaggtg agtccacatg
     5761 cgttcgtgat cgacgagggt gggttcgtca cggcgcaggg cggcccgctg acgttggacg
     5821 acgtacgggc gctgatcgcc gaggccgaag ggctgcggat cgtgacgggg gcggccgatg
     5881 gctgagcgat ggggcacgcc gatgcccctc acccggcgcg ggttcatcac gcgaacggcg
     5941 ggcgcggcct tggctgggtt ctccgtggtg gccggagccg gcgggggccg ggcctccgcg
     6001 catgtgaagc cgccgccgtg ccgtgtgcga tgcgagccga tcagcaaggg cggatgtgcg
     6061 tgcggtggcc atttctaccg gtgctcgggg tgcggcaggt cgttccacgc ctgcatcgaa
     6121 cgcgaccgtt tcgggtggtt ctgccttcgt cgcaagtgct gaggcggccc gcaaggatcg
     6181 ccgataccgg atgcgatatg cgatcgaggt cggcggccga ggagtgattc cctctctcgg
     6241 gatcgggatg tccggcaaca cggatatccg cctgcgtggg cccggcagga ttcgaacctg
     6301 cgacacgagg gttaaaagcc ccctgctcta accaggctga gctacgggcc cgatggctcc
     6361 gaaggagtct acgaacctca gcccgggtag cggttcgtga tcggcatccg ccggtctcgc
     6421 ccgaacgcct tcggggtgat cttcacgcct ggcgccgcct ggcgacgttt gtactcgttg
     6481 cggtcgatca tcgccacgac gcgctctacg atcgagcgtt cgaac
//
LOCUS       DATDBZ010000584         3842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1422531, whole genome shotgun sequence.
ACCESSION   DATDBZ010000584 DATDBZ010000000
VERSION     DATDBZ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422531"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VIX62_13500"
     CDS             complement(<1..995)
                     /locus_tag="VIX62_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5861290.1"
                     /translation="MEAPITRYARSADGTFVAYQTFGSGEDVLVIMPFISHLELFWED
                     PDVGGMLRGLAGSARVIAMDQRGMGLSDRLQGDPDLETRMDDVRAVIEAAGSRLPTLW
                     GLGVDGGALCAMFAATYPERVRGLILWPGSASGRRAPDYPWAETDHALRETELHIASG
                     WGDEDGTRLLLSDLGLTSILGDDVAVHRWAKIFRYAASPGDAISFNSMWDDLDFRSIL
                     PAIRVPTLIQSRNNPEEARWVTGQIPGASLEEFHDSPDYPPYVGDTAAVLASVRRFVA
                     GLRSDEVEFDRVLATVLFTDIVGSTDLAARLGDRAWHGLLEQHHALVRRELARFRG"
     gene            1116..1523
                     /locus_tag="VIX62_13505"
     CDS             1116..1523
                     /locus_tag="VIX62_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY5861291.1"
                     /translation="MAGTYRVIVAKPGLDGHDRGAKVVARALRDAGFEVIYTGLHQTP
                     EQIAETAIQEDADAVGLSCHSGAHMTLFPKVVQQLRERGAGDIVVFGGGIIPADDIPT
                     LQAEGIEKVFTPGANTGDIATWLRERFAESTSS"
     gene            1549..2694
                     /gene="sucC"
                     /locus_tag="VIX62_13510"
     CDS             1549..2694
                     /gene="sucC"
                     /locus_tag="VIX62_13510"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY5861292.1"
                     /translation="MDLYEHQGKELFRAQGIATPRGIVATTGEQAAEATRELGGKSVV
                     KIQVQVGGRGKGGGVVLVDSPERAAEEASRMLGTDFQGHEVTQVLVEELLPIAQEFYT
                     SILLDRSTGDYLAMMTAEGGMDIEDLARTRPEALRRVHIDAMLGLRAFHVRELTGTLP
                     QEAREGASDVLRRLYTLLQEADATLVEVNPLVLLEDGRVVALDAKVTVDDNALFRHED
                     IEAMKSAFPIDPVQARANEKDLQYVKLDGNVGIIGNGAGLVMSTLDVVAQAGAKAANF
                     LDVGGGASADQMATSLEVVLSDPAVKAVFINIFGGITRCDLVAKGVLGALDRIEASVP
                     IVVRLDGTNAEEGRAILADAAHPRIVAAATMLEAAERVAGFANGEAA"
     gene            2691..3560
                     /gene="sucD"
                     /locus_tag="VIX62_13515"
     CDS             2691..3560
                     /gene="sucD"
                     /locus_tag="VIX62_13515"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY5861293.1"
                     /translation="MSVLVGAETRLVVQGITGHEGTFHTLRDRAYGTNVVAGVTPGKA
                     GQDVEGIPVYDTVADAVREQGADTAMIFVPPRFAAEAILEAADAGVPLAVCITEGIPA
                     KDMAHVHSYLQGRSTTLVGPNCPGVITPGVANVGIIPGEICMPGRVGLVSRSGTLVYQ
                     IVHELTQRGLGQSTCIGMGGDPVHGIGFIESLELFQQDPDTDLVVMTGEIGGDDEERA
                     AAYIAEHVTKPVVGYIAGFEAPPGKRMGHAGAIVTGSKGTAAAKAEALEAVGVRVART
                     PTQVAELVVEALG"
     gene            3557..>3842
                     /locus_tag="VIX62_13520"
     CDS             3557..>3842
                     /locus_tag="VIX62_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861294.1"
                     /translation="MSDPAPAAGHPEAAVDRPLSADLRLAAPVAARGGLLVAVAAIVL
                     GQLPPLLVNLLGGGLAVSTQVRMGWLYTAASNAVSIRIEGLSAAAGELEPL"
BASE COUNT          611 a         1315 c         1345 g          571 t
ORIGIN      
        1 ccgcgaaacc gagcgagttc ccttcgaacc agtgcgtgat gctgttcgag cagaccgtgc
       61 catgcgcgat cgcccaggcg tgctgccaag tccgtcgaac cgacgatgtc ggtgaacagc
      121 acggtggcca gtacgcggtc gaactcgacc tcgtcggacc gcagccccgc aacgaaccgt
      181 cgcaccgagg ccaggaccgc ggcggtgtca cccacatatg gtgggtagtc aggggagtca
      241 tggaactcct cgagggacgc gcccgggatc tgtccggtga cccagcgcgc ttcctcggga
      301 ttgttccggc tctggatcaa ggtcggcacg cggatcgccg gcaggatgga acggaagtcg
      361 aggtcgtccc acatgctgtt gaacgagatc gcgtcgcccg ggctcgcggc atatcggaag
      421 atcttggccc atcgatgcac cgccacgtcg tcgccgagga tgctcgtgag cccgaggtcg
      481 ctgagcagca gcctcgtccc gtcctcgtcg ccccagccgg aggcgatatg gagctccgtc
      541 tcgcgcaggg catggtcggt ttccgcccac gggtagtcag gcgcccggcg accgctcgcg
      601 ctgccaggcc acaggatcag tcctcgcacc cgttcggggt acgtggcggc gaacatcgcg
      661 cacaaggcgc ctccgtcgac gcccaatccc cacagggtgg ggagccgtga gcccgccgct
      721 tcgatcacgg ctcgcacgtc atccatccgg gtctcgaggt ccggatctcc ttgcagccgg
      781 tcggacagac ccatcccgcg ctgatccatg gcgatcacgc gggcggatcc cgcgagccct
      841 cgcagcatgc cgccgacgtc ggggtcttcc cagaacaact cgaggtgcga gatgaacggc
      901 atgatcacga ggacgtcctc gccgctcccg aaggtctggt aggcgacgaa ggtcccgtcg
      961 gcactgcgcg catagcgggt gatcggggct tccacgttcc cgatactgtg ccagttcgtg
     1021 ccgtgcccgc aacggcggcc ccgtgccgac acgtggttcg ggtgcaggct cctgggccgc
     1081 agcgccatcg ggttcgttcg ttacacttcg cccacatggc cggcacctac cgcgtgatcg
     1141 tggcgaagcc gggcctcgac ggacatgacc gcggggccaa agtggtggca cgcgccctgc
     1201 gcgacgccgg gttcgaggtg atctacacgg gcctgcacca gacccccgag cagatcgccg
     1261 agaccgcgat ccaggaggac gccgacgccg tcgggctctc gtgccattcc ggggcccaca
     1321 tgacgttgtt ccccaaggtg gtgcagcagc tccgcgagcg cggggccggg gacatcgtgg
     1381 tcttcggtgg cggcatcatc ccggccgacg acatccccac gctgcaggcc gagggtatcg
     1441 agaaggtgtt cacccccggg gccaacaccg gcgacatcgc cacctggctt cgcgagcggt
     1501 tcgccgagtc gacgtccagc tagtccaaga cgactcacag ggagctccat ggatctgtac
     1561 gagcatcaag gcaaggagtt gttccgcgcc cagggcatcg ccacaccgcg cgggatcgtc
     1621 gccaccacgg gggagcaggc ggccgaggcc acccgcgaac tgggcggcaa gagcgtcgtg
     1681 aagatccagg tgcaggtcgg tggccgcggc aagggcggcg gcgtggtgct ggtcgactcg
     1741 cccgagcgtg ccgccgagga agccagccgc atgctcggca ccgacttcca aggccatgag
     1801 gtgacgcagg tgctggtgga ggaactgctg ccgatcgctc aggagttcta cacctcgatc
     1861 ctgctggacc gttccaccgg cgactacctc gcgatgatga ccgccgaggg cggcatggac
     1921 atcgaggatc tcgctcgcac gcggcccgag gcgcttcgcc gggtccacat cgacgcgatg
     1981 ctggggctcc gcgccttcca cgttcgcgaa ctcaccggca cattgccgca ggaggcgcgc
     2041 gaaggcgcgt ccgacgtgct gcgccgtctc tacacgctgc tccaggaagc cgacgccacc
     2101 ctcgtcgagg tcaacccgct cgtgctgctc gaggacggtc gcgtggtcgc gctcgacgcg
     2161 aaggtcaccg tggacgacaa cgcgttgttc cgccacgagg acatcgaggc gatgaagtcg
     2221 gcgttcccga tcgaccccgt gcaggcgcgc gccaacgaga aggatctgca gtacgtgaag
     2281 ctcgacggca acgtcgggat catcggcaac ggggccggcc tggtgatgtc cacgctcgac
     2341 gtcgtggccc aggcgggtgc caaggccgcg aacttcctcg atgtgggcgg cggtgcgagc
     2401 gccgatcaga tggcgacctc gctcgaggtg gtgctgagcg atccggcggt caaggccgtg
     2461 ttcatcaaca tcttcggcgg gatcacccgg tgcgacctgg tagccaaggg cgtgctgggc
     2521 gcgctcgatc gcatcgaggc cagcgtgccc atcgtggtcc gcctcgacgg caccaacgcc
     2581 gaggagggcc gcgcgatcct cgccgatgcc gcgcatccgc gcatcgtcgc cgccgccacc
     2641 atgctggagg ccgccgaacg agtcgccggc ttcgcgaacg gggaggcggc atgagcgtct
     2701 tggtcggggc cgagacgcgc ttggtggtgc agggcatcac cggccacgag ggaaccttcc
     2761 acactctgcg tgaccgcgcc tacggcacga acgtggtggc cggtgtcacg cccgggaagg
     2821 cgggccagga cgtcgaaggg atcccggtct acgacaccgt ggccgacgcc gtgcgcgaac
     2881 agggcgccga caccgcgatg atcttcgtgc cgccccggtt cgccgccgaa gccatcctgg
     2941 aggccgccga cgccggtgtg ccgctcgcgg tgtgcatcac cgagggcatc ccggccaaag
     3001 acatggcgca cgtgcacagc tacctgcagg gacggtccac gacgttggtc gggcccaact
     3061 gccccggggt catcacgccg ggggtcgcga acgtcgggat catccccgga gagatctgca
     3121 tgccggggcg tgtgggattg gtgtcgcggt ccggcacgct cgtctatcag atcgtgcatg
     3181 aactcacgca gcgggggctg ggtcagtcga cctgcatcgg catgggtggc gacccggtcc
     3241 acggcatcgg gttcatcgag tcgctcgagc tgttccagca ggacccggac accgacctgg
     3301 tcgtgatgac cggtgagatc ggtggcgacg acgaagaacg tgccgccgcc tacatcgccg
     3361 agcacgtcac gaagcccgtg gtgggctaca tcgcggggtt cgaggcgccg ccgggcaagc
     3421 gcatgggcca cgccggcgcg atcgtcaccg ggtcgaaggg gacggcggcc gccaaggccg
     3481 aagcgctcga ggcggtgggc gttcgcgtcg cgcgcacccc cacgcaggtg gccgaactgg
     3541 tggtcgaggc gctgggatga gcgatccggc gcctgccgcc ggccaccccg aggcagccgt
     3601 cgaccggcca ctctccgccg acctccgcct cgccgccccc gtcgcggcgc gtggcggcct
     3661 gctggtggcc gtggccgcca tcgtgctcgg acagctcccg cccctgttgg tgaacctgct
     3721 cgggggcgga ctggcggtct cgacccaggt gcggatgggc tggctctaca ccgcggcatc
     3781 caacgccgtc tcgatccgga tcgaagggct cagtgccgcg gcgggtgagc tcgaaccgct
     3841 gg
//
LOCUS       DATDBZ010000585         2369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2658734, whole genome shotgun sequence.
ACCESSION   DATDBZ010000585 DATDBZ010000000
VERSION     DATDBZ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658734"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VIX62_13525"
     CDS             <1..343
                     /locus_tag="VIX62_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY5861295.1"
                     /translation="RRFRDEAGGMLVDLPRAPLPAADTPTPVRFLPTWDATLLVHARR
                     TQILPEAFRPKVFHTKTPHSFPTFLVDGQVAGTWRYEDGSISLTVFRTLTRTERRSVE
                     DEAERVTAFME"
     gene            510..>2369
                     /locus_tag="VIX62_13530"
     CDS             510..>2369
                     /locus_tag="VIX62_13530"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEY5861296.1"
                     /translation="MSLLVAIPAIAANADRATRDLGAPDDGSAGSSGEASRLPGATDP
                     LAAAGVSPIRGIDVSHWQGEIDWGRVANAGKEFAYLKSTDDIDYIDETFAFNRAQAKA
                     NGLSVGAYHFARPDPSAGDAVREARWFVQRTNPRPGELLPVLDIETSDGLTQAEMTTW
                     AHRWIEEVRNLTGVTSMVYTSPYGWMERFGDTRRIAREGSPLWVAHWGVTSPLVPAGN
                     WNGNGWVMWQYTSQGHVAGIVGDVDLDRLVGGTLGQLTIRKLTLDVDGDAGVVTSAPA
                     GLGCATTCEKNVDPNATVTLSAVPDEDAYFVGWGGACDGTNPSCTITMQGNRSVTAEF
                     VTDITAPVASLTSPNGFTGPATVEFDEAVRGVSSANLIVRRPGIGPVAVDRTCRSAAE
                     APVPCATGKVRSVDLRPQQPLIPGRSYEAVVNPNGTEPIADLIGNPAETTSLPYEGQA
                     VVDQANIPVDHAWRETQNANALGDSFVVERLPGAELSFDFKGRSLTWHTAMGPAYGRA
                     QVAIDGRPVRVVDLWDAKFHTGVERHFDGLGDGDHTITIQPTGHAAPRATDHLVAVDG
                     FGTVSRGTVRTPITQQRWRTVDAGPAFRGSYAVADLAGAAITMRFDGVGIDLTT"
BASE COUNT          393 a          828 c          778 g          370 t
ORIGIN      
        1 gcggcggttc cgggacgagg cgggaggcat gctggtcgat ctgcctcgcg cgccgctgcc
       61 tgcggcggat acgccgacgc ccgtccggtt cctgcccacg tgggacgcca ccctcttggt
      121 gcacgcccgg cgtacccaga tcctcccgga agccttccgt cccaaggttt tccatacgaa
      181 gacgcctcat tcgttcccga cgttcctggt cgacggtcag gtcgcgggca cgtggcgata
      241 cgaggacgga tcgatctcgc tcacggtgtt ccgaacgctg acccgcaccg aacgccgatc
      301 ggtcgaggac gaagccgaac gggtcacggc cttcatggag tgagggcccc cgggaacgcc
      361 cccagcgttt cagcacggcc ttccgggcgc cgatggggca tgcgaggtac cctgctctct
      421 ccgcgcacgt cctcgtgact tcccctatgc ctgcccacct catcccccac cgacggcgcc
      481 gcctcgcccg ctcctgggcg atagtgctgg tgagcctgct cgtcgcgatc cctgccatcg
      541 cagccaacgc cgatcgcgcg acccgcgacc tcggtgcccc cgatgacggt tccgccgggt
      601 catccggcga ggcctcacgt ctgccgggcg ccacagaccc cctcgcggcc gcgggcgtgt
      661 cgccgatccg cggcatcgac gtgtcgcatt ggcagggcga gatcgactgg ggccgcgtcg
      721 cgaacgccgg caaagagttc gcatacctca agtccaccga cgacatcgac tacatcgacg
      781 agacgttcgc cttcaaccgt gcgcaagcca aggcgaacgg gctgtctgtg ggtgcctacc
      841 acttcgcacg gcccgaccct tcggccggcg atgccgtgcg tgaagcacgc tggttcgtgc
      901 agcggacgaa ccccaggccc ggggaactat tgccggtcct cgacatcgaa acgagcgacg
      961 gcctcacgca ggccgagatg acgacgtggg cgcaccgatg gatcgaggag gtccgcaacc
     1021 tcacgggcgt gacgtccatg gtctacacca gtccgtacgg ctggatggag cgcttcggcg
     1081 acacaaggcg gatcgcgcgg gaaggctcac cgctgtgggt ggcccattgg ggtgtgacct
     1141 caccgttggt gccggcgggc aactggaacg gcaacggctg ggtcatgtgg caatacacga
     1201 gccagggcca cgtggccggc atcgtcggcg acgtcgacct ggatcgtctg gtaggcggca
     1261 ccctcggtca gctgacgatc cgcaaactca ccctggacgt ggacggtgac gccggtgtcg
     1321 tgacgagcgc accggcggga ctggggtgcg ccaccacctg cgagaagaac gtggacccca
     1381 acgctaccgt cacgttgtcc gccgtgcccg acgaggacgc gtacttcgtg gggtggggtg
     1441 gggcgtgcga cggcaccaac ccctcgtgca cgatcacgat gcagggcaac cggagcgtga
     1501 ccgcggagtt cgtcaccgac atcacggcgc cggtcgcttc gctcacctcg cccaatggat
     1561 tcaccgggcc ggccacggtc gagttcgatg aagcggtccg aggggtgagt tccgcgaacc
     1621 tgatcgttcg gcggcccggg atcggtcccg tcgcggtgga tcgcacctgt cgttcggcag
     1681 ccgaagcacc ggttccctgc gcgaccggca aggtccgatc cgtcgacctt cgaccgcagc
     1741 agccgctcat accgggtcgg tcctacgaag cagtggtcaa cccgaacggc accgaaccta
     1801 tcgccgatct catcggcaac cctgccgaga ccacatcctt gccctacgaa ggccaggccg
     1861 tggtcgacca agcgaacatc cctgtcgacc acgcatggcg cgagacgcag aacgcgaacg
     1921 cgctcggtga ttccttcgtg gtcgaacgcc tgccaggcgc cgagctttcg ttcgatttca
     1981 agggacgttc gctcacatgg cacaccgcga tgggtccggc ctacgggcga gcacaggtgg
     2041 ccatcgacgg gaggcccgtg cgcgtcgtcg acctgtggga cgcgaagttc cataccggcg
     2101 tcgagcgcca tttcgacggc ctcggtgatg gcgaccatac gatcacgatc cagccgacag
     2161 gacacgccgc accacgagcg accgaccatc tcgtcgctgt cgacgggttc gggaccgtga
     2221 gccgtggaac cgtgcggacg cccatcacgc agcagcgttg gcgaacggtc gacgcgggcc
     2281 cggccttccg tggcagctat gccgtcgccg acctcgctgg agccgccatc acgatgcggt
     2341 tcgacggggt gggtatcgac ctcaccacg
//
LOCUS       DATDBZ010000586        11040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1215462, whole genome shotgun sequence.
ACCESSION   DATDBZ010000586 DATDBZ010000000
VERSION     DATDBZ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11040
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215462"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VIX62_13535"
     CDS             <1..221
                     /locus_tag="VIX62_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5861297.1"
                     /translation="NIFHGELTVDQLFHMRPAVGYADYRSPIAGLYQASSATHAGGGV
                     TGLPGHHAVREILQDKKKRSKQKKGRRT"
     gene            218..1840
                     /locus_tag="VIX62_13540"
     CDS             218..1840
                     /locus_tag="VIX62_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5861298.1"
                     /translation="MTANRYDAIVIGGGHNGLITAAYLAKHGARTVVLESRHKTGGAA
                     DTMSPWEESPDIKVTTLSYVMSLMPPTIQKDLQLERFGYKVYPQGMGYLPVPSGGSII
                     QHDDEEKTRESIRQFSKKDADAYFEFEAWIGRIADIMGPLLMETPPNLGSKKAKDIKD
                     VAQLGWTLRKEIDPQTVADITRLFTMSATDLLDRWFESPVLKGFMAVNGIIGTWAGPD
                     APGTAYVLMHHSVGDIGDGQIASWGYPEGGMGAVSAACEQAARFFGAEVRVNARVSTI
                     KTENGRARGVVLDGGEELIAPIVVTTCHPKITFLDQLNATELPAEFVNDITHWKTRSG
                     TVKINLALAELPEFTADPGFDPNVHGGAIQILDDVDYLEKAFQEARFGQAATLPFSDT
                     AIPTVFDKTLAPEGTHVMSMFTQWVPQEWAEEDGHEADLEAYADRLIDRVNEVAPNFK
                     GSIKHRQIIGPKQMQDEWGLIGGNIFHGELTVDQLFHMRPAPGYADYRSPITGLYQAS
                     SATHAGGGVTGMPGHHCVREIMKDKALKKLKP"
     gene            1887..3656
                     /locus_tag="VIX62_13545"
     CDS             1887..3656
                     /locus_tag="VIX62_13545"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5861299.1"
                     /translation="MSRDEGLRAVDEAFERLFADGTIPGMAYGVVIAGDLVHARGLGT
                     LHQGEDRVPDADSVFRIASMTKSFTASAVLLLRDEGALLLDEPVGTYVPELAGFRGAT
                     ADSPLITVRHLLTMSSGLPTDDPWGDRLQGLPLDAFAELLGGGFTFAWPPGTAFEYSN
                     LGYGILGRVLTNVSGQEYRDLIRDRLLTPLGMDSTVYEADQVPDARLARGHVHRDGAF
                     VDEPFDPYGALASMGGVFSSVRDLTTWVHGFAEAFPARDDPEGTHPLSRASRREMQQV
                     QRAFDPELGWTSAGAAPSLLAGGYGFGLFVSQDLTLGHVVDHSGGYPGFGSHMRWHPA
                     SGVGVIALGNRTYAPMSRPATEALKALVAAEVAAVGTVVGWDATEAARADVERLLAGW
                     DEALAARRFAFNVDLDEPLALRRTSFEEIRERHGALTRDPDESATRDSAAHLAWWLTG
                     ERGGRVRVEILMTGEPAPRVQSMDVRSVAEPSEALQTLVERVVEVLNHQPPEPSWPPE
                     LVPAGQTDADRDLDGRSLRVAAAAFAPVRRMRPTRGDGNTTVTVPLSGAQGRLELTIA
                     VDDSGALTARTLRPERLPAGRGS"
     gene            3879..4328
                     /locus_tag="VIX62_13550"
     CDS             3879..4328
                     /locus_tag="VIX62_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861300.1"
                     /translation="MRQWELIGITDTVPTPPPPPRSRFANAATIGAAAEGRPVFFWST
                     DTALRLRTVTGAATEVIGMPFSKTEGRDLIAVFGMEGPHLALLDAHVAALNGETAEFT
                     LEGQRATVRCQVAPTHDALERVTGTFCLAVDVEKVDLREVVEHAAVA"
     gene            complement(4371..5645)
                     /gene="hemL"
                     /locus_tag="VIX62_13555"
     CDS             complement(4371..5645)
                     /gene="hemL"
                     /locus_tag="VIX62_13555"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY5861301.1"
                     /translation="MTRSEDLFARARTVIPGGVDSPVRAFGGVGGTPVFFDRGEGAFL
                     IDVDGNRYVDLVQSWGALLFGHARPEIVEAALRAAAKGTSFGTPTEGEVELAQRIVDA
                     VPGVEQVRLVNSGTEATMSAIRLARGATGRDLVVKFEGCYHGHSDSLLAKGAGSGLAT
                     LGIPSSPGVTEGSAKDTLTAPFNDLEAVRAIFAERGGEVAVVAVEPVAANMGVVPPAP
                     GFLEGLRELCTEHGALLLFDEVITGFRIAYGGAQSFYGVTPDLTALGKVMGGGFPCAA
                     FGGPATLMERLAPTGPVYQAGTLSGNPVAVAAGIAALDLARTTDPYPGLEATADVLTD
                     GIAAAFAAIDIPVTINRAGSMFSVFFAGGPIRNFSDASAADHERYARLFHHLLDAGVY
                     LPPSGYELWTLSTTHGPEETERILGAVSSFRG"
     gene            complement(5658..6650)
                     /gene="hemB"
                     /locus_tag="VIX62_13560"
     CDS             complement(5658..6650)
                     /gene="hemB"
                     /locus_tag="VIX62_13560"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY5861302.1"
                     /translation="MSYPASRPRRLRRTPALRALIRETSLDAGDLIAPLFAKEGIDEP
                     VAIASMPGQFQHTLESLRKEAVEIAETGVRAFILFGVPARKDAEGSEAWNPSGIGQQA
                     IAALRTEFGDDAVVMSDLCLDEYTDHGHCGVLTADGTDVDNDETVERYRRIAIAQAEA
                     GTHLVGPSGMMDGQVAAIRDALDGAAHTDVGIIAYAAKFASAFYGPFREAAECAPSFG
                     DRTGYQQDPANADEALREIRTDIDEGADIVMVKPAVPYLDIVSRAKQETAVPLAAYHV
                     SGEYSMLKAAAANGWLDERRSVLETLTSIRRAGADLILTYHAKDAAGWLASDRP"
     gene            complement(6677..7453)
                     /locus_tag="VIX62_13565"
     CDS             complement(6677..7453)
                     /locus_tag="VIX62_13565"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY5861303.1"
                     /translation="MSPRSPKPLAGRTILLTRPADQSASLVKLLDQRSAHTIVAPAIE
                     VRPIRSAALTTALRDLADGDFSWITITSRATVEMLASRLGSPADVRTKVAVIGDGSAE
                     AFRRWAGRAPDLVPPTFTTVGLARAFPRGEGRVLCARADIAPEGLEDALASKGWRPTR
                     VDAYRTRMPRTLPAEAREALRAGTVDAVTFTSASTVRGFVGALGPVKGNPRVVCIGPV
                     TANEARAHGLTVSAVANPHTIDGVVAALERVFTPRRRAGS"
     gene            complement(7450..8343)
                     /gene="hemC"
                     /locus_tag="VIX62_13570"
     CDS             complement(7450..8343)
                     /gene="hemC"
                     /locus_tag="VIX62_13570"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY5861304.1"
                     /translation="MRIGTRRSPLAIAQAEEVGAHLSEHGMEVELVPMTTSGDEGAAA
                     TAGPQGLKGLWIDTILDALESGEIDLAVHSAKDLPAEEDDGFMIAAVPLRADPGDVLI
                     CRNPGELAHGAVIGTSSLRRRAQVMAAFPRFDVADIRGNVGTRLQKLADREVDALVLA
                     AAGLARLGLNPPHTRPLPLEAMVPAPGQGCLAVQTRDDDEETIGVLRTLDDPVSHRAL
                     DAERSLMWRLGGGCSLPLGAYATVDEDLIRLVAIIATPDGDRVLRVSTEGATPEDAAA
                     EAAKSLIAQGAERILAQVEVG"
     gene            complement(8349..9623)
                     /gene="hemA"
                     /locus_tag="VIX62_13575"
     CDS             complement(8349..9623)
                     /gene="hemA"
                     /locus_tag="VIX62_13575"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY5861305.1"
                     /translation="MAILTLGISFRRAPIELLERLAFTDDDLTKAYRRAHDLDGVDGA
                     VILSTCNRVEVYGDVANYHAGFLALKRLLVETRHVAPEELGEPLYSHWEQDAADHLFA
                     VASGLDSMVLGETQIYAQVREALRRADGEDAADPALTGLFHAAVRAGRRARQETSLGA
                     APDAFVTLGADLASESLGGLEDRDVVVVGAGQMAALAVKHLRHRGVGPVRILNRSLEH
                     ARALAERTNARHGDLDALPDALVTADLVVSATGAAGTVISRDAAATAMRARSGRPLVL
                     LDLAVPRDIDPTIATLEGAEVIDVVAVRDHLAERDEHTAEDIVRAHEIVAEEVRRYVV
                     RRRGDALAPLIKALRRRGDDVVQGELDRQASHLRDLTPDERAAVEALARGIAAKLLHD
                     PIVKLKERSEPGTEGVHARVLAELLGIDPDAT"
     gene            9698..10795
                     /locus_tag="VIX62_13580"
     CDS             9698..10795
                     /locus_tag="VIX62_13580"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY5861306.1"
                     /translation="MDPLQGRARDLGIRIGTGEAGALDAITDVPGVRVGHRTLIRGAD
                     GAPDAVRTGVTAIFPHEGEPWQEWVYAGTHILNGYGELIGINQVTEWGALMSPIVLTS
                     SLQIGKAYDATVRWIAGRDRTGAGEVMPVVSECDDSWLSDVLSFPLSDDDVAAALSDA
                     VSGPVAEGCVGAGTGMACFDFKGGIGTASRRLPADAGGFTVGVLVLTNYGDREFLQID
                     GVHVGEAITDLMPTHHQDGSCVVVVATDAPLLPHQLRRVAQRGGMGLAATGSYASNGS
                     GEQMIAFSTANRLSYGGAVTDVRAVADGPGEEPWLLSRVFEATVEATQEAVVNALLAA
                     ETTTGKDGWTLHAMPVERTLELMAAAGRLHR"
     gene            complement(10818..>11040)
                     /locus_tag="VIX62_13585"
     CDS             complement(10818..>11040)
                     /locus_tag="VIX62_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5861307.1"
                     /translation="RAKLQSSWWGLRPLSPDERPFVGGVREGLMVATGHGSEGVILGS
                     GTAQLVASQIVGEAPPFDPAPFDPLRFEA"
BASE COUNT         1650 a         3856 c         3899 g         1635 t
ORIGIN      
        1 gcaacatctt ccacggcgag ctcaccgtgg atcagctgtt ccacatgcgc ccagccgtgg
       61 gctacgcgga ctaccggtcg cccatcgccg ggctgtacca ggcctcgagc gcgacccacg
      121 caggcggcgg cgtgaccggc cttccgggcc accacgccgt acgcgagatc ttgcaggaca
      181 agaagaagcg ctcaaagcag aagaaaggtc gacggacatg actgccaacc gctacgacgc
      241 gatcgtgatc ggcgggggcc acaacggcct catcaccgcc gcctatctgg ccaagcacgg
      301 tgcccgcacc gtggtgctgg agtcccggca caagaccggt ggcgccgccg acacgatgag
      361 cccgtgggag gaatcgcccg acatcaaggt gaccacgctc agctacgtga tgagcctgat
      421 gccgcccacc atccagaagg atctgcagct cgagcggttc ggctacaagg tgtacccgca
      481 gggcatgggc tacctcccgg tgcccagcgg cggctcgatc atccagcacg acgacgagga
      541 gaagacccgc gagtccatcc gtcagttctc gaagaaggac gccgacgcct acttcgagtt
      601 cgaggcctgg atcggacgca tcgccgacat catggggccg ctgctgatgg agaccccgcc
      661 gaacctgggc tccaagaagg ccaaggacat caaggacgtc gcccaactcg gctggacgct
      721 gcgcaaggag atcgaccccc agacggtggc cgacatcaca cgcctgttca cgatgagcgc
      781 caccgacctg ctggaccgct ggttcgagtc gccggtgctg aagggcttca tggcggtcaa
      841 cggcatcatc ggcacgtggg ccggccccga cgcgcccggc accgcgtacg tgctgatgca
      901 tcactcggtg ggcgacatcg gcgacggcca gatcgcctca tggggctatc ccgaaggcgg
      961 catgggcgcg gtgagtgccg cgtgcgagca ggcggcgcgg ttcttcggcg ccgaggtccg
     1021 cgtgaacgcc cgcgtctcga cgatcaagac cgagaacggc cgggctcgcg gcgtggtgtt
     1081 ggacgggggc gaggagctga tcgcgccgat cgtcgtcacg acgtgtcatc ccaagatcac
     1141 cttcctggac cagctgaacg ccaccgagtt gccggccgag ttcgtgaacg acatcaccca
     1201 ctggaagacg cgcagcggca cggtgaagat caacctggcg ctcgccgagc tgcccgagtt
     1261 cacggccgat cccgggttcg acccgaacgt gcacggcggc gccatccaga tcctggacga
     1321 cgtcgactac ctggagaagg cgttccagga ggctcgattc ggccaggccg ccacgctgcc
     1381 gttcagcgat accgcgatcc cgacggtgtt cgacaagacg ctggcgcccg agggcacgca
     1441 cgtgatgagc atgttcacgc agtgggtgcc gcaggagtgg gccgaggagg atggacacga
     1501 agcggacctc gaggcctacg cggatcgcct gatcgaccgg gtgaacgagg tggcgcccaa
     1561 cttcaaggga tcgatcaaac accgccagat catcggcccc aagcagatgc aggacgagtg
     1621 gggcttgatc ggcggcaaca tcttccacgg cgagctcacc gtggatcagc tgttccacat
     1681 gcgtcccgcg cccggttacg ccgactatcg ctcgccgatc accggcctgt atcaagcctc
     1741 gagcgcgacc cacgccggcg gcggcgtgac cggcatgccc ggccaccact gcgtccgcga
     1801 gatcatgaag gacaaagcgc tcaagaagct caagccgtag cgcaaaggaa ctgtggctcc
     1861 tgttccccgt gggatagtcc tctgccatga gtagggacga gggcttgcgg gccgtcgacg
     1921 aggcgttcga gcgcctgttc gctgacggca cgatccccgg gatggcctac ggggtcgtca
     1981 tcgccggcga cctcgtccat gcgcgcggcc tcggcacgct ccaccagggt gaggaccgcg
     2041 tccccgatgc cgacagcgtc ttccgcatcg cgtcgatgac caagagcttc acggcctcgg
     2101 cggtgctgct cctccgtgac gagggcgcgc tgctcctcga cgaacccgtc gggacctacg
     2161 tgcccgagct cgccgggttc aggggcgcga ccgccgactc gccgttgatc accgtccggc
     2221 atctgctcac gatgtcgagc ggcctgccca ccgacgatcc gtggggcgac cgtctgcagg
     2281 gactcccgct ggatgcgttc gccgagctgc tgggcggcgg attcacgttc gcgtggccgc
     2341 ccgggaccgc gttcgagtac tcgaacctcg gctacgggat cctgggtcgc gtgctcacga
     2401 acgtgagcgg ccaggagtat cgcgacctga tccgcgatcg gttgctcacc cccctcggga
     2461 tggattccac ggtctacgag gccgatcagg ttcccgatgc gcggctcgcc cggggacacg
     2521 tgcaccggga tggcgccttc gtcgacgagc cgttcgatcc ctacggtgca ctggcgtcga
     2581 tgggcggcgt gttcagctcc gtgcgcgacc tcacgacatg ggtgcacggg ttcgccgagg
     2641 cgttccccgc ccgcgacgac cccgagggaa cgcatccgtt gtcgcgtgca tcacgaaggg
     2701 agatgcagca ggtgcagcgc gcattcgatc ccgagctcgg gtggacgtcg gccggagccg
     2761 cgccctcgct cctggccggc ggctacgggt tcgggttgtt cgtctcccag gacctgacgc
     2821 tcggacacgt ggtggatcac agcggtggct acccggggtt cggctcgcac atgcgctggc
     2881 atcccgcgtc gggcgtcggg gtgatcgccc tgggcaaccg cacttacgcg ccgatgtccc
     2941 ggccggcgac cgaagcgctg aaggcgctcg tcgcggcgga ggtcgcggcc gtgggcaccg
     3001 tggtgggctg ggatgccacc gaagccgccc gcgccgatgt cgaacggctg ctcgccgggt
     3061 gggacgaggc cctcgccgct cggcgcttcg cgttcaacgt cgacctcgac gagccgctcg
     3121 ctctccgaag gacctcgttc gaggagatcc gggagcgcca cggagcactc actcgcgatc
     3181 ccgacgagtc ggcgacgagg gactccgcgg cgcacctcgc ctggtggctc acgggcgaac
     3241 gcggcgggcg ggttcgggtg gagatcctga tgaccgggga gccggcgcct cgtgtccaat
     3301 cgatggatgt ccgttcggtc gccgaaccct ccgaggcgtt gcagaccctc gtcgaacgcg
     3361 tggtcgaggt gctgaaccat cagccccccg agccgtcgtg gccgcccgag ctggtgccgg
     3421 ccggtcaaac cgacgccgac cgcgatctcg atggtcgttc gctccgggtc gctgccgcgg
     3481 cgttcgcccc ggtccgccgt atgcgtccga cgcgtggcga tggcaacacc accgtcacgg
     3541 tgccgctgtc tggagctcag ggccgcctcg aactgacgat cgcggtggac gattcgggag
     3601 cgttgaccgc gcgcaccctg cggcccgagc ggctgccggc tggccgaggg tcgtagctcc
     3661 cgctacgtag tgccccggtc ttcatcgaag ggctgcaggt gatctggcgg tgcccctcgg
     3721 cggttggctg cctaggtcga aggtctcgaa cggcatccgc cctgtgtcca tcccccggac
     3781 cggttcccag ataccgacca aaagcctatg cggaaacccg ttcgattacc cgaaatcagg
     3841 tatcagaacg tccccgaagc ccgggagggg tgagcatcat gcgacagtgg gaattgatcg
     3901 gtatcaccga cacggtgccg acgccgccac cgccgccgcg ctcgcgattc gcgaatgccg
     3961 cgacgatcgg cgcggcggcc gaggggcggc ccgtgttctt ctggtccacc gacaccgcgc
     4021 tgcgcctgcg gaccgtcaca ggcgctgcca ccgaggtgat cggcatgccg ttctccaaga
     4081 ccgagggacg cgacctgatc gcggtcttcg ggatggaggg gccgcacctg gcactgctcg
     4141 acgcgcacgt cgccgcgctc aacggcgaga cggccgagtt cacgctcgaa ggtcagcgtg
     4201 ccacggttcg ctgccaggtg gcgccgacgc acgacgccct ggagcgcgtg accggcacct
     4261 tctgcctcgc cgtcgacgtg gagaaggtgg atctgcgcga agtggtggaa cacgcggcgg
     4321 tcgcctagac gaccgctgca cgagaccggg gtaggcccag cccgcagggc ctaacccctg
     4381 aacgacgaca cggccccgag gatcctctcc gtctcctcgg ggccgtgcgt cgtgctgagg
     4441 gtccacagct cgtagcccga cggcggcagg tacacgccgg catccagcag atggtggaac
     4501 agccgggcgt agcgttcgtg atcggcggcg ctcgcatccg agaagttccg gatcggccca
     4561 ccggcgaaga acacgctgaa catcgagccg gcgcggttga tggtcacggg gatatcgatc
     4621 gctgcgaagg cagccgcgat cccgtcggtg aggacgtcgg ccgtcgcttc cagcccggga
     4681 tacggatcgg tggtgcgggc caggtcgagc gcggcgatcc cagccgccac cgccaccggg
     4741 ttcccgctca gtgtgcccgc ctggtagacc ggtccggtgg gcgccagtcg ctccatgagc
     4801 gtcgcgggcc cgccgaaggc cgcgcacggg aacccgccgc ccatcacctt cccgagcgcc
     4861 gtgaggtcgg gcgtcacgcc gtagaaggac tgcgcgccgc cgtaggcgat ccggaacccg
     4921 gtgatcacct cgtcgaacag cagcagcgcg ccgtgctcgg tgcacaactc gcgcaaccct
     4981 tcgaggaatc ccggcgcagg cggcaccacc cccatgttcg ccgcaacggg ttcgacggcc
     5041 accacggcca cctcgccacc acgctcggcg aagatggccc gtacggcctc gagatcgttg
     5101 aacggggcgg tgagcgtgtc cttcgcgctg ccttcggtga cccccggcga cgacgggatg
     5161 cccagggtgg cgagtccgct gcccgcgccc ttggccagga gcgagtccga gtgcccgtgg
     5221 tagcagcctt cgaacttgac caccaggtcg cggcccgtgg cgccgcgcgc gagccggatc
     5281 gcgctcatcg ttgcctcggt gccgctgttc accaaccgca cttgctccac gccgggcacc
     5341 gcgtcgacga tgcgctgcgc gagttccacc tcgccctcgg tgggcgtgcc gaacgaggtc
     5401 cccttcgcgg cggcgcgcag cgccgcttcg acgatctcgg gccgggcatg gccgaacagc
     5461 aacgctcccc acgactgcac gagatcgacg tagcggttgc cgtcgacgtc gatgaggaac
     5521 gcgccctcac cgcggtcgaa gaacaccggc gtgccgccca cgcccccgaa ggcccgaacc
     5581 ggagaatcga ccccgcccgg gatcacggtg cgagcgcggg cgaacaggtc ctcggatctg
     5641 gtcatgccgt ggatggctca ggggcggtcg gacgcgagcc agccggcggc gtccttcgca
     5701 tggtaggtga ggatcaggtc ggcgcccgca cggcggatcg acgtgagggt ctcgagcacg
     5761 ctgcgtcgtt cgtcgagcca gccgttggcg gccgcggcct tcagcatcga gtactcgccg
     5821 ctgacgtggt aggcggcgag cggcacggcg gtctcctgct tggcccgcga cacgatgtcg
     5881 aggtagggca cggccggctt caccatcacg atgtcggcgc cttcgtcgat gtcggtgcgg
     5941 atctcgcgga gcgcttcatc ggcgttggcg ggatcctgct ggtagcccgt gcgatcgccg
     6001 aagctcgggg cgcactcggc cgcctcgcgg aacggtccgt agaaggcgct cgcgaacttc
     6061 gccgcgtacg cgatgatccc gacgtcggtg tgggcggcgc cgtccaacgc gtcccggatc
     6121 gcggccacct gaccgtccat catgccgctg ggccccacca gatgcgttcc ggcctccgct
     6181 tgcgcgatcg cgatgcgccg gtaccgctcc acggtctcgt cgttgtcgac gtcggtgccg
     6241 tcggcggtca ggaccccgca gtggccgtga tcggtgtatt cgtccaggca cagatcgctc
     6301 atcaccacgg cgtcgtcgcc gaactcggtg cggagggcgg cgatcgcctg ctggccgatg
     6361 cccgacgggt tccaggcctc ggacccctcg gcgtccttgc gcgccggcac cccgaacagg
     6421 atgaacgcgc gaacgccggt ctcggcgatc tcgaccgcct ccttgcgcag cgattcgagc
     6481 gtgtgctgga actggccggg catggaggcg atggcgaccg gctcgtcgat gccttccttc
     6541 gcgaagagcg gcgcgatcag atcgccggcg tcgagcgagg tctctcggat cagcgctcgg
     6601 agcgcgggtg tccgccggag ccgccgggga cgagacgcgg ggtagctcat gacctcaggg
     6661 taccggggcg ggcccgtcag gagccagcgc ggcggcgagg ggtgaagacg cgctcgagcg
     6721 ccgcgaccac gccgtcgatc gtgtggggat tcgcaacggc ggatacggtc agcccgtggg
     6781 ctcgcgcctc gttcgccgtc accggtccga tgcacaccac cctggggttg cccttcaccg
     6841 ggccgagtgc cccgacgaac ccgcgcacgg tggaggcgct ggtgaacgtc acggcgtcga
     6901 cggttcctgc gcgcagggcc tcgcgtgctt ccgcgggcag ggtgcgtggc atccgcgtgc
     6961 ggtaggcgtc cacgcgcgtg gggcgccagc ccttggaggc caacgcatcc tccagcccct
     7021 ccggcgcgat gtcggcgcgt gcacacagca cccgcccctc gccccggggg aacgcccgcg
     7081 ccaagccaac cgtggtgaag gtcggcggca ccaggtcggg cgcgcgtccc gcccaacgac
     7141 ggaaggcttc cgccgagccg tcaccgatca cggcgacctt cgtgcggacg tccgcggggg
     7201 agcccagcct cgatgcgagc atctccacgg tggcccggct cgtgatggtg atccacgaga
     7261 agtcaccgtc cgcgaggtcg cgaagggcgg tggtcagggc cgccgatcgg atcggccgta
     7321 cctcgatggc gggggcgacg atcgtgtgtg cgctacgttg gtcaaggagc ttgaccaatg
     7381 acgctgactg atccgcgggc cgggtgagga ggatcgtgcg gcccgcgagc ggcttcggcg
     7441 agcgcgggct cacccgacct cgacctgcgc caggatccgt tcggcgcctt gggcgatcag
     7501 cgacttcgcg gcctcggcgg cggcatcctc tggggtcgcg ccctcggtgc tcacgcgcag
     7561 gacacggtcg ccatcggggg tggcgatgat cgccaccagc cggatcagat cctcgtccac
     7621 ggtggcgtac gccccgagcg ggagcgaaca gcccccgccc agccgccaca tcagggaccg
     7681 ctcggcgtcc agggctcggt ggctcaccgg atcgtccagg gtccgaagca cgccgatcgt
     7741 ctcctcgtcg tcgtcacggg tctgcaccgc gaggcaaccc tggccgggcg ccgggaccat
     7801 cgcctcgagc ggcagcggcc gggtgtgggg agggttcagt cccagccgcg ccagccccgc
     7861 cgcggccagc accagggcgt ccacctcgcg atcggccagc ttctgcagcc gcgtgccgac
     7921 gttgccgcgg atgtcggcca catcgaaccg tgggaacgcc gccatcacct gcgcgcgccg
     7981 gcgcaggctg gacgtgccga tcacggcgcc gtgcgcgagc tcgccggggt tgcggcagat
     8041 caggacgtcg ccgggatcgg cgcggagcgg cacggcggcg atcatgaacc cgtcgtcctc
     8101 ctcggcgggc aggtccttcg ctgagtgcac cgccaggtcg atctctccgg attccagcgc
     8161 gtcgaggatc gtgtcgatcc acagaccctt gaggccctgc ggccccgccg tggcggccgc
     8221 accttcgtcg ccggaggtgg tcatgggcac gagctcgacc tccatgccgt gctccgacag
     8281 gtgggcgccg acctcttcgg cttgggcgat cgcgagcggc gagcggcgcg tgccgatccg
     8341 catggggctc acgtcgcgtc ggggtcgatc cccagcagtt ccgccaggac gcgggcgtgg
     8401 acgccctcgg tgccgggttc ggagcgttcc ttcagtttca cgatgggatc gtgcagcagc
     8461 ttcgcggcga tcccccgggc gagcgcctcg accgcagccc gttcgtcggg cgtgagatcg
     8521 cgcaggtggg atgcctggcg gtcgagttcg ccctggacga cgtcgtcgcc gcggcggcgg
     8581 agggccttga tcaagggagc gagcgcgtcg ccgcggcggc gcacgacgta ccggcggacc
     8641 tcctcggcca cgatctcgtg cgcgcgcacg atgtcctcgg cggtgtgctc gtcgcgctcg
     8701 gcgaggtggt cgcgcaccgc cacgacgtcg atcacctcgg cgccctcgag cgtggcgatc
     8761 gtgggatcga tgtcgcgcgg caccgcgaga tccagcagga ccaggggacg cccgcttcgc
     8821 gcccgcatcg ccgtcgccgc cgcgtcccgc gagatgaccg tgccggcggc gccggtggcg
     8881 ctcacgacca ggtcggccgt gaccaacgcg tcggggagcg cgtcgaggtc gccgtgccgg
     8941 gcgttcgtgc gctcggccag ggcccgcgcg tgctccaggg aacggttgag gatgcggacc
     9001 ggccccacgc cccgatggcg gaggtgcttc acggccaggg ccgccatctg cccggcgccc
     9061 acgaccacga catcgcgatc ctcgagcccg ccgagcgatt cgctggcgag atcggcgccc
     9121 agcgtcacga aggcgtcggg ggcggcgccg agcgaggttt cctgacgagc gcgacgtccg
     9181 gcgcggacgg cggcatggaa cagcccggtg agggcggggt cggcggcatc ttcgccgtcg
     9241 gcacgccgaa gcgcttcccg cacctgggca tagatctgcg tctcgcccag caccatcgag
     9301 tcgagtccgc tcgccaccgc gaacagatgg tcggcggcgt cctgctccca gtgggagtac
     9361 aggggttcgc ccagttcctc gggagcgacg tgccgggtct cgaccagcag gcgcttcagg
     9421 gccaggaacc cggcgtggta gttggcgacg tcgccgtaga cctcgacccg gttacaggtg
     9481 gacaggatca cggcgccgtc gacaccgtcc agatcgtggg cgcgacggta cgccttggtg
     9541 aggtcgtcgt cggtgaacgc gagccgctcc agcagctcga tcggtgcgcg acggaaggag
     9601 atcccgaggg tgaggatggc catgggcggt aggtcaggga ttctagccgc ctgaggctcc
     9661 ctaccgccca ccgacctggc aggtagcatc cgccgcgatg gatcccctcc agggccgtgc
     9721 ccgtgacctc ggcatccgga tcggcaccgg tgaagccggt gccctcgacg ccatcaccga
     9781 tgtgcccggc gtccgcgtgg gccatcggac gctgatccgt ggggccgacg gcgctcccga
     9841 tgcggtccga accggtgtca ccgcgatctt cccgcacgag ggtgagccct ggcaggagtg
     9901 ggtctacgca ggcacgcaca tcctgaacgg gtacggcgag ctgatcggca tcaaccaagt
     9961 caccgagtgg ggcgcgctga tgagtccgat cgtgctgacc tcgtcgctgc agatcggcaa
    10021 ggcctacgac gccaccgttc gctggatcgc cggccgcgac cggacgggcg ccggggaggt
    10081 catgcccgtg gtcagcgagt gcgacgactc gtggctgtcc gacgtgttgt ccttcccgtt
    10141 gtccgacgac gacgtcgcgg cggcgctctc cgacgccgtc tccgggccag tggccgaggg
    10201 gtgcgtcggc gccggcaccg gcatggcgtg cttcgacttc aagggtggga tcggcaccgc
    10261 gtcgcggcga ctgccggccg acgccggtgg gttcaccgtg ggggtgctcg tcctcacgaa
    10321 ctacggcgac cgtgagttcc tgcagatcga cggcgtccac gtcggtgagg cgatcaccga
    10381 tctgatgccg acgcatcatc aggacggttc gtgcgtcgtc gtggtcgcca ccgacgcacc
    10441 gctgctgccc caccagctcc ggcgggtggc ccagcgcggc ggcatggggc tcgccgccac
    10501 cggctcgtac gcctccaatg ggagcggcga gcagatgatc gcgttctcga ccgcgaaccg
    10561 cctgtcctac ggtggcgccg tcaccgacgt gcgcgcggtg gccgacggcc ccggcgagga
    10621 gccgtggctc ctgtcccgcg tgttcgaggc gaccgtcgag gccacccagg aggcggtggt
    10681 caacgcgttg ctggcggccg agaccaccac gggcaaggac gggtggaccc tgcacgccat
    10741 gccggtcgaa cgcacgctcg agctgatggc cgccgccggc cgcctgcacc gctaattgtc
    10801 tctggttcgc tccctggtca cgcctcgaag cgcagcgggt cgaagggtgc agggtcgaac
    10861 ggcggcgctt cgcccacgat ctgtgaggcc accagctggg ccgtgccgct cccaaggatc
    10921 acgccttcgg agccgtggcc ggtagccacc atcaagccct cgcggacgcc gccgacgaac
    10981 ggtcgctcgt cgggcgagag cggtcgcagg ccccaccagg atgactgcag cttcgcgcgt
//
LOCUS       DATDBZ010000587         2334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2658939, whole genome shotgun sequence.
ACCESSION   DATDBZ010000587 DATDBZ010000000
VERSION     DATDBZ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658939"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VIX62_13590"
     CDS             complement(<1..283)
                     /locus_tag="VIX62_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5861308.1"
                     /translation="MNVPDKPSLDGLEAKWDEWWEASGTYRFDPTKSREQIYAIDTPP
                     PTVSGSLHVGHVMSYTHTDLMARFKRMRGFEVFYPMGWDDNGLPTERRVQ"
     gene            478..>2334
                     /locus_tag="VIX62_13595"
     CDS             478..>2334
                     /locus_tag="VIX62_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5861309.1"
                     /translation="MQVPVEERRVVSVLFADVTGSTTLGERMDPEQLRDVMATFFGGM
                     REEIEAEGGTVEKFIGDAVMAAFGVPSAHEDDPARALRAAIRMRRRLEEVNTELDATH
                     GLTLQMRIGVNTGEVLAAGDAPSGEPMVTGDVVNTAARLQTIAGPGEILAADRTRRAV
                     RGFRFGERQDLALKGRSESVVASAVLGGSGEAADVGLSGPMVGRDAELGLLRSMYERS
                     AAERRPHVVTIYGDPGVGKSRLTHEFIGWAASQERAPLVVRGRCLPYGDGITYWPLSE
                     ILRSIADVSDTDTPSVALDKIRSVGMTLLRTAPDPERATAAIAFTTGLEDPDTPLFDE
                     EPKRVRDEMHAAWRTFFSALAADRPVVVVVEDIHWADPAMLDLLEELADRVEGGVSFV
                     CPSRPDLTSTRPGWGGGKRNYSAIALDPLGTRDADTLVRSLLDVDGLPDSMFRQILDR
                     AEGNPFFLEEIVRQLVDSRSVWLEGGRWRAAGDITQVTIPDTVQGVLAARIDLLDPPD
                     RRVLQAAAVVGRIFWPGAVRQLLNGAGDRVPEALITLEERDLVRSRLSSALGDEPEYI
                     FKHVLTRDVAYDTLPRRDRSAAHASIARWLEDTLGERAREFLELLSYHWFEAY"
BASE COUNT          339 a          811 c          809 g          375 t
ORIGIN      
        1 tctggacgcg gcgctctgtg ggcaggccgt tgtcgtccca gcccatcggg tagaagacct
       61 cgaacccacg catgcgcttg aaccgcgcca tgaggtcggt gtgcgtgtag ctcatcacat
      121 ggcccacgtg cagcgagccg ctcaccgtgg gcggcggcgt gtcgatcgcg taaatctgct
      181 cgcgtgactt cgtggggtcg aaccggtagg tgccggaggc ctcccaccat tcgtcccact
      241 tggcttccag tccgtcgagc gacggcttgt cgggcacgtt catggcggca gtctacggaa
      301 cggccctaga ctcggccgat ggttcgctgc gccacttgtg gttccgagaa cgacgccgag
      361 gctcggttct gctcgacgtg cggcacgggc ctgcgtccgg tgtgcgcctc ctgcggggtg
      421 gagctgccgc ccgacgctcg cttctgcccc gcttgcggaa ccccggtcga ggaaacggtg
      481 caggtcccgg tcgaggaacg acgggtcgtc agcgtgttgt tcgccgacgt caccgggtcg
      541 acgacgctgg gtgaacgcat ggaccccgag cagctgcgcg acgtcatggc caccttcttc
      601 ggtggcatgc gcgaggagat cgaggccgaa ggcggcacgg tggagaagtt catcggcgac
      661 gccgtgatgg ccgcgttcgg cgtgccgtcg gcccacgagg acgatcccgc gagggcgctc
      721 cgagccgcga tccgcatgcg gcgacggctc gaggaggtca ataccgaact cgacgccacc
      781 catgggctca cgttgcagat gcggatcggg gtcaacaccg gcgaggtcct cgcggccggg
      841 gacgcgccat cgggcgagcc catggtgacc ggtgacgtcg tcaacaccgc ggcccgcctg
      901 cagaccatcg ccggacccgg cgagatcctg gccgcggacc gaacccgccg cgcggttcgc
      961 ggattccgct tcggcgagcg ccaggacctc gcgctcaagg gcaggtccga gtcggtcgtg
     1021 gcctcggccg tgctcggcgg atccggtgag gccgccgacg tgggtctcag cggcccgatg
     1081 gtgggccgcg atgccgaact cggactgctc cggtccatgt acgagcgctc ggccgccgag
     1141 cgccgaccgc acgtcgtcac gatctacggc gacccggggg tgggcaagag ccggctgacc
     1201 cacgagttca tcgggtgggc cgcctcgcag gaacgagctc cgttggtcgt gcgggggcga
     1261 tgtctcccgt acggcgacgg catcacgtac tggcccttgt ccgagatcct gcgaagcatc
     1321 gcggacgtct cggacaccga caccccctcc gtcgcactcg acaagatccg ctccgtcggg
     1381 atgacgctcc tgaggaccgc tcccgacccc gaacgcgcca ccgccgccat cgccttcacg
     1441 acgggcctcg aggaccccga cacgccgctg ttcgacgagg agccgaaacg ggtgcgcgac
     1501 gagatgcacg cggcgtggcg caccttcttc tcggcgctgg cggcggaccg gcccgtcgtc
     1561 gtggtggtgg aggacatcca ctgggcggat ccggccatgc tggacctgct cgaagagctc
     1621 gccgaccggg tggagggcgg cgtgtccttc gtgtgtccct cgcgcccgga cctcaccagt
     1681 acccgccccg gatggggagg aggcaagcgc aactattccg ccatcgccct cgatcccctg
     1741 ggcacgcgag atgccgacac gttggtccgc tcgttgctcg acgtcgacgg gctccctgac
     1801 tcgatgttcc gccagatcct cgaccgtgcg gagggcaacc cgttcttcct ggaagagatc
     1861 gtgcggcaac tggtggacag ccgttcggtc tggctcgaag gcgggcggtg gcgcgccgcc
     1921 ggtgacatca cgcaggtgac gatccccgac acggtgcagg gggtcctagc cgctcgcatc
     1981 gacctgctcg acccgccgga tcgtcgggtg ctgcaggcag cggccgtcgt cggacgcatc
     2041 ttctggccgg gcgcggtccg gcagctgctg aacggcgcag gcgatcgcgt gcccgaggca
     2101 ctcatcaccc tcgaggagcg cgacctcgtg cggtcccgac tctcctcggc tctgggcgat
     2161 gagcccgagt acatcttcaa acacgtgctc acacgcgacg tcgcctacga cacccttccc
     2221 cgacgggatc ggtcggcggc gcacgcctcg atcgcccggt ggctcgagga caccttgggc
     2281 gagcgcgctc gcgagttcct cgagttgctc tcctatcact ggttcgaggc ctac
//
LOCUS       DATDBZ010000588         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2850704, whole genome shotgun sequence.
ACCESSION   DATDBZ010000588 DATDBZ010000000
VERSION     DATDBZ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2850704"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VIX62_13600"
     CDS             <1..558
                     /locus_tag="VIX62_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY5861310.1"
                     /translation="YPILLHEADASGPGANGIIMLPYFAGERTPILDPDARGVIAGLT
                     LGHTRGDLYRAALEATAFGVRHNVETMRAAGADIRRIVAVGGGTQGRLWLQIVSDVTG
                     LVQEVPQTTIGASYGAAFLAATAVAPPDEPPRIHEWNPIASTIRPDAERAVLYEDLFA
                     LYLELYAGTTSVVHDLAARQRRHHP"
     gene            578..>2080
                     /locus_tag="VIX62_13605"
     CDS             578..>2080
                     /locus_tag="VIX62_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HEY5861311.1"
                     /translation="MNTYNIPTPTTRPAAPPKTAYLIASGDLRESANTAGWSTQAQME
                     ADVTRAFNELGWHVVRANDVDPVTGHGFISSQRMGLEVFKGIPSQAPLIVAEAVWQYS
                     HHVLAGLRTHEGPILTVANFAGDWPGLVGLLGLNAGMTKMGTEYSTVWSVDFTDEWFT
                     SGVKEWTETGRITHDASHVRPLPELPATPEVELGRALGEQLLADKAIIGVFDEGCMGM
                     YNAIFDDELLNPLGIYKERLSQSALYAEMLTVTDAEADAARTWLTDRGMTFRFGTDEK
                     TELTEAQVRWQLKMYIAALRIADDFGLDAVGIQYQQGLKDLVAASDLAEGILNSTERP
                     PVLSRDGSRELFAGRAFPHFNEADEGVAVDALITDRVWTAMGLVPDNTLHDVRWGEEY
                     DSEFVWVYEISGSVPASHLGGWDKAEGWRQDPVFFPAGGSTINGVSKPGEVVLSRVFI
                     ADGILQADVFRGTVIELPDEETQRRKDATNPEWPIAHVVLHDISRDQFMAR"
BASE COUNT          359 a          711 c          691 g          319 t
ORIGIN      
        1 tatcccatcc tgcttcacga ggcggatgcc tcgggccccg gcgcaaatgg gatcatcatg
       61 ctgccctact tcgccggcga gcgtacgccg atcctcgacc ccgatgcgcg cggcgtgatc
      121 gcagggctca cactggggca cacacgcggt gacctctatc gtgcagcact ggaggcgacc
      181 gcgttcggcg tgcggcacaa cgtcgagacg atgcgagccg ccggtgccga catccggcgc
      241 atcgtcgcgg tcggcggcgg aacccaggga aggctctggc tgcagatcgt ctccgacgtc
      301 acgggtctcg tgcaggaagt tccgcagacc acgatcggcg cgagctacgg cgcggcgttc
      361 ctcgctgcaa cggcggtggc accgcccgac gaaccgccac gcatccatga gtggaacccg
      421 atcgcgtcga ccatccgccc ggatgccgaa cgtgcggtgc tctacgagga tctgttcgcc
      481 ctctacctcg agctctacgc gggaacgaca tccgtcgtcc acgacctcgc cgcccgacag
      541 cggcgccacc acccctgacg ccacggaagg atctcatatg aacacctaca acatccccac
      601 cccgaccacg cgccccgctg cgccgccgaa gacggcgtac ctgatcgcga gcggcgatct
      661 gcgggagtcg gcgaacaccg caggctggtc gacgcaggcg cagatggagg ccgacgtcac
      721 tcgggcgttc aatgagctgg gctggcacgt cgtgcgtgcc aacgacgtcg atcccgtcac
      781 cgggcatggc ttcatctcca gtcagcgaat gggtctcgag gtgttcaagg gcatccccag
      841 ccaggcgccg ctgatcgtcg ccgaggcggt gtggcagtac tcgcaccacg tgctcgccgg
      901 gctgcgcacg catgaggggc ccattctcac cgtggcgaac ttcgcgggcg actggccggg
      961 gctggtcggc cttctcgggc tcaacgccgg catgaccaag atgggcacgg agtactcgac
     1021 ggtctggtcg gtcgacttca ccgacgagtg gttcacgagc ggagtgaagg agtggaccga
     1081 gacgggccgc atcacgcatg acgcctcgca cgtacgtccg ctgcccgaac tgcccgccac
     1141 tcccgaagtc gagctcggcc gcgcgctcgg cgaacaactg ctcgccgaca aagccatcat
     1201 cggcgtgttc gacgagggct gcatgggcat gtacaacgcg atcttcgacg acgagctgct
     1261 caacccgctc ggcatctaca aggagcgcct gtcgcagtcg gcgctgtacg ccgagatgct
     1321 gacggtgacg gatgcggagg ccgacgccgc ccgcacatgg ctcaccgacc gcggtatgac
     1381 gttccggttc ggcaccgatg agaagaccga gctgaccgag gcgcaggtgc ggtggcagct
     1441 gaagatgtac atcgccgcgc tgcggatcgc ggacgacttc gggctcgatg cagtcggcat
     1501 ccagtaccag cagggcctga aggacctggt cgcggcatcc gatctcgccg aaggcatcct
     1561 caactcgacc gagcggcctc ccgtgctctc acgcgacggc tcgcgcgagc tgttcgccgg
     1621 ccgcgccttc ccccacttca acgaggccga cgagggcgtc gccgtcgacg cgctgatcac
     1681 cgaccgggtg tggaccgcga tgggcctggt gcccgacaac accctccacg acgtgcgctg
     1741 gggcgaggag tacgacagcg agttcgtgtg ggtgtacgag atctcgggct cggtcccggc
     1801 ctcacatctg ggcggttggg acaaggcgga ggggtggcgc caggatccgg tattcttccc
     1861 ggccggcggg tcgacgatca atggagtgtc gaagcccggc gaggtcgtgc tctcacgcgt
     1921 gttcatcgcg gacggcatcc tgcaggccga cgtcttccgc ggcaccgtga tcgagctgcc
     1981 cgatgaggag acgcagcgac gcaaggatgc cacgaacccc gagtggccga tcgctcacgt
     2041 cgtgctgcac gacatcagcc gcgatcagtt catggccagg
//
LOCUS       DATDBZ010000589         3882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_403013, whole genome shotgun sequence.
ACCESSION   DATDBZ010000589 DATDBZ010000000
VERSION     DATDBZ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403013"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VIX62_13610"
     CDS             <1..501
                     /locus_tag="VIX62_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5861312.1"
                     /translation="LPILDGTCSLHHQASFYETFWNPEGGDAVSVASDGDVFGFLLPP
                     ASADDPLSVTGGGEIVVAFREADEVEAFRAFLSSDTWANNRVSLGGVISANKGVDPEN
                     ASSELLVQSIEILQDPETTFRFDGSDLMPGAVGADSFWKGIVAWVGGESTTQVLDTIE
                     SSWPSS"
     gene            614..1582
                     /locus_tag="VIX62_13615"
     CDS             614..1582
                     /locus_tag="VIX62_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015489572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY5861313.1"
                     /translation="MTTADLLGKILQVVMGLAVFAAVVGLLIFLIDKAPKKGRDYWQL
                     AGFLAPAMILVAIGLIYPAIRTSILAFQDSSGDWTLDNFVWTFTQPAAIRTLINTVIW
                     VLLVPTFATAVGLAYAVFIDKSRGERYYKAILFMPIAISFVGASVIWKFVYEYRSGER
                     EQIGLLNAIVTAFGGEPVQWLQTDPINTILLIVVMIWVQTGFAMVVLSAAIKGIATEQ
                     IEAAQLDGTNAWQRFTNVTIPGIRGALVVVITTISIATLKVFDIVRTMTAGNFNTSVV
                     ANEMYTQAFRASEIGRGSALALILLVLVLPIIIYNVNVLRKQREIR"
     gene            1579..2571
                     /locus_tag="VIX62_13620"
     CDS             1579..2571
                     /locus_tag="VIX62_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010204062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY5861314.1"
                     /translation="MSSVAPADLPVGKDLGTLEATEESSYAQPKTMRVKKQLTSRTAT
                     IASLIIAVLWTIPTFGLFISSFRPAELIRSTGWWTIFANPGFTLENYRDVLFSSSGSS
                     PQLGAYIVNSIAIALLATIIPLVFASMAAYAFAWMRFRGVGVLFIIIFALQIIPLQMA
                     LVPVLQIFSTVLRPMQAWLHDVIPIIPEQNYLPLWIAHSMFALPLAIFLLHNFISEIP
                     ADVIEAARVDGATHGQIFWRIVLPLAVPAIASFAIFQFIWVWNDLLVALIFSGGTQDV
                     APLTQRLAELTGTRGQDWQLLTAAAFISIVIPLVVFFSLQRYFVRGLLAGSTKG"
     gene            2689..>3882
                     /locus_tag="VIX62_13625"
     CDS             2689..>3882
                     /locus_tag="VIX62_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY5861315.1"
                     /translation="MAASRLVLGPMQRFVDETSASIWVETRADCRVTVRAEGRSWHAR
                     TFAVHGHHYALVEVAGLEPGTTRPYDVEVDGRRAWPEPDSEYPPPVIATRTPGRPVRM
                     AFGSCRTSVSHDRHGNRTHGVDSLRAFALAVANGRAERPDLLLFLGDQVYADSTSKEM
                     QDFIRARRDIHEEPGAELKDYEEYGHLYELAWSDPANRWLLSTVPSAMIFDDHDIRDD
                     WNASLDWKRKMEATTWWHGRIVAGLASYWVYQHLGNLSPRAREADELWRRIIGHDGTG
                     ELDLTEVIDAFAERSDADPDSYGWSYCRDFDDVRLIVVDSRVARDLTPSARGLLDAQE
                     LHWFDERMRGGFRHVLVATSLPFLLPLGLHHVEAWDEAIAQGAWGRPAAWIGERLRQA
                     VDLEHW"
BASE COUNT          605 a         1315 c         1294 g          668 t
ORIGIN      
        1 ctgccgatcc tcgacggaac gtgctcgctg caccaccagg catcgttcta cgagacgttc
       61 tggaaccccg agggcggcga cgccgtttcg gtggcatccg acggcgacgt gttcggcttc
      121 ctgctcccgc cggccagcgc cgacgacccg ctgtcggtca ccggtggcgg cgagatcgtc
      181 gtggccttcc gtgaggccga cgaggtggag gcgttccgtg ccttcctctc gagcgacacc
      241 tgggcgaaca accgtgtcag cctgggcggc gtgatcagcg cgaacaaggg cgtcgacccc
      301 gagaacgcat cgagcgagct gctcgtgcag tcgatcgaga tcctgcagga tcccgagacg
      361 acgttccggt tcgacgggtc cgacctgatg cccggtgccg tcggcgccga ttccttctgg
      421 aagggcatcg tggcctgggt cggcggtgag agcaccacgc aggtgctcga caccatcgag
      481 tcctcctggc cgtccagcta gcactccgcg agtcgcgccc ggcgagcgcc gaggacgcgg
      541 catgatcgag gggcggggtt gaaggacccc gcccctcggc accccagctt caacgacgaa
      601 cccgggaggc acgatgacca cggccgatct cctcggcaag atcctgcagg tggtgatggg
      661 gctcgcggtg ttcgccgcgg tcgtcggcct cctcatcttc ctcatcgaca aggccccgaa
      721 gaagggccgc gactactggc aactggcggg gttcctcgca cccgcgatga tcctggtggc
      781 gatcggcctc atctatccgg ccatccgcac cagcatcctg gccttccagg actcgtcggg
      841 cgactggacc ctcgacaact tcgtctggac gttcacgcag cccgcggcga tccgaacgct
      901 catcaacacg gtcatctggg tgctgctcgt cccgacattc gcgaccgccg tgggcctggc
      961 gtacgccgtg ttcatcgaca agtcccgcgg agagcggtac tacaaggcga tcctgttcat
     1021 gccgatcgcg atctcgttcg tgggtgcgag cgtcatctgg aagttcgtct acgagtaccg
     1081 ctccggcgag cgggagcaga tcggcctcct caacgcgatc gtcaccgcct tcggcggtga
     1141 gccggtgcag tggctgcaga ccgatccgat caacacgatc ctcctcatcg tcgtgatgat
     1201 ctgggtccag accggattcg cgatggtcgt gctgagcgcc gcgatcaagg gcatcgcgac
     1261 cgagcagatc gaggcggcgc agctcgacgg caccaacgca tggcagcgct tcaccaacgt
     1321 caccatcccg ggcatccgcg gggcgctggt ggtcgtgatc acgacgatct cgatcgccac
     1381 cctgaaggtc ttcgacatcg ttcgcacgat gacggccggt aacttcaata caagcgtcgt
     1441 ggccaacgag atgtacaccc aggcgttcag ggcgagcgag atcgggcgcg ggtccgcgct
     1501 cgcgctcatc ctcctcgtcc tcgtgctgcc gatcatcatc tacaacgtca acgtgcttcg
     1561 caagcagagg gagatccgat gagcagcgtc gccccggccg accttcccgt cggcaaggac
     1621 ctcggcaccc tcgaggccac cgaggagtcc tcgtacgcgc agcccaagac gatgcgcgtc
     1681 aagaagcaac tcacctcgcg cacggcgacg atcgcgtcgc tcatcatcgc ggtgctctgg
     1741 acgatcccga ccttcgggct cttcatctcc tcgttccggc ccgcggagct gatccgctcc
     1801 accggatggt ggacgatctt cgcgaatccg gggttcacgc tggagaacta ccgcgacgtg
     1861 ctgttctcgt catcggggtc gtcgccgcag ctcggcgcgt acatcgtgaa ctcgatcgcg
     1921 atcgcactac tggcgacgat catcccgctc gtgttcgcgt cgatggcggc gtacgccttc
     1981 gcctggatgc ggttccgcgg cgtgggcgtg ctgttcatca tcatcttcgc gctgcagatc
     2041 atcccgttgc agatggccct cgtgccggtc ctgcagatct tctcgaccgt gctgcggccg
     2101 atgcaggcct ggctgcacga cgtcatcccg atcatccccg agcagaacta cctgccgctg
     2161 tggatcgcgc actcgatgtt cgcactgccg ctggcgatct tcctgctgca caacttcatc
     2221 tcggagatcc cggccgacgt gatcgaggcg gcgcgggtcg acggagcgac gcacgggcag
     2281 atcttctggc gcatcgtgct gccgctggcg gtgcccgcga tcgcctcgtt cgcgatcttc
     2341 cagttcatct gggtgtggaa cgacctgctc gtcgccctga tcttctcggg cggcacgcag
     2401 gacgtcgcgc cactcaccca gcgattggcg gagctgacgg gtacccgcgg acaggactgg
     2461 cagttgctga cggcggccgc gttcatctcg atcgtgatcc cgctggtcgt cttcttcagc
     2521 ctccagcggt acttcgtgcg cggtcttctg gcgggctcca cgaaggggta ggaacgggcc
     2581 cgccccacgg gcggacgggc agggggaacg gatgccgcga gcgggggctc gcggcatccg
     2641 ccatgctccc catggcactc cgaggcgaga cgcggtagaa cgggatgcat ggcggcctcg
     2701 cgactggtac tcggtccgat gcagcggttc gtcgacgaga catcggcgag catctgggtg
     2761 gagacgcgag ccgattgccg agtgacggtt cgcgccgagg ggcggtcctg gcacgcccgc
     2821 acgttcgccg tgcacggcca ccactacgcc ctcgtggagg tcgccggcct cgagccgggc
     2881 acgacgcggc cctacgacgt ggaggtcgac ggccggcgcg cctggccgga gccggattcg
     2941 gagtacccgc cgccggtgat cgcgacgcgc acgccgggtc ggccggtgcg gatggccttc
     3001 gggtcatgcc gtacgagcgt ctcccatgac cggcacggga atcgcacgca cggcgtggac
     3061 tcgctgcgtg ccttcgccct cgccgtcgcc aacggccgag cggagcgtcc cgatctgctc
     3121 ctcttcctgg gcgaccaggt gtacgcggac tcgacctcga aggagatgca ggatttcatc
     3181 cgcgcccggc gcgacatcca cgaggagccg ggcgcggagc tgaaggacta cgaggagtac
     3241 gggcatctct acgagctcgc atggtcggac cccgccaatc gatggctcct gtcgacggtc
     3301 cccagcgcca tgatcttcga tgaccacgac atccgcgacg actggaatgc ctcactcgac
     3361 tggaagcgga agatggaggc cacgacgtgg tggcacggtc gcatcgtcgc cggcctggcg
     3421 tcgtactggg tgtaccagca cctgggcaac ctgtcgcccc gagcacgcga ggcggacgag
     3481 ctgtggcgtc gcatcatcgg ccacgacggg acgggagagc tcgacctcac cgaggtcatc
     3541 gacgcgttcg ccgagcggag cgatgcggat cccgacagct acggctggag ctactgccgg
     3601 gacttcgacg acgtgcggct gatcgtcgtc gactcgcggg tggccaggga cctgacgccg
     3661 tcggctcgtg gcctgttgga tgcccaggag ctgcactggt tcgatgagcg gatgcgcggt
     3721 gggttccggc acgtcctcgt cgccacctcg ctgccgttcc tgctgcccct gggcctccac
     3781 catgtcgagg catgggatga ggccatcgcc cagggcgcgt gggggcgacc ggcggcgtgg
     3841 atcggggaac ggctgcggca ggcggtcgac ctcgagcact gg
//
LOCUS       DATDBZ010000590         8219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_815676, whole genome shotgun sequence.
ACCESSION   DATDBZ010000590 DATDBZ010000000
VERSION     DATDBZ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815676"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="VIX62_13630"
     CDS             complement(<1..1604)
                     /locus_tag="VIX62_13630"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY5861316.1"
                     /translation="MSDRPANRLAHQTSPYLLQHAHNPVDWYPWGPDALNAARERDVP
                     IFLSIGYAACHWCHVMERESFEDEATAALMNTSFVSIKVDREERPDLDGIYMDAVQAM
                     TGHGGWPMSAFLTPDGRPFYAGTYYPPEPRPGMPSFRQVLTGIAEAWDERRDEIVAQA
                     GKVTESIASVGSLEGSNDPLTGEVAREAMQALRPSFDGRWGGFGGAPKFPQPMTLEFC
                     LRQSLRGVPDALEMVVISLDRMAEGGIYDHVGGGFARYSTDGAWHVPHFEKMLYDNAQ
                     LVQLYTRTWQVTGEDRYRRVATETVEYLLREMQRPEGGFFSSQDADSEGVEGRFFTWA
                     WNELVDLVGEPVAACFGATVEGNWAGEEGRTNVLWRPIATSAVAREHGLDPTDLEAMV
                     EDARTVLFEARDARVHPGTDDKVLTAWNAMVIRALAEAGRAFDEPRYIEAARRCASFV
                     QTHLRDGGGRLLRSWRNGAAGGPAFCEDHAQFASACLTLYETTHDLAWFTAARETADA
                     MLRLFADRERGGFFVTGSDADPLVVRP"
     gene            1684..2340
                     /locus_tag="VIX62_13635"
     CDS             1684..2340
                     /locus_tag="VIX62_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5861317.1"
                     /translation="MIEEIRVLVVDESPGLAQGLILALPRRGPIHVLGPVPDAPEALA
                     ALEESLADLVLVDLDREDGRGAEVVGAIRDGSGRIRVLAASTQDGPETAATALAAGAC
                     GVLPTVRDRSLIDVFRRAVAGELVLPAADLPRLVDRLMGNRDHADGWTSLTGRERQIL
                     RALADGRSTPEIARELGISPLTVQSHVKNILAKLGVHSKVEAVRLAWRNGLGSATRTA
                     "
     gene            2364..3053
                     /locus_tag="VIX62_13640"
     CDS             2364..3053
                     /locus_tag="VIX62_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5861318.1"
                     /translation="MAGHGARADHEAAAARVVIAGAHPVIREVVRRSCMTEGGLEIVA
                     EVTDADGLLEACRREHPDVIVLDVELDDGDGLEALRAIREEGVAAGVLVLTDRTDGAS
                     VLDALKLGVRGYLGKADGLRSVGDCVRRIADGERLIDPDLEQAAVMALGSFARKAREG
                     SEMEASLTPREQEILVMVSDGFTMQQVGTRLGISPRTVETHVAKLYRKLGVRTRVQAV
                     SRAAQLGLIEL"
     gene            3115..3819
                     /locus_tag="VIX62_13645"
     CDS             3115..3819
                     /locus_tag="VIX62_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5861319.1"
                     /translation="MNRTSPEPTAARARRRLGVVLVDPLPVVRAGIALLIDDRPDMEV
                     RAEAGTAAEAIEIVSRVRRSRVVIVVGLGLGGEQDAFWLIRTMRERFPSHAILGCGAN
                     ADPTTISRVLFMGADGFVDKNIDPVEFLQGLRRAADREMVLASPTQGSVGQIAEGLQR
                     RRDFDIKLTEREREVLSVAAEGLTAREIATRLGVRERTVTTHLARIYGKLGVGSRLAA
                     IRLAAQSGLVTVGASE"
     gene            complement(3732..4943)
                     /locus_tag="VIX62_13650"
     CDS             complement(3732..4943)
                     /locus_tag="VIX62_13650"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY5861320.1"
                     /translation="MMGLTTLTLVLGAGLKSPCADRSWVEERRGASGQCYSDIADLLR
                     TEQLADGRLPYLERCDAAAKPCDEYPLVSMYVMRLTASWPGGGDPYTTFFWVNVLLLL
                     ACALAITWCLDRLGAATALFAAAPTLLIYGTMNWDLVPVAFATVATFAFLRRRDVASG
                     ILLGIGAAAKLYPALLLLPFAADRWRADERGRGVKLVLAAGISWLSLNLPFALAAPEG
                     WVHFVRYHSERPAEYDSLWRVACEHGLCLSTSAINVASLVLFAIGSAFVWRLKERRDP
                     DVQAWTFGFVLVAMFLLTNKVWSPQYALWLLPWFALVAPSFKPYLAFQAAEVLVFIAR
                     FSFFQRLEGDGGLPYAALEIAVAARALALVWCLAAWVRTSDYSLAPTVTRPDWAAKRI
                     AAKRLPTPNLP"
     gene            complement(4991..6487)
                     /locus_tag="VIX62_13655"
     CDS             complement(4991..6487)
                     /locus_tag="VIX62_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861321.1"
                     /translation="MANAVRTAPDPSAEAAETTPHPRRPTLDDVWAFLPVAIPMLVSL
                     ASRMVAVDLAYHVRVGEGILAGGGIPRVNTLSFVNEGAAWLDQQWLAQVFIAIAHRGG
                     FATVALLRACLIGGSFGLIYLSCRAKGASPRASSLLSLSGFIVCLQALAMRPQLLAFF
                     LFSASLWILSTRHTHPRRCWALVGIAVVWANVHGSFFLLPLLCALAAGEDLIGGHPGW
                     RRMARFAVGCAVATLVNPFGAGVWSYVVELSTNPVIRQTITEWAPMSVSTFAGTAFFL
                     TAALIAGWLARREGVVPWPALLWLGTFFVLALPARRGVLWWSLVAPVVVAGLLDLRRG
                     TRTMHTGTRWMNLTVIALLGATVLAFLPWSRGLRSTDLLAEAPVGLTAAAREHLPHGA
                     NVLTPQAWGSWFEFDAPEQQVFLDARVELQPQAAWDDYNAVRTARGDWGAILDRWSVD
                     AVVVDARPSWEELEDALAGSSEWCLATSDVAGSLFIRDDAVDGRCIAS"
     gene            6690..6902
                     /locus_tag="VIX62_13660"
     CDS             6690..6902
                     /locus_tag="VIX62_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY5861322.1"
                     /translation="MFLRLYTSATSWWDGLKARAAEEGGATAVEYALMVALIAVVIIA
                     AVAFIGTSSSEQFNSVGTCVDDPAAC"
     gene            7006..7461
                     /locus_tag="VIX62_13665"
     CDS             7006..7461
                     /locus_tag="VIX62_13665"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEY5861323.1"
                     /translation="MERPSNLRRHAQREDGAAAVEFALIVGLLAMLIFGMMEYGLAFW
                     QLQSLRASAREGARVAAVGGDETQIRDAVVGASAGALPAGYTGLTITPIGGCPDPGDA
                     TAVDQSVTVSIDPASLPAGIQQIFSIDIPLLPSFTLNPQIEGTFRCEPV"
     gene            7446..>8219
                     /locus_tag="VIX62_13670"
     CDS             7446..>8219
                     /locus_tag="VIX62_13670"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEY5861324.1"
                     /translation="MRAGLIPGRRSARTRANEESGAVVVITVLALIAIMGMLVLTIDV
                     GGLLLKRRAMVNASDAAALAAAQSCADTGDSFAPEGRADIYASANVDGLLAENGGITE
                     VVGCDTGRGHVTVEYSHPQSLFFAGIFGFGDHGSVTTAATAAWGPAAGGYAVPIVLDS
                     GYLQGVCKVPDGIEIGDTCAFWYNNGDSSLGAANWGFMNLSQWDVDAGTNCHNAGSSD
                     RRDWIVNGYPDIRTLNGNPPGSEPTYVCNDTGHSTSNWST"
BASE COUNT         1378 a         2859 c         2764 g         1218 t
ORIGIN      
        1 ggacgaacca ccaacgggtc ggcgtccgat ccggtcacga agaagccgcc gcgttccctg
       61 tccgcgaaca gccgcagcat cgcatcggcc gtctcgcgcg cggcggtgaa ccacgcgagg
      121 tcgtgcgtgg tctcgtagag ggtgaggcag gccgacgcga actgcgcatg gtcctcgcag
      181 aaggcgggcc ctccggccgc accgttccgc cacgagcgaa ggagccgtcc gcccccgtca
      241 cgcaggtgcg tctgcacgaa cgaggcgcag cgcctcgccg cctcgatgta gcgaggttcg
      301 tcgaaggcgc ggcctgcttc ggccagcgct cggatcacca tcgcgttcca tgccgtgagc
      361 accttgtcgt cggtgccggg gtggacgcgt gcgtcccgtg cctcgaacag gacggtgcgc
      421 gcgtcctcca ccatcgcctc gaggtccgtg ggatcgagtc cgtgttcgcg tgccaccgcc
      481 gaggtcgcga tcggacgcca cagcacgttc gtgcgcccct cctcgccggc ccagttgccc
      541 tcgaccgtgg cgccgaagca cgccgccacc ggctcgccga ccaggtcgac caactcgttc
      601 caggcccacg tgaagaacct gccctcgacg ccttcggagt cggcgtcctg ggaggagaag
      661 aacccgcctt cgggacgctg catctcccgc agcaggtact cgaccgtctc ggtcgccacg
      721 cggcggtaac gatcctcgcc ggtgacctgc caggtccgcg tgtacagctg cacgagctgg
      781 gcgttgtcgt agagcatctt ctcgaagtgg ggaacgtgcc aggcaccgtc ggtggagtac
      841 cgggcgaacc ccccgcccac atggtcgtag atcccgccct ccgccatccg gtcgagcgag
      901 atcaccacca tctccagcgc gtcgggcacg ccgcgcagcg actgccgcag gcagaattcc
      961 agcgtcatcg gctgcgggaa cttcggtgcg ccgccgaacc cgccccagcg gccgtcgaac
     1021 gagggccgca gggcctgcat ggcctcccgg gcgacctccc cggtcaaggg atcgttcgat
     1081 ccttccagcg accccacgct ggcgatcgac tccgtcacct tgccggcctg ggccacgatc
     1141 tcgtcgcgac gctcatccca ggcttcggcg atccccgtca gcacctggcg gaacgacggc
     1201 atcccgggac gcggctccgg tgggtagtac gtgcccgcgt agaacggccg cccgtcgggt
     1261 gtgaggaacg ccgacatggg ccagccgccg tgacccgtca tcgcctgcac cgcatccatg
     1321 tagatgccgt ccaggtcggg tcgctcctcc cggtccacct tgatcgagac gaaggatgtg
     1381 ttcatcaacg ccgcggtcgc ttcgtcctcg aacgactcgc gctccatcac gtgacaccag
     1441 tggcaggccg cgtagccgat cgacaggaag atcggcacat cgcgttcacg tgcggcgttc
     1501 agcgcgtccg gtccccacgg gtaccagtcc accgggttgt gcgcgtgctg caggaggtag
     1561 gggctggtct ggtgcgcgag ccggttcgcc gggcgatcgc tcatgcctcc agtgtctcgc
     1621 gtcctgccca gatgcactcg gctgcttcag gccacccacc accgatgccg atggggtgcg
     1681 gagatgatcg aggaaatccg ggtcttggtg gtggacgaga gcccggggct cgcgcaggga
     1741 ttgatccttg cgctgccccg acgcgggccc atccatgtcc tgggtccggt gcccgacgcc
     1801 cccgaggccc tcgcggcgtt ggaagagagc ctcgccgacc tcgtgctggt cgacctcgac
     1861 cgcgaagacg gccggggcgc cgaggtcgtt ggcgcgatcc gggacggctc tgggcggatc
     1921 cgcgtcctgg cggccagcac ccaggacgga cccgagacgg cagccacggc gctggcggca
     1981 ggagcctgtg gggtgttgcc cacggtgcgt gaccgctccc tgatcgacgt cttccgtcgc
     2041 gccgtcgccg gcgaactcgt cctgcccgcc gccgatcttc cccggctggt cgaccgcctg
     2101 atgggcaacc gcgaccatgc cgacgggtgg acgtcgctca ccggacgtga gcgtcagatc
     2161 ctgagagcgc tcgccgacgg tcggtccact cccgagatcg cgcgcgagct ggggatcagc
     2221 ccgctcacgg tgcagagcca cgtgaagaac atcttggcca agctcggcgt gcactcgaag
     2281 gtcgaggccg tgcggctcgc ttggcgcaac ggcctggggt cggcgacccg caccgcctag
     2341 gtggccgggg tagccttcgt ccgatggccg gtcacggagc acgagcggat cacgaggcgg
     2401 cagcggcgcg cgtcgtcatc gccggggcgc atccggtgat ccgcgaggtc gtgcggcgtt
     2461 cgtgcatgac cgaaggaggt ctcgagatcg tcgcggaggt caccgacgcc gatgggttgc
     2521 tcgaggcctg ccgccgtgag catcccgacg tgatcgtcct ggacgtcgag ctcgacgacg
     2581 gcgacggcct cgaggcgctc cgcgcgatcc gtgaagaagg cgtcgccgcg ggcgtgctcg
     2641 tgctgaccga tcgcaccgac ggcgcctccg tgctcgacgc gctgaagctc ggcgtgcgcg
     2701 gctacctcgg caaggccgac ggactgcgct cggtgggcga ctgcgtgcga cggatcgccg
     2761 acggcgagcg cctgatcgac cccgatctgg agcaggctgc cgtcatggcc ctgggcagct
     2821 tcgccaggaa ggcccgcgaa ggctccgaga tggaggcatc gcttacgcct cgggagcagg
     2881 agatcctggt gatggtgagc gacgggttca cgatgcaaca ggtgggcacg cgcctcggca
     2941 tctcgccacg cacggtggag acacacgtcg caaagctcta ccgcaagctg ggcgtccgca
     3001 cgcgggtgca agcagtctca cgcgccgcgc agctcggctt gatcgagctc tgaacggtcg
     3061 ggcacgcgcc agaaggcgct cacgacctcg cctcaacctg ccgatagcct catcgtgaac
     3121 cgaacgagtc ccgaacccac cgcggcacgg gcccgccgcc gactcggcgt cgtgctcgtc
     3181 gatccgctgc cggtcgtgcg tgccggcatc gccctgctga tcgacgaccg tcccgacatg
     3241 gaggtgcggg ctgaagccgg caccgccgcc gaggccatcg agatcgtcag ccgggtgcgg
     3301 cgctcgcgcg tcgtgatcgt ggtggggctc ggcctcgggg gcgagcagga cgcgttctgg
     3361 ttgatccgca cgatgcggga acggttcccc agccatgcga tcctggggtg cggcgccaac
     3421 gccgacccca cgacgatctc ccgggtgttg ttcatgggcg ccgacgggtt cgtggacaag
     3481 aacatcgacc ccgtcgagtt cctgcagggc ctgcggcgag cggccgatcg cgagatggtg
     3541 ctggcgtctc cgacccaggg ttcggtgggc cagatcgccg agggcctcca gcgtcgccgc
     3601 gacttcgaca tcaagctcac cgagcgcgaa cgtgaggtcc tcagcgtggc cgccgaggga
     3661 ttgaccgcgc gcgagatcgc gacccggctc ggcgtgcggg aacgcaccgt caccacccac
     3721 ctcgcgcgga tctacggcaa gttgggggtc ggcagccgct tggcggcgat ccgcttggcg
     3781 gcccagtccg gcctcgtcac cgtcggcgca agcgagtagt ccgaggttcg cacccaggcc
     3841 gcgaggcacc agaccagcgc caacgctcgg gcagcgaccg cgatctcaag cgctgcgtac
     3901 ggcaggccgc cgtctccttc cagccgctgg aagaacgaga acctcgcgat gaacaccagc
     3961 acctcagccg cttggaaggc gaggtacggc ttgaacgagg gtgccaccaa ggcgaaccac
     4021 gggagcagcc acaacgcgta ctgcggcgac cacaccttgt tcgtcaacaa aaacatcgcg
     4081 acgaggacga atccgaatgt ccacgcttgg acgtccgggt cccgtcgctc cttgaggcgc
     4141 cacacgaacg cgctgccgat cgcgaacaac acgagtgagg cgacgttgat ggcgctggtc
     4201 gacagacaca ggccgtgctc acacgccacc cgccagagcg agtcgtactc ggccggccgc
     4261 tccgagtggt accggacgaa atggacccaa ccctccgggg ccgcgagggc gaacggaagg
     4321 ttgagggaga gccacgagat gccggcagcg agcaccagct tcacccctct gccgcgttcg
     4381 tcggcgcgcc agcgatcggc ggcgaacggc aacagcaaca gcgccgggta gagcttggcg
     4441 gccgctccga tgccgagaag gatgccgctc gcgacatcgc gccgccggag gaatgcgaac
     4501 gtcgccaccg ttgcgaacgc caccggcacg agatcccagt tcatcgtccc gtagatcagc
     4561 agcgtgggag cggccgcgaa caacgcggtc gccgcaccga gccgatcgag gcaccacgtg
     4621 atcgcgaggg cgcaggccag cagcagtaag acgttgaccc agaagaaggt cgtgtacggg
     4681 tcgccgcctc cgggccatga agcggtcaag cgcatcacgt acatcgagac gagcgggtac
     4741 tcgtcgcatg gcttcgccgc cgcgtcacat cgttcgagat acggtaggcg gccgtcggcc
     4801 aattgctcgg tccgaaggag atcggcgatg tcggagtagc actggccgct ggccccgcgt
     4861 cgttcttcga cccacgatcg gtcggcgcac ggggacttga gcccggcccc gaggaccagg
     4921 gtcagcgtcg tgagacccat catggccgcg agggtcaatg atgatccccg gcggaacctg
     4981 cccgtgctac tcatgacgcg atgcagcgac cgtcaacagc gtcgtctcgg atgaacaggc
     5041 tccctgccac gtcgctcgtg gccagacacc actcagatga cccggccagc gcatcttcca
     5101 gctcctccca gctggggcgg gcatccacca cgaccgcgtc gacggaccat ctatcgagga
     5161 tggcgcccca gtcgcctcgg gcggtgcgga ccgcgttgta gtcgtcccag gccgcttgcg
     5221 gctgcagttc gacccgtgca tcgaggaaca cctgctgctc gggggcatcg aattcgaacc
     5281 acgatcccca ggcctgaggt gtgagcacgt tcgccccatg tggcaggtgc tcccgggcgg
     5341 cggcggtgag tccaaccggc gcctctgcga gcaggtccgt ggaacgcaag cctctcgacc
     5401 atggcaggaa ggcaagaacg gtcgccccga ggagcgcgat caccgtcagg ttcatccaac
     5461 gtgtaccggt gtgcatcgtc cgtgtgcccc ttcgaaggtc caggagaccg gccaccacca
     5521 cgggcgccac gagggaccac cacaggactc cgcgccgggc cggcaacgcc aggacgaaga
     5581 aggttccgag ccagagcaac gcgggccagg ggacgacgcc ctcgcgccga gccagccatc
     5641 ctgcgatcag cgctgccgtg aggaagaacg ccgtccccgc gaacgtcgag accgacatcg
     5701 gagcccactc cgtgatcgtc tgtcggatga cggggttcgt ggagagttcc acgacgtagc
     5761 tccacacgcc ggcgccgaac gggttgacca atgtcgctac ggcgcacccc acggcgaacc
     5821 gggccatcct tcgccatccc ggatgtcctc cgatgagatc ctccccggcc gcgagcgcac
     5881 agaggagggg aaggaggaag aagcttccgt gaacgttcgc ccacaccacc gcgatcccaa
     5941 ccagcgccca acaccgcctg ggatgggtgt gccgggttga caggatccac aggctcgcgg
     6001 agaacaggaa gaacgccaag agctgcggtc gcatggccag tgcctggagg cagacgatga
     6061 acccgctgag cgacaggagc gaggacgctc gtgggcttgc tcctttcgct cggcaggaca
     6121 ggtagatgag cccgaacgac cccccgatca ggcacgcgcg gaggagtgcc accgtcgcga
     6181 acccgcctcg gtgggcgatc gcgatgaaca cttgggcaag ccactgttga tccagccatg
     6241 ccgctccctc attcacgaac gaaagggtgt tgacgcgcgg gatcccaccc cccgccagga
     6301 tcccctcgcc cacgcgaacg tgatacgcca ggtccaccgc caccatccgg ctcgcgaggg
     6361 acaccagcat cggtatcgcc acgggcaaga acgcccacac gtcgtcgagc gtcggccgac
     6421 gtggatgcgg ggttgtctcc gcggcttcgg ctgagggatc cggcgcggtg cgcacagcgt
     6481 tcgccatccc gattccatcg gtcggcgagc cggggtgctg gagtccacgc acgtgcgtac
     6541 gtaggactac gtagccaccc tccgggaaac acggaattcc ctggtcagac cgcggaatct
     6601 accgtggtgt ccgcactcaa gcttcacggc tgagaggtcg aaaaccctcc cagcaaggac
     6661 ggatctttcg gaggaagggg gtgtagcaca tgtttctgcg tctctacacg agcgcgacct
     6721 cttggtggga cgggctcaag gctcgggccg ctgaggaagg tggcgcgacc gcggtggagt
     6781 acgcgctgat ggttgccctg atcgcggtgg tcatcatcgc ggcagtggcc ttcatcggca
     6841 cgagctccag cgagcagttc aactcggtcg gcacctgcgt cgacgacccg gccgcctgct
     6901 aaggcgacag ggaagcatga gcggctgtac gccggccgct cactctcccg gatccacgcc
     6961 gagagagtga gggaccggcg cacgagcgag cgaggtatca cgaagatgga gagaccttcg
     7021 aaccttcgcc gtcatgccca gcgggaagac ggcgcagcgg cggtggaatt cgcgttgatc
     7081 gtcggactcc tcgccatgtt gatcttcggc atgatggagt acggcctcgc cttctggcag
     7141 ctccagagcc tgcgcgcatc ggctcgagag ggcgcccgcg tcgcggccgt cggcggcgat
     7201 gagacccaga tccgcgacgc cgtggtcggc gcctcggccg gggcgcttcc cgccggctac
     7261 accggtctca cgatcacgcc catcggcggg tgccccgacc ccggggacgc cacggccgtc
     7321 gaccagtcgg tcacggtgtc gatcgatccc gcgagcttgc cggccggtat ccaacagatc
     7381 ttctcgatcg acatcccctt gctgccgtcg ttcaccttga acccgcagat cgaagggacc
     7441 ttccggtgcg agccggtctg atcccaggtc ggcgctctgc acggacccgc gcgaacgagg
     7501 agtcgggcgc ggtcgtcgtg atcaccgtgc tggccctgat cgccatcatg ggcatgctcg
     7561 tgctcacgat cgatgtgggc ggtctgctcc tcaagcgacg cgccatggtg aacgcatccg
     7621 atgcggccgc cctggccgcc gcccagtcgt gcgccgacac cggtgattcc ttcgcgcccg
     7681 agggtcgcgc cgacatctac gcctcggcca acgtcgacgg cctcctggcc gagaacggtg
     7741 gcatcaccga ggtggtcggc tgtgacaccg gccgtgggca tgtcaccgtc gagtactccc
     7801 acccccagag cctgttcttc gccgggatct tcgggttcgg cgaccacggg tcggtgacca
     7861 cggcggcgac cgccgcgtgg ggacccgccg cagggggata cgccgtgccg atcgtcctgg
     7921 actccggcta cctccagggc gtctgcaagg tccccgatgg gatcgagatc ggtgacacct
     7981 gcgccttctg gtacaacaac ggcgattcga gcctcggcgc cgccaactgg gggttcatga
     8041 acctcagcca gtgggacgtc gacgccggca ccaactgcca caacgccggg tcctcggatc
     8101 gtcgggactg gatcgtcaac ggctatcccg acatccgcac cctcaacggg aaccctccgg
     8161 gcagcgagcc gacctacgtc tgcaacgaca cgggccattc cacgtcgaac tggagcacg
//
LOCUS       DATDBZ010000591        13777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1839258, whole genome shotgun sequence.
ACCESSION   DATDBZ010000591 DATDBZ010000000
VERSION     DATDBZ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13777
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839258"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..891
                     /locus_tag="VIX62_13675"
     CDS             109..891
                     /locus_tag="VIX62_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5861325.1"
                     /translation="MSAVAPLPVAPAWFELNRGEHDVARLQELRLDRMLESNVWHVRG
                     AKADLVIDTGNGIGRLRPQLEWFHPEQPMIAVATHGHFDHTGGMAEFEDRRCHEADAG
                     EVREPYPLTLVRERYPDDLDEIFAYYGYETPDVLVTAVPWEGFDVAAWTIRGCDPTSF
                     VADGDVIDLGDRTVEILHVPGHTPGSIALWEPATGLLFTGDMVYADDRLSFDDWEATE
                     RSLARLRALPVTLVHPGHGRSIERDEFVSLIDEVVAAGPATH"
     gene            complement(908..2524)
                     /locus_tag="VIX62_13680"
     CDS             complement(908..2524)
                     /locus_tag="VIX62_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861326.1"
                     /translation="MVSLRTEPTFSHKAAALAAITALAVSILAVGSPVAHGGPTHEVT
                     EFTLPVDGSSPFGIATGPDGALWFTARGTDSIGSLATDGTFGPTTALALTSDPTAITA
                     GPDDAVWFTEQGSGLIGRVTTDGTLTEFFLPSDGAGPAGIVTGPDGALWFTERSAHRV
                     ARMATDGTTVEFELPTPVAGPVGIALGSDGNLWFTEQRANQIGRITTDGEVTEYPVPV
                     ASSLPTGIAAGPDGALWFTMRAANRIGRIDTAGNVTTFAIPTEASDPTGIAAGWDGAL
                     WFTEPDADAIGRITTGGTIKEFSLPNVGSSPFGITGGPDDAVWFTEGNGNRIGRLSAV
                     DAPIDTTAPTISITSPIEGSVFEQGQQIVAGYGCADEAGGSGLATCAGPVIDGQAIDT
                     SIGSHRFDVEASDVQGNTATASTGYIVFSDLGGSLLTPTTRRAGFWETLDLGLGLTRP
                     ADALLFVAPGYPRSQQVSCADPSQAIGEPEPADVKLQLKQGGLDVRWRTDREWTGSCR
                     AFVLRFTPEGWSGTDAVFVARFGTAQSKKH"
     gene            2619..3857
                     /locus_tag="VIX62_13685"
     CDS             2619..3857
                     /locus_tag="VIX62_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5861327.1"
                     /translation="MAADELVLIRAGRFIDVLSGEVRARQVVLVRGDRVESVGEDDGS
                     VPEGARVIDLSNATVLPGLMDMHAHLVGLEEDGQGYASLVMRSGAQDAFGGVRNARST
                     IMAGFTTVRDIGTFRAFVDVALRDAIEAGDVLGPRMRCAGAYVTCPGGGGDLTGLAVD
                     VDSVIPRELRFGVTSGVDQMRSNVRNILRYGADFIKIIATGAVLTSGTNPGAPEFTED
                     ELRAAVEETALSGTFVAAHAHGAEGIKRAVRAGVRSIEHGSLMDDESIEMMAEAGTYL
                     VADIYDGDWIGQEGPRMGYSDEVLRKNEETTQAQRDGFRKCVKAGVKIAYGTDSGVYP
                     HGLNARQFVKHVEHGQTPMEAIQAATIRCSELLEWDEVGAVAPGRYADLIALETDPIE
                     DVAALQDVAFVMKGGEVVKG"
     gene            3925..5529
                     /locus_tag="VIX62_13690"
     CDS             3925..5529
                     /locus_tag="VIX62_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5861328.1"
                     /translation="MSDRSYDAVIVGGGHNGLVAGYYLARAGMKTVVLERRSFVGGAC
                     NTEEFAPGFRASTGAYVLSMLRESIWQDMQLERRGIHVDAAGPTLNLYPDGAHYYLGD
                     DMAANIEETKRFSAADARALPVFEDHLSELVQGVLPAFNWTAPDPRIRSLHDLLELAK
                     WGRIGFKQRKHLTDLAFLFSTSANQLLSERFTDDHVKAAMGWHAINDSVAGPSTPGTA
                     FVLLHDHASEEADGGVRQWGFVRGGMGVLTATMADAAREVGCEIRCDAEVDQILTRGG
                     RAVGVRLGDGEELRAARVLSNADPKRTFLSLCDRAELPAEFVARIEAYRCMGTSIKIN
                     LAVSELPYVRGLPEGGVQPYHTGIMEMDPFIADMDFQQAQAVQGIPADPAHIEVCFPT
                     VHDPSLAPEGKHIMTIDVNSQPYHLRDERWDDIKEARADRAIAQMSEHFPKLPDLIEH
                     RQVVTPLDMERLMGLTGGHALHGDMSPDQLLFLRPVRGWADYRTPIRGLYLCGAGTHP
                     GGGVTGANGRNAVREVLKDAGKRTKR"
     gene            complement(5552..6544)
                     /locus_tag="VIX62_13695"
     CDS             complement(5552..6544)
                     /locus_tag="VIX62_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861329.1"
                     /translation="MRNRSSGRMTRASAAAAFVAVALALLPATAAVGGGTPSLVPEKV
                     IGGPSDTFAPTSNGTWIAWSQWDHATEQTFVRRLDSTTQRRLNERGTGGSMGGFVGSS
                     NTLIYQQFHGYRSDLYFYDVQTRDRTKLPAVINTSDWEYWPLASNAYILFARLHRRAD
                     HRTAWRRLFLFDRMSESLRPLTKQLRPSTTLSPDVIGETSAGWTVCAAACNVWIWDAV
                     TDTIEKLPNPLGMDQYGAAIDEAAGTITYSRDRSGRCGRNGTLRLMQIGSGTSTTLTT
                     IPDGLSLGIRRTMVENPDTGNLDLYVSRYRCDAKPTPSGRSPADILVYRDIDLP"
     gene            complement(6654..7955)
                     /gene="efeB"
                     /locus_tag="VIX62_13700"
     CDS             complement(6654..7955)
                     /gene="efeB"
                     /locus_tag="VIX62_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HEY5861330.1"
                     /translation="MNDENDRAAAQSGDPHDPAAISRRRFLSTVGAGVGAAATIGGIS
                     GYALASEREATATAPISTTVPFFGPHQAGVTTPAQAHMAFVSFDVMPTADRGEVEALL
                     ATWTAAAAAMCAGEPVGTTDEGARLAPPEDTGEAVGLPAAALTVTFGMGPSLFTGELG
                     RRLGLQDRRPGSLIDLPPFPGDVLDPRRSDGDLGIQVCAEDPQVVMHAVRNLVRIGRG
                     TVLARWIQQGFIPATHDETPRNLMGFKDGTNNPDIASDAAMDAAVWVPQEERQAWMRG
                     GTYLVFRRIRILVEFWDRTILDEQERTIGRHKGSGAPMGAAAEFDPLPLEAKDADGEP
                     AIPIDAHVRLAAPAMNRGATMLRRGYSFADGLDPVTGEIDAGLLFLAFQQHPETQFVR
                     VQNALAGVDALNEYIKHVGSAIFAVPGGIAPGASVGDALFA"
     gene            complement(7952..9115)
                     /gene="efeO"
                     /locus_tag="VIX62_13705"
     CDS             complement(7952..9115)
                     /gene="efeO"
                     /locus_tag="VIX62_13705"
                     /inference="COORDINATES: protein motif:HMM:NF041757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEY5861331.1"
                     /translation="MGGDDVPMVKRGRFRVPAAMAVAGALILSACGSNDDPQAGEGEN
                     ADEQIQVVAVQLTDQGCSPTTAEAAAGPTTFEATTDRTDEPELEILDGDLLLGEVANL
                     APGLTGTFSLTLEPGEYQMRCGRHSDPVTGTLTVSGGVGAHTSEAADAAVETYRTFAL
                     GQTKILIARTQEFADAVLAGDIAEAKDLFAYAREPYERIEPIAETFGDLDPAIDAREG
                     DVPEAKWTGFHRIEKALWVDEGLTGMAPVAKKLVADVRLLQELTRQVDLEPAQVANGA
                     SELLGEVANSKITGEEDRYSHTDLWDFEANVEGSKAAFDAVKPLIPADQAALVAEIEA
                     GFVDAVAVLDPYRKGDGFVLYTDLTEEDTTTLAQTIDTLAEPLSQVAAIVVAP"
     gene            complement(9258..9782)
                     /locus_tag="VIX62_13710"
     CDS             complement(9258..9782)
                     /locus_tag="VIX62_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861332.1"
                     /translation="MTDQADERALLLGMPLVHLRSAAETCERHDRVVLPAGGPGDLDR
                     TAPGATVLLMASDAGEDEVSAATWAARFVGRVHYEPGDPLPTMLPATWVETREADALA
                     RPDEPAEVNEDEEDEDEDEDEDRLGPQSFLVVEELRELPMQEWVFTNELVGKQHRGGR
                     TFFPRGPTLVRPPA"
     gene            9917..11410
                     /locus_tag="VIX62_13715"
     CDS             9917..11410
                     /locus_tag="VIX62_13715"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEY5861333.1"
                     /translation="MTDRQDELDVYADGQGRRGQVDRRSFIRAGVLGTAMASSVLMTS
                     DLAAGAAMLKRSTRTATPRGSGLVQAGPELALPPGFSYHTFGAFGSAMSDGFITPPIH
                     DGMAAFDVGGGKLRIVRNHELGEGNDIPPGTVIGDPATAWDKRAPGGTTTIEVDATTA
                     EYLGSWISLNGTDTNCAGMPTPWGTWITCEETTIGVDAGYKKPHGYVFEVDPLADESV
                     LTKPYKKLGRFLHEAGAVDPDTGVVYMTEDEGPDGFYRFVPETLGDLTSGQLQMLRVK
                     GQPKYNTIVGQTVGAVLVCNWVNINTPDPSDAEDNASAVFHEGRSKGGAKFLGGEGCT
                     YRDGGVVFGSSDGGDKGLGQIWQYTPTNNVGELNEQGELVLLFESGGRQTLDGPDNMT
                     TSPGGAIVIAEDGNLKSNFVRGLLPNGTMFPIAENLVAVQRHYIEASGKVYDPTVPDD
                     GPSAGDGTGFSEFAGPTFSPDGEWLFVNIQVPGITCAITGDWASLGL"
     gene            11407..12762
                     /locus_tag="VIX62_13720"
     CDS             11407..12762
                     /locus_tag="VIX62_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5861334.1"
                     /translation="MSRDDGSNGAVPKIVTTPPGPKTRAILERQSAVLYRGLAEEVGP
                     FVICRKSGHEIEDVDGNVYLDFISAMASVPAGAARADITDAAVDALRRYGNEDTHFYS
                     NEYVLPLAERLVAIAPGALSRVDIALNGTEAVETSVRFMRRTTGRPIVIGFMGGYHGE
                     AGTAGAIGAESSDLSFGYRALMPGFVHVPYPNPYRTPFTARAGGTGDGTIDYLRDHVL
                     HHAVDPREVAGFVIEPVMGSGGCVAPPDGFWSALTDLCREFDFLLCADEVKTGFGRTG
                     HMFAVERWGVEPDLMALGKSMGGGVMPIGAVLGTERSMDFDDVSTGSTWSWLPAACAA
                     ALETLDVFEREPVLENVRVLEAAGLQALEGLMDRYPAIGDVRAVGCFLAVEFVKDRAT
                     KERDLDLQERVAWGCLRRGLLVDPSTTSLNLQPSLTMPVEVMGTAMAILDEAIAEAVV
                     A"
     gene            12759..>13777
                     /locus_tag="VIX62_13725"
     CDS             12759..>13777
                     /locus_tag="VIX62_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5861335.1"
                     /translation="MTSVSTGHVDDLAASVRRDLVELVEIPSVSGEEHAVVDHLERRC
                     AELGLPCRRMPVDGGADDLLIGWHERPSLLITAHMDTIRPTWEWSGVAEVRDGRVIGL
                     GAQDDKACVVAALHGLLMAREAGVPLETLPVGIGLCVDEEIGGTGSISMARTLRPPFV
                     VGLEGTELQLGTAEAGFVEVWCHLRGVAVHGALRELGENAIDTAHALIGRIQEHPGSQ
                     HEHPLLGRNIPMIWEIRGGQRLNVVPDRCSFHLDWRVTPGGPSAASLLAWLEEATADA
                     GGDVELVEVIEPFETDPGAEVVTALGNAVEAATGKRPAPTGMVAWTDAHNFVDLGGSQ
                     AVVFGP"
BASE COUNT         2078 a         4609 c         4891 g         2199 t
ORIGIN      
        1 cgagggagac atcgtggcgc tgccgaacgt gggcagctac aacgcctcga tgtccagcgc
       61 gcattgtctc cgtgggccgg cggcgtcgat cgccttcccg gagcgggcgt gagcgcggtg
      121 gccccgctcc ccgtcgcccc cgcttggttc gagctgaacc gcggcgagca cgatgtggcg
      181 agactgcagg agctcaggct cgatcggatg ctggagtcca acgtctggca cgtgcgaggg
      241 gcaaaggccg acctggtgat cgacaccggc aacggcatcg ggcggctgcg accgcagctg
      301 gagtggttcc atccggaaca gccgatgatc gcggtggcga cgcacgggca tttcgatcac
      361 acgggcggca tggcggagtt cgaggaccgt cgctgccatg aggccgacgc gggcgaggtc
      421 cgcgagccgt atcccctgac gctggtgcgc gaacgctacc ccgacgacct cgacgagatc
      481 ttcgcctact acgggtacga gacgcccgac gtgttggtga ccgccgtgcc gtgggaaggc
      541 ttcgacgtgg ccgcgtggac gatccgcggg tgcgacccga cgtcgttcgt ggccgacggg
      601 gacgtgatcg acctggggga ccgaaccgtg gagatcctgc acgtccccgg tcacacgccg
      661 gggtcgatcg cactctggga accggccaca gggctcctgt tcaccgggga catggtctac
      721 gccgacgacc ggctctcctt cgacgactgg gaggccacgg aacgttcact ggcccggctc
      781 cgggcgttgc ccgtgacgct ggtgcatccg gggcacggcc gcagcatcga gcgtgacgag
      841 ttcgtgtcgc tgatcgacga ggtggtggcc gcggggccgg cgacccacta ggcgtgcagg
      901 ccggtggtca gtgcttcttc gactgcgcgg tgccgaatcg ggcgacgaag acggcgtcgg
      961 tgccgctcca cccttccggc gtgaaccgga gcacgaacgc acggcagctg ccggtccatt
     1021 cccgatcggt tcgccagcgg acgtccagtc ctccctgctt cagctggagc ttcacgtccg
     1081 ccggttccgg ttcgccgatc gcctgcgacg gatccgcgca gctcacctgc tgggaccggg
     1141 ggtagccggg cgctacgaac agcagggcgt cggccgggcg ggtgagtccg agcccgagat
     1201 ccagcgtctc ccagaacccg gcgcgccgag tcgtgggcgt gaggagcgac cctccgaggt
     1261 cggagaacac gatgtagccg gtcgaggccg tcgccgtgtt cccttggacg tctgatgcct
     1321 ccacgtcgaa gcggtgggac ccgatcgagg tatcgatcgc ttgcccgtcg atcaccggcc
     1381 ctgcgcacgt cgccaggccg gagccgcctg cttcgtcggc gcagccgtat ccggccacga
     1441 tctgctgccc ctgctcgaac acgctcccct cgatggggga ggtgatcgag atcgtgggag
     1501 ccgtcgtgtc gatcggggca tccaccgcgc tcagccggcc gatgcggttc ccgttgcctt
     1561 cggtgaacca cacggcatcg tcgggtccgc cggtgatccc gaacggggac gagccgacgt
     1621 tcggcagcga gaactccttg atcgtgccgc ccgtcgtgat ccgtccgatc gcgtcggcgt
     1681 caggctcggt gaaccagagc gcgccgtccc atcccgcagc gatccccgtg gggtcactcg
     1741 cttcggtggg gatcgcgaac gtcgtgacgt tgccagcggt gtcgatccgt ccgatgcggt
     1801 tggcggcgcg catcgtgaac cacagcgcgc cgtcgggacc ggctgcgatc ccggtgggga
     1861 ggctggaggc gacgggcacc gggtactcgg tcacctcgcc gtcggtggtg atgcgaccga
     1921 tctggttcgc acgctgctcg gtgaaccaca ggttgccgtc cgacccgagt gcgatcccca
     1981 ccggaccggc gaccggggtg ggcagctcga actccacggt ggtgccgtcg gtcgccatcc
     2041 gcgccacgcg atgcgcgctg cgctcggtga accacaaggc cccatccggt ccggtcacga
     2101 tccccgcggg tccggcaccg tcggatggga ggaagaactc ggtcagcgtt ccgtccgtcg
     2161 tgacgcgacc gatcaggccg gacccctgct cggtgaacca caccgcgtca tcgggcccgg
     2221 ccgtgatcgc ggtggggtcc gatgtcaatg cgagcgccgt cgtgggcccg aacgtcccat
     2281 ccgtcgccag ggaaccgatc gagtcggtgc ctcgggcggt gaaccacaac gcgccgtccg
     2341 gtccggtcgc gataccgaag ggcgaggagc cgtcgacggg cagcgtgaac tccgtcacct
     2401 cgtgggtcgg gcccccatgc gcgaccggtg accccaccgc caggatggag accgccagcg
     2461 ccgtgatcgc cgccaaggcg gccgccttgt gcgagaacgt gggctcggta cgcagggata
     2521 ccatgtttcg ggtatcggtc cgcggggccc tacggcgcat cgaccgaagg tccatacctc
     2581 ccgtcagcca ttcgaccgat gaagctatgc tccgcgccat ggctgccgac gaactcgtgc
     2641 tgatccgggc cggacgcttc atcgatgtgc tgtcgggcga ggtgagagca cgccaggtgg
     2701 tgctcgtgcg cggcgaccgc gtcgagtccg tgggcgagga cgacggatcg gtgcctgagg
     2761 gcgcgagggt gatcgacctg tcgaacgcca cggtgctgcc cggcctgatg gacatgcatg
     2821 cccacctggt cgggctggag gaggacggcc agggctatgc gtcgttggtg atgcgctccg
     2881 gtgcccagga cgcgttcggc ggggtgcgca acgcgcgcag cacgatcatg gcgggcttca
     2941 ccacggtccg ggacatcggg acgttccggg ccttcgtcga tgtggcgttg cgcgatgcga
     3001 tcgaggccgg cgacgtgctg ggtccacgga tgcgctgcgc aggcgcgtac gtgacgtgcc
     3061 cgggcggcgg tggcgacctc accgggctcg cggtcgacgt cgactcggtg atccctcgcg
     3121 agctgcgatt cggtgtgacg tccggggtcg accagatgcg ctcgaacgtg cggaacatcc
     3181 ttcggtacgg cgcggacttc atcaagatca tcgcgacggg tgcggtgctg accagcggca
     3241 cgaacccggg cgcgccggag ttcaccgagg acgagctgcg ggcggccgtc gaggaaacgg
     3301 cgctgtcggg aacgttcgtt gcggcgcatg cgcatggcgc cgaggggatc aagcgcgcgg
     3361 tgcgcgccgg cgtgcggtcg atcgagcacg gatcgctgat ggacgacgag tcgatcgaga
     3421 tgatggccga ggccggcacg tacctggtgg ccgacatcta cgacggcgac tggatcggcc
     3481 aggagggacc ccgcatgggc tactccgacg aggtgctccg caagaacgag gagaccaccc
     3541 aggcccagcg tgacgggttc cgcaagtgcg tgaaggccgg cgtgaagatc gcctacggca
     3601 cggacagcgg cgtgtatccc cacgggttga acgctcgcca gttcgtcaaa cacgtggagc
     3661 acggacagac gccgatggag gccatccagg cggcgacgat ccggtgttcg gagctcttgg
     3721 agtgggacga ggtgggggcg gtggcccccg ggcgctacgc cgacctgatc gcgctggaga
     3781 cggatccgat cgaggacgtc gccgccctcc aggatgtcgc cttcgtgatg aagggcggcg
     3841 aggtcgtgaa ggggtgaccc ttccgaggtc gccgcacgtc cagatggttg acagtcaaca
     3901 atctccccgc ctagagtccg ccgcgtgagc gaccgttcgt acgacgccgt catcgtcggc
     3961 gggggccata acggcctggt cgccggctac tacctcgccc gggccgggat gaagaccgtg
     4021 gtgttggaac ggcggtcctt cgtgggcggc gcctgcaaca ccgaggagtt cgcccccggg
     4081 ttccgcgcct ccaccggcgc ctacgtgctc tcgatgctgc gcgagtcgat ctggcaggac
     4141 atgcaactcg agcgtcgcgg catccatgtc gatgccgcgg ggccaaccct caacctctat
     4201 cccgacggcg cccactacta cctgggcgac gacatggcgg ccaacatcga ggagacgaag
     4261 cggttctcgg cggccgacgc ccgcgccctc ccggtgttcg aggatcacct ctccgaactg
     4321 gtccaggggg tgctgcccgc cttcaactgg acggcgcccg acccgaggat ccgatccctg
     4381 cacgatctgt tggagcttgc gaagtggggg cggatcggat tcaagcagcg caagcacctc
     4441 accgacctcg cgttcctgtt ctcgacctcg gcgaaccaac tcctgtccga gcgtttcacc
     4501 gacgaccacg tgaaagcggc gatgggctgg cacgcgatca acgacagcgt cgccggtccg
     4561 tccaccccgg gcaccgcgtt cgtgctgctg cacgaccacg cgagcgagga ggccgacggc
     4621 ggcgtccgcc aatgggggtt cgtgcggggc ggcatgggcg tgctcacggc gacgatggcc
     4681 gatgccgctc gcgaggtggg gtgcgagatc cggtgcgacg ccgaggtcga tcagatcctc
     4741 acccggggcg gtcgcgcggt gggcgtgcgg ctcggcgacg gcgaggaact ccgcgcggcc
     4801 cgcgtactgt cgaacgccga ccccaagcgc acgttcctgt cgttgtgcga tcgcgccgag
     4861 ctgcctgccg agttcgtcgc ccggatcgag gcctaccgct gcatgggcac cagcatcaag
     4921 atcaacctcg ccgtcagcga actgccgtac gtgcgcggcc ttcccgaggg cggcgtgcag
     4981 ccgtatcaca ccgggatcat ggagatggat ccgttcatcg ccgacatgga cttccagcag
     5041 gcgcaggccg tgcaggggat ccccgccgac cccgcgcaca tcgaggtgtg cttccccacg
     5101 gtgcacgacc cttcgctcgc acccgagggc aaacacatca tgacgatcga cgtgaactcc
     5161 cagccctacc acctgcgcga tgagcggtgg gacgacatca aggaagcgcg ggccgatcgc
     5221 gcgatcgccc agatgtccga gcacttcccc aagctgcccg acctgatcga acaccggcag
     5281 gtggtgacgc cgctcgacat ggaacggctc atgggcctca ccggcggcca cgcgctgcac
     5341 ggcgacatgt cgcccgatca gttgctgttc ctgcgcccgg tccgcgggtg ggccgattac
     5401 cgaacgccga tccgcggcct gtacctgtgc ggtgccggca cgcatcccgg cggcggtgtc
     5461 accggcgcga acggtcgcaa cgccgtccgc gaggtcttga aggacgccgg caagcgcacc
     5521 aagcgctgag caagcagcac caagagccgg ttcagggcag gtcgatgtcg cggtacacga
     5581 ggatgtcggc gggagagcgc ccggagggtg tcggcttcgc gtcgcaccgg tatcggctga
     5641 catagaggtc aaggttgccg gtgtcggggt tctcgaccat cgttcgacgg atgcccaggc
     5701 tgagcccatc tgggatcgtc gtgagggtgg tcgaggtgcc cgatccgatc tgcatcagcc
     5761 gcagggttcc gttccggccg catcgtccgg accggtcacg ggagtaggtg atcgtcccgg
     5821 cagcctcgtc gatcgcggcg ccgtactggt ccatccctag cgggttcggc agcttctcga
     5881 tcgtgtcggt cactgcgtcc cagatccaga cgttgcaggc tgccgcgcaa acggtccagc
     5941 cagcggaggt ttcaccgatg acatctggac tcaaggtggt gctggggcgg agctgcttcg
     6001 tgagcgggcg aaggctctcg gacatccggt cgaagaggaa caagcgacgc cacgcggtcc
     6061 ggtgatcggc gcggcggtgc agtcgggcga acaggatgta tgcgttgctc gcgagtggcc
     6121 agtactccca gtccgatgtg ttgatgacag cagggagctt cgtccgatcc cgggtctgga
     6181 cgtcatagaa gtagagatcc gaacgatatc cgtggaactg ctgatagatc agggtgttcg
     6241 agcttccgac gaagccgccc atcgagccgc cggtccctcg ctcgttgaga cggcgttgcg
     6301 tcgtcgagtc cagcctccgc acgaacgtct gctcggtcgc gtggtcccac tggctccagg
     6361 cgatccacgt gccgttggac gtcggggcga acgtgtcgct gggtcctccg atgaccttct
     6421 cgggtaccag gctcggcgtg ccgcctccga cagctgccgt tgccgggagg agcgcgagag
     6481 cgacggcgac gaacgcggcg gccgcgctcg ctcgtgtcat ccgacccgag ctgcgattcc
     6541 tcatcgtggg acctcctcct tacgcatgaa cgacgcatga acgcttgaag tgacgatgcc
     6601 gcttacagag ggagcgggga accgctcaac ggcgcccgga gaacgaggtc ggattaggcg
     6661 aacagcgcgt cgcccaccga cgcgcccgga gcgatcccgc ccggcaccgc gaagatcgcg
     6721 ctcccgacgt gcttgatgta ctcgttcagc gcgtcgaccc cggctagggc gttctgcacg
     6781 cgaacgaact gggtctcggg gtgctgttgg aaggccagga acaacagccc ggcatcgatc
     6841 tcgccggtga ccgggtcgag gccgtcggcg aaggagtacc cgcgccgcag catggtcgcg
     6901 ccgcgattca tcgccggcgc cgcgaggcgg acgtgggcat cgatggggat cgccggctcg
     6961 ccgtcggcgt ccttcgcctc gagcggcagc gggtcgaact ccgcggccgc gcccatcggc
     7021 gcgccgctgc ccttgtgccg gccgatcgtg cgttcctgct cgtccaggat cgtgcgatcc
     7081 cagaactcga cgaggatcct gatgcgtcgg aacaccagat aggtgccgcc gcgcatccat
     7141 gcctgtcgtt cctcctgcgg cacccacacc gcggcgtcca tcgccgcatc gctcgcgatg
     7201 tcggggttgt tcgtcccgtc cttgaacccc atgaggttgc gcggcgtctc gtcgtgcgtc
     7261 gccgggatga agccctgctg gatccagcgc gcgagcacgg tgccgcgtcc gatccgcacc
     7321 aggttccgca ccgcatgcat cacgacctgc gggtcctcgg cgcacacctg gatgccgagg
     7381 tcgccgtcgc tgcggcgcgg gtcgaggacg tcccccggga acgggggcag gtcgatcaac
     7441 gatcccggcc ggcggtcctg gagcccgagc cgccggccca actcgcccgt gaacagggac
     7501 gggcccatcc cgaacgtgac ggtgagcgcc gcggcgggca ggcccaccgc ctcgcccgtg
     7561 tcctcgggcg gcgcgagccg ggccccttcg tcggtggtgc cgacgggttc gcccgcgcac
     7621 atcgcggccg ccgccgcggt ccaggtggcg agcagcgctt cgacctcgcc tcgatcggcc
     7681 gtgggcatca cgtcgaacga cacgaacgcc atgtgcgcct gagcaggcgt cgtcacgccc
     7741 gcctgatgcg gcccgaagaa cgggacggtc gtcgagatcg gcgcggtggc cgtcgcctcg
     7801 cgttcggacg cgagcgcgta gccgctgatc ccgccgatgg tagccgccgc cccgacgccg
     7861 gccccgacgg tgctcaggaa ccggcgccgg gagatcgcgg cggggtcgtg cgggtcaccg
     7921 ctctgggcgg cggcccggtc gttctcgtcg ttcatggtgc cacgacgatg gcggccacct
     7981 gcgacaacgg ctcggccagc gtgtcgatcg tctgcgcgag cgtggtggtg tcctcctcgg
     8041 tcaggtcggt gtacaggacg aagccgtcac ccttccgata ggggtcgagg acggccaccg
     8101 cgtcgacgaa gcccgcttcg atctcggcga cgagcgctgc ctggtcggcc gggatcaacg
     8161 gcttcacggc gtcgaaggcg gccttcgagc cctcgacgtt ggcctcgaag tcccacaggt
     8221 cggtgtgcga atagcggtct tcttcgccgg tgatcttgga gttggcgacc tcgcccaaca
     8281 attcgctggc tccgttcgcg acctgggcgg gttcgaggtc gacctggcgc gtgagttcct
     8341 gcaacaggcg cacgtcggcc acgagcttct tcgcgacggg agccatgccc gtgaggccct
     8401 cgtcgaccca gagcgccttc tcgatccgat ggaagccggt ccacttcgcc tcggggacgt
     8461 cgccctcgcg ggcgtcgatc gcggggtcga ggtcgccgaa cgtttccgcg atcggctcga
     8521 tgcgctcgta gggctcgcgc gcgtacgcga acaggtcctt ggcctcggcg atgtcgccgg
     8581 ccaggaccgc gtcggcgaac tcctgtgtgc gcgcgatcag gatcttcgtc tgtccgagcg
     8641 cgaacgtccg gtaggtctcg acggcggcgt cggcggcttc ggaggtatgg gcgccgacac
     8701 cgcccgacac ggtgagcgtg ccggtgaccg gatcgctgtg gcgcccgcac cgcatctgat
     8761 actcgcccgg ttcgagcgtg aggctgaagg tgcccgtgag tccgggcgcc aggttcgcga
     8821 cctcgcccag gaggagatcg ccatccagga tctcgagctc gggttcgtcg gtgcgatcgg
     8881 tcgtggcctc gaaggtggtg ggtccggctg cggcctccgc ggtggtgggg gaacacccct
     8941 ggtcggtcaa ctgcaccgcc acgacctgga tctgttcgtc ggcgttctcg ccctcacccg
     9001 cctgcgggtc gtcgttcgat ccgcaggccg acaggatcag cgctcctgcc acggccatcg
     9061 ccgccggaac gcggaagcgt cctcgcttca ccatcggcac gtcgtcgccc cccatgtgtt
     9121 cggtctgcct aacagtggtt gtccaccact ctgaacgggc gaatcctatc gcagaggtcg
     9181 gattcactgt caacccagac cacggcgatg ccaggaccca ccgctcagac aggtcgacga
     9241 gctggcgtga tcgacggtca ggcgggaggc cggacgaggg tgggacccct cgggaagaag
     9301 gttcgtcccc cccggtgctg cttgccgacg agctcgttgg tgaagaccca ctcctgcatg
     9361 gggagctcgc ggagttcctc gacgacgagg aacgactgcg gaccgaggcg atcctcgtcc
     9421 tcgtcctcgt cttcgtcctc ctcgtcctcg ttcacctctg cgggttcgtc cggcctcgcg
     9481 agcgcatcgg cctcgcgcgt ttcgacccac gtggccggca gcatcgtggg gagtggatcg
     9541 ccgggttcgt agtgcacgcg gccgacgaac cgggccgccc acgtggcggc ggacacctcg
     9601 tcctcaccgg catccgacgc catgagcagg accgtcgccc cgggcgccgt tcgatccagg
     9661 tcgccgggtc cgccggcggg gagcaccacg cggtcgtgtc gctcgcaggt ctcggcggcg
     9721 gatcgaaggt gcaccagcgg catccctaag agcagggctc gttcatctgc ttggtcggtc
     9781 accgcgctcc cttcccgtcg ccgccatcgt acgtggcttg accgcctgct tgctctttcg
     9841 tagattgtcg acagttcaca tggagccccc ccgcactgga cggtcgcgag gtggctcccc
     9901 gatcaggggg tggcacgtga cggatcgtca ggacgaactg gacgtctacg cagacgggca
     9961 gggcaggcga ggccaggtcg atcgccggag cttcatcagg gccggcgtgc tgggcaccgc
    10021 gatggcgagc tcggtgttga tgacgagcga cctcgcggcc ggcgccgcga tgttgaagcg
    10081 cagcacccgg acggcgacgc ccaggggttc gggcctcgtg caggccggcc ctgaactcgc
    10141 gctgccgccg gggttcagct atcacacgtt cggcgcgttc ggttccgcga tgagcgacgg
    10201 gttcatcacg ccgccgatcc acgacggcat ggcggcgttc gacgtgggcg gcggcaagct
    10261 ccgcatcgtg cgcaaccacg agctcggcga gggcaacgac atcccgccgg gcaccgtgat
    10321 cggcgacccg gccaccgcgt gggacaagcg tgcccccggc ggcaccacca cgatcgaggt
    10381 cgacgccacg accgcggagt acctcggatc gtggatcagc ctgaacggca ccgatacgaa
    10441 ctgcgccggc atgccgaccc cgtggggcac gtggatcacc tgcgaggaga ccacgatcgg
    10501 cgtcgacgcc ggctacaaga agccgcacgg ctacgtgttc gaggtcgatc cgctggccga
    10561 tgagtcggtg ctgaccaagc cctacaagaa gctcggccgg ttcctgcatg aagccggtgc
    10621 ggtcgacccg gacaccgggg tcgtgtacat gaccgaggac gaaggccccg acgggttcta
    10681 ccgcttcgtg ccggaaacct tgggcgacct cacgtcggga cagctccaga tgcttcgtgt
    10741 gaagggtcag ccgaaataca acacgatcgt cggccagacg gtaggggccg tgctcgtctg
    10801 caactgggtc aacatcaaca cccccgaccc ctccgacgcc gaggacaacg cctcggcggt
    10861 cttccacgag ggtcgttcga agggcggcgc caagttcctg ggcggcgagg gttgcaccta
    10921 ccgcgacggc ggagtcgtct tcggatcgtc cgacggcggt gacaagggcc tgggccagat
    10981 ctggcagtac acgccgacca acaacgtcgg tgagctgaac gaacagggcg aactcgtgct
    11041 cctgttcgag tcgggcgggc gtcagacgct cgatggcccg gacaacatga cgaccagtcc
    11101 cgggggcgcg atcgtgatcg ccgaggacgg caacctcaag agcaacttcg tgcgaggtct
    11161 gctgcccaac ggcacgatgt tcccgatcgc cgagaacctg gttgcggtgc agcgtcacta
    11221 catcgaggcc tcgggcaagg tctacgaccc gacggttccc gacgacgggc cttcggcagg
    11281 cgacggtacc ggcttctcgg agttcgccgg tccgacgttc tcgccggacg gcgagtggtt
    11341 gttcgtcaac atccaggttc ccggcatcac ctgtgccatc accggggact gggctagcct
    11401 cggcctgtga gtcgtgacga tggatcgaac ggcgcggtgc cgaagatcgt caccacgccg
    11461 cccggtccga agacgcgtgc catcctcgag cgccagtccg cggtcctgta ccgcgggctg
    11521 gccgaagagg tcggaccgtt cgtcatctgc cgcaagtcgg gccatgagat cgaggacgtc
    11581 gacggcaacg tctacctcga tttcatcagc gcgatggcct cggtgcccgc gggggccgct
    11641 cgcgctgaca tcacggacgc agcggtcgac gcgctgcgtc gctacggcaa cgaggacacg
    11701 cacttctact cgaacgagta cgtgctgccc ttggctgagc gtctcgtggc catcgcgccc
    11761 ggggcgctca gccgtgtcga catcgcgctg aacggcacgg aggccgtcga gacctcggtg
    11821 cggttcatgc gccgcacgac cggccgcccg atcgtgatcg ggttcatggg cggctaccac
    11881 ggggaggcgg ggaccgccgg cgcgatcggc gccgaatcga gcgacctgtc gttcgggtat
    11941 cgcgcgttga tgcccgggtt cgtgcacgtg ccctacccga acccgtaccg cacgccgttc
    12001 acggcgcgcg cgggcggcac gggtgacggc acgatcgact acctgcgcga ccatgtgctg
    12061 caccacgccg tcgatcctcg ggaggtggcc gggttcgtga tcgaacccgt catgggctcc
    12121 ggggggtgcg tggcgccgcc ggacgggttc tggtcggcgc tcaccgatct gtgccgcgag
    12181 ttcgacttcc tgttgtgcgc cgatgaggtg aagacgggat tcggtcgcac gggccacatg
    12241 ttcgcggtgg aacgctgggg cgtggagccc gacctgatgg cgttgggcaa gtcgatgggt
    12301 ggcggcgtga tgccgatcgg cgcggtgctc ggcaccgagc gctcgatgga cttcgacgac
    12361 gtctccacgg gctccacgtg gtcgtggctg cccgccgcct gtgccgcggc gctcgagacc
    12421 ctcgacgtgt tcgaacgcga gccggtgctg gagaacgttc gggtgttgga ggccgcgggc
    12481 ctgcaggcgc tcgaagggct gatggatcgc tatccggcca tcggtgacgt ccgcgcggtc
    12541 ggatgcttcc tggccgtgga gttcgtgaag gaccgggcga cgaaggagcg cgacctcgac
    12601 ctgcaggagc gcgtggcgtg gggatgcctg cgccgtggcc tgctggtgga cccgtccacg
    12661 acctcgctca acctgcagcc ctcgctcacg atgcccgtcg aggtcatggg caccgcgatg
    12721 gccatcctcg acgaggcgat cgccgaagcg gtcgtggcgt gacgtccgtg tccaccgggc
    12781 acgtggacga cctcgcggcg tccgtgcggc gcgacctcgt ggaactcgtg gagatcccct
    12841 cggtctcggg tgaggagcac gccgtggtcg accacctgga acgccggtgc gccgagctcg
    12901 gtctgccgtg tcgccggatg ccggtcgacg gcggggccga cgacctgttg atcggctggc
    12961 acgaacgacc ctccttgctg atcacggcgc acatggacac gatccgtccg acatgggagt
    13021 ggtcgggtgt ggccgaggtg cgagacgggc gcgtcatcgg gctgggcgcc caggacgaca
    13081 aagcctgcgt ggtcgcggcg ctccatgggt tgctcatggc ccgggaggcc ggggtcccgc
    13141 tggagacgct gccggtaggg atcggcctgt gcgtcgacga ggagatcggc ggcacaggct
    13201 cgatctcgat ggcgcgaacg ctccggccgc ccttcgtcgt ggggctggag ggcaccgagc
    13261 tgcagctggg caccgccgag gccgggttcg tcgaggtgtg gtgtcatctg cgcggggtcg
    13321 ccgtgcacgg ggcgctccgc gagctcggcg agaacgcgat cgacaccgcg cacgcgttga
    13381 tcggacgcat ccaggagcat cccggatcgc agcacgaaca cccgttgctg gggcggaaca
    13441 tccccatgat ctgggagatc cgtggagggc agcgcctcaa cgtggtgccc gaccgatgct
    13501 ccttccacct ggactggcgc gtcacgccgg ggggaccgtc cgccgcgtcg ctcctggcct
    13561 ggctcgagga ggcgacggcc gacgccggcg gtgacgtcga actcgtcgag gtcatcgaac
    13621 cgttcgagac tgatcccggc gccgaggtcg tgacggcgct cggcaacgcg gtcgaggctg
    13681 cgaccggcaa gaggcccgct cccaccggca tggtggcctg gaccgatgcg cacaacttcg
    13741 tggatctcgg tggctcgcag gcggtcgtgt tcggccc
//
LOCUS       DATDBZ010000592         3482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1839574, whole genome shotgun sequence.
ACCESSION   DATDBZ010000592 DATDBZ010000000
VERSION     DATDBZ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839574"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VIX62_13730"
     CDS             complement(<1..602)
                     /locus_tag="VIX62_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009719965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY5861336.1"
                     /translation="MSPSKKASPKTPAPSAADSHDLIRVQGARVNNLKDISVEIPKRR
                     LTVFTGISGSGKSSLVFGTIAAESQRLINETYSTFVQGFMPTLSRPEVDVLDGLTTAI
                     IVDQERMGANARSTVGTATDANAMLRILFSRLGKPHIGSPNAYSFNVPSVKASGAITV
                     ERGAGKTQTKKATFTRLGGMCPRCEGMGAVTDFDLTALYD"
     gene            complement(599..1087)
                     /locus_tag="VIX62_13735"
     CDS             complement(599..1087)
                     /locus_tag="VIX62_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY5861337.1"
                     /translation="MSPAKKTTQKSTKSKSKATTAKSSGAFTADERAAMKARAQEQKA
                     EARANKNRADGERALLEAIAGMKGRDRAMAKRVHAIVTASAPELWPKTWYGMPAYARD
                     GKVVCFFQDAAKFNARYATFGFNDSANLDDGAMWPVAYALKELTAAEEKKIAALVKKA
                     AS"
     gene            complement(1084..1578)
                     /locus_tag="VIX62_13740"
     CDS             complement(1084..1578)
                     /locus_tag="VIX62_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018170727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5861338.1"
                     /translation="MTRNPTAAQHDPGVRDRETRTADSLAMLTTQGIDVWVATASAAG
                     APFLVPVSLAWVNDRAVIAVAKSSLTATNLTTSGVARLAVGPTRDVVMIDAVLEEVVD
                     VETDEALGSAYIAQADWDPRDDTGYAFLVLRPVRVQAWREANEIPGRALMRDGAWLTE
                     MEAE"
     gene            complement(1653..>3482)
                     /gene="lnt"
                     /locus_tag="VIX62_13745"
     CDS             complement(1653..>3482)
                     /gene="lnt"
                     /locus_tag="VIX62_13745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY5861339.1"
                     /translation="GWGSLGVSQVDNRLTVRLASVAGVWGITFVVVLVNALIVELAGG
                     AGGRRRWLVGIVAIVAVVAGPVLIPFAGADGEPLDVAVVQVDVREARAATAGEEDVRV
                     AELNIEQHERLIDDPPDLVVWGEGALDPDAAADPETVAAVQDAVASVGSPTIVGAVLN
                     DPDHSQHTSALLLDGSGQVVDRYDKVHLVPFGEYVPFRSRLSFIDAIDQVPVDRVPGK
                     EVHPLSVEGLPPIGAPICFENSFPSIPREMVRQGAGFLVVTVNNASYGTTAASAQHLQ
                     FSQLRAIEVGRWTINGAVSGISAFIDPTGRVVASEGLFEPAILRDTIRSSTQRTWYVR
                     LGDWVPWLLLAFLVIVFALPRRRASKRPDPEPLSEGYRTLVILPTYEERATIEQVLIG
                     VRKAPQDVDVFVVDDSSPDGTGQVVAEIATSDPHVRLLERPAKSGLASAYLEGFRVGL
                     AEGYDVIVEMDSDLSHDPTELPRLLETAAAGKDLVVGSRYIPGGSVTNWSRLRVALSR
                     AGNLYARIMLSLPVHDATSGYRAYRRRLLEDLVAQPIASDGYGFQIELVMRSWDLGYV
                     LGEAPITFREREHGQSKLSRRIVVEALWLVTIWGFQRRLRTPE"
BASE COUNT          529 a         1207 c         1165 g          581 t
ORIGIN      
        1 tcgtcgtaca gggccgtcag gtcgaaatcg gtgaccgcac ccatcccctc gcagcgtggg
       61 cacatgccgc cgagacgcgt gaacgtcgcc ttcttggtct gggtcttgcc ggcgccgcgc
      121 tcgaccgtga tggcgccgct cgccttcacc gacgggacgt tgaacgagta ggcgttgggt
      181 gagccgatgt ggggcttccc gagcctgctg aacaggatgc gcagcatggc gttggcgtcg
      241 gtcgcggtgc ccacggtgga gcgggcgttg gcgcccatcc gctcctgatc gacgatgatc
      301 gccgtcgtca ggccgtcgag aacgtcgacc tcgggccgtg acagcgtcgg catgaagcct
      361 tgcacgaacg tgctgtaggt ctcgttgatc agccgctgtg actccgcggc gatcgtgccg
      421 aacacgagtg agctcttgcc cgaacccgag atgccggtga acaccgtcag ccggcgcttc
      481 gggatttcga cgctgatgtc cttgaggttg ttcacccgtg cgccctgcac gcggatcagg
      541 tcgtggctgt cggcggcgct gggcgcaggt gtcttcggcg acgccttctt cgacggactc
      601 agctcgctgc cttcttgacg agcgccgcga tcttcttctc ttcggcggcc gtcagctcct
      661 tgagtgcgta ggcgaccggc cacatggcgc cgtcgtccag gttcgccgag tcattgaaac
      721 cgaacgtcgc atacctcgcg ttgaacttcg ccgcgtcctg gaagaagcag acgaccttgc
      781 cgtccctggc gtatgcaggc atgccgtacc aggtcttggg ccagagctca ggggcggacg
      841 ccgtgacgat cgcgtggacc cgcttcgcca tggcccgatc gcgtcccttc atgccggcga
      901 tcgcctcgag cagggcacgt tccccatccg ccctgttctt gttcgcacgc gcctctgcct
      961 tctgctcctg ggcgcgggcc ttcatcgcgg cacgttcgtc ggccgtgaac gccccggatg
     1021 acttggcggt cgtggccttg ctcttgctct tggtcgactt ctgcgtggtc ttcttcgccg
     1081 ggctcattct gcctccatct ctgtcagcca ggccccgtca cgcatgagcg cgcgcccggg
     1141 gatctcgttt gcctctcgcc acgcctgcac ccgcaccggt cgcaggacga ggaacgcgta
     1201 tcccgtgtcg tcacgagggt cccaatcggc ttgcgcgatg taggccgacc cgagcgcctc
     1261 gtcggtctcc acgtcgacga cctcctcgag cacggcgtcg atcatcacca cgtcacgggt
     1321 cggtccgacg gcgagccgag ctacgccgga ggtcgtcagg ttggtcgcgg tgagtgagga
     1381 cttggcgacc gcgatcaccg cccgatcgtt cacccaggcc agcgatacgg ggacgaggaa
     1441 cggggctcct gcggccgacg ccgtggccac ccacacgtcg atcccctggg tcgtcagcat
     1501 cgcgaggctg tccgcggtgc gggtctcgcg atcgcggacg cccggatcgt gttgcgccgc
     1561 ggtggggttc ctggtcatct gaggcatcgt gccaggtcga gcccgacgtg ccaaacgtct
     1621 ccgccggtcg gcccgtgctg cgtcggttcg cgtcactcgg gcgtgcgaag gcgacgctgg
     1681 aagccccaga tcgtgaccag ccacagggcc tcgacgacga tccgacggga gagcttcgac
     1741 tgtccgtgct cacgctcgcg gaacgtgatg ggcgcctcgc ccaggacgta gccgagatcc
     1801 caggagcgca tcacgagttc gatctggaac ccgtatccat cagaagcgat cggttgggcc
     1861 accaggtcct cgagcaggcg cctccggtag gcacggtagc cactggtggc gtcgtggacc
     1921 ggcaggctca gcatgatgcg cgcatagagg ttgccggcgc gcgacaacgc cacgcggagc
     1981 cggctccagt tggtcaccga accgccgggg atgtaccggc tgccgaccac caggtccttg
     2041 cctgcagcgg cggtctccag gagccgcggg agctccgtgg gatcgtggga caggtccgag
     2101 tccatctcga cgatgacgtc gtagccctcg gccagaccga cgcggaaccc ttccaggtag
     2161 gcgctggcga gccccgattt ggccggtcgc tccaggaggc ggacgtgcgg atcggacgtg
     2221 gcgatctcgg ccaccacctg ccccgtgccg tcgggtgatg agtcgtcgac cacgaacacg
     2281 tcgacgtcct gcggggcctt gcggacgccg atcaacacct gctcgatcgt ggcgcgctcc
     2341 tcgtaggtgg gcaggatgac gagggttcgg tagccctcgg acagcggttc aggatcgggg
     2401 cgcttcgacg cgcgacgccg cgggagcgcg aacacgatga ccaggaacgc cagcaacagc
     2461 caggggaccc agtcccccag ccggacgtac cacgttcgct gggtggagga ccggatggtg
     2521 tccctgagga tagcgggctc gaacaggccc tcggaggcca ccacgcggcc ggtggggtcg
     2581 atgaaggcac tgatcccgga caccgcgccg ttgatcgtcc atcgcccgac ttcgatcgcc
     2641 cgcaactgac tgaactgcag atgctgagcg ctggccgccg tcgtcccgta cgaggcgttg
     2701 ttcaccgtca ccaccaggaa ccctgcgccc tggcgcacca tctcccgggg gatcgacggg
     2761 aaggagttct cgaagcagat cggcgcgccg atgggcggca gaccctcgac cgacagtggg
     2821 tgaacctcct tccccggcac ccgatccacc gggacttggt cgatcgcatc gatgaatgag
     2881 agccgtgaac ggaaggggac gtattcgccg aacgggacga gatggacctt gtcgtagcga
     2941 tccaccacct gaccgctccc gtcgaggagc agcgcgctgg tgtgctggct gtggtccgga
     3001 tcgttcagca cggcaccgac gatcgtcggg gaccccacgg aggccaccgc gtcctgcacg
     3061 gcggcgacgg tctcggggtc ggcggcggca tccggatcga gcgcgccctc cccccacacg
     3121 accaggtcgg gcggatcgtc gatcaggcgt tcgtgctgct cgatgttgag ctcggcgacc
     3181 cggacgtcct cctcgcccgc cgtggcagcg cgcgcctcgc gcacgtccac ctggaccacc
     3241 gccacgtcca gtggctcgcc gtcggcgccg gcgaacggga tgagcaccgg gcccgccacc
     3301 accgcgacga tcgcgacgat gccgacgagc catcgccgcc gaccgccggc tccgcccgcc
     3361 agctccacga tcagggcgtt gaccagcacg accacgaagg tgatgcccca taccccggcc
     3421 accgaggcga gacgcaccgt gagccggttg tctacctgcg aaaccccgag gctcccccat
     3481 cc
//
LOCUS       DATDBZ010000593         4225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1425632, whole genome shotgun sequence.
ACCESSION   DATDBZ010000593 DATDBZ010000000
VERSION     DATDBZ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425632"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="VIX62_13750"
     CDS             <1..1634
                     /locus_tag="VIX62_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5861340.1"
                     /translation="AVSAGSDWSVVTIQYDMLLKIASKDLGPAPSLATGCEPNEDSTE
                     WTCTLRDDLKWSDGTALTSRDVAFTYRFVIDNKIPQYRSYFSSDPTFETPNDTTLIWK
                     SAEPTFAPDMPPWVYIVPEKVWSQYDGKGLRTIKSVENTPSIASGPFTLTDWNQGQGW
                     TMERNPYFWGEQPALDRIEFRLYSNQEAMIQALRNGEIDMADGLKPSLIASIDGIDDI
                     TVQHVVSDWWLNLAFNFGGQGPDADALPALHDHVVREAIAMAIDKQAISDKVYQGTAT
                     PGDTIIRPASAYWHLDIPAEDEIPFDIPVANDLLEQAGYADTDGDGIREDPQTGEPLQ
                     MLIPASQDTTGAVEAGELIVGYLQQIGIDVDLRPVTDAKMNDYWGAGNFDAYIWYWSG
                     DPDPNYQLFVFTSDQCGSWSDGCWKDPEFDALYDEQRNVFDRADRQAVVFEAQQRAYD
                     QIPGVVLAYPGWLQAYRNDRFTGWVPAPGANGYLLPGYNYDSLIELRPMNDSVAGGSG
                     SGGVPGWLWLAVIGVIAVAGVALARRGRRRELDEA"
     gene            1674..2639
                     /locus_tag="VIX62_13755"
     CDS             1674..2639
                     /locus_tag="VIX62_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5861341.1"
                     /translation="MGTRKYVLRKVLQALITLGFVLTINFFMFRVLPGDPVGLLAKSQ
                     RLTEQDVAELRSELGLDQPLATQYVTYVRQTLTGNLGVSLRTARPVTDVVSSRLGPTL
                     LLVGLGTLLSTVIGIWIGIKGGWRRGSTFDTSTLMGSLALYSIPEGWLGMLLLLLFSG
                     FLGWFPAGGYESGGLTGTARFVDILDHLFLPLLTLTLGYVGQYAIIMRSSMIDVVNED
                     YITSARAKGVPEHLVRTRHAVPNAFLPTFTLIFLNLGFVLGGAVVVEAVFSWPGIGLL
                     TYDAINTLDYPVIQAAFLLASVAVLVCNLVADITYGYLDPRIREA"
     gene            2641..3558
                     /locus_tag="VIX62_13760"
     CDS             2641..3558
                     /locus_tag="VIX62_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5861342.1"
                     /translation="MATEGPVELRSARSLIWARRRATLATHWRTFRRSRQGMIGLTVL
                     LVFIGFAIVGPMVIDPASVEPATASGPITAPPSLAYPLGTDNFGRSVLSLVIVGARIS
                     LLVGFTAALGTMLIGAAVGISAGYFGGTKIDTALNAFTNWFLVLPWIALAIALTAVIG
                     PTLVNVIVVIAITSWASTARVVRSQSLSIKERPFLERSRALGAGHWHLLSHHMLPNVF
                     PILFSSAVLMVALAILSETTLSILGLGPAGAITWGQTIKASFTAGAMSAGWWWWLIPP
                     GLAIMMVTLSFTMVGFALDEVLSPRLRER"
     gene            3598..>4225
                     /locus_tag="VIX62_13765"
     CDS             3598..>4225
                     /locus_tag="VIX62_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861343.1"
                     /translation="MGWLFWAGAAVLAVVAIARLARRRSRQRELMLLCHRAGVSFMPI
                     DPFPDTLWLPFGWLGLGRWIAAENVVWNGADGEAARAFDLITEERPAGEDAQPTQHRF
                     TCASVALPFGCPRLQIEPRDALGGVIDVLTGEDIALELEAFNRRFHVRGSDRRFAVAF
                     CDQRMMQALLGLPPGVSIAVNEDRMLLRSTTLPPAQVLLLLEAARAIAR"
BASE COUNT          650 a         1386 c         1439 g          750 t
ORIGIN      
        1 gggccgtgag cgccggcagc gactggtcgg tcgtcacgat ccagtacgac atgttgctga
       61 aaatcgcatc caaggatctg ggcccggcgc cgagcttggc gaccgggtgc gagccgaacg
      121 aggactccac cgagtggacc tgcacgttgc gcgacgacct caagtggagc gatggcacgg
      181 cgctcacctc ccgcgacgtc gccttcacct accgcttcgt gatcgacaac aagatccccc
      241 agtaccgcag ctacttctcg tccgacccca ccttcgaaac gcccaacgac acgaccttga
      301 tctggaagtc ggccgagccc acgttcgcgc ccgacatgcc gccctgggtc tacatcgtgc
      361 ccgagaaggt gtggtcgcag tacgacggca agggccttcg cacgatcaag agcgtcgaga
      421 acacaccgtc gatcgcgagc ggtccgttca ccttgaccga ctggaaccag ggccagggct
      481 ggacgatgga acgcaacccg tacttctggg gcgagcagcc ggcgctcgat cgcatcgagt
      541 tccgcctcta ttcgaaccag gaggcgatga tccaggcgct gcgcaacggg gagatcgaca
      601 tggccgacgg gttgaagccg tcgctcatcg cctcgatcga cggcatcgac gacatcacgg
      661 tgcagcacgt ggtctcggac tggtggctga acctggcgtt caacttcggt gggcaggggc
      721 cggacgccga cgcgttgccc gccctgcacg atcacgtggt gcgcgaggcc atcgcgatgg
      781 cgatcgacaa gcaggccatc tcggacaagg tgtatcaagg caccgcgacg ccgggcgaca
      841 cgatcatccg cccggcctcg gcgtactggc atctcgatat cccggccgag gacgagatcc
      901 cgttcgacat cccggtcgcg aacgatttgc tggagcaagc cggctacgcc gacaccgacg
      961 gcgacggtat ccgcgaggac ccccagacgg gtgaacccct gcagatgttg atccccgcct
     1021 cgcaggacac gaccggcgca gtcgaggccg gcgaactgat cgtggggtat ctgcagcaga
     1081 tcgggatcga cgtcgatctg cggccggtca ccgacgccaa gatgaacgac tactggggcg
     1141 cggggaactt cgacgcctac atctggtact ggagcggaga ccccgacccc aactatcagc
     1201 tcttcgtgtt cacctccgat caatgtggct cctggagcga cggttgctgg aaagacccgg
     1261 agttcgatgc gctctacgac gaacagcgca acgtgttcga ccgagccgat cgccaagccg
     1321 tggtgttcga ggcgcagcag cgcgcctatg accagatccc gggcgtggtg ctggcctatc
     1381 ccgggtggct ccaggcctac cgcaatgacc ggttcaccgg atgggtgccg gctccgggcg
     1441 ccaacggcta tctgttgccg ggctacaact acgactcgct gatcgagttg cggccgatga
     1501 acgactcggt ggccggaggc tcgggatcgg gcggggtgcc gggctggctc tggcttgcgg
     1561 tgatcggtgt gatcgcggtg gccggggtcg ccttggcgcg tcgcggacgt cgtcgcgagc
     1621 tcgacgaggc gtaggcgtca ccgagacggc gagcgcgcgg aaggggacga ggggtgggca
     1681 cacggaagta cgtgctccgc aaggtgctcc aggcgctcat cacgctcggc ttcgtgctca
     1741 cgatcaactt cttcatgttc cgcgtgttgc ccggtgatcc ggtggggctg ctcgccaaga
     1801 gccagcggct gaccgagcag gacgtcgcag aactccggag cgagctgggg ctggatcaac
     1861 cgctggctac ccagtacgtc acctacgttc gacagaccct gaccggcaac ctgggagtgt
     1921 cgcttcgtac cgcgcgtccc gtcaccgatg tggtgtcgtc ccgtttgggt cccaccctgt
     1981 tgttggtcgg tctcggcacc ctgctctcga cggtgatcgg gatctggatc gggatcaagg
     2041 ggggctggcg tcgcggcagc accttcgaca cctcgaccct gatgggttct ctcgcgctgt
     2101 actcgatccc cgagggctgg ctcggcatgt tgctcctgtt gctgttctcg gggttcctcg
     2161 ggtggttccc cgccggcgga tatgaatcgg gcggcctcac ggggacagcc cggttcgtcg
     2221 acatcctcga ccacctattc ctgccgctgc tcaccctgac gctgggctac gtggggcagt
     2281 acgcgatcat catgcgttcc tcgatgatcg acgtggtcaa cgaggactac atcaccagtg
     2341 cgcgagccaa gggagtgccc gaacatctgg tgaggactcg gcacgctgta ccgaacgcgt
     2401 tcctcccgac gttcacgctg atcttcctca acctggggtt cgtgctgggc ggcgcggtgg
     2461 tggtcgaggc ggtgttcagc tggccgggga tcggtctgct cacctacgac gcgatcaaca
     2521 cactcgacta ccccgtgatc caggcagcgt tcctgttggc gagcgtggcg gtgctggtgt
     2581 gcaacctggt ggccgacatc acgtacggct acctcgaccc gcggatccgg gaggcctgag
     2641 atggcgaccg aaggtcccgt cgagcttcga tcggcccggt cgctgatctg ggcgcgacgc
     2701 cgcgccaccc tcgcgaccca ttggcggacg ttccgccgaa gcaggcaggg gatgatcggg
     2761 ctcacggtgc tgctggtctt catcgggttc gcgatcgtgg ggccgatggt gatcgacccg
     2821 gcctcggtcg agcccgcgac cgcgagcggc ccgatcacgg cgcctccgtc gctcgcctac
     2881 ccgctcggga ccgacaactt cggccgctcg gtgctgtccc tggtgatcgt gggcgcgagg
     2941 atctcgctgc tggttgggtt cacggcagcg ctcggcacga tgttgatcgg cgccgcggtg
     3001 gggatctcgg cgggctactt cggtggcacc aagatcgaca ccgcgctcaa cgccttcacg
     3061 aactggttcc tcgtcctgcc gtggatcgcg ctggcgatcg cgctgaccgc cgtgatcgga
     3121 cccacgctgg tcaacgtcat cgtggtgatc gcgatcacct cgtgggccag caccgcccgc
     3181 gtcgtcaggt cgcagtcact gtcgatcaag gagcggccgt tcctggaacg atcccgtgcc
     3241 ctcggagcgg gtcattggca cctgctgtcc catcacatgc tgcccaacgt cttcccgatc
     3301 ctgttctcga gcgccgtgct gatggtggcg ctggcgatcc tgtcggagac cacgctgtcg
     3361 atcctgggcc tggggccggc gggcgcgatc acgtggggtc aaacgatcaa ggcatcgttc
     3421 acggccggcg cgatgtcggc cggctggtgg tggtggctga tcccgccggg cttggcgatc
     3481 atgatggtca cgctgtcgtt caccatggtg gggttcgcgc tcgacgaggt gctgagccct
     3541 cgattgcgcg aacgctgacg ggtcaagcgg ggccccgagg ctgccgacgc agccaccatg
     3601 ggatggctct tctgggccgg cgccgccgtg ctggccgtgg tggcgatcgc tcgactggcc
     3661 aggcgccggt cgcggcagcg cgaactcatg ctgctgtgcc accgcgccgg ggtgtcgttc
     3721 atgccgatcg atccgttccc cgacacgctg tggcttccgt tcgggtggct gggcctcggc
     3781 cggtggatcg cggcggagaa cgtggtgtgg aacggggccg acggtgaggc cgcccgcgcg
     3841 ttcgacctga tcacggaaga gcgccctgcc ggcgaggacg cgcagccgac ccagcatcga
     3901 ttcacctgcg ccagcgtggc cctgcccttc ggatgtccgc gactgcagat cgagccgcgg
     3961 gacgcgctcg gcggcgtgat cgacgtgctc accggcgagg acatcgccct ggagctcgag
     4021 gcgttcaatc ggcggttcca tgtgcgcggc agcgaccgcc ggttcgcggt ggcgttctgc
     4081 gatcagcgga tgatgcaggc gttgctcggc ctgccccccg gggtgagcat cgcggtcaac
     4141 gaggatcgga tgctgctgcg ctccacgacc ctccctcccg cccaggtcct gctgctcttg
     4201 gaggccgcgc gggcgatcgc ccggc
//
LOCUS       DATDBZ010000594         5253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_189823, whole genome shotgun sequence.
ACCESSION   DATDBZ010000594 DATDBZ010000000
VERSION     DATDBZ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189823"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..577
                     /locus_tag="VIX62_13770"
     CDS             113..577
                     /locus_tag="VIX62_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY5861344.1"
                     /translation="MADPVIPVVPDDETLRRILAEARTIAVVGLSSKPGRASLDIGHS
                     LQERGYRIIPVNPRETQVLGETAYPSLRDIPEEVHVDIVDVFRRAEDTPEIARDAVAI
                     GAKAFWLQLDIVSEEAGAIASAGGLDVIMGVCIKQTAQRLDRDQEREGRNDG"
     gene            570..746
                     /locus_tag="VIX62_13775"
     CDS             570..746
                     /locus_tag="VIX62_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007619014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861345.1"
                     /translation="MGEAVTVWEYFVAPLLEHNPGEILDAFGQDGWELVNVVAQPTPA
                     GGSSTVAYLKRPKG"
     gene            759..1298
                     /locus_tag="VIX62_13780"
     CDS             759..1298
                     /locus_tag="VIX62_13780"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEY5861346.1"
                     /translation="MDIETIFGLPAHPLLVHVPVVLIPLCAAGAVWIVVWPSWRPRIG
                     WIVVVLSGFTVVMSKLAADSGEALEDALDRESSLLERHTELGDTFVWFALAFFVAVLA
                     LMLWDRAQRRKADPSGGSTGRARATTPAAIVLSILVVVTAAAASFRVYQVGHSGAESV
                     WNEDQGSSDSSGPGSGEEG"
     gene            complement(1295..2509)
                     /locus_tag="VIX62_13785"
     CDS             complement(1295..2509)
                     /locus_tag="VIX62_13785"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY5861347.1"
                     /translation="MARRSLRMLSLTFAAFAFAAFAAPRSFAAQPGPKPFLQGLAFPT
                     NLAFAPDGRLFYTEKSTGNVRVVHDGTLRQEPFVTLPVVPEAERGLLGIAIGPDFEAD
                     PWIYLYLSDATDGVNRLVRVRADGDRAAGEPETLLEGLDSAAGYHNGGDLAFGVDGSL
                     FVTLGEAHEADRAQDPSDLGGKIVRLEPDGSVPADNPFGAGNPVWSYGHRNSFGICVD
                     PGDGTLWETENGPDVDDEVNRIERGGNYGWPLVTGRSNDDDFVQPVVVFPDTIAVTGC
                     TVVDGDLYFGSFDGRLWRLPGAQQDTGDVEEIARLDAGVTDVALGPDGLLYIATNDAI
                     LRMAPIAAGVQSPSSSVSPTSASPSTSVAAAEGDGNDSTARTWITVAALGALAIGLGL
                     RFAAGRRMRREG"
     gene            2572..3204
                     /locus_tag="VIX62_13790"
     CDS             2572..3204
                     /locus_tag="VIX62_13790"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5861348.1"
                     /translation="MLSIDHAVLAVGDLDEAGERILRTHGLASVPGGVHPRWGTANRI
                     IPLGRDYLELIAVVDPAVADTTAFGRTLLALTADGRDHWAELCVADTDVDATAARLGL
                     EVEAGLRTTPDGREIRWHGAGLEEDVRRPYLPFFITWDVPDDLMPGRMAADHLTPASG
                     IERVEVAGDAAVLDAWLGGAGLPIDVVAGPEGITAVTLSLADGGSLELCP"
     gene            complement(3201..4355)
                     /locus_tag="VIX62_13795"
     CDS             complement(3201..4355)
                     /locus_tag="VIX62_13795"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEY5861349.1"
                     /translation="MSDDTFRFETRAIHAGQDPDAPYGSVNVPIYQTATYVQPAVGEP
                     KVWDYARGGNPTREAFQQALAALEGAERGFAFSSGLGAETTLLLTLRPGDHVVLADDV
                     YGGTYRLLSSVLSSWGLTFTTVDLGDQAALVDALGPMTKLVWIETPSNPLLKIVDIGA
                     VAATAHAAGARVVVDNTFATPALQRPLALGADAVVHSVTKYIGGHSDLIGGAIVTSDE
                     EWIEQLGFLTNAIGAVPGPMDCYLALRGLKTLAIRMRAHCSGARAIAGFLQTHRDVSA
                     VYYPGLSDHAGHAIATRQMADFGGMVSFRVGSAARAVDLVGQTELFFLAESLGGVESL
                     IEVPGPMTHASVAGSPLEVPDDLVRLSVGIEHPDDLIEDLARALDLSGRV"
     gene            complement(4426..>5253)
                     /locus_tag="VIX62_13800"
     CDS             complement(4426..>5253)
                     /locus_tag="VIX62_13800"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEY5861350.1"
                     /translation="GIDVGVGKGLDLVLLDERRVPLQVISHVRVPEVERLIRDLRPDV
                     IAIDSPPKWAASGRSRRTERELASLNIQSFNTPSKEHGERNRFFGWMQVGFEVFAQAA
                     EAGFPTYAAGSPKGKALEVFPHASAVVLAGGLAPKGTKKYAWRTAVLRNQGVRTDELS
                     SPDRVDAALAALTGLLALDGKRFAPGDPTEGVIVVPSASLPATPYRAVARPPKDAQPL
                     FHYCGCGDASCDQLVRGEFAPGHDAKRKAMLWTKAREGVDAVEELATRGWKLPPEMR"
BASE COUNT          774 a         1807 c         1866 g          806 t
ORIGIN      
        1 acggaggcag gacctggaaa gggttcgcct acgagctcga cggcgcgacg atctggggcg
       61 ccacgggctg gatgctccac acgttgctcg agacgatcag gaaggacaca tcgtggccga
      121 tcccgtgatc ccggtggtgc cggacgacga gacgcttcgt cgcatcctcg cggaggcgcg
      181 cacgatcgcg gtggtgggcc tttcgtccaa acctggccgc gcgtcgctcg acatcgggca
      241 ctccctgcag gaacggggct accggatcat cccggtgaac ccgcgtgaga cccaggtgct
      301 cggcgagacg gcctatcctt cgctgcgcga catccccgag gaggtccacg tcgacatcgt
      361 cgacgtgttc cggcgagccg aggacactcc ggagatcgcc cgcgatgcgg tggcgatcgg
      421 cgccaaggcg ttctggctcc agctcgacat cgtgagcgag gaagcgggag ccatcgcctc
      481 ggcgggtgga ttggacgtca tcatgggggt ctgcatcaag cagaccgcgc aacgtttgga
      541 ccgcgaccag gaacgggagg gacgcaacga tgggtgaggc cgtgaccgtc tgggagtact
      601 tcgtggcacc gctgctcgaa cacaaccccg gcgagatctt ggacgcgttc gggcaggacg
      661 gctgggagtt ggtgaacgtc gtggcccagc ccaccccggc gggtgggagt tcgaccgtcg
      721 cctacctcaa gcgtcccaag gggtagcgga gcccgtccgt ggacatcgag accatcttcg
      781 gcctcccggc gcacccgctc ctggtgcacg tccccgtcgt cctgatcccg ctctgtgcgg
      841 cgggcgcggt ctggatcgtc gtgtggccgt cgtggcgacc ccggatcgga tggatcgtcg
      901 tggtcctgtc ggggttcacc gtggtgatgt cgaagttggc cgccgacagt ggggaggcgc
      961 tcgaggatgc cctggacagg gaatcctccc tcctcgagcg tcacaccgaa ctcggcgaca
     1021 cgttcgtctg gttcgccctg gcgttcttcg tcgcggtgct cgcgctgatg ctctgggatc
     1081 gggcgcagcg caggaaggcc gatccctcgg gcggatccac cggccgggct cgcgccacga
     1141 cgccggccgc catcgtgctc agcatcctgg tggtggtgac ggcggcggcg gcatccttcc
     1201 gcgtctatca ggtgggccac tcgggggccg aaagcgtctg gaatgaggac cagggttcgt
     1261 ccgactcatc ggggccgggt tcgggtgagg agggctagcc ttctcggcgc atacgccgtc
     1321 ctgccgcgaa ccgcagcccc agcccgatcg ccagcgcccc gagcgcggcg accgtgatcc
     1381 acgttcgggc agtcgagtcg ttgccgtctc cttcggccgc tgcgacggac gttgatggag
     1441 acgccgaggt cggcgatacg gacgaggacg ggctctgcac tccggcggcg atcggtgcca
     1501 tgcgcaggat cgcatcgttc gtggcgatgt acaggagacc gtcgggcccc agggcgacgt
     1561 cggtgacgcc cgcgtcgagc cgggcgatct cttcgacgtc gcccgtgtcc tgctgtgcgc
     1621 ccggcagccg ccacagccga ccgtcgaacg agccgaagta caggtcgcca tcgaccacgg
     1681 tgcatcccgt caccgcgatc gtgtcgggga agaccacgac cggctgcacg aagtcatcat
     1741 cgttcgaccg gcccgtgacg agcggccagc cgtagttgcc cccgcgctcg atgcggttca
     1801 cctcgtcgtc gacgtcaggt ccgttctcgg tctcccacag cgtgccgtcg cctggatcga
     1861 cgcagatccc gaacgagttg cggtgcccat aggaccagac cgggttgccg gcgccgaacg
     1921 ggttgtccgc ggggacggaa ccgtcgggct cgagccgcac gatcttgccg cccaggtcgc
     1981 tcgggtcctg tgcgcggtcg gcctcgtgcg cctcacccag cgtgacgaac agtgagccgt
     2041 ccaccccgaa cgcgaggtcg cctccgttgt ggtagcccgc cgccgaatcg agtccctcga
     2101 gcagcgtctc gggctcaccg gcggctcggt cgccatcagc acggacgcgc acgagccggt
     2161 tgaccccgtc cgtggcgtcg gacaggtaga gatagatcca cggatcggcc tcgaaatcgg
     2221 gcccgatcgc gatgccgagg agtccgcgtt cggcctcggg gaccacgggc agggtcacga
     2281 acggttcctg acgcagcgtg ccgtcgtgca cgacccgcac gttgccggtc gacttctcgg
     2341 tgtagaagag ccggccgtcc ggcgcgaagg cgaggttcgt gggaaaggcc agcccttgca
     2401 ggaacggctt gggccccggc tgcgcggcga aggacctggg cgccgcgaac gccgcgaacg
     2461 cgaacgccgc gaacgtcagg ctcagcatgc gcaacgatcg gcgggccatg cctccgagcg
     2521 tacggcgtgc atgccacgat ggtggctcga cgacggggga aaggcggacc gatgctctcg
     2581 atcgatcatg cggtgctggc ggtgggcgac ctggacgaag cgggcgagcg gatcctgcgg
     2641 acccacggcc tcgcatcagt gcccggtggt gtccacccgc ggtggggcac cgcaaaccgg
     2701 atcatcccgc tggggcgcga ctacctggag ctgatcgccg tcgtcgatcc ggcggtcgcc
     2761 gacaccaccg cgttcggtcg gaccctcctg gccctcaccg cggacggccg cgaccattgg
     2821 gccgagctct gcgtcgccga caccgacgtc gatgccacgg cggcccgact gggcctcgag
     2881 gtggaggcgg gcctgcgcac cacccccgac ggccgcgaga tccgctggca cggggccggc
     2941 ctcgaggagg acgttcgccg gccgtacctg ccgttcttca tcacgtggga cgtgcccgac
     3001 gacctgatgc ccggacggat ggcggccgac cacctgacgc cggcgagcgg gatcgaacgc
     3061 gtcgaggtcg cgggcgacgc cgcggtcctg gatgcgtggc tcggcggcgc cggcctgccc
     3121 atcgacgtcg tcgccggccc cgagggcatc acggcggtga cgttgtcgct cgccgacggt
     3181 gggtcgctcg agctttgccc ctagacgcgc cccgacaggt cgagggctct cgccaggtcc
     3241 tcgatgaggt cgtccggatg ctcgatgccg accgagagcc gcaccaggtc gtccggcact
     3301 tccaacgggg aaccggcgac gctcgcgtgg gtcatgggcc ccggcacctc gatcagcgac
     3361 tccactccgc cgagggattc ggccaggaag aagagctcgg tctgcccgac gaggtccact
     3421 gcgcgggcag ccgaccccac gcggaacgac accatcccgc cgaagtcggc catctgccgg
     3481 gtcgcgatcg catggcccgc gtggtcggac agccccgggt agtacacggc cgagacgtcg
     3541 cggtgggtct gcaggaaccc ggcgatcgcg cgggcacccg agcagtgcgc ccgcatgcgg
     3601 atcgccaggg tcttcaggcc tcgcagggcg aggtagcagt ccatcggccc gggcaccgcg
     3661 ccgatcgcgt tcgtcaggaa cccgagctgt tcgatccact cctcgtcgct ggtgacgatc
     3721 gccccgccga tcagatcgga gtggccgccg atgtacttcg tgacactgtg caccacggcg
     3781 tcggctccca gagccagcgg tcgctgcagc gccggggtgg cgaacgtgtt gtccaccacc
     3841 acccgcgcgc cggcggcatg ggcggtcgcg gccaccgcac cgatgtcgac gatcttcagc
     3901 agcgggttcg acggggtctc gatccagacg agcttcgtca tcggtccgag cgcgtcgacg
     3961 agcgcggcct gatcaccgag atcgacggtg gtgaaggtca gaccccacga ggagagcacc
     4021 gaggacagca gtcggtaggt accgccgtac acgtcatcgg cgagcacgac gtggtctccg
     4081 ggccgcagcg tcagcagcag cgtggtctcg gcgccgagcc cgctcgagaa cgcgaacccc
     4141 cgctcggcgc cttcgagggc cgccagcgcc tgctggaacg cctcgcgggt ggggttgccg
     4201 ccacgcgcgt agtcccacac cttgggttca ccgaccgccg gctggacgta ggtcgcggtc
     4261 tgatagatcg ggacgttgac cgatccgtac ggggcgtcgg gatcctggcc cgcgtggatc
     4321 gcgcgggtct cgaaccggaa ggtgtcgtcg ctcatgtgct cctcttgggg ctcctcgtgg
     4381 ggcggtcggt cgggcgggcg tacacggtgc ggcgtgtcgg gtcggtcatc gcatctccgg
     4441 tggcagtttc catcctctgg tcgccaactc ctcgacggcg tcgacgccct cgcgggcctt
     4501 cgtccagagc atggccttgc gcttggcgtc gtgcccgggc gcgaattcgc cgcgcaccaa
     4561 ctggtcgcag gaggcatcgc cgcagccgca gtagtggaac aacggttgcg cgtccttggg
     4621 gggccgggcc acggctcggt acggggtggc ggggagcgag gcagagggca ccacgatgac
     4681 gccttcggtc gggtcgcccg gcgcgaaccg cttcccgtcg agcgccagca gccccgtcaa
     4741 ggccgccaac gcggcgtcga ccctgtcggg ggaggacagt tcgtcggtcc gcacgccctg
     4801 gttgcgcagc acggcggttc gccacgcgta cttcttcgtg cccttgggcg ccagccctcc
     4861 agccagcacc accgcgctgg cgtgcgggaa gacctcgagc gccttgccct tcgggctccc
     4921 cgcggcatac gtggggaacc cggcttccgc cgcctgggcg aacacctcga accccacctg
     4981 catccacccg aagaagcggt tgcgttcgcc gtgttccttg ctgggggtgt tgaacgactg
     5041 gatgttgagc gaggccagtt cgcgctcggt ccgtcgggat cgaccggacg cggcccactt
     5101 cggcggggaa tcgatcgcga tgacgtcggg ccgcaggtcg cggatcaggc gctccacttc
     5161 ggggacccga acgtgcgaga tcacctgcag cgggacgcgg cgttcgtcca agagcaccag
     5221 atcgagcccc ttgccgaccc cgacgtcgat ccc
//
LOCUS       DATDBZ010000595         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1840769, whole genome shotgun sequence.
ACCESSION   DATDBZ010000595 DATDBZ010000000
VERSION     DATDBZ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840769"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VIX62_13805"
     CDS             <1..553
                     /locus_tag="VIX62_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5861351.1"
                     /translation="ELIELPADAVRELTARRFSGDDGIGAMVRPYLAQLVQQSDGLDG
                     HLARRLARSAVELVGTLLEEHAADLPYTGGHGTIMRRVLDYIDSHLSSPDLCPAEIAS
                     AHFISVRHLHALFSTHSTSVSTTIRTRRLERTYDELVNPAYSDRSVTMIALANGFVDA
                     AHFSRTFRAHYGVPPSALRPTTR"
     gene            683..2098
                     /locus_tag="VIX62_13810"
     CDS             683..2098
                     /locus_tag="VIX62_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5861352.1"
                     /translation="MSQTTTTGLLETITVPASEGRAIPDAATGETMGYAPVHGLDDLN
                     RVVAAARAAQPAWAAIGHAERSAILRRTADEVEAHAEELARIIAQEQGKPLNGPGARF
                     EAGACAAWIRNAADTALDPEVVFEAPGTRSELHYGPLGVVGAIGPWNWPAMIAIWQIA
                     PSLRMGNTVVSKPSEYTPLSVLAVAELMNRHLPEGVLTVVSGGRDVGEAIAAHPDIDK
                     IMFTGSTKTGREIVKISAGNLARLTLEMGGNDPGIILPGTDVKVIAENLFWGVFINTG
                     QTCASLKRLYVHDSQYEEVVTTLADMAAGMPMGPGLDETSVLGPLSNKQQFDIVSRLV
                     DDARSRGARIVTGGEAAPELGPHFYRATVVADIDDGAPLVDEEQFGPAIPVIRYTDVD
                     DAVARANASDQGLGASVWGNDVAEAVAVAQRIEAGTVWINQHGTLNPDVPFGGVKGSG
                     YGMEFGREGLKAVAAPKVITV"
     gene            2142..>2541
                     /locus_tag="VIX62_13815"
     CDS             2142..>2541
                     /locus_tag="VIX62_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5861353.1"
                     /translation="MTGHYDAIVIGAGSAGSVVTRRLIDAGRRVLLLEAGGPDTNPMI
                     HDMVGMGSLWHGPEDWDYYTVPQAHAADRRLHLPRGKVLGGSHSLNAAIWVRGDAADY
                     DGWEADGCTGWGWKDVLPVYQAIENYDGGPG"
BASE COUNT          435 a          884 c          838 g          384 t
ORIGIN      
        1 ggagctgatc gaactgcccg ccgacgcggt tcgtgagctc accgcgcgac gcttctctgg
       61 cgatgacggc atcggggcga tggtgcggcc ctacctggcg cagctcgtgc agcaatccga
      121 cgggctcgac ggccatctcg cgcggcgact cgcccgctca gccgtcgaac tcgtcggcac
      181 cctgctcgaa gagcatgcgg ccgacctccc gtacaccggc ggccatggga cgataatgcg
      241 ccgggtcctg gattacatcg acagccatct gagctcaccc gacctgtgcc ccgccgagat
      301 cgcctcggcg cacttcatct ccgtgcgcca cctccacgcc ctcttcagca cacacagcac
      361 gtccgtctcg acgacgatcc gcacccgccg tctggagcgg acctacgacg agctcgtcaa
      421 tccggcctat tccgaccggt ctgtgacgat gatcgcgctc gccaacgggt tcgtcgatgc
      481 ggcgcacttc agtcgcacgt tccgcgcgca ctacggcgta ccgccgtccg cgctgcgacc
      541 caccactcgc taggcgcttc ctctccagag acggctcccg ctgaggcgtg cgccgaacga
      601 cagatgccgt gcactcggcg tcaaactgcg ccaccgtgct ccctcccaga ctggtctcag
      661 acctttcatc gaggaggatg acatgagcca gaccaccaca accggcctgt tggagacgat
      721 caccgtgccg gcgagcgagg ggcgcgcaat ccccgacgcc gcaacaggcg agacgatggg
      781 ctacgcgccg gtgcacggcc tagacgacct gaaccgcgtt gtcgccgctg cgcgggccgc
      841 gcagcccgcc tgggctgcga tcggtcacgc tgagcgctcg gcgatcctcc gccgcacggc
      901 cgacgaggtc gaggctcacg ccgaggagtt ggcgcgcatc atcgcgcagg agcagggtaa
      961 gccgctcaac gggcccggag cgcgtttcga ggccggtgcg tgcgctgcat ggattcggaa
     1021 cgccgccgac accgcactcg atcccgaagt ggtcttcgag gctccgggga cacggtccga
     1081 gctgcactac ggtccgctcg gcgtcgtcgg agcgatcggg ccgtggaatt ggccggcgat
     1141 gatcgccatc tggcagatcg ccccgtccct gcgcatgggc aacaccgtcg tcagcaagcc
     1201 ctcggagtac acgcccctca gtgtgctggc cgtcgccgaa ctcatgaacc gccacctgcc
     1261 agagggagtg ctgaccgttg tctcgggagg ccgcgacgtg ggtgaagcga tcgcggcgca
     1321 ccccgacatc gacaagatca tgttcaccgg atcgaccaag accgggcgcg agatcgtgaa
     1381 gatctcggcc ggcaacctcg cacgcctgac gctcgagatg ggcggcaacg acccgggcat
     1441 catcctgccc ggtacggacg tgaaggtcat cgccgagaac ctcttctggg gcgtgttcat
     1501 caacaccggg cagacctgcg catcgctcaa gcggctctac gtgcatgact cccagtacga
     1561 ggaagtcgtc acgacgctcg ccgacatggc cgcaggcatg ccgatgggcc ccggtctcga
     1621 cgagaccagc gtgcttggcc ccctctccaa caagcagcag ttcgatatcg tctcccgcct
     1681 cgtcgacgac gctcggtcac gtggtgctcg catcgtgacg ggcggtgagg ccgccccgga
     1741 gctcgggccc cacttctatc gcgcgaccgt ggtggcggac atcgacgacg gtgcaccgct
     1801 cgtcgacgaa gagcagttcg ggccggcgat cccggtcatc cgctataccg atgtggacga
     1861 tgcggttgct cgcgcgaatg cgtcggacca ggggctcggg gcatccgtct ggggcaacga
     1921 cgtggccgag gccgtcgctg tggcacagcg gatcgaggcg ggcacggtgt ggatcaacca
     1981 gcacggcacg ctgaaccccg acgtgccgtt cggcggggtc aagggctccg gttacggcat
     2041 ggaattcggt cgcgagggct tgaaggccgt cgccgcaccc aaggtcatca cggtctagac
     2101 gccgcgaacc aaccactcag acatccgacg aaggagatgc aatgacgggt cactacgacg
     2161 cgatcgtgat cggagcggga tcggccggct cggtcgtgac ccggcgcctg atcgatgcgg
     2221 ggcgccgcgt gctgctgctc gaagcgggcg gccccgacac gaaccccatg atccacgaca
     2281 tggtcggcat gggctcgctc tggcacgggc ccgaggactg ggactactac accgtgcccc
     2341 aggcgcacgc ggccgatcgc cgactgcacc tcccgcgggg aaaggtgctc ggcgggtcgc
     2401 attcgctcaa cgccgcgatc tgggtgcgcg gggatgccgc cgactacgac ggctgggaag
     2461 cagacggctg caccggatgg ggatggaagg acgtcctacc cgtctatcag gcgatcgaga
     2521 actacgacgg cggaccgggt g
//
LOCUS       DATDBZ010000596         8590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2023994, whole genome shotgun sequence.
ACCESSION   DATDBZ010000596 DATDBZ010000000
VERSION     DATDBZ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023994"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VIX62_13820"
     CDS             <1..760
                     /locus_tag="VIX62_13820"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY5861354.1"
                     /translation="LLAFFRRRDRAAGVALGLGAATKLYPAVLAVPLIAQRLRERRPD
                     GAIVLAWTTAGAWLLVNLPFAAAAPTAWLTFFRFNGERGADFDSLWYIACRHLDLCPS
                     HRAINVASFAIFLISIAVVWAIKVRRHPDFPRWTLGLPLLVAFLLSNKVYSPQYGLWL
                     LPWFALALPRLWPFLAFSLTDVAVFVTRFSWFGRLQGASGSSQALFEVMVLLRAAVLL
                     WCVVSWIRREHEPIEIERRERAASGVLEIERVRS"
     gene            757..1965
                     /locus_tag="VIX62_13825"
     CDS             757..1965
                     /locus_tag="VIX62_13825"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEY5861355.1"
                     /translation="MTDPSPDAFAPATRRTTGGDGEAVRLRDGLRDSLLVFLGVRIGF
                     SVLGLVAVGLIAPRQGPPAVAGWPIAPVTYGWEALFTAAERQDAAWFLTIATRGYDVA
                     DGSAAFFPVYPLSIRLVAGLPGIGPLGAALIVSNVCFAGALAMLHGLTRLEGMPRETA
                     RLTVLFLAIFPTGFFFLAPYTEGPFLLASISAFWFARRDRWWLAALAGALAAGTRSVG
                     VLIAVALCVEAVLQHRRLGRTLAPRIAAALAVGVGPLLYLAWWRVQHGDATVPWTAQR
                     NWSRESRWPWTTVAEGVGDAWRLGSYWLVDALVVGVVLLAVIVGLRWLRPTYSVYAIA
                     SLALPLVYAWPTRPLLSMPRFVAVVFPAFWVLARAAERRRIPPPAIVAVFAGGYAILG
                     ALFVTWWDVF"
     gene            2068..2706
                     /locus_tag="VIX62_13830"
     CDS             2068..2706
                     /locus_tag="VIX62_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5861356.1"
                     /translation="MTIKVAIADDHSLVRQGLRRYLDMADGIEVVGEAADGKELLDLI
                     GTETPDIALIDIRMPEMDGLEAAREIRDRWPEIGVIMLTAYDDRQFVVEAVRSGARGY
                     VLKARDAEHLIQTVRLVAGGNMVIDPQLVVALAEELSTAKERDRKAETLTAREIEVLQ
                     LLAFGHTNRDIAERLYISPDTVKTHLEHIFEKLGASDRTAAVAEALRRRLIE"
     gene            2824..4959
                     /locus_tag="VIX62_13835"
     CDS             2824..4959
                     /locus_tag="VIX62_13835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY5861357.1"
                     /translation="MSDVAVRPRARTATGLFLGAAAVAAVVTYLAFPSGSREQGLAAQ
                     VMVAGSCLLSGGLILRAVGKGEAWRGTVHLAYALFMLGAVNVVFLAVDLNDGGTYEPR
                     VTDLAFALFLVPLAQLARAEFHAHFDARDGIEIGTDIFLITASVTALMYVVVRPFDAS
                     LQASITAAVFSAVAAFQVAAFGALALWAPSRRHLVLFGIFAVTAAAAATFGWHWTRGS
                     FDSTIASIVLPFMLCPLAFAAWTVFTGIPDSATGSTASPRRWARPVLTSLSVVTACAA
                     LAVVAIFDEERGIDGAQSTAIILLLGLGIAARILSNQTASTIAHRQTAEALGERERAL
                     AETDQALERVREANETLRQSEEHLRLVFETAVDGIVELDERGVVLRANEAFAEMIALP
                     RESIEDQPWTALASVVVGADDAFAGLRTGGQSQITRPGGQVLHLESRTSAVPATPPRT
                     LLLVRDVTAARVADQTIRSLFQFLQDRDEDRSRLMRRTNAAIEAERNRIARDLHDGPV
                     QGVSAASLSLEAALLMIQAGDVDQGVEVLTKIREELAGEADALRGLMSGLRPPVLEER
                     GLLPALRETLVRFGADQGVDTEFAGRVDVPLPEDLETLAYRVVQEALSNAAKHAGATQ
                     VMVMVQANDSQLRVEVEDDGQGFDAGLARDFLREGRVGLASMRERVELASGSFVVRSS
                     PGRGTSIVATLPLDELAPSRRGTTLHEAS"
     gene            complement(4962..7166)
                     /locus_tag="VIX62_13840"
     CDS             complement(4962..7166)
                     /locus_tag="VIX62_13840"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY5861358.1"
                     /translation="MSTTTPTRPEGAKHVPKHAPSTRKKGFFRRFWWVFVLVPLLVVV
                     AIGGSLYMAYARISLPETLPPIRTSYLYDRNGEVLTPLHGTVDRRIVGLKQISDNLEH
                     AVLATEDAGFYDHPGIDVRGIVSAAWTDLVKRETVVGASTITQQLVKNVYAGEYIEHP
                     NGAQEYIVPPRTIKEKIREALLAIKVEQQLTKDQILAKYLNTVYFGHGAYGAEAAALT
                     YFDKPASELTVNEAAVLAAVLHAPSLYDPIKSKYDNEFRRDYTLDQMAKYGYIPPSEA
                     ARLKELKCCGIPKSQKNEDARISSPAGSEYFVEWTRSELFQRYGSARVYGGGLQITTS
                     LDLRLQRMAEDAVEANLPDTDYNPDAALVSIDNETGEVLAMVGGRDWNASKLNLATLP
                     CEGCGRQAGSAFKPFTLAAALDQGFALRGQYWSGPSTMTIADPQCSGPDGLWTPTNAG
                     DSSAGTVDLYGATVNSVNTVYAQLVSALAGGPADVVDMAHDLGIQSELPAVCSITLGS
                     VQINPLEMTNAYATLAADGVYHRANPLLAVDRPNGQPADRRVASRPKDPFKGDTDVPR
                     AVTSALQGVVQGGTGSAASIYPWPAAGKTGSAQDNVDAWFCGYTVQLSTCVWVGYPEE
                     QRPLINVAGVPTVYGGTIPAAIWHDYMLEAMAYGAEEWGWEPLPFSTDFSLTGTLGAP
                     TPIASPTPSASPSPEPSETPEPTVEPSETPPPSETPPPSETPPPSETPPPDE"
     gene            complement(7270..7692)
                     /locus_tag="VIX62_13845"
     CDS             complement(7270..7692)
                     /locus_tag="VIX62_13845"
                     /inference="COORDINATES: protein motif:HMM:NF036456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEY5861359.1"
                     /translation="MPKQQGNRPRRTPLGRNKRAELGVIDFTLAKRALLRDAQVGVLG
                     LTDICDAHPELLRAARHVGEPTKHDCPVCGKVKLVLLAYVYGEGLRDQNGRVWSIDTG
                     LRMTAANPGASCYVVEVCTNCQWNHLREAFTARRSATG"
     gene            7802..8179
                     /locus_tag="VIX62_13850"
     CDS             7802..8179
                     /locus_tag="VIX62_13850"
                     /inference="COORDINATES: protein motif:HMM:NF018008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5861360.1"
                     /translation="MSVTQGATIRVERSGDRGAWRQLVDPPGAIPSGGREADVFSTAG
                     GALTTGFWEREPDTWSFERPYDEVALILEGDADIVTDDGRTLTIGPGDVLVTPKGSAG
                     TWVIRERVVKFWVIYDGGQIHGA"
BASE COUNT         1184 a         2917 c         3030 g         1459 t
ORIGIN      
        1 tctgctcgcc ttcttccggc ggcgagatcg cgccgccggc gtcgctctcg ggctcggcgc
       61 cgccacgaag ctctatcccg ccgtgctcgc tgtgccgctc atcgcgcaac ggctccggga
      121 acggcgtccc gacggcgcga tcgtgcttgc gtggacgacc gcgggagcgt ggcttctggt
      181 gaacctcccg ttcgcggcag ccgcgccgac ggcatggctg acgttcttcc ggttcaacgg
      241 cgagcgcggc gccgacttcg acagcctctg gtacatcgcc tgtcggcatc tggatctctg
      301 cccctcccac cgggcgatca acgtcgcgtc gttcgcgatc ttcctcatct cgatcgcggt
      361 ggtgtgggcg atcaaggtcc ggcggcaccc cgacttcccc aggtggacgc tgggcctgcc
      421 gctgctcgtg gcgttcctgc tgtcgaacaa ggtctactcg ccccaatatg gactctggct
      481 gctgccctgg ttcgcgctgg cgctcccgcg attgtggccg ttcctcgcgt tctcactcac
      541 cgacgtcgcg gtgttcgtga cgcgcttctc gtggttcgga cgcctccagg gcgcgtcggg
      601 ctcgtcccag gcgctgttcg aggtcatggt cctcttgagg gccgccgtgc tgctctggtg
      661 cgtcgtgtcg tggatccgcc gcgagcacga gccgatcgag atcgagcgac gggagcgagc
      721 ggcctctggg gtcctcgaga tcgagagggt tcggtcatga cggacccgtc gccggatgcg
      781 ttcgcgccgg ccacccggag gacgaccggc ggcgacgggg aagccgtgcg gctgcgtgac
      841 gggctgcgcg actcgctgct cgtgttcctc ggagtgcgga tcggcttctc ggtgctgggt
      901 ctcgtggccg tcgggctgat cgctccgcga caaggcccgc cggccgtcgc ggggtggccc
      961 atcgccccgg tgacgtacgg atgggaggcg ctcttcaccg cggcggaacg acaggacgcc
     1021 gcgtggttcc tcacgatcgc gacgcgcggg tacgacgtcg ccgacggcag cgccgcgttc
     1081 ttccccgtgt atccgctgtc gatccggctc gtcgcagggc taccgggcat cggtccgctc
     1141 ggcgccgctc tgatcgtctc gaacgtctgc ttcgccggag cgctcgcgat gctgcacgga
     1201 ctcacccggc tcgaaggtat gccacgagag acagcgcggc tcaccgtgct gtttctcgcg
     1261 atcttcccga cggggttctt cttcctggcg ccctacacgg aaggaccgtt cctgttggcg
     1321 tcgatctcgg cgttctggtt cgctcgccgc gatcgatggt ggttggcggc gctcgcgggc
     1381 gcgctcgccg caggcacccg cagcgtcggg gtgctcatcg cggtcgcgct ctgcgtcgag
     1441 gccgtgctgc agcatcggcg tctcggtcgg acgctcgccc cgcggatcgc ggccgctctc
     1501 gcggtcggcg tgggccccct gctctacttg gcctggtggc gcgtgcagca cggcgacgcg
     1561 accgtgccgt ggacggccca gcgcaactgg tcccgcgaat cgcgatggcc ctggacgacc
     1621 gtggccgagg gcgtcgggga cgcgtggcgc ctcggcagct actggctcgt cgatgcgctc
     1681 gtcgtcggcg tggtgctgct ggccgtgatc gtcggactgc ggtggctgcg tcccacctac
     1741 tcggtctatg cgatcgcgag cctggcgctg ccgctcgtct acgcctggcc gacgcgcccg
     1801 ctgctgtcga tgccacggtt cgtcgctgtg gtcttcccgg cgttctgggt gctggcacgc
     1861 gccgcggaga ggaggcgcat cccgcccccc gccatcgtgg cggtgttcgc tggcggatac
     1921 gcgatcctgg gcgcgctgtt cgtgacgtgg tgggacgtgt tctgaagcgc cggccgggcg
     1981 ccggtgaggc gacgatgacg cggttcccat cgatgaaccg cccgaacgac taggatggtc
     2041 cgaactcggg gggcctcagg aggcaaggtg acgatcaagg ttgcgatcgc ggacgaccac
     2101 tcgctggtgc gccagggcct gcgccgatac ctcgacatgg ccgacggcat cgaggtggtc
     2161 ggcgaggccg ctgacgggaa ggaactgctc gacctgatcg gcaccgagac ccccgacatc
     2221 gcgttgatcg acatccggat gcccgagatg gacggcctcg aggcagctcg tgagatccgc
     2281 gaccgttggc ccgagatcgg cgtgatcatg ctgacggcgt acgacgaccg ccagttcgtc
     2341 gtggaggcag tccgctcggg cgcccgtggc tacgtgttga aggcacgcga cgccgagcac
     2401 ctgatccaga ccgtgcgcct ggtcgcgggc ggcaacatgg tgatcgaccc ccagctggtg
     2461 gtcgcgctcg ccgaagagct ctccaccgcc aaggaacggg accgcaaggc cgagaccctc
     2521 accgcccgcg agatcgaggt gctgcagctc ctcgcgttcg ggcacacgaa ccgcgacatc
     2581 gccgagcgcc tctacatctc acccgacacg gtcaagaccc acctggagca catcttcgag
     2641 aagctcggcg cctccgaccg cacggcagcg gtcgccgaag cgctgcgtcg gcgcctgatc
     2701 gagtagggca ccgggccgcg ctgcgagccg tccgtcccct cgacgggcta gtccgtccgg
     2761 tctgcaacga cccccggtcg ggtgatggag tcgggtgtct ccaccaggca tcatgggcgt
     2821 cacatgagcg acgtcgccgt ccgcccgcgt gcacgcaccg ccacgggcct gttcctcggc
     2881 gccgccgcgg tggccgccgt cgtgacctac ttggcgttcc ccagcggctc cagggagcag
     2941 ggtctggctg ctcaggtcat ggtggcggga tcatgcctgc tgtcgggcgg cctcatcctg
     3001 cgagcggtcg ggaagggcga ggcgtggcgt ggcacggtgc atctcgccta cgccctgttc
     3061 atgcttgggg ccgtcaacgt cgtgttcctc gccgtcgacc tgaacgatgg cggtacctac
     3121 gagccgcggg tcaccgatct ggcgttcgcg ctgttcctcg tgccgctcgc acagctcgcg
     3181 cgtgcggagt tccacgccca tttcgacgcg cgagacggca tcgagatcgg cacggacatc
     3241 ttcctcatca cggcctcggt cacggcgctg atgtacgtgg tcgtacgccc gttcgacgcg
     3301 tcgctccagg catcgatcac cgccgcggtc ttcagtgcgg tcgccgcgtt ccaggtcgcg
     3361 gcgttcggcg ccctcgcctt gtgggcgccc tcgcgacggc acctcgtgct cttcggcatc
     3421 ttcgccgtga cggctgccgc cgccgcgacg ttcgggtggc attggacccg cggctccttc
     3481 gacagcacga tcgcctcgat cgtgctgccg ttcatgctgt gcccgctggc gttcgcggca
     3541 tggaccgtct tcaccggcat ccccgattcg gcgaccggat cgacggcatc gcctcgacgc
     3601 tgggctcgac cggtgctcac cagcctctcg gtggtcacag cctgcgcggc gctcgccgtg
     3661 gtggcgatct tcgacgagga gaggggcatc gacggagccc agtcgaccgc gatcatcctg
     3721 ctgctcggtc tgggcatcgc ggctcgcatc ctctcgaacc agacggcgag cacgatcgcg
     3781 caccgacaga ccgccgaggc gctcggagag cgagaacgcg cgctggccga gaccgaccag
     3841 gcgctcgaac gcgtccgcga agccaacgag acgctccgcc agtccgagga gcaccttcgt
     3901 ttggtcttcg agaccgccgt ggacgggatc gtcgaactcg atgagcgagg cgtcgtgctg
     3961 cgagcgaacg aggcgttcgc cgagatgatc gcgctgccgc gcgagtcgat cgaggatcag
     4021 ccgtggacag ccctggcctc ggtggtcgtc ggggcggacg acgccttcgc cggtctccga
     4081 acgggcgggc agtcgcagat cacgcgcccc ggcgggcagg tgttgcacct cgagtcgcga
     4141 acgtccgccg tgccggcgac tccgccacgc acgctgttgc tcgtccgcga cgtcaccgcg
     4201 gcgcgggtcg ccgatcagac gatccggtcg ctgttccagt tcctgcagga ccgcgacgag
     4261 gaccggagcc ggctgatgcg ccgaacgaac gccgcgatcg aagccgagcg caaccgcatc
     4321 gcgcgcgacc tgcacgacgg gccggtgcag ggcgtctccg cagcgtcgct ctcgctcgag
     4381 gcagcgttgc tgatgatcca agcgggcgac gtcgatcagg gcgtcgaggt gctcacgaag
     4441 atccgcgagg agctcgccgg cgaggccgac gcgctccgcg gtctgatgtc cgggctccgg
     4501 ccaccggtgc tcgaggagcg ggggctcctg ccagctctgc gcgagacact cgtccggttc
     4561 ggcgccgacc agggggtcga caccgagttc gcaggacgcg tcgacgtgcc gctgcccgag
     4621 gacctcgaga cgctcgccta tcgggtggtg caggaggcgt tgtccaacgc cgcgaagcat
     4681 gcgggggcga cccaggtcat ggtcatggtg caggccaacg actcgcagtt gcgggtcgag
     4741 gtggaagacg acggacaggg gttcgacgcg ggactcgcgc gggacttcct gcgcgaaggg
     4801 cgggtcgggc tggcctcgat gcgagaacgt gtcgagctcg cgagcggatc gttcgtggtc
     4861 cgatcctctc ctgggcgcgg cacctccatc gtcgccaccc tgccgctcga tgagctggca
     4921 ccctcgcgcc gcggcaccac cctgcacgag gcgtcgtagc gctactcgtc gggcggcggg
     4981 gtctcgctgg gcggcggggt ctcgctgggc ggtggggtct cgctgggcgg cggcgtttcg
     5041 ctgggttcga cggtcggctc cggcgtctcg ctgggttcag gcgacggaga cgctgacggc
     5101 gtcggggacg cgatcggcgt gggggcaccc aaggtgcccg tcaaggagaa gtccgtcgag
     5161 aacggcagcg gctcccagcc ccactcctcc gccccgtagg ccatcgcctc gagcatgtag
     5221 tcgtgccaga tggccgcggg gatcgtgccc ccgtacacgg tcggcacgcc ggcgacgttg
     5281 atcaaggggc gctgctcctc ggggtagccg acccagacgc acgtcgagag ctgaacggtg
     5341 tagccgcaga accacgcgtc gacgttgtcc tgcgcgctgc ccgtcttgcc ggcggcgggc
     5401 cacgggtaga tcgatgccgc cgaaccggtg ccgccttgga cgacgccctg cagcgccgag
     5461 gtgaccgccc gtggcacatc ggtgtcaccc ttgaagggat ccttcgggcg cgacgcgacc
     5521 ctgcgatcgg ccggctgccc gttcggccgg tcgaccgcca gcaaaggatt ggcgcggtgg
     5581 tatacgccgt ccgccgcgag ggtcgcatag gcattggtca tctcgagcgg gttgatctgg
     5641 acgctgccca acgtgatcga gcacacggcg gggagctccg actggatgcc cagatcgtgc
     5701 gccatgtcga ccacgtcggc cggcccgccg gcgagcgccg acacgagctg tgcatagacg
     5761 gtgttcacgg agttcaccgt ggccccgtag aggtcgaccg tgccggcgga gctgtcgccc
     5821 gcgttcgtcg gggtccagag accgtcggga cccgagcact gcgggtcggc gatcgtcatc
     5881 gtcgacggac ccgaccagta ctgtccccgc agcgcgaacc cttggtcgag cgcggcagcc
     5941 agggtgaacg gcttgaacgc cgatcccgcc tggcgcccgc acccctcaca cgggagggtc
     6001 gccaggttca gcttggacgc gttccagtcc ctgcccccga ccatcgcgag cacctcgccc
     6061 gtctcgttgt cgatcgacac gagcgcagcg tcggggttgt agtcggtgtc cggcaggttc
     6121 gcctcgaccg cgtcctcggc catgcgctgc agcctcaggt ccagggacgt ggtgatctgc
     6181 aggcctccgc cgtacacacg ggcgctgccg tagcgctgga acagctcgga tcgggtccac
     6241 tcgacgaagt actcggagcc ggcggggctc gagatgcggg catcctcgtt cttctgcgac
     6301 ttcgggatgc cgcagcactt cagctccttc aggcgggcgg cctcgctcgg cgggatgtag
     6361 ccgtacttcg ccatctgatc gagcgtgtag tcacgtcgga actcgttgtc gtacttgctc
     6421 ttgatcgggt cgtacaggct cggcgcgtgc agcaccgcgg cgaggacggc cgcctcgttc
     6481 acggtgagct cggaagcggg cttgtcgaag taggtgaggg ccgcggcttc cgcaccgtag
     6541 gcgccgtggc cgaagtacac cgtgttcagg tacttcgcga ggatctggtc cttggtgagc
     6601 tgctgctcga ccttgatcgc gagcagcgct tccctgatct tctccttgat cgtgcgcggc
     6661 ggcacgatgt actcctgggc gccgttcggg tgctcgatgt actcacccgc gtacacgttc
     6721 ttcacgagtt gctgggtgat cgtcgaggcg cccaccacgg tctcgcgctt cacgaggtcg
     6781 gtccacgcgg cgctcacgat gcctcgcacg tcgatgccgg ggtggtcgta gaacccggcg
     6841 tcctcggtcg cgagtacggc gtgctcgagg ttgtcggaga tctgcttcag cccgacgatc
     6901 cggcggtcga cggtgccgtg caatggggtg agcacctcac cgttgcggtc gtacagatag
     6961 ctcgtgcgga tcggcggcag ggtctcgggc agcgagatgc gggcgtaggc catgtagagc
     7021 gatccaccga tcgcgacgac gaccagcagg ggcacgagga cgaacaccca ccagaaccga
     7081 cggaagaacc ccttcttccg cgtgctgggg gcgtgtttgg gaacgtgctt ggccccttcg
     7141 ggcctcgtgg gggttgtggt cgacacgacc tccgattgta tcgacagggt gggtgccgca
     7201 agggaccaga cgtgtggcgg accgatggag atacggttgc gccgcaggga gcgcggccgc
     7261 cgtcggccgc tacccggtgg cgctgcgccg ggcggtgaag gcctcgcgca ggtggttcca
     7321 ctgacagttc gtgcacacct cgacgacgta gcagctcgcg ccggggttcg ccgccgtcat
     7381 cctcaagccg gtgtcgatcg accacacccg cccgttctgg tcgcggagcc cttcgccgta
     7441 gacgtacgcg agcagcacca gcttcacctt tccgcacacc gggcaatcgt gcttcgtcgg
     7501 ttcgccgacg tgccgcgcgg cccgcagcag ctcggggtgt gcgtcgcaga tgtcggtcaa
     7561 cccgagcact ccgacctgcg catctcgcag cagcgcgcgc ttggcgagcg tgaagtcgat
     7621 cacgccgagc tcggcccgct tgttgcgacc cagcggcgtt cgtcgcggcc ggttcccctg
     7681 ctgcttcggc atggtcggga aagcataccc ccggctcacg tcacggcgga cccgccggaa
     7741 gtctcatccc cggtagcctt gcgtcgtggg cccgagggcc cttcgaggag gtagtcggcg
     7801 aatgagcgtg acgcaggggg cgacgatcag ggtcgagcgg tcgggcgatc gaggcgcgtg
     7861 gcgtcagctg gtcgaccctc cgggcgcgat cccgtcaggc ggtcgcgagg ccgacgtctt
     7921 ctcgaccgcc ggcggcgccc tcacgaccgg gttctgggaa cgagagcccg acacgtggtc
     7981 gttcgagcgt ccctacgacg aagtcgcgct gatcctcgag ggcgacgccg acatcgtgac
     8041 cgacgatggg cgcaccctca cgatcggccc cggggatgtg ctggtcaccc cgaaggggtc
     8101 ggccggcact tgggtgatcc gcgagcgcgt cgtgaagttc tgggtgatct acgacggtgg
     8161 ccagatccac ggtgcgtgag gattaggcca ttggtccata gcgaaacacc tgcgcgccgg
     8221 agtacgttcc tgtgaggtcc gcacttcccc cggtccccag gagatggaat gatggacacc
     8281 cccacgccga tgccacgata tggtcggctt acccgatgcc atccccgcgc cgacgttcaa
     8341 gacggcgaaa cgcgggttcg agcgggaaca ggtgctccag catgtcgatc ggctgaacga
     8401 tcgtctgcgc acctcgagca ccaggtgcga cagttacggt tcgagacgga acaggcgaag
     8461 cgggagcgtg acgcggccct ccgagagcga gcaacgctcc tgcaagagcg agacgggggt
     8521 gccgcggcga tgtcgcagcc cgcgcaggag gtcgacacct acgagcacgt gtccatcgcg
     8581 tgtcggggtt
//
LOCUS       DATDBZ010000597         8525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1221525, whole genome shotgun sequence.
ACCESSION   DATDBZ010000597 DATDBZ010000000
VERSION     DATDBZ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221525"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VIX62_13855"
     CDS             complement(<1..878)
                     /locus_tag="VIX62_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061810 - NAD glycohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEY5861361.1"
                     /translation="MKPRIFLGSSGQQEKLLQALTRGLQDIADVDPWTTVFNPGVSTL
                     DRLVELTHEVDFAAFVFARDDWTTKGAVSDATPGEASPRDNVVFEAGLFGGALGIRRT
                     FILHADGAKLPSDLLGLTAIRYDPDTTPAIVRQINQKLRNAIETEGRLTRLEGDWWQL
                     SLTARTELEPSAISLLTISRDRAGALEVAGRSWQADGTLSARYWSEASKERTDPAGIF
                     YFWKGERPRDPDAPELEGTGEIKLESVDRAAGYFTTRSVGGDPFHAKTSGVYLRAEPG
                     DKAALDGDDASGRAAL"
     gene            complement(932..1008)
                     /locus_tag="VIX62_13860"
     tRNA            complement(932..1008)
                     /locus_tag="VIX62_13860"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(972..974),aa:Ala,seq:cgc)
     gene            complement(1058..1489)
                     /locus_tag="VIX62_13865"
     CDS             complement(1058..1489)
                     /locus_tag="VIX62_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861362.1"
                     /translation="MTPRKGMRFMGQAERKRARVRERRRQVFTFLLEAIGFTFLIGLV
                     PPLRVVWGVTAAFTLLLAVYVWLLLTVKHRAAMIRTTPDPRTRGQQARQPATRAAAPA
                     HRYVAEGRSTWARPSFNGLGTVGEGDRVHVVVRTAADLAGA"
     gene            complement(2029..2502)
                     /gene="moaC"
                     /locus_tag="VIX62_13870"
     CDS             complement(2029..2502)
                     /gene="moaC"
                     /locus_tag="VIX62_13870"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY5861363.1"
                     /translation="MAGPSQAGADGVTRMVEVGEKAETAREAIAECFVSMEPEAVRHL
                     LAGTKKGDPIATAKIAGVIGVKETPRLLPHCHPIRVTGADISIEADADGGSLRVTATV
                     RAMDRTGVEMEALTAAAVAALSLYDTAKAFDRAARIDGLRLLAKSGGKSGDYRAD"
     gene            complement(2508..3773)
                     /gene="glp"
                     /locus_tag="VIX62_13875"
     CDS             complement(2508..3773)
                     /gene="glp"
                     /locus_tag="VIX62_13875"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY5861364.1"
                     /translation="MADSLPRVHDHDSAEGLVSVEDARERVLSSISPLSPLALPLTDA
                     YGCVAAEDMVATISLPEFASSGMDGFAVRAADVADASPEHPAELKIVGRAMIGHRPDA
                     TVGGGEAVQIATGAPIPAGADCVVPVENSRVHNETVQLFEAASEGRHVRPAGEDVKEG
                     SVLVEGGKRLGPPELGLLANAGHGTPLVHPRPRVVVLSTGDELIAPTEHPDFGQVRDS
                     NAYTLFGALREAGAMPVLAGIVRDDVDSLKDTVLDYLVQADAFVSSGGVSVGERDVVK
                     AAFFQRGDVSFYKVAMQPGMPQGFGHIEGKPYFGLPGNPVSVFVSFELFVRPALLKMM
                     GRSQVSRPEITARLTQDVTGPKGKMQYARVVVARGSDGWKATPTGARGSNLISTVSRA
                     NGLAMIPPGVELAPEGSQVRVMLFRAAED"
     gene            complement(3807..4685)
                     /gene="galU"
                     /locus_tag="VIX62_13880"
     CDS             complement(3807..4685)
                     /gene="galU"
                     /locus_tag="VIX62_13880"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY5861365.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKAVPKEMLPLIDTPSIQLVVQEA
                     VRSGLTDILIITARGKESIEDHFDRSPELDSLLEARGKFDELKQIRELSEMAQIHYIR
                     QGEAAGLGHAVAVAEPHVGDEPFAVLLGDDLIHPSVPLLPEMLRVVEKYGRSVIAAQE
                     VPKDDISMYGCITPEPVEDNLARVRSIVEKPSPEDAPSNLAAIGRYVFTPEIFNAIRE
                     TKPGKGDEIQLTDAINLLAQDQAVYAYIFDHGRFDVGNKLDYLKATVEFAIERDDLGP
                     EFSSWLADFVQRKKLV"
     gene            5061..5453
                     /locus_tag="VIX62_13885"
     CDS             5061..5453
                     /locus_tag="VIX62_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY5861366.1"
                     /translation="MPTYEYVCKSCGHIFEIVQSMKDDPLTECPECGGELRKVFAPPA
                     IAFKGSGFYATDHGKKSKQPTSGKKESGESSSEGKGSSEQGGSGGESKTDKPAEKPGE
                     KPGEKRTESKPAASGSGSANGSGSGGSS"
     gene            5450..6244
                     /locus_tag="VIX62_13890"
     CDS             5450..6244
                     /locus_tag="VIX62_13890"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY5861367.1"
                     /translation="MTTAEIGVFGGSGFYSFLDDTETIEIETPYGAPSAPITLGDVGG
                     RTVAFIPRHGLKHEFPPHRVPYKANAWAMKELGVTRVLGPNACGSLQAGVKPGDFVIC
                     DQLVDRTRDRPNTFYDGPVTTHISFADPYCPTMRAAAIEQGRALGIPLHDRGTVVVIE
                     GPRFSTRAESTWFSGAGWEVINMTQYPEAILARELELCYMNISLITDYDVGVLGVAPV
                     THEEVVRVFTENNEKLRDLLFAVIPALPDARDCPCATALTGARFEA"
     gene            6271..6468
                     /locus_tag="VIX62_13895"
     CDS             6271..6468
                     /locus_tag="VIX62_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861368.1"
                     /translation="MANEPELVRIELAFRSDQGEDAGALGDRIREATGLIVGRDKLEE
                     FRVRTLPLVEPKRKGGLRPVD"
     gene            6524..7246
                     /gene="cpaB"
                     /locus_tag="VIX62_13900"
     CDS             6524..7246
                     /gene="cpaB"
                     /locus_tag="VIX62_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY5861369.1"
                     /translation="MDLLRRRPPTSALVYFGLAAVCAVAAITLMDAYRQHVEATRPDV
                     GPAVSVVVAAADLVRGTVLAEPALTLRPIPSSLVPPGALILVDDALGRTLVADIADGE
                     VVTRTRVGSSEAGPVAALVPPGLRAFVLRSGVPAGTLRSGDLVDVLATYGAGGGRPYT
                     DTVATEVQVLKVLATAESISASGADTGAQIVVLADAATVETLARASALGVVTVSIAGP
                     DEVGSPGAAMGEASPAPGYLDG"
     gene            complement(7243..>8525)
                     /locus_tag="VIX62_13905"
     CDS             complement(7243..>8525)
                     /locus_tag="VIX62_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015654925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEY5861370.1"
                     /translation="FSLEIGREDYDSPFHVDQVMPGGGTRSLPDSLFEVSSYEGLPRF
                     TRMTVTDRAGDVRADRLLPLCPNGYDRQRIADGGPVTPTFPDDCWSNPFSLGLVWGID
                     RDWATNIVGYGMPLKLPAGRYHIDFSVAPLYADLFDVTPQDGYTTVTLVVRDRRNAAS
                     HPRSAPRAVGDMGSPNPKVPIDEDPDPSIVPDLIPLPSFGIGVRHRNSGDWLSFGANV
                     WVDGPSPLVVEGFRREDEPLMDAYQYFYDGDQQVARARVGSFEYDARHGHTHWHFKQF
                     ATYELLEADKTTAVISEKEAFCLAPTDAIDMLTENAEWRPWLQGLSTACGGPEALWVR
                     EVLPVGWGDTYYQGLPGQSFDVTDLPNGTYYVKVQANPGGHLYESNMANNVRLRKVIL
                     GGRPGHRTVRVPPWHGIDTEGAAARQLLANAHPA"
BASE COUNT         1375 a         2950 c         2875 g         1325 t
ORIGIN      
        1 atgagagccg cccgcccgga ggcgtcgtcg ccgtccagcg cggccttgtc acccggctcg
       61 gcacggaggt aaacgccgga agtcttcgcg tggaacgggt cgccgccgac cgaacgcgtc
      121 gtgaagtagc cggcggcgcg atcgacgctc tcgagcttga tctcaccggt gccctcgagc
      181 tcgggagcgt cgggatcgcg gggtcgctcg cccttccaga agtagaagat gccggccgga
      241 tccgttcgtt ccttcgaggc ctcgctccag tagcgcgcgg acagcgtgcc gtcggcctgc
      301 cacgaccgtc ccgccacctc gagggcaccg gcgcgatcac ggctgatcgt caggagcgag
      361 atcgccgacg gctccagttc ggtcctcgcg gtgagggaca gttgccacca atctccctcc
      421 agccgggtca accgaccttc cgtctcgatc gcgttccgca gcttctggtt gatctgacgc
      481 acgatcgccg gcgtcgtgtc ggggtcgtac cggatcgccg tgagaccgag gagatccgag
      541 gggagcttgg cgccgtccgc gtggaggatg aacgtgcggc ggatgccgag cgcacctccg
      601 aacaggccgg cttcgaagac cacgttgtcg cgcggggagg cctctccagg tgtggcgtca
      661 gacaccgcac ccttcgtcgt ccaatcgtcc cgcgcgaaca cgaaggcggc gaaatcgacc
      721 tcatgggtga gctcgacgag ccgatcgagg gtcgagacac ccgggttgaa gaccgtcgtc
      781 cacgggtcga cgtcggcgat gtcctggagc ccccgggtga gcgcctggag cagcttctcc
      841 tgctgcccgg acgacccgag gaagatgcgc ggcttcatca gtcgagaagg ctaccgctgc
      901 aggcgccggg ggtcgatggc gcggcccaac ctggtggagg tgaacggatt cgaaccgtcg
      961 acctctgccg tgcgaaggca gcgctctacc gggctgagct acacccccag ggatgcggcc
     1021 atgctaacaa aggcccccga cgggccgaag gtgaggatca ggcgccggcg aggtcggcgg
     1081 cggtgcggac gaccacgtgc acacggtcgc cttcgcccac ggtgccgagc ccgttgaagg
     1141 agggcctggc ccaggtgctg cgaccctcgg cgacgtagcg gtgtgcgggg gcggcggcgc
     1201 gcgtggcggg ctggcgcgct tgctgaccgc gggtgcgcgg gtccggcgtg gtgcggatca
     1261 tcgcggcgcg gtgcttcacg gtgagcagca gccagacgta gacggcgagc aggagcgtga
     1321 aggcggcagt gacaccccac acaacgcgga gcggcggcac gagtccgatg aggaacgtga
     1381 agccgatcgc ttcgaggagg aaggtgaaga cctggcggcg acgctcacgg acgcgtgccc
     1441 gcttgcgttc ggcctggccc atgaaccgca tgcccttacg tggggtcacg atccaacgac
     1501 cttcggttcc atgaatctct gcttgggcca aaagctccat cctgcgctcg tgttcgccca
     1561 cactgtggtg ggccttcctc ccgcggcgac cggtggggac caggaacgcc acccacatga
     1621 tgcctaacgc ggcgaggagc aaccagtcca acacgagcct cccgtggggt cggagcgccg
     1681 ggacggcaca acatctgggc tggaccggga agctagcacc acatctaggg gctcacaatc
     1741 acatccatgt gattcggaga actttttcac aagccgcctt ctggacgcct tgcgttcatg
     1801 ttggtgctgt gggccggttg acggacgtcg aaaccgtttg cgctcgtggc cggggtcgtg
     1861 ggccggaggg atccggtggg cggcccggac gcggccgacc tgccgaggag tgctcccagc
     1921 ggatcgtcaa caggtgtcca tgttgcgacc tagtgcggaa cccgtacgcc gttgacgatc
     1981 cccggcggga aggtccgagg gataccgaaa agccaggacg cgccggcgct agtccgcccg
     2041 gtaatccccg ctcttgccgc ccgacttcgc cagcaggcgc agcccatcga tccgggccgc
     2101 ccggtcgaac gccttcgcgg tgtcgtagag cgacagggcc gccacggcag ccgccgtcag
     2161 cgcttccatc tccacgcccg tacggtccat cgctcgcacg gtcgccgtca cgcgcagcga
     2221 acctccgtcg gcatccgcct cgatcgagat gtcggcgccg gtcacccgga tcgggtggca
     2281 gtgcggcaag agccgcggcg tctccttcac cccgatcacg ccggcgatct tcgcggtcgc
     2341 gatcgggtca cccttcttcg ttcccgccag caggtgacgc accgcctccg gttccatcga
     2401 cacgaagcac tccgcgatcg cctcgcgcgc ggtctcggcc ttctcaccca cctcgaccat
     2461 ccgcgtgacg ccgtccgcgc ccgcctgcga aggcccggcc accgctctca gtcctccgca
     2521 gcgcggaaga gcatgacgcg cacctgcgag ccctccggcg ccagctcgac ccccggcggg
     2581 atcatcgcca gcccgttcgc ccgcgacacc gtcgagatca ggttcgagcc gcgagcaccg
     2641 gtgggcgtgg ccttccaccc gtcggacccg cgcgccacca cgacccgcgc gtactgcatc
     2701 ttccccttgg gccccgtcac gtcctgggtg aggcgggcgg tgatctcggg tctcgacacc
     2761 tgcgagcgac ccatcatctt cagcaacgcc ggtcgcacga acagctcgaa gctcacgaac
     2821 acgctcaccg ggttccccgg cagtccgaag tagggcttgc cctcgatgtg accgaacccc
     2881 tggggcatgc ccggctgcat cgccaccttg tagaagctca cgtccccccg ctggaagaac
     2941 gcggccttca cgacgtcgcg ctcccccacc gagaccccgc ccgaggacac gaacgcgtcg
     3001 gcctggacca ggtagtcgag cacggtgtcc ttcagcgagt cgacatcgtc gcgcacgatg
     3061 cccgccaaga ccggcatggc gcccgcctcg cgcagcgctc cgaacagcgt gtacgcgttc
     3121 gagtcacgga cctgaccgaa gtcgggatgt tccgtcggcg cgatcagctc atctccggtc
     3181 gagagcacca ccacccgtgg cctcgggtgg acgagcggcg tcccgtgacc cgcgttcgcg
     3241 agcaacccca actcgggcgg cccgagccgc ttgccgcctt cgacgagcac gctgccctcc
     3301 ttcacgtcct cgcccgcggg acggacgtga cggccttcgc tcgcggcctc gaagagctgc
     3361 acggtctcgt tgtgcacccg gctgttctcg acgggaacga cgcaatcggc tccggcgggg
     3421 atcggcgcgc cggtcgcgat ctgtaccgcc tcgccaccac cgacggtcgc gtccggtcga
     3481 tgtccgatca tggcgcggcc gacgatcttc agctcggcgg gatgttcggg cgaggcatcg
     3541 gcgacgtctg ccgctcgcac cgcgaacccg tccatgccgc tggaggcgaa ctccggcaag
     3601 gagatcgtgg ccaccatgtc ctcggccgcc acgcatccgt aggcgtcggt cagcggtagc
     3661 gccaacgggg acagcggcga gatcgacgac agcacgcgtt cacgcgcatc ctcgaccgag
     3721 accaagcctt cagcggagtc gtgatcgtgg acacggggaa gcgagtcggc catgggcgca
     3781 gtctaccgcc gcccgtcgaa cgcccctcag acgagcttct tgcgctggac gaagtccgcg
     3841 agccagctgc tgaactccgg gccgaggtcg tcacgttcga tcgcgaactc gacggtggcc
     3901 ttgaggtagt ccagcttgtt gcccacgtcg aagcggccgt ggtcgaagat gtaggcgtac
     3961 accgcctggt cctgggccag caggttgatc gcatcggtga gctggatctc gtcgcccttt
     4021 cccggcttcg tctcacggat cgcgttgaag atctccgggg tgaacacgta ccggccgatc
     4081 gcggccaggt tcgacggcgc atcctcgggc gagggcttct cgacgatcga gcggacgcgc
     4141 gccaggttgt cctccaccgg ctcgggcgtg atgcagccgt acatggagat gtcgtccttg
     4201 ggaacctcct gggccgcgat gacgctgcgg ccgtacttct ccacgacccg cagcatctcg
     4261 ggcaacaggg gcaccgacgg atggatgagg tcatcgccca gcagcaccgc gaagggctcg
     4321 tcgcccacat gcggttcggc gacggcgacc gcgtgtccca ggcccgcggc ttcgccctga
     4381 cggatgtagt ggatctgggc catctccgag agttcacgga tctgcttcaa ctcgtcgaac
     4441 ttgccgcggg cctcgagcaa agaatccagc tcgggcgagc ggtcgaaatg atcctcgatc
     4501 gactccttgc cccgagccgt gatgatcaag atgtcggtga gcccggagcg gaccgcttct
     4561 tggacgacga gctggatcga cggcgtgtcg atcagcggca gcatctcctt gggcacggct
     4621 ttggtggccg gcaggaaccg ggtgccgaga ccggcggcgg ggatgacggc cttccgcacc
     4681 ttcatggcgt caccttcatg accgactcca ttcccagggg acggcgagtg tacgcgtgag
     4741 gtctccgctc cgctagtcgt ccgttccggg tggtccatcc tgagtagccc tcctcgagat
     4801 cggcccggtc gagcctgacg aagtggccgg acccggcggg gggagccgcc aggcccggca
     4861 tcgcctcgtc cgtggggacg ggacgcatcg agggtcgcac gtgcgggcgc gccccccgtt
     4921 caggggatga ccgtagatgg cggcgggtga gctgcgcatc gggcacatgg ccccggtgca
     4981 tcgccatcgg tccagggccg aacgacgcag tggtgcgcgc gcggaagtgg cggtcgggga
     5041 gccgatacac tcatccggcc atgcccacct acgaatacgt ctgcaagagc tgcggacaca
     5101 tcttcgagat cgtgcagtcc atgaaggacg atccgctgac cgagtgtccc gagtgcggcg
     5161 gcgaactccg caaggtgttc gcgccccccg ccatcgcctt caagggctcc ggcttctacg
     5221 ccaccgacca cggcaagaag tccaagcagc ccacgtcggg caagaaggaa tccggcgagt
     5281 cgagctccga aggcaagggg tcgagtgagc agggcggctc cggcggcgag tccaagaccg
     5341 acaagcccgc cgagaagcca ggtgagaagc caggcgagaa gaggaccgag tcgaagcccg
     5401 ccgcgtccgg atcggggagc gccaacggct cgggatcggg agggtcgtca tgaccacggc
     5461 ggagatcggt gtgttcggcg gatcggggtt ctattccttc ctggacgaca ccgagacgat
     5521 cgagatcgag accccgtacg gcgcccccag cgcgccgatc acccttggag acgtgggtgg
     5581 acggacggtg gcgttcatcc cacggcacgg cctgaagcat gagttcccgc cccaccgcgt
     5641 gccctacaag gccaacgcct gggcgatgaa ggaactcggc gtcacccgcg tgctgggccc
     5701 gaacgcgtgc ggctcgttgc aggcgggcgt gaagcccggc gacttcgtga tctgcgacca
     5761 gttggtcgac cggacccgtg accgccccaa cacgttctac gacgggcccg tcaccacgca
     5821 catctccttc gcagacccgt actgccccac gatgcgggcc gcggcgatcg aacagggacg
     5881 tgccctcggc atcccactcc acgatcgcgg caccgtcgtg gtgatcgaag gaccgaggtt
     5941 ctccacgcgc gccgaatcca catggttctc tggcgcgggc tgggaggtca tcaacatgac
     6001 ccagtacccc gaagcgatcc tggctcgcga actggagctc tgctacatga acatctcgct
     6061 catcaccgac tacgacgtcg gcgtgctggg cgtggctccg gtgacgcatg aagaggtcgt
     6121 ccgggtgttc accgagaaca acgagaagct ccgcgacctg ttgttcgccg tgatcccggc
     6181 gctcccggac gcgcgcgact gcccgtgcgc cacggccctc acgggcgcgc gcttcgaggc
     6241 ttgatccgca aagcccgtat cctgcctgac atggcgaacg aaccggagct ggtgcgcatc
     6301 gagctggcgt tccgttcgga ccaaggtgag gacgccgggg cgctcggcga ccgcatccgg
     6361 gaagccaccg ggctgatcgt gggacgcgac aagctcgagg agttccgcgt caggacgctc
     6421 ccgctggtcg agcccaagcg caaaggcggg ctccgcccgg tcgactgaac cccgtgtgac
     6481 cgcggtcacg gcgacacccc cggtgcgccg atagcgtcgc ggcatggacc tgcttcggcg
     6541 tcgcccaccc acgtccgcgc tcgtgtattt cggcttggcg gccgtgtgcg cggtggccgc
     6601 catcaccttg atggatgcct atcgtcagca cgtggaggcc acgcgtcccg acgtcggtcc
     6661 ggccgtgtcc gtcgtggtcg ccgcggccga cctggtgcgc ggcacggtgc tcgccgaacc
     6721 ggcgctcacc ctacgtccga tcccctcgtc cctggtgcca cccggcgccc tcatccttgt
     6781 cgacgacgcg ctgggccgga ccctggtggc cgacatcgcc gacggcgaag tcgtgacccg
     6841 aacccgcgtg ggcagctccg aggccgggcc ggtcgccgcg ctggtgcctc cagggctccg
     6901 ggcgttcgtc ctgcgttcgg gggtgcccgc cggcaccctg cgatcgggtg acttggtcga
     6961 tgtgctcgcc acctatggcg ccggcggtgg gcggccgtac accgacaccg tggccaccga
     7021 ggtgcaggtg ctcaaggtgc tcgccactgc cgaaagcatc agcgcctcgg gagccgacac
     7081 cggcgcgcag atcgtggtgc tcgccgacgc cgcaacggtg gagacgctgg cgagagcatc
     7141 ggccctcggc gtcgtcacgg tctcgatcgc cgggcccgat gaagtcgggt cccccggcgc
     7201 ggcgatgggg gaagcatcgc ccgcgccggg gtacctcgac ggctaggccg ggtgggcgtt
     7261 cgccagcagc tgtcgcgccg ccgcgccctc ggtgtcgatc ccgtgccacg gcggcacccg
     7321 aacggtgcgg tgtccgggcc tgccgcccag gatcaccttg cgcaaccgaa cgttgttggc
     7381 catgttcgac tcgtacaggt ggccgcccgg gttggcctgc accttcacgt agtaggtccc
     7441 gttgggcagg tccgtgacgt cgaacgactg gccgggcagt ccctggtagt aggtgtcgcc
     7501 ccaacccacg ggcagcactt ctcgcaccca cagcgcttcc ggaccgccgc acgcggtact
     7561 caatccctgg agccagggtc gccactcggc gttctcggtc agcatgtcga tggcgtcggt
     7621 gggagcgagg cagaaggctt ccttctcgct gatcacggcg gtggtcttgt cggcttccaa
     7681 cagctcgtag gtggcgaact gcttgaaatg ccagtgggtg tgcccgtgcc gagcgtcgta
     7741 ctcgaacgag cccacgcggg cgcgcgccac ctgctgatca ccgtcgtaga aatactgata
     7801 ggcatccatc agcggttcgt cttcgcggcg gaagccctcg accaccagcg gcgacgggcc
     7861 atccacccac acgttcgctc cgaaggacag ccaatccccc gagttccgat gacggacccc
     7921 gatgccgaac gagggcaacg ggatcaggtc gggcacgatc gaaggatcgg ggtcctcgtc
     7981 gatcggcacc ttcgggttcg gtgagcccat gtcgccgacg gcgcgcggcg ccgaccgtgg
     8041 atgggatgca gcgttacgac gatcgcggac gacgagcgtc acggtcgtgt acccgtcctg
     8101 cggcgtcacg tcgaacaggt ccgcatagag cggagccacc gagaagtcga tgtgatagcg
     8161 ccccgccggc agcttcaacg gcatcccgta tccgacgatg ttggttgccc agtcgcggtc
     8221 gatcccccac accagaccga gcgagaacgg gttcgaccaa caatcgtcgg ggaacgtggg
     8281 cgtcaccggt ccgccgtcgg cgatgcgctg acggtcgtag ccgttcggac acaacggcag
     8341 cagacggtcg gcccgcacgt cacccgcccg atccgtgacc gtcatccgcg tgaatcgagg
     8401 gaggccttca taggacgaga cctcgaacaa cgaatccggg agcgaacggg ttccgccgcc
     8461 cggcatcacc tggtccacgt gaaacgggga gtcgtagtcc tcgcgcccga tctcgaggga
     8521 gaacg
//
LOCUS       DATDBZ010000598         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_192755, whole genome shotgun sequence.
ACCESSION   DATDBZ010000598 DATDBZ010000000
VERSION     DATDBZ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192755"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VIX62_13910"
     CDS             complement(<1..246)
                     /locus_tag="VIX62_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5861371.1"
                     /translation="MKIPTEFGYARAADGAYIAYRTMGAGPIDLVWQFDWLSNVDTLP
                     ESDYLGPLFSSLASFSRVILRDRRATGLSSKNVAVPNL"
     gene            complement(243..1592)
                     /locus_tag="VIX62_13915"
     CDS             complement(243..1592)
                     /locus_tag="VIX62_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5861372.1"
                     /translation="MTQAPETRYARAADGIHVGYQVVGSGPVDIVFVPYDYSNIEAAW
                     DFPPFVSFVRGLATIGRVLVFDRRGSGTSDRSWTGEAATMEAQMDDIRAVMDAASSER
                     AFVFGIESGAALCFMFAATHPERTSGVIVQAPMLRGTPAPDYPGSWSREMYEEYFERI
                     EQAWGTDAFVREEVEQLSPSLLHDEAIARAFGKQLRLSASPGDAIARNRAVMETDVRH
                     ILPSVQAPTLVLHRTGNRFTKVAEGRWTAEHTPGARIVELPGEDHVYPLDDLVPHIAA
                     FVESLRVEQADFDRVLATVLFTDVVDSTVQAAALGDAGWRDVRARHDQIVRSQIARYR
                     GREIKTMGDGFLATFDGPARGVRCATAIAAAVGPLGIEIRAGLHTGEVTIEGDDVAGL
                     GVVIGARVGALAGPAQVLVSQTVKDLVAGSGLIFEDAGDHELKGVPDRWRLYRVAGG"
     gene            complement(1819..>2103)
                     /locus_tag="VIX62_13920"
     CDS             complement(1819..>2103)
                     /locus_tag="VIX62_13920"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY5861373.1"
                     /translation="WTEAKDLLRDQGIAWCVAETDERDPTPEELSWDPVGYLRLRKTE
                     YGDDELEEWVGRIHPALESGADIFCYFKHEDLGASPQMAKRLEDILRSKA"
BASE COUNT          346 a          744 c          672 g          341 t
ORIGIN      
        1 gaggttcggg accgcgacgt tcttgctgga gagcccggtc gcccggcggt cgcgcaggat
       61 cacccgcgag aacgaggcga gcgacgagaa caagggcccg aggtagtcgg actccgggag
      121 cgtgtcgacg ttgctgagcc aatcgaactg ccacaccagg tcgatcggac ccgcgcccat
      181 cgtccggtac gcgatgtagg cgccgtccgc ggcgcgcgcg tagccgaact ccgtggggat
      241 cttcatccgc ccgccacccg gtagaggcgc cagcggtcgg ggacaccttt caactcgtga
      301 tcgcccgcgt cctcgaagat gagaccgctc cccgcaacga gatccttgac cgtctgggaa
      361 acgagcacct gcgcgggacc ggcgagggcg cccacgcggg cgccgatcac cacgccaagg
      421 ccagcaacgt catcgccctc gatcgtgacc tcgcctgtgt gcaaccccgc cctgatctcg
      481 atcccgagag gacccaccgc cgcggcgatc gccgtcgcgc agcgcacccc gcgtgccggc
      541 ccgtcgaacg tcgcgaggaa cccatcgccc atcgtcttga tctcgcgtcc gcggtagcgg
      601 gcgatctgcg agcgcacgat ctgatcatgg cgagcacgca catcccgcca gccggcatca
      661 ccgagcgcgg ctgcctgcac ggtggagtcg accacgtccg tgaacagcac cgtcgcgagg
      721 acgcgatcga agtccgcttg ctcgacgcgg agcgattcga cgaacgccgc gatgtggggg
      781 accagatcgt cgagcgggta gacgtggtcc tctcccggga gttccacgat gcgcgccccg
      841 ggcgtgtgct ccgcggtcca tcgcccctcg gccaccttcg tgaaccggtt gccggttcga
      901 tgcagcacga gcgtcggagc ttgaaccgaa gggaggatgt ggcggacgtc ggtctccatc
      961 accgctcggt tgcgggcgat tgcgtctccc gggctcgccg agagccggag ttgcttcccg
     1021 aacgcgcgcg cgatcgcctc gtcgtgcagg agcgagggcg acagctgctc cacttcttca
     1081 cggacgaagg cgtcggtgcc ccacgcctgc tcgatgcgtt cgaagtactc ctcgtacatc
     1141 tctcggctcc acgacccggg gtagtcgggc gccggggtcc cgcgcagcat cggcgcctgc
     1201 acgatcaccc cggatgtgcg ttccgggtgc gtcgcggcga acatgaagca gagcgccgcg
     1261 ccgctctcga tcccgaacac gaacgcgcgc tcggatgacg ctgcatccat cacggcgcgg
     1321 atgtcgtcca tctgcgcttc catggtggcc gcttcaccgg tccacgagcg gtccgacgtg
     1381 ccgctgccac gccgatcgaa gaccaacacc ctgccgatcg tggccaagcc ccgaacgaac
     1441 gagacgaacg gggggaagtc ccaggcggct tcgatgttgg agtagtcgta cgggacgaac
     1501 acgatgtcca ccggaccgga acccaccact tggtagccca cgtggatccc gtccgccgcg
     1561 cgcgcgtacc gtgtctcggg ggcttgggtc atgacgccac ccggtacaga cgccaccggt
     1621 cgaggacgcc cttcagttct cgctcgcttg cgccttcgaa cgaaagacca ctgcctgcga
     1681 cgaggccctt cacggtctgc gacacgagga cctcgttggg ttcttcacgt cgtcagtctc
     1741 gttcgcgctg gacgccaggt gcaagttgtt gcccagactg ttgcccagac ctcggtcccg
     1801 caagaggtca catctgcttc atgctttgga gcgcaagatg tcttcgagac gcttcgccat
     1861 ctgtggactg gccccgagat cctcatgctt gaagtaacag aagatgtcgg ccccggactc
     1921 gagcgccggg tggatccgac ccacccactc ctcgagctcg tcgtcgccat actcggtctt
     1981 gcgaagccgc aggtacccca ccgggtccca cgagagctcc tcgggtgtgg gatcgcgctc
     2041 gtcggtctcc gcgacgcacc acgcgatgcc ctgatcgcgc agcaggtcct tcgcctcggt
     2101 cca
//
LOCUS       DATDBZ010000599         2933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2024640, whole genome shotgun sequence.
ACCESSION   DATDBZ010000599 DATDBZ010000000
VERSION     DATDBZ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024640"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VIX62_13925"
     CDS             complement(<1..501)
                     /locus_tag="VIX62_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY5861374.1"
                     /translation="MRALVLSGGEGSRLRPITHTSAKQLIPVAGTPILFHALEAIADA
                     GITEVGIVVGQTAAEVRSAVGDGSRWGLQVTYIPQDAPLGLAHAVITAREFVQGQPFL
                     MYLGDNVLLEGLSRFVQEFERNRPDAQIFVARVPEPERFGVAVLDGDRVVRLVEKPRE
                     YVSDLAL"
     gene            complement(501..1973)
                     /locus_tag="VIX62_13930"
     CDS             complement(501..1973)
                     /locus_tag="VIX62_13930"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY5861375.1"
                     /translation="MSDGPSADPRAHGRLARAAVAITATLSLLIGIGSAYGFVAYQQA
                     KGVGTVDDFDTDDVPKASNEPASPGGCADDVCNYLLLGSDSRQGLSQEEQTEFGTDQQ
                     IGGENRADTIMLVHTDPKLEKAIIVSFPRDLYVEIPGHGEDKINAAFEGGVEGGGPTL
                     VANTVQQLTGLKIDHFLYVDLAGFQGVIDELDGVDMCITGENVNTPGYVESETADGTI
                     SEVYYNEPGHIVDPRTGLDVKPGCQRLPSDQALAYVRTRHLKCDSAAPDFYRIQRQQQ
                     FLRSVINRLLQPEQLAQLPLMIKPILSNLRRDEGLNPAELAYLTGQLQGISSGATEFR
                     TVPAYPDPSNLGILRMDQSAERIFAAIRQGKALGTVGEDTVYTPPSEATVPVLVVDHA
                     SGGKVTQVTDVLSQAGFDITPGVTTYPEYSKKLAGNVIAYAPDADAKAQVVAKYFPNL
                     ELQEVEGLPDDVVVFVDATYKPVPVGGGSTAESTCPEPNV"
     gene            2135..>2933
                     /locus_tag="VIX62_13935"
     CDS             2135..>2933
                     /locus_tag="VIX62_13935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5861376.1"
                     /translation="MDDAVTVMLVGLAPAMADELADDDRFDVRSADTVDQIGGDDHVD
                     AVVIAMDGQGPLDLIGAVHARAPDAAVVVVTEAEHEADGAVAMHAGAEDHLVLGSIPE
                     GLLPRAVRYAVSIRRLRRELATTDEVTGLPNLRGFVPIAEHHLRMADRSKAPVVFLFV
                     RLDGEPAPEQARQAAEVLLEAVRDADLPARITEDTFTVLLTGEAVGAETLVLSRLVEA
                     IAVHNAKQDPPQPLSLSVGSAIYDPEQPTTLSQILETAGRRLHDQAEG"
BASE COUNT          455 a          985 c         1019 g          474 t
ORIGIN      
        1 gagcgcgagg tcgctcacgt actcgcgagg cttctccacg aggcgcacca cccggtcgcc
       61 gtcgagcacc gccacgccga agcgttcggg ctcggggacg cgcgccacga agatctgcgc
      121 gtcgggacgg ttccgctcga actcctggac gaaccggctg agcccttcga gcagcacgtt
      181 gtcgcccagg tacatcagga agggctggcc ctgcacgaac tcgcgcgcgg tgatcacggc
      241 gtgggccagg cccaacggtg catcctgcgg gatgtacgtg acctgcagac cccagcggga
      301 cccatcgccc accgcgctgc gaacctcggc cgcggtctga cccacgacga tgcctacctc
      361 ggtgatgccg gcgtcggcga tcgcctccag ggcgtggaac aggatcgggg tgccggcgac
      421 cgggatcagt tgcttggcgc tggtgtgcgt gatggggcgg agccgggagc cctcgccgcc
      481 cgacaacacc agggctcgca tcagacgttc ggctccggac aggtggactc ggccgtggag
      541 ccaccaccga cgggcacggg cttgtaggtg gcgtccacga acacgaccac atcgtccggc
      601 aggccctcga cctcctgcaa ctccaggttg gggaagtact tcgccacgac ctgagccttc
      661 gcatcggcgt cgggtgcgta ggcgatcacg ttgcccgcaa gcttcttcga gtactcgggg
      721 taggtcgtca cgccgggagt gatatcgaac ccggcttgcg agagcacgtc cgtgacctgg
      781 gtcaccttgc cgccggacgc gtgatcgacc accaggacag gcacggtggc ttctgacggc
      841 ggcgtgtaca ccgtgtcctc cccgaccgtt cccaaagcct tgccctgccg gatcgcggcg
      901 aagatccgct cggccgactg atccatccgc aggatcccga ggttcgaggg gtccgggtag
      961 gcggggaccg tgcggaattc ggtcgctccg gaggagatcc cttgcagctg gcccgtgaga
     1021 taggcgagtt ccgccgggtt gagcccttcg tcccgccgca ggttcgacag gatcggcttg
     1081 atcatcaagg gcagctgcgc caactgctcg ggctggagca ggcgattgat caccgaacgc
     1141 aggaactgct gctggcgctg gatccggtag aagtccggcg ccgccgagtc gcacttgagg
     1201 tggcgggttc ggacgtaggc cagcgcctga tcagacggca accgctgaca ccccggcttc
     1261 acatcgaggc cggtccgcgg gtccacgatg tggccgggtt cgttgtagta gacctcgctg
     1321 atcgtgccat cggccgtctc ggactcaacg tagcccggag tgttcacgtt ctcgcccgtg
     1381 atgcacatgt ccacgccgtc gagttcatcg atcacgcctt ggaacccggc gaggtcgacg
     1441 tacaggaagt gatcgatctt caaccccgtg agctgctgca cggtgttggc caccaaggtc
     1501 ggcccaccgc cttcgacgcc tccttcgaac gcggcgttga tcttgtcctc gccgtggccg
     1561 gggatctcga cgtagaggtc gcgcgggaag gacacgatga tggccttctc gagctttgga
     1621 tcggtgtgga ccagcatgat cgtgtcggcc cggttctcgc cgccgatctg ctgatcggtc
     1681 ccgaactccg tctgctcctc ctggctgagg ccctgccggg aatcgctgcc gagcaagagg
     1741 tagttgcaaa catcgtcggc acatccgccc ggtgacgcgg gctcgttgga tgccttcgga
     1801 acgtcgtcgg tatcgaagtc atccaccgtg ccgacgccct tggcctgctg gtaggcgacg
     1861 aagccgtagg cgctgccgat cccgatcagc aacgagagcg tggcggtgat cgcgacggcc
     1921 gcgcgggcga gcctgccgtg cgcgcgaggg tcggcggatg gaccgtcgga catgcagcca
     1981 agcgtacccg gggcctccgc cgccccgctc ctgcgcggcc gtggcgatcc tgtggcggaa
     2041 ccgtggcggc gcacggggtc gcgatgctgc cgggcgcgga ggagcctgcc cgatgcgatg
     2101 ggtgggataa tcccgccacc gaccaggagg aacgatggat gacgcggtga cggtgatgct
     2161 cgtggggctg gcgcctgcga tggccgacga gctcgctgac gatgatcgct tcgatgtgcg
     2221 atcagccgac acggtcgacc agatcggggg cgatgaccac gtcgatgcgg tggtcatcgc
     2281 gatggacggg caagggccgc tcgatctcat cggcgcggtc cacgccaggg ctcctgatgc
     2341 cgccgtcgtg gtcgtgaccg aggcggaaca cgaggccgac ggtgccgttg cgatgcatgc
     2401 cggcgccgag gaccacctcg tgctcggctc gatccccgag ggcctgctcc cgcgagccgt
     2461 gcggtacgcg gtgtcgatcc ggcgcctccg tcgggagctg gcgaccaccg acgaggtcac
     2521 cggcctgccc aacctgcggg gcttcgtgcc gatcgccgaa caccacctgc gcatggccga
     2581 tcgcagcaag gcccccgtgg tgttcctgtt cgtgcggctc gatggcgagc ccgcgcccga
     2641 acaggctcgc caggccgccg aggtgctgct cgaggcggtg cgcgacgcgg acctgccggc
     2701 gcgcatcacc gaggacacgt tcaccgtgct gctcaccggt gaggccgtcg gggccgagac
     2761 actcgtgctg tcgcggctcg tcgaagcgat cgccgtgcac aacgcgaagc aggacccgcc
     2821 gcagcccctg tcgctctcgg tcggcagcgc catctacgat cccgagcaac ccacgacgct
     2881 gtcgcagatc ctcgagacgg ccgggcggcg cctgcacgat caagcggagg gct
//
LOCUS       DATDBZ010000600         6899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_607831, whole genome shotgun sequence.
ACCESSION   DATDBZ010000600 DATDBZ010000000
VERSION     DATDBZ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607831"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..570)
                     /locus_tag="VIX62_13940"
     CDS             complement(25..570)
                     /locus_tag="VIX62_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein FlaR"
                     /protein_id="HEY5861377.1"
                     /translation="MEDPAGAIGHRVLVIGMAGSGKSTFSRALSAKTGLPVIHLDLYE
                     WKPGWVRPSEEEWRAAQRRVLAGNAWIADGNDLDTLELRLERADTVVLLETPWWTCAA
                     RAFRRGLSKPVGEMPEGCEDSAWRRLRDEWGLVGIILRHRGSEVERGREIVARHGRHA
                     VSYALGSKRAARAFLDGLGGD"
     gene            complement(626..1213)
                     /locus_tag="VIX62_13945"
     CDS             complement(626..1213)
                     /locus_tag="VIX62_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5861378.1"
                     /translation="MNLRSVIKGVTLAATAAGVGYAALRTTDGERLDRALFEAGNTDA
                     GPGADRFFLGVTELGSLYAAAAAAGVLAVSGRPREGVRALSAAGATWLLGQGLKKAVN
                     RPRPYEADAEATRFLIAPPHGTSWPSSHPAVVTAFMSVAARELGIGGLGSAGLTSVSA
                     SVAASRVYVGVHYPSDVASGFLLGRAVAALWPRSR"
     gene            1232..1543
                     /locus_tag="VIX62_13950"
     CDS             1232..1543
                     /locus_tag="VIX62_13950"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY5861379.1"
                     /translation="MAATLVTGGAKPMLEDNTGRDDFDPGQPKGSKVKARTILLIVVV
                     VAFGLFLLQNPQEVRISFTFIEVRAPLWLAIAVAGLLGLVAGWLMGRFELGKGRMNKQ
                     R"
     gene            complement(1540..1989)
                     /locus_tag="VIX62_13955"
     CDS             complement(1540..1989)
                     /locus_tag="VIX62_13955"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5861380.1"
                     /translation="MTTVEFSVEVEAPPEQVWTVASDPRNLPHWDRHVVSVALPEGGM
                     KLGAHYSVVMGFMGVNSTVRAEVLEWEPPWRSKVHLSGLLDAIVTTSVGSLPYERSML
                     RHEVDYRFRGPLGGFAAASLNAVGGAQMGLRHGVLAQKAEIESMAQR"
     gene            complement(1986..2933)
                     /locus_tag="VIX62_13960"
     CDS             complement(1986..2933)
                     /locus_tag="VIX62_13960"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5861381.1"
                     /translation="MSFLSTAVQQVLDEGPFCAVATATPRGPHCTPLVFAASGGRIWL
                     TTSRGSVKTRAWKVDPSVSGLVRSGGLSVTFTGHVKTYDALDRGTWGAAVAGATSIAR
                     ATAKFSKKNARFFAGYALDARQVPLAWTPPGRVFVGIDLERTALLDEDGVQEGRGRWG
                     GEAVSHPTFRSHKQGPDPLAELPADIAASLGRSGEGVLTVAGARGPVSLPARWLAEPS
                     ALYVALPAETLALAGAGPNAAMALTIDQVSEWRARDMVGAMVQGEGSIYLMDSLGSGA
                     KTAKALAAGLHPGSDALVRLAPSRLVWWKGWSSGSAPVG"
     gene            complement(2938..3591)
                     /locus_tag="VIX62_13965"
     CDS             complement(2938..3591)
                     /locus_tag="VIX62_13965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY5861382.1"
                     /translation="MQWAVMVAGLLAEIVGWWFVSARGRDVWRLMPIVLGTMGVAAVL
                     ARPPVAAAEVTVTTALAAGLASGLALFLSTRVFVWAAAHWEPFRRDVVEKYSEAAEVS
                     LRRSLLLSLVIMVPSEELFWRGLVQGRLAVAMAAGAAAVAAWVGYVVANISSRSLPIV
                     AGAVVGGALWTGLAWWSGGVLASLASHILWTGLMLALPPGAGRPRFRFRSDAQTGER"
     gene            complement(3595..4371)
                     /locus_tag="VIX62_13970"
     CDS             complement(3595..4371)
                     /locus_tag="VIX62_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861383.1"
                     /translation="MTGAAGYMALVLAEAVAGSLAFLWCVPLWNEVKRGFFTLTSSIL
                     LVLAVAAWWSAKVALVPGENAGLWSVRLALATAIAVGVTFLLMMAKQQTVARVVGLLS
                     VPLSIAVLAAMAGTGRQAYALSLFQLLAGAAFLGTITDGLLLGHWYLTDRGLPRGPIN
                     RYTTAMLIAVGIEAVAVISGGFAATADSGSLNPLLTAGALAPWIALGMVGTTALIAVL
                     TRAALKGERASAVQSATGFYYLAVVTGFTAEVAFKTRFLG"
     gene            4461..5252
                     /locus_tag="VIX62_13975"
     CDS             4461..5252
                     /locus_tag="VIX62_13975"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5861384.1"
                     /translation="MDDWRSYDDVAETYERVHASRFVQVADDLVAQAGIGEGHLLLDI
                     GTGTGIAAEAATKVGARVVGIDESMGMLHVARRQRPAEAVAAAEAIDLPFRDAVFDAA
                     IGSFVLAHFTKYETAIFDAMRVLKVGGTIGFSSWSDGVDAYQTAWSELVESVVPREIL
                     EPAYAEARPWHDKFRKRDQIEEVLIDAGLRHVRTEVRKYRWVYGREEYIDGMSVFASG
                     RFVREMLGASGWDAFRARASAVFAERFPDPLNDFREVILSTAVKV"
     gene            complement(5259..5972)
                     /locus_tag="VIX62_13980"
     CDS             complement(5259..5972)
                     /locus_tag="VIX62_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861385.1"
                     /translation="MSGRTHGLDRRAVGAGLVAAVLLVVVAAISIGEEGGGPTGSPRA
                     SASLAPVTERLTNVGGVYQEEPGIGLDDLDPAELPYPYTRPIPPEAASPIDGAYLKIT
                     PLEGLGQPAVALPIRCLRCIPFRIEAGVSTLILFHGRYFLHHQLSDFRSLGFFAVEGH
                     RVTFSNDPSCPTTHGVYRWRREGIELVLDVIHDPCPFDELRAQDLTASPWTSFNHCLS
                     RFEGLWPGELGCEKRRFQP"
     gene            complement(5969..6700)
                     /locus_tag="VIX62_13985"
     CDS             complement(5969..6700)
                     /locus_tag="VIX62_13985"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY5861386.1"
                     /translation="MADAERPASEPRTGPVLAVFAHPDDAEISVGGTIAKWCAAGREV
                     HLLVLTNGDRGSSDPAQDREDLARIRYTETVAAGEVLGLASVRILSIHDGELENTDDV
                     REAVTRRVREVRAETLLSCDPTLIFFRDSYYNHSDHRTAGFIALDSVFPGSGNPHFHS
                     EHLGEGLEIQEVTDVWLGWTDEPNHVEDISEHFRAKVDALAEHASQLAEGIRFFEEFL
                     AEDAVKAGEKIGVTHAEEFRVLDLR"
     gene            complement(6711..>6899)
                     /locus_tag="VIX62_13990"
     CDS             complement(6711..>6899)
                     /locus_tag="VIX62_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861387.1"
                     /translation="DRWGGAITAAWFLAEFVGQTPWVHVDIAGPAFTDRAHDLGPRGA
                     TGVPVRTLVRFVLDRAGA"
BASE COUNT         1121 a         2486 c         2334 g          958 t
ORIGIN      
        1 caggggagat cggatgagag ggagtcagtc gccgcccagg ccgtcgagaa acgctcgggc
       61 tgctcgcttc gaaccgagcg cgtacgacac cgcatgtcgc ccgtgcctcg cgacgatctc
      121 gcgaccgcgt tcaacctcgg acccgcggtg gcgcaagatg atccccacca atccccactc
      181 atcgcgcaaa cgtcgccagg ctgagtcttc gcaaccttcg ggcatctcgc cgaccggctt
      241 cgacagccct cgcctgaacg cacgggccgc gcacgtccac cagggtgtct caagcagcac
      301 cacggtgtcc gcgcgttcga gccgaagttc aagcgtgtcg aggtcgttgc cgtctgcgat
      361 ccaggcgtta ccggcaagca cgcgacgctg cgccgctcgc cactcctcct ccgacggcct
      421 gacccagccg ggcttccact cgtacaggtc gaggtggatc accggcaagc cggtcttcgc
      481 cgacagcgct cgcgagaacg tgctcttccc ggatccagcc atcccgatga cgagcacgcg
      541 atggccgatg gccccagctg gatcttccat caggttgcgt ctctcgtgtc tgagttcccg
      601 accgctgggg gcagggggac gccactcacc gtgatcgtgg ccacagcgcc gcgaccgcgc
      661 gacccagcaa gaacccgctc gccacgtcgg aggggtagtg gacgccgacg tagacgcggg
      721 aagccgccac cgatgcgctc accgaggtca gcccggcgct gccgagcccg ccgatgccca
      781 gttcccttgc ggccacggac atgaacgcgg ttacgaccgc gggatgactg ctgggccagg
      841 aggtcccgtg tggtggcgcg atcaggaacc gggtggcctc cgcgtcggcc tcgtagggac
      901 ggggacggtt caccgccttc ttcagcccct ggccgaggag ccaggtagct ccggcggccg
      961 agagcgcgcg cacgccctcg cgtggacgtc ccgatacggc cagcaccccg gcggcagcgg
     1021 cggcggcata cagcgacccc agctcggtca ccccgaggaa gaaccgatcc gcgccgggtc
     1081 cggcatcggt gttgccggcc tcgaagagcg cacggtccag gcgctccccg tcggtggtgc
     1141 ggagcgcggc gtagccgaca ccggccgccg tcgcggcgag cgtcacaccc ttgatcacgc
     1201 ttcgaaggtt cacggcgggc aatcatgcat gatggcggcg acgctcgtca caggaggcgc
     1261 gaagcccatg ctcgaggaca acaccggccg ggatgatttc gatccgggtc aaccgaaggg
     1321 gtcgaaggtc aaggcacgca cgatcctgtt gatcgtggtg gtggtcgcct tcgggctgtt
     1381 cctgctccag aacccgcagg aggtgcggat ctcgttcacg ttcatcgagg tccgcgcccc
     1441 gctgtggctg gcgatcgcgg tggccgggct gctcggcctg gtcgccgggt ggttgatggg
     1501 gcgcttcgag ctgggcaagg ggcggatgaa caagcagcgt tagcgctggg ccatcgattc
     1561 gatctcggcc ttctgcgcca gcaccccgtg acgaagaccc atctgggcgc cacccaccgc
     1621 gttcaggctg gccgccgcga acccaccgag cggacctcgg aaccggtagt cgacctcgtg
     1681 ccgcagcatg ctgcgctcat acggcagcga gccgaccgac gtcgtgacga tcgcatcgag
     1741 caggccgctc aggtggacct tcgagcgcca cggcggttcc cactccaaca cctcggcgcg
     1801 caccgtggag ttcacgccca tgaagcccat caccacggaa tagtgggcgc cgagcttcat
     1861 gccaccctcc ggcagggcca cggacaccac gtggcgatcc cagtgcggga ggttgcgggg
     1921 gtcgctcgcc accgtccaca cctgctcggg cggcgcttcg acctccaccg agaactcgac
     1981 ggtggtcatc cgaccggcgc gctcccgctg gaccaaccct tccaccagac caggcgcgag
     2041 ggagccaggc gcacgagcgc gtcagagccc ggatggagtc cggccgccag ggccttcgcg
     2101 gtcttcgcgc ccgaccccag cgaatccatg aggtagatcg agccttcgcc ctgcaccatc
     2161 gcgcccacca tgtctcgcgc tcgccattcg ctgacttgat cgatcgtgag cgccatcgcc
     2221 gcgttgggcc cggccccggc gagcgcgagc gtttcggcgg ggagcgccac atagagcgcg
     2281 gagggttcgg cgagccaccg cgccggcagc gacaccggcc cgcgcgcgcc ggcgacggtc
     2341 agcacgccct ccccgctgcg cccgagcgag gccgcgatgt cggcaggcaa ctcggcgagc
     2401 ggatccgggc cctgcttgtg cgagcggaac gtcgggtgcg acacggcctc tccgccccag
     2461 cgaccgcgac cctcttggac accgtcctcg tcgagcagcg ccgtccgttc gagatcgatg
     2521 cccacgaaca cccgtcccgg cggcgtccac gccaacggca cctgacgggc atcgagggcg
     2581 tagcccgcga agaatcgagc gttcttcttc gagaacttcg cggtggcccg cgcgatcgag
     2641 gtggcgcctg cgaccgccgc gccccaggtt ccgcggtcca gcgcgtcgta ggtcttgacg
     2701 tggccggtga aggtcacgga cagccccccg gagcggacga gccccgagac gctggggtcc
     2761 accttccacg cgcgggtctt caccgagcca cgagacgtgg tgagccagat gcgtccgccc
     2821 gaggccgcga acaccagcgg ggtgcagtgc ggaccgcgcg gcgtcgcggt ggccacggca
     2881 cagaacggcc cttcgtccaa gacctgctgg accgcggtcg acaggaagct cacgcgttca
     2941 cctctctccg gtctgagcgt cggaacggaa ccggaacctg ggtcggccgg ctcccggcgg
     3001 cagcgcgagc atcaaccccg tccacaggat atggctcgcg agactcgcca gcacgccgcc
     3061 cgaccaccag gcgagccccg tccacagcgc cccgcccacc acggcgcccg ccacgatcgg
     3121 cagcgagcgg ctcgagatgt tggccacgac gtacccgacc caggcggcca ccgcggccgc
     3181 ccccgcagcc atcgccacgg ccaggcgccc ctggacgagc ccccgccaga agagctcttc
     3241 cgacgggacc atgatcacca gggacaacaa cagcgagcgc ctgagcgaca cctcggcggc
     3301 ctcggagtac ttctccacga cgtcgcgccg gaacggctcc caatgcgctg cagcccagac
     3361 gaagacacgg gtgctcagga agagcgcgag gccactcgcc aggccggcgg ccagcgccgt
     3421 cgtcaccgtg acctcggcag cggccaccgg aggccgggcc agcaccgccg ccacccccat
     3481 cgtgccgagc acgatcggca tcaggcgcca gacgtcgcgc ccgcgcgcgg agacgaacca
     3541 ccaccccacg atctcggcga gcagcccggc gaccatgacg gcccactgca tcgctcagcc
     3601 caggaacctg gtcttgaagg cgacctccgc ggtgaaaccc gtcacgaccg cgaggtagta
     3661 gaagccggtg gccgactgca cggcggaggc gcgttcaccc ttgagcgcgg cgcgcgtgag
     3721 caccgcgatc aacgccgtcg ttcccaccat gcccagcgcg atccacggcg cgagcgcgcc
     3781 ggccgtgagc aacgggttga gcgagccgga gtcggcggtg gccgcgaacc cgcccgagat
     3841 gaccgcgacc gcctcgatgc ccaccgcgat cagcatcgcc gtcgtgtacc ggttgatcgg
     3901 tcctcgaggc aatccccggt ccgtgaggta ccagtggccc agcagcagcc cgtccgtgat
     3961 ggttcccagg aacgccgcgc ccgccagcag ctggaacagc gagagcgcgt aggcctgccg
     4021 gccggtaccc gccatggccg cgagcacggc gatcgacagg ggcacgctga gcagtcccac
     4081 caccctggcc accgtctgct gcttggccat catcagcagg aacgtcacgc ccaccgcgat
     4141 cgccgtggcg agcgcgagcc gcaccgacca gagcccggcg ttctcgcccg gcaccagggc
     4201 gaccttcgcc gaccaccacg cggcgacggc cagcaccagg aggatcgagg acgtgagggt
     4261 gaagaacccg cgcttgactt cgttccacaa cggcacgcac cacaggaacg ccagcgagcc
     4321 ggcgaccgcc tccgccagca cgagcgccat gtaccctgcc gcgccggtca cctgttcgca
     4381 tcccgcatac ggctcgacag cctacccggc acccgggcgc ggcttcgttg gcggcgacgc
     4441 ccggtaggct gacgggccac atggacgatt ggcgcagcta cgacgacgtc gccgagacct
     4501 acgaacgcgt gcatgcgtcg cggttcgtcc aggtcgccga cgacctggtt gcacaggccg
     4561 ggatcggcga aggtcacctc ctcctcgaca tcggcaccgg gaccgggatc gccgccgagg
     4621 cggccacgaa ggtgggcgcc cgcgtcgtcg ggatcgacga atcgatgggc atgttgcacg
     4681 tggcgcgccg tcagcgtccc gccgaggccg tggcggcggc cgaagcgatc gacctgccgt
     4741 tccgcgatgc ggtgttcgac gccgcgatcg gcagcttcgt gctggcgcac ttcacgaagt
     4801 acgagaccgc gatcttcgat gcgatgcgcg tgttgaaggt cggaggcacg atcgggttct
     4861 cgtcgtggtc cgacggcgtc gacgcctacc agacggcgtg gagcgaactg gtggagtcgg
     4921 tggtgccgcg cgagatcctc gagcctgcct acgccgaagc tcggccctgg cacgacaagt
     4981 tccgcaagcg cgaccagatc gaagaggtgt tgatcgacgc cggcctccgc catgtgcgca
     5041 ccgaggtccg caagtaccga tgggtgtacg gccgcgagga gtacatcgac gggatgagcg
     5101 tgttcgcctc gggccggttc gtgcgcgaga tgctgggcgc ctccggctgg gatgcgttcc
     5161 gggcgagggc ctcggccgtg ttcgccgagc gcttccccga tccgctgaac gatttccgcg
     5221 aggtcatcct gtcgaccgcc gtcaaggtgt gaaccgcatc acggctggaa ccgtcgcttc
     5281 tcacagccga gctcgccggg ccacaggccc tcgaaccgcg agaggcagtg gttgaacgag
     5341 gtccatggcg acgccgtgag gtcctgggcc ctgagctcgt cgaacggaca cggatcgtgg
     5401 atcacgtcga ggacgagttc gatgccctcg cggcgccacc ggtagacgcc atgcgtcgtc
     5461 gggcagctcg gatcgttcga gaacgtcacg cggtggccct cgacggcgaa gaatcccagc
     5521 gatcggaaat cggacagctg gtggtgcagg aagtaccggc catggaacag gatcagcgtg
     5581 ctgaccccgg cttcgatgcg gaaggggatg cagcgcaggc agcggatcgg cagcgcgacc
     5641 gccggttgtc cgagaccttc gagcggcgtg atcttcaggt atgccccgtc gatcggtgat
     5701 gctgcttccg gcgggatcgg acgggtgtag ggatagggca gctcggcagg gtcgagatcg
     5761 tcgaggccga tgcccggttc ctcctggtag acgccgccga cgttcgtgag tcgttcggtc
     5821 accggtgcca gtgaggcgga ggcgcgcggc gagccggtcg gtccgcctcc ctcctcgccg
     5881 atcgagatcg cggccaccac gaccagcagc acggcggcga ccagaccggc gccgaccgct
     5941 cgccgatcga gcccgtgggt gcgtccgctc accggagatc caggacgcgg aactcctcgg
     6001 cgtgcgtgac gccgatcttc tcgccggcct tcaccgcgtc ctcggccagg aactcctcga
     6061 agaaccggat cccctcggcg agctggctcg cgtgttcggc gagggcgtcc accttcgcgc
     6121 ggaaatgctc gctgatgtcc tcgacgtggt tcggttcgtc cgtccacccg agccagacat
     6181 cggtcacctc ctggatctcc aggccctccc cgagatgttc cgagtggaag tgtgggttgc
     6241 ccgaacccgg gaacaccgag tcgagcgcga tgaacccggc ggtgcgatgg tcggagtggt
     6301 tgtagtagct gtccctgaag aagatcagcg tcggatcaca ggacaggagc gtttcggctc
     6361 ggacctcgcg gacacgccgg gtcacggcct cgcggacgtc gtcggtgttc tccagctcgc
     6421 cgtcgtggat gctgaggatc ctgacgctcg ccaacccgag gacctcgccc gcggccaccg
     6481 tctcggtgta gcggatgcgc gcgaggtcct cacgatcctg cgcggggtcg ctcgagccac
     6541 gatcgccgtt cgtcagcacc agcagatgga cctcgcggcc ggcggcgcac cacttcgcga
     6601 tcgtgccgcc gaccgagatc tccgcgtcgt cgggatgggc gaagaccgcg agcaccgggc
     6661 cggtgcgcgg ctccgatgcc gggcgttcgg cgtcggccac aacccgcccc ttacgctccg
     6721 gcgcggtcca gcacgaaccg caccagggtg cgcacgggca cgcccgtggc cccacgcgga
     6781 ccgagatcgt gggcgcgatc ggtgaacgcc ggccccgcga tgtccacgtg cacccacggc
     6841 gtctgcccga cgaactcggc caggaaccag gccgccgtga tggcaccgcc ccagcggtc
//
LOCUS       DATDBZ010000601         4721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1431875, whole genome shotgun sequence.
ACCESSION   DATDBZ010000601 DATDBZ010000000
VERSION     DATDBZ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431875"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1456
                     /locus_tag="VIX62_13995"
     CDS             95..1456
                     /locus_tag="VIX62_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5861388.1"
                     /translation="MQRLAKFAMVIGLAVAVFVTGGIGAFRSLRSEASDGAVNTATTV
                     ATGDLLAPTAGAGQGLGATIESLQQRLAEAPQDWRSFATLGLAYVQQARITSDPSFYP
                     KAQGVLRTSMRLQPQDNEGALVGQAALAAARHDFSAALRYGRQARHVDPYDGNVYGVI
                     ADAQLELGRYDDAFATFQTMVDTLPSLSSYARVSYARELLGDVDGAIRSMEAARDVAG
                     SPADLAWASFQLGELAFNSGRLERASREFERGLSADDSFVPNLAGTAKVAWARGEVDL
                     AIERYAEVVQRYPSPEYVIALGDLYASVGRDEDAQRQFDLVRAEAKLFAANGVDTDLE
                     LALFEADHGDPHAALSAASAEWQRRQSIHVADALAWALHMNGDDIQAARYAERALALG
                     TPNALFLFHAGTIQAALGHDDAARSLLHEALDANPHFSIQHAPEAEALLDDLDSDDVG
                     VAR"
     gene            1453..2898
                     /locus_tag="VIX62_14000"
     CDS             1453..2898
                     /locus_tag="VIX62_14000"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861389.1"
                     /translation="MRRALTLLGLVVAMVLGPVAAASAHPLGNFTINHYSGIHVSPDE
                     IRVDYVIDMAEIPAFQEMEAIDTDGDGEGSDAELAAWAEANAPGLAGGLTLSVDGRDV
                     PLEVRSARAQLLPGQGGLQTLRFEGDFAAAAPAEGTLAYADGNFADLIGWREITAVGE
                     DGTVLEDSSVPTDSVSDALRSYPQDLLASPLDVTAMTASFQPGVSIPGSSPSLDATGG
                     SSARPGVESGPFDALLANQGIGLILLGMVVAVAFGAWHALLPGHGKTLMAAYMVGSKA
                     RKRQAISVGTAVAVMHTASVLALGLLVLSLERTFRPESLYPWLGLVSGLVALALGVYL
                     LISRLSVWGSKGHAHADHDHDHDHDHEQAHDHGLGRHSHALPEGAPLSPRGLIALALA
                     GGILPAPSALLVMLGAINAHRVGYGLALVLAFSIGLATALIVIGMGALKARDLMQQRL
                     SSTMGRLVPVLSAGAIVAVGVFLTARGISQI"
     gene            complement(2902..3609)
                     /locus_tag="VIX62_14005"
     CDS             complement(2902..3609)
                     /locus_tag="VIX62_14005"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY5861390.1"
                     /translation="MTNDHEAIDELLAGYVLRSLSGEDAAEADHLLSDHVPTCAACRD
                     TLAAFQDLSADLALDAVPLAPPETLLPRLHRELEPPASRRRPAQVFAVAASVVAVVGL
                     AGLAVTQGLRASDANSRAEDFRSAAQMLQRPDSNQVSVGPVNELTAPGVEEFYVMGDD
                     CPQPPEGSVYRVWIVAEGQPTYVTDFVPEDGQVFLSIPFDPSRYDDLWISVEPAGSEP
                     TTPTDVEWQASGSAAAA"
     gene            complement(3614..4180)
                     /locus_tag="VIX62_14010"
     CDS             complement(3614..4180)
                     /locus_tag="VIX62_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5861391.1"
                     /translation="MAETDREDLELHRRLLSGDRGAFDELYRRYSPPAYGLAYRLTGQ
                     QILAQDVVHEAFLAVWRAPEAFDPARGAFRSFFLSLVHHRAVDTIRREERLRKRQDRA
                     SNLEPVVDEDVAEDVVDAADMADRRLEVRAALGTLPPEQRQVLEMAYFQGMTQIQIAE
                     QIQIPLGTVKTRTLAAMRKMRRALHRED"
     gene            4224..4457
                     /locus_tag="VIX62_14015"
     CDS             4224..4457
                     /locus_tag="VIX62_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY5861392.1"
                     /translation="MLVRLYARPGCHLCDVARKVIEAERGRTGFTFEEISVEGDDRLE
                     LEYGIRIPVVTIDGRESFEIEVDPEEFAALVRA"
     gene            4546..>4721
                     /locus_tag="VIX62_14020"
     CDS             4546..>4721
                     /locus_tag="VIX62_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HEY5861393.1"
                     /translation="MTRERSIPEATVGRLPVYLRALVETAGSGTATVSSEELAEAAGV
                     NSAKVRKDLSHLGS"
BASE COUNT          694 a         1630 c         1662 g          735 t
ORIGIN      
        1 gccgcgctgc gcgcggccgg gcccgccctc ccgttgacca cgggacgtac cgataggaac
       61 aacgaaggcc gaaggaacga aggagaggag tcggatgcag cgcctcgcga agttcgcaat
      121 ggtgatcggc ctggcggtcg ccgtgttcgt gaccggcggg atcggcgcgt tccgaagtct
      181 gcgctccgag gcatccgatg gcgccgtgaa caccgccacc accgtggcca ccggtgatct
      241 gctggcaccg acggccggtg ccggtcaggg cttgggcgcc acgatcgagt cgctccagca
      301 gcggctggcc gaggcgccgc aggattggcg ctccttcgcc acgctggggc tggcctacgt
      361 gcagcaggcc cgcatcacct ccgatccgag cttctacccg aaggcgcagg gcgtgctccg
      421 cacctcgatg cgcttgcagc cgcaggacaa cgaaggagcc ctggtaggcc aggccgcgct
      481 cgcggccgcg cgtcacgact tctccgccgc cctgcgatac ggccgccagg ctcgccacgt
      541 ggacccctac gatggcaatg tctacggcgt gatcgctgat gcccagctcg aactcggccg
      601 atacgacgat gccttcgcca cctttcagac catggtggac acgctgccgt cgctgtcctc
      661 ctacgcgcgg gtgtcctatg cgcgcgagct gctcggggac gtcgacggtg ccatcaggtc
      721 gatggaagcg gcccgcgatg tcgccggctc gcccgccgat ctggcctggg ccagcttcca
      781 gctcggtgag ctcgccttca acagcggccg gttggagcga gcgtcccgtg agttcgagcg
      841 cggtttgagc gccgacgatt cgttcgtgcc gaacctcgcc ggcacggcca aggtcgcctg
      901 ggcgcgcggc gaggtcgacc tggcgatcga acgctatgcg gaggtcgtcc agcggtaccc
      961 gtcaccggag tacgtgatcg cgctcggcga cctgtacgca tcggtcggtc gcgacgagga
     1021 cgcccagcgt cagttcgacc ttgtgcgagc cgaggcgaag ctcttcgccg ccaacggtgt
     1081 cgacaccgac ctggaactcg cgctcttcga ggccgaccac ggcgatcctc acgccgccct
     1141 ctcggccgcg agcgccgagt ggcagcggcg tcagagcatc cacgtcgccg acgctctcgc
     1201 gtgggccttg cacatgaacg gcgacgatat ccaggcagcc cggtacgccg aacgcgcgct
     1261 cgcccttggc acgccgaacg ccctgttcct gttccacgcg ggcacgatcc aggctgcgct
     1321 gggccacgat gatgcggcac ggagcctgct ccatgaggcc ctcgacgcca acccgcactt
     1381 ctcgatccaa cacgcaccgg aggccgaggc cctcctcgat gacctggact ccgatgacgt
     1441 gggggttgcc cggtgaggcg tgcgctcacg ctgctcggtc tcgtcgtggc catggtcctc
     1501 ggaccggtgg ccgccgcgtc cgcccacccg ctgggcaact tcacgatcaa ccactacagc
     1561 ggcatccacg tgagcccgga cgagatccgc gtcgactacg tgatcgacat ggcggagatc
     1621 cccgccttcc aggagatgga ggcgatcgac accgacggcg acggcgaggg ctcggacgcc
     1681 gagctcgcgg cctgggccga ggcgaacgcc cccgggttgg ccggggggct cacgctctcc
     1741 gtcgacggac gcgacgtgcc gctcgaggtc cggagcgcac gggcgcagtt gttgcccggg
     1801 cagggcgggc tgcagacgct ccggttcgaa ggcgacttcg cggccgccgc gccagctgag
     1861 gggacgctcg cgtacgccga cgggaacttc gccgatctga tcggctggcg cgagatcacg
     1921 gcggtgggcg aagacgggac ggttctcgag gattcttcgg tgcccaccga cagcgtcagc
     1981 gatgccctgc ggtcctaccc gcaggacctg ctggcgagcc cgctggacgt cacggcgatg
     2041 acggcatcgt tccagccggg tgtgagcatt cccggatcca gccccagcct ggacgccacc
     2101 ggcggatcct cggccaggcc cggggtggaa tccggcccgt tcgacgcgct gctcgccaac
     2161 caggggatcg gactgatcct gctgggcatg gtggtggcgg tggcgttcgg cgcgtggcac
     2221 gccctgttgc cgggccacgg caagacgctg atggccgcct acatggtggg atcgaaggcg
     2281 cgcaaacgcc aggcgatctc ggtgggcacg gcggtcgccg tgatgcacac ggcctccgtg
     2341 ttggccctcg ggctgctggt cctgtcgctg gaacgcacgt tccggcctga atcgctgtac
     2401 ccctggttgg ggttggtctc gggtctggtg gccctggcgc tgggcgtcta tctgctgatc
     2461 tcgcggctgt cggtgtgggg gagcaagggc cacgcccacg ccgaccacga ccacgaccac
     2521 gaccatgacc acgagcaggc gcacgaccac ggtctgggcc ggcattccca cgccctgccc
     2581 gagggagcgc cgctgtcacc gcgtgggctg atcgccttgg cgctggcggg cggcatcctg
     2641 cctgctccga gcgcgctcct cgtgatgctc ggcgcgatca acgcccaccg cgtggggtac
     2701 gggctggcgc tggtgctggc gttcagcatc ggcctggcca ccgcgctcat cgtgatcggc
     2761 atgggggcgt tgaaggcacg cgacctgatg caacaacggt tgtcctcgac gatgggccgg
     2821 ctggtgcccg tgctgtccgc gggggcgatc gtcgcggtgg gggtgttcct caccgctcgc
     2881 gggatctcgc agatctgagc cctacgccgc cgcggccgac ccggaggcct gccattcgac
     2941 gtcggtgggg gtggtgggct ccgaacccgc cggctccacc gagatccaca gatcgtcgta
     3001 gcggctgggg tcgaagggga tcgacaggaa cacctgcccg tcctccggga cgaagtcggt
     3061 cacgtacgtg ggttggccct cagcgacgat ccacacccgg tagaccgagc cctcgggtgg
     3121 ctgagggcag tcgtcgccca tcacgtagaa ctcctccacg cccggcgcgg tgagctcgtt
     3181 gaccggtccc accgacacct ggttcgaatc tggccgttgc aacatctgcg ccgcggaacg
     3241 gaagtcttcg gctcgggagt tcgcgtcgct cgcccgcagg ccttgcgtga cggcgagccc
     3301 ggcgagcccg accaccgcga cgacgctggc ggccacggcg aacacctgcg cgggtcgccg
     3361 gcgcgatgcc gggggctcga gctcccggtg caaacgtggc agcagggtct cgggcggagc
     3421 gagcgggacg gcatcgagcg cgaggtcggc cgacagatct tggaacgccg ccagggtgtc
     3481 gcggcaggcc gcacacgtgg ggacgtgatc ggagagcagg tggtcggctt cggcggcgtc
     3541 ctcgcccgag aggctccgca ggacgtagcc ggccagcagc tcgtcgatcg cttcgtggtc
     3601 gttggtcacg cggtcaatcc tctcggtgga gcgcgcgccg catcttgcgc atcgccgcca
     3661 gggttctcgt cttcacggtg ccgagcggga tctggatctg ctcggcgatc tgtatctggg
     3721 tcatgccctg gaaatacgcc atctccagga cctgccgttg ttccggggga agcgtcccga
     3781 gtgccgcacg gacctcgagg cgtcgatcgg ccatgtcggc cgcatcgacc acgtcctccg
     3841 ctacatcttc gtcaaccacg ggctccaggt tcgaagcccg gtcctgtcgt ttgcggaggc
     3901 gctcctcgcg ccggatcgtg tcgaccgccc gatgatgcac cagggacagg aagaacgagc
     3961 ggaacgctcc ccgggccggg tcgaaggcct cgggggccct ccacaccgcc aggaaggcct
     4021 cgtggaccac gtcctgggcc aggatctgct gaccggtgag ccggtaggcc aggccgtagg
     4081 cgggcggcga ataccgccgg tagagctcgt cgaaggcccc ccggtcgccc gacaacaggc
     4141 gacggtggag ctcgagatcc tcccggtcgg tctcggccat gggctcatcc tcccatcgcg
     4201 gcggccccgg ctaccatcgg ggcatgctcg tccgtctcta cgcccgcccc ggctgccacc
     4261 tgtgcgacgt ggcgcgcaag gtcatcgagg ccgaacgcgg acggaccggc ttcacgttcg
     4321 aggagatcag cgtcgaaggg gacgatcgcc tggaactcga gtacgggatc cggatcccgg
     4381 tggtgacgat cgacgggcgc gagagcttcg agatcgaggt cgatccggaa gagttcgcgg
     4441 ccctggtccg tgcgtgacag cggcgcgacg tcgaaccgct tgtgagtttg tgaatcccgg
     4501 ggtccgaacg ctaggctacg gatccatgca gccggagagc cagccgtgac cagggagcgc
     4561 tcgatcccgg aagcgaccgt cggccggctc cccgtctacc tgcgtgccct ggtggagacg
     4621 gcgggttcgg gcaccgccac cgtctcgtcc gaggaactcg cggaggcggc gggcgtcaac
     4681 tccgccaagg tccgcaagga cctctcccat ctgggctcct a
//
LOCUS       DATDBZ010000602         6372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2443264, whole genome shotgun sequence.
ACCESSION   DATDBZ010000602 DATDBZ010000000
VERSION     DATDBZ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443264"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VIX62_14025"
     CDS             <1..568
                     /locus_tag="VIX62_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5861394.1"
                     /translation="MTAVVATASYLRRNHPNARAFVLSDGDGTGDLDGVDVVATPEAA
                     DVMVIGGASEEFSYDIVNRIFRGIRDGAALVGMHRNLYWRTSEHWELDAGAYLAGLEA
                     ATGVTAAICGKPAAAYFDAALSLMGVPAERALMVGDDIVNDIVGAQAMGLTGVLVRTG
                     KFTPASLELGEPDHVIDSLGELPASMRG"
     gene            575..1303
                     /locus_tag="VIX62_14030"
     CDS             575..1303
                     /locus_tag="VIX62_14030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5861395.1"
                     /translation="MADSDRSRAPVIFTGIAPQYSWMGAVMSFGQDGRWRRFMVSKVN
                     AVPGSLVLDVAAGTGLVSRELAARKRLRVVSFDATEPMLRSGLPANELAGLGAQITPV
                     LGRAERLPFPDETFDALTFTYLFRYVDDPAAAMRELARVVRPGGTIATLEFHVPEQPL
                     LRAGWYAYTRGALPVIGSLAGRAWRETGRFLGPSISRFDERAPLPEQVRWWQEAGVRH
                     VRSRVMSLGAGVVVWGVKGGRGVR"
     gene            1293..2018
                     /locus_tag="VIX62_14035"
     CDS             1293..2018
                     /locus_tag="VIX62_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861396.1"
                     /translation="MSDERTAFYALTPGGWRDYWTLLHPPYTVWHLSYVAIGAALAPV
                     LHVGWWLETTAAFFLAMGCAAHALDELHGRPLGTRIPDRVLWAIAVVGLAGAVALGIH
                     GAAEVSPWIWAFIVTGVFLVVAYNLELWGGVVHSDVWFALAWGAFPVLTAYFAQTATI
                     SVSAVLAALACAVISGAQRALSTPVRDLRRRVREVDGTIERNDGTVSPIDRSTLRAAP
                     EAALRLLSFAMPLLAAAMIVPKL"
     gene            complement(2035..3963)
                     /locus_tag="VIX62_14040"
     CDS             complement(2035..3963)
                     /locus_tag="VIX62_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEY5861397.1"
                     /translation="MSETDPILSRLNAEQRQAAEAVRGPVAILAGAGTGKTTTITHRV
                     AHQVRSGAFPASKLLAVTFTEKAANELKERLATLGVEGVEARTFHAAALSQLRKLWPR
                     CMGSDLPDVLDSKAPMIASLANGLPPPHKFMPRSEIAGEIEWAKNRMVAPAGYLDELE
                     RTGHVPPIPPEFMEPIYQGYERRKERAERIDFEDMLGYAVRMYTESAQAAGFVRDRFH
                     AFTVDEYQDVNPLQAALLDAWLGDRDELCVVGDDYQTIYSFTGASPEHLLTFPTRYPD
                     ATVVRLQDNFRSSPQVLAVANALVPTLGGFEKLLRATRPDGPNPTARALPDAEAEVAF
                     VVGEVRRLHETGVAHEEMAVLYRINARSEPFEEAFAAASIPYQVRDGSFLRRPGPRSV
                     LAKLRNASSQDAVAAVEAATDAVGFDANAQPDDTEEVTRQADLARLRALAAEYRDATG
                     SGDVAGYVAELTGRFSTQQSGRGVTLLTFHRSKGLEWDAVFLPRLLDKELPFRTPRSS
                     ADPDEERRLFYVGITRARQHLYVSWPQEARTAPSPFLEEIGVAKPEPAPAKRAARAPV
                     SVSSGGGPLFDRLKEWRRKRASSDGVPAYVVFHDKTLAEIAERRPHDWADLAAVPGIG
                     PAKLDRYADEVLAIVAGG"
     gene            4129..4539
                     /locus_tag="VIX62_14045"
     CDS             4129..4539
                     /locus_tag="VIX62_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY5861398.1"
                     /translation="MALVEDRPPADLMGRVPLHYAALFGDLARAQELIGDGADVHIGD
                     HDAQTPLHYAAQGCSISVAELLLANGAQVDAEDIDGNTPLWNATLRTRGDGPIVQLLI
                     GRGADPRHRNHEDRTPAELAKALGGAELDLHAAG"
     gene            4596..6005
                     /locus_tag="VIX62_14050"
     CDS             4596..6005
                     /locus_tag="VIX62_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY5861399.1"
                     /translation="MTERPRTLGELRAADYPDRTVKQELRGNLLARLGDGQPLFPGVV
                     GFDDSVLPALERGILAGHDLILLGERGQAKTRLVRAMVNLLDEFVPVIAGTETNDHPF
                     RPISAHGKALVAEHGDATELTWIAREDRYAEKLATPDTSVGDLIGDVDPIKVAEGRYL
                     GDELTIHYGMIPRTNRGIVAINELPDLPERIQVSLFNILEERDVQIRGYRVRLDLDML
                     LVATANPDDYTHRGRIVSPLKDRFGTQVRTHYPDTVREEIRIMDQEVTVPAGGVPVRV
                     PTFMKEVVAALTSELRRSPHVNHRSGISVRYSIGNLETLQAAAVRRAARTGEPEAVPR
                     VVDLPAVLMSSEGRVEFDTIEEGRETEILLRALRTAELEVFRRRLSGYDFSGLLHRFD
                     EGLCVDTGELTPAGDLLATVGDLPGLAKLLDRLGVEEESPGVAASAVEFAMEGLYLSR
                     RLNKDGGDGAARYTGADAS"
     gene            6015..>6372
                     /locus_tag="VIX62_14055"
     CDS             6015..>6372
                     /locus_tag="VIX62_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861400.1"
                     /translation="MSDVHEVVGFLVVGVFAVGWIWGLGAWIARRGPGDRYWTWLTVA
                     QVVAGLQAVLGIILLLIGKRPSTWLHLVYGFGPFVILAIAHALARDLQKTQPGKKPIP
                     AWVPFAFGAFICFGLTL"
BASE COUNT          881 a         2183 c         2267 g         1041 t
ORIGIN      
        1 catgacggcg gtggtggcga ccgccagcta cctgcggcgc aaccatccca acgctcgcgc
       61 gttcgtcctg agcgacggtg acgggaccgg cgacctcgac ggcgtcgacg tggtcgcgac
      121 gcctgaagcg gccgacgtga tggtgatcgg cggtgccagc gaggagttct cctacgacat
      181 cgtgaaccgc atcttccgcg gcattcgcga cggcgcggcg ttggtcggga tgcatcgcaa
      241 cctgtactgg cgcacctccg agcactggga gttggacgca ggcgcctatc tcgcagggct
      301 cgaggccgcc accggggtca ccgccgcgat ctgcgggaag ccggcggccg cgtatttcga
      361 cgccgcgttg tcgttgatgg gcgtgccggc cgaacgtgcg ctcatggtgg gcgacgacat
      421 cgtgaacgac atcgtcggtg cgcaggcgat gggcctcacc ggcgtgctcg tgcgcaccgg
      481 gaagttcacg cccgcctcgc tcgagctcgg tgagcccgac cacgtgatcg attccttggg
      541 cgagcttccc gcatcgatgc gaggctgacc gtccatggct gattccgacc ggtcgcgcgc
      601 gcccgtcatc ttcacgggca tcgctccgca gtactcgtgg atgggtgcgg tgatgtcgtt
      661 cggtcaggac gggcggtggc gccgcttcat ggtgtccaag gtgaacgcgg tccccgggtc
      721 gctggtgctc gacgtggcgg cgggaacggg gttggtgtcg cgcgaactcg ctgcgcgcaa
      781 acggctgcgg gtggtctcgt tcgacgccac cgagccgatg ctgcgctcgg ggctccccgc
      841 gaacgaactc gccggtctcg gcgcacagat cacgccggtg ctcggtcgcg ccgagcggtt
      901 gccgtttccg gacgagacct tcgatgcgct gacgttcacc tacctgttcc ggtacgtgga
      961 cgatccggcg gccgcgatgc gcgagctggc tcgggtggtc cgccccggtg ggaccatcgc
     1021 caccttggag ttccacgtgc ccgaacagcc gctgctgcgg gcaggctggt acgcctacac
     1081 gcgcggcgcg ctgccggtga tcgggtcgct ggcgggacgc gcgtggcgcg agaccggccg
     1141 gttcctcggg cccagcatca gccggttcga cgagcgtgcg ccgctcccgg agcaggttcg
     1201 ctggtggcag gaagccggcg tgcgccatgt gcggtcgagg gtgatgtcgc tgggcgcggg
     1261 tgtcgtggtg tggggcgtga agggcggccg cggtgtccga tgagcgcacg gcgttctacg
     1321 cgctgacgcc cggtggctgg cgcgactact ggacgctgct gcacccgccg tacaccgtgt
     1381 ggcacctgtc ctacgtcgcg atcggcgcgg cgctcgcgcc cgtcctgcac gtcgggtggt
     1441 ggctcgagac cacggcggcg ttcttcctgg cgatgggctg cgcggcccac gccctcgacg
     1501 agctgcacgg gcgcccgttg ggcacccgca tccccgatcg ggtgctgtgg gcgatcgcgg
     1561 tggtggggct cgcgggagcg gtggcactgg ggatccacgg cgccgccgag gtctctccgt
     1621 ggatctgggc gttcatcgtg acgggggtgt tcctcgtggt ggcgtacaac ctggagctct
     1681 ggggtggcgt ggtgcactcc gacgtgtggt tcgcgctggc ctggggggcg ttcccggtgc
     1741 tgaccgcgta cttcgcccag acggcgacga tcagcgtgag cgcggtgctc gccgccctcg
     1801 cctgtgccgt gatctccggc gcgcaacgcg cgctgtcgac cccggtgcgc gatctccgtc
     1861 ggcgggtgcg tgaggtggac ggcacgatcg agcggaacga cgggaccgtg tcaccgatcg
     1921 atcgatctac gttgcgggcc gctcctgagg ccgcgctccg gctgctctcg ttcgcgatgc
     1981 cgctgctcgc cgccgcgatg atcgtgccga agctgtgacc cggtcgggcg gcgcttaccc
     2041 gccggcgacg atcgccagca cctcgtcggc gtagcgatcg agcttggcgg gtccgatccc
     2101 cgggacggcg gccaggtcgg cccaatcgtg tggccggcgc tcggcgatct cggccaacgt
     2161 cttgtcgtgg aacaccacgt aggcgggcac gccgtcgctg gaggcgcgct tcctccgcca
     2221 ctccttcaac cggtcgaaca acggaccgcc gcccgagctc accgataccg gggcacgtgc
     2281 ggcgcgtttc gcgggggctg gttccggctt ggcgaccccg atctcctcca ggaacgggct
     2341 cggggccgtt cgagcctcct gcggccagga aacgtagagg tgctggcgcg cccgggtgat
     2401 gccgacgtag aagagccggc gttcctcgtc gggatcggcg ctcgagcgcg gcgtccggaa
     2461 aggcagttcc ttgtcgagca gccggggcag gaacaccgcg tcccattcca atcccttgga
     2521 gcgatggaac gtcagcaggg tcacgccgcg cccgctctgc tgggtggaga accgccccgt
     2581 cagctcggcg acgtagccgg cgacatcccc actgccggtg gcatcgcgat actcggcggc
     2641 gagcgcccgg agccgggcga ggtcggcctg gcgggtgacc tcctcggtgt cgtccggttg
     2701 ggcgttcgca tcgaagccca cggcgtcggt cgccgcttcc accgccgcga cggcgtcctg
     2761 ggacgaagcg ttgcgcagct tcgccagcac cgatcgaggg cccggccggc gcaggaacga
     2821 accgtcgcgc acctgatagg ggatgctcgc cgccgcgaac gcttcctcga acggttccga
     2881 tcgcgcgttg atgcggtaca gcacggccat ctcctcgtgc gccacgccgg tctcatggag
     2941 ccggcgaacc tcgcccacca cgaacgcgac ctccgcctcg gcgtcgggca gcgcgcgcgc
     3001 tgtcgggttg ggtccgtccg gtcgcgtggc tcgcaggagc ttctcgaacc cgccgagcgt
     3061 ggggaccagg gcgttcgcca cggccagcac ctgtggcgag gaccggaagt tgtcctggag
     3121 cctgaccacc gtggcatcgg gataccgcgt ggggaacgtc aacaggtgct ccggcgaagc
     3181 gccggtgaac gagtagatcg tctggtagtc gtcgccgacc acgcagagct cgtcgcgatc
     3241 gccgagccac gcgtcgagca gcgctgcctg gagcgggttc acgtcctggt actcgtccac
     3301 cgtgaacgcg tggaaccggt cgcgcacgaa gccggccgcc tgggccgact cggtgtacat
     3361 gcgaaccgcg tagcccaaca tgtcctcgaa gtcgatgcgc tccgcgcgct ctttgcgccg
     3421 ttcgtatccc tggtagatcg gctccatgaa ctccggcggg atcggcggca catggccggt
     3481 gcgctcgagt tcgtcgaggt agccggccgg ggcaaccatg cggttcttcg cccattcgat
     3541 ctcgcccgcg atctcgctgc gcggcatgaa cttgtgtggc ggtggcaggc cgttggcgag
     3601 cgaggcgatc atcggggcct tcgagtcgag cacgtcgggg aggtcgctgc ccatgcagcg
     3661 gggccacagc ttgcgcagct gcgacagcgc ggccgcatgg aacgtccggg cctccacgcc
     3721 ttcgacaccc agcgtggcga gccgctcctt caattcgttg gccgccttct cggtgaacgt
     3781 gaccgcgagg agcttcgagg cggggaacgc gcccgatcgc acctgatggg cgacgcggtg
     3841 cgtgatggtg gtggtcttgc ccgtgccggc cccggcgagg atggccacgg gcccccggac
     3901 cgcctcggcc gcctgtcgtt gttcggcgtt cagccgcgac aggatcgggt cggtctcgct
     3961 cacggctgga cggtagcagc aggctgggac aaccagttgc ccgacatcct tgcagcggcc
     4021 cacttccggt gcttttctct ctccccgtgg acccttcccc acacgtgttc acgcccatcg
     4081 ggcgaaaatc cgtttcagac ctggccgcgg gcggtcgata ggaagcctat ggctttggtt
     4141 gaggaccggc cccccgcgga cctcatggga cgcgtaccgc tgcactatgc agcgctgttc
     4201 ggggacctcg cgcgggcgca agaactgatc ggcgacggtg ccgacgtcca catcggcgac
     4261 cacgatgcgc agacgccgct gcactatgca gcgcaaggat gctcgatctc cgtcgccgag
     4321 ctgctgctgg ccaacggggc ccaggtggac gccgaggaca tcgacggcaa cacgccgctg
     4381 tggaacgcca ccctccgcac ccggggcgac ggtccgatcg tccagctgct gatcggccgc
     4441 ggcgctgatc cgcgccaccg caaccacgag gaccgcacgc ctgcggagct ggcgaaagcc
     4501 ctgggcggcg ccgaactcga cctgcacgcc gccggctgac ggacggtccc ggccctcgga
     4561 cagccttatc gcggtgagcc gctaccctgg gctgcatgac cgagcgacct cgcaccctcg
     4621 gtgagctgcg cgccgccgac taccccgacc gaaccgtcaa gcaggagctc cgcgggaacc
     4681 tgctggcccg gctcggcgac ggccaaccgt tgttccccgg cgtcgtgggc ttcgacgact
     4741 cggtgctgcc ggccttggaa cgggggatcc tcgcgggtca cgacctgatc ctcctgggcg
     4801 agcgaggcca ggcgaaaacg cgcttggtac gagcgatggt caacctcctt gacgagttcg
     4861 tccccgtgat cgccgggacc gagaccaacg atcacccctt ccggccgatc agcgcccacg
     4921 ggaaggccct ggtggccgag cacggcgacg cgacggagct cacctggatc gcccgcgagg
     4981 accggtacgc cgagaagctc gccacccccg acacgagcgt cggcgacctg atcggcgatg
     5041 tcgacccgat caaggtggcc gagggccgct acctgggcga tgaactcacg atccactacg
     5101 gcatgatccc tcgcaccaat cgcggcatcg tggcgatcaa cgagttgccc gacctgcccg
     5161 agcggatcca ggtgtccctg ttcaacatcc tggaggagcg cgatgtccag atccgcgggt
     5221 accgcgttcg gctcgacctc gacatgctgc tggtcgccac cgcgaacccg gacgactaca
     5281 cgcatcgcgg caggatcgtc tcaccgctga aggatcgatt cgggacgcag gtgcggaccc
     5341 actaccccga cacggtgcgc gaggagatcc gcatcatgga tcaggaggtc acggtgccgg
     5401 ccgggggcgt gcccgtgcgg gtgcccacct tcatgaagga ggtcgtggcg gccctcacat
     5461 ccgagctccg ccgatcgccg cacgtgaacc atcgcagcgg gatcagcgtg cgctactcca
     5521 tcggtaacct cgagacgctg caggccgccg ccgttcgccg cgcggcgcgg accggcgagc
     5581 cggaagccgt gccacgcgtc gtcgacctgc ccgccgtcct gatgtccagc gagggtcgcg
     5641 tggagttcga cacgatcgag gaaggccgtg agaccgagat cctgctgcgt gcgctgcgca
     5701 ccgccgagct cgaggtcttc cgtcgccggc tctccggcta cgacttctcc gggctgctgc
     5761 accggttcga cgaagggctc tgcgtcgaca cgggcgaact gacccccgcc ggcgacctcc
     5821 tcgcgaccgt cggtgatctg cccggcttgg cgaaactgct cgaccggctg ggggtcgagg
     5881 aggagagccc cggggtcgcg gcgagcgccg tggagttcgc gatggaaggg ctctacctct
     5941 cacggcggct gaacaaggac gggggcgacg gcgcggcgcg ctacacgggt gccgacgcct
     6001 cgtagtatcc ggccgtgtcc gatgtgcacg aggtcgtggg gttcttggtg gtcggcgtct
     6061 tcgccgtggg ctggatctgg ggcttgggcg cgtggatcgc tcgtcgaggc ccgggtgatc
     6121 ggtactggac ctggttgacg gtggcccagg tcgtggccgg cctccaagcg gtgctcggca
     6181 tcatcctgct gctgatcggc aagcgtccgt ccacctggct ccatctcgtg tacgggttcg
     6241 gcccgttcgt catcctcgcg atcgcgcatg cgctggcgcg tgacctgcag aagacccagc
     6301 ccggcaagaa gccgatcccc gcgtgggtgc cgttcgcctt cggagccttc atctgcttcg
     6361 ggctgacgct tc
//
LOCUS       DATDBZ010000603         2017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_609393, whole genome shotgun sequence.
ACCESSION   DATDBZ010000603 DATDBZ010000000
VERSION     DATDBZ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609393"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /gene="aspS"
                     /locus_tag="VIX62_14060"
     CDS             complement(<1..917)
                     /gene="aspS"
                     /locus_tag="VIX62_14060"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY5861401.1"
                     /translation="MRTHACGELRAADAGAEVTLCGWVAHRRDHGGVTFIDLRDRHGV
                     VQVVFHPEDAPEAHTGAQKLGGEDVVRVTGMVRARPDGMRNPELATGEVEVVAAELEV
                     LNPSETPPFPIEDRIEVGEDLRLTYRYLDLRRPEMTKVLAMRARIVQLMRELMDGLGF
                     VEIETPLLTRSTPEGSRDFLVPSRLQPGTFYALPQSPQQLKQMLMVGGQDRYYQIVRC
                     LRDEASRADRGFEFTQLDVEMSFVTEEDLIALIEPLYARIVQDIAGIEVAVPFPRMTY
                     AEMMQRYGSDKPDLRYGMELVDLGPVFART"
     gene            complement(940..>2017)
                     /gene="hisS"
                     /locus_tag="VIX62_14065"
     CDS             complement(940..>2017)
                     /gene="hisS"
                     /locus_tag="VIX62_14065"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY5861402.1"
                     /translation="TFEDKGGRSVTLRPESTAGVVRAWLDRAQALGAPWRAYAVANEF
                     RHGRPQAGRLREFRQFDVEVLGVEAPGADIEVITMGDRYVRERGLRDVVLHLNSIGDE
                     VCRPAYRAELVAYFEPFRDQLDRDCRERLEKNPLRVFDCKVDGEKDFVLAAPTIADRL
                     CEPCAQHFVAVRAGLDEAGVPYELDPRLVRGLDYYTRTAFEWISGVLAENKAGTINAG
                     GRYDGLAEQLGGPPTPGVGFAMGLDRVLLAMEGEGVPVPAARTPRCFVVAIGDRSQVE
                     GRRLVEILRDAGVPAVRAFEERPLKAQLKQADRSGAAFVAIIGERELAEGVVTLRRLA
                     DGIQKTVPAGEVSRWLTRLDDWIG"
BASE COUNT          313 a          728 c          668 g          308 t
ORIGIN      
        1 gtccgggcga acaccgggcc gagatccacc agctccatgc cgtatcggag gtcaggcttg
       61 tccgatccgt accgctgcat catctcggcg taggtcatgc gcgggaacgg cacggccacc
      121 tcgatccccg cgatgtcctg cacgatccgc gcatacagcg gttcgatcag ggcgatcagg
      181 tcctcttcgg tgacgaagct catctcgaca tcgagttggg tgaactcgaa cccgcgatcg
      241 gcgcgcgagg cctcgtcgcg gaggcaacgc acgatctggt agtaccggtc ctggccaccc
      301 accatcagca tctgcttcag ctgttggggc gactgcggca gcgcgtagaa cgtgcccggc
      361 tgcaggcggc tcggcaccag gaagtcgcgc gaaccctccg gggtgctgcg cgtcaacaag
      421 ggggtctcga tctcgacgaa ccccagtccg tccataagct cccgcatcag ctgcacgatg
      481 cgggcacgca tcgccagcac cttcgtcatc tcgggccggc gcaggtcgag gtagcggtag
      541 gtgagccgga ggtcctcgcc cacctcgatg cggtcctcga tcgggaacgg cggcgtctcg
      601 gacgggttca gcacctcgag ctcggccgcg acgacctcga cctcaccggt ggcgagttcg
      661 gggttccgca tgccgtcggg acgggctcgc accatgccgg tcacccggac gacgtcctcg
      721 ccccccagtt tctgggcccc ggtgtgcgcc tcgggagcgt cctcgggatg gaacacgacc
      781 tgcaccacgc cgtggcgatc gcgaaggtcg atgaaggtga cgccgccgtg atcgcggcgg
      841 tgtgccaccc agccgcacaa ggtcacctcg gccccggcgt cggcggcacg gagttcgccg
      901 caggcgtggg ttcgcatggt ggttcgcatc gtggtcatct catccgatcc agtcgtcgag
      961 tcgcgtgagc catcgagaca cctcgccggc agggaccgtc ttctggatgc cgtccgcgag
     1021 gcgtcgcagg gtcacgaccc cctcggcgag ctctcgctcc ccgatgatcg cgacgaacgc
     1081 ggcccccgag cggtcggcct gcttcagctg ggccttcaac ggacgttcct cgaaggcccg
     1141 taccgccggt accccggcat cccgaaggat ctccaccaag cgccgacctt cgacctgcga
     1201 gcggtccccg atcgccacca cgaaacagcg aggggtgcga gcggccggca cggggacccc
     1261 ctcaccctcc atggccagga ggacgcgatc cagtcccatg gcgaagccga cccccggcgt
     1321 gggcgggccg ccgagctgct cggcgaggcc gtcgtagcgg ccaccggcgt tgatggtgcc
     1381 ggccttgttc tcggcgagca cgcccgagat ccactcgaac gccgtgcggg tgtagtagtc
     1441 gaggcccctc accaatcgcg ggtcgagctc gtagggcacc cctgcctcgt cgagcccggc
     1501 acggaccgcc acgaagtgct gggcgcatgg ctcgcacaag cggtcggcga tcgtgggcgc
     1561 cgcgagcacg aagtccttct ctccgtcgac cttgcaatcg aacacccgca gggggttctt
     1621 ctccaggcgc tcgcgacagt cgcggtcgag ctgatcgcgg aacggctcga agtaggcgac
     1681 gagttccgcc cggtaggccg ggcggcacac ctcgtctccg atcgagttga ggtgcagcac
     1741 cacgtcgcgc agcccccgtt cgcgcacgta ccgatcgccc atcgtgatca cctcgatgtc
     1801 ggcgcccggg gcttcgacgc ccaggacctc gacgtcgaac tggcggaact cccgcaggcg
     1861 acccgcctgg ggacggccat ggcggaactc gttggcgacc gcgtacgctc gccaaggggc
     1921 ccccagcgcc tgcgcgcgat cgagccaggc tcgcacgact cccgccgtgc tctccggacg
     1981 gagcgtcacc gagcgaccgc ccttgtcctc gaaggtg
//
LOCUS       DATDBZ010000604         4745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_195887, whole genome shotgun sequence.
ACCESSION   DATDBZ010000604 DATDBZ010000000
VERSION     DATDBZ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195887"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..589)
                     /locus_tag="VIX62_14070"
     CDS             complement(119..589)
                     /locus_tag="VIX62_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEY5861403.1"
                     /translation="MGPSEPPCSIAATLDVVGDRWTLLILRDLFRGVHRFSELQNDLG
                     IARNLLTDRLHRLVDHGLVERVEYQQRPVRHEYRLTRKGADLSPALISLMGWGDRWYA
                     DAGPPVELVHDRCGTPIERQPRCPTCDEAVLPGHIRSRPGPGSPTAHLEVIDAS"
     gene            complement(618..1346)
                     /locus_tag="VIX62_14075"
     CDS             complement(618..1346)
                     /locus_tag="VIX62_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861404.1"
                     /translation="MIGQIASAPTGRVEQVHTVGIPDDVGASMTIELDASFDRPNAAF
                     DGEFDFSADPGEDSPLTTWPADVPVGLRVRVVGDQVWAAQGVASDEAPFRDITAAADR
                     ALPVAQLTTIFVLVGLLADAVDIAAPDPVEGVAATWRGLVPPEVLGDIWQEPGPVGSV
                     TRIAGAIPTEYLEGGFVYEVERYDDGRIRLSLWLDAERLTANLGEPMTEDLAHRVDTT
                     WSGLGGPVAIAVPESGSVDESVAE"
     gene            1697..2695
                     /locus_tag="VIX62_14080"
     CDS             1697..2695
                     /locus_tag="VIX62_14080"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY5861405.1"
                     /translation="METTTNERSKRLAVLVARVVLSLAMLVVLFIEIDDVDFSEVVPP
                     LTWSTGLYLGGAMILTFSSIGVSASRWQQVLTALGVHSRWRHLFSYYLAGQFVSNVLP
                     TTIGGDVVRITRLSRETGHGPTSFASVVLERLSGWVVLPLITFLGLALNPGLRELGRA
                     SAVAAGIGLVTLLGLIGVIIVLGDDRILGRFNRSDGWQRFAGAVHLGTTRLRERPASA
                     LAVLCVGVGYQLVLVVAALLAAQALHIADVGFTALLVFMPAVLILQVLPLGISGLGIR
                     EASLVLFLAPLGVPAEQAIALGLLLYLLNLVTSLPGAGALAIGGNRRPPEKSAVLA"
     gene            2692..3564
                     /locus_tag="VIX62_14085"
     CDS             2692..3564
                     /locus_tag="VIX62_14085"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5861406.1"
                     /translation="MTPTDAEVATSTRGALEVAGHEVATSRLRWWREVLYIGAFYIVY
                     TWIRNQFGSASVESFVAYDHALLIIDLERAIGLFHEESIQEFFLDWRLFIQFWNVFYG
                     TFHFVVTIGVLIWLFFRWRPQYAVRRTALAFTTGLALLGFSLFPLMPPRLLCHCEFGA
                     GDGDPHFTDDYGFIDTLDSYGGLWSFNEGAMAKVSNQYAAMPSLHFAWSLWCALAIWG
                     RARSRWAKVLAALYPVATLFAIVVTANHFWLDAVGGAIVLGVGWFLASHLIRWNDRRL
                     GAQVAVADTVTSST"
     gene            3657..>4745
                     /locus_tag="VIX62_14090"
     CDS             3657..>4745
                     /locus_tag="VIX62_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEY5861407.1"
                     /translation="MLLDRIDSPDDLRQMDDRELDQLAGEIRQFIVDAVNASGSGHLG
                     SNLGAVELTLALHRVFDSPRDIILWDTGHQAYVHKIVTGRKDAFVGLRKAGGLSGYPS
                     RDESPHDWIENSHASTVLSYAHGLATAQQSDSGDGRTVVAVIGDGSMTGGMAYEGLNN
                     LGHSGRDCIIVLNDNGRSYAPTVSRLGESLNRIRTNPTYVRETSRLERILRRVPLMGR
                     FLTRGFNATRTAAREMLTEPITFFEQLGLFYSGPFDGHDRREVERALRNAAELGGPQI
                     VHVVTQKGRGYAPAETDNIKRLHDIGPGVAKPGSYTAAFTDAMCKLAEEHPELVAITA
                     AMPDSTGLLPFKDRFPDRFLDVGIAEQHA"
BASE COUNT          721 a         1692 c         1551 g          781 t
ORIGIN      
        1 agtcggttgc cgaaggagca atcgcgcacg gcacgcgccg cggcggtgag gtcgtcggtg
       61 gttcgctctc cgagggcgct tccgacctcg gtgggtgcgg tggggacgag ggtgtggctc
      121 acgatgcgtc gatcacctcg aggtgggcgg tcggagaacc cggaccgggg cggctgcgga
      181 tgtggcccgg gaggaccgcc tcgtcacagg tcggacagcg cggctgacgc tcgatcggtg
      241 tgccgcaccg gtcgtgcacg agctccacag ggggaccggc gtcggcgtac cagcgatcgc
      301 cccagcccat cagcgagatg agcgccggcg acaggtccgc ccccttccgc gtgaggcggt
      361 actcgtggcg cactggacgc tgctggtact ccacccgttc gacgaggccg tggtccacca
      421 gccggtgcag gcgatcggtg agcaggttgc gggcgatccc gaggtcgttc tggagctcgc
      481 tgaaccgatg gacgccccgg aacaggtcgc gcaggatcag cagcgtccac cggtcgccga
      541 ccacgtcgag ggtggcggcg atggagcacg gcggttcaga gggacccatg gtcgagggac
      601 cctagtcaga gtcggtttca ttctgcaacc gactcgtcca cggaccccga ctcaggaaca
      661 gcgatcgcga caggaccccc gaggccactc cacgtcgtgt cgacccgatg tgccaggtcc
      721 tcggtcatgg gctccccgag gttcgcagtc aatcgctctg cgtcgagcca caacgacagg
      781 cggatccgtc catcgtcgta gcgctccacc tcgtacacga agccaccttc caggtactcg
      841 gtcgggatcg cccccgcgat gcgggtcacg cttcccaccg gtccgggttc ctgccagatg
      901 tcacccagca cctcgggcgg aaccaggccc cgccaggtgg ctgcgacgcc ctccacaggg
      961 tcgggtgcgg cgatgtcgac cgcatcggcg aggagcccga cgaggacgaa gatcgtcgtg
     1021 agctgtgcga ccggcagcgc cctgtccgca gcagcggtga tgtcacggaa cggagcctca
     1081 tcggacgcaa ccccctgcgc agcccacacc tggtcgccga cgacacggac cctcaagcca
     1141 acggggacgt ccgcaggcca ggtggtcagc gggctgtcct ccccgggatc ggcagagaag
     1201 tcgaactcgc cgtcgaacgc tgcattgggc cggtcgaagg acgcgtcgag ctcgatcgtc
     1261 atcgacgcac cgacgtcgtc agggatgccc accgtgtgaa cctgctccac acggccggtg
     1321 ggcgccgagg cgatctgtcc gatcacctct ccgacctctg gttcggcggt cctctgctcg
     1381 gctgaggcgc gtggagcttt gttcggcgct gacgccgggg cgcaccccgg gacgagtgcg
     1441 aggacgagca ctgacgtgac aatcagggtc acgggacgat cgtggcgatc cacgaggacc
     1501 tcctggcggc ggggctggag gccgggacca ccggcgagga gtcgcgcgtg cgacgacggg
     1561 tcgaccgcag agtgccagac cgaccgcagg ccggcaaggg ccgcctcagg gccggacctg
     1621 cacgggtcga ccgggtcggc tcgccgtaga ctcccctgac cggatcgcat ggtgtgaccg
     1681 gcagctgcgg agacccatgg agaccacgac caacgagcgg agcaagcgcc tcgcggtcct
     1741 tgtcgcgcgc gtcgtcctca gcctcgcgat gctcgtcgtc ctgttcatcg agatcgacga
     1801 cgtggacttc tccgaggtcg ttccgccgct cacctggagc acgggtctct acctcggcgg
     1861 ggcgatgatc ctcaccttct ccagcatcgg cgtctcggcg tcgcgctggc agcaggtcct
     1921 caccgcactc ggggtgcact cgcggtggcg ccacctgttc tcgtactacc tcgcgggcca
     1981 gttcgtctcg aacgttctcc cgacgacgat cggcggcgat gtcgtgcgga tcacacgcct
     2041 gtcgcgcgag acgggccacg gccccaccag cttcgcctcc gtcgtgctcg aacgcctcag
     2101 cgggtgggtc gtgctcccgc tcatcacgtt cctcggtctc gcactcaacc cgggcctgcg
     2161 tgagctcggc cgggcctccg cggtcgccgc gggcatcggg ctcgtgacgc ttctcggcct
     2221 catcggcgtc atcatcgtgc tgggcgacga ccgcatcctc ggacgcttca atcggtccga
     2281 cggctggcag aggttcgctg gcgccgtcca cctgggcacg acacgactgc gcgagcgccc
     2341 cgcgtcagcg ctcgccgtgc tgtgcgtggg cgtgggttac cagctcgtcc tcgtggtcgc
     2401 tgcgctgttg gccgcccagg cgttgcacat cgccgacgtg ggcttcaccg cgctgctggt
     2461 cttcatgccc gcagtcctga tcctccaggt cctgccgctc gggatctccg gactcgggat
     2521 ccgcgaagcc tccctggtgc tgttcctcgc tcccctcggc gtgccagccg agcaggcgat
     2581 cgcactcggc ctcctcctgt acctgctgaa cctcgtgaca tcgctacccg gcgccggagc
     2641 cctggccatc ggcggcaacc ggcgtccgcc cgagaagtcg gcggttctgg catgacgccc
     2701 accgatgccg aggttgcgac gtccacacgc ggcgccctcg aggtggcggg ccatgaggtc
     2761 gccacgtccc gactccgttg gtggcgagag gtcctctaca tcggcgcgtt ctacatcgtc
     2821 tacacctgga tccgaaacca gttcggctcg gcatcggtgg agagcttcgt cgcctacgac
     2881 cacgccctgc tcatcatcga cctcgagcgg gccatcgggc tgttccacga ggagtcgatc
     2941 caggagttct tcctcgactg gcgcctgttc atccagttct ggaacgtgtt ctacggcacc
     3001 ttccacttcg tcgtcacgat cggcgtcctc atctggctgt tcttccgttg gcgtccccag
     3061 tacgcggtgc gccgcaccgc actggcgttc accaccggcc tcgcgttgct cgggttcagc
     3121 ctcttccctc tgatgccccc acgtctgctg tgccactgcg agttcggagc aggcgacggc
     3181 gacccccact tcacagacga ctacggattc atcgacacgc tggactccta tggcggcctg
     3241 tggtccttca acgagggcgc gatggccaag gtctccaacc agtacgcggc aatgccctct
     3301 ctgcacttcg cctggtcgct ctggtgtgcg ttggcgatct ggggccgggc ccgttctcgt
     3361 tgggccaagg tgctggccgc gctctacccg gtcgccacgc tcttcgcgat cgtcgtgacc
     3421 gcaaaccact tctggctcga cgcggtcggt ggtgccatcg tcctcggcgt cgggtggttc
     3481 ctcgcgtcgc atctcatccg ctggaacgac cggcgtctcg gggcacaggt cgccgtggcc
     3541 gacaccgtga cgtcgtccac ctgatcctcg acatccggcg cgtggatgcg cgcgaccggc
     3601 aggagtacgt tggagcctcg gcccacgggc ctccgcgaag gaaggacccg gagcacatgc
     3661 tccttgaccg aatcgacagc cccgacgacc tgcgacagat ggacgatcgc gagctcgatc
     3721 agctcgcggg ggagatccgt cagttcatcg tcgatgccgt caacgcgagc gggagcggcc
     3781 acctcggatc gaacctgggc gcggtcgagc tgaccttggc gctgcacagg gtgttcgact
     3841 cgccccgcga catcatcctg tgggacaccg gccaccaggc ctacgtccac aagatcgtca
     3901 cgggccgcaa ggacgccttc gtgggcctgc gaaaagcagg aggcctctcc ggctacccga
     3961 gtcgcgacga gtctccccac gattggatcg agaacagcca cgcctccacc gtgctcagct
     4021 acgcgcacgg cctcgccaca gctcagcagt ccgacagcgg tgatggccgc accgtcgtcg
     4081 cggtcatcgg ggatggctcg atgaccggcg gcatggcgta tgaggggctc aacaacctcg
     4141 gacacagcgg gcgcgactgc atcatcgtcc tcaacgacaa cgggcgttcc tacgcaccga
     4201 cggtctcccg cctcggcgag tcgctcaacc gcatccgcac caaccccacc tatgtgcgcg
     4261 agacgagccg gctcgagcgg atccttcgcc gcgtccccct gatgggtcgc ttcctcaccc
     4321 ggggcttcaa cgcgacacgg accgcggcac gggagatgtt gaccgagccc atcacgttct
     4381 tcgagcagct cgggctcttc tacagcggcc cgttcgacgg acacgatcga cgcgaggtcg
     4441 aacgagcgct gcgcaacgcg gccgagctgg gcggacccca gatcgtccac gtcgtcacgc
     4501 agaagggccg cggctacgcg ccggccgaga ccgacaacat caagcggctg cacgacatcg
     4561 ggccgggcgt cgccaagccg ggcagctaca ccgccgcctt caccgacgcc atgtgcaagc
     4621 tcgccgagga gcacccggag ctggtggcca tcaccgcggc gatgcccgac tccacgggcc
     4681 tcctgccgtt caaggaccgc ttccccgacc gcttcctcga cgtgggcatc gccgagcagc
     4741 acgcg
//
LOCUS       DATDBZ010000605         2146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_1225405, whole genome shotgun sequence.
ACCESSION   DATDBZ010000605 DATDBZ010000000
VERSION     DATDBZ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225405"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VIX62_14095"
     CDS             <1..403
                     /locus_tag="VIX62_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014348048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861408.1"
                     /translation="QDTVLVGDAAGLVSPLTAGGIHTALRSGRAAGIAIAGHLLDEGP
                     TPGRALRETYPTFAWKLPMRAAMDLHPPNALIDLALESPRLRAVARAVFFHHRGLLSA
                     EAWRDIRPAATARAGARIPASTARDEKGSSD"
     gene            complement(777..1301)
                     /locus_tag="VIX62_14100"
     CDS             complement(777..1301)
                     /locus_tag="VIX62_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5861409.1"
                     /translation="MNASTETLQTTQVYRVYIKASPDRIWQAITDPSWTARYGYGGTV
                     DYDLRPGGKHIAYTSGAMRATGAPDVAIDGEVVESDPPNKLVLTWRMVMDEAMAAEGF
                     TRLTYEIVEGQGGVSKLTLTHELEGAPQLALLMSGGLEEMGAGGGWSWVLSDLKSLLE
                     TGEPMDAHDGLNQG"
     gene            complement(1298..1624)
                     /locus_tag="VIX62_14105"
     CDS             complement(1298..1624)
                     /locus_tag="VIX62_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5861410.1"
                     /translation="MNDDDRVFKALADPTRRFLLDSLFARDGRTLTELESELEMTRFG
                     VMKHLRVLEDAGIVLTRRQGREKLHFLNPMPIRLIHDRWIDKYTERRVSALADLKHQL
                     EQEGTA"
     gene            complement(1681..>2146)
                     /locus_tag="VIX62_14110"
     CDS             complement(1681..>2146)
                     /locus_tag="VIX62_14110"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY5861411.1"
                     /translation="SIDALEGRRNAEAEHGAVMPPEALQRLACDARVQVVAENEAGDA
                     VAFGRTRREPSAAMLRQLRHRDRGCRFPGCGSTAFANAHHIVWWSRGGTTDLDNLLLI
                     CGFHHRLVHEHGWRVERAFDGRVRWFRADGSRYRGGPELHAGMRPDGFVETG"
BASE COUNT          318 a          761 c          730 g          337 t
ORIGIN      
        1 ccaagacacg gtgctcgtgg gcgacgctgc cgggctcgtc tcgccgctca cggccggcgg
       61 tatccacacc gcgctgcgct ccggacgtgc ggccggcatc gcgatcgccg gccatctgct
      121 cgatgagggc cccaccccgg gcagggccct gcgcgagacc tatccgacgt tcgcctggaa
      181 gttgcccatg cgcgccgcga tggacctgca cccacccaac gccctgatcg acctggccct
      241 cgaaagccct cggctgcggg cggtcgcgcg ggccgtgttc ttccaccatc gagggctgct
      301 gtcggccgag gcatggcgcg acatccggcc cgcggcgacg gcacgggccg gtgcccggat
      361 cccggcatcg acggcgcggg acgagaaggg ttcctccgat tgacaacgcc aggcccggcc
      421 actaggagag aaccaccaca cgggaaggag ctggacacga acgaccggct cgacgcgctg
      481 gaccgtcgca cgtcgaagct tgggggccag ggtctgtact caggcccgat cgacaacgga
      541 cagctcgagt tgggagccga cccggtcgcc tgcgcagggc aggtcgccga gtggaacgtc
      601 gccggcacgt cgctcgggtg cgtgccgacg gctccctagc cggtcggcga catcgagatc
      661 ttggctggac gcgaggcggg ggcggaggcg atcgatgcac ctccaccccc gggcggcctt
      721 ccctcgcaga acatggcccc ggccgttgcg tccttccgtt gcgctgatcc ttggtcctag
      781 ccctggttca agccgtcgtg cgcgtccatc ggctcgccgg tctcgagcag actcttgagg
      841 tcgctcagga cccagctcca gccaccgccg gcgcccatct cctcgaggcc gcccgacatc
      901 agcagcgcca gctgtggggc gccctcgagc tcgtgggtga gggtgagctt cgacacgccc
      961 ccctgcccct cgacgatctc gtacgtcagg cgggtgaagc cctcggcggc catcgcctcg
     1021 tccatgacca tgcgccacgt caggacgagc ttgttcggtg ggtcggactc gacgacctcg
     1081 ccgtcgatcg cgacatcggg cgctccggtc gcccgcatgg ccccgctcgt gtaggcgatg
     1141 tgcttgccgc ccggacgcag gtcgtagtcg accgtgccgc cgtagccata gcgggccgtc
     1201 caggacggat cggtgatcgc ctgccagatg cggtcgggcg aggccttgat gtacacccgg
     1261 tagacctgcg tggtctgcag ggtctcggtg ctcgcgttca tgcggtgccc tcctgttcca
     1321 gctgatgctt cagatcggcg agcgccgaca cccggcgctc ggtgtacttg tcgatccagc
     1381 ggtcgtggat cagccggatg ggcatcgggt tgaggaagtg cagcttctcc ctaccctgcc
     1441 ggcgcgtgag gacgatgccc gcgtcttcga gcactcgcag gtgcttcatg accccgaacc
     1501 gcgtcatctc cagctctgac tcgagctcgg tgagcgtgcg gccgtcacgc gcgaagagcg
     1561 agtcgagcag gaagcgacgc gtcgggtcgg cgagtgcctt gaacacgcgg tcgtcgtcgt
     1621 tcacgggacg aggataggtg accttgtggt cacatgtcaa ccacgaaccc tcgcccccga
     1681 tcagccggtc tcgacgaatc cgtcgggccg cataccggcg tgcagctcgg gaccgccccg
     1741 gtagcgggag ccatcggctc ggaaccacct gacccggcca tcgaacgcgc gctcgacccg
     1801 ccacccgtgt tcgtgcacca accggtgatg gaacccgcag atcaacaaca ggttgtcgag
     1861 gtcggtggtg ccgccgcgcg accaccagac gatgtggtgc gcgttcgcga aggctgtgga
     1921 tccgcagcca gggaagcggc acccacggtc gcggtggcgg agctgtcgga gcatcgcagc
     1981 cgatggctcg cgcctcgtgc gaccgaacgc aacggcgtca ccagcctcgt tctcggccac
     2041 cacttgtacg cgggcatcgc acgcgagccg ctgcagcgcc tcgggaggca tcacggcccc
     2101 gtgctcggcc tcggcgtttc gccggccctc gagggcgtcg atcgag
//
LOCUS       DATDBZ010000606         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2028328, whole genome shotgun sequence.
ACCESSION   DATDBZ010000606 DATDBZ010000000
VERSION     DATDBZ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028328"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VIX62_14115"
     CDS             <1..625
                     /locus_tag="VIX62_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861412.1"
                     /translation="TPVEGGSDRHALTVDADTCTLYELFNARWNGGNPTAGSGAIFDL
                     NSNDLRPAGWTSADAAGLPILPGLVRHDEVLAGYIGHAIRMTSDCTSRNYLWPARHQA
                     GVRDPRCPPMGARFRLKQGFDVSSYSKNAKVVLDAFKHFGLIVADNGSDWFFQGTVDH
                     DWTNGLMDQLKRVPAGAFVAVNEAGCKVSSGSGQYDYGPKCPSPNPA"
     gene            complement(630..1196)
                     /locus_tag="VIX62_14120"
     CDS             complement(630..1196)
                     /locus_tag="VIX62_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEY5861413.1"
                     /translation="MNEQQEAYARAVSDFTEKVHAIGPEQWTNPTPCTEWDVRALVNH
                     LTYENLWVPELIGGRTVAEVGDRFDGDVLGDDPVGAWDRSAEGAIAAVTADGALDRTV
                     DTSGGPTPASDYLDQIFVDAVIHGWDLARGTNTQDRIRVAFADQLFAAYGPREAELRS
                     YGVYGSTVVPPEGADTQTKLLAVFGRVQ"
     gene            complement(1256..1822)
                     /locus_tag="VIX62_14125"
     CDS             complement(1256..1822)
                     /locus_tag="VIX62_14125"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY5861414.1"
                     /translation="MILVVDHYDSFTYNLVQLIESLGHDTQVVKSDAEPAEALAAREP
                     AGVILSPGPGRPEAAGCFTDLLEVLAPEVPVLGVCLGHQAIGVEYGGTVDRTEPVHGK
                     ASKVFHEGRGILEGVNDPFEAGRYHSLVVERDDLPDSLELTAWTEDGLVMATQHRELP
                     RFGVQFHPESILTPEGPTIVRTFLALTT"
     gene            complement(1819..>2115)
                     /locus_tag="VIX62_14130"
     CDS             complement(1819..>2115)
                     /locus_tag="VIX62_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY5861415.1"
                     /translation="KRRAMELIAAHEPDARGPYGGAVGYCGFEGDLDFCITIRTAVVK
                     DGNAHLQSGAGVVADSDPQTELDETKAKASALLPAVAPSEPTRDGTLTAPKERA"
BASE COUNT          319 a          739 c          720 g          337 t
ORIGIN      
        1 gacccccgta gaaggtgggt cggaccgtca cgcactgacg gtcgatgctg acacctgcac
       61 gctgtacgaa ctgttcaatg cacgctggaa cggtgggaac cccacggcgg gtagcggcgc
      121 gatcttcgat ctgaacagca acgacctgcg acccgccggc tggaccagcg ccgacgccgc
      181 ggggctgccc atcctgcccg gactcgtccg ccacgacgag gtcctggccg gctacatcgg
      241 gcacgcgatc cgcatgacgt ccgactgcac cagccggaac tacctgtggc ccgcccgtca
      301 ccaggccggg gtgcgggacc cgcgatgccc gccgatgggc gcgaggttcc ggctgaagca
      361 gggcttcgac gtgtcgagct actcgaagaa cgccaaggtg gtgctcgacg ccttcaagca
      421 cttcgggctg atcgtggccg acaacggcag tgactggttc ttccagggca cggtcgacca
      481 cgactggacg aacggcctga tggatcagtt gaagcgggtc cccgcggggg cgttcgtcgc
      541 cgtgaacgaa gccggatgca aggtgtcctc gggcagcggc cagtacgact acggcccgaa
      601 gtgcccgtcg cccaacccgg cctgaccggt cactgcacgc ggccgaagac cgcgaggagc
      661 ttggtctgcg tgtcggctcc ctcaggcggc acgaccgtgc tgccgtacac gccataggac
      721 cgcagttcgg cttcgcgcgg accgtaggcg gcgaagagct ggtcggcgaa cgccacacgg
      781 atcctgtcct gggtgttcgt gcctcgggca agatcccacc cgtggatcac ggcgtcgacg
      841 aagatctggt cgagatagtc cgaagccggc gtcggtccgc cgctcgtgtc cacggtccga
      901 tccagcgcgc cgtcggccgt caccgccgcg atcgcacctt cggcagagcg gtcccacgcg
      961 ccgacgggat cgtctccgag cacatccccg tcgaagcgat cgcccacctc ggccacggtg
     1021 cggccgccga tcaactcggg cacccacagg ttctcgtagg tcaggtggtt gacgagcgcc
     1081 cgcacgtccc actccgtgca cggggtgggg ttggtccact gctcgggccc gatcgcgtgg
     1141 accttctcgg tgaagtcgga gaccgcacgc gcgtacgcct cctgctgttc gttcacgacg
     1201 cctccctgct cgcgtggggt tcgaccctag cgcgatgcgg gctcttctga cgaggtcacg
     1261 tcgtgagcgc caggaaggtg cgcacgatcg tggggccctc cggcgtcagg atgctctcgg
     1321 ggtggaactg cacgccgaac cgaggcagtt cgcggtgctg ggtcgccatc accagaccgt
     1381 cctcggtcca ggcggtcagc tccaaggagt ccggcaggtc gtcgcgctcc accacgagcg
     1441 agtggtagcg accggcttcg aacgggtcgt tcaccccttc caggatgccc cggccctcgt
     1501 ggaagacctt cgaggccttg ccgtgcacgg gctcggttcg gtcgacggtg ccgccgtact
     1561 ccacgccgat cgcctggtga cccaggcaca ccccgagcac cggcacctcg ggcgcaagca
     1621 cctcgagcag atcggtgaag caccccgccg cctcgggacg ccccggtccc ggggacagga
     1681 tcacgccggc gggctcgcgg gccgcgagcg cctcggccgg ttcggcgtcg ctcttcacca
     1741 cctgggtgtc gtggcccagg ctctcgatca gttgcacgag gttgtaggtg aacgagtcgt
     1801 agtggtcgac gaccaggatc atgcgcgctc cttcggggcc gtcagcgtac cgtcgcgggt
     1861 gggctcggac ggggcgaccg cgggcaggag tgccgacgcc ttcgccttcg tctcgtccag
     1921 ctcggtctgc gggtcggagt cggcgacgac accggccccc gattgcaggt gggcgttgcc
     1981 gtctttcacc accgccgtgc ggatcgtgat gcagaagtcc aggtcgcctt cgaacccgca
     2041 gtagcccacg gcgccgccat acggacctcg ggcgtcgggt tcgtgggcgg cgatcaactc
     2101 catcgcccgc cgttt
//
LOCUS       DATDBZ010000607         3402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_3064301, whole genome shotgun sequence.
ACCESSION   DATDBZ010000607 DATDBZ010000000
VERSION     DATDBZ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3064301"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VIX62_14135"
     CDS             <1..535
                     /locus_tag="VIX62_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY5861416.1"
                     /translation="DSFIVDPHKWLFAPYDCCALLYRDPERARGVFRQEASYLETVNQ
                     EGAAWNEWNPADFAYHLTRRARGLPFWFSLATYGTDAYREAVEAVLALTRETAAEIER
                     HPDLELLMDPELSVVLFRRTGWAGDDYEAWWRRLLDSQIAFVQPTSWQGEKVARLCFV
                     NPRTTIDHVRAVLGTMN"
     gene            603..1523
                     /locus_tag="VIX62_14140"
     CDS             603..1523
                     /locus_tag="VIX62_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861417.1"
                     /translation="MKRISILITMVFTVGALATPATAGLTPQKVVGGPGAQYWPSSNG
                     TFVAWSDGTKRLARIFVMQLSNGHRARVNRPGTDSNLGSFIQGTDELIYQEYSGTRSD
                     LFFYDASTEVRRKAPKTVNAPGFEYWPQGSQRFLLYLRATNRFRTRSLFLYDRASGKQ
                     RRLVDSIGRGRTIYPGFVGDRYAAWSTCNGEYCTISVYDDVTHVVERLPRPSGKADYA
                     PTIDEGTDQLYFVRSRADVCGRGVTIRTEPLGSTASTVVATLPRGIDTGWNTSLTTGV
                     DSGFLDIYFERWDCRRKVSDIYALRSVNAP"
     gene            complement(1510..2412)
                     /locus_tag="VIX62_14145"
     CDS             complement(1510..2412)
                     /locus_tag="VIX62_14145"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5861418.1"
                     /translation="MTPGINRLKTWVLIAGLGGLFVLVGAWIGGSQGAMIALAFGLVF
                     NFAMYWYSDKIAIATTRSKPVTEDEYPQLYRIVRELAQERQMPLPKIFVSQMQQPNAF
                     ATGRSPEHASVAVTEGILAILDERELRGVLAHELSHVLNRDILIGSIAAAIGMSITFL
                     ARFALFFGGGDENRGGGFGVLAAWILAPIAAAIIQMAVSRSREYEADESGAYLSKDPD
                     ALASALRKLEVASKQVPPLRSVSPAEAHMFIVNPMAGLRANGGFGTLFSTHPPTAERI
                     ARLDAIAQEMQGGPALPEPPVRGR"
     gene            complement(2493..>3402)
                     /locus_tag="VIX62_14150"
     CDS             complement(2493..>3402)
                     /locus_tag="VIX62_14150"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY5861419.1"
                     /translation="EIAAKLSTLPAQQETLALAAILFVVVGFAFKVSAVPFQFWAPDT
                     YEGAPVPVAAFLAVASSAAGFAGLLQLMFVAFIGQHEFWVPLFVFLSVATMFVGNLVA
                     LQQTQVVRLLAYSGIAQAGYILLTFALVTDDQASNATAFQAAVVYILIYGIMTIGAFA
                     VAVAVSRRHPTLRLDDFGGLAKTAPILAVGMTVFMISLAGVPPTGGFWAKILIFRAAI
                     DRGGSLGIWLAVLMLVNSVISVAYYFAVPRQMIFKEPADASPLRAPWLVTAVVSVATV
                     AIVALFILPNPFARLAELSTLVGFGT"
BASE COUNT          623 a         1152 c         1112 g          515 t
ORIGIN      
        1 cgactcgttc atcgtcgacc cgcacaagtg gctgttcgcg ccctacgact gctgcgccct
       61 gctgtaccgc gatcccgaac gagcgcgcgg ggtgttccgc caggaagcga gctacctcga
      121 gacggtgaac caggagggcg ccgcctggaa cgagtggaac cccgccgatt tcgcctacca
      181 cctgacgcgg cgcgcccgcg ggctcccgtt ctggttctca ctggcgacct acggcaccga
      241 tgcgtaccgg gaagccgtcg aggcggtcct ggcactcacc cgcgagactg ccgcggagat
      301 cgagcgccat cccgacctcg aactgctcat ggaccccgag ctcagcgtgg tgctcttccg
      361 ccgcaccggg tgggccggcg acgactacga agcgtggtgg cgccgtctgc tggactccca
      421 gatcgcgttc gttcagccga cgtcatggca gggcgagaag gtcgcgcgtc tgtgcttcgt
      481 gaacccgcga accacgatcg accacgtccg cgccgtcctc ggcacgatga actgatcacg
      541 gatctgccct cccggtaagc acttcggtgc cctcgttgat tcatgacggc gaaggaggtc
      601 cgatgaagcg catctcgatc ctgatcacga tggtgttcac cgtgggagcc ctggcgacgc
      661 ctgcgaccgc cggactcaca ccacagaagg tggtcggcgg ccccggcgcc cagtactggc
      721 cgagctcgaa cggaaccttc gtggcgtgga gcgacgggac gaagcgcctg gcgcgcatct
      781 tcgtcatgca gctgtcgaac ggccatcggg ccagggtgaa tcggcctgga accgacagca
      841 acctaggctc gttcatccag ggcaccgacg aactcatcta tcaggagtac tccggaacca
      901 ggtcggatct cttcttctac gacgcgtcga cggaggtccg tcgcaaagcc ccgaagaccg
      961 tgaacgctcc cgggttcgag tactggcccc aaggctctca acgattcctg ctgtacctca
     1021 gggcgacgaa ccggttccgg acacgctcgt tgttcctcta tgaccgtgcc tcgggaaagc
     1081 agcgccgact cgtcgactcg atcggccggg gccgaaccat ctatccagga ttcgttggag
     1141 accgatatgc ggcgtggagc acgtgcaatg gcgagtactg cacgatctcg gtctacgacg
     1201 acgtgaccca cgtcgtggag cgactgccgc gaccgagcgg caaagctgac tacgcgccca
     1261 cgatcgatga aggcaccgac caactctatt tcgtgcgcag tcgagccgac gtctgcgggc
     1321 gaggcgtgac gatccggacc gaacctttgg gctcgacggc ctcgacggtg gtcgcgacgc
     1381 taccgagagg gatcgacacc ggttggaaca catcgttgac gaccggcgtc gacagcggat
     1441 tcctcgacat ctacttcgaa cgatgggact gccgccgaaa ggtgtccgac atctacgccc
     1501 tccggtcggt caacgccccc tgaccggggg ttccggcagg gccggaccgc cctgcatctc
     1561 ctgggcgatc gcgtcgagcc gtgcgatccg ttcggcggtg ggcgggtgcg tcgagaacag
     1621 cgtgccgaac ccgccgttgg cccgcagccc tgccatgggg ttcacgatga acatgtgcgc
     1681 ctcggcgggg ctcaccgacc gcagcggcgg cacctgtttg gacgcgacct cgagcttgcg
     1741 caaggcgctg gcgagcgcgt cggggtcctt cgacaggtag gcgccggatt catcagcctc
     1801 gtattcacgc gaacgtgaga cggccatctg gatgatcgcc gccgcgatgg gggccaggat
     1861 ccacgcggcc aggacgccga acccgccacc acggttctcg tctcccccgc cgaagaacag
     1921 cgcgaacctg gccaggaagg tgatgctcat gccgatcgcg gccgcgatcg agccgatcag
     1981 gatgtcgcgg ttgagcacgt gcgacagttc gtgggcgagg acgccgcgca gctcgcgctc
     2041 gtccaggatc gcaaggatgc cttcggtgac ggccaccgat gcgtgctcgg ggctgcgtcc
     2101 cgtggcgaag gcattgggct gctgcatctg cgagacgaag atcttcggca acggcatctg
     2161 ccgctcctgg gccagctccc gcacgatccg gtagagctgc gggtattcgt cctcggtgac
     2221 cggcttcgag cgcgtcgtcg cgatcgcgat cttgtccgag taccagtaca tcgcgaagtt
     2281 gaacacgagc ccgaacgcca gcgcgatcat ggccccctgg gacccaccga tccatgcgcc
     2341 gacgagcacg aagagcccgc cgagacctgc gatcaggacc caggtcttca accgattgat
     2401 gccgggtgtc acgtcacatc actccctcgt gagaccagcg tctcaccgag ggttcgatac
     2461 gacgagggac acggttcgcc cgttgacggg cgtcaggtgc cgaagccgac caaggtggag
     2521 agctcggcga gccgcgcgaa cgggttcggc aggatgaaca gcgcgacgat ggcgaccgtc
     2581 gcgaccgaga ccaccgcggt caccagccag ggcgcccgca acggcgaggc gtcggccggc
     2641 tccttgaaga tcatctggcg cggcaccgcg aagtagtacg cgacgctgat caccgagttc
     2701 accagcatca gcacggccag ccagatcccg agcgaaccgc cgcggtcgat cgcggcccgg
     2761 aagatcagga tcttggccca gaacccaccg gtgggcggca caccggcgag ggagatcatg
     2821 aacacggtca tgcccacggc caggatcggc gcggtcttcg ccagtccgcc gaagtcgtcg
     2881 aggcgcaagg tgggatggcg ccggctcacc gccacggcca ccgcgaacgc gccgatcgtc
     2941 atgatgccgt agatcaggat gtagaccacc gccgcctgga acgcggtggc gttcgaggcc
     3001 tggtcgtcgg tgaccaacgc gaacgtcagc aggatgtatc cggcctgggc gatccccgag
     3061 tacgcgagca gccgcaccac ctgggtctgc tgcaacgcca cgagattgcc cacgaacatc
     3121 gtcgcgaccg acaggaacac gaacagcggc acccagaact cgtgctggcc gatgaacgcc
     3181 acgaacatca gctgcagcag gcccgcgaag cccgcggcgc tggaggccac cgccaggaat
     3241 gccgccaccg ggaccggcgc gccctcgtag gtgtcgggcg cccagaactg gaacggcacg
     3301 gcgctcacct tgaaggcgaa gcccaccacc acgaacagga tcgcggcgag cgccagcgtc
     3361 tcctgctggg ccggcagtgt cgagagcttc gccgcgatct cg
//
LOCUS       DATDBZ010000608         4223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2859303, whole genome shotgun sequence.
ACCESSION   DATDBZ010000608 DATDBZ010000000
VERSION     DATDBZ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2859303"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VIX62_14155"
     CDS             complement(<1..695)
                     /locus_tag="VIX62_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861420.1"
                     /translation="MSAVVEPAADDRPFAVVDIGSNSGRMIVFRLREGEHLDVVEDAR
                     APLRLARELRDSDRLGTDAIDRVVDALRDFLAVAQGAGATRMIAVATSAVRDAADGAE
                     LVERAQRLGIQLQTIDGDHEALLGFYGAVHDLPVTGGVTFDVGGGSAELTSFRSRRLL
                     RSWSMPLGSLRMSDRYLDSDPPTDHEIKALVKATGASLDDAGIAELAPGEELVGIGGT
                     VRNLAKIDLKRTD"
     gene            complement(735..1355)
                     /locus_tag="VIX62_14160"
     CDS             complement(735..1355)
                     /locus_tag="VIX62_14160"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY5861421.1"
                     /translation="MADAVEISGTKQRIVQATIDTIRDEGFASASARAIARRGDFNQA
                     LIFYHYGTLNELLLAAFDQVTAARIERYVEVFEGPDDLASKLRAMIPLYRDDRESGRL
                     RVITEVIAGATGRPDLAPEVAARIDPWISMTDHVVRSLLDDVGMTGVVEPPDAAFAIS
                     ALFVGMDVLGSLGYDDAPAERLIELAASLAPMLEMLSGLGAAAAED"
     gene            1586..2908
                     /locus_tag="VIX62_14165"
     CDS             1586..2908
                     /locus_tag="VIX62_14165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5861422.1"
                     /translation="MRRLRPQTVYLLYSGAEGFAFRLMATIFSVFLIVELGLDPLQLL
                     LMGTVLEGTYLLFEIPTGVVADTVSRRLSVVIGLFGSGIAFLLLAWSGSFLWAMVSQA
                     LWGVFATFQSGADIAWLTDEIGEEEARPLYLRSEQVWQGGALAGVVASVILASVNLRL
                     PIALAGLGFCALGLFMALAMREDGFTKPERSEGEHRRTAFTTTLKDGVGQVRAHHVLL
                     LILAVAALHGASTEGFDRLSDYHLLRDLGLPSLGDLDPVVWFGILDGVSMVLGIVALS
                     VVKRRAHLEGHAAVAKILMGIDALLIVSVVVFGLAGQFWMALLAFWVVGALRTVRDPV
                     FTAWINQGLDPKTRATINSMGSQADAVGQAAGGPVLGLVATKVSTPAALVTSGLLSLP
                     AIGLYIRAIRRGSVGTLKPEEMESEIRIDEADEIEAEVPEGPHTPSGA"
     gene            complement(2913..3221)
                     /locus_tag="VIX62_14170"
     CDS             complement(2913..3221)
                     /locus_tag="VIX62_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861423.1"
                     /translation="MSAIARLQTFAERAEISFDELLDFLDSPSGRRVRNAFAAGLIVS
                     VPMIMRIPGLRRSPIGRLVELTGGTAIVLALAEAIREWERSDKTKRRQRVIDVPPASP
                     "
     gene            complement(3281..3375)
                     /locus_tag="VIX62_14175"
     tRNA            complement(3281..3375)
                     /locus_tag="VIX62_14175"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3338..3340),aa:Sec,seq:tca)
     gene            3864..>4223
                     /locus_tag="VIX62_14180"
     CDS             3864..>4223
                     /locus_tag="VIX62_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861424.1"
                     /translation="MIALALVLPILPIGMQAAGASSNNALQFNGTSQYARIGISPTNA
                     LQLSTFTLETWFMRTGTGGTMTTGAVTGIPLITKGRGEGETQESRDVNYFLGISTAGT
                     GTTNVLAADFEEAASGSS"
BASE COUNT          667 a         1364 c         1467 g          725 t
ORIGIN      
        1 tagtcggtgc gcttcaggtc gatcttcgcc aggttccgga cggtaccgcc gatgcccacg
       61 agttcttctc cgggcgcgag ctcagcgatg cccgcgtcgt ccagggacgc tcctgtggct
      121 ttgacgaggg ccttgatctc gtgatcggtc ggggggtcgc tgtccaggta ccggtcgctc
      181 atgcggagcg acccgagcgg catcgaccac gatcggagca accgccggct ccggaacgag
      241 gtgagttcgg cgctgccgcc acccacatcg aacgtgacgc cgcccgtgac cggcagatcg
      301 tgaacggcgc cgtagaaccc gagcagtgct tcgtggtcgc cgtcgatcgt ctggagttgg
      361 atgccgagtc gctgcgcgcg ttccaccagt tcggcgccgt cggcggcgtc gcgcaccgcg
      421 gacgtggcga cggcgatcat gcgcgtggcg cccgcccctt gcgccaccgc caggaaatcg
      481 cggagcgcat cgaccacgcg gtcgatcgca tcggtgccga gacgatcgga atcccgcagt
      541 tcgcgggcga gtcgcaacgg tgctcgcgcg tcttcgacga cgtcgaggtg ctcaccttcg
      601 cgcagacgga acacgatcat ccgccccgag ttcgagccga tgtcgacgac cgcgaagggc
      661 cggtcgtccg ctgcgggttc cacgaccgcg ctcacggccc gatgctccca catgccggtg
      721 acgacagggt gaactcagtc ctcggcagcg gcggctccga gaccgctcag catctcgagc
      781 atcggtgcga gggacgccgc gagctcgatc aggcgttcgg cgggcgcgtc gtcgtagccg
      841 aggctcccga gcacgtccat gcctacgaac aacgcggaga tggcgaacgc ggcgtcgggc
      901 ggctcgacga ccccggtcat gcccacgtcg tcgagcagtg agcggacgac gtggtcggtc
      961 atcgagatcc acggatcgat cctggcggcg acctcgggcg ccaggtccgg ccggccggtg
     1021 gcaccggcga tcacctccgt gatgacccgc agccgcccgg actcacggtc gtcgcgatac
     1081 agcgggatca tcgcccggag tttcgatgcg aggtcatcgg gaccttcgaa gacctccacg
     1141 taccgttcga tccgggccgc ggtcacctgg tcgaaggcgg cgagcagcag ctcgttcaag
     1201 gttccgtagt gatagaagat cagcgcctgg ttgaagtcgc cccggcgagc gatcgcacgg
     1261 gcggacgcgg acgcgaagcc ctcgtcccgg atcgtgtcga tcgtggcctg cacgatccgc
     1321 tgcttggtgc cactgatctc cacggcgtcg gccatcgagt ttgagcatac gctcaatgcc
     1381 ggcaccgttt gactcgcctc ggtgcctggg cgagacttcg cctcgcacat gccagcgatc
     1441 tagcgacatg ccgagtcgat ccgcgcgggg cttttggaca gccccgcttc gtgcgtacct
     1501 cggaacgtcc ggtcgctgat gcgaaccgtg gcggtgccct ccgagcgctc gatcccagcg
     1561 gctgctcgcc gcgaaaggac ctgtcgtgcg acgactgcgc ccacagaccg tctacctgct
     1621 ctactccggg gccgaaggct tcgccttccg actcatggcc accatcttca gcgtgttcct
     1681 catcgtggaa ctcgggctcg acccgttgca gctcctgctg atgggcacgg tactggaagg
     1741 gacgtatctg ctgttcgaga tcccgaccgg cgtggtggcg gacaccgtga gccggcggct
     1801 gtccgtcgtg atcgggctgt tcggttccgg gatcgcattc ctcctgctgg catggtccgg
     1861 ttccttcttg tgggcgatgg tgtcgcaggc cctgtggggg gtgttcgcca ccttccaatc
     1921 gggggccgac atcgcgtggc tcaccgacga gatcggggag gaggaggcga gaccgctcta
     1981 cctgcgtagc gagcaggtgt ggcagggcgg ggcgcttgcc ggggtggtcg cgagcgtgat
     2041 cctggcctcg gtgaacctgc ggcttcccat cgcgctcgcc gggctggggt tctgtgctct
     2101 cggcctcttc atggcgctcg cgatgcggga ggacgggttc accaagcccg agcgttctga
     2161 gggtgagcat cgccgcacgg cgttcaccac gacgctcaag gacggcgtcg ggcaggtccg
     2221 cgcccatcac gtgctgctgt tgatcctggc cgtggcagcg ctccacggcg cctcgaccga
     2281 agggttcgac cgcctctcgg actaccacct gcttcgcgac ctcggccttc cttcgctggg
     2341 ggacctcgat ccggtggtgt ggttcggcat cctggacggt gtctcgatgg tgctggggat
     2401 cgtcgcgctc tcggtggtga agcgccgggc gcacctcgaa ggtcacgccg cggtcgcgaa
     2461 gatcctgatg gggatcgacg cgttgttgat cgtgagcgtc gtggtgttcg gtctggcggg
     2521 acagttctgg atggcgctgc tggcgttctg ggtggtgggt gcgctgagga cggtgcgcga
     2581 tccggtcttc accgcgtgga tcaaccaggg gctcgatccg aagacgcggg ccacgatcaa
     2641 ctcgatgggt tcacaggcgg acgccgtggg tcaggccgcg ggcgggccgg tcctggggct
     2701 ggtcgccacg aaggtctcga cgccggcggc gctggtcacc tccgggctgc tgagcctgcc
     2761 ggcgatcggg ctctacatcc gcgcgatccg acgcggttcg gtgggcacgc tgaagcccga
     2821 agagatggag tcggagatcc ggatcgacga ggcggacgag atcgaggccg aggtaccgga
     2881 agggccgcac acaccgtccg gggcctgatg gcttacggcg atgccggcgg aacgtcgatg
     2941 acgcgttgac gccgcttggt cttgtcgctg cgctcccatt cccggatcgc ttcggcgagc
     3001 gcgagcacga tggcggtgcc cccggtgagt tccacgagcc gtccgatggg tgaacgtcgc
     3061 agcccgggga tgcgcatgat catcggcacc gacacgatga gtccggcggc gaacgcattg
     3121 cggacgcgac gacccgaggg ggagtcgagg aagtcgagga gctcgtcgaa cgagatctcc
     3181 gcgcgctcgg cgaaggtttg cagtcgggcg atggcgctca tgcctccact gtacccaggc
     3241 atcgcggatc agaacgcact cgaggtgtat gggccgaccg tggcggaggc ggacgggaat
     3301 cgaacccgcc tgggagctcg cgcccccaca tcggttttga agaccgtggg gcccaccagg
     3361 aaccctgacg cctccgccga gagtgtagcc agccgctcct cgagggcgtt ccgccatttc
     3421 gatggacctc tcggtacggt tgcgtactga aggaacgggt ggttccggta ctgtaggccg
     3481 tcgtccatac cgcatatcgg ccacgacggc tacgtagcca gtcatgggga aaacgccggt
     3541 gatcggggag aacctgcagg tgaatagcct tgacctaccg acccatggac gtgcaggatt
     3601 acgccttctg cccgattgcg tcccccagaa gggggatgga agactgcgta ggctctgatt
     3661 cggaaggaag gaaccaggca cgacggatat ccacccacgg ggctgagctc aacgacctga
     3721 cccggggtgc ggacggagag agatgcaggg gcgcaccgct cgccgcgatc gacatcgtcg
     3781 atcgcggcac gccgcgctca cggaggtcta gagggctcat gagtcgaacg ccgtggcgag
     3841 gcaccacccg acggttcgcg ggcgtgatcg cgctcgccct ggtgcttccc atcctgccga
     3901 tcggtatgca ggccgctggc gcttcgtcca acaacgcgct ccagttcaac ggcacgagtc
     3961 aatacgcgcg catcggcatc tctcccacga atgcgctcca gctctcgacg ttcacccttg
     4021 aaacctggtt catgcgcacg gggacgggcg ggaccatgac cacaggggcg gtgaccggca
     4081 tcccgctcat caccaagggt cgtggtgaag gcgagacgca agagagccgt gacgtcaact
     4141 acttcctcgg gatcagcacg gccggcacag gaacgaccaa tgtccttgcc gccgatttcg
     4201 aggaggccgc atccggatcc agt
//
LOCUS       DATDBZ010000609         2397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2031494, whole genome shotgun sequence.
ACCESSION   DATDBZ010000609 DATDBZ010000000
VERSION     DATDBZ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031494"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VIX62_14185"
     CDS             complement(<1..877)
                     /locus_tag="VIX62_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HEY5861425.1"
                     /translation="MTQPSEHPRERIPRDATNDYTAEQAERRRAFVEEHSGARLDHVG
                     RYSCDPATLPGNIENFLGVAQVPIGVAGPLRINGEHARGDFYVPLATTEGTLVASYNR
                     GMRVLSESGGVTTTVVGDSMQRAPAFVLPDALRAREFGLWVDAHLDQIRLAAEATTRT
                     GRLTHITQHAVGPIRFLRCNYTTGDAAGQNMTGKATLAACEWIRANYPGELEYVLSGN
                     IDTDKKHSAINTLLTRGKRVVAEATLDRHVLAELMGVETRTMFRWRQVSNVGALLSGA
                     ANNGAHAANGIAALFI"
     gene            1063..1464
                     /locus_tag="VIX62_14190"
     CDS             1063..1464
                     /locus_tag="VIX62_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861426.1"
                     /translation="MQEPMHGVVLLDVSPGAGEAFERSFFEKVGHPAVVCHGPHEGSL
                     CPLLDGKGCADFEAAHGIVFELDLDQPQHLAIVRRYRELGREGLPIRVVATPEQADRY
                     RDELAGVEIWTHEPTVADLDGFAAEVEATDR"
     gene            1536..>2397
                     /locus_tag="VIX62_14195"
     CDS             1536..>2397
                     /locus_tag="VIX62_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5861427.1"
                     /translation="MLRTHDVANQVPPLAGYDVFGADAALVEALDRNGASWASADLHG
                     LGRLAGAPASLEQGRLAEANRPVLQTHDRYGNRIDVVEYHPAYHQLMTTAIGHGLHAA
                     PWSDGRSGAHVARAAKVITWYQVDGGHVCPISMTYSVIPALRHNARLATEWEPRICSR
                     SYDPADRPAPLKTGATCGMAMTEKQGGSDVRTNTTTARTIGDGEYLLIGHKWFCSAPM
                     SDGFLMLAQAPAGLSCFWVPRWTPDGGRNVIAIQRLKDKLGDRSNASSEIELLDTWGV
                     LVGDEGAGVRT"
BASE COUNT          389 a          790 c          844 g          374 t
ORIGIN      
        1 cgatgaacaa cgccgcgatg ccattggcgg cgtgggcgcc attgttcgcc gccccgctca
       61 gcaaggcgcc gacgttggac acctgacgcc agcggaacat cgtgcgggtc tcgaccccca
      121 tgagctcggc gagcacatgc cggtcgagcg tcgcctcggc cacgacccgc ttgccgcgcg
      181 tgagcaatgt gttgatcgcg gagtgtttct tgtcggtgtc gatgttcccc gacaggacgt
      241 actcgagctc acccgggtag ttcgcccgga tccactcgca ggcggcgagc gtggccttgc
      301 ccgtcatgtt ctgacccgca gcatcacccg tggtgtagtt gcagcgaagg aagcggatgg
      361 ggccgacggc gtgctgcgtg atgtgggtca gccgccctgt acgcgtggtg gcctctgccg
      421 ccaagcggat ctgatcgagg tgggcgtcga cccacagacc gaactcgcgg gcccggagcg
      481 catcggggag gacgaaggcg ggagcgcgct gcatggagtc gccgacgacg gtggtggtga
      541 cgccgccgga ctcgctgagc acccgcatgc cacggttgta gctggcgacc aacgtgcctt
      601 cggtcgtggc cagcgggacg tagaagtcgc cccgggcgtg ctcgccgttg atgcgcagcg
      661 ggccggcaac accgatcggc acctgcgcga cgccgaggaa gttctcgatg ttgccgggca
      721 gcgtggcggg atcgcacgag taccgcccga cgtggtcgag gcgggcgccg gagtgctcct
      781 cgacgaaggc gcgccgacgc tccgcctgct cggcggtgta gtcgttcgtg gcatcgcgcg
      841 ggatccgctc ccgtgggtgc tcggacggct gcgtcacgtc ggcgacctcc gctcgatgga
      901 tcggccccga ctgtacgcgc atgtggccag tggttcgtga ccatcgcctc tgtctcgctc
      961 cgctgcgatg cgcgcacgat ggacattcac acgaggggtc aaaccagacg gagagcggaa
     1021 caagacaacc aacgaagccg cgacgagtga aagaggtgtg agatgcaaga gccgatgcat
     1081 ggggtggtac tgctcgacgt gtcgcccggc gcgggtgagg cattcgagcg atccttcttc
     1141 gaaaaagtgg ggcacccggc cgtggtctgt cacggaccgc acgagggatc attgtgccca
     1201 ctactcgatg gcaagggctg cgccgacttc gaggctgcgc acggaatcgt gttcgagctc
     1261 gacctcgacc aaccgcagca cctggcaatc gttcggcggt accgcgagct cggtcgcgag
     1321 gggttgccca tcagggtggt cgcgacgccg gagcaggcgg accggtaccg cgacgagctc
     1381 gccggggtcg agatctggac gcacgagccg acagttgccg acctcgacgg cttcgcggcc
     1441 gaggtcgagg cgaccgaccg gtagccggcg cggtgaccac cacggtggcg tcggacggcc
     1501 gcgcgcgcac gtcctagcct ccgaccatga gcacagtgct gcgcacccac gacgtcgcca
     1561 atcaggtccc tccactcgcg ggatacgacg tcttcggcgc cgacgcggcg ttggtcgagg
     1621 cgctcgaccg gaacggtgcg agctgggcgt cggctgacct gcatgggctc ggccggcttg
     1681 ccggcgcacc tgcctcgctg gaacagggcc gcctggccga ggccaaccgg ccggtgctgc
     1741 agacccacga ccggtatggc aaccgcatcg atgtcgtcga gtaccacccc gcctatcacc
     1801 aattgatgac gacggcgatc gggcacggcc tgcacgccgc accgtggtcg gacgggcggt
     1861 ccggcgccca tgtggcacgg gcggccaagg tgatcacctg gtatcaggtg gacggcggcc
     1921 acgtctgccc gatctcgatg acgtattcgg tgatccctgc gttgcgacac aacgcacggc
     1981 tggcaaccga gtgggaacct cggatctgct cgcgctccta cgatccggcc gaccgccccg
     2041 caccactgaa gaccggcgcc acgtgtggga tggccatgac cgagaagcag ggcgggtccg
     2101 atgtccgtac caacaccacg actgcccgaa cgatcggcga cggcgaatac ctgctgatcg
     2161 gccacaagtg gttctgttcc gccccgatgt cggacgggtt cttgatgctc gcccaggcgc
     2221 cggccgggct gtcgtgcttc tgggtgccgc gttggactcc cgacggcggg cgcaacgtga
     2281 tcgccatcca acgcctcaag gacaagctcg gcgatcgctc gaacgcctcg agcgagatcg
     2341 agctgctcga cacctggggc gtcctcgtcg gcgacgaagg tgcgggcgtg cggacga
//
LOCUS       DATDBZ010000610         3093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2031968, whole genome shotgun sequence.
ACCESSION   DATDBZ010000610 DATDBZ010000000
VERSION     DATDBZ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031968"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VIX62_14200"
     CDS             complement(<1..378)
                     /locus_tag="VIX62_14200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY5861428.1"
                     /translation="MPLDNIVFWVFAPISVASAIGMVVMRNAVHAALFLVVNFFTIAV
                     MYLLLDAPFLFAVQIIVYTGAIMVLFLFVVMLLGVERGEPKHEPLIGQRAIAVILAVG
                     IITEIGVAVRAGIGFATRAPDGFD"
     gene            complement(434..832)
                     /gene="nuoI"
                     /locus_tag="VIX62_14205"
                     /pseudo
     CDS             complement(434..832)
                     /gene="nuoI"
                     /locus_tag="VIX62_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983359.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
     gene            complement(931..2073)
                     /gene="nuoH"
                     /locus_tag="VIX62_14210"
     CDS             complement(931..2073)
                     /gene="nuoH"
                     /locus_tag="VIX62_14210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY5861429.1"
                     /translation="MDWIDWAILAVRVIIVFFVLLIAVMLYIWMERKVIADMQTRVGP
                     MRAGPRGMLITLADGIKLFFKEGITPTNADKRVYLIAPILAMFPGFLAFCTIPFGQPV
                     ELFGRVVSFQLTDLNIGILWILAMTSITVYGVVLAGYSSGSNYPLLGAVRSSAQMISY
                     EVGMGLAIVAVVMYSGHLSMNEIVAAQGTVWNVIPQFPAFVVFLICALAETNRPPFDL
                     AEAESELVAGFHTEYSGIKFAMFFLGEYVSTVTIAAVGVTLFLGGWRGYWPFHGAWSW
                     IGPLLWFLLKVTAVIYIMILIRATLPRMRYDRLMNFGWKVLIPFGLAWVMITGVVVVL
                     PQEYGDRGRWLAAAVIVGAAALVSLVAPLIARPRTSPRDDREEVRT"
     gene            complement(2073..>3093)
                     /locus_tag="VIX62_14215"
     CDS             complement(2073..>3093)
                     /locus_tag="VIX62_14215"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY5861430.1"
                     /translation="ERPGAADAAWMFRLTTQAGIKGAYVTRRANDRGALRAGVHPSLL
                     PGGRTMDEAGEVEPSWGPIMNREPGRDAHAILQACADHEVDVLFCIGVDPLRDLPDAA
                     LARRALANVDHVVVQSLELGSLESYASAFLPAAPFIEKEGHVTTWEGRGQRLKPIRGA
                     QGISLPDWEIFASMALACGGDLGFESLEELHEEMGALLAPREVATSLTTSGSNPSPAP
                     DHLQLFTYPLLVDEGRLSERADELKAALEPEAFVEIHPDDASAAGIADGALAVITTDA
                     GSATLPARVTEHVAEGAIFVPFNQPGFAANTLLSGEMIARASIAPAGGSADATAEAEP
                     VGSEA"
BASE COUNT          567 a         1037 c         1003 g          486 t
ORIGIN      
        1 atcgaacccg tcgggcgctc tggtcgcgaa cccgatgccg gcccgaaccg cgaccccgat
       61 ctcggtgatg atgcccacgg cgaggatcac ggcgatcgcc cgttgaccga tcagcggctc
      121 gtgcttgggc tcaccacgtt cgacgcccag caacatcacg acgaacagga acagcaccat
      181 gatcgcgccg gtgtagacga tgatctgcac cgcgaacagg aacggcgcgt ccagcaacag
      241 gtacatcacc gcgatcgtga agaagttcac gaccaggaac agtgctgcgt gcacggcatt
      301 gcgcatgacg accatgccga tcgccgaggc gaccgagatc ggcgcgaaca cccagaacac
      361 gatgttgtcc aagggcacga gcgggtcagc cttccttgtc cacggggtcg gtgcccagcg
      421 ccggcggcgg ttccaggagc tgttccttgg tccagatcgc gtcctcgcgg ctcgtgaagg
      481 acaactcgaa gtagtcggtc aacgtcaggg cgcgagtggg acatgcctcc acgcagaagc
      541 cgcacatgat gcagcgcagg tagttgatct ggtagtcctt cgcgaaccgc tcgcccggac
      601 tcgtggggtc ctccggatcg ttgtccgccc cctcgaccca gatcgcatcg gcaggacatg
      661 cgtaagcgca cagctcgcac ccgatgcatt tctccagccc gttctcgtgc ttgttgagca
      721 ggtgccggcc gatgtgcgaa cgctccggga tcggcgcgat ctccttcggg tactgctcgg
      781 tgttgtctgg ccggaacgtc tgcttgaaca ccgtgcccat gcccttggcc atggcgacgg
      841 tggaatccca gatgccgccg cgctcgcgcg gatcgcgctc agtcggctcg tgctcggagg
      901 gaccggatcg ctccggcgtc tgttcctcgg tcatgtccgc acctcctccc gatcatcgcg
      961 cggcgaggtt cgcggtcgag cgatcaacgg cgccacgagc gagaccaggg cggcggcccc
     1021 cacgatgacg gcggctgcca gccagcgacc gcgatcgccg tattcctgcg gcagcaccac
     1081 gacgacgccg gtgatcatga cccacgcgag accgaacggg atcaacacct tccagccgaa
     1141 gttcatcaac cggtcgtagc gcatccgcgg cagcgtggct cggatcaaga tcatgatgta
     1201 gatcaccgcg gtcaccttga gcaggaacca gagcagcggc ccgatccatg accacgcgcc
     1261 gtggaacggc cagtagccgc gccacccgcc caggaacagc gtgacgccga ccgctgcgat
     1321 cgtgaccgtg ctgacgtact cgcccaggaa gaacatcgcg aacttgatgc ccgaatactc
     1381 cgtgtggaac ccagccacga gttccgactc agcttcggcc agatcgaacg gcggacggtt
     1441 cgtttcagcc agtgcgcaga tcaagaacac gacgaacgct gggaactgcg ggatcacgtt
     1501 ccacaccgtc ccctgggcag cgacgatctc gttcatcgac agatggccgc tgtacatgac
     1561 gaccgcgacg atcgcgagac ccatgcccac ctcgtagctg atcatctgtg cgctcgaacg
     1621 aaccgccccc aacaaggggt agttggaacc gctcgagtag cccgcaagca cgacgccgta
     1681 gaccgtgatc gaggtcatag ccaagatcca caagatgccg atgttgaggt cggtcaactg
     1741 gaacgacacg acgcgaccga acagctcgac aggttgcccg aacgggatcg tgcagaacgc
     1801 caggaacccg gggaacatcg ccaggatcgg cgcgatgagg tacacccgct tgtccgcgtt
     1861 ggtgggcgtg atgccttcct tgaagaagag cttgatgccg tcggcgaggg tgatcagcat
     1921 gccgcgaggc ccggcgcgca tgggaccgac tcgcgtctgc atgtcggcga tcaccttgcg
     1981 ctccatccag atgtagagca tcaccgcgat cagcagcacg aagaacacga tgatcacccg
     2041 aaccgccagg atggcccagt cgatccagtc catcaggcct cgctccccac gggctcggcc
     2101 tcggcggtcg cgtcggccga gcctcctgcc ggcgcgatac ttgcgcgcgc aatcatctcg
     2161 ccggacagca gcgtgttcgc cgcgaaccca ggctggttga agggcacgaa gatcgcgccc
     2221 tcggcgacgt gctcggtgac tcgggcgggc aaggtcgcgc taccggcgtc tgtggtgatg
     2281 acggcgaggg cgccatcggc gatgcccgcc gccgatgcgt catcagggtg gatctcgacg
     2341 aacgcctctg gctccagcgc agccttcagc tcgtcggcac gctcggacag tcggccctcg
     2401 tcgaccagca acgggtaggt gaacagctgc aggtggtcgg gcgccgggga cgggttcgat
     2461 cccgaggtgg tgaggctcgt cgcgacctcg cgcggagcca ggagggcgcc catctcctcg
     2521 tggagttcct cgagggattc gaagccgagg tcgcctccgc aggccagggc catgctcgcg
     2581 aagatctccc agtcgggcag gctgatgccc tgcgcgccgc ggatcggctt caaccgctga
     2641 ccacgacctt cccaggtggt gacgtggcct tccttctcga tgaagggagc cgcgggcagg
     2701 aacgcgctcg cgtaggactc gagcgaaccg agttcgagcg attgcacgac cacgtggtcg
     2761 acgttggcga gtgcacgcct cgccagcgcg gcgtcgggca ggtcgcggag cgggtcgacg
     2821 ccgatgcaga acaacacgtc gacctcgtga tcggcacacg cctgcaggat cgcgtgggcg
     2881 tcgcgaccgg gctcgcggtt catgatcggt ccccacgacg gttcgacctc gccggcttcg
     2941 tccatcgtgc ggccaccagg aaggagcgac gggtgcacgc cggctcgcag cgcgccgcgg
     3001 tcgttggcac ggcgagtgac gtacgcgccc ttgatccccg cctgcgtcgt gagccggaac
     3061 atccacgcgg cgtcggcggc gcccggccgt tct
//
LOCUS       DATDBZ010000611         3192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_202373, whole genome shotgun sequence.
ACCESSION   DATDBZ010000611 DATDBZ010000000
VERSION     DATDBZ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202373"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VIX62_14220"
     CDS             complement(<1..609)
                     /locus_tag="VIX62_14220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5861431.1"
                     /translation="MTLRIEFFQTMNYQGPISVDSWPAPPRACESEWAKLTYEHSLDE
                     CAAAHEAGFDALNFAEHHYSPKQLTPNPVVLAALAGRRFTDAQIGVFGTDLPLNNPVR
                     IAEEYSMLDNLLDGRLRIAMLRGTPNEYLTYGSNPWESRERYEEGTLLVQACFTEPEP
                     FGWEGRYYRYRNIAVWPRRVQDPHPRILISGNSKDGAIFAGRH"
     gene            complement(708..1715)
                     /locus_tag="VIX62_14225"
     CDS             complement(708..1715)
                     /locus_tag="VIX62_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY5861432.1"
                     /translation="MKNVTDPNLPGIESWLRPGGVLTAIEASELPRFQSGFPPQHMLL
                     TLIGEYGLHVGDPMPSASLVLILGEFGVTQAGARAAIARLARRGIFVPTRSGRTTAYA
                     MTERCSWMVAEGRRLTLEFTGALRSWDGHWTLVSYSIAQERRAQRALLRTRLRWLGFA
                     PLQDGLWVSPRPPGQQLHEALADLGVGACAVFVADPVRGPDRLDPLAAWDLDEARAAY
                     AEFIDEFKPVSQALRRGRVSASEALIARTRVTYRWFAFANSQAELPETLLPANWLGAV
                     ARQLFEELYDGLGPLAALRIAQHIGTHDAALAAGVRSFTCTEPIDDVAPASRRESSVA
                     E"
     gene            complement(2023..2265)
                     /locus_tag="VIX62_14230"
                     /pseudo
     CDS             complement(2023..2265)
                     /locus_tag="VIX62_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            2354..2992
                     /locus_tag="VIX62_14235"
     CDS             2354..2992
                     /locus_tag="VIX62_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5861433.1"
                     /translation="MVSTWDGPIDPPETPLAIAAHQLVSESCPAHVVAHCERSVRFAM
                     MLAHLAAIDVDAEVLYLGVIMHDLGLAPRFHSDARFDVGGANSARDWLVEAGMDMRRA
                     GAVWDIVALHASSAIAQHKSPETAVANAGISIDVRGVGADRLPIDAVRRVLDEFPRVG
                     FPAAFHQTLVDEVRRKPDTVRLSWMEGMAVRHVDGYRAADFEAALLDSSDFH"
BASE COUNT          513 a         1077 c         1097 g          505 t
ORIGIN      
        1 gtgcctgccg gcgaagatcg cgccatcctt gctgttgccg gagatgagga tgcgcgggtg
       61 tggatcctgc acgcgacggg gccacacggc gatgttgcga tagcggtagt agcgaccctc
      121 ccagccgaac ggctcgggct cggtgaagca ggcctgcacg agcagcgtgc cttcctcgta
      181 gcgctcgcgt gactcccacg ggttcgaccc gtacgtgagg tactcgttcg gtgtgccgcg
      241 cagcattgcg atgcgcagcc ggccgtcgag caggttgtcg agcatcgagt actcctcggc
      301 gatccgcact gggttgttca gcggcaggtc ggtgccgaac accccgatct gggcgtccgt
      361 gaagcgccgg ccggcgagcg ccgcgagcac aaccgggttg ggcgtcaact gcttcggcga
      421 gtagtggtgc tcggcgaagt tcaacgcgtc gaacccggcc tcgtgagcag ccgcacactc
      481 gtcgagcgag tgctcgtacg tgagcttcgc ccactcgctc tcgcaggcgc gtggcggtgc
      541 cggccacgag tcgacgctga tcggcccctg gtagttcatc gtctggaaga actcgatcct
      601 caacgtcacc cctcagtgct gcatccgtcg tacggcgcac ctggaccact atatacaaca
      661 cttcatcgag ctggaagagg caaatctgtc gcttatcgaa gcagtgccta ttctgcgacg
      721 ctcgattccc ggcgactggc gggcgcgacg tcgtcgatcg gctcggtgca ggtgaacgac
      781 ctgaccccgg cggcgagggc ggcgtcgtgg gtgccgatgt gctgcgcgat ccgcagcgcg
      841 gcaagcgggc cgagcccgtc gtacagctcc tcgaacaact ggcgcgcgac agcacccagc
      901 cagttggccg gcaacaaggt ctcggggagc tcggcctgac tgttcgcgaa ggcgaaccag
      961 cggtacgtca cgcgcgtccg cgcgatcaac gcctccgacg cgctcacgcg gccgcgccgg
     1021 agggcctgcg aaaccggctt gaactcgtcg atgaactcgg cgtaggcggc gcgggcctcg
     1081 tcgagatccc aggccgcgag cggatcgagg cggtcgggcc cgcgcaccgg atcggcgacg
     1141 aacacggcgc acgcaccgac cccgaggtcg gccagcgcct cgtgcagctg ctggcccggt
     1201 gggcggggcg acacccacag cccgtcctgc aacggcgcga aaccgagcca ccgcaggcgg
     1261 gttcgcagca gcgcccgttg ggcacgccgc tcctgggcga tggagtagct gacgagcgtc
     1321 cagtgcccgt cccacgatcg cagagcaccg gtgaactcga gcgtgaggcg ccgaccttcg
     1381 gccaccatcc aggagcaccg ctcggtcatc gcgtacgcgg tcgtgcggcc cgacctggtg
     1441 ggaacgaaga tgccgcggcg ggcgagtcgg gcgatcgccg cccgcgctcc ggcttgggtg
     1501 acgccgaact ccccgaggat cagcaccagc gacgcggacg gcatcggatc gccgacgtgg
     1561 aggccgtact cgccgatgag cgtcagcagc atgtgctggg gcggaaagcc cgactggaac
     1621 cgcgggagct cactggcctc gatggcggtc agcaccccac cgggccgaag ccacgactcg
     1681 atgccgggca ggttcggatc ggtcacgttc ttcattcagg tcgccccaaa cggaaaagat
     1741 agtcgacgga tcctttggcg ccggcactcg aacaagtcgc actttggcac cagctggcgg
     1801 ccgcaccaat gccatggatc tgacgagcga tgcccgtcag tcggatgccg gccgctcgaa
     1861 ctcgttgggg gtcgcggtcg gcttgacccg aaactcgggg aagcggcgct gtgggtcgta
     1921 ctcgtcgcgg atcgcggcga gccgcccgaa gttgccggcg gccatgaacg gcgccgagcg
     1981 gttgagtagg ttgacggtcg cgacaccggg cgaagcgatc gtcatctcgc gcaggcccga
     2041 gaggtcgatc aggagggcgt cgtcgtggag gctccaggcc gcccaggagt gcccgccgga
     2101 gcgcacggtc acaccccaac cacgctcgcg cgcgagtcgg actccggcga cgacgtcggc
     2161 ctcggtggtt gcctcgagca ccgcgctcgg gaaccgggcg gggcgccgat cgtcgaagat
     2221 cgcatcgacc cgggccacct cgtaatccgt gctgccccgc tcgatgaggc ggccgtcgaa
     2281 cacggcgcgg accctaacgg gtggccggca ctcgtcccgg atctcgatcg ccgtggtggg
     2341 tacgctccct ggggtggtgt cgacctggga tggaccgatc gatccgccgg agacccccct
     2401 cgcgatcgcg gcccatcaac tggtctcgga atcgtgccct gcgcacgtcg tcgcccactg
     2461 tgagcgcagc gtccggttcg cgatgatgct cgcccacctc gccgcgatcg atgtcgacgc
     2521 ggaggtgctg tacctcggcg tgatcatgca cgacctcggg ctcgcgccgc gctttcacag
     2581 cgatgcccgc ttcgacgtcg gtggcgccaa ctcggcccgc gactggctgg tcgaggcggg
     2641 gatggacatg cgccgggccg gagccgtgtg ggacatcgtt gcgctccacg cttcgagcgc
     2701 gatcgcgcag cacaagagcc ccgaaacggc ggtcgccaac gctggcatca gcatcgacgt
     2761 gcgcggtgtc ggtgccgatc ggctgccgat cgatgcggtg cgccgagttc tcgacgagtt
     2821 cccgcgggtt ggcttcccag cggcgttcca tcaaacgctc gtcgacgagg tccgtcgcaa
     2881 acccgacaca gtgcggctgt cgtggatgga gggcatggcc gtacgtcacg tcgatggcta
     2941 ccgtgccgcc gacttcgagg ccgccctgct cgactcgtcc gactttcact gaccgaccga
     3001 ggatttccca caccgacgtg tatccccggc cgtccggttg gttccttata gcgtaggtcg
     3061 attgggcgga tcgctcgctc ccccaatgcg aaggcctctg gattcaaggg tccccttcag
     3121 tcgaaggacc tctgcattcg aaggaggatg caatgacaac gatctttgcc gacgatctcc
     3181 agcccggaga cg
//
LOCUS       DATDBZ010000612         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_202929, whole genome shotgun sequence.
ACCESSION   DATDBZ010000612 DATDBZ010000000
VERSION     DATDBZ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202929"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VIX62_14240"
     CDS             complement(<1..724)
                     /locus_tag="VIX62_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5861434.1"
                     /translation="MNGGRGFLSAAWPPLLFGLGFIALWEAVVAVFDLKPYFLAPPSE
                     ILGQFTDNLGSVWDAVLVSGANALVGLLIGTALGVAMSFLLSRYLVLHEVVTPLAIAL
                     NAVPIFVLVAVLNNMFAITSEVPRRLMVTLVVYFIVLVNVAKGLTQVNQIHLELMRSY
                     AANDSAILRKLRVPNAVPYLFTALKIAAPAAVITAFVSEYFGGSQNGLGYGITSNIAI
                     SRNATAWAYVLGACLLGLTFYLI"
     gene            complement(721..1539)
                     /locus_tag="VIX62_14245"
     CDS             complement(721..1539)
                     /locus_tag="VIX62_14245"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5861435.1"
                     /translation="MTSDVDVPAVEVRGIDKVFNRDAANRVDALAGVDLTVRRGEFVS
                     LIGPSGCGKSTLLRLIANLLEPSAGELLVNGKPARQARLDQDYGMAFQQSGLFEWRTV
                     AKNIELPLELKGWDKAKRRARAAEKLELVKLPEFAEHLPWQLSGGMQQRIAIARALAA
                     HPPLLLMDEPFGALDEMTREHMQAELLRICAETHTTVVFVTHSIPEAVYLSTRVIVMS
                     PRPGRITDVVDVDLGGERGIDTREADVFFKKITEVREALRGIDMSGGARGIEDR"
     gene            complement(1536..>2103)
                     /locus_tag="VIX62_14250"
     CDS             complement(1536..>2103)
                     /locus_tag="VIX62_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015623379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5861436.1"
                     /translation="LAVLMTRFKVVERGLFPYLVVSQTVPLIALAPLVATWGGKVQIG
                     EFVWPRWMSVAVIGAFLAFFPIAVGALKGLSSAPQASLELMDSYAASWRQTLFKLRFP
                     AAVPYLVPAMRLAASAAVVGVVVAEISTGLRGGLGRLILEYAREATSDPAKVCTALFG
                     AAALGLAMAGLVAVLDLTMTRNRPKEAT"
BASE COUNT          385 a          692 c          728 g          298 t
ORIGIN      
        1 agatcaggta gaacgtgaga ccgagcaggc aggcaccgag cacgtacgcc cacgcggtcg
       61 cgttcctgga gatggcgatg ttactcgtga tgccgtagcc cagcccgttc tgcgatccgc
      121 cgaagtactc ggacacgaag gcggtgatca ccgccgccgg tgcggcgatc ttcagcgccg
      181 tgaagaggta cgggacggcg ttcggcaccc gcagcttgcg caggattgcc gagtcgttgg
      241 cggcgtacga gcgcatgagc tcgagatgga tctggttcac ctgtgtgagg cccttggcca
      301 cgttcacgag gacgatgaag tagacgacga gcgtcaccat cagccgccgg ggcacctcac
      361 tcgtgatggc gaacatgttg ttcagcacgg ccacgagcac gaagatcggc acggcgttga
      421 gcgcgatcgc gagcggggtc acgacctcgt ggagaacgag gtagcgactc aacaggaagc
      481 tcatcgcgac gccgagggcc gtgccgatga gcaggccgac gagggcgttc gctcccgata
      541 cgaggacggc gtcccacacc gagcccaggt tgtcggtgaa ctggccgagg atctcggatg
      601 gcggggcgag gaagtagggc ttcaggtcga acaccgcgac gacggcttcc cagagcgcga
      661 tgaagccgag gccgaacagg agcggcggcc acgcggcgct gaggaagccg cgcccaccgt
      721 tcaccggtcc tcgatcccgc gtgcgccgcc gctcatgtcg atgccacgta gcgcctcgcg
      781 gacctcggtg atcttcttga agaacacgtc ggcctcgcgg gtgtcgatgc cgcgctcgcc
      841 acccaggtcg acatcgacca cgtcggtgat gcggccgggc cggggggaca tcacgatcac
      901 gcgggtcgac aggtagacgg cctcggggat cgagtgcgtc acgaacacca cggtcgtgtg
      961 ggtctcggca cagatccgta ggagctcggc ctgcatgtgc tcacgggtca tctcgtcgag
     1021 cgcaccgaag ggctcgtcca tgaggagcaa cggcgggtgg gcggcgaggg cccgggcgat
     1081 cgcgatccgc tgctgcatgc ctcccgagag ctgccacggc aggtgctcgg cgaactcggg
     1141 cagcttgacg agctcgagct tctcggcggc gcgggcacgg cgcttcgcct tgtcccaccc
     1201 cttcagctcg agcggcagct cgatgttctt cgccacggtg cgccactcga acaaaccgga
     1261 ctgctggaac gccatcccgt agtcctgatc gaggcgggcc tggcgggccg gcttgccgtt
     1321 gacgaggagc tccccggccg acggttcgag caggttggcg atcaagcgca gcaacgtcga
     1381 cttgccgcat cctgagggac cgatgagcga cacgaactca ccccggcgaa ccgtgaggtc
     1441 gacacccgcg agcgcgtcga cgcggttggc agcgtcgcgg ttgaacacct tgtcgatgcc
     1501 acgcacctcc accgccggga catcgacgtc cgatgtcatg tggcctcctt cggccggttg
     1561 cgggtcatcg tgagatcgag cacggcgacg agccctgcca ttgccaaccc cagagcggcg
     1621 gcaccgaaca gcgccgtgca caccttcgcc ggatcgctcg tcgcctcccg ggcgtactcg
     1681 aggatcaggc gaccgagccc gccccgcaat ccggtcgaga tctcggcgac gacgacaccg
     1741 accacggcgg cggatgcggc aaggcgcatc gcgggcacga ggtacggcac ggcggccggg
     1801 aaacgcagct tgaacagcgt ctggcgccag gaggcggcgt agctgtccat cagctcgagc
     1861 gatgcctgcg gggcggatga caggcccttc agcgctccta ccgcgatcgg gaaaaacgcc
     1921 aggaacgcac cgatgaccgc gaccgacatc caccgcggcc acacgaactc gccgatctgg
     1981 accttcccac cccacgtcgc cacgagcggg gcgagcgcga tcaacgggac cgtctgggag
     2041 acgacgaggt acgggaacag gccgcgctcg acgaccttga agcgggtcat gagcacggcg
     2101 agg
//
LOCUS       DATDBZ010000613         3959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U135
            k141_2038716, whole genome shotgun sequence.
ACCESSION   DATDBZ010000613 DATDBZ010000000
VERSION     DATDBZ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962338
            Sequence Read Archive: SRR25158255
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 20:02:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,855
            CDSs (total)                      :: 2,816
            Genes (coding)                    :: 2,792
            CDSs (with protein)               :: 2,792
            Genes (RNA)                       :: 39
            tRNAs                             :: 38
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 5 of 24
            Pseudo Genes (incomplete)         :: 20 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 3 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038716"
                     /isolate="SMAG_U135"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="United Kingdom"
                     /lat_lon="53.47 N 2.24 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VIX62_14255"
     CDS             complement(<1..617)
                     /locus_tag="VIX62_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY5861437.1"
                     /translation="MGRHRSFFAVRPEDQDQRPIDRKTVRRVVAIFKPYRGKVSIVAL
                     AIVITSGLGVVNPLLIAKVFNDALFGDPPGNCSGVACPNLPLLYLYVGLMIAVPIVTS
                     IIGIGQTYMANLVGLRVMQDLRNSLYAHLQFMPLRFFTTTRTGEIQSRLANDVGGVQN
                     VVTDTASNVLANVVTIISTLIAMLILSVQLTLLSLALMPLFLWLTV"
     gene            complement(675..1280)
                     /locus_tag="VIX62_14260"
     CDS             complement(675..1280)
                     /locus_tag="VIX62_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEY5861438.1"
                     /translation="MGRRTTRILAAALFAVLLVALLAPEAGGSSVVTRRGFTCTVVGT
                     PMGEPLHGTPGRDVICGLGGNDDLRGLGGNDVLLGGNGGDFLKGGAGDDLARGAEGRD
                     TLIGDGGDDRLFGGGGIDDGTGLIGDDLLNGQGGNDSTLDGGPSGQDIVRGGPGNDAC
                     VATVDGAGDDVASGGPGNDTSFTDPGDVRHTLEHEASCFAE"
     gene            complement(1339..2028)
                     /locus_tag="VIX62_14265"
     CDS             complement(1339..2028)
                     /locus_tag="VIX62_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY5861439.1"
                     /translation="MSIRQLRAIGSDVTGCRACPRLVAWREQTARDKVARFADQSYWA
                     RPVPGFGDPEARVLILGLAPAAHGGNRTGRVFTGDRSGDFLYASLFRTGFANQPTSVS
                     RDDGLVLRGAFVSAVNRCAPPANKPTPQERDRCLPFLGREIAALAELRVIVALGSFAW
                     DGALRALRANGVSVPSPKPRFGHGTQAQVGPVTLMGCFHPSQQNTFTGKLTEPMMDAI
                     FDRAAALAHER"
     gene            complement(2072..3709)
                     /locus_tag="VIX62_14270"
     CDS             complement(2072..3709)
                     /locus_tag="VIX62_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY5861440.1"
                     /translation="MTVVSLIDIEKSFGSWPVLRGADLEVGEGARIGIVGPNGAGKST
                     MLRILSGEETPGAGEVVHRKQLVVATLEQNPDGDERTAEQTVMAARPDIAALDADLRA
                     VEVELARPEVVADLSKMDRVLVRQQELLDRWVEAGGPGLSGEARSLLVSLGIEGADLD
                     LPTTSLSGGQRKLVALAACLIQQPGLLLLDEPETHLDADRRAQLEAIVAEFPGAVVTV
                     SHDRYMLDETVSRIAELEDGVITMWPGNYSAYAVAKEIALKRQAQLYVTQQKEIARLE
                     EAIKRFKDWADRVVNERHIKQARNKQRQIDRMDKVDKPVFERRKIALEMRSAQRGGNK
                     IVTLRDISVAFDEDPVLMDLSVEIYRGERIGIIGANGAGKSVLGTVLAGMREPDAGTR
                     WAGPAVQIGYLAQGHETEPPHLTPIQFVRNTKPMYEEDAVALLGRFLFRYDQMRQPVG
                     TLSGGERTRLQLCLLMLSGANCLLLDEPTNHLDIESMEVLEGALEGYDGTVIVISHDR
                     YLLDRIPDRIVEVRDGRAFSSPGGYDDWLSARQQVRA"
     gene            complement(3752..>3959)
                     /locus_tag="VIX62_14275"
     CDS             complement(3752..>3959)
                     /locus_tag="VIX62_14275"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY5861441.1"
                     /translation="RSRAAADRLGFTFEGVFRQHMIVKGRNRDTAWFSITDTEWPARR
                     AAIETWLATDDPGSISLRDLTAAR"
BASE COUNT          606 a         1392 c         1349 g          612 t
ORIGIN      
        1 accgtgagcc acaggaacag gggcatcaac gccagcgaca ggagggtgag ctgcaccgac
       61 aggatcaaca tcgcgatcaa cgtgctgatg atcgtgacca cgttggcgag cacgttcgag
      121 gcggtgtcgg tgaccacgtt ctggaccccg cccacgtcgt tggcgagccg cgactggatc
      181 tcgccggtgc gcgtcgtggt gaagaaccgc aggggcatga actgcaggtg ggcgtacagg
      241 gagttgcgca gatcctgcat cactcgcagg cccaccaggt tggccatgta cgtctgaccg
      301 atgccgatga tgctggtgac gatcggcacc gcgatcatca ggcccacgta caggtacagc
      361 agcgggaggt tcgggcatgc cacgccggaa cagttgcccg gcgggtcgcc gaacagcgcg
      421 tcgttgaaga ccttggcgat caggagcggg ttgacgaccc ccaaccccga ggtgatgacg
      481 atggccagcg ccacgatgga gaccttgccc cggtagggct tgaagatggc cacgacccgc
      541 cgaaccgtct tgcggtcgat cggccgctga tcctggtcct cgggacgcac ggcgaagaag
      601 ctgcggtgtc gacccatgcg gacatggtaa gcgcctgccg agacacggct attcccgggg
      661 agcgacctgt ggacctactc cgcgaaacag gatgcttcgt gctccagggt gtggcggacg
      721 tcgccggggt cggtgaagga ggtgtcgttg cccggacctc ccgacgccac gtcgtcgccg
      781 gccccgtcga cggtggcgac acatgcatcg ttgcccggac cgccgcggac gatgtcctgg
      841 cccgacggcc cgccgtccag cgtggaatcg ttcccgcctt gaccgttgag caggtcgtcg
      901 ccgatcagcc cggtgccgtc gtcgatgccg ccaccgccga acagccggtc gtcgccgccg
      961 tcaccgatca gcgtgtcacg gccttcggcc ccccgcgcga ggtcgtcgcc ggccccgccc
     1021 ttcaggaagt cgccgccgtt gccgcccaag agcacgtcgt tgccgcccag cccccggagg
     1081 tcgtcgttgc cgcccagccc gcagatcaca tcgcgcccgg gggtgccgtg cagcggttct
     1141 cccatcgggg tgcccaccac cgtgcaggtg aagccgcggc gcgtcacgac cgacgagccg
     1201 ccggcctcgg gggcgaggag cgcgacgagc aggaccgcga agagcgcggc ggccaggatc
     1261 cgagtggtgc ggcgtcccat ggcgaacctc cccggtgtgg gttcgagatg cctacgctac
     1321 tcccgtgcca gggtcggctc aacgctcgtg cgccagcgcg gccgctcgat cgaagatcgc
     1381 gtccatcatc ggctcggtga gcttgcccgt gaacgtgttc tgctggctcg ggtggaagca
     1441 ccccatcaag gtcacgggtc cgacctgcgc ttgcgtccca tgtccgaacc gcggcttggg
     1501 tgagggaacg gacacgccgt tggcccggag ggctcgcagc gcgccgtccc acgcgaacga
     1561 ccccagcgcc acgatgaccc gcagctcggc cagcgcggcg atctcccgcc ccaggaaggg
     1621 caggcaccgg tcgcgctcct gtggcgtggg cttgttggcg ggcggagcgc aacgattgac
     1681 ggcggacacg aacgctcctc gcaggacgag accgtcatcg cgcgagacgc tggtgggctg
     1741 attggcgaag ccggttcgga acaacgatgc gtagaggaag tcgcccgatc ggtcgcccgt
     1801 gaagacgcgg cccgtccggt tcccgccgtg ggcggccggc gccaggccca ggatgagcac
     1861 gcgagcctcc gggtcgccga acccgggcac cggccgggcc cagtaagact gatcggcgaa
     1921 gcgagcgacc ttgtccctgg ccgtctgctc ccgccaggcc accagtcggg ggcacgcccg
     1981 gcaccccgtg acgtccgatc cgatggcccg gagctggcgg atgctcatgc cagcagcggg
     2041 aaccggccgg cggcagggtc gaaccggtcg ctcacgcgcg cacctgctgc cgcgcgctca
     2101 gccagtcgtc gtagccgccg ggcgaggaga acgcccggcc gtcgcggacc tccacgatgc
     2161 ggtcggggat ccggtccagg aggtagcggt cgtggctgat cacgatcacg gtgccgtcgt
     2221 atccctccag cgccccttcg agcacctcca tcgactcgat gtcgaggtgg ttcgtgggtt
     2281 cgtccaggag caggcagttg gcacccgaga gcatcaacag gcacagctgg agacgcgttc
     2341 gctcgccgcc gctcaacgtc cccaccggtt gccgcatctg gtcgtagcgg aacaggaacc
     2401 gccccagcag ggccaccgca tcctcttcgt acatcggctt ggtgttgcgc acgaactgga
     2461 tcggggtcag gtgcggcggc tccgtctcat ggccctgcgc caggtagccg atctgcacgg
     2521 cgggcccggc ccaccgcgtt ccggcatcgg gttcgcgcat cccggcgagc accgttccca
     2581 gcaccgactt gcccgccccg ttcgcgccga tgatgccgat gcgctcgccg cggtagatct
     2641 ccaccgacag atccatcagc accgggtcct cgtcgaaggc caccgagatg tcccgcaggg
     2701 tcacgatctt gttgccaccc cgttgggcgc tgcgcatctc cagcgcgatc ttgcgccgtt
     2761 cgaacaccgg cttgtcgacc ttgtccatgc ggtcgatctg gcgctgcttg ttccgcgcct
     2821 gcttgatgtg gcgttcgttc acgacgcggt cggcccagtc cttgaagcgc ttgatcgcct
     2881 cctccagtcg cgcgatctcc ttctgctggg tgacgtagag ctgcgcctga cgcttcaggg
     2941 cgatctcctt ggccacggcg taggcgctgt agttgcccgg ccacatcgtg atcacgccgt
     3001 cctcgagctc ggcgatccgc gagacggtct cgtccagcat gtaccggtcg tgcgagaccg
     3061 tgaccaccgc gcccgggaac tcggcgacga tcgcttcgag ctgcgcgcgg cgatcggcgt
     3121 ccaagtgggt ctcgggctcg tcgagcagca ggagtccggg ctgctggatc aggcaggccg
     3181 ccagcgccac cagcttgcgt tggccgcccg acagcgacgt cgtcggcagg tcgaggtcgg
     3241 ccccttcgat accgagcgac accagcaggc tccgggcctc gcccgacagg ccgggaccgc
     3301 cggcttccac ccaacggtcc agcagttcct gctgacggac gagcacgcga tccatcttcg
     3361 acaggtccgc cacgacctcg ggacgagcca gctccacctc caccgcgcgc agatcggcgt
     3421 cgagcgcggc gatgtccggg cgcgcggcca tcacggtctg ttcggcggtt cgttcgtccc
     3481 cgtccggatt ctgctccagc gtggcgacca ccagctgctt gcgatggacg acctcgccgg
     3541 cgccgggggt ctcctcgccc gacaggatgc gcagcatcgt ggacttgccg gcgccgttgg
     3601 gaccgacgat gccgatgcgc gcgccctcgc ccacctcgag atcggctccc cgcagcacgg
     3661 gccaggagcc gaaggatttc tcgatgtcga tcaggctgac gacggtcatg acctcaccac
     3721 gatagggaac agcgagcggt gcgaccgggg atcagcgggc ggccgtgagg tcccgcagcg
     3781 agatcgatcc cgggtcatcg gtcgccagcc acgtctcgat cgccgcgcgg cgggccggcc
     3841 actccgtgtc cgtgatcgag aaccacgccg tgtcgcggtt gcggcccttc acgatcatgt
     3901 gctggcggaa cacgccctcg aaggtgaacc cgagtcgatc ggcggcggcg cgcgaccgg
//
